LOCUS       DAXIEB010000001        65561 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000001 DAXIEB010000000
VERSION     DAXIEB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 65561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.001"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            39..590
                     /locus_tag="ACFOA3_000001"
     CDS             39..590
                     /locus_tag="ACFOA3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897090.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(867..1559)
                     /locus_tag="ACFOA3_000002"
     CDS             complement(867..1559)
                     /locus_tag="ACFOA3_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6897091.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1865..2422)
                     /locus_tag="ACFOA3_000003"
     CDS             complement(1865..2422)
                     /locus_tag="ACFOA3_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897092.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(2815..3657)
                     /locus_tag="ACFOA3_000004"
     CDS             complement(2815..3657)
                     /locus_tag="ACFOA3_000004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6897093.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3896..4816
                     /gene="pyrB"
                     /locus_tag="ACFOA3_000005"
     CDS             3896..4816
                     /gene="pyrB"
                     /locus_tag="ACFOA3_000005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6897094.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVYSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4826..5284
                     /gene="pyrI"
                     /locus_tag="ACFOA3_000006"
     CDS             4826..5284
                     /gene="pyrI"
                     /locus_tag="ACFOA3_000006"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6897095.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5349..6014
                     /locus_tag="ACFOA3_000007"
     CDS             5349..6014
                     /locus_tag="ACFOA3_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897096.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6077..7294)
                     /locus_tag="ACFOA3_000008"
     CDS             complement(6077..7294)
                     /locus_tag="ACFOA3_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6897097.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDLDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7478..7981
                     /gene="def"
                     /locus_tag="ACFOA3_000009"
     CDS             7478..7981
                     /gene="def"
                     /locus_tag="ACFOA3_000009"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6897098.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            8068..8994
                     /gene="fmt"
                     /locus_tag="ACFOA3_000010"
     CDS             8068..8994
                     /gene="fmt"
                     /locus_tag="ACFOA3_000010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6897099.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9073..10332
                     /gene="rsmB"
                     /locus_tag="ACFOA3_000011"
     CDS             9073..10332
                     /gene="rsmB"
                     /locus_tag="ACFOA3_000011"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6897100.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10298..10897
                     /locus_tag="ACFOA3_000012"
     CDS             10298..10897
                     /locus_tag="ACFOA3_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6897101.1"
                     /translation="MVFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10897..13017
                     /locus_tag="ACFOA3_000013"
     CDS             10897..13017
                     /locus_tag="ACFOA3_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6897102.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLTVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            13010..14287
                     /locus_tag="ACFOA3_000014"
     CDS             13010..14287
                     /locus_tag="ACFOA3_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6897103.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAALTRYEWPGNFDQLSSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPDEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14378..15565
                     /gene="dprA"
                     /locus_tag="ACFOA3_000015"
     CDS             14378..15565
                     /gene="dprA"
                     /locus_tag="ACFOA3_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6897104.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDSPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15591..16052
                     /locus_tag="ACFOA3_000016"
     CDS             15591..16052
                     /locus_tag="ACFOA3_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6897105.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16124..18430
                     /gene="topA"
                     /locus_tag="ACFOA3_000017"
     CDS             16124..18430
                     /gene="topA"
                     /locus_tag="ACFOA3_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6897106.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNSKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCTNYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18503..19198)
                     /locus_tag="ACFOA3_000018"
     CDS             complement(18503..19198)
                     /locus_tag="ACFOA3_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897107.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(19218..19769)
                     /locus_tag="ACFOA3_000019"
     CDS             complement(19218..19769)
                     /locus_tag="ACFOA3_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897108.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19888..20268
                     /locus_tag="ACFOA3_000020"
     CDS             19888..20268
                     /locus_tag="ACFOA3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6897109.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMEL
                     KHCKEMLEHKDKEIELLRKLTETV"
     gene            20357..20926
                     /gene="rsmD"
                     /locus_tag="ACFOA3_000021"
     CDS             20357..20926
                     /gene="rsmD"
                     /locus_tag="ACFOA3_000021"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6897110.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRSFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21049..21300
                     /locus_tag="ACFOA3_000022"
     CDS             21049..21300
                     /locus_tag="ACFOA3_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6897111.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21398..21481
                     /locus_tag="ACFOA3_000023"
     tRNA            21398..21481
                     /locus_tag="ACFOA3_000023"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21432..21434,aa:Tyr,seq:gta)
     gene            21512..21585
                     /locus_tag="ACFOA3_000024"
     tRNA            21512..21585
                     /locus_tag="ACFOA3_000024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21544..21546,aa:Gly,seq:tcc)
     gene            21595..21669
                     /locus_tag="ACFOA3_000025"
     tRNA            21595..21669
                     /locus_tag="ACFOA3_000025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21627..21629,aa:Thr,seq:ggt)
     gene            21718..22902
                     /gene="tuf"
                     /locus_tag="ACFOA3_000026"
     CDS             21718..22902
                     /gene="tuf"
                     /locus_tag="ACFOA3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6897112.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22909..22984
                     /locus_tag="ACFOA3_000027"
     tRNA            22909..22984
                     /locus_tag="ACFOA3_000027"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22942..22944,aa:Trp,seq:cca)
     gene            23099..23377
                     /gene="secE"
                     /locus_tag="ACFOA3_000028"
     CDS             23099..23377
                     /gene="secE"
                     /locus_tag="ACFOA3_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6897113.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            23379..23915
                     /gene="nusG"
                     /locus_tag="ACFOA3_000029"
     CDS             23379..23915
                     /gene="nusG"
                     /locus_tag="ACFOA3_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6897114.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            24016..24450
                     /gene="rplK"
                     /locus_tag="ACFOA3_000030"
     CDS             24016..24450
                     /gene="rplK"
                     /locus_tag="ACFOA3_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6897115.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            24450..25145
                     /gene="rplA"
                     /locus_tag="ACFOA3_000031"
     CDS             24450..25145
                     /gene="rplA"
                     /locus_tag="ACFOA3_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6897116.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFAQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            25375..25875
                     /gene="rplJ"
                     /locus_tag="ACFOA3_000032"
     CDS             25375..25875
                     /gene="rplJ"
                     /locus_tag="ACFOA3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6897117.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            25933..26304
                     /gene="rplL"
                     /locus_tag="ACFOA3_000033"
     CDS             25933..26304
                     /gene="rplL"
                     /locus_tag="ACFOA3_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6897118.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            26495..30673
                     /gene="rpoB"
                     /locus_tag="ACFOA3_000034"
     CDS             26495..30673
                     /gene="rpoB"
                     /locus_tag="ACFOA3_000034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6897119.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            30830..35005
                     /gene="rpoC"
                     /locus_tag="ACFOA3_000035"
     CDS             30830..35005
                     /gene="rpoC"
                     /locus_tag="ACFOA3_000035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6897120.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            35297..35554
                     /locus_tag="ACFOA3_000036"
     CDS             35297..35554
                     /locus_tag="ACFOA3_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897121.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            35551..35700
                     /locus_tag="ACFOA3_000037"
     CDS             35551..35700
                     /locus_tag="ACFOA3_000037"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6897122.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            35879..36250
                     /gene="rpsL"
                     /locus_tag="ACFOA3_000038"
     CDS             35879..36250
                     /gene="rpsL"
                     /locus_tag="ACFOA3_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6897123.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            36368..36838
                     /gene="rpsG"
                     /locus_tag="ACFOA3_000039"
     CDS             36368..36838
                     /gene="rpsG"
                     /locus_tag="ACFOA3_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6897124.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            36857..38962
                     /gene="fusA"
                     /locus_tag="ACFOA3_000040"
     CDS             36857..38962
                     /gene="fusA"
                     /locus_tag="ACFOA3_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6897125.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            39050..40234
                     /gene="tuf"
                     /locus_tag="ACFOA3_000041"
     CDS             39050..40234
                     /gene="tuf"
                     /locus_tag="ACFOA3_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6897126.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            40252..40563
                     /gene="rpsJ"
                     /locus_tag="ACFOA3_000042"
     CDS             40252..40563
                     /gene="rpsJ"
                     /locus_tag="ACFOA3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6897127.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            40684..40989
                     /locus_tag="ACFOA3_000043"
                     /pseudo
     CDS             40684..40989
                     /locus_tag="ACFOA3_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            41253..41897
                     /gene="rplC"
                     /locus_tag="ACFOA3_000044"
     CDS             41253..41897
                     /gene="rplC"
                     /locus_tag="ACFOA3_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6897128.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGISKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            41897..42517
                     /gene="rplD"
                     /locus_tag="ACFOA3_000045"
     CDS             41897..42517
                     /gene="rplD"
                     /locus_tag="ACFOA3_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6897129.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            42514..42828
                     /gene="rplW"
                     /locus_tag="ACFOA3_000046"
     CDS             42514..42828
                     /gene="rplW"
                     /locus_tag="ACFOA3_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6897130.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLASATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRRDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            42834..43667
                     /gene="rplB"
                     /locus_tag="ACFOA3_000047"
     CDS             42834..43667
                     /gene="rplB"
                     /locus_tag="ACFOA3_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6897131.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            43674..43952
                     /gene="rpsS"
                     /locus_tag="ACFOA3_000048"
     CDS             43674..43952
                     /gene="rpsS"
                     /locus_tag="ACFOA3_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6897132.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            43961..44290
                     /gene="rplV"
                     /locus_tag="ACFOA3_000049"
     CDS             43961..44290
                     /gene="rplV"
                     /locus_tag="ACFOA3_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6897133.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            44300..44992
                     /gene="rpsC"
                     /locus_tag="ACFOA3_000050"
     CDS             44300..44992
                     /gene="rpsC"
                     /locus_tag="ACFOA3_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6897134.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            44976..45392
                     /gene="rplP"
                     /locus_tag="ACFOA3_000051"
     CDS             44976..45392
                     /gene="rplP"
                     /locus_tag="ACFOA3_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6897135.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            45392..45583
                     /gene="rpmC"
                     /locus_tag="ACFOA3_000052"
     CDS             45392..45583
                     /gene="rpmC"
                     /locus_tag="ACFOA3_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6897136.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            45583..45846
                     /gene="rpsQ"
                     /locus_tag="ACFOA3_000053"
     CDS             45583..45846
                     /gene="rpsQ"
                     /locus_tag="ACFOA3_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6897137.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            46068..46436
                     /gene="rplN"
                     /locus_tag="ACFOA3_000054"
     CDS             46068..46436
                     /gene="rplN"
                     /locus_tag="ACFOA3_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6897138.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            46448..46771
                     /gene="rplX"
                     /locus_tag="ACFOA3_000055"
     CDS             46448..46771
                     /gene="rplX"
                     /locus_tag="ACFOA3_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6897139.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            46781..47320
                     /gene="rplE"
                     /locus_tag="ACFOA3_000056"
     CDS             46781..47320
                     /gene="rplE"
                     /locus_tag="ACFOA3_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6897140.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            47323..47628
                     /gene="rpsN"
                     /locus_tag="ACFOA3_000057"
     CDS             47323..47628
                     /gene="rpsN"
                     /locus_tag="ACFOA3_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6897141.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            47644..48036
                     /gene="rpsH"
                     /locus_tag="ACFOA3_000058"
     CDS             47644..48036
                     /gene="rpsH"
                     /locus_tag="ACFOA3_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6897142.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            48050..48583
                     /gene="rplF"
                     /locus_tag="ACFOA3_000059"
     CDS             48050..48583
                     /gene="rplF"
                     /locus_tag="ACFOA3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6897143.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            48597..48950
                     /gene="rplR"
                     /locus_tag="ACFOA3_000060"
     CDS             48597..48950
                     /gene="rplR"
                     /locus_tag="ACFOA3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6897144.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            48969..49487
                     /gene="rpsE"
                     /locus_tag="ACFOA3_000061"
     CDS             48969..49487
                     /gene="rpsE"
                     /locus_tag="ACFOA3_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6897145.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            49480..49665
                     /gene="rpmD"
                     /locus_tag="ACFOA3_000062"
     CDS             49480..49665
                     /gene="rpmD"
                     /locus_tag="ACFOA3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6897146.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            49667..50101
                     /gene="rplO"
                     /locus_tag="ACFOA3_000063"
     CDS             49667..50101
                     /gene="rplO"
                     /locus_tag="ACFOA3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6897147.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            50113..51423
                     /gene="secY"
                     /locus_tag="ACFOA3_000064"
     CDS             50113..51423
                     /gene="secY"
                     /locus_tag="ACFOA3_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6897148.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            51428..51646
                     /gene="infA"
                     /locus_tag="ACFOA3_000065"
     CDS             51428..51646
                     /gene="infA"
                     /locus_tag="ACFOA3_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6897149.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            51667..51780
                     /gene="rpmJ"
                     /locus_tag="ACFOA3_000066"
     CDS             51667..51780
                     /gene="rpmJ"
                     /locus_tag="ACFOA3_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6897150.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            51846..52208
                     /gene="rpsM"
                     /locus_tag="ACFOA3_000067"
     CDS             51846..52208
                     /gene="rpsM"
                     /locus_tag="ACFOA3_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6897151.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            52228..52623
                     /gene="rpsK"
                     /locus_tag="ACFOA3_000068"
     CDS             52228..52623
                     /gene="rpsK"
                     /locus_tag="ACFOA3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6897152.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            52643..53263
                     /gene="rpsD"
                     /locus_tag="ACFOA3_000069"
     CDS             52643..53263
                     /gene="rpsD"
                     /locus_tag="ACFOA3_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6897153.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            53289..54275
                     /gene="rpoA"
                     /locus_tag="ACFOA3_000070"
     CDS             53289..54275
                     /gene="rpoA"
                     /locus_tag="ACFOA3_000070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6897154.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            54299..54667
                     /gene="rplQ"
                     /locus_tag="ACFOA3_000071"
     CDS             54299..54667
                     /gene="rplQ"
                     /locus_tag="ACFOA3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6897155.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            54814..55527
                     /gene="minC"
                     /locus_tag="ACFOA3_000072"
     CDS             54814..55527
                     /gene="minC"
                     /locus_tag="ACFOA3_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6897156.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            55555..56370
                     /gene="minD"
                     /locus_tag="ACFOA3_000073"
     CDS             55555..56370
                     /gene="minD"
                     /locus_tag="ACFOA3_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6897157.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            56374..56637
                     /gene="minE"
                     /locus_tag="ACFOA3_000074"
     CDS             56374..56637
                     /gene="minE"
                     /locus_tag="ACFOA3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6897158.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            56641..57561
                     /gene="oxyR"
                     /locus_tag="ACFOA3_000075"
     CDS             56641..57561
                     /gene="oxyR"
                     /locus_tag="ACFOA3_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6897159.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(57558..57791)
                     /locus_tag="ACFOA3_000076"
     CDS             complement(57558..57791)
                     /locus_tag="ACFOA3_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897160.1"
                     /translation="MAIVRQGEATPYWFLLIHYTWHCIGTDENIHSDAKVEYLQDGSF
                     RGDAILKIDDDGNILAYRVVGSGKWRFENNACA"
     gene            58134..58523
                     /locus_tag="ACFOA3_000077"
     CDS             58134..58523
                     /locus_tag="ACFOA3_000077"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6897161.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILLFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(58894..61731)
                     /locus_tag="ACFOA3_000078"
     CDS             complement(58894..61731)
                     /locus_tag="ACFOA3_000078"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6897162.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADKYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDETG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLSAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLNKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(61828..62637)
                     /gene="zupT"
                     /locus_tag="ACFOA3_000079"
     CDS             complement(61828..62637)
                     /gene="zupT"
                     /locus_tag="ACFOA3_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6897163.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(62818..64074)
                     /locus_tag="ACFOA3_000080"
     CDS             complement(62818..64074)
                     /locus_tag="ACFOA3_000080"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6897164.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(64153..65544)
                     /locus_tag="ACFOA3_000081"
     CDS             complement(64153..65544)
                     /locus_tag="ACFOA3_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6897165.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
BASE COUNT        16859 a        15097 c        17144 g        16461 t
ORIGIN      
        1 ccttattttt tgttcggcgg cttgcgtggt cgggtaaaat gaaagttttg aacggttggt
       61 cggacaggaa gatgtggcgg gttttgagtg ctttgccgat aggcgtggtg ttttttgatt
      121 tgatctacgg ttttgtgttg aatgtgttgc agggtttgga tttgcagcgt gccgtgccgg
      181 attcggaagg cgtgttggcg gttacgcccg atattgcatt caacagtttg cagattgtcg
      241 ccaacggcgg tatggcggcg gtggtctgtt tcgggttggc ggttgtgttt ttgctcaacc
      301 gttcggtgcg gcggcggcag gtgttggaaa tcggggtgtt ccggatgttg gggctggtgg
      361 cggtattggc gttcagcgcg ccgtcggtgt gggagtgggc gaacgcgcta ccgctgctgc
      421 tgaagggcgc ggacgtggtc aatacgggga atgcgcgtta tgtgctgacg gctttgtgta
      481 tgccctttcc ggcggtgtcg tgcgtcatcg ggctggtggg gcggttcagg cttcagacgg
      541 catcgggcag ggcggcaaag tcagggggtg cgggcaaggc ggacggatag gacgcatttt
      601 tcagcgggtg cgtcgagaag cagccgatgt gtttggcagc cgcagcttgg gggggtgtag
      661 tgctaatggc ggtttctttg cttttatagt ggattaacaa aaaccagtac ggcgttgcct
      721 cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg
      781 tactattggt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
      841 aaaataaatg ggcaaaaatc ggtttattat cgtttttgcc gcatttggat ttgttctacc
      901 gtaaaacgtg tttgacgaac gggattctta ttaaaaaaca tctgatttct aacaaaatca
      961 gtattttttg gcacgatggc taaaattttt ccttccattt cgccatcacg tgttttccat
     1021 gcgctcaaga attgtgattt gctcattgag acgtgcccca gcgatggatc agccagcaaa
     1081 acagtttctc cgttaatacc gttcaatacc gaaaaatggt tgtttttacg gtattttaaa
     1141 tacacaatta caggaatttt tagttgtacc aactgttcaa atggcaaagc ataaccttgt
     1201 gcttcaaaac ccagttcggg cattatgcgt tgcatatcat caaaagaagc acgcatttgg
     1261 gttttatcca ttttgtccaa gatttccgct tcagaataat gtctgccata aaaattattc
     1321 agtaacgtgg caatcgaagc cgcgccgcaa gaaaaatcca aatcttgttt tactatgccg
     1381 gaatctcgcc gtgctttcca actccgtaca tggatgtttt ggtaagaagc ggggttttca
     1441 gcaaatatag ggtaagcaaa aacaatattt gggataaagc caattaaaac cgcatggttt
     1501 attaatttag aaatattttt tatcatagta tatatgcgta gaataaacgc atgaaacata
     1561 aaaaaataaa atcatatgca attttattgc atgaataaat atgaataaaa tagataatga
     1621 tgggagtaaa tacgccatgt attttggaag tttaaattta ttaataataa aataatttat
     1681 cgtagcgcaa ataaatccca aaattggaac gattagaaaa aaaattaata cacccatcat
     1741 catgcaaact ctatattaat aaatagctaa ctaattctat tgtggaaatt agttagctaa
     1801 ctaaaagtta ttaatgatta tttccgagaa ttgactgcat tgttggcagc attggcccca
     1861 aaacctagtg catgaatacc cggtctccat gccaaatttc cagccaatcc gcctccggca
     1921 gcagcagcca gccctgtttt gccgctactc ctgttgccgc accgattcct gccgcagtag
     1981 ccgcgccttg cgcagttcct aatttaccat gattatacaa attagcacca tgatactccc
     2041 atgcacctaa tgcaccgcca aaagcagcgg ctgcaataat gggaacaaat tcaccttgtg
     2101 tttctttcat ttcagcctgt gataattgaa ttgctttcac attttggctg tcaaaaactt
     2161 ggctgtctaa attttgcgcc attacaggtg taatcatcat agccattaca gttgcaattt
     2221 tcgttgcgct ggtttgcaca taaataggat tagcaaattc gctttgattg cgttcagtgt
     2281 tgatgtagct aatactgctt tctagtttga atttaccctt gtcagtcaat aattcttcca
     2341 aacttaaagg taagtcagca taagcaattt ggctggcaac agtcaaaata aaatctatta
     2401 gacatttgtg tttttgcatc atttcgtttg attttctagt ttttgagaat gatacaaagt
     2461 ttttttacaa agtaaagagt cactctgaaa aacatttttt cattataaat caaaatattg
     2521 atagaataaa tagcgagcat cgattcacgg tgtactttag tgcaaagcgt acaacgcgag
     2581 cctgaaccac cagccgcaac aggaaaagaa agccgataga gtgcaatgct tgccaacgtg
     2641 caagcgagct tgcaagaacg cttggctcaa cgagagcagg caagacagaa agcaggatag
     2701 gagcggtaac gcaaaggtct cgggctttga tttcgccgta aaccctgctg ccgccttgtc
     2761 cggaaagggt gcaggcggcg agtgccgaca gggtgcagat ggggaggggg ggtttcattt
     2821 ggggtcgcaa cggaagtggt atgcgcagat ttcaaaaccg tttttgaaat acaggcggtg
     2881 cgcgtcggca cggtcgtggt tgacgtggac gttgaggtgg attttggtta cccctgtttc
     2941 cgcgccgatt ttgcggactt cttccaaaag gcgcgaggcg tagcctttgc ggcggctttg
     3001 cggcagggta acgatgtcat cgatgtggat gtggcggccg ctggcgaggg tgcaggcttc
     3061 gcggaagccg cagacggcga cggcattgtg tttgccttct tcaaaaatac ccagcaggcg
     3121 gtagccttgg gggcgttgga ctttgttgat ctgttcggta aagcggttga tgtcggtcag
     3181 ggcggaacgc aaaacgctca aggctgcaaa ggcggtggcg gtgtcgtccg cgccgatttc
     3241 gcgcaaaacg taggatgcgc ccgaggcggt ctgttcctgt gctttctcgg cggcgtgttt
     3301 ttcttcgatt gcctgtgcca gcatgacgtg ttcgtcggca gggttgtttt gtccgccctg
     3361 ttcgcgttct tcgagcaggg ctttgcagtc gatgacgcgc aggtcgttgt cggcggcaaa
     3421 gtccatcagg aagcggaaca tttgggggtt gtcggtttcc agttttttat cgacggcgac
     3481 gcagcggatg ttgtccacca gcatggggcg cacccatttc gaataggaaa gcctgtggtc
     3541 tttggtgaac gaggacagaa tgccgcacag gcgttccgcc cagtcgctgg gacggaaaat
     3601 cttgccggaa ctcgttgtgc cgtggatgac gacttcgtag gggttgcaga ctaacatggc
     3661 ggcttcctga aaagaaatgt ctggcgcgat tataccttat gcttatgcgg gcgtgtttgg
     3721 atatgccgtc tgaaaagtac gggattcgtg cggtaaaact ttgcggcggc aaatgtgcga
     3781 taatacgcgc cgtattgccg cttttgcgaa gctgttccgc aaacatacgg gcggcgtgga
     3841 cgacgtataa ccggataccc gcctgacgcg ggttttttac ggaagggggg caaaaatgcc
     3901 taatccgctt tacagacagc atatcatctc catttcggat ttgtcgcgcg aacagttgga
     3961 atgcctgctt cagacggcat tgaagctgaa ggcgcatccg cgcggcgatt tgttggaagg
     4021 caaacttatc ggttcgtgtt tctttgaacc gtccacgcgc acgaggctgt cgtttgaaac
     4081 ggcggtgcag cggctgggcg gcaaggtcat cggcttttcg gacggcgcga ataccagtgc
     4141 caaaaaaggc gagacgcttg ccgataccgc ccgcatcatt tccggctaca ccgacgcaat
     4201 catccaacgc caccccaaag acggcgcggc gcgcgtggca gcggagtttt cgcgcgtccc
     4261 cgtcatcaac gccggcgacg gcaccaacca gcaccccagc cagaccttgt tggatctggt
     4321 aacgatttat gaaacgcagg ggcgtttgga caagctcaaa atcgccatgg cgggcgactt
     4381 gaaatacggg cgcaccgtgt attcgctttg tcaggcgttg aaacgctggg gctgcgaatt
     4441 tgcctttgtt tcgccgccca gcctcgcgat gcccgactac attaccgaag agttggacga
     4501 agccggctgc cgataccgta tcctcggtag tttggaagaa gcggcggaat gggcggatat
     4561 cctgtatatg acccgcgtcc agcgcgaacg tttcgacgaa caggaatttg ccaaaatcca
     4621 aggcaaattc aatctcgacg catccatgct tgcccgcgcc aaaccgaacc tgcgcgtgct
     4681 gcaccctttg ccgcgcgtgg acgaaatcca tcccgatgtc gatgctacgc cgcatgccta
     4741 ttatttcgag caggcgacca acggcgttta tgcgcgtatg gcgatattgt cgctggtgtt
     4801 gaacgaagaa gtgtgaggaa ccgatatgga aaccccgaaa ctcagtgtcg aagccattga
     4861 aaaaggtacg gttatcgacc atattcccgc cggcaggggg ctgaccatcc tgcgccagtt
     4921 caaacttttg cactacggca acgcggtaac cgtgggcttc aacctgccca gcaaaaccca
     4981 aggcagcaaa gacatcatca aaatcaaagg cgtgtgcttg gacgacaaag ccgccgaccg
     5041 cctcgccctg ttcgcccccg aagcggtggt caataccatc gacaatttca aggtcgtgca
     5101 gaagcggcat ttgaacctgc ccgatgaaat tgccgaagtg ttccgttgcc cgaacacgaa
     5161 ttgcgccggc cacggcgagc cggtcaaaag ccggttttat gttaaaaaac acaacgggca
     5221 gacgcggctg aaatgccatt actgcgaaaa aacctacagc cgggattcgg tggcggaagc
     5281 ctgacggatt cccttaaacc gagcgggcgg catttcgtct gccgcttgtt ttgccaatct
     5341 gaaatggaat gatgatgcac gcttctgtcc aaagccgttt cgcaccgata ctttatgttt
     5401 tgattttctt tgccggtttt ttgaccgcgc aaatttggtt caatcagaaa gcctatactg
     5461 aagagctgcc tccgcttctg tccgcattgt ccgccgtcgc gctggtgtgg ctggcgtggg
     5521 cgttcgtgtc ggcgcgttca aaggctaagg cggaaaagtt ctaccgcgaa aaaatgatac
     5581 agaacgaaag catacacccc gtcctgcacg cttctttgca acacttggaa cacaagccgc
     5641 aaatgctcgc cctgctggtc aaaaaccacg gcaaagggat ggcggaacag gtcaggttca
     5701 aggcggaagt gctgcccgac gacgaagacg cgcgcacgat tgccgccgag ttggcaaaaa
     5761 tggatatgtt tgcattgggg acggacgcgg tcgcctcggg cgaaacctat ggacgcgtgt
     5821 tcgccgatat tttcgagttg tcggcggctt tggaagggcg cgcgttcaaa ggaatgttga
     5881 aactgacggc ggaatataaa aacatcttcg gcgatgcctg ccgttcggaa acggcgttgg
     5941 atttgggcgc gctcaatcag gcgttgcagg agatttcaaa aacatcggaa aagtccaaac
     6001 ggatatttta ttgaagatgg aaaaatgccg tctgaaacgg aaggtgtttc agacggcatt
     6061 tttgtcggat gattaattat tcggagcggt tgaagccaaa cttcacgcgg ctgcggccct
     6121 gatccggtat attgtccaaa tcgcgtcccg gattggcggc ggtgtcgcct acggaaatat
     6181 cggagatgtt ttccaaaatg atggcggacg acaggtgttc ggaggtgcgg taaaccattg
     6241 ccaagcccac ttcttcggca ggagtggaaa tcagttcgac ggtatccctg cttttgaaat
     6301 tgttggagag gtcgacctgc atcgttttct tgcgtttgta gaggctcaaa accgtgcctt
     6361 tgtccaaacc gtccgcctcg cctttgtcga tggtgatggt ttgaaactgg ccggcaatcc
     6421 ttgtgccttc aaacacggaa acgattttag cctgaaccgg gcgggacggt tcgtgcggca
     6481 tcatgttgaa gcggtcggtg tcttccggca ttttcatcag gtagtcgccc tgctgtattt
     6541 cggaaatggc ggtttcgacc accagcggct gtatcgaagg ggtgcgcagc ggggtaatca
     6601 aaggatgggt gcgggtatgg tattcgttgt ctttcggccg ttctccagcc tgtttcgagc
     6661 gttgttcgag gacagagtcg gtatagtcga gggagcgcac gatgccgctg aatgcgactt
     6721 cctgcccgag gaatttaccc gtatccagat cggtgatgtt tttattgatt cggtaggtca
     6781 ggtagcggcc cggctctttc aggcctttgg tgtaaaccct ggtgcctttg gtgtacagca
     6841 gcctgccttc cgggcccgag agcaggcgcg gcgcggcagc ggtttctttg cgggaaacga
     6901 tttgcggatg ccgcataaag atgcggtaga agttgacatc gatggcggga ataccgtatc
     6961 cggacacttc cttatccgga ctcattttga cgacggggat gccgtctgtc tgttccaagc
     7021 cgaggcgcgg ttcgccgtca acgtggcgca acaccaatac ctggtccgga taaatcaggt
     7081 cgggattgtg gatttgatcc cggttcgcgt cccacaggcg gctccattgc cacgggctgt
     7141 acaggtattt gcccgaaatg ccccacaggg tgtcgccctg tttgaccgtg tagcgttccg
     7201 gcgcgttcgg gcgcacctcc aaatttgccg ccaaagtttg tgttgagaat gccatacctg
     7261 ccgcgcagag cagggttata atacgacgtt gcataaccgt tccccttatc tgataaattt
     7321 cggtttgtct tgcttgattg ggttggaaaa agcggcggca gcccctcggg atgtgccgcg
     7381 tgataaaaaa tgttccgcat tttaacatcg aattatccgc accatcacgg taattatgaa
     7441 aaacaggcgg cgtatccgcc gaaggaaaga gaaaattatg gctttattga atatcttgca
     7501 atatcccgac gagcgtctgc acacggtggc aaagcctgtc gaacaagtcg acgagcgcat
     7561 ccggaagctg attgccgata tgtttgaaac gatgtacgaa tcgcgcggca tcgggctggc
     7621 ggcgacgcag gtcgatgtgc acgagcgcgt ggtcgtgatg gatttgaccg aagaccgcag
     7681 cgaaccgcgc gtgttcatca accccgtcat cgttgaaaaa gacggcgaaa ccacttacga
     7741 agagggctgc ctgtccgtgc cgggcattta cgacaccgta acccgcgccg aacgcgtcaa
     7801 ggtcgaggct ttgaacgaaa aaggcgaaaa gttcacgctg gaggcggacg gcttgctggc
     7861 gatttgcgtg cagcacgagt tggaccacct gatgggcatc gtgtttgtcg aacgcctgtc
     7921 gcagctcaag cagggacgca tcaaaaccaa gctgaaaaaa cgtcagaaac atacgatttg
     7981 acccttttgc cgtgccgtct gaacgctgca aagttttcag acggcacggt cttgtccgac
     8041 aaccttacgc atacgcagga atatgctatg aaagtcatct ttgccggcac gcccgatttt
     8101 gccgccgccg ccttaaaagc cgttgccgcc gccggttttg aaatcccgct ggtgctgacc
     8161 cagcccgacc gtccgaaagg gcgcggtatg caactgaccg ccccgcccgt caaacaagcc
     8221 gcgctggaac tcggtttgcg cgtggcacag cccgaaaaac tgcgtaacaa cgccgaagcc
     8281 ctgcaaatgc tcaaagaggt cgaggcagac gtaatggtgg ttgccgccta cggtttgatt
     8341 ctgccgcagg aagtgttgga tacgccgaaa cacggctgcc tcaacatcca cgcttcgctg
     8401 ttaccccgtt ggcgtggcgc ggcgccgatt caacgcgcga ttgaagccgg cgatgccgag
     8461 acaggcgtgt gtattatgca gatggacatc ggtttggaca ccggcgatgt ggtcagcgaa
     8521 caccgctacg ccatccaacc gaccgatacc gccaacgaag tccacgacgc gctgatggaa
     8581 atcggtgcgg cggcggttgt tgccgatttg caacagcttc aaagcaaagg ccgtctgaac
     8641 gcggtcaaac agcccgaaga aggtgttact tacgcccaaa aactaagcaa agaagaggcg
     8701 cgtatcgatt ggagcaaaag cgcggcggtt atcgaacgca aaatccgcgc cttcaacccc
     8761 gtgcctgccg cgtgggttga gtatcagggc aagccgatga aaatccggcg cgccgaagtg
     8821 gtggcgcaac aaggcgcggc aggcgaagtg ttgtcctgtt cggcggacgg tttggtcgtt
     8881 gcctgcggcg aaaacgcgct gaagattacc gaattgcagc ctgccggcgg caggcggatg
     8941 aatatcgcgg cgtttgcagc aggacggcat atcgaagcag ggacgaagct gtaaatccct
     9001 tcagacggca ttccgatccg caaacgggaa tgccgcctga aaccatcagt cgaagaaagc
     9061 gaatcacata atatgagtat ggcacttgcc caaaaacttg ccgccgacag cattgcggcg
     9121 gttgccgaag gacgcaacct tcaggacgtg ttggcgcaaa tccgcaccgc gcatcccgac
     9181 cttacggcgc aggaaaacgg cgcgttgcag gacatcgcct acggctgcca gcgttatttg
     9241 ggcagtttga aacatatgct cgcgcagatg ctgaaaaagc cgattggcaa tccgcagctc
     9301 gaaagtctgc ttttggcggc gttgtaccaa ctgcattaca cgcgcaacgc gccgcatgct
     9361 gtggtcaacg aagctgttga aagcatcgcc aaaatcggac gcgggcagta ccgttcgttt
     9421 gccaacgcgg ttttgcgccg ctttttgcgc gaacgcgaca agcttgcggc ttcctgtaaa
     9481 aaagacgatg tggcgaaaca caacctgccg ctgtggtggg tggcttacct gaaaaaccat
     9541 tatccgaaac actggcacaa catcactgcc gcgctgcaat cccatccgcc gatgacgttg
     9601 cgcgtcaacc gccgacacgg caatgccgaa agctatttgg aaaaactggt ggcggaaggt
     9661 atcgcggcta aggcgttgga cgaatatgcg gttacgttgg aagaagccgt gccggtaaac
     9721 cgtctgcccg gtttttcaga cggcattgtt tcggtacagg acttcggcgc gcagcaggcg
     9781 gcgtatctcc ttaacccgaa agacggcgaa cggattttgg acgcgtgcgc cgcgccgggc
     9841 ggcaagacgg ggcatatttt ggaactggcg gattgccgcg ttaccgcctt ggacatcgat
     9901 gcaggccgtc tgaagcgtgt ggaggacaat atcgcccgtt tgggctttca gacggcatcg
     9961 gcggcgtgtg ccgatgcgcg ggatttggcg gcgtggtatg atggaaagcc tttcgacgca
    10021 atccttgccg acgtgccatg caccgcttcc ggcgtggcgc ggcgcaatcc cgacgtgaaa
    10081 tggctgcgcc gtccgaccga cgcgctaaaa accgcccgcc agcaggaagc cctgctggac
    10141 gcactttggc agacgctgaa acagggcggc agaatgctgc ttgccacctg ttccgtgttc
    10201 gtcgaagaaa acgacgggca attgcaaaaa ttcctcaacc gccatgccga tgccgaaccg
    10261 atcgaatcgc gggtgctttt accgaacaaa caccaagatg gtttttatta cgcgcttatt
    10321 caaaagcatt aaacaatggc ttgtgttgct gccgatgctt tccgttttgc cggacgcggc
    10381 ggcggagggg atagatgtga gccgcgccga agcgaggata accgacggcg ggcagctttc
    10441 catcagcagc cgcttccaaa ccgagctgcc cgaccagctc caacaggcgt tgcgccgggg
    10501 cgtgccgctc aactttacct taagctggca gctttccgcc ccgataatcg cttcttatcg
    10561 gtttaaattg gggcaactga ttggcgatga cgacaatatt gactacaaac tgagtttcca
    10621 tccgctgacc aaccgctacc gcgttaccgt cggcgcgttt tcgacagact acgacacctt
    10681 ggatgcggca ttgcgcgcga ccggcgcggt tgccaactgg aaagtcctga acaaaggcgc
    10741 gctgtccggt gcggaagcag gggaaaccaa ggcggaaatc cgcctgacgc tgtccacttc
    10801 aaaactgccc aagccttttc aaatcaatgc attgacttct caaaactggc atttggattc
    10861 gggttggaaa cctctaaaca tcatcgggaa caaataatgc gccgttttct accgatcgca
    10921 gccatatgcg ccgtcgtcct gttgtacgga ctgacggcgg caaccggcag caccagttcg
    10981 ctggcggatt atttctggtg gattgttgcg ttcagcgcaa tgctgctgct ggtgttgtcc
    11041 gccgttttgg cacgttatgt catattgctg ttgaaagaca ggcgcgacgg cgtattcggt
    11101 tcgcagattg ccaaacgcct ttccgggatg tttacgctgg ttgccgtact gcccggcgtg
    11161 tttctgttcg gcgtttccgc acagtttatc aacggcacga ttaattcgtg gttcggcaac
    11221 gatacccacg aggcgcttga acgcagcctc aatttgagca agtccgcatt gaatttggcg
    11281 gcagacaacg ccctcggcaa cgccgtcccc gtgcagatag acctcatcgg cgcggcttcc
    11341 ctgcccgggg atatgggcag ggtgctggaa cattacgccg gcagcggttt tgcccagctt
    11401 gccctgtaca atgccgcaag cggcaaaatc gaaaaaagca tcaatccgca taagctcgat
    11461 cagccgtttc cggataaggc gcgttgggaa aaaatccaac aggcgggttc ggtcagggat
    11521 ttggaaagca taggcggcgt attgtacgcg cagggctggc tgtcggcggg tacgcacaac
    11581 gggcgcgatt acgccttgtt tttccgtcag ccggttccca aaggcgtggc agaggatgcc
    11641 gtcttaatcg aaaaggcaag ggcgaaatat gctgagttga gttacagcaa aaaaggtttg
    11701 cagacctttt tcctggcaac cctgctgatt gcctcgctgc tgtcgatttt tcttgcactg
    11761 gtcatggcac tgtatttcgc ccgccgtttc gtcgaacccg tcctatcgct tgccgagggg
    11821 gcgaaggcgg tggcgcaagg cgatttcagc cagacgcgcc ccgtgttgcg caacgacgag
    11881 ttcggacgct tgaccaagtt gttcaaccac atgaccgagc agctttccat cgccaaagaa
    11941 gcagacgagc gcaaccgccg gcgcgaggaa gccgccagac attatcttga atgcgtgttg
    12001 gaggggctga ccacgggcgt ggtggtgttt gacgaacaag gctgtctgaa aaccttcaac
    12061 aaagcggcgg aacagatttt ggggatgccg cttacccccc tgtggggcag cagccggcac
    12121 ggttggcacg gcgtttcggc gcagcagtcc ctgcttgccg aagtgtttgc cgccatcggc
    12181 gcggcggcag gtacggacaa accggtccat gtgaaatatg ccgcgccgga cgatgccaaa
    12241 atcctgctgg gcaaggcaac cgtcctgccc gaagacaacg gcaacggcgt ggtaatggtg
    12301 attgacgaca tcaccgtttt gatacacgcg caaaaagaag ccgcgtgggg cgaagtggcg
    12361 aagcggctgg cacacgaaat ccgcaatccg ctcacgccca tccagctttc cgccgaacgg
    12421 ctggcgtgga aattgggcgg gaagctggat gagcaggatg cgcaaatcct gacgcgttcg
    12481 accgacacca tcgtcaaaca ggtggcggca ttgaaggaaa tggtcgaagc attccgcaat
    12541 tatgcgcgtt ccccttcgct caaattggaa aatcaggatt tgaacgcctt aatcggcgat
    12601 gtgttggcat tgtatgaagc cggtccgtgc cggtttgcgg cggagcttgc cggcgaaccg
    12661 ctgacggtgg cggcggatac gaccgccatg cggcaggtgc tgcacaatat tttcaaaaat
    12721 gccgccgaag cggcggaaga agccgatgta cccgaagtca gggtaaaatc ggaagcgggg
    12781 caggacggtc ggattgtcct gacggtttgc gacaacggca aagggttcgg cagggaaatg
    12841 ctgcacaacg ccttcgagcc gtatgtaacg gacaaaccgg cgggaacggg attgggtctg
    12901 cctgtggtga aaaaaatcat tgaagaacac ggtggccgca tcagcctgag caatcaggat
    12961 gcgggtgggg cgtgtgtcag aatcatcttg ccaaaaacgg taaaaactta tgcgtagcag
    13021 cgatatttta attgtagacg acgaaatcgg catccgcgac ctgctgtcgg aaatcctgca
    13081 ggacgaaggt tattcggtcg cattggcgga aaacgccgaa gaggcgcgca agctgcgcca
    13141 tcaggtacgc ccggcgatgg tgttgctgga tatttggatg cccgattgcg acggcatcac
    13201 ccttttgaag gagtgggcga aaaacgggca gctcaatatg ccggtggtga tgatgagcgg
    13261 gcatgccagc atcgataccg ccgtagaagc gaccaaaatc ggcgcgctcg actttttgga
    13321 aaaaccgatt tccctgcaaa agctgctgtc tgccgtcgaa aacgcgttga aacacggtgc
    13381 ggcgaaaatc gaaacgggac cggtgttcga caagctgggc aacagtgcgg cgattcagga
    13441 aatgaaccgt gaggtagggg ctgcggtgaa acgtgcctct cccgtacttt tgacgggcga
    13501 ggcgggttcg ccgtttgaaa cggtggcacg ctatttccat aaaaacggta cgccgtgggt
    13561 cagcccggca agggtcgaat atctgatcga tatgccgatg gaactgttgc agaaggcgga
    13621 gggcggcgtt ttgtatgtcg gcgacatcgc ccagtacagc cgcaacatcc aagccggtat
    13681 tgcctttatt gtcggcaagg cggaacaccg ccgcgtcagg gtggtcgcat cgggcagcag
    13741 ggcggcaggt tcagacggca ttgcctgcga ggaaaagctg gcggaactgc tgtcggagtc
    13801 ggtcgtccgc attccgccgc tgcgtatgca gcatgaagac attcccttcc tgatacaggg
    13861 gattgcctgc aatgtggcgg aaagccaaaa gattgcgccc gccgcattca gtgaagatgc
    13921 acttgccgca ttgacccgtt atgaatggcc gggaaatttc gaccagttga gcagtgtcgt
    13981 tgcaacgctg ctgttggagg cggacggaca ggaaatcggc gcggcggcgg tttcttccct
    14041 tttggggcag aatgtgcctg acgagggggc ggaagatatg gtgggcgggt ttaatttcaa
    14101 cctgcccctg cgcgaattga gggaggaggt ggagcggcgt tatttcgagt accacattgc
    14161 ccaagaaggt cagaatatga gcaaagtggc gcagaaggtc ggtttggaac gcacgcacct
    14221 ttaccgcaaa ctcaaacagc ttggcatcgg cgtttcgcgc cgggcggggg aaaaaaccga
    14281 agaataggcc cggacagccg gttttccggc tgcgggcttt tgttttcaga cggcatttgg
    14341 tgcaaatgcc gtctgaaatc gtaaggggac ggattttatg acagaggacg aacgtttcgc
    14401 gtggctgcaa ttggcgttta cgccctatat cggcgcggaa agcttcctgc tgctgctgcg
    14461 ccgtttcggc agcgcgtcaa acgccctgtc cgcaccggcg gaacaggtgg cggcactgat
    14521 acggcacaaa caggcgcttg aggcttggcg caatgcggaa aaacgcgctc tggcgcggca
    14581 ggcggcagaa gcggcattgg aatgggaaat gcgggacgga tgccgcctga tgctgcttca
    14641 ggatgaagat ttccccgaaa tgctgacgca ggggctgacc gcgccgccgg ttttgttttt
    14701 gcgcggcaac gtgcggctgc tgcacaagcc ttccgccgcc atcgtcggca gccgccatgc
    14761 cacgccgcag gcaatgcgga ttgccaaaga tttcggcaag tcgttgggcg ggcaaaatat
    14821 ccccgttgtg tcgggtatgg cttccggtat cgatactgcc gcccatcagg gcgcactgga
    14881 ggcagaaggc ggcaccatcg ccgtgtgggg gacgggtata gaccgcattt acccgccgtc
    14941 caataaaaac cttgcctatg aaatcgccga aaaaggattg attgtcagcg agttccccat
    15001 cggcacgcgg ccgtatgccg gcaattttcc gcgccgcaac cgcctgattg ccgccctgtc
    15061 gcaagtaacg ctggtggttg aagccgcctt ggaatccggt tcgctgatta ccgccggcct
    15121 ggcggcagaa atggggcgcg aagtgatggc ggtacccggt tcgatagaca gtccacacag
    15181 caaaggctgc cacaaactga ttaaagacgg cgcaaaattg gtggaatgcc tggacgacat
    15241 cctgaacgaa tgcccggggc tattgcaaaa tacgggtgct tcatcatatt ctataaataa
    15301 ggatacgccc gacacgggac gccgcgccgt tcagacggca tacgccccgc cgcctgccgc
    15361 aaaaatgccg tctgaagcgg cggcaggcgg cacagcccac ggcggcatac tggataaaat
    15421 gggtttcgac cccgtccatc ccgacgtgct tgccgagcag cttgctatgc ctgccgcaga
    15481 tttgtatgcc gcactgttgg aattggaatt ggacggcagc gttgccgcaa tgcccggcgg
    15541 cagataccag cgtatccgaa cttgaacgca ctttatatta aggaacacga atgaccgaag
    15601 tcatcgccta cctcatcgaa catttccaag atttcgatac ctgtccgccg cccgaagact
    15661 tgggtatgct gcttgaagaa gcgggtttcg atacgatgga aatcggcaac accctgatga
    15721 tgatggaagt attgctcaac agctccgaat tttccgccga acctgccgac agcggcgcat
    15781 tgcgcgtgta cagcaaagaa gaaaccgaca acctgccgca ggaagtgatg gggctgatgc
    15841 agtatctgat tgaagaaaaa gccgtcagct gcgaacagcg ggaaatcatc atccacgcgc
    15901 tcatgcacat tccgggcgac gaaattaccg tagataccgc caaagtgctg accctgctgc
    15961 ttttatgggc aaacaagagc gagctgcccg tgttggtcgg cgacgagctg atgagcgcgc
    16021 ttttactcga caacaaaccc acgatgaact gaagcggctt cagacggccc gcccgaggcc
    16081 gtctgaaacg ccggcatcaa aaccaccatc cagagaacga caaatggcga aaaacctatt
    16141 aatcgtcgaa tccccgtcca aagccaaaac cctgaaaaaa tatttgggcg gcgattttga
    16201 aatccttgca tcctacggac acgtccgcga cctcgtcccc aaaagcggcg cggtcgatcc
    16261 cgacaacggc tttgcgatga aataccaatt gattagccgc aacagcaaac acgtcgatgc
    16321 catcgttgcc ggtgccaaag aagctgaaaa catctacctc gccaccgacc cggataggga
    16381 aggcgaagcc atttcctggc atcttttgga aatcctcaaa tccaaacgcg gcttgaaaaa
    16441 catcaagccg cagcgtgtcg tgttccacga aatcaccaaa aacgccgtgc tcgatgccgt
    16501 tgcccatccg cgcgaaatcg aaatggactt ggtcgatgca caacaagccc gccgtgcttt
    16561 ggactatttg gtcggcttta acctttcgcc attgttatgg aaaaaaatcc gccgaggttt
    16621 gagcgcgggg cgtgtgcaaa gccctgcttt gcgcctgatt tgtgagcgcg aaaacgaaat
    16681 ccgcgcgttt gaagcgcagg aatattggac ggtgcatctc gacagccaca aaggccgcag
    16741 caagttcacc gccaaactcg cccaatacaa cggcgcgaaa ctcgaacaat tcgacctgtc
    16801 gaacgaagcc gcgcaagccg acgtattgaa agaactcgaa ggcaaagaag ccgtcgttac
    16861 cgccattgaa aagaaaaagc gcagccgcaa tcctgccgcg ccgttcacca cttctaccat
    16921 gcagcaggat gccgtgcgca aactcggctt caccaccgac cgcaccatgc gtaccgccca
    16981 acagctttat gaaggtatag acgtagggca gggcgcgatc ggtctgatta cctatatgcg
    17041 taccgacagc gtgaatttgg cggatgaagc gttaaccgaa atccgccatt acattgaaaa
    17101 caaaatcggc aaagaatatc tgccgagtgc cgccaaacaa tacaaaacca aatccaaaaa
    17161 cgcccaagaa gcccacgaag ccatccgtcc gacttccgtg taccgcacgc ccgaaagcgt
    17221 caaacccttc ctgagcgcag accagttcaa actctatcaa atgatttggc agcgtaccgt
    17281 cgcctgtcag atgacgcctg ccaaattcga ccaaaccacc gtcgatatta ccgtcggcaa
    17341 aggcgtattc cgcgtaaccg gacaagtgca aaccttcgca ggcttcttga gcgtttatga
    17401 agaaagcagc gacgatgaag aaggcgaaga cagcaaaaaa ctgcccgaaa tgagcgaagg
    17461 cgataaattg cccgtggaca aactctacgg cgaacaacac tttaccactc cgccgccgcg
    17521 ctacaacgaa gccacgctgg ttaaagccct cgaagaatac ggcatcggcc gcccctcgac
    17581 ctacgccagc atcatctcca cgctcaaaga ccgcgaatac gttacccttg agcaaaaacg
    17641 cttcatgccc accgacacag gcgacatcgt caacaaattc ctgaccgaac acttcgccca
    17701 atacgtcgat taccacttca ccgccaaact cgaagaccag cttgacgaaa ttgccgacgg
    17761 caaacgccaa tggattccct tgatggacaa attctggaaa ccgttcatca aacaagtgga
    17821 agaaaaagaa ggcatcgaac gcgccaaatt taccacgcag gaacttgatg aaacctgccc
    17881 gaaatgcggc gaacacaaac tgcaaatcaa attcggcaaa atgggtcgtt ttgttgcgtg
    17941 tgccggttat cccgagtgca gctacacgcg caacgtcaac gaaactgccg aagaagccgc
    18001 cgagcgcatc gccaaagccg aagccgaaca ggcagaactc gacggacgcg aatgtcctaa
    18061 atgtggtggt cgcctagtgt acaaatacag ccgaaccggc agcaaattca tcggctgcac
    18121 caactacccc aaatgcaaac acgtcgagcc gctggaaaaa ccgaaagata ccggcgtcca
    18181 gtgtccgcaa tgcaaaaaag gcaacctcgt cgagcgcaaa tcccgctacg gcaaactgtt
    18241 ttacagttgc agcacctatc ccgactgtaa ctacgccaca tggaacccac ccgttgcaga
    18301 aagctgtcca aaatgcgcat ggccggtttt gaccatcaaa accaccaaac gctggggtgt
    18361 agaaaaagtc tgcccacaaa aagaatgcgg ctggaaagaa cagattgaac cgcccgcgcc
    18421 gaaggagtaa gattaggttg gtttgaaaga gaaaaggtcg tctgaaaaat tttcagacga
    18481 cctttgcttt tctgtgattg gtttatttga atccgcgtgt tgttttaaag tccgataaaa
    18541 tccggttcat ttcaggcgca aacaaggcga tgtaatcgta agatagaccg cgactggcac
    18601 tgggatgggg aaagcagacg acttcgcaat cttcaaacga ttggaatttg acattgaaac
    18661 gtgtaccgtc aaattctttt tgcaccgtct ccagcggttt ggtctgctta ccgaccaact
    18721 gctcgaagcg tggcagtaca ttttggttgt tcagaaaatc cgccaacctg ctgcccatga
    18781 agaggatgac tttcggacgc agtttttcga tgtggtagag aaaattatcg atgtgctcgg
    18841 gttgtgtgaa cttgtctgga ttgtcgatag tgttgccctg tgtagcagcc cagttggttt
    18901 gaaccaggga tttttcaaat gcaccgccca atccattttc gtctaagggg tgtccccaca
    18961 tttcaaacca attttttatc gtattatcgt aacgccactt ttttgcctgc tctccgaaat
    19021 agagggattt gtttgcaaat gtatggtcga ttttgttttc agggagtttg tattcacctg
    19081 ctacataagc agcctcatcg gctttactcc aaccccattc atagccacaa atcattaagc
    19141 catgtttgtc gttgtagcct ttgaacaggc tgttgctcaa attcaaatcc ttcatcatga
    19201 actcttcctt ttaaaattta agagcgattg acttcaatgt ttttagatgg ggtggaaaaa
    19261 tccttgtgta ggcaacataa attcaataaa tttcttgata attcgaaacc tactaatagc
    19321 gcacctataa aagctttttc attacgttca gcatgacggt cacgtcgttc atatttttta
    19381 cgcttgctgt tccctgttat tacagctaag ccaagtgata tggcgagaat tgcccaaaca
    19441 attgtactta ataacaattt tccccatact atcaataagg aaagaaaaaa ccttttagta
    19501 ttagatcgat aggttataat ccatgcccat gaaaatgcaa taagagttat acataagata
    19561 caagctgaca ggatttcttt atttttaatt aaataactta gagcgatgag gatgacaggt
    19621 gtcaagaaaa taatagttac atcccgatag ctataaaaga aaactgccct attttgaatg
    19681 tggagatgtg cacagaatcc aatatagcta aggataatag ttaatataat aaaaaaagac
    19741 caccaagggt gaagagatag gaattccatg ttttcccttt ttttgtaaaa aggaaaaaat
    19801 cttatctaat agttaattgc ctaaccagcc agaagaagtt taaaatctat cccaataatt
    19861 caaccatcta tacagaaagt tcagcttatg gaaacccacg aaaaaatccg cctgatgcgc
    19921 gaattgaata aatggtccca ggaggatatg gcggaaaagc tggcgatgtc ggcaggcggg
    19981 tatgccaaaa tcgaacgagg cgaaacgcag ttgaatatcc cgcgtttgga gcagttggcg
    20041 cagattttca aaattgatat gtgggacttg ctcaaatcgg gcggcggcgg gatggtgttg
    20101 cagattaacg atgtggatac caacagcggg gaatttgcaa tctataccgc tcaggatgca
    20161 tcggtaaagc tggatttgtt aaaaatggaa ttaaaacact gtaaagaaat gttggaacac
    20221 aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac agatatgccg
    20281 tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata ccgtttgaac
    20341 ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagtaacc gggtacgcat
    20401 tatcggcggg caatgccggg gcaggaaatt aagtttcaca tccgccgacg gactgcgtcc
    20461 gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt tgacgggtaa
    20521 aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg cttcgcgcaa
    20581 cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct tgcagaaaaa
    20641 cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtctgt tcagacggca tcgcatattt
    20701 gaagaccgta tccgaacagt ttgatgttgt ctttcttgac ccgccgtttg catggcagga
    20761 ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccggtcat tcgtctatct
    20821 cgaggcgggt acgctgccgg atattcccga ttggctgacg gaatatagag aggggaaatc
    20881 ggggcagagt acatttgaat taagggtttt ccaagtggct gaataatatg cgctttgata
    20941 atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa ccttgtgcta
    21001 taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat gtcgcttttt
    21061 attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa tgatgccatc
    21121 tcgcaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg cgtcggacac
    21181 tacgatgaac cgcagtgcca gcaggtttgc ccggtggact gcatcctgat tgacgaagaa
    21241 catcccgaaa cgcatgacga gttgatggcg aaatacgaaa agattatcca gtttaaataa
    21301 attcttcttt aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac
    21361 ggaaaagcaa gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa
    21421 gggggcagac tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc
    21481 aaaattctta cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag
    21541 ccttccaagc ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat
    21601 gtagctcagg ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca
    21661 tgggcaccat ccctcgatta ttcatttctt taaggcttag atatatagga tattgccatg
    21721 gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt
    21781 gaccatggta aaaccaccct gactgccgct ttgactacta ttttggctaa aaaattcggc
    21841 ggtgctgcaa aagcttacga ccaaatcgac aacgcacccg aagaaaaagc acgcggtatt
    21901 accattaaca cctcgcacgt ggaatacgaa accgaaaccc gccactacgc acacgtagac
    21961 tgcccggggc acgccgacta cgttaaaaac atgattaccg gtgccgcaca aatggacggt
    22021 gcaatcttgg tatgttccgc agccgacggc cctatgccgc aaacccgcga acacatcctg
    22081 ctggcccgcc aagtaggcgt accttacatc atcgtgttca tgaacaaatg cgacatggtc
    22141 gacgatgccg agctgttgga actggttgaa atggaaatcc gcgacctgct gtccagctac
    22201 gacttccccg gcgacgactg cccgatcgta caaggttccg cactgaaagc cttggaaggc
    22261 gatgccgctt acgaagagaa aatcttcgaa ttggctgctg cattggacag ctacatcccg
    22321 actcccgagc gtgccgtgga caaacctttc ttgttgccta tcgaagacgt attctctatt
    22381 tccggtcgtg gtacagtagt aaccggccgt gtagagcgcg gtatcatcca cgttggtgac
    22441 gagattgaaa tcgtcggtct gaaagaaacc caaaaaacca cttgtaccgg tgttgaaatg
    22501 ttccgcaaac tgctggacga aggtcaggcg ggcgacaacg taggcgtatt gctgcgcggt
    22561 accaaacgtg aagacgtgga acgcggtcag gtattggcca aaccgggtac tatcactcct
    22621 cacaccaagt tcaaagcaga agtgtacgta ttgagcaaag aagagggtgg ccgccatacc
    22681 ccgttcttcg ccaactaccg tccgcaattc tacttccgta ccaccgacgt aaccggcgcg
    22741 gttactttgg aagaaggtgt ggaaatggta atgccgggtg agaacgtaac cattaccgta
    22801 gaactgattg cgcctatcgc tatggaagaa ggcctgcgct ttgcgattcg cgaaggcggc
    22861 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
    22921 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
    22981 gccaaataaa aaattaaccg gccttgtgtc ggttaatttt tttgtatttg ttatttagta
    23041 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
    23101 gacagaacat acgcctgaaa aaaagaacgt taaagtggat caactggttg ttcaagataa
    23161 agaatctgca tctaattccg gtaaggaagg gttttttgca tatttctcaa attcttggtc
    23221 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgct gtcagaatga ctgtatttgt
    23281 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
    23341 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
    23401 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgcattgccc
    23461 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
    23521 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgctagttg
    23581 agatggaaat gacagatgac tcttggcatc ttgtaaaaag caccccccgt gtttccggtt
    23641 ttattggagg gagggctaat agacctacgc cgattagtca gagagaggct gaaattattt
    23701 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
    23761 aacaggttcg tgtaaatgaa gggccgtttg cggattttaa cggggtggtt gaggaggtca
    23821 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
    23881 agctggagtt cagccaggtt gaaaagatta actgattttt atacttgaaa aaaaagcaat
    23941 aagaggatag aatcaaaaat taacttgggg agcggaaatg gttccgcgtc ttacccgttt
    24001 ttaggagtcc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
    24061 ggtaaagcca atccatctcc tccggttggt cctgctttgg gtcagcgcgg tttgaatatt
    24121 atggaatttt gtaaggcatt taatgctgca acccaaggta tggagcctgg cttaccgatt
    24181 ccagttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aaccccgcca
    24241 gcttctatct tgttgaaaaa ggctgctggt ctgcaaaaag gtagttctaa tcctctgacc
    24301 aacaaagtgg gtaaattgac ccgtgctcag ttggaagaaa ttgctaaaac taaagagcct
    24361 gatttgactg ctgctgactt ggatgcggct gtccgtacta tagcaggttc tgctcgctca
    24421 atgggcttgg atgtagaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagctc
    24481 ttcgctcttc tgtggaagcc aataaattat atgcaattga tgaagcaatt gctttggtaa
    24541 aaaaagcagc gactgctaaa tttgacgagt ctgttgacgt atctttcaac ttgggcgttg
    24601 atccgcgtaa atctgaccaa gttatccgtg gttcggtcgt tctgcctaaa ggcaccggta
    24661 agacaacccg tgtggctgta tttgctcaag gtgcaaatgc agaagctgct aaagaagctg
    24721 gtgcagatat cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
    24781 ttgacgttgt tattgcttct cccgatgcaa tgcgtattgt tggtcaattg ggtaccatct
    24841 tgggccctcg tggtctgatg ccaaacccta aagtaggtac ggttacccct aatgttgccg
    24901 aagcagtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaaa gcaggtattg
    24961 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
    25021 cgttgctgga tgctatcgtt aaagccaagc ctgctgccgc taaaggtcag tatctgaaaa
    25081 aagttgctgt gtccagcacc atgggtttgg gtattcgcgt tgatacatca agcgtaaata
    25141 actaatctta aggaatgttc aagcagtttg gttttctggg ctgcttgaat ttgggctact
    25201 taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta actgccctac
    25261 gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt ttaggttacc
    25321 gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta gaccttgagt
    25381 ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat tgctaatgct
    25441 caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac tgagcttcgt
    25501 gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatacttt ggctcgtcgt
    25561 gcagtgcaag gtacttcatt tgcagaattg gccgatcaaa tggttggtcc gttggtttac
    25621 gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc gaaaaaagat
    25681 gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc tgctcaggtt
    25741 gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaagctgtt gttcgttatg
    25801 caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc
    25861 gaagaagccg cttaatcgat tttgtttctg ttaatcaatt attttttaat acaatatttg
    25921 gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg ttctttgacc
    25981 gtaatggaat tgaacgactt ggttaaagct tttgaagaaa aatttggtgt ttctgctgct
    26041 gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa
    26101 tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt
    26161 gcaattaccg gtttgggtct gaaagaagct aaagacatcg ttgacggcgc acctaaaacc
    26221 attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact ggaagaagca
    26281 ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca gttttctgcc
    26341 agccttattt tgcttcttaa aataaacatc aagtattgtt tacatttatt tgcacagttt
    26401 ttatcaagtc attgcaaata aatgtaaata tcagattgat gcgtgccgtt gctttcagac
    26461 ggcctattat tgaaaattac ttttcggagt gtatatgagc tattcgttta ccgagaaaaa
    26521 acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt tcctgctggc
    26581 aacccaaatt gattcttatg cgaaattttt gcaactggaa aatgcttttg acaaacgtac
    26641 cgatgacggt ctgcaggcag catttaattc tattttcccg attgtgagcc ataacggtta
    26701 tgcgcgattg gagtttgtgc attacacatt gggcgagcct ttgttcgata ttcccgaatg
    26761 tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg cgtatccgtt tggtgatttt
    26821 ggataaggaa gcatctaaac cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg
    26881 cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag agcgtgtgat
    26941 tgtttcccag ttgcaccgtt cgcccggcgt gtttttcgag catgacaaag gtaagacgca
    27001 ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt catggttgga
    27061 ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc
    27121 ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg atattttcta
    27181 cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa accgatttgg tcgcaggccg
    27241 tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg tattggttgc
    27301 caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag gcctgacccg
    27361 tttggatgta gaaccagaaa gcctgctggg caaagcattg gctgccgatc tgattgattc
    27421 ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt tgttggccaa
    27481 atttgatatc aacggcgtaa aagaaattac gaccctttac atcaatgagt tggatcaggg
    27541 tgcttatatc tccaatacct tgcgtacgga tgagactgcc ggccggcagg cggctcgtgt
    27601 tgcgatttac cgtatgatgc gtccgggcga accgcccact gaagaggcgg tcgagcaatt
    27661 gtttaaccgc ttgttcttca gtgaagacag ctacgatttg tcccgcgtag gccgtatgaa
    27721 atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact cttggtacgg
    27781 tcgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt
    27841 cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt
    27901 ggacgatatc gatcacttgg gcaaccgccg agtacgttcg gtaggcgagc tgactgaaaa
    27961 ccaattccgt agcggtttgg cccgtgtgga acgtgccgta aaagaacgtt tgaatcaggc
    28021 ggaatcagaa aacttgatgc cgcacgattt gattaatgca aaacctgttt ctgccgctat
    28081 taaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca atcccttgtc
    28141 tgaagtaacc cataaacgcc gcgtatccgc attgggtccg ggcggtttga cccgcgaacg
    28201 tgcaggattt gaggtgcggg acgtgcatcc gacccactac ggtcgcgtat gtccgattga
    28261 aacgcctgaa ggtccgaaca tcggtttgat caactcattg tccgtttatg cgcgcaccaa
    28321 tgattacggt ttcttggaaa cgccttaccg ccgcgttatc gacggcaaag taaccgagga
    28381 aatcgattac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg cgaatgccga
    28441 tttggattca gatggcaatc tgattggcga tttggttacc tgtcgtgaaa aaggcgaaac
    28501 cattatggca acgcccgacc gcgtccaata tatggacgtg gcaactggtc aagtggtatc
    28561 cgttgcagca tccctgattc cattcttgga acatgatgac gcgaaccgcg cattgatggg
    28621 tgccaacatg caacgtcagg cagtgccttg cttgcgtcct gaaaaaccga tggtcggtac
    28681 cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc gccgaggcgg
    28741 cgtggtcgag tatgtcgatg ccaaccgcgt tgtgatccgt gtccatgacg acgaagcgac
    28801 tgccggtgaa gtgggtgtcg atatttacaa cttggttaaa ttcacccgtt ccaaccagtc
    28861 taccaatatc aatcagcgtc ctgccgtcaa agccggcgat gttttgcaac gcggcgattt
    28921 ggtggccgac ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa atatgaccat
    28981 cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt ccgaaaaagt
    29041 ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga
    29101 tactaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg agcgtatgca
    29161 aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag ccggcgatgt
    29221 gttggtaggc aaggtaacgc ctaaaggcga aacccaactg acgccggaag aaaaactgct
    29281 gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat acttcattgc gtatgcctac
    29341 cggcatgagc ggtaccgtta tcgacgttca agtcttcact cgtgaaggta ttcaacgcga
    29401 caaacgcgcc caatccatta tcgattccga gttgaaacgc taccgtttgg atttgaacga
    29461 ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gaacgtatga ttgtgggtca
    29521 aaaagccaat ggtggtccga tgaagctggc taaaggcagc gaaattacta ccgaatattt
    29581 ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ctgaccgatg aagatttggc
    29641 caagcagttg gaactgatta aagtgagcct gcaacaaaaa cgcgaagaag cggacgagtt
    29701 atacgaaatc aagaagaaaa aactgaccca aggcgacgaa ttgcaacccg gcgtacaaaa
    29761 aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa gccggcgaca aaatggcggg
    29821 ccgccacggt aacaaaggcg tggtatcgcg cattctgcca gtggaagaca tgccttacat
    29881 ggcggacggc cgtccggtag acatcgtact gaacccattg ggcgtacctt cccgtatgaa
    29941 catcggtcag attttggaag ttcacttggg ttgggcagca aaaggtatcg gcgagcgtat
    30001 cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg cgcgagttct tgaacaaact
    30061 ctacaacggc agcggcaaga aagaagattt ggatgccctg actgatgaag aaatcatcga
    30121 actggcctcc aacctgcgta aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa
    30181 agagtctgaa atccgcgaaa tgttgaactt ggcttatccg agcgacgatc ctgaggttga
    30241 aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg tatgacggcc gttcaggcga
    30301 agcatttgac cgcaaggtta cagtaggtgt gatgcactat ctgaaactgc accacttggt
    30361 tgacgaaaaa atgcacgcgc gttctaccgg tccgtacagt ctggttaccc agcagccttt
    30421 gggcggtaaa gcccagttcg gcggccaacg tttcggcgag atggaggttt gggcattgga
    30481 agcatacggc gcggcataca cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa
    30541 cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat
    30601 gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggcttgg atatcgattt
    30661 ggaacgttac taaacaaaag ttttcagacg gcctttcagg gtcgtctgaa aaagtggttt
    30721 cagaataaga atgaagcaat cggcatttag gccgtctgaa atcaaaagta ccgtttccca
    30781 atatcgaaaa tccgccatgc ggtaaaaata cttccttcaa ggagcaaaaa tgaatttgtt
    30841 gaacttattt aatccgttgc aaactgccgg catggaagaa gagtttgatg ccattaaaat
    30901 cggtattgcc tctcccgaaa ccatccgctc atggtcttat ggcgaagtca aaaaacctga
    30961 aaccatcaac taccgtacgt tcaaacctga gcgtgacggt ttgttctgtg ccaaaatctt
    31021 tggcccggtc aaagactacg aatgcttgtg cggaaaatac aaacgcttga aatttaaagg
    31081 cgtaacgtgt gaaaaatgcg gcgtggaagt aaccctgtcc aaagtgcgcc gcgaacgcat
    31141 gggtcatatc gaattggctg cgcccgtcgc acatatttgg ttcttaaaat ccctgccttc
    31201 ccgcttgggt atggtgttag acatgacttt gcgcgacatc gagcgcgtat tgtactttga
    31261 agcatttgtg gtaaccgatc ccggtatgac tccgctgcaa cgccgccaat tgctgactga
    31321 agacgattac tacaacaagc tggacgaata cggcgacgat ttcgatgcca aaatgggtgc
    31381 ggaaggtatc cgcgaattgc tgcgtaccct gaatgtagcg ggcgaaatcg aaatcctgcg
    31441 ccaagagttg gaatcgaccg gttccgacac caaaatcaaa aaaatcgcca aacgcttgaa
    31501 agtattggaa gctttccatc gttccggtat gaaactggaa tggatgatta tggatgtgct
    31561 gccggtattg ccgcctgatt tgcgtccgtt ggttccattg gatggtggtc gttttgccac
    31621 ttccgatttg aacgatttgt accgccgcgt tattaaccgt aacaaccgtc tgaaacgtct
    31681 gttggaactg catgctcctg acatcatcgt ccgcaacgaa aaacgtatgt tgcaagaagc
    31741 agttgactcg ctgttggata acggccgtcg cggtaaagcc atgaccggcg ccaacaaacg
    31801 cccgctgaaa tcattggcag acatgattaa aggtaagggt ggccgcttcc gtcaaaacct
    31861 gctgggcaaa cgtgtggact actccggccg ttccgtgatt accgtaggcc cgtacctgcg
    31921 tctgcaccaa tgcggtttgc cgaaaaaaat ggctttggaa ctgttcaaac cgttcatttt
    31981 ccacaaattg gaaaaacaag gtttggcctc taccgttaaa gcagcgaaaa aattggtaga
    32041 gcaagaagta ccggaagtat gggacatctt ggaagaagtc atccgcgaac atccgattat
    32101 gctgaaccgt gcgccgaccc tgcaccgttt gggtattcaa gcgttcgaac ctatcttgat
    32161 tgaaggtaaa gcgattcagt tgcacccatt ggtgtgtgct gcgttcaacg ccgactttga
    32221 cggcgaccaa atggcggtac acgttccatt gagcttggaa gcacaaatgg aagcacgcac
    32281 gctgatgctg gcttcaaaca acgtattgtc tcctgccaac ggcgaaccga ttatcgtacc
    32341 ttcccaagac atcgtattgg gtctgtacta catgacccgc gaccgtatca atgccaaagg
    32401 cgagggcagc ctgtttgccg atgtgaaaga agtgcatcgc gcataccata ccaaacaggt
    32461 tgaactgggt acgaaaatta ccgtacgtct gcgcgaatgg gtgaaaaacg aagcaggtga
    32521 gtttgagcct gtcgttaacc gttacgaaac aaccgtcggc cgtgcattgt tgagcgaaat
    32581 cctgccgaaa ggcctgccgt ttgaatatgt caacaaggca ttgaagaaaa aagaaatttc
    32641 taaactgatt aacgcatcgt tccgcctgtg cggcttgcgc gatacggtta tctttgctga
    32701 ccacctgatg tacaccggtt tcggatttgc ggcaaaaggc ggtatttcca ttgccgttga
    32761 cgatatggaa attccaaaag aaaaagcggc cttgctggct gaagccaatg ccgaggttaa
    32821 agaaatcgaa gaccaatacc gtcaaggttt ggttaccaac ggcgaacgct acaacaaagt
    32881 ggtggatatt tggggtcgtg ccggcgataa aatcgctaaa gcgatgatgg acaacctgtc
    32941 caaacagaaa gttattgacc gtgacggcaa cgaagtcgat caagagtcat tcaactccat
    33001 ctatatgatg gctgactccg gtgctcgtgg ttctgcggct cagatcaaac agttgtccgg
    33061 tatgcgcggt ttgatggcga aacctgacgg atcgattatt gaaacgccga ttacctcaaa
    33121 cttccgcgaa ggtctgaccg tattgcaata ctttattgcg acccacggtg cgcgtaaggg
    33181 tttggcggat accgcattga aaaccgcgaa ctccggttac ctgacccgtc gtctggtaga
    33241 cgtaactcaa gatttggtcg ttgttgaaga cgattgcggt acttcagacg gctttgttat
    33301 gaaggcagtg gtacaaggcg gtgatgtgat tgaagcattg cgcgatcgta ttttgggccg
    33361 tgttactgcg tctgacgttg tcgatccgtc aagcggcgag accttggttg aagccggtac
    33421 gttgttgact gaaaaactgg tggatatgat tgaccaatcc ggtgtcgatg aagtcaaagt
    33481 ccgtacgccg attacttgta aaacccgcca cggtttgtgc gcgcactgtt atggtcgcga
    33541 cttggcacgt ggtaagctgg tcaatgccgg tgaggcagtt ggtgtgattg ccgcccaatc
    33601 aatcggcgaa ccgggtaccc agctgactat gcgtacgttc cacatcggtg gtgcggcatc
    33661 ccgtgcggca gcagccagcc aagtggaagc caaatccaac ggtacggcac gattcagcag
    33721 ccaaatgcgt tatgttgcca acaataaagg cgagttggtt gtcatcggcc gttcttgtga
    33781 agtcgtgatt cacgacgata tcggccgtga acgcgaacgc cacaaagtac cttacggtgc
    33841 catcctgctg gtacaagacg gtatggccat taaagccggt caaaccttgg caacctggga
    33901 tccgcatacc cgtccgatga ttaccgaaca cgcaggtatg gtgaaattcg aaaacgtgga
    33961 agagggcgtt accgttgcca aacaaaccga tgatgtaacc ggtttgtcca ctttggtggt
    34021 gattgacggt aaacgtcgtt cctctagtgc ttccaaactg ctgcgtccga ctgtgaaact
    34081 cttggacgaa aacggcgtgg aaatctgtat tcccggtact tctactccgg tatccatggc
    34141 attccccgtt ggtgcggtga ttaccgtacg cgaaggtcag gaaatcggta aaggcgacgt
    34201 attggcgcgt attccgcaag cctcttccaa aacccgcgac attaccggcg gtctgccgcg
    34261 cgttgccgaa ttgtttgaag cacgcgtgcc gaaagatgcc ggtatgttgg cggaaattac
    34321 cggtaccgtt tccttcggca aagagaccaa aggcaagcaa cgtctgattg ttactgacgt
    34381 ggacggtgta gcatacgaga ccttgatttc caaagagaaa caaattctgg tacacgacgg
    34441 tcaagtggta aaccgcggtg aaaccatcgt ggacggagct gttgatccgc atgatattct
    34501 gcgtttgcaa ggtatcgaag cactggcacg ctacattgtc caagaggtgc aagaggttta
    34561 ccgtctgcaa ggtgtgaaga tttctgataa acacatcgaa gtcatcatcc gtcaaatgtt
    34621 gcgccgtgtg aacattgcgg atgccggcga aaccgggttc attaccggag agcaggtcga
    34681 acgcggcgat gtgatggctg ccaatgaaaa agcgttggaa gaaggcaaag aaccggcgcg
    34741 ttacgaaaac gtattgctgg gtattaccaa agcttccctg tccaccgaca gcttcatttc
    34801 tgccgcatcg ttccaagaaa ccacgcgcgt cctgaccgaa gccgcgatta tgggcaaaca
    34861 agacgagttg cgtggtttga aagaaaacgt catcgtcggt cgcttgattc ctgccggtac
    34921 cggtttgact taccaccgca gccgtcatca acaatggcaa gaggtggaac aggagactgc
    34981 tgaaacccaa gtaacggatg aataatcttt ggtgcgttta ttcaatacaa aaccgcaagc
    35041 tttaagcttg cggtttttct ttgtctgatt aaggcaaaaa caagcgtttt cgtcattttg
    35101 aggcgtgtgg attattcctt aggtattttc gggccggaga ccaacgaggt ggcgggtgtc
    35161 gtcggtacgt ccggagacca aaataacttt gccagggatg ttggtttcgg cggtcaaaaa
    35221 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    35281 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    35341 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    35401 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    35461 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    35521 tgaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    35581 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    35641 cccgcctttt attcgcgcag cagcaaggaa tggtttgtca gtatagaaaa aacgtattga
    35701 cagtattttc tccagccgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa
    35761 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    35821 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    35881 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    35941 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    36001 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    36061 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    36121 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    36181 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatacg gtgctaagcg
    36241 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagctga acaacggccg
    36301 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    36361 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    36421 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    36481 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    36541 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    36601 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    36661 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    36721 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    36781 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    36841 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    36901 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctatactgg
    36961 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    37021 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    37081 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    37141 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    37201 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    37261 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    37321 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    37381 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    37441 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    37501 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    37561 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    37621 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    37681 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    37741 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    37801 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    37861 acgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    37921 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    37981 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    38041 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgcatg gaattccccg agccggtaat
    38101 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    38161 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    38221 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    38281 attcggtgtg gaagcaaata tcggtgcacc tcaagtggct taccgtgaaa ctatccgcaa
    38341 agccgttaaa gctgaataca aacacgcgaa acaatccggt ggtaaaggtc aatatggtca
    38401 cgttgtgatt gaaatggaac ctatggaacc ggggggcgaa ggttacgagt ttatcgatga
    38461 aattaaaggt ggcgtgattc ctcgtgaatt cattccgtct gtcgataaag gtatccgcga
    38521 tacattgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtttggt
    38581 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    38641 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    38701 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    38761 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    38821 acctctggca gaaatgttcg gttattcgac cgacctgcgt tctgcaaccc aaggccgcgc
    38881 tacttactct atggagttca agaaatattc tgaagctcct gcccacatag ctgctgctgt
    38941 aactgaagcc cgtaaaggct aatcaggcaa ataggtcgtc tgaaaggctg aaatgatttt
    39001 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagctca tggctaagga
    39061 aaaattcgaa cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg
    39121 taaaaccacc ctgactgccg ctttgactac tattttggct aaaaaattcg gcggtgctgc
    39181 aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa
    39241 cacctcgcac gtagaatacg aaaccgaaac ccgccactac gcacacgtag actgcccggg
    39301 ccacgccgac tacgttaaaa acatgattac cggcgccgca caaatggacg gtgcaatcct
    39361 ggtatgttcc gctgctgacg gccctatgcc gcaaacccgc gaacacatcc tgctggctcg
    39421 ccaagtaggc gtaccttaca ttatcgtgtt catgaacaaa tgcgacatgg tcgacgatgc
    39481 cgagctgttg gaactggttg aaatggaaat ccgcgacctg ctgtccagct acgacttccc
    39541 cggcgatgac tgcccgattg tacaaggttc cgcactgaaa gccttggaag gcgatgccgc
    39601 ttacgaagaa aaaatcttcg aactggctgc cgcattggac agctacatcc cgactcccga
    39661 gcgtgccgtg gacaaaccgt tcctgctgcc tatcgaagac gtgttctcca tttccggccg
    39721 cggtacagta gtaaccggcc gtgtagagcg cggtatcatc cacgttggtg acgagattga
    39781 aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc ggtgttgaaa tgttccgcaa
    39841 actgctggac gaaggtcagg cgggcgacaa cgtaggcgta ttgctgcgcg gtaccaaacg
    39901 tgaagacgtg gaacgcggtc aggtattggc taaaccgggt actatcactc ctcacaccaa
    39961 attcaaagca gaagtatacg tactgagcaa agaagagggt ggtcgtcaca ctccgttctt
    40021 cgccaactac cgtccgcaat tctacttccg taccaccgac gtaaccggcg cggttacttt
    40081 ggaagaaggt gtggaaatgg taatgccggg tgaaaacgta accatcaccg tagaactgat
    40141 tgcgcctatc gctatggaag aaggcctgcg ctttgcgatt cgcgaaggcg gccgtaccgt
    40201 gggtgccggc gtggtttctt ctgttatcgc ttaattgaag gatattgata aatggcaaac
    40261 caaaaaatcc gtatccgcct gaaagcttat gattacgccc tgattgaccg ttctgcacaa
    40321 gaaatcgttg aaactgcaaa acgtactggt gcagttgtaa aaggcccgat tcctttgccg
    40381 accaaaatcg agcgtttcaa cattttgcgt tctccgcacg tgaacaaaac ttcccgtgag
    40441 caattggaaa tccgcaccca cttgcgcctg atggacatcg tggattggac cgataaaact
    40501 accgatgcgc tgatgaagct ggatttgccg gccggtgttg atgtagaaat caaagtccaa
    40561 taattcggac tattgaaaat ccccaagcaa tcaatgcttg gggatttttt atgttatgcc
    40621 gagacctttg caaaattccc caaaatcccc taaattccca ccaagacatt taggggattt
    40681 ctcatgagca ccttcttcca gcaaaccgcc caagccatga ttgccaaaca catcgaccgg
    40741 ttcccactat tgaagttgga ccgggtaatt gattggcagc cgatcgaaca gtacctgaat
    40801 cgtcaaagaa cccgttacct tagagaccac cgcggccgtc ccgcctatcc cctgttgtcc
    40861 atgttcaaag ccgtcctgct cggacaatgg cacagcctct ccgatcccga actcgagcac
    40921 agcctcatca cccgcatcga tttcaacctg ttttgccgct ttgacgaact gagcatcccc
    40981 gattacagtc atcaaccata ttccggtttg tcggagaaag atgcatacgc tgtgatgacc
    41041 ggataccgac cctttaaaag agtccgaccc tatgccgtct gaaaattcaa aacgcttcag
    41101 acggcatatt gaagatattt ctgatatttc tgttgatatt tctttaactt gtcagatata
    41161 atgccgagct tggtacattt gtgccaagtt taactttgtc tgaaagacag gccaatcgta
    41221 gcctgtccct ttactttaaa aggaaaataa tcatgacttt aggtctggtt ggacgcaaag
    41281 ttggtatgac ccgcgtgttc gacgaacagg gtgtttctgt tccggtaacc gttttggata
    41341 tgtctgccaa ccgcgttaca caagtaaaat ccaaagatac tgacggctat actgccgttc
    41401 aagttacctt tggtcagaaa aaagccaatc gtgtcaacaa agccgaagcc gggcactttg
    41461 caaaagcagg tgttgaagcc ggtcgcggtt tgattgagtt tgctttgact gaagaaaaac
    41521 tggctgaatt gaaagctggt gacgaaatca ccgtttctat gtttgaagtc ggtcaactgg
    41581 tcgatgtaac cggtatctct aaaggtaaag gtttctccgg cacgattaaa cgtcataact
    41641 tcggtgccca acgtacttcc cacggtaact cccgttctca ccgcgttcca ggttctatcg
    41701 gtatggcgca agacccgggt cgcgtgttcc ccggtaaacg catggccggc caatacggca
    41761 acaccaaagc aactgttcaa aaattggaag ttgtccgtgt tgatgcagaa cgccaactgc
    41821 tgttggttaa gggtgctgtt ccgggtgcgg tcaacagcga tgttgtagtt cgtcccagcg
    41881 tgaaagtagg tgcgtaatgg aattgaaagt aattgacgct aaaggacaag tttcaggcag
    41941 cctgtctgtt tctgatgctt tgttcgcccg cgaatacaat gaagcgttgg ttcatcagct
    42001 ggtaaatgcc tacttggcaa acgcccgctc tggtaaccgt gctcaaaaaa cccgtgccga
    42061 agtaaaacac tcaaccaaaa aaccatggcg tcaaaaaggt accggccgcg cccgttccgg
    42121 tatgacttca tctccgctgt ggcgtaaagg tggtcgcgcg ttcccgaaca aacccgacga
    42181 aaacttcact caaaaagtaa accgcaaaat gtaccgtgcc ggtatggcga ctattctgtc
    42241 ccaattgact cgtgacgagc gtttgtttgc gattgaggcg ttgactgccg aaactcctaa
    42301 aaccaaagtt tttgctgaac aagtgaaaaa tctgggtctg gagcaagtgt tgtttgtaac
    42361 caaacagctc gacgagaatg tttacttggc ttcacgcaac ttgccaaacg tgttggtttt
    42421 ggaagctcaa caagttgatc cttacagctt gctgcgttac aaaaaagtaa tcatcactaa
    42481 agatgcagtt gcacaattag aggagcaatg ggtatgaatc aacaacgttt gactcaagtg
    42541 attttggcac ctatcgtttc tgaaaaaagc aacgtattgg ctgaaaaacg caaccaaatg
    42601 acgtttaaag tcttggcaag tgcaaccaaa cctgaaatca aagcggctgt cgagctgctg
    42661 tttggtgttc aagttgcttc tgtaactacc gttacaacta aaggcaaaac taagcgtttt
    42721 ggtcgtactt tgggccgccg ccgcgatgtt aaaaaagctt atgtaagctt ggctgccggt
    42781 caagagttgg atttggaagc cgctgctgca gctgcagata aggaataaac aaaatggcaa
    42841 tcgttaaaat gaagccgacc tctgcaggcc gtcgcggcat ggttcgcgtg gtaacagaag
    42901 gtttgcacaa aggtgcgcct tatgcacctt tgctcgaaaa gaaaaattct actgctggtc
    42961 gtaacagtaa tggtcatatc accacccgtc acaaaggcgg cggtcataaa caccattacc
    43021 gtgttgtaga ctttaaacgt aacaaagacg gcattcctgc caaagtagag cgtatcgaat
    43081 acgatcctaa ccgtactgcc ttcatcgcac tgttgtgcta tgcagacggc gagcgtcgtt
    43141 acatcattgc tcctcgcggt attcaagccg gtgccgtatt ggtttccggt gctgaagctg
    43201 ccattaaagt aggcaacacc ctgccgattc gcaacattcc cgttggtacg actatccact
    43261 gtatcgaaat gaaacccggt aaaggtgctc aaatcgcacg ttctgccggt gcttctgcgg
    43321 tattgctggc taaagaaggc gcgtacgctc aagtccgcct gcgctctggc gaagtccgta
    43381 aaatcaacgt aaattgccgt gcgaccatcg gtgaagttgg taacgaagag caaagcctga
    43441 aaaaaatcgg taaagccggt gccaaccgtt ggcgcggtat tcgtccgacc gtacgcggtg
    43501 ttgtcatgaa tcctgtcgat cacccgcatg gtggtggtga aggccgtacg ggcgaggccc
    43561 gcgaaccggt cagcccatgg ggtactcctg ctaaaggcta ccgcactcgt aataacaaac
    43621 gcacggataa catgattgtt cgtcgccgtt actcaaataa aggttaattt agtatggctc
    43681 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgctc
    43741 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
    43801 attttatcgg tctgaccatt gctgtgcaca acggccgcac ccatgtgcct gtgtttatca
    43861 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
    43921 acttggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
    43981 taaaaatgcc cgtatctccg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
    44041 agacgttgcc caagctttga atattttggc tttcagtcct aaaaaaggtg ccgagctgat
    44101 taaaaaagta ttggagtcag ctattgctaa tgccgagcac aataacggtg cggacattga
    44161 tgaactgaaa gtggtaacta tctttgttga caaaggccca agcttgaaac gttttcaagc
    44221 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
    44281 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
    44341 aactaaagac tgggcttcaa aatggtttgc taaaagcacc gacttttcta ctgttttgaa
    44401 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
    44461 agtggttatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
    44521 tgtggttatc ggtaaaaaag gtgaggatat cgaggttttg aaacgtgact tgcaagtctt
    44581 gatgggtgta cctgttcatg taaatattga agagattcgc cgtcctgagt tggatgctca
    44641 aattattgct gacggtattg cccagcagtt ggaaaagcgc gttcaattcc gtcgtgctat
    44701 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
    44761 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
    44821 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
    44881 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
    44941 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
    45001 accgtaagca acaaaagggt cgcaatactg gcatcgctac tcgcggtaat aaggtaagtt
    45061 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
    45121 ctgctcgtcg tgcaatgacc cgtcatatca aacgtggtgg tcgtatttgg attcgtgtat
    45181 tccctgataa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
    45241 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
    45301 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
    45361 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
    45421 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
    45481 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
    45541 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
    45601 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
    45661 gttgagcgta aagtaaaaca tccgctgtat ggtaagatta ttcggttatc tactaaaatc
    45721 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
    45781 cgtccattgt caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct
    45841 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
    45901 tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg aagtttcttc cctttcgggg
    45961 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
    46021 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataacatg attcaaatgc
    46081 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
    46141 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
    46201 atgcggctcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
    46261 ctgctaaggg tgtacgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
    46321 tgttactgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
    46381 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
    46441 tggcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
    46501 aggtaagcag ggtcaagtag ttcgagtgtt gggtgataaa gttgttgttg agggcgttaa
    46561 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attgagggcg gtattattac
    46621 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
    46681 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
    46741 caaatcaaat ggctctatca ttggggcata aggagataac atggctcggt tgagagagtt
    46801 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
    46861 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
    46921 aaaagttatg gaacatgctg tttccgattt agagaaaatt gccggtcaaa aaccggttgt
    46981 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
    47041 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
    47101 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
    47161 ttacaatatg ggtgttcgtg agcaaattat ttttccggaa attgaatacg ataaaattga
    47221 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
    47281 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
    47341 attaatcgta atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
    47401 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
    47461 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
    47521 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
    47581 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
    47641 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
    47701 gctaataaag cagcggttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
    47761 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
    47821 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
    47881 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
    47941 ggtattgcta ttgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
    48001 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
    48061 cgcaaaaaac ccagtgactg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
    48121 agttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
    48181 atttaatgat ggcaaattgg cttttgttgc gaataacagc agtaaacaag caaatgcaat
    48241 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
    48301 gaaaagattg caattgatag gtgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
    48361 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
    48421 aactcctagc caaacagaga ttgttttaac cggctcggat aaacaagttg ttggtcaagt
    48481 tgctgctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
    48541 tgtaggagaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
    48601 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
    48661 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
    48721 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
    48781 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
    48841 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
    48901 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
    48961 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
    49021 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
    49081 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
    49141 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
    49201 taaaaaatgg tacgattcat catgaggtta ttggtcgaca tggtgctact aaagtattta
    49261 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
    49321 atgctatggg cattcataat atctccgcca aagtgcacgg atctactaac ccatataata
    49381 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
    49441 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
    49501 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
    49561 ggtttaggtt tgcgtcgtcg cgagcatacg gtagaggttt tagatacccc tgaaaaccgt
    49621 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
    49681 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
    49741 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
    49801 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
    49861 ttttaaatct ttaacagcat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
    49921 tgctgttaat gagattgata ttttggtctt aaagcaagcg ggtctgattg catctacagt
    49981 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
    50041 taaagttacc aaaggtgcga gagctgctat cgaggctgtt ggtggtaaga ttgaaatgta
    50101 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaaatt tggagatctt
    50161 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    50221 cccgtacctg gagttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    50281 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    50341 ataggaatta tgccatatat ttcagcttct attattgtac agcttgcttc tgaaattttg
    50401 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    50461 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    50521 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    50581 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    50641 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    50701 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgtttatt
    50761 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    50821 attcctattc attatgcaaa acgccagttt aatggtaggg cgggtagtca aaatacgcat
    50881 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    50941 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    51001 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    51061 gttattttct tttgttattt ttatacggct ttggttttta gccctaaaga aatggcagag
    51121 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    51181 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    51241 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    51301 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    51361 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    51421 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    51481 taatgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    51541 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    51601 accttatgat ctaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    51661 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    51721 cgaaatcgtg tagttcgtgt aatttgtact gatctccgtc acaaacagcg tcaaggttaa
    51781 tggaatattt cttttaatgt gattctgtga tatagtgaca cactttgccc taaaaaggaa
    51841 aaaatatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    51901 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    51961 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgacc
    52021 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga ggtaactatg agtatcaagc
    52081 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    52141 gtcaacgcac tcgtacaaat gcgcgtaccc gcaaaggtcc gcgtaaagcg attgctggta
    52201 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    52261 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    52321 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    52381 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    52441 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    52501 ggtccaggtc gtgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    52561 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    52621 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    52681 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    52741 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    52801 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    52861 tcgttatttc gcagaagctg atcgtcgtaa aggttctacc ggcgagttgc tgttgcagtt
    52921 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    52981 agcaagacag cttgtttctc ataaggcgat agttgtgaat ggacaagttg tcaatattcc
    53041 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    53101 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    53161 agatgcggat aaacttgagg gtgtgttcaa aaacatgccg gatcgctcgg aattgaccgg
    53221 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    53281 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    53341 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    53401 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    53461 aagtagctat tgccggtgta ttacacgaat attctactgt tgatggtatt caggaagatg
    53521 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    53581 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    53641 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    53701 aaattgagat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    53761 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    53821 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    53881 ataagttggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    53941 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtacgcctg
    54001 tggaggaggt tgaagaaaaa gcacctccta tcgaccctgt tcttttgcgt ccggtggatg
    54061 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    54121 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    54181 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    54241 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    54301 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    54361 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    54421 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    54481 aaaccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    54541 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgggtgt tgaaatacgg
    54601 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    54661 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    54721 atctgatagg taatagggta ttggctatca tgtttaaaat attaattgaa tagctaaggt
    54781 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgctttc
    54841 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacgtc agacttgttt
    54901 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    54961 tttgtgctgg atgttcaaga gtttgattat cccgagtctt tggatcttgc tgcattggtt
    55021 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    55081 gctgctgcgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    55141 gaactgggtc aggttgaggt gcagaacacg gaggatggtc agaaagcaag gaaaacagta
    55201 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    55261 gttacggggg cggtcagcca gggggcggaa ttgattgcgg atggcaatat acatatttat
    55321 gcgccgatga gggggcgtgc tttggccggt gccaagggtg atacttctgc ccgcatattt
    55381 atccactcca tgcaggcaga actggtttct gtggcgggta tttaccgtaa ttttgaacag
    55441 gatttgccga accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    55501 gttatcagtg caattggctc agagtaattg tttgatattt aaaaaggaaa tattgtggca
    55561 aaaattattg tagtaacttc aggtaagggc ggtgtcggta aaacgactac cagtgccagt
    55621 attgcgacag gtttggcatt acgcggatat aaaactgcgg taattgattt tgatgtgggt
    55681 ttgcgtaacc tcgacctcat tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat
    55741 gtcattcagg gggaggcgac gctcaaccag gctttgatta aagataaaaa ttgtgaaaac
    55801 ctgtttattt tgccggcttc ccagactcgg gataaagacg ctttgacacg cgagggcgta
    55861 gaaaaagtga tgcaggagct gtccggcaag aaaatgggct ttgagtatat tatttgcgac
    55921 tctcctgccg gtattgagca gggtgcattg atggcgttgt attttgctga tgaagccatt
    55981 gtaacgacca atcctgaggt ttccagtgtg cgtgactccg acaggatttt gggaattttg
    56041 caaagcaaat cccataaggc agagcaaggc ggttcggtta aagaacatct gttgattacg
    56101 cgttattctc ccgaacgtgt ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat
    56161 attctgcata ttcctttgct gggtgtgatt cctgaatccc aaaacgtctt gcaggcatcc
    56221 aattccggag aaccggtcat ccatcaggac agcgtggcgg cttccgaggc atataaggac
    56281 gttattgccc gtcttttggg cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa
    56341 agcttcttca aacgtctgtt tggaggataa ggtatgtcat taatcgaatt tttattcggc
    56401 agaaagcaga aaacggcaac cgttgcccgc gaccgccttc aaatcatcat tgcccaagag
    56461 cgcgcccaag aaggtcaggc tccggattac ctgccgactt tacgtaaaga gttgatggaa
    56521 gtcctgtcca aatatgtgaa cgtttcatta gacaatatcc gtatttccca ggaaaaacag
    56581 gatggtatgg atgtgcttga gttgaacatt actttgccgg aacagaaaaa ggtataggac
    56641 atgaccttaa ccgaattgcg gtacatcgtc gcagtcgccc aagaacgtca tttcggcagg
    56701 gcggcgcggc gttgttttgt cagccagccc actttgtcta ttgccattaa gaaattggaa
    56761 gaagagcttg ccgtctcttt gtttgaccgg agcagcaacg atattattac gaccgaggcg
    56821 ggggaacgta tcgttgcaca ggcgcgtaag gtgttggaag aggcagagct tatcaggcat
    56881 ttggcaaatg aagaacaaaa cgagctggag ggtgcgttca aactcgggct gatttttacg
    56941 gttgcgccgt acctgctgcc gaaactgatt gtctcgttgc gccgtactgc accgaaaatg
    57001 cctttgatgt tggaagagaa ttacacgcat actttgaccg agtcgctcaa acgcggggac
    57061 gttgacgcga ttatcgttgc cgaaccgttt caagagccgg gcattgttac cgaacccttg
    57121 tatgacgaac cgtttttcgt gattgtcccg aaagggcatt catttgagga actggatgcc
    57181 gtttcgccac ggatgctggg tgaggagcag gttttgctgc tgacggaagg caactgtatg
    57241 cgggatcagg tactctcaag ctgttccgaa ttggcggcga aacaacgtat acaggggttg
    57301 accaatacat tgcagggcag ctcgattaat acaatccgcc atatggttgc cagcggtttg
    57361 gcaatcagcg tgttaccggc aaccgcgctg accgaaaacg atcatatgct gttcagcatt
    57421 attccgtttg aaggtacgcc gccaagccgg cgggtcgtat tggcgtaccg ccgcaatttt
    57481 gtccgtccga aggcgttgtc ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg
    57541 gtaagtttta tccgcgacta ggcgcaggca ttgttttcaa aacgccattt ccctgagccg
    57601 acaacacggt atgccaagat attgccgtca tcatcgattt tgagtatagc atcgccacgg
    57661 aaactgccgt cctgaagata ttcgactttt gcatcactgt gaatgttttc atcagtgccg
    57721 atgcaatgcc atgtatagtg gattaacaaa aaccagtacg gcgttgcctc gccttgccgt
    57781 actattgcca aggcgtacca gcctgatttg ttgatgtgtg tactactgca tgggttgtta
    57841 agccctgcaa gtgatttaag tgtgaagatt gagtaatgcc cactagtcgc aggcgcgggg
    57901 gcaggtgcta agtcattggt tatctcctta ctatctatct aactacaggg tgtattatgc
    57961 gcctccttgt tgttttgtgc aagtggtttt ctgataattt atgtaaagat ttggtaaaga
    58021 cgtatagcct ttttctttgc attgtgcttg cgcagggtat caggtaggtc tcaaaaaatt
    58081 tgaaaatttt tatgttttcg tataaaatct tttgcactta tacggagtga gttatgcggt
    58141 acaccattaa aacatttcaa acccttgctg atgaaacttt cccctctaag aacttcaaaa
    58201 ttcttttgtt caatggggtg gcacgaccta taaccattgt ttgccctaca cacggggtgc
    58261 agactgtttc tgcggggcaa gcatttatcc gttccaaaca cggctgtcct gcctgtggtt
    58321 tagttttatc aacctccgtt ttggcctcta ggggtcggtc agtctccatc ctcgacaccg
    58381 ccacaggcga aacactctcg ttccccagcg tacaagctgc cgccaaagcc ctaaacacct
    58441 cctacggctc tatacgaacc aagctcgacg gacgttcgtc ccccgacaac cttgtctgta
    58501 acaggtacaa agtaatgccg taatctcgaa acttcgtgct ataataaact cctagctttt
    58561 gcaggagttt gttttttatg gatttaaatg tcgaaatcaa gaattttggc aaggttgccc
    58621 atgctagggt ttccattcgc ccgcttaccg tcatcacggg ttgcaactcc acactcgcgt
    58681 tccccagcgt tactgccgca ggcactgctt tgggtgtgca ttttcagcaa attaaccaca
    58741 ggctcaaggg tcgaacgtcc tgtcaggagg tgttgatttc tacaccgatt atactgtctg
    58801 cggcttcgtc gccttgtcct gatttttgtt aatccactat aaaagaggcc gtctgaaaaa
    58861 catttttcag acggccttgt ttattcaatc aaatcagtct ttcaacttcg ccaactgatt
    58921 ttgaactttt gccattttgt cttccaattc cgccaaatcg gctttgtctt tttccaccag
    58981 atgcgcaggg gctttttcgg tgtagccggg tttggagagt ttggcgttga gtttgtccaa
    59041 ggctttttgc agcttctcgg cttctttgtt caagcgggcg gtttcggcgg ctttgtcgat
    59101 ttcgactttc aacatcaggc gcgcgccgtt gcagacggcg acgggcgcgt cttcgctttc
    59161 gggtagggcg gcgacttgct gtgcttcggt caggcgggtc atcatcggca ggtatttgag
    59221 gtagtccgcc aagtcgtccg tgctttcgac aaacagcggg gcttttacgt tgggctggat
    59281 gcccatttcg ccgcgcaggt tgcggactgc gccaatcaaa tcctgcaaca cggtcatttg
    59341 ctcgaatgcc gtctgaacaa tctcgccgct gtcggcttcg gggaagcggg cgagcatgat
    59401 gctgtcggcg gttttcgcgt cgcacatagg agcgacggtt tgccacagtt cttcggtaat
    59461 gaacggaata atcgggtgca gcaggcgtag ggcggcttcg agtacgcgca ataaggtatg
    59521 gcgtgtggcg cgctggcggc tggcgcagcc ggtttgaagc tgcactttgg cgagttccaa
    59581 ataccagtcg caatagtcgt tccatacgaa gctgtacagg gtttccgctg acaaatcaaa
    59641 gcgataggtt tcgtaggctt gcgtaacctg ttcgatggtc tgattcagac ggcctataat
    59701 ccacatatcg gggaaggagt agccgcgcgg ttcggcagcg gttgcgccgt aaccgcagtc
    59761 ttggttttcg gtattcatca agacgaagtt ggtggcgttc cagattttat tgcagaagtt
    59821 gcggtagcct tcggcgcgtt tgaagtcgaa gttcacgctg cgccccaagc tggcgtagct
    59881 cgccatggtg aaacgcaatg cgtccgcgcc catactcgga atgccttcgg gaaagagttt
    59941 tttggtggct tcttccactt tcggcgcggt ttcaggtttg cgcaggccgg tggtacgttt
    60001 taccagcagc ttgtccaagc cgatgccgtc aatcaaatcc acaggatcga tgacgttgcc
    60061 ttcggatttg gacatttttt tgccttcgtg gtcgcgcacg atgccgtgga tgtacacgtc
    60121 tttaaacggt actttgccgg taaagtgggt ggtcatcata atcatgcgcg ccacccagaa
    60181 gaagatgatt tcgtagccgg ttaccaagac gttggacggc aggaaggctt tgagttcgtc
    60241 ggtttcagac ggccagccga gcgtggaaaa cggcacgagc gcggaggaga accaagtgtc
    60301 caatacgtct tcttcgcggg tcaagcctgt tttgccggct tgtttttcgg cttcagcctg
    60361 attgcgggcg acgtacacat tgcctgtttc gtcgtaccac gcggggattt gatgccccca
    60421 ccacagttgg cgcgaaatac accagtcttg gatgttgttc atccattggt tgtaggtatt
    60481 gacccagttt tcagggataa agcgtaccgc gccgctgtcg acggcttttt tggctttgtc
    60541 agcaaggctt aagcctttga attcgctgtc aggctcgccg ccgtttggcg tggcggacat
    60601 ggcgacaaac cattggctgg tcagcattgg ctcaatcact gagccggttc ggtcgccttt
    60661 gggtgtcatc agcgtgtgtg ctttgatttc gaccaacaag ccttgttcct gcaaatcggc
    60721 aaccatttgt ttgcgcgcgg caaagcggtc taaacctgcg tatttttcag gcagggcaaa
    60781 gccttgttgc gcttcgcctt tgaagttgaa cacttcggcg tttgccagca ctttggcttc
    60841 caagtcgaac acattaatca ggcgcgtgtc gtggcgtttg ccgacttcgt agtcgttgaa
    60901 gtcgtgtgca ggcgtgattt tcacgcagcc tgtgccgaag tctttttcaa cgtatttgtc
    60961 ggcaatcacg gggatggtac ggccggtcag cggcaggatt aattctttgc cgattaagtg
    61021 ggtgtaacgt tcgtcttcgg gattgacggc tacggcaacg tcgcccagca acgtttcggg
    61081 acgggtggtc gccacgataa cggcttcggc aggattgtcc gccagcggat agcggatgtg
    61141 ccacatagag ccttgttctt ccacgctttc tacttccaaa tcagacaccg ccgtgccaag
    61201 caccggatcc cagttcacca agcgtttgcc gcgataaatc aagccctgtt catacaggcg
    61261 tacgaacact tcggttacgg tttcggcgcg tacatcgtcc attgtgaaat actcgcgcgt
    61321 ccagtcggca gaacagccca cacggcgcat ctgctgcgta atcgtgccgc cggaaacttc
    61381 tttccattcc cacacttttt ccaagaattt ttcacgaccc aagtcatgac gggacacgtt
    61441 ttgcgcagca agctgacgct cgaccacgat ttgcgtggcg atgcccgcgt ggtcggtgcc
    61501 gggaatccag gcggtattgc agcctttcat gcggtagtag cgggtcaggc cgtccataat
    61561 ggtttggttg aaggcatggc ccatgtgcag cgtgccggtt acattgggcg gcggcagttg
    61621 gatggaaaaa gacggtttgg tcaaatccat atcgggctgg aaatagcctt ggctttccca
    61681 gttttgatag tgtttggatt cgatttcggc gggggagtat ttgtctaaca tgatggaact
    61741 ttgtgaaatt aaggttattt ttgatgtgcg gattataacg caaaaaggcc gtctgaatca
    61801 tttcagacgg cctttggcat acaggtttta aaaatggaac aataccaggc tgacggcaat
    61861 caccgccatg cccattgtca ggccgtaaac ggtttcatgg ccgtctgaat agcgtttggc
    61921 agccggcagc agctcgtcca acgccaaaaa caccatcaca ccggctatca cgccgaatac
    61981 cgaaccaaac acggcaggcg acaaaaacgg ctgcaaaacc aaatagccca aagccgcccc
    62041 caacggctcg gccaagccgg atagcagaca cgcccacacc gttttcttac ggctgcgggt
    62101 ggcaaaataa accggcgcgg cgatggaaat gccctccgga atattatgga tggcaatcgc
    62161 caaggccaaa ggcatcccga ctgctggatt ttccaatgtg gcaaaaaacg tcgccaagcc
    62221 ttcggggaaa ttgtgcgcag taatcgcaaa cgccgccatc atgccgactc gcgcgatatg
    62281 gcggcgtttg ctttcttgaa acgacgggtc ttgcgcgtct aaagtttcat gcgggttcgg
    62341 caccagacgg tcaatcagcg caatgccgcc catccctgcc aaaaatgcca tggtcgccgc
    62401 cgcaaacgcg tggtctttat cataaatttc agcgaacgcc tcgctggact tactgaaaat
    62461 ctccgtcagg gaaacataca ccatcgcacc gccggcaaat gccaaaccaa acgacaacac
    62521 gcgcggattg ggcgttttgg aaaacatcac caagccgctg cctaatacgg taaacaaacc
    62581 ggcagccaac gtaatggaaa aggcaacggc caaattggac atcgaaaaat cgggcatggg
    62641 aaaacctgcg ctaaaagctg ggacaggttc agactaacac tttttaatgc gtatgataat
    62701 agttattatt tattttattg attggatacc cgaattttga aacaaaaggc cgtctgaaaa
    62761 atgattttca gacggccttt aaatttgaaa tgtcgctaaa ccttatcgct ttccagctta
    62821 agcctgatag cgcgacaggc tcaaatcgtc gcttcggatt tcggtgtctt tgccgctcac
    62881 gatatcggcg gttaattttg ccgaacccag cgacatggtc cagcccaaag tgccgtggcc
    62941 tgtattcaag aataagtttt caaagcgggt gcggccgatt aacggcgtgc tgtcgggcgt
    63001 catcggtctg aggccgctcc agaacaatgc ttggctcaaa tcgccgcctt ccgggaacaa
    63061 atcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttga tttcgtagcc
    63121 cgacaattct gccatgccgc cgacgcggat tctgttgtca aagcgcgtga tagcgacttt
    63181 gtagctttca tccaaaacgg tggacaccgg cgcgccgtct gaattggtga ccggcagggt
    63241 caaggaatag cctttgacgg gataaatcgg cagatcgagg tctaactgcg ccaacacagt
    63301 cctactgaag cagccgagcg cacagacaac ggcatctgct tcaaaccgcc ctgtttcggt
    63361 ttcaacggct ttgatgcgca gcccgttatg gtcgatgcgg ctgatggttt ggttgaaatg
    63421 aaactgtacg cccttttcct gacacaattt gtataggttt tcagtgaaga ggtggcagtc
    63481 gccggtcgca tctgcaggca ggtgcaggcc gccggcaatt ttggcggtaa cgcgtgccag
    63541 cgcaggctca aattctgcac attcttcggg tttcagacgg cggtacggca cgccgtagcg
    63601 ttccaaaacg gcaatgtctt gttctgccgc ttcgacttct ttggtttggc ggaaaatctg
    63661 caacgtccct tttttgcgtt cctcaaaatt catgccggtt tgcgcttcaa aacggcggaa
    63721 catttcacgg ctatattcgg aaatcctgac catgcgctct ttattggttt gatagtgcgc
    63781 tgccgtgcag ttttgcagca tttgccacag ccattcgatt tgatacaggc tgccgtcggg
    63841 gcgaaacagc aaaggcggat ggcttttaaa cagccatttc agcgctttgg tcgggatacc
    63901 gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcgttgg caaaactggt
    63961 ttccatcgcc acaccctcgg cgcggtcgat gaccgttact tcatgtcctg cctctgccag
    64021 ataccacgcg gaagacacgc cggcaacacc cgcacctaaa acaagcactt tcatgtttct
    64081 ccctccggct ttttcaaaac agacttaata tgccgtgccg tctgaatatt cggattcaga
    64141 cggcctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga agtcgcgcgt
    64201 ttcattgacg atgactttcg ccatcaataa aagtgcaatc aggttgggca atgccatcaa
    64261 gccgttgaat gtgtccgaag ccagccacac caaatcaagg ctcaacacgg tacccagcat
    64321 aacggaagaa acataaccga cgcggtacaa accggcaaat ttctcgccga aaacatacac
    64381 cgcgcatttt tcgccgtaat agcaccagcc gagaatggtt gagtaggcaa agaaaatcag
    64441 gccgatggtg acaatccagc cgccgatgcc gggcagcatt ttttggaatg tgacggttgt
    64501 cagtgccgcg ccgctcactt caggtttgac aaactcgccg cccgcgccga gcagtcccat
    64561 taccaacacg atgccggtaa tcgagcaaac gacgatggta tccaaaaacg taccggtcat
    64621 agaaaccaag gcctgacgga cgggatggtc ggttttcgcg gctgcggcgg caataggcgc
    64681 agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc ggatgaccgt
    64741 accgatagcg ccgcccgcca ctgcctgcgc gctgaacgca tcggagaaaa ttagcttgac
    64801 ggcaggcatc agtgcatcgg aattaatcgc gataatggaa agaccgccca acacataaaa
    64861 caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa taccacctaa
    64921 aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt cgataccgaa
    64981 gctggtttgc accgcctgtg caaccgagtt ggactgcacc gagctgccga taccgaagga
    65041 agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt tgcccaagcc
    65101 tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt tgacgcggta
    65161 tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatgg cggtcatcca
    65221 catccaaaat accgcgcccg ggccgccggt taccaccgca gtcgccacgc cggcgatgtt
    65281 acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg aaatatcgcc
    65341 ttcgtggcct tcgccgcctt catacttctt tgacggcaca aacgcctgtt tcagcgcata
    65401 gcccaacata gtgaactgta agcctttcag cataacggtc agcacaatgc cggtgccgac
    65461 cagcagcatc aacataacag atccccagac ccagccgctg acggtttcga aaaaggcttt
    65521 gagattgtct aaaaacaatt gcatggcttt ctcctttgtc t
//
LOCUS       DAXIEB010000002        59552 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000002 DAXIEB010000000
VERSION     DAXIEB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 59552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.002"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..297)
                     /locus_tag="ACFOA3_000082"
     CDS             complement(<1..297)
                     /locus_tag="ACFOA3_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6897166.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFTDYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNHS"
     gene            complement(453..1046)
                     /gene="ribA"
                     /locus_tag="ACFOA3_000083"
     CDS             complement(453..1046)
                     /gene="ribA"
                     /locus_tag="ACFOA3_000083"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6897167.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(1039..1716)
                     /locus_tag="ACFOA3_000084"
     CDS             complement(1039..1716)
                     /locus_tag="ACFOA3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897168.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            1870..1954
                     /locus_tag="ACFOA3_000085"
     tRNA            1870..1954
                     /locus_tag="ACFOA3_000085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1904..1906,aa:Leu,seq:tag)
     gene            2475..3509
                     /gene="purM"
                     /locus_tag="ACFOA3_000086"
     CDS             2475..3509
                     /gene="purM"
                     /locus_tag="ACFOA3_000086"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6897169.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(3983..4639)
                     /locus_tag="ACFOA3_000087"
     CDS             complement(3983..4639)
                     /locus_tag="ACFOA3_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6897170.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(4636..6408)
                     /locus_tag="ACFOA3_000088"
     CDS             complement(4636..6408)
                     /locus_tag="ACFOA3_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6897171.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            6614..7228
                     /locus_tag="ACFOA3_000089"
     CDS             6614..7228
                     /locus_tag="ACFOA3_000089"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6897172.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            7391..8008
                     /locus_tag="ACFOA3_000090"
     CDS             7391..8008
                     /locus_tag="ACFOA3_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6897173.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            8016..8879
                     /locus_tag="ACFOA3_000091"
     CDS             8016..8879
                     /locus_tag="ACFOA3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6897174.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(8892..9326)
                     /locus_tag="ACFOA3_000092"
     CDS             complement(8892..9326)
                     /locus_tag="ACFOA3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6897175.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            9472..10155
                     /gene="rpe"
                     /locus_tag="ACFOA3_000093"
     CDS             9472..10155
                     /gene="rpe"
                     /locus_tag="ACFOA3_000093"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6897176.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPQTLEKIRQV
                     RAMLDCYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RVELAKAAG"
     gene            complement(10222..11253)
                     /gene="ruvB"
                     /locus_tag="ACFOA3_000094"
     CDS             complement(10222..11253)
                     /gene="ruvB"
                     /locus_tag="ACFOA3_000094"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6897177.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHTLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(11335..11643)
                     /locus_tag="ACFOA3_000095"
     CDS             complement(11335..11643)
                     /locus_tag="ACFOA3_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897178.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            complement(11866..13119)
                     /locus_tag="ACFOA3_000096"
     CDS             complement(11866..13119)
                     /locus_tag="ACFOA3_000096"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6897179.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLHECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQVDSGKIAAECWAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            complement(13191..14819)
                     /locus_tag="ACFOA3_000097"
     CDS             complement(13191..14819)
                     /locus_tag="ACFOA3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6897180.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTTGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            14969..15343
                     /locus_tag="ACFOA3_000098"
     CDS             14969..15343
                     /locus_tag="ACFOA3_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6897181.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTVEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            15403..16941
                     /locus_tag="ACFOA3_000099"
     CDS             15403..16941
                     /locus_tag="ACFOA3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6897182.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGVDYIVQVGD
                     EKISEQSLNAAVQNEQAGGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPTNEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            17008..17625
                     /gene="recR"
                     /locus_tag="ACFOA3_000100"
     CDS             17008..17625
                     /gene="recR"
                     /locus_tag="ACFOA3_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6897183.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(17681..17959)
                     /locus_tag="ACFOA3_000101"
     CDS             complement(17681..17959)
                     /locus_tag="ACFOA3_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897184.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            18165..19412
                     /locus_tag="ACFOA3_000102"
     CDS             18165..19412
                     /locus_tag="ACFOA3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6897185.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            19405..20100
                     /gene="lolD"
                     /locus_tag="ACFOA3_000103"
     CDS             19405..20100
                     /gene="lolD"
                     /locus_tag="ACFOA3_000103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6897186.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEILAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGTLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSRHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGCFGRVMVM
                     KDGSLHPKQGANA"
     gene            20167..21912
                     /gene="recD"
                     /locus_tag="ACFOA3_000104"
     CDS             20167..21912
                     /gene="recD"
                     /locus_tag="ACFOA3_000104"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6897187.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTILDRETYKSLNLYLPEHGFPVHEDAPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNAQVERLYRAHKAYWQAVKDGN
                     IEAAYVGISDIVVLAAWRQDSEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(21984..23087)
                     /locus_tag="ACFOA3_000105"
     CDS             complement(21984..23087)
                     /locus_tag="ACFOA3_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6897188.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAQAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPRAVACARANIARLGLEKQVEIRETDMFPDMRADLIVCNPPWLPAKPTSAVESALY
                     DPDSAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPIHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(23062..23238)
                     /locus_tag="ACFOA3_000106"
     CDS             complement(23062..23238)
                     /locus_tag="ACFOA3_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6897189.1"
                     /translation="MPSETFWLQTAWAWKDKAGTKNCSVLPLLAVPSVWFTVLPPYFQ
                     NMTQVSHVFFYFSR"
     gene            23338..25800
                     /gene="lon"
                     /locus_tag="ACFOA3_000107"
     CDS             23338..25800
                     /gene="lon"
                     /locus_tag="ACFOA3_000107"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6897190.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGNDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGVEAAAESASKPKPRSRATKH"
     gene            25982..26251
                     /locus_tag="ACFOA3_000108"
     CDS             25982..26251
                     /locus_tag="ACFOA3_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6897191.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(26310..26744)
                     /locus_tag="ACFOA3_000109"
     CDS             complement(26310..26744)
                     /locus_tag="ACFOA3_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897192.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(26737..28044)
                     /locus_tag="ACFOA3_000110"
     CDS             complement(26737..28044)
                     /locus_tag="ACFOA3_000110"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6897193.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            28242..28613
                     /locus_tag="ACFOA3_000111"
     CDS             28242..28613
                     /locus_tag="ACFOA3_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6897194.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLARQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIGVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            28690..30612
                     /locus_tag="ACFOA3_000112"
     CDS             28690..30612
                     /locus_tag="ACFOA3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6897195.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            31078..31593
                     /locus_tag="ACFOA3_000113"
     CDS             31078..31593
                     /locus_tag="ACFOA3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897196.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(31883..32008)
                     /locus_tag="ACFOA3_000114"
     CDS             complement(31883..32008)
                     /locus_tag="ACFOA3_000114"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897197.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(32159..33115)
                     /locus_tag="ACFOA3_000115"
     CDS             complement(32159..33115)
                     /locus_tag="ACFOA3_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6897198.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAVIQPGSRPGRLIRKWSE
                     SLLDAFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAA
                     NLTVVFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERL
                     SQELENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMT
                     QEKAAKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQ
                     QLISRLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            complement(33185..33301)
                     /locus_tag="ACFOA3_000116"
                     /pseudo
     CDS             complement(33185..33301)
                     /locus_tag="ACFOA3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(33433..34806)
                     /locus_tag="ACFOA3_000117"
     CDS             complement(33433..34806)
                     /locus_tag="ACFOA3_000117"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6897199.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKDYVLTLLFLKYVSDKHKQGGGMIELPAGTTFDDIVKLKNTADIGDR
                     LNKIIAQIAEANDLKGVIDVADFNDEDKLGKAKEMIDRLSRLVGIFEKLNLSSNQAED
                     DDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSKIIGISADCPSSTSVYDPTCGSGSL
                     LLKAAAQAGSQISLYGQEKDVATASLARMKMILHNNETAEINTGTPCPIRLSVMKTTG
                     LRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAIILPHGVLFRGNAEARIRTE
                     LLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQFAEEGTNQVISGGSVFMID
                     ASRGFIKDGNKNRLREQDIHKIIDTFTKQEEIPRYSRMVHLSEIAAQDYNLNLPRYID
                     SGEVEDL"
     gene            complement(34803..35462)
                     /gene="ung"
                     /locus_tag="ACFOA3_000118"
     CDS             complement(34803..35462)
                     /gene="ung"
                     /locus_tag="ACFOA3_000118"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6897200.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            35543..35950
                     /locus_tag="ACFOA3_000119"
     CDS             35543..35950
                     /locus_tag="ACFOA3_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6897201.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            35965..36912
                     /locus_tag="ACFOA3_000120"
     CDS             35965..36912
                     /locus_tag="ACFOA3_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6897202.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(37019..38089)
                     /locus_tag="ACFOA3_000121"
     CDS             complement(37019..38089)
                     /locus_tag="ACFOA3_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6897203.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            38274..38549
                     /locus_tag="ACFOA3_000122"
     CDS             38274..38549
                     /locus_tag="ACFOA3_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6897204.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            38551..39174
                     /gene="lipB"
                     /locus_tag="ACFOA3_000123"
     CDS             38551..39174
                     /gene="lipB"
                     /locus_tag="ACFOA3_000123"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6897205.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            39167..40135
                     /gene="lipA"
                     /locus_tag="ACFOA3_000124"
     CDS             39167..40135
                     /gene="lipA"
                     /locus_tag="ACFOA3_000124"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6897206.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECEF"
     gene            40198..41940
                     /locus_tag="ACFOA3_000125"
     CDS             40198..41940
                     /locus_tag="ACFOA3_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897207.1"
                     /translation="MDQQPVIEIAEFPHDNKLWRIDGLGKIFSQLKNNNNLFIEVYLR
                     PVNFHDNSVYNFAKFDSSGREIPEYNQLKKIPVNIGLLPILRVGSLWIKGKLQNNNAV
                     TDIATLKNIEINANTVEIINSNYKIKYLDSSGREQECKLLLQKNFATDAKVPFSCFKI
                     QNENYKNGIIIPVIEIIRFYYACSTRLAHLTFSNPDEITKISYAIDNETDKVVIKLPQ
                     QYTDQEAFILARILTSNIALSGLFQIKNSLIKIGVNEQPTTLLSTNFPFINPTNLMVR
                     GIKIVDRFLVTEICSCSAPFPDISVDRNNDNRKGDKKTDIPDDKKKEYRRGGQTPSLK
                     GNSTIQNQYESVNCVSTLQFNLNINCFSDLIGKKIEKPQKPFNEYKAQKLAINEKINV
                     TSLGVDLGTWSQTTIVPASISTFNTQSEKQNRDALAATFDNSVRAIEHLNEEYSDIQA
                     TIYMSSSRACEFVPLTDSAHKAQWSYLVSKTKTRRKVLLGKIEYKDKTFWLIEIQARK
                     SEHFVTAIIYFEAIDETKYLTQLLYVLAKQRGIWDKVPKNSSLQIITLKHTHKDIKSY
                     AQKIHKKISNFILK"
     gene            42212..43336
                     /locus_tag="ACFOA3_000126"
     CDS             42212..43336
                     /locus_tag="ACFOA3_000126"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFC6897208.1"
                     /translation="MKNRDTPHKKFINPTEYSLFAEEEQYDFELMIENQIKEHEKEID
                     YYVTDYPIETLVAKLDRQELFIPDYQRKNIWEKHRKSRFIESVLIGLPIPFLFFWQDP
                     STGNMEVVDGAQRLTALSEFIHGNLKLSYLEKLIYLNGQTYHQLLESRQKIFKNKPIR
                     GVVLSNKTDKEARLDLFERLNTGSKNATPAEIRRGLLQGAFYDMVETLSEDKTFNELA
                     KISKNMQDMGEREEFITRFFAYGEYLDHYSANVRKFIFDYTEIMNEEFAKNPNMRATY
                     EQKFHEIMAFFKEKYPIGFKRDGKGQSSRTRFEGMAVSAYLAEKENPGILAQVTPKEC
                     VDIFDSKEFIEVTGSDGANATKKLHARLFFAKNAFKRNHP"
     gene            43333..44085
                     /locus_tag="ACFOA3_000127"
     CDS             43333..44085
                     /locus_tag="ACFOA3_000127"
                     /inference="COORDINATES: protein motif:HMM:NF037890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFC6897209.1"
                     /translation="MNSVKSMMESREEEINLYMDLLKYIHDSTQHGLPALQNRNGVEY
                     QLENTHLHILTASIYMLLYNYIEAVVTQSLNTLICYIQNERMPITQFSEPIQKEWLIG
                     GLNLNDAKASVETRLGNGLKMLKHFGDTQKIDIKISKGGGNFNDDEIFKLAKKLQVNM
                     CIDDETHRQLRQAGNITYESTRKFQDTCMKEITRERNKLAHGEITFQECGRNRTISDL
                     MAIKDTTFRYMHLFTDAFTEYLMQKKYLKPTV"
     gene            complement(44069..45091)
                     /locus_tag="ACFOA3_000128"
     CDS             complement(44069..45091)
                     /locus_tag="ACFOA3_000128"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6897210.1"
                     /translation="MKIEAVDLFCGAGGLTAGLLRAGIKVKAGYDIDAACAYAYEYNN
                     KGAKFILQDVETVTGDQIQAHYSKDAVRLLAGCAPCQPFSTYNQGRDVRNDKKWPLLY
                     AFSRLIHEVQPELVTMENVPDVVKHEVYQDFVNNLKQQGYQVWAEKVLCADYGVPQSR
                     RRHVLLASRIGNIQMVPPTHSNCHVTVRQAIGHLPPLAAGKQDTIDVLHRCSSMSARN
                     LERIRASRPGGTWMDWPEDLRAACHRKKKGQSYKRIYGRMEWDKPAPTMTTLCIGFGN
                     GRFGHPEQDRAISLREAALFQSFPLDYQFTQETDTPIRQISRMIGNAVPVLLGEAIGN
                     SFKLLV"
     gene            complement(45152..46177)
                     /locus_tag="ACFOA3_000129"
     CDS             complement(45152..46177)
                     /locus_tag="ACFOA3_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HFC6897211.1"
                     /translation="MILPAHPKPYPDELFTSWLVRLAHANGLKVQTFCHLLFPNYEIW
                     NRDIDRHTPDWLVNTLAEKTGCSTIRIKTTTLDSLQGRLFDKNNFSGQLTWINSLKAN
                     HRKRDNNAIVYCPLCLATDHETYFRKSWRIALYTFCPIHQIMMHDCCPKCGIYIAFHR
                     QDLGKPNLQRFTELKYCWNCQYDLSNSPCENVIFFNDEIRLQWQNWLNQIANLDYLFN
                     QSEKEQLKILHHFLAITTSKKLAPDLYSYLCKQIHQIPVQLERSRRLSWESRSLAERH
                     STIHACLWLIQNFPKNLFKAWQDKAVRYNHLLKDFNDCPQSFQEIILKMNRNVHKELY
                     YSELIYY"
     gene            complement(46174..47067)
                     /locus_tag="ACFOA3_000130"
     CDS             complement(46174..47067)
                     /locus_tag="ACFOA3_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFC6897212.1"
                     /translation="MNYPHLSPKAKEQLKYSDIERIEYIRSPRWIGYPQAQQVLVKLE
                     DLLSFPKQNRMPNMLLVGDTNNGKTMLIKHFLRQHPAHDNPHGNGIIAPVVIIQAPPI
                     PDESRFYNAILDLLFAPYKTHDRTDKKLAQVLHLLKYVNTKMLIIDEIHHILSGSLNK
                     QKTFLNVIKYLGNELQIPIVGVGIKDAYRAIQTDAQLANRFEPAVLPRWELNKDFMRL
                     LMSFERMLPLSQPSDLHEISLATKLYGMSEGYIGELSRLLTQAAVAAIQQQTECINET
                     ILNSINWISPSQRKRQLDKLR"
     gene            complement(47064..48926)
                     /locus_tag="ACFOA3_000131"
     CDS             complement(47064..48926)
                     /locus_tag="ACFOA3_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6897213.1"
                     /translation="MKATLNVNVGAMVLFQNKICVITAVLELETYQLRDSVTGADYQA
                     KLADLSTVQSAELTHLDSIPEQKWQLAQQHFDVIEPLIRNPKRSKSDVQARAAECGVH
                     ISTVYRWLKSYKESGVLTALTRKQRSDSGKTQLSDDVEQLIRQVLEVDYLSTQRKSIQ
                     KIIREITRRCHKQNLPAPHANTIRSRIKNIAPQIKIAKRLSHKAAEMKFSPLQGSFPN
                     ADYPLSVVQIDHTKLDIILVDDIYRKPIGRPWITLAIDVFSRMVTGFYVSFDPPSALS
                     TGLCLAHSILSKESWLAKHGVKGRWPVWGLPRKIHLDNAKEFRGKVLEKACKQYGIEI
                     EWRPVARPHFGGHIERLLGTILDEVHGLTGTTFANTQQRKDYASESKAALTLTEFETW
                     LTLFICDVYHQRTHSGLGISPLAKWKSGVLGDDVSLGTGLPDKVVDETLLRLTFMPFE
                     MRTVQQYGVVIHKITYWHDVLRPWIAATNPTNPRQARQFIFRIDPRDLSVIWFYDPDL
                     SMYFPIPYRNSSNPVISIWELREIQRQLKNEQKQNIDENMIFTAYGRMRELEQQAQGK
                     TKAIRRAQQRRQLDQRTRASLPMPQIDSRNIISSENEENMFDDIQPFDDLDDLS"
     gene            complement(48923..49489)
                     /locus_tag="ACFOA3_000132"
     CDS             complement(48923..49489)
                     /locus_tag="ACFOA3_000132"
                     /inference="COORDINATES: protein motif:HMM:NF020304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFC6897214.1"
                     /translation="MSHLWSVIFLTLLEFDSNVQHFEVQPITIEWFDPAGKKHIYTPD
                     VLVHYQNDVIIYEVKYRSDLRKNWCELKPKFQAALHFCKLHGWHFKLITELEIHTDYL
                     CNVRFLLPYQQNGLSGEYSEAYMTLLDESLRELKKSTPKQLIQQTFQDEYNQAKLLPV
                     LWYLIAERKIGADLNQPLTMQSAIWFKK"
     gene            49783..49941
                     /locus_tag="ACFOA3_000133"
     CDS             49783..49941
                     /locus_tag="ACFOA3_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897215.1"
                     /translation="MEFTAKATAVAGIIKPGTPRQSEERFPAPAAPFDVNNINLNCPC
                     RLKALQTA"
     gene            50294..50758
                     /gene="bfr"
                     /locus_tag="ACFOA3_000134"
     CDS             50294..50758
                     /gene="bfr"
                     /locus_tag="ACFOA3_000134"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6897216.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMVRAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            50786..51259
                     /gene="bfr"
                     /locus_tag="ACFOA3_000135"
     CDS             50786..51259
                     /gene="bfr"
                     /locus_tag="ACFOA3_000135"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6897217.1"
                     /translation="MKGDRLVIRELNKNLGLLLITINQYFLHARILKNWGFKELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(51299..51598)
                     /locus_tag="ACFOA3_000136"
     CDS             complement(51299..51598)
                     /locus_tag="ACFOA3_000136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897218.1"
                     /translation="MLPIICFRMNVKVKHLLLRILLHIKAKKKGWRQPHPNTHTHQEE
                     RGKIKTDWETANKQSRFHIGIIKQKPLSSIDSNQFRFDSQTGKEKRHTRLYGYAV"
     gene            51585..51905
                     /locus_tag="ACFOA3_000137"
     CDS             51585..51905
                     /locus_tag="ACFOA3_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6897219.1"
                     /translation="MMGNKLTLPAELPAEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            51954..54512
                     /gene="glnD"
                     /locus_tag="ACFOA3_000138"
     CDS             51954..54512
                     /gene="glnD"
                     /locus_tag="ACFOA3_000138"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6897220.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKAKRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            54659..56122
                     /gene="guaB"
                     /locus_tag="ACFOA3_000139"
     CDS             54659..56122
                     /gene="guaB"
                     /locus_tag="ACFOA3_000139"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6897221.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREIILNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(56460..58835)
                     /gene="rnr"
                     /locus_tag="ACFOA3_000140"
     CDS             complement(56460..58835)
                     /gene="rnr"
                     /locus_tag="ACFOA3_000140"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6897222.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            58924..59010
                     /locus_tag="ACFOA3_000141"
     tRNA            58924..59010
                     /locus_tag="ACFOA3_000141"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58958..58960,aa:Leu,seq:cag)
     gene            59046..59132
                     /locus_tag="ACFOA3_000142"
     tRNA            59046..59132
                     /locus_tag="ACFOA3_000142"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59080..59082,aa:Leu,seq:cag)
     gene            59171..59261
                     /locus_tag="ACFOA3_000143"
     tRNA            59171..59261
                     /locus_tag="ACFOA3_000143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59205..59207,aa:Ser,seq:gga)
     gene            complement(59373..>59552)
                     /locus_tag="ACFOA3_000144"
     CDS             complement(59373..>59552)
                     /locus_tag="ACFOA3_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897223.1"
                     /translation="RFSYPIKKLPPFFQRHIAPSRFGIGFGFPAACRIMPFEGIRLLS
                     PYRLRKRIKRTIKKR"
BASE COUNT        15335 a        14950 c        14787 g        14480 t
ORIGIN      
        1 tgaatggttg gtatcggccc aatagtcgtc gctgtccaaa aagattaggt aatcgccttt
       61 tgccgcccgg ataccggcgt tgcgggcatc cgacagcccg ccgttttctt gatgaatcac
      121 ttttatatgc ggatatttgc ctgcatattc gtcgcaaatc ttcccgcagc cgtccggcga
      181 accgtcatcg accaaaatca tttcataatc ggtaaaattt tcggcaagca cggaatccac
      241 gcagcagcgg agatattttt ccacattgta aatagggacg attatggaga aaatcataaa
      301 tatcaatcca ttatattaag atgtttgcgc gtatgcctca aacccgcacc cgcaatgcgt
      361 ttgcatccac tataaaactt attgctttat caaggtatgg aaacctgttt cccgaaaggc
      421 ggcgcaggat gcccgttccc tgcatctttg ccttattccg acatcagatg ccccagcttg
      481 tctgctttgg tttggaggta gcgtttgttt tcaaggtttt ccccgacgtg cagggggatg
      541 cgttcgacca cgttaatccc cgcatctttc agggtttgga ttttttcggg gttgttggtc
      601 agcagtttga ccgagcggat gcccagatat tcgtagatag attgcgccaa acggaaatcg
      661 cgggcatcga cgggcagccc gagtgccaaa ttggcttcaa ccgtatccat accttggtct
      721 tgcagatgat aggcgcggat tttgttaatc agcccgatgc cgcgtccttc ctgacgcaga
      781 tagacgatga tgccgcgccc ctctgtctgt acggccctca tggccgcttc aagttgaggt
      841 ccgcagtcgc attttctcga gaacagcgca tcgcccgtca ggcattcgga gtggatgcgc
      901 gtcagaaccg gattgccgtc tgaaaaatca ccgacggtca gcgcgacgtg ttcctgccca
      961 tttgcctctt caaagccgtg catcgtaaat acgccccatt cggtcggcag gcggcaggat
     1021 gccacatggt tcaacatttc agacatcttc actcccatct tttccggcag acatacccaa
     1081 cagcagtttc aaaggttcgg acaaagccaa ggcaagagcc acccattcca cctgtgctgc
     1141 cgtacccgca cactctgcgc attcaaattc cacatgaacc acgcccaaca caccgccact
     1201 ttccgtgcag accggaatgg aaatttgcgc cgccgaagca tgattgcgtt ctcccgaaag
     1261 ctctcccaaa tccaaccaac gacgcacatc cgaggcaata ttcatccagc cgctttgcgc
     1321 cgaacggcaa gccagcgaaa ccttgcctgc cgcttcatcc aaatcccaca ggttttccaa
     1381 aacctcgccc tgccgggaca ggcaaaccag ccgaaaggcg gcattttcag acggcatcag
     1441 ggcataaacg gctgcggcgc gcacgccttt acagcgtgaa aacaccgaat ccaaagccat
     1501 gaaaagccgt ttcagacctt cctcattccc gctgttttgc tccaaataat cggcaagcct
     1561 ccaatcctca tgctcaagcc acaaaaccga acgctcaatc gaagccgtcc ccatatccat
     1621 taccgtctgc gccgtcagat acgccgcccg aacctcgtca agcggcagct tcaaaccctg
     1681 agtcagcaga aaatccttaa tcaaagcagc aggcataccc aaattccgtc aataaaaaca
     1741 aaaaaccgcc cgattccgat gtcaggcgga cgaaaaacag attttaccgc cccaaacccc
     1801 aagccatcaa cagccccgcc gaaaaccttg cacaaaaaaa caaaatctgt ataattgtcc
     1861 aattcccatg cggacgtggc gaaattggta gacgcaccag atttaggttc tggcgccgag
     1921 aggtgtgaga gttcgagtct ctccgtccgc accaataatg ataaataaat catatagtta
     1981 atgcttatcg ggacaaatta gtgccacaaa acataagtcg tctgcttttc agcgggcggc
     2041 ttttttatct gcctatatgt ttcaacacgc agaacggtac ataaagtgcc gccatttgtt
     2101 ccgcaccgaa tcggaaacgg gtgcttccgc ccgcaatcag attcactgcc cctaaatgcc
     2161 ggggtttcaa ccggaaagaa gaaaatattg attttgatga tggcatcaaa aaaccccttc
     2221 cgcccgtctg ccaaaaggaa tggttaagaa aagcaatgcg tcttttgatg gaacgcgata
     2281 tataaggaat tgaggggaag ggcaggcggg tttctgcccg ttaatcctgc ggacggtccc
     2341 gccaaacccg acacgcgcgg gcatatggcg gattgtttac aatattgtct aaaaatctga
     2401 acctgcaaac gtgaaataag gtagaatacg ccccgttatt ttaccgtcca atctccattt
     2461 accaaggaaa aacgatgagt acttcattga gttaccgcga tgcaggtgtc gatatcgacg
     2521 caggcgacca actggtcgaa aacatcaaac cgtttgccaa acgcacgatg cgcccggaag
     2581 tattggggga tttgggcggc ttcggcgcgt tggtcgaaat cggcaagaaa tataaaaatc
     2641 ccgtattggt cagcggtacg gacggcgtgg gtaccaagct caaacttgcc tttgattggg
     2701 ataaacacga tacggtgggc atcgaccttg ttgcaatgag tgtcaacgat attttggttc
     2761 aaggagcaga acctctgttt ttcttggatt atttcgcctg cggcgaattg gatgttccgc
     2821 gcgcaaccga tgtcatcaaa ggcattgcac aaggttgcga agaatccggc tgcgccctga
     2881 ttggcgggga aactgccgaa atgccgggta tgtatcccgt tggtgaatac gatttggcgg
     2941 gttttgccgt cggcgtggtg gaaaaagaga atgttattac cggccgcagc atcggcgtag
     3001 gcgatgtggt attgggtttg gcttccaacg gcgcacattc aaacggctat tcccttatcc
     3061 gtaaaatcat cgaacgcgac aatcccgatc tggatgccga gtttgataat ggcaaaacct
     3121 tgcgggaggc tgttattgcg ccgacccgtc tgtatgtgaa accgattctt gccgctttgg
     3181 aaaaatttac cattaaaggt atggcacaca ttaccggcgg cggcattacc gaaaacgtgc
     3241 cgcgcgtgtt gcctgaaaac acggttgcac aaatcgatgc taaatcgtgg gaattgccca
     3301 agctcttcca atggcttcaa aaggcgggca atgtggaaac ccaagaaatg taccgaacct
     3361 ttaactgcgg catcggcatg gtcgttattg ttgctgccga agatgccgat gcggttcagg
     3421 gtctcttggg tgaacaaggt gaaacagttt accgtttagg tttgatccgt gagcgtcagg
     3481 gagacgagca tcaaacccag gttgcctgat tgcttctata gcgaaatgcc gtctcaaaca
     3541 tgggtttgga cggcattttt atagaggatt aacaaaaatc aggacaaggc gacgaagccg
     3601 cagacagtac agatagtacg gcaaggcgag gcaacgccgt actggttttt gttaatccac
     3661 tatatattga ttgatatgag ggtgggttac acatgcttaa acgatataag ccattgtata
     3721 tttatgggat tcggaatgga acattttacc ctgcattagt atttccgctt tttattatgc
     3781 cgtctgaaaa tatcatgatg atgcgttttc agacggcata gctgatattg cagtatcaga
     3841 taggggcaaa cggaaaacac tgaaccgaac aatattactg ctatgccaag cgtacttatg
     3901 ggggcataaa cttacatgct tggtctgtta tccgaatgac cgtacgggga ttaaaacccc
     3961 taatatgccg tctgaacccg ccttattcca gcacgggttg ggggacgttg tgccggatgg
     4021 cgtaaacagc ggcctgcacc cggctgctga ggttgagttt gcggagcagg ttttgaacgt
     4081 ggactttgac ggtggattcg gcaagatcga ggtggcgggc gatgattttg ttgctgtgtc
     4141 ctgcggcgag atagcccaag atttccagtt cgcgaggggt aagtgaggag agtgcctgcg
     4201 tcccttgggc aggttggggg gaaatcaggc ttttgacgag tttggcggtc atctcgggcg
     4261 agaatacatt atcgccttcg gcggctttgc gtatgctttc gagcagaaag tcggcgttga
     4321 tgtttttcag caggtagccg cgcgcgccga tgcgcataca ttcggtcaag tcgtcgttgt
     4381 cttcggaaac ggtcagcata atcactgcct gctgcggatt gatgctgatg atttgggaga
     4441 gtgcttcgcg tccgttcata ccgggcatat caaggtcaag caggacgaca tcgggttgga
     4501 gccggttgat cattttaatg cccgagaggc cgtctgcggc ttcgccgatg acctcaaaat
     4561 catgttggcg cgacaaaagg gatttaatgc cgctgcggaa gagggtatgg tcgtctatca
     4621 ggataatttt aatggtcatt tcaagctttc ttcagatgca accgtcaatg agacggtggt
     4681 tccctgttga gcttgggaac ggatttctaa aacggcatgg atgcgtttgg cacgctcctg
     4741 catgatgtgc agtccgacat ggctgcccgt gggttctcct attttctccg tgtcgaaacc
     4801 ttgtccgttg tcttggatgg tcatggtaaa gcgtccgccg tgttcggaaa gggtgaattt
     4861 tacatgggtg gcgcgggcgt gtttgcggat gttggacagg ctttcctgca ggataaaaat
     4921 catttggagc tgcgcttcct gaggcggcag gaacgaaccg ttttcccagg cggtttcgac
     4981 cgttatcccg gtttgttgcg taaagcgggc gaataggtcg gcaacggctt cgggaaattc
     5041 tttattgctg attttggtac ggaagttgag cagcagttcg cggacatctt cataacattc
     5101 ctgcacgcct gttttgatga agccgatgtt ttctgcggct tcctcccgtt tgttttcggc
     5161 aaaggcggtt tccagcatct gtacctgtag gtttaggaac gttaatgctt gtgcgatgct
     5221 gtcatgtaat ccttgcgcaa tcaggttgcg ttcctgcaat actgcaagca ggcgtttttc
     5281 ttcttcctgc ttcgcgctag cgagcgatac gcccaattgc cgaatcaggg tttgaagcag
     5341 cgtgcggtcg tctccggtga gggaaatgcc gtctgaaaag ctgagcaaca gcctgcccaa
     5401 tgtttcgttc tggtactcaa tggggaagat ttcctcatgg tacttcccca aatccgaagc
     5461 tgctgtgccg caatccgcat gatgaatgga aacataaaca tcggattcgc cgtccaaaca
     5521 aactctgccg gaatctgctc ctacggcggg caggatacgg tttagaaaat gttctgcagc
     5581 ctgttgcggt atgtaggatt ggtgcaggtc ccgtgtagtt tggtacagca gggtcaggtt
     5641 ttgattttgt ttttcgagac tgcgtgtctg ctcggcgact tgtccttcca aatcatcata
     5701 taaaattttc aacctgccgc ccatttgatt gaaacaacgc ccgacctgtt tgaattccgg
     5761 cgtaccgcct tcgggaaccg gaatatcgaa acacctccgt ccgatgcgtt ccgcaccttc
     5821 ccttaacgcc tgcagcggcc ggataaccca aatctggtgc caaaacagca tcagtacaga
     5881 cgacaccagc gtcatcaaca taattgccca ttgaaaacgc ctgagccacc atgtgttttt
     5941 ttcgttggca ttttccaatg cctgcaaaaa cagttcgatg tttccggcaa agcggtagag
     6001 atcgacctga gtcggtcgcc ggtaggactg gagcgggggg aggatgtgtg cctgccaatc
     6061 tataatcagc atggattgta tcaaatcata agcaagaggg gtgtccgaag gaatcagcgg
     6121 atggatggca tcactttgtg aaatacgatt caggcttttt tcaaattcgt caacctgatt
     6181 gtctatttgg gagcggggag atccttcacc cgccatgtat gccagacggt atgcctgcat
     6241 tctcaagttg cccgcctctt cgatgacgga ggccgcgttt tccagacgca aagagagcag
     6301 cagtgtcaaa acgacagaca atgccgccaa cccgacccac actccggtca ggagtttcag
     6361 gcgtagggaa aggctgatgc cgtctgaaaa acgggctggc agtatcatgc tcggcgaaaa
     6421 attgttccaa ataatgcaaa caatatcatt ctttggaatt agatacaact gctcagaaag
     6481 aattggttaa ggaaaactta atccgcaccg cttcagtgtt aaatcatgtt atgataaaat
     6541 tcagataata tgaatttatt tgccgtctgc ttcagacggc attttgagtc attgggaacg
     6601 gcagactgca atcatgaaaa cctcgaactt ttccaagaga acatccgcaa tgaaaacttt
     6661 tgccctgata ctggccgacg gtcaggcgag ccgtatggga ggcgaggaca aggggcttgc
     6721 tcttttgggg ggtaaggcac tgatagatca tgtcatcgac agggtcaggc cgcaggtcag
     6781 ccatatcgcc atcagcacca accggaattt ggaagaatat gctcgaagaa gtccgcatat
     6841 ttttccggac gcacggcagt ggcagcattt cggcccgctt tcggcattgt gtaccgcagc
     6901 caacgatttg cagttggcga ctgccgactg gcttttggtt gtgccgtgcg atatgccgta
     6961 tctgccggac gatttggtgg cgaggtttga aaccgtgtcg aaacgcacac cgttgtgcaa
     7021 tgcgtattat gtggaaacgc cgataacgat gcactacaac attatgtata tccgcccgca
     7081 gattctgcaa agcgcgattc cctatctgtt ttcgggtatg aaaacattaa gaagctggtt
     7141 gcagcagcaa agggcgcgac cggtcagatt cgagtttgac gggcattttg ccgacttgaa
     7201 cacgcaaact gatttgcagg agggataagg gtaagaccgc cgaccggcgt ggaaaggaaa
     7261 ggtcaagccg taccgggcgg tttttgcccg aatcggaggc ataatgctgt ctgaaggcat
     7321 ttcagacggc atttttcgcg gggagatgct acaatttgca ccatttttac cgacacaggg
     7381 aaacaggatt atgtttacag gcattgttca aggattggga aaactgacgg caatccaccg
     7441 cccgtcggag gcatttcaga cttatgtcgt cgagcttccg caagaggcgg cggacaatct
     7501 gcaacgcggc gcatcggtcg ccaacaacgg ctgctgcctg acgattaccg aaatcgaagg
     7561 aaaccgcgtc agtttcgatt taatggcgga aactttggca aaaaccaatt tagggctgct
     7621 gaaggaaggc gattgcgtca acatcgaacg ggcggcgcgt ttcggagacg aaatcggcgg
     7681 acacgtcatg agcggacaca ttatggcaac cgtgcctatt gtcgaaatcg aacgggacgg
     7741 gtacaaccgc acggtttggt tttcgctccc gcacgaactc aaaccctata tcctgaccaa
     7801 agggttcgtc ggcttggacg gttgcagcct gaccataggc aaagtcgaag acagccgttt
     7861 caatgtccat ctgattcccg aaactttgga acggacgctg ttcggcagca gaaaggtcgg
     7921 cgacaggatc aacatcgaaa tcgatccgaa tacgcaggca atcgtcgata ccgtcgaacg
     7981 gctgatggcg caaagatatg caaagtgagg cagagatgga attgaacgaa tttctcgata
     8041 aagcctatgc cgttttgcgg cgtttggatg ccgtgcttcc gcccgaaccc gggcatacgg
     8101 attggaacgc gcttgccttc cgctggcaga gtgcgggcaa aaaaggtttt ttggaacact
     8161 tgcccgatcc gcacaccttt cccttggtaa ggctggcggg agtcggcagg caaaccgaat
     8221 tgctggtgcg taataccgaa cagttcattg tcggcagacc cgcgaacaat gtattgatga
     8281 gcggcgcgcg cggaacaggc aaatcctcgc tggtcaaagc cctgctacac gaatatgcgg
     8341 ataagggatt gcgcctgatc gaagtcgata aaagcgattt aatcggcctg ccttacctgt
     8401 tgacgctttt gaaggaacgt ccggaaaaat ttatcgtatt ttgcgacgat ttgtcgtttg
     8461 aaagcggcga tgaaacctat aaggcattga aaaccgcgtt agacggcggt ctgtctcaac
     8521 gttgcgccaa cgttttggtt tacgcgactt ccaacagacg gcacctgatg cccgaatatt
     8581 ttgacgagaa tgccggtacg acggggatgc ggggggaaat ccatcagaaa gaagcggtgg
     8641 aagaaaaaat atccctgtcc gaccgattcg gattgtggct cagtttttat ccgttcgatc
     8701 aaaacgatta tctggcggcg gtgcaaaatt ggctggaaga tttcggcgtg ccgtatgatg
     8761 aaaccgcaca gatggcggca ttgcagtggg cgcagacgcg gggcagccgt tcgggacgtt
     8821 ccgcgtggca gtttgcctgc gactgggcgg gcaggctgcc gaaacaacgg gcgttgtgag
     8881 cgtttcgggg attatttgag gcaggaagaa agccacattt gctgctgttg cggtgtgagg
     8941 atatggaaga accggtgttg gatttccaat tcgtccaccg caaaatccat accggacaaa
     9001 tagcggcttt cgacataatc gcgcgcctcg ttccgattaa aaacatccga ggaaatgatt
     9061 tcgacgacag accggcggcg gctgtgttcg gaatgcataa ccttcaaacg cgccctgtcg
     9121 cccgccattt tgaaggcggt gcggatttta cgcagctcat tgtgctgact ttgggtcagc
     9181 ccgagtcggc gtatgtcgca gttcggttga aaatcgttcg gggggatata agccgtggcg
     9241 gcgtgggaaa gctgacagga aagcaaagcc atacttaaaa aagaggcggc aggtttggca
     9301 aaacggcagg gagcaggcag tggcacggct tttcctttaa tccagatatt tcggacaata
     9361 tacttgattg ccgtgtgaaa gatggtatta tggcagttaa cttttgttaa taatgctgtt
     9421 tcggggcttt cccgcgttga gtccgcccgt aaaggcattg aaggaaaaaa tatgaccgcc
     9481 taccgtatcg cacccagtat tttgtcggca gactttgccc gcctcggaga agaggtggaa
     9541 agcgtcattg cggcaggtgc agatctgatc cattttgacg tgatggacaa ccattatgtg
     9601 ccgaacctga ccttcggccc tatggtttgc gcggcgttga agccttatgc aagcgtgccg
     9661 atcgatgtgc atttgatggt cgaacccgtt gacgacctga ttcagtcgtt tgccaaagca
     9721 ggagcatcga ttatcacttt tcatcccgaa gcgagccgcc atatcgaccg cagcttgagc
     9781 ctgattcgtg atatgggctg tcaggcgggg ctggtgttga atccggcaac gcccgtatat
     9841 ctgttggaaa acgtattgga caggctggat atggttttgc tgatgtcggt caaccccggg
     9901 ttcggcggtc agagcttcat tccgcagact ttggaaaaaa tcagacaggt tcgcgccatg
     9961 cttgactgct atgaagagaa aagcggcaga cgtattgcca ttgaagtgga cggcggcatc
    10021 aaaaccgaca atattgccgc cgccgcccaa gccggcgcgg atacctttgt ggcaggttct
    10081 gcgattttcg gcaaaccaga ctataaggcg gtgattgatg cgatgagggt tgaattggca
    10141 aaagcggcag gctgaacctt gcgccaatga ttgaaataga aatgccgtct gaaaattagc
    10201 tctgtttcag acggcattgc gttatttttc gacgggcagc ccgaaatgca ggtaggcgcg
    10261 ttcggtcgcc atcctgccgc gcggggtgcg ttgcaggaag ccttgttgga taaggtaggg
    10321 ttcgataacg tcttcgatgg tgtctgttga ttcgccgatg gcggcggcaa cattgtccaa
    10381 accgaccggg ccgccgccga atttgtgcaa aacggcttcg agaaatttcc tgtccatcac
    10441 gtccagcccc tgcgcgtcca cgtccagcat gcttaaagcg gcatcggcga tgccgccgtc
    10501 gattgtgccg ttgtttttca cgtcggcgaa atcgcgcacg cgtcgcaaca ggcggttggc
    10561 gatgcgcggc gtaccgcggc tgcgtttggc gatttcttcc gcgccttctt cggacatatc
    10621 gagctgcaac agttgtgccg aacggctgac gatggtggta aggtctcggt tttcgtaaaa
    10681 ctcaaggcgg gagacgatgc cgaagcggtc gcgcaacgga ttggtcagca taccggcgcg
    10741 ggtggtcgcg ccgacgaggg tgaagggcgg caggtcgatt ttgacggaac gggcggcagg
    10801 cccttcgcct atcataatgt cgagccggta gtcttcgagc gcgggataga ggatttcttc
    10861 gacaacaggg ctgaggcggt ggatttcgtc gatgaacaat acatcgtgcg gatcaaggtt
    10921 ggtcaaaagg gcggcgaggt cgcctgcgcg ttcgaggacg gggccgctgg tttggcgcag
    10981 gttgacgccc agctctttgg cgatgatgtg cgccagtgtg gttttgccca gtccaggcgg
    11041 gccgaagagc agggtgtggt ctagggcttc gccgcgtttt ttggcggctt ggatgaagat
    11101 ggcaagctgt tctttggctt tgtcttgccc gatatagtcg tccagcgttt taggacgcag
    11161 ggcgcgttcg agcagttctt cctgtgcgga ggcggtttgg gcggcaacga tgcgttgcgg
    11221 ttgcgcggcg gtcaggttgt cggtttgcag catagtgttc cctttgtcgg gtatgccgtc
    11281 tgaacggtcg gcggcgtttc agacggcatt gaaaagataa cggtcaaaag cgttttaccc
    11341 gcgcgtttcg cgccaaacga gatagtcgcg caggggcggc ataggcgggt tgatgcagtg
    11401 tgggcagatg cctgtggtgt cttcgtcgtc gctgtcgatg tgataggcgt tggggtcgaa
    11461 gcggacttgc tgcatcaccg cctgcgccaa tgcctgcgcc tgaacaacgt cgaaattgaa
    11521 tttttcgagg atttcgttca acaggcggat ttcgccaccg ctaaattccg cactgtgttt
    11581 gaggatgagg cgttgataat cttcgttgtt gattttaggc aataaatcgg ttttcagggt
    11641 catgatttta tgtcgggggc aggatggctg aagcgtgaat tatagcggat atggcggcgg
    11701 ctgttgcaaa gcttggattg ggtatcctcc tcaaaacttt atagtggatt aacaaaaacc
    11761 agtacagcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
    11821 cctgattttt gttaatccac tataaaagtg gctgcattgg gcgttttaat gtttttctgt
    11881 tttgtcttgc gccgctcgaa acgctttttg cagcggggcg atttgcgcca gccgcgcccg
    11941 gtcgatttgt tcggcgataa ggtgcgcttt tccctgcgcc cagcattcgg cggcgatttt
    12001 gcccgaatct acctgattgg cggcttcgag cagggcgagc cagtgcgcgc gttgcgggta
    12061 gggcgtgtgt tcgcggttca gacggccttg cgtgtcggca atgcagacgt tcaatgccgt
    12121 ctgaaagcgt tcgggtcgtc tgaaagcgtc ggtttttttc aatacgttca gaatggtttg
    12181 gcttttaagc tgtccgactt ggtggaaaat aatgtgccaa cggcaaacca attcggcaag
    12241 ctcggcacaa tgtttcggcg cacgcagcct ttgattgact ttgcgcacgg gttcgacacc
    12301 ggcgaggtcg tgtccgtggt ggcgcggcag gatgtcggac ggtgttttgg ctttgcccaa
    12361 gtcgtgcagc agggcggcat agcgttcggg caggctcaaa cccatatcgg cggcgcgttg
    12421 cagcgtcatc agggtatgga tgccgctgtc gatttcggga tggtagtcgg cgcgttgcgg
    12481 cacgccgaag agggcattga cttcgggcag caagactttg agcgcgccgc attcgtgcaa
    12541 cacttcaatc attttgcgcg gatttttttc catcaaacct ttcgcaaact cctgccagac
    12601 gcgttcggca accaatgcgt ccgcttcgcc gttttccacc atctgccgca tcagctttat
    12661 ggtttcttcg gcgatttcaa acttgtaacg cgcggcaaag cgggcagtac gcaggatgcg
    12721 gacggggtct tcggcaaagg ctggggaaac gtggcgcaaa atgcctgccg ccaaatcccg
    12781 ttgtccgccg aaagggtcga taatcttgcc gtccgcatct tgcgccatcg cgttgatggt
    12841 caggtcgcgg cgcatcaaat cctgctccag cgtaacgtct ttgtcggcgt ggaaactgaa
    12901 accgacgtaa cctttggcgg ttttgcgctc ggtgcgggcg agggcgtatt cttcgtgtgt
    12961 ttcgggatgg agaaacacgg gaaagtcttt gccgactggc tggaagcctt gcgccagcat
    13021 ggtttgtgcg tctgcgccga cgaccaccca atcgcggtct ttgacgggca agcccaaaag
    13081 ataatcgcgg acggcaccgc cgacgagata agtctgcata tgcttcctat tttaaagtta
    13141 tcaacaatgc cgtctgaagc cgcttcagac ggcattgttc caaccggcgg ttatgctacg
    13201 ccttttttct cgagatagct ttcgtaatcg cccaagtagt gttcatatcc gcctttgccg
    13261 tccaattcaa tgatttgggt agccagggaa gaaacgaact gacggtcgtg ggagacgaaa
    13321 atcagcgtgc cgttgtattt ttccagcgcc atgttcaagg attcgatgct ttccatgtcc
    13381 atgtggttgg tcggttcatc catgactaag acattgggtt tcaacagcaa cagtttgccg
    13441 taaagcatac ggcctttttc accaccggag agaaccttca cttttttcac gacatcgtta
    13501 ctgccgaaga gcaaacgccc caaagtgccg cggatgactt gttcgtcgtc gccgtcctgc
    13561 ccccattggc gcatccattc gctcaaatcc atatcgacat cgaagtcgtt ttcgtgatct
    13621 tgcggatagt agccgacgct ggctttttcc gcccatttga tgctgccttc gtccggcagc
    13681 agaccgtctg aatattcggg gttgtacgcg ccggccaaga gtttcagcag ggtggatttg
    13741 cccgcgccgt tcgggccgat gatggcgagg cgttgtcccg cttcaaggat gaagttcagg
    13801 tttttaaaca actgggtttc aaagcgtttc gccagttttt caacttccac agcctgacgg
    13861 tgcagcttgg ctttttcatc ggcttcaaaa cggatatacg ggttttgacg ggtagagggt
    13921 ttgacctcga ccatctccga tttgattttg tctgcctgtt tcagacggct ggttgcctga
    13981 cgggctttgg atttgttggc actgaaccgc gccacaaatt cttgcaattc ctgcaatttt
    14041 tccttcgcct tggcattgtc tttcagggcg cgttcgcgcg attgggcgga ggcgagcata
    14101 tagtcgtcgt agttgcccgg atagatggtg atggtgttgt aatccaaatc tgccatatgc
    14161 gtgcagactt cgttcaagaa gtggcggtcg tgcgagatga taatcatcgt ggagtcgtat
    14221 tggttcaaca cgccttccaa ccagcggatg gtattgatgt ccaagttgtt ggtcggttcg
    14281 tccaagagca atacatccgg tttggagaac agggcttgcg ccagcaatac gcgcagtttg
    14341 aagcccgggg cgacttctgc catggtcgca ttgtgcaaat cttcggaaat gcctacgccg
    14401 ctcaacaact cggcggcgcg cgcttcggcg gtgtagccgt cgtattcggc gaacttggct
    14461 tccagttcgg cggctttcat gtagtcgtct tcggtggctt cgggattggc gtaaatcgca
    14521 tcacgctcgg tcatcgccgc ccacatttcg gtatgcccca tcatcaccac gtccagcacg
    14581 cgcatgtctt cgtaggcaaa ctggtcttgg cgcaatttac ccaaacgcac gccgttttca
    14641 atcgccactt cgccggtcgt ctgttccaaa tcgccgccga ggattttcat gaaggtggat
    14701 ttgcctgagc cgttcgcgcc gatcaaaccg tagcggttgc cttcgccgaa tttaacggat
    14761 acattttcaa acagcggctt cgcgccgaac tgcatggtga tgccgttggt agaaatcatg
    14821 attggtaaag cctttattga acaagagatt aaatttttcg aattttcgga ttgtaacata
    14881 accgccccgc cgtctgaaac agacgggcaa attttcagac acttatagta aaatatccgt
    14941 tttattattt cagacaaaag gtttccaaat gataatcctt cacggcattc caaattgcga
    15001 cacagtcaaa aaagccaaaa acaggcttgc cggatacggc ttggagtttg aatttcggga
    15061 ttttaaaaaa cagaggccgt ccgaagcgga aatctgctcg tggctggaac aagtgccttt
    15121 ggcaaccctg ctcaacaaac gcgggacaag ctggcgcaaa ctcgatgccg aaacacagca
    15181 aaaagccctg ttctcgacag tggaggccgt caaactgatg tccgaaatgc cgagcctgat
    15241 caagcgtccc gtattggagt gcggcggcaa ggtttacgcc ggcttcagcg aagaaaccta
    15301 cgacggcatc ttcaaccggc aagccccgtg cagacaggga taaaaaccgt tacaataccc
    15361 gacttgaatt tcccgttccc attctatatc ccgatttaaa atatgttcca ttccatcgaa
    15421 aaatacagaa cgcccgccca agtcttatta ggcctgattg cattgacctt cgtcggcttc
    15481 ggggtcagca cggtatccca tccgggtgtc gactacatcg tccaagtggg cgacgaaaaa
    15541 atcagcgagc agtcgcttaa cgccgccgtc caaaacgaac aggcgggcgg cggcggccct
    15601 tcgcgcgacg cggtgttcca atccctgctg caacgcgcct acctgaaaca gggcgcgaag
    15661 ctgatgggca tttcggtttc ttccgaacaa atcaagcaaa ttatcgtgga cgatcccaat
    15721 ttccacgacg caaacggcaa attcgaccac gcgcttttaa accgctacct ttcccaacgc
    15781 catatgtctg aagaccagtt tgtcgaagaa atccgcgatc agtttgcctt gcagaatttg
    15841 gtaaacctcg tccaaaacgg cgtattggtc ggcgacgcgc aggcggaaca gctgatcagg
    15901 ctgacgcagg tcaaccgcac catccgttcg cacactttca accccgacga attcatcgcc
    15961 caagtcaaag tgtctgaagc cgatttgcag aagttttata acgcaaacaa aaaagactat
    16021 ctgctgccgc aggcggtcaa attagaatat gtcgccttga atctgaagga ttttgcagac
    16081 aaacagaccg tcagtgaaac ggaagtgaaa aatgcgtttg aagagcgcgt ggcgcgtttg
    16141 ccgacaaatg aagccaaacc ttctttcgag caggaaaaag ccgccgtcga aaacgaattg
    16201 aaaatgaaaa aggcggttgc cgacttcaat aaggcaaaag aaaagctggg cgatgacgcg
    16261 ttcaaccatc cttcctcgct tgccgaagcc gccaaaaaca gcggtttgaa agtcgaaacc
    16321 caagaaactt ggctgagcag gcaggatgcg caaatgtctg gtatgcccga aaacctgatc
    16381 aatgccgtat tcagcgacga cgtattgaag aaaaaacaca attccgaagt gctgaccatc
    16441 aacagcgaaa ccgcgtgggt cgtccgcgcc aaagaagtcc gcgaagagaa aaccctgccg
    16501 tttgccgaag ccaaagacgc ggtacgtcag gcttatatcc gtaccgaagc cgccaaactt
    16561 gccgaaaaca aggcaaaaga cgtgcttacc caactgaacg gcggcaaggc tgttgacgtg
    16621 aaatggtcgg aagtgtccgt tttgggcgcg cagcaggcaa ggcagtccat gccgcccgag
    16681 gcttatgcgg aactgctgaa agcaaaaccg gcaaacggca aacccgccta cgtcaggctg
    16741 atcggtctgc cggcacccgt gattgtcgaa gtacaggctg taaccccgcc ggatgatatc
    16801 gccgcacagc ttccgcttgc aaaacaggct ttggcgcaac agcagtctgc caatactttc
    16861 gacttgttga tacgttattt caacggcaaa atcaaacaga ccaaaggagc gcaatcggtc
    16921 gacaacggcg acggtcagta attgacactt ttgttgacaa aataacggtc ggaatattcc
    16981 ggccgttttc ccatacggcg gaatatgatg agccacaaaa aacaagatgc cttccaagga
    17041 ttgatcgacg cgctgaaggt tttacccaac gtcgggccga aatcggcaca gcggatagcg
    17101 tatcatttgc tccaacacaa gcgcaaagag gctgaaaaac tggtggatgc cttgcagacg
    17161 gcattgaagc aggtttacca ttgcgcgatg tgcaacacgt tttgcgaagg cggattgtgc
    17221 gatatttgtg ccgatgaaac acgcgacggg cggcggctga tggtggtgca tatgcctgcc
    17281 gacgtgtcga atatggaagc ggcaaactgc cacgacgggc tgtatttcgt cctgatgggg
    17341 caaatcaata cggcattggg aatggacgta tccgccatcg cattggacag gctggcgcaa
    17401 cggctgggcg gcggggaagt cgaagaaatc attattgcaa ccgcttttac cgcagaaggc
    17461 aatgcgacgg cgtatgtcct gtccgagttt tttaaaaacc tgccttacaa agtcagcagg
    17521 ctgtcgcagg gcattccctt gggcggcgaa ttggaatatg tcgatgcggg aacgctggcg
    17581 caggcggtgt acgaacgccg cctgattaaa gaaggcgggg cataacgccg ccaatgaaaa
    17641 atgccgtctg aagccttcag acggcatttt tccgggcggt ttacttgcag tttagccctt
    17701 gaatctgcaa cgcgcctccg ttgccgactt tgaacacgca ctgcgtcgcg ctgctgccgg
    17761 acacggtaat gctgccgctg ccgccgttca ggctgatagg ttccttaaca cccataccga
    17821 ctgccgttgc cgccagcttt tgcgcgtcgg cttcggaata aggcgtgttg ttggcatcga
    17881 ttttgatttc tttggcgtag gcagccgcag cggcggataa ggcgatggcg cacaaagcaa
    17941 aagcagttcg gatattcatt gttttcctca agaaacggtt tttctgaagg aaaggatagg
    18001 gcagtttgac gcgttgtgca agttttccgc ccgctctgcc gccccgtccc ccgcactcgg
    18061 gcgttcggac gctgccgggg ttttacggta caataaactt ttgccgccgc cccccaaacc
    18121 gccgtccggg gcggcacgtt tgcagacatt tttaaggtag cgttatgttt tctctagagg
    18181 cttggatagg cttgaggtat ctcagggcga aaaagcgcaa cggctttatg tcgtttatca
    18241 cgatggtttc gattgccgga atcgccttgg gcgtaaccgc gctgattgtc gtcttgtcgg
    18301 ttatgaacgg ctttcagaaa gaaatacgcg ggcagctcct gaatgtcgcg ccgcacgccg
    18361 aaatcggcta tatcgacaat acggatacgg attggcgcaa cctgcttcgg tttaccgaaa
    18421 accgcaaagg tattttggct gccgcgccct atgtttccaa tcaggcattg ctggccaatg
    18481 cgggcgaaat caggggcgtg cagatgcgcg gcattttgcc gtctgaagag cgcaaagtgg
    18541 tggaatacgg cgacaaaatg ccggcaggca aatttgaaga cctgattccg ggcgagtttg
    18601 acattatcct cggtgtcggc ttggcggagg ctttgggggc ggaagtcggc aataaagtta
    18661 ccgtcatcac gccggagggc aacgttaccc ctgccggagt cgtgccgcgc ctgaagcagt
    18721 ttaccgtggt cggtctggtt aagacgggcg tttacgaagt ggacaactca ttggcgatga
    18781 cgcatatcca agacgcgcgc gtactgtacc gcttggataa ggaagttgcg gggctgcggc
    18841 tgaaactcgc cgacccgcaa aacgcccccg ccttgacggc aacactgatt ccggaggcgc
    18901 aaagggacgc ggtttgggtg cgcgattgga cgtacaacaa ccgcagctat tttgaggcgg
    18961 tcgagttgga aaaacggatg atgttcatca tcctgacgct gattatcgcc gtggcggcgt
    19021 tcaaccttgt ctcttccttg gtgatggcgg ttacggaaaa gcaggcggac attgcgattt
    19081 tacggacttt gggtctttcc cctggcggcg tgatgaagat ttttatggtg cagggcgcgt
    19141 tttcaggctt tttcggcacg ctggcgggtg tggtctgcgg cgtgcttttg ggttggaacg
    19201 tcggcagggt cgtggcgttt tttgaaaacc tgctcggtgt ccacctcatc aattcgcagg
    19261 tttattttat cgactacctg cccagcgatg tcgatatggg cgacgttgcc ctgattgcct
    19321 gcatttcttt gggactgtct ttcgtggcga cgctctaccc gagctggcgc gcgtcaaaaa
    19381 cccaaccggc ggaggctttg cgttatgagt gagttgattt tgaaatgcga aggcgtgggc
    19441 aaacgctacc gtgacggcgg tttggacgtt cgggtgctgc acggcttgga tttggaaatc
    19501 ctcgcggggg aaagtaccgg catcatcggt tcttcgggca gcggcaaatc gacgctgctg
    19561 catattttgg gcgggctgga tatgccgtct gaaggcaggg tgctgctgat gggcgaggat
    19621 ttgcgtacat taagccagcg gcaactgggc accctgcgca accgccatct cggtttcgtg
    19681 taccagttcc accatcttct gcctgaattt tcggcactgg aaaatgtgat gatgccgctt
    19741 ctgatcggca aaaaaagccg tgaagaggca gcagaaacgg caatggcgat gctggaaaag
    19801 gtcggactga aacaccgttc gaggcaccgc gcgggcgaac tgtccggcgg cgaacgccag
    19861 cgtgccgcca ttgcgcgcgc cttggttacg caaccaaaat gcctgcttgc cgacgaaccg
    19921 accggcaacc tcgaccgtgc gaacgccagg aatgttttgg atatgatgct ggaactgaaa
    19981 acagaattgg gaacggggct ggtggtcgta acgcacgacg acgaactcgc cggctgcttc
    20041 gggcgcgtga tggtcatgaa agacggcagc ctgcacccca aacagggcgc aaatgcctaa
    20101 acagccgaaa caacacgcgc cgtctgaagc ctcgcgcctt cagacggcat ttcgacaagg
    20161 aatacaatgg aacggcagac agatgaattt gcccaagcgg cggcaagggc ggcaatccgt
    20221 tttttggaac actatgcggg ttccggcgac gaagtgcttg caaactgcac cgaacgcctg
    20281 tttcaggcat tgcaaaacgg tcattcgttt atccgtttga gcggtggcga ggctgacgcg
    20341 ctgtcggcac tcgcacccgt ggtcggaaca tccgccgcgc ctttgatttt ggaaggcaga
    20401 aggctgtttt tgggcaggat gtggcagttg gaatacgatt tggctgccga gataaaacgc
    20461 ttggcggcag ccggcacgtc cgcccccgac gcggcaggcg cgcggcaaaa cctcgcaaaa
    20521 tggtttcaag gcacgggcag cgaagggcag cgcgatgccg ccgccttggc actgttgcag
    20581 tcttttatgg tgattaccgg cgggccagga acgggcaaaa cgacaacggt tgccaaactg
    20641 ctggcgctga tttgcggtga agacgaaaat cttcccaaca tcgcgcttgc cgcaccgaca
    20701 ggcaaagcgg cggcacatat ggcgcgcgcg ctgcaccgtg caatcaacgg ttttgacgcg
    20761 ccggaggccg tccgccgcca tttgctcaaa ctggaagggc aaaccgtcca tcgattgctg
    20821 aagctgcgcc cgcccaaaat gcaggcggcg ttcgaccata tccgtccgct gccgttcgat
    20881 gtgctgattg tcgatgaagc ctccatgttg gataccgcat tgatgctgca acttttgaaa
    20941 gcggtcaaaa ccggcgcgcg cgtgattctg ctgggggatg aaaaccagct cccgtccgtc
    21001 ggaatcgggg cggtgctgtc cgttttgtcg caaaaaacca ttttggacag agaaacgtat
    21061 aaaagtttga acctctacct gcccgaacac ggatttccgg tacacgaaga tgctcccgtg
    21121 ctggcgcaaa acaccgccca tctgtcgttc agccaccgct tcggcgacaa cagcggcatc
    21181 ggctgccttg cccgtgccgc cgtatcgggc gatgaggggg cgtgggcatt gtttgaccgg
    21241 tttccggacg aactggaaca ttcggaatgc agtccgaacg ctcaagtcga aaggttgtac
    21301 cgggcacaca aagcctattg gcaggcggta aaagacggca atatcgaagc cgcatacgtg
    21361 ggcatctcgg atatcgtggt tctggcagct tggcggcagg attcggaaga cttcaacgaa
    21421 gcctattgcc gccatgtacg ccgcaaaatg aacataccgg aacatcaggc ctatttcgcc
    21481 ggagaaccga ttatgatcag acagaacgac tacgcgcttg aactgttcaa cggcgacatc
    21541 ggactgatta tggaagatgt cggacggcag ggcagccttg ccgcctattt tgccgatgcg
    21601 gacggattta aaaaggtagc ggtaagctgc ctgcccgaat ttgaacccgc attcgccatg
    21661 accgtccaca aaagccaagg ttcggaatat cgggaagtct ggctgctgcc gccttccgcc
    21721 gcaccttcgg acgaagggga cgatgcattg tccggattga gtaaggagct gttatatacc
    21781 gccattaccc gcgcgaggga gaagttcgta ttcttcggcg gggaagaagc cttccggcaa
    21841 gctgccgcca ccgtcaaaac gcgtcagacg gcattgggca gtatgcttga gcgggtattt
    21901 tcacaagaat aatccgcccg aatgatgtcg tgccggcggc gcgggcgttc cgatgcctgc
    21961 cgccggaagc gcaccgccgc cccttatgcc tttttcaaac ggtacaggaa agtagtttcc
    22021 ctgtttcggg caaaagccag cggattgttc ggatcggcag cttttttgtg aatcggtttg
    22081 gttttcaaaa catccgcaac gcgcaaaccc gcctgagcaa atgccttatc cagaaaatcg
    22141 gacggacgca ggtgcagatg ttcagcaagg tcggaaatga tcaggcggat ttcgccgtcg
    22201 ggattcagat gtttcggcgc atcccttaaa aacgcggcca acatcgcaga atcggggtca
    22261 tataatgcgg attcgacggc ggaagtcggc ttggcgggca gccacggcgg gttgcagaca
    22321 atcaaatcgg cacgcatatc ggggaacata tcggtttccc gtatttcaac ctgtttttcc
    22381 aaacccagcc gggcgatatt ggcccgggcg caggcaaccg ccctcggatt ggtgtccgtc
    22441 cctattacgg aaggaatccc ctgttttgcc aatatcgcgg caagcacgcc cgaacccgtc
    22501 ccgatgtcca atgccgtctg aaaccctgtt gacggcgctt gggcgagcaa atcgagatat
    22561 tcgccgcgca agggcgaaaa tacgccgaaa ggaacgtgta tgctgccgcc cagctgcgga
    22621 acggcaaccc ctttcttatg ccactcgtgc gcacccataa accccagcag cagattgagc
    22681 ggtaggaaaa acggtttgcc gtccgcctct ccgtacacgt cgagcaaagc ggagcgtata
    22741 tcgggcgcgc gtttgttgtc caacacaaaa ccggggcgga tttcaacggc aagcatattc
    22801 agaatacggc tctgctgtgc ctgcttcata cggtgggcat ggaaaagggc ggcgatatcc
    22861 gcatcggaac ggacggcggc aggtttgcga accctcttct tcattgcaga aagcacctgt
    22921 ttggcattgt ggaaatcgcc ctgccagaca gttgcaatat tttgataggc agccttcaaa
    22981 atgccgtctg caccgctttc gcggacataa tgccaacctt tgggcggctt ttgcagactt
    23041 tcgttgcgcc attcgaaccc gttatcggga aaaataaaag aagacatggg atacctgcgt
    23101 catgttttga aaatagggcg gcagaaccgt aaaccatacg gatggtacag caaggagcgg
    23161 caacacagaa cagttttttg ttcctgcctt gtctttccaa gcccatgccg tctgaagcca
    23221 gaatgtttca gacggcatcg catcaaactc cataaataaa ccacatatgc ttgaaataat
    23281 accttcaacc ccaatgtacg cgaaaatcgg caatctgtca gacacaagag agtacctatg
    23341 acacaaaaag aaaagcattt tgaggaatat gccgccttgg caacccttcc tttgcgggat
    23401 gtcgtcgttt acccgcatat ggttctgccg ctgtttgtcg gcagaccgaa atccatcgcc
    23461 gcactggaaa acgccattac ccgcgaggag ccggttttcc tgttggcgca aaccgatgcg
    23521 gcggtagaag aaccggttgc cgccgacctg tatcagaccg gtacggtcgc acaagtcctg
    23581 caagtgttga aactgcccga cggcacggta aaagtattgg tcgaagggct gtatcgcgga
    23641 cgtgttctga ccatcgaaga cacgggcggg ctgttcgttt cccatataga ggcggtcgtg
    23701 gaagaagaca cgggcggcaa taccgacctc gaagccgtgc gccgcaccct gttggcgcag
    23761 tttgaacaat acgccaaact caataaaaaa atccccgccg aaatcatcgg cagcatcaac
    23821 ggcattgccg aaaacagccg gctaaccgat acggtcgcag cgcatttgca gttgaaactg
    23881 gcgcaacgcc aacagatttt ggaaattccc gaaatcggca aacggatgga attcctgctg
    23941 gcacagctgg aatccgaact cgacattatg caggccgaaa aacgcatacg cggacgcgtc
    24001 aaacgccaaa tggaaaaatc ccagcgcgaa tattatctga acgaacagat taaagcgata
    24061 cacaaagaac tgggcgaaga agacgaaaac ggcgaactgg atgccttgga agcagatatc
    24121 aaaaaggcgg gtatgaccaa agaagcggaa gaaaaatgcc tgtccgaact gaaaaagctc
    24181 aaaatgatgc cgccgatgtc tgcggaatcc accgtcgtac gcaactacat cgacactttg
    24241 ctcgagctgc cgtggaagaa aaaatcccgc gtcagcaaag acatcgccaa agccggactg
    24301 gtgctggatg ccgaccacta cggcctggaa aaagtcaaag aacggatttt ggaatacctc
    24361 gccgtccaaa aacgtatgga caaactcaaa ggcccgattc tgtgcctggt cggccctccg
    24421 ggcgtaggta aaacctcttt gggcgaatcc atcgccaaag caacggggcg gaaatatgtc
    24481 cgcatggctt tgggcggcgt gcgcgacgaa agcgaaatcc ggggacaccg ccgcacctat
    24541 atcggctcta tgcctggtaa gattctgcag aatatggcaa aagccggcgt aaaaaaccca
    24601 ttgttcctgc tcgacgaaat cgacaaattg ggcaacgact tccggggcga tcccgccagc
    24661 gcgttgctcg aagtgctcga tcccgaacaa aacaacaagt ttgccgatca ttatgccgaa
    24721 gtggattacg atttgagcga tgtgatgttt attgcgactt caaacagcct gaatatcccg
    24781 accccgctgc tcgaccgtat ggaaatcatc cgtctgtccg gctataccga agacgaaaaa
    24841 atcaatatcg cgatgcagta cctcgtgccg aagcaaatga agcgcaacgg cgtgaaagaa
    24901 ggggaattgg caatcgaaga aagcgcggtg cgcgatatta tccgttacta cacccgcgag
    24961 gcgggcgtgc gttcgctcga ccgcgagatt gccaaaatct gccgcaaggt ggtgatgcag
    25021 attaccttgg acgaagataa gaagaggttg tctgaaacca agaaaaccag caaagccaaa
    25081 cctaaagcgg ttaaagtaaa tgagaaaaat ctgcacgact atttgggtgt gcgccgcttc
    25141 gattacggcg ttgccgaaag cgaaaaccgt atcggacaag taaccggtct ggcgtggaca
    25201 gaagtcggcg gcgaactgct gaccgtcgaa gccgcagcat tgccgggtaa gggcgtgatt
    25261 cagtgcaccg gccagttggg cgatgtgatg aaggaatccg tgtccgcagc atggtcggtt
    25321 gtccgctccc gcgcggaatc agtgggcttg gctcctgatt tttacgagaa aaaagacatc
    25381 catgtccatg ttcccgaagg tgcaacgccg aaggacggtc cgagtgcggg cattgccatg
    25441 actttggcga tggtgtctgc attttccaaa atcccggtac gcgccgatgt agcgatgacg
    25501 ggcgaaatta ccctgcgcgg cgaagtcctg ccgattggcg gtctgaagga aaaactgttg
    25561 gctgcgcttc gcggcggcat caaacacgtc ctgattccga aagacaacgt caaagacttg
    25621 gaagaaatcc ctgaaaacgt gaaaaccggc ctgaccatcc atccggtcaa atggatagac
    25681 gaggtattgg ctctgggttt ggaaagccag cctgagcctt gggcagaacc ttctggtgtg
    25741 gaagcggcgg cggaatccgc ttcaaaacca aaaccccgca gcagggcaac caaacattga
    25801 aacgcaggaa atgtgttgta aaaatgcggt ttcgtcctga aagcctgtca aatagggtga
    25861 ttccgtattt tttgcttgac acggcaattt cagaattgct ataaagcgaa agttgctcaa
    25921 gcagtacaaa ccccgagtat tcgggcattt catcattttt taagacagga agggactgat
    25981 tgtgaacaag tctgaattga tcgaagcgat tgctcaagaa gccgacattt ccaaagccgc
    26041 cgcacaaaaa gctttggatg ccactaccaa tgcagtaacc accgccctga aacaaggcga
    26101 caccgttact ttggtcggtt tcggtacttt ctacgtgggc gaacgtgcgg aacgccaagg
    26161 ccgcaacccc aaaaccggcg agcctctgac cattgccgcc gccaaaacgc ctaaattccg
    26221 tgcaggtaaa gcattgaaag acgcactgta agccgttttt tatgaaaaaa gccgattctt
    26281 taaagaatcg gcttttttat cggtccacat tattctgatt tcaaatcggc aacacactgc
    26341 ttgtcacgtg cttcaaaggc atttgcgccg ccgagcaggt caagctgttc ttgtgcgccg
    26401 agtttgccga aggatctaat ctgtttctcg ctcaatctgt ccaaaggctg ctcccacata
    26461 catttgcagt agtcgacggc gaggcgggta ttgtttgaat ctaaaccgcg ggcccgcaaa
    26521 tcgttttgcc atttttcggc aaacggaata ttcttcacgc aagactcgac aattttctgt
    26581 tttgcctgcg gtttggacat cgcgcattgg gagagcaggg cggttaaagc gagcaacgcc
    26641 aaaatgaccc atgcccaaat gcggatggtg cggattttgg cttttgcttt tttgcgcgcg
    26701 gcaacctgct cttccgtcgg catttcgtgt ttcggctcag tcatgcaggc tttccatgcg
    26761 gatcatggta atcggttttt ccacgcaatc cagtgcttcg atggctgcga ttgccgactt
    26821 gatgtgtttt tcgaccgtgc tgtgggtcag aatcacgatt tcggcagtgg tctgatcaat
    26881 cacgcctttt tgaatcagtg cttcgatgga gacgttttct tgtgccaaca gcgcggcgat
    26941 ttgccccagc gtgcccggtt cgtctttggc ttggacgcgc aggtagtagc tgctggtaat
    27001 ttcgtccata ggcaggatgg tttgcgcttg gacttgcgcg ggttggaacg ccagatgcgg
    27061 tacgcggtgg gcggtatcgg cttcaaccag gcgggcgatg tcgatgatat cggcaaccac
    27121 ggcggaagcg gtcggcaatg cgcccgcgcc cgcgccgtaa tataaggttt cgccaaccat
    27181 atcggcgttg acgcgcacgg cgttcatcac gccgttgacg tttgccaaga ggcggctttc
    27241 gggaatcagg gtagggtgga cgcgcagctc gatgcctttg ccggttttgc gggtaatgcc
    27301 caacagtttg atgcgatagc caagttcttc ggcgtatttg atgtcgcggc tgtcgagttt
    27361 gctgatgcct tcgaggtagc aggcggaaaa gttcatcggc gtgccgaatg ccagtgcgct
    27421 catgatggtg attttatggc ccgcatcgtt gccttcgatg tcgaaggtcg gatcggcttc
    27481 ggcataaccc aatgcctgcg cttctttcag tacatcggca aacgcgctgc ctttttcgcg
    27541 catttcggag aggatgaagt tgctggtgcc gttaataatg ccggcgatgg atttaatcct
    27601 gtttgccgcc aaaccttcgc gcagggcttt gatgattggg ataccgcccg ctactgccgc
    27661 ttcaaattgg acgatgacgt tttgtttttc cgccagcggg aagatttcgt tgccgtattc
    27721 ggcgagcagt tttttgttgg cggtaacgat gtgtttgccg ttttcaatgg ctttcaacac
    27781 cgcatctttg gcaatgccgg taccgccgaa caattcgacg acgacatcga cgtcttcacg
    27841 tgcgaccagt tcgaacggat ctttgacaaa ggctgcggac gggcaggttt gtcgggcttt
    27901 ttcttcactc aaatcgcaca cggcagaaat acggatttcg cgccccaagc ggcgggaaat
    27961 ttcctccgcg ttgtcccgca acacggcagc cgtaccgccg ccgaccgtac ccaaacctaa
    28021 aagaccgatg tttactggct tcattgtgtc tccttgtaag ccgactgaaa tgtaaatatt
    28081 gaaagacgaa aatatccgct gccgatataa ttgtgccgca ctttgaatca aatgccgtct
    28141 gaaatcggca ggcgggtcag atgaaatctg ccaatcctac atgaatttgt ctgattttgc
    28201 acccctttcg gtgtagatga tgcggcgacg gggtaaaaaa aatgttgttt gaagaaaatc
    28261 cgatagacgg acagtttgcg gaatatgaat gcggtgcggg cggaatccgg ctggcgaggc
    28321 aaagtttcca taaacccgtg cttgtacata aggattcggt ctgcctgtcg caatgccgaa
    28381 ccttgtccga tctgactccg gaaaacctgt tgtccgacat caaacctgtt gactatccgg
    28441 aaatattgat tatcgggacg ggcgcggctc aggagtttat ccatcccaaa atcatggcgg
    28501 atttttcccg aatcggaatc ggcgtggaat gtatgaatac cgattcggca ttcaggacat
    28561 tggttttcct gcactcggaa gggcgcaggg cttgggcttg gcttcagccg taaatttccc
    28621 gtttcagacg gcatcggcac tgactttcag gtaaaatacg ggcttttccc gcccgacgat
    28681 gtttccgtta tgattgaaat caaaaacctc accctgcaac gcggtttgaa agtcctgctc
    28741 gacaaagcca acgctaccgt caatcccggt cagcgcgtcg gtttgatcgg caaaaacgga
    28801 acgggcaaat cgagcctgtt tgccttaatc aagggtaaaa tcactcagga cggcggcgat
    28861 gtctcgattc cgaaaaactg gcggctcgct tccgtttccc aagaaacgcc cgatttggat
    28921 atttccgctt tggattacgt tttgcagggc gatgccgagt tgcaggcttt tcagacggca
    28981 ttgaggcagg cggaagcgca aaatgacggc atgaagcagg cggaatatca tgccaaattg
    29041 gaagaaatcg acgcttatac cgcgccggcc cgcgcggcaa aattgttgaa cgggctgggt
    29101 ttttcgcaag aagaacacag ccgccccgtc aaatcctttt ccggcggctg gcgtatgcgc
    29161 ctcaatctcg cccaagccct gatttgccgc gccgatttgc tgcttttgga cgaaccgacc
    29221 aaccacttgg atttggaaac cgtcttgtgg ctggaaaacc atcttgcttc tttaccctgc
    29281 acgcaaatca tcatttccca cgaccgcgat tttctcaacg cggcaactac ccaaaccatt
    29341 gaattatcgc agcaaaaact cacgcaatac ggcggcaatt acgattttta ccaaaacgaa
    29401 cgcgcgcagc gtctcgcgca acaacaagct gcctatgtca aacagcaggc gcaaatcaaa
    29461 catctgcaat cctttatcga ccgcttcaaa gccaaagcca ccaaagccgt tcaagcgcaa
    29521 agccgcatga aggctttggc aaagctagaa cgcgttgcgc ccgcgcatct ggacagcgag
    29581 ttttcctttg agttttacca tcccgaccat ctgcccaatc ctttgttaaa gctagaacac
    29641 gctgatttgg gttacgaagg caaaaccgtt ctgcatgaca ttaccctgtc gctggaaagc
    29701 ggcgcacgct acgggctatt gggtgtcaac ggcagcggta aatctacctt tatcaaagcc
    29761 ttggcgggca aaatcgattt gctttccggc agcatcgtcc gttccgaaaa actcaatatc
    29821 ggctattttg cccaacacca actcgatacc atccgcgccg accaaaaccc tgtttggcat
    29881 atccagcagc tttctcccga agtacgcgaa caagaaatcc gaaacttcct cggcggcttc
    29941 aactttgccg gcgatatggc gttgcagaaa accgaacctt tttccggcgg agaaaaagcc
    30001 cgtctcgccc ttgccatgat tatctggcaa aagccgaacc tgctgctgct tgacgaacca
    30061 accaaccatc tggacttgga tatgcgccac gccctgacgc tcgctctgca aagtttccaa
    30121 ggcgccttaa tcatcgtatc gcacgaccgc agcctgcttg aagcgactac cgacagcttc
    30181 ctcctgatcg acaaaggccg tctgaagaac ttcgacggcg atttgaacga ttaccgccaa
    30241 tggcgtttgg cacaggaaaa cgccgccgcc gcacccgcag catccgcaca aagccaaagc
    30301 cgcaaagaca ccaagcgcat cgaagcgcaa atccgtcagg aaaaagcccg acgcggcaag
    30361 ccgatacagc agaaaataga ccgtgccgaa aaagaaatgg cgcagctttc cgaaattcag
    30421 acggcatgtg aagcattttt agcacaagaa gacgcttacc ttgaagcaaa caaagaaaaa
    30481 ttgcaaaata ccttatccca gctggcaaaa gtcaaaacac aacttgccca aatcgaagag
    30541 gtttggctgg cttgtcaaga agaattggaa cggattgaaa ctgaaatcga gaaacagttt
    30601 gccgagcgat aaagaagcgc gggcagggtg cttatctttg cctgccaata acggtataat
    30661 tcgggcgtta tcaccgcctt ttaccggtat aaacatcaga ctttttgccg ccttgggtct
    30721 cctgtccctt tccggcgcgg cggcgcaagc ctccgtatac cattgcaact caaatggcaa
    30781 aagcgtatat acatcagccc cagcaggatc gtgcgccgat gcggatttac ctaaaatcag
    30841 cagccatcag ggaggcggat accgcctgaa aattaaaaaa ctcagttaag aagccaaaat
    30901 acacatagaa agtaaaaaga aaaacaaaaa acctgccggg aaaaagaaca agcaggctgc
    30961 caaagccccg aaagaaaatc aaaagcaacc aaccgaaaag aaaagcccgt aaaacgccaa
    31021 gaaagcatta caaaaaatac caaaaaatca aataattatc cgagcatcaa gcacattatg
    31081 aaatcaaaac tccccttaat cctaatcaac ttttccctga tttcaagccc attgggtgcg
    31141 aatgcggcca aaatctacac ctgcacaatc aacggagaaa ccgtttacac caccaagccg
    31201 tccaaaagct gcctctcaac cgatttgccc ccaatcggca actacagcag cgaacgctat
    31261 atcccgcccc aaacatccga accgacacca tcaccgtcaa acggcggaca ggctgtcaaa
    31321 tataaagccc cggtcaaaac agtatccaag ccggcaaaat ccaatacgcc cccgcctcaa
    31381 caagcacctg taaacaacag cagacgctcc attctcgaag cagaattgag caacgaacgc
    31441 aaagccctga ctgaagccca aaaaatgtta tcacaagcac gtctggcaaa gggcggcaac
    31501 atcaaccatc aagaaataaa tgcattacaa agcaatgtat tggacaggca gcaaaatatc
    31561 caagcactgc aaagagaatt gggacgtatg taaagccgtg ttttcaaatc gaccgttcca
    31621 aggatttgac agaagaaatg atgaaaaagc agcggcatat cctgtgtgtc ggctgcctgt
    31681 atattatatt gaaggtcaac gtatttccca ataccggctg gatggaagca tcgaaaggga
    31741 tgcagggcat gtcgttcagt accttcggat acgcggatgc tccgggctat acattcagcc
    31801 gcattggcca tactttaatt ttcctacttg gaaagaccac agggacacgt acaactgttt
    31861 ccgatagttc gcataatgta tattatgtta aattatatat ttgtgcaaat aactccaagg
    31921 cattaagctg ttgtttgatg ctttgccagt ttgggagaaa gttgtccata tgcgccataa
    31981 aacgggcgtt gcgatggcgt tcgagcaaat gggtcagttc gtggacgacg acgtattaag
    32041 cggtgcggac acccgaaccg cgatccccta aatgtcttgg tgggaattta ggggattttg
    32101 gggaattttg caaaggtctc aaaacccgct cgccttatag cctgtctgtg ccgccatttc
    32161 agcctccaaa acccatattt tcaaggtggg cattgacttt gctttgccac ttttccactt
    32221 ctcgggcaag ctcggccatc gggctgcggt agcggtcttc cagcgtgttc aagcggctga
    32281 taagctgctg aatactgttt tccaagcgat tttcgattcg gctttgtaaa tcggcaagcc
    32341 atttgtcttg aacggcaagc tgcttgattt cggcttcgga aagtcggccg aattgtttga
    32401 atacggcaag gttcagggct tcgatttggg ttttgactgc gtctttcgcg gctttttcct
    32461 gcgtcatcag tgtttgggtg gtttgtaaaa cagcccgttc gccttcttct atgccgcttt
    32521 cttccaatgc ggttttcaga agtttggcgg aaagtttgcc ttttgcatcc aatacgtcgt
    32581 tcagcgcacc ctcttcgccg ccgtgttctt ctatgtggtt ttccaattct tggcttaggc
    32641 gttccagctc gctttgtttt tcttccagct tggcgatgcc gtctgaaaag tagcggcggg
    32701 caaccagctc gggggcgatg acttcgctgc ggtatttttt gctgatgcgc ttggttttgg
    32761 ctttgccttt tttgccggtt tcggtttcct caaagacgac ggtcaggttc gcggcttcat
    32821 cgctttcttt ggtgatttcg gccaggtttt taaccgcctt ccagccatct tgggcgatga
    32881 gataaacatc gtcttgcagg gtttccgccc agtagtccgt caggatttgg tagaaatcgt
    32941 attcttcaat caggctgccg ggtttgaacg cgtccagcag gctttcgctc catttccgga
    33001 taagcctgcc cggccggctg cccggttgga tgacggcaag gtcgttttga gtgtgccacg
    33061 cggcaaactt tgccaggtgt ccggctttga aggcggcgta atcggggtgc gccaatatat
    33121 gggctttgat ttggctgctt tcgattttat agtggattaa atttggggct gtactagatt
    33181 agccctaaat tccacaccaa tcccgcagga ttttaagctg ttgagacggt gtgccgaagt
    33241 taaatcgaaa ttcgcattct ttcaagaaca gtgggaaaga tttgcaatcg attccgttgt
    33301 atagtggtaa agcggccatc gtgttcggca aacaactcgt ttttcatacg gcctaaaact
    33361 tgccaatagg ctttcaatgc gtccatatcg tgcgcaggta tgccgccatc cataaagata
    33421 ggcggcgaga ttttacaggt cttcgacttc tccgctgtcg atatagcgag gcagattaag
    33481 gttgtaatct tgtgctgcga tttcgcttaa atgcaccata cggctgtaac ggggaatttc
    33541 ttcctgtttt gtgaaggtgt cgatgatttt gtgaatgtct tgttcgcgca gacggttttt
    33601 gttgccgtct ttaataaagc cgcgcgatgc gtcaatcata aacacgctgc cgccgctgat
    33661 aacttggttt gttccctctt cggcaaattg ggcggtttgg gcgtgttctt tgtcgatgac
    33721 gatgatgcag gcaggaatgc ccgtgccgta aaacaggttg gcaggcagcc cgataatgcc
    33781 tttaataagg tcaaggttaa gcaattccgt gcgaatgcgc gcttcggcat tgccgcgaaa
    33841 cagcacaccg tgcggaagaa taatcgcacc tttgccgctt ggtttcaggc ttttgagcag
    33901 atgcagcaaa aaggcgtaat cgccgttttt ttcgggcggg ataaggcgga ttggcaacgg
    33961 caaaatcgaa ggtcttaagc ccgtcgtttt catcacggaa agacgaatcg gacaaggtgt
    34021 tccggtgttg atttcggcgg tttcgttgtt gtgcaaaatc attttcatac gggcaaggga
    34081 cgcggttgcc acatcttttt cctgaccgta aaggctgatt tggctgccgg cttgggcggc
    34141 ggctttgagc aacagcgaac ccgagccgca ggtcgggtca taaacgctgg tgctggacgg
    34201 gcaatctgcg ctgattccga taatcttcga gacttcggca ggcgtgtaaa actgcccttt
    34261 ggatttgcct gactcggtcg caaaatggcg catcaggtat tcgtaggcat cacctaacaa
    34321 atcgtcgtct tcggcttggt tggaagaaag gttgagcttt tcaaaaatgc caacaagcct
    34381 gctcaaacgg tcgatcatct ccttagcttt acccagtttg tcttcgtcgt tgaagtcggc
    34441 aacgtcgatc acgcctttta agtcgttggc ttcggcaatt tgggcgatga tcttattcag
    34501 gcgatcgccg atgtcggcgg tgtttttgag tttgacgatg tcgtcaaaag tcgtaccggc
    34561 gggcagctca atcatgccgc cgccttgctt gtgtttgtcg gaaacgtatt ttaaaaacaa
    34621 tagcgtaaga acatagtctt tataaaggct cgcatccata ccgccgcgca actcgtcgca
    34681 gcccgcccaa agtgatgcat aaagctgatt tttttttgat tgccataagg ttttgcctaa
    34741 taaatggaaa ttacaaatca aacgcgattt taacacgccg gcggcaacgg ctatatcggc
    34801 attcacagct tccagtttat cggatcgata ccgtgccggc tcaaatagct gtttgcctgt
    34861 gaaaaatggc ggcagccgaa aaaaccgcga tatgccgaca gaggagacgg atgcggtgcg
    34921 gtcaatatca aatggttttg actgtctatc agtttccttt tttgttgtgc atacccaccc
    34981 cacagcataa agacaagatg cttgcggtgt gtcgcaagct gcctgatgac ggtatcggta
    35041 aagcgttccc agcctaaaag ggcgtgcgaa tgcgcctgtc ctgcacgcac cgtcaaaacc
    35101 gtgttcagaa gtaatacgcc ctgctccgcc cacgctgtca ggcagccgtg cgcgggtatg
    35161 gaaaagcctt cgatgtcggt ttccaattct ttgtagatat tgagtaaaga cggcggtatg
    35221 cggataccct gccggacgga aaatgccaaa ccgtgcgcct gccctgcccc gtgatacgga
    35281 tcttgtccga gaatgacggc tttgacccgg tcgaacgctg tcaggcggaa tgcgttgaac
    35341 acatccgccg ccggcggata gatgatttgt cccgacaaac gttcctgcct gactgcattt
    35401 aaaatttcct gaaaatacgg ctgctgtttt tcgccgccga gtgcatcgtg ccaagtgtcc
    35461 atatctgccc tatcctttca aattcggcta tactgctgat attgcgttat tttgtcaaac
    35521 gacgcgcttt gtggggaaat gtatgactgt atggtttgtt gccgctgttg ccgtcttaat
    35581 catcgaatta ttgacgggaa cggtttatct tttggttgtc agcgcggctt tggcgggttc
    35641 gggcattgct tacgggctga ccggcagcac gcctgccgcc gtcttgaccg ccgctctgct
    35701 ttccgcgctg ggtatttggt tcgtacacgc caaaaccgcc gttagaaaag ttgaaacgga
    35761 ttcatatcag gatttggatg ccggacaata tgtcgaaatc ctccgacaca caggcggcaa
    35821 ccgttacgaa gttttttatc gcggtacgca ctggcaggcg caaaatacgg ggcaagaaga
    35881 gcttgaacca ggaacgcgcg ccctaatcgt ccgcaaggaa ggcaaccttc ttatcatcgc
    35941 aaaaccttaa cactcggagg aattatggaa tttttcatta tcttgttggc agccgttgcc
    36001 gttttcggtt tcaaatcctt tgttgtcatc ccacaacagg aagtccacgt tgtcgaaagg
    36061 ctcgggcgtt tccatcgcgc cctgacggcc ggtttgaata ttttgattcc ctttatcgac
    36121 cgcgttgcct accgccattc gctgaaagaa atccctttag acgtacccag ccaggtctgc
    36181 atcacgcgcg acaatacgca gctgactgtt gacggcatca tctatttcca agtaaccgac
    36241 cccaaactcg cctcatacgg ttcgagcaac tacatcatgg caattaccca gcttgcccaa
    36301 acgacgctgc gttccgttat cgggcgtatg gagttggaca aaacgtttga agaacgcgac
    36361 gaaatcaaca gtactgttgt tgcggctttg gacgaggcgg ccggggcttg gggtgtgaag
    36421 gttttgcgtt atgagattaa agacttggtt ccgccgcaag aaatccttcg ctcaatgcag
    36481 gcgcaaatta ctgccgaacg cgaaaaacgc gcccgtatcg ccgaatccga aggtcgtaaa
    36541 atcgaacaaa tcaaccttgc cagtggtcag cgcgaagccg aaatccaaca atccgaaggc
    36601 gaggctcagg ctgcggtcaa tgcgtcaaat gccgagaaaa tcgcccgcat caaccgcgcc
    36661 aaaggtgaag cggaatcctt gcgccttgtt gccgaagcca atgccgaagc catccgtcaa
    36721 attgccgccg cccttcaaac ccaaggcggc gcggatgcgg tcaatctgaa gattgcggaa
    36781 caatacgtcg ccgcgttcaa caatcttgcc aaagaaagca atacgctgat tatgcccgcc
    36841 aatgttgccg acatcggcag cctgatttct gccggtatga aaattatcga cagcagcaaa
    36901 accgccaaat aagccgtgat gaaaatgccg tctgaagctc tgttttcggg gttcagacgg
    36961 catttttatt taaacccggg caaggttgta ttccgcttca aatatgtttc ggaaccgcct
    37021 acctgccccg gtaaaaactg ccggcaaaat atttctgcac gccctcgcga agcaagacca
    37081 aggttacggc ggccaaaggc aatccggcca acattccgac aaagcccatc agctgcccga
    37141 acgccatcag cgaaaagata acccaaaacg gcgacagccc gatacggtct cccacgattt
    37201 tcggcgtaat gaaaaaactt tcgagaaact gtcctacggc aaaaaccgcc caaaccgata
    37261 ggatgccgtt ccacgaaccg aactggagca aggcggcgac ggttgccagc agcagtcctg
    37321 taaacgcgcc caaataggga acaaaaacca aaatgccggc aagcataccg atggcaaacc
    37381 ccgaatccag cccgaccagc accaacccca agccgtaaac caaacccata atcagcatca
    37441 ccagaagctg cccgcgcaaa aattcgccca atacctcgtt caaattgcct gtaatgcgcg
    37501 tataagcacc ggcaaaacgc ctcggaacca gtttggcaat gccgcacgac caccgctgcc
    37561 aatccagcag gaaatagtaa agcagcaagg gaagcagcag caggttgccg atactgctga
    37621 caatattgcc gccctgcctc atcaaaacgg gaaaccacgc cttaagcgcg ttgctcaact
    37681 ctcccgtatg cgcctgaagc cacgcaataa tagatgcctg atcgatttcc acatatccgc
    37741 cgattgtatt tttcaaccac ggcagcagcg tgttctgcat aaaaccgatt aattggggca
    37801 ggcgcgatgc caaattgttg aactgcccga ccagcatagg gacgataatc aacaataatg
    37861 ccaacaacaa aatcaaggaa aacaccatca cagacatcga agcggatgca cggttcaaac
    37921 cctttttctg caaccattcg accaaagggt ccaatacata cgccagcacc gccgcaaccg
    37981 caaacggagt caaagtatcg ccgagcgcga aaaccagcca aaccaaggcg gcaaacgccg
    38041 caccggcacc catccacggc ttgatgcccc gccctttcct ccgatacata aaaaccaccc
    38101 ttctaaaaat aatattgcgc gccagtatag cagaaccgcc gcgccgtcaa aaaagcctcc
    38161 gccttcccga tagcctgtta tcgcggttta ggctaaaata acgcacatcc gatgccgtct
    38221 gaaaggcaaa cccgccttca gacggcatac cgtacacgat aaggcagcac atcatgaccg
    38281 aacaagaaaa caaaacatcc ctcatcgaat ttccctgcac cttcccattg aaagtaatgg
    38341 gcgcggtgca tcctgagttc gagcaggcag tattggacac cgtccgtctc cacgcccctg
    38401 acacgcaggc tcaccacatt accacgcgtc cgagcagcaa aggcaactat accggcgcca
    38461 ccgtacaggt aaaggttgaa aaccaagaac aattggacaa catctaccgc gcgctgactt
    38521 cgcacgaact ggtcaaagtg gtactttgag atgaaaatca tacataaagg tttggttgaa
    38581 tatctgccga cttttgaggc aatgaaaacc tttaatgccg gacgtaatgc cgacaccgaa
    38641 gacgaactct gggttgtcga gcatcccccc gtgttcacac aaggactggt gggaaaaccc
    38701 gaacacctgc tgattcgcga cgacattccc gtcgtccaaa tcgaccgggg cgggcagatt
    38761 acctatcacg ggcccgggca attggtcgtt tatacgatga ttgatttcaa acggcgcaaa
    38821 accagcgtcc gcaacatcgt ttccgcgctt gaaaacagca tcatcgccac attggcagaa
    38881 tacggcatcg aagcggcggc agaccccaaa cgccccggcg tttatgtcgg agaacgcaaa
    38941 atcgcctcac tggggctgcg tatcaaaaac ggctccgtct atcacgggct tgcgctcaac
    39001 gtcaatatgg atttaagccc gtttacccac atcaacccct gcggctacgc cggtatggaa
    39061 atgacgcaaa tcgcggattt tgtccaaccc tgccccacgc cggacgaagt cgccgccaaa
    39121 cttaccgcac accttgaaac acaattcaca ccgaaagcag acaacaatga gtgaaatcaa
    39181 aaccgacgac cccaaacgcg gcatcaaact cagaggcgcg gacaaaaccg cccgcatccc
    39241 catcaaagtc gtcccccttc aggaaaaatt aaaaaagccc gaatggatac gcgccaaact
    39301 tccatcgcgc aaattctttg aaatcaaaga cattttgcgc gaacaaaaaa tgcacaccgt
    39361 ttgcgaggaa gcctcctgcc ccaacatcgg cgaatgcttc agcaaaggca cggcgacctt
    39421 catgattatg ggcgacatct gcacccgccg ctgcccgttc tgcgacgtgg gacacggccg
    39481 cccgaatatg ctcgaccccg acgaaccgaa aaacctcgcc gaatccgtca aagccatgaa
    39541 cctgcgttac gtcgtcatca cctccgtcga ccgcgacgac ctgcgcgacg gcggcgcaca
    39601 gcatttcgcc gactgcatca aagccatccg cgaaaccagc ccgaacacca aaatcgaaat
    39661 cctcgtcccc gacttccgag gacgcttgga catcgcactc aaaatccttg ccgaaacccc
    39721 gcccgacgtg atgaaccaca acctagaaac ccatccgagc ctgtacagaa aagcccgtcc
    39781 cggtgccaat tatcaacatt ctttagactt attaaaacgt tataaagaaa tgatgccgca
    39841 catcccgacc aaatccggca tcatggtcgg cttgggcgaa acagacgaag acgtgcgtga
    39901 aattatgcgc gatatgcggg cgcacaatat cgaaatgatt accatcggac agtacctcca
    39961 gccttcagac ggacacctgc ccgtcctgcg ctacgttacg cccgagcagt tcaaaatctt
    40021 tgaaaaagaa gcatacgaac tgggcttcag caatgccgcc atcggcgcga tggtacgttc
    40081 cagctaccac gccgacgaac aggctgccga agccttacgc gaatgtgaat tctgacgcta
    40141 tttgagactg aactacgcta tttgcgactt tataacgcta agttcgactg ggcaaatatg
    40201 gatcaacaac cagttattga aattgccgaa ttcccacatg ataataaatt gtggcgaatt
    40261 gatgggttag ggaagatttt ttcccaattg aaaaataaca ataacctgtt tattgaagtc
    40321 tatttacgcc cagttaactt tcacgataat tctgtatata actttgctaa atttgatagt
    40381 tctggtagag aaattccaga atataatcaa ttgaaaaaga ttcctgttaa tataggcttg
    40441 cttcctattt taagagtggg atctctttgg ataaaaggaa aacttcaaaa caataatgca
    40501 gttacagata tagccactct taaaaatatt gaaataaatg caaatacagt agaaataatt
    40561 aatagcaatt acaaaataaa atatttagat tcaagcggaa gagaacaaga gtgcaaacta
    40621 ttgttgcaga aaaattttgc aacggatgca aaagtacctt tttcttgctt caaaattcaa
    40681 aatgaaaatt acaaaaatgg aataattatt ccagtaatag aaattatccg tttttactat
    40741 gcctgttcaa ctagactagc tcatttaaca ttttctaatc cagacgaaat tacaaaaatc
    40801 tcatatgcga tagataatga aacagacaag gtagtcatca aattaccaca gcaatataca
    40861 gatcaagaag cctttatttt agcaagaata ttgacttcaa atattgcttt atctggactc
    40921 tttcagatta aaaattccct aataaaaata ggagttaatg aacaaccaac tacattatta
    40981 tctaccaatt tcccatttat caatccaacc aatttaatgg ttcgcggtat taaaattgtt
    41041 gatagattct tagttacgga gatttgttct tgttcggctc cttttcccga tatttctgtt
    41101 gataggaata atgacaatcg aaagggagat aaaaaaacag atatacctga tgataaaaag
    41161 aaagaatatc gaagaggggg acaaactcct agtctcaagg gaaattccac tattcaaaat
    41221 caatatgaat cagtcaattg cgtatcaaca ttacaattta acttaaacat aaattgcttt
    41281 tcagatttga tcggtaaaaa gattgaaaaa ccccaaaagc cctttaatga atacaaagca
    41341 cagaaacttg ccataaatga aaaaattaat gtaactagct taggagtgga tttaggtaca
    41401 tggagccaaa ctacaattgt gcctgcgtcc attagcactt ttaataccca atctgaaaaa
    41461 caaaataggg atgccttagc tgcgactttt gacaattccg tacgtgctat tgaacatttg
    41521 aatgaagaat attcagatat tcaggcaact atctacatgt ctagctctcg agcttgtgag
    41581 tttgttccat taactgactc agcccataaa gcccaatggt cgtatcttgt ttccaaaacc
    41641 aaaacaaggc gtaaggtatt attaggaaaa attgagtaca aagataaaac attttggttg
    41701 attgaaattc aagctcggaa aagtgagcat tttgttacgg cgattattta ttttgaggca
    41761 attgacgaga caaagtattt gactcaatta ttatatgtct tagcaaaaca gcggggaatt
    41821 tgggataaag ttccaaaaaa ctccagtttg caaattatta ctctaaagca tacgcataaa
    41881 gatatcaaga gctacgctca aaaaatacac aaaaaaatat caaattttat cttaaaataa
    41941 attagttagt aatataacta atctagttat aaattaccaa aatacttcag gctgatactt
    42001 ttatataaaa tttaagttac ctactctctt attgataagg ctggtggtgt cgtaactact
    42061 atataggagt aatggttccc atctgctaac ataaatgtta aggtaagagt aaattctaaa
    42121 gacacaaaag aaaacggttc ttttgatatt tcaaggttat gtttttaatc tataacataa
    42181 tattaacaat ttctattttt aggtattcaa aatgaaaaat cgagatactc cgcataaaaa
    42241 atttatcaac cctacggaat atagtctatt tgcagaagaa gaacaatatg attttgaatt
    42301 aatgattgaa aatcagataa aagaacatga aaaagaaatt gattactatg taactgatta
    42361 tcccatagaa acactagttg ctaaattaga tcgacaagaa ttgtttattc cagactacca
    42421 gagaaaaaac atttgggaaa aacatcgtaa aagtaggttc attgagtctg ttttaattgg
    42481 cttacctatc ccatttttat ttttttggca ggatccttca actggaaata tggaagtggt
    42541 agatggtgca caacgactaa cagcattgtc tgaatttatt catggcaatt taaaattatc
    42601 ctatttggaa aagcttattt atcttaatgg ccaaacttat caccaactcc tagaaagtcg
    42661 tcaaaagatt ttcaaaaata aacctatccg aggcgtagta cttagtaata aaacagacaa
    42721 agaagctcga cttgacttat ttgaacgtct taatacaggt agcaaaaatg ctacacctgc
    42781 agaaattcgt cgaggtctgc ttcaaggagc attttacgat atggttgaga ctttatctga
    42841 agataaaaca tttaatgaat tagcaaaaat tagcaagaat atgcaggata tgggggagcg
    42901 agaagaattc attactcgct tttttgccta tggtgagtat ttagaccatt attctgctaa
    42961 tgttcgtaaa tttatctttg attatacaga aatcatgaat gaagaatttg ctaaaaatcc
    43021 aaacatgcgt gcgacttacg aacaaaaatt ccatgaaatt atggcttttt tcaaagaaaa
    43081 atatccaatt ggttttaaaa gagatggaaa gggtcagtct tctcgtactc gtttcgaagg
    43141 catggctgtt tcagcttatc tcgctgaaaa ggaaaatcca ggcattttag ctcaagttac
    43201 cccaaaggag tgcgttgata tttttgacag taaagaattc attgaagtaa ctggtagtga
    43261 tggtgcaaat gccactaaaa agctacatgc tagattattt tttgctaaaa atgcctttaa
    43321 acggaatcat ccatgaactc agtaaaatct atgatggaaa gtcgtgaaga ggaaataaac
    43381 ctatatatgg atctgctaaa gtatatccat gatagtactc aacatggttt accagcttta
    43441 caaaatagaa atggtgttga atatcaattg gaaaatactc atctccatat ccttaccgct
    43501 agtatctata tgttactata taactacatt gaggctgttg ttacccaaag cctaaatact
    43561 ttaatttgct atattcaaaa tgaaaggatg cctattaccc aattttcaga acccatccaa
    43621 aaagaatggc taataggagg gttaaacctt aatgatgcaa aagctagtgt agaaacacga
    43681 ttaggtaatg gactcaaaat gcttaaacat ttcggtgata cacaaaaaat agatattaag
    43741 atttctaaag gaggaggaaa ttttaatgat gacgaaatat ttaagcttgc aaagaaactt
    43801 caagttaata tgtgtatcga tgacgaaact caccgccaat tacggcaagc aggaaatatt
    43861 acctatgaaa gtacgcgcaa atttcaagat acttgtatga aagaaataac ccgtgaacga
    43921 aataaacttg ctcatggaga aatcaccttt caagaatgtg gtagaaaccg aacaatatct
    43981 gatttaatgg caataaaaga cactactttc cgctatatgc atttatttac tgatgcattt
    44041 actgaatatc taatgcagaa gaaatatctc aaaccaacag tttaaaacta tttccgattg
    44101 cctcacctag caatacagga acagcattac caatcattcg actaatttgg cgaattggag
    44161 tatcagtttc ttgagtgaac tggtaatcta gaggaaaact ttggaataag gctgcctccc
    44221 ttaaagaaat tgcacggtct tgttccggat gcccaaagcg tccattgccg aagccaatac
    44281 ataaagttgt cattgttggg gcgggcttgt cccattccat acgaccataa attcgtttat
    44341 aagactgtcc tttttttttg cgatgacatg cggctcgtaa atcttctggc caatccatcc
    44401 atgttcctcc tggacgagaa gcacgaatcc gttctagatt acgagctgac attgatgaac
    44461 accgatgtaa tacatctata gtatcttgtt tgcctgctgc cagtggcggc aagtgtccaa
    44521 tagcctgacg aactgttaca tggcaatttg aatgcgttgg aggaaccatc tgaatattac
    44581 cgatacgtga tgcaagtaat acgtgtctac gtcgtgattg agggactcca taatctgcac
    44641 ataatacttt ttcagcccaa acttggtatc cctgttgttt taaattattt acaaaatctt
    44701 ggtatacctc atgttttaca acatctggca cattctccat agtcacaagc tctggttgca
    44761 cttcatgaat caagcgagaa aaagcgtata gtaatggcca ttttttgtca ttacgaacat
    44821 cacgaccttg attgtaagta gaaaatggct gacaaggagc acaaccagct aataatctaa
    44881 ctgcatcttt tgaatagtgc gcctgaatct gatctccagt aactgtttcc acatcttgta
    44941 atataaattt tgcaccttta ttgttatact cgtacgcata tgcacaagct gcatcaatat
    45001 catatccagc tttcacttta attcctgctc gaagtaaacc tgctgtcaac ccacctgctc
    45061 cacaaaataa atctactgct tcaattttca tttttcaact catatttttg atataaggat
    45121 tatatcctta actttgattg gagtcgagct tttaatagta tataagctct gaatagtaca
    45181 gttccttatg aacgtttcta ttcattttta aaattatttc ctgaaaactt tgggggcaat
    45241 cattaaaatc cttcaataaa tggttataac ggacggcttt atcttgccat gctttaaata
    45301 gattttttgg aaaattttgt attaaccaca aacaagcatg aatagtactg tgtcgttccg
    45361 ccaaactgcg actttcccaa ctcagtcgac gagaacgttc taattgaact ggaatctgat
    45421 gtatttgctt gcacaaataa gagtacaaat ccggtgcaag cttctttgat gtagtaatcg
    45481 ccaaaaagtg atgcagaatt tttagttgtt ctttttcaga ttggttaaaa agataatcaa
    45541 gattagcaat ttggtttaac caattttgcc attgcagcct gatttcatca ttaaagaaaa
    45601 taacattctc acaaggactg ttgctcaagt catattgaca attccaacaa tatttcaact
    45661 ctgtaaagcg ctgcaaatta ggtttaccta aatcttgtcg gtgaaaagca atatatatgc
    45721 cacatttagg acagcaatca tgcatcatga tttgatgtat tggacaaaat gtatataggg
    45781 ctatgcgcca tgattttcgg aaatatgttt catggtcagt agctagacat agcgggcaat
    45841 aaacaatagc attattatcg cgtttacggt gattggcttt aaggctgttt atccacgtca
    45901 attgtcctga aaaattgttt ttatcaaata atcgaccttg taggctatct aatgtggttg
    45961 ttttgattcg aatggttgaa cagcctgttt tttcagccaa tgtattgacc aaccagtcag
    46021 gagtatggcg atctatatcc ctattccata tctcataatt aggaaatagc aaatgacaga
    46081 aggtttgtac tttcaacccg tttgcatgtg ctaagcgaac caaccatgat gtaaacaatt
    46141 catcaggata aggtttcgga tgagcgggta aaatcatctg agtttatcca attgacgttt
    46201 acgttggctt ggactaatcc agttgattga atttaagatt gtttcgttaa tacattcagt
    46261 ttgttgttga atggcggcta cagcagcttg tgtcagcaaa cgagaaagct ctccaatata
    46321 tccttcactc atgccatata actttgtagc caacgaaatt tcatgtaaat ccgacggctg
    46381 actaagcggc agcatccgtt caaagctcat caataaacgc ataaaatcct tatttaattc
    46441 ccaacgagga agcaccgcag gttcaaaacg gttggccaac tgtgcatcag tttgaatggc
    46501 tcgatatgcg tctttaatac ctacacctac aatcggaatt tgtaattcat ttcctaagta
    46561 tttaattaca tttaaaaacg ttttctgctt attcaaactc ccggaaagga tatggtggat
    46621 ttcatcaata atcaacattt tagtattaac gtatttgagc aaatgaagca cttgggcaag
    46681 ttttttatct gtcctgtcat gagttttata gggggcaaac agtaaatcta aaatagcatt
    46741 gtaaaaacgg ctttcatcag gtataggcgg tgcttgaata atcactactg gtgcgataat
    46801 accatttcca tgtggattat catgggctgg atgttgccgc aaaaaatgtt taatcaacat
    46861 ggttttaccg ttattagtat caccaaccaa caacatatta ggcatacggt tttgtttcgg
    46921 gaaacttagc aaatcttcca gttttaccaa aacctgttga gcctgcggat aacctatcca
    46981 acgaggggaa cgtatgtatt cgatgcgctc aatatcgcta tactttagtt gttcttttgc
    47041 tttgggagaa aggtgaggat aattcatgat aaatcgtcca aatcatcaaa aggctgaata
    47101 tcatcaaaca tattttcctc attttcagac gatataatat tcctcgaatc aatttgaggc
    47161 ataggtaaag atgctcgagt acgttgatct aattggcgac gttgttgagc gcgacggata
    47221 gctttagtct taccttgagc ttgctgttcc aattctcgca ttcgtccgta tgctgtaaaa
    47281 atcatgttct catcaatatt ctgtttctgc tcatttttta attgtctctg aatctctcgt
    47341 aactcccaaa tgctgataac tggattcgaa ctgttccgat aaggtattgg aaaatacatc
    47401 gataaatctg ggtcataaaa ccaaatcacg cttaaatcac gtggatctat gcgaaaaatg
    47461 aactggcgag cctgtctagg attagtcgga ttagttgctg caatccatgg tcgtaaaacg
    47521 tcatgccaat aagtgatttt atggataact accccatatt gctgcaccgt tcgcatttca
    47581 aacggcataa aggtcaagcg caatagtgtc tcatcaacca ctttatcagg taatcctgtt
    47641 cctaaagaaa catcatctcc taatacacct gatttccatt tggctaacgg tgagatcccc
    47701 aatccagaat gagtacgctg atgataaaca tcacaaataa atagtgttaa ccacgtttca
    47761 aattcagtta gcgttaatgc cgccttgctt tcggaagcat aatcttttct ctgttgtgta
    47821 tttgcaaaag tagtaccagt taaaccgtgg acttcatcca aaatcgtacc tagtaaacgc
    47881 tcaatgtgtc ctccgaaatg aggacgtgcc acaggcctcc actcaatttc aataccgtat
    47941 tgtttacacg ccttttccag cactttgcca cggaattctt ttgcattatc caaatgaatt
    48001 ttgcgtggca aaccccacac aggccaccgc cctttcactc catgttttgc caaccagctt
    48061 tccttagaca aaatactatg tgctaaacac aaacctgtag ataatgcact tggtggatcg
    48121 aatgacacat aaaatcccgt aaccattcga ctgaacacat caatcgccag cgtaatccac
    48181 ggcctaccaa taggcttacg atatatatcg tcaactaaaa taatgtctaa ctttgtatga
    48241 tctatttgta ccactgaaag cggataatcg gcatttggaa agctaccttg taaaggagaa
    48301 aatttcattt ctgctgcttt atggcttagt cgtttagcaa ttttaatttg aggcgcaata
    48361 tttttgattc gagaacgaat ggtattagca tgaggagctg gtaaattttg tttatggcaa
    48421 cgccgtgtaa tttctctaat gattttctgt attgatttac gttgtgtgct taaataatcc
    48481 acttccaata cttgcctgat aagctgttct acatcatcag aaagctgtgt tttaccacta
    48541 tcactacgct gcttacgtgt taaagcagtc agaacgccgc tttccttata actttttaac
    48601 cagcgataaa ccgtactgat atgtacaccg cattcggcag ctctcgcttg aacatcagat
    48661 ttactacgtt ttggatttcg gataagtggc tcaattacat cgaaatgttg ttgagctaat
    48721 tgccattttt gctcaggaat actatctaag tgggttaatt cagcactctg gactgttgat
    48781 agatccgcta attttgcttg gtaatctgca ccggttacac tatcgcgtaa ctgatatgtt
    48841 tctaactcca ggactgctgt aattacacag attttgtttt gaaacaaaac cattgcaccg
    48901 acattcacat ttaaagttgc tttcattttt taaaccatat agcggattgc atagtgagtg
    48961 gttgatttaa atctgcgcca atttttcgtt ctgcaattaa gtaccacaat acaggcaata
    49021 attttgcttg attgtattca tcctgaaaag tctgttgaat aagctgttta ggtgtggatt
    49081 ttttcaattc gcgtaacgat tcatccaata aagtcatata tgcttcgctg tattcgccag
    49141 ataagccatt ttgttgatat ggcaataaaa agcgtacatt gcataaataa tcagtatgaa
    49201 tctctaactc tgtaatcagt ttaaaatgcc aaccgtgtaa tttgcaaaaa tgtaaggcag
    49261 cctgaaattt aggtttgagt tcacaccagt ttttacgtaa atcgctacga tattttactt
    49321 cgtaaataat tacatcattt tgataatgaa ccaaaacatc aggggtatag atatgcttct
    49381 ttcctgctgg gtcaaaccac tcaatcgtga taggctgtac ttcgaaatgc tgcacattag
    49441 aatcaaattc cagcagagtt aaaaaaatca cgctccaaag atgactcata agctgcaatg
    49501 cctatagctt tgttatatgg agcgatgcct gtaatatttc gatagttttt aggaatttta
    49561 cggattggca tagttttata tgtgtagtag aaaaaggatt aaaaggaaga caatcttaat
    49621 gagttaatgc cctttatcaa taacccaaac tcaattagct ttcttataat ttagtctcaa
    49681 ataacaatat tattttcaaa tcttaatgca aaatttttgc tctcagtcgc aaataacgtt
    49741 attagaagta taagattttg catattctag tcgcaaacaa cgttggaatt cacagcgaaa
    49801 gccacggcgg ttgcgggcat cattaaaccc ggaacaccgc gccaaagtga agaacgattc
    49861 cctgcgcctg ccgcgccgtt tgatgtaaac aacatcaatt tgaattgtcc atgccgtctg
    49921 aaagcccttc agacggcatg atttattccc aaaatccgat aatctcaaaa gaatctgtca
    49981 gttaatgaga acgattcatt ataaattttg ataaaaaacc gtgctctatt tgttttttgc
    50041 tatggttaat aagtgtaaat gttctttatt gatgagatgg attcttagtt ttagcagcaa
    50101 aaaaaaacga taagaaacag agcattgatt tggaaggcat cggggaatac gccctatttc
    50161 ttttgataat tgtttgtatt ttaaatagaa aatttgtcat gccgacagct tttgcataca
    50221 atgcacgcat cccgaacagt gcttcatcca ctgtttaacc acacgaaacc caaattaaga
    50281 aaagagaaat attatgcaag gcaatcaagc agttttagat tacatgaacg aattgctgtc
    50341 cggtgagctg gcagcacgcg accaatactt catccactcc cgtctctact ccgaatgggg
    50401 ctacaccaaa ctttttgaac gtctcaatca cgagatggaa gaagaaacca cacacgccga
    50461 agactttatc cgccgcatcc tgatgctggg cggtacgccg aaaatggtac gcgccgaact
    50521 gaatatcggc acggacgtgg tttcctgcct caaagccgac ctgcaaaccg aatatgaagt
    50581 acgtgatgcg ttgaaaaaag gcatcaaact gtgcgaagag gctcaagact atgttacgcg
    50641 cgacctgatg gttgcccaac tgaaagatac cgaagaagac cacgcacact ggttggaaca
    50701 gcagctgcgc ctgatcgagc tgattggcga aggcaactac taccaaagcc aactgtaagc
    50761 tgtattcgga ataaaaggag tccgtatgaa aggcgaccgt ctggttatcc gcgagctaaa
    50821 caaaaactta ggcttgctgc tgataaccat caaccaatat ttccttcacg cccgtatttt
    50881 gaaaaactgg ggctttaaag aactgggcga acatttcttc aaacagtcca tcgttgaaat
    50941 gaaagctgca gacgatttga tcgagcgcat tctgttcctc gaaggcctgc cgaacctgca
    51001 agaactgggc aaactgttga ttggtgaatc taccgaagaa atcattgcct gcgatttgac
    51061 caaagaacag gaaaaacacg aagccctgct tgctgccatc gctacagcag aagcgcaaca
    51121 ggattacgtc agccgtgatt tgttggaaaa acaaaaagac accaacgaag aacacatcga
    51181 ctggcttgaa acccagcagg aactgatcgg caaaattggt ttgccgaact acctgcaaac
    51241 agcggcgcaa gaggactaaa acacaaacca ctgccaaata tagcagtccc ctgcatcatc
    51301 agacggcata tccgtataaa cgggtatgcc gtttttcttt gccggtttgc gaatcaaatc
    51361 taaattgatt tgaatcaata ctcgataacg gtttttgttt aataataccg atatgaaatc
    51421 tagattgctt gtttgctgtt tcccaatctg tcttgatttt acctctttcc tcttgatgtg
    51481 tgtgtgtgtt tgggtgtggc tgccgccacc cctttttttt ggcttttatg tgaagtaaaa
    51541 tccgtaacag caaatgctta actttaacat tcatccggaa gcaaatgatg ggcaacaaat
    51601 tgactctgcc tgccgaactg cctgccgaac aggatttgcg ggcggtattg gcatacaata
    51661 tgcggctttt ccgcgtgaac aagggttggt cgcaagaaga attggcgcgg caatgcggtt
    51721 tggacaggac ttatgtgtcg gcagtcgaac gcaaacgctg gaacatcgcc ctgtcgaata
    51781 tcgaaaaaat ggcggcggct ttgggcgtgg cggcgtatca gttgctgctg ccgccgcagg
    51841 aacggttgaa cctgatgacc aattccgccg atacccgaca aatgccgtct gaaagcggta
    51901 tttgaccgcc gaaccatccg tctccttttt tcccgccaat cgaaagaata aaaatgcctg
    51961 caaacctgtc ttcagcactg gaaaccttca aacagcagcg cgatgccgcc gaagcgcatt
    52021 atttgaaagc caagcgcgtg tcggtatttt tcagagaata cacggcggca gtcgaaacct
    52081 tactggcggc attgtgggta gaacattttc aaaacagcgc gttatgcctg atggcggtgg
    52141 gcggcttcgg gcgcggcgaa ctgtatccct gttcggatgt ggatttggcg gttgtctccc
    52201 ctgccccgct ttcagacggc attcaggaac agattgcccg gtttgttcaa accctatggg
    52261 actgcaaact gatgccgtct gtaaaaagcg gcggcattgg cgaactgtgc gaaagcgtgc
    52321 gcgacgatat tacgggggac acggcgtttt tagaggctag gtttttgttt ggcaaccgcc
    52381 aaacggcgga taaactagcg gaaaaaatga acgcgcagcg caatgtggcg gcgtttgtcg
    52441 aggcaaaact ggtggagatg gaacaccgcc acgccaaatc gcaaggctca ggggcggtat
    52501 tggaaccaaa catcaaaagc tgtccgggcg gtctgcgcga catccacacc ctgctttgga
    52561 tagcgaaggc gcaaggcttg gcgaccgacc tgcccgatct gctcaaacag cggattttga
    52621 cgcgtgccga agccggtatg ctttcgcacg gctaccgccg cctcgcccac atccgcatcc
    52681 atctgcattt aaacgccaat cgcgccgaag accgcctgct gttcgatttg cagccgcaag
    52741 tcgccgaaag catgggttat gaaggcttga acctccgccg ccaaagcgaa gaactgatgc
    52801 gcgtgtttta ccgcgcgatt aaaaccgtca aacaactggg cggcatcctc acgcctatgc
    52861 tgcaaagccg cgtttcctcc accccgttgc gcgttaccct gcggattgac gacgactaca
    52921 tccaagtcaa caaccaaatc gccgcgcggc acaccgatat ttttttcaga cggcccgaac
    52981 acattttcaa aatcgtcgaa atcatgcagc agcgcaacga tattaccgcg ctcgaaccgc
    53041 aaaccctgcg cgcatggtgg ggcgcgacgc gcaaaatcaa ccgcagcttc taccaaaatt
    53101 ctgaaaaccg ccaccgcttc gccggctttt tccgcaacgg caacggactg acccaaaccc
    53161 tgcgcttcct caacctctac ggcgtattgg gacgctacct gcccgcgtgg gaaaaaatcg
    53221 tcggcctgct ccagcacgac ctgttccaca tttatcccgt ggacgaccac atcctcgccg
    53281 tcgtccgcaa cgtccgccgc cttgccttgg atatgcacag ccacgagctg ccctatgcct
    53341 ccgcgctgat gcagtccttt gaaaaacaag acatcctcta tctcgccgcc ttcttccacg
    53401 acatcgccaa aggacgcggc ggcgaccacg ccatacaagg catcgcagac gcgcgccaat
    53461 ttgccgctga ccacttcctg accggagaag aaagcgacct gctcgcctgg ctggtcgaaa
    53521 accacctgct gatgtctgcc gtcgcccaaa aagaagacat ccaagacccc gacgtactcg
    53581 atgctttctg caaacgcgtc caaacccacg aacgcctcag cgcgctctac cttctgacca
    53641 tttccgacat acgcggcacc aatcccaagc tgtggaacgc atggcgcgcc agcctgctgg
    53701 aaagcctctt ccatgccgcc ggacgctacc ttacaggcaa cggcggcaac ccgcacaccc
    53761 tcttcggccg ccgccggcag gaagccgccg acttactcac ccgcgccgcc gtccccgaaa
    53821 aacagcagaa aaaactatgg aacgcgctcg gttccgccta cttcgcccgc caccagtccc
    53881 gcgaaatcct gtggcacgcc gccaacctag tccacgactt tgaaaccccc atcgtccgca
    53941 gccgcatcct gcccaaaagc gacagctttc aagtcatggt tttcatgccc aacggcccgc
    54001 gcctgttcgc ccgcctctgc cgcatcttca gccgccacgg cttcgatatc ctcgccgccc
    54061 gcgcctttat taccgaacac gactacatcc tcgacacctt catcgtgcaa atcccctcgc
    54121 agcacgctcc cgaagactac cccgacatcc aaagcgcgct cgaagccgaa ctcaacagct
    54181 ttatccacgg acacaccgtt gccgaaatct ccagccatag ccgccgcatc agccgccgca
    54241 gccgctacat gccgatcgcg ccaagcatca ccatcacccc cgaagaagac tatccggact
    54301 ggtactccgt cgaaattacc gccgtcaacc gccccttcct gctcgccgat atggcggaag
    54361 tctttttcgc ccacaacgtc agcctgcgct atgccaaaat ctccacactg gatgaacgcg
    54421 ccgaagacag ttttaccgtt tttagccccg acctgaaaaa cccaaaaatc cagtcctcat
    54481 tgaagcaggc tttgctggaa cagttgacat aagcaccctg ccggttttca gacggtatcc
    54541 cgccgccgcg ccgtacgctg tctgaaggcg ggggatattg caaacaatat aaactgtcaa
    54601 gccgccggta acgtgctata atgccgtgca tcttgcacaa actgaaagac cgagcattat
    54661 gcgtatcgta gaaaaagcct atactttcga cgatgttttg ttggttcccg cacattcgac
    54721 cgtgctgccg cgagacgtta aacttcaaac caagctcacc cgcgaaatca tactcaacct
    54781 ccccctgctt tccgccgcga tggacactgt taccgaggcg cgcctcgcca tttcgatggc
    54841 acaggaaggc ggcatcggca tcatccataa aaatatgccg cccgaaatgc aggcgcgcgc
    54901 cgtttccaaa gtgaaacgcc acgaaagcgg cgtggtcaaa gaccccgtaa ccgttgcgcc
    54961 gacaacgctc atccgcgaag tcttggaaat gcgtgcgcag cgcaaacgca aaatgtccgg
    55021 cctgcccgtc gttgaaaacg gcaaagtcgt cggcatcgta accaaccgcg acctgcgttt
    55081 tgaaaaccgc gtcgatttgc ccgtttccgc cattatgacc ccgcgcgaac gtctggttac
    55141 cgtccccgaa ggcacaagca tagacgaagc gcgcgaactg atgcacacgc acaaagtcga
    55201 gcgcgttttg gttctgaacg aaaaagacga actcaaaggt ctgattaccg tcaaagatat
    55261 tttaaaaacc accgagtttc ccaacgccaa caaagactcc gaaggccgtc tgcgcgtcgg
    55321 tgcggcagtc ggcaccggcg gcgataccga agagcgcgtc aaagccttgg ttgaggccgg
    55381 cgtggacgtg attgtcgtcg ataccgccca cgggcacagc caaggcgtga ttgatcgcgt
    55441 tcgctgggtc aaagaaacct atccgcacat ccaagtcatc ggcggcaaca tcgccactgc
    55501 caaagccgca ttggatttgg tcgccgccgg cgcggatgcc gtcaaagtcg gtatcggtcc
    55561 gggatcgatt tgcaccaccc gtatcgtggc aggcgtgggc gtgccgcaac tgaccgccat
    55621 tcacaacgtt gccgaagccc tcaaaggcac gggcgttccg ctgattgccg acggcggcat
    55681 ccgcttctcc ggcgacatcg ccaaagccct cgccgcaggc gcgtacagcg tcatgctcgg
    55741 cggtatgttt gcaggcacgg aagaagcgcc gggcgaaatc gaactctacc aaggccgctc
    55801 atacaaatcc tatcgcggta tgggttcctt gggcgcgatg agccaaggtt ctgccgaccg
    55861 ctacttccaa gacaaaaccg acagcaccga caaatacgtc cccgaaggca tcgaaggccg
    55921 cgtgccttac aaaggcccga tcgtgaatat catccaccaa ctgaccggcg gtctgcgttc
    55981 cagtatgggt tacttgggtt gcgccaatat tgccgaaatg cacgaaaaag ccgaatttgt
    56041 ggaaatcact tccgcaggta tgagcgaatc gcacgttcac gacgttcaaa tcactaaaga
    56101 agcaccgaac taccaccgct gatttgaaca gcctttcagg acaaaatgcc gtctgaagcc
    56161 ctgttttcag gtttcagacg gcattttttg tccgttcaag gtaagccgca cgggctgatt
    56221 gcgcggacgg gcaaagcaat tcggacggac acgccgaaag aatgaaaatg ccgtctgaac
    56281 gccgtttgcg ccccgtctgc cgaataccgc cggcctttaa aacggcggtg gaaataaacg
    56341 caatctttca atacgggcgt ttcgattggc ctgaaatgat gcgccggccg ccatatcaaa
    56401 aaatcgggcg gcggcttcgg gaaagtatgc cccgccctcc ccaagccacc ccgtcagcat
    56461 tatgatttgc ctttccgttt ttttacctta atcggaaccg gtttctttgc ctttttcccc
    56521 gattctgcgg cggcagacgc gccccttccc cttactttgc cgcctcgggc tgtttttttc
    56581 ccggcggcgg ttttcggctt ccctttcccc gccgtccctg ccggtttggc agacgcggat
    56641 gatttaactt tccgccctct gccgctcccc tcggcaatca ggacaaaatc gatttttcca
    56701 tcatccaaat cggcacgggc cactttaatg gcaaccctgt cgcccatatt gaaacggatg
    56761 ccactgcgtt caccttcgat tgccatgatt tcggggcgga agttgaaata gtcttcgccc
    56821 aaatcgctga tatgcaccag accgtcgata tggatatcgt ccaaagtgac aaatattcca
    56881 aaatttgcca ccccggaaat tttgccttca aatatttcgc cgactttgtc acgcatatag
    56941 taggttttca gccagttttc cacgtcgcgg ctggcgtcgt cggcacggcg ctcacagaac
    57001 gaggtatgca cgcccaaagc ctgccagctt ttttttggcg tgtaggtttg ctgattcaac
    57061 acggctttga tggcgcggtg tacggtcagg tcgggatagc ggcggatggg cgaggtgaag
    57121 tgggcgtatg cttcgtaggc aagaccaaag tgtccgtcgc aatgcggttc gtaaaccgcc
    57181 tgttgcatgg agcgcaacat catgacttgc agcaattcgg catccggcct gcctttgaac
    57241 tgtccggcaa gcgcggcata gtctttcggc gacgggttgt cgccgccgcc aagttgaagc
    57301 cccaacagac cgaactgctc gcgcaaggcg gcgagttttt cgggcgtggg ccccaaatgg
    57361 ttgcggaaca atgcggtatg cttgtttttc aacagaaaat ccgctgcgca aacgtttgcc
    57421 gccaacatac attcttcaat cagcttgtgg gcatcgttgc ggacaacggg gacgattttt
    57481 tcaattttac cgttgtcgtc gaaaagcatt tgggtttcga tgctgtcaaa ctccaccgcc
    57541 ccgcgttcga aacgcttttt ctgaaggatt ttgaagagtt tgtaaagcgt gtcgatttgg
    57601 gctttgaacg gatgcccgat gccgcctgaa agccatttcc aaacttggtt gtaggtcagg
    57661 cgggcatgag agcgcatcac ggcggggtag aagcggtatt ctttaatatt gcccgcgtaa
    57721 gtgataacca tatcgcacac catacacaaa cgctcgacat taggattgag cgagcagatg
    57781 ccgttggaca ggttttccgg caacatggga atcacgcggc gcgggaagta aacactggtg
    57841 ctgcgttcct gagcgtccgt gtcgatagcg tcatcggggc ggacataatg gctgacatcg
    57901 gcaatcgcca cgaccagacg gtaattgcgt ccgatttttt cggcaaacac cgcatcgtca
    57961 aaatctcgag ccgtttcgcc gtcaatcgtt accaaaggca ggtcgcgcaa atcgacgcgg
    58021 cctttcaaat cgcttttgcg tacatggtcg ggaatttttt tcacggcttt ggcacacgct
    58081 tcactgaatt ggtgcggcaa atgatgcttg cgcacggcaa tttcaatctc catgccgctg
    58141 tcggcataat cgcccaaaac ttcgatgatt tttgccactg ccggccggtt ttgctcagga
    58201 taaacctcaa tttcgccgac gatgacctga ccggattcag gtttgaaacg cgccacgccg
    58261 tccggttcca atacgatgct ttggttcaga cgcttgtctt ccggctccaa aatcgccacg
    58321 cccctatcca tatagaaacg gccgaccact ttgctttgcg cgcgttcgac aatatccaga
    58381 actgtccctt cgcggcggcc cctacggtcc atgccggcag gacgaacagt gacaatatcg
    58441 ccgtgcataa tgccgcgcat ctggcgttcg tacaaaacaa aatcaccgtc tttggcgggc
    58501 gtgagcggca cggcgaaacc gaagccgtct ttgtgcgcct cgacacggca tttgaccaaa
    58561 tccaatttgt ccgccgcgca aaccgcgccc cggcggttga ttaaaacctg accgtcccgc
    58621 gccatcgcct tcagacggcg ttcgaaaaag acatactcgt cttccgtaat cgacagctcg
    58681 cgcgcaagcg attcgatttt tgaaggcaca cctttgcgct ccaacaattc gattatccat
    58741 tcccgactgg gcaaaggatg ttcataacgc tgtttttcac gacttaaaaa cgggtctttt
    58801 tcccgtaaat ttaaagattt aatattttta ttcatttgga ctgttgacat tctctttatg
    58861 aactatataa tagcgacttc tttgcggcag gacactgtac cgcaaaacag caaataaagc
    58921 aaagcccggg tggcggaatt ggtagacgcg ctagcttcag gtgctagtat cctcacgggt
    58981 gtggaagttc gagtcttctc ccgggcacca aatcaaatgc tttgctttat ttcaatattt
    59041 agattgcccg ggtggcggaa ttggtagacg cgccagcttc aggtgctggt atcctcacgg
    59101 gtgtggaagt tcgagtcttc tcccgggcac caaataataa attatcccga ttgggataat
    59161 ttccgccatt agagaggtgg atgagtggtt taagtcgcac gcctggaaag cgtgtatacg
    59221 tgaatagcgt atcgagggtt cgaatccctt cctctctgcc aaatacaaat cccaaacttt
    59281 tacagtttgg gattttttgt tattcaaaca ataatttcat tgggaaaacc tgttattgtc
    59341 tataatcgtc atcaaccgtt tacaactatc cgttaccgtt tttttatggt acgttttatc
    59401 ctcttcctca gcctgtaagg actcaaaagg cgtatccctt caaacggcat tattcgacat
    59461 gctgccggga agccgaaccc gataccgaaa cgcgacgggg cgatatgccg ctggaaaaat
    59521 ggcggcagtt tcttgattgg gtaggaaaaa cg
//
LOCUS       DAXIEB010000003        38547 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000003 DAXIEB010000000
VERSION     DAXIEB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.003"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            39..1079
                     /gene="murB"
                     /locus_tag="ACFOA3_000145"
     CDS             39..1079
                     /gene="murB"
                     /locus_tag="ACFOA3_000145"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6897224.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1091..1741
                     /locus_tag="ACFOA3_000146"
     CDS             1091..1741
                     /locus_tag="ACFOA3_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6897225.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1796..2572
                     /locus_tag="ACFOA3_000147"
     CDS             1796..2572
                     /locus_tag="ACFOA3_000147"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6897226.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            2623..3189
                     /locus_tag="ACFOA3_000148"
     CDS             2623..3189
                     /locus_tag="ACFOA3_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6897227.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3209..4099
                     /locus_tag="ACFOA3_000149"
     CDS             3209..4099
                     /locus_tag="ACFOA3_000149"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6897228.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAISDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4124..4882
                     /locus_tag="ACFOA3_000150"
     CDS             4124..4882
                     /locus_tag="ACFOA3_000150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6897229.1"
                     /translation="MNPKISSDHTEDTIRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLSRLPPGKWRYLAPGESF"
     gene            complement(4937..5602)
                     /locus_tag="ACFOA3_000151"
     CDS             complement(4937..5602)
                     /locus_tag="ACFOA3_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6897230.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5715..6488)
                     /gene="folE2"
                     /locus_tag="ACFOA3_000152"
     CDS             complement(5715..6488)
                     /gene="folE2"
                     /locus_tag="ACFOA3_000152"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6897231.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            6691..7848
                     /locus_tag="ACFOA3_000153"
     CDS             6691..7848
                     /locus_tag="ACFOA3_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6897232.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGVAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKLGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHCQNSLEIARRLEAHPAIEKIFY
                     PGLPSHEHYELAKAQMPKGTGGVVTVYLKNDTREAANNVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKAGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(7911..8912)
                     /gene="hemB"
                     /locus_tag="ACFOA3_000154"
     CDS             complement(7911..8912)
                     /gene="hemB"
                     /locus_tag="ACFOA3_000154"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6897233.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTANKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGTIREALEDTGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9095..9379)
                     /gene="yhbY"
                     /locus_tag="ACFOA3_000155"
     CDS             complement(9095..9379)
                     /gene="yhbY"
                     /locus_tag="ACFOA3_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6897234.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEA"
     gene            9489..10109
                     /locus_tag="ACFOA3_000156"
     CDS             9489..10109
                     /locus_tag="ACFOA3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6897235.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10173..12140
                     /gene="ftsH"
                     /locus_tag="ACFOA3_000157"
     CDS             10173..12140
                     /gene="ftsH"
                     /locus_tag="ACFOA3_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6897236.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12361..12792
                     /locus_tag="ACFOA3_000158"
     CDS             12361..12792
                     /locus_tag="ACFOA3_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6897237.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            12785..13063
                     /locus_tag="ACFOA3_000159"
     CDS             12785..13063
                     /locus_tag="ACFOA3_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6897238.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13116..13694
                     /gene="pth"
                     /locus_tag="ACFOA3_000160"
     CDS             13116..13694
                     /gene="pth"
                     /locus_tag="ACFOA3_000160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6897239.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            13755..13961
                     /locus_tag="ACFOA3_000161"
     CDS             13755..13961
                     /locus_tag="ACFOA3_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6897240.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            13958..14320
                     /locus_tag="ACFOA3_000162"
     CDS             13958..14320
                     /locus_tag="ACFOA3_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6897241.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            14551..15966
                     /locus_tag="ACFOA3_000163"
     CDS             14551..15966
                     /locus_tag="ACFOA3_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6897242.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLMILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GLLLNILCVVLVALWAYAVLV"
     gene            16085..16594
                     /locus_tag="ACFOA3_000164"
     CDS             16085..16594
                     /locus_tag="ACFOA3_000164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6897243.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNEKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            16761..18143
                     /locus_tag="ACFOA3_000165"
     CDS             16761..18143
                     /locus_tag="ACFOA3_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6897244.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(18163..18918)
                     /locus_tag="ACFOA3_000166"
     CDS             complement(18163..18918)
                     /locus_tag="ACFOA3_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6897245.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(18928..19644)
                     /locus_tag="ACFOA3_000167"
     CDS             complement(18928..19644)
                     /locus_tag="ACFOA3_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6897246.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19634..20461)
                     /locus_tag="ACFOA3_000168"
     CDS             complement(19634..20461)
                     /locus_tag="ACFOA3_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6897247.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20746..21654
                     /locus_tag="ACFOA3_000169"
     CDS             20746..21654
                     /locus_tag="ACFOA3_000169"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6897248.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            21887..25501
                     /gene="recB"
                     /locus_tag="ACFOA3_000170"
     CDS             21887..25501
                     /gene="recB"
                     /locus_tag="ACFOA3_000170"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6897249.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQHNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAENNSNDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRLADNAPSGTDFAFTEDAPPPAVYRGNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            25540..25899
                     /locus_tag="ACFOA3_000171"
     CDS             25540..25899
                     /locus_tag="ACFOA3_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6897250.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            26024..26503
                     /locus_tag="ACFOA3_000172"
     CDS             26024..26503
                     /locus_tag="ACFOA3_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6897251.1"
                     /translation="MKHILPLIAASALCISTASAHPAGEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(26775..28154)
                     /gene="radA"
                     /locus_tag="ACFOA3_000173"
     CDS             complement(26775..28154)
                     /gene="radA"
                     /locus_tag="ACFOA3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6897252.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(28340..29404)
                     /gene="hemE"
                     /locus_tag="ACFOA3_000174"
     CDS             complement(28340..29404)
                     /gene="hemE"
                     /locus_tag="ACFOA3_000174"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6897253.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEAARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRHYHGG"
     gene            complement(29479..30696)
                     /locus_tag="ACFOA3_000175"
     CDS             complement(29479..30696)
                     /locus_tag="ACFOA3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6897254.1"
                     /translation="MKTVVWIVILFAAAVGLALALGIYTGDVYIVLGQTMLRINLHAF
                     VLGLLIAVVVWYFLFKFVIGILNIPEKMQRFSLARKGRKAALYLNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMGNTELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYETVEANLHAAAKMNAGLTRLVRLQLRYAFERGDALEVLAKTEKLSK
                     AGALGESETKQYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            complement(30693..32024)
                     /locus_tag="ACFOA3_000176"
     CDS             complement(30693..32024)
                     /locus_tag="ACFOA3_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6897255.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALATCALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPLKQAISSDLAELKNRPYVDISGT
                     ALRIDRLETAVSGLPLMLDGVLKPGVQARNEAVSASWWNVWEKSLGTLKGLVEIRRLE
                     NNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQSDLNNAETAVRQYFDAKSP
                     ATQSWLKELAELKALDVRMTADDGLKASLNAVRAYRDGTRMTAAENQEAEQAASEPAN
                     EKTASESAAASDVKTIEAPSLPSELKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            complement(32039..32800)
                     /locus_tag="ACFOA3_000177"
     CDS             complement(32039..32800)
                     /locus_tag="ACFOA3_000177"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6897256.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(32821..33240)
                     /locus_tag="ACFOA3_000178"
     CDS             complement(32821..33240)
                     /locus_tag="ACFOA3_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6897257.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFPCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(33491..33811)
                     /locus_tag="ACFOA3_000179"
     CDS             complement(33491..33811)
                     /locus_tag="ACFOA3_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6897258.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(33834..34460)
                     /gene="upp"
                     /locus_tag="ACFOA3_000180"
     CDS             complement(33834..34460)
                     /gene="upp"
                     /locus_tag="ACFOA3_000180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6897259.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(34566..34877)
                     /gene="grxD"
                     /locus_tag="ACFOA3_000181"
     CDS             complement(34566..34877)
                     /gene="grxD"
                     /locus_tag="ACFOA3_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6897260.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(34978..35193)
                     /locus_tag="ACFOA3_000182"
     CDS             complement(34978..35193)
                     /locus_tag="ACFOA3_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6897261.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(35219..35998)
                     /locus_tag="ACFOA3_000183"
     CDS             complement(35219..35998)
                     /locus_tag="ACFOA3_000183"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6897262.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYWLFKKVPDMRVV"
     gene            complement(36003..36353)
                     /locus_tag="ACFOA3_000184"
     CDS             complement(36003..36353)
                     /locus_tag="ACFOA3_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6897263.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(36357..37334)
                     /locus_tag="ACFOA3_000185"
     CDS             complement(36357..37334)
                     /locus_tag="ACFOA3_000185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6897264.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(37368..38498)
                     /locus_tag="ACFOA3_000186"
     CDS             complement(37368..38498)
                     /locus_tag="ACFOA3_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6897265.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
BASE COUNT         9119 a        11193 c         9739 g         8496 t
ORIGIN      
        1 atctaggaca gccccaaaca tattttaaag ttcaaaccat gcaacccatc cgataccgaa
       61 ccgacctcac cccctacaac accttcggtc ttcgcgccca agcccgagcc tttattgcgc
      121 ttgaacacgc cgacgggttg cgcgacatcg tccggctacc cgagttcaac cgagatactg
      181 ttttatggct gggcggcggc agcaatatcc ttttgatgga ggactacgcc ggtctggtcg
      241 tacatatgga aaacaaaggc atacgcgaaa ttgctcgttc agacggcatg gttctgattg
      301 aagcgcaggc gggcgaaatc tggcacgatt ttgtcctgca cgccgtcgcg ctgggtttga
      361 acggtttgga aaacctgagc ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca
      421 tcggcgcata cggcgtagag gcgaaagatg tgattcacag cgtgcgctgc ttcgatttgg
      481 atacggaaac ctttgtaacc ctttccaatg ccgactgccg cttcgcctac cgcgaaagcc
      541 tgttcaagca ggaaggtaaa gggcgttatg tgattgtttc ggtcgtattt gcattaaaaa
      601 cgcattttgt gccgaacttg ggttacggcg atttggcggc cgccgttgcc gaactgagcg
      661 cgggcagggt gccgacggcg aaagatgttt ccgatgcagt gtgtgcaatc cgcaacagta
      721 aacttcctaa tcctaacgtg cttggcaatg tcggcagttt ctttaaaaac cccgtcgtca
      781 gcgcagaaaa agccgccacc ttgttgcagc ggcatcctga tatgccgcgc tatccgcagc
      841 ccgacggttc ggtcaaactc gccgccggct ggctgatcga ccaatgtcgt ctgaaaggct
      901 ttcaaatcgg cggcgcggcg gtacatgaca ggcaggcttt ggtcttagtg aacaaaaaca
      961 atgcctcggc aaacgatgtc cggcagttgg cgcaacacat caaatttaca gtatttgctc
     1021 ggtttcaggt agaattgcac gccgaaccca attggctgcc tgcttcgttc agcctgtaaa
     1081 tccaaggagt atgcccgtga cccgccccgc caaaatcaat acttacacac gcatcatcga
     1141 cgccagcctt gcgcttttca acgaggaagg cgagcgcaac atcagcacca accatattgc
     1201 cgcccacttg ggcatcagtc cgggcaacct ctattaccac ttccgcaaca aagacgaaat
     1261 catcgtccaa ctgttcaaac gttacagcga agccctgctg gaatacctca acgaagccgt
     1321 gttgccgtct gatgtggaag actccatcaa ttatatggcg ggcatttacg acgtgatgtg
     1381 ggaataccgc ttcctattca gcgacgtgaa caccctgctt gcacgcagtg ccgaactatt
     1441 gggcgaacac aataccttca cccaagccaa agtctccccg ctcttggtca acctgctgac
     1501 ccagctcaac ggtctgaaca tcatccaagc cgaccaaacc gccatgaacg acctcgccgt
     1561 caatatgtgg atgatcacga aatactggtt cgacttcgac agctccctgc gcggccgcac
     1621 caagctgacc gaagactcca aagcacgcgg catccgccgc accttaagcc tccttcgtcc
     1681 ttatcttttg cccgaacacc gcgagaaata cgaccggaaa atcggcaacg gcaacccgta
     1741 aacccacagc gtccgaatca gccttcagac ggcacttacc cgacggtatc agcgaatgcg
     1801 cgcagtcccc ccgccccata tctccatcac aggcctaggt tacctcggcc tgccgctggc
     1861 acagaagttt taccaacacg gcagccgcgt cgccgccata aaacgcagcc tgacttcgga
     1921 cgacatcaac ctgcccatac atctcgacac cttcgacctc ggcagcaccg atgcctttca
     1981 gacggcattg tggcggcacc acgccgacaa acccgtctgg ttcttcctgc taccgccctc
     2041 ctcgctgacg cattacgccg acaccgtcaa acaatgggca gaacttgccc gggcgtgcaa
     2101 cgtgcaacac ctgattttca caagcagcac cagcgtttac ggcgatacag cgcgcgaatg
     2161 cgacgaaacc gccacacccg atccgcaaac cgagtccgcc cgccaaatcc tcgccgccga
     2221 acaacacctg ctcgacagcg gcatctccaa catcgacatc ctgcggctgg gcgggcttta
     2281 ttgcgccgaa cgccatcccg tcagccgcct tgttcaaaag caaaacatcc cgggcggcaa
     2341 ccgccccatc aacatcgtcc accgtaatat cgccgtcgaa agcctgtttc agacggcatt
     2401 taaccccggc ggcaggcggc tgaaaaacat tatcgaaccg cgccacccga cacgacgcga
     2461 attctacacg gaagaagccg ccaaactcgg tttgccggct cctgatttcg cacccgacga
     2521 cagcatgggc aaaatcatcc gtaccgtttg cgataacagc ttaagcctgt aaaataagtg
     2581 tcaacagcaa caaacaggca ttctccgccc cgatcagaaa atatgtccgc cctcctcccc
     2641 atcatcaacc gcctgattct gcaaagcccg gacagccgct cggaacttgc cgcctttgca
     2701 ggcaaaacac tgaccctgaa cattgccggg ctgaaactgg cgggacgcat cacggaagac
     2761 ggtttgctct cggcgggaaa cggctttgca gacaccgaaa ttaccttccg caacagcgcg
     2821 gtacagaaaa tcctccaagg cggcgaaccc ggggcgggcg acatcgggct cgaaggcgac
     2881 ctcatcctcg gcatcgcggt actgtccctg ctcggcagcc tgcgttcccg cgcatcggac
     2941 gaattggcac ggattttcgg cacgcaggca gacatcggca gccgtgccgc cgacatcgga
     3001 cacggcatca aacaaatcgg caggaacatc gccgaacaaa tcggcggatt ttcccgcgaa
     3061 tccgagtccg caaacatcgg caacgaagcc cttgccgact gcctcgacga aataagcaga
     3121 ctgcgcgacg gcgtggaacg cctcaacgaa cgcctcgacc ggctcgaacg cgacatttgg
     3181 atagactaac cttcagacgg catccgacat gaacagccct tttcacaaca tcggcatcgt
     3241 aacccgcccc aacacgcccg acatccaaga caccgcacac acgctgatta cctttttgaa
     3301 gcagcacggc tttaccgtct atctcgacga agtcggcata aaggaaggct gcatctatac
     3361 ccaagacacc gtcggctgcc atatcgtcaa caagaccgaa ctggggcaat actgcgacct
     3421 ggtcgccgtt ttaggcggag acggcacctt tctctccgtc gcccgcgaaa tcgccctgcg
     3481 cgccgttccg attatcggca tcaaccaagg gcatttgggc ttcctgaccc aaattccccg
     3541 cgaatatatg acggacaagc tattgcccgt tttagaagga aaataccttg ccgaagagcg
     3601 catcctgatt gaagccgcac tgatccgcga aggcaaaacc gccgaacgcg ccctcgccct
     3661 caacgatgcc gtcctctccc gtggcggtgc cggacagatg attgaatttg aagtcttcgt
     3721 caatcaggaa ttcgtctata cacaacgttc ggacgggctg attgtctcca cccccaccgg
     3781 atcgaccgcc tattcgcttg ccgccggcgg ccccatcatg caggcaggat tacatgcctt
     3841 cacactcgta cccatctgtc cgcaatccat gaccaaccgc cccatcgcca tttccgacac
     3901 gtccgaaatc gaaatcctcg ttacccaagg cggcgacgcg cgcgtccatt ttgacggtca
     3961 aacccatatc gacgtgcaaa acctcgaccg cattaccatc cgccgctacc gcaacccctt
     4021 acgcatccta cacccgaccg actaccaata tttcaaaacc ctgcgccaaa aactgcactg
     4081 gggcgagcaa ttagtctaag ccggccttta cccgaaagta tccatgaatc ccaaaatttc
     4141 ctccgaccat acagaagata ccatacgcct gtccaaacgc atggcgcaac tcgggctttg
     4201 ttcgcgccgc gaagccgacg gctatatcga acagggctgg gtaacggtca acggcaaaac
     4261 cgccgtactt ggtcagaaag tttcaccggc agaccgtatc gaactaaaca agaaagccca
     4321 cgaacagcag gcggcacgca tcaccatcct gctgaacaaa cccgtcggct atgtcagcgc
     4381 acaagcggaa aaaggctata aatccgccgc cgaactgatt acctctgaaa atcgctggga
     4441 aggcgacacc agccgcatcc gtttcgatcc gaaacacaaa atcggtcttg cccccgccgg
     4501 tagactcgat atcgattcag tcggattgct ggtgttgacg caggacggcc gtatcgccaa
     4561 gcagcttatc ggcgaaaaca gcggcagtga aaaagaatat ttggtgcgcg tgcgcggcaa
     4621 attggacgaa aaaggacttg ccttactgaa tcacggattg agtttggacg gtgagaaact
     4681 gcgtcccgcc aaagtagaat ggcaaaacga agaccaactg cgcttcgtgt tgaaacaggg
     4741 taaaaagcgg caaatccgcc gtatgtgcga actggtcggg ctgcgcgtcg tcgggctgaa
     4801 acgcatccgc atgggcaagg tcaaactcag caggctgcca cccggcaaat ggcgttatct
     4861 cgctcccggc gaatcgtttt aaataagcat taaaaaatgc cgtctgaaaa tccttgcgtt
     4921 ttcagacggc attgccttat gcccaaataa cggtttcttc caacggcctg cgcgctttcg
     4981 tggcgatttc ctgaacgcgg tagccgaatg tcgcggcaac ggacacgccc cattctgccg
     5041 catcgaacaa accgaactgc ccggacaata cgcgttccat atcggcatag ttgaaacctt
     5101 ccaccgggca ggaatcgata cctgccatcg ccgcacccgt catcatgttg gctaacgcga
     5161 tataggtctg gcggcaacac cagtcaaaca aggcgcgcga atcgtccaaa atcttgatgt
     5221 cgtcggcttg aaacgcctga tacctcgcca aagatttttc tacggcatcc ggttcggtaa
     5281 cgccgcgccg tttgaggctt tccaacataa acgggctgtc gaagcgggca tttttcttcg
     5341 ccaaaaacac caccaaatga ctggcggtat ccaaagcatc cgccataccc caagaaaacg
     5401 gcttgatggc ctgtcggatt tcggggtttt gaactacaac aaactgccaa ggctccgaac
     5461 cgaccgaact gggcgacaaa cgcccgagtt ctaaaataaa ctgaaaatcc tcggcactga
     5521 ttttgcgtgc cgcatcgtaa tgccggcacg atttgcggtt tttaaatgcg gataggacct
     5581 gctctctgtc taataccgtc atagtgcatc cttttattac ttgtattgca aattgatttc
     5641 aaaccgatac ggcggcagat tcgacaataa cgcccaaacg cctttcagac ggcatttcgc
     5701 catgacaatc ccgcctacgg atacgcgata taggcataag ccgaatggtt gtggatagat
     5761 tcgaaattct cactctcaac gacaaagctc ttgatgcgtt tgtcggcaat cagcgaagta
     5821 gcgacatcac gcaccatatc ttccacgaat ttcgggtttt cgtaggcttt ttcggtaaca
     5881 tatttttcat cggggcgttt gagcaggccg tagagttggc agctcgcctg cgcctccaca
     5941 taatcgatga cttcctcgat accgacttcg gcatcggcag tcaggctgac ggtaacatgc
     6001 gaacgctgat tatgcgcgcc atattgggaa atttctttgg aacacgggca aagcgaggtt
     6061 acgggaatca tgaccttcat actgtggccg tatgccccgt ctttaatttc acccgtgagg
     6121 cagacatcat aatccagtaa ggaccggata ccggaaaccg gcgcggtttt cttgcggaaa
     6181 aacggaaaag aaacgctgat tttgccggcg cgggaatcca aaagcgcgac catttcggca
     6241 gtcagcctgt gcaattgtgc gaaatccaag gcttcagaat gttgctccat caatgcgaca
     6301 aagcgcgaca tatgcgtacc cttctgctcg gcgggcaggt aaaccgtcat ggtcaggcgg
     6361 gcaactgtgg attgcgttcc ttcggcggtt ttcagggtaa tcgggaagcg caggtcttta
     6421 atgccgacct gattgatcgg caggttgcgt aaatctctgc tggattgcac gtctgcaatg
     6481 gcgttcatgc gttgtttgtc cttaatattc atataattgt tgagatgtgt gtattgtatg
     6541 acaggcagat gccgtctgaa aaaacgctgt tggctgcctg cctgcaaatg cgagattata
     6601 tcacttgctt ttggcggctg cattgattgt ttctattttc gggatagtga taaaatcccg
     6661 acttattcat atcttacaag gcagaaacag atgaaattcg ccacccaagc catccattcc
     6721 ggctacgatt gcgacgaaca caaccgcgcg ctgatgccgc cgatttatca aaacagtatg
     6781 tttgcgctgc acgaaatcgg cgaaaatgtg ccttaccgtt attcgcgcct gagcaacccg
     6841 acccgtcaga ttttagaaga caccgttgcc gatttggaac acggtgtggc aggttttgcg
     6901 ttttccagcg gtatggcggg aattgatgcc gtgtggcgta ctttcctgcg ccctggcgat
     6961 accattgtcg ccgtcgccga tatttacggc ggcgcttatg acttattggt cgatgtttat
     7021 caaaaattgg gggtgaacgt tgtttttgcc gatttgggca atccggataa tttggacgaa
     7081 ttgcttaaag cacacaaggt caaactggtt tggctggaaa cgccgtccaa tccgcttttg
     7141 cgcttggtag acatcaaagc acttgccgca aaagccaaag cggccggtgc gctggtcggc
     7201 atcgacaaca cctttgccac gccgtatctg caacagccct tggatatggg ctgtgatttt
     7261 gtgttccatt ccgctaccaa atatttgtgc ggccattccg acgtattgat gggcatcgtc
     7321 gttgccaaaa ccaaagagct ggcgcagcct ttgcacgata tgatggtgca taccggcgcg
     7381 gttgcaggcc cgatggactg ttggctggtg ttgcgcggca tcaaaacact ggctctgcgc
     7441 atgaacgccc attgccaaaa ctcactcgaa atcgcgcgcc gtttggaagc tcatcctgcc
     7501 attgaaaaaa tattctatcc cggcctgccg tcgcacgaac attacgaact tgccaaagcg
     7561 caaatgccca aaggcaccgg cggcgtggtt acggtttatc tcaaaaacga cacgcgtgaa
     7621 gcggccaata acgtgattaa aaacatgaaa ctggtcaaaa tggcttccag cctcggcggc
     7681 gtggaaagtt tggtcaacca ttgctattcc cagtcccaca gcggcgtgcc gcatgatgtg
     7741 aaaatggaaa tgggcatcaa agccggcctg ctgcgtttct ccatcggcat tgaagacgta
     7801 gatgatattt ggaatgatat ttccgccgca ctcgatacaa ctttgtaaac tgtaaaaatg
     7861 ccgtctgaaa ccatggtttc agacggcatt tcaattaacc cggccgaaaa tcaacgcttc
     7921 aacatctttg ccgcctcaat agcgtaatag gtcaaaatcc cgtccgcacc cgcacgtttg
     7981 aatgccagca ggctttccaa aaccactttg ccgccgtcca gccagccgtt ggcaatcgct
     8041 gcctgcaaca tcgcgtattc tcccgaaacc tgataggcat aagtcggtac gccgaactcg
     8101 tccttcacgc ggcggacaac gtccaaatac ggtaaaccgg gcttcaccat caccatatcc
     8161 gcaccttccc gaatatcgag cgccacttca ttcagcgcct catcggtatt tgcaggatcc
     8221 atctgatagg tctttttatc tgccttgccc aaattgcccg aactgcctac cgcatcacgg
     8281 aaaggaccat agaatgcaga agcatatttg gcggaatatg ccataatccg cgtatggata
     8341 tgtccggtat cctccaaagc ctcgcggatg gtgccgatac gcccatccat catatcggaa
     8401 ggagcaacga cctgcgcacc cgcctctgca tgacataaag cctgtttcac caagacttct
     8461 acggtttcat cattcatcac gtaaccgttt tcgtccgtca gtccgtcctg accgtgaacc
     8521 gtataaggat cgagcgcgac atccgtcata atgcccagtt cgggaaacct ctcgcgcaag
     8581 gctcggacgg ccgtcggcac cagtccttca gggttatacg cctcctgcgc acattccgtc
     8641 ttatttgccg taacgacagg gaacagtgcc agcatgggta ttcccaaatt tgacgcttcc
     8701 tccgccgtaa acagcaacct gtccaaactt tgacgcttca cgcccggcat agaaggcaca
     8761 tcctcctcgc gcgccgaccc ctccaatacg aacaccggat aaatcaaatc atcggcggtc
     8821 agcgtgtgtt cgcgcatcag gcggcgtgaa aaatcatccc tgcgcatacg gcgcatacgc
     8881 gaagccggaa cattgcggta aggaaactgc ataagccctc caatcatcct gtcaacaatt
     8941 caaaccatac ggaagccgcc gcaacttgca gacagcatcg ggcggcaatc aaaatccgtt
     9001 ttcaccggaa agggtttcgg cccgccgaaa tgccgcgccg aaggacggcg tcccgcccat
     9061 taaagaacaa tagcaaaaca accgcttcag gctgtcaggc ttcgatattc ttacgccaca
     9121 ataccaaaag tttcccgata tgctgaacag gttgcgcgtc aaccgcctca cataaggcag
     9181 tgcagatttc gatacgttcg gcacggtcgt cgccgaatac gcgcacttta atcagctcat
     9241 gcgccgtcaa tgccgcatcg gtttccttga tgaccgcgtc cgtcagaccc tgctgaccga
     9301 ccatcacaac aggatggaga tggtgcgcgc gcgctttcag ttccaaaatt tctttggtgt
     9361 tcaatttggt atccgtcatt ttcctactgc ttgaataaaa agaataatgc ggcattgtac
     9421 gcgatttggc acgacatcgg caaaattaac gtacaatacg cggtttccat tccgacccgc
     9481 gaaccactat ggctgtacgt tccaaatcct caaaagcgtg gctgcacgaa cacgtcaacg
     9541 accactacgt ccatatggcg caaaaagacg gctaccgcgc ccgtgccgca tacaaacttt
     9601 tggaaatcaa cgacaaagac aaattaatca aacccggcac ggtacttgcc gacttgggca
     9661 gcgcaccggg aagctggtcg caggttgccg ccaagctgac gggtacttcc ggagcagttt
     9721 tcgccttgga catcctgcct atggaagcca tagggggcgt ctccttcatt cagggcgact
     9781 tccgcgagaa cgacgtactg gcacaattcg aaaccttgtt ggacaaccgc ccgctcgacc
     9841 ttgtaatttg cgatatggcg cccaatatgt cgggaaacgc cgtaagcgat caggcacgca
     9901 gcttttatct gtgcgaactg gctttagact tcgcctcgca acacctgaaa accggcggca
     9961 gctttttggt caaagtcttt cagggtgcag gctatcagga atacatggca gccatgcgcg
    10021 aaattttcgg cacggtgcag acgcgcaaac ccgaagcctc gcgcaatcgc tccagtgaga
    10081 tttatttatt gggcaaaaat aaacgctgac aatacagacg gcgtgcttta caatcatttc
    10141 cgttttacac ctcaattatg gagccttgct aagtggggaa cacctttaaa tcaatccttg
    10201 tctgggtcgc cttgggtatc ggcctgatgg ctgcgttcaa cgctttagac ggtaaaaaag
    10261 aagacaacgg gcaaatcgaa tattctcagt tcatccaaca ggtcaacaac ggcgaagtat
    10321 ccggcgtcaa catcgaagga tccgtcgtca gcggctacct gattaagggc gagcgcaccg
    10381 acaaaagcac cttcttcacc aacgcgcctt tggacgacaa cctgattaaa acactgctcg
    10441 acaaaaacgt ccgtgtaaaa gtaacgccgg aagaaaaacc gagcgcgctg gctgccctgt
    10501 tttacagcct gctgcccgtc ctgctgctga ttggcgcgtg gttctacttt atgcgtatgc
    10561 agacgggcgg cggcggaaaa ggcggcgcat tctcattcgg caaaagccgc gcccgcctac
    10621 tggacaaaga tgccaacaaa gttacctttg ccgatgtcgc cggctgcgac gaagccaaag
    10681 aagaagtgca ggaaatcgtc gattacctca aagcgccgaa ccgctatcaa agcctgggcg
    10741 ggcgcgtgcc gcgcggcatc ctgctggcgg gcagcccggg tacgggtaag acgcttttgg
    10801 cgaaagcgat tgcaggcgaa gccggcgtgc cgttcttcag catttcaggt tccgactttg
    10861 tcgaaatgtt cgtcggtgtc ggtgcaagcc gcgtccgcga tatgttcgag caggcgaaga
    10921 aaaacgcccc ctgcatcatc tttatcgacg agattgacgc agtcggccgc caacgcggcg
    10981 caggtttggg cggcggcaat gatgagcgcg agcaaacatt aaaccaattg ttggttgaaa
    11041 tggacggttt tgagagcaat cagactgtaa ttgtgattgc ggcaaccaac cgccccgacg
    11101 tactcgatcc tgcgctgcaa cgccccggcc gtttcgaccg ccaagtggtt gtccccctgc
    11161 cggacatccg ggggcgcgaa cagattttga acgtccattc taaaaaagtg cctttggaca
    11221 aatctgtgga tttattgtcc ctcgcgcgcg gcacgccggg tttttccggc gcggatttgg
    11281 cgaacttggt caacgaagcc gccctgtttg ccggccgccg caataaagtc aaagtcgatc
    11341 agagcgattt tgaagacgcc aaagacaaaa tctatatggg tccggaacgc cgcagtatgg
    11401 tgatgcacga agacgaaaaa cgtgcgacgg cgtatcacga atccggacac gcgattgttg
    11461 ccgaaagcct gccctttacc gaccccgtcc acaaagtaac cattatgccg cgcggacgtg
    11521 cgctgggtct gacttggcag cttccggagc gcgaccgcat cagtatgtat aaagatcaga
    11581 tgttgagcca gctctccatc ctgttcggcg gacggattgc cgaagacatc ttcgtcggac
    11641 gcatctccac cggcgcatca aacgactttg aacgcgcaac ccaaatggcg cgcgaaatgg
    11701 taacgcgcta cggcatgagc gacaaaatgg gcgtgatggt ttatgcggaa aacgaaggcg
    11761 aagtcttctt gggacgcagc gtaacccgtt cgcaaaacat ttccgagaaa acccagcagg
    11821 acatcgacgc ggaaatccgc cggattttgg acgagcaata tcaggttgcc tacaaaatcc
    11881 tcgatgaaaa ccgcgacaag atggaaacga tgtgcaaagc cctgatggaa tgggaaacca
    11941 tcgaccgcga tcaggtactg gaaatcatgg cgggcaaaca acccagtccg cccaaggatt
    12001 acagccacaa cctgcgcgag aatgcggacg cggcggaaga taacgcgccg cacgctccga
    12061 ctcgggaaga aaccgaagca cctgccccgg cagacaccgc ttcgacagag tccgagcagc
    12121 agcctgaaaa caaggcttaa cttcccgaac aaacggcagc ccgcaagctg ccgttttccc
    12181 atcctgtttt cagacggcat tttccgctca atgccgtctg aaacgccaaa gccgtatcgg
    12241 tttttgcgga tctgcgatat ggcggtttaa attgaaatca gacgaggcaa ggctgtaccg
    12301 gtttaaaatt aaaccgctat aataccgcct ttcgaataca cgcccgcatt ggtgatgcct
    12361 gtgaaaaaag ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat gttcgagctg
    12421 gtcgataagg ttgaagacta tccgcacttt ctgccgtggt acagcaagac cgaggtcatc
    12481 gggcgtagcg gcaacgaact gaaggcgcgg ctgtttatgg actatatgca cgttcgccaa
    12541 tcattcgcca cgcacaaccg caacattccg ggcagggaaa tccgtatgga actgctcgag
    12601 ggtccgttca aaaccttacg tggaacgtgg aaatttatcg atttgggcga cgatatgtgc
    12661 aaaatcgaat tcaatttgga atacgatttt tccaatgccg ttttgtccgc cttaatttcc
    12721 cccgtcttca accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga ggcagaccgc
    12781 cgttatgctt gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt tgaaaaccat
    12841 gcagcttgcc gagggaacaa ccgtccgcgc cgccgcactg caaagcggtt tggacggcat
    12901 atttgaagat ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag ccgtcaaaga
    12961 cgacacgccc ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt tgatcgaccc
    13021 caaagaagcg cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc gtctgaagcc
    13081 ttcagacagc attcgacaga aacacggaaa atatcatgtc aaacaaaatc aaaatgcttg
    13141 tcggattggg caaccccggc aaagaatacg aacaaacccg acacaatgca ggcttttggt
    13201 tcctcgacga actggcttgg aagtggaagg cttcatttaa agaagaaaaa aaattcttcg
    13261 gcgaagtcgc ccgtgccgcc ctgcccgacg gcgatgtctg gctgctcaag ccgaccacgt
    13321 tcatgaaccg ttccggacag gcggttgccg cccttgcgca gttctacaaa atcaaacccg
    13381 aagaaatcct cgtcgtccac gacgaactcg acattccctg cggacggatt aaattcaaac
    13441 tcggcggcgg caacggcgga cacaacggct tgaaagacat tcaggcaaaa ctcggcacgg
    13501 cagactatta ccgcctgcgc ctcggtatcg accacccagg cgaccgcaac ctcgtcgtcg
    13561 gctatgtcct gaacaaaccc agtacggaac accgccgaca gattgacgat gccgtcgcca
    13621 aatccctgca agccataccc gacatccttg ccggcaaatg ggaagaagca acccgcttcc
    13681 tgcacagcaa atgacccgat gccgtctgaa gccctttcag acggcatgtt cccgatttcc
    13741 atatccgaac agtcatgacc gaactcaagc agcttatcca aaccgaatcc atccccgtca
    13801 tcgaagaaac cctcgatttc ctgctgtacg aatgcagcat cgacgacgca ccatccgccg
    13861 aagaagtggc acaatggcgc gacatacttg ccgcacgcgg cggcaaattc ctgcgcctgt
    13921 ccaaaatctg ccaaacgtgg ctggacgagg aggcggcatg aagctgccgc gcaaccgctt
    13981 cagcctgctt tccgcattgt ggtttgccgg cggcatctat tcgctgctct tcaaagctgc
    14041 cgacaccgcg ccgccgccgt ttccgcattt cgacaaagca gcacaccttg ccctgttttt
    14101 cgcacaaatc tggcttttga ccaaagcatt caaaaccgga aaacttccca tcccctaccg
    14161 cagcctgatg gtctttgccc tctgtttcgc cctcttcagc gaatgcgcgc aggcatggtt
    14221 taccgcaacg agaaccggca gtttgggcga tgttcttgcc gatatggcag gtacggttct
    14281 cgcactcttt gccgcccgcg ccgccgaccg cccggactga atcggtttat ttcccccaaa
    14341 caggatgcac ctcttcccga tacatcctat ttttcccctt acatatcaat cccatctcat
    14401 aaacaaaacc gacaaatcgt ttacaatata tttacactac atcagattac aaatatactc
    14461 gaaccagttg cagaatcggc ggttccacaa accgtttcgg attgcacgac acaaaagaaa
    14521 aaccgatttt attgccttaa aggagtattc atgaacctgc atgcaaagga caaaacccag
    14581 catcccgaaa acgtcgagct gctcagtgcg cagaagccga ttaccgactt taagggcctg
    14641 ctgaccacca ttatttccgc cgtcgtctgt ttcggcattt accacatcct gccttacagc
    14701 cccgatgcca ataaaggtat cgcgctgctg attttcgttg ccgcactttg gtttaccgaa
    14761 gccgtccaca ttaccgtaac cgcactgatg gtgccgattc tcgccgtcgt actcggtttc
    14821 cccgacatgg acatcaaaaa ggcgatggct gatttttcca acccgattat ctacattttt
    14881 ttcggcggct tcgcgcttgc caccgccctg catatgcagc ggctggaccg taaaatcgcc
    14941 gtcagcctgt tgcgcctgtc gcgcggcaat atgaaagtgg cggttttgat gttgttcctc
    15001 gttaccgcct ttctgtccat gtggatcagc aacaccgcca ccgccgcgat gatgctgcct
    15061 ctagcaatgg gtatgctgag ccacctcgac caggaaaaag aacacaaaac ctacgtcttc
    15121 ctcctgctcg gcatcgccta ttgcgccagc atcggcggct tgggcacgct cgtcggctcg
    15181 ccgcccaacc tgattgccgc caaagcccta aatctggact tcgtcggctg gatgaagctc
    15241 ggcctgccga tgatgctgtt gattctgccc ttgatgctgc tctccctgta cgtcatcctc
    15301 aaacctaatt tgaacgaacg cgtggaaatc aaagccgaat ccatcccttg gacgctgcac
    15361 cgcgtgatcg cgctgttgat tttccttgcc acagccgccg cgtggatatt cagctccaaa
    15421 atcaaaaccg ccttcggcat ttccaatccc gacaccgtta tcgccctgag tgccgccgtc
    15481 gccgtcgttg tatttggtgt ggcgcaatgg aaagaagtcg cccgcaatac cgactggggc
    15541 gtgctgatgc tcttcggcgg cggcatcagc ctgagcacgc tgttgaaaac atccggcgcg
    15601 tccgaagcct tgggacagca ggttgccgcc accttttccg gcgcgcccgc atttttgatg
    15661 atactcatcg tcgccgcctt catcatcttc ctgaccgagt tcaccagcaa caccgcctcc
    15721 gccgcattgc ttgtaccgat tttctccggc atcgccatgc agatggggct gccggaacaa
    15781 gtcttggtat tcgtcatcgg cattggtgca tcttgtgcct tcatgttgcc ggttgccaca
    15841 ccgcccaacg cgattgtgtt cggtacaggc ttgattaagc aacgcgaaat gatgaatgtc
    15901 ggcttattgc tgaacatcct ctgcgtggta ttggttgcgc tgtgggctta tgctgtactg
    15961 gtgtaaacca tcgacctaaa caacaaggcc gtctgaaaga atatttttca gacggccttg
    16021 aagtttgtcg ttcagacaca atttaacgaa tgatttaaaa ccggattcta acgaaaggaa
    16081 acccatgatt atcctgcaca ccaacaaagg cgacatcaaa atcgaactcg atttcgacaa
    16141 agcccccgtt actgctaaaa acttcgagca atacgttaaa gacggcttct acgacggcgt
    16201 aatcttccac cgcgtcatca aaggcttcat gattcaaggc ggcggcatgg atgaaaacat
    16261 gaacgaaaaa gaaacccgcg atccgattca aaacgaagcg tccaacggtt tgcctaacga
    16321 aaaatacacc atcgccatgg cgcgtacttc cgatcctcac tctgccagcg cacaattctt
    16381 catcaacact gccgacaacg ctttcctgaa cttccgttct aaagagttgt acggcaaaac
    16441 cgtcgtccaa gactggggct atgccgtatt cggtaaagtc gttgacggtt ttgacgttgt
    16501 cgatgccatc gaaggcgttt ctaccaaacg tcatggttac cacgacgacg taccgagcga
    16561 acctgtcgtc atcattaaag ccgaagcggt ataaaccgac aatccgaaag cagcctgcac
    16621 aaaggctgct tttatttcag acggcatgtt atttgatgta accgtgctat gccgtctgaa
    16681 aaccggacgc aagccttcag acggcatacc gctatgttaa aatatgcccg tttttcctaa
    16741 acggacacaa aggaaacctt atggcaagca tcgcccgcga catcttcaaa gcctacgaca
    16801 tccggggtat tgtcggcaaa accctgaccg acgaagccgc ctaccttatc ggcaaagcca
    16861 tcgccgccaa agccgccgaa aaaggcatta cccgcatcgc gctcggacgc gacggacgct
    16921 tgagcggtcc cgaactgatg gaacacatcc ggcgcggctt taccgacagc ggcatcaatg
    16981 tcctcaatgt cggtatggtt gccactccta tgctctactt cgccgccgtc aacgaatgcg
    17041 gcggcagcgg cgtgatgatt accggcagcc acaatccgcc cgattacaac ggctttaaaa
    17101 tgatgctcgg cggcgacacg cttgccggcg aagccatcca agaacttttg tccatcattg
    17161 aaaaagacgg ttttgctgcc gccggcaaac aaggcagcgt caccgaaaaa gacatctccg
    17221 gcgaatacct caaacacatt accggacaca tcaggctcaa acgcccgatg aacatcgcca
    17281 ttgacgcggg caacggcgtg ggcggcgcgt ttgccggcaa actctacaaa ggcttgggca
    17341 acgaagtaac cgagcttttc tgcgacgtgg acggcacttt ccccaaccac catcccgacc
    17401 catccaaacc gaaaaacctg caagatttga ttgccgcgct gaaaaacggt gatgccgaaa
    17461 tcggcttggc gtttgacggc gatgccgacc gcttgggtgt ggtcaccaaa gacggcaaca
    17521 ttatttatcc cgaccgccaa ctgatgcttt tcgcgcaaga cgtgttgagc cgcaatccta
    17581 aagccaaagt gattttcgac gtcaaatcca cccgtctgct tgcgccttgg attaaagaac
    17641 acggcggcga acctgtgatg gaaaaaaccg gccacagctt tatcaaatcc gccatgaaaa
    17701 aaaccggcgc gccggttgcc ggcgaaatga gcggacacat cttcttcaaa gaacgctggt
    17761 tcggcttcga cgacggtctg tacgccggcg cacgcctctt ggaaatcctg tctgcctccg
    17821 ataatccgtc cgaagtgtta aacaacctgc cgcaaagcat ttccacgccc gaactcaaca
    17881 tcgccctgcc cgaaggcagc aacggccatc aggttatcga cgaactcgcc gccaaagccg
    17941 aatttgaagg cgcaaccgaa atcatcacca tcgacggtct gcgcgttgaa tttcccgacg
    18001 gcttcggtct gatgcgtgct tccaatacca cgccgattct ggtgttgcgt tttgaggcgg
    18061 atacgcaaga agccatcgag cggattcaaa accaattcaa agccgtcatc gaaagcaatc
    18121 cgaatctaat ctggcctctg taaacacagg aaaaatgctt aatcaaatct tggtagattg
    18181 gatttggctt aaaaatctcc gcgtccgttc gtgtttgggg tggtcgaaca actctttcgg
    18241 gctgccctgc tctacgataa cgccgccgtc catcacgacg acggtcgtgg caacttccag
    18301 cgcgaacttg atttcgtggg taacgacgac catcgtccaa ccttcccgcg ccaattcctt
    18361 catggcgttc aacacgtctt gcaccaactc ggggtcaagc gcggaagtgg gttcgtcaaa
    18421 caacatcagc tcgggctgaa tcgccaatgc tcgggcaatg ccgacgcgct gctgctgacc
    18481 gccggaaagc tggtagggat acaaatccac tttgtcgccc aagccgactt tttccagcag
    18541 tttcagagcc tcttcgcgcg cttgggcggc aggcttgccc tgtacggcaa ccggtccttc
    18601 catcacgttt tccaaggcgg ttttgtgcgg aaagaggttg tattgttgaa acaccatgcc
    18661 tgatttgcgg cgcagtgcca aaatatcgtg tttgcttggt tttttagaaa aatcgatttt
    18721 cagcggtcgc tcgttgtcga actcgatttg tccgtcttcg ggcatttcca acgcgtttag
    18781 gcagcgcaga aacgtcgttt tgcctgagcc ggaaggcccg aggatgacga ccacctgccc
    18841 tttgcacaca tccaaatcga tgccgcgcaa aatggtattt tcgccgaagg tcttatggat
    18901 attgcggatt ttaatcatga caactcctca tttggcgaca tagcggtcga aacgtttttc
    18961 caaacgcgcc tgaatcagaa acagcacttt gcagaaacac cagtaaacca aagcggcttc
    19021 gatatagacg ggcaaaaagt cataagtgcg gtttgccgtc tcttgggcga cacgaaaaag
    19081 ctctgttacc gttaccaccg ccgcgagcga ggtgtttttg aacaagccga taaattcgtt
    19141 gctcaaaggc ggcacggcaa cgcgaaatgc ctgcggcgcg acgatgcggc ggaacgtctg
    19201 catataggtc atgccgatgg agaaacctgc ttcccattgg cctttcggta cggacaaaat
    19261 tgccgcgcgt atggtttcgg aagcatatgc gccgacattg agcgaaaagc cgatgatggc
    19321 ggcaggaatc gggtcgatat agatgccgac ggaaggcagc ccgtaaaaca caatcacaag
    19381 ctgaaccaac agcggcgtac cgcgaatgac ggaaatataa aattccacca atttcagcag
    19441 gattttccgc acgatgccgc cggcgggcat aatccgcacc aaagccacgg ctaccgcaat
    19501 catcataccg ataacgaaag aagctgccgc caaaggcaga gagaccgcga agccggcttt
    19561 gaccataggc aaaaacgcgc tgacaatcat atcggcgcgt gtttccgtca taaacggcag
    19621 cgaagcaagg aaattattga acactgatgt cttttccgaa gaattgttcg cccagttttt
    19681 tcagcgtacc gtcggctttc agctcgttga ttgccgtact gaatttcgcc acggcttcgt
    19741 cattgccctt gttgacaatc aggccggaac cgactttttc atcggcaggt gcggaccaaa
    19801 cgattttcac gcccgcattc gggtttttct tcagatagtc caaaaccgcc aattcgtcgt
    19861 tcagggttgc atcggcacgt ttttgttcaa tcagggtcag cgattgcgcc aaaccgtcaa
    19921 cagccaccaa atctgcgcct gcagctttgg ctttttcgcc gtagttgctg gtcagggatt
    19981 gtgcggtttt cacgcctttg atgtcgtcga tagatttgat gttgctgtcg ttacgggcaa
    20041 ccaatacggc accgctccag ctgtaaggat cggatttgtc gaatgtcgct tggcgttcgg
    20101 ggctggtcag accgacttgg tttgccacca cgtcgaaacg ccccgccttc aaacccgcca
    20161 tcatcgaatc ccattgcgtt tctttaaact cgactttcac gcccagtttt tccgccacgg
    20221 cgcgggttac ttccacatcg taaccggtca gtttgccgtc cttgtcgtgg taggtaaacg
    20281 gtgcgtaagt gccttccgtg ccgacggtaa ccgtgccttt attgttgatg cgctcgatta
    20341 aagaaccgga aactgccgat tgtgcaggcg cggaagatgc tccgctgccg ccttccgaac
    20401 cgccgcaggc cgccaaaacc aatgcggcaa taccgccgag tacgaatttt ttcaacatca
    20461 ttctctcctg taaaaagtgt gcaagtgcgg cattctaagt gaaattgcgg caaacacaaa
    20521 gaatcctttt tttatatggt tattccaaat cggaatggaa aagatatgcc gaagccgccg
    20581 gaagcggaaa taaagggggc gggaaaggcg gcgggcggca cgaaaccgaa aagcggcatc
    20641 aggtatgcaa acggcggatt cacgcacgcc gttccggtaa aaatccgcct gccgattcgg
    20701 tgctataatc catccgaaac agatgacacc aaaaaggaac atctcatgaa cacgccccaa
    20761 tccgccatca ttcccgacca cgcccaagcc ggcatcttta tcgaagccga cttcgccgcc
    20821 aaccgcctca acgatattaa agccgcctgc cgcgcttcgc tcgacgcgtt aactgccttg
    20881 aaagcccgct ttccggacga tattttgggt ctgaccatcg ccttcggcag caaagcctgg
    20941 gcaacattcg gacatacgga cgaaggcagt gaaatcaaac ccttctccga aatgggcaac
    21001 gggcttgcgc cgtccacgca gcacgatatg tccatccaca tccaatcctt ccgccaaaac
    21061 gccgcctacg cgcttgccca atccgttttg ggcgcgttcg gcgacagcat atgcgtcgcg
    21121 tccgaagaac acggtttgcg cctgtatcag gatcgcggac tggacggttt cgtcgacggc
    21181 acggaaaacc cgcagggcga tgaaaatgtc cgcgaagtcg ccatcatccc cgaaggaaaa
    21241 cccgatgcgg gcggcagcta tgtcctgctg caaaaatacc tgcacgattt gaaaaaatgg
    21301 gatgccgtcc ccgtcgccga acaggaggcc tcggtcggac gcggcaagga aaccgacgac
    21361 gaattcggcc gcgatgtccg cctgcccgat tcgcacctcg gccgcgtcaa cctgaaagaa
    21421 aacggagtcg gcctgaaaat cgtccgccgc agcctgccct tcggcaaaat cagcggcgaa
    21481 cacggtttga tgtttaccgc atactgccgc actctgcaca atatcgaagc gcaattattg
    21541 agtatgttcg gcgatacaga cggcaaaacc gacctgctgc tccgacacct ctccgccgcc
    21601 gtttcgggcg gatactacta cgcgccgtct gtcgaacgcc tgcaaaacct ctaagccgga
    21661 cgatgccgtc tgaaaccatt tttcagacgg cattttcccg ttttatagtg gattaacaaa
    21721 aaccagtacg gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
    21781 accaagtgaa tcggttccgt actatttgca ctgtctgcgg cttcgtcgcc ttgtcctgat
    21841 ttttgttaat ccactatacc gtccaaccgt tttccgagcg cgcactatgt ctgcacccat
    21901 ccaagcattc gactcgctaa ccgtccccat tgccggcacc aacctgattg aggcctctgc
    21961 cggcaccggc aaaacctacg gcattgccgc cctgtttacg cgcctgatcg tattagaaca
    22021 aaaaaacgtc gagcgcgtat tggtcgttac cttcaccaaa gccgccaccg ccgagttgaa
    22081 aacacgcctg cgcgcacgtt tggacgatgt gttgcaggtt ttagaaagta aagaaattgc
    22141 caaacttgga gacgacacgc tttcagacgg catcgccgcc tattgtgcgg aacaccacaa
    22201 aggcgacacc ttcctgcccg cactcttaaa acaggctttg caaaaagagg gccggacgcg
    22261 tctgattgtc cgcctcaagg ccgccatcgg gcaattcgac aacgccgcca tctataccat
    22321 ccacggcttc tgccaacgta tcctgcgcga ctacgccttc ttgtgccaag caccgttcga
    22381 tgtcgaactg accgaagaag acggcgaccg cctgcttatc ccggcgcaag atttttggcg
    22441 ggaacgcgtc agcaatgatc cggtgcttgc cgcattggcg tttaaacgca aagctgtgcc
    22501 gcaaaccgtc cttgcccaaa tccgcgccta cctgtcccgc ccgtacctga atttccgccg
    22561 tccgcaggca gatttgaaac aggcgcagca caacgccgaa acctcatggc aaaccgtctg
    22621 ccgcctgctg cccgagctgg aagccggctt ttggcgcatc catcccgacc tcaacggcaa
    22681 cagttaccgc aaaaacagct tcggcaacct ttttaaagaa ctggcacaaa aatccgccgc
    22741 cggacaactg ccctttctgg acaaagacac gcacgacaga ctcttgaaac ttgcatccga
    22801 caaactcgaa gccggactga aaaaaggcaa aacgcccgat gcggcagtat ttgccgaatt
    22861 gcagaaactg gcagacttcg ggcgcgattt gaacgcactc gaagaagcgg aagaagcaac
    22921 aatgatccgg ctgcaactgg atttaatcga atacctcaac cgcagccttg ccgagatgaa
    22981 aaaatcgcgc cgcgaacgcg gtttcgacga cctgctgctc gatgtccaca ccgcgctgac
    23041 cgacaatccg cacgccgaaa ccctcgcccg cgccgttgcc gaaaactggg aaaccgcgct
    23101 gatcgacgag ttccaagaca ccgacccgct gcaatacgaa atcttccaaa aaatcttcat
    23161 cgcccaaaac cgaccgctgt tcctcgtcgg cgatcccaaa caggcgattt acagctttcg
    23221 cggagcggac atttacgcct accttcaggc ggcggaagat gcgcggcacc gctacacgct
    23281 cgccaccaac taccgcagcc acgccgcgct tatcggcagc ataggcgcgc tgttccgcct
    23341 caaagaacgc ccgttcgttt tggaaaacat cggctattcg gaagtcggtg cggcgcgtgc
    23401 cgaaagcagg ttgtcgccca aacgtcctgc cgtacaggtt cgttggctgc acgaaaacga
    23461 caatgaaaaa gccaacaaag acgttttgcg ccgccgtgcc gccgactatt gcgccgacga
    23521 aatcgcccac gcgctcaacg aagccgccgg aggccgtctg aatttcaaag gctgcccgtt
    23581 gcagtcgggc gatattgccg tgctggtacg cacgcacaac gaggcggtga tggtttccgc
    23641 cgccctgaaa aaacggcagg tgcaaagcgt cctgctttcg cgcgaatccg tgttcgcctc
    23701 gcccgaagcc gccgccctgt ccgcactcat cggcttctgg ctcgaaccgc gccgcgccgg
    23761 aacgctgcgc tttgtcctga caagcggcat attcggttat gacgcgcagc aattgcacga
    23821 cttcaaccaa aacgaaagcg agattttgca ttgggcggaa tctgcccgaa ccgcgctcga
    23881 caactggaat caatacggca ttttcgccgc catgcagcaa ttttcccaaa cacacggcat
    23941 cgaaacgcgc ctcttaagcc ggaacaacgg gcgcagcctg accaattatt tccaactgct
    24001 cgaactgctt gccgccgaag acgcccaaaa ccgcaacccc gccgcgctgc acaaatggtt
    24061 gcgcgaccaa atcagcctgg ccgaaaacaa cagcaacgac aaccgcgcca tccgtctgga
    24121 aagcgacgaa gatttggtca aaatcgttac catgcacgcc tcgaaaggtt tgcagtatcc
    24181 gctggtgtac tgcccgtttg cgtgggacgc gcaagatacc ggaccgtccg actggcaaat
    24241 cctccaccaa agcgccaacc gaaccgaact gttggcaaag gcgcaactgt cggaagacga
    24301 acagaagcaa tacgccgacg aagaaatggc ggaacgcctg cgcctgcttt atgtcgcgct
    24361 gacgcgtgca gaggaacagc tcaacatcta cgccgcctat tcttccgata ccgccgacaa
    24421 ccccctcgcc tacctgatcg aaggcttgcc gcaagacagc cgcgaaaccg tccgccgtgc
    24481 ctatgcgtgt gaaaaagacg gcatcgcgat gctcaaacgc aactggcggc gtttggcgga
    24541 caacgccccg tccggcacgg atttcgcctt cacagaagat gcgccgccgc ctgccgtata
    24601 tcgcggcaac gccggtcaaa ccgccgaatt tgccgcaaac agcattcccg aacgcggatt
    24661 ccgatttgtc cgccacacca gcttcaccgc cttaagccgc catacccaaa cgcccgacgg
    24721 cggcgaagaa gatgcctgcc cgtccttgga tgccgccgaa acctcggtag cggcgatgcc
    24781 gtctgaaacg ccgacggctt cagacggcat atcgatacac gactttccga aaggcacgca
    24841 ggcggggctg tgcctgcacg aaattcttga agatttcaaa ttcggacaag cagccgccgg
    24901 acaggaaacc ctcatcaccg acaagctgaa aaaatacgga tttgaagaaa tatggctgcc
    24961 cgccgttgcc gaaatggcgg aagcctgcca gaaaacgccg ctgacggggg catactgcct
    25021 gtccgacatc ccgcccgagt gccgctgtcc cgaaatgggc tttaccctcc acaccgaaga
    25081 cttcggcctc aaacggctgc gcgactggtt tgcccgcgac gacatcaggc tgcccgaagt
    25141 ctgccgtgcc gccgccgaaa cgctcgactt ccacaccgtc aacggctttt taaacggctt
    25201 tatcgatatg gtctgccaag accccgacgg caatatctgc gtcatcgact acaaatcaaa
    25261 ccatctcggc gcggacgcat ccgcctacac gcggcaggcg atggacgaag ccgtcgcgca
    25321 ccaccactat taccttcagg cgctgattta cgccgttgcc gccgcacgct acttcaaact
    25381 gcgcggacaa ccgcccgccg ccgtttccgt ccgctacctg tttttgcgcg gattggatgg
    25441 caaaggcggc ggcgtttggc gttgggacat agatgccgcc gctttggaac agataaaata
    25501 acttcccgtc ccttaaaacg acaaccgcag gagaacccta tgaatatcaa acacctgatt
    25561 accgccgcac tcattgcctc agccgccttt gccgcgcagg cagccccgca aaaaccccta
    25621 tccgccgccc aaaccgcgca acatccggcc gtttggatcg atgtccgttc cgaacaggaa
    25681 tttagcgaag ggcatttgca caacgcggtc aacatccccg tcgaccaaat cgtccgccgc
    25741 atacacgaag ccgcgcccga caaagacacg ccggtcaacc tctactgccg cagcggacgg
    25801 cgtgccgaag ccgcccttca agagctgaaa aaagcaggtt atacaaatgt tgccaatcac
    25861 ggcggttatg aagacctgct caaaaaaggg atgaaatgac gcaatgccgt ctgaaaggac
    25921 aggctttcag acggcatacg gcgttccggc acgcggcgca acatcgggcg cggcgcgtgc
    25981 cgccccgtcc gaagcctgat aaacaaaccg ataaggaaat acgatgaaac acatactccc
    26041 cctgattgcc gcatccgcac tctgcatttc aaccgcttcg gcacatcctg ccggcgaacc
    26101 gtccacccaa aacgaaaccg ctatgaccac gcataccctc acctcaaaat acagttttga
    26161 cgaaaccgtc agccgccttg aaaccgccat aaaaagcaaa gggatggaca tttttgccgt
    26221 catcgaccat caggaagccg cccaccgaaa cggcttaacg atgcagccgg caaaagtcat
    26281 cgtcttcggc acgcccaaag ccggcacgcc gctgatggtc aaagaccccg ccttcgccct
    26341 gcaactgccc ctgcgcgtcc tcgttaccga aacagacggc aaagtacgcg ccgcctatac
    26401 cgatacgcgc gccctcatcg ccggcagccg catcggtttt gacgaagtgg caaacacttt
    26461 ggcaaacgcc gaaaaactga tacaaaaaac cgtaggcgaa taagcccgtc gccgacatat
    26521 ggcggatgga aatcaaacac acacgcccct cattcccacg aagtcgggaa tccaagcggt
    26581 tgggttaccg tgattttcaa tcattgccga aacggacgac tccagattcc cacccttgcg
    26641 gggatgacgg gcgtgtgtgt ttttgccata tcacaggtaa ctttgccaca aatcttatct
    26701 gtcgccggta tgccttcttg ttttcaatcc gccacaaacc aaatgccgtc tgaaccttca
    26761 gacggcattt cctttcaatc cctatcttcc cgacaaatat cgatggcttc ctgcaaactc
    26821 gaaacgccgt agattttcag gttcggaaac tctttggcgt tgcgcggcat attggctttt
    26881 gggacgatgg cgcgtttaaa gccgagcttt tccgcctctt tgagccgctc ttgtccgcgt
    26941 gcgacggggc ggacttcgcc gcttaagccg atttcgccga aaaccacggt tttttcgggc
    27001 atggggcggt tgcggaagct ggagagcatg gcaaggatga cggcaaggtc ggcggcgggt
    27061 tcgccaattt tgacgccgcc gacggcattg agaaacacgt cttggtcgaa acaggcaatg
    27121 ccgccgtggc ggttgaggac ggcgagcagc atcgcaaggc ggttttgttc cagtccgacg
    27181 gtaaggcgtt tgggcgtgaa gccgtgcgcg tcatcgacca atgcctgaat ttcaaccaaa
    27241 agcgggcggc tgccttcctg cgtaaccaaa acgcacgaac cgggcgtgtc gtcgcggtag
    27301 ctggcaagga agatggcgga cgggttggac acacctttca aaccgttttc cgtcatcgcg
    27361 aacacgccca gttcgtttgc cgcgccgaag cggtttttga tggcgcgtat catgcggtag
    27421 ttggaatgtt ggtcgccctc gaaatacagc acggtatcaa ccatgtgttc cagcacgcgc
    27481 gggccggcaa tcgcgccgtc tttggtcacg tgtccgacca gtatcatggc gatgcccatc
    27541 tgtttcgcca tgcgcgtcag ttgggctgca cactcgcgca cctgcgacac ggagccgggg
    27601 gcggaagtga tttggtcgga atacatggtt tggatggaat cgataaccac gacttcgggc
    27661 tgatgctgtt tcaaggccgt ctgaatggct tccatgcgga tttcggcaag caggttcacg
    27721 ccttcggtgg gcagttccaa acgttgcgcg cgcaaggcga cctgttgggc ggattcctcg
    27781 ccggaaacgt acagcacttt gcggctttgc gccattttgg cgatggtttg caacagcagc
    27841 gtggatttgc cgatgccggg atcgccgccg agcaggatga ccgcgccatc gaccaaaccg
    27901 ccacccaata cgcggtcgag ttcgcccata ccggtcggat tgcgcggcac ttcggtggcg
    27961 gtcacggcgg agagggactg gacggtcgag gtatccgccg cccaagattg gaaacgggcg
    28021 tttttcggct cgggcgcggc taagctttcc tgaagtgtgt tccactcgcc gcaatgcggg
    28081 catttgccct gccatttcgg ggaagtgccg ccgcattcgg tgcattggta aagggtttta
    28141 agcgtttttg ccatcggttt tccctgcatc tgaaaacaaa aatgccgtct gaaaataggg
    28201 acggttggga ttgcgccaat gcggcaacgc cgccgtcatg tactatccgt acacggcgga
    28261 tattgccgca ttgccttatt tttaccccaa ccggccatct ctcggcttca gacggcatat
    28321 cgggcggttt cctgccggct tacccaccgt gataatgccg cgacaattcg tgtacggtat
    28381 cgactaagat tttggcgtgt tcgggatcgg cgtgctggtt gatgccgtgt ccgaggttga
    28441 agacatgacc gctgccttgt ccgtagtcgg ccaggatacg tgcggcttcg gtgcggatgg
    28501 attcgggcgt accgaagagg gcaaacgggt cgaagttgcc ttgcagggcg acttgcttgc
    28561 cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc caagcccaat gcgtctgcgc
    28621 cgatttctgc catgctttcc agccacagcc cgccgccttt ggcaaatacg ataacaggca
    28681 cgcggcgacc ttcgctttcg cgtttcagtc cggcaacgat ttggcggatg tatttgaggc
    28741 tgaattcttt aaacgccgcg tcgctcaaca cgccgcccca agtgtcgaaa atctgcacag
    28801 cctgtgcgcc cgcgtcgatt tgggcgttga ggtaggcggt aacggcttgg gcgttggtgt
    28861 cgaggatttt gtgcagcaaa tcagggcgcg aatacatcat ggttttgatg gtgcggaatt
    28921 ctttgctgcc gccgccttcg accatgtagc aggcgagcgt aaacgggctg ccggagaagc
    28981 cgatgagcgg tacgcggccg tccaatgctt tgcggatgga agttaccgcg tcgaaaacgt
    29041 attgcagttt ttccatgtcg ggaacgtgca gcttggcgat atcggcttcg tgttgcaggg
    29101 cgcgtttgaa tttcgggcct tcgccttcgg caaaatacag tcccaagccc attgcgtcgg
    29161 ggacggtcag gatgtcggaa aacaaaatcg ccgcgtccaa atcgaagcgt tccaaaggtt
    29221 ggatggtaac ttcggtcgcc aattcggtgt ttttgcacaa atcgaggaag ctgcccgctt
    29281 tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc ctgacgcatc atccaaatcg
    29341 gcgtgtattc gacgggctgt ttgagcaggg cgcggaggaa agtatcgttt tttaaaagag
    29401 tcatgcgggt tttctgtcag tctgtttgtc aagtgaaatg gttttcagac ggcatttcaa
    29461 cgatgccgtc tgaaggtttc aacaggtttc ggcggaaggg cggttttcct cggcaacgct
    29521 tgccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccggttt cgtcaaaaac
    29581 ctttgccaga accaaacgcg cggaaatact cggctttaat gcaatgctcg cttcaaggta
    29641 gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat acatcagcag
    29701 aagcgcgtta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg ctttctgctg
    29761 atcgcgttcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagtt cgggtcggcg
    29821 gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc ccaaacgttc
    29881 gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg gaatccgctt
    29941 caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc ggtatgccca
    30001 attttgatac tgtttcgttt cggattcacc caacgcacca gccttggaaa gtttttcggt
    30061 ttttgccaac acctcaagcg cgtctcctct ctcgaaggca tagcgcaatt gcaggcgcac
    30121 aagccgtgtc agtccggcat tcattttggc tgccgcatga agattggctt ccaccgtttc
    30181 gtagtcccgg cgattcagtg ccgattccgc cagcaggagg tagcgtgaaa gctgctgttt
    30241 ttcaggcagc tttgcaattt ctgcaaggta ccggtcgcgt aattcggtat ttcccatctg
    30301 tcctgcggcg tgcgcgccca gcatcaatgc caaagtccgg ttgtctccgg cctctttgtt
    30361 gcccaacacg cgcgaggctt cgagttccgc cttttcaaaa cgcccttcaa aatacgccaa
    30421 acccgctttg ttcaagtaaa gcgcggcctt gcgtcctttt cgtgccaaac tgaaacgctg
    30481 catcttttcg ggaatattca aaatgccgat gacgaattta aacaggaaat accacaccac
    30541 gacggcaatc agcagcccca gtacaaaggc gtgcaggttg attctgagca tggtctgtcc
    30601 gagtacgata tacacgtcgc cggtgtaaat gcccaaagcc aatgccagcc cgacggcggc
    30661 ggcaaacagg atgacgatcc agactaccgt tttcatgcgc gttctccttt agcggacggc
    30721 gaacgccctg ccttttccgg tacggtctgt ttttttgcag gctgttccgg tttaagttcc
    30781 gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccga ttcggaagct
    30841 gtcttttcgt ttgccggttc ggaagccgcc tgttctgctt cttgattttc cgccgccgtc
    30901 atgcgcgtac cgtcgcgata ggcgcggacg gcatttaaac tggccttcaa accgtcatcc
    30961 gccgtcatgc gcacatccaa cgccttcaat tccgccaatt ccttcaacca cgactgcgtg
    31021 gcgggggatt tggcatcaaa atactgtctg acggcggttt cggcattgtt caaatcgctt
    31081 tgatagactt cgctgttgcg ctgcattaat gcagtccgcg catccaaaag gcggaggcgc
    31141 agattttcgc gtacaaaata tgcctgttcg ggagaaatca gcatggcatc gttgttttcc
    31201 aaacggcgga tttcaaccag ccctttcaat gtgccgaggg atttttccca tacgttccac
    31261 catgaagcgg aaacggcttc gttgcgggcc tgtacgcccg gtttcagcac gccgtcgagc
    31321 atcaacggca gtccggatac ggcggtttcc agcctgtcga tgcgcagtgc cgtgccggaa
    31381 atatcgacat aaggacggtt tttcagttcc gccaagtcgc tgctgattgc ctgcttgagc
    31441 ggcaggagct ccgcctgatc gaaacgggac aggcggctgt cgatatgctc caacacgccg
    31501 actgccgttt ggatattgcc ggtcaacacc agctgttgcg ctgccagatt gagtatggtc
    31561 tcggtttcgt ccaccagcca atcggcgcgt cctttggtca actcgcgata ggatttttgc
    31621 gtcatcagaa tctgttcgcc gttggctttg acaccgctgt ccaaacggtc gagttcggac
    31681 tgtatggcac tttggcggtt gaggttgtct ttgagcaggg cggcgttttc cgactcgccc
    31741 aaggcggctt tgtcgatttt ttggttgaat gccagctctt ggtttttcaa gacattctgt
    31801 ccttggacaa acaaaaaacc acttgcgccc aaacccaatg ccgccaatac caaggcgcag
    31861 gttgccaaag cgttgctgcc ggactgtttg acgataaaag gagcggcagg aatgtgtact
    31921 tctgtttcgt tttctttgcg tggggaagag gtttcagacg gcatttcttt tgatgcctgt
    31981 atctcgcgta cgggttcgga tgatttgttt tcaggttcgc ccaccgtttt gctccttatt
    32041 aatttgaggt tccgggaaag accatgccgt ctgaaacgga aatggaagaa tgggaaagcg
    32101 cggcttccag cgtagggacg gtttcgaccg aacacacgcc ttcgcgcttc aatgcctccg
    32161 caatgcgcgg atgatgggta aagtatagca aggatttgaa gaatcgggaa aattgcggcg
    32221 gaagctgccc gaacagcaag cgcaccagct cggtcgacgt aatataggcg gcggcaatat
    32281 tttcggtttg gaaattttga aagttcaaag gtttatgccg tctgaaatag acttctgcca
    32341 cctccgtccg aaaacctttc tcctgcaagg cattcatcaa aaaatcccgc ccgccgtgtc
    32401 cgcgcacaaa caatacgcgc gcaccttcgg gcagactgtt ccaaaccggc aggcgcaaaa
    32461 ccgcctcgct gtcgttgccg tcatcaggcg cgatgaccgt tctgaccaaa cagcgttcca
    32521 atgcgcggcg gctgccctgc cctacggcaa tctgcgcctt tatgccgtct gaaaggttaa
    32581 ggtacgggac ggcggtttca acggcggtcg gactgaccca aaacacggca tccgcacggg
    32641 catattgttc ggacaaaagt tccagtccgg cagcatctgc ttcgatttca atcgggctca
    32701 acacttccgc gcgccaaccg gcattcaggc aagtttcgac atcttctgcg gccctgcccg
    32761 acggacggac aatcagcatg acgggtttcg ctgtttccat cgccttatcc gccccgatta
    32821 ctaggaaaaa tcgtcatcgt tgaaattcgc ctgcgcggtt ttcggcgtac agccgacccg
    32881 tgcgacttcc ttgcccctac gctccacaac gacaccgctg ctttccgtat gagtcacgga
    32941 atcgaagcct gtccatatgg tgtacataaa ttcgccgttt tggaatttca tcgttgcccg
    33001 gcgtccgcta cccatgcctt gccacctgtc ggaacgcccc aacaggtcag ctttgctgtt
    33061 gcgtatggca atttcttttt ttgccgcact gccgaacgaa tattcgtaaa gattgcggtt
    33121 gattttttga acttctatat atttgttgtt gtccgtttta cagggaaaca ccgtatccgc
    33181 ccacacggtt tgggcggcag ccgacagaag caatacggca gaaaggaaca tttttttcat
    33241 cgtatttcct taatggttga aactccgcca ctcggacatc cgtccttcgt ggcagcagca
    33301 tcagatttta tttgagaggg gtgtaacccc ttccaaatta gggcaacaca tagggcaacg
    33361 ctttatgtgt cgtcctgtgt gttgaaacat cattgcccgt atcctgaaat agaaaagaaa
    33421 atgcccgttt tacggtcttc tttccgacaa aatgccgtct gaaacggatt tcagacggca
    33481 tggtcgaaaa ttattgcgcg gcaagcggct tgagcagcaa ggtttcgccg ctttgcacaa
    33541 ctttttcaag caggtcgata ttgggatgct tgccgggatg ggcgcgcgcg tcggcaacgt
    33601 gttcggcaaa ccatatcaaa ccctgttctg ccgcttcttt gtccaatttt ccgtcaaaac
    33661 gttctgccaa agcgttgtac aacttgagcg aaccgagctt accctgaacg gcaggaatgt
    33721 ggtgaaccgt tttgccatct gcatcctgaa cgtccaaacc gcccagatgg tcgattgaag
    33781 gaaatgtggc gagatagtct tgaaaattca tatcaactcc gaaaatcagt cagttagcgc
    33841 gtgccgaaaa tcttgtcgcc cgcatcgccc aagccgggga tgatgtagcc gttctcgttc
    33901 aagtggctgt cgagcgcggc ggtgtaaatc gtaacgtcgg gatgcgcgtc gttgaccgcc
    33961 ttcacaccct cgggcgcggc aaccagcacc agtgccttga tgtttttgca gcccttggct
    34021 ttcaaaaggt cgatggtggc aaccatcgaa ccgcccgtcg ccagcatcgg gtcgataatc
    34081 agggcaggac gttcgtccat gctgtccacg aatttctcga agtaggaaac gggtttcagc
    34141 gtttcctcgt cgcgctgcag tccgactaca ctgattttgg cagtcggaat caggtcgagt
    34201 acgccgtcaa gcatacccaa acctgcacgc agtatgggaa cgacggtcaa tgttttgccc
    34261 ttgatgcggt cgccttcaat ctgaccgcac catccgtcga taaggtattt ttcgatttca
    34321 aaatcacggc ttgcctcgta tgccatcagg cgcgccagct cgatggcaag cgtccggaat
    34381 ttgtaggtgc tgcaatccgc ctccctcatc agggttaatt tgtggcggac gagcggatgg
    34441 ttgataacat taacgttcat cgcggtgttc cagtgtgatt ttgagacgcg ccattatact
    34501 tttttttgat ttgccggaaa agaaaatgcc gtctgaaaca cgttcagacg gcattgccga
    34561 atccatcagg ctttcagcag ctcttgcagc tcgcctgcct catacatttc catcaggatg
    34621 tccgaaccgc cgacaaactc gccgttcaca taaagttggg ggatggtcgg ccagtcgctg
    34681 tattccttaa tgccttggcg cacttcggga ttttccaata cgttgacggt aacgtaatcg
    34741 gtgcagcctg ccgcgttcag gatttgcacg gcgcgggaag agaaaccgca ttgcggaaac
    34801 tgcttcgtac ctttcataaa caatacgacg cggtgtgtcg ttactacttc tttaatttgg
    34861 tcgtggatgg aagccatcgc ttattccttt tgataaagtt gacaaaattt gtcaggtagg
    34921 acgcattata cgcaaaagcg gaaacttccc acaatatttg ccgtatatcg cgcagtatca
    34981 atgttgtgaa cggaaatatt cccactcctc gacaatccta ctgtccggca aatggcacag
    35041 ggcatatccg ccgccgcttt cgtctttttt tacttcccgt ctgccgcctt tggcaatgca
    35101 gaatttggaa gcggaatttg ccatgcctgt ccgaaacgtg ttggcaggca gccggcagca
    35161 gcgcggacat gcatacggat gaattgcttt catggttttc cttatcgtcg gtacagggtc
    35221 agacgacccg catatcgggt acttttttaa acagccagta aaagttttcc gtcgtatatg
    35281 ccgcaacctg ttccaatgtt tggttccgca atttggcgat atgttcggcg gtatggcgca
    35341 caaaagccgg ttcgttctgc ctgccgcgtt tgggaacggg ggcgaggaac ggcgcatcgg
    35401 tttccaccaa aatgcggtcg tccggcacat attttgccgc ctcctgaacc aagggtgcgt
    35461 ttttaaaggt aacgattccc gagaaagaaa tataaagccc caaatccatt gctgcacggg
    35521 caaaaccgat gtcttcggaa aaacagtgga taacgcccga attaacccgg cattctttca
    35581 ggatagacaa ggtgtccgcc gccgcatcac gcgtatggac gataacgggc agtccggttt
    35641 gattggctgc ttcgatgtgg tctgcaaagc gtttgtgctg ccaggacaaa tcgcctttgc
    35701 accagtaata atccaaaccc gtctcgccaa tgccgaccac tttcggcgag gcggctgcgg
    35761 caaccatttc cgcaatagaa aattcttcgg cttccttgct gtcgggatgt acgcctatgg
    35821 tgcaatagat gtgttcgtgc gcttcggcga tggcaaagac ttcggagaag ctttccctac
    35881 tgacgctgat ggcgagcgcc tgccccacgc cgtttgcttc catgttggac aaaacttcgg
    35941 gcaggcgttc tttcaaacct tcaaaattga ggtggcagtg cgaatcgatg atatgcatgg
    36001 cgttacatgg taaacgtagg tctgctgccg ccgattgtgt tgcccagttc gacttcgatc
    36061 tggtctttga ctttcaggca gttttcgtgt tttgtgaatg ccagccccac ccctttgggt
    36121 ttggaggaag tacgcgcagg attgatccag gcgactttgg tcggcaggaa cagtttgggg
    36181 aagttgagga tttctacggc aagcagaata tcgtccccga tggaaaatac gtcgttggtc
    36241 tgcacaaaca gaccgccgtg ttccaaaaac ggcatatagg agctgtacag cagattcatg
    36301 tctttcagct gcaacgacat catttttgcc ggaatatttt gtccgtctga cataattcac
    36361 ccgttctctt tcttcaattc caaataattg atgagcagat gctcgatctg cattttgaca
    36421 tttaaagtat gaaacccgta aggggcaagc tgtttgagca tatcctccgc cgcaaataca
    36481 ttgcgcggac ggaaaccgga taccgtctga agcagcctgt cttcataagc gggataatag
    36541 acgggtttca tgtgttgcag gcacaatccc aaatcgacca gccatttctg catccacccg
    36601 acaaatacgg cgagcggaag tttttcctta tcgaaaagcg cggcgtaatc caaaatcttc
    36661 aacaaccttg gttctgccaa aatatccaac agtttaatcc gcaacgcacg gacaccgtcc
    36721 gcctcatcaa acagcggcgc tccggaatgg aaagccagac gttcctcagg ttccgccaca
    36781 cccctttcac gcagatatgc caatgcctct tcatgggaag gcgcgggcaa aaccatcttc
    36841 cggcagcggc ttttaatggt cggtaaaacc ttgtccgccg cgtggctgac cagcaaaaag
    36901 accacttgtg gcggcggctc ttccaacact ttcaacaaac tgttggcggc ttggacattc
    36961 atactttccg caggatgaat cagaatcacg cgcaaaccgc cccgtaccga agtcaggtac
    37021 acattatcga tgatttccct gacggcatcg attttgatct gcaacagttt gcgtccgttt
    37081 tcgggttcgt ccgacaaggg ggtgatttcg taaaaatcgg gatggcttcc ctgtccaaac
    37141 agatggcagg acatacattc gccacagggt ttgcagcccg gtgcaggggt ttcgcacaac
    37201 agtgccttcg ccgcaaagcg ggcaaatgta gttttccccg tatctttttt gccggcaaac
    37261 agccatgcat tgggacgacg ctcccaatgt tccgcaatct gccgccattg ctcattatgc
    37321 cacggataaa tcatttcaaa ccatccgtcc ggaaatccat accgccatca gagcagttcc
    37381 aaatcggggc tggaactttg cgcgcggcgg ctgcgcaact gtaccgccaa acgcaaatcg
    37441 tgtgcggaat cggcattttt caatgcttct tgcagggaaa tatcgccttt ttcatacaat
    37501 tggtaaaggt gttgatcgaa ggtctgcata cccagggtag tggatttttt catcacttct
    37561 ttgatttcat ggatgttgcc gttgtgaatc aactccgaaa tcaggggcga attgagcagc
    37621 acctcgactg ccgccaccct gcccttgccg ccgtctcgcg gaacgaggcg ttgcgaaata
    37681 aacgcctgaa ggttgagcga caaatccgtc agcaattgtt cgcgccgctc ctccgggaaa
    37741 aagttgatga tgcggtcgag tgcctgattg gtgctgttgg cgtgcagcgt cgccatacac
    37801 aaatgccccg tttcggcaaa ggcaatggcg tagtccattg tttcgcggtc gcggatttcg
    37861 ccgataagga tgacatcggg cgcctgacgc agcgtgtttt tcaacgccgc catccagttt
    37921 tccgtatcca cgccgacctc tcgctgggtg atgatgcagt ttttgtgttc gtggacaaac
    37981 tcgatcgggt cttcgatggt gataatgtgt ccgaacgaat tttcattgcg gtaatcgata
    38041 agcgaggcaa gcgaagtcga tttgcccgag ccggtgccgc cgacaaaaat aaccagcccg
    38101 cgttttttca gcgcgacatc cttcaagacc ggcggcaggt tgaggctttc aaacttggga
    38161 atcttgctgg taatcgcacg gaataccaac gccgtcgcgc cgcgctgtat catcgcattg
    38221 acgcggaagc ggctggtgtc cggcaggctg atggcgaagt tgcactcgtt ggtcgatgaa
    38281 aattcttccg cctgcttcgc actcataatc gaaaaggcga tttccataca tttttccgcc
    38341 gtcagcggtt cgtccgtgat gcgggtgatt ttgccgtcca gcttcatcgc gggcgggaaa
    38401 tgggtcgtca caaacaggtc ggaacctttg tttttgttca tatggcgcag ccatgcgaac
    38461 agttcttcct ttgcggtcat attttcatcg gtaaacatcc tcgtccccta atcttcaaac
    38521 aatctgtacc ttcccattct accaaaa
//
LOCUS       DAXIEB010000004        38278 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000004 DAXIEB010000000
VERSION     DAXIEB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38278
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.004"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            55..2190
                     /gene="fetA"
                     /locus_tag="ACFOA3_000187"
                     /gene_synonym="frpB"
     CDS             55..2190
                     /gene="fetA"
                     /locus_tag="ACFOA3_000187"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6897266.1"
                     /translation="MNTPFFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQHMTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGYKSPNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTIFDPDPAKDRQK
                     PSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKGSGYAGNVEGPNHTR
                     ITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFSIKDKDGATETDKQKNR
                     EAEKIAKAYRLTNPTKTDAGVYVEAIHDIGDFTLTGGLRYDRFKVKTHDGKTVSSSNL
                     NPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNTEI
                     GFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASYRT
                     GGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIGWRG
                     RYVQKATGSILAAGQRNRDKQLENVVRKGFGVNDVFANWKPLGKDTLNVNLSVNNVFN
                     KYYYPHSQRWNNTLPGVGRDVRLGVNYKF"
     gene            2348..3313
                     /locus_tag="ACFOA3_000188"
     CDS             2348..3313
                     /locus_tag="ACFOA3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6897267.1"
                     /translation="MLRLTALTLCTALALGACSPQNSDSAPQAKEQAVSAAQSEGVSV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3642..4610
                     /locus_tag="ACFOA3_000189"
     CDS             3642..4610
                     /locus_tag="ACFOA3_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6897268.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADRLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4600..5574
                     /locus_tag="ACFOA3_000190"
     CDS             4600..5574
                     /locus_tag="ACFOA3_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6897269.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLLVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5619..6245
                     /locus_tag="ACFOA3_000191"
     CDS             5619..6245
                     /locus_tag="ACFOA3_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897270.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALCRRDTGKTVAVSLNAALQPFAPQ
                     MRNIMPKAVSGSASADELKQFGGLWQERVRAFLIDQADNPEFVTVSEI"
     gene            6329..7087
                     /locus_tag="ACFOA3_000192"
     CDS             6329..7087
                     /locus_tag="ACFOA3_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6897271.1"
                     /translation="MITIRNVSFHIDTRPILDNISLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPSETDKTIVEEALAEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPNDIFTAENIKTLFDMDVNVLDYEGKKLIVHHI"
     gene            complement(7146..7484)
                     /locus_tag="ACFOA3_000193"
     CDS             complement(7146..7484)
                     /locus_tag="ACFOA3_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6897272.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            7579..7791
                     /locus_tag="ACFOA3_000194"
     CDS             7579..7791
                     /locus_tag="ACFOA3_000194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897273.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            7948..11904
                     /gene="purL"
                     /locus_tag="ACFOA3_000195"
     CDS             7948..11904
                     /gene="purL"
                     /locus_tag="ACFOA3_000195"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6897274.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKTLDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVEDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHHPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQQDRDYVDAAFKAAD
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRIHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKSAS
                     LILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
     gene            11999..12757
                     /gene="gloB"
                     /locus_tag="ACFOA3_000196"
     CDS             11999..12757
                     /gene="gloB"
                     /locus_tag="ACFOA3_000196"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6897275.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGEGLITVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAEHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            13064..17542
                     /gene="mspA"
                     /locus_tag="ACFOA3_000197"
                     /gene_synonym="ausI"
     CDS             13064..17542
                     /gene="mspA"
                     /locus_tag="ACFOA3_000197"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6897276.1"
                     /translation="MRFTHTIPFYSVLSTLGLFAVSPAYSSIVRSDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLSGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAVAGDSGSPLFAFDKHENRWVLAGVLGTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGGSTLQGLNEHITLPIANPSLAPQN
                     DSRHMPSADAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPILSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPVPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPPLKLEGDSRGAFQ
                     IHVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYN
                     PLKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELAR
                     IDSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETEL
                     DTYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPN
                     LAELISRSANTAVSEQAAYNTGRQQAERRIDRHLTDPQQQNIWLETGTQQTDYHSGTH
                     RPYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGEN
                     GALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRS
                     NGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSAL
                     SVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            17774..19228
                     /gene="mgtE"
                     /locus_tag="ACFOA3_000198"
     CDS             17774..19228
                     /gene="mgtE"
                     /locus_tag="ACFOA3_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6897277.1"
                     /translation="MSIEQTPPNLENNGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(19314..20222)
                     /gene="hslO"
                     /locus_tag="ACFOA3_000199"
     CDS             complement(19314..20222)
                     /gene="hslO"
                     /locus_tag="ACFOA3_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6897278.1"
                     /translation="MNQTAIDRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            20467..21222
                     /locus_tag="ACFOA3_000200"
     CDS             20467..21222
                     /locus_tag="ACFOA3_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6897279.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            21241..21459
                     /locus_tag="ACFOA3_000201"
     CDS             21241..21459
                     /locus_tag="ACFOA3_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897280.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            21604..21951
                     /locus_tag="ACFOA3_000202"
     CDS             21604..21951
                     /locus_tag="ACFOA3_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6897281.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            21948..22640
                     /locus_tag="ACFOA3_000203"
     CDS             21948..22640
                     /locus_tag="ACFOA3_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6897282.1"
                     /translation="MNEILRQPSVLLFLTLAVYAIAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFLDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            22707..23927
                     /gene="argJ"
                     /locus_tag="ACFOA3_000204"
     CDS             22707..23927
                     /gene="argJ"
                     /locus_tag="ACFOA3_000204"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6897283.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            23987..25396
                     /locus_tag="ACFOA3_000205"
     CDS             23987..25396
                     /locus_tag="ACFOA3_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6897284.1"
                     /translation="MTSTFPRRLARKIRQTRRLSLKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGATVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            25586..26977
                     /gene="hrpA"
                     /locus_tag="ACFOA3_000206"
     CDS             25586..26977
                     /gene="hrpA"
                     /locus_tag="ACFOA3_000206"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6897285.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYQKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYSPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPTGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            27078..28205
                     /locus_tag="ACFOA3_000207"
     CDS             27078..28205
                     /locus_tag="ACFOA3_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6897286.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALRENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            28383..29960
                     /gene="lpt3"
                     /locus_tag="ACFOA3_000208"
     CDS             28383..29960
                     /gene="lpt3"
                     /locus_tag="ACFOA3_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6897287.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFSRYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEANIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            30062..33487
                     /locus_tag="ACFOA3_000209"
     CDS             30062..33487
                     /locus_tag="ACFOA3_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6897288.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALLIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSENQGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQEREQSAS
                     ASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATTANPLPNPLPQEGEQSAA
                     ASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDLMQHA
                     AAHITEEQFPKFWQTADGKFELSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEWLVPG
                     MLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAIAKTAGDIR
                     ILEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNT
                     QEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTTEAAEQAH
                     RQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGED
                     ELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNSKLGKHPLTHLLRLRL
                     QTLLAAGFASHTPWVQWPRLPIYLKAMTLRLEKYSSNPARDASREADIQELEQMWQEK
                     NDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLLKMWEDLN"
     gene            complement(33510..33932)
                     /locus_tag="ACFOA3_000210"
     CDS             complement(33510..33932)
                     /locus_tag="ACFOA3_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC6897289.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(33936..35582)
                     /locus_tag="ACFOA3_000211"
     CDS             complement(33936..35582)
                     /locus_tag="ACFOA3_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC6897290.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(35597..36652)
                     /locus_tag="ACFOA3_000212"
     CDS             complement(35597..36652)
                     /locus_tag="ACFOA3_000212"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6897291.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESGLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITEEKLEPAKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            36942..37094
                     /locus_tag="ACFOA3_000213"
     CDS             36942..37094
                     /locus_tag="ACFOA3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897292.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            37351..37619
                     /locus_tag="ACFOA3_000214"
                     /pseudo
     CDS             37351..37619
                     /locus_tag="ACFOA3_000214"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(37709..38158)
                     /locus_tag="ACFOA3_000215"
     CDS             complement(37709..38158)
                     /locus_tag="ACFOA3_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6897293.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         9606 a        11343 c         9027 g         8302 t
ORIGIN      
        1 caaatcttaa cggtttggct taacttccct tcatacactc aagaggacga ttgaatgaat
       61 accccgtttt tccgtctcag cctgctctcg ctcacacttg cggcaggttt tgcccacgcg
      121 gcagaaaata atgccaaggt cgtactggat accgttaccg taaaaggcga ccgtcaaggc
      181 agcaaaatcc gtaccaacat cgttacgctt caacaaaaag acgaaagcac cgcaaccgat
      241 atgcgcgaac tcttaaaaga agagccatcc atcgatttcg gcggcggcaa cggcacgtcc
      301 caacatatga ccttgcgcgg tatgggtcag aactctgtcg atatcaaggt ggacaacgcc
      361 tattccgaca gccaaatcct ttaccaccaa ggcagattta ttgtcgatcc cgctttggtt
      421 aaagtcgttt ccgtacaaaa aggcgcgggt tccgcctctg ccggtatcgg cgcgaccaac
      481 ggcgcgatta tcgccaaaac cgtcgatgcc caagacctgc tcaaaggctt ggataaaaac
      541 tggggcgtgc gcctcaacag cggctttgcc agcaacgaag gcgtaagcta cggcgcaagc
      601 gtattcggaa aagagggcaa cttcgacggc ttgttctctt acaaccgcaa cgatgaaaaa
      661 gattacgaag caggtaaagg ctacaaaagt cccaacggcg gcaaaaccgt accgtacagc
      721 gcgctggaca aacgcagcta cctcgccaaa atcggaacaa gcttcggcga cggcgaccac
      781 cgcatcgtgt tgagccatat gaaagaccaa caccggggca tccgcactgt gcgtgaagaa
      841 ttcacaatct tcgatcccga tcctgcaaaa gacaggcaga aaccctcata ccgtgaaact
      901 acccaatcca acaccaactt ggcgtacacc ggcaaagatt tgggctttgt cgaaaaactg
      961 gatgccaacg cctatgtgtt ggaaaagaaa cgctattccg ccgatgacaa aggcagcggt
     1021 tacgcaggca atgtagaagg ccccaaccat acccgcatta ccactcgggg tatgaatttc
     1081 aacttcgaca gccgccttgc cgaacaaacc ctgttgaaat acggtatcaa ctaccgccat
     1141 caggaaatca aaccgcaagc gtttttgaac tcgcaatttt caataaaaga taaagatggc
     1201 gcaaccgaga cagacaagca aaaaaaccgt gaagctgaaa aaattgccaa agcctaccgt
     1261 ctgaccaacc cgaccaaaac cgatgccggc gtatatgttg aagccattca cgacatcggc
     1321 gatttcaccc tgaccggcgg gctgcgttac gaccgcttca aggtgaaaac ccacgacggc
     1381 aaaaccgttt caagcagcaa ccttaacccg agtttcggcg tgatttggca gccgcacgaa
     1441 cactggagct tcagcgcgag ccacaactac gccagccgca gcccgcgcct gtatgacgcg
     1501 ctgcaaaccc acggtaaacg cggcatcatc tcgattgccg acggcaccaa agccgaacgc
     1561 gcgcgcaata ccgaaatcgg cttcaactac aacgacggca catttgccgc aaacggcagc
     1621 tacttctggc agaccatcaa agacgcgctt gccaatccgc aaaaccgcca cgactctgtc
     1681 gccgtccgtg aagccgtcaa cgccggttac atcaaaaacc acggttacga attgggcgcg
     1741 tcctaccgca ccggcggcct gactgccaaa gtcggtgtca gccacagcaa accgcgcttt
     1801 tacgatacgc acaaagacaa gctgttgagc gcgaaccctg aatttggcgc acaagtcggc
     1861 cgcacttgga cagcttccct tgcctaccgc ttcaaaaatc cgaatctgga aatcggctgg
     1921 cgcggccgtt atgttcaaaa agctacgggt tcgatattgg cggcaggtca aagaaaccgc
     1981 gacaaacaat tggaaaacgt tgtacgcaaa ggtttcggtg tgaacgatgt cttcgccaac
     2041 tggaaaccgc tgggcaaaga cacgctcaat gtcaatcttt cggttaacaa cgtgttcaac
     2101 aagtactact atccgcacag ccaacgctgg aacaataccc tgccgggcgt aggccgcgat
     2161 gtacgcttgg gcgtgaacta caagttctaa aacgcacatc ccgaaaaaat gccgtctgaa
     2221 agcctttcag acggcatctg tcctgatttt tgttaatcca ctatagatta tcatttatcc
     2281 tttctaaagc cgttccggtt tgtccgaccg acggctttgc cccaatatcc ccattttgga
     2341 gacacctatg ttgcgtttga ccgcattgac cctctgcacc gccctcgcct tgggcgcgtg
     2401 ttcgccgcaa aattccgact ctgccccaca agccaaagaa caggcagttt ccgccgcaca
     2461 atccgaaggc gtgtccgtta ccgtcaaaac cgcgcgcggc gatgttcaaa taccgcaaaa
     2521 ccccgaacgt atcgccgttt acgatttggg tatgctcgac accttgagca aactgggcgt
     2581 gaaaaccggt ttgtccgtcg ataaaaaccg cctgccgtat ttagaggaat atttcaaaac
     2641 gacaaaacct gccggaactt tgtttgagcc ggattacgaa gccttaaacg cctacaagcc
     2701 gcagctcatc atcatcggca gccgcgcagc caaagcgttt gacaaattga acgaaatcgc
     2761 gccgaccatc gaaatgaccg ccgataccgc caacctcaaa gaaagtgcca aagagcgtat
     2821 cgacgcgctg gcgcaaatct tcggcaaaca ggcggaagcc gacaagctga aggcggaaat
     2881 cgacgcgtct tttgaagccg cgaaaactgc cgcgcaaggc aaaggcaaag gtttggtgat
     2941 tttggtcaac ggcggcaaga tgtccgcctt cggcccgtct tcacgactgg gcggctggct
     3001 gcacaaagac atcggcgttc cagccgttga cgaagccatt aaagaaggca gccacggcca
     3061 gcctatcagc tttgaatacc tgaaagagaa aaatcccgac tggctgtttg tccttgaccg
     3121 cagcgcggcc atcggcgaag agggtcaggc ggcgaaagac gtgttggata atccgctggt
     3181 tgccgaaaca accgcctgga aaaaaggaca ggtcgtgtac ctcgttcctg aaacttattt
     3241 ggcagccggc ggcgcgcaag agctgctgaa tgcaagcaaa caggttgcgg atgcttttaa
     3301 cgcggcaaaa taatgtttca acacacaggg cggtacataa agcaccgccc tatgtgtcgt
     3361 cctgatttgg aaggggttac gcccctccca aatatagtgg attaaattta aaccagtacg
     3421 gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     3481 ttttgttaat ccactatatc gcgggaatgg cggcaagcgt ttttattgga acttgcccta
     3541 tcctctgccc gatacggagg cttcaaacaa ggctttccgc tccgacggtt cggactgcct
     3601 tgtttgaatc ttctacgcct taacgctttt cccttctgtt tatgactgcc aaaccttttt
     3661 ccctcaacct gaccaacctc ctgctgctgg cggtattgtt tgccgtcagc ctgtcggtgg
     3721 gcgttgccga tttccgctgg tcggatgtgt tttcgctatc cgacagccag caggtcatgt
     3781 tcatcagccg cctgccgcgc acgtttgcga ttgtgttgac gggcgcgtcg atggcggtgg
     3841 cggggatgat tatgcagatt ctgatgcgta accgttttgt cgagccttct atggcgggcg
     3901 cgggtcagag tgcggctttg ggtttgcttc tgatgtccct gctgctgcct gccgcgccgc
     3961 tgccggtcaa aatgtcggtt gccgccgttg ccgcgttaat cgggatgttg gtgtttatga
     4021 tgcttatccg ccgcctgccg ccgacggcgc aactgatggt gcctttggtc gggattattt
     4081 tcggcggcgt ggttgaggcg gtggccacct ttattgcgta tgaaaacgaa atgctgcaaa
     4141 tgctgggcgt gtggcaacag ggcgattttt ccggcgtgtt gctcggacgg tatgaactgt
     4201 tgtgggcaac ggggattttg gctttgtttg cctatttgat tgccgaccgg ctaacgattt
     4261 tgggtttggg cgaaacggta agcgtgaact tggggctgaa ccggacggcg attctgtggt
     4321 cggggctgat tattgtggct ttgattacgt cgctggttat cgttacggtc ggcaatattc
     4381 cgtttatcgg gctggtcgtg ccgaacatca tcagccgcct gataggcgac aggctgcgcc
     4441 aaagcctgcc tgcggtggct ttgttgggcg cgtctttggt gttgctgtgc gacattatcg
     4501 gacgcgtgat tgtgtttccg tttgaaattc cggtatcgac cgtcttcggc gtattgggta
     4561 cggcgttgtt tttatggctt ttgttaagga aacctgctca tgccgtctga aaaaaatatc
     4621 ggttttatgg caggaagcag ccgtccgttg tgggttgcct ttgcgctgtt gctggtttcc
     4681 tgcatcctgt ttatgacgct caacgtcaaa ggcgattggg attttgtact gcacctgcgg
     4741 ctgaccaagc tcgcggcttt acttttagtc gcctatgcgg tcggcgtgtc cactcaactc
     4801 ttccaaacgc tgaccaacaa tccgattctg accccttcga ttttgggttt cgattcgctg
     4861 tatgtgtttt tgcagacctt gctggtgttt acgttcggcg gcgtgggcta tgcttccctg
     4921 ccgttgacgg gcaaattcgg ctttgaactg gtcgttatga tgggcggctc gctgctgctg
     4981 ttctacacgc ttatcaaaca gggcggacgc gatttgccgc gtatgatttt aatcggcgtg
     5041 attttcggga ttttgttccg cagcctgtcg tcgctgcttt cgcgcatgat cgaccccgaa
     5101 gaatttacgg cggcgcaggc gaatatgttt gccggattca ataccgtcca cagcgagctt
     5161 ttgggcatag gcgcgctgat tctgctcgtc agcgcggcgg tcgtttggcg cgaacgctac
     5221 cgcttggacg tacacctttt ggggcgcgac caagccgtca atttgggcat cagctacacg
     5281 cgcaacacct tatggatact gctttggatt gccgcattgg tggcgacggc gaccgccgtg
     5341 gtcggtccgg tgagcttttt cgggcttctc gccgcctcgc tggccaacca cttttccccg
     5401 tccgtgcgcc attccgtccg cctgccgatg acggtttgtg tcggcggcat cctcttggtc
     5461 ggcggacaaa ccgtattcga acacttcttg ggcatgaagg cggtattaag cgtggtggtc
     5521 gaatttgcgg gcggactcgt tttcctctat ctcgttttaa aacacaaaaa atgacggatg
     5581 ccgtctgaac ggcagcccgc cccgaaagga caaaccatat gacgcaagaa cgtttacccg
     5641 aatttttcga ccgcgccccg acgctgaccg tacaagaccc gcttgccgca ttcctcggcg
     5701 cggcgcaaga cggcatcctc acttaccgct acgccgatgc cgtgcgcctg tgcggacatt
     5761 cctgcccgac cgtcgcgggc gcgtacctga tggttatcaa aggtctgaaa gccctttacg
     5821 gcgaagagct gcccgaacgc ggcggcatcg aagccgccat gcagggaacg cgcgacgaag
     5881 gcacggtcgg cgtaaccgcg tccgtcgtcc aactcctcac cggcgcagcc cccgaaaccg
     5941 gcttcggagg catcggaatg cagggacgct tcgcccgccg caacctcttg tcctttggtg
     6001 caggcgaaat caacggcaca ctcgcactct gccgccgcga taccggcaaa accgttgccg
     6061 tcagcctcaa cgccgcgctc caaccttttg caccgcaaat gcgcaacatc atgcccaaag
     6121 ccgtcagcgg cagcgcaagt gccgacgaac tcaaacaatt cggcgggctt tggcaagaac
     6181 gcgtccgcgc cttcctgatt gaccaagccg acaatcccga atttgtcacc gtcagcgaaa
     6241 tctaaccaca ccatacagga ataaaattca gacggcctca cgctgaaatg ccgtctgaaa
     6301 acccaatttt ccatacccga atccgcctat gattaccatc cgcaacgtca gcttccacat
     6361 cgacactcgc cccattctgg acaacatcag cctcgacatt cccgaaggcg gtattaccgc
     6421 gctgatcggc ccgaacggcg caggcaagtc caccctcctc tcctttatgg cgcggcttca
     6481 gcctctggta cacggcaaca tcagctacgc aggcaaagac atcaaaacca cccctaccgc
     6541 cgaactcgcc cgcacgctct ccatcctcac ccaagaaaac agcatcatga gccgcattac
     6601 cgtgcgcgac ctgctcatgt tcggccgcta tccctaccac caaggcaggc cgtctgaaac
     6661 ggataaaacc attgtcgaag aagcactcgc cgagttccac ctacaagact tcgccgaccg
     6721 ctacctgacc gagctttccg gcggccaacg ccaacgcgcc atgattgcca tggtattctg
     6781 ccagcgcacc gactacgttt tgctggacga accgctgaac aacctcgaca tgtatcatgc
     6841 ccgctccctc atgcaaatcc tgcgccggct gaccgatgaa cacaaacgca ctaccgtcgt
     6901 cgtattgcac gacatcaacc aggccgccgc ctacgccgac cacgtcgtcg ccatgaaaaa
     6961 cggccaagtc gccatgcagg gcaagcccaa cgatattttc actgccgaaa acatcaaaac
     7021 cttattcgat atggacgtca acgtcctcga ttacgaaggc aaaaaactca tcgtccacca
     7081 tatctgaagc tcaaaaagaa aatgccgtct gaaaaccttt cagacggcat ttccattcca
     7141 aacgctcaaa ccgccgcgtc cgaacgttcg cccgtgcgga tacggattgc ctcctcgacc
     7201 ggcagcacaa aaatcttgcc gtcgccgatt ttgcccgaac gcgccacctc gacaatcacg
     7261 tcaatcgcgc gttccacagc gtcatccgcc aacaccagct cgattttgac cttgggcagg
     7321 aaatcgacgg cgtattccgc accgcgatag atttccgtat gccccttctg cctgccgaac
     7381 cctttgacct cgctgacggt catccccgta atgccgattt ccgtcaacgc ctcgcgcacg
     7441 tcgtcgagtt tgaacggttt gacaatcgcc tcgatttttt tcatcacatt ctccattaat
     7501 taacgtaaac aaacgacaaa tatagcagaa accgccggca aacgataaac ggtactgttc
     7561 gcccggcgat gcggcgattt gccggaaacc gtcattcccg cgcaggcggg aatcccgatc
     7621 tctgcatttc agcaatattt aaaggtttct gtaaatccaa acttccatat tcctaaccgt
     7681 gcggaaacga cggcatttga tctaaaaaca tacggaagca gttgcccgcc cctctacttc
     7741 ctgcaattat ccgcataccc ggctttgggt aaaaatcaga aaaacagata aaacccttat
     7801 tccttaatat cttcagacgg cacctgtatt ttccccaact gtatgccgtc tgaagattgt
     7861 caacatttta agccaaaata tccaagcaat acaaacctgt ccggcgtata atccctcttt
     7921 ttcccaaccg catttgagag ccgaattatg tctgttgtgt tgcccttgcg cggcgttacc
     7981 gccctttccg atttccgtgt tgaaaaactc ttgcaaaaag ccgccgcact cggtctgccc
     8041 gaagtcaaat taagcagcga attttggtat ttcgtcggca gcgagaaaac actggatgcc
     8101 gcaactgtcg aaaaactgca agccttactg gcagcgcaaa gcgttgaaca aacgcccgaa
     8161 gcgcgcgagg gcttgcattt atttttggtc acgccccgtt tgggtacgat ttcgccttgg
     8221 gcttccaagg caaccaatat cgcggaaaac tgcggtttgg caggcatcga gcgcatcgag
     8281 cgcggcatgg cggtgtggct ggaaggtgcg cttaccgatg aacagaaaca gcaatgggcg
     8341 gctttgctgc acgaccgcat gaccgaatcc gtattgcccg attttcagac ggcatccaaa
     8401 ttattccacc atctcgaatc cgaaaccttt tccaccgttg atgttttagg cggcggtaaa
     8461 gaggctttgg tcaaagccaa taccgaaatg ggtttggcac tctccgccga cgaaatcgat
     8521 tatctggttg aaaactatca ggctttgcag cgcaatccgt ccgatgttga attgatgatg
     8581 ttcgcgcagg caaacagcga acactgccgc cacaaaatct tcaacgccga tttcatcctc
     8641 aacggcgaaa aacaaccgaa atccctgttc ggcatgatac gcgacacgca caacgcgcat
     8701 cccgaaggta cggtcgtcgc ttataaagac aattcgtccg taatcgaagg cgcgaaaatc
     8761 gagcgtttct atccggatgc ggcggaaaac caaggctacc gtttccacga ggaagacacg
     8821 catatcatca tgaaagtgga aacgcacaac cacccgaccg ccatcgcgcc gtttgcgggc
     8881 gcggcgacgg gcgcgggcgg cgaaatccgc gacgaaggcg caacgggcaa aggttcgcgt
     8941 ccgaaagcag gcttgaccgg ctttaccgtg tccaacctca acatccccgg cctcaaacag
     9001 ccgtgggaac aagattacgg caagccggaa catatttcct cgccgctgga cattatgatt
     9061 gaaggcccca tcggcggcgc ggcgttcaac aacgaattcg gccgccccaa cctcttgggc
     9121 tacttccgca cctttgaaga aaaatttgac ggtcaggttc gcggctatca caaaccgatt
     9181 atgattgctg gcggtctggg cagcattcag gcgcagcaga cgcataaaga cgaaatcccc
     9241 gaaggcgcgt tgctgatcca actgggcggc ccgggtatgc ttatcggctt gggcggcggc
     9301 gcggcttctt cgatggatac cggtacaaat gacgcgtctt tggacttcaa ctccgtgcaa
     9361 cgcggcaacc ccgaaatcga acgccgcgcg caggaagtga tcgaccgctg ctggcagctc
     9421 ggcggcaaaa acccgattat ctccatccac gacgtgggcg cgggcggttt gtccaacgcc
     9481 ttccccgaac tcgtcaacga tgccggacgc ggcgcggtat tcaagctgcg cgaagtgccg
     9541 ttggaagaac acggtttaac gcctttgcaa atctggtgta acgaatcgca agagcgttat
     9601 gtgttgtcga ttttggaaaa agatttggac accttccgcg ccatctgcga acgcgaacgc
     9661 tgcccgtttg ccgtggtcgg cacggcgacc gacgacggcc atttgaaagt gcgcgacgat
     9721 ttgttcgcca acaaccccgt cgatttgccg ttgaacgtct tgctcggcaa accgcccaaa
     9781 accacgcgta ccgacaaaac agttacgcca tctaaaaaac cgtttcacgc cgacgatatc
     9841 gacatcaccg aagccgccta ccgcgttttg cgcctgcctg ccgtagccgc caaaaacttc
     9901 ctgattacca tcggcgaccg cagcgtcggc ggcatgaccc accgcgacca aatggtcggc
     9961 aaataccaaa cccccgtagc cgactgcgcc gttaccatga tgggcttcaa tacctatcgc
    10021 ggcgaagcaa tgtctatggg cgaaaaaccg accgttgccc tgtttgacgc gcccgcctcg
    10081 ggcagaatgt gcgtcggcga agccatcacc aacatcgcgg cagccaatat cggcgacatc
    10141 ggcaacatca agctctccgc caactggatg gcggcgtgcg gcaacgaagg cgaagacgaa
    10201 aaactctacc gcaccgtcga agcggtttcc aaagcctgtc aggcattgga tttgagcatc
    10261 cccgtgggca aagacagcct gtcgatgaaa actgtgtggc aggacggcga agagaaaaaa
    10321 tccgtggttt cgcccttgag cctgattatc tccgcgtttg ctccagtgga agacgtacgc
    10381 aagactgtta cacccgagtt gaaaaacgtc gaagacagcg tattgctgtt tgtcgatttg
    10441 ggcttcggca aagcgcgtat gggcggctcg gcgttcggtc aggtgtacaa caatatgagc
    10501 ggcgacgcgc ccgatttgga cgacacaggc cgtctgaaag cgttttacaa cgtgattcaa
    10561 cagcttgtcg ccgaagacaa acttttggcg tatcacgacc gcagcgacgg cggtttgttt
    10621 gcgacactgg cggaaatggc gtttgcggca cggtgcggta tcagtgccga tatagattgc
    10681 ctgatggata aattcctgcc gattcatcac ccggatttcc aaggcgaccc ggccgaggac
    10741 ttatctgacg aactttataa tcatgccgcc attaaaatct tattcaatga agaattaggt
    10801 gcggttatcc aaatccgcca acaagatagg gattatgttg atgcggcatt taaagcggcc
    10861 gacttaaccg atgcggtcag ccggattggc tctcctgatt ttgacaatga gtttatttct
    10921 ttctttggtt acggctattt cttggaacaa aaccgcgccg acctgcaacg cgcatggcag
    10981 gaaaccagcc acgccatcca acgcctgcgc gacaaccctg cctgcgccga cagcgagttc
    11041 gcattgattg gcgacaacgg acgcagcgca ttgtttgcca acctgaaatt cgacgtgaac
    11101 gaagacatcg ccgcgccgtt tatcaacagc ggcgcgaaac ccaaaatcgc catcctgcgc
    11161 gaacagggcg taaacgggca aatcgaaatg gccgccgcct ttacccgcgc cggattcgat
    11221 gcttacgacg tgcatatgtc cgacctgatg gcaggccgca tccacctcgc cgacttcaaa
    11281 atgctggcgg cgtgcggcgg cttcagctac ggcgacgtac tcggcgcggg cgaaggctgg
    11341 gcgaaatcga ttctgttcca ccctgctctg cgcgaccagt ttgccgcctt cttcgccgac
    11401 ccggacacgc tgacattggg cgtgtgcaac ggctgccaaa tggtcagcaa ccttgccgaa
    11461 atcatccccg gcacggcagg ctggccgaag ttcaaacgca acctgagcga acagtttgaa
    11521 gcgcgcctaa gcatggttca cgtccccaaa tccgcgtcgc tgattctgaa cgaaatgcaa
    11581 ggctccagcc tgcccgtcgt cgtcagccac ggcgaaggcc gcgccgactt cgcacttcac
    11641 ggcggcaaca tttctgccga tttgggcatt gcgctgcaat atgtggacgg tcaaaaccaa
    11701 gttacccaaa cctatccgct caaccccaac ggctcgccgc aaggcatcgc cggcattacc
    11761 aacgccgacg gccgcgtgac catcatgatg ccccaccccg aacgcgtgta ccgcgccgca
    11821 caaatgagct ggaaaccgga aggctggacg gaactgtccg gctggtaccg cctctttgcc
    11881 ggcgcacgta aagccttggg ctaaccgccc tactcaaacc aatgccgcct gaagaatatt
    11941 tcagacggcg ttccggcata ccatccttta aacggtatcc gtccaccgag gaacactcat
    12001 gaaaatcacc cccgtcaaag ccctaaccga caactacatc tggatgatac agcacggcaa
    12061 ccatgccgtc tgcgtcgacc cttccgaacc ctcgcccgtc ttggaattcc tcgtccgcaa
    12121 ccgcctcatg cttgcccaaa catgggtaac gcacccccat cccgaccacg agggcggtgc
    12181 ggcggcactc tggcgcggct acatggaatc gcccgtttac ggcgaatccg acatcgaagc
    12241 agcaacccac accgtaaccg ccggcaccca attcaccttc ggcgaagggt taatcaccgt
    12301 ttgggcaacc ccgggccaca cagaccgcca caccagctac cttctcgaaa cttcagacgg
    12361 catacacgtc ttttgcggcg acaccctttt ttccgccggc tgcggacgcg tgtttaccgg
    12421 cacaatcgaa cagctttacg acagcttcca acgcttcaac caattacccg aaggcaccct
    12481 gttctacccg gcgcacgaat acaccgccgc caacctgcgt ttcgccgccc atatcgagcc
    12541 ggacaacgcc gacattcaga cggcactgaa agcggcagaa cacacgccta ccctgcccgt
    12601 taccctcgcg cacgaacgcc gcgtcaaccc gtttttgcgc gtcgacctgc cgcacgtcag
    12661 agaccgcgcc gaggcattga gcgggaaaac gttaaacagc agcctcgata cctttgtcgc
    12721 gctgcgtgaa cttaaaaacc aataccggac gaaataaaac aacgggaaaa cgcagccatt
    12781 cctaggattt ttattaaaat cttaaataaa atcatacaat catcgccaat agacgaaagg
    12841 acaccgttgc cttataatca aacaaaaaca aaatatataa tatagtggat tgaatttaaa
    12901 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgcc
    12961 gtactggttt ttgttaatcc actatataaa tccagcacaa aacgggatcg gtgattcttg
    13021 tccgcaagaa tcgttgattt tctctattac acggataatc atcatgcgct tcacacatac
    13081 catcccattt tattccgtat tgtccaccct cggtcttttt gccgtttccc ctgcttactc
    13141 atccattgtc cgcagcgatg tcgattacca atattttcgc gactttgccg aaaataaagg
    13201 cgcgttcgcc gtaggtgcaa gcaatatttc catccaagac aagcaaggca aaatattagg
    13261 cagggttctc agcggcatcc ccatgcccga cttccgcgtc agcaaccggc aaaccgctat
    13321 cgccaccctg gttcaccccc aatacgtcaa cagtgtcaaa cacaacgtcg gctacggttc
    13381 catacaattc ggcaacgaca cccaaaaccc tgaagaacaa gcctatacct accgcctcgt
    13441 atcacgcaac ccgcacccgg actacgacta ccaccttccc cgcctcaaca aactggttac
    13501 cgaaatctca cctgccgcac tcagcagcgt acccttgctt ggaaacggcc agccaaaggc
    13561 caatgcctac ctcgataccg accgcttccc atactttgta cgactcggct caggcacgca
    13621 acaagtccgc aaagcagacg gcacgcgtac acgaaccgcc ccggcatacc aatacctgac
    13681 cggcggcacg ccgctgaaag tattggggtt ccaaaaccac ggcttactcg tcggcggcag
    13741 cctgaccgac caacccctta acacctacgc agtcgccgga gacagcggtt cccccctgtt
    13801 cgccttcgac aagcatgaaa accgctgggt gcttgcgggc gtactcggca cctacgccgg
    13861 cttcgacaac ttcttcaaca aatacatcgt cacgcaaccc gaattcatcc gttccaccat
    13921 ccgccaatac gaaacccggc tggatgtcgg gctgaccacc aacgaactca tatggcgcga
    13981 caacggtaat ggcggcagca ccctgcaagg gctcaacgaa cacatcaccc tgcccattgc
    14041 aaacccttcg cttgccccgc aaaacgacag caggcacatg ccgtctgcag atgccggcaa
    14101 aacgctcatc ctatccagca ggttcgacaa caaaacactg atgctggcag acaatatcaa
    14161 ccaaggcgca ggcgcattgc agttcgacag caacttcacc gtcgtcggta aaaaccacac
    14221 atggcaaggt gcaggcgtta tcgtagccga cggcaaacgc gtcttctggc aagtcagcaa
    14281 ccccaaaggc gaccggctct ccaaactggg cgcaggcacg cttatcgcca acggacaagg
    14341 catcaaccag ggcgacatca gcatcgggga aggcactgtc gtactcgccc aaaaagctgc
    14401 ttcagacggc agcaaacaag cattcaacca agtcggcatc accagcggca ggggcacggc
    14461 cgtcctcgcc gacagccagc aaatcaaacc cgaaaacctc tatttcggct tcaggggcgg
    14521 acggctcgac ctcaacggca acaaccttgc ctttacccat atccgccatg cggacggcgg
    14581 cgcgcaaatc gtcaatcaca accctgacca agccgcgaca ctgacgctga ccggcaaccc
    14641 catcctcagt cccgagcatg tcgagtgggt gcaatggggc aaccgtccgc aaggcaacgc
    14701 ggcggtttac gaatacatca acccgcaccg caaccgtcgg accgactact tcatactcaa
    14761 acccggcggc aacccgcgcg aatttttccc gttaaatatg gaaaactcaa caagctggca
    14821 atttatcggc aacaacaggc aacaggccgc cgaacaagtc gcccaagccg aaaatgcccg
    14881 ccccgaccta attaccttcg gcggatactt gggtgagaac gcgcaaacgg gcaaagccgc
    14941 accgagttac agcaaaacca atatcgaatg ggtgaatggt ccaaacaccc cacacaagcc
    15001 ctcgccctat acgaatacgc caacccgcac aggggcaggc gtaccgacga ttaccgcctc
    15061 aaagaaagcg gcaaccccgg tgcctacttc ccatggaatg cctacagcac ccaagactgg
    15121 gaatttctcg gacatgaccg gcgaagcagc catagaaaaa acccgccata tcgcaaatgc
    15181 cgccgtatac ggccggcccg aataccgtta caacggcgca ctcaacctgc actatcgtcc
    15241 caaacgcacc gacagcacgc tgttgctcaa cggcggcatg aaccttaacg gggaagtctt
    15301 gattgagggc ggcaatatga ttgtgtcagg caggcccgta ccccatgcct acgaccacca
    15361 ggccaaacgc gaacccgttc ttgaaaacga atggaccgac ggcagcttca aggctgcacg
    15421 gttcaccctg cgaaaccatg cccgactgac ggcagggcgc aataccgcgc atctggacgg
    15481 cgacataacc gcatacgatc tgtccggcat cgacctcggc tttacccaag gcaaaacacc
    15541 ggaatgctac cgctcctacc atagcggcag cacccactgc acacccaacg ccgttttaaa
    15601 agccgaaaac tatcgtgcac tacctgcaac gcaagtacgc ggcgacatta cccttaacga
    15661 ccgttcagag ctccgcctgg gcaaagcaca cctgtacggc agcatccgtg ccggcaaaga
    15721 caccgcagtc cgcatggaag cagacagcaa ctggacactt tcccagtcca gccacaccgg
    15781 cgcactgacg cttgacggcg cacaaattac cctgaacccc gatttcgcca ataatacaca
    15841 caacaaccgc ttcaacacac tgaccgtcaa cggcacactt gacgggttcg gcacattccg
    15901 attcctgacc ggcatcgtcc gaaaacaaaa tgcccccccc cccctcaaac tggaagggga
    15961 cagccgcggc gcattccaaa tccacgtcaa aaacaccgga caagaacctc aaacaaccga
    16021 atcgcttgca cttgtgagcc tcaatccgaa acacagccac caagcccgat tcaccctcca
    16081 aaacggctat gccgatttgg gtgcctaccg ctacatcctc cgcaaaaaca acaacggata
    16141 cagcctgtac aacccgctca aagaggccga acttcaaatt gaagccacgc gtgcggaaca
    16201 tgagcgcaac caacaggcat acaaccaatt acaggcaacc gacatcagca gacaggttca
    16261 acatgactct gacgcgacca ggcaggcact acaggcctgg cagaacagtc aaaccgaact
    16321 tgcccgcatc gacagccaag tccaatatct gtccgcccaa ttgaaacaga cagacccgct
    16381 gaccggcatt ctgacgcgtg cccaaaacct gtgtgccgca caaggataca gtgccgatat
    16441 ctgccgtcag gttgccaaag ccgccgacac gaacgacctg acactcttcg aaaccgaact
    16501 ggatacgtat atagaacgtg tagaaatggc cgaatccgaa cttgacaaag cacggcaagg
    16561 cggcgatgcg caagccgtcg aaacagcccg gcacgcctac ctgaacgcac tcaaccgtct
    16621 gtcccgacaa atccacagtt tgaaaaccgg cgttgccggc atccgtatgc cgaacctggc
    16681 cgaactgatc agccggtcgg ccaacaccgc cgtttccgaa caggccgcct acaataccgg
    16741 ccggcaacaa gcggaacgcc gcatcgaccg ccaccttacc gatccgcagc agcaaaacat
    16801 ctggctggaa accggtacgc aacaaaccga ctaccatagc ggcacacacc gtccctacca
    16861 acaaactacc aactatgcac atatcggcat ccaaaccggc atcaccgacc gtctcagtgt
    16921 cggtacgatt ttaaccgatg agcgcacaaa caaccgtttt gatgaaggcg tatccgcccg
    16981 aaaccgcagc aacggcgcac atctgttcgt caaaggggaa aacggcgcac tctttgccgc
    17041 ggcagattta ggctacagca acagccgtac ccgatttacc gattatgacg gggctgccgt
    17101 ccgccgccac gcatgggatg caggcatcaa caccggcatc aaaatcgata ccggcatcaa
    17161 cctcagaccc tatgccggca tccgtataaa ccgcagcaac ggcaaccggt acgtactcga
    17221 cggcgcagag ataaacagcc cggcgcaaat ccaaaccaca tggcatgccg gcatccgtct
    17281 cgataaaacc gtcgaactgg gtcaagccaa gctgaccccc gccttcagca gcgattacta
    17341 ccatacccgc caaaacagcg gttccgccct cagcgtcaac gaccgtacct tactgcagca
    17401 agccgcccac ggcacactgc ataccctgca aatcgacgcc ggatacaaag gctggaacgc
    17461 caaacttcat gccgcttacg gcaaagacag caacaccgcc cgccacaaac aggcaggaat
    17521 caaaataggc tacaactggt aacaagccga taaaaatgcc gtctggaacc cgcgtttcag
    17581 acggcatttg cgttaaaaat agtaaaccgt tccaaaaggg agtagaatag tgccgtttcc
    17641 aaccctgcgc ctgtaccgtc aggcttttat tatggacctt cccagttcgt ttttactgaa
    17701 caccccatcc gattcaaacc cgcaataacc atcccgccgg aatgtctccc cgcacacggc
    17761 gggcggagca tttatgagca tcgaacaaac ccctccgaac cttgaaaaca acggtatcga
    17821 aaacgatgta gaacgcgttt ccgccgattt cgaccgtgtc cactccctct gcgaaatcct
    17881 cgaacctgct tttgaacaaa tcgaaaacgg cacaccgctc gaagacgcgc cgctgcgcga
    17941 caagctgacc gagctgaccg tcctcttgag cgagctgcac cctgccgacg tggcggcggt
    18001 attggaatcg ctgccgccgc gcgaacgcaa tatcgtctgg attttggtca aaccggaaga
    18061 cgacggcgaa gtattgctgg aagtatccga cgcggtgcgc gaaacgctga tcgagtcgat
    18121 ggacaaggac gaattgttgg cggcagttga cgatttggac gcggacgaat tggcggaact
    18181 ggcagacgat ttgccgcacc aagtggttta cgaagcctta cagacgcgtg atgaggaaga
    18241 acgcgcccaa gtcaaggcgg caatgtccta cgaagacaac caagtcggtg cgattatgga
    18301 cttcgagttg gtcagcatcc gcgctgatgt cgcctgtgaa gtggtgctgc gctatctgcg
    18361 ccgcttcgac agcctgcccg accataccga caagattttt gtggtcgatg aaaacgacgt
    18421 gttgcagggc gtgctgccca tccgcaaact tttggtcgcc gatcccgaag atttggtgga
    18481 aaacgtgatg gcgaaagatg tcgtgcgttt ccgcgccgaa gacgacgtgg aagaagcggc
    18541 gcaggcgttt gaacgctacg acttggttac cgcgcccgtc gtcgatgaaa acaaaaagct
    18601 catcggcagg attaccgttg acgagatggt ggacgtaatc cgcgaagaat cggaagcgga
    18661 tatgctgaat atggcgggtt tgcaggaaga ggaagacctg ttcgcccccg tcttggattc
    18721 cgtcaaaaac cgctggatgt ggctcgccgt caacctctgc accgccttcc tcgccagccg
    18781 cgtcatcggc gcgtttgaag gcagcatcga aaaaatcgtc gcactcgccg cgctgatgcc
    18841 catcgtcgcc ggtatcggcg gcaactcggg caaccagacg attaccatga ttgtccgcgc
    18901 aatggcgatg gggcagttga cgggaatgca ggcggggcgc ttgttgaaaa aagaagtcgg
    18961 cgttgcccta gtcaacggca ttatctgggg aaccgtcatg ggcgcggttt catggctgct
    19021 ctacggcagc ctcggcatcg ggctggttat gattgccgcg atgacgctca acctcctgct
    19081 ggcggcaacc gtcggcgtat tgattcccgt ggtaatggaa aagttcggac gcgaccccgc
    19141 gctgggcagc tcggtgctga ttacggcggt aacggattcc ggcggcttcc tgattttctt
    19201 gggtctcgcc accctgttcc tgctttaaat gccgtccgga cccgcgcaaa aatgccgtct
    19261 gaagcggaag ctgcttcaga cggcatttga ctatttatcc ttgttgcaca agattattgg
    19321 acggtatgcc ggggcagccc tttggcaacg ccgaccacat cctccccgaa cagcgcgttg
    19381 acatcggttt cgtcaaacac atatttgctg tggcagaaat cgcaatcgac ttcgatgctg
    19441 ccttgttccg ccaccacgcc gccgacttct tccccgccca gcatcaacag catatcgctg
    19501 actttgccgc gcgaacaggt gcatgaaaat tcaaacgttt ccggctcgaa cacgcgcggc
    19561 ggcgtttcgt ggaacaagcg gtacaaaaca tgttgcgcat ccaattctgc caactcgccc
    19621 gccgtcagcg tgcgcgccag cgtactgacg tgttcccatg cctcttcatc caacacctct
    19681 tcaggcagac gctgcaccag cagaccgccc gccgcttcat cgcttgcaga caggacgatg
    19741 tgcgtatcaa gctgttcgga acgtttcata tagttcatca acatttgcgc gataccgtcg
    19801 ccttccaaag gcactacgcc ctgccagggt tcgccgtctt tgggctgcag cgtcagcacg
    19861 aatacgccgc cctcgcccaa aagatcgacc aaactttcat cttcaccgat ttctgccgcc
    19921 tcgtcccacc gcgcggttgc acggacggta cggtcggaag tcgcttctgc aaccagcatt
    19981 ttcagccgcc cctgcccctg aacctgcaca atcagcgtgc cttcgttttt aaggttgccc
    20041 gacagcagaa cgccggcagc cagtagctca cccaaggcgc ggcggatggc ggcgggatag
    20101 tttttctgtt ttacaatgtg ctgccacacg ttttccagac ggacgtgcag cccgcgcacg
    20161 ggcatatcgt cgaagataaa gcgggtacgc acatcggcgc ggtcgatggc ggtttgattc
    20221 atgattttct ctgactgatt gttcggatgg cggctatatg gttgcggtcg gcgcgaaaac
    20281 aagacggacg gcggatgcgc ttcccaaatt atcaataaat tatataaaaa tcaacatatt
    20341 aactcaatct aacaagccgt tttttgccaa acagccgttt ttttatatac aatcaacaag
    20401 atattttcga ctgatacagc ataacatcgc acggcggcac gatgcctcct gcgcggaaac
    20461 accgatatgg attctttttt caaaccggca gtttgggcgg ttttgtggct gatgtttgcc
    20521 gtccgccccg cccttgccga cgagttgacc aacctgctca gcagccgcga gcagattctc
    20581 agacagtttg ccgaagacga acagcccgtt ttacccatca accgagcccc cgcccggcgg
    20641 gcgggcaatg ccgacgaact catcggcagc gcgatggggc ttaacgaaca gcccgtttta
    20701 cccgtcaacc gagtccccgc ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg
    20761 atggggctta acgaacagcc cgttttaccc gtcaaccgag cccccgctcg gcgggcgggc
    20821 aatgccgacg aactcatcgg caacgcgatg ggacttttgg gtattgccta ccgctacggc
    20881 ggcacatcgg tttctaccgg ttttgactgc agcggcttca tgcagcacat ctttaaacgc
    20941 gccatgggca tcaacctgcc gcgcacgtcg gcagaacagg cgcggatggg tacgccggtt
    21001 gcccgaagcg aattgcagcc cggggatatg gtgtttttcc gcacgctcgg cggcagccgc
    21061 atttcccatg tcggacttta tatcggcaac aaccgcttca tccacgcgcc gcgcacgggg
    21121 aaaaatatcg aaatcaccag cctgagccac aaatattgga gcggcaaata cgcgttcgcc
    21181 cgccgggtca agaaaaacga cccgtcccgc tttctgaact gatttcccaa ggaatacgca
    21241 atgagtatgc ccgaaatgcc caaatggtac gacgatgacg gacagatcgt gtcctgtacc
    21301 gaaaaggtca aagtgatgtc cgaaaatatg gccgagctgt atcagacggc acaagacgcg
    21361 tttgaagacg cgctgctgat gggttgcggc gaacgtcagt tgcgcgatta cctgctcgcg
    21421 ctgattgaag gtttggaaaa tccctaccgc aaagtctgaa cacgccccgg ttgctgcggc
    21481 acggtttatc cgtgccgttt ttgcgtttgt gcgcggcttc ggcttttcag acggcatatt
    21541 tgacgttatg attaaacagt taacaagatt tatcacaacg ccgtcaaaag acagacacac
    21601 aacatgaaca tcatccgcgc gctcctcatc atcctcggct gcctcgccgc cggcgaaacc
    21661 gccgttttcc tagcaggcat caaactgccc ggcagcatcg tcggcatggg cgtgctgttt
    21721 gcgcttttgc aggcgggttg ggtcaaaacg tcttggctgc aacagcttac cgacgcgctg
    21781 atggcgaatc tgacgctgtt tctcgtgccg ccctgcgtgg cggttatcag ctatttggat
    21841 ttgattgccg acgattggtt ttcgatactg gtttccgcct ccgccagcac tttgtgcgta
    21901 ctgctggtta caggcaaggt tcaccggtgg atacggggta ttatccgatg aacgaaatcc
    21961 tcaggcagcc cagcgttctg cttttcctca cgcttgccgt gtacgcgatt gcgattatcg
    22021 tgcgcacgcg cacgggtaat atcttctgca accccgtact cgtcagcact atcgtgctga
    22081 ttgcctacct gaaaatcctc ggtatcgatt atgcggtgta ccacaacgcc gcgcaattcc
    22141 ttgatttttg gctaaaaccc gccgtcgtcg tgcttgccgt gccgctctac caaaaccgcc
    22201 gtaaaatctt caaccagtgg ctgcccgtca tcgtttcaca gcttgcgggc agcgttacgg
    22261 gcattgttac ggggatgtat tttgccaaat ggctgggcgc ggaacgcgaa gtcgtcctct
    22321 cgctcgcgtc caaatctgtt accaacccca tcgccattga aatcacccgc tccatcggcg
    22381 gcattcccgc cattaccgcc gccaccgtca tcattgccgg tctggtcgga cagattgccg
    22441 gttacaaaat gctgaagaac acagtcgtca tgccctcgtc cgtgggtatg tcgctcggca
    22501 cggcttcgca cgcgatgggg attgccgcct cgctcgaacg cagccgccgc atggcggcat
    22561 acgcgggact ggggctgacg ttcaacggcg tactgaccgc gctgattgcg ccgctgctca
    22621 tccccgtttt gggattttga acccgtttca gacggcattt cagcccatgc tgtctgaacg
    22681 ccgacacact cgcaaggaga accgttatgg ctgtcaacct gactgaaaaa accgccgaac
    22741 aactgcccga catcgacggc attgccctct acaccgccca agcaggcgtg aagaagcccg
    22801 ggcataccga cctgacactg attgccgtag ccgccggcag caccgtcggt gcagtcttca
    22861 cgaccaaccg tttctgtgcc gcgcccgtcc acatcgccaa atcgcacctt ttcgacgaag
    22921 acggcgtgcg cgccctcatc atcaacacgg gcaacgccaa cgcgggtacg ggcgcacagg
    22981 gcagaatcga tgctttggca gtgtgtgccg ccgccgcccg gcaaatcggc tgcaaaccga
    23041 accaagtgct gcccttctcc accggcgtga ttctcgaacc gctgcccgca gacaaaatca
    23101 tcgccgcgct gcccaaaatg cagcctgcct tctggaacga agcggcacgc gccatcatga
    23161 ccaccgacac cgtgcccaaa gccgcctcgc gcgaaggcaa ggtcggcgac aaacacaccg
    23221 tccgcgccac gggcatcgcc aaaggctcgg gcatgattca tcccaatatg gcgaccatgc
    23281 tcggtttcat cgccaccgat gccaaagttt cccaacccgt cctccaactg atgacgcagg
    23341 aaatcgccga cgaaaccttc aacaccatca ccgttgacgg cgacaccagc accaacgaca
    23401 gcttcgtcat catcgccacc ggcaaaaaca gccaaagcga aatcgacaac atcgccgacc
    23461 cgcgttacgc ccaactcaaa gaattgttgt gcagcctcgc gctcgaactc gcccaagcca
    23521 tcgtccgcga cggcgaaggt gcgaccaagt tcatcaccgt ccgcgtcgaa aacgccaaaa
    23581 cccgcgacga agcccgccaa gccgcctacg ccgtggcacg ttcgccgctg gtcaaaaccg
    23641 cctttttcgc ctccgacccc aacctcggca ggctgctcgc cgccatcggt tatgccggcg
    23701 ttgccgacct cgataccgac ctcgtggaaa tgtatctcga cgatattttg gttgccgaac
    23761 acggtggacg cgccgcaagc tacaccgaag cacaagggca ggcggtgatg tcgaaggccg
    23821 aaatcaccgt ccgcatcaag ctgcatcgcg gacaagccgc cgccaccgtc tatacctgcg
    23881 acctgtcgca cggatacgtt tccatcaacg ccgactaccg ttcctgactc gacacggctt
    23941 cagacggcat acataaaatg ccgtctgaac cgccggacaa cataccatga cctccacatt
    24001 cccccgccgc ctcgcccgca aaatccgcca aacccgccgc ctgtcgctca aaagcatcgc
    24061 ctttctgttc cttttggcag gttcggcact cgtcgccctg accgcgctgt tttttgccca
    24121 tcttgccgat tttgcgctgg aactgaacgc caaactggtt caacaatacc cgtggttcgc
    24181 gtgggtcgcg cttcctttgg gtttaccgct tattgcgtgg ctcacacgca aattcgcccc
    24241 cttcaccgcc ggcagcggca tcccgcaggt catcgcctca ctgtcgctgc cctacggcgc
    24301 acagaaaacg cggctgatcc gcctcgggca gacgctgctg aagattccgc taaccttttt
    24361 gggtatgctg ttcggcgcgt ccatcggacg cgaaggtccg tccgtgcagg tcggcgcgac
    24421 agtgatgggc gcgtggggcg cgtggtgcaa gaaacacggc ttggcattca aagggatgca
    24481 ggaaaacgat ttgatggcgg cgggcgcggc gggcggtttg gcagccgcgt tcaacgcgcc
    24541 gctggcgggc gtgattttcg ccattgagga actcgggcgc ggcatcatgt tgcgctggga
    24601 gaggcaaatt cttttgggcg tgctcgcctc cggtttcata caggtcgcca ttcagggcaa
    24661 caacccgtat ttttccggct tcaacggcgg cgtattggaa catatctttc tgtgggtcgc
    24721 actgtccggc ctggtttgcg gcgcggcggg cggcctgttc ggacgtttgc tctatcgcgg
    24781 tgcggcggcg tttgcaccgc gcaagatacg cggcttcatc cgcaaccgtc cgctgctgct
    24841 ggcggcactg atggggctgc tgctcgccct gctcggcacg ttctaccaag gcaaaaccta
    24901 cggcactggc taccacgaag ccgcccaagc cctgcacggc atctacgaag cccccttcgg
    24961 actcgccgcc gccaaatggc tcgccaccgt attcagctat tgggcaggca ttccgggcgg
    25021 cattttcact ccctcgctga ccataggcgc ggttttgggc gagcatatcg ccgccatcgc
    25081 cgacatatcg cagggtgcaa acatcatcgt cctcatctgc atggcggcat ttctggcggg
    25141 cgcgacacaa tccccgatta cttccgccgt cgtcgtcatg gaaatgacgg gcggacaaag
    25201 cctgctgttt tggatgctaa ttgcctgcat tttcgcctcg caggtttcgc gccagttttc
    25261 gccgcgtccg ttctaccacg catcgggaat gcgcttccgc cagcgcgtgc ttcaagaaac
    25321 cgccgcccaa accggcaatg cgcccgcaag accgcaaaca gcaaacagca aaacgggaat
    25381 gccgtctgaa aattaaaacg cccccgatca aacgccggca gccgccttga tttgaatacc
    25441 gttccgccgc cgcttgaaat ttcagcaaca atgccgtctg aacgacagaa tgcggttttc
    25501 agacggcatt tccccatccc gatattgcct aaacaaaacc gaagcgtttg ctataattct
    25561 attttttacc gcacacgcac caatcatgtt tcccgatttc tcccaaaccc tctccaaaga
    25621 ccgccacttc ctacgttccg ccttcaaaaa tcccaacaaa tacggcggct tgtccaaagt
    25681 cgaagaaaaa taccaaaaat cgcacgaaat ctttttgaag cgtttggctg ccttgcccaa
    25741 acccgaattc gataacaccc tgcccgttca cgagaagctc gacgaaatca aaaaagccat
    25801 tgccgagaat caggtaacga ttatttgcgg cgaaaccggt tcgggcaaga ccactcagtt
    25861 gcccaagatt tgtctggaac tcgggcgcgg ggcggcgggt ttgatcgggc atacccagcc
    25921 gcgccgtttg gcggcgcgtt ccgtagcaga gcggattgcc gaagagctga aatccgaaat
    25981 cggcagcgcg gtcggctata aagtgcgctt caccgaccac acctcgcgcg atgcctgcgt
    26041 caagctgatg accgacggca tcctgctggc ggaaacccag accgaccgtt atctcgccgc
    26101 ctacgacacg attatcatcg acgaagcgca cgagcgcagc ctgaacatcg acttcctttt
    26161 gggctatttg aaacaactcc tgccgcgccg ccccgatttg aaagtcatca tcacctcggc
    26221 aacgatagac gcagaacgct tttcccaaca cttcaacggc gcgcccgttt tagaagtgag
    26281 cgggcgcacc tatcccgtcg aaatcctcta cagcccgctg accggcaaag acgaagacga
    26341 cgcagaagtc gagctgaccg acgcgattgt cgacgcggcg gacgaattgg cacgctacgg
    26401 cgaaggcgat attttggtgt tcctgccggg cgaacgcgaa atccgcgaag ccgccgaagc
    26461 cctgcgcaaa tccacgctac gccgcaacga cgaaatcctg cccctgttcg cacgcctgtc
    26521 gcacgccgag cagcacaaaa tctttcaccc cacaggcgcg aaacgccgca ttgtgttagc
    26581 aaccaacgtc gccgaaacct cgctcaccgt gccgggcatc aaatacgtca tcgacaccgg
    26641 cctcgcacgc gtcaaacgct attccgcacg ggcgaaagtg gagcagctcc atgtcgaaaa
    26701 aatctcccaa gccgccgccc gccaacgctc cggccgctgc ggccgcgtct ccgcaggcgt
    26761 atgtatccga ttgttttcag aagaaaattt caacaaccgc cccgaattta ccgaccccga
    26821 aatcgcccgc agcaacctcg ccgccgtcat cctgcgcatg gcatcgctga aactcggcga
    26881 cgtagcggca ttcccatttt tagaaatgcc cgattcgagg tatattaatg atgggtttca
    26941 ggtgttgctg gaattagggg cagtggaggc cgcctgaaaa caagtaggat atacacttct
    27001 acctgacaaa gaccaacaaa cgataaaact acggcacatc tagatgccca acctacaaca
    27061 ttgcacacgg taaaaacatg aaacacttac tcatcgattt tgaaaacgtc cagccgcaaa
    27121 acttagacaa attactaacc gaaaataccc atatttggct atttataggt gtattacaca
    27181 aaatgttacc tattagtctg gtgcaatccc tactacgttt cggcgaacgt gtccatcttg
    27241 tccagttaca aaaaacaggg aaaaacgcat tggattttta cctgtcctat tacctcggac
    27301 aaattaccgc cacagacccc aatgcccaaa tcggcatact ctcgcgtgat ggaggatacg
    27361 atgttctggt cgaacatatt ttgaaaaacc accaggcgaa gggtatcgtg cgcctagcca
    27421 atatagatga agtacaacat cagaaaatta ctaccgaacc gccgtcagca ttgcgggaaa
    27481 acactcctca gcctgaaacc accctcaaac cacagcaacc attaacttcc tatttccaag
    27541 cagccctaac tgcactgcgc cgccccgacg ctttccgccc ctgccgcctg cataacctgc
    27601 gacaaaatct gcgtaagcat attttgagtg atttgtttaa agaaaaaacc gatgaagaat
    27661 gcgaaataac cactgctaac gttatcaata aactcaaagc acaaaacttc atcagcattg
    27721 atgaacagga aaccgtttcc taccatctca gtgataatga tttgttacaa agaatccaac
    27781 gccatatttt aagccaacgt cccaaaacct acgctgattt tcaagccgtc gtgcaaaacc
    27841 gagcagatgc acttcactta acagtcggta ccaacgacat tcaatccttt gcgcgacatt
    27901 tgcgcgacca aaacctgatc cgccaaaaca atgggaaaat tgaatatgca ccgtttactg
    27961 aacctaaacc acagccaacg cccaagcagc ctaaaaaaac cgcatgggaa cctgatgaaa
    28021 ttatttggaa aaaagtgatt gccgcgttat cgttaaagaa ccgtcctaat aaaaccaaaa
    28081 ctttacgcaa tacaatccag gcactcacaa aatccaatgc acaagaaact gacaaactgc
    28141 tacaacattt acaagatacc caagtcctac gtattgacgg tactaaaatt gtgtatatca
    28201 agtaggatgg gcatttatgc ctgacctaca aaaccaaaaa caacctacca cccttaatca
    28261 actccacaaa cccttttcag acaacctcgt tttttgaaaa acaatctgta aacagatagc
    28321 tgctgaagaa taccgttgcc gagccccaaa acccgtcctg caacttttat tgtgaactgc
    28381 ccattatgaa aaaatccctt ttcgttctct ttctgtactc atccctactt accgccagcg
    28441 aaatcgccta tcgctttgta ttcggaattg aaaccttacc ggctgcaaaa atggcagaaa
    28501 cgtttgcgct gacatttgtg attgctgcgc tgtatctgtt ttcgcgttat aaaacttcgc
    28561 ggctgctgat tgcgctgttt ttcgcgttca gcattattgc caacaatgta cattatgcgg
    28621 tttatcaaag ttggatgacg ggtattaact attggctgat gctgaaagag gttaccgaag
    28681 tcggcagtgc gggcgcgtcg atgttggata agttgtggct gcctgcgttg tggggcgtgt
    28741 tggaagtcat gttgttttgc agccttgcca agttccgccg taagacgcat ttttctgccg
    28801 atatactgtt tgccttccta atgctgatga ttttcgtgcg ttcgttcgac acgaaacaag
    28861 agcacggtat ttcgcccaaa ccgacataca gccgcatcaa agccaattat ttcagcttcg
    28921 gttattttgt cggacgcgtg ttgccgtatc agttgtttga tttaagcagg attcccgcct
    28981 ttaagcagcc tgctccaagc aaaatcgggc agggcagtgt tcaaaatatc gtcctgatta
    29041 tgggcgaaag cgaaagcgcg gcgcatttga agctgtttgg ctacggacgc gaaacttcgc
    29101 cgtttttaac ccggctgtcg caagccgatt ttaagccgat tgtgaaacaa agttattccg
    29161 caggctttat gacggcagtg tccctgccca gttttttcaa tgcgataccg cacgccaacg
    29221 gcttggaaca aatcagcggc ggcgatacca atatgttccg cctcgccaaa gagcagggct
    29281 atgagacgta tttttacagc gcgcaggcgg aaaacgagat ggcgattttg aacttaatcg
    29341 gtaagaaatg gatagaccat ctgattcagc cgacgcaact tggctacggc aacggcgaca
    29401 atatgcccga tgagaagctg ctgccgttgt tcgacaaaat caatttgcag cagggcaggc
    29461 attttatcgt gttgcaccaa cgcggttcgc acgccccata cggcgcattg ttgcagcctc
    29521 aagataaagt attcggcgaa gccaatattg tggataagta cgacaacacc atccataaaa
    29581 ccgaccaaat gattcaaacc gtattcgagc agctgcaaaa gcagcctgac ggcaactggc
    29641 tgtttgccta tacctctgat catggccagt atgttcgcca agatatctac aatcaaggca
    29701 cggtgcagcc cgacagctat ctcgtgccgc tagtgttgta cagcccggat aaggccgtgc
    29761 aacaggctgc caaccaggct tttgcgcctt gcgagattgc cttccatcag cagctttcaa
    29821 cgttcctgat tcacacgttg ggctacgata tgccggtttc aggttgtcgc gaaggctcgg
    29881 taacgggcaa cctgattacg ggtgatgcag gcagcttgag cattcgcgat ggcaaggcgg
    29941 aatatgttta tccgcaataa gcggcgtgaa aaccaataaa gacaaattta gatgatgtcg
    30001 gggaagatgc ccgaccgaca agactatgca aaatatgaaa aaccaaatac gcggatcagg
    30061 catggatgcc cgatccaatc cggccaatgt ttcagacggc ctgcaaaaca gttcgggcca
    30121 tatcggtacc aacacgcgct accgtctgac caaactcggc gaacaaatgg cgcgcctgcc
    30181 catcgacccg aaaatcgcgc gcattttgtt ggcggcgaag aagcacgact gcatggcgga
    30241 aatattggtg attgcgtccg cgctattgat tcaagacccg cgcgagcggc cgctggaggc
    30301 acgcgatgcc gccgccaagg cgcatgaacg ctttaccgac aagcaatccg atttccttgc
    30361 ctacctgaat atttgggaca gtttccagcg cgagcgcgac aaaggtttgt ccaacaagca
    30421 gctggtgcaa tggtgccgcc aatatttcct gtcgcacctg cgtatgcgcg aatggcgcga
    30481 gctgcaccac cagcttgccc aaaccgcgat tgaaatgggt ttgaccacca aggaagccgc
    30541 tttcagacga cctcccgaaa tcaggcagct cacgtcgtct gaaaaccaag gcgaccaaga
    30601 cctatccgcc aaactcaagc aaaaacaact ggataaaaag caacaccgcg cccaaatccg
    30661 cgccgccaaa gaagcgggct acgaacaaat ccaccgcgcc ctgctcactg gccttatcgc
    30721 caacgtcggc atgaaatcgc ccgacggcaa cgactacacc ggcgcgcgcg gcagccgctt
    30781 ccaccttttc cccgcctccg ccctgttcaa agccaagccc aaatgggtga tggcggcaga
    30841 attggttgaa accacgcgcc tttacgcgcg cgacgtcgcc gttatccagc ccgaatggat
    30901 agagcaggaa gcgccgcacc tcgtccgcta tcattatttc gagccgcatt gggaacaaaa
    30961 acgcggcgaa gtcgtcgcca gcgaacgcgt gacgctttac ggcctgaccg tattgccgcg
    31021 ccgccccgtg tcttacggca aagttgcccc cgaagaagcg cgcgaaatct ttatccgcag
    31081 cgcgttggtg gcgcaggaat gcgatttgaa agcggatttt tttgtccaca acaaaaagct
    31141 gattaaagaa attaccgaac tcgaacacaa atcgcgcaag caagacgtgc tggtcgatga
    31201 cgaagccctg tttgcgtttt ataacgaacg actgcccgaa atggcttgga aagacgcgca
    31261 aggcagcgtt tggggaagtg aagattccgt acggattatt gaatctgaca aagccgagag
    31321 gtcgtctgaa aatgagcgca acgagtttcg taaaaacaag cgtaatgggt ctcgccaaaa
    31381 tgaaaatcac ggcaacaccg taggttgggt tgaaaaccca acatcagccg caactgcaaa
    31441 aactgttggg tttgacaacc caacctacgc tactcagcaa cccaccccct cccccgagag
    31501 ggagggtcgg ggagagggca aaacagttgc cgcacaaacc aacttttccg caaccgcagc
    31561 aaaccctctc ccgcaggaga gggaacagag tgcctcagct tcaacgtttt cagacgacct
    31621 gcgtcctgca aatctgcagc aaaccgcccc ctcccccgtg ggggagggct ggggagaggg
    31681 caaaacagtt gccacacaaa ccaacttttc cgcaacctca acaaaccctc tcccgcagga
    31741 gagggaacag agtgcctcag cttcaacgtt ttcagacgac ctgcgtcctg caaatctgca
    31801 gcaaccctct ccctcccccg tgggggaggg ctggggagag ggcaaaacag ttgccaccca
    31861 aaccaacttc tccgcaacca cagcaaaccc tctccctaac cctctcccac aggaggggga
    31921 acagagtgcc gcagcttcag cggtttcaaa cgaccctcaa ccccaaaagc agccagcacc
    31981 ccaaaaagac cgtctgaaac ccctccccct cgccgacatc cgcaccttcc aagcctggct
    32041 caaaaccgcc gaacgcgaga acccgcgcct gctgttcctc agccgcgacg atttgatgca
    32101 acacgccgcc gcacacatta ccgaagaaca attccccaaa ttctggcaaa ccgcagacgg
    32161 caagttcgaa ctcagctacc gcttcgagcc gcaccacccg ctcgacggcg ttaccctcac
    32221 cctaccgctg accgtcctca accgcctgca cgcgccgtcg ctcgaatggc tggtgcccgg
    32281 tatgttgcgc gaaaaaatcc agctgctgat taaagctctg ccgaagcaaa tccgccgcat
    32341 ttgcgtgccc gtacccgatt tcatcaccca atttttaagc caaaaccctg atcgcaacgc
    32401 ccccatcctg ccccaacttg cccaagccat cgccaaaacc gcaggcgaca tccgcatact
    32461 cgagcaaatc aaccaagacg aatgggccgc gttcaggctg cccgaacact gctatttcaa
    32521 cctccgcatt atcgacgacg gcggacaaga gcttgccggc ggccgcaaac tgcatgaatt
    32581 gcaacaacag ctcggccaag ccgccgccgt taccttccgc gacaacaccc aagaattcga
    32641 gcgcgacaac gtgaccacat gggacattgg caccctgccc gaatccatca aattcgcacg
    32701 cggcaaacaa cagctcaccg gctacctcgg tctgcaaaaa gaaaaagacg gccgcatcgc
    32761 cctgcgcctg tgtgacacca ccgaagctgc cgaacaggca caccgtcaag gcgtcatcga
    32821 attgatgaag ctgcaattaa aagagcaggt aaaagacctg aacaaaggca tccaaggctt
    32881 tacccaagcc gccatgctgc tcaaacacat caacgccgac accctgcgcg acgacctcac
    32941 ccaagccgtt tgtgaccgtg cctttatcgg cgaagacgag ctgccgcgca acgaaaaagc
    33001 cttcaaagag caaatcaaac gcgcccgcag ccgcctgccc gccgtcaaag aagccctcag
    33061 ccgctacttg caggaaaccg ccgccgccta cgctgaactg aatagcaaac tcggcaaaca
    33121 tccattgacc cacctcctaa gactacgcct gcaaaccctg ctcgccgccg gctttgccag
    33181 ccataccccg tgggtacaat ggccgcgcct ccccatctac ctcaaagcca tgaccctgcg
    33241 cctcgaaaaa tacagcagca accccgcacg cgacgcatcc cgcgaagccg atatacagga
    33301 attggaacaa atgtggcagg aaaaaaacga cggtttggtg aaacaaggac tacccgtttc
    33361 agacgacctc accgcgttta aatggatgat tgaagaattg agagtgtcgc tgttcgcgca
    33421 ggagttgaag acgccgtatc cggtgtcggt gaagcggttg ttgaagatgt gggaggatct
    33481 taattaaagg aagatgccgt ctgatttttt cagacggcat cttttatttc cctgaccaac
    33541 cgatttaatc gctccctacc aatagcttta ttttttaatt cgcactccca aacgacaatc
    33601 actttgaaac cgatcttttc cagctcggtt tcatttttaa tatcacgttc gcaatttttt
    33661 gtgatttttt ccaaccaaaa atccgtattg cttttcggga tatgtccttt attacaagaa
    33721 tgcccgtacc aaaaacagcc gtgtataaac acgactgttt tatatttcgg caaaacgata
    33781 tccggtttgc ctgcatagcg tttatcgttt ttccgataac gaaaaccttg ggagaaaaga
    33841 aatttcctta ccaatatttc cggtttggtt tctttgctat ggatttttga catcacgaaa
    33901 gaacgtttgg atgtagtgaa aatatcggtc ataaattact tggaagatac caccttcgat
    33961 cgtttttttg tctgttccac ttttcttaac aaatacgaaa ccaagcttat taaatccaaa
    34021 gcatccttat cattaaaaat actaggagat aaatttgctc ttaactcatg ttgtacagga
    34081 tttctaaatc cagtccatgt tccgcatgca agctgttcaa gaccatcttc taaattttgt
    34141 tccgccttcg ttttgttatt cgttaataga agtatagaat ttttactccc gaaagcttgt
    34201 tcaatcaatt ctcttccgtc tatgctatga agacctgttt gcttctgaac cttatcttcg
    34261 aaaactttaa ctgcctcgat tgcagcactt aaataatcct tcttttcatt gtaaaaatca
    34321 ttacaggcag tatgcaaatc ctgatgcaaa tgccgccaat gatagtaagg atattctgga
    34381 ataatactat gtaaggcaga gattacttcc gcttgctctt tatttgtaag ctccgaatct
    34441 tccaaacgat tcaaaataac ttctactggt tccctttcct tttctggcaa tttgtttttc
    34501 caatcttcga tatcaactcc gctttcgcct ttcacttcgt catctttctc tttttttctt
    34561 ctttttctcc aatcttgccc gatggctcgg acgatctccc caaggaaaac acgtaatttt
    34621 gaggtttttg aatgcttcca gtctaatgat gtcctgtcgg tagcaattaa atcttcatcg
    34681 tcattatcta taaagtccac attcagccat ccagtcaaat atgaatagaa atggctggat
    34741 tcgctatcag tgaaaaactc gggcatattt accattctgc cgttggcaaa caaggtaata
    34801 cctttcatat tattctttaa aggtttttcc gttgtaatga acttaccttg tattaatccg
    34861 ctgtattcat actgttcgaa ttttgaagat aacgatgaaa tattggttgc tagatcctgg
    34921 tattcccatt cgaattgtgg ggtaacaaga tcatatttta gatttccatc aatgatttta
    34981 ggttcagagc cgttcaagct tactttgatt ttaaaatcct gagccggaaa atcaaacaag
    35041 cgggaaagat gctctacata attatctaac ggatatcctt gctttttcgt cagttcggtt
    35101 aaagttatgg ttgtcccgct ttcgatattg gattcaacag attcttttcg aaactccggt
    35161 tgataaatac ccttgcttct tcgaatctct gcataatcca aagtaaaagt aaccctttcg
    35221 tttccctgga tagtagaaat ttcaattttg ttgccaagcc cgaataatgc caatttacca
    35281 aggccttttt tacccgttgg aattcttccg cacggggaag cttgtttttc ttcccttctg
    35341 ttccgaccga ttctcaaata aaaatcattg atttcatcga agctcattcc tataccatta
    35401 tctttgataa cgattttatg ctccggttta tcgaataacc taacttccac ttccgtagca
    35461 catgcatcgt aggcattcgc tatcaactcg gcaatcgcag gaacagtgtg cgaatacatc
    35521 tttacaccca aatgttcgat tacggttggc tcgaaactca ttttcaattt ttcttcagac
    35581 atgtttttct ccttttctaa cattcgatat tatcaacaat tgccctgcct attttttccg
    35641 catacatcgg aggaacggca ttaccaatca aacgggctat cttgtccctg ctgcccgctt
    35701 taaagacata gttacgaggg aacgattgca atgttgcacc ttcgcgcagt gataacgccc
    35761 tatcttcttc aggatgggca aacctcccgt tggaaatact aaagaatttg gtcgttatgg
    35821 taggtgcggg cttatcccac catagcctac caaacgtgtc tttaaaacta ttgtcttttc
    35881 cgataaaaca ttccagctgt aattcgggaa tatgcgcaaa agccaaacgg tttcctccgt
    35941 ttttaggtat caaagccaat cgtttcaagt tgatatcaga taatcccgca cagctatgca
    36001 taaaatccgt ttcgtcttgg tgtcctgcaa taattttggg aaagccattt tccattccta
    36061 aaacatcgcg taccgtaagc cgtttgcccg aatacttggc tggttccagc ttctcttcgg
    36121 ttattctgtt tgcaatcaaa gtaaacctct tacgactttg gggaattccg taatcagccg
    36181 tattgtgaat accgaaatgg acggtaaatc ctttttcttc taataatttt atgaaattat
    36241 caagcccgct ttctttcatc ctgctcagaa ttcccggtac gttctctaca acaacatatc
    36301 cgggattaaa atattctaca aaccgctgaa actccaaaag taaactcttg gatttttccg
    36361 attttctttt gtctgtttga ataacactcc aatattgaca aggactacaa cctataagaa
    36421 tcaaatcatc atcattcttt tttaacccga gttctttttc caaggtttca ggctgcaatt
    36481 caaaaacatc agcctgtata aatttggcat ttatattggc ttcatatgtt tctttgcagc
    36541 tgggttcgta atctatcccg gctaaaacct gaataccggc ggattgcatt ccatagctca
    36601 tgccacctcc gccacagaaa aaatcaaccg cctttaactt actgtttttc atttctattt
    36661 aaataattag actagtgaga actaacttta tataacatat aatttattgc ttatcaaaaa
    36721 taatttgaga ttttattaaa attcatgcat ctaaataagc agaacttaat ttcctccaaa
    36781 cgagcggatg tcttcaaatg cacacttcac agaaaggtcg tctgaaaccc attttcagac
    36841 gacctttttc cacttcccaa aacaagaatt cccaccccct ccccaaacgt cgcccgaacc
    36901 agtaaacttc tcatatttca acaactcctt ggaagcaaac catgtctggt atctacctac
    36961 cccgcctatt cccgccccat atcgccgaac gcggcctgtt gtattttcag cagggcaagg
    37021 ttctcgatgt ccgaaaaact tccgccgggc attatcgggc ggaggtgtgc ggttcggaaa
    37081 actattgggt atagttgaag ctggatagtg atttgtatat taaagacgaa ggctgcaatt
    37141 gtccttatat ctaagagtgc aaacatacct taaattacta tattgcatag gcaaaataca
    37201 agcctataac gaattggaaa caaaatgccg tctgaaaaca tcttcagacg gcattataaa
    37261 atctgttcac cttttcagat gagtaatgta cacccttata caatttttgc tactatgccc
    37321 cataaatcca cggctaaaga tatccttatt atgtcctatg atttatcgaa acgacttgta
    37381 atcggcttag catcaagtgc cctattcgac ttatccgaat cggataatat atttagaatg
    37441 gaaggggcag aaacctatag gcaatatcag agagaaaaac aaaaccatcc cctaaaaaag
    37501 gcgttgtctt tccatttatt aaaaaacttc tgtcaatcaa tgaaataaac ccaaacgacc
    37561 caacgattgg gtttattctt ttatccagaa acaatccaga gacagattac gagtcataac
    37621 tataggctta atattacacg attttcattc catcaaggcg gaaaaccgca caaatactga
    37681 aacactatcg atcgatttgt aaacaagcct acttaagtaa cttgcagtcc ttatcatttc
    37741 ctttaaaata atccagcccg tcactacacg aactggcgga cttcttgcaa ataaaggtta
    37801 ctagattttc attcatctta ataataaaag gatttttatc tttatctatg gctaccgcct
    37861 tcaacatgaa tttactgtct aaggccccgc gcgcgattcc attcaaacgg atacaaaagc
    37921 cttctgcctc tttaatcggc aaacttggcc atttggtaga tgtttgttta aatctcccat
    37981 tctgcagata aaacttttcc ataaaatgtg cattttctaa caaggctgcc cgcactgtat
    38041 ttatctttgc tttctcaaca taattgcgat agctcggata aacaattaaa gcaagtacag
    38101 acaatatcaa gaccactgat attaattcaa ccagcgtaaa cccccgatta tcagtcatta
    38161 ctttacttcc aataagaaca gattattcaa catatttctt tgaacagact tactatccca
    38221 ttcagcagta tgcatatttc ccactctatt ttttatccgc gggcagcact ggtttggc
//
LOCUS       DAXIEB010000005        33907 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000005 DAXIEB010000000
VERSION     DAXIEB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.005"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(58..1368)
                     /locus_tag="ACFOA3_000216"
     CDS             complement(58..1368)
                     /locus_tag="ACFOA3_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6897294.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="ACFOA3_000217"
     CDS             complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="ACFOA3_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6897295.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="ACFOA3_000218"
     CDS             complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="ACFOA3_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6897296.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4134..4862)
                     /locus_tag="ACFOA3_000219"
     CDS             complement(4134..4862)
                     /locus_tag="ACFOA3_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6897297.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4852..5766)
                     /locus_tag="ACFOA3_000220"
     CDS             complement(4852..5766)
                     /locus_tag="ACFOA3_000220"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6897298.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGVVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            complement(5880..7289)
                     /gene="murC"
                     /locus_tag="ACFOA3_000221"
     CDS             complement(5880..7289)
                     /gene="murC"
                     /locus_tag="ACFOA3_000221"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6897299.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7444..8511)
                     /gene="murG"
                     /locus_tag="ACFOA3_000222"
     CDS             complement(7444..8511)
                     /gene="murG"
                     /locus_tag="ACFOA3_000222"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6897300.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8515..9828)
                     /locus_tag="ACFOA3_000223"
     CDS             complement(8515..9828)
                     /locus_tag="ACFOA3_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6897301.1"
                     /translation="MTGGFLKISEVLVKVGDGVHTLLLDRQIVRDGRKFDAPLLWMVV
                     LMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRL
                     VPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRRE
                     EVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLI
                     MVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAF
                     LDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFG
                     FFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGA
                     LPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9965..11302)
                     /gene="murD"
                     /locus_tag="ACFOA3_000224"
     CDS             complement(9965..11302)
                     /gene="murD"
                     /locus_tag="ACFOA3_000224"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6897302.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11390..11662)
                     /locus_tag="ACFOA3_000225"
     CDS             complement(11390..11662)
                     /locus_tag="ACFOA3_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6897303.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGLAQAQ
                     YSLGAMYYKGRGVRQDLVLAQEWLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(11777..12859)
                     /gene="mraY"
                     /locus_tag="ACFOA3_000226"
     CDS             complement(11777..12859)
                     /gene="mraY"
                     /locus_tag="ACFOA3_000226"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6897304.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13106..13282)
                     /locus_tag="ACFOA3_000227"
     CDS             complement(13106..13282)
                     /locus_tag="ACFOA3_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897305.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(13285..14643)
                     /gene="murF"
                     /locus_tag="ACFOA3_000228"
     CDS             complement(13285..14643)
                     /gene="murF"
                     /locus_tag="ACFOA3_000228"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6897306.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALANNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGVDSGDVHAENIVLKPLSCEFDLVCGNERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLDDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLVRMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEEFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14768..16003)
                     /locus_tag="ACFOA3_000229"
     CDS             complement(14768..16003)
                     /locus_tag="ACFOA3_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6897307.1"
                     /translation="MYRGGVFADVKNYKRRSKIWLTILLTLILSCAVMDKIASDKDLR
                     EPDAGLLLNIFDLYYDLAFAPAQYAAKRAHILEAAKKVSTWHIRHVAPKYKNYVVVIG
                     ESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAHATNLSLPQTLGLPGEPNNNIV
                     SLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPWFTQRGDYGKSAGLSDRLLLPA
                     FKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARRFQYQTEKISCYVSTIAQTDKF
                     LEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLTHGAWKRQSYGVPLVKISSDDT
                     RREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFWGNVPDVQGEGNNLAFIDGLPD
                     DPAPWYAGKGKSTKNTSKK"
     gene            complement(15978..16415)
                     /locus_tag="ACFOA3_000230"
     CDS             complement(15978..16415)
                     /locus_tag="ACFOA3_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897308.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGGIC"
     gene            complement(16438..17916)
                     /locus_tag="ACFOA3_000231"
     CDS             complement(16438..17916)
                     /locus_tag="ACFOA3_000231"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6897309.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTLWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(17941..19686)
                     /locus_tag="ACFOA3_000232"
     CDS             complement(17941..19686)
                     /locus_tag="ACFOA3_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6897310.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPIDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(19747..20010)
                     /gene="ftsL"
                     /locus_tag="ACFOA3_000233"
     CDS             complement(19747..20010)
                     /gene="ftsL"
                     /locus_tag="ACFOA3_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6897311.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(20007..20993)
                     /gene="rsmH"
                     /locus_tag="ACFOA3_000234"
     CDS             complement(20007..20993)
                     /gene="rsmH"
                     /locus_tag="ACFOA3_000234"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6897312.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(20990..21445)
                     /gene="mraZ"
                     /locus_tag="ACFOA3_000235"
     CDS             complement(20990..21445)
                     /gene="mraZ"
                     /locus_tag="ACFOA3_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6897313.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            21751..22941
                     /locus_tag="ACFOA3_000236"
     CDS             21751..22941
                     /locus_tag="ACFOA3_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6897314.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQSHFLLNL
                     GITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWAGFRFGDI
                     CHKL"
     gene            23073..23149
                     /locus_tag="ACFOA3_000237"
     tRNA            23073..23149
                     /locus_tag="ACFOA3_000237"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23107..23109,aa:Met,seq:cat)
     gene            23157..23232
                     /locus_tag="ACFOA3_000238"
     tRNA            23157..23232
                     /locus_tag="ACFOA3_000238"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23190..23192,aa:Ala,seq:ggc)
     gene            complement(23523..24344)
                     /locus_tag="ACFOA3_000239"
     CDS             complement(23523..24344)
                     /locus_tag="ACFOA3_000239"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6897315.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(24377..25021)
                     /locus_tag="ACFOA3_000240"
     CDS             complement(24377..25021)
                     /locus_tag="ACFOA3_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6897316.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(25024..25887)
                     /gene="ftsN"
                     /locus_tag="ACFOA3_000241"
     CDS             complement(25024..25887)
                     /gene="ftsN"
                     /locus_tag="ACFOA3_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6897317.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDATKEAEQSDAEKAADKQPIADKA
                     DEVEEKAGESEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(25993..27489)
                     /locus_tag="ACFOA3_000242"
     CDS             complement(25993..27489)
                     /locus_tag="ACFOA3_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6897318.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GNLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27503..27823)
                     /locus_tag="ACFOA3_000243"
     CDS             complement(27503..27823)
                     /locus_tag="ACFOA3_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6897319.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28306..29832
                     /gene="putP"
                     /locus_tag="ACFOA3_000244"
     CDS             28306..29832
                     /gene="putP"
                     /locus_tag="ACFOA3_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6897320.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            30083..33688
                     /gene="putA"
                     /locus_tag="ACFOA3_000245"
     CDS             30083..33688
                     /gene="putA"
                     /locus_tag="ACFOA3_000245"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6897321.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFLEWSATPTAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSTLAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGVQAVVEPDSPWASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         7985 a         9763 c         8391 g         7768 t
ORIGIN      
        1 tggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc gaatcaatca
       61 gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca taggcggaac
      121 gtctttggtg cggcggcata aaagtttaac cacggcataa ctgatgaagc cgaaagcgat
      181 gccgtctgca atcgaataag taaacggcat gaaaacaatg gtcaggaacg caggtgcggc
      241 ttccgtcata tcgtcccaat caatatcccg ggcactgcgg agcatttgtg tgccgacata
      301 aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg aaaacatcag
      361 gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc cgcctgccga
      421 tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag ccgcacctgc
      481 cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct taccgtccac
      541 cagcagcccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa atagatcgac
      601 caagaagaag acgaaaatca cactgaccat gctgacggta aacaggcctt caaaatccat
      661 ctgcataaaa gtcggcgcaa tgctcggtac ttcgccgatg atgccgtgaa attcattcaa
      721 acccatcagg ctggctatga cggtaatggt caagatggtg atgatgattg cgccttgaac
      781 gcggaaatgt cccaatacga ccaccatagc aaaaccgaac agtgccaaca acgcggacgg
      841 ctgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa tgcctgcgcc
      901 tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca ttttcaaacc
      961 cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca ggataaaaat
     1021 cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca tacccttaac
     1081 gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa tcggatagtt
     1141 gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag cgacgaatac
     1201 cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa cgatgtagca
     1261 catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg taccgtttgc
     1321 cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat ctgaaagtct
     1381 gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg caacatcttg
     1441 aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt acgggcaaac
     1501 agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt caaagtcgtc
     1561 aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga taccgcgatt
     1621 ggtgcggatc ataccttcga cattatggct ttgctcctgt ttggacgggg caaccgcttc
     1681 tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg tagcgataat
     1741 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gccgcaccga atttacattc
     1801 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact cggacatttt
     1861 caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt ccaaggttac
     1921 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     1981 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     2041 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     2101 cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta cgtcttcacc
     2161 caatgcagtc atcagtttgt cgttcgggat gataatcagc gaatcgacgt gttctttcaa
     2221 ctgttccaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga acggtcgggt
     2281 aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     2341 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg caccgcgaat
     2401 ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat cgggattcgc
     2461 gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     2521 ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc gcacattgtt
     2581 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaatcac
     2641 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgct
     2701 cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa gtatcttgct
     2761 gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag ctgttttcaa
     2821 tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc actgcaccgt
     2881 tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatagcgg
     2941 tagaaaaacg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg ggtgcaccgg
     3001 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     3061 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     3121 gctcgcccag cactacgcca aaaatctcct gaatccgtgc actgatgatt gctgccagaa
     3181 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcac
     3241 ccaagccttc cgtatcgcat gatgccacgc cataatggat tttaatgtac tcggcggcat
     3301 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
     3361 tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc gttccgccac
     3421 caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
     3481 ttgccaacgg ctgaagcatg atttgatcgc ttttcaagcc gcaccgctcg atacattttt
     3541 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccagacgca
     3601 caccgctcat accgatgggc tccctcacgc caagttgggt gtcgataatg tagtcttgaa
     3661 ccacggcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
     3721 cgcggtcgat gtctgcctgc gtgacttccc cgtctttaat tttaaccaca ccttgcgaat
     3781 tgagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
     3841 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
     3901 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg atgttgattt
     3961 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
     4021 cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg attgaaattt
     4081 gcgtcgcacc atttcagacg gcacggccgt aatctgtccg atacctgttc ccactattct
     4141 tcggattctt tttcgggtaa accgtcggaa gcatagcgga ctgaaaatcc gtccttatac
     4201 ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg ccacgcttcg
     4261 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
     4321 tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
     4381 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
     4441 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
     4501 tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc ggtcaggacg
     4561 acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
     4621 cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
     4681 ttcgccaaac tgcccaatgt cttcttatcg gaataaacca ggttgccctt cagcgacacc
     4741 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
     4801 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
     4861 atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgccca taacggcagc
     4921 ggatttcggt actaaactat ggccggtcat accgggcagg gtgttgattt ccaacagata
     4981 gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagccttccg caccgattgc
     5041 ctgcgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcaaatcttc
     5101 cgaaggacat tgataaatgg tgtcgtcgcg gttgtacttg gcttcgtagt cgtaaaactc
     5161 ggttgcggga atgatgtgta tgccgggcag ccctttgccg ttcaggacgg ggcaggaata
     5221 ttcgccgccg ccgataaaac gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
     5281 gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc tgctgccttc
     5341 ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcgacggcat cgaaatcagt
     5401 gtcgtcgtgc aggacggcga actcgggaac gggcaatccc aatgcctgcc aaatcagttt
     5461 gcagcggtat ttgtccatgc cgatggcgga tgcggcgaca ccgctgccgg tatagggaat
     5521 gcccaacagt tccaatgcac cctgaaccac cccgtcttcg ccgtaagtac cgtgaaggat
     5581 gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt ctttaggatc
     5641 gaaggcgtat gcgtctatgc ctttgctttt taaagcattc aaaatggcgg tgccgctgtc
     5701 cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt tgccaaaatt
     5761 ctgcattgtt ttttgttctt tcctgattgc tttatgcttg ttgccagagg tcgtctgaaa
     5821 cctgatttgc ggtttcagac gacctttata tgatgttccg tctgtcagga gggtgtgcct
     5881 caaatctgtt tcgacaattc cagtagcgcg gcggggacgc ggttgatgct tcccgcgccc
     5941 atattcaaca cgatgtcgcc gtcctgcaaa acgttcaaca gcatttcggg cagatcggca
     6001 acgttttcgc agtaaatcgg ctcgagtttg cccaacacgc ggatggcgcg ggcaagagcg
     6061 cgggaatcgg cggcggcaat cggctcttca ccggcggcat aaacttcggt cagcaccagc
     6121 gcgtcaacgg tattgaggac tttggtaaag tcttcaaaca aatcgcgcgt gcgggtatag
     6181 cggtgcggct ggaaggcgag taccaaacgt ttttccggat acgcgccgcg tgcggcggca
     6241 agggtcgccg ccatttcgac ggggtggtgt ccgtagtcgt ccaccaagag cgcggtcccg
     6301 ccgtttggca acttgatgtc gccgtatttt tggaagcggc ggccgacgcc ttcaaagccg
     6361 agcaagcctt tttggatcgc ttcaaccgat gcgccgactt ccagcgccac gccgatggct
     6421 gccaatgcgt tcagcacgtt gtgtctgccg ggcatattca gcacgacttc aaacgacccc
     6481 tgctcatgtc ctttcatttg aacatggacg gtgaatttca tttgcgcgcc gacgttttcg
     6541 atgtcggtgg cgtagatgtc ggcggtatcg tccaaaccgt aagtagcata aggtttgctc
     6601 actttgggca aaatcgcgcg gacgtgttcg ctgtcaatac acaaaaaggc tttgccgtag
     6661 aagggcatac ggtggatgaa atcgataaac gcctgatgca gtttttcgac gctgtgcccg
     6721 taggtatcca tatggtcttc gtcgatattg gtaacgacgg acataatcgg tgtcaggtgc
     6781 agaaaggatg catccgactc gtcggcttcg gcaacgatgt attcgccttt gcccaagcgg
     6841 gcgttggtgc ctgcggcgtt gagtttgccg ccgataacga aagtcgggtc aagtcctgcc
     6901 gcgccgagga tggaggcggt caggctggtg gtcgtggttt tgccgtgcgt gccggcaatg
     6961 gcgatgccgt cacggaagcg catcaactcc gccaacatca gggcgcgcgg aataacggga
     7021 atttgctgct ccaacgcagc gacaacttcg ggattttctt ttttgacggc ggtagaggta
     7081 acgacgacat ccgcaccgtt aacgtgttct gcggtatggc cgggataaac ttgaatgccc
     7141 aagctgccca aatgctcggt agcggcattt cgcgcctgat ccgaaccgga aactttaaag
     7201 cccaaattgt gcaagacttc ggcgatgccg ctcatgccga cgccgccgat accgacaaaa
     7261 tggatgttgg tcactcgatt tttcatcata atgttgcgtt ccggtggatt ttcgatgcat
     7321 aaaggcgtta ttttaaaggg ctgaccgttt gcgcgccata gttttctgac aaatatatag
     7381 cggattgaaa taaaaaacat ccatgctgtc tgaacggctt ttcagacggc atggttcggc
     7441 agtttacgcc gcacacgcaa tcgcggcttc ggcaacgtcg tccgcgctgt gcggcaatgc
     7501 caaagtacgg gcgttttctg cccatttgag acatttttcg cggttcaggc tgccgaggat
     7561 ttcggcgagt ttttccgccg tcaactgggt ttgcggcaac agcaatcccg cctccgcctg
     7621 caccataaaa cgcgcgttgg cggtttgatg gtcatcaacg gcgtgaggat acggcactaa
     7681 caacgcaccc aatcccgccg ccgtcaactc ggcaatcgtc agcgcgccgg cacggcaaat
     7741 caccaaatcg gcatcgcggt aggcggacac catgtcggta ataaattcca cacattcggc
     7801 tttcacgccc agcgcgtcgt aatccgcctg caagctgccc agcttgcccc gtcccgattg
     7861 gtggtacatc tgcggacgcg cattgtcggg cagcaaagcc aatgcctgcg gtacggtttt
     7921 gttcaaaacg tccgcgccca aactgccgcc gaccaccaaa attttcagac ggccttcacg
     7981 cccttggaag cgttcggcag gcacgggcag gttgctaata tcggcgcgga cgggattgcc
     8041 gaccaaaccg ccttcgtggc tgaacgcttt cggaaaagcg tacaacaccc gcttcgccca
     8101 gcgcgacagg tggcggttgg acaaacctgc cacggcgttt tgctcgtgaa tcacaatcgg
     8161 cacgcctaat agcttcgccg ccaaaccgcc ggggaaggta acgaagccgc cgaagccgat
     8221 gacgcactcg acacggtgtt tgcggataat ccgctgcgct tcgcggacgg tttgatacaa
     8281 agtaaccggc agcatcagtt tgcgtttgat gccgttgccg cgcacgcctt taatcgccag
     8341 cgtttccaag cgtatgccgt attgcggcac gatacgctct tccatcgaat ccttgctgcc
     8401 cagccaaatc acatgatggc cgcgcgcgcg caacgaatcc gccaccgcca gcgcggggaa
     8461 aatatgtccg cccgttccgc ccgccatcag cataaaggtt ttaccgccca tgatttactc
     8521 cacccgataa ccgcgcattt tccggcggtt ttcataatct atacgcaaca gcagcatcat
     8581 gctgatcagc atgaaaaaga ctgacgaacc gccataggac atcaacggca gcgtcagacc
     8641 tttggtcggc aaagcaccga tgttcacacc gatattgaag aaactttgga taccgatcca
     8701 aatgccgata cccgaagcga tataggcgtt gaaagtcaaa cccaaatcgc gcgactgctt
     8761 gccgatggaa aacgcccgca ccaccagcca gccgtaacag aatatcagca cgcacatacc
     8821 gaagaaaccg aattcttcgg cgatgatggc aaaaataaaa tcggtatgcg cttccggcag
     8881 aaagccgcgt ttgctcaaac tcgcacccaa acccataccg aaccactctc cgcgcccgat
     8941 tgccatcaga gagtgggtaa gctggtagcc ggcaccctgc gggtctttcc acgggtccaa
     9001 aaatgccact acccgctgca cacggtaggg agcggcggta atcatcagca ccatcccgcc
     9061 caagacgctg cctaccagga cgaaaaaata tttccacggc aatcctgcca aaaacagcat
     9121 tccaacggca atgacggtaa tgacgacaaa cgaaccgaaa tccggctgta ccattatcag
     9181 caccaaaccg aacgccacca gcataatcgg caggatgatc gcccggaaac ggccgtacat
     9241 ttctaatgtt tcacgacgtg cctgcggatt ggtggcggac atgatcagat tggccgtccc
     9301 ccgccaaatc gactgccaac ccaaactttc catgctgcgc aacacttctt cacggcgcgt
     9361 gaacaggctt gccaaataaa ggatgaccgc cagcttgaac agctcggtcg gctggaaatt
     9421 caacggaccc aaaggtatcc aacgggtcgc gccattgatt tcgcgcccgg caatcaatac
     9481 gactaccagc aacaggccgg ataaggcaaa aatccacggc acaagccgcc gccatgtcct
     9541 catcctgcaa agaaaccata aaaaaccgct cgctatcaag ccggcaacga cgaaccccgc
     9601 ctgtctggtc aaatagaaaa actgatcgcc gccttctttt gatgccaaat acacagaagc
     9661 cgaataaatc atcagcaggc tgaacgccgt catcagcacc accatccaca aaagcggcgc
     9721 gtcgaatttc ctgccgtcgc gcacaatctg cctgtcgagc agcagagtgt ggacaccgtc
     9781 gcccactttt accaatactt ccgaaatctt caaaaaacca cctgtcattc tgtttgcacc
     9841 tgccgcaaag ggcaaaaatt tcagacggca gacaatgccg tccgaacata cgatacatcc
     9901 caaatcggta ttctaaatct ttacttgccg cccaacaatg acggcgtttg catttcagac
     9961 ggcatcacaa agccttaaac gcttcgataa acacttccga acggtgcgcg tagcctttaa
    10021 acatatcaaa actcgcacaa gccgggctga gcagcacgat atcgcacgct tcggcttggg
    10081 catatgccgt ctgaacggct tcttccaaag tggcgcagtc ggtcatattc agaccgcagc
    10141 cgtccaaatc gcggcggatt tgcggcgcat caacgccgat caggaacacg ccttttgcct
    10201 tgccggcaag cgcgtcgcgc agaggcgtga agtcctgccc tttgcccatg ccgcctaaaa
    10261 tcacgaagag cggattttgc aaaccggcaa tcgcggcggc ggtcgcgccg acattcgtgc
    10321 ctttgctgtc gtcgatgaac accacgccgt ttttctcgcc gattttttcc acgcggtgcg
    10381 gcaggccttg aaaggttttg acgtgttcga gcaatgcttc gcgcggcaaa ccgacggctt
    10441 cgcacaaggc aacggcagcc atcacgttgg tagcgttgtg caagccttgc aacggaatat
    10501 cttgcgtggc aatcaaatct tcattgcctt gtttcaggcg gcctgtctcg cgttccaacc
    10561 aaaaatcggc ttcgtgttcc aacgaaaacc atttcacttc acgcccggcg cgcttcatgg
    10621 cgcggcagaa cacgtcgtcc gcattcaaaa cctgcacgcc atcgccacgg aaaatcttgt
    10681 ccttggtatg cgcgtagtcg agcaagtcgt cgtagcggtc gagatggtct tcggaaatat
    10741 tcagcaccgt cgccgccgtc gggcgcaggc tttcggtgtt ttccaattgg aagctggaaa
    10801 gctccaacac ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc tccaaaaccg
    10861 gcgtgccgat attgcccgcg ataacggtat ccagcccgca cttgatacag agatagccga
    10921 ccaggctcgt taccgtggtt ttgccgttgc tgccggtaat cgcaattacc ttgtcgcccc
    10981 ggcggttcac aatgtccgcc agcaattcga tgtcgcccaa cacgcgtccg ccgttttgct
    11041 tgaacgcctc aatatccggc tgccgctcgc tgatgccggg actgagagcc agaatatcga
    11101 aaccgttgtc cagcgcatct ttcagacggc ccgtgtaaaa caccaacccg tcaaacatct
    11161 taccgatttg cgacacgcgt tccggcttca gctccgcatc atacgcagca acctccgcgc
    11221 cgtttttgcg caggtaggca atcatggaaa tacccgtgcc gccaagtccg gcgacaagga
    11281 tttttttgtt ttggaaagtc attttggttt tgccctaaaa caaatcatat tgagcagggg
    11341 gggatgtccg ctcctgctca agccgctttc agacggcatc gcgagccgat taataacccg
    11401 ccttcaggcg ttggtcattg tcgcagccgt cttggtttcc gttttgacac gccttgccaa
    11461 gccattcttg agcgagcaca aggtcttggc gcacgccgcg tcctttgtaa tacattgcgc
    11521 ccaaactgta ttgggcttgg gctaacccct gtgctgccgc ctgcaagttt tcccgaaaat
    11581 ccgatacgtc atccgcccac accgcttggt tcaagcccaa ggcaatcagg gcggcggcaa
    11641 gccatttgac tgtctgtttc atggttttac ttctgtttta gtataagccg ccgttaacga
    11701 tagggctggg cggattgtcg ccgcaggttt attgcgcgtt caaatgccgt ctgaaagatg
    11761 ttcaaacggc ataggttcag cggattttga gggtactcaa gccgattaac accaagacga
    11821 tggtaataat ccaaaagcgg acgacgactt gggtttcttt ccagcctttt tgttcgtagt
    11881 ggtgatggat gggcgccatc aggaagatgc gttttttggt tttcttatac caaccgacct
    11941 gaagcataac ggatacggct tctacgacga acagaccgcc cataatcact aaaacaaact
    12001 cttggcggac gatgacggcg acggtaccga gcgcggcacc caatgccaat gcgccgacat
    12061 cgcccataaa gacttgcgcg ggataggcgt tgaaccacaa aaatccgagg cacgcgccgc
    12121 acatggcggt gcagaatata gcgacttcgt tcgcgccggc gacataggga agctggaggt
    12181 attgggaaaa ttggtagtgt ccgctgacgt aggcgaaaat ggcgaggccg gcggcaacga
    12241 ggacgacggg gaaggtcgca aggccgtcca agccgtcggt gaggttgacg gcattggatg
    12301 tgccgacgat ggtcaggtaa gacaacacca aaaagccgac cacgcccagc ggcagggcga
    12361 tttgtttgaa gaacgggaca atcaaaatat tgttggcgga attggcggca aggtaaaaca
    12421 atgccaaacc ggcgataatg gcaacgcttg actgccacac cattttgaat ttggcggaca
    12481 cgccgttggg gtctttatag acgactttgc gccagtcgtc gtaaaaaccg agcgcaccgg
    12541 tggcaagcag tacgcccaag agaatccaga tatacgggtt cgcccagttg ccccacaaca
    12601 gggtggacac ggtaatggcg gtcagaatca gcgaaccgcc catcgtcggc gtgccgtttt
    12661 tgacgaggtg ggtttgcgga ccgtcggtac gcactgcctg cccgcatttg agcgcggtca
    12721 gcctgcgtat cgtccacggg ccgaacatca gggaaaacgc caaggcggtc aacgccgcca
    12781 tgacggcgcg gaatgtggtg tattgaaaaa tattcaggcc ggttaaccag ttgctgaaat
    12841 gtgcgagcca taaaaacatg gggcttcctt tttttgtttt gtcgttatag tggattaaca
    12901 aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
    12961 gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    13021 atttttgtta atccactata catcgggcct gcgcccgttt gaggttggct tgccgcccgg
    13081 caaggtttcg gacggctttt gcagattaac gtgcagggac tttctcaatc acgcaaatcg
    13141 ggtaactccc cggatttttg ccgccaccca agtctaaaca taaatcttca taaagatacg
    13201 cctgcgtctg cattccagca taaaacgcgc cgaccagcgc ggcaatcaac aataaggctt
    13261 taaaaaaacg tcgacttttc attttcactt atcctccaat gcctcgacca cttcttccat
    13321 ctgcataaag cgcgaacctt tcaccaacac ggtggcgcgt tcgggcaaat cgtggcgcaa
    13381 cacttgaatc aacgggtctt tggcggcgaa ccacaaaccg tccgcgccaa attcttccgc
    13441 cgcttcgacg ctgttgtcgc cgacaaaata agccgcttcg atgccttggt cgcgggcgta
    13501 cgcgccgact tcggcgtgca tggcggcggc ttcgtcctcg cccagttcgc ccatatcgcc
    13561 catcacgaaa atacgcggcg caggcatacg caccaacacg tcaatcgcgg ctttcatact
    13621 gtcggggttc gcattataag tatcgtcaat cagggtcgcg cccttgatgc cggctttgac
    13681 gttcagacgg cctttgatat tgctgaagcc ttgcaaacct tccgccacat cgtccaaact
    13741 caaacctgcc gccaaagcca gcgcggcggc ggcggcggcg ttgtggacat tatggcggcc
    13801 gggaacaggc agcaccacgg cggcgcgttc gtttccgcac accaaatcaa attcgcacga
    13861 caacggtttc agcacgatat tttccgcgtg gacatcgccg ctatcgacgc cgaaagtgcg
    13921 cgtattcaaa ttaagcgttg ccgttttgaa gacagccata ttggcatctt cttgaggaat
    13981 cagcgccatg ccgtctgaac ataagcctgc ataaatctcg cttttcgctt tggcaatatc
    14041 gcccactccg tcgaaaccac agccgacatg ggcgcgcagg gcattgttgg ccaatgcggc
    14101 atcaggtttg gcaatttgcg tcaagaccgc cagttcgcca aaatggttca tgcccatttc
    14161 aatcacggca tagcggtgtt tttcgtttaa tttcaataaa gtcagcggca atccaatatg
    14221 gttgttgaag ttgcctgccg tcgccaaaac ggcattatcg ccgaaacggc ggcgcaatac
    14281 cgcagccaac atttccttca ccgtcgtctt gccgcccgag ccggtaatac cgaacacaaa
    14341 cgggttcaca ttctcgcgcc acgccttcgc caacatttgc aacgcggtaa gcgtgtcatc
    14401 gactttcaac gcgccatcca ttgcaacgca atcttcgcgc gaaaccacaa ccgccgccgc
    14461 accagcagcc aatacgtctt caacaaaatc atgcgcgtca aaccgcccgc ccgccaatgc
    14521 gaaaaacaca tcgcccgcgc ggatgtcgcg gctgtcggtt acgatgcgcg acacgggttt
    14581 gctttcagac ggcatcggaa gcttgagggc ttggcagatg aaatttagat ccagtggttt
    14641 catatttact ttcgttaata ttcgggcggc ggacacatcg gtagcggctg atttttttat
    14701 cgcctgtttt gctgtggtaa aacacagatt attttcccat tctcattcgg cattttttct
    14761 gtacgtatca ttttttagac gtatttttag tcgatttgcc ttttcccgca taccacggcg
    14821 cggggtcgtc gggcagtccg tcgataaagg caaggttatt gccttcgccc tgcacatcgg
    14881 gaacattccc ccaaaaatca tagccgtcat cgggcaactc gtcggtttcg atacccgtcc
    14941 aactgccgaa tccgcgtaaa aaattaaacg cgctgcgcct cactttaatc atttcgcgcc
    15001 gcgtatcatc ggacgaaatt ttaaccagcg gcacgccgta gctttgacgc ttccacgcac
    15061 catgtgtcaa cgttcgctcg ccgcctttac cgacatgcat caaaccgtgg tcggaaaagt
    15121 aaaccaaaga ccagctttct ttattttcat tcaatatctt aactgtatct tctaaaaatt
    15181 tatcggtttg cgcgatggtg gaaacatagc aggatatttt ttcagtttga tactgaaacc
    15241 gccgcgcatc cttatccaaa cgtgtgcaaa aatcactgtg cgaacccatc aggtgcatca
    15301 caatcagccg aggcttcgtg cctgcatttc ctatcaaaac ccgtttgaac gccggcaaca
    15361 aaaggcggtc gctcaacccc gcgcttttgc cataatcacc cctttgggta aaccacggat
    15421 aatcgctgcg tagggcatag gtggaaattt cgttggcaaa atgccccaac attccttgat
    15481 tagacagcca cgccgtccga aaacccgcct gcttcgccaa gctgacgatg ttattgttcg
    15541 gttctcccgg cagccccaaa gtctgcggca gcgaaagatt cgtcgcgtgg gcggtcgatt
    15601 ggtaaccgtt tatcaacagc cctttggtct gactcaaaaa aggcgtatcg ggcaatggga
    15661 aaccgtaaac attcatataa tccgaacgcg cgctctcacc gataaccaca acataatttt
    15721 tatacttggg cgcaacatga cggatatgcc atgttgacac tttttttgct gcttccaaaa
    15781 tgtgggcgcg cttggcggca tattgtgccg gcgcgaaagc caaatcgtaa tacaggtcga
    15841 aaatattcaa caacaggccg gcatcaggtt ctcgcaaatc tttatcgctg gcgattttat
    15901 ccatcaccgc gcaggacaaa atcaaagtca ataatatagt cagccatatt ttgctgcggc
    15961 gtttatagtt ttttacgtca gcaaataccc ccccccgata cacaatatct ccaaactgtc
    16021 aagccaaaaa tgaaaaataa tgcctgcaca aaataaagcg acccgggaag attgccgaca
    16081 aattcacgcg cctcggcagg attgctttcc aatatcgaac cgactatctg ataagacggc
    16141 gcaccataca accagccgac cggcaaatat agggcggttg tgccgacgta aatcagtaaa
    16201 acaactgaag aaacgcgcgg gaaaccgcgt gccaataata ataaaaatac aaccgaagcc
    16261 aaagcaccca cggcagaata acggtagccg tattcatatt ccaagtgata acctgatgcg
    16321 atggccgcgc caaaacaaaa ggctaaaagc gcgcaaatgc ccaatgtatt ttttaatgtc
    16381 tgatccatat tttttattcg ggcggattgt ttcatacgat gccgtccgaa tatcccatta
    16441 tttacgagtt aacaaagcct gtccgacgat ttcaagatcg gaaaaacggt gcttcacgcc
    16501 ttgtacatcc tgatagtttt catgcccttt gccggcaatc aggatgatgt cgtttgcggc
    16561 ggcttgttca accgcataac ggatggcggc ggcacggtcg gcttcgacgc attcgggcgc
    16621 gggaacggca ggcaggatgt cgttgatgat gtcgtgcgga ttttccaaac gcggattgtc
    16681 gctggtgacg acgactttat ccgcgccctg tacggctgcc gcgcccatca gcgggcgttt
    16741 gccgcgatcg cggttgccgc cgcaaccgaa tacgcaccac aaagccgcac cctgcggttt
    16801 gatttcctgc aaggtggcga gtgctttttc caatgcgtcg ggcgtatggg cataatcgac
    16861 aacgaccaaa ggtttgccgc tgttcatgat gcagtccatg cgccctgaag cgggacggat
    16921 ttttgccagc acatccaata cattatcaag cggatagccg ttggcgcaca gcaaggcgat
    16981 gcaggcggcg aggttttgcg cgttgaaccg tccgagcagg cgcgtgcggc atttcccttc
    17041 gccccacagg gtttggaata cggctgctat gccgtctgaa gaggcggtaa agtcggtaat
    17101 gcggatgtcg gcgtgttcgc tgaagccgta gctataaacg gccaaatcgg gacaatcttt
    17161 tttcagacga cctgcgagtt ccgcgccgta ttcgtcatct acattgataa cggcgtgttt
    17221 caagccgtgc cagtaaaaca ggcgcgactt gatggcaccg taggcttcca tcgtgccgtg
    17281 gtagtcgagg tggtcgcggg tgaggttggt aaagattgcg ctgcggaatg acacgccgtt
    17341 gacgcgcgac tggtcaagcc cgtggctgga gacttccatc gcggcgactg ttgcgccttg
    17401 ttgacggaaa cggtagagca gggtttggac atcgacgggg gcgggtgtgg tatgcgtggt
    17461 ttcttccaat gcaccccaaa agccgttgcc gaccgtgccg acaatggcgg ttttttcgcc
    17521 caacaaatcg gcagcttgcg ccagccattg cgtgatggag gttttgccgt tggttccggt
    17581 tacgccccag actttgagac cgtctgaaac gttgccgtaa acttgcgccg ccaatatgcc
    17641 ggcacggtgt ttcaaatctt tgatgccttg attggggact ttccattcgg gattccacgc
    17701 gaatttgcca tcgtcgtccc aaaaaacaaa agccgcgccg ttggcaacgg cggcggggat
    17761 ataactgcgt ccgtcggcat attcgcccgg acaggcaacg aaaatatcgc cttgtttgat
    17821 ttggcggctg tctgaatgca acaaacgccc tgccgcgttt gcacacgaca gggtcgggat
    17881 gccggtttca gccaaagggg ttaacttgct gaacataaaa caatctcgtt gatactcgga
    17941 ttaagacggt gttttgacgg ctgcggcggt cagtggcttg gtcggggaaa tgcccaagat
    18001 gttcaggctg ccgcccataa tttttttgaa gggcggccct gccactacgc cgccgtaata
    18061 accgtgggca gtcggttcgt caatggttac cgccacaatc acacgggggt ttttggcggg
    18121 ggcaaaaccg ataaaggtag cgatgtgttt gttgtcggca taacgcccgt tgacgaactt
    18181 gcgcgccgtg ccggttttcg cgccgacatc gaaaccgtcc accgcacccg ccgtaccggt
    18241 gccgcccggc tcggtcacgg aaaccatcag attgcgtacc tcgcgcgcgg tcgattcttt
    18301 gaatatgcgt ttgccttgcg gcgcaaccgc ctgtttttca aagctgaccg gcagcaaaac
    18361 gccgtcgtgc gtcagtgcgg tataggcgcg cgccaattgc agcaggctca attgcaggcc
    18421 gtaaccgaaa gacatcgtcg cctgttcgat gggccgccac ctgcgccaat ttctcaacaa
    18481 acctgcagtt tcgcccggaa agcccgagtg catacgcaca ccgatgccca actcatgata
    18541 gaagtcatac atttcttcgg caccgaaacg cgcagacagt ttgcttgtgc cgacgttgga
    18601 cgatttctgc atgatgccgc gcacatccaa agaggggtaa acatgggtat cgcgcacggg
    18661 agacggtccg attttataag gctgcgtatt cagccgttcg ttcaaatcgg ttttgcccgc
    18721 atccaatgct ttcgcaatca caaacggttt gattgccgaa ccgggttcga tcatatcggt
    18781 tacggcacgg ttgcgccgct gttcgctgtc tgcccggccg ggcctgttgg gatcgtaggc
    18841 gggcgtattg gccaaggcga ggatttcccc cgtgcgggca tccaaaacca ccaccgttcc
    18901 ggcttttgcc tgatggtatt cgaccgcctt gttcaactct tcataggcca aggtctgaat
    18961 cctctgatcg agggaaagga tgatgtcttt gccgtttttc ggggctttat tgcgcgggga
    19021 gtccaagctg tccacaatat tgccctgccg gtcccgcaaa acgacttccg cgccgtcttc
    19081 gccatgcagg ctgtcttcaa gcgaaagttc caaaccttcc tgacctttgc cgtcaatatc
    19141 ggtaaatccg atgacgtgtg caaacaggtt gcccatcggg taatggcgtt ttaattcttt
    19201 ttcaaataca aagttttcca aacccaaggc tttgacctct tcggcaacct tgggatcgag
    19261 ctgccgctta atccagataa acgacttgcc tttctgttcg agcttgttcc tcaaaacatc
    19321 aatcggaaca tcgacaagct cggacaggcg ttccaattgt gcggcagacg gcatttcctt
    19381 catctcttta ggcacggcaa acagggactc cgtcggcgca ctcaacgcca aaaccgcacc
    19441 gttccggtcc gaaaccgtac cgcgtgtagc cggcaatgtt tgagtccgca caatccggtt
    19501 gtcgccctgt tctttcaaaa agttatacgt taccgtctgc agatacagtc cgcgggcaat
    19561 cagaccggca aacaagaccg ctattgccat caggacgaag ctgatccgtc cgttactggt
    19621 catcggcttt ttgacctgct cttctttggg cagcatccgg ggcttatatt cactcttaat
    19681 caacattttt acttctcgtt attattatcc tgacgcagga atccgattcc ggcacacagg
    19741 ctgcttctat ctttgatgcg ccaccataaa ggtattgccc gaaaccggcg gatggaggtt
    19801 ttgtttttct gccgccgccc tgatcgcttc gtggttcgcc aaacgcgcct gttgcagcct
    19861 catttgcgca taatcctgct ccaaggcgat ttcctgtttt ttcgccttat ccaaagctgt
    19921 gaagttgagc ctgtactggt tttgctgcat cacaacggaa aaagcggaaa cgcacaccgc
    19981 aagcagcaga aggaaattca atttgttcat tgccattcag acgtttttct ctgtgattgt
    20041 tccggtatcg gaccggcagt ccgctccgcc acacgcaaaa ccgcacttct cgccctcgga
    20101 ttggcggcaa tttccgcctc acccggcttt aatgccctgc ccgcgatttt caggggcggc
    20161 tcgggcaaat ccgcttccct gaccgccgcc cagcgcggca ggggcgcgtg ttgcgaatat
    20221 tttttgacaa actgcttcac aatgcggtct tccaacgaat ggaaagcaat gaccgccaaa
    20281 cgtccgccct ctttcagacg acacatgacc tgcggcaata ctgcccctac ttcttcaagc
    20341 tcgcggttaa taaagatgcg gattgcctgg aaggtgcgcg tcgcaggatc ctgcccccgc
    20401 tcgcgagtac ggacgttttg tgccacgatc tgcgccagct tgcgggttgt atcgattgga
    20461 ctttccgccc gttgcgcaac aatggcgcgc gcaatctggc ggctaaaccg ctcttcacca
    20521 taattcttga ttacctcgtg caaatcctgt tccgacgcaa ccgctatcca ctctgcggca
    20581 gacataccgc gcgtcgtatc catacgcata tccaaagggg catcgaaacg gaagctgaaa
    20641 ccgcggctgc cgtcatcgat ttgcggggac gaaatcccca aatcaaacag cgcaccgtcc
    20701 accttgccga tacccaaacc gtccaatgcc gtctgaaacg aagcaaaacc gccatgcacg
    20761 acaccgaccc gtttgtccga acgcgccagc tcttctgcca cagcaatcgc ctgcggatct
    20821 ttgtcgaaaa caatcaaccg ccccgcatcg cccaaacgcg acaaaatcag ccgggaatgc
    20881 cctcccctgc cgaacgtacc gtccacatag acaccgtctt cgcgcacggc aagcgcatcc
    20941 accgcctcat tcagcaagac cgtgatatgc cggtaacttt ctgctccact cacaattgca
    21001 aatccgtctg acccaactgg aaggcaagtt cgtcaggatc gtcatccaaa gcctgaacca
    21061 tctcagcctc ccactgctcg cgaccccaaa gctccaaacg gttggcacgg ccgaccaaaa
    21121 cgacttcacg gtcgaaatcc acccttttcc tcagtccggc aggaagcagc acccggccgg
    21181 cgctgtccca ttccaaaatt tccgcgttat gcagcaaaag attttgaaac cgccgcaaaa
    21241 cagggttatc cgccactttt aagtttaaaa gttgcgccgc aaccttttcc cactccgcaa
    21301 cagggtacat caacagcttg tgtttcgact cgagcgttac cactacggca ggcgtataga
    21361 ggcgcgacag aatgtcacgg aatttggcag gaacagccaa ccgcccctta ctgtcgatgc
    21421 ttaattcgtg tgcgccgccg aacatgacat gtcccaagcc gaaatcaaaa tcacaagggt
    21481 aaaagagaca ctttgcccca caattcccca ccaatcgaca ctataagaaa ttttaaacac
    21541 tcggtcaaat cagggcatga aaactcatta acatatctga aatttttatt ccttttaaaa
    21601 acaataagat aaaaaatgac gacaacggca cggcgggtgc ggtacagaat aatcgaacca
    21661 ataaacaact atatatgatt aatttaataa tataaacaca atatatagta ttaagataaa
    21721 gccatgacag cacccgtacc aacgtgtaat atgtcgggaa atccaataaa tttacacaag
    21781 ctaacactta tcatgcccct ccccctcccc tctcccgaag cacggcaatt ctcgctcaaa
    21841 ctgcaaaccc tcattgccga aaaaatcggc aaacacggca actggattcc attttcacgt
    21901 tttatggaat tggttttata cgctccgcaa tacggctact acaccggcgg cagccataaa
    21961 atcggcaata ccggggattt tattaccgca ccgaccctca cctctctgtt tgcacagaca
    22021 ctggcacgcc aacttcaaga acttctatcc caaacggcgg gcaatatcta tgaattcggc
    22081 gcgggaaccg gacagcttgc cgccgatttg ttgggcagca tttcggacgg catcagccgt
    22141 tactatatta ttgaaatatc gccggagctg gcagcacgtc agaaaaacct gattcaagca
    22201 cgcgcaccgg aagcatctca aaaagttgtc cacttgaccg cacttcccga agcgtttgac
    22261 ggcatcatca tcggcaacga agtactcgat gccatgcctg tcgaaatcgt ccgtaaaaat
    22321 gaaggcggct cattcgagca tgtcggcgtt tgcctagata atgaccgttt tacctattcg
    22381 gcacgaccgc tgcacgactt acagctatct gccttggctt ccctctattt tcctcaaaca
    22441 gattatccct acaccagcga actacatccg caacaatatg cctttatccg cacccttgcc
    22501 tccaggcttg aacgcggggg tatgatattc atcgactacg gttttgatgc agcgcagtat
    22561 taccaccctc aacgcaatca aggtactctg atcggacact accgacatca cgttatccac
    22621 aatccttttg acttcatcgg gttggccgac ctgaccgcgc atgtcaactt taccgacatt
    22681 gcccaagccg gtacggatgc aggactggat ttgacaggct atctgcccca gtcccatttt
    22741 ctgctgaatc tgggcataac cgaactgttg gcacaagtgg gaaaaacaga ctcgcccgcc
    22801 tacatccgtg aagctgctgc cgttcagaaa ctgattgacc agcatgaaat gggcgaactg
    22861 tttaaagtca tcgcattcgg aaaaaatatc ggcatcgact gggcaggatt ccgcttcggc
    22921 gacatctgcc acaaactcta accctcatgc cgcctgaatc cgcttcagac gacataaact
    22981 ttttaacatt taaaaacagt caactaattc aaaattaaaa aatacggctt gtcaaaaaaa
    23041 cagaaaaaca tataatagcg tcttcacgaa acggcgaatt agctcagtcg gttagagcag
    23101 aggaatcata atccttgtgt ccggggttcg agtccctgat tcgccaccaa attttcgggg
    23161 gtatagctca gttggtagag cgcttgcatg gcatgcaaga ggtcagcggt tcgatcccgc
    23221 ttacctccac cagataaaaa agcacagacc gtaaaaaggt atgtgctttt ttattgcctg
    23281 attgccggca aataaagaat aaaccactgc cttcaaaaca ggcaatcgac tttaaaccta
    23341 tcgccccgcc tgtcctgatt ttatagtgaa ttaaatttaa accggtacag cgttggctca
    23401 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatt
    23461 cactatatca gcccgccaga caaacccgac ccgaataatg tcttcaggtc gggtttatgg
    23521 tttcattccc aacttatcca gcctgacagc cacaatataa tgatggcaat accaaaaaca
    23581 atgcggtaat aggcaaaagg aatataattt ttcttggaaa caaacctcag caacgctttt
    23641 accgctacca agcctgaaac aaaggcagca acaaagccaa tcaaaatcaa accgacatca
    23701 tgcagggtga aaaaccggta atgcttcagg acatcataag ccgttgcggc aaccatcatc
    23761 ggcacagcca agaaaaacga gaattctgtc gcagttttcc gttcgattcc ccaaagcatc
    23821 ccgcccataa tcgtactgcc cgaacgggac gtacccggaa tcagtgcaaa cacttgggca
    23881 acgccgatca tcaaggcatc aatcggacgc aatgcatcaa catcggcaat tttaggctct
    23941 gctcggcttt ggcgtttctc cacccacaaa ataaaaaaac cgcccaaaac cagcatgact
    24001 gcaacactca aggggttaaa cagatactct ttgatttgtt tgccgaacaa cagccccatc
    24061 acggcggcag gtataaaagc aatggcaaga ttgaggacga agcggttggc tttccggtct
    24121 tttcccacgc catgcaacac attgctgaaa cgttgccggt attcaaacac taccgccaaa
    24181 accgcaccga gctggatggc aatttcaaaa accttgtgat tgctgtgaaa accaatcaga
    24241 ttgccgaaca caatcaaatg cccggtgctg gaaatcggta aaaattcggt aaaaccttct
    24301 accaagccca tcatcagggc tttcaggaca atcagaaaat ccattgcttg cgcttctttc
    24361 ggatacggga gttcggctat ttctgtacag caggggtctg acgcttgcgt tcttccctga
    24421 ctttggcaac caattcttta atcgtatgaa cgccgccgtc aaagccgtta ttgaagataa
    24481 cgcggtattt tccgccgaca ataacggtcg gcgtgctgtc aataccgtat tgttccgtca
    24541 gtttctgcat ttttaatgcg gcggcggcag cttcgggaga atcataggcg cgcatcagtt
    24601 ttttgccgtc aaagcctttt tgagacaaag cccactttcc ggcaaccgac ctgttttcca
    24661 agcggatttt ttgttcgtaa actgctttaa acacagcagg gtttgcctga tatttcaaac
    24721 ccgacaaatt gacggcagcc gccatcctgg ccagaccgag catttcaggc tgccagacca
    24781 cgtgctccgt cctcaaatag gcatcagacg gcaatgcctt gcccaatttc aataacaaag
    24841 gatcgaaatg atggcaatgt acgcagaaat agccgaaaaa ttccaaaacc tcaattttgc
    24901 ccgactgttc ttgaggaatg ggtttatcca acacaagata gtcttccctt tccgtcaggg
    24961 catatgcctg cgcggacaac actgccgaca gcagcagcgg caacagatgt ttgagcttca
    25021 taattatttg ctttcgatag aacggatcag gctggcgact tcatgttttt tcaactcgtc
    25081 ctgcattttt ttcaccgcat cggcagacat attgccgctt tgcacccggt aaagcgtttt
    25141 atgtcccgcc tgataaccga ccaccttgga agatatgccc aagattgcca gtttggcacg
    25201 ctgtccttcc gcgctccggc ggtcggcata cgcgcccatt tgcagataat gcgttgcttc
    25261 cgccttgtcg ggcgttttca ttttctgcac ttctttggca gcggcactgc gcgctttttc
    25321 gatgctgccg ctgttgagga tttgttctgg ggtcggtttg ggtgcaactt tttccttttc
    25381 cgcctttttc tcttcttttg aagctttttt ctctgtttct ttagacggtt ttaccgcttg
    25441 ttttttaacc gtttcggcat ctttcttctg cgctttttcc ccgacggttt gttcacgctc
    25501 ttccgtcagt gctttcttac gcactgcctg cccgtccgac ttttcccgct ccgactcgcc
    25561 cgccttttcc tcaacctcgt cggctttgtc ggcaatgggc tgcttgtcgg cagctttttc
    25621 cgcatccgac tgctctgcct ctttcgtagc atccggttcg gacaaggcgt tttgatcggc
    25681 cggttcaggt tggatgtctt ccttaggctg gtttttcggt ttcaggattt ccgtttctgc
    25741 aggctgcttc gacgaagccg ggattttgaa cgcattttga ccgctctggt tcagataaaa
    25801 caaaataccg gcaataatga ccgtcgccag tatcaaaccg aagaaaaaac cggacaaacc
    25861 ttttccggat tgggaaaatt tgttcataaa cataccttaa tgtgtttcag acggcattaa
    25921 cgccgtttgc ttacgggcgg atattctaac aatatcgcca tatttgggca aaacctgctt
    25981 ccattcccgt tcctataaag cgcggcggaa acctatggct ttcatgacgt ggcttctgcc
    26041 gacttcttcg tcgcctgcca aatccgccaa tgtacgcgcc acgcgcataa tgcggtggaa
    26101 gctgcgggcg gagagggaga gtttttccaa caggttgccc aaggcttcct gcgcttcttt
    26161 ttggatgcgg gcttgggaat cgagttcgct gacgctcaaa gcggcgttga ctttgccctg
    26221 ccgtgcgtat tgtttgtcgc gggcggcgat aacgcgttcc aaaacggacg cgctgctttc
    26281 ccctgcttcc tgctgcatca gttcggcggc ggacaggctg ggaacttcga tggtcaaatc
    26341 gatgcggtca agcagcgggc cggaaatctt gctgcggtaa cgcgcgacgc tttcgggcgt
    26401 gcaacggcag ggtttgacgg gatgcccgag ataaccgcac gggcaggggt tcatggcggc
    26461 gacaagctgg aatttggcgg gatagacggc ttggcgcgcc gcgcgggaaa tgtggatttc
    26521 gccgttttcc aaaggttcgc gcaacacttc caaaactttg cggtcgaact cgggcaattc
    26581 gtccaaaaac aaaacgccgt ggtgcgccaa tgaaatctcg cccggacgcg gatccgagcc
    26641 gccgccgacc atagccgccg cgctggcgct gtgatgcgga ctgcggaaag gacggttgct
    26701 gtcgagttgt tgctggtggt tgggcaggag cgaacgcaat gcccaaactt caaccaattc
    26761 gtcttcggtc agcggcggca ggatgcctgg cagccgttgg gagagcatag acttgcccgt
    26821 tcctggcgga cccatcatca agaggctgtg tcctcctgcg gcagcgattt ccaaagcaag
    26881 gcgcgcggtg tgctgacctt tcacatcgca caaatcaggt tgtccgccat gttcaaacgg
    26941 catctgagga acttggcatt cggtttgcgc caaaggttcg atgccgttca aatgggcggc
    27001 gacttcgccc aaagagcgcg cgccgtaaac ggtaatgccg cgcatcacgg cggcttgtcc
    27061 tgcattttct tcaggcaaaa caaatgcacg ttttgcctgc ataccctgcc acgccatcgc
    27121 caacgcgccg cgcacggggc gcaacagccc cgacagtgcc aattcccccg caaactcgta
    27181 ttccgccagt ttttcgggcg caacctgccc cgatgcggca aggataccga ttgcaatcgg
    27241 caaatcgaaa cgccccgact ctttgggcag gtcggcgggg gcgaggttga cggtgatttt
    27301 tttggcgggg aattcaaaac cgctttgaat aatggcggca cggacgcggt cgcgactttc
    27361 tttgacttcc gtatcgggca gcccgacgat gttgaaatgc ggcagaccgt tggcaaggtg
    27421 ggcttccact tcgaccaacg gcgcattcat accgctcaag gcgcggctgt aaaccaaggc
    27481 aagcgacata tttcagacgg ccttattcgc cggcttcggt ttgctgcctg atttcggcga
    27541 cggcttcttc ggcagcggct tcagccgctt ccaatgctgc ccgttcggga ttttgcgcgg
    27601 cttcgagttt tgccaaacgc gcttccaaag ccgccagttt ggtacgggtt ttgattaaaa
    27661 cctgctgctg gatgtcgaat tcttcgcgcg taaccagatc catacggttg aacgcgccgc
    27721 ccagcatcgc cttaatattt ttttccacat ctttggcagg gctgttggcg atggtttcgc
    27781 tgattttcga gccgacttcc tcaaaaagct gcttgccgaa cataatctgt atccttcctg
    27841 aacatatcaa attcaatcgg ctattgtata aggaaaaatg ccgtctgaaa acgggcggcg
    27901 gataatcggc aaaacatacc gcgcctcctt tgcggttgct aaattacacc tcaaaacacc
    27961 tgccgcctga aaacggattt catattccgc gcaacgcctg attacaagac actacaaaac
    28021 aatatgctgt tttaaatgat ttttccgacg cgcatcgttt caaactcggc tttttaagcc
    28081 attaagtgct ttgcaaacaa cagaaattgg gttatgctga aacggattat ttacaatttc
    28141 atatagtttt attacatatc ttattgtgat tgaagataat ttatccgaat ccccctttcg
    28201 ggtatccgga ttttccgttg tacttttatt agaaaaacat ttcaggcgca agttgcttgc
    28261 aatttcaaag cctgtaattc tgaagtttta gacgaggaat agcacatgaa tcccatgtac
    28321 attacttttg caatctatct ggttgcagtc cttctcatcg ggcttgccgc ctatttttct
    28381 acgcgcaatt tcgacgatta tattttgggc gggcgcagcc taggcccgtt tgttaccgcg
    28441 atgtcggcag gcgcgtccga tatgtccggc tggctgctga tgggtctgcc gggcgcgatt
    28501 tacctgagcg gtttgaatga ggcttggatt gccatcggcc tcttggtcgg cgcgtatttc
    28561 aactggctct tggtggcggg ccgcctgcgc gtgcataccg aatacgccaa caatgcgctg
    28621 acgctgccgg attatttctt ccaccgcttc ggcgcgggcg gacacttgat gaaagtggtt
    28681 tccgcactga ttatcctgtt cttcttcacg atttattgcg cctcgggcat tgtggcgggc
    28741 gcaaccctgt tccaaagcct gtttgaaggt atgacttaca atcaggcaat gtggctgggc
    28801 gcgggcgcga ccatcgccta taccttcttg ggcggtttct tggcggtcag ctggacggat
    28861 acgctgcagg cttctttgat gattttcgcc ctgattttaa cgccggtgat ggtctatctg
    28921 ggcttgggcg gcgcggaaca gatgtctgcc gcgattcaaa gcgtcgccgc aggcacgggc
    28981 aaagaatacg gcagcctgtt tgccggtacg accgtcatcg gcatcatttc caccgccgca
    29041 tggggcttgg gctatttcgg acagccgcac attttggcgc gctttatggc ggccgaaagc
    29101 gcgaaatcgc tggtatccgc acgccgcatc ggcatgactt ggatggcgtt gtgccttgcg
    29161 ggcgcggtag cggtcggtta tttcggcatt gcgtatttcg gtgcaaatcc cgacaaagtt
    29221 tcttctatga gcggcaacca cgaacgcatc ttcatcgcgc tttccaccct gctgttcaac
    29281 ccttggatcg ccggcattat tttgagcgcg attttggcgg cggtgatgtc caccctgtcc
    29341 tgccagcttt tggtttgctc cagcgcgatt accgaagact tctacaaagg ctttttgcgt
    29401 aaaaacgcgc aacagtcgga actggtatgg gtcggccgcc tgatggtctt ggcgattgcc
    29461 gtgatttcca tcctgattgc ttccgatccg aacagcaaag tattgggact ggtgtcttac
    29521 gcgtgggcag gtttcggtgc ggcattcggc ccgattgtga ttctgtccgt attgtggaaa
    29581 cgcatcactg cctacggcgc gttgtcgggt atggttgcag gtgccttaac cgtggtcgta
    29641 tgggcggaat gggtgaaaaa accggctcaa gcggcaggcg aaagcggctt gttgacgatg
    29701 tacgaaatcg tcccgggctt tattgtttgc ttgatcgttg ccgtgttggt ttccctgttc
    29761 aacaaagaac cttcgcgcga aacccaagaa cgctttgaaa aagccgacgc agattaccgc
    29821 gccgcccgat aaaccccttt cagacggcac ggcatcccgg ttcgtgccgt ctgaacccgt
    29881 ttccgggtca aacacaaata aaccttgcct gcatacaggc cttatttgtg ttttacccgt
    29941 ttcattttca aagaaacgga agcacctacc ctgcccgcat ttgaagcctt gtatttcatc
    30001 tgcggattta ttacgatatt accgtattca gaccgcaccg atgccgtctg aaccccccga
    30061 aaaactttgg agaatccaaa aaatgtttca ttttgcattt ccggcacaaa ctgccctgcg
    30121 ccaagcgata accgatgcct accgccgtaa tgaaatcgaa gccgtacagg atatgttgca
    30181 acgtgcacag atgagcgacg aagagcgcaa cgccgcctcc gagcttgccc gccgtttggt
    30241 tacccaagtc cgcgccggcc gcaccaaagc cggcggcgtg gatgcgctga tgcacgagtt
    30301 ttcactctcc agcgaagaag gcatcgcgct gatgtgtctg gcagaagccc tgctgcgtat
    30361 ccccgacaac gccacgcgcg accgcctgat tgccgacaag atttcagacg gcaactggaa
    30421 aagccatttg aacaacagcc cttccctctt cgtcaacgcc gccgcctggg gcctgctgat
    30481 taccggcaaa ctgaccgcca caaacgacag acaaatgagt tccgcactca gccgcctgat
    30541 cagcaaaggc ggcgcaccgc tcatccgcca aggcgtaaat tacgccatgc gcctgttggg
    30601 caaacagttc gtaaccggac agaccattga agaagccctg caaaacggca aagaacgcga
    30661 aaaaatgggc taccgcttct ccttcgatat gttgggcgaa gccgcctaca cccaagccga
    30721 tgccgaccgc tactaccgcg actatgtcga agccatccac gccatcggca aagatgcggc
    30781 aggacaaggc gtttacgaag gcaacggcat ttccgtcaaa ctttccgcca tccatccgcg
    30841 ctactcgcgc gcccaacacg gccgtgtgat gggcgaactg ttgccgcgcc tgaaagagct
    30901 gttccttttg ggtaaaaaat acgatatcgg tatcaacatc gatgccgaag aagccaaccg
    30961 tctggagctg tctttggatt tgatggaggc tttggtttca gaccctgact tggctggcta
    31021 caaaggtatc ggtttcgttg tccaagccta ccaaaaacgt tgtccgttcg ttatcgacta
    31081 cctgattgac ctagcccgcc gcaacaacca aaaactgatg atccgcctcg tcaaaggcgc
    31141 gtattgggac agcgaaatca aatgggcgca agtggacggc ttgaacggct atccgaccta
    31201 cacccgcaaa gtccacaccg acatctccta cctcgcctgc gcgcgcaaac tgctttccgc
    31261 gcaagacgcg gtattcccgc aatttgccac gcacaacgcc tacaccttag gcgcaatcta
    31321 ccaaatgggc aaaggcaaag actttgaaca ccaatgcctg cacggcatgg gcgaaaccct
    31381 gtacgaccaa gtcgtcggcc cgcaaaactt aggccgccgc gtgcgcgtgt acgccccggt
    31441 cggcacacac gaaaccctgc tcgcctactt ggtgcgccgc ctgttggaaa acggcgcgaa
    31501 ctcgtctttc gtcaaccaaa tcgtcgatga aaacatcagc atcgacacgc tcatccgcag
    31561 cccgttcgac accatcgccg aacaaggcat ccacctgcac aacgccctgc cgctgccgcg
    31621 cgatttgtac ggcaaatgcc gtctgaactc gcaaggcgtg gacttgagca acgaaaacgt
    31681 attgcagcag cttcaagaac agatgaacaa agccgccgcg caagacttcc acgccgcatc
    31741 catcgtcaac ggcaaagccc gcgatgtcgg cgaagcgcaa ccgattaaaa atcctgccga
    31801 ccacgacgac atcgtcggca cagtcagctt tgccgatgcc gcgcttgccc aagaagcggt
    31861 tggcgcagcc gttgccgcgt tcctcgaatg gagtgcgaca cctaccgccg aacgcgccgc
    31921 ctgcctgcgc cgtttcgccg acctgctcga acagcacacc cccgcgctga tgatgcttgc
    31981 cgtgcgcgaa gcgggcaaaa cgctgaacaa cgccattgcc gaagtgcgcg aagccgtcga
    32041 tttctgccgc tactacgcaa acgaagccga acacaccctg cctcaagacg caaaagccgt
    32101 cggcgcgatt gtcgccatca gcccgtggaa cttcccgctc gccatcttta ccggtgaagt
    32161 cgtttccaca ttggcggcag gcaacaccgt tatcgccaaa cctgccgaac aaaccagcct
    32221 gattgccggt tatgccgttt ccctcatgca cgaagccggc atcccgactt ccgccctgca
    32281 actcgtcctc ggcgcaggcg acgtgggtgc ggcattgacc aacgatgccc gcatcggcgg
    32341 cgtgattttc accggctcga ccgaagtggc gcacctgatc aacaaagccc ttgccaaacg
    32401 caacgacaat cccgtcctga ttgccgaaac cggcggacaa aacgccatga ttgtcgattc
    32461 caccgcactt gccgagcaag tctgcgccga cgtattgaac tccgccttcg acagcgcggg
    32521 acaacgctgc tccgccctgc gcattttgtg cgtccaagaa gacgttgccg accgtatgct
    32581 cgacatgatc aaaggcgcga tggacgaact cgtcgtcggc aaaccgattc agctcactac
    32641 cgatgtcggc cccgtcatcg atgccgaagc acagcaaaac ctgttgaacc acatcaacaa
    32701 aatgaaaggc gttgccaagt cctaccacga agtcaaaacc gccgccgatg tcgattccaa
    32761 aaaatccacg ttcgttcgcc ccatcctgtt tgaattgaac aacctcaacg aactgcaacg
    32821 cgaagtcttc ggccccgtcc tgcacgtcgt ccgctaccgc gccgacgaac tcgacaacgt
    32881 catcgaccaa atcaacagca aaggctacgc cctgacccac ggcgtacaca gccgcatcga
    32941 aggcacggta cgccacatcc gcagccgcat cgaagccggc aacgtttacg tcaaccgcaa
    33001 catcgtcggc gcagtcgtcg gcgtacagcc cttcggcgga cacggtctgt ccggcacagg
    33061 ccccaaagca ggcggttcgt tctacctgca aaaactgacc cgcatccccg aatgggttgc
    33121 cccgaccctg agccaaatcg gacaggcgga cgaagccgca ctcaaacgcc tcgaagcact
    33181 ggttcacaaa ctaccgttca acgccgaaga gaaaaaagcc gcagcggccg ctttgggaca
    33241 cgcccgcatc cgcaccctgc gccgtgccga aaccgtcctt accggcccga ccggcgagcg
    33301 caacagcatc tcatggcacg cgccaaaacg cgtttggata cacggcggca gcacggttca
    33361 agcctttgcc gcactgaccg aacttgccgc ctccggcgta caggcagtgg tcgaacctga
    33421 cagcccctgg gcttcctaca ccgccgactt ggaaggtctg ctgctggtca acggcaaacc
    33481 cgaaaccgcc gacatcagcc acgttgccgc cctgtcgcct ttggacagcg cgcgcaaaca
    33541 ggaacttgcc gcccacgacg gcgcactcat ccgcatcctc ccttcggaaa acggactcga
    33601 catcctgcaa gtgtttgaag aaatctcttg cagcgtcaac accacagccg ccggcggcaa
    33661 cgccagcctg atggcggttg ccgactgatt ttaccgaaat ccccgagcgc ggcccgtgaa
    33721 ccaatgccgt ctgaaaacct ttcagacggc atttttatag tggattaaca aaaatcagga
    33781 caaggcgacg aagccgcaga cagtacagat agtacggaac cgattcactt ggtgcttcag
    33841 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
    33901 atccgct
//
LOCUS       DAXIEB010000006        33787 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000006 DAXIEB010000000
VERSION     DAXIEB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33787
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.006"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(127..1083)
                     /gene="gshB"
                     /locus_tag="ACFOA3_000246"
     CDS             complement(127..1083)
                     /gene="gshB"
                     /locus_tag="ACFOA3_000246"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6897322.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1176..2864)
                     /locus_tag="ACFOA3_000247"
     CDS             complement(1176..2864)
                     /locus_tag="ACFOA3_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6897323.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVLELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPTESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(2976..3749)
                     /locus_tag="ACFOA3_000248"
     CDS             complement(2976..3749)
                     /locus_tag="ACFOA3_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6897324.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3792..4694)
                     /locus_tag="ACFOA3_000249"
     CDS             complement(3792..4694)
                     /locus_tag="ACFOA3_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6897325.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            complement(4843..5559)
                     /locus_tag="ACFOA3_000250"
     CDS             complement(4843..5559)
                     /locus_tag="ACFOA3_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6897326.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6128..6550)
                     /locus_tag="ACFOA3_000251"
     CDS             complement(6128..6550)
                     /locus_tag="ACFOA3_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6897327.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6668..7339)
                     /locus_tag="ACFOA3_000252"
     CDS             complement(6668..7339)
                     /locus_tag="ACFOA3_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6897328.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(7355..7981)
                     /gene="purN"
                     /locus_tag="ACFOA3_000253"
     CDS             complement(7355..7981)
                     /gene="purN"
                     /locus_tag="ACFOA3_000253"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6897329.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDRLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8088..8906)
                     /locus_tag="ACFOA3_000254"
     CDS             complement(8088..8906)
                     /locus_tag="ACFOA3_000254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6897330.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGTVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9165..9605)
                     /locus_tag="ACFOA3_000255"
     CDS             complement(9165..9605)
                     /locus_tag="ACFOA3_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6897331.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSETSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(9668..11074)
                     /locus_tag="ACFOA3_000256"
     CDS             complement(9668..11074)
                     /locus_tag="ACFOA3_000256"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6897332.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTDNGLQAIAVCRLKSTDRAALNKVAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            11245..12360
                     /gene="lptF"
                     /locus_tag="ACFOA3_000257"
     CDS             11245..12360
                     /gene="lptF"
                     /locus_tag="ACFOA3_000257"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6897333.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            12357..13427
                     /gene="lptG"
                     /locus_tag="ACFOA3_000258"
     CDS             12357..13427
                     /gene="lptG"
                     /locus_tag="ACFOA3_000258"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6897334.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13727..16312
                     /gene="acnB"
                     /locus_tag="ACFOA3_000259"
     CDS             13727..16312
                     /gene="acnB"
                     /locus_tag="ACFOA3_000259"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6897335.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16454..17449
                     /locus_tag="ACFOA3_000260"
     CDS             16454..17449
                     /locus_tag="ACFOA3_000260"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6897336.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(17672..18685)
                     /gene="ilvC"
                     /locus_tag="ACFOA3_000261"
     CDS             complement(17672..18685)
                     /gene="ilvC"
                     /locus_tag="ACFOA3_000261"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6897337.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(18765..19058)
                     /locus_tag="ACFOA3_000262"
     CDS             complement(18765..19058)
                     /locus_tag="ACFOA3_000262"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6897338.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(19125..19616)
                     /gene="ilvN"
                     /locus_tag="ACFOA3_000263"
     CDS             complement(19125..19616)
                     /gene="ilvN"
                     /locus_tag="ACFOA3_000263"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6897339.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(19627..21354)
                     /gene="ilvB"
                     /locus_tag="ACFOA3_000264"
     CDS             complement(19627..21354)
                     /gene="ilvB"
                     /locus_tag="ACFOA3_000264"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6897340.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            complement(22064..22148)
                     /locus_tag="ACFOA3_000265"
     tRNA            complement(22064..22148)
                     /locus_tag="ACFOA3_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22112..22114),aa:Leu,seq:caa)
     gene            22425..23078
                     /locus_tag="ACFOA3_000266"
     CDS             22425..23078
                     /locus_tag="ACFOA3_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6897341.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            23157..23810
                     /gene="hisG"
                     /locus_tag="ACFOA3_000267"
     CDS             23157..23810
                     /gene="hisG"
                     /locus_tag="ACFOA3_000267"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6897342.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            23914..24813
                     /locus_tag="ACFOA3_000268"
     CDS             23914..24813
                     /locus_tag="ACFOA3_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6897343.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            24810..26099
                     /gene="hisD"
                     /locus_tag="ACFOA3_000269"
     CDS             24810..26099
                     /gene="hisD"
                     /locus_tag="ACFOA3_000269"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6897344.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAALRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            26145..27242
                     /gene="hisC"
                     /locus_tag="ACFOA3_000270"
     CDS             26145..27242
                     /gene="hisC"
                     /locus_tag="ACFOA3_000270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6897345.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            27239..28156
                     /gene="hisB"
                     /locus_tag="ACFOA3_000271"
     CDS             27239..28156
                     /gene="hisB"
                     /locus_tag="ACFOA3_000271"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6897346.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            28251..29120
                     /locus_tag="ACFOA3_000272"
     CDS             28251..29120
                     /locus_tag="ACFOA3_000272"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6897347.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            29398..29829
                     /locus_tag="ACFOA3_000273"
     CDS             29398..29829
                     /locus_tag="ACFOA3_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC6897348.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            29839..30708
                     /locus_tag="ACFOA3_000274"
     CDS             29839..30708
                     /locus_tag="ACFOA3_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6897349.1"
                     /translation="MWKMLKHIAQTHRKRLLGTFSLVGLENLWMLVLYPVFGGRAINA
                     VIAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSA
                     VTARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLL
                     PRFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCV
                     GTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQ
                     RIGGLERNIKAGT"
     gene            complement(30759..31811)
                     /gene="sohB"
                     /locus_tag="ACFOA3_000275"
     CDS             complement(30759..31811)
                     /gene="sohB"
                     /locus_tag="ACFOA3_000275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6897350.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(31915..32004)
                     /locus_tag="ACFOA3_000276"
     tRNA            complement(31915..32004)
                     /locus_tag="ACFOA3_000276"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31968..31970),aa:Leu,seq:taa)
     gene            complement(32107..32180)
                     /locus_tag="ACFOA3_000277"
     tRNA            complement(32107..32180)
                     /locus_tag="ACFOA3_000277"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32146..32148),aa:Cys,seq:gca)
     gene            complement(32206..32281)
                     /locus_tag="ACFOA3_000278"
     tRNA            complement(32206..32281)
                     /locus_tag="ACFOA3_000278"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32246..32248),aa:Gly,seq:gcc)
     gene            complement(32303..32378)
                     /locus_tag="ACFOA3_000279"
     tRNA            complement(32303..32378)
                     /locus_tag="ACFOA3_000279"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32343..32345),aa:Gly,seq:gcc)
     gene            complement(32402..32477)
                     /locus_tag="ACFOA3_000280"
     tRNA            complement(32402..32477)
                     /locus_tag="ACFOA3_000280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32442..32444),aa:Gly,seq:gcc)
     gene            complement(32503..32578)
                     /locus_tag="ACFOA3_000281"
     tRNA            complement(32503..32578)
                     /locus_tag="ACFOA3_000281"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32543..32545),aa:Gly,seq:gcc)
     gene            complement(32658..33221)
                     /gene="pgsA"
                     /locus_tag="ACFOA3_000282"
     CDS             complement(32658..33221)
                     /gene="pgsA"
                     /locus_tag="ACFOA3_000282"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6897351.1"
                     /translation="MPWNLPIFLTWLRVLLIPVLTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(33327..33750)
                     /locus_tag="ACFOA3_000283"
                     /pseudo
     CDS             complement(33327..33750)
                     /locus_tag="ACFOA3_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8136 a         9284 c         8501 g         7866 t
ORIGIN      
        1 cgtctgaaaa cggggcggcg gtgtgtccgt acggcattaa gcgtatctct gccggctgag
       61 ggaaaaccct gcctgcccga tcaaaccaaa cggttgtgaa gcaaaagcct ttcaaacggc
      121 atcggtttaa cgtaccgacc acgcggcaac ggcatcggca aacattgccg ccacgtcgaa
      181 acctttttgt ttcataattt cttggaatcc ggtcgggctg gttacgttga cttcggtcag
      241 gttgctgccg ataacgtcca aaccggccag caggatgccg cgccgtttga gttcgggggc
      301 gagcgtttcg gcaatttcgc ggtcgcgtcc gcccaattcc tgcgccacgc cgcgcccgcc
      361 tgccgcaagg ttgccgcgtg tttcgccgtt ttgcgggata cgcgccaaag cataggggac
      421 gacttcgccg ccgataatca ggatgcgttt gtcaccgtgt acgatttcgg gaatgtagcg
      481 ttgcgccata atggtgcggg aatcaagctg catcagggtt tcgaggatgc tgccgatgtt
      541 ggggtctttt tcggtcaggc ggaagatgcc cataccgccc atgccgtcga gcggtttaat
      601 gatgatgtcg ccgtgttctt tcaaaaatgc gcggacatcg gcggaacggg tcgttaccag
      661 cgtgggcgcg gtaaagcggc tgaagttcaa aatcgccagt ttttcattga agtcacgcat
      721 cgactgtccg ctgttaaaga ctttcgcgcc ctgctgttcc gccagcgtca gtaattgggt
      781 ggcgtagagg tattgcatat cgaacggcgg atcggtacgc ataatcacgg catcaaatgc
      841 tttcaatgcc gtctgaactt tgtcggcggc ggcaaaccag tttttgtcgt gatcgtcttg
      901 tgcgccgaga aactcaaaag cagacgcatc agcaaccacc agaccgtttt taaccgacaa
      961 ttccccgctc aaggtatgaa acagccgcca accgcgtttt gccatctcgc gcatcatggc
     1021 gtaggtggtg tctttgtagg ttttgaaact tgccatcggg tcggcgataa agaggacttt
     1081 catcatattt cctttccggt gtgccgaatg tgccgcattt cgcgggcaaa ggggaaatgc
     1141 cgtccgaaca atattcagac ggcaaggatg gggttttact taggctgcca agaatctttc
     1201 agcgtgaccg tgcggttaaa caccggcgtg tctttgccgt ggtcttggcg gtcggttacg
     1261 aagtagccga tacgctcgaa ctgccaacgg ctttctgtcg gcaaatcttt cgcggcaggt
     1321 tcggcgtagg cggtgatttc cttgacggat tccggattga ggaaatcggt gaacggcagg
     1381 tattcgccgt cttcgccgcg cacggcatcg ggacgctcga cggtaaagag gcggtcgtac
     1441 agacggactt tgatttcggc ggcgtgttcg gcggaaaccc aatgaatcac gcctttaact
     1501 ttacggcctt ctggattttt gcccaaggtg tcgtggtcga tgctgcattt gagttcaagc
     1561 acattgcctg cttcgtcttt gacgacttca tcgcacttga tgacatagcc gtggcgcaag
     1621 cgtacttcgc cgccgggaat caggcgtttg aagcctttgg gcggattttc ggcaaagtcg
     1681 tcggcttcga tgtaaatggt ttgtgaaaca ggtacttcgc gatcgcccat ttcctcgtgg
     1741 ttcggatgga acgcggcacg gcggctttgg gttctgccgg tttcaaagtt ggtcagggtc
     1801 actttgagcg ggttcaacac cgccatcagg cgtggggcgg aattttccaa ctcttcgcga
     1861 atcgcgcctt ccaacacgct catatcgacg atgttttcag atttggaaat accggcgcgt
     1921 ttggcaaaca ggcgcagccc ttcgggcgtg tagccgcgtc ggcgcatacc ggaaatggtc
     1981 ggcatacgcg gatcgtccca gccggaaacg tgtttttcca caaccaactg attcaatttc
     2041 cgtttggagg taatggtgta caaaagctcc aaacgggaaa actcgtattg gcgcggacgg
     2101 gtggcatgcg gcgcaggaat gttgtccaac acccagtcgt acagcggacg gtgtgcttcg
     2161 aattcgagcg tacacaagga atgcgtgatg ccttcgatgg catcggagat gcaatgcgtg
     2221 tagtcgtaca tcgggtagat acaccatttg tcgccggtgt tgtggtgatg ggcgcggcgg
     2281 atgcggtaga tgacggggtc gcgcatattg atgttgcccg atgccatgtc gattttcagg
     2341 cgcagggttt tgctgccgtc ggggaactcg ccgtttttca tgcgtgtgaa caggtcgagg
     2401 ttttcttcga cgctgcggtc gcggtaaggg ctgtttttac ccgcttcggt cagcgtaccg
     2461 cggtattcgc gcatttcttc tggcgtcaaa tcatcgacat acgctttgcc gtctttaacc
     2521 aaaccgacgg cgtagtcata aagctggtcg aaatagttgg aagcgaaacg cggctcgccc
     2581 gcccaatgga aaccgagcca ctcaacgtct tctttgatgg cgttgacgta ttcgtcgttt
     2641 tctttttcgg ggttggtatc gtcaaaacgc aggttgcaca agccgtcgta aatatacgcc
     2701 aaaccgaagt tcaggcagat ggatttggcg tgtccgatgt gcaggtagcc gttgggctcc
     2761 ggcggaaaac gggtttggat ggtttcgtgt ttgccgcttt tgaggtcttc ttcgatgaag
     2821 gtgcggataa aatggttgtc cgcaaattgg tctttattga gcatagtttt ctttgaacag
     2881 atggcttcag acggcattgg ggcggttcct tatgccgtct gaagcggttt gatgatgtgt
     2941 ttattgtacc cgacttgcgc gctttgacat agcgttcaga cggcatcggc aatcaggcat
     3001 tccacccccg cctctttcag catcttctgc atcgcggtat cgggcagccg gtcggtaaat
     3061 actttgtcaa acgccgtaat gtcgccaagc ctgaccagcg cgttgctgcg gaatttgctg
     3121 tgatccacgc cgaggaagcg gacgcgcgcg ttggcaatca tcgcctgcat cacgctgact
     3181 tctttgtagt cgtcgtccaa aagcgaaccg tcgctttcca cgccgtgcgt actcatcacg
     3241 gcataatcga ctttgaactg gttgataaaa tcgacggttg ccacgccggt aataccgccg
     3301 tccaaaggac ggacgactcc agaagtgata atgaccgtat aatccgtccg cgccgaagca
     3361 atcgaggcgg cgtggatatt gttggtaatc accctcaggc tgccgcgccg cctgaccagc
     3421 tccgacacca cggcctccat cgtcgtgccg atactgacaa acagcgacga accgtcgggg
     3481 atgtgttccg caatcagccg ggcaatggcg tttttttcgt tttgacaccg ggtttggcgg
     3541 tcggcgggca ggccctccgg caagtttccg cccgaagatg cgccgccgtg atggcgtttc
     3601 aggctgccgg cctcctccaa ctcgcggatg tcgcggcgta tcgtctgcgg ggtaacgtcc
     3661 aatgcggcgg caagctcgtc caccgacata aactgatgcc ggcggacaag gcttaaaatc
     3721 tctccgtgcc tttggatttt cggtttcatc gttttctgcc tccttgcatc gggatgccga
     3781 ttttaccgtg ttcagcccag cgtggaaaac accaccatca gaaacggggc ggcaatattg
     3841 accaccacgc cgaagctgac cgctaccggc acgacttcca aaccgcccgc accctgaatc
     3901 acgggcaatg taaaatccat actggtcgca ccgccaaccc ccaccgccgc atccggaaaa
     3961 cgcttcatca gcagcgggat aaatgccagt gcaaacagct ctcgtgccaa atcgttcagc
     4021 agcatgatgc tgccccatac cgcgccgtaa gcctcggtca tgaccaaacc cgagagggaa
     4081 taccaaccga agccggaagc catcgccaaa cctttcgtcc acgacacacc gtctgtcgat
     4141 gcggcaaaca gcagcccgcc cgaaagagat gaaagcataa accagaccga caaccgaata
     4201 cccctgcggt tgaccaaaac ctgccgcaac gatacgccgc tgcttttgag ctgtacgccg
     4261 atgaggaaca ccagcagcat cagacaatac atgcccgcgc tttcagacgg catccaaata
     4321 tcgcgcatca gtttgccgaa tgcaaatccg agcagcacgc atccgagctg ccccacactg
     4381 cccgacacgc cgaccgaaac gcccttccct ttccccttta tccgccacgg gaataacttt
     4441 cccaacactg ccaaagcaag caggttcgcc ccgaccgtac aaacaaacag ccacagaacc
     4501 gtcaacgcca tatcgtccaa ccgcgaaccc aaatcctcca cgcgcgacaa cgatacgccg
     4561 atcagcagca gcacggcata caccaaaacc gacagcacct tgtccaaagc gggcaggtaa
     4621 ggcttgggca cacggataaa aaatccggcg aacatcggta ccaataccga aagcaatgtc
     4681 atcaggctgt ccatctcctg acctccttta ttgccgcatg atatatgcgg tttaaaaatt
     4741 gccgtctgaa aattgcagat acccgcatcc atatttcaga cggcatcagg ttcgccatta
     4801 aaaaaccgcc tgaaggttca ggcggcttat ccgctccggc attcaatctt ccaaagtctt
     4861 ttccaagcgt tccatacagt tgcccaaatg gcggcgcagg attttgacca cgcggttgcg
     4921 cctgcccgcc agcagcaggt cgaggatttc gcggtgttcg gaatgcgtat gcgtattgat
     4981 ggcgtgtttt tcctcgcgat gcacgcccgc cacggcgaca atcagggaag accgcgcgca
     5041 cagcgtgttc ataatgtcga acagcacatc gttgcccacc aggcgcgcca gttcgacgtg
     5101 gaaggcattg gacaggcggt tccagccgac gcggtcgccc ctgccggaag cctcttcttc
     5161 gcgccttatc atcgcataaa gcggcttgag gcgcgtttcc aaatccggca aatctgcgag
     5221 gatattcaaa atcatcgtct ccatttcgat gcgcgcattg aacacatcct gcatttcttt
     5281 caaatcggga acgtggacga acgcgcccct gttgggttgc aaatcgacaa tcttgtcgtg
     5341 cgccaaaagc gacagcgcgc cgcggacggt gttgcgcgaa cacaccatct gacggcaaag
     5401 ttcggattcg gtcagctttt tgccgggcag cagcacctga tcggtaatgc cgtccaaaat
     5461 cagggcgtaa acacggaaca gctccgaatc gtgccgctct tcgagaatca gggaagacgt
     5521 ggtcggcgca tggataatgt cgtcgttttc aaagttcatg atgttttccg tatttttacg
     5581 ctttcaaatt ttttaagatg ttttaaggcg gctgtgtttc aaatcgtgtc agaggaatta
     5641 aagcattgca caaatttatt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     5701 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataattc
     5821 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
     5881 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
     5941 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
     6001 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
     6061 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
     6121 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
     6181 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
     6241 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
     6301 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
     6361 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
     6421 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
     6481 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
     6541 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
     6601 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacgcctgaa
     6661 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
     6721 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
     6781 agaaatacat taccgcatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     6841 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     6901 ttttgccgtt ggtgattttc agccccgggg ggagtttcgc gtcatttgcc gccaactgcc
     6961 aggcaagcgt gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     7021 tgccgtaagt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     7081 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     7141 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     7201 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     7261 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     7321 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcaggaaa cgggcggcat
     7381 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     7441 cggcaacggc tttcggatag agtttgtgtt cgacagccaa aacccgggcg gcaatatcgt
     7501 ctgccgtatc accatcaagt atcggcacaa tcccttgcga tacaatcggg ccgcaatcca
     7561 gctcggccgt aacaaagtga atcgtacagc ctgcaacgcg gcaacccgct tccaaagcgc
     7621 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtggatg ttcatcagcc
     7681 tgccttcgta atgggcgcaa aactcggggg tcagaatccg cataaaacct gccaaaacca
     7741 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagccggg
     7801 atgtaaagtt tttatgattc agtctatcgg tcgggatgcc gcgttcggcc gcccattgca
     7861 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatagcgg
     7921 cattgacgat tgcctgcatg ttgctgccgc gcccggaaat caggatgacg atgtttttca
     7981 taatggtgcg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
     8041 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     8101 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     8161 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     8221 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagttgt
     8281 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgt gccgccgttg
     8341 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     8401 atgtcgtctt tggtcggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     8461 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     8521 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     8581 aggaatttca tcatgacttc ctgagcctgc tcttcggtca ttttgatttc tttgccgtca
     8641 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcgcc ctgttccttc
     8701 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     8761 ctgccgatcg aagaggtgtc gccctgcgcg gaagaagcgg cggcaggttc ggatgcagat
     8821 gcgggggcgg cttctttttt gccgcaggcg gaaagtgcca aagcggcgga aagggtcagt
     8881 gcgctgattt tgaaaatggt gttcatgatg gatcttcgct gtcgataagg tcggaaaaac
     8941 gggattatag ccgagtttga atgtttcaac acacaggatg acacataaag cgtcaatcgt
     9001 gtgttgccct gttttggaag ggattgaacc ttccaaaata agttttgatt ctaccgcccc
     9061 gagggacaga tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat
     9121 caagcctgtt taaattttgt aaatgcggca tttcagacgg cattttatgc cttgccctcc
     9181 atgccgtgat gttcgatggc aaaaccgctt cggcggtagg cggtaaagcg ttcgcgcgcg
     9241 tcggcgagat cttccaagct gttgccgacg atttccaaaa cgcgcgcggg caagaccgga
     9301 gcggtgttcc aaaaaccgtc ggacaggttc aaaacggtca tgccttcggg aattcggggc
     9361 agattgccgc cgcaggcaag caggacggat gtttcagacg gcatggcttc ttccgtttcc
     9421 caaatttcgt gcggaatgaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc
     9481 tgaagctgcc cgaacgaatc ggaccacacc agtatcctgc cgccgtcccg aatcgcacgg
     9541 gcaatcaggc ggcaggtgaa aatcggaact tgggcaacgt gcgtgtaaaa ggtggctttc
     9601 ggcatattgt ttgaacattt ggcaggataa tgccgtctga aaggcttcag acggcattgt
     9661 ggcaaaatta aagattccgc agatagttca gcagcaaggg aacgggacgg ccggtcgcac
     9721 ctttttccgc accagatttc cacgccgtac ccgcgatgtc aaggtgtgcc catggatagt
     9781 cttcggtaaa gtaggatagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga
     9841 tgtttggaat gtcggcaaaa ttggacttga gctggtcttt gtaggtttca aagagcggca
     9901 gttgccacgc tttgtcgtcc acgttgcggg aggcggcgag caggctgtcg atcaaatcct
     9961 gattgttgcc catcacgccg ctgacatcgt gccccaaggc gacgatgcat gcaccggtca
    10021 gggtggcgac gtcgatgacg gctttgggtt tgaactgttc ggcgtaagtg agtgcgtcgc
    10081 acaaaatcag acggccttcg gcatcggtgt tcaacacttc gatggtcagc cctttcatgc
    10141 tttttacgac gtcgcccggt ttgttggccg cgccggaagg catattttca caagtggcga
    10201 cgacggcaat caggttgatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga
    10261 cggttgccgc gccgcacata tcaaacttca tttcgtccat gttcaggccg ggtttgaggg
    10321 agatgccgcc ggtgtcgaag gtaatgccct tgccgaccaa taccacaggc gcggcttctt
    10381 tgtcggctgc accgaagtag ctcagttcga ccaaataggg atcttcgaca ctgcctttgg
    10441 cgaccgacca aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt
    10501 gcgcgcccag tttttcggct tcggctttgg cggtgcgcgc taaaaattcg ggcgtgcatt
    10561 cgttcggcgc agcattgccc aagtcgcggc agaggctttg tccgtaaacc tgtgcttcgg
    10621 cgacgcgcaa ggcttcttta acggcggctt cgtgtgcgct gtggaacacg gcggtttcaa
    10681 atttggcggg cttggcttct tttttgtagc ggtcgaaacg gtaggcggca ttgccgaacg
    10741 caatcgcaaa cgcttcggct acggcggcgg cttgcgcttc atcgaaggca tggatgtcca
    10801 cattgaccgt ttcctgattt tgcgcccatt tggcggcttc ggctgcgact ttgttcagcg
    10861 cggcgcggtc ggtgcttttc agacggcata cggcaatggc ttgcaaaccg ttgtcagtcg
    10921 ggatttttgt gtcggcaaaa ttttgacctt cttcaagcga agacaaaagg gcaagggcgg
    10981 tcggatggtt taattgcgcc tcttcggtgc agacaaataa ctgtgcgcct gcctgctgtt
    11041 cctgcaagat ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg
    11101 tcggtagttt tcggacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg
    11161 taccccattt gaagcaccgt ctgaaacctt gcgcggacaa tccgcctgcg ccgaaccgct
    11221 taccgccccc ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct
    11281 tttaccgccg tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc
    11341 aacctgctcg gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc
    11401 ggcttctggg tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg
    11461 ttgaccgtgt tgacccgcta ctggcgcgac agcgaaatgt cggtctggct atcctgcgga
    11521 ttggcattga aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg
    11581 gttgccgtca tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct
    11641 gaaatcctga agcagaagca ggaattgtct ttggtggagg caggcgagtt caacagtttg
    11701 ggcaagcgca acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg
    11761 aaaaacctgt tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa
    11821 gaaggtaact tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt
    11881 tacagcggca cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcaacctg
    11941 attatcagca ccacgcccaa actcatcgac cccgtttccc accgccgtac cattccgacc
    12001 gcccaactga ttggcagcag caacccgcaa catcaggcgg aattgatgtg gcgcatctcg
    12061 ctgaccgtca gcgttttgtt gttgtgtctg cttgccgtgc cgctttccta tttcaacccg
    12121 cgcagcggac atacctacaa tatcttgatt gccatcggtt tgtttttaat ttaccaaaac
    12181 gggctgaccc tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg
    12241 ctgcctatgc acattatcat gtttgccgtt gcactcatcc tgttgcgcgt ccgcagtatg
    12301 cccagccagc ccttctggca ggcggttggc aaaagtctga cattgaaagg cggaaaatga
    12361 acctgatttc acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg
    12421 ccttcctcgc tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag
    12481 gcagttacgg catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct
    12541 acgaactgat tcccctcgcc gtccttatcg gcggactggt ctccctcagc cagcttgccg
    12601 ccggcagcga actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga
    12661 ttctgtcgca gttcggtttt atttttgcta ttgccaccgt cgcgctcggc gaatgggttg
    12721 cgcccacact gagccaaaaa gccgaaaaca tcaaagccgc cgccatcaac ggcaaaatca
    12781 gcaccggcaa taccggcctt tggctgaaag aaaaaaacag cattatcaat gtgcgcgaaa
    12841 tgttgcccga ccatacgctt ttgggcatca aaatttgggc gcgcaacgat aaaaacgaat
    12901 tggcagaggc agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga
    12961 aaaacatccg ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag
    13021 aaaactggcc gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc
    13081 aaatgtccgt cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca
    13141 cccgaatcta cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga
    13201 tggcgctcgt cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa
    13261 aactcttcgg cggcatctgt ctcggattgc tgttccacct tgccggacgg ctcttcgggt
    13321 ttaccagcca actctacggc atcccgccct tcctcgccgg cgcactacct accatagcct
    13381 tcgccttgct cgccgtttgg ctgatacgca aacaggaaaa acgctaaacc aatgccgtct
    13441 gccccccttc ggacggcatt ggtttttatt atcatattcc cgtagacaga tagccgttcc
    13501 ctattatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactattgta
    13561 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaaataca ttacctgtca taacagttcc
    13621 atttttgtta aaactagtct atgatagcgg tacaaatatt gtttacaata tttaacgcaa
    13681 atcatttgca acccgacaaa agaaaaacag aaaaaggaac aaagagatgt tagaagccta
    13741 tcgtaaagcc gccgccgagc gcgccgccct tggtattccc gcccttcctc tgactgctca
    13801 gcaaaccgcc gatttggttg agctgctgaa aagcccgccc gcaggcgaag gcgagttctt
    13861 ggtcgaactg cttgcccacc gtgttccgcc cggtgtggac gatgccgcca aagtcaaagc
    13921 ctcattcctg gctgccgttg ccgaaggcag cgcgtccagc ccgctgatct cccccgaata
    13981 tgcgaccgaa ctcttaggta cgatgctcgg cggttacaat atccacgcct taatcgaact
    14041 cttggacgac gacaaactcg cgcccattgc cgccaaaggc ttgaaacata cgcttctgat
    14101 gttcgattcc ttccacgacg ttcaggaaaa agccgaaaaa ggcaacaaat acgcgcaaga
    14161 agttttgcaa tcttgggcag atgccgaatg gttcgcctca cgcgccaaag ttcccgaaaa
    14221 aattaccgtt accgttttca aagttgacgg cgaaaccaat acagacgacc tctcccccgc
    14281 gcccgacgcg tggagtcgtc ccgatattcc gctgcacgcg ctggccatgc tgaaaaaccc
    14341 gcgcgacggc atcacgcccg acaaaccggg cgaagtcggt ccgattaaat tgttggaaga
    14401 actcaaagcc aaaggccatc cggttgctta cgtcggcgac gtggtcggta ctggttcttc
    14461 acgcaaatcc gcgaccaact ccgtcatttg gcataccggc gaagacattc cgttcgtgcc
    14521 gaacaaacgc ttcggcggcg tatgtttggg cggcaaaatc gcgccgattt tcttcaatac
    14581 ccaagaagat tccggcgcgc tgccgattga agtcgatgta tctgctctaa aaatgggtga
    14641 tgtcgtcgat atcctgcctt atgaaggcaa aatcgtgaaa aacggcgaga ctgttgccga
    14701 gtttgaattg aaatcacaag tattgctgga cgaagtgcaa gccggcggcc gtatcaacct
    14761 gattatcggc cgaggtctga ccgccaaagc gcgcgaagcc ctgaaactgc ctgcctctac
    14821 tgcattccgc ctgccgcaag cgcctgccga aagcaaagcc ggtttcacct tggcgcaaaa
    14881 aatggtcggc cgcgcctgcg gtctgcccga aggacaaggc gtgcgtccgg gtacttactg
    14941 cgaaccgcgt atgacgacgg tcggctcgca agacacgacc ggcccgatga cccgcgacga
    15001 gttgaaagac ttggcttgtt tgggcttctc cgccgatatg gtgatgcagt ctttctgcca
    15061 caccgccgcc tatccgaaac ctgtcgatgt aaaaacccat aaagaactgc ccgcctttat
    15121 ttccacccgt ggcggcgtgt cactgcgtcc gggcgacggc gtcatccact cgtggctcaa
    15181 ccgcctgctg ctgcccgata ccgtcggcac cggcggcgac agccataccc gtttccccat
    15241 cggtatttcc ttccccgccg gctccggctt ggttgccttt gccgccgcaa cgggcgtaat
    15301 gccgctcgat atgcctgagt ctgtattggt acgcttcagc ggcaagctgc aaccgggcgt
    15361 aaccctgcgc gatttggtga acgccatccc gctgtacgca attaaacaag gtttgctgac
    15421 cgttgccaaa gccggtaaga aaaacatctt ctctggccgc atcctcgaaa tcgaaggcct
    15481 gcccgatttg aaagtggaac aagcctttga attgaccgac gcatccgccg aacgctccgc
    15541 cgccggctgt accgtgaagc tcaacaaaga gccgattatc gagtacatga aatccaacgt
    15601 cgtgttgatg aaaaacatga ttgccaacgg ctatcaagac ccgcgcactt tggaacgccg
    15661 catcaaagct atggaaaaat ggctggcaaa tcccgagttg ctcgaagcgg ataaagatgc
    15721 cgaatacgcc gccgtgattg aaatcaacat ggacgacatc aaagagccga ttatcgcctg
    15781 cccgaacgac ccggacgacg tgtgcttcat gtccgaacgc tccggcacca aaatcgacga
    15841 agtattcatc ggttcgtgta tgaccaacat cggccacttc cgcgccgcct ccaaactttt
    15901 ggaaggcaag gcagacaccc ccgtccgcct gtggattgcg ccgccgacca aaatggacgc
    15961 gaaacaattg tccgacgaag gacactacgg cgtactcgga cgtgccggcg cgcgtatgga
    16021 aatgccgggt tgctccttat gtatgggtaa tcaggcgcaa gtacgcgaag gtgcgaccgt
    16081 tatgtccacc tccacccgca acttcccgaa ccgtttgggt aaaaacacct ttgtttacct
    16141 cggttcggcg gaattggcag cgatttgctc caaactgggt aaaatcccga ccgttgaaga
    16201 atatcaagcc aatatcggca tcatcaacga acagggcgat aaaatctacc gctatatgaa
    16261 cttcaacgaa atcgacagct acaacgaagt agccgagacc gtgaacgttt aatccccgtc
    16321 atccgtatga agtaagggat tgaccgcaat gccgtctgaa caaccttcag acggcattgc
    16381 aacattccgc taacccttct ttccgcaaac gctgcaaata cggcgttcac gcccccacat
    16441 aaaggaaacg acagtgaacc tgaaaaaccg ccattttctg aaacttttag acttcacgcc
    16501 ggaagaaatc accgcctacc tcgaccttgc cgccgaattg aaagccgcca aaaaagcagg
    16561 gcgcgagatt cagcggatga aagggaaaaa catcgccctg atttttgaaa aaacctctac
    16621 tcggacgcgc tgcgcgtttg aagtcgccgc gcgcgatcaa ggcgcgggag tgacttattt
    16681 agagccgtcc gccagccaaa tcgggcataa ggaaagcatc aaagacaccg cccgcgtgtt
    16741 gggcaggatg tacgatgcca tcgaatatcg cggtttcggt caggaagttg ttgaagaatt
    16801 ggcgaaatac gcgggcgtac ccgtgttcaa cgggctgacc aacgagttcc atcccacaca
    16861 aatgcttgcc gacgcactga ctatgcgcga acacagcggc aaacctttga accaaaccgc
    16921 gtttgcctac gtcggcgacg cgcgttacaa catgggcaat tccctgctga ttttaggggc
    16981 aaaattgggg atggacgtgc gtatcggcgc accgcaaagc ctgtggccgt ctgaaggcat
    17041 tattgccgcc gcacacgccg ccgccaaaga aaccggcgca aaaattaccc tgaccgaaaa
    17101 cgcgcatgaa gccgtgaaga atgttgattt tattcatacc gatgtgtggg tcagcatggg
    17161 cgagccgaaa gaagtctggc aggaacgcat cgatttgctg aaagattacc gcgttacgcc
    17221 cgaactgatg gcggcatcgg gcaatccgca agtcaaattc atgcactgcc tgcccgcctt
    17281 ccacaaccgc gaaaccaaag tcggcgaatg gatttacgaa accttcgggc tgaacggtgt
    17341 ggaagttaca gaagaaatat tcgaaagccc cgccagcatc gtgttcgatc aggcggaaaa
    17401 ccgtatgcac acgattaaag cggtaatggt cgcggctctg ggcgactgac agaactgtgc
    17461 ctgtttaaat tcatccgcaa cacagatacc gtctgaacac gatgttcaga cggtatccat
    17521 atatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta ctatttgtac
    17581 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa aactgcctac
    17641 acgatgtgta ggtagtcccg tttgaaaaca atcagttttt gtcttggtca accaatttgt
    17701 tggcagtaat ccaaggcatc atggcacgca gttgtgcgcc gactttttca acttggtggt
    17761 cggcattcag acggcggcgg gcagtcatag acgcatagtt gacattaccc tcttggataa
    17821 acatttttgc gtattcgccg gtttggatgc gtttcagggc attgcgcatg gcttctttgc
    17881 tggaagcatt gaccacttca gggccggtaa cgtattcgcc gtactccgca ttgttggaaa
    17941 tggagtagtt catattggca ataccgcctt cgaaaatcag gtcaacaatc agtttcattt
    18001 cgtgcagaca ttcgaagtaa gccatttcag gcgcgtaacc ggcttcggtc agggtttcaa
    18061 aacctgcttt aatcaactcg accacgccgc cgcacaatac ggcttgttcg ccaaacagat
    18121 cggtttcggt ttcttcgcgg aaagtggttt caatcacacc gcctttggtg ccgccgttgg
    18181 cagccgcata agacagggcg atgtctttgg ctttgccgga attgtcttgg taaacggcaa
    18241 tcagagaagg cacgccgccg ccgcgtttgt attcactgcg tacggtatga cccggacctt
    18301 tgggggcaac cataatcacg tccaagtcgg cacgcggaac gatttggttg tagtgcacgt
    18361 tgaagccgtg tgcaaatgcc agcgttgcgc cttctttcaa attggctgta acttcggcgt
    18421 gatagacggc aggcatggtt tcgtcaggca gcagcagcat aacgacatcg gcttctttgg
    18481 tcgcttcagc aacggttttg acgacatgac cggctgcttc ggcttttttc caagaagaac
    18541 cttggcgcag accaatcacc acgtttacac ccgaatcttt caggttggcg gcatgggcat
    18601 gaccttgcga accgtaaccg atgatggcaa cggttttgcc tttgattagg gacagatcgg
    18661 catctttatc gtaatagact tgcatttgat ttcctttaag gtaaatggtt gtcgaagcct
    18721 taaaatgttg agcggcttcg gacgggttaa acagagtgtg ccgcttaatc ggcaacttca
    18781 ttcatcaata cgatttccaa cgcttcggtt ttgccgtcga cggactggac gaaggcttgg
    18841 aaatgcgcgc tggcgttatg ttcgtcaata gctgcttgag atttccaatt ttccacgaaa
    18901 acaaaacggt tcggtttgcc gatttcctga tggagatcgt agctgatgtt gccctcttct
    18961 gcacggctgg ctttgaccag ttctttaaac tgtgctgcca gtgtttctgt gtattccggt
    19021 ttgacggtaa ccagtgcgac aattttaatg ttcgacataa atctctcctg ccgttcgttt
    19081 ttcagacggc attcaaatac cgtgccgtct gaaaggttac ggcgttaaat tttcaaaata
    19141 cgctcaccgc gaccgatgcc ggccgcgcct gtacgtacgg tttccaaaat ttgggcgcgt
    19201 ccgaccgttt ccaaaaagga atccagcttg tcggtcgagc cggtaatttc aatcgtatag
    19261 ctgcggtcgg ttacgtcgat gatgctgccc cggtagattt cggtcaaacg caaaaattcg
    19321 tcgcggtctt taccggcggc acggactttt accaacatca gttctcgttc gacaaaacgg
    19381 ctttcgttca aatcgaccac tttaatcact tcaatcaatt tattgagttg cttggtgatt
    19441 tgttcgataa cctgctcgtc gccgtgggta acgatggtca tacgggacag ggttttgtct
    19501 tcggtcggcg caaccgccaa agaatcgata ttgtaatcgc gtgcagagaa caaaccgacc
    19561 acgcggctca tcgcacctga ttcgttttca atcagaacag ataagatatg tcgcatttgt
    19621 ctctccttac gccttttcgt ccgcacgcat atgcggcgga agtaccattt cgtccaaacc
    19681 tttgccgttg ccgaccatgg gcatcacatt ctgtttctgg tcggtcagga agtcgataaa
    19741 caccagcctg tctttttggt tcaatgcttc caacaacgca ccttccacat cagacttctt
    19801 gtccacgcgg ataccgatat ggccgtatgc ctcggcaagt ttgacgaaat cgggcaaaga
    19861 atcgaaataa gtttccgact ctcggccgcc gtaatatatt tcctgccact ggcgtaccat
    19921 accgagataa ccgttgttca gcgtaatgac gttaaccgga atccgatatt ggaaacaggt
    19981 ggacaactct tggatgttca tctgaatcga gccgtcgccg gtaatacaga atacgtcttg
    20041 atccggggcg gcaagttttg caccaatcgc ataaggcaga cccacgccca tcgtacccaa
    20101 accgccggaa ttgagccatt ggcgcggacg ttcgaaggga taatattgag ccgcaaacat
    20161 ttgatgctgc cctacatccg atgtgatgat tgccgaattg ccggtaatct cggcaagctt
    20221 ctgaatcaca tattgtggct tgataatttc gctgccgttg tcaaaccaca agcaatcttg
    20281 ggaacgccat tcctctatgg ttttccacca tttgcccaaa gcatcttcag acggcatgaa
    20341 ctcttgtttt tgccacagcg caaccatctc agacaaaaca tttttcacgt cgccgacaat
    20401 cggaatgtcc accttcacgc gtttggcgat gctggaagga tcgacatcga tatggataac
    20461 cttcttcgcc ttctcgaaaa atttggacgg tacggaaacc acacggtcgt caaaacgcgc
    20521 acctacggca agaacgacat ccgcattctg catggcaagg tttgcctcgt aagtaccgtg
    20581 cataccgagc ataccgagga attggcggtc gccggaagga taagcgccca agcccatcag
    20641 cgtacccgtg cacggagcac ccgtcattcg gacaaatcgg gtcagctctt cagaagcatt
    20701 acccaacacc acgccgccgc caaaatagac gaccggacgt ttggcagatg ccaacatctg
    20761 cacggccttt ttaatctgac cgatatgccc ttgaacaacc ggttgatacg aacggataaa
    20821 aatgtcttcc tgaggatagc tgaatttcgc catcgcctgc gtaacatctt tcgggacatc
    20881 aaccaccacg ggccccggtc ggccgcttgc ggcaatttgg aacgcctttt taatggtttc
    20941 cgccaactca ttgatgtccg taaccaggaa attgtgtttg acgcacggac gggtaatacc
    21001 caccgtatca acttcttgga acgcatccgt accaatcagg gaattgccta cctgcccgct
    21061 gatgaccacc atcggaatcg aatccgtata ggcagtagca ataccggtca gtgcattggt
    21121 aacgcccggg ccggatgtaa ccaatgccac gcccacctta ccgctgacgc gcgcatacgc
    21181 atctgccgcg tgtactgccg cctgctcgtg acgggtcaga atgtgcttga atttattgag
    21241 ttggaaaagg gcatcgtaga tttcgataac cgcaccgccg ggataaccga aaacgtactc
    21301 gacaccttcg gctttgagac tctgcactat gatttgcgcg cctgataact gcataacgac
    21361 ctcttttata cggtttcaaa ccaataggga caaaccgctt tgccacagca cctgtaatgc
    21421 aattccacca agcagcgatt tagggtacgc gcattggggg aacacggcaa cagacggatt
    21481 atccaatcaa ttggaaagga acacagagtt tgtgaaaaag agtggaaacg ataacgcaaa
    21541 ccgacagttc aatcaagaaa aatctttcat cttttaatat tttttgaaag cagagaaatt
    21601 attgattgat tttaaaagaa taaaatcagg agtacctttt ttgaaagatg gaaattgttg
    21661 acagtttgtg taggaggggc agatgtgaaa aacccttctt cgatatcaag aattttaaaa
    21721 tttacagggt ttcatcccaa taaagactcg ggatattgat tgaacttgat tttatttttg
    21781 atatatcaaa aatattccca accatacttc ctgaaaatgg ctcattgcac cggactgtat
    21841 tggacggcat tgacagaacc aagagggcta acaacgactt aatatattga ttgtatagtg
    21901 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
    21961 cgaggcaacg ccgtactggt ttaaatttaa tccactatat ttagtcttat ctatttcatt
    22021 aaacagcaat agacaaaaaa aataaccgct ccaaaagcgg ttgtggtgcc cagggtcgga
    22081 ctcgaaccga cacaccttgc ggcgggggat tttgagtccc ctgcgtctac caatttcgcc
    22141 acctgggctg gtgaagaagt cgtcattata atggcttttg aaattctgta aacctttttt
    22201 ttgaaattat tttatctgtt tttattttat ttttgatttt aaatagaatt tttattattt
    22261 taatcttact gttctttccg ctccaaagat tctgtatgat tcggcaattc ctgccgtgca
    22321 gacaacgtaa aaaaatacta cattaaatct gccaaacgcg ttaagatgga aatattcaaa
    22381 ttccgtacga atcaggtttt gctatttatt cttgggagat tgtcatgttt tccgtaccgc
    22441 gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc cgcctgcaaa cctcaagaca
    22501 acagtgcggc gcaagccgct tcttcaagtg catccgcgtc agctgcggaa aatgcggcaa
    22561 agccgcaaac gcgcggtacg gatatgcgta aggaagacat cggcggcgat ttcacgctga
    22621 ccgacggcga aggcaagcct ttcagcctga gcgatttgaa aggcaaggtc gtgattctgt
    22681 ctttcggctt tacgcactgt cccgatgtct gcccgacaga gcttttgacg tacagcgaca
    22741 cgttgaagca gttgggcggg caggctaagg acgtgaaagt ggtgttcgtc agcatcgatc
    22801 cggaacgcga cacgcctgaa atcatcggca agtatgccaa acagttcaat ccggacttta
    22861 tcggtctgac ggcaacgggc gaccaaaacc tgccggtcat caagcagcaa taccgcgtgg
    22921 tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta tttggtcgac cactcttccg
    22981 gtgcgtatct catcgacaaa aacggtgagg ttgccatttt ctcgccttac ggaagcgagc
    23041 cggaaacgat tgctgccgat gtaaggaccc tgctctgata aaaccgtatg ccgtctgcac
    23101 cgtcggcgcc tgttcagacg gcattattgt ttcaaccgac aaaggacatc cacaccatgc
    23161 aggataatgc tttgaccatc gccttatcca aggggcgcat ttttgaggag acgctgccgc
    23221 tgcttgccgc tgccggcatt gttccgactg aagagcctga aaaatcgcgc aagctgatta
    23281 tcgggacgaa ccatgaaaac atccgccttg tcattgtccg cgcaaccgat gtgccgactt
    23341 atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa agacgtgctg atcgaacacg
    23401 gcggcacggg gctttaccgg cctttggatt tggagattgc caagtgccgc atgatggttg
    23461 ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc cggatgccgt ctgaagattg
    23521 ccacaaagta tcctgaaatc gcggcatccc attttgccgg caagggtgtc catgtggaca
    23581 ttatcaaact gtacggctcg atggaacttg cgccgctggt cggcttgagc gatgcgattg
    23641 tggacttggt ttcgacgggc aacaccttga aggcaaacgg cttggaagca gtcgaacaca
    23701 tcgtcgacat ttccagccgc ctggtggtca acaaggctgc tttgaaaacg aaatacgcgc
    23761 tgctggagcc gattattcag gcgttcggcg gcgcagtgaa ggcgaagtaa gcatccattt
    23821 gaataaagat gcgttttcag acgaccctat ccgttcccgc cgacaggtcg tctgaaaata
    23881 tcaccggcag taaactgtat aggagaagtt aaaatggttg caaaaataaa aaaattctca
    23941 gattcaaccc tttccgtttt gaataacggc gagcgtcggt tttatgtcta ttgtctgacc
    24001 gacctgaaaa aagacaaaat cctctacatc ggcaaaggct gcggtaatcg tatcttcgag
    24061 catgaatggg ttgctagtcg ttcacaagac ccagtctccg gcgagattat cgatcggaaa
    24121 ctcaaagcca tctccaaatg caagaaactc ggtcgctata tcatcagcta tcatctgact
    24181 gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt ttgttaaatc tgtcttgggt
    24241 aaaaaactca aaaataaaat tgccgggcat ggtccgggtg gtattagcgt agaagaacta
    24301 gatcgccgct ttggattctc ttctctccca cttaacgaga ttaaccccga cgggctgatt
    24361 ctcgccatca aaatccacaa tgctttcgat ttagatactg acgaagaatt agactacctt
    24421 ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt tgggcaactg ggttatcggt
    24481 aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc acaccggtct gcaaaacgct
    24541 gttgtcagtg catacgaagt ggacggtttt gaaacaatgg ttgaggaaac caaaaacggt
    24601 agaaaacaat cccgttaccg tttccgcact acctctcgta gcgaagaggt attagccaaa
    24661 ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg gtagcggggg agaaaaagcg
    24721 tatatcagac ccaaaacaga gacagaaact gaacaagaga atattcagac gacccccaat
    24781 ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact caacacccaa tcgcccgatt
    24841 tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc gcaaaacccc gaaaccgaac
    24901 gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg cgatgcggct ttgattgaat
    24961 acaccaacaa attcgatcag acaaacgcta aaagcatcga tgatttaata cttacgcaag
    25021 ccgatttgaa agcggcgttc gagcgcattc cgaacgacgt tcagacggca ttgcagaccg
    25081 ccgccctccg tgtcgaaagc taccaccaac gccaaaaaat ggaatcgtgg agctacaccg
    25141 atgaagacgg cacgctgttg ggacaacaga ttaccccgct tgaccgcgtc ggcatttacg
    25201 tccccggcgg caaggcggca tatccgagtt ccgtcatcat gaacgccatg cccgcccacg
    25261 tcgcaggcgt gaaagaaatt attatggtcg tgccgacacc gaaaggcgaa cgcaacgaca
    25321 tcgtgcttgc cgccgcatac gtcgccggcg taaccaaagt cttcaccgtc ggcggcgcgc
    25381 aggcggttgc cgccctcgcc tacggcacgg aaaccatccc ccaagtcgat aaaatcaccg
    25441 gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt gttcggcgtg gtcggcatcg
    25501 acatggtggc ggggccgtct gaaatcttgg tcatcgccga cggcacgaca cctgccgatt
    25561 gggtggcgat ggatttgttc agccaggccg aacacgacga aattgcccaa gccatcttaa
    25621 tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc tatggacagg ctcatcgaaa
    25681 ccatgccgcg ccgcgacatc atcgaagcct cgctcggcaa caggggcgcg atgatactcg
    25741 ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat ttcccccgaa cacttggaat
    25801 tgtcagtcga aaatccgcag gaatgggcga aaaaaatccg ccacgccggc gcgattttca
    25861 tgggacgcta caccggcgaa agcctcggcg actactgcgc cggcccaaac catgtgttgc
    25921 ccaccagccg aaccgcccgc ttttcctcgc ctttggggac atatgatttc caaaaacgct
    25981 ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt aggcgaaacc gccagcgtgc
    26041 tggcacacgg cgaaagcctg accgcccacg cccgcgcggc agagttccgt atgaaataat
    26101 gccgaaacgg cgtacaggca tattccaacc attaaggaaa cacgatgaaa tccgtccgct
    26161 ccttcatccg cgacgacata caagctatgt cggcatatca gattgccgac gttccgcccg
    26221 gctttgccaa actcgattcg atggaaagtc ccgtccaccc ttttgccgga catgaaacgc
    26281 tgttgcagga atggcaggca cggcttgccg ccgcgcccat ccatctttac cccaatccct
    26341 ccggcagcgg tttacaggaa gcattacgtt cggcgttcga cattcccgac tgcgccgaca
    26401 tcgcgctggg caacggctcg gacgagctga tacagttcat cacgatgctg accgccaaac
    26461 cgggcgcggc aatgttggca gccgaaccca gtttcgtcat gtaccgccac aacgccgcgc
    26521 tgtacggcat ggattatgtc ggcgttccac tgaacggaga tttcaccctc aacctgcccg
    26581 ccgtcctcga agccgtcagg aagcaccgcc ctgccctgac ctttatcgcc taccccaaca
    26641 accccaccgg cgtatgtttc acgcgtgccg aaatcgaagc cgtcatcgaa gcttcagacg
    26701 gcatcgtcgt cgtcgacgaa gcctacggcg cattcaacgg cgacagcttc ctgccgcagg
    26761 cgggcagcat tcccaacctc atcgtcatgc gtaccgtcag caaaatcggt tttgccggac
    26821 tgcgtatcgg ctatgcggca ggctgtcccg aagtcatcgg cgaactgcaa aaaatcctgc
    26881 cgccctacaa tatgaaccaa ctgagcctga ccaccgccaa actcgccctg caacactacg
    26941 gcatcatctc tgccaacatc gacagcctga aaaacgaacg cgaacggatg ttcgccgaat
    27001 tgggcaaaat atgccgtctg aacacctttc caagtcaggc aaacttcatt accatacgcg
    27061 tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa ccgcatcttg gttaaaaaac
    27121 tgcatggcgc gcacccgctt ttggaacact gcctgcgcat taccgtaggc agccccgcac
    27181 aaaacgatgc cgttctcaac atcattcgcc aactttactg ccaaccaacg gatttcctat
    27241 gaatttgact aaaacacaac gccaactgca caactttctg accctcgccc aagaagcagg
    27301 ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc cccgtcgcac tctacaaact
    27361 caaacaacgc cttgaaaagc aggcagaaga cccagatgca cgcggcatcc gtcccagcct
    27421 gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc tggctcgaca gaaaacaccg
    27481 cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga actgccgaaa cccaaattgc
    27541 cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc aaccgcaata cctgcgaaac
    27601 ccaaatcacc gtctccatca acctcgacgg cagcggcaaa agcaggctgg ataccggcgt
    27661 acccttcctc gaacacatga tcgaccaaat cgcccgccac ggcatgattg acatcgacat
    27721 cagctgcaaa ggcgacctgc acatcgacga ccaccacacc gccgaagaca tcggcatcac
    27781 actcggacaa gcaatccggc aggcactcgg cgacaaaaaa ggcatccgcc gttacggaca
    27841 ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc atcgaccttt ccggccgccc
    27901 cggactcgtg tacaacatcg aatttacccg cgcactaatc ggacgtttcg atgtcgattt
    27961 gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg atgaccctgc acatcgacaa
    28021 cctcagcggc aaaaacgccc accatcaggc ggaaaccgta ttcaaagcct tcgggcgcgc
    28081 cctgcgtatg gcagtcgaac acgacccgcg catggcagga cagaccccct cgaccaaagg
    28141 cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc tcaggctttt cagacggtat
    28201 cggaacagat aagattacac tacactacaa acagaaaagg agtaaacatc atgtccgcaa
    28261 acgaatacgc acaaatcggc tggataggct tagggcaaat gggtctgcct atggtaacgc
    28321 ggctcttgga cggcggcatc gaagtcggcg tatacaaccg ctcgcccgac aaaactgccc
    28381 ccatctccgc caaaggcgca aaagtttacg gcaacaccgc cgaactcgtc cgcgactatc
    28441 ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg cgacatcctg aacggagtcc
    28501 gcgacggatt ggccggcaaa atcatcgtca acatgagcac catctccccg accgaaaacc
    28561 tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt tgccgaagca cccgtttccg
    28621 gatcggtcgg gcccgccacc aacggcacgc tgctgattct gttcggcggc agcgaagccg
    28681 ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa aaaaaccttc catttcggcg
    28741 atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc gctcttgggc attttcggcg
    28801 aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg catcgatacc gacaccatcg
    28861 tcgaagccat cggcggctcg gcaatggact cgcccatgtt ccaaaccaaa aaatccctgt
    28921 gggcaaaccg cgaattcccg cccgccttcg ccctcaaaca cgcctccaaa gacctcaacc
    28981 tcgccgtcaa agagcttgaa caggcaggca acaccctgcc cgccgtcgaa accgttgctg
    29041 ccagctaccg caaagcagtc gaagccggct acggcgaaca ggacgtttcc ggcgtttacc
    29101 tgaaactggc agaacactga cttccctttc caaacaatat gccgtctgaa atattttcag
    29161 acggcatttt tatcgcccca cgcttaaaat cagtcccgat tatgactata tagtggatta
    29221 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
    29281 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
    29341 ctggtttttg ttaatccact ataatccgca caaatttagt caatatcaag accaattatg
    29401 aaccaactcg accaacttgg cacccgtatc aacctgattt gcaatgtctt cgacaaatgg
    29461 atcgggcagc aggatctgaa ttacaacctc tttgccgtac tttataccct ggcaaccgaa
    29521 ggcagccgca cgcaaaagca tatcggcgaa gagtggagtt tgcccaaaca gaccgtttca
    29581 ggcgtatgca aaacccttgc cggacaaggg ttgattgaat ggcaggaaag cgaacaggac
    29641 cggcgcgaac ggttgctgtc gctgaccgaa aaaggcaaag cccatgccgc acctttaaca
    29701 gaaagcgcgc aggaattcag cgacaaagta tttgccacat tcggcgacaa gcgcacaacc
    29761 cggctgtttg ccgatttgga tgcactggct gaagtgatgg aaaaaacaat ctcggaaaat
    29821 aaaaaatagg gagcaaatat gtggaaaatg ttgaaacaca tagcccaaac ccaccgcaag
    29881 cgtctgctcg gcacgttttc cctggtcgga ctggaaaacc tttggatgct ggtattatat
    29941 ccagtgtttg gcggccgggc gatcaatgcc gtgattgcgg gggaggtgtg gcaggcgttg
    30001 ctgtacgctt tggttgtgct tttgatgtgg ctggtcggtg cggcgcggcg gattgccgat
    30061 acgcgcacgt ttacgcggat ttataccgaa atcgccgtgc cggtcgtgtt ggaacagcgg
    30121 cagcgacaag tcccgcattc ggcggtaact gcgcgggttg ccctgtcgcg tgagtttgtc
    30181 agcttttttg aagaacacct gccgattgcc gcgacatccg tcgtatccat attcggcgcg
    30241 tgcatcatgc tgctggtgct ggaattttgg gtcggcgtgt cggcggtggg catacttgcg
    30301 ttgtttttat ggcttttgcc acgttttgcc gccatcagcg aaaacctgta tttccgcctg
    30361 aagaacagct tggaacgcga caaccacttt atccgaaaag gcgaccggcg gcagctgtac
    30421 cgccattacg gactgcttgc gcgcctgcgt gtgctgattt ccaaccgcga agccttcggc
    30481 tatctctgcg tcggcacggc gatgggtatt ttgttcggct ttgcttttgt gatgatgacg
    30541 ctcaaaggct acagcagcgc ggggcatgtc tattcggtcg gcacttatct gtggatgttt
    30601 gccatgagtt tggacgacgt gccgcgattg gtcgaacaat attccaattt gaaagacatc
    30661 ggacaacgga tagggggatt ggaacggaac atcaaagccg gaacttgaaa aatgccgtct
    30721 gaacacgctt cagacggcat ttccatccgt tcggcaaact acatcacatc cgcccgccgg
    30781 ttgacaagtt tggcaaacaa cttttcaaca gaagcttccg cctgcaaacc aatgcgctgg
    30841 atcaggcttt gcttctcctg atatttcact tcgataacct gtttgttttc aaacgctttc
    30901 aacaacaaat catcactggt cgaaatctcg tcaatcaagt tcaacgccaa cgcctgccga
    30961 ccgaaccaat gctcgcccgt tgccacttcc tcaatatcca attgagggcg gttctcgctg
    31021 acaaactgct tgaacaactg atgcgtttcc tccagttcct gtcggaattt ctgtttgccc
    31081 ttttccgtat tttcacccat aaaagtaacc gtgcgcttaa attcgcccgc cgtcatcaca
    31141 tccacatcaa tatcatgttt tttcaacagg cggtggatat tcggtacttc cgccaccaca
    31201 cccaccgaac cgacaatcgc aaacggagcg gaaacaattt tatccgccac acacgccatc
    31261 atataaccac cgctcgctgc caccttatcg acggcgacgg tcagcggaat attgcgttcg
    31321 cgcaaacgcc taagctgtga agccgccaaa ccgtaaccgt gaaccacgcc gcccggactt
    31381 tccaatctaa gcagaacctc atcttcaggc ttggcaatca aaagcaccgc cgtaatctca
    31441 tgacgcaagg attctacggc gtgtgcatac aaatcgccgt caaaatccaa cacaaaaagg
    31501 cgggattttt gcgtttcggc agatttctcc ccaccctcct tcaaacgctt tttctctgct
    31561 ttggcttccg ccttttcctt tttcttttcc tctttttcct gatgttttgc ctcttccccg
    31621 cttaaaaaaa atgcctcaaa cgattgccgc tgttttttat aattttccga aaaatccgtc
    31681 agtacgacac tgccgctttc cgactgtttc ttactctgta cgatagccaa cacaatcagc
    31741 gcaattgcgc cgaacacggt aagcagttcg agcaggaaaa taccgtaatt cagtaaaatt
    31801 tctttccaca ttgattggat ttcctcttgt tcaggcatga acatgtcaat attgtccatc
    31861 accgtccgac agataaaaaa ataaccgctt ggagcggtat tgtcattttc agcttggtgc
    31921 ccggagccgg aatcgaaccg gcacgggatg tttagtcccg acggatttta agtccgttgt
    31981 gtctacctat ttcaccaccc gggcatttgt gaaaggtgga ggcgggggcg cggattttaa
    32041 ccggcctata tgaagattgc actcctcata gcataaacac tctgccaccc cgccatagta
    32101 cgataatgga ggcgagagtc ggaatcgaac cggcgtagac ggatttgcaa tccgctgcat
    32161 aaccactttg ctatctcgcc ctaaaactgg cttatctaaa aaacttggag cgggaaacga
    32221 gtctcgaact cgcgacctca accttggcaa ggttgcgctc taccaactga gctattcccg
    32281 cgcgttcaaa catatcggtt tttggagcgg aaaacgagtc tcgaactcgc gacctcaacc
    32341 ttggcaaggt tgcgctctac caactgagct attcccgcgt tgatatgttt gaaataaaac
    32401 ttggagcggg aaacgagtct cgaactcgcg acctcaacct tggcaaggtt gcgctctacc
    32461 aactgagcta ttcccgcaat gattgtggga aaatgaaatt tttggagcgg gaaacgagtc
    32521 tcgaactcgc gacctcaacc ttggcaaggt tgcgctctac caactgagct attcccgccc
    32581 gatttcattc tccaacatcg aagagacgca attattatgg attctgtttt tgccgtcaag
    32641 ctatttttat gtttttttca ggcgatttct ttccacgcca ttttcagata atacagcatc
    32701 gaccagactg tcagcaaaga tgcgataaac atcaatatgt tgccgataac aacaagatta
    32761 aatccgtaaa aatcgggaat attcagcagc agcaggaaaa ttgccagcat ttgcgcggcg
    32821 gttttaaact taccgacggt ggcgacggca acgctgttcc ttttgcccat ttgcgccatc
    32881 cattcgcgca atgcggaaat ggtaatttcc ctgccgatga tgatcatggc aaacaaaaca
    32941 taggtccggt cgagtttgac cagtaaaagc aaagagacgg cgaccatcag cttgtcggca
    33001 acgggatcga ggaaggcgcc gaaatccgag gtctgtttcc acaaccttgc caaaaatccg
    33061 tcaaaccagt cggtcaaggc ggcaacggca aaaatgacgg cggcggtgag attaatcgtt
    33121 tcctccgcga accacggaaa aggcaggtaa aaaagggctg tcaggacagg aatgagcaag
    33181 accctcaacc atgtgaggaa gatggggaga ttccaaggca tcggttttct ctgtgcagac
    33241 tgtaaagttg tgattataac ggttatcctc ataacccaaa acgtaaaatt gctgcatggg
    33301 cattcccccg ccccgccaat ctgttttcac attcttttca aacgcaggaa aatggcgggc
    33361 aataaaagca aaatacccag tttcaggctg aaaacggcag gttgtgccaa cacttcgaca
    33421 aggcggtctt ccgtgcgggc aaaatcttta ttgcttatag acactgccac tgttgcggta
    33481 ttccaacaga acgccgttta aaaaaccttt gccgactgtt tcgcttaaaa cggctctaac
    33541 ctgctccgcc ctgatggttc tgccgatatt gccgcctgtg cacaaactgt cgaacccata
    33601 gcaggaaagc cggtaatgct gcccgtctgc atccagtttg attgcccgtc cgctgcggtt
    33661 gggggcggta acggtcaatt ccgcatattc taatgttttt tcttgttcgt gaaatgccgt
    33721 caggtaaggt gcaataaaaa cggcggacaa cagcagacag cttatggcgg caaaccatac
    33781 ccagcga
//
LOCUS       DAXIEB010000007        32676 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000007 DAXIEB010000000
VERSION     DAXIEB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.007"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..932
                     /gene="mafB"
                     /locus_tag="ACFOA3_000284"
     CDS             <1..932
                     /gene="mafB"
                     /locus_tag="ACFOA3_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6897352.1"
                     /translation="SDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAA
                     GALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEKN
                     TREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFSAAYNTRTT
                     RKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLF
                     PGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETR
                     DGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            947..1177
                     /locus_tag="ACFOA3_000285"
     CDS             947..1177
                     /locus_tag="ACFOA3_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6897353.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1326..2063
                     /locus_tag="ACFOA3_000286"
     CDS             1326..2063
                     /locus_tag="ACFOA3_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC6897354.1"
                     /translation="MVKTADGYKAIAHIRAGESVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSENGRTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            2060..2479
                     /locus_tag="ACFOA3_000287"
     CDS             2060..2479
                     /locus_tag="ACFOA3_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC6897355.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            2613..3353
                     /locus_tag="ACFOA3_000288"
                     /pseudo
     CDS             2613..3353
                     /locus_tag="ACFOA3_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            3356..3679
                     /locus_tag="ACFOA3_000289"
     CDS             3356..3679
                     /locus_tag="ACFOA3_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6897356.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            3738..3965
                     /locus_tag="ACFOA3_000290"
     CDS             3738..3965
                     /locus_tag="ACFOA3_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897357.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            4092..4634
                     /locus_tag="ACFOA3_000291"
     CDS             4092..4634
                     /locus_tag="ACFOA3_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6897358.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LELSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            4769..5185
                     /locus_tag="ACFOA3_000292"
                     /pseudo
     CDS             4769..5185
                     /locus_tag="ACFOA3_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            5246..5476
                     /locus_tag="ACFOA3_000293"
     CDS             5246..5476
                     /locus_tag="ACFOA3_000293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897359.1"
                     /translation="MKSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQT
                     FNNGSAEYHGHVRSWQDLHTDQKNALKKAGLD"
     gene            5502..5831
                     /locus_tag="ACFOA3_000294"
     CDS             5502..5831
                     /locus_tag="ACFOA3_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897360.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            6248..6649
                     /locus_tag="ACFOA3_000295"
     CDS             6248..6649
                     /locus_tag="ACFOA3_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897361.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            6831..7220
                     /locus_tag="ACFOA3_000296"
     CDS             6831..7220
                     /locus_tag="ACFOA3_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897362.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(7261..8718)
                     /locus_tag="ACFOA3_000297"
     CDS             complement(7261..8718)
                     /locus_tag="ACFOA3_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6897363.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPASNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(8733..9563)
                     /locus_tag="ACFOA3_000298"
     CDS             complement(8733..9563)
                     /locus_tag="ACFOA3_000298"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6897364.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(9641..10030)
                     /locus_tag="ACFOA3_000299"
     CDS             complement(9641..10030)
                     /locus_tag="ACFOA3_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6897365.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(10166..10690)
                     /gene="nspA"
                     /locus_tag="ACFOA3_000300"
     CDS             complement(10166..10690)
                     /gene="nspA"
                     /locus_tag="ACFOA3_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6897366.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDNFSQTSIGLGVLAGVSYAVTPNVDLDTGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            complement(10797..11057)
                     /locus_tag="ACFOA3_000301"
     CDS             complement(10797..11057)
                     /locus_tag="ACFOA3_000301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897367.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            complement(11277..12452)
                     /gene="hemW"
                     /locus_tag="ACFOA3_000302"
     CDS             complement(11277..12452)
                     /gene="hemW"
                     /locus_tag="ACFOA3_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6897368.1"
                     /translation="MHAISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAITTGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAQNNKQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(12509..15034)
                     /gene="ligA"
                     /locus_tag="ACFOA3_000303"
     CDS             complement(12509..15034)
                     /gene="ligA"
                     /locus_tag="ACFOA3_000303"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6897369.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPEFKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QELGFSLPNGNFGCFKNIDEVLAFYGHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQTPSED
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPT
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSETQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQALIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(15182..16468)
                     /locus_tag="ACFOA3_000304"
     CDS             complement(15182..16468)
                     /locus_tag="ACFOA3_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6897370.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGQPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(16662..17234)
                     /gene="ampD"
                     /locus_tag="ACFOA3_000305"
     CDS             complement(16662..17234)
                     /gene="ampD"
                     /locus_tag="ACFOA3_000305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6897371.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRGGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRRYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            17318..18313
                     /gene="mltG"
                     /locus_tag="ACFOA3_000306"
     CDS             17318..18313
                     /gene="mltG"
                     /locus_tag="ACFOA3_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6897372.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            18373..18993
                     /gene="tmk"
                     /locus_tag="ACFOA3_000307"
     CDS             18373..18993
                     /gene="tmk"
                     /locus_tag="ACFOA3_000307"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6897373.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            19215..20495
                     /locus_tag="ACFOA3_000308"
     CDS             19215..20495
                     /locus_tag="ACFOA3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6897374.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            20731..20934
                     /locus_tag="ACFOA3_000309"
     CDS             20731..20934
                     /locus_tag="ACFOA3_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897375.1"
                     /translation="MKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDSFGQF
                     LFGCSAEATRTAPISVKRSCLFT"
     gene            21042..22091
                     /gene="lpxK"
                     /locus_tag="ACFOA3_000310"
     CDS             21042..22091
                     /gene="lpxK"
                     /locus_tag="ACFOA3_000310"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6897376.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEGAEGRLKARFG"
     gene            22281..22862
                     /locus_tag="ACFOA3_000311"
     CDS             22281..22862
                     /locus_tag="ACFOA3_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6897377.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            22931..23113
                     /locus_tag="ACFOA3_000312"
     CDS             22931..23113
                     /locus_tag="ACFOA3_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6897378.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            23110..23871
                     /gene="kdsB"
                     /locus_tag="ACFOA3_000313"
     CDS             23110..23871
                     /gene="kdsB"
                     /locus_tag="ACFOA3_000313"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6897379.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEAAAALKLPQHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            23894..24211
                     /locus_tag="ACFOA3_000314"
                     /pseudo
     CDS             23894..24211
                     /locus_tag="ACFOA3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            24313..24513
                     /locus_tag="ACFOA3_000315"
                     /pseudo
     CDS             24313..24513
                     /locus_tag="ACFOA3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            24670..24762
                     /locus_tag="ACFOA3_000316"
     tRNA            24670..24762
                     /locus_tag="ACFOA3_000316"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24704..24706,aa:Ser,seq:gct)
     gene            25188..25973
                     /gene="trpA"
                     /locus_tag="ACFOA3_000317"
     CDS             25188..25973
                     /gene="trpA"
                     /locus_tag="ACFOA3_000317"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6897380.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            26010..26882
                     /gene="accD"
                     /locus_tag="ACFOA3_000318"
     CDS             26010..26882
                     /gene="accD"
                     /locus_tag="ACFOA3_000318"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6897381.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(26942..27553)
                     /locus_tag="ACFOA3_000319"
     CDS             complement(26942..27553)
                     /locus_tag="ACFOA3_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6897382.1"
                     /translation="MRRTTLLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            27662..27886
                     /locus_tag="ACFOA3_000320"
     CDS             27662..27886
                     /locus_tag="ACFOA3_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6897383.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            27887..28033
                     /locus_tag="ACFOA3_000321"
     CDS             27887..28033
                     /locus_tag="ACFOA3_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897384.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            28249..29283
                     /gene="pyrC"
                     /locus_tag="ACFOA3_000322"
     CDS             28249..29283
                     /gene="pyrC"
                     /locus_tag="ACFOA3_000322"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6897385.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(29364..29789)
                     /gene="nusB"
                     /locus_tag="ACFOA3_000323"
     CDS             complement(29364..29789)
                     /gene="nusB"
                     /locus_tag="ACFOA3_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6897386.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(29867..30343)
                     /gene="ribH"
                     /locus_tag="ACFOA3_000324"
     CDS             complement(29867..30343)
                     /gene="ribH"
                     /locus_tag="ACFOA3_000324"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6897387.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(30391..30714)
                     /locus_tag="ACFOA3_000325"
     CDS             complement(30391..30714)
                     /locus_tag="ACFOA3_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6897388.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            30881..31600
                     /gene="rnc"
                     /locus_tag="ACFOA3_000326"
     CDS             30881..31600
                     /gene="rnc"
                     /locus_tag="ACFOA3_000326"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6897389.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEKKLPLKKKKK"
     gene            31647..32570
                     /gene="era"
                     /locus_tag="ACFOA3_000327"
     CDS             31647..32570
                     /gene="era"
                     /locus_tag="ACFOA3_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6897390.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         7972 a         7546 c         9215 g         7943 t
ORIGIN      
        1 tatccgacaa ctacaacaac ctcggcagca atttctccga ccgcgccgat gaagccaaca
       61 gaaaaatgtt cgagcacaat gccaagctcg accgctgggg caacagcatg gagtttatca
      121 acggcgtcgc cgccggcgcg cttaacccct ttatcagcgc gggcgaagcc ttgggcatag
      181 gcgacatact gtacggaacg cgctatgcca tagacaaagc cgcaatgcgc aacatcgccc
      241 ccttgcccgc cgagggcaaa ttcgccgcca tcggcggctt gggcagcgtg gcgggctttg
      301 aaaagaatac gcgcgaagcc gttgaccggt ggatacagga aaatcccaat gccgccgaaa
      361 ccgtcgaagc cgtcttcaac gttgccgctg ccgccaaagt cgcgaagctg gcaaaggcgg
      421 cgaaaccggg gaaagctgcg gttagcgggg atttttctgc tgcatacaat acaagaacaa
      481 ctagaaaagt tactacagaa acagaggggt taaatagaat cagacagaac cagaaaaata
      541 gtaatataca tgagaaaaat tatggaagag ataatcctaa tcatattaat gttttatctg
      601 gaaattctat acaacatata ctgtatggag atgaagcagg aggtgggcat ctttttcctg
      661 gcaaacctgg taagacaaca ttcccccaac attggtcagc cagtaaaata actcatgaaa
      721 ttagtgatat cgttacatcc ccaaaaacgc aatggtatgc acagactgga acaggcggca
      781 aatatattgc taaaggaaga ccagccaggt gggtatcata tgaaacgaga gatggaattc
      841 gtatcagaac agtttatgaa cctgcaacag gaaaagtggt aactgcattc cccgatagaa
      901 cctctaatcc caaatataac cctgtaaaat aatgagtatt tgaaagatga aaacattaga
      961 cgaacgaata aaaaatttag gaaaatctct tgaagataga attgacgcta atctaattga
     1021 tgccgcacta gagtacatta ctttttcaga acgtttgcta gcttttgaaa cgctatgtga
     1081 ttatatagaa gatttcaata tccagcttac ggaaaaagaa tcccaagaaa tctcttttat
     1141 taataaggaa tttggaattg aaagtacatc agattaataa atattaggtc ggtttaaaaa
     1201 cccaacaatc aaaaagtcgt ctgaaactgt gtttaattta cagacgacct ttccttcatt
     1261 tgaagcagga tattgagaac taagttcttc aaaaatccta cacctactcc ttccacggca
     1321 gcaccttggt caaaacggca gacggctaca aagccattgc ccatatccga gccggcgaga
     1381 gcgtcctctc caaggacgag gcaagtggaa aaacgggata caaacccgtt accgcccgat
     1441 acggcaatcc ctatcaagaa accgtttaca ttgaaatttc agacggcatc ggcaacagtc
     1501 aaaccctgat ttccaaccgc atccatccgt tttattcgga cggcaaatgg attaaggcgg
     1561 aagacttaaa ggcgggaagc agactgttat ccgaaaacgg cagaacccaa accgtccgca
     1621 acatcgttgt caaaccaaaa ccactcaaag cctacaattt gaccgttgcc gattggcata
     1681 cttactttgt caaaggcgat aaggcggaaa cggaaggggt ttgggttcat aatgattgtc
     1741 cgtatggcgg ttcaaataat ttagagaaag caaaacttcg cgcagaacgg cttagtaaaa
     1801 acgatcgggc aggaaaagac tttactaaag caggaaaaga agccgttatt gatttgaata
     1861 ggattcaaaa taatggtcaa gttaagtgcg ctaattgcgg tattgaaaca attccagcca
     1921 aacaatctat aaaaaatatt tctcctactt caaatgaaag acaagtagat catgtcatcc
     1981 ctaaatctaa gggtggtcaa ggtacaccta aaaacgggca ggtattatgc agaggctgca
     2041 acattaagaa gagtaacaaa tgaaatcgaa acttactgtc gtctattacg atttggaaag
     2101 caatattgca gaagaaatac tgtcaggaaa cataatgcct gatggaaatt ttcttattca
     2161 agagattcct cttttcgcac cgaatttggc tttaaacgac attgttgcca tagaacgtga
     2221 ggataagatg ctgtttttcg accacttgat aaaagcttca ggaaatacca cgataaacat
     2281 cgttgttttg gatcatttcc caaaggattt attggcagcc atagaagaac acagtggtaa
     2341 aatcagaaaa aatggagaga attatttatc ggttaatttt ccgcccaaaa aatataattc
     2401 tgatttaaaa ggaattttaa atagatatga ggaagcaaat atcctcagct acagggaagc
     2461 ttgtttgggc ttctcttaat cgatctataa gtaagaatgg attacgttag attgtaaacc
     2521 taacgattaa aaggtcgtct gaaaccgtgt ttaattttca gacgacctat ttccttcatt
     2581 tgaaacagga tattgggaat taagtttttc aaaaatccta cacctgctcc ttccacggca
     2641 gcaccttggt caaaacggca gacggctaca aagccattgc ccatatccga gccggagacc
     2701 gcgtcctttc caaggacgag gcaagcggag aaacgggata caaacccgtt accgcccgat
     2761 acggtaatcc gtatcgggaa accgtttaca ttaaagtttc agacggcatc ggtaaaatac
     2821 agaccctcgt ttccaataaa atccacccgt tttacagtca aggaaaatgg atacaggcag
     2881 gtcgtctgaa aaaaggcgac accctgcttt ccgaaagcgg cgcaaaacag acggttcaaa
     2941 acattacctt caaacagcag ccgctcaaag cctacaatct gaccgttgcc gactggcata
     3001 cttacttcgt caagggtgat agggcggaaa cggaaggggt ttgggttcat aatgattgtc
     3061 cgtatggaga aaaatatagg acagaagttg gttcttatac taatactcat gaaagtggtc
     3121 gaacttattc tggaaagggc acaagacaac gttcccaaat ctctggagca agagaagcta
     3181 gggttaataa tgaccctcat atagctacag attttactcc tgccaaaaat aatcgagagg
     3241 cattcaaaga tgaatctaga agaattgacg ctcacggtgg ttccaaaagt accaataatt
     3301 acaatcgaat tgaaagcccg gggaaaaaat accgaaaaca agatggagat taaaaatgat
     3361 tgttgacgaa aaatctactt cagatgtact gaaacaatat caagaactag ggtttgattt
     3421 gacaaaacct atgataatag agttttttat aggaggttct caaaagaatc tgcaatcaat
     3481 agaaagccga ctcatttctt atcggcaaga ttttcaaatc agtattgagc aaaatgaatt
     3541 cggagaaatg tggacttgct attgttccgt caatatcgtc ccttcccttg aaaacatttt
     3601 agctattgaa actacgttat ttggtattgc acaaaaaaat gattgtaatt atgaaggttt
     3661 tggttcgtat gggaactaac aaaaagtgta ggttgggttg aaaaaaccat attcccatta
     3721 aagattaggg tgtgtctatg gacataaaat tcaatacttt gggggttatc ctgaatgggg
     3781 tcaatcctga agaaaaattc ataaaaatta ttgatgatca ggaaaatacc gggggatttc
     3841 tgatactgtt atcttcaaat gacaaattct catcatttga cagttacgat gactgggtcg
     3901 aaaacttaga aatattaaaa gaatacctgc aagaatctca ttggataata aagtgggtcg
     3961 gatagtcggc aaacgtagtc cgtctgaaac gcttttactt ttcagacgga ctttctatca
     4021 atgggaacaa ggcgttttgt ctcaatctgg tctatgatta ttcgatacag tgatgggaag
     4081 ctaagaaaaa tatgattaat catattgaaa aatatatagg cactatttct catggctcga
     4141 aaagcgattc gggaagtcag tacaaattaa atatcgcagc tattccctct tcccctaaca
     4201 gggatttaaa aacttatatt actttagagt taagcaaaca tgatctgaat tataaaagca
     4261 gatttgaaat actttttgta tgttctttaa aatatgatga aaatcagatt ttcccatttt
     4321 taagatggct ggcagaaacc atcattgaga ataaaaaaat ccttcttcga ggacaggtta
     4381 tttatttgcc tagaagcatt gttgattcaa caaaaatgga tgcactgtat gtttccgccc
     4441 cattttattt cgacgatgat tttcaggtct gttatggcga acactacaat atcgttttcc
     4501 ctttgcttgt ccccttgtat aaacaggaag ccgaattggt ggaaaaaaag ggttggaatg
     4561 cttttgagca gttcttgctg gataatgaag ttgacaacct ttcggatatg aatcggaaac
     4621 cgtttgtttg gtaagggctt tcttaaattt gattaaaggt cgtctgaaac cgtgtttaac
     4681 tttcagacaa cctttccttc atttgaaaca ggatattggg aactaagttc ttcaaaaatc
     4741 ctgtctccgt cccttatacg tcggtgcttt agtcaaaacg gcagacggct acaaagccat
     4801 cgcccatatt caagccggag accgcgtcct ttccaaggac gaggcaagcg gggaaacagg
     4861 atacaaaccc gttaccgccc gatacggcaa tccgtatcgg gaaaccgttt acattgaagt
     4921 ttcagacggc atcggcaaca gccaaaccct gatttccaac cgcatccacc cgttttattc
     4981 ggacggcaaa tggattaagg cggaagactt aaaggcggga agcagactgt tatccgaaaa
     5041 cggcagaacc caaaccgtcc gcaacaccgt tgtcaaacca aaaccgctca aagcctacaa
     5101 tctgaccgtt gccgattggc atacttactt cgtcaagggc agtcaggcgg aaacggaagg
     5161 ggtttgggtt cataatgatt gtccgtatga taaaggcaac caacgatata aagacgcttc
     5221 ttatcatggc aaaaatgata attctgtgaa aagtagagca ccaacaaacg gacaagcagc
     5281 tcttgataat tccgttcaag ttaaatcaac ttctcctcga agagttgggg ttgataaagc
     5341 caataatgaa atcgttgtat tagacaaaac tcaaactttt aataacggtt ctgcggaata
     5401 tcacgggcat gtcagaagtt ggcaagattt gcataccgat cagaaaaatg ctttaaaaaa
     5461 agcaggattg gattagttaa ttcaaaagga aaaattaaaa aatgactgat aaaagtaaaa
     5521 cagaaaagtt gatttcttct gatgataaac aaagtgttat agatggcatt cttgatatgg
     5581 tatttaattc caaagcatat gaagtaccgt ggatttctga gaaattgatg gaattatcga
     5641 aaaataaaga cttggatatt gccggattat cgctaacctg tttcggacat ctcgccaggc
     5701 tacattcaaa tatcggtgat tacgataaag ttatttcttt actacattca aagcaagatg
     5761 atccagagct tcaaggtagg gctgaagatg cgttagaaga tatttcttta tttttatctg
     5821 aaaatcatta ggaaccgtag gtcgggttga aaacccaaca atcaaaatgc cgtctgaaac
     5881 cgtgtttaat tttcagacgg catttccttc atttgaaata ggatattgag aactgagttc
     5941 ttcaaaaatc ctacacctgc tccttccacg gcagcacctt ggtcaaaacg gcagacggct
     6001 gaaaagcaaa caccgtccgt cgtgttgccg tttgcggatg agtacgggtc aaccccaatg
     6061 ccgccgaaac cgtcgaagcc gccttcaaca ttgccgccgc caaagccgcg aagttggcaa
     6121 aaacggtaaa accggggaga taaaagccga tggcaggaaa gtaaatgtga ggatagacag
     6181 tacggaggca gacctgcttt atccggcagg gcaataagaa aacaaaaatc agatatggaa
     6241 aacgattgtg aagattaaac cattacaatt ttctaacaat aatcacagat tttatgtgga
     6301 caaaatttgt gttaccgaac aagatgtgaa cattttggca tccgaccgaa attatgaatt
     6361 aaatattgaa atatttattg acaatataat tcattttcaa ataacagatg aatcttataa
     6421 agtaaaattt tcagaatatt tatttgaaaa taaagaaaaa aatgattggg atagaaatcc
     6481 tgctataaat tattttttcg agataataga tgacagttat atggactggt tgaaagaaga
     6541 aagttttgat ttttttgaaa agaaatatta taaggcttat attttctttt ttagcgattc
     6601 tgtaatagaa gttatcagct cgacagaacc tgtattttat tcaaaataac aaattatcaa
     6661 acaaagctct gattaaaaac ccaacaatca aaatgccgtc tgaaacaata ttcggctttc
     6721 agacggcatt tttgatacaa agcaggtaac caaaagagcg tttgacggca aaggagaatc
     6781 taaaataccg gatgtatcgg ttgggaagca atggataaag gtaaataatt atgtggaaaa
     6841 ttagtaaaga aaattgtgaa gatttaggat ttgcaatagt ctgtatgttc tatgatgcta
     6901 ttaatctttc tgaatttaaa ttatggttgg atatagttgt cagagatact cctattgata
     6961 caattccatt gtatattttt gatttgattg attttgataa gagtataggg gaaatttatg
     7021 atgtaattgg agtcgttaat tatggttaca tttcaaatga tcaaaaaaat gcattaacgg
     7081 gcattgcctt cttaaggggg atagatgtct atgatccgcc tatttcaaaa gaaaaagcat
     7141 taaaagcctt agagaaatat cctgaaattt atcagaggtt tcagcatttc tttccttttg
     7201 tagagcttcc gcttctttaa aggacaagat gccgtctgaa aagttttcag acggcatttt
     7261 ttatttcttc cagtaggcgg gagtgaagag gatgaagacg gtgaagattt ccagtctgcc
     7321 caagagcatg gcggtaacgc agatccattt ctgcatcacg tccaaaccgg cgtaattgct
     7381 ggcgggcccg acttcgccca ggccggggcc ggcgttggtg atgcaggcga tgacggcggt
     7441 gaaggcggtg gtaaattcca taccgctcgc catcagcagg aagctgaaga ggacgacggt
     7501 cataaagtag atgaagatga aggacataac ggtcagcgcg aggcggtcgg gtatggcctt
     7561 gccgctgatt ttgacggtgc ggacggcttt ggggtgcagc agcaccatca tttcgcgcag
     7621 gctgaatttg aacaggacga gggcgcgtat ggttttgatg ccgccgccgg tcgagccgga
     7681 gttggcgagg atgttggcga ggaaaaacat ccacagggaa atcaggagcg gccattgtgc
     7741 gaagtcggtg ttggccagcc cgtttgccag tccgatggag acgaagttga aggcggtgta
     7801 gcgcagggat tcggtaaaac cggcgtaata gccggtgtgc cacaggtaca gggcggcggc
     7861 aaggatgctg ccggagagca gcagcagcat cgtccggcat tcttcgtctt tccaataggt
     7921 tttgaggctg cggctgttga gggcggcgaa atggttggca aaattgatgc cgccgacaat
     7981 ggtgaaaacg atgatgaccg cttcgatgag gggggagttg taataagcta tgctggcatc
     8041 gtgggtggaa aacccgccca gcgagagggt cgccatcgcg tgacagacgg catcgaacca
     8101 gctcatcccg gcaaaatgca ggcaggctgc cgcgaggatg gtgatcaggg tgtagccgaa
     8161 ccaaagtttt ttcgccactt gggaaatgcg cggcgacatt ttgctttctt tgtcaatgcc
     8221 ggggatttcg gctttgaata attgcgtgcc gcctacgccg agcataggca ggatggcgac
     8281 ggcaaggacg atgatgccca tcccgcccag ccagttgagc atatgccgcc aaaagttgac
     8341 ggagggggcg agcccgtcga cgtgggggat gacggtcgcg ccggtggtgg tcagtcccga
     8401 catcgattca aaaaatgcgt cggtaaagcc catattcggg aaatacaggt acatcggcat
     8461 cgccgccata gcggcaaacg ccagccacaa catcaggacg agggtgaagc cgtcgcgcgg
     8521 gcgcagttcg cgcctgaacc ggagggtggc gagccggacg atgtacgagc cggaaagggt
     8581 aacggtcgcg gtggtggcga aggcggtgta cgcgccgtcc gaaaaggcgt aggagagggc
     8641 ggcgggtatc agcaggataa aggaaaacag catacccagt cgggagagga cgtgggcgat
     8701 gggcaggatt ttgtgcatag tggggcggtc cgttattttg cgaagctttt ccagtctatg
     8761 ccgccggcgg cttggacttg ggtaatttct tccgtttcga ggttgacggc ggtcagctgt
     8821 ccgccccaca gcgcgccggt gtccagcgag atgacgttgt cggcattcgt gtagcccagc
     8881 gaggaccagt gtccgaagat gatggtgcgg tcgaggtttt gccggtcggg ggctttgaac
     8941 cacgggcgca ggtaaggcgg catttttttc actgtggatt tgtagtcgaa atccagttcg
     9001 tttttaaagg tcagggcgcg catccgcgtg aaggcgttga cgatgaagcg caggcgggca
     9061 tagccttcca aaccttcgtc ccacgcggcc ggtttgttgc cgtacatttt ggagaagaat
     9121 ttgacgtatt ttttgccgcg cagttcggct tcggcttctc cggcgagcga ttcggctttg
     9181 gttatgcgcc attgcggcag gatgccggcg tgtaccatca cgcggctgcc ctcgcgtatc
     9241 aacagcggtt gcgcgcgcag ccagtcgagc atttttttcc cgtcggggtg tttgagtatg
     9301 ggttcgattg tgtcgctgcg tttgggcgca ccttcgccgc agccgacagc gagcaggtgc
     9361 aggtcgtggt tgccgaggac gatttgcaca ctgttttcgt gccggatgca gaattgcagc
     9421 gtttcgaggg atttcgggcc gcggttgacg atgtcgcccg tcagccagag ggtgtccgtg
     9481 ccgtggttga aaccgatttt gccgagcagc gcggtcagtt cgtcgaaaca gccttgtatg
     9541 tcgccgattg cgtaatgtgc cattgcagat gttgtgaagt gggaaagtgt tgcggttcgg
     9601 acggcatggt tttgaaatat catgcagtcc gaacgtggaa ttatgcgttc aaaacgagga
     9661 cggcttcggc ttcgacctgc acgcctttgg gcagcgaggc aacgccgacg gcggcgcggg
     9721 cggggaaggg ctcggcgata aattccgcca tgacttcgtt gaagacggcg aaattgccca
     9781 agtcggtcag gtaggcgttg agtttgacga tgtcggccag cgtgccgcct gccgcttcgg
     9841 cgatggcttg caggttttgg aacacttggc gcgcttcggc gcggaaatcg ccgttgccga
     9901 cgacggtcat cgtggcggga tcgaggggga tttgaccgct catgtaaacg gtgtcgcctg
     9961 ctcggacggc ttggctgtac gcgccgatgg cggcgggggc tttgtcggtg tggatgatgg
    10021 ttttggacat ttcggattcc tcaaaaaata gggcggcaga agccgcagca ttcgggatta
    10081 tcgtacaaaa ccgccggctt gtgtagttgc ggtggcagaa aacaaaaccg ccgaaggctc
    10141 ggcggtttgc ggaataaggc gcatatcaga atttgacgcg cacgccggcg gacagttcgc
    10201 cggaacggac gtttttgaca gtgttgactt tgccgatgta gttgtagcgg tagccggtat
    10261 ccaaatcgac attcggggta acggcatagc ttacgcccgc caatacgccg aggccgatgg
    10321 aggtttggct gaagttgtcg ctgccgccca agtcgacgga ggcgcggttg aggctcaagc
    10381 gcgcgccgag atacggtttg acgggcgatt gggtgtcgaa gtcgtaaatg gcggacgcgc
    10441 cgatgctgta aagtttgaaa tcggtggatg gggctttata gtttttgtag cgcgtgtaat
    10501 cgactgcgaa gcggaggtcg ttgatgcggt agcctgcgga gatgcgcggg ctgaagcctt
    10561 tggcagaacc taaagagctt gaggcttttg cgtgtgcggc atcggcttgg acgtaaaagc
    10621 cggatgcgcc ttccgccagt gcggcggccg ggagagcgag ggcaatcagt gtggcaagtg
    10681 cttttttcat attttggttc ctttatggtc agttagaaaa attgttaaga atccgttaaa
    10741 gaattctgct ttattatact taaattttct ttttgcatcg taatattttc aatacttcaa
    10801 gatacgtagc ggtatccggc tgctttgccg acggcaaagc cgttaacccg cgcgttgcct
    10861 ttaaatggtg gcggcggcat cacgcggcgg atgggtgaaa cttgcaaacg gtttggaaaa
    10921 aacagcggta tctgtcggat tgttgcaggt gcaggcatac ggttttttgt gtgcgtctgt
    10981 gccttaagcg tcggacattt ccggcggcgg ctgtgccgtc tgaaacgccc ggcgggggat
    11041 gcggctgcgt tttccatcga taagcatatt ttccggacgc gttcggggcg ggttttcccg
    11101 ggcggccgcc gatttgtttg agcttatata gtggattaac aaaaatcagg acaaggcgac
    11161 gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
    11221 gaatcattct ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat
    11281 aaaaaacatt gcagcaaatc gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg
    11341 gtcgggtcgg tttccagcag gcctttttgc cttgccgttt cgatttgcgc catgattttg
    11401 gcactcggta cgcccgtacg ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc
    11461 agggcgttca tcatgaattc gaacggcaaa tcttcggcgg caacggtttt gcgttcgacg
    11521 gcttcactcg gttggctttg cattaaggcg aggtagtcgt tggggtggcg gcggcggacg
    11581 gtgcgctcga tgcggtcggg ataggagatt ttgccgtgcg cgcccgcgcc gatgcccaaa
    11641 taatcgccga actgccagta gttcaaattg tggcggcatt gtttgttgtt ttgtgcgaag
    11701 gctgatgttt cgtaatggat aaagccggcg ttttccaatg cgccgtgtac cgcgtcttcg
    11761 atgtcgaggg cggcttcgtc ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg
    11821 ttcggttcca tcgtcagatg atacgcgctg atgtgggtcg cacccgtagt gatggcggtt
    11881 tgtacgtcgt ccaatgccgt ctgaacggtt tggttcggta gggcgtacat caagtcgata
    11941 ttgactttat caaataattt taaggcggta tcgatagcgg ttaaggcttc cttgccgttg
    12001 tggacgcgcc ccagccttga gagcatatcg tcgttgaaac tctgtacgcc gatagaaagc
    12061 cgcgtgatgc ctgcgtcttt aaatccttga aacttctcga tttcaaatgt ccccggattg
    12121 gcttccaagg taatttccgc ttcgggctgc aagcgcaaca gcgaacgcac gccgctcaat
    12181 aaacggtcaa tcgattccgc ctgaaacagg ctgggcgtac cgccgccgaa aaatatcgtt
    12241 tccaccggcc tgccccaaat attgggcaat tcaagctgca agtcggtcag cagcgcgtcg
    12301 atataggcgg cttcgggcaa tccgtttttc aggctgtggg aattaaagtc gcaatacggg
    12361 cattttttga tgcaccacgg gatgtggata tagagcgaca ggggcggcag ggcggtgagc
    12421 cgggtgcggt ttggaaagga aatggcgtgc atggtgtggt tcggaaaagt ggacaatgcc
    12481 gtctgaaggc ggttcagacg gcacaggttc agccgagcag ggtaagcagt tcggcttcac
    12541 tgaggacgga aacgcccaag gcattggctt tttccagctt gctgcccgcg gcttctccgg
    12601 cgacgacgta atcggttttt ttggaaacgc tgccggaaac tttgccgcct gcggcttcga
    12661 ttagggcttg ggcttggtcg cgtttgaggg tgggcagtgt gccggttaac acgaaggttt
    12721 tgcccgccac ggctttattg atgccgtctg aaccttgcgc cgcctcatct tcagacggca
    12781 tttgcgcgaa gaaggttttc aggttttcga gcagggcggc gttttgtgtt tcgctgcgcc
    12841 acgcctgcca gtcggtgggg agggctttgt cggtttgcag cccttctatg tttttgccgg
    12901 cgagttccca taaggcttgg gctttgtttt cgctgatttt gaaaccgggc aggcgggcaa
    12961 tccagcgttg cggttcggca tattgtgcgg taggcaggct gacggcttgg gcttgcgggg
    13021 caacgcctgc ggcgagcagc tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt
    13081 gggcaatgga acgcgccact accgtaccga tgtcgggcag gcaggcgagg acgggttcgg
    13141 gggcgcggcg gacgcgttcc aatgtgccga atgcctgtgc cagcgttttg gcggtgcgtt
    13201 cgccgacgtg gcggatgccg agcgcgaaca ggaagcgggc gagttcgggt gttttgccgg
    13261 attctatgcc tgcgaggatg ttttccgccc acttggtcgg ttgtttttta tgtttgcccg
    13321 acgcgccgac cgaactgtct tcagacggcg tttgatccga ttcggcaacg gttttgtccg
    13381 ccgtttcctt cattttttgc agggtcggaa tatcgaggcg gtagaggtcg gcgaagtggc
    13441 ggacgaggtc ttgggcgaca agctgttcga tttgtttttc acccaagccg tcgatgtcca
    13501 tcgctttgcg cgaggcgaag tggattaagc cttgcgcgcg ttgtgcctga caaagcatgc
    13561 cgccgctgca tcgggcgacg gcttcgcctt cttcgcgttc gatttcgctg cggcagatgg
    13621 ggcagtgggt cggcaggcgg tagggcttgt agagcggaac ggattgggtt tggtttgcag
    13681 ccggtgcttc ggcaaacaaa tcgtcctgcc gatgcccgat gccgtctgaa acagcaacgg
    13741 cggtttcctg catcgggcgg cgttcaaaaa tcacacgcac gacttcggga atcacgtctc
    13801 cggcgcggcg cacgacgacg gtatcgccga cgcgcacgtc tttacgcgat acttcgtcct
    13861 gattgtgcag ggtggcgttg gtaacggtta cgccgccgac gaatacgggc tgcaggcggg
    13921 caaccggcgt taccgcgccc gtcctgccga tttgcacgtc aatcgcttcg acaatggtca
    13981 gggcttcttc ggcggggaat ttgtgggcaa tcgcccagcg cggcgcgcgg gagatgaagc
    14041 cgagttcgtg ctgttgcgcc aagctgttga ctttgacgac catgccgtcg atttcatagg
    14101 gcagttcggg gcgtttttgc tgcatgtgtc cgtaaaacgc caatacttca tcgatatttt
    14161 tgaaacagcc gaaattgcca ttgggcagac tgaagccgag ttcttggaaa taggcgagtt
    14221 cctggatgtg ttcttccgcg acgaaaccat cttgctggcg ggcgacggag taggggaaaa
    14281 agtgcagttt gcgttgcgca gtgatgcgcg aatcgagttg gcgcaggctg ccggcagcgg
    14341 cgttgcgcgg attggcaaag ggtttttgcc cgttttcggc ttgtctttta ttgagggcgg
    14401 caaaatcggc tttttgcatc agtacttcgc cgcgtacttc gatgagttcg ggcgtatttt
    14461 cgccgtgcag ccgcaggggg atgttggcga cggttttgat gttttgggta acgtcttcgc
    14521 ccgttgtgcc gtcgccgcgc gttgccgcct gtaccaatac gccgtcgcgg tagagcaggc
    14581 tgatggcgag gccgtcgaat ttgggttcga taacgtattc gggattgccg ccgtccaaac
    14641 cgtcgcgcac gcgttggtcg aaagcgtaca tttcggcgtg gtcgaacacg ccgttttcat
    14701 cttgcgggga aaaggcgttg gtcagcgaca gcatcggcac ttcgtggcgt acttcggcaa
    14761 atcccgccag aggctcgccg ccgacgcgct gggtcgggct gtcgggcagt ttgaattcgg
    14821 gatggtttaa ttccaacgct tcgagctcgc ggaataattt gtcgtattcg gcatcgggta
    14881 cgctgggcgc gtcgagggtg tagtattcgt aggcatagcg gttgagcagg tcggtgaggt
    14941 ggcggatgtg ttgtgcggca aattgtttta tatcactatc agacggttta agaagattgg
    15001 taaagttagt gttatgtttt gagtttggat tcatgagaga aggttttcag acgacctttg
    15061 tctgatacgg gatgaaacgg gcaaaggtcg tctgaaaaat gataggttga aaacagctga
    15121 attttacccg aaaaaaagcg gatatgccgt aacgacatat ccgctttgat tgcattcgat
    15181 tttaggagaa caggcgcaat gcggttttgc cgcccggttc gataccgact ttgagcatct
    15241 cggactgacg agccaataca taagtgcgta cgtctttgag ccattgggtc gaaacttctt
    15301 ccattttgtc gttgaccaga ttcaggttca actggccgga caggcgtacc gccaaatcca
    15361 taaacaaatc gtcgaaggtt ttttcgcctg ccggagagtg cgggatgtcg agcagcatac
    15421 tgaagccttt gtaggactgg ttgtccaaaa gggcattggt aaacggctcg ttgttgagcg
    15481 agcagatgga gaacatggtt gagcccgacg tgtcggtata gtggaacgcg ccgtcgtctt
    15541 ccaaaacgaa acccacgccc gttacggcgg aacgcagttc tacgccgctg atgctggtcg
    15601 gggaaaccaa atggatggcg atggtctggt cgacgcgcgc gcagaatgcg tccagtgcgg
    15661 aagccacttc gataaaggcg gcaaggtcgg tgtgcagcgt ctgaccgccc atgctttgtg
    15721 cgaatgcgtc cacctggcgg ttgaatgcgg agagttcttc ctgcgaggca agtccgttgc
    15781 ggctgactgc ctgaataccc acgataaatg cctgatagcg gatgcccggg atgggttcgg
    15841 caatctggaa atggtcgtcc atggtgcagc cgacaatctg gtagcggcag cggttggaaa
    15901 ggcgcggcag tgcgtgcagt tctttggctt cggtcagcgc gatataggag atgaagtcga
    15961 agcgcacgtc aaaccagggt aattcgactt ttgacagttc tttgagcgta atcagcggtt
    16021 ttgcaggtgt ttgcggaacg ggtgcaggtt ttgccggcgc gtcggcaggt ttcggtgcgg
    16081 aatgtccggt ttggggttcg gaaacggtgt gggcggagtt gccgataatg ccgctttctt
    16141 ccaaggcggt ttcgatttcg gttttgaacg gggaggcttt tgcctgtttc tgcttggcga
    16201 tgtagacggc atcctgttct tgcaggttgc gcatggcggg gtcttggggt tttgccgttt
    16261 ttttgaccgc cggttggggt ttcggcatca tgactggccc gccggacggt tggccgtcgc
    16321 ggacatggct ggttttgctg ttgagcaggg catctttgtc ggagtgtccg aactggtcgc
    16381 gcactttttt gcggtattgg ttttcctgat acatattgta ggcgacaacg gcgaggacgg
    16441 cggcgaggaa cagtacgatg taaatcatgg caatcacttt aaatttcggg atgcaggatg
    16501 cgcgaagtgc gggtactgcg gttaaatcgg gcttgcgttt ccggcagtct gatggaacgg
    16561 ccggttataa cgtttgaatt ataacgaaaa ttgcagggtc tgacagcagt gtgtcgaaat
    16621 aagcggaaat tttccgaaat gccgtctaaa atctgtggtt ttcagacggc atttctgtct
    16681 acgggaaacc ctttctcccg tatccgccgc cagtcgaaaa agtggccggg gtcggttttg
    16741 cggccgggcg cgatgtcctg atgtccggtt actgcggtaa cggggtagcg gcggcagatt
    16801 gcgtccaaca aggcttcgag cgaacggtat tgcgcttcgg caaacggttc gaaatcgcag
    16861 ccttccaatt cgatgccgat ggaaaatgca ttgcattttt ccctgccgcc gaatgaagac
    16921 acgccggcgt ggtatgccat attgtcgcag gaaacgaact gtaccgtttc tccgccgcgt
    16981 ttgattaaaa aatggctgga tacgcgcaaa gtgtgtatca ggctgaagaa cggatgtccg
    17041 tcggggtcga gccggttggc aaacagcttt tccaccgcat ccgtgccgta ttcgaacggc
    17101 ggcagcgaaa tgttgtgcaa cacgatcagg gaaaccgttt cccccgtttc cctcgggctg
    17161 aaattgggcg acggggtatg gcgtatgctt tgaagccagc cgttttgcca gtgtgcttcg
    17221 gcgtgattgt ccatgatgtt cttcctgtcc ggcgggtaat ttgggttata ctgtcgcccg
    17281 aattttaaga cgtattccga atgctgggaa tcctaccatg ttgagaaaat tgttgaaatg
    17341 gtctgccgtt tttttgaccg tgtcggcagc cgttttcgcc gcgctgcttt ttgttcctaa
    17401 ggataatggc agggcatacc gaatcaagat tgccaaaaat cagggtattt cgtcggtcgg
    17461 caggaaactt gccgaagacc gcatcgtgtt cagcaggcat gttttgacgg cggcggccta
    17521 cgttttgggt gtgcacaaca ggctgcatac ggggacgtac agactgcctt cggaagtgtc
    17581 tgcttgggat atcttgcaga aaatgcgcgg cggcaggccg gattccgtta ccgtgcagat
    17641 tatcgaaggt tcgcgttttt cgcatatgag gaaagtcatc gacgcaacgc ccgacatcga
    17701 acacgacacc aaaggctgga gcaatgaaaa actgatggcg gaagttgccc ctgatgcctt
    17761 cagcggcaat cctgaagggc agtttttccc cgacagctac gaaatcgatg cgggcggcag
    17821 cgatttacgg atttaccaaa ccgcctacaa ggcgatgcaa cgccgcctga atgaggcatg
    17881 ggaaagcagg caggacgggc tgccttataa aaatccttat gaaatgctga ttatggcgag
    17941 cctggtcgaa aaggaaacag ggcatgaagc cgaccgcgac catgtcgctt ccgtcttcgt
    18001 caaccggctg aaaatcggta tgcgcctgca aaccgacccg tccgtgattt acggcatggg
    18061 tgcggcatac aagggcaaaa tccgtaaagc cgacctgcgc cgcgacacgc cgtacaacac
    18121 ctacacgcgc ggcggtctgc cgccaacccc gattgcgctg cccggcaagg cggcactcga
    18181 tgccgccgcc catccgtccg gcgaaaaata cctgtatttc gtgtccaaaa tggacggcac
    18241 gggcttgagc cagttcagcc atgatttgac cgaacacaat gccgccgtcc gcaaatatat
    18301 tttgaaaaaa taaaccatgc cgtctgaaaa gtttgtgttt tcggacggca tacccttacc
    18361 ggaactgcaa gcatgaaacc gcaattcatc actttggacg gcatagacgg tgccggcaaa
    18421 tccaccaacc ttgccgtcat caaggcatgg tttgaacgga gggggctgcc cgtgctgttc
    18481 acgcgcgagc cgggcggaac gccggtcggt gaggccttgc gcgaaatcct gctcaaccct
    18541 gaaaccaaag ccggtttgcg tgcggaaaca ctgatgatgt tcgccgccag aatgcagcat
    18601 attgaagacg tgatattgcc tgcactttca gacggcatcc atgtcgtgtc cgaccgtttt
    18661 accgatgcaa ccttcgccta tcagggcggc gggcggggaa tgccgtctga agacattgaa
    18721 attttggaac attgggtgca gggcggtttg cgccccgatt tgaccctgct gttggatgtg
    18781 ccgctggaag tgtcgatggc gcgtatcggg cagacgcgcg agaaagaccg gttcgagcag
    18841 gaacaggcag atttcttcat gcgtgtgcgc agcgtttatc tgaaccgtgc cgccgcctgt
    18901 cccgaacggt acgccattat cgacagtaac cgcagcttgg atgaagtcag aaacagcata
    18961 gaaaaagtgt tggacggaca tttcggctgt tgatgcggca aatattgaaa caagcgcatc
    19021 cgcccgcgcc gaaaatcaaa cggcagtgcc acaggtgaaa atggcggtat gcgccaaact
    19081 ttcggcatga tagaattacg cttagttaca aggcaggatg cgtcggcaat attaacgaac
    19141 cgcccgtaac ccaatgtctc gaaagtgttt cggacagtcc gattcaaatc tttttctcgc
    19201 aacaggattg acacatggaa aactcattga aagaagccgc cctcaagttc cacgaattac
    19261 ccgttccggg caaaatttcc gttaccccga ccaaatctct ggcgaccgac aaagatttgg
    19321 cgttggcgta ctctccgggc gtagccgctc cttgtatgga aatccatgcc gatccgcaaa
    19381 atgcctacaa atacaccgcc aaaggcaact tggtcgctgt catttccaac ggtacggccg
    19441 ttttgggctt gggcgacatc ggcgcgctgg cgggcaaacc cgtgatggaa ggcaaaggcg
    19501 tattgttcaa aaaattcgcc ggtgtggacg tgttcgacat cgaaatcgat gaaaaagacc
    19561 cgcaaaaact cgtggacatc atcgccgctt tagagccgac cttcggcggc atcaacctcg
    19621 aagacatcaa agcacccgag tgtttctaca tcgaacgcga attacgcaaa cgctgcaaaa
    19681 tccccgtatt ccacgacgac cagcacggca cggccatcat taccgccgcc gccgtattga
    19741 acgccctgcg ttttaccggc cgtaaaatcg aagaagcgac tttggtgtgt tctggcgcag
    19801 gtgcggccgc gattgcctgc ttgaaccaat tgctggattt gggcttgaaa cgcgaaaacg
    19861 tgaccgtttg cgactccaaa ggcgtgattt accaaacccg cgaagacaaa gaccgcatgg
    19921 atgagtctaa acagttctac gccattgaag acaacggcca gcgcgtgctt gccgatgccg
    19981 tcaaaggcaa agacatcttc ttgggcctct ccggcgcgaa cctgctgacg cctgaaatgc
    20041 tgaacaccat gaacgaaaaa cccatcgtgt tcgccatggc caacccgaat ccggaaattc
    20101 tgccgccgct ggcaaaagaa acccgtccgg acgtggttat cggtaccggc cgttccgact
    20161 tcccgaacca agtcaacaat gtattgtgct tcccgttcat cttccgtggc gcgttggatg
    20221 tcggcgcgac caccatcaac gaagaaatga aacgcgcctg cgtgtatgct ttggcggatt
    20281 tggcgatgga agaagtaacc gaagaagtgg ttgccgctta cggtaagaaa tttgaattcg
    20341 gcgcggaata cctgattccg actccgttcg attcccgcct gctgccgcgc gttgctacgg
    20401 ctgccgccaa agcagcgatg gaaagcggcg tggcaacccg tccgattgcc gactttgaag
    20461 cctacgccgc caagctgggc gaatggaagc tgtaagccgt ttgcggttta aaatgccgtc
    20521 tgaactgttt tcaggcggta ttttgctgtc agattgatat agtggattaa caaaaatcag
    20581 gacaaggcga cgaagctgca gacagtacaa atagtacgga accgatttac ttggtgcttg
    20641 agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgctgtac tggtttttgt
    20701 taatccacta taaatgaaag atactggaaa atgaaagaga tgaaacctgt ccgttatcat
    20761 attggcgata tgcccgagac ttcaaaacaa accgcctccc gtcatgacga cagggcagtg
    20821 ggtgttgacg atgattgttt tcatgattcc tttggtcaat ttttgtttgg gtgttcggca
    20881 gaggcaaccc gaaccgcgcc aatttctgta aagcgcagtt gcttatttac ctgattggtt
    20941 cgcttatcgg tttggtcttc gcgttgttta taggtgggtc tgtatcgggt acgcatgatt
    21001 aatgccccgg gctgattttg cttcgaggat ttgtatcgaa tatgccgaat tgtttcaaat
    21061 ttcataccgt tatcgaacgg cattggcaaa aaccttatcc ggttttgtct tttctgctta
    21121 agccgctctc cgggctgttt gccaaaattg cggcaaaacg gcggacggat tttttatcgg
    21181 gaaaacggca aagcgaaaag ctgcccgtgc ctgtggtcgt ggtcggcaat attcacgcgg
    21241 gtgggacggg gaaaacgccg attgttgccg cgctggtgtc gggtttgcag gaaaagggcg
    21301 tcaaggtcgg catcatcagc cggggctacg ggcgcaagag caaggcggtt catgtattga
    21361 atgctgagag ccgagcggaa gatgcgggcg atgagccttt gctgctgttc cgcaaaaccg
    21421 gtgcgccgac ggcggtgggc agcagccgtg cagaggcagg cagggcgttg ctggcggcgc
    21481 atcccgacat cggactgatt gtggcggacg acggtttgca gcattacgcc ctgcggcggg
    21541 atgtggaaat tgcggtgttt ccggcggcgg atacggggcg cacggatttg gatttactgc
    21601 ccaacggcag tttgcgcgaa cctttgttgc ggctggattc ggtggatgcg gtcgtcgtca
    21661 gcggcggcaa ggcggatgcg ctgtttaggc cgtctgaaaa tatgtttcac agccgtatcg
    21721 aagcgggacg gatttaccgt ttgaacaatc cgtccgaaat actggacaca ggccgtctga
    21781 aaaatcaaac cgtcgtcgcc gtggcaggta ttgccaagcc ggcgcgtttt ttcgattcgt
    21841 tgcggaatat gggcattacc gtgaagcgaa ccgtcgcgct gcccgaccac gccgacattt
    21901 cggcggcaga tttgcccgat gcggacgcgg tcattattac ggagaaagat gcggtcaaat
    21961 tttcagacgg catttgcacc gataatgttt gggtgttgcc cgtttgtgcg ataatcgaac
    22021 ctgatttggc ggcgtttgtg ttggagcggt tggaaggtgc cgaaggccgt ctgaaagcgc
    22081 ggtttggatg aagtgataac ggactggaat aagaacgccc tacgccgtca ttcccgcgca
    22141 ggtgggaatc tggatcttga tttttcagaa atgaccaaga ggtttccgaa gtcctaaatc
    22201 tccggattcc cgcctgcgca ggaataatgg aagccggttg tattttttat ctgcattaat
    22261 catttgttaa aggattgaat attaaattga aaaccttgtt attgcccttc gccacgctgg
    22321 cattgtgcac caacgctttt gccgccccgc ccagcgacgc gtcgttggcg cgttggctgg
    22381 atacgcagaa ttttgaccgg gatatagaaa aaaatatgat tgagggcttt aatgccggat
    22441 ttaaaccgta tgcggacaaa gcccttgccg aaatgccgga agcgaaaaaa gatcaggcgg
    22501 caaaagcctt taaccgctat cgtgagaatg ttttgaaaga tttgattacg cccgaagtga
    22561 aacaggctgt ccgtaatact ttattgaaga atgcccgtga gatatacacg caagaagaaa
    22621 ttgacggcat gattgccttt tacggttcgc ctgtcggtca gtccgtcgtt gccaaaaatc
    22681 cgcgcttaat caagaaatcg atgagtgaaa tagcggtatc ttggactgca ttgtcaggga
    22741 aaatcgcgcg acatcatctg cccgcgttta cggaagagtt gcggcgcatc atctgcggcg
    22801 gtaaaaatcc cgatgcgggc tgtaaacaag ccggacaggt tgggaaaagg catcagaaat
    22861 aaatgatagc cgtctgaaat attgaagagg gcatccgatt gattgaacca tcaaacccga
    22921 aagcaaccct atggaaaaaa aattcttaga catcctcgtc tgccccgtta ccaaaggcag
    22981 gctggaatat catcaggaca aacaggaatt gtggagccgt caggcgaagc ttgcctatcc
    23041 gattaaagac ggcattccct atatgctgga aaacgaagcg cgaacgttga gcgaagagga
    23101 actcaaagca tgaccgaatt cgtcgtattg attccggcgc ggctggattc gtcgcgcctg
    23161 cccagaaaag ccttggcgga catccacggc aaaccgatgg tcgtgcgcgt tgccgaacag
    23221 gcggcaaaaa gtaaagctgc gcgcgtcgtt gttgccaccg accatcccga tattcagacg
    23281 gtctgtcagg cgcacggcgt cgaagtcgtc atgacttcaa accggcacga aagcggcacg
    23341 acgcgccttg ctgaagctgc cgccgcgctg aagctgccgc aacatctggt cgttgtcaac
    23401 gtacagggcg acgagccgct gattgccccc gaactcatcg accgcaccgc cgaagtactc
    23461 gtcgaaaaca acgtccaaat ggcgaccgcc gcccacgaat tgcacgattt cgacgaattg
    23521 atgaatccca atgccgtcaa agtcgtcctc gacaaaaacc gcaacgccat ctacttcagc
    23581 cgcgccccga ttccctatct gcgcgatgca atgcgtgccg gaaaacgcga aatgccgtct
    23641 gaaaccgccg tcctgcgcca tatcggcatc tacgcttacc gcgccggctt cctgcaacgc
    23701 tatgccgaaa tgagcgtttc gccgctggaa accatcgaat cgctggaaca gctgcgcgtc
    23761 ctgtggcacg gttatcccat tgccgtcgaa accgccaaag aagcccccgc cgccggtgtg
    23821 gatacgcagg aagatttgga tagggttcgc gctgtatttc agaccgtata aaacaggttc
    23881 aaagggaaaa gatatgcagc aacatattga aaagtggcaa cacttgagcc gggaagaaca
    23941 gaaaatcctt gccgaagtat ggggtctcgt gcaaaacgat gatcaggagg ttcactatga
    24001 aatgcttaaa ttgaacgcac ccgacgaagc cagtggcgaa ttttggttca gaatggcaga
    24061 aacactcagc accctgccgc ccaaccgttc cctcgacctt agaatgaacg gcggcaggct
    24121 ggcgaccgcc gtatccatcc tttccgtcat gattgaagac aatcccgaca taccgcagct
    24181 ttgggcgcaa aaaattaccg ccctcaatta tagtggatta aatttaaacc agtacggcgt
    24241 tgcctcgcct tgtcgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt
    24301 gttaatccac tatatttggc acacggacac aaagcccgtg ccgacggttt ggcacaacag
    24361 cccgacaaag cggcagaagc caacgaggag gaatacctga ccaaagccct gtcgcaaaac
    24421 ctgctgtcaa cattggatgt cgcgcttgca cgttttcctg aagacgcgtg gtttcaggaa
    24481 atcaaacagg atgcacaaaa gcattttgct tgaggatgtg gcagtcagga atatttccat
    24541 tcaggaagaa aagaagtgcc tgattgggta taatcagggt aaatcttatt ttatttcaaa
    24601 agattaatat ttgctttcca tttttccttg acggtatcgg aaaagttgat tatagttaca
    24661 gcttccttag gagtaatggc tgagaggctg aaggcacttc cctgctaagg aagcatgtgg
    24721 ggtcaacctg catcgagggt tcgaatccct cttactccgc cagataaaaa atagacgctg
    24781 tgttttacag tgtctatttt tttatgcaat tttatagcgg gttggtgcaa aaccagtatg
    24841 gtattgccct gtcttgattc tgaattttgt tatagtggat taacaaaaac cagtacggcg
    24901 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
    24961 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
    25021 ctataatccg agatgcttgc cgtttatttc cgcctcgttc aaacggcggc tctgatttgc
    25081 gcggtttctg tttgccgtat tcgcctatcc gtaccgcaaa tgttatactg ggaaaaattt
    25141 actgattgtg ttttacggca tatttgccga taggatggaa gagacaaatg agcagaatcc
    25201 ggcaggcttt tgccgctttg gatggcggaa aggcattgat tccctatatt acggtgggcg
    25261 accccgatat tcggacaact ttggcattga tgcacggcat ggttgcaaac ggtgcggata
    25321 ttttggagtt gggtgtgccg ttttccgatc cgatggcgga tgggccggtt attcagcgtg
    25381 cggcggagcg ggcgttggca aacgggattt cgctgcgcga tgtcttggat gtcgtcagaa
    25441 aattccgtga aaccgacacg caaacgccgg ttgttttgat gggatatttg aaccctgtac
    25501 ataagatggg ttatcgggag tttgctcagg aagccgcaaa ggcgggtgtg gacggcgtgt
    25561 tgacggtgga ttcccctgtc gaaaccatcg atccgctcta tcgcgagctg aaggataacg
    25621 gggtcgactg tattttcctg attgcgccga cgacgacgga agaccgtatt aaaaccattg
    25681 ccgagctggc aggcggattt gtctattatg tttcgctcaa gggcgtaacg ggcgcggcaa
    25741 gtttggatac ggatgaggtt tcgcgtaaaa tagagtattt gcatcagtat atcgatattc
    25801 ccatcggtgt cggtttcggc atcagcaatg cggaaagcgc gcgcaaaatc ggccgggttg
    25861 ccgacgcggt cattgtcggc agccggattg tgaaagagat tgaaaacaat gcgggcaacg
    25921 aggctgcggt cgtcggtgct ttggtcaaag agttgaagga tgccgtgcgc tgattctgtc
    25981 acgcattcta aatattttag gagttgtcca tgagctggtt agataaaatc ctgccaccca
    26041 aaatcaaaaa ccgcagcaaa gacggttctt ccaatgttcc cgagggtttg tggcacaaat
    26101 gtccgtcttg ttcggcaacc gtttattcga ccgagttgca gcagaacaat caggtttgtc
    26161 cgaaatgcaa ccaccacaat ccgttgtcgg cacgacaacg cctgaacctg cttttggatg
    26221 aggatggcag ggaggaagtt gccggaaatg tcaaaccgat agatcctttg aagtttaaag
    26281 acagtaaaaa atatcccgac cgtttggggg cggcacgcaa gctgaccggg gaagatgacg
    26341 cgctggtggt gatgaagggg atgatgaacg gcctgcccgt cgtcgttgct gcgtttgaat
    26401 tccgctttat cggcggttcg atgggttcgg ttgtgggcga acgattcgta caaggtatcc
    26461 gtcgggcggt tgccgacaat tgtccgttcg tctgtgtggc ggcttccggc ggtgcgcgta
    26521 tgcaggaggg tgtgaactca ttgatgcaga tgacgaaaac cagtgccgcg ctgcatttgc
    26581 tgacggaaaa acgcctgcca tttatatcgg tgttgaccga tccgactatg ggcggcgtat
    26641 ccgccagctt cgcatttttg ggcgatgtcg tgcttgccga accgaacgcg ctgatcggtt
    26701 ttgccggtcc gcgcgtgatt gagcagacgg tgcgcgaaac gctgccggaa ggcttccaac
    26761 gcgccgagtt cctgctggaa aaaggcgcaa tcgaccagat tgtcgaccgc cgcgatatga
    26821 agcggcgcat cagtgatttg attacgctgt tgtgccgtca ggacaaagtt tccgccgctt
    26881 gatggctgat gaatcgaata ccgtctgaaa ccgatgtttc agacggtatt tttgtgtctg
    26941 gttatttgtt gtgcggcttt atcgacgggg catagcgtcc ggcacgttct ttcaggcgtt
    27001 gtaccaaacc tttcgtgtcg gcgggtacac cgccctcgca gaatgcctga tacaggacgg
    27061 tgcgcagtgc gtcgttgcgg cttaatgtac cgcctatcgg tttccattcg gcgtttcggg
    27121 gctgtatcca gcggcggttg accgtgtcgc cgtagccgaa cactttatag gaggaaggtt
    27181 tgccgttgcc gaacctgatg gagaagcggg cgcagaatat gccttcggca gtcaggttgt
    27241 cgtagccttt gtcggagcgg atattgagaa tgtagcggat gctgccgtcg ggcgcgggca
    27301 taatttgcag gctgtcgagc aggattttcg gctgtttgcc gtaattttca tccacataaa
    27361 tgtcgaacca gccgtccgag tgcgtatcgg gcagcggcgg cagtttggcg gtatgttctt
    27421 taaattcgcg ggcggcggct tcttcgggcg tttcgcggta gcgggtgttg atcggcgtgt
    27481 ctttttggct gaagccggca gcaagggacg tgccagcggc gagtgcgagc agcaggaggg
    27541 tggtgcggcg cataagtttc tccaaattga aaacggcgtt attttatggg ttggcaaagg
    27601 gggctgcaag caactggggt ataatctccc cccgattccc attttttaaa cggtacaaac
    27661 gatgaacagc gaaactttag acgtaaccgg gctgaaatgc cctttgccta ttttgcgtgc
    27721 caaaaaggct ttggcgcaaa tgcagcaggg cgacgtgttg accgttctgg caaccgacgg
    27781 cggcgcaccg ggggattttg aggctttttg ccgccaaacc ggtcatgtgc tgttggatgc
    27841 ttccgaacag gacggcgtgt tcacgctggt cgtcaagcac aaataaatgc cgtctgaaat
    27901 gcggatgtcc cgcccgacgg cgttgttttt gaatatcgta tgtgccgcgt gccgtttcaa
    27961 aaacaaaccg tccggcgtgc agtgtcggca tttcgggcgt atcgccgccg atttgttccg
    28021 gaatggctgt taaccgcctt gccgtcggca aagaagcaaa acccaagcac aatcaaaatc
    28081 taaaggctgt gtttgaagat tccgttgatg caacccaatc agtcggacaa aatgcctttc
    28141 atccaatgga accggtttcc gaccggacgg aataaccggc tttcccccgg caaacggatg
    28201 gaatcgaccg ggtattcaaa cgcagccaaa acctaaaaag gaacaaccat gcaaaccctg
    28261 accattatcc gccccgacga tatgcacctg cacctgcgcg acggcgacgc gctcaaagcc
    28321 gttgcccctt ataccgcccg ccaaatgggg cgcgccgtca ttatgcccaa cctcaaaccg
    28381 cctgtcgtca gtgtagccga cgcgcttgcc tacaaagcgc gcattatggc ggcgttgccc
    28441 gaaggcagcg cgtttgagcc gttgatgacg ctttatttga ctgataacgc cacgcccgaa
    28501 cttgtacgcg aagccaaagc cgccggcatc gtcgccttca aactctaccc tgccggcgcg
    28561 accaccaatt ccgattccgg cgtaaccgac ctgttcaagc tcatccccgt gttggaagaa
    28621 atggcgaaac agggcatttt gttcctcgtt cacggcgaag taaccgaccc cgaaatcgac
    28681 atcttcgacc gcgaagccgc ctttatcggg cgcgtgatga aacccgtttt ggcgcaagtg
    28741 ccgaatctta aagtcgtgtt cgaacacatc accaccgccg aagccgcccg cctggttttg
    28801 gaagcaggcg acaacgtagc cgccaccgtt accccgcaac acctcctgct caaccgcaac
    28861 gacctcttgg tcggcggcgt gcgcccccat catttctgcc tgcccgtact caaacgcgaa
    28921 acccaccgtc aggcattggt tgccgccgtt accggcgaga aggcgcacaa attcttcctc
    28981 ggcaccgact ccgcgccgca cgccaagtcc gccaaagaaa acgcctgcgg ctgcgccggt
    29041 atgttcagcg cgatgaccgc cgtcgagctt tacgccgaag tgtttgaaaa agcaggcgcg
    29101 ttggacaaac tcgaagcctt tgcctcgaaa aatggcgcaa ggttttacgg cattccggaa
    29161 aacgccggca cgatcaccct cgtcaaacaa agccaaaccg tccccgcaag tgttccctat
    29221 ggcgacggcg aacttgtccc gatgcgcgcg ggcggcgaaa tcggctggac ggtacagtat
    29281 tgattgaacg gtcaagcaaa atgccgtctg aaaggttttt cagacggcat ttatgtattt
    29341 tccaattcgg attttccgga gtgtcaacgg cgtttaggct cgtcggggcg gatttgggca
    29401 gcgagtttgt cgaggatgcc gttgacgaat ttgtgcccgt ccgtgccgcc gaaggttttg
    29461 gtaacttcga tggcttcgtt gataatgacg gggtagggcg tttcgggcat ggcggacagc
    29521 tcgtggcagg cggtcagcag gacggcgcgt tcgatgggat tgaggtcttt ttcgtcgcgg
    29581 tcgagcaggg ggcggatttt ttggatgtag tccgctgcgt tggtttgcgt accgaagaaa
    29641 agtttgttga acaattcttc gtccgctttg gcaaagtcgg gcatttcgcg gatgttttta
    29701 gcaatttcgg gcgcggcggt gcggttgata agggattggt aaacggcttg tacggcaagc
    29761 tcgcgggaac ggcggcgggc tgttttcata atttttcctt gaaacggttg ggcggcacgg
    29821 tatgccgtct gaaacggaaa gggcgcatcg gtgtacgccc tgtttgttat tcttcgtctt
    29881 caaactgttc ttcgagcaga aggttgacga ggttggcgca ttctacggcg actttggcgg
    29941 catccgaggc tttttcttca atccgttcga ttgcctgcgc gtcgttttcg gtggttagga
    30001 cggcattggc aatcgggata ttgtagtcga gtgcgacgcg gctgacgcct gctccggatt
    30061 cgttggaaac cagctcgaaa tggtaggttt cgccacggat gacgacgccg atggcaatca
    30121 gtgcgtcaaa cttttcggaa gaggcaaagt tcatcagcgc gatgggaatt tcaagcgcgc
    30181 cgggtacggt ggcgacggta atgttttcgt ctgccacgcc caattcttgg agggtgcggc
    30241 agcagacttt gagcatttcg ctgccgattt cgttggtgaa gcgtgcctgt acgatgccga
    30301 tgcggaggtg tttgccgtcg aggttggggg cgatggtgtt cattgggtgt cctttggtat
    30361 tcggaggttt cggaatgccg tctgaaggtt tcagtcttgc ggctgccagt cggcgacggt
    30421 ttggaatgtg ccgtcttcgg caagctccca tgcgctgcct tcgggttggg aaagcagtgc
    30481 ggcggtttca gggttggttt tggcgatgtc ggcgaggctg acgatgctga agttgtccgg
    30541 atcgtcggtg tattcgtcgg tctcgtcgcc gctgaagaaa cgccagccgc tgtcgttttc
    30601 aaaaacgggg gcttcgcggt agaggaagcc gacgggccgg ttttgtttgg cgacggtgtt
    30661 ggtggcgata cagcggtcga gtgccgagga aagtgcttgt gcaaatgcgt tcattacggg
    30721 aatacgttgg gggaaaactt acggatttta ccacgattcg tgcgttgtcg gcagacggcg
    30781 gcggtttggt ggtacaatgt gcgccgtttg cagccttaag gtgtttctgt atttttggag
    30841 tatggaaacg cattcgggct gttttttgcg gaagacggta atgaaagacg atgttttgaa
    30901 acagcaggca cacgcggcga tacagaagaa actgggctac gcgttccgcg atatttcgct
    30961 tttgcggcag gctttgacgc acaggagcca tcatgcgaag cacaacgagc ggttcgagtt
    31021 tgtcggtgat tcgattttga attatacggt ggcgcggatg ctgtttgacg cgtttccgaa
    31081 gttgaccgag ggcgagttgt cgcggttgcg ggcaagtctg gtcaatgagg gcgtgctggc
    31141 ggaaatggcg gcggaaatga atgtcggcga cggcctgtac ttgggggcgg gcgagttgaa
    31201 gagcggcggc ttcagacggc cttcgatact ggcagacgcg atggaggcga tgtttgctgc
    31261 cgtcagcttc gatgccgatt tcaacacggc ggaaaaggtg gtgcgccatt tgtttgccga
    31321 tcgcgtccgg cgcgccgatt ttcaaaatca ggcaaaagac ggcaaaactg ctttgcagga
    31381 ggcgttgcag gcgcgccgtt tcgccttgcc gaaataccgt atcgaagagc aaatcggtta
    31441 tgccaacgac agtatgtttg tcatttcctg cgatttgggc gaactgggtt tcgtgtgccg
    31501 tgccaaaggg acgagccgca aggcggcgga gcaggaagcg gcgaaagagg ctttgaaatg
    31561 gctggaaaag aagctgccgc tgaagaagaa aaagaaatga ggcggcgcgt gaatatgccg
    31621 tctgaaatat ggatatgaaa gcgaatatgg atattgaaac cttccttgca ggggaacgtg
    31681 ccgccgacgg ataccgttgc ggcttcgtgg cgattgtcgg tcgtccgaac gtgggcaaat
    31741 caacgctgat gaaccatctc atcggtcaga aaatcagcat caccagcaaa aaggcgcaga
    31801 cgacgcgcaa ccgcgtaacg gggatttata ccgacgatac cgcgcagttt gtgtttgtcg
    31861 atacgcccgg ttttcaaacc gaccaccgca acgcgctcaa cgaccgtttg aatcaaaacg
    31921 ttaccgaggc actcggcggt gtggatgccg tggttttcgt cgtggaggcg atgcgcttta
    31981 ccgatgccga ccgagtcgtg ttgaaacaac tgcccaagca cacgccggtc gttttagtgg
    32041 tcaacaaaat tgacaaggac aaggcgaaag accgttacgc gttggaggcg tttgttgccc
    32101 aggtgcgcgc cgaatttgaa tttgcggcag cggaggcagt cagcgcgaaa cacggattgc
    32161 ggattgccaa cctgttggag ctgattaaac cgtatctgcc cgaaagcgtg ccgatgtatc
    32221 ccgaagacat ggttacggac aaatcggcgc gttttttagc gatggaaatc gtgcgtgaaa
    32281 aattgttccg ctatttgggc gaggaattgc cttatgcgat gaacgtcgaa gtggagcagt
    32341 ttgaagagga agacggttta aaccgcatct atatcgccgt tttggtcgat aaggaaagcc
    32401 aaaaggcgat tttaatcggc aaaggcgggg agcgtttgaa gaaaatttcc accgaagcgc
    32461 ggttggatat ggaaaaactg tttgatacca aagtgttttt gaaagtgtgg gtcaaagtca
    32521 aatccggttg ggcggacgac atccgcttcc tgcgcgagct gggtttgtag tttttcttgc
    32581 tgaactttac gcaaatgccg tccgaacagg tttcagacgg cattttgttt caatcgggaa
    32641 tatctttgtt aaaaacgggt tgatattatc tgtgca
//
LOCUS       DAXIEB010000008        32308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000008 DAXIEB010000000
VERSION     DAXIEB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.008"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(70..816)
                     /locus_tag="ACFOA3_000328"
     CDS             complement(70..816)
                     /locus_tag="ACFOA3_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6897391.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(800..979)
                     /locus_tag="ACFOA3_000329"
     CDS             complement(800..979)
                     /locus_tag="ACFOA3_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897392.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(995..1666)
                     /locus_tag="ACFOA3_000330"
     CDS             complement(995..1666)
                     /locus_tag="ACFOA3_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6897393.1"
                     /translation="MQIKKIMKWFPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1735..2406)
                     /gene="rpiA"
                     /locus_tag="ACFOA3_000331"
     CDS             complement(1735..2406)
                     /gene="rpiA"
                     /locus_tag="ACFOA3_000331"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6897394.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2483..2965)
                     /gene="ispF"
                     /locus_tag="ACFOA3_000332"
     CDS             complement(2483..2965)
                     /gene="ispF"
                     /locus_tag="ACFOA3_000332"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6897395.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2997..3686)
                     /gene="ispD"
                     /locus_tag="ACFOA3_000333"
     CDS             complement(2997..3686)
                     /gene="ispD"
                     /locus_tag="ACFOA3_000333"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6897396.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAEGGQISATVDRSG
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3683..4417)
                     /gene="dnaQ"
                     /locus_tag="ACFOA3_000334"
     CDS             complement(3683..4417)
                     /gene="dnaQ"
                     /locus_tag="ACFOA3_000334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6897397.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAEHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4573..5883)
                     /locus_tag="ACFOA3_000335"
     CDS             complement(4573..5883)
                     /locus_tag="ACFOA3_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6897398.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5873..6058)
                     /gene="ccoS"
                     /locus_tag="ACFOA3_000336"
     CDS             complement(5873..6058)
                     /gene="ccoS"
                     /locus_tag="ACFOA3_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6897399.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6058..6357)
                     /locus_tag="ACFOA3_000337"
     CDS             complement(6058..6357)
                     /locus_tag="ACFOA3_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897400.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6708..7904
                     /locus_tag="ACFOA3_000338"
     CDS             6708..7904
                     /locus_tag="ACFOA3_000338"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6897401.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8419..10260)
                     /locus_tag="ACFOA3_000339"
     CDS             complement(8419..10260)
                     /locus_tag="ACFOA3_000339"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6897402.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANCKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10351..10821
                     /locus_tag="ACFOA3_000340"
     CDS             10351..10821
                     /locus_tag="ACFOA3_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6897403.1"
                     /translation="MYDVNTHDVRRFFAHVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(10893..11705)
                     /gene="hpnC"
                     /locus_tag="ACFOA3_000341"
     CDS             complement(10893..11705)
                     /gene="hpnC"
                     /locus_tag="ACFOA3_000341"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6897404.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(11792..12274)
                     /locus_tag="ACFOA3_000342"
     CDS             complement(11792..12274)
                     /locus_tag="ACFOA3_000342"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6897405.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12747..13043
                     /locus_tag="ACFOA3_000343"
     CDS             12747..13043
                     /locus_tag="ACFOA3_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897406.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPATEAPAAEAPAAEAPAAEAPAAEAAATEAPAAEAAASEAPAAEAPAAEAAK"
     gene            13153..14520
                     /locus_tag="ACFOA3_000344"
     CDS             13153..14520
                     /locus_tag="ACFOA3_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6897407.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14701..16629)
                     /locus_tag="ACFOA3_000345"
     CDS             complement(14701..16629)
                     /locus_tag="ACFOA3_000345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6897408.1"
                     /translation="MAADYTYDNSKLTNEQIERLKKLRDRNSEYWEEETYFIKNNGTN
                     SPRTPILFRKDPFDPFENINNSKEISFYDKEYTKDYLVGFAQGFGVEKRNGDTEGPVR
                     EYFKQCLNTGNHTNADCKANPLFSGLYIKSDIFTPWATVKNSHINSEILAVGNYRKFM
                     LAAQLSSHWAEHLYSNSELTLDVQDNSHVIGQTIDLPLLNLTNSLWEPRWDSDVGELK
                     TTNADIRFNTKNESLLVKENYAGGARFRFTYDLKDKVPETPVLTFENNITGTSDIIFE
                     GKALDNLKHLDGHQIVQVNGNADKNAFRLSGKYRKGIYTLSLQQRPEGFFTKVQERDD
                     IAIYAQQAQAANTLFNLRLNDKNSDIFDRTLPRKGLWLRLISGHLSQDVQGKTAPVEG
                     YRKGVQLGGEVFTWQNENNQLSIGLMGGQAEQHSTFRNTDTGNLTTGNMKGFGAGIYA
                     TWHQLQDKQTGAYIDSWVQYQRFRHRIDTEDGTERFTSKSITASIEAGYNALLAEHFT
                     KKGNRLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGFRQLQSRVGVQAKAQFSLYKN
                     IAIEPFAAVNALYHNKPFGVEIDGERRVMNNKTAIESQLGIAVKIKSHLTLQATFNRQ
                     TGKHHHAKQGALNLQWTF"
     gene            complement(17101..17547)
                     /gene="smpB"
                     /locus_tag="ACFOA3_000346"
     CDS             complement(17101..17547)
                     /gene="smpB"
                     /locus_tag="ACFOA3_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6897409.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17602..18612)
                     /gene="waaF"
                     /locus_tag="ACFOA3_000347"
     CDS             complement(17602..18612)
                     /gene="waaF"
                     /locus_tag="ACFOA3_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6897410.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18742..18817)
                     /locus_tag="ACFOA3_000348"
     tRNA            complement(18742..18817)
                     /locus_tag="ACFOA3_000348"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18782..18784),aa:Asn,seq:gtt)
     gene            complement(19008..19760)
                     /locus_tag="ACFOA3_000349"
     CDS             complement(19008..19760)
                     /locus_tag="ACFOA3_000349"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6897411.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20071..20595
                     /locus_tag="ACFOA3_000350"
     CDS             20071..20595
                     /locus_tag="ACFOA3_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6897412.1"
                     /translation="MQTRILSAVLLAFSTTAFAGGAFTLQFDNPSEDGGFTQNQFLSA
                     PYGFGCSGDNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     ATSLQLSRCASIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANMPQSAASA
                     ALCGTSSSASTAAA"
     gene            20556..20735
                     /locus_tag="ACFOA3_000351"
     CDS             20556..20735
                     /locus_tag="ACFOA3_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897413.1"
                     /translation="MRHFFIRQYRSRIIKRRAHTPGIRQLYRIAHCLSRPVLCGEPFY
                     GFLFESNPLRLIEAV"
     gene            20749..20901
                     /locus_tag="ACFOA3_000352"
     CDS             20749..20901
                     /locus_tag="ACFOA3_000352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897414.1"
                     /translation="MTSNVCPPANATASPAAILNSRLAHRSFLLPRRTISTADSYTAT
                     LRYKAV"
     gene            21269..21379
                     /locus_tag="ACFOA3_000353"
                     /pseudo
     CDS             21269..21379
                     /locus_tag="ACFOA3_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21439..22584)
                     /gene="dapE"
                     /locus_tag="ACFOA3_000354"
     CDS             complement(21439..22584)
                     /gene="dapE"
                     /locus_tag="ACFOA3_000354"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6897415.1"
                     /translation="MTETQSLELAKALISRPSVTPDDQNCQKLLAERLHKIGFAAEAL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAENPDHQGSIALLITSDEEGDAQDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMLKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCLGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     ITKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILVRLLAGNAV"
     gene            complement(22684..23304)
                     /locus_tag="ACFOA3_000355"
     CDS             complement(22684..23304)
                     /locus_tag="ACFOA3_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC6897416.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQSEPDR
                     KLQNGEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGA"
     gene            complement(23338..23847)
                     /locus_tag="ACFOA3_000356"
     CDS             complement(23338..23847)
                     /locus_tag="ACFOA3_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6897417.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQITRYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLHGN
                     WDAVAAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGKMFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(23942..24493)
                     /gene="azu"
                     /locus_tag="ACFOA3_000357"
     CDS             complement(23942..24493)
                     /gene="azu"
                     /locus_tag="ACFOA3_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6897418.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMSGKVTLVD"
     gene            complement(24774..25196)
                     /locus_tag="ACFOA3_000358"
     CDS             complement(24774..25196)
                     /locus_tag="ACFOA3_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6897419.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25327..28077
                     /gene="secA"
                     /locus_tag="ACFOA3_000359"
     CDS             25327..28077
                     /gene="secA"
                     /locus_tag="ACFOA3_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6897420.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28221..29993
                     /gene="dnaG"
                     /locus_tag="ACFOA3_000360"
     CDS             28221..29993
                     /gene="dnaG"
                     /locus_tag="ACFOA3_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6897421.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30180..32108
                     /gene="rpoD"
                     /locus_tag="ACFOA3_000361"
     CDS             30180..32108
                     /gene="rpoD"
                     /locus_tag="ACFOA3_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6897422.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         8197 a         9147 c         7582 g         7382 t
ORIGIN      
        1 cgataaattt accacaaaaa agctgcctca aatgaatacc cgggcagctt tttgttgata
       61 tgactccaat cagcggtgtt gcggattgta acgtttttcc aaacgcagga atatccagcc
      121 taagaaagtc gtcatcaaca gataaatcag ggcgacggtg taaagcggtt cttcataaac
      181 cgaataccgg cccgtaatcg tattctgaac atacgccaac tccgccacag caatgaccga
      241 cagcagcgag ctgtctttca agagcgtgat gaactcgctc gccaaaggcg gcagcatgcg
      301 gcgcaatgcc tgcggcagaa tcacatagcg catcgcctgc ggataggtca gccccaaaga
      361 acgcgccgcc tccatctgtc ctttgtctat agactggatg cccgcgcgga aaatctcaca
      421 gatatacgcc cccgagttgg cgatcagtgc caaagaaccg gcaatcagcg gcccgtatcc
      481 gcgacgcagc gcgattgccg cctcgccgct gaccaaaatg ccgtctgaag gatggacgaa
      541 aaacggaaac cacacatacg cccaaatcac aatctgcaca aacagcggcg taccccggaa
      601 cagcgtaaca tacagcagcg aaaccttacg caacgcccac gccagcacgc gcatcggcgc
      661 accggctttt tccaagtgaa tcaggcgcgc caacgccaac aacagaccca ataccgaacc
      721 gcccgccgtc gccacgaccg tcagccccaa ggtcgtcagt gcgccgtaaa ggaacatcca
      781 gcggtattcg taaataatgt caaaacgaaa atccatatac cgtccgtatc aaaaaccggc
      841 ggaactgccg ccgttgcaaa ataatccgcc attttaccgt aaaaaccgcc gcctgaactt
      901 ttttatcgcg gcagacggcg gttgcgcgtc tccgcaaaaa tgcagggcgc gcggttttca
      961 gacggcattt gccgttcaaa gccgtgcggt gtctttacca aatgcccaac cattcgccca
     1021 cggcatccat ccaatcctta ttgcccccgc cgctcctgcc tgctcggcgg tacgcccacg
     1081 gcgcttgcgg atttttagct ttccacaatc ctttgcgttc cctttccgcc tgaatttgag
     1141 cgtcggcata gtcggcaaaa tccgccttat cctgctgttc tttagcataa cttttataat
     1201 gccacgccgc cccgtcctgc acctgcatca ggttcaaatc ggttttgccg acagaaacct
     1261 gcgccacttc gcgctggtag cggtcggtgt cgaacacgcg cacgctgact ttcctgcctt
     1321 ccgccgccgc gcgcaggttg tcgcgcgaac gcgtgccgta agcctgtttc atctccggcg
     1381 cgtcgatata cgccatccgg attttgtgtt tcgcgccgtc gccgtcgata acgtgaaggg
     1441 tgtcgccgtc atagactttg gacaccgtgc ctgtgtagcg gtggccggat ttcgccgatg
     1501 cccgtcggcg ggcgggcgcg tcggaacccg cgtcccctgc cgcgccgagt acgtcgagta
     1561 cggcaaccgc cgtccgcacc gcctcgctgc cgtaccccgt ataacccaac gcacccaaaa
     1621 gcgacagggc gacgggaaac catttcatga tttttttaat ctgcatattt ttcaaatgcc
     1681 gatgccgtct gaacatatcg gaatcggatt tcagacggca tcttaacgtc aggattaccc
     1741 ttggcaggga tagatgactt tcgcaccctc ttccgtcccc aaaatcaaca catcggcggc
     1801 atcgcgggcg aatattccgt tttcgagtac gccggtgatt ttgttgattt cgtcttccat
     1861 cgtcagcggt ttgtcgatat tcaagccgtg gacatcgacg atttggttgc cgtaaaaagt
     1921 ggtgtagccg atacgcaatt caggctgtcc gcccatagcg agcagtttgc gcgaaacaag
     1981 ggaacgcgcg ctttcgacga cttccacggg cagagggaat ttgcccaaac gtgaaacata
     2041 tttgctttca tccgcaatgc agatgaattt ttctgatgcg ctggcgacga ttttttcgtt
     2101 gaggtgcgcg ccgccgccgc ccttaatcat ttgcagggcg tggttcactt catccgcacc
     2161 gtcgatatag accgccaaac ccgatacttc gttcagggaa acgacgggaa tgtcgtactg
     2221 ggcaagcagt tcgctggatt ttttggaggt tgaaaccgcg cctttgattt ttttgccgct
     2281 cttgcccaag gcttcgatga aaaagttgat ggtcgagccg gtaccgatgc cgatgtattc
     2341 gttttcgggt acgaattcga ctgctttttc ggcagcgatg cgcttgagtt cgtcttgtgt
     2401 cgtcatattt ttgtcctttg ggaaaccgta tcaacaaaca gccgccatct taacatttat
     2461 ttgcacgtcc tgcccgccgc gttcaaatcc gtaccagcaa taccgccgcc tgcgcctcta
     2521 tgccttccat gcgcccgaga taaccgagtt tttcgttggt tttacctttg atgttgacac
     2581 acgaaatgtc tatgcccaaa tcggcggcga tgttggcacg catttgcgga atatgcggcg
     2641 cgagtttggg tttctgtgca atcacggtcg tatcgacatt gaccgcctgc caaccctgcg
     2701 cctgaacgct ttgatacgcc gcacgcaaaa ggacgcggct gtccgcatct ttgaactctg
     2761 cggcggtgtc ggggaaatgg ctgccgatat cgcccaaacc tgccgcgcca agcagcgcgt
     2821 cggtaacggc gtgcagcagc gcatcggcat cggagtgtcc gagcagccct ttttcaaatg
     2881 ggatttcaac tccgccaagt atcagctttc tgccttcggt cagttggtgg acatcgtagc
     2941 cctgtccgat acggatgttc gtcatcgttt gtgttcctga tggtttgaat tgaagttcag
     3001 acggcatcga gcagcagcct gacgatgtat gcgtcctgcg gctgcgtcag tttcaaattg
     3061 cgcgcgtcgc cctgcaccag caaagggcgg atgcccaatt tttccacggc ggacgcttca
     3121 tcggtaatgc cgtccaagtt ttccgcagcc aatgcgcggt gcagcagccc ggcgcggaaa
     3181 agctgcggcg tttgcgcctg ccaaagcccc gaacggtcga cagttgcact gatttgtccg
     3241 ccttctgcgc gtttgagcgt atcggcaacg ggaactgcca aaattccgcc ttcggcagcg
     3301 ttgcccgcct gttctatcaa ccgcgtcaga gcttcagacg gcaggcagca tcgggcggca
     3361 tcgtgtacca aaatattgtc ggtttccgcc gccaaaccgg tttccaacaa ttttgccaca
     3421 ccgtttcgga cggtttcggc gcgggtctgt ccgccgtttt tccacacccg aacctgtgga
     3481 aatgccgtct gaaccttatc ggcaaacgtg tcttcgggcg agacgacaac gacggtcaaa
     3541 tcgacgccct catgccgttc aaaaatccca agcgtatgtt ctaaaacggt tttgcttccg
     3601 atttcgacat attgcttggg tttgtccgca ccgaaacgcg ccccgatgcc ggcggcggga
     3661 atcagcgcga tatttttgcg cttcatgcgt ccgtcccgcc gttttcagac ggcacggctt
     3721 ccttgcgcca gatacaggct tcgcccaagc cgtccaaata ttgcccgtgt tccgccaact
     3781 cgttttcgtc cgccctgatg actttcagtt tgccgctgcg tttggtttcg gtatgcacca
     3841 cgggtttggt ttccattttt tcctctgcgg ccgcacccat caggtcgaac tgccgccgcg
     3901 tcatagcaag atagacttcg cccaaaagtt cgcagtcgat caatgcgccg tgcaggacgc
     3961 gcttgctgcg gtcgacggaa aaacggttgc acaaggcatc caggctggct ttctgcccgg
     4021 ggaacatttc gcgcgccatc gccagggtat cggtaacggt acagccgagt tcctcaacgg
     4081 tcggcaaccc catccggcgg aactccatat tgaggaagcc cacgtcgaat ttggcattgt
     4141 ggataatcag ttccgcaccg cgcaggaaat cggcaatctg cctgccgacc tctgcaaacg
     4201 gcggcgcgtt tttcccttcc aaaacctcta tcgtcagacc gtgcaccctc gccgcctctt
     4261 cgggcatatc gcgctcgggg tggacataga ggtgcaggtt tttgtcggtc atttggcggt
     4321 tgaccatttc taaaccggca aactcgacca agcggtcgcc gccgtcggca tacagaccgg
     4381 tggtttcggt atcgaggatg atttggcgtg tcgtcatatc ggtgtctttc ttctatcttc
     4441 gtaaattgct tattttttaa gcaatgtatt tttctgtttt catttcaatg cacaaaccca
     4501 cttattcaca gtgtgttcac aacattgggc aggcggattg tgtattttgg ggacaatttt
     4561 ttcagacggc attcaaggtt ttttcctgat tgccgccgcg cctaaaaacc gcctttcgcg
     4621 cttaatcaaa aataccgaca acggaatatt gcccaaagcg acaatcagat acaacaagga
     4681 aatgctgtca aacaaaaaca gcaacaccgc gctcaaaacg gcggcggaaa ccataaaaat
     4741 accgttaacg atattgttgg cggcaacggc gcgggcgcgg aaagtctcgc tactggcggt
     4801 ttgcagccaa gtatagagcg gaacggagaa aaatccgccg aaaaagccga tcagcgtcat
     4861 caccgccatc acgggatatg cccatccttg cgataaaaac caaaaaatgc cgttcagccc
     4921 ttcaaaacgg tgtccgtgcg tcagccacac caaaaccagg ccgcaaaccg tcaaacccaa
     4981 cgcaccaacc gttacccaag ccaacatcag ccgttccctg ctgaacttgg cacacagtac
     5041 cgaaccggcg gcaataccga tggaaaacag ggcaagcatc aggttgaaaa cattgtcgtt
     5101 gccgcccaga tggatttggg taaaggtcgg cagttgcgtg gtataaaccg cgccgacaaa
     5161 ccaaaaccac gaaataccga taatggcggt aaaaacgggc ttgtgccgca ccgtttcact
     5221 cagcagggat tttgtgccac ggacaatatt ccactcaatt tgtgtatcgg cagccttggc
     5281 gggtacagac ggcataaaca ggctgccgac cgtgcctccg acggcgacca gcaaaaccag
     5341 tatcccgaca atataaggcg gtacacctgc caccgccgtt cccaaaatct gaccgaacag
     5401 gatggcgaca aacgtacccg attcaatcag gctgttgccc atcatcaact ctttgtcgtc
     5461 gagataatcg ggcaggatgg cgtatttcag cggcccgaac agcgtcgatt gcgcgcccat
     5521 gcaaaacaga cacgccaaaa gcagcggggc agaccggata taaaacccgt atgccgccac
     5581 cgccataatg atcatttcca gcaccttgac ccaacgcgcc aaaacggcct tgtcgaattt
     5641 gttacccaac tgccccgaca gcgaggaaaa caggaaatac ggcaaaataa acagcaacgc
     5701 gcccaagttc aacatctgtc cggcaggcag gaagccgttt tgccccaaac cgtaaaaccc
     5761 aatcatcaca aacagcgcgg ttttgaacac attgtcgttg aacgcgccga gaaactgcgt
     5821 agcgaaaaga ggtgcgaaac ggcggctttt aaccagtccc aaaccgcctt ttttagcgta
     5881 catcgttttc cctctctttt tcaatcagtt tacttgtcga atcatcatcc atcaggatgc
     5941 ggtgcgccgg cccttccaag tcgtcaaact gcccgttttt gcccgaccac caaaaaaacc
     6001 agccgatgac aaacgccaaa ataatgctga tgggcaccaa tataaacatg ctttccatca
     6061 catattccct gtcaaatcgt tcaaaacaaa agtctgcccc gatacggtca gatattcgtt
     6121 acgcaaagtt ccgacgggag cttcgtcaaa aaacagctcg atacggtctt tgaccacgcg
     6181 ccaatattgg gggatttccg tctgaccgaa cggcgacagg acatgatttt ccattccgcc
     6241 ttcaagtttg acggcaaaac gcccgctttg cggccgtgct tccgattcgt cgtcggcaag
     6301 caggatgaaa aagcctatat gccgtcccga ttggtcatga atactgaaat aatgcataaa
     6361 tttcccaccc gccttttttc agacgacacc aactaaaaac agggcgaatg taccagtttg
     6421 gacgggaaga atgcaaagaa attctccctc ccccagccga aaacaccggc aaaccgcata
     6481 tccccctttt ttccgtcaaa atgcccgact tccgccattt tcacgcaaac gcccgattaa
     6541 gccaagcaat tgcaaagatt ttttgctaga atagcctgct tcttttatca accttttcag
     6601 acggccccac tactttcccg cccaggaagg caaaacggat tcggcacgaa tccggttagt
     6661 atccgtgtcc gattccaatg ccgtctgaaa ctttccggag taagaaaatg tcccaaaaat
     6721 tgatcttggt tttgaactgc ggcagctcgt ccctcaaagg cgcggtcctg gataacggca
     6781 gcggcgaagt cctgctcagc tgccttgccg aaaaactcaa cctgcccgat gcctacatca
     6841 cattcaaagt aaacggcgaa aaacacaaag tcgatctgtc cgcacatccc gaccacaccg
     6901 gcgcggtcga agccctgatg gaagaactca aagcccacgg cctcgacagc cgcatcggcg
     6961 ccatcggcca ccgcgtcgtc agcggcggcg aactgtacag cgaatccatc ctcgttgacg
     7021 acgaagtcat tgccggcatc gaaaaatgca tcccgctcgc ccccctgcac aaccccgccc
     7081 acctcttggg cctgcgtgcc gcgcaaagca ttttcaaaga cctgcccaac gtcgtcgtat
     7141 tcgatacctc cttccaccaa accatgcccg aagtcgccta caaatacgcc gttccgcagg
     7201 agttgtatga aaaatacggc ctgcgccgtt acggcgcgca cggtaccagc taccgcttcg
     7261 tcgccgacga aaccgcgcgc ttcctcggca aagacaaaaa agacctgcgt atggtcattg
     7321 cccacttggg caacggcgcg tccattaccg ccgtcgccaa cggcgaatcg cgcgacacca
     7381 gtatgggcct gaccccgctg gaagggctgg taatgggtac gcgcagcggc gacatcgatc
     7441 cttccgtatt cggcttcctc gccgaaaacg ccaatatgac catcgcccaa atcactgaaa
     7501 tgctgaacaa aaaatccggt ctgctcggca tttccggcct gtccaacgac tgccgcacca
     7561 ttgaagaaga agccgccaag gggcataaag gcgcgaaatt ggccttggat atgtttatct
     7621 accgccttgc caaatacatc ggcagtatgg cggttgccgc aggcggtttg gacgcactgg
     7681 tctttaccgg cggcatcggc gaaaactccg acatcatccg cgaacgcgtg atcggctact
     7741 tgggcttcct cggtctgaac atcgaccaag aagccaacct gaaggcacgc tttggcaacg
     7801 ccggcgtgat taccactgcc ggcagcaaag ccgttgccgt ggtcattccg accaacgaag
     7861 agctgatgat tgcccacgac actgcccgtt tgagcggctt gtaaggtttt atccgcacac
     7921 gaactgcctc tggaaacgga ggcagttttt ttatccggct ttccatgctt aaacagcact
     7981 gcctcttttc agacattgac ggttgcagcc gcttacctga accttatagt ggattaaatt
     8041 taaatcagta cggcgttgcc tcgccttgcc gtactatctg tactgtctgc ggcttcgtcg
     8101 ccttgtcctg atttaaattt aatccactat aatgattgac tattttttaa tcatgttatt
     8161 attttccata aaatacatga cattaagatg tttttccaca aaagatacac acaccggcaa
     8221 acaccggctg tgtttatctt ttcttatgcc tattttttaa tcatcgtatt tttatctttt
     8281 aatttcaata cgcaaactaa cttatacaca cggttttcac atctttggac tgcttccgtg
     8341 tgtatagcgg atattgccgt tttcctttct gacaaaaatg ccgtctgaga acttcagacg
     8401 gcatttgaaa catcggaatc agcggttttg ttcataccac tcgataaact tgtctgcttt
     8461 gacaaaaccc agcagcggct cgctgcggct gccgtcggag cggacgacaa acacgcccgg
     8521 cggcccgaac agaccgtatt ctttcaacaa cgcctgatgt tcgggcgtgt tggcggttac
     8581 gtcgatttgg aaaaagcgtt ccatatcgac tgcctgatgc acttccggct gattaagcgt
     8641 gtaagccgcc atttctttgc aggaaatgca ccagtcggca taaaaatcca aaacgacggg
     8701 tttgtcggga tgttctttca acgccgtatc catcgctgcc ttcagcgcgg cagtatcggc
     8761 aaacattttt ccgtgttccg aagatttgcc tgcttcggcc ggcggattga gggtcaggaa
     8821 atggtgcagc gcggtcgttt tgcagtttgc gccctgccag ccgaaccacg cgccgcctat
     8881 cagcaatatg ccgcccaatg cgaatgccac agctttcgga cggcgtttct gcctgcgtcc
     8941 gttgaccagc agcatacagg caggaaccag catcagcagc gtgtacagcg cgacgacgag
     9001 ataatagggc aagtgcggcg tggcgaggta aacggcgacg gcaagcagga taaagccgaa
     9061 agcgtatttg acggcattca tccaatcgcc tgccttaggc aggatatgcc cgccgaacgt
     9121 gccgatggca atcagcggaa cgccggtgcc caacgccaaa gtgtaaagtg ccaaaccgcc
     9181 taaaaccgca tcgcccgtct gaccgatgta gcccaaagca aatgccagcg gcggggcgac
     9241 gcacggcccg acaatcagcg cggacaatat gcccataata aagacggaaa cgattttacc
     9301 gcctgaaagc ctgctgcttt gattctgaaa atacgactgc acggcgttgg gaagctggat
     9361 gttgaacagc ccgaacatag acagtgccaa gacgaccatc aaagccgatg ccgccaacac
     9421 gacccaagcc tgctgcaacc atacggtcag cagcgcgccc gtcagtccgg caacaatgcc
     9481 gaccagcgta taagtcagag ccaaaccctg aacataaacg acggacagca caaacgcccg
     9541 cgccttgccc gcctttttgt cgccgaccac aatactggaa acaatcggca acaggggata
     9601 catacaggcg gtaaaactca gacccaaacc ggcgagaaaa aacgccaaaa gattggcgtt
     9661 gagcgtatcc caagacagct tgaaacggct gtcgccgccc tcatccccct tcgggggcgg
     9721 cagcgccccg ctgccgtttt gagaggaagg ctgcaaaaag cgatctttgg cggatgccgg
     9781 ttcgtcggtt tgcggatggt aagtgccgtt gccggaaata tcaaactcgg tatccacggg
     9841 cggatagcac acgccggctt cggcacagcc ctgataggtc aaaaccaatt tatacggttc
     9901 gccgacagcc tttgcataag gaaaggcaac ctgcgcttcg tgatggtaaa ccgtctgcct
     9961 gccgaaaaac tcgtcttcct tctcttcgcc cttgctgaaa gaaggctgtc ccaacaaatc
    10021 cgccggatcg gtcttgccga cgattttcgc ctgatacata tagtatccgt cggcaatcct
    10081 gaaacggacg ttcacaccgt cgtcggcaac ggcaagctcc ggcacgaatg ccttttccgg
    10141 cggcagcaga tcgttcgcat ccagcgcgaa agctcgtccg cacaacatca aaaatacggc
    10201 gaacaggcaa atcagttttt tcataatcga atccgtttca gacaaataat ttgtctgcat
    10261 tataaatggt aaggttgacg gtgggattta atttatgtaa aacccgccat tatccgaacc
    10321 tatttccata aacatcttat cgaacccgcc atgtacgatg tcaataccca cgatgtccgc
    10381 cgctttttcg cccacgtgtg gcagcagcgg ctcaatccgc tgcaactgag cgcactggaa
    10441 cagaaagccc tccgcattgt cgaagcccat cccgaatacc accgctatct cgaacgcatc
    10501 gaagaccatc tggacaccga ctggctgccc gaaaacggcg aaagcaaccc cttcctgcat
    10561 atgtcgctgc atctgtccgt ccaagaacag gcgggcatag accagccgca cggcatacgc
    10621 gccatccacg acaccctgtg cgccaaacgc ggctggctgg aagccgaaca cgaaatgatg
    10681 gaggcactgg cggaaacact gtggacggcg caacgctacg gcaccggttt ggatgtcaat
    10741 ttctacatga cccgactgcg caaactcatc ggcttgggtg cagaaaatca agccaggttg
    10801 aacccgcatg aaatcgcctg accataccaa ccgcctgcaa aatgccatct gaagcggaac
    10861 aacccctttc agacggcatg catttccccc aatcatttcc acaacgcctt tttcagcata
    10921 atcaaccaat ccttcttatc caaaacgggg cgttgtgcaa acacatcgta tcggcacgcg
    10981 tccagtttct gcaaaatcaa ctgcgccccc aacacaatca tacggagttc caaaccgata
    11041 cgccccttca gttcccttgc caaaggcgaa cccgccttca gcatacggaa tgcgcgtcgg
    11101 cactcatacg ccatcagccg ctgaaacgcc gcatccgccc gtcctgccgc gatctgttcc
    11161 tcagaaacac cgaatttcaa caaatcgtcc tgcgggatat aaaccctgcc cttttgccaa
    11221 tccacagcca catcctgcca gaaattgacc agttgcaaag ccgtacaaat gccgtcgctt
    11281 tgcgccacgc acaccgcatc cgttttcccg tacaaagcca gcataatgcg tccgacaggg
    11341 ttggcggaac gccgacaata atcggccagc tcgccgaaat ttccatacct tgttttaacc
    11401 acatcctgag aaaatgcaga aagcaaatca taaaacggct gcaaatccaa accgaacggc
    11461 acaaccgcct cggcatccaa tcgtgcaatc aaaggatgcg ccgaccggcc gcccgatgcc
    11521 aacacgtcca actcgcgctg caaaccctcc aaccccgcca acctggcttc agacggcata
    11581 ctgccctcgt ccgccatatc gtccgccgtc cgtgcaaacg cgtacaccgc gtgaaccggc
    11641 ttcctcaacc tgcgcggcaa aatcagcgaa ccgacgggaa aattttcata atgcccaacc
    11701 gacatacctt ctccatccat caaacaaaat gccgtctgaa atggaacaaa cccttttcag
    11761 acggcatcag atacctccaa gctgccggca atcagtggtg gtgatgaccg tgcggaccgt
    11821 ggacatgacc gtgtgcgatt tcctcatcgg atgcatcgcg cacactttca accgtagcct
    11881 taaagcggat tttcatgcct gccaaaggat ggttgccgtc caccaccgcc ttgccgtcgg
    11941 caacatcggt tacacgatag acgacaacat cgccggtttc aggatcgtcg gcttcaaaca
    12001 tcatgccgac ttcgacttca acagggaaca cgcccgcatc ttcaatacgg accaactccg
    12061 gatcctgctc gccgaacgca tcgtcgggcg acagcgccac atcgaccgta tcgccggcat
    12121 ccttaccgtg caacgcctct tccaccaaag ggaaaatgcc gtcgtaaccg ccgtgcagat
    12181 acgcaatcgg ttcttcggtt ttgtccaaaa gctgattgtt ggcatcatac atctcataat
    12241 gcagcgaaac cacggaattt ttcacgatag ccatatttgt cctttcagga acagcagatt
    12301 gattacaggc gcattctaac acaaccgccg cgtcggccga ttaccgttaa cctgttcata
    12361 aactgtacag cacatatttc aatgtaaatt tttgttattt tattgcggtg taactttttt
    12421 acaacattct taaaaccatt ccgacctgtc tgccgacttt cccaatccgc cttaataaat
    12481 catacaagat actgaaatta tattaatctc tataatattt atccctatcg aatttttaac
    12541 agcaaaaccg ttttacagga tttatcaatc cgcccgccag aaaacttttc attcaaacct
    12601 ttttcccatc tgtacgacat tgcaatccct tattccatag tgcataatta cgcaaattca
    12661 gcgatgaatt tccaacccgg tttgtagtat ggtcgataaa gacctatttg tttcaataat
    12721 ttaaattggt tctaaaggtt actaaaatga aaaaatccct gtttgccgct gctttgttgt
    12781 ctttggtcct ggcagcctgc ggcggtgaaa aagccgctga agctcccgct accgaagcac
    12841 ctgccgctga agctcccgct accgaagcac ctgccgccga agctcccgct actgaagcac
    12901 ctgccgccga agctcccgct gctgaagcac ctgccgccga agctcccgct gctgaagctg
    12961 ccgctaccga agcacctgcc gctgaagctg ccgcttctga ggctcctgcc gctgaagctc
    13021 ctgctgccga agctgcaaaa taagcatttt ccgcttgcaa aaaagcagga tacgttcagt
    13081 atcctgcttt tttgattttt cagacggcat cagattccct tcctcaatct tctccctacc
    13141 cttccgacaa acatgcctga ccttcatacc gaattttccc gactcctgcc ggcagatgaa
    13201 attgccgaac tttctccgac gcttttaaaa gaccagcgca accgctttac gtctgcacca
    13261 gacatcattt tgcagccgcg cagcgttgaa agcgtgcaaa ccattatgcg tttttgttac
    13321 gaacaccgta ttccggttac gccgcaaggc ggcaataccg gtttgtgcgg cgcggcagta
    13381 tcggaaaacg gcgtattgct gaacctttcc aaactcaacc gcatccgcag catcaatttg
    13441 tcagacaact gcataaccgt cgaagcaggt tccgtactcc aaaccgtcca acaggcagcc
    13501 gaagcctcaa acaggctgtt cccactcagt ctcgccagcg aaggctcctg tcaaatcggc
    13561 ggcaacatcg cctgcaatgc cggaggtttg aacgtattgc gttacggcac gatgcgcgac
    13621 ctgattatcg gtttggaagt cgtcctcccc aacggcgagc tggtttccca tctccatccc
    13681 ctgcataaaa acaccaccgg ctacgacctg cgccatctgt ttatcggcag cgaaggcaca
    13741 ctgggcatca tcactgccgc cacgctcaag ctttttgccc gtccctcgga caaggcaacg
    13801 gcatgggtcg gcatacccga catcgaatcc gccgtccgcc tgctgaccga aacccaagct
    13861 cactttgccg aacgcctatg cagttttgaa ctggtcggcc gttttgccgc cgagttgtct
    13921 tccgaattca gcaaattccc cctgccgaca cattcagaat ggcatatttt acttgagttg
    13981 accgactcat tacccgacag caatcttgat gatcggcttg tcgaatttct ttataaaaaa
    14041 ggatttaccg acagcgtatt ggcacaaagc gaacaagaac gtatccatat gtgggcattg
    14101 cgcgaaaaca tctccgcatc gcaacgcaaa ctgggcacca gcatcaaaca cgatattgcc
    14161 attcctatcg gacgcgttgc cgactttgtc cgccggtgcg ccaaagattt ggaacagaat
    14221 tttaaaggca tacaaatcgt ctgcttcgga catcttggcg acggcagcct gcactacaat
    14281 actttcctgc ccgaaatcct cagcaatgaa gtctatcgtt acgaaaacga catcaacgac
    14341 gcagtttatc gcaacgttct tgcctgcaac ggcacgattg ccgccgaaca cggcataggt
    14401 atcatcaaaa aacagtggct ggacaaagta cgcacgcctg ccgaaatcgc cctgatgaaa
    14461 agcatcaaac aacaccttga tccatataac attatgaatc cgggcaaact gcttccgtaa
    14521 ccggcatttc tgatttgcat acacaacaaa gaaagggaca atagatccga ttgtcggttt
    14581 agcgcgagct cgtgagtgcg gtaaaaaatt ggtggaaatt acacgaaaaa tgaccgcact
    14641 tttaaaataa aaaaatcggc agtaaatttc cctgccgatt ttattttgtt acaacttaac
    14701 ttaaaacgtc cactgtaaat tcagcgcacc ttgtttagcg tggtgatgtt tacctgtttg
    14761 gcggttgaat gtcgcttgta aggttaagtg agatttgatt ttcactgcta tacctaattg
    14821 actttcaatt gccgtcttat tgttcatcac tcgacgctct ccgtctattt ccacaccgaa
    14881 aggtttgttg tggtaaagcg cgttcacagc ggcgaaaggt tcaatagcga tatttttata
    14941 gagtgaaaat tgagctttag cttgaacgcc cactcggctt tgtaattggc ggaagccaag
    15001 taaattcacg tgggcatttt cgctatcgct gaattttccg tttaccccca aataagtcaa
    15061 ttgtgcctgt ggttgcaggt aaacacgaag tctgttgccc tttttagtga agtgttccgc
    15121 caataaagcg ttgtaacctg cttcaattga ggcagtaatg ctttttgaag taaaacgttc
    15181 tgtaccatct tcagtgtcaa tacggtgacg gaaacgttga tattgtaccc agctgtcaat
    15241 atatgcccct gtttgtttat cttgaagttg atgccaagtg gcataaatgc ccgcaccaaa
    15301 gcctttcata tttcctgttg taagattgcc tgtatctgtg ttacggaaag tgctgtgttg
    15361 ttctgcttgt ccgcccatta agccaataga aagttgatta ttttcgtttt gccatgtgaa
    15421 tacttcgccg ccgagttgca cgccttttcg atagccttct acaggcgcag tcttaccttg
    15481 tacatcttgg cttaagtgac cactaattaa acgcaaccac aagcctttgc gtggtaaagt
    15541 gcggtcgaaa atatcgctgt ttttgtcgtt caaacgtagg ttgaataaag tattggcggc
    15601 ttgagcctgt tgtgcataaa tcgcaatatc atcgcgttct tgcactttgg taaaaaagcc
    15661 ctctgggcgt tgttgtaaag aaagcgtata aattcctttt cggtatttac cagaaagtct
    15721 aaatgcattt ttatctgcgt tgccatttac ttgaacaatt tgatgtccgt ctaggtgttt
    15781 caaattatcc aacgcttttc cttcaaaaat aatatctgat gtgccagtta tattgttttc
    15841 aaaagttaaa actggtgttt cagggacttt atcttttaga tcgtaagtaa aacgaaaacg
    15901 agctccgcca gcataatttt cttttacgag taaactttca tttttagtat taaaacggat
    15961 gtctgcattc gttgttttta actccccaac atcagaatcc caacggggtt cccagagaga
    16021 atttgttaaa tttaataatg gcaaatcaat cgtttgtccg ataacgtgtg agttatcttg
    16081 aacatcaaga gttaattctg aattggaata gagatgttct gcccaatgag aagaaagttg
    16141 ggctgccaac ataaattttc tataattacc gacagccaaa atttcgctgt tgatatggct
    16201 gttttttact gttgcccaag gggtgaaaat atcgcttttt atgtataagc cagaaaataa
    16261 tggattagcc ttacaatcgg cattggtatg gttcccagta tttaaacatt gcttaaaata
    16321 ttctcgtacc ggtccttctg tatccccatt tcttttttca accccgaaac cttgggcaaa
    16381 accaacaagg taatctttag tgtattcctt atcataaaaa gaaatctctt ttgagttatt
    16441 gatgttttcg aatggatcga aaggatcttt acggaataat attggagttc ttggagagtt
    16501 tgtgccgtta ttttttatga aataagtttc ttcttcccaa tattcactat ttctatcgcg
    16561 gagttttttt aatcgttcaa tttgctcatt tgttaattta gaattgtcat aagtataatc
    16621 agcagccaaa agcggagagg tataaagaat agaaaaaaat agacttacaa taaatgattt
    16681 tttaaacttc tgcttgcttg cttgcttgct tgcttgcttc gagtttcata ataaattttc
    16741 ctttgtcaag taaaaataaa tagggcgtgg attttagcat aaaactgaac aaaaaatgtc
    16801 atttagttca aattttctct atttatttct tatttattaa aagtaaacgt ttgctttttg
    16861 ctattttgtc aagccagttt gaaaatgtgt ataattgccc tcgttattca caaaaatttc
    16921 aggaaaaatg accgcacttt acccttggtt aatgccaatt tatcatcaaa ttgctcaaac
    16981 ctttgacgaa ggtttggggc atcatgctgt gctgattaaa gcggattctg gtttaggtgt
    17041 agaacgttta cacatcaggc ggcagccttg cccataccgt ctgaagcact gtttccacaa
    17101 tcagcgcgta tgcttaatca accgctgttt ctcgcgtttc caatccgcct ctttcatact
    17161 ctgacgtttg tcgtgctgtt tcttaccttt tgccaaaccg atttccatct tgatttttcc
    17221 gcgcgaaaaa tgcaaatcca gcggcacgat ggtgtagccg gcacgttcgg ttttgccgat
    17281 taatttgttg atttccgact ggttcaacaa gagcttgcgc ggacgtacgg catctggttt
    17341 aatgtgtgtc gaggctgtgg gcaaagccgt aatatggcag ccgaccagat aaaacgcgtc
    17401 ttttttccaa tagatataac tctctttaag ctgtacgcgc gcggcgcgga ttgctttgac
    17461 ttcccagcct tccaagacca aaccggcttc aatccggtct tcaatgaaaa aatcgtgaaa
    17521 tgctttttta ttgttcgcaa tagccataaa catcctatca atatccgccg tcagacggca
    17581 taaacccgaa aacagaaccc atcataccgc ctcttcaacc gcctgcacaa tcttctcggg
    17641 atacagcctg ttgaggcagt cggtatgccc cagcggacat tcccgcttaa aacagggcga
    17701 acattccaag tgcaggctga cgattttcgc cctgtcgctc aaaggcggcg tatgcgtcgg
    17761 gctggaagaa ccgtaaaccg ccaccacctt cctgcccaat gccgccgcca agtgcatcaa
    17821 tccgctgtcg ttgcacacaa ccgtgtccgc caacgacagc aaatccattg cctgcgacaa
    17881 atcggttttg ccgcacaaat tgacacacat accgtctgaa aggcagttga tttcctcggc
    17941 aatttcatca tctttttgcg aaccgaacag ccaaacctgc caacccgccg ccagataatg
    18001 tttgcccaac tcggcaaaat gccttgtcgg ccaacgcttt gccggcccga attccgcgcc
    18061 cgggcaaaat gccaaaacag gctttccaag attcaaaccg aaagtttcga cagaaatttc
    18121 ccgccgccgt tcatcaatgg aaaactcggg gaatcccgaa tgcccgtcaa aatcttcctg
    18181 actcggatgc gcgagagccg tatatctgtc caccatcaaa ggcagacgtt ccttatccag
    18241 cctgcgtata tcgttcaaca gaaaataacg gctttcaccg acataacccg tccttttggc
    18301 aatacccgtt gctagcgcga tgactgccga tttcaaagaa ccgggcaaca cgataacctg
    18361 atcgtatccg cgccgcccca aatccctgcc gacccgccag cggcgtttca actccaacgc
    18421 accgtgtccg aacggattct caagaatttc attcacttcc ggcatacgct cgaacaccgc
    18481 catcgaccac ttcggcgcga acacatcaat cgtgcaaccg gggtgaagtt ccttcaaacg
    18541 gcggaacaag ggctgggtca tcacgcagtc gcctatccaa ctgggagaaa taatcaggat
    18601 tttgatggac ataacaagaa accgaaatca gacaggcgga attttaccgc gaaaccgttg
    18661 gaaaacctat ctcgccgcat tccgaacgcc ggacgtgcaa atatgaaaag cccgaacatt
    18721 caagttcggg cttcaaaatt ctggctcccc gacctgggct cgaaccaggg acctgcggat
    18781 taacagtccg tcgctctacc gactgagcta tcggggaatg gggcgtatta tagcgtccgg
    18841 aaaaaatgtg tcaatcctta attttggaaa aatgggcgac aaaacgacaa gcatatgaat
    18901 cagaaagaca ttaagaccga tgccttaaaa ggattgccgt tgtatgaatt tccacagccg
    18961 tcatcacacc atatttaagc ccgatgagcc gttctgccct cccccgctta aaacaatgcc
    19021 gtctgaactt cgccgcgttc caaagccagt aaaaactgtt tgcggttcaa cccgcccgcg
    19081 tagccggtca gtttgccgtc gctgccgatg acgcggtggc agggaatcag gatagatacc
    19141 ttgttctgcc cgttggcggc ggcaacggcg cggacggctt tggggttgcc caaacgctgc
    19201 gcctgctcct tgtagctgcg cgtttcgccg taaggaatcg ccaagagcgc gtcccatgcc
    19261 tgcttttgaa actcggtgcc aatctgctcc aaaggcgtgg caaaggtttt cagacgaccc
    19321 ttgaagtata agtccaattc ctgccgcaaa agttgcgtcc gctcatcctc ccgaaacaca
    19381 aaccgtccgc gcaaggcttt ttggacggcg gcaatttcct gttccaaatg cttctgtccg
    19441 acaaactcca gcagacacaa tcccttattt ccgaacaccg ccagcatctc gcccaaaggc
    19501 gtggcaatgg cggcacacac cagctcgttc aaactgtcag gataacgcgc ttccaacaaa
    19561 cggatggcgc ggcggatgcg gacatattct tcaggcgcgc agccgatatt gtcccaaaaa
    19621 tcccgatcga actgcccggc ttcgcattcc gtcaaattgg gatgcggcat aacgccgcac
    19681 tcaaaaaccc gggtttcgag gcaatggcgg atttcatccc attttgacgg caggttgttt
    19741 aaggaaggca gggtaatcat tcgtttgctc cgtatctcta tcatggattt ggcggcaaaa
    19801 tccccgattt ccgccattcc cgcacgccga tgcaggaacg ggctatgacg taaatcttga
    19861 gggttgggtt gcggcaatgc ctaaatatgc gatatttcta aagcctcagg gaaaggaatt
    19921 aaagacttca gacgacctgc aaagggtcgt ctgaaaccac gatttttgca tttgcgcatt
    19981 tcggcacatc atccaaccgt ttcggcacat tcctgccgcc gttgacagcc tataatgaat
    20041 ccacttattc atcaagcaaa ggaatcatct atgcaaaccc gcatcctctc cgccgtactg
    20101 ctggcttttt caaccactgc ctttgccggg ggcgcattca cgctgcaatt cgacaacccg
    20161 tccgaagacg gcggcttcac gcaaaaccag tttttgagcg cgccttacgg cttcggctgt
    20221 tcaggcgaca atgcttcgcc cgcgctgtcg tggaaaaatc cgcccgccgg gacaaaaagt
    20281 ttcgtcctga ccgtttacga taaagacgcg ccgaccggac tgggctggat gcactgggtg
    20341 gtcgccgaca ttcccgccga tgtccgccgc cgcaacgcga cctcgttgca attaagccgc
    20401 tgcgccagca tcgccgacga ccagtccgca gccatatcgg cggtaatcag tttgcggatt
    20461 tgccgcatca ggttgacgcc ttcgtacacg gcaaaaccga tgccgtcatg ctgcaaccac
    20521 gccaacatgc cgcaaagcgc ggcctccgca gcattgtgcg gcacttcttc atccgccagt
    20581 accgcagccg cataatcaaa cgccgcgccc atacgcccgg aatacggcag ctttaccgca
    20641 tcgcacactg cctgagccgt cccgtattgt gcggcgaacc tttctacggt ttcctgttcg
    20701 aaagcaatcc attgcgcctg atagaggccg tctgaatcgg gaatattgat gacgtcaaac
    20761 gtctgcccgc ctgccaacgc aaccgcctca cccgccgcaa tcctcaattc gcgcctcgcc
    20821 caccgcagct tcttgcttcc gcgccgcacg ataagcacag ccgattcata taccgccacg
    20881 ctgcggtaca aggcggtatg atgttgcacg atgccgccta aagcacccaa tcgttcgcgt
    20941 gtatgaaagt aatttgacgg cactattatc tccaacacgc agggccgcac ataaggcgca
    21001 gccctgttta ccctgatttg gaaggggtta cttcccaaaa ataaaccaaa atcccactgc
    21061 cccgaatggc cgggtttcaa ccatattccg tcggtgtgcc tgcaacttga ctccctcccc
    21121 tgtgggggag ggttggggag agggtaaaac ggggcagata cagacaatat ttccgttacc
    21181 gccccgatgc cctctcccta gccctctccc acgaaagagg gaatggattg ccgtttaaat
    21241 aaaaaaatct acataaaaat caatgtgtta tagtggatta aatttaaatc aggacaaggc
    21301 gacgaagccg cagacagtac aaatagtacg gcaaggcgag gcaacgccgt actggtttaa
    21361 atttaatcca ctatatctca aacccacgtt aggtctaagc aaatggtcgg acatccgtat
    21421 ccggcaagca agctgctttc agacggcatt tccagccaac aaacgcacca atatcccctc
    21481 atacaccgcc gacagcttcg gaatgtcgtt tagccgcacg ttttcgttga tttggtggat
    21541 ggtggcgttg gacggaccta attcgataag ctctttcgta atggctttaa tgaagcgtcc
    21601 gtccgaagtt ccgcctgtgg tggacaattc ggtctctacg ccgcagattt cggcaatggc
    21661 ggcgcgtgcc acgtcggtca gtttgcccgc gtgggtcagg aagggctgcc ccaaacacga
    21721 ccactgcaaa tcgtattgca caccgtgttt gtccaaaatg gcgtggacgc gttgtttcag
    21781 ccctgcttcg gtggactcgg tggagaagcg gaaattgaat ttgacattca gctcgcccgg
    21841 aatgacgttg gtcgcgcctg tgccgccgtt gatattggaa atttgaaagc tggtcggcgg
    21901 gaagtattcg ttgccttcgt cccagacttc ctgtgtcagc tctaacaagg caggggcaaa
    21961 agtgtggacg gggttgattg ccaaatgcgg ataggcaata tgcccttgct tgcctttgac
    22021 ggtcaggttg cctgacagcg aaccgcgccg gccgtttttc aacatatcgc ccaatttatc
    22081 cacggcagtc ggttcgccga cgatgcagta atcgatcaac tcgccgcgcg ctttcaacac
    22141 atcgacgact tttgttgtgc cgtcctgcgc atcgccctct tcgtccgaag taatcaggag
    22201 cgcaatgctg ccttgatgat cgggattttc ggcaacgaaa cgctcacagg cggtaacaaa
    22261 acaggcgatg ctggttttca tgtccgccgc gccgcgcccg tataatcttc cgtcgcgttc
    22321 ggtcggctcg aacgggggcg aatcccattt ttcgacaggg cctgtcggca cgacgtcagt
    22381 atgccctgca aaacagacga cgggagcttt cgtgccgcgt cgcaaccaga tgtttttggt
    22441 atcgccgaaa tggagtgcct cagccgcaaa accgattttg tgcaggcgtt cggcaaggag
    22501 tttttggcaa ttttggtcat ccggcgtaac ggacgggcgg gaaatcaacg ctttggcgag
    22561 ttctagggat tgggtttcgg tcatttttcg gcttttctgt ttgagatgcc gtctgaacgg
    22621 agtttcagac ggcatttgat gttgaatgga acgggctaac gatagcgaag cgggttcttc
    22681 aggtcaagca ccatgacacg cttcataaag cggcagcaaa tcttccaccg tttccgctat
    22741 ccatttcgcg acatcctgcc tgcccaaatc gtcgcgttcg atatgtttgc cgatgcagaa
    22801 gaagtcttcg ccgttttgca actttcggtc gggttcgctt tgttgcgcga cggttcggta
    22861 gtcgtcgtat tcgctttccg caccgtgcca catatcgaaa gaagcgtatt tttcggtatc
    22921 aaaattatcc aaccagcggt tgtaatcagg cagcgcgatg ggggaaacat cggctttata
    22981 gcagtgccaa tccaagctga cgctcagacg gcggcggttg agcagtatcg acaaaatcgc
    23041 tgcggaattt ttatattgtt cgtatttgaa gtaggcaaag aaatgggcgc gaacctgcca
    23101 gccgttgcac cagcgttcga tgtgcggcgg cgcaaacggc gcacccaatt cggcggcaac
    23161 ctgctgaatc agctgctgcc atatctgcca gttttccttg tagtcggctt tgatttgcgg
    23221 aatgctttca ggctggtatt ttttaagctg ggaaaattgg aaaaacggga tattgaacaa
    23281 atcgcaactt ttcggggtca gcataatata tccttgagac gattgtttca gacggcatta
    23341 tctgcgccgg cgcgccgcca tgatttcgcc gatttcggtc agtttctctt tggggatgaa
    23401 catcttgccc atgtcgaaca agggttcttc aatcgccaaa tgcacatcat atcccgccac
    23461 aaagcgtttg aacgcttccg catcgggaat ataggcattg tctgcttcga gtttggcaaa
    23521 ttcggcagcc acggcatccc agttgccatg caggcttaca tgttggcgta aaagctcgtc
    23581 cacgctttct tgggcttgcg gcgcatattg cagcaggagc gggaaaaagt tttcttcttc
    23641 gtcctcatgg tgcagcggcg cggcaacgtt gaaataccgg gtgatttggc ggatggtttg
    23701 caaaacaagc tgattgcagc cgttttcggc gatgtagtcc gacagcatcg cgacttggcc
    23761 gcaaaaacgg cgtactttgc cgtggcaggc atacagcatt tcaatcggtt cggcaaaggt
    23821 aacgcttttg gtttcaaacg gattcatgtt ttcgttctca acgggcactt ttcaagcagt
    23881 cattttataa taaaacagcc tgcacaaagc aggctgtccg tcttttgaga ctttaagcgg
    23941 attaatcgac caaagtcact ttgccgctca tcaaagcacc gtgacctggg aaggtacaag
    24001 cgaatttata ttcgccgtcg gccaatttag caggatccag agtcagggaa gcttcttcgc
    24061 cgccgccgat cagtttggta tgggcaacaa cgcgtgcatc atcaggtttg acatagtcgg
    24121 tatcggcagc acctacgccg tctttaaata cgccgtccat gtcttcagct ttggcaatca
    24181 cgagattgtg acccatgctg gctttgggtt gcgtaccggt atgtttcaga gtgatggtga
    24241 actctttaca tgctttgctg acttggatgt ctttggtgtt gaactgcata ttgtcgttgg
    24301 attcgacagt tgccgcacag ttgccggcag caggggcttc ggcagcatct gcaggagcag
    24361 cttcggcggc aggtgcttcg gaagcgggtg cttcagcagc aggagttgcc tcggcagcag
    24421 gcgcggcagg ttcttgagag caggcagcca aaccgataac ggcggcagaa atcagagcca
    24481 gatacgcttt cataacaaat ctccaatcga taaaataata ttcggtttta cagaaatcaa
    24541 agtgcaaccg ccattaacaa aaccttgaaa aagattccgc cgcgttgcac aaacagatgt
    24601 ttcggagcgg cattttgcta caaatttcat ttgaaatcaa agcctgtctg caagtttaca
    24661 atcgtttact ttaaaagggg caattttacc ccgaacctat ttctttagta ttagacccat
    24721 tatcctttac ttcttaatat taacggatgt ttacacaaat tcccgtatac attttatgcg
    24781 ccatgccttc taaccaagtt tgccaatgcc tccgccaatt cgggatgccg tttttccaac
    24841 tttaccgccg ccgaaccgaa actctccagc gcagccttac tcaaatgcag ggtattggtt
    24901 ttcggcggtt tttccggttt cgggaccagc ctgaccgaaa cagagcgtat cgaagcgtca
    24961 agccctgcca actgcggcaa taccgacggt gcaatcattt tcaagcgcga tgccgccata
    25021 ttgtttgccg ccaaaaggac aagcctgccg tcttcgatac ataccgtctg aaaatgcggg
    25081 tgcaggttgg caggcagcag ttttttcacg gcggcatcca accgccgcca ctgtcccgcc
    25141 tgtttcaaaa gtccggaaag cagcgcgtcc cgcctgccca actgttccaa attcataaaa
    25201 catacaccca aaaagattga aataccgcaa acgcgccttt atttcagacg gcattagcac
    25261 tttgcacaaa cgcttgtgtt aaaatcgcgt tttcgcccac tattatatca ggcgcaggaa
    25321 ttattcatgc tgacaaacat tgccaagaaa atcttcggca gccgcaacga ccgcttgctg
    25381 aaacaatacc gtaaatccgt tgccagaatc aacgcgctcg aagaacagat gcaagcccta
    25441 agcgatgctg atctgcaagc caaaactgcc gaattcaaac aacgcctcgc cgacggtcag
    25501 actttggacg gcattttgcc cgaagccttc gccgtctgcc gcgaagcgtc ccgccgcacc
    25561 ctcggtatgc gccacttcga cgtgcagctt atcggcggta tggtgctgca cgacggcaaa
    25621 atcgccgaaa tgcgtaccgg cgaaggcaaa accctggtcg ccaccctcgc cgtctatctc
    25681 aacgcgctgg ccggcaaagg cgtacacgtc gttaccgtca acgactacct cgcctcacgc
    25741 gatgcgggca ttatggagcc gctctacaat ttcctcggcc ttaccgtggg cgtgattatt
    25801 tcagatatgc agccgttcga ccgtcaaaac gcctatgccg ccgatatcac ctacggcacc
    25861 aataatgaat tcggcttcga ctacctgcgc gacaatatgg ttaccgacca atacgacaaa
    25921 gtgcagcgcg aattgaattt tgctgttgtc gatgaagtgg attccatctt gattgacgaa
    25981 gcgcgcactc cgctgattat ctccggtcag gcggatgaca acatccagtt gtaccaaatc
    26041 atgaacaccg ttccgcccca cctcgtccgt caagaaaccg aagaaggcga aggcgactat
    26101 tgggtcgacg aaaaggcaca tcaggtcatc ctgagcgaag caggtcacga acacgccgag
    26161 caaatcctga cccaaatggg attgctggca gaaaacgact ccctctattc cgccgccaat
    26221 atcgccctga tgcaccacct tatggcggca ttgcgcgcgc attccctctt ccacaaagac
    26281 caacattacg tcatccaaga cggcgaaatc gtcatcgtgg acgaattcac cggccggctg
    26341 atgtccggcc gccgctggtc ggagggtctg catcaagccg tcgaagccaa agaaggcgtg
    26401 gaaatcaaac gcgaaaacca aacccttgcc tccatcacct tccaaaacta tttccgcctg
    26461 tacaccaagc tctccggcat gaccggcaca gccgataccg aagctttcga gttccaaagc
    26521 atctacaacc tcgaaaccgt catcatcccg accaaccgcc ctgtacagcg caaagacttc
    26581 aacgaccaga ttttccgttc cgccgaagaa aaattcgaag ccgtcgtcaa agacatcgaa
    26641 gaatgccaca aacgcgggca gcccgtcctc gtcggtacga ccagcatcga aaactccgaa
    26701 ctggtctccc accttctgca aaaagccgga ctgccacaca acgtcctcaa cgccaaagaa
    26761 cacgaacgcg aagcgctgat tgtcgcccag gcaggcaaag tcggcgcgat taccgttgcc
    26821 accaatatgg cgggacgcgg tacggacatc gttttaggcg gcaacctgaa gcaccaaacc
    26881 gatgccatcc gtgccgacga agccttgagc gacgaagaga aacaggcaca aatcgccgca
    26941 ctcgaaaacg gctggcaggc agaacacgat aaagtaatgg aagcaggcgg cctgcacatc
    27001 atcggtacgg aacgccacga aagccgccgt atcgacaacc aattgcgcgg acgttccggc
    27061 cgtcagggcg accccggatc cagccgtttc tacctttcgt ttgaagaccc gctgctgcgc
    27121 ttattcgcac tcgaccgcgc cgccgccatc ctcaaccgtc tcgcccccga acgcggcgtt
    27181 gccatcgaac acaacctgct gacgcgccaa atcgaagggg cgcaacgcaa agtcgaaggc
    27241 agaaacttcg atatgcgcaa acaggttttg gaatacgacg acgttgccaa cgagcaacgt
    27301 aaagtcattt acagccagcg caacgaaatt ctgaccagca aagacatcag cgacctgatg
    27361 aaggaaatcc gttctgatgt cgtcagcgac ctcgtggata cctatatgcc gcccgacagc
    27421 atggaagaac aatgggacat cccgactttg gagaaccgtc tggctgccga attcagactg
    27481 cacgaagaca tccaatcctg gctgaaggcg gacaatgcga ttgacggtca agacatcaaa
    27541 gaacgcctga tcgaacgcat cgaaaacgaa tatgccgcca aaaccgaact ggtcggtaag
    27601 caggcaatgg ccgatttcga gcgcaacgtg atgttgcagg tcatcgacaa ccaatggcgc
    27661 gaacacctcg ccgctatgga ctacctgcga caaggcatac acctgcgcag ctatgcccaa
    27721 aaaaatccga agcaggaata taaacgtgaa gcctttacca tgttccaaga cctgtggaac
    27781 ggcatcaaat tccatattgc ctccctgctt acctcggttc aaatcgaaca aaaccctgtc
    27841 gcggtggttg aagagcaacc catcggcaac atccagtcca tccattccga atcgcccgat
    27901 atggaagaac ttttgggtca gtcgcaaacc gatttggtta ccgaggcctt taatcccgat
    27961 ggaacggatt tcagccccga agccttggaa gcgcggggac aaatcgtcca ccgcaacgac
    28021 ccctgcccct gcggcagcgg tttgaaatac aaacaatgcc acggcaaact ggcttaagcg
    28081 tttgaacgca aatgccgtct gaacatccga tttccggttt cagacggcat tttgcctgaa
    28141 ccgccacatc cgaccgccat tccgaaaaat cccgatttcg taccgtccaa actaaaaaca
    28201 ggcatccgcc ccgccgaact atgattccat ccgacttcat tgacgaactt ctggccaaaa
    28261 ccgatattgt cgacatcatc gacgaacagg ttccgctgaa aaaaggcggg gcgaactata
    28321 tggcgtgttg cccgttccac aaggaaaaaa cgccgtcgtt ttcggtcagt ccaaccaagc
    28381 agttttacca ttgtttcagt tgcggggcgc atggttcggc gattggtttt gtgatggagc
    28441 atcaggggct gtcgtttccg gaggctgtgc agtttcttgc cgaccgtgtg ggcatggttg
    28501 tgcctaaagt gcgtgggcag aacgataatc ccgaagtccg tgccgaacgt aagaaaaaac
    28561 agcagacgct ggaagaaacg acggcggcgg cggctgattt ttacgcgcaa cagctaaaat
    28621 tcaatccggc ggcaaaagct tatttggaca agcgcggctt gagtgcagaa gttatcgcgc
    28681 attatggttt gggctacgcg cccgacggct ggcagccttt ggcgcaagtg ttccaaccgt
    28741 atccgaatac cgcgttggtg gatacgggga tggtgattga caatgagggg cggtattacg
    28801 accgcttccg ccatcggatt atgttcccta tccgcaatcc gcgcgggcag gtcatcggtt
    28861 tcggcggcag ggtgctggac gactccaaac ccaaatatct aaactctccc gatacgcctt
    28921 tgtttgataa ggggaaaaac ctttacggat tgtatgaagg gcgtgctgct gtcaaggaag
    28981 tggggcggat tctggtggtc gaaggctata tggacgtggt cgcgctggca cagttcggcg
    29041 taggctacgg tgtggcggcc ttgggcacgg caacaacagc ggaacacgtc aaaatcctga
    29101 tgcgtcaggc ggacagtatt tatttctgtt tcgacggcga cagcgcgggg cgcaaagcgg
    29161 cttggcgcgc gctggaaaac gcgctgccgc aattgaagga cgacaaatcg ctgcattttt
    29221 tgttcctgcc cgaggaacac gaccccgaca gctacatccg cgcctacggc aaagcgcaat
    29281 ttgaagatgc gctgttgaat caaagcaagc ccttgtcgga atatttttgg gaacatctta
    29341 cagacggcat tcatctcaat acgcaggaag gcaaggcgga attggtaaaa accagttcgc
    29401 cgcttttggc gcagattacc gcgccggcat tggcttattt gttaaaacaa cggcttagcg
    29461 agctggtcgg catcgacccc gacaacctcg cgcaactgct cggacaggaa gcgccaaagc
    29521 ggcacgtcaa acaaaaaaac tacaaactgc ccccgatttc cgtcaaacag cccgttatgc
    29581 cgacattggt gcaacggcaa atccgcagcc tcttgataaa tccggattgg gctgcatata
    29641 tagacctgcc cgattatttg gcgttggacg gcgatttcgc ctgccttgcc aacctcgccg
    29701 aaaccattaa aaaccatcct tccgtgccgg caaccgcaca ggttttggaa catatgcgcg
    29761 gttcgcccta cgaagaaaca atcaaccgca tcttccggtc ggcccttcaa tcggaagaaa
    29821 tggaaggcgg cggcgaagaa gattgcgaaa acttccaaat cggcatcaaa aaactgctca
    29881 atgagttaaa atacagccaa atcgaaacat taaaacaaaa aagcctgcaa tccggcttaa
    29941 atgaaagcga gaaaaaactt ttgctgtcgc tgctgaccgc aaaacaaaat tgaccggcgg
    30001 attccgccat ccgtaaaccg ttatgccgtc tgaaaagcat tcaccccggc tgcaacaacg
    30061 acacctgcag aacacccatc ccaaaaagcc ttcagacggc atcagagtac cctactctgc
    30121 cacgccttca ggtgcgtcca aacgcaaacc gtcggcatct tgccaacaga aagcagacaa
    30181 tgtccagaaa ccaaaatcac gaagaatacc aagacgatgc ccgccccctg acgattgagg
    30241 aacaacgcgc tcgcctgcgc caactcatca ttatgggcaa agaacgcggc tacatcacct
    30301 actccgaaat caacgacgca ctgcccgacg atatgtccga tgccgatcaa atcgacaaca
    30361 tcgtcagtat gatttccggt ttgggtatcc aagttaccga acacgccccc gatgcggaag
    30421 acatattgtt aagcgacaat gccgccgtta ccgacgatga tgccgtcgaa gaagccgagg
    30481 ccgccctttc cagcgcagat tccgagttcg gcaggacgac cgaccccgtc cgtatgtata
    30541 tgcgcgaaat gggacaggtc gacctgctga cccgcgaaga cgaaatcatc atcgccaaaa
    30601 aaatcgaaaa cgccctgaaa aatatggttc aggccatctc cgcctgcccg ggatccattg
    30661 ctgaaatctt agaactcatc gaaaaaatcc gcaaagacga aatccgcgtc gacgaagtcg
    30721 tagaagccat tatcgacccg aatgaagtat tgctcaacga attgggcttg gggcacttgg
    30781 aaaccacagc acccgagaaa ccttccaacg acaattcgga cgaaaacgaa gacgacgaag
    30841 aatcggaaga agatgcggat gaaatctcgg cagccaatct cgccgaattg aaacaaaaag
    30901 tcatcggcca ctttgcccaa atcgaaaaag actacaaaaa aatgattggc cgtttggaaa
    30961 aacaccacag ccggcacaaa gactatctcg cctaccgcga cgcgattgcc aacaaacttt
    31021 tggaagtccg cttcgccacc cggcaaatcg acagcctcag cagcagcctg cgcggaaaag
    31081 tagaaaacat ccgcaaactc gaacgcgaaa tccgcgacat ctgcctcgac cgcgtccata
    31141 tggagcgcga ctacttcatc caaaacttcc tgcccgaaat caccaatctg gaatggattg
    31201 aagaagaaat cgccaaaggc agggtttgga gcgacgcgct cgaccgcttc cgccacgcca
    31261 tcctcgaaaa acaaaccgag ttggcggata tggaaaaaga aacccgcatt tccatcgaag
    31321 agttgaaaga aatcaacaaa aatatggtgt caagcgaaaa agaaaccgca gctgccaaac
    31381 aggaaatgat tcaggcaaac ttgcgcctcg tcatttccat tgccaaaaaa tacaccaacc
    31441 gtggcttgca gttccttgat ttgattcagg aaggcaacat cggcctgatg aaagcggtcg
    31501 acaagttcga ataccgacgc ggctataagt tctccaccta cgcaacctgg tggatccgtc
    31561 aggcaatcac ccgctcgatt gccgatcaag cgcgtaccat ccgcattccg gtacatatga
    31621 ttgaaaccat caacaagatg aaccgcatct cgcgccaaca ccttcaagaa accggcgaag
    31681 aacccgattc cgccaaactt gccgaactga tgcagatgcc cgaagacaaa atccgcaaaa
    31741 tcatgaaaat cgccaaagag ccgatttcga tggaaacccc catcggcgac gacgacgatt
    31801 cgcacttggg cgacttcatc gaagatgcca acaatgttgc gccggccgat gcggcaatgt
    31861 acaccagcct gcacgaagta accaaagaaa tactcgaaag cctgaccccg cgtgaggcaa
    31921 aagtcctgcg tatgcgtttc ggcatcgata tgaacaccga ccacacgctg gaagaagtcg
    31981 gcagacagtt tgacgtaacg cgcgaacgca tccgacaaat cgaggcaaaa gcactccgca
    32041 agctgcggca tccgacaaga agcgaccgtt tgagaagttt cttggacagc gaagacagca
    32101 agctgtaaac caaaaaaccg caggtttcaa atacctgcgg ttttttctta cacaataaac
    32161 aacgcttcca catatcccac actcctatcc cgagaccttt gcaaaattcc ccaaaatccc
    32221 ctaaattccc accaagccat ttaggggatt ttccatgagc accttcttcc ggcaaaccgc
    32281 acaagccatg attgccaaac acatcgac
//
LOCUS       DAXIEB010000009        31939 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000009 DAXIEB010000000
VERSION     DAXIEB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.009"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..176)
                     /locus_tag="ACFOA3_000362"
     CDS             complement(<1..176)
                     /locus_tag="ACFOA3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6897423.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCKPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIP"
     gene            complement(564..839)
                     /locus_tag="ACFOA3_000363"
     CDS             complement(564..839)
                     /locus_tag="ACFOA3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6897424.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(836..2119)
                     /locus_tag="ACFOA3_000364"
     CDS             complement(836..2119)
                     /locus_tag="ACFOA3_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6897425.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWTEVFVITSLFMLPGLLMTLFLAREPVLPLAVSKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGMWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTSERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTRDAGR"
     gene            complement(2123..2248)
                     /locus_tag="ACFOA3_000365"
     CDS             complement(2123..2248)
                     /locus_tag="ACFOA3_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897426.1"
                     /translation="MPSEQSFRRHFAFERQNGGAGAVFFSGWAIFATIGFYFLAV"
     gene            complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="ACFOA3_000366"
     CDS             complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="ACFOA3_000366"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6897427.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3712..3921
                     /locus_tag="ACFOA3_000367"
     CDS             3712..3921
                     /locus_tag="ACFOA3_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897428.1"
                     /translation="MAKYAERGGLTQSRHDFTTPMPSETGFASNFKPENIVSDGKIVN
                     NMLRFVCSKAECPPFCPNADKTDAV"
     gene            3999..4808
                     /gene="aroE"
                     /locus_tag="ACFOA3_000368"
     CDS             3999..4808
                     /gene="aroE"
                     /locus_tag="ACFOA3_000368"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6897429.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHRQFALQEGVEIEYGRICADIG
                     SFAQAVLTFFGTGGCGANVTVPFKQEAFDLADEHSERASAAGAVNTLILLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLLAQNPARIVIANRTRAKA
                     EEVAARFGIEAVSPDSLAGGFDIIVNGTSGGLSGQIPDIPPDIFQNCALAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFKPDIRPVIEYMKAL"
     gene            4810..5511
                     /gene="mtgA"
                     /locus_tag="ACFOA3_000369"
     CDS             4810..5511
                     /gene="mtgA"
                     /locus_tag="ACFOA3_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6897430.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVPLDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPEDDTD"
     gene            complement(5713..5788)
                     /locus_tag="ACFOA3_000370"
     tRNA            complement(5713..5788)
                     /locus_tag="ACFOA3_000370"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5753..5755),aa:Phe,seq:gaa)
     gene            complement(5979..6713)
                     /gene="lptB"
                     /locus_tag="ACFOA3_000371"
     CDS             complement(5979..6713)
                     /gene="lptB"
                     /locus_tag="ACFOA3_000371"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6897431.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGENFKY"
     gene            complement(6787..7317)
                     /gene="lptA"
                     /locus_tag="ACFOA3_000372"
     CDS             complement(6787..7317)
                     /gene="lptA"
                     /locus_tag="ACFOA3_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6897432.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQGDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(7298..7879)
                     /gene="lptC"
                     /locus_tag="ACFOA3_000373"
     CDS             complement(7298..7879)
                     /gene="lptC"
                     /locus_tag="ACFOA3_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6897433.1"
                     /translation="MKIRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVKTEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGLLNFSSKVKATIYDTKDM"
     gene            complement(7876..8412)
                     /locus_tag="ACFOA3_000374"
     CDS             complement(7876..8412)
                     /locus_tag="ACFOA3_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6897434.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPCGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8585..9559)
                     /locus_tag="ACFOA3_000375"
     CDS             complement(8585..9559)
                     /locus_tag="ACFOA3_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6897435.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9655..10710
                     /gene="tal"
                     /locus_tag="ACFOA3_000376"
     CDS             9655..10710
                     /gene="tal"
                     /locus_tag="ACFOA3_000376"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6897436.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALEILVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKTAYQDWEQYFTAPKFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVDEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            10852..11142
                     /locus_tag="ACFOA3_000377"
     CDS             10852..11142
                     /locus_tag="ACFOA3_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897437.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            11159..12046
                     /gene="gluQRS"
                     /locus_tag="ACFOA3_000378"
     CDS             11159..12046
                     /gene="gluQRS"
                     /locus_tag="ACFOA3_000378"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6897438.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(12114..13124)
                     /gene="dusA"
                     /locus_tag="ACFOA3_000379"
     CDS             complement(12114..13124)
                     /gene="dusA"
                     /locus_tag="ACFOA3_000379"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6897439.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDAMLLKDNDGGLILEAWEEVERANT
                     RE"
     gene            complement(13155..13646)
                     /gene="dtd"
                     /locus_tag="ACFOA3_000380"
     CDS             complement(13155..13646)
                     /gene="dtd"
                     /locus_tag="ACFOA3_000380"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6897440.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13720..14439)
                     /locus_tag="ACFOA3_000381"
     CDS             complement(13720..14439)
                     /locus_tag="ACFOA3_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6897441.1"
                     /translation="MLAGFAAAKAPEIDPALVDTLVAQIMQQADRHAEQSQKPDGQAI
                     RNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEYVRFLERSETVSES
                     ALRQFYERQIRMIKLQQVSFATEEEARQEQQLLLKGLSFEGLMKRYPNDEQAFDGFIM
                     AQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDAQPFELVRNQLEQG
                     LRQEKARLKIDAILEENGVKP"
     gene            complement(14548..15414)
                     /locus_tag="ACFOA3_000382"
     CDS             complement(14548..15414)
                     /locus_tag="ACFOA3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6897442.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15463..15741)
                     /locus_tag="ACFOA3_000383"
     CDS             complement(15463..15741)
                     /locus_tag="ACFOA3_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6897443.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15741..16031)
                     /locus_tag="ACFOA3_000384"
     CDS             complement(15741..16031)
                     /locus_tag="ACFOA3_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6897444.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16034..16564)
                     /locus_tag="ACFOA3_000385"
     CDS             complement(16034..16564)
                     /locus_tag="ACFOA3_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6897445.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16872..19547
                     /gene="tspA"
                     /locus_tag="ACFOA3_000386"
     CDS             16872..19547
                     /gene="tspA"
                     /locus_tag="ACFOA3_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6897446.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKLQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENVTETVAETSDFHTAADDLSALLQPFETPAVE
                     KFEAPAIEEKAAETVDKTPDFHTVADDLSALLQPAEVPAVEENAAEITLETPDSNTSE
                     ADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKYDLAEMYLE
                     IGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19720..20136)
                     /gene="gloA"
                     /locus_tag="ACFOA3_000387"
     CDS             complement(19720..20136)
                     /gene="gloA"
                     /locus_tag="ACFOA3_000387"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6897447.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20160..21329)
                     /gene="lapB"
                     /locus_tag="ACFOA3_000388"
     CDS             complement(20160..21329)
                     /gene="lapB"
                     /locus_tag="ACFOA3_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6897448.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21340..21654)
                     /locus_tag="ACFOA3_000389"
     CDS             complement(21340..21654)
                     /locus_tag="ACFOA3_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6897449.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            21964..22962
                     /gene="ilvE"
                     /locus_tag="ACFOA3_000390"
     CDS             21964..22962
                     /gene="ilvE"
                     /locus_tag="ACFOA3_000390"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6897450.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23206..23991)
                     /gene="fabI"
                     /locus_tag="ACFOA3_000391"
     CDS             complement(23206..23991)
                     /gene="fabI"
                     /locus_tag="ACFOA3_000391"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6897451.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24146..24967)
                     /gene="dapD"
                     /locus_tag="ACFOA3_000392"
     CDS             complement(24146..24967)
                     /gene="dapD"
                     /locus_tag="ACFOA3_000392"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6897452.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25134..26777)
                     /gene="pgi"
                     /locus_tag="ACFOA3_000393"
     CDS             complement(25134..26777)
                     /gene="pgi"
                     /locus_tag="ACFOA3_000393"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6897453.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETGCHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27134..28366
                     /gene="pilG"
                     /locus_tag="ACFOA3_000394"
     CDS             27134..28366
                     /gene="pilG"
                     /locus_tag="ACFOA3_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6897454.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28440..29300
                     /locus_tag="ACFOA3_000395"
     CDS             28440..29300
                     /locus_tag="ACFOA3_000395"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6897455.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29302..29934
                     /gene="coaE"
                     /locus_tag="ACFOA3_000396"
     CDS             29302..29934
                     /gene="coaE"
                     /locus_tag="ACFOA3_000396"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6897456.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            29927..30136
                     /locus_tag="ACFOA3_000397"
     CDS             29927..30136
                     /locus_tag="ACFOA3_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6897457.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(30221..31897)
                     /gene="pilB"
                     /locus_tag="ACFOA3_000398"
     CDS             complement(30221..31897)
                     /gene="pilB"
                     /locus_tag="ACFOA3_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6897458.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7497 a         8984 c         8182 g         7276 t
ORIGIN      
        1 gggataacca ttttggtgcc ctgaaaatca aatgtaaccg tatgttcaaa tcctgtcatt
       61 ggctcgggat tgttgaaact ggtttgttca ttaacaggtt tacatgaggt catagtaaaa
      121 acactcccaa taaacaaaat aaaaagttga taaatagtag tagcatatgg tctcataatt
      181 tcaagcctag aaattagtta aagaatatat tcatggtaga tactaactct ttaagttgac
      241 atattgtgcg atatcgaggg ctgtaccata ttttcccatc gggtcttccc cgttctttat
      301 atgagcggca ggcatcctca taaaagaagg gataccgata aggaaaaata cctcggcata
      361 aaaaatatac tgattaatca atacaaatcc aacaaacagt ttatcatttt cttttctgtt
      421 ggcgtataaa aaatactgat atatgccttc actttcatag ggcgacgctt tatgtaccgt
      481 cctgtgtgtt gaaacatcgg ttttccttac cgcctgccct gcttttccct gattgctgcc
      541 aactgccgtc cgaacttgaa ccgtcaggtt tcagacggca ttttttctgc ctgttcccac
      601 aatttccgtt tttgtgcggc ggtcagtttt ttgacggcga tttcttgttt gagcgcggtg
      661 cctttatcca tcccgcctgc aacgatacgc atcgctaccg gtttgaatac gcgggtatat
      721 ttcgcgcctt ttccggctgt gtgggcggca agccgctgtt gcggattcgg gctgatgccg
      781 caatagaacg cgctgttttc acacaatatc aggtaaacgc tccagtttga cgcgttcatc
      841 tgcctgcatc ccgagttttc tccccgttcc aaggcgcaac tttcagcaac aacagcatac
      901 ccggcagggc aagtatgaaa cacagccgga aaaacggtac ataaccgagc cattcgatca
      961 gataaccggc aaaggaattg atgaccgtac ggggaacagc ggacagactg gtaaacagcg
     1021 cgagctgcgt tgctgtaaac gcgggattgg tctcgcgcgc catatacgat acgaacgccg
     1081 ccgtccccaa ccccacgccg actgcctctg cgccgataac tgccgccagc atcagcctct
     1141 cgcttgtacc gactgtgtcg aaatgtccga acccggccag ccatacaaac cccaaaaccg
     1201 ttattgcctg caccgcgccg aatagccaca aggctttgtt cacgccgatt ttcagcatcc
     1261 acatgccgcc caagatacct gccgccactg ccggccacag tcctgcattt ttggcaatca
     1321 aaccgatgtc ggtcttgctg aaacccatat ccagataaaa cggcgttgcc aacgcggttg
     1381 ccatactgtc gccgagtttg taaaggaaga taaacagcag cacgcataca gccgaagcga
     1441 tgcccttgcg tgtaaaaaat tctttaaacg gctctaccac ggtctgtttc aacgttttag
     1501 aaacagcaag cggcaacacg ggttcgcgcg cgagaaatag cgtcatcaga agaccgggca
     1561 gcataaacag cgaagtgatg acaaacacct ccgtccacgg catcctgtct gccaacacca
     1621 aactcaatga accgggaatc agggcggcaa cccgataggc gttcacatga accgagttgc
     1681 ccaaacccaa ttcttcgtcc gacaaaatct cgcgcctgaa cgcatccaat acaatatcct
     1741 gactggcgga aaaaaaagca acaagcaccg acaagccagc aatcaacggc agatgattac
     1801 gggggtttaa aaaggcatat gccgccaaag ccgccagcaa ccctgcctgc gtcaacagca
     1861 tccacccgcg ccgccgcccc aaaacgggca gcctgaccgc gtccatcagc ggggaccaca
     1921 aaaatttcca agtaaacggc agaccgatta acgccatcag cccgatgctc ttcaaatcca
     1981 cctgctcgct gcgtaaccat gccggaatca ggttaatcag aaagtacagc ggcagccccg
     2041 aggtaaaccc cgtaaaaata cagacaagca tatttcggga aaagatttgc ccgataaaac
     2101 ctgatttcga tacagtcatg gttcaaacag ccaagaaata aaagcctatt gtagcaaata
     2161 tcgcccaacc gctgaaaaac accgcccctg ctccgccgtt ttgccgttca aatgcaaaat
     2221 gccgtctgaa actttgttca gacggcattt ctactgaaac ctgagagttt acaggctgta
     2281 atacatttca aattccagcg gatgcggtgc catgcggatg cggcggacat cttcctcttt
     2341 aaaggcgatg tagctgtcga tccagtcttt gctgaacacg ccgccgcgca ggaggaattc
     2401 gtggtcggct ttgagggcgg ccagtgcttc ttccaaagag gcgcaaacgg tagggacgag
     2461 cgcgtcttct tccggcggca ggtcgtagag gtttttatcg gcagggtcgc ccggatggat
     2521 tttgttttga ataccgtcca aaccggccat caacaaagcg gcgaacgcca agtatgggtt
     2581 ggcggtcggg tcggggaaac gcgcttcgat gcggcgcgct ttgctgctgt tcacagacgg
     2641 aatacggatg gacgcggaac ggtttttggc ggagtaggcc agtttggtcg gcgcttcgaa
     2701 gtgcggcacg aggcgtttgt aggagttggt ggacggattg gtaatcgcgt tcagggcttt
     2761 ggcgtgtttg atgatgccgc cgatgtagta gagcgcggtg tcgctcaagc cggcatagcc
     2821 gtcgcctgcg aacaggtttt gaccgtcttt ccaaatggat tggtgaacgt gcataccgct
     2881 gccgttgtcg cccataatcg gtttgggcat aaaggtggct gttttgccga agttgtgggc
     2941 aacgttttga atcacatatt tcatgtcttg ggtttggtcg gcgcgtttga ccaaggtggc
     3001 gaaacgcgtg ccgatttcca tttggctgcc ggtaccgact tcactgtggt ggacttcgac
     3061 ttcgatgccg agttcttcca aaatgtttac catcgcggag cgcaggtctt ggccgcaatc
     3121 aatcggcgcg acgggggcgt aaccgccttt gacggcaggg cggtggccgg tgttttgacc
     3181 gtccatgtgc agaccgcttg cccatgcgcc gctttcggac gtgatttcgt aacgggtttt
     3241 gtgcatgtcg gtttcaaatt ctacgccgtc gaagacgaag aattcaggct cggggccgaa
     3301 gtatgccgtg tcgccgatgc cggaagattt caaataggcc tcggcgcggc gtgcgatgga
     3361 gcgcgggtcg cggtcgtaac cctgaccgtc ggcagggtcg atgacgtcgc aggtaatgac
     3421 gacggtaaca tcatcataaa aaggatcgac gaaggctgta gacgcatcgg gacgcagctg
     3481 catatcggaa gcctgaatgc ctttccagcc gccgatagac gaaccgtcaa acgcctgacc
     3541 gttttcgaac cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg
     3601 ctgcttgcct ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat
     3661 caattttacg gcgtttttaa tggacatttt tcagctcctg gaaaaaggga aatggcaaaa
     3721 tatgcggaac gcggcggctt gacgcaaagt cggcacgatt ttaccacgcc gatgccgtct
     3781 gaaaccggtt ttgcgtcaaa tttcaagccg gaaaatatag tttcagacgg caagattgtc
     3841 aacaatatgc ttcggtttgt ctgttccaaa gcggaatgcc cgcccttttg tccgaatgcc
     3901 gacaaaacag atgccgtctg aaagcagttc agacggcatc gttcccatct tcgcgccgcc
     3961 gttataaaat gaccgccccg ccttgatgga atatgactat gcacgccctc ccccgctacg
     4021 ccgttttcgg caaccccgtt gcacacagta aatcgccgca aatccatcgg cagtttgccc
     4081 ttcaggaagg cgttgaaatc gaatacggac gcatttgcgc cgacatcggc agttttgcac
     4141 aggcggtttt gacatttttt ggaacaggcg gttgcggggc aaacgtaacc gtcccgttca
     4201 agcaggaagc gtttgacttg gcggacgaac attccgaacg cgcatcggcg gcaggcgcgg
     4261 tcaatacgct gattttgctg aaaaacggaa aactgcgcgg cgacaatacc gacggtttgg
     4321 gcttggtcgg cgatatagtg aaggtgcaac acacagaaat tgaaggcaaa aatatcctat
     4381 tgctcggcgc gggcggcgcg gtgcgcggcg tgattcccgt attgctggcg caaaatcccg
     4441 cccgcatcgt catcgccaac cggacgcgtg ccaaagcgga agaagttgca gcgcgtttcg
     4501 gcatcgaagc cgtctctcct gacagtttgg caggcggttt cgacatcatc gtcaacggca
     4561 catccggcgg tttgagcggt cagattcccg atattccgcc cgatattttt caaaactgcg
     4621 cgcttgccta cgatatggtg tacggcgaag cggcaaaacc gtttttggat tttgcccggc
     4681 aatcgggcgc gaaacaaacc gccgacggac tgggtatgct ggtcgggcag gcggcggctt
     4741 cctacgccct ctggcgcggt ttcaaaccgg acatccgccc cgtcatcgaa tatatgaaag
     4801 ccctgtaata tgttccgcat cgtcaaatgg ctgattgccc tgcccgtcgg catctttatc
     4861 tttttcaatg cctatgtgta cggcaacatc atcacctacc gcgccgtcgc gccgcatcgg
     4921 actgccttta tgtcgatgcg gatgaagcag tttgaacaag aagggcgcga tgttcctctg
     4981 gattaccgct ggatgcccta caaccgcatt tcggtcaacc tgaaaaaagc cctgattgct
     5041 tccgaagatg cccgtttcgc cggacacggc ggcttcgatt ggggcggcat tcaaaacgcc
     5101 atcaggcgca accggaacag cggcgaagtg aaggcgggcg gatcgaccat cagccagcag
     5161 cttgccaaaa acctgttttt gaacgaaagc cgcagctata tccgcaaagg cgaagaagcc
     5221 gcgattactg cgatgatgga agccgttacc gacaaagaca ggatttttga actgtattta
     5281 aactcaatcg aatggcacta cggcgtgttt ggtgcggaag ccgcgtcccg gtatttttat
     5341 caaatacccg ccgccaaact gagcaaacag caggcggcga aactgactgc ccgcgtcccc
     5401 gccccgctct actacgccga ccatccgaaa agcaaacggc ttcgcaacaa aaccaatatc
     5461 gtgctcaaac gtatgggttc ggcagaattg cctgaagacg acacggactg agtgccgccg
     5521 cgatataaaa tgccgtctga aatatcattc agacggcata tgctttctgc ctttaccctc
     5581 ttctccaaaa cgcaacgggg cggtcattat catttttgcc cttctacata acggtcagac
     5641 acaaaacgtg tccgacacgg acaatttccc aaaaacggca ggcaaaaaaa taaccgcttc
     5701 aaagcggttt tttggtggcc agaggcggaa tcgaaccgcc gacacacgga ttttcaatcc
     5761 gttgctctac caactgagct atctggcctt tgtctgtctc attaagagat gcgtattaaa
     5821 ccaaattacg ggacaacacg caagcggatt tgtcgatatt ttttacccaa ccgactaagt
     5881 cgatgtttta agtttaaatt aatttcacag aaaaatagtg caaatcggtt ttttgccgtt
     5941 tgttcggcga tgttctgctt ttcagacggc aaaacgcttc aatatttgaa gttctcaccc
     6001 agataaacag aacgaacctg ttcgtttccg accaaatcat caggttttcc cgatgccagc
     6061 accgtgccgt ctgaaataat gtaggcgcgg tcgcagatgc tgagggtttc gcgtacgttg
     6121 tggtcggtaa tcagtacgcc gatgccgcgc gatttgagaa atccgatgat tttttggatg
     6181 tcgatgacgg cgatgggatc gacgccggca aaaggctcgt ccaacaaaat aaaatgcggt
     6241 ttcatggcaa gtacgcgggc gatttcgacg cgtcggcgtt cgccgcccga caaagatggg
     6301 gcaggattat tgcgcaaacg ctcgatattc aggtcggcaa gcagtttttc aacttcgccg
     6361 tcgatgcggc ttttgtcttt ggtactgatt tctaaaatgg cgcggatgtt ttgttcgacg
     6421 gtcattttac ggaaaataga ggcttcctgc ggtaggtagc cgacacccag gcgggcgcgt
     6481 tcgtgtatgg gcaggtgacg caattcctgc ccgtccagca tcacgctgcc cgcatcggcg
     6541 gcaatgagtc caacaatcat gtagaagctg gtggttttac ccgcaccgtt gggcccgagc
     6601 aggccgacga cttccccgct ttcgatttca agggagaagc ttttaacgac ttggcgtttt
     6661 ttaaaactct tttgcaggtt ttgaacaaca aggcggctga cgtttgcgct cataaatatc
     6721 tcttcaatat cttcggtcgg catatgccgt cggaaccggg tttatgtttc agacggcatt
     6781 ttgggattat tcggattttt gcgtactcga aggctggatg atgacgctga ccctgccgga
     6841 tttggaagcg gattttgcgc cggattttgt actgccgctg atggtataga cttcggtttt
     6901 ggtgttgtag gtaatcaccg cgccttcggc gacatcgccg ccgcgctgta ctttggcatt
     6961 accggttaag actacggtgc tgcctgcaga cgaataagca acgttgtttg cctgaccgcg
     7021 caccgtccct ttgcccccgt ccaacgtttg gctgaagcgt acgggcgagc cgtcggcctt
     7081 cataaactgt tcgcctttgt cattacgcgt tacgttgacg cgggcggcgg aaatattgag
     7141 cgtaccctgt ctgatgacaa cgtttccgct aaatgtagtg ctttggttgg cttgattgag
     7201 agaaccttgg tcggcctcaa tctgaatagg ctgcctgctg tcgccttgaa gggcaaaagc
     7261 gggagatgcc gaaaaaaatg ctattaaaac aaatagctta catatctttt gtatcataaa
     7321 tcgtggcttt cactttagat gagaagttca acaggcctgt tttgtggtcg taagtcatgc
     7381 cgcccgcctg cccgtgcgac gcaccgtatt gaaagctgac gggcgtatcg gtttgggcat
     7441 attgagattc ggtatcgacg tgcagctttt cggttttaac tttacccgcc tgccgtttgc
     7501 catcggcggt tttggtcagc acaacgttgt ttttaaaaag aacctgtttg ttttcggtat
     7561 ggtaaacggc ttcatcgctg ccgacttcgt acaacaacct gccttcttgg aagaacacga
     7621 gatgcggcga atcaaaatgg atgtcgctgc tttcgggaaa ctgtttcgcg cctttcgcgc
     7681 tcaaatgttc ttttaagtat ccctgttcgt caaaccgcct gccatccaat ccgtccattg
     7741 tgtattgcgg ttcgtcggga ttgagcctga cttcttcaat ctcgacttcg ctgatgcgcc
     7801 ccaaccatgc cgacaggctg cccaaggcaa ccgccaatat caatgggaac gcaattccgt
     7861 accgccatct tattttcatt tgatgtactc gttcaaagcc gcgcccaaag tcccttgcgc
     7921 ctgcataatc aggtcgcaca cttcgcgcac cgcgcccgca ccgcaggggc gtttggcaat
     7981 ataggcggcg tgttgcagcg taaaccaatg cgcgtcgggg acggcaaccg gcaatccgca
     8041 acgcaccatc accggcaaat cgaccacgtc gtcgccgaca aaggcacact cggcttcttc
     8101 cacgccaacc tgagcgcgca gctcttcata cgcggcgcgt ttgtccgaaa tgcctttgaa
     8161 gtagtaattt atgcccaact gtttgacgcg gatgccgacg gagggcgcgt cccgacctgt
     8221 gataatcgca gtctgcacgc cgcttgcctg aagcattttc agaccgtgtc cgtccagtgt
     8281 gtgaaacgat ttgatttctt cgccgttatc gcggataaag atgcgcccgt cggtcaaaac
     8341 gccgtccaca tccaatatca gcagtttgat tttggcggcg cgcgcctgta attcgggaga
     8401 aattgcctgc atgggatttc cttgttttca gacggcacat tttagcacaa atgccgtctg
     8461 aaacggctgc aacgccttat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     8521 cagacagtac agatagtacg gcaaggcaag gcgaggcaac gctgtactgg tttttgttaa
     8581 tccactatac aatccgtgcc gccagcaggt cgtgcatatt cagcgcgcca atcagtacgc
     8641 cgtcggcatc agtaaccaga agcccattca catggtttgc ctgcatgact ttcagggctt
     8701 cggtggcgag gcgttcggcg gagatggttt taggatgcgt atgcatgact tcgtctatcg
     8761 aaagaccggt aaaattgtcg cattcttgaa acaggcggcg caaatcgccg tcggtgaata
     8821 cgcctttcag acggccttgc ccgtccgtta ccgccagcat ccccagccct ttttcactca
     8881 tgctgacgat ggcttctttc aagggcgtgc cgagtcggac ggcgggcagg ccgccgcctt
     8941 tgtgcataat gtcggcaacg cgcaaaagta ggcgtttgcc gaggctgccg gcaggatggc
     9001 tcaaggcgaa atcgtcgggc gtgaacgcgc gtgcgcgcag caggacgacc gccaacgcat
     9061 cgcccaaagc catgacggcg gtggtgctgg tggtcggggc aagccccagc gggcaggctt
     9121 ctttggaaac cgacgccgtg atgtggatgt cggcatggcg cgccatggtt gaatcggggc
     9181 gggcggtgat gcagacaagc gtgatgtctt tgcgtttgag cgcggggatg atggcggcga
     9241 tttcgtcgct ttcgccggaa ttggaaatcg cgacgaccac gtcgttgtcc acaatcatgc
     9301 ccaaatcgcc gtgtgccgct tccgcagggt ggacgaaaaa cgcgggcgtg ccggtcgagg
     9361 ccatggttgc cgccattttg cgcccgatat gtcccgactt gcccatgccc gtgataacaa
     9421 ccctgccctt gcagtgcaac aacgcgtctg ccgcaaggac gaagttttcg tccaattccg
     9481 ctgcaatttc gcgcaagcct tccgcttcgg tgtgcaacac ttcgcgcgcc cagtcgagat
     9541 attttccgtt ttctgccatt gccatccgcc tttgctgtaa caaaactacc caaaatttcc
     9601 gaatcggggt agcataagca ttcaaacccc gccatccatc caaaggaatc agaaatgact
     9661 attttatcgg acgttaaagc attaggacaa caaatctggc tggacaacct ttcccgctcg
     9721 ctcgtgcaaa gcggcgaatt ggcgcaaatg ctgaaacaag gcgtgtgcgg cgtaacttcc
     9781 aatcccgcca tcttccaaaa agccttcgcc ggcgacgcgc tttacgccga tgaaatcgcc
     9841 gccctcaagc agcaaaacct cagccccaaa caacgctacg aaaccatggc ggttgccgat
     9901 gtgcgtgccg cctgcggcgt ttgccttgcc gaacacgaat ccaccggcgg caaaaccggt
     9961 ttcgtcagcc tcgaagtttc gcccgaattg tccaaagacg cgcaaggcac ggtagaagaa
    10021 gcgcgccgcc tctacgccgc catcgggtgc aaaaacgcga tgattaaagt gcctgccacc
    10081 gacgcaggca tcgatgcgct cgaaatcctc gtttcagacg gcatcagcgt aaacctcacg
    10141 ctgctgttct cacgcgccca aaccctcaaa gcctacgccg cctacgcgcg cggcattgcc
    10201 aaacgcttgg cagccggaca aagcgttgcc catatccacg tcgtcgccag cttcttcatc
    10261 tcccgtgtgg acggcgcgct ggacacaacg ctgcccgacc acctcaaagg caaaatcgcc
    10321 atcgcccttg ccaaaaccgc ctatcaagat tgggaacaat atttcaccgc ccccaaattt
    10381 gccgcactgg aagcccaagg cgcaaaccgc gtgcagcttt tatgggcatc taccggcgtg
    10441 aaaaacccag cctatcccga cacgctctac gttgacagcc tgatcggcgc gcacaccgtc
    10501 aacaccgttc ccgatgccac gctcaaagcc tttatcgacc acggcacagc caaagccacg
    10561 ctgaccgaag gcgtggacga agcacgggcg cggctcgctg aaattgccgc gctcggcatc
    10621 gatgtcgaaa ccttggcggc gcgtttgcag gaagacggtt tgaaacagtt tgaagaagcg
    10681 tttgaaaaac tgctcgcacc gttggtttaa acttttcatc cccgatgccg tctgaaaggc
    10741 tttcagacgg catcgccctt ttgaccggac acagtgggtt caccctaaaa atattaatca
    10801 acaagaagtt aatgcacgac attacccacc aacacaaaag gaaatagaat catggcaaaa
    10861 gcactcgaaa tcatttcacc caatgaaatt tattcagatc taatttttaa ggatccggta
    10921 cctccccata ctatatatac agaattgatg aaactgattg gtagaaatgc aagtaatgag
    10981 agaatcgcag atagagcctt cgattttttc tcgtccggcc ttatggatgg ttcagctacc
    11041 gaagagcaat ataatgctct atatgattta accttgcttg aagagcccgg gatggaactg
    11101 aataaagatg agataatggc tttgattcat agtcttaaat aaaggggctg taccggatat
    11161 gtacacagga cgtttcgccc ccagcccgac cgggctgctc cacatcggct cgctgctgac
    11221 cgccgccgct tcctatgccg atgcacgctc aaacggcggc aaatggctgg tccgcatgga
    11281 agacctcgat ccgccgcgcg aaatgccggg ggcggcaagc catatcctgc acacgcttga
    11341 ggcattcgga ttcgagtggg acggagaagt cgcctatcag agccaccgtt acgccctgta
    11401 tgaagaaacc ctatgccgtc tgcaaaccgc cggactggtc tatccctgcc attgcagccg
    11461 caaagactgg caggccgggg caaggcgggg cgcagacgga ttcgtctata acggacgttg
    11521 ccgccacccc ggccaacgcc ctgcaccgca aggcaaacag ccggcgtggc gcatccgcgt
    11581 ccccgaccgc attatcggtt tttcagacgg catcgtcggc ggttacgccc aaaacctcgc
    11641 ccgcgacatc ggcgatttcg tcctgcttcg tgcagacggt tactgggcat accaactcgc
    11701 cgtcgttgcc gacgatgccg aacagggcgt tacccacatc gtccgcggac aagacctgct
    11761 cgtttccact ccgcgccaaa tctatttgca gcagtgtttg ggcgttccga caccgcaata
    11821 tgcccacctg ccgctgctga ccaacgcaca agggcagaaa tggtcgaaac agacgctcgc
    11881 ccccgcattg gatttaaacc gccgcgaaca actcctccgc caagtgttcc gttacctcaa
    11941 gctgcccgaa gcaccggaaa ccgaccgccc tgccgaactg ctcgactggg cggtggcaca
    12001 ctgggatatg gacaaagtgc cgaaacacgc cattaccacc ccctaaacac cgcccgacaa
    12061 acgcacaaat gccgtctgaa actttcagac ggcatttcac atacagcccc gccctattcg
    12121 cgcgtatttg cccgttcgac ttcttcccac gcttcgagaa tcaggccgcc gtcgttgtct
    12181 ttcaagagca tcgcgtcgga aagcatacgc cgccaagtcc gcgcaccttt caaaccgtgc
    12241 atcagcccga ggctgtggcg gacgatgtgg cgcaagattg tgccgcgtcc ggcttggatc
    12301 tgggcttggc tgtatgtgta gagacgctgc accaaatcgg cgtattcaat cgggctgcgg
    12361 gtatcgccgt aaaacagcct gtcccattcg cgcatcacca tcgggttgtg gtacgcctcg
    12421 cgcccgacca tcacgccgtc aacgtgttgc aggtgtccgg cgattgcttc gttggtggtg
    12481 atgccgccgt tgatgatgat ttccagcccg ggaaactcct gcttgaggcg gtaaacgtaa
    12541 tcgtatttca acgggggaac gtcgcggttt tctttggggg aaagaccgtc cagccaagcg
    12601 ttgcgggcgt ggacgataaa ggttttgcag gcggttttgt cgcgcagcgt gccgacgaaa
    12661 tcggcaacgg tttggtattc ggtctgcctg tccacaccga tgcggtgttt gacggtaacg
    12721 ggaatcttga ccgcatcctg catggcgttg aggcagtcgg caaccagccc gacttcgttc
    12781 atcagacacg cgccgaacga gcctttctgc acgcgcggac tggggcagcc gcagttgagg
    12841 ttgacctcgt tgtaaccgta tgcctcggcg gctttggcgg ctttcgccaa atcggacgga
    12901 tcgctgccgc ccagttgcag ggcgacgggc tgctcgcctt cgttgaacat caaaaagcgg
    12961 tctttgtcgc cgtaaacaat cgcaccggca ttgaccattt cgctgtacag ccaagtattt
    13021 cgggtaatct ggcgggcaag gtaacggtag tgcctgtccg tccagtcgag catcggggcg
    13081 acggaaaggc ggcgcggggg aagggtttgc gtgttgtcgt tcattgtcga tatttcacca
    13141 ttgcggattc aatttcaatc cggaacaacc ttcatttttg gggaaatccg cgtcatgaaa
    13201 gagtccagca gtatggttac cggcccgtcg ttacagagcg acacctgcat atgcgtgcgg
    13261 aaacgccctg tttcgacatg aatcccgtgt ccgcgcaaca gttccgccgt tcgcaggtaa
    13321 agctgctgcg cctgttctgc aggtgcggct tgggaaaacg aaggccgccg cccgcttgcc
    13381 gcatcggcat aaagtgtaaa ctgcgacacc agcagcaccg cgccgccgac atctttcaaa
    13441 gacaggttca acttgcccgc ttcgtcttca aacacgcgca aatgggcgat tttgtcggcg
    13501 atatagcgtg catctttttc tgtgtcgcta tgcgttacac cgagtagcac gacgaatccg
    13561 ccgtcgattt tgccgcaggt ttccgtgccg ttttcggaca cgacatccac ctttgcacct
    13621 accgttttct gtatgaccgc acgcataagc cgtcctcctt acctgaacgt gcaatgccgt
    13681 ctgaaaaggc ttcagacggc atcggtattg gaaatgccat tacggtttga caccgttttc
    13741 ttccaaaatg gcatcgattt tcaagcgggc tttttcctgt ctcaaacctt gttccaactg
    13801 gtttctgacc aactcgaaag gctgcgcgtc ggggtttttc ccgacctcgc tgagtttgaa
    13861 cagataatag cgttcgccca atttgaccgg atcgcgggta acgtcgcccc gattcatcgc
    13921 tgcaaactgc gaagccagcg gctcgggaag ctgctgcgcc ataatgaaac cgtcaaacgc
    13981 ctgctcgtcg ttcggataac gcttcatcag cccttcaaaa gacagccctt tgagcaggag
    14041 ctgctgctcc tgacgcgcct cctcttcggt tgcgaagctg acctgctgca atttgatcat
    14101 gcggatttgc cgctcataaa actgacgcag tgcgctttcg gaaaccgttt ccgaacgttc
    14161 cagaaaacgg acgtactcct cggcataaaa agacgcttcg gcgattttaa agcggttttg
    14221 gacatcctta tccttatcca aaccttcctt caatgccctg tttttcaaaa cttccaaagt
    14281 ttgcagccga cggacggcat cgtttcggat tgcctgcccg tccggttttt gggactgctc
    14341 cgcatgccgg tctgcctgct gcatgatctg cgccaccagc gtatccacca aagccgggtc
    14401 gatttcgggt gctttggctg ccgcaaaacc tgccaacatt gcagcaataa ctgcggcagc
    14461 ggttttttct gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc tgtctgaacg
    14521 tcttgccgcc atatcccgtt ttcagaatta ttttgcaggt ttgatgtttg ccttgcccaa
    14581 cagtgcaccg acggcacggt caatccgttc cgcctgaagg ttgcccgcaa tctgtccttt
    14641 catttcatca aaagaaggca cttttacctc gcggctgtcg ttgacataat aaacgccgta
    14701 gaaatcgccg tttttcagcg gcgttgccgt aaattcgcct tttttcaagt ccttaattgc
    14761 ctgataaagc ggcggaacac cctgttccaa atctttcagc ggcacatatc cgaccggcgc
    14821 accggtctgt ttggtacggt cgttgaggga atattgtttc aagacggcat cgaaaccttt
    14881 tttcgccttc aagtcggcaa ccgctttttt tgcattttct tccttgtcgg tcaggatttc
    14941 gcccaactgg acttcctgcg tacctttgta aaaaccgctg atattgtcat atgcggcttt
    15001 tacttcctgc tcggaaaccg gttgggtttt ggcgatatgc aatgcgtatg cctcgccgtt
    15061 caagccatat tttaccgcct gccaaacggt tttgaaggac ggtttcttgt cgtcgcccga
    15121 ctttttcgct tcggcacgca atttggcaag cgcattttta aactctgccg accggtcgag
    15181 tttcaggcgt ttcacttcct gtgcgaccac ggtattgacc acttcgtttt ccagcaggga
    15241 ttggcgcagt tgcggcgtgt cttcggcacg gctgttttcc gcacggaatg cggcaacctg
    15301 cgcatcgatg acggaactgt cgattttctg accgttgacg gttgccagcg tttgggcaaa
    15361 caggctgccg gaacaggcaa gcagtgcaac ggaagtcagg atttttgctt tcataatctt
    15421 ctctctttaa attttaaatt gaaatgttcg ggcagacttc attcagtccg ccgtatggaa
    15481 atactcgtca ggggtagccg cccggatgcc gagcgcgtga atcatgccgc ctgaaaacaa
    15541 atctttgagc agcgatttaa ccgttttctg gcggttcagg cggcttacgc cttcaaaacg
    15601 gccgctaacg accaaaacgg cataatgtcc gccgccggta ttgcccgcgt gtcctttgtg
    15661 cagatggctc tcatcgccga tttccaacac cagcggatcg agcgtctgca ggcgttcgcg
    15721 gatcaaatcg acggccggca ttatttgaac accgctttaa acggcttgat cagcacttcg
    15781 ctgtacacgc ctgcatgaac atagggatcg tcttccgccc acgcctctgc cgcatccaaa
    15841 gactcgaact gcgccacaat caaactgccc gaaacgcggt tggagtcctc cggcaaagga
    15901 ttcgggcctg ccgtcaacag ccggccttcc gacttcagcg tctccagccg tttgaggtgt
    15961 tcgggacgtg ccgccatacg cgcctcgtgc acatcctccc cgtctgttgc cagcaacata
    16021 aaatattcca cagtcaatcc tcctttttca gacaggtact cagataaata ccctgaataa
    16081 taaagaaaac aagcatcagt gcagtcgagc cgaacatttt atagttgacc cattgcgact
    16141 cgaaccgggt aaacacaaac cagttggcga tacccataaa aatcaggaaa ccgacccaca
    16201 tatacgtcaa tttcgcccat acggcatccg gaagctgaat ctccctgccg atactcgcct
    16261 tcaagccgtt tttaccggcg aggtggctgc cccacaggaa cagcgcgccc agccaaaaca
    16321 aaacgctcgg cttccacata atgaagcggc tgtcgcccaa aacaatggtt gcgccgccga
    16381 ataccacaat cagcaccaat ccgacccact gcatcgtatc cagcttttta tatttccaat
    16441 acagaaaagc cgcctgaacc acaccggcaa ccaaggcaac cgccgtagcg gcaatcatat
    16501 ttttggtaac ggtataagta gcaaaaaaca agatgacaga caaaaggtcg ctgacgaatt
    16561 tcatatccaa atgagaaagc aaatcaaata aacgctaatc atacaggcag aaccctgttt
    16621 ttgcacctgc aaaaccaaca aattttgttt tgattgatgc aaatatgcac agatgttttt
    16681 gaaaaaagat ggagatatgt cataatttgt aaaaacggcg aatgattgcc gtttaaaatg
    16741 tggcggcggt cggtacattc acatattaaa tgcccgccgg ccgactgccc cgccgatgcc
    16801 cgatgatgac gaaatttaca gactgtacgc ggtcaaaccg tattcagccg ccaacccaca
    16861 ggggatacat cttgaaaaac aacagacaaa tcaaactgat tgccgcctcc gtcgcagttg
    16921 ccgcatcctt tcaggcacat gctggactgg gcggactgaa tatccagtcc aaccttgacg
    16981 aacccttttc cggcagcatt accgtaaccg gcgaagaagc caaagccctg ctaggcggcg
    17041 gcagcgttac cgtttccgaa aaaggcctga ccgccaaagt ccacaagttg ggcgacaaag
    17101 ccgtcattgc cgtttcttcc gaacaggcag tccgcgatcc cgtcttggtg ttccgcatcg
    17161 gcgcaggcgc acaggtacgc gaatacaccg ccatcctcga tcctgtcggc tactcgccca
    17221 aaaccaaatc tgcactttca gacggcaaga cacaccgcaa aaccgctccg acagcagagt
    17281 cccaagaaaa tcaaaacgcc aaagccctcc gcaaaaccga taaaaaagac agcgcgaacg
    17341 cagccgtcaa accggcatac aacggcaaaa cccataccgt ccgcaaaggc gaaacggtca
    17401 aacagattgc cgccgccatc cgcccgaaac acctgacgct cgaacaggtt gccgatgcgc
    17461 tgctgaaggc aaacccaaat gtttccgcac acggcagact gcgtgcgggc agcgtgcttc
    17521 acattccgaa tctgaacagg atcaaagcgg aacaacccaa actgcaaacg gcgaaaccca
    17581 aagccgaaac cgcatccatg ccgtccgaac cgtccaaaca ggcaacggta gagaaaccgg
    17641 ttgaaaaacc tgaagcaaaa gttgccgcgc ccgaagcaaa agcggaaaaa ccggccgttc
    17701 gacccgaacc tgtacccgct gcaaatactg ccgcatcgga aaccgctgcc gaatccgccc
    17761 cccaagaagc cgccgcttct gccatcgaca cgccgaccga cgaaaccggt aacgccgttt
    17821 ccgaacctgt cgaacaggtt tctgccgaag aagaaaccga aagcggactg tttgacggtc
    17881 tgttcggcgg ttcgtacacc ttgctgcttg ccggcggagg cgcggcatta atcgccctgc
    17941 tgctgctttt gcgccttgcc caatccaaac gcgcgcgccg taccgaagaa tccgtccctg
    18001 aggaagagcc tgaccttgac gacgcagcag acgacggcat agaaatcacc tttgccgaag
    18061 tcgaaactcc ggcaacgccc gaacccgctc cgaaaaacga tgtaaacgac acacttgcct
    18121 tagatgggga atctgaagaa gagttatcgg caaaacaaac gttcgatgtc gaaaccgata
    18181 cgccttccaa ccgcatcgac ttggatttcg acagcctggc agccgcgcaa aacggcattt
    18241 tatccggcgc acttacgcag gatgaagaaa cccaaaaacg cgcggatgcc gattggaacg
    18301 ccatcgaatc cacagacagc gtgtacgagc ccgagacctt caacccgtac aaccctgtcg
    18361 aaatcgtcat cgacacgccc gaaccggaat ctgtcgccca aactgccgaa aacaaaccgg
    18421 aaaccgtcga taccgatttc tccgacaacc tgccctcaaa caaccatatc ggcacagaag
    18481 aaacagcttc cgcaaaacct gcctcaccct ccggactggc aggcttcctg aaggcttcct
    18541 cgcccgaaac catcttggaa aaaacagttg ccgaagtcca aacaccggaa gagttgcacg
    18601 atttcctgaa agtgtacgaa accgatgccg tcgcggaaac tgcgcctgaa acgcccgatt
    18661 tcaacgccgc cgcagacgat ttgtccgcat tgcttcaacc tgccgaagca ccgtccgttg
    18721 aggaaaatat aacggaaacc gttgccgaaa cacccgactt caacgccacc gcagacgatt
    18781 tgtccgcatt acttcaacct tctgaagtac ctgccgttga ggaaaatgca gcggaaaccg
    18841 ttgccgatga tttgtccgca ctgttgcaac ctgctgaagc accggccgtt gaggaaaatg
    18901 taacgaaaac cgttgccgaa atacctgatt tcaacgccac cgcagacgat ttgtccgcat
    18961 tacttcaacc ttctgaagta cctgccgttg aggaaaatgt aacggaaact gttgccgaaa
    19021 cgtccgactt ccacaccgcc gcagacgatt tgtccgcgct gctgcaacct tttgaaacac
    19081 ctgccgttga gaaatttgaa gcaccggcta ttgaagaaaa agcagcggaa accgttgata
    19141 aaacgcccga cttccacacc gttgccgacg atttgtccgc gctgctgcaa cctgctgaag
    19201 tacctgccgt tgaggaaaat gcagcggaaa tcactttgga aacgcctgat tccaacacct
    19261 ctgaggcaga cgctttgccc gacttcctga aagacggcga ggaggaaacg gtagattgga
    19321 gcatctacct ctcggaagaa aatatcccaa ataatgcaga taccggtttc ccttcggaat
    19381 ctgtaggttc tgacgcgcct tccgaagcga aatacgacct tgccgaaatg tatctcgaaa
    19441 tcggcgaccg cgatgccgct gccgagacag tgcagaaatt gctggaagaa gcggaaggcg
    19501 acgtactcaa acgtgcccaa gcattggcgc aggaattggg tatttgattc ccaactgccc
    19561 tttcgcagat caaggatgcc gtttcagacg gcatcttttt ttgccttatc ggtgtaacgg
    19621 ataaagtttg aaccggcaca ggctcaaaca gcaggtcgac ggcaacaaaa tgccgtctga
    19681 aacccctaaa ggcttcagac ggcattggcg gcggcggtat caggcagttt gataggcaac
    19741 cgaatcgtcg ccgcttttct tttgaatgaa ctcgattttg tatccgtcgg ggtcttcgac
    19801 gaaggctatc acggttgtgc cgtgtttcat cgggccggct tcgcggacga cgtttccgcc
    19861 ctgccgcttc acacgttcgc aggcttcgta ggcatcgtcc acttcaaccg cgatgtgtcc
    19921 gtaggcgttg cccaagtcgt atcgttccgt atcccagttg tgcgtcagtt ccaaaaccgt
    19981 gctgtcggtt tcatcgccgt aaccgacgaa ggcaagggta aatctgcctt cgggataatc
    20041 ttttcggcgg agcagtttca tacccaaaac gttttggtag aaatcgaggg atttttcgag
    20101 attgcccacg cggagcatag tatggagtaa gcgcattttt gtgttcctct cggtagtggt
    20161 taaacttcga ttttgtttgg cgtaaacgtc tgccatttgt tgcaggcagg acaatgccag
    20221 aaaaagactt gtgatttgaa gtggcagttt cggcaccggt acatcacgct gcgctgtagc
    20281 tgccgtccga taaccgaacg catcatgtcg gcatcggctt tccaagccgg attcatatcg
    20341 ctgagtttca aaccgagcag gcggtacacg ccgttaaggt cgggcttgcg gcggacaagc
    20401 tcgacggcgg tttgcgcggc ttctttctcg cccttaagca gcagggattt ctcgtacacg
    20461 acattgatca ggtcaagttc gggaaacgtc tgcatatatc ctgtcagacg gttcaagcct
    20521 tcttcaggtt ttccctgcgc ggcataggct tcgtaaagct tctcgccgac catgctcaag
    20581 tatgcatggt tttgctgctc gatggcggca taggcttcga cggcggcagg gaaattgcct
    20641 tgtcggtgtt cgatgtcgcc caaaatcatg ttggcgcggg tgcatttttt gttggcttcg
    20701 agtgccttgc cgacattgaa acgcgcggca tcgaaattgg acttgaacag cgcggcttgg
    20761 gcaagttcgc aatagaactg tgcaatctca aactgatagg tctgctcgtc atggctgagc
    20821 agccgggcgg tttcaaccgc tttttcccaa tccctgtcct gttggtagat attgagcagg
    20881 tgctgtctgg cttcacgcgc catttcaccg tcttgcagcc ccaaaaaaat ctgttcggca
    20941 cggtcaacca accccgcact ttgatagttt tgcgccaatt caaataatac gcgcgcacgc
    21001 ttggctccgg ttgtatcggg agagtcaagc aatgtttggt gcatattgat ggctttgtcg
    21061 ttttcgccac gctgacggta aagcttgcct agggtgaggt tcaggtcgta tgactgaggc
    21121 tgctgatcga tgacctctgc caattccctg gcggcacgtc cgctgttgcg gtcaaccaag
    21181 gcatccagac ttttataaaa tcccgacggt atgctttttg cctgctttaa tacagtcttc
    21241 atatccacgc gggcggcaaa ccagcccatc gcgaagaaaa cgggcaaaag gataatcggc
    21301 agcaggataa tccacaattc attgtccata tcggctttct caaggctgtt tggcagattc
    21361 gggcgcattt tgcgccggtg gtgcggtcag ctccttcccc gtcaaacgcg catttttctt
    21421 tacttcggca cgcaacctgc cgttctcgcc acgtaacgac aacaaccgtc cgaacaaggc
    21481 aaacattcca aaaacaatac cgactacaaa tgcgccgaac aatacgacaa tcagcggcaa
    21541 atcgaatttt tgccccggca ggtaggaaaa ggtaacggca tccgtattaa tgacagcaag
    21601 cagcaggaag agcagcagga taatgatttt gatgacggta tagataagtt tcatgtttgc
    21661 tccgtaaata aaaacgccgc ccggacagct tcgcactctg aaccaagatt gcgctagttt
    21721 aaacgatttg cacggtttcg gataggatgc ggcagcgtgt tcggacgaca tacggagtat
    21781 gcgattgccg acaattttac caaatacacc gttcctttcc atttgaaaaa taacggattg
    21841 gacaccgcat cgacagaaaa acccgccgcg cacttgtcaa aaccctgttg gcaggcgtat
    21901 ctttacaatc ttcaaattca aaccgttcat tgaaacatat cagaataaga aaggctttac
    21961 atcatgagca gacccgtacc cgccgtattc ggcagcgttt ttcacagtca aatgcccgtc
    22021 ctcgcctacc gcgaaggcaa atggcagccg accgaatggc aatcttcgca agacctcacc
    22081 ctcgcacccg gcgcgcacgc cctgcactac ggcagcgaat gctttgaagg cttgaaagcc
    22141 ttccgtcagg cagacggcaa aatcgtgctg ttccgtccga ctgccaacat cgcgcgtatg
    22201 cggcaaagtg cggacatttt gcacctgccc cgccccgaaa cccaagccta tctcgatgct
    22261 ttggtcgaac tggtcaaacg cgccgccgat gaaattcccg atgcgcctgc cgccctgtac
    22321 ctgcgtccga ccttaatcgg taccgatccc gttatcggca aagccggttc tccttccgaa
    22381 accgccctgc tgtatatttt ggcttccccc gtcggcgact atttcaaagt cggctcgccc
    22441 gttaaaatct tggtggaaac cgaacacatc cgctgcgccc cgcacatggg ccgcgtaaaa
    22501 tgcggcggca actacgcttc cgccatgcac tgggtgctga aggcgaaagc cgaatatggc
    22561 gcaaatcaag tcctgttctg cccgaacggc gacgtgcagg aaaccggcgc gtccaacttt
    22621 atcctgatta acggcgatga aatcattacc aaaccgctga ccgacgaatt tttgcacggc
    22681 gtaacccgcg attccgtact gacggttgcc aaagatttgg gctataccgt cagcgaacgc
    22741 aatttcacgg ttgacgaact caaagctgcg gtggaaaacg gtgcggaggc gattctgacc
    22801 ggtacggcag ccgtcatctc gcccgttact tccttcgtca tcggcggcaa agaaatcgaa
    22861 gtgaaaagcc aagaacgcgg ctatgccatc cgtaaggcga ttaccgacat ccagtatggt
    22921 ttggcggaag acaaatacgg ctggctggtt gaagtgtgct gatgctttaa ataaaaaatg
    22981 ccgtctgaaa cccgtttggc gtttcagacg gcattttcgc atccgaaccg tttccgctgc
    23041 acctgcagca agtcggcaca aaggcaatcg gttaaaacaa gcgtccgcat ttcccatccc
    23101 gcctgccgta agtcgggcat ttctctagaa atacgcttca gacggcaaac cgccgcccga
    23161 aaccgatatg cggtacggac ggcgcacgga tttgaaaacg gcggattatc cctctgtgct
    23221 caaggcattg atgctgtaac cgccgtcaac gtaagtgatt tcgccggtaa tgccggacga
    23281 caggtcggac agcaggaagg cggcggtatt gccgacttct tcgatggtaa cgttgcggcg
    23341 gagcggattg tgggcggcga cgtgtcccaa gagtttgccg aaatcggcga tgccggaggc
    23401 ggcaagcgtt ttgatcgggc ctgcggaaat gccgttgcag cggatgcctt ctttgcccag
    23461 acaggcggcg gtaaaacgga tgcccgcctc aaggctggct tttgccatac ccatcacgtt
    23521 gtaattcgga atcgcgcgca ccgcgcccaa gtagctcagg gcaacgatgg cggagtttct
    23581 gccgcgcatc atcggacggg cggcttttgc caatgcgggc aggctgtatg cggaaatttc
    23641 gtgtgcggtg ttgaacgctt cgcggctgat gctgtcgagg aagtcgccgc tcaaggcttc
    23701 tttcggcgca aaaccgatgg aatgcaccaa accgtccaag ccgtcccaat gtttgcccaa
    23761 atcggcgaac acttggttga tttcgtcgtc gctggcgaca tcgcagcgga acacgagttc
    23821 ggaatccaat tccgccgcca ttttgcggac gcgctcttcc agtttgtcca caacgtaggt
    23881 aaacgccagt tccgcgcctt gttcgcggca ggctttggcg atgccgtaag cgatggaacg
    23941 ctcggaaatc atgccggtaa tcagaatttt tttgccttgc agaaaaccca ttttgtatcc
    24001 ttcaaatagt gttcggttcg ttctaaaccg ttgattatag caaattgtcc ctgtttctgt
    24061 gttttcacgt tgcagcatgc aaacggcaat gccgtccgaa gcggatttca gacggcatcg
    24121 gacgtttcaa atacggttta aggcatcaga tgccgcgcaa caattcgttc acgctggttt
    24181 tcgcacgggt ttgcgcgtcc acgcgtttga cgatgacggc gcagtaaagg ctgtggctgc
    24241 cgtctttgga aggcatactg ccggacacga cgaccgaacc tgtcggtacg cggccttggt
    24301 agatttcgcc ggtggtgcgg tcgaagattt tggtggattg accgatgaac acgcccatcg
    24361 aaatcacgct gccttcttcg acaatcacgc cctcaacgat ttcagaacgc gcaccgatga
    24421 agcagttgtc ttcaataatg gtcgggctgg cttgcagggg ttcgagtacg ccgccgatgc
    24481 cgacaccgcc gctcaagtgg acgtttttgc cgatttgcgc gcaagagccg acggtcgccc
    24541 aagtatcgac catcgcacct tcgtcaacgt atgcgccgat gttgacgtaa gacggcatca
    24601 gcacgacatt tttcgccaca aagctgccgc gtcgggcaac cgcacccgga acggcgcgga
    24661 agcctgcgtt tttgaactcg tcttcagacc agtcggcaaa cttggtcggc actttgtcga
    24721 agtatttgtt cacaccgtcg ttgaggattt cgttgtcttg gatgcggaag gacagcaaca
    24781 cggctttttt cgcccattcg ttaactttcc attcgcccac gcccaagcgt tcggcgacgc
    24841 gcagtttgcc ggaatcgagt tggcggatgg tttccaacac ggcttcttta acttcgggag
    24901 taacggtggt cggattaatg tccgcgcggt tttcaaaggc ggtttcgata atgttttgca
    24961 aagacataat gtttccttat gtgagatgtt tcgtgggacg cttgccggta ttttcaagca
    25021 gtatgttcat ttgaaatgaa tgcgctattg tgccacgcaa acgcatattc gtcagcaata
    25081 cgcgggcggt tgccgaacat tggcgtttcg gaatcgggcg ggcggcagta cctttatgcc
    25141 gcaccgccgc ctttcagacg gcattcgcgg taaaacgcca tcagcccttc ggtcgaggca
    25201 tcgtgtacgg acgtgccgcc ttccagttcg ccgatgatgg ttttcgccaa ctgtttgccg
    25261 tattccaccc cccactgatc gaaggggttg acgttccata tcgcgccttg gacgaaggtt
    25321 ttgtgttcgt aagccgccat cagcataccc aaattgtagg gcgtgaggcg gtcgagcagg
    25381 atgctgttgc tggggcggtt gccagggaac tctttgtgcg gcgcaaggcg ttcgcgttcc
    25441 gcttcgggca aatctgccag ttcggcgcgt gcttcgtcca aggttttgcc cttcatcagg
    25501 gcttccgctt gggcaaaggc gttggcaacg gtaaaacggc tgcgtccgtc ctccacgccc
    25561 tgcgccgtca ccgggacgat aaagtcgcag ggaatcaggc gcgtgccttg atgaagcagt
    25621 tggaaatagg cgtgctggca gttgacccct tcaccgccga acacgatgcc gcccgttttg
    25681 cacacggcgg gactgccgtc tgaagcgcgg cttttgccca aactctccat atcgagttgg
    25741 ttcagccacg ccggcagcag gcgcaggttg tggctgtacg gaacggcggt ctgcccgtcc
    25801 gcgtgttgga aattgttata ccacacggca atcagtgcca tcagaacggg aatgttgcgg
    25861 cgcggcggcg tgtggaaaaa atggctgtcc atcgcgtgcg cccccgccaa caactcgcgg
    25921 aaacgcgccc cgccgaccgc aaccatcacg ggcaaaccga cgggcgacca gacggaatag
    25981 cgtccgccca cccagtcgta catcgcaaac acgcgttccg ccgcaatgcc gaaactttgc
    26041 gccgcttccg tatcggcaga taccgcgcaa aaatgacatc ccgtttccga ttccgagaac
    26101 cccgcaccac gataccaagc cttgaccgcc tctgcattga gcagggtttc aggcgttttg
    26161 aaggacttgc tggcaacgca aaacactgtc gtttcggggt tcagacggca taaaaccgca
    26221 tccaggcagg caggatcggc gttggcggca aaatggacgg cgatatgccg tctgaacggc
    26281 tcaagtgcct gcacgcacat tgccggcccg aggtcggatc cgcctatgcc gatgtggaca
    26341 aaatccgtaa tccgtttccc agttatcccc tgatacgaac cgtcgtccaa actgtgtgca
    26401 aactccaacg cacgatttaa ctcgcggcgg atttcgggca acacgtccct gccgtccaca
    26461 taaacggcat ccgcaccgtc gggcaggcgc aaagccgtat gcagcgcggc acgcccctcg
    26521 ctgccgttga ctttcgcacc cgtccgcaaa gcacgcattt tcccttccaa atccgccgtc
    26581 tccgcaagac ggcagagcag ttgcagcgta tcttcgccca aacggttttt gctgtaatcg
    26641 aacaacatcc cgtccaaacg ctcatgcata cgctcaaaac ggtcgggttc gcaggcaaag
    26701 cggtcgcgca aaaggacatg acgcgtatcc tgataatggc gttcgagcgc ataccatgca
    26761 cgggtaaaag cattcatctg ttttccttga tttttcagaa ccggattaaa atgtagcaga
    26821 atgtagttta acaaacggca gcggctttgg cgaatctcca gaacaccgcc cgccgcgtcc
    26881 aatagcacct tatttccata tggcggaaat gccctccggc cggcatttga cccgcaacaa
    26941 tatcctgcaa gatttattgt gtacgcataa atgccggaca gccgcctaaa taaggtataa
    27001 tcgcatccga ttctgtcccg tttgccgccc gcaggcggga tgtcggtggt tttcatctta
    27061 ccgggaacac gccggtactg ttttttaacg atgtttctta cattttattc caattacttt
    27121 acggggctag aatatggcta aaaacggagg attttctttg ttcgcaaaga aagaaaaacg
    27181 ctttatcttt gaaggcaggc attccgcctc cgacaaactg gtcaacggcg aagtatccgc
    27241 gtttaccgaa gaagaggcgc gcaaaaaact ggcaaaacgc ggcatccgcc cgttgcagat
    27301 tactcgtgtg aaaacaagct ccaagcgcaa aatcacacaa gaagacatca ccgttttcac
    27361 ccgccagctg tccacgatga ttaaagcggg cctgccgctg atgcaggcat ttgaaatcgt
    27421 ggcgcgcgga cacggcaacc cgtctatgac ggaaatgctg atggaaatcc gaggcgaagt
    27481 ggaacagggc agctcgttga gccgcgcatt ctcaaaccac ccgaaatatt tcgaccgctt
    27541 ctactgcaac ctggttgcgg caggcgaaac gggcggcgta ttggaaagcc tgctggacaa
    27601 attggcaatt tacaaagaaa aaacccaggc tatccgcaaa aaggtaaaaa ccgcactgac
    27661 ctatccggta tccgtcatcg ccgtcgccat cggtttggta ttcgtgatga tgattttcgt
    27721 actgcccgcc tttaaagaag tttacgccaa tatgggcgcg gagcttcccg cactgaccca
    27781 aacagtgatg gatatgtccg actttttcgt ctcatacggc tggatggtgc tgatcgcact
    27841 gggctttgcc atatacggct tccttaaatt gaaggcgcgt tcgattaaaa tccaacggcg
    27901 tatggatgcc atactgctgc gtatgccgat tttcggagac attgtccgca aagggacgat
    27961 tgcccgctgg ggcaggacga cagcgacgct gattgcggca ggcgtgcctt tggtcgatgt
    28021 attggactcc actgccggcg cggcgggcaa tttaatctat gaagaagcca cccgggaaat
    28081 ccgtacgcgc gtcatccaag gtttgtctat gacttcgggg atgcgtgcga cggaactgtt
    28141 ccccaatatg atgttgcaga tgtcttccat cggcgaggaa tcgggttctt tggacgatat
    28201 gctcaacaaa gccgccgaat tttacgaaga cgaggtggac aatgcggtcg gcaggctgtc
    28261 cgctatgatg gagccgatca ttatcgtgat tttaggcttg gtcatcggca cgcttctggt
    28321 cgccatgtat ctgccgctgt ttaatttggg caacgtggtc gcctgatttg ccgcacagat
    28381 ccggcgcgga ttcgctctgc gccggtttgt ttttgttttg aatacattga gaacaaaata
    28441 tgtctgattt gtctgtattg tcgccgtttg ccgtgccttt ggcagcggtg ttcgggctgc
    28501 tggtcggaag tttcttaaac gtcgtcattt accgcgtgcc ggtcatgatg gaacgcggct
    28561 ggacggtatt tgccaaagaa tatttaaacc tgccgctgac cgaagaggaa agccgtacct
    28621 tcaacctgat gaaaccggat tcctgctgtc ccaaatgccg cgtgccgata cgcgcgtggc
    28681 agaacatccc gattgtcagc tacctgctcc tgcgcggcaa atgcgcttcc tgccaaacca
    28741 aaatcagcat acgttatccc ttaatcgagc tgctgaccgg cgtattgttc gggctggttg
    28801 cctggcaata cggctggtct tggattacat tgggcggttt gatactgacc gcgtttctga
    28861 tttccctgac ctttatcgat gcggacaccc aatacctgcc cgactcgatg acactgccct
    28921 tgatttggct ggggctgata tttaatttgg acggcggctt cgtgcctttg cagtctgccg
    28981 ttttaggtgc ggttgccggc tatggttcga tatggctttt atgcgcggta tataaactga
    29041 ttaaaggcga aatcggtatg ggcaacggag atttcaaact gattgccgca ttgggcgcgt
    29101 ggctcggcat atccgcattg cccgtgctga tttttgtttc ctcgctgatc ggtttggtcg
    29161 cggcaatcgt tatgcgcgtc gccaaggggc agcattttgc cttcggcccc gcactgacag
    29221 tttcgggctg gataattttt acggcaaacg attccgtatg gcgggcggtc aactggtggc
    29281 tgacccatcc ggtgctgtaa gatgacggta tgggtcgggc tgaccggcgg aatcggcagc
    29341 ggcaaatcgg cagccgcgca atgttttgcc gatttgggcg taccgcgtat cgatgcggat
    29401 gcggcggcgc actcgctgac ggcttcagac ggcatcgccc tgccggaaat caggcggctg
    29461 ttcggcgaca ccgttttcga cacacagggt ttgttgcggc gcgacatatt gcgtaaagaa
    29521 atctttgcct ccccatcgcg aaaagccttg ctcgaatccg tgatgttgcc gctgattttc
    29581 tcagaaatca aaaaacagca agaaaccttt accgatgcgg cttacggcat tgtcgaaatt
    29641 ccgctgctga cggaaaagcg tcaatttatc agcctgatac ggcgtgtcct gaccataagt
    29701 gcccctgtgg aaaaacgtat cggcagggtg atggcccgca gcgggctgac gcgcggcgag
    29761 gtggcggccg tcatcagcca tcaggcatcc gaatccgaac gcctgctgct tgcagacgat
    29821 gtgctgctca atgacggcag cctcaaaagc ctgcgtgaga aaacaatgcg cctgcacgcg
    29881 ttttattcag ggattttcgc ctcaaaacca acacaaggaa aacacaatga ctgaatcgcg
    29941 gcaaacacgc cttcaagtca aatgtccgac ctgtcaaaca gcagtagtat ggaaacccga
    30001 aaacgcattc cgccccttct gttcgcaacg ctgcaaactg atcgacttgg gcggatgggc
    30061 agacgggaaa tatacggttt ccggccaaac ggaaagtttg ccggaaatat ccgaacccga
    30121 catggcatac cgctgaccgc cccgccttcc cggcaaacac cctgaaagtc aaatgccgtc
    30181 tgaaacaaac acgcttcaga cggcattttc attctcaaac ctaatcgttg gtatttgccg
    30241 ttacctcttc caatgaagta atgccctgca taactttcaa aataccggcc cggcgcaaat
    30301 ccaccatacc ctccttatag gcaacgtcca aaatatccac ttccgtaccg ttgttcataa
    30361 tcacacgctg catttcttcg ctgatgggca taacctcata cacgcccgca cgccccttat
    30421 aaccctgccc ccggcaacgg tcgcaaccga cggcgcggta aagtttccaa tcttttgcaa
    30481 ggtcctcatc ggtgaagccg acttccttca aagcagaggc agacgggcgt tccacttcct
    30541 gtttgcagct cgaacacagc ctgcgtaaaa gacgctgcgc cataatcagg ctgaccgaac
    30601 tggcaatatt aaacggcgcg acacccatat tcagcatacg tgacaacgtc gccggcgcat
    30661 tattggtgtg cagggtggaa aacaccatat gccctgtttg tgccgcctta atcgcaatat
    30721 cggcagtttc caaatcacga atctcaccga ccataatgat gtccgggtcc tgacgcagga
    30781 aagacttcaa agcagcggca aaagtcaggc cctgcttatc attgacgtta acctgattga
    30841 tgcccggcag gttaatctcg gcagggtctt ccgccgttgc aatatttacc gactccgtat
    30901 tcaaaatatt caaacaggta tagagcgaca ccgtcttacc cgaacccgtc ggaccggtta
    30961 ccagcaccat cccgtaggga cggtgaatcg cttccaacaa caattttttc tgaaacggct
    31021 caaaaccgag ctggtcgatg ttcaaagacg cggcatcgga attcaaaatc cgcatcacga
    31081 ccttttcgcc aaacagcgtc ggcaatgtgc tgacacggaa atcgacaggc ttgccgccct
    31141 tttgaaaggt cagctgcatc ctgccgtcct gcggtatccg tttttcggaa atgtccaaac
    31201 gcgacattac cttaatccgg gaagcaagct gcccccttac cgcaatgggc ggctgaacca
    31261 cctcacggag ctgcccgtcc acacggaaac ggatacgggc attgtgttcg taaaactcga
    31321 aatggatgtc cgatgccccg ctgcgcaagg catccgacaa agtcttatgg ataaacctcg
    31381 gaacagggcc gtcttctgcc tcctcgttgt cgatatacag ggtgtggctt tcctcttcct
    31441 cctgcccctc cccaagctcc tgaagcagcg atgtcgaacg cgaacccacc caatcgagca
    31501 aacccgccaa ctggtcatcc tcgacaatga ccaattcaac ctcaatccct gcggcagaaa
    31561 cggttttctg aatttgcggc atctgtgtcg gatcggaaac cgcaaaaaat actttgtcgc
    31621 cccgacggaa aaccggcaca cagtggaact ccaccatctg ctcctccgtc aacaccccca
    31681 tcagcaccct gtggcgcgga taatgacgca aatcaagaat cgaataactg aacaccctcg
    31741 caatcaatgc cgcaagcgac ttgggcgaaa tgacaccgtc tgaaaacagc atcggcaaca
    31801 cttccttacc cgcctgcgac tcattgtagt aatgctcagc ctgctcaaca gtaaccacct
    31861 ggttttgaac cagaatcctc agcaaaccta cgctcatacg accttatccc caaatttatt
    31921 cattgttata cctgtacag
//
LOCUS       DAXIEB010000010        30897 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000010 DAXIEB010000000
VERSION     DAXIEB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.010"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(251..1510)
                     /gene="rho"
                     /locus_tag="ACFOA3_000399"
     CDS             complement(251..1510)
                     /gene="rho"
                     /locus_tag="ACFOA3_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6897459.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1730..4114)
                     /gene="ppsA"
                     /locus_tag="ACFOA3_000400"
     CDS             complement(1730..4114)
                     /gene="ppsA"
                     /locus_tag="ACFOA3_000400"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6897460.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4525..5346
                     /locus_tag="ACFOA3_000401"
     CDS             4525..5346
                     /locus_tag="ACFOA3_000401"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6897461.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5585..6247)
                     /locus_tag="ACFOA3_000402"
     CDS             complement(5585..6247)
                     /locus_tag="ACFOA3_000402"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6897462.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(6285..7112)
                     /locus_tag="ACFOA3_000403"
     CDS             complement(6285..7112)
                     /locus_tag="ACFOA3_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6897463.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            7390..8013
                     /gene="lolA"
                     /locus_tag="ACFOA3_000404"
     CDS             7390..8013
                     /gene="lolA"
                     /locus_tag="ACFOA3_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6897464.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8286..9425
                     /locus_tag="ACFOA3_000405"
     CDS             8286..9425
                     /locus_tag="ACFOA3_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6897465.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTGQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLLPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9558..9869
                     /locus_tag="ACFOA3_000406"
     CDS             9558..9869
                     /locus_tag="ACFOA3_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6897466.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKTTNNKQQTTNNKQQTTNNKQQTTNNKQQTTNNKQHQIPNTKHQIYIS
                     G"
     gene            9856..10635
                     /locus_tag="ACFOA3_000407"
     CDS             9856..10635
                     /locus_tag="ACFOA3_000407"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6897467.1"
                     /translation="MYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINCQVLLDFLSK
                     DDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSACNK
                     IIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSKTGTV
                     SEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIACDADIKRRA
                     YHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            10676..11206
                     /locus_tag="ACFOA3_000408"
     CDS             10676..11206
                     /locus_tag="ACFOA3_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6897468.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11223..11354)
                     /locus_tag="ACFOA3_000409"
     CDS             complement(11223..11354)
                     /locus_tag="ACFOA3_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6897469.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGHLVRVNLGLT"
     gene            complement(11373..12968)
                     /locus_tag="ACFOA3_000410"
     CDS             complement(11373..12968)
                     /locus_tag="ACFOA3_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6897470.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13075..13470)
                     /gene="hisI"
                     /locus_tag="ACFOA3_000411"
     CDS             complement(13075..13470)
                     /gene="hisI"
                     /locus_tag="ACFOA3_000411"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6897471.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(13501..14268)
                     /gene="hisF"
                     /locus_tag="ACFOA3_000412"
     CDS             complement(13501..14268)
                     /gene="hisF"
                     /locus_tag="ACFOA3_000412"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6897472.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14281..15018)
                     /gene="hisA"
                     /locus_tag="ACFOA3_000413"
     CDS             complement(14281..15018)
                     /gene="hisA"
                     /locus_tag="ACFOA3_000413"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6897473.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15051..15689)
                     /gene="hisH"
                     /locus_tag="ACFOA3_000414"
     CDS             complement(15051..15689)
                     /gene="hisH"
                     /locus_tag="ACFOA3_000414"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6897474.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(15820..17322)
                     /gene="pta"
                     /locus_tag="ACFOA3_000415"
     CDS             complement(15820..17322)
                     /gene="pta"
                     /locus_tag="ACFOA3_000415"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6897475.1"
                     /translation="MAKVLIVPVSTGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(17507..18565)
                     /gene="fbpC"
                     /locus_tag="ACFOA3_000416"
     CDS             complement(17507..18565)
                     /gene="fbpC"
                     /locus_tag="ACFOA3_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6897476.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(18586..20103)
                     /gene="fbpB"
                     /locus_tag="ACFOA3_000417"
     CDS             complement(18586..20103)
                     /gene="fbpB"
                     /locus_tag="ACFOA3_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6897477.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVFVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20171..21166)
                     /gene="fbpA"
                     /locus_tag="ACFOA3_000418"
     CDS             complement(20171..21166)
                     /gene="fbpA"
                     /locus_tag="ACFOA3_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6897478.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(21417..21887)
                     /locus_tag="ACFOA3_000419"
     CDS             complement(21417..21887)
                     /locus_tag="ACFOA3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6897479.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFERHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(21945..23321)
                     /gene="argH"
                     /locus_tag="ACFOA3_000420"
     CDS             complement(21945..23321)
                     /gene="argH"
                     /locus_tag="ACFOA3_000420"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6897480.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITIIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(23340..24209)
                     /gene="galU"
                     /locus_tag="ACFOA3_000421"
     CDS             complement(23340..24209)
                     /gene="galU"
                     /locus_tag="ACFOA3_000421"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6897481.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24237..24836)
                     /gene="rdgB"
                     /locus_tag="ACFOA3_000422"
     CDS             complement(24237..24836)
                     /gene="rdgB"
                     /locus_tag="ACFOA3_000422"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6897482.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(24829..25062)
                     /locus_tag="ACFOA3_000423"
     CDS             complement(24829..25062)
                     /locus_tag="ACFOA3_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6897483.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25170..25703)
                     /locus_tag="ACFOA3_000424"
     CDS             complement(25170..25703)
                     /locus_tag="ACFOA3_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6897484.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(25805..26263)
                     /gene="nudB"
                     /locus_tag="ACFOA3_000425"
     CDS             complement(25805..26263)
                     /gene="nudB"
                     /locus_tag="ACFOA3_000425"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6897485.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            26474..26550
                     /locus_tag="ACFOA3_000426"
     tRNA            26474..26550
                     /locus_tag="ACFOA3_000426"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26508..26510,aa:Pro,seq:ggg)
     gene            26708..28534
                     /gene="mafB"
                     /locus_tag="ACFOA3_000427"
     CDS             26708..28534
                     /gene="mafB"
                     /locus_tag="ACFOA3_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6897486.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSNNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            28537..29001
                     /locus_tag="ACFOA3_000428"
     CDS             28537..29001
                     /locus_tag="ACFOA3_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897487.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            29226..29393
                     /locus_tag="ACFOA3_000429"
     CDS             29226..29393
                     /locus_tag="ACFOA3_000429"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897488.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNDGLLYITTDHYISATRF
                     GAWKWEYNLCN"
     gene            29384..29671
                     /locus_tag="ACFOA3_000430"
     CDS             29384..29671
                     /locus_tag="ACFOA3_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6897489.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNKLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            30185..>30897
                     /locus_tag="ACFOA3_000431"
     CDS             30185..>30897
                     /locus_tag="ACFOA3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6897490.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDA"
BASE COUNT         7064 a         7447 c         8861 g         7525 t
ORIGIN      
        1 ttatacaaaa atgagcgcgg ttcggcgcaa ccttgaatca agttcccgca tcggttttca
       61 ttgccggtac ggatgcgttc aagccggctt tgcaaaggcc gcgctttcgg caagcggaca
      121 cggacactgc cgacggttgc gccgttaacg gggggaggga ggagctgcgc cgaccgtgtg
      181 aatgaaagtg ccgtctgaaa cccgattttc aggcttcaga cggcatttcg cattaatgcg
      241 ggcggcgcgt ttatttgccg cgcatcagtt caaagaaatc gtcgttgttt ttagaggctt
      301 tgattttccc gtttaagaac tcggtagctt cgatttcatc catcgggtgc aggaacttgc
      361 gtaagagcca catacgttgc aactggtcgt tcggcacaag cagctcttcg cggcgcgtgc
      421 cggatttgtt gatgttgatg gcggggaaga ggcgtttttc cgccatacgg cgatcaaggt
      481 gcaattccat attgccggtg cctttgaatt cttcgtaaat cacatcgtcc atacggctgc
      541 cggtttcaac caatgcggtg gcgataatgg tcagcgaacc gccttcttcc acgttgcgcg
      601 ccgcaccgaa gaaacgtttg gggcggtgca gcgcgttggc atcgacaccg ccggtcagga
      661 ttttgcccga ggtggggacg acggtattgt aggcgcgggc aaggcgggta atcgaatcca
      721 gcaggatgac cacgtctttt ttgtgttcca ccatacgctt ggctttttca agcaccattt
      781 cggcaacttg gacgtggcgt tgagccggct cgtcaaaggt ggaggagact acttcgccac
      841 ggacggagcg gctcatttcg gttacttctt cgggacgctc gtcaatcaag aggacgatga
      901 gttcgacttc gggatagttt gcggtaacgg cgtgggcaat attttgcagc atcacggttt
      961 taccgctttt gggcggggca accaagaggg cgcgctgacc tttgccgatg ggggaaatca
     1021 ggtcgatggc gcgtccggtc aggttttctt cggactttaa gtcgcgttcc agcttcaact
     1081 gttcggtcgg aaacagcggg gtcaggtttt caaacaggat tttatggcgg catacttccg
     1141 ggtggtcgcc gttgatggta tcaagcctga ccagggcaaa atagcgttcg ttgtcttttg
     1201 ggacgcgcac gcttccttcg atggtgtcgc ccgtatgcag gttgaagcgg cggatttggg
     1261 tgggcgagac atagatgtcg tcggggccgg caagatagga cgtgtccgcg ctgcggagga
     1321 agccgaagcc gtcgggcagg atttcaagcg tgccggagca ggtgaaaccc tcgccttttt
     1381 tcatcatctg gcggacgatg gcaaatacga ggtcttgttt gcggaatcgg ttggcgtttt
     1441 cgatgccgtg ttcttccgcc aattctaaga gtttggaaat gtgcagggtt tgtaattcgg
     1501 agacgtgcat aataatgatg tattttgaag aggaaaaaga caggcagatg ccgtctgaaa
     1561 gaagaagctg accgttgccg gttgctcggg gaagggggaa ttgtaggcag tcggcgcgtg
     1621 ggtgtcaaat attatcgcgg acggggcatc ggcaggaaat gccgtctgag cggagctgct
     1681 tggaaaaaaa tacccccgcg cttttcaggc tcgggggtat gggcattgat tatttgttca
     1741 attcattcgc caaatatagc caagtttcga tgacggtatc cgggttcagg gaaacgcttt
     1801 caatgccttc ctcaaccagc catttggcga agtccggatg gtcggacggg ccttgaccgc
     1861 agatgccgac atatttgttc tgcttgcggc aggcggagat ggcaagatgc agcatcactt
     1921 tgacggcagg gttgcgttcg tcaaacgatt cggataccaa gccgctgtcg cggtcaagac
     1981 cgagggtcag ttgggtcatg tcgttcgagc cgatggagaa gccgtcgaag tattgcagga
     2041 attgttccgc caataccgcg ttgctcggca gctcgcacat cataatcagg cgcaggccgt
     2101 ttttgccgcg ttccaagccg ttttctttca gggctttgac aacggcttcg gcttcgccca
     2161 aagtgcggac gaacggaatc atgatttcaa cgttggtcag acccatttcg tcacgaacgc
     2221 gtttcaaggc tttgcattcc aaggcgaaac agtctttgaa gttgtcggcg acataacgcg
     2281 ccgcgccccg gaagcccaac atcgggtttt cttcatgcgg ttcgtatacg ttgccgccga
     2341 ctaggttggc gtattcgttg gatttgaagt cggacatacg gacgatggtt ttacgcggat
     2401 aaaccgatgc ggccaatgtc gccacgcctt cggcgatttt atcgacgtag aagtcgacag
     2461 gggacgcgta accggcgata cggcgggtaa tttccgcttt taattcgtcg tcttgtttgt
     2521 caaattccaa caaggctttg gggtggatac cgatttggcg gttgatgata aattccatac
     2581 gcgccaagcc gatgccttcg ctgggcaggt tggcgaagct gaatgcgagt tcgggattgc
     2641 cgacgttcat catgactttt acaggtgctt tgggcatgtt gtccaaagca acatcggtaa
     2701 tttgtacgtc cagcaggccg gagtagatga agccggtatc gccttcggca caggatacgg
     2761 taacttcttg accgtttttc agcaattcgg ttgcattgcc gcagccgaca acggcaggaa
     2821 tacccagttc gcgcgcgatg atggcggcgt ggcaggtacg tccgcccctg ttggtcacga
     2881 tggcggaagc gcgtttcatc accggttccc aatccgggtc ggtcatgtcg gtaacgagta
     2941 cgtcgccggc ttcgacggaa tccatctcgg aagcatcttt aatcaggcgt actttgccct
     3001 gtccgacttt ctgaccgatg gcgcggcctt cgcataatac ggttttgtcg ccgttgatgg
     3061 cgaagcggcg caggttgcgg ctgccttctt cctgggattt gacggtttcg ggacgggctt
     3121 gcaggatgta gagtttgccg tccaagccgt cgcgtcccca ttcgatatcc atcgggcggc
     3181 cgtagtgttt ttcgatggtc agtgcgtaat gcgccaactc ggtgatttct tcgtcggtaa
     3241 tggagaagcg gttgcggtct tcttcgggga catcgacgtt ggttaccgat ttgccggctt
     3301 cggctttgtc ggtaaaaatc attttgatgt gtttcgaacc catggtttta cgcaggatgg
     3361 cgggcttgcc cgctttgagc gtgggtttga acacataaaa ttcgtccggg ttgaccgcgc
     3421 cttgtacgac gttttcgccc agaccgtaag aggaggtaac aaagacgact tgatcgtagc
     3481 cggattcggt gtcgagggtg aacatcacac ctgatgcgcc gctgtcggaa cgcaccatgc
     3541 gttgtacgcc ggcggaaagg gcgacgatgt cgtgttcgaa gcctttgtgg acacggtaag
     3601 aaatggcacg gtcgttatac agggaagcga atacatggtg catcgcttct ttaacgttat
     3661 ccaagccgtt gatgttcaag aaggtttcct gttgtccagc gaatgatgcg tctggcaggt
     3721 cttcggcagt tgcggaagaa cgtacggcaa cggaaatgtc cgcaccgccg gcatcggcaa
     3781 ccattttgtt ccatgccgct tcgatttcgg catcgagctg ttcagggaaa ggcgtgtcca
     3841 aaatccattg gcggatttct ttgccgacgc gtgccagttc ggcaacgtct tcgacatcca
     3901 attttgccag tgcggcggaa atgcgttcgc tcagaccgtt gtgtgcgagg aatgcgcggt
     3961 aggcatcggc tgtggtggca aagccgccgg ggacgcgaac gcctttttcg gtcagctgac
     4021 tgatcatttc gcccagcgag gcgtttttac cgcccacgcg ttcaacatct gtcatacgca
     4081 ggttttcaaa ccagattacg tagttgtcgg ccatttgtgt gtccaatcca aaatatgtta
     4141 aaaaagaaac aaatccgcgt gcttatttta agcgattcgt tcctctgctg tcatgtgttt
     4201 tatccgtttt aaaatcatga tgccgtctga aaaattgcgg tttcggcgtg tgtagcggtt
     4261 tgaaacttgc agccggtata cttctttttt tgggtatttt ctttgtaaaa caggtggttt
     4321 gaataggtta atgttttttc tgtttgattt ttttgtttat tttttaaaat tttctgccaa
     4381 aaaatacttt atataaataa ttttatttca aaattatatt gtgtctgttt gggtgtaatc
     4441 cgaggtaggc gtgctgcggg tgctttcctt gtgtctgccg ctgctgttat gatgatattt
     4501 ttattctgta tttaaggatg gaagatgagc agtccgcgcc atgtgtttta catttccgac
     4561 cgtaccggtc tgactgccga gaatatcggc gaggctttgc tgaaccagtt tggcaatctg
     4621 tcgttcaaac gccatacgca tccgtttgtc gatacgccgg aaaaggcgcg cgcggtggtg
     4681 gagaaggtca atcggagccg gcaggaaaac ggtcagcgtc cgattgcgtt tgtcagtgtg
     4741 gttgatgacg aaatccgtcg gattatcaaa ggggcggatg cttttcagat taatttcttt
     4801 gagacttttt tgggactgtt ggagaaagaa ctcaataccg aagccacggc atccgagcag
     4861 gggcatcaca gtatcggtaa tacgaagcgt tacgatgcgc gtatggaggc ggtgaatttc
     4921 tcattgaacc acgatgacgg ggtcagcgat aagaaccttc aggaagcgga tgtaatcttg
     4981 atgggtgtat cgcgttcggg caaaacgccg acctgccttt acctcgccct gcaatacggc
     5041 atccgtgcgg caaactatcc gctgattccc gacgatttgg aatcggccga tctgccgcgt
     5101 atggtcaagc cttataggga taagctgttc gggttgacca tccagccgga acgtttgcag
     5161 gccatccgcc aagagcgccg cccgaattca acttatgcca aaatcgatac atgccgcagc
     5221 gaggtggcag acgcgcagag tatgttcaga cggcatggca ttccgtttgc gaatacgacg
     5281 gataaatcag tggaagagct tgccgtacac atccttcagg cgtgcaagct caaacgcagg
     5341 ttttgacggg ctttgattcg gtttgaaggc ggaaatgccg tctgaaatca ggtttcagac
     5401 ggcagtttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
     5461 aatagtacgg agccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
     5521 taaggcgagg caacgccgta ctggtttttg ttaatccact atatgtttgt ggggcggata
     5581 tttttcagga ctgtattttg tccagacatt cgagcagatc gagcggcgtg cggatgtgga
     5641 aatccgcctg ccatgagccg gtatcgtctt cgggagcgat gtagccccat tcggcgagga
     5701 cggttttcat gccggcgttg cggcctgctt ggatgtcgcg ttcggcatca ccgacgtaga
     5761 gtgtgtgttg cgggtcggcg tggatttgtc cgcacgcata cagcatgggt ttgacgctgg
     5821 gcttgggttc gccgcaggtg tcgccgctga cgacgacggc gggtgggatg atgaagccga
     5881 gtttggggac gagtttgtcg gtgaagcgca tgggtttgtt ggtgatgatg ccccatttga
     5941 tgccgcgttg gttgagttcg gcaatgagtt cgttcacgcc gtcaaagagc tcggtgtctt
     6001 gggcgtagcg gcggtcgtat tcctccagat attcggtgcg ccatcgggca tagtcggggt
     6061 ggtcgggggt gatgcctgtg ccgagcttga gcagtcctgc cgcgccgtgg ctggcttggg
     6121 tgcggatctc gtccatgctt tttgcaggta gtccgtggcg agcgagcagg gtgttgagtg
     6181 cgcctccgag gtcgagggcg gtgtcggcaa gtgtgccgtc gaggtcgaag agtacggcgt
     6241 gtatcatgtg tgttcctttt ttataaagtg cgggacgaag ggtttcagac ggcatgttta
     6301 ttttgtttca aaccctgttc gaaatcttcc agcatatcca attcaaagcg gctgaagccc
     6361 gctttttcgc gcgcttcgat gttcacatag ccgcggaaga taaacatatc gtaacgggca
     6421 atcagactgc ggaacagggt aatcggttcc aaaccgcgtt cgcggcagag gtgttgatac
     6481 caatggttgc cgatggcgac atgacccact tcgtcgcggt aaatgatgtc caatacgccg
     6541 caggtttccg aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt aacgtccagc
     6601 ccgcgcgctt ccaacacgcg cggtaccaac gccatacgca acagcgggtc gtaggcggtt
     6661 ttgtacgcca tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc gaagccgaaa
     6721 gcgcgcaatc tgtcgcgcat caggcggaaa tggtacacct cttccttcgc cactttcacc
     6781 cagtcgcgga caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc caaagccaga
     6841 ttgacggcgt tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata gccttctgcc
     6901 gtgttcattt tgcgtggcgt cagttgcgac ggcgcgacca aaacaggctt gtccggccgt
     6961 cccgcgcggg ggaaatctgc cggtggcgcg tttgcttccg ccccgtccgc attttgaacg
     7021 gcggcaaacg cctcatccgt cagccgtcct ttttcatctg ggtcgcccga aagcagggcg
     7081 cgttccagca aagcataaat atcgagtttc atctcaagtc cgccgtgttc ggaaaacaaa
     7141 tattatagcg ttttaaaaaa acaagatgag gcatataatc tccgcgattc ggcattccgc
     7201 gcccaaaccg tcaaatcacg cggcacacaa ttaaagggca gcgtggcgcg cctacctttt
     7261 ccgagcggtc aaaaaaatca gccctcggaa aacgctgttt gtaaaatgca aaccgcccgt
     7321 aacgccgccc gtatgattgt tttgccgcgc cgatacttta cgccacaccc atcccaacaa
     7381 ggaaaaataa tgatgaaacc gcacaacctg ttccaatttc tcgccgtttg ctccctgacc
     7441 gtcgccgtcg cttccgcaca ggcgggcgcg gtagacgcgc tcaagcaatt caacaacgat
     7501 gccgacggta tcagcggcag cttcacccaa accgtccaaa gcaaaaagaa aacccaaacc
     7561 gcgcacggca cgttcaaaat cctgcgcccg ggcctcttca aatgggaata cacttcgccc
     7621 tacaaacaga ctattgtcgg cgacggtcaa accgtttggc tctacgatgt cgatttggca
     7681 caagtgacca agtcgtccca agaccaggcc ataggcggca gccccgccgc catcctgtcg
     7741 aacaaaaccg ccctcgaaag cagctacacg ctgaaagagg acggttcgtc caacggcatc
     7801 gattatgtgc tggcaacgcc caaacgcaac aacgccggct accaatacat ccgcatcggc
     7861 ttcaaaggcg gcaacctcgc cgccatgcag cttaaagaca gcttcggcaa ccaaacctcc
     7921 atcagtttcg gcggtttgaa taccaatccc caactctcgc gcggcgcgtt caagtttacc
     7981 ccgcccaaag gcgtggacgt gttgagcaac tgatgccgtc cgccccgatg ccgtctgaaa
     8041 gccgccgagg cttcagacgg cattttcacg caggcggaac aatgtcccgc attaccgccc
     8101 gatcgggcat cggaacggca aacccgtgaa aattaacggt tgcgccccgg ttgtttttgc
     8161 cgtttaatgc aaaccttgct gcatcaaggg ccaagaaagc cgaccggccg cccccacagc
     8221 ttccgatgca ggcggccagg ccgtccctgc aatgtttttt atttttgaac gaaaggtcga
     8281 aaaccatgaa aaaaacactg gtggcggcgg caatcctgag cctcgccttg actgcgtgcg
     8341 gcggcggaag cgataccgcc gcccaagccc cctccgccaa gcctgaagcc gaacaatcgg
     8401 gcaaactcaa tatctacaac tggtcggatt atgtcgatcc cgaaaccgtc gccgcctttg
     8461 aaaaagaaac cggcatcaag acgcgttccg attattacga cagcaacgaa acactggagg
     8521 caaaagtcct gaccggcaaa tccggctacg acctgaccgc gccgtccatc gccaacgtcg
     8581 gccggcaaat caaagcgggc gcgtatcaga aaatcgacaa ggcgcaaatc ccccattacg
     8641 gcaacatcga taaagatttg ctgaaaatga tggaagccgt cgatccgggc aacgaatacg
     8701 ccgtccccta tttctggggc atcaacacct tggcaatcaa cacggggcag gtgcaaaagg
     8761 cattgggtac ggacaagctg cccgaaaacg aatgggattt ggtgttcaaa cccgaataca
     8821 ccgccaaact caaatcctgc ggcatcagct atttcgacag cgcaatcgaa cagattccct
     8881 tggcgttgca ctatttgggc aaagacccca acagtgagaa tcccgaagac atcaaagccg
     8941 ccgtcgatat gatgaaagcc gtccggggcg acgtgaaacg cttcagctct tccggctata
     9001 tcgacgatat ggcggcaggc aacctgtgtg ccgccatcgg ttacggcggc gatttgaaca
     9061 ttgccaaaac ccgtgccgaa gaagccgcaa acggcgtgga aatcaaagta ttgaccccga
     9121 aaaccggcgt gggcgtgtgg gtggattcct ttatgattcc gcgcgacgcg caaaacgttg
     9181 ccaacgccca ccgctatatc gactacacgc tcctgcccga ggtggcggcg aaaaacggca
     9241 gcttcgttac ctacgcaccc gccagccgcc ccgcgcgcga gctgatggat gaaaaataca
     9301 cctccgatgc atcgattttc ccgaacaaag aactgatgga aaaaagtttc atcgtatcgc
     9361 ccaaatccgc agaatccgcc aaactgggcg tgaagctgtg gcaagggctc aaagcgggca
     9421 aataaccgca acccctgccg tctgaaacct ttcgggcggc aggaaacggc gcgtccgtta
     9481 tcaaacaggg gggcgtttcc cctcctgccg gttatgattg ggttaagatt aaaatgattt
     9541 agtaaaatga gaaagatatg gatttaagta tcgtagttcc catttataat gtcgaaagtt
     9601 atttggaagc gtgtttaagt tccatagaac ctatattaag taatgaaaat gtcgaactta
     9661 tccttgtgaa tgacgggtca aaagacggaa gtgaagatat atgttataaa tatatagata
     9721 aaacaacaaa caacaaacaa caaacaacaa acaacaaaca acaaacaaca aacaacaaac
     9781 aacaaacaac aaacaacaaa caacaaacaa caaacaacaa acaacaccag ataccgaaca
     9841 ccaaacatca aatatatata tcaggataac caagggacgg cagaagctag gaataccgga
     9901 ataaaaaatt caaacggaaa atatatagtc tttattgatt cagatgattt tattaattgt
     9961 caggtcttgc tggattttct tagtaaagat gatattgata tgccggatgt ggttttttta
    10021 aatgcggtta aatatgataa gggaagtgtt tcacattttg gcgaagatta tcagcctgaa
    10081 aaaatactca atcaatccaa agtcgaagtt ttgaaaggat tatgccgatt tagaaaattt
    10141 ccgggttcgg cgtgtaataa gattataaaa agagaattga ttatcaaaga aaaactattt
    10201 tttgaaaaag aggtttatgc cgaagatatc gaatggtcaa tgagattatt taatgcggca
    10261 acaacttttt cttatttgga cggttgttat tactattatc ggcagggaag aaaagattct
    10321 aaaacaggaa ctgtttcgga aaaaagtata aagtcattat tatatatttt ggagaaaaat
    10381 gcggaaatgg aatttgatag ggatatatca agttatcttt attcttttct ttcctacgaa
    10441 tatctcgttt tgctttttat catgacgagt aaaaatatag cgtgtgatgc tgatataaaa
    10501 agaagggcgt atcatttaag gtttatgctg ttaaagtcca ataaattgat atataagctg
    10561 atattcccga taatcacatt attcggggtc gatattacag gcaggatttt aaaagcaatc
    10621 agggggaata tttaataaat cctttaacac tatacatctt actgaaggag gaaaaatgaa
    10681 cgcaatccga actttccaaa accgcacgcc cgaaatccac gaaagctgta tgatagacga
    10741 agcgtgcgtc gtcatcggcg aagtgtcgct tgccgaagac gtttctgtgt ggccgtgcgc
    10801 cgtgttgcgc ggcgatgtga acagcatcac cgtcggcgcg cgcagcaata tacaggacgg
    10861 cagcgtcctg cacgtttccc acaaaaccgc cgccaaaccc gaagggtcgc cgctggttat
    10921 cggcgaagac gttaccgtgg ggcacaaagt gatgctgcac ggctgccgta tcggcaaccg
    10981 cgtcctggtc ggcatgggga cgacggttct ggacgatgcc gtgattgagg acgaagtgat
    11041 gatcggcgcg ggcagcctcg ttccgccgcg caaacgtttg gcgggcggct acctttatgt
    11101 cggttcgccg gtcagacaag tgcgcgtgct gaccgatgag gaaaaagcct ttttgaaata
    11161 ttccgccgcg cattatgtga agctgtcgaa acagtacggg atgtgaaatc acatcggcgt
    11221 tcctacgtca gccccaaatt cacgcggacg agatgcccga taacggtatc cgatgcgcct
    11281 tgattttgac cgtctgcgtt tgagttgcag gcaaaaatgc cgtctgaagc ccttttttcg
    11341 ggtttagacg gcattttatt gccgattgtt ttttaaagtt tgaccgaatg ttcgcgcgtt
    11401 tcgtggaaca cgatgtccgg ccaacgttct tgcgtcaacc ccaaattcac gcggttgggg
    11461 gcgaggtagg cgaggttgcc gcccgcgtcg atggcgaggt tgcccgcgtt ggctttttca
    11521 aactccgcca gttttttctt gtcgtcgcac gatacccagc gcgccgacca gatggatgcg
    11581 ctgtcgaaca cggcttctac gccgtattcg ttggcgaggc gcgaggttac gacttcaaac
    11641 tgcaacacgc cgaccgcacc caaaatcaaa tccgcgccgc tcatcggttt gaacacctgt
    11701 accgcgcctt cttcgccaag ctgttgcaaa cctttttgca gttgcttgat tttcagcggg
    11761 tttttgatgc ggacgctgcg gaacagttcg ggcgcgaaga agggaatgcc tgtaaatgcc
    11821 agttgttcgc cttcggagaa gctgtcgccg atttggatgt tgccgtggtt cgggatgccg
    11881 ataatgtcgc cggcgtaggc ttcttcaacc agctcgcggt cgtgggacat gaaggttacc
    11941 acgctggagg cggcgatttc gcggttgata cgcaggtgtt tcattttcat gccgcgctcg
    12001 aatttgccgg agcagacgcg caagaaggca atacggtcgc ggtgtttcgg gtccatattg
    12061 gcttggattt tgaagataaa tccggaaaac ttcggctcgt ccggctcgac catacgcacg
    12121 gtcgcatcgc gtggtttcgg cgcgggcgcc cattcaatca atgaattgag gatttcctga
    12181 ataccgaagt tgttaatcgc agagccgaag aatacgggcg tgagttcgcc ggcgaggaat
    12241 tcgtcgagat tgaactcgtt ggaagccgcc tgcaccaatt cgatttcgtc gcgcaactgt
    12301 tggatttcca atggaaagcg ttgttccaat tcgggattgt ctatgccttt gatgatgtcg
    12361 aactcgtgcg gcaggcgttc gccgcccgct tcaaagagat agatttcgtc gttcaggatg
    12421 tggtatacgc ctttgaagtt tttgcccata ccgatcggcc aagtaacggg cgcgcagcgg
    12481 atttgcagga tgttttccac ttcgtccaag agttccagcg aatcgcgcac ttcgcggtcg
    12541 tatttgttca taaacgtaac aatcggcgta tcgcgcaggc ggcagacgtt taagagcttg
    12601 atggtttgcg cttccacacc ttttgccgcg tcaatcacca ttaatgcgct gtccacggcg
    12661 gttaaaacgc ggtaggtgtc ttcggagaag tcttggtgtc ccggcgtgtc caagaggttg
    12721 acggtgtggt ctttatagtc gaactgcatc acgcttgatg ccacggaaat gccgcgctgc
    12781 ttctcgatgt ccatccagtc ggaagtggcg aatttgccgg tttttttgcc ttttaccgta
    12841 cccgcgcttt gaatcgcacc tgaaaacagc aagagttttt cagtcaacgt ggttttacct
    12901 gcgtcagggt gggagatgat ggcaaacgtg cggcggcggc gcacttggtc gaggatttct
    12961 tgggacatgg ttttctttgc aaaaaggttc aggccgcttt tcagacggcc cggacagtgt
    13021 ttgagacggc gaaattgtac aaaaaaatgc ctgataattc aatgttggag gcggtcagtg
    13081 cgtgctgccg taaatctctt tttcgtcttt caggacggca tcggcggttt cccacgcgct
    13141 gccacgccag actttgtaaa agcagctttc tcgcccggtg tggcaggcga tgccgccgtt
    13201 ttgggcgatg agcatcacaa tggcgtcgcc gtcgcagtcg aggcgcagtt cgcggacttt
    13261 ttgcgtgtgt cccgactctt cgcccttcat ccattgtttt tggcgcgaac ggctgtaata
    13321 gtgggcaaag ccggtttcga cggttttttg cagggcttcg gcgttcatcc acgccaccat
    13381 taaaacacgt ttggtttcgg catcttgggc gatggcgcaa accaaacctt tttcgtcaaa
    13441 tttgacggct tcaagcaggt ttttatccat attttctttc agacggcatg gtcggggcgg
    13501 tcagaggcgc acttcgatgc cggcttcgcg catagcgcgt ttggcttcgc ggatggcgat
    13561 ttccccgaaa tggaaaacgc cggcggcaag tacggcatcg gctttgcctt cggttatgcc
    13621 ttcaatcagg tgccggacat tgccgacccc gccggaggcg atgacgggga tgtcgacggc
    13681 ttcggcaacg gcgcgggtca gcggcaggtt gaaaccctgt ttcgtaccgt ccctgtccat
    13741 accggtaagc aggatttcgc ccgcgccgcg tttttgcatt tcgatcgccc attccaccgc
    13801 atccaaaccg gtcggattgc gcccgccgtg ggtaaagatt tcccagcgtg tgtttccggg
    13861 gttgacggct ttggcatcga cggcggcgac gatggcttgc gaaccgaaaa atccggcggc
    13921 ttcgtcaatc aggtcgggac gggtaacggc ggcggtgttg atgctaactt tgtccgcgcc
    13981 ggcattgagc aggcggcgga tgtcggcaac ggtgcgtacg ccgccgccga cggtcagggg
    14041 gatgaagact tgtccggcaa cttcttcgat gatgtgcagg atggtgtcgc ggttgtcgga
    14101 tgaggcggtg atgtcgagga aggtcaattc gtccgcgcct tcgccgttgt agcgtttggc
    14161 ggcttcgacg gggtcgcccg cgtcgcgcaa accgatgaag ttcacgcctt tgacgacgcg
    14221 cccgtctttt acgtcgagac aggggatgat gcgttttgcc agtgccataa tcggatgcct
    14281 ttagtcgagg gaatctgcca gttgctgcgc ttgggcaaaa tcgatgctgc cctcgtaaat
    14341 cgcgcggccg gtaatcgcgc ctgctacgcc atgtttttcg gcggcacaca gggcgcggat
    14401 gtcgtccaag ccggtcagtc cgccggaggc gatgacggga atgcggacgg cttgggcgag
    14461 tttgaccgtc gcgtcgatgt tcacgccgct catcataccg tcgcgcccga tgtcggtgta
    14521 gatgatgctg ttgacaccgt cgtcttcaaa gcgttttgcc aaatcaatta catgatgccc
    14581 ggttacggtt gcccagccgt cgatggcggc cataccgtct ttggcatcca gcccgacaat
    14641 aatcctgccg gggaaggctt tgcacgcctc gcgcacgaag tcggggtttt tgaccgccgc
    14701 cgtgccgata atcacgtcgt tcagacccaa atccaaatat tgtccgatgg ttttcaaatc
    14761 gcgtatgccg ccgccgagct gtacggggat gtctttggcg acggcggcaa ggatgtcttt
    14821 gatggcgggc aggttttgcg gaacgccggc aaacgcgccg ttcaaatcta ccagatgcag
    14881 gcggcgcgcg ccttgtttga accagtgcag tgcggtttcg gcgggcgaat cggaaaagac
    14941 ggtcgcctct tccatcagcc cttgtctcag gcggacgcag cgtccttctt tcaaatcgat
    15001 ggcgggtatc agcagcataa tttttctcct tgtgcggggc cgtgtccggc ttaccagttt
    15061 aaaaagtttt tcaacatcgt cagcccggca tcgtggcttt tttcggtgtg aaattgcgtg
    15121 gcgaatacgt tgtctttgcc gacgatgcag gcaaacgggg acgggtagtc gctttcgccc
    15181 aatatggttt cgggattttc gggggcgaaa tagtagctgt ggacgaagta aaaacgcgtg
    15241 tcttggggaa tatctttaaa cagcgggtgg ttttgggttt ggcgcacggt gttccagccc
    15301 atatgcggga ctttcagacg gcatccctgc gggtcgcgga ggtcgcgctc aaagcgtctg
    15361 actttgccgc cgaaccagcc caagccgtcg gtgtttcctt cttcgctgtg gtcgaataaa
    15421 agttgcgcgc cgacgcagat tccgaaaaag ggtttgtttt ttaaggcatc tttgactgcc
    15481 tcgtccaagc cgtcgcgcgt cagtgccgcc atacagtcgg gcatcgcgcc ctgaccggga
    15541 aaaatgactt tgtcggcgcg ggacacgcgt tcggggtcgc cgcttaaaaa gatttcggta
    15601 ttttttccgg caagctgccc cgccgtccgg acggatttca atacggaatg caggttgccc
    15661 ataccgtaat cgataatggc gatttgcatg gcttcctcct ctttttttgc aatatggctg
    15721 cgattttaac aaacaaatgt gccgtgctga taaaaatgcc gtctgaaaac gggagtctgt
    15781 cttcagacgg catagggttt aaacccggaa agccgtttgt cagccttcca tttgttttgc
    15841 ctgaacggca gtcagggcga tggtgaacac gatgtcttcc accagcgcgc cgcgcgacag
    15901 gtcgttgacc ggtttgcgca ggccttgcag cagcgggccg acgcttaaga cgttggcact
    15961 gcgttggacg gctttatagg tgcagttgcc ggtgttcagg tcggggaaga ccaaaacggt
    16021 tgcctgtcct gccaccgggc tgcccggagc tttggatttg cccacgcccg gcacggttgc
    16081 cgcatcatat tgcagcgggc cgtcgatggc gaggtcgggg cgtttttccc gggcaagttt
    16141 ggttgcttcg atgacgatat cgacatcggg gccgctgccg gagttgacgg tggagtagga
    16201 aatcatcgcc actttcgggt cgatgccgaa ggcttttgcg gaatcggcag actggatggc
    16261 gatgtcggca agctgttgcg cggtcgggtt cggattaacc gcgcagtcgc cgaagacgag
    16321 gacttggttg ggcagcagca taaagaatac gctggacacg aggcttgcgc ccggtgcggt
    16381 tttaatcagt tgcaaagcgg ggcggatggt gttggcggtg gtgtgaaccg caccggatac
    16441 caaaccgtcc acatcatttt gcgccatcat catcgtaccg agtaccacgg tgtcttgcag
    16501 ttgcttgcgc gcgtcttcgg gtgtcaggcc tttggatttg cgcagttcgc acatcggctc
    16561 gacgtattgt tcgaccaatg aggcgggatc gatgatttcc aaagagtcgg gcaggctgat
    16621 gccgcgttct ttggcaacgg cttcgacttc ttcgcgtttg gcaagcagga cgcagcgggc
    16681 aatgcctttt tcgtggcaga tggcggcggc ttggacggtg cggggttctg cgccttcagg
    16741 caggacgatg cgtttgtcgg cttggcgggc gaagtcgatc aggttgtagc ggaattgcgc
    16801 cggcgacagg cgttttgctt cgcggcctgc caatacggat acgtctttca gcgcgtcgct
    16861 cgaaccgaag aaggtcaggc cggttttttc ggctgccgct tcggcaacgg aggctgccgc
    16921 gccgtccacg acaaaacctt ccaatacgcc cggcgcggcg gcgaagaact gtttggcaag
    16981 gttcacccga tttgccagtt cgtcggcatc ggcgttgtcg gaacggacgg cgaagacggc
    17041 tgccgcgtca agggacaatg ccagttcgac gtttttgcct gcgaggtaga ttttgtcggc
    17101 atcgggcgcg atgccttcga tgacgaggtt ggcggcatcg agtgcggcaa ctttgccgac
    17161 cagtgcgtcg aaccagtcgt cgcttttgcc ttgcgcgagc agggtttcgg cggttgcgtc
    17221 aacggcttgg aaaatttgtg cgtccagtgc ttttgcaaag gcttgtgcgg cggcggaggc
    17281 gtccagtccg gtagatacgg gtacgatgag tacttttgcc atgatatatc ctttcgtatg
    17341 ctgcggtgtg cggcatatgt ggttggaagg ggcggcatat aggcagaaac ggctgcctgc
    17401 gtgccgtgcg tgccgtgttt ggcttgaggc gcgcaggttg aatatagcaa acaaattctg
    17461 tttccaacaa gataaatatc cgcaggcttg tggatgctgc cgcctttcag agggtatttc
    17521 cggggaagaa cagggcggga ccgtccaaat ggaggacggc ggaaatgccg tctgacaggg
    17581 tgggggcgga agggaggttg agcgtgagga cggtttgtcc gacccggaga ctgatttcgg
    17641 tatgccgcgc tttgggcgtg gttttgagaa ccacggcgtg aatggaggcg gtgggtgcgg
    17701 aatgggggtg aaggctgaac tgttccggac ggatgagcag tgtgccgcgt gtgcctgcgg
    17761 gtgcgccgct ttgaacgggc aggcggccca atccgcaatc ggcggtgccg tcggcgttga
    17821 gcgcggcggg gaacacgatg ccttcgccga taaacagggc ggcatcaagg tcggcaggtt
    17881 gtcggtacaa ttcgtgaggg cttgcagttt ggaggatgcg cccctgtttc atcacggcaa
    17941 tccggtcggc gtattgcagg gcttcttcgc ggtcgtggct gacgaaaacg gcagatttgc
    18001 cgttggcgcg cagggcggca atcatgtctt cgcgaatctg gcggcgcaac tgttcgtcca
    18061 gcgcgctgaa gggttcgtcc aacaaaatca gttcgggatc gggtgcaagg gcgcgggcga
    18121 gggcgacgcg ctgttgctgt ccgcccgaaa gttcgtgcgg atagcgtccg gcaagttcgg
    18181 aaatgccggt caattccaac atagcttcga tgcgctgccg ttcttgcgcc gtcttgcctt
    18241 tcccgttgcc cagcccgtag gcggtgttgc ggtaaacggt caggtggggg aacagcacac
    18301 cttcctgtac gacataaccc aaacggcgtt cgcggacggg gaggttggta tttttcgaga
    18361 agatggttct gccggaaagc gaaatttcgc caaaatcggg ttgttcaaaa ccggcaaggc
    18421 agcgtaaaag ggtggttttg ccgcagccgg acgcgccgac gataaagagg atttcgcccg
    18481 ggtcgaggct gagcgaaatg tcgtttaaaa ctggggtgtt ttgaaaactt ttggacaggt
    18541 gtccgatgtg cagggcggcg gtcatggcgg tacttcctca agctgttatt tgaaggcgta
    18601 tttcttcagc aggaatacgg ggatgccgga aaataatacc agcatcagcg cgtaaggggt
    18661 ggcggcggcg tattgtgcgt ccgatgtgta ttcccaaacg gcggtggaga gtgtgtggac
    18721 atcgtcggtg gtcagcagca gggtggcggt cagctctttc atcagtttga ggaagacgag
    18781 tgcgaatgcg gcggtaatgc cgggcaggat ggacggcagt accaacgtcc tgaaaataaa
    18841 gaagtgtccg cgccccaatg ttgcgccgac ctgttccatc cctttgggga gttgttccaa
    18901 ggaagtcctc agggtggttt gcgccatcgg caggtaaagc atgaaatagg caaggatgac
    18961 gacgataaag gtttggtaaa cggcaggggt gtagttgatg ctgaaataaa ccaaggatag
    19021 ggcgataacc aaaccgggga cggcgtgcag taaaaacggc agcctgtcta tccaaacggt
    19081 taaaaaattg cgatagcgaa ccgatgccca aacaaggggc aaggcacata atatagtcaa
    19141 aatcgcacct aaagccgata cgcttaagga acggataaag gcatcaaata cgaatacgag
    19201 cgcgaatgtg ccggaagtgc cgaccatcat ccaatgtatc aatacgccaa aggggataat
    19261 aatgcccaaa gtcaacaagc tgcttaaaaa aacaatcgcg ccgatctgac cgggcagttt
    19321 gagggttttg acgggataag gacgggcaac gcctttgccg ctgtggtaaa tcttggcttt
    19381 gccgcgaaat atgctttctc caaatacgac gatgccgcac accgccatta aaacagcgga
    19441 aagcagggcg gcggtattgt tgttgtagga catttcgtat tcttggaaaa tggcggtggt
    19501 aaaagtgggg tagttcaaaa tagataccgc gccaaattcg accagcatat gcagggcaat
    19561 cagtaacacg ctgctgccga tggcgggttt gagctggggg aggatggcgg aaaaaaaggt
    19621 ttgcaggcgg cttttgccca aggacaggct gacttcttcg taagacaggc tgatgcgttt
    19681 gagtgccgcc tcgacgggca ggtaggcgag cgggaacgag gacaggctca taatcatcac
    19741 tgtcccccaa aagccttcga cacggaaggt caggctgatc caggtgaaac agctgacaaa
    19801 tgcggggatg cacaaaggca gggtgattgc cgtctgaaaa aaggttttgc cgaagaagcg
    19861 gtaacgttgg aacaaaaggg cgcaggcaat gcccaaaaca atggaaatca gggtaacgcc
    19921 cgccatcatc gtcaaggtgt tggagagcaa atcccacata cgcgggcgga acaacagttc
    19981 gacggcgcgg ttgatgccga cctgccacga acgcatagcg acatataaaa aaggcagggt
    20041 aagcggtagg gcaatcagta ggatgaggcc ggtaagccaa atgggtattt ttttaggaga
    20101 catagtgttt tttatcggca aaacgggcgg acagtataaa tgtccacccg tttgacaatc
    20161 cgaaaacggc ttatttcata ccggcttgct caagcagccg ggtggcgtgt tctttttcgg
    20221 aaacagtggt ggcggacact tggggtgctt ccaacttggc gatgggttcc aaattgaagg
    20281 tggataccac gtgcggattc aaaggatact cggcacggac ggcggtcagg gcacgctgtc
    20341 cttccttgcc ggcgaggaag gcgacgaatt ttttcgcctc atccttgttt tgggaggatt
    20401 ttaacacggc tgcaccggaa taggtaatga gtgcgccggg atctctgtgg cggacgaaat
    20461 tcaggcgggt gtggacgttt tgtacgcctt tttcacgcgc gaaagcgtgc cagtagtagt
    20521 tgttgatgag ggcggcatcg atttcgccgt tttcaaccgc ttgaagggcg acggagtttt
    20581 tagcgtaagg cttgccgtat tctttcagac ctttgagcca tttcaatgcg gccgcttcgc
    20641 ctttcagttt gacgatggcg acaacctgtt ccaagaacgc gccggaagtg ggggcgtaac
    20701 cgatgcggtt tttccatttc ggcgtggcgt aattcaggac ggatttttct aaatcttttt
    20761 cagacagttt gcgggtgtcg taaacgacga cgcgcgaacg tccgctcagt gccacccagt
    20821 cttttttggc ggcaaccggc acgcccttgc cgcgtgtttc gttgatggtg gaggcgggca
    20881 ggggctctag gaggttggct gcggaaaggg tggcgagtgc cgggatttgt tcggaataga
    20941 atacgtcggc ggggcttcgg ctgccttctt ctttgatttg accggcaagc tggtcgcctt
    21001 tggcgctgtt gagtttgact ttgatgccgg tagcccgggt aaaggcatct gcaacggctt
    21061 gtgctgcttc tttgtgttgg ccgttgtaca cggtaatgtc tgccagcgcg ggggttgcgg
    21121 cggtcagggc tgcggcaagc agtgcgtatc ggatagatgt tttcatatcg attttctcct
    21181 aatgaatgag agtgtacacc ttgttaaaac ataacggtgt gtagtgtatt ccttcttctt
    21241 tataaatgca aataattatt ttttaaattt gttattatcc gatccggtta ttgtttgttc
    21301 tgacttgtat tttttccgtg catcgcgccc gtaaggcgga agcggcgggc aatgcgtggc
    21361 ggaatgtggg taaaggcggc attttgattt gtcggaatgc ttgagaaccc ctctctttaa
    21421 aacagcgtct gaacagggtt gccggaatag tattgccatc ccggcagata cagtttgtcg
    21481 gggtcttgcc aatattgttc atccagactg ttcggcagtg aggcggtttt gttgtcgaga
    21541 tgttttgtta tatcccacat ttctttcagg tttttacctt ccgattggag gtggcggatt
    21601 tcttttctca atgcgtctgc cgcccgatgg atcagcattg cgctgtgtat cgcgccgatt
    21661 tttttcagaa cggaacaagt cttttcggcg gcgggaaaac cccaattgca gacaaattgc
    21721 agtatgccgc cgttaccgat atcggcttct gtccgccaaa tcaaaaccag ctcctgttcc
    21781 cgcccgtcca tgctttcgag tttgccgtca tgccgttcaa aaagtttgtc caccgcctgc
    21841 cacatcatct gctcgaagcg gtcggcttgg gtatccgtat cggtcatcgt gttcttgcct
    21901 tttaaaaatg ccgtctgaac atttcttcag acggcatttg ggggttaagc caacatttcc
    21961 cgccagcgtt tgacttggaa acggacttgt tcaggcgcgg tgccgcccaa gtggttgcgc
    22021 gcgtttaagc tgccttcggg tgtcagcacg ccgtaaacgt cgtcggcaat caaatcgctg
    22081 aaaccttgta agacttcgag cggcagttcg ctcaaatcga cgccagcttc atcggcgtgg
    22141 cgcacggctt gggcgacgac ttcgtgggca tcgcggaagg gcataccttt tttaaccaga
    22201 taatccgcca agtcggtggc ggtggcaaaa ccctgcatca cggcggcgcg catattgtcg
    22261 ggtttgacgg ttacgccgcg catcatatcg gcgtaaatcc gcaacgtgtc gataagcgtg
    22321 tcggcggtgt cgaacaaggg ttctttgtct tcctgattgt ctttgttgta cgccaagggc
    22381 tgcgatttca tcagggtaat caagccgata aggtgtccga tgactcggcc ggatttgccg
    22441 cgcacgagtt cgggcacgtc ggggtttttc ttctgcggca tgatggacga acctgtgcag
    22501 aaacggtcgg cgatgtcgat aaagccgaaa cgcggactca tccacaaaat caattcttca
    22561 gacaggcggc tcaggtgaac cataaccagc gaggcggcgg cggtgaactc gatggcaaaa
    22621 tcgcggtcgg atacggcatc gagcgagttt tggcagattt gttcaaagcc caacagtttg
    22681 gcggtgattt cgcgccgaat cggataagtc gtaccggcaa gggcggctgc gccgagcggc
    22741 atacggttga cgcggcggcg gcagtccgcc atccgttcgt tatcgcgtcc gagcatttcg
    22801 acgtaggcga gcatatggtg tccgaagctg acgggctggg cgacttgcag gtgggtaaag
    22861 cctggcatga cggtttcggc gttttgttcc gccaaatcca gcaatgccgt ctgaaggctt
    22921 tgaatcaggc ttcggatgat ggtaatctgg tcgcgcagcc aaaggcggat gtcggtggcg
    22981 acttggtcgt tgcggctgcg gccggtgtgc aggcgtttgc ccgcgtcgcc gattttgtcg
    23041 gtcaggcggc gttcgatgtt catgtggaca tcttccaaat cggacgacca ttcgattttg
    23101 ccgctgcgga tttcttcgag gatttccgcc atgccccgac ggatgtccgc caaatcgcct
    23161 tcgtccaaca cgccggtttc tttcaacatt tgcgcgtgtg ccagcgagcc ttgaatgtcc
    23221 cattcggcaa gccgtcggtc gaaaccgatg gaggcggtgt attgtttgac gagttcggaa
    23281 acgggttcgt tgaaacgtcc ggaccaggtt ttgtcgtgca taaggattcc ttgatggggt
    23341 tattcggtgc ggtatttttc caaaaggcgg cggaagggtt cgccggtttc ggggtgtttc
    23401 aggccgtagg cgacggtggc ttcgaggtag cccagtttgc tgccgcagtc gtagcgcgtg
    23461 ccttcaaagg ggtgcgccag gacaaattcg tgatcgagca gcttggcgat gccgtctgta
    23521 agctggattt cgttgcccgc gccgcgcggc agtccggtca gtaagtcgaa aatgcgcggg
    23581 gtaaggatgt agcgtccgac gacggcaagg ttggagggcg cgtcttcggg cttgggtttt
    23641 tcgacaatgc cgataatgcg ttggaactgt ttgagctgtt cggtttcgac gatgccgtat
    23701 gagccggttt gcgatgcttc aacggtttct acgcccaaaa tgctgttgcc gctgcgcccg
    23761 tacacttcga ccatttgttt gagcgcgcct ttgggggcat caatcaggtc gtcggcaagg
    23821 ataacggcaa agggttcgtc tccgatggcg gcgcgggcgc acaagacggc gtgtcccaag
    23881 cccagtgctt ccgcctgacg gatgtagagg caggtaatgt tcggcggcag gatgttgcgg
    23941 acgtgttcca acaatttgtc tttatggcgc atttccaact cggtttcgag ttcgtatgcc
    24001 ttgtcgaaat ggtcttcgat gctgcgtttg ttgcgtccgg taacaaacac catttccgtg
    24061 cagccggctt ccacggcttc ttctacggcg tattggatca gcggcttgtc gacgatgggc
    24121 agcatttctt tcgggcttgc cttggtggcg ggcaggaagc gtgttcccat ccctgcgacg
    24181 gggaaaacgg ctttccgtat gggtttcatt ctttttcctt tgtattgttt tgatgtttaa
    24241 agggcgagtt tgcgtaagag ttcggcaagt gcctgcgcgc ggtggctttc gcggtttttg
    24301 acctccgaat ccaattcggc ggcggttttg ccgtgtccgg gcagataaaa atacgggtcg
    24361 taaccgaaac cgttttcacc ggcgggaacg ccgttccatt gtccgtgcca tacgccttcg
    24421 gcaataatcg gacgcgggtc gtctttatgg cggacaaaaa ccaatacgca gacgtagcaa
    24481 cagcttttgt cggctttgcc ggcaagttcg gcggcaagtt tcaggttgtt ggcggtatcg
    24541 gatttgggat tgctgccggc gtaacgtgcg gaatgtatgc ccggtgcgcc gtttagggcg
    24601 gcggcacaga tgccgctgtc gtcggcgagt gcgggcagcc cgctgtgtcc ggcggcgtgc
    24661 cgggcttttg ccagcgcatt ttcaacaaag gtggaatagg gttcggggca ttcgggtatg
    24721 ctgaactcgg attgcggcaa tacggtaatg ccgtatggtt cgaataggtt gccgaattct
    24781 ttgagtttgc ctgcattgcc gcttgccaaa acgatttttt ccggtttttc agacatagcg
    24841 gttttccttt gcggcggatt gggcggcgcg tagggatttg tgccgcaggt agagggcgag
    24901 gctgccgatt tgtccgaaca gtgcgccgaa tgcgaaaaga aaggaggcga cggcgaaggc
    24961 tttgctgccg acggtcagca gataagcacc caagaggacg agcagcataa atgccagtgt
    25021 gaagagggca acagacaaca gatagatttt tcggcggttc atggcgttcg gtcggaaacg
    25081 gtatgttcgg attatagccg attgggacgg tattccctag agcttggaaa aatgccgtct
    25141 gaaacggcgt tcagacggca tggcgggcgt tatgcctgtt tgttccaacg ttcgatggat
    25201 tctttgatga cttttttcgc ttcttccgca tcgccccacg attcgacttt ggtcgtacct
    25261 gcttttttca ggtctttgta atggttgaag tggaactcga tttgtttgat gagctgttgc
    25321 ggcaaatcgg acaaagtttt gtaggcgttg ccgttgttgc ggtcgtcggc aggaacgcag
    25381 acgattttgt cgtctacttc gccgtcgtca acgaatttca tcacgccgat aacgcgcgct
    25441 tccaagaata cgccggttgc caaaggttgt tcggtaacga gcagcacgtc caattcgtcg
    25501 ccgtcttcgt ccaaagtttg gggaatgaag ccgtagttgg tcggtttggc gaagatggcg
    25561 ggttcgacgc ggtcgagttg gaatgcggcg agtttgcggt tccactcgat tttgtggttg
    25621 ctgccggcgg ggatttcgtt gacgacgttg atgatgccgc cgtccacatc gccgggggtc
    25681 aggatttggt tgaagtctgc catttggttt cctttgtttg ggaaagtttg aagtttgaaa
    25741 gtatagcaca aacgtccggc tgaaaatgcg cccgatgccg tctgaaaggg tgtacgggcg
    25801 cgtgttaccg tttgcccaaa aacctgccca gttccagaat cgcgcgcctg ttggacgggg
    25861 aaaacacttt ttccgccgct tcttccaagc cgaaccagcc gtaggagacg tgttcctcgg
    25921 gttgcaggac gacgggcgta tcgcgcggga tttcggcaga gaagaggtgt tcgcggtttt
    25981 caaacacgcc ttttggatag cggtgccgcc agtggtggta gatttcgtaa accgtgctgt
    26041 cgtgccagtc ttgaagctgc ccgtccgcca gcaggatgcc ggtttcttcc caaacttcgc
    26101 gccttgccgt ttgggcgacg gtttcgcccg gttcgaggct gccggttacc gactgccaaa
    26161 atccttccgg atgcgtgcgt tcgatgagca ggatgccgcc gtccccgcta taaaggacga
    26221 ccagtgcgga aacggggtat ttgagcggtt ttgccatcgg catctttcgg cgggctgcgg
    26281 taatgaaggg gcggattata gcaaacgccg cacgttatgg cgtttatcct tttccgtatc
    26341 cttttttctg cacggatggg acgcgccggt gtttgccggt aaattttccg ttgtgtcaaa
    26401 aagataaggg cggttgtgat tttaatgctt gccaaagcgt cgggcggaaa ctataatccg
    26461 aaacttatcg agtcggagtg tggcgcagtc tggtagcgca cttgcatggg gtgcaagggg
    26521 tcgaaggttc gaatcctttc actccgacca aaaattccga aagccgcttt caaaagcggc
    26581 ttttttgccg tccgtatgat tatgatgtag agtacgcggc gacagacatt caaatgccgt
    26641 ctgaaaaccg ttcagatggc atctctttat cttagtttca ttccgtacca tcttaaggaa
    26701 catcaaattg ggcatttccc gcaaaatatc ccttattctg tccatactgg cagtgtgcct
    26761 gccgatgcat gcacacgcct cagatttggc aaacgattct tttatccggc aggttctcga
    26821 ccgtcagcat ttcgaacccg acgggaaata ccacctattc ggcagcaggg gggaacttgc
    26881 cgagcgcagc ggtcatatcg gattgggaaa catacaaagc catcagttgg gcaacctgat
    26941 gattcaacag gcggccatta aaggaaatat cggctacatt gtccgctttt ccgatcacgg
    27001 gcacgaagtc cattccccct tcgacaacca tgcctcacat tccgattctg atgaagccgg
    27061 tagtcccgtt gacggattca gcctttaccg catccattgg gacggatacg aacaccatcc
    27121 cgccgacggc tatgacgggc cacagggcgg cggctatccc gctcccaaag gcgcgaggga
    27181 tatatacagc tacgacataa aaggcgttgc ccaaaatatc cgcctcaacc tgaccgacaa
    27241 ccgcagcacc ggacaacggc ttgtcgaccg tttccacaat gccggtagta tgctgacgca
    27301 aggagtaggc gacggattca aacgcgccac ccgatacagc cccgagctgg acagatcggg
    27361 caatgccgcc gaagccttca acggcactgc agatatcgtt aaaaacatca tcggcgcggc
    27421 aggagaaatt gtcggcgcag gcgatgccgt gcagggcata agcgaaggct caaacattgc
    27481 tgtcatgcac ggcttgggtc tgctttccac cgaaaacaag atggcgcgca tcaacgattt
    27541 ggcagatatg gcgcaactca aagactatgc cgcagcagcc atccgcgatt gggcagtcca
    27601 aaaccccaat gccgcacaag gcatagaagc cgtcagcaat atctttacgg cagtcatccc
    27661 cgtcaaaggg attggggctg ttcggggaaa atacggcttg ggcggcatca cggcacatcc
    27721 tgtcaagcgg tcgcagatgg gcgcgatcgc attgccgaaa gggaaatccg ccgtcagcaa
    27781 caattttgcc gatgcggcat acgccaaata cccgtcccct taccattccc gaaatatccg
    27841 ttcaaacttg gagcagcgtt acggcaaaga aaacatcacc tcctcaaccg tgccgccgtc
    27901 aaacggcaaa aatgtcaaac tggcagacca acgccacccg aagacaggcg taccgtttga
    27961 cggtaaaggg tttccgaatt ttgagaagca cgtgaaatat gatacgaagc tcgatattca
    28021 agaattatcg gggggcggta tacctaaggc taagcctgtg tttgatgcga aaccgagatg
    28081 ggaggttgat aggaagctta ataaattgac aactcgtgag caggtcgaga aaaatgttca
    28141 ggaaataagg aacggtaata aaaacagtaa ctttagccaa catgctcaac tagagaggga
    28201 aattaataaa ctaaaatctg ccgatgaaat taattttgca gatggaatgg gaaaatttac
    28261 cgatagcatg aatgacaagg cttttagtag gcttgtgaaa tcagttaaag agaatggctt
    28321 cacaaatcca gttgtggagt acgttgaaat aaatggaaaa gcatatatcg taagaggaaa
    28381 taatagggtt tttgctgcag aataccttgg caggatacat gaattaaaat ttaaaaaagt
    28441 tgactttcct gttcctaata ctagttggaa aaatcctact gatgtcttga atgaatcagg
    28501 taatgttaag agacctcgtt ataggagtaa ataaaaatgg caatttggaa ttatcggtac
    28561 taccttatcc catcagctgc tatcagaaat aaatttaatg caaataaaga tatcatactt
    28621 gatgagtatc gatctaatgg ttttcagaat tttaatgaga ataaaagttt tgaaaattac
    28681 tttatcgata atgatgttat attattatca ataataaatg aagcaaaaaa acagcttaaa
    28741 ttaaaagagt cttgggataa agacgcaatc atgttttgtg ataattttgg taatagtctt
    28801 accgtttggc cagatgatat agagtgcgaa cttgatttaa gatttgatta tactaaattt
    28861 attcagaaaa ccattgattg ggcaataaaa tataattgtc tacttgtaat agaaaaaaca
    28921 ggaaatgtag tttcccctaa tataaataat ctgatgtatg aaataaaagc atatttggaa
    28981 agcaagccgt ggcccatatg aaacctaaac tcaacaagta ggatgtgtgc ggaacgcacg
    29041 tatgcggttc tcaaggtttg agctaagagg ccgtctgaaa acagaaaaac tgtttcagac
    29101 gacctttctt ttaaccagtt gccacagcaa ccggacaaaa gcagcctacc tccacatcca
    29161 tataggcaat acaggggaga tattttgtaa attctacgaa tattttacct gctaaacagg
    29221 gtaggatatg gtatgaagcg aacattggct taataaacac tatgtcaaga tcgaatcagg
    29281 ctggtactag attgttgtat tccaatgatg gattgctata tataacaact gatcattata
    29341 tctctgcaac acggtttgga gcttggaaat gggaatataa cttatgcaat tagagattat
    29401 cggtagtaaa atttatacgg aacaagattt tcataatcaa atttcaaaaa tattttctat
    29461 acaagattat tatgggaaca atcttgatgc tctgtgggat ttattaagca caaatgtaga
    29521 acgaccgatt actttggtat ggaaagatgc tatgttctca aaaaataaat tagaaaatat
    29581 atttattgaa atcgtaaatg ttctagaaag agttaagaaa caagatgagg attatggatt
    29641 cgaagaaaaa tttaattata ttttagagta agtaaaccct aattacatta cgtacacagg
    29701 cttaaaactc cccagagcca attaagcaag ccgtaaccca tataaaactt aaactcaaca
    29761 agtagcatgt gtgcggaacg tacgcatgcg gttcttaaag tttgagctaa gaggccgtct
    29821 aaaaacagaa aaaccgtttc agacggtctt tgtttaacgc caccgatcca gcgggttaca
    29881 aagcgcagtc aatgccgctg cgccttatgc ctccgaagca ataggcagaa catttggaca
    29941 cggtgaaaac aaaaacgaaa ccgcccaagc cgtcggacat ttccttttag gagcagctat
    30001 tgcccgcgtc aacggtggta attttgctgc cggcggctcg gcagcagttg cagctgaaaa
    30061 ggcggcggaa catcttgccc aacagtataa cgacggtaaa accgcaatcg atccgcaaac
    30121 aggcgagttc aatgccaacc tgctgccgga acatatcaaa gaggaaatca aatcaaagag
    30181 cggggtgatt gcatcgctga cgggcgcggc cgtgggcggc acgccggtag atgcgcaaac
    30241 cggaggtgcg gtcggacaga atgcggtgga aaacaacctc tatctgacat cggaagcctt
    30301 aaagaaggac aagcagacag ctcgtaaaat ttattccgtc ataaaagagc aagtcaagca
    30361 tgaatgcagt tccacaggaa gaattaccga atgtcgtcaa aatataggac gcattatcga
    30421 atttacccaa gacaaacgct ttgacagtag gtttaaggac ttaaaaaaag aatccttata
    30481 ttacctaaat aaacatcctg atttagtagc ctcttatttg aaggctgaat acgaaaagct
    30541 ggatagggaa gacaaaagta tcctgcaccg ctacatctca cccggggctg aaatcgtttc
    30601 gggcagtttg ggggttgttc tttcaggagt agccggaggc ggatcttgtg ccgagacttt
    30661 cggcttaggc tgtgccgccg ctttggttgg tgtaacgtct tcctacgatc atgtcattac
    30721 tgggacgaaa aacttcggaa aaaaagccag cgagcaacga ccgacgattg cggttcaggc
    30781 cttgaagcag ttggggctgt cggagcaggc tgcggaatat gttcagttct ctatagattt
    30841 gttcagtgtg ggtaaatcgg ggggcggtat acctaaggct aagcctgtgt ttgatgc
//
LOCUS       DAXIEB010000011        27995 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000011 DAXIEB010000000
VERSION     DAXIEB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.011"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFOA3_000432"
     CDS             complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFOA3_000432"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6897491.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGYKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1907..2344)
                     /locus_tag="ACFOA3_000433"
     CDS             complement(1907..2344)
                     /locus_tag="ACFOA3_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897492.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFOA3_000434"
     CDS             complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFOA3_000434"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6897493.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSVIIEVETVGSAAATPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFOA3_000435"
     CDS             complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFOA3_000435"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6897494.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7552..9264
                     /locus_tag="ACFOA3_000436"
     CDS             7552..9264
                     /locus_tag="ACFOA3_000436"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6897495.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEVNSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            9327..9983
                     /locus_tag="ACFOA3_000437"
     CDS             9327..9983
                     /locus_tag="ACFOA3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6897496.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGQDADGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFOA3_000438"
     CDS             complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFOA3_000438"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6897497.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTEHEMTHLSRKFGRRLHGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGRKG"
     gene            complement(10441..10989)
                     /locus_tag="ACFOA3_000439"
     CDS             complement(10441..10989)
                     /locus_tag="ACFOA3_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6897498.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPVGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10991..11551)
                     /locus_tag="ACFOA3_000440"
     CDS             complement(10991..11551)
                     /locus_tag="ACFOA3_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6897499.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11879..13684
                     /locus_tag="ACFOA3_000441"
     CDS             11879..13684
                     /locus_tag="ACFOA3_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6897500.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13811..15295
                     /locus_tag="ACFOA3_000442"
     CDS             13811..15295
                     /locus_tag="ACFOA3_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6897501.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15552..17651
                     /gene="uvrB"
                     /locus_tag="ACFOA3_000443"
     CDS             15552..17651
                     /gene="uvrB"
                     /locus_tag="ACFOA3_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6897502.1"
                     /translation="MPSERRFRPIPTTRFVRKRITGKIMEVIRYPDSPFKLHQPFPPA
                     GDQPTAIADLLEGLSDGLAYQTLLGVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQ
                     LYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNL
                     MTRDDVIIVATVSAIYGIGDPTEYQQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDF
                     KRGSFRVRGDVIDVYPAESSENALRISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPS
                     SHYVTPRDTVLRACESIKEELRERIEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCK
                     GIENYSRHFSGKKEGEPPPTLMDYLPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLV
                     DYGFRLPSARDNRPLKFHEFEKVMPQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDP
                     QIIIRPVATQVDDLMSEINDRIQKGERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSD
                     IDTVERVEIIRDLRLGLFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQ
                     TIGRAARNVNGVAILYADKITDSMKAAIDETERRREKQIKFNEEHGIVPQQIKKQVKD
                     IIDGVYHEEDSGKGRRQGKNKVKVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEASV
                     LRDRIRGIREGLLFGSE"
     gene            17780..18067
                     /locus_tag="ACFOA3_000444"
     CDS             17780..18067
                     /locus_tag="ACFOA3_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897503.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIEVVYVLEQQQG"
     gene            complement(18248..18964)
                     /gene="trmB"
                     /locus_tag="ACFOA3_000445"
     CDS             complement(18248..18964)
                     /gene="trmB"
                     /locus_tag="ACFOA3_000445"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6897504.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(19086..20489)
                     /locus_tag="ACFOA3_000446"
     CDS             complement(19086..20489)
                     /locus_tag="ACFOA3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6897505.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(20607..22493)
                     /gene="uvrC"
                     /locus_tag="ACFOA3_000447"
     CDS             complement(20607..22493)
                     /gene="uvrC"
                     /locus_tag="ACFOA3_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6897506.1"
                     /translation="MPSESVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQAAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(22549..24711)
                     /locus_tag="ACFOA3_000448"
     CDS             complement(22549..24711)
                     /locus_tag="ACFOA3_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6897507.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPTAQNAQTVVGAGIAAEVKGAGLVKAGKAEF
                     AELTLPKFSDGVWEIASVVAVSVNGKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            24850..25800
                     /gene="trxB"
                     /locus_tag="ACFOA3_000449"
     CDS             24850..25800
                     /gene="trxB"
                     /locus_tag="ACFOA3_000449"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6897508.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            25956..26324
                     /gene="rpsF"
                     /locus_tag="ACFOA3_000450"
     CDS             25956..26324
                     /gene="rpsF"
                     /locus_tag="ACFOA3_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6897509.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTESSPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            26325..26627
                     /gene="priB"
                     /locus_tag="ACFOA3_000451"
     CDS             26325..26627
                     /gene="priB"
                     /locus_tag="ACFOA3_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6897510.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGDCATVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            26634..26864
                     /gene="rpsR"
                     /locus_tag="ACFOA3_000452"
     CDS             26634..26864
                     /gene="rpsR"
                     /locus_tag="ACFOA3_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6897511.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            26881..27333
                     /gene="rplI"
                     /locus_tag="ACFOA3_000453"
     CDS             26881..27333
                     /gene="rplI"
                     /locus_tag="ACFOA3_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6897512.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPTGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            27347..27454
                     /locus_tag="ACFOA3_000454"
     CDS             27347..27454
                     /locus_tag="ACFOA3_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC6897513.1"
                     /translation="MPSETLPIIGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            complement(27806..>27995)
                     /locus_tag="ACFOA3_000455"
     CDS             complement(27806..>27995)
                     /locus_tag="ACFOA3_000455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897514.1"
                     /translation="VFGNHGLCGLPEEGAHEKSPKCLGGNLGDFGGFCKGLKRYFRHN
                     DRHTSRNLCNHIYKLLNF"
BASE COUNT         6624 a         6972 c         7622 g         6777 t
ORIGIN      
        1 gccggaggaa atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgcag ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcagcatc aaaaatcagc ttggtgaaac cattgtcgca accgttggca
      361 atcgcacggc cggaagctgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgcca
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tagccggcgc agttttcagc ggcaacgtgg
      541 ccttcgtgaa cggctttgtg cgccaacatc ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cgttggtacg catttgtttg tccacttcga tgaagccgcg atcggttacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtagcgttg cggctctttc ggtgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cggatctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca gggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgct cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccag ggccgccgcc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcggca gctttagggg cttcttgcgc cggggcggca
     1621 gccgcttcgg ctttaggggc ggcagccgtg ccttcagctt caacgacaac aatcaaaccg
     1681 ccttcggaga ttttgtcgcc gactttgact ttcacttctt tgaccacgcc cgcaacttcg
     1741 gcaggcacgt ccatcgtcgc tttgtcggtt tccaaggtaa tcagggtatc gtccacagca
     1801 atagtgtcgc ccacgttcac ttcaactgcg ataatatcta cattttcgtg tccgccaatg
     1861 tcgggcactt tcaattcaac taaagccatt atattgacct ttcaacttag ttaatttcta
     1921 tcaactcttt agcaataatc acttcttcag gaatacagcc acaatcttca gctctgattg
     1981 ttacttcctt ctcatttggg ttttctggat gttcaacctc atattcaaaa atagaatatt
     2041 gaccaaacaa atccctattt aaaacatata tatagccatt ttcgatgccg gaacttgttg
     2101 caaatttctt ggcaatttcc ttgtctgttg tcgtagatat ataacatccg tcatatagac
     2161 ctgtttcaat ttgatgggcg taaactgcat tcttcacact tggaccatgt gtagctttac
     2221 catcatattt aaacttacca tcataacgaa ttgcaacttc agctttatta cctttaggtt
     2281 ttaactgtcc attattttgc tcatcttgtt ggcaactaat gcctctatat aagaaatttc
     2341 ccataagtta ttaatttaaa ggttgaattt aacaccatta ctcatcaatc tcaagcaatc
     2401 caaacattat cagattgctt gagagggatg ttttatttta taaggtaatg cggcggaagt
     2461 ctttcaatag cttcgccaag aatacggtga agcgcatacc ggccgcaccg tcgatgacgc
     2521 ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct ttgccgttcc
     2581 aaaccggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga gcgttcacaa
     2641 ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag cacgcgcctt
     2701 gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg gtcaattctt
     2761 ggctgatttg tttcaaacct ttttgatcca cgtctttgat gacgggaaca accaagccgt
     2821 tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg ttgtcgccgt
     2881 ccagagaagc gttgaattcg gggaaggctt tcagcgcgga aacggaggct ttgatgatga
     2941 acgccagagg agacagtttc acgccttcgc gttcccattc tttgttcagt tgtttgcgga
     3001 attcttccag ctcggtcata tctgcttctt cgtgtacggt aacgtgggga atcaccaccc
     3061 agttgcgaga caggttttga ccggagattt tcttaatgcg ggacaattct ttaacttcga
     3121 cattgccgaa tttggagaag tccactttag gccacggcag caagtccaga ccgctgccca
     3181 aagatgcgcc ggctgcggca ggttttgccg cgccaccctg cattacggat ttcacaaagg
     3241 ctttgatgtc gtcgcccacg atgcggcctt tcaagccggt acctttgact tggcccaaat
     3301 ccacgcccaa ttcgcgcgcc agtttgcgtg cggaagggcc ggcgtgtgct ttggcgaaag
     3361 cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg gcgggtgcgg
     3421 cggcagcagt cggaggcgga gcggcagccg gtgcggcagc ttgagcggct tgagcagggg
     3481 ttgctgcggc agagccgacg gtttctactt cgataatgac agagccttcg gatactttgt
     3541 cgccgacttt taagaatacg gctttaacga caccggcagc ggtacaaggt acgtccattg
     3601 tcgctttatc ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt
     3661 tgatttcaac ggcgatgaca tccacatcgg tatggccgcc gatatcggga acggctactt
     3721 gaacggttgc accgcctgcg gcaacgggtg cggcagcagg tgcaggttgt gcttcggcag
     3781 cagccgaggc ggcttcggca gcggcggcac cggtttcaac ggtcaaaatt acgccgcctt
     3841 cggagatttt gtcgccgact ttgactttta cttctttcac gacaccggca gcatcggcag
     3901 gcacatccat cgtggctttg tcggtttcca gtgtaatcag ggtgtcgtca acggcgatgg
     3961 tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg ccgatatcgg
     4021 ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcagt tggatgccgt
     4081 ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt cgggcattcg
     4141 gttttcagac ggcctgatgt cggaatgggg caaacagacc gtctgaaaca tcaatcagcg
     4201 tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt gttgaacggt
     4261 ttctttgctg actttgcctt gttccgccaa tgcgctcaat gctgccacgg caacgttgta
     4321 gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc cgaagccgtc
     4381 agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag catagctgcg
     4441 gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcagttggg aagtaacgaa
     4501 aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct cgacagcgtc
     4561 gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc
     4621 tttcagcagc tcggcaccgg caatcacttc ttgcaaaatc gtaccggagc ccatcaattg
     4681 aactttcttg tcgcctttgc cgccggcttt cagcaggtac atacctttca agatgtcttg
     4741 ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg tgatgtagta
     4801 gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga tgacggcgac
     4861 ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg cctggatgtg
     4921 gctgtggccg tcttcgtgtt gcaggccttc gccgttcagc gtcgtacggc cggcagtacc
     4981 gcccagcagg aagccgcgcg cgtgcatatc gcccgccgcc caagccaagt cgccgatacg
     5041 ttggaaaccg aacatagaat agtaaatgta gaacggaatc atcgcgaagt tgctgttggc
     5101 gtagctggtt gcagccgcaa tccagtcggc catcgcgccc ggttcgttaa taccttcttg
     5161 caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt cttgaggggt
     5221 atattgctga cctttcggat tccaaatacc gtattggcgg aacatacctt ccatgccgaa
     5281 agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt tgtctttcag
     5341 caaagtggac aggatgcgga cgaatgccat cgtggttgag aactcgcgtt caccgctgga
     5401 tttgagttgt gcgtcgaatg ctgacagctc gggcacttcc aatacttcct gcgtcggttt
     5461 gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc
     5521 cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac
     5581 cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt ttttggcttg
     5641 gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa tggttttcgc
     5701 caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac
     5761 tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc
     5821 aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt
     5881 ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc gcaggatacc
     5941 gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt tgacgacatt
     6001 ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt tgccgcgtac
     6061 cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt ccaagccttc
     6121 gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc cgtcgccgca
     6181 gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca agtatttcag
     6241 gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg tcgggaactg
     6301 ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc
     6361 ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata
     6421 gatgcccggg gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt
     6481 ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt ggaaagatgc
     6541 gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg cggcggcgtt
     6601 ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc
     6661 gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc
     6721 cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgtgcgcgtt cgccgccttc
     6781 atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag ggtcaacatc
     6841 gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg gcagggaggg
     6901 tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga aaatttctaa
     6961 atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa
     7021 acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg
     7081 ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg
     7141 taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga
     7201 attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat
     7261 acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg
     7321 caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg
     7381 tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttgtcc
     7441 cgcgcttggg gcagggggta aattttggag gttggtgcaa aacctgcttt tttcgcgtta
     7501 caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatatttt tatgaaagcc
     7561 agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac
     7621 aagctgatga tacgcgccgg tctgattaaa gccaacgcgt ccggccttta tacttggatg
     7681 ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc
     7741 gcgggcagcg tggagctgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc
     7801 ggccgctggg aattttacgg taaagaactg ctgcgcctga aagaccgcca cgaccgcgat
     7861 ttctgcatgg gtccgacttg tgaagaagtg attgcggaca tcgtgcgtaa agaagtcaac
     7921 agctacaaac aattgccgaa aaacttttac cacatccaaa ccaaattccg cgacgaagtg
     7981 cgcccgcgct tcggtgtgat gcgtgcgcgc gagtttgtca tgaaagatgc ttattccttc
     8041 catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc ttactgccgt
     8101 atcttcaccc gtctgggctt ggcattccgc cccgttgccg cagataccgg cagcatcggc
     8161 ggtaccggct ctcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac
     8221 agcgacactt ccgattacgc cgccaatgtc gagctggcac cgaccttgcc gcttaaaggc
     8281 gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaatgt caaaaccatt
     8341 gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtc
     8401 gaaagcgaaa acgaaggcga aatcgtctta ttactgttgc gcggcgacca tgagttcaac
     8461 gacatcaaag ccgaaaaact cgcgggcgtg aaatcgccat tgacgatggc aagtcctgcc
     8521 gcgattgtcg aacagttcgg cgcaaacggc ggctcgctcg gccctgtcgg cttcacaggc
     8581 aaagtctatg ccgatttcgc taccgaaaaa ggcgcggact gggttatcgg cgcaaacgaa
     8641 gacgactacc actataccgg gttcaatttc ggccgagacg ctgccgagcc tgagtttgtc
     8701 gatttgcgca acgtcgtcga aggcgacgaa agccccgacg ggcaaggccg tctgaaactg
     8761 gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgtg acaaatatac taaagcgatg
     8821 aatgtcagct tcttggacaa caacggcaaa tcgcaaatta tggaaatggg ctgctacggc
     8881 atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga aaaaggcatc
     8941 atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa
     9001 tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg
     9061 gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaacga ttccgagctt
     9121 ctcggtattc cgcaccgcat cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa
     9181 tacgccgaac gccgcgacaa cgaagcgcag gcaattgcaa tcggagaaat tgttgcacgt
     9241 gtaacagctt cattaaatgc gtaagcttgg tcgaatgccg tctgaaaata atttcagacg
     9301 gcatttttac ttagggggaa agtgaagtgg cttcagtatt tttagaagga gaggcagtcg
     9361 aggtcggcaa tattttctgt atcggcagga actatgccgc acatatcgaa gagttaaaaa
     9421 acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaatagcg
     9481 gcggtacaat cttgctgccc gagtttagta gggatgtgca gtttgagtgc gaactcgtgt
     9541 tgctggtcgg tcaggatgcg gacggcatgg gcgagggtga agatattttg ggatgcgtgg
     9601 cggggtacgg cgtgggtttg gatttgaccg cgcgggacat ccaatgccat ctgaaggcgc
     9661 aggggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg
     9721 cggcggcaga cagaatcggc aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg
     9781 tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca
     9841 aactggcggc ggattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg
     9901 gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcgcg
     9961 cctctttcac ggtagggtgt taaccgcgcc gcttaaccct tacgcccgtt gaaacattcc
    10021 gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc
    10081 gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggat
    10141 gacagccgtt cgtccaccca atagacgggg agattgaacc tgccgtgcag cctgcgcccg
    10201 aacttgcggg acagatgcgt catttcgtgt tccgtgccgt cggtatgcac gggcagtccg
    10261 acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca
    10321 tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct
    10381 tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt
    10441 ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt tggcaaaggc
    10501 ggcggcgtaa cggtgttcgt aggggatgtc gaacaagata tgttcgtcgg cgggaacagt
    10561 cagccacgca ttgtcggcaa gttcgcgttc gagctggcct tttccccagc ttgaatagcc
    10621 tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atgttttcaa gcacatcgcg
    10681 ggaagaggtc agcgcgatgc cgtctgaaac gccgatgctg ctttgccagt tgccgaccgg
    10741 cgtgtggacg acgtaaccgc gctcgacctg caccggcccg cccatcatca cgctgtcgtg
    10801 ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg
    10861 agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac
    10921 gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgacgagaa agtggttgga
    10981 gaggttcata tcagcgtttg tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg
    11041 caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga
    11101 aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca
    11161 ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga
    11221 acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa
    11281 aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca
    11341 aattgaccat ttccttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt
    11401 gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc agcatgttga
    11461 aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga
    11521 cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt
    11581 tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc
    11641 aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg
    11701 cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    11761 gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    11821 cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat
    11881 gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc
    11941 ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga
    12001 acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa
    12061 aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa
    12121 ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg
    12181 ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa
    12241 agaaaaaaac gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca
    12301 gggcaatccg cgtaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt
    12361 ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa
    12421 tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga
    12481 aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct
    12541 caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca
    12601 ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaagttcca
    12661 aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat
    12721 ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg ccctgttcct
    12781 gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc
    12841 ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga
    12901 gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatctct
    12961 gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc
    13021 caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc agcagctgaa
    13081 caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc
    13141 aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa aagaagcggc
    13201 gcagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa
    13261 catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg
    13321 acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga accggagcgg
    13381 cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg aaagcattgc
    13441 gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga
    13501 tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg tcggcaaggg
    13561 ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga
    13621 ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga gctggatacg
    13681 ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc
    13741 cgtgaagttt gccgtttgcc gtacctgtat tttccggttt ttggaaatca agagagaaag
    13801 ttaaagaaga atgtcgaaac ctgtttttaa gaaaatcgca ctttatactt tgggtgcaat
    13861 cagcggcgtg gccgtcagtc tggcggtgca gggttttgcc gccgagaagg acaggcggga
    13921 taacgaagtc ctgccggtgc aatccatccg cacaatggcg gaggtttacg gtcaaatcaa
    13981 ggcaaactac tatcaggaca aacccgatgc cgatttgttt gaaggtgcga tgaagggtat
    14041 ggtggccggt ttggatccgc attccgaata tatggataaa aaaggttatg ccgagataaa
    14101 ggagtccacc agcggcgaat ttggcggctt ggggatggaa atcgggcagg aagacggatt
    14161 tgtcaaagtg gtttcgccga ttgaggacac gcctgcggaa cgggcggggg tgaaaagcgg
    14221 cgatttcatt gtgaaaatcg ataatgtttc gacacgcggc atgacggtca gcgaagcggt
    14281 gaagaaaatg cggggcaagc cgggtacgaa gattactttg acgctgtcgc gcaaaaatgc
    14341 cgacaagccg atagtcgtca acctgacccg tgccattatt aaagtgaaaa gcgtccgcca
    14401 tcacctgatc gaacccgatt acggctatat ccgcgtgtcg cagttccaag agcggacggt
    14461 cgaaagcgtc aataccgccg caaaagagct ggtaaaggaa aataaaggaa aaccgctcaa
    14521 ggggctggtg ttggatttgc gcgacgaccc cggcgggctt ttgactggcg cggtcggcgt
    14581 gtcggcggca tttctgccgt ctgaagcagt cgtcgtcagc accaagggac gcgacggcaa
    14641 agaccgcatg gtactgaaag ccattcctga agattatgtg tacgggatgg gcggcgattc
    14701 gttggcgggc attcctgccg agttgaaaac cataccgatg acggtattgg tcaattccgg
    14761 ttcggcttcc gcgtcggaga ttgtcgcagg tgcattgcag gatcataaac gcgcggtcat
    14821 cgtcggtacg cagagcttcg gcaaaggttc ggttcagact ttgattcctt tgtccaacgg
    14881 cagcgcggtc aagctgacaa cggcactgta ttatacgccg aacgaccgtt ctattcaggc
    14941 gcaggggatt gttcccgatg tcgaagtaaa agataaggaa cgcatttttg aaagccgcga
    15001 ggcggatttg gtcggacaca tcggcaatcc tttgggcggc gaggatgtga acagtgaaac
    15061 ccttgccgtg ccgcttgaaa aagatgcgga taagcccgct gtaaaagaaa aaggtaaaaa
    15121 gaaaaaggac gaggatttgt cttcaaggcg gattcccaac cctgccaaag acgaccagtt
    15181 gcggaaagct ttggatttgg tcaagtcgcc cgagcagtgg cagaagtctt tggggctggc
    15241 ggcgaaaaag ccggtttcaa ataaagataa gaaagataaa aaagataaga agtaggtttt
    15301 tacgatgccg tctgaaagga tttcagacgg catattatgt ttgaaaggac aggttatgag
    15361 cgatttttta ccggaatttg aaccgtgcgg catttatagt gggttaacaa aaatcaggac
    15421 aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac gccgtactgg
    15481 tttttgttaa tccactatac ttgggtagcg attgggtttc agacggcata cattcaggat
    15541 ttcaccgcct tatgccgtct gaaagacgtt tccgacccat ccctactaca cgctttgtcc
    15601 gcaaacgcat cacaggcaag attatggaag tcatccgata ccccgattcc cctttcaaac
    15661 tccaccaacc cttcccgccc gcaggcgacc agcctaccgc tattgccgac ttgctcgaag
    15721 ggctttcaga cggccttgcc taccaaaccc tgctcggcgt aaccggttcg ggcaaaacct
    15781 acaccatggc gaacgtcatc gcccaaagcg gccgacccgc catcatcatg gcgcacaaca
    15841 aaacccttgc cgcccagctt tatgccgaaa tgcgcgagtt tttccctgaa aacgcggtgg
    15901 aatatttcgt ctcctactac gactattacc aacccgaagc ctatgtgcca agccgcgatt
    15961 tgttcatcga aaaagacagt gcgatcaacg aacacattga acaaatgcgc ctttctgcca
    16021 ccaaaaacct gatgacgcgc gatgacgtga ttatcgttgc cactgtgtcc gccatttacg
    16081 gtatcggcga cccgaccgag tatcagcaaa tggtgttgtc tgtcaaagaa ggcgacacca
    16141 tcgagcagcg cgacatcatc gccacgctcg tttccatgca gtacgaacgc ggcgatttgg
    16201 acttcaaacg cggcagcttc cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa
    16261 gctccgaaaa cgccttgcgt atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt
    16321 tcgacccgct ttcaggcagc cttattcaac gcgtcggccg ctacaccgtc ttcccgtcca
    16381 gccactacgt taccccgcgc gacaccgtat tgcgcgcctg cgagtccatc aaagaagaat
    16441 tgcgcgaacg catcgaattt ttcgcccgcg aacaacgccc cgtcgagcaa caacgcatcg
    16501 aacagcgcac ccgtttcgac ctcgaaatgc tctacgaaat gggcttctgc aaaggcattg
    16561 aaaattactc ccggcacttc tccggcaaaa aagaaggcga accgccgccc acgctgatgg
    16621 actacctgcc tgacaacgcc atcatgttca tcgacgaaag ccacgttacc gtcacccaaa
    16681 tcggcggcat gtacaaaggc gacgcatcgc gcaagcaaaa cctcgtcgac tacggcttcc
    16741 gcctgccttc cgcccgcgac aaccgcccgc tcaaattcca tgaatttgaa aaagtcatgc
    16801 cgcaaactgt cttcgtttcc gccactccag ccaaatacga agaagaacac gccggacaag
    16861 tggtcgaaca agtcgtccgc cccacagggc tggtcgaccc ccaaatcatc atccgccccg
    16921 tcgccaccca agtcgacgac ttaatgagcg aaatcaacga ccgcatccaa aaaggcgaac
    16981 gcgttctcgt taccaccctc accaaacgca tggcggagca actcaccgac tattacagcg
    17041 aactcggcat caaagtgcgc tacctgcaca gcgacattga taccgtagag cgcgttgaaa
    17101 tcattagaga tttacggctc ggcctgtttg acgtactcgt cggcatcaac ctcttgcgcg
    17161 aaggcctcga catccccgaa gtctccctcg tcgccatcct cgacgccgac aaagaaggct
    17221 tcctgcgctc acaccgcagc ctgattcaaa ccataggccg cgccgcgcgc aacgtgaacg
    17281 gcgtcgcgat tctgtacgcc gacaaaatca ccgactctat gaaagccgcc atcgacgaaa
    17341 ccgaacgccg ccgtgaaaaa caaattaaat tcaacgaaga acacggcatc gtgccgcaac
    17401 agattaaaaa acaggtcaaa gacatcattg acggcgtgta ccacgaagaa gacagcggca
    17461 aaggccgtag acaaggcaaa aacaaagtta aagtcggcga aattcacaac gaagaagacg
    17521 cgattaaaga aatcgccaaa ctggaaaaag ccatgcagca ggcggctagg gatttacagt
    17581 ttgaagaggc gtcggtgtta agggatagga ttcgggggat tagggaaggg ttgttatttg
    17641 ggagtgagta ataaaagcac ttgaaagttt agttgatgac cacaatgttc ttaaaagatt
    17701 tggagaaaca aaagatttgg agaaacaaaa gatgtggaga aacatcgtaa cttgatcaat
    17761 tacattctga ggactgaaaa tgacatattc ttttcgtgta gaaaacataa atgatatatc
    17821 tgatattcct gaaaatcatt tggaaatagt tagagctgct ctgcctagta atagaagtaa
    17881 tgccttatct cgtgagccag atggggcaca agctgttttt gtttatgaaa ttgatggtga
    17941 atttagagct aagttatttg accgcataaa agatacagac agttatttac aagcaacaga
    18001 ggataattac tctaatgacc ctcgcagtat agaagtagta tacgttttgg agcaacagca
    18061 agggtagcct gagggtcgca caaactctca agctgacgtg cagtatgtgt gatacgcacg
    18121 catgcgtttt ttcgttcaaa ccattgtttt aaaccttgta tcgaaacatc actcgctgct
    18181 ttatcttgaa aaaatgccgt ctgtaagcaa acatgcttca gacggcattt tttcaaacag
    18241 cggtttgtta tctgatccgt ttgaatacca agtcccaaac gccgtgtccg agacgtttgc
    18301 cgcgcgcttc gaatttggtt tcggggcggt agtccggggt aggggcgtag tctgtcgccg
    18361 tattttgcag gctgtcgaag ctactgagga cttcaagcat ctgctgtgca tattcttccc
    18421 agtctgtcgc caggtggata tagccgccgg ttttgagttt gggcagtagt ttggcgatga
    18481 agggggcttg tatcagacgg cgtttgtggt ggcgtttttt gtgccacggg tcggggaaga
    18541 atatgtggat gccgtcgagc gagccgtctt gcagcatatt ttcgacaact tctacggcat
    18601 cgtgccgcat cacgcggata ttttccaaat ggttttcgtc tatgagtttg agcaggttgc
    18661 ctacgccggg accgtgtacg tcgatggcga ggaagtcggt ttcgggcagg cggcgggcga
    18721 tttctgcggt tgccgtcccc ataccgaagc ctatttcgag gattttgggt cggctgcttc
    18781 cgaaacgggc atcaagatcg gccggtgttg cttggtagtc gatgccgaag ctgtcccata
    18841 aggtatcgat ggcgcgttgc tgcgcggcgg tcatatggct ttggcggagg acgaagctgc
    18901 ggatgctgcg ggggtgtcct gcttgacggt cgtttggtgt ttggttttcg gcggtgtctg
    18961 tcataatgct tccgtgcgct tgtgcaatca ggatgccgtc tgaatgtgaa aggcctgttc
    19021 ggacggcggg aaaggaaagg ggcgattata cctgtttcgt ttcggcaggg taggtgtaac
    19081 gctgttcaga cggcattttg ccactgttgc aacagttctt gcaggacgct tttttgcccg
    19141 tatccgttgt ttatggcttc ttgcaggtag cggcaggctt tttctttgtc cggcccgaca
    19201 ccgtgtccgc tgtaatggat gcaggcgagg ttgtagaacg ccatactgtc ttttttcgcg
    19261 gcggcttttt caaaccattt gcgtgcttca ttgtagtcgg cggtcatacc ctgtccgaag
    19321 taatacatgc tgcccagatt ggtttgggct ttgctgtgtc cgagttctgc ggcttctgtg
    19381 tagagtttga gcgcggctgc gtattcgggc gcgcattgta atccgtaatg cagggcttgg
    19441 gctttttgat aaagctgttc tgccctttgc cgcctgatgg cggaatcttt aatgccgccg
    19501 tattggttag gatggtttaa cgcgctgtcg gatatgagtt tctgataggc ggaaaggctg
    19561 ccggcttcgg ctgcctgccg ataataatgc cgggcttttt ccgtatcggg ggctatgccc
    19621 aaactgtagc ggcagatgtc gcccagtatc cgcaaacctt cgggatggcg ttcggcggcg
    19681 gctgcttcgg cgtgatgcag ggcaagtttg tggtttcttt ccagatgctt gccttgtagg
    19741 tagatgtcgg caagtgcggc atgggcatcg gggtcatttt cctgtgccgc ctgttggaac
    19801 cggtaaacgg cttcatcggg agccgtaggt aggatgagtt cggcaagcgg ggtgtaggcg
    19861 ggaatgtatc cttgttgtgc ggcggcgcgc aaatgattga tagcttgggt cgtgtcggtt
    19921 ggtgtacctt gtccgaaacg gtattgaagg ccgagctgcc attgtgccgc cgccaagcct
    19981 tgtgctgcag cctcgcggta gagaaggtgt gccgtttcaa aatccggtgg cgtaccgtac
    20041 tggcagtatt gggcaagcag atattgtccg tgggggttgt tgttttttgc ggtcgttttt
    20101 gcccatttca gggcttcttc aaaatgtcgg gacgcataca gaaggtacat caggcggact
    20161 tgtgcttcgg gcagtccttc ctctgccaac ggtctgtaaa gcggtatcag ggtttccggg
    20221 cgcgcgcctt ccaattcttg gatatatgcc agattgtaac gcgcgtaggg atgtccctgt
    20281 tcggcggcga tgcggagcat ttccatacct tgttcgcggt cttggtaggg gctgtccgcg
    20341 tccatcaggt gttttgccaa ctcgaatgcg gcggcgggat gacctgccga ggccgcttct
    20401 gtcagcagtc ggcggacttc ggtaaagtta tattgttttt gagacaggcg ttctaaggcc
    20461 tgccgatagg gtaagtcggg attgttcata tcaggtcgtt ttccgttttg tcgaatcatc
    20521 gttattcgtt atgaagttag gtttatatcg gcacgcctgc ctactgtatc atatttttta
    20581 cagacggttt ttgcttgggc agcatgctaa tgcagatgat tgtaaatcgt ttccgccaat
    20641 gccttgctga tgccttccac tttttccaag tcctcgcggc tggcggcaat cacgccgcgc
    20701 agaccgccga agcgggtaag cagggcttgg cggcgtttgc tgcctacgcc ggggatgtcg
    20761 cttaaggagg aggtaacgcg ggctttgtcg cgttttttgc ggtggccggt aatggcgaaa
    20821 cggtgcgatt catcgcgtac ggtttgcaat agatgcaagg ccgggctgtt gggcggcagg
    20881 cggaagactt cgccagtaaa aggcagtatg agttcttcca taccggcttt gcgctccggg
    20941 cctttggcga taccgaccaa aggaatgtgc agcccgagtt cttcccatac cgatacggct
    21001 acgccgattt gccctttgcc gccgtcaatt aacacggcat ccggccattt gacggtctcg
    21061 ccgttggctt cggcctcctg cattttgccg taacggcgcg tcaacacttc gcgcatggcg
    21121 gcgtagtcgt cgcccggttt ggcggtggtg atgttgtagc ggcggtattg cgagggctgg
    21181 atgttttgct catcgtacac aacgcaggac gcaatagtgg cctcgccttg ggtgtggctg
    21241 atgtcgaaac attcgaggcg gttgaggccg tctgaatcca taccgaggat tttggcgagt
    21301 tcgtcgatgc ggtgttgctg gctgctttgt tgcaggcggc gttgtgcaat cgccatttgt
    21361 gcgttttgct ccgccatttt caaccaaact ttgcgttcgc ctatggtctt ggtgacaaat
    21421 tgcatttgtt tgccgtgttc gctctctagg gcttctttca atgcgtcggg catggggaag
    21481 ttgctgatga tgatgtcagg tttgcttttg cccagatagt gttgggcgac gaaggcttcg
    21541 gcatagtctt gcccatttgg ctcgggatcg tttttggtgt cggggaagaa acttttgtct
    21601 ccgacgtgcc gcccaccgcg tatgctgacc cagtgtacgc agacgatgcc gtctgaaacc
    21661 gccagcgcga gcaaatcgat atcgttgggg ttgttcgggt ttttgctgtc gatgaactga
    21721 ttactctgca taatgccgag tgcttggatt tggtcgcggt aacgggcggc ttcttcaaat
    21781 tgcagattcg cggcggcggc ctgcattttg tgttgcaggg tacgcgtcag ttcgtcggtt
    21841 ttgccattga ggaaagttgc tgcttcacgc acgctgtcgc gataatcttc ttcgctgatg
    21901 tggcctacac aaggcgcggt gcagcgtttg atttggtaaa gcagacaagg acggtcgcgg
    21961 tgctcgaata cactgtcttc gcaggtacgc agcataaaga ctttttgcaa cacttgaata
    22021 ctgtcgcgca cggcgttgct gttgggatac gggccgaaat attgatttgg cttcttcagc
    22081 gtgccgcggt aatacgccat ttgcggatat tgatggccgc tgagcatcaa ataaggatag
    22141 cttttgtcat cgcggaaaag aatattgtat ttcggcgata aggctttgat gaagttgttt
    22201 tcgagaatca gtgcttcggc ttcggaacgc gtgatggtgg tttcgatatg atgcacctgt
    22261 tttaccatca atgcgatgcg cggcgaatgg tcgttttttt ggaaatagcc ggacacgcgc
    22321 cgcttgaggt tgaccgcttt gccgacgtat aagactttgc cgccttcgtc aaaaaaacgg
    22381 tacacgcccg gcagcttggg caggtttttg aggaagagcg ggatgtcgaa ttgttccgga
    22441 gaacggtttt ctgtattcat atcaatcatt tgaaaaacgg attcagacgg catatcgaca
    22501 acggcagcct tatccgttcg gaaaagctgt cgggcgagtt atatgcggtc aatcgatttt
    22561 tacccgtttc aggcgcaagg cgttgctcaa cacggaaacc gagcttgccg ccattgccgc
    22621 gcctgcgatg acggggttta aaaagccgag tgcggcgagt ggaatgccca agatattgta
    22681 gaagaaggcg aaaaacaggt tttgcttgat gttcttcaaa gtcgcctgcg ataccaacag
    22741 tgcatcggcg agctggttga ccgaatgctg catcagcgtg gcggaggcgg tatgttcggc
    22801 aacgtccgca ccgcctttca tggcgaagct gacgttggcg gcggcgagcg cgggtgcgtc
    22861 gttgatgccg tcgccgacca tcgccacggt tttgccggcg gctttgagtt tctgtacttc
    22921 ggcagccttg tcgcgcggac tcatattgcc gaaggcgtgt gcgatgccca gttgtttggc
    22981 gacatattcg accgtgcctt ggttgtcgcc gctcataata tagacatcga tattgtgttt
    23041 tttcagacgg cctatggctt cggcggtatc ggctttcaac gcgtcggcga gtgcgaatgc
    23101 gccgataggt ttgccgttta cagatacggc aaccacactg gcgatttccc aaacgccgtc
    23161 tgaaaacttc ggcaaggtca gttcggcaaa ttcggctttg cctgctttta ccaaacccgc
    23221 gccttttact tcggcggcaa tgcctgcgcc gacaacggtt tgggcgtttt gtgcggtagg
    23281 aatgtccaaa ccgcgcgctt gggcggcgga aacgatggct cgggcgagcg gatgagcggc
    23341 gttttgttcg acggcggcgg cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg
    23401 aacgcaataa acggcggcaa cctgcggcct gccttcggtc agtgtgccgg ttttgtccag
    23461 cacgacggca tcgacgtggg cggcttcttc cattgccgcc gcgtctttaa accaaatacc
    23521 gtgtttcacc gctttgccca tgccgaccat aatcgcggcg ggtgtagcca gaccgagcgc
    23581 acacgggcag gcaatcacca aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc
    23641 cttaatcagc caagtaacaa taaaagtcaa caacgcgatg cccacgacgg caggcacgaa
    23701 caccgccgcc gccttgtccg ccacgcgcgc aatcggtgct ttactgcctt gtgcttcaga
    23761 gagcgcgttc atcatgtcgc cgagctgggt ttggctgccg agctgcgtgg cgcggtacac
    23821 cacactgcct tcggtcatta acgcgcccgc caacactttg ccgcccgcct ttttttcttc
    23881 aggattggat tcgccggtaa gatggctctc gtccgcccaa ccgctgccgc tttcaatgat
    23941 gccgtctgcg gcaatgcgtt cgccgtggtt ggcgcggata aggtcgccga tttgcacttg
    24001 gtcgatggga agctgtttcc attcgccgtt gcgttgcacg ttgacttggg ttggtgtaag
    24061 tttgagcagc aagcccaagc tgttgaggct ggattttttg gtacggtgtt ccaaaaattt
    24121 gcccagtgac acaaaaccaa tcaccatcac gcccacttca aaatacacat gcgccatacc
    24181 gtacgccgcg tgcgggctga aaaacagcat atagacggaa tacaggtaaa tcgagaccgt
    24241 gccgatggta accagcacgt ccatattcgc cagtccgccc ttaatgctcg cccacgcgct
    24301 tttgtaaaat ggcaccgcca gccaaagttg caccacactc gccaatgcga actgccacaa
    24361 cggcggaatc atccaatcgt gtctgccgat catcatcccc gccatgccga taaggaacgg
    24421 gacgttgatg gtgaacagca gccacagccg ccagccgata tggtgttctg cttcgggttg
    24481 cggcaatgta tcttccgttt tttccttcgc gccgtaaccg gttttctcaa tgattttggc
    24541 aatgtcggct actgaggttt tgctgtcgtc aaacactacc tgcgcctctt cgctggcgaa
    24601 gtttaccccc gccgattcga caaaatcttt tttgttcaac actttttcaa tgcgcgaagc
    24661 gcaggcctgg caggtcatgc cttcgatttg gaaacggatt ttttgttgca taagcgtgat
    24721 tcctgctttg atattcagac ggcagttaag gctgcgggat gccgtctgag cgccggtatg
    24781 gtcgaaattt ctcaaacgcg ccgataattc gagtagaatc cgaaaacttg acacagcaaa
    24841 ggaaaaacaa tgtcccaaca ccgcaaactg attattttgg gttccggccc cgccggatac
    24901 accgccgccg tctatgccgc gcgtgccaat ttaaaccccg tcattattac aggtatcgcg
    24961 caaggcgggc aactgatgac caccaccgaa gtggacaact ggcctgccga tgccgacggc
    25021 gtgcaagggc cggaattgat ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa
    25081 atcatttttg accaaatcaa cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc
    25141 gatatgggcg agtacacttg cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac
    25201 ctcggtttgc cgagtgagga agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc
    25261 gacggtttct tctacaaaaa ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt
    25321 gaagaggcac tctaccttgc caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc
    25381 gagttccgtg ccgaaaaaat catgatcgac aaactgatga aacgcgtgga agagggcaaa
    25441 atcatcctca agctggaaag caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac
    25501 ggcgcattat taaaaaacaa cgacggttct gagcaacaaa ttgccgtcag cggcattttt
    25561 atcgccatcg ggcacaagcc gaataccgat attttcaaag gtcagttgga aatggacgaa
    25621 gccggttacc tgaaaaccaa aggcggcacg gcggacaatg tcggcgcaac caatatcgaa
    25681 ggcgtatggg cggcgggcga cgtaaaagac catacctacc gtcaggcgat taccagcgcg
    25741 gcttccggct gtcaggcggc tttggacgcg gaacgctggc tgggcagcca aaacatttga
    25801 atgccgtctg aaaccgaaat tttcggcgga atgcaaattt ttcgcaccaa atccttgatt
    25861 ccgtcggcaa accccaatat aatgcctgct ctttgaacct tgccttctgc tttcgcatgg
    25921 cagaaggctg tttttaccat ccataaggag ataacatgcg tcattacgag atcgtgttta
    25981 tcgttcatcc tgatcaaagc gagcaagtgc ctgctatggt tgaacgttac aaaaccatga
    26041 ttgccgaagc aaacggtaag attcaccgtt tggaagattg gggtcgccgc caactggctt
    26101 acccgattaa caaaatccat aaagcccatt acgttttgat gaacatcgaa accactcccg
    26161 aagtggttga agagctggaa accgcattcc gcttcaatga tgccgtattg cgccatctaa
    26221 ccatcaaaac caaacacgcc gttaccgaat catcccctat gttgggtggt gaaaaggcta
    26281 agaacctgtt gagcggcgcg tctgaagaag cggtcgccca ataattggga ttcaataatc
    26341 ttgtttcgct tgccgtgtta attgaaaagg ttttccctat tcgatatacg cctgccggaa
    26401 tccctgtttt agatattatt ttaaagcacg aatcgtggca ggaggaaaac gggcagcaat
    26461 gccttgtcca attggaaatt ccggcacgga ttttaggcag gcaggcggaa gagtggcagt
    26521 atcggcaagg cgactgcgca acagtcgaag gttttttggc tcaaaaaagc agacgttccc
    26581 tgatgccgat gctcaggata caaaatattc aagaatataa aggttaaacg acaatggctc
    26641 gtcaatcatt caaacgtaga aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg
    26701 attacaaaca agttgatttg ctaaaagact ttatctctga aaacggtaaa atcattcctg
    26761 cacgcatcac aggaacgaaa gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg
    26821 cacgcttcct ggctctgctg ccttacaccg accaacacaa ataattttgg agattgaatc
    26881 atgcaaatta ttctgttaga aaaaatcggc ggtttgggca acttgggcga catcgtaacc
    26941 gttaaaaacg gctatgcccg caactttttg attcctacgg gtaaggcaaa acgtgcgacc
    27001 gaagcgaata tgaaagagtt tgaagcacgc cgtgcagaat tggaagccaa acaggctgaa
    27061 attttggcag atgcccgagt ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct
    27121 caaaaagccg gtgtggacgg tcgcctgttc ggttccgtta ccaatgccga cattgctgct
    27181 gcaatcgttg ctgccggcat cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct
    27241 ctgaaagccg ttggcgagta cgaagtggaa gtggctttgc acactgatgc cgttgctaaa
    27301 attaccgttg ccgtcgttgc cgcaaccgag taattgaaat tatcagatgc cgtctgaaac
    27361 cctgccgatt atcggaatgt ggtttcagac ggtatttgtt ttgttttccg tatattctgc
    27421 aaaaatcggg atggttgttt ttatagtgga ttaaatttaa accagtacag cgtacctggc
    27481 ttcaatatac tcaataggat aggtctcttc ctaaccgaaa aaataattgt cttttccctt
    27541 gcaaataatt gaaatcaaca gattactaaa acacagccaa aatattcaat tgacaaaaac
    27601 ttttctgatt ttgagaaatc tgtgaaaatc aggggattat ttattttgaa aatggtatcg
    27661 ggcataaaat ttggctgtgt ttcaatatgg tgctgataac atactttgtt tcaaaatgtt
    27721 caataggata acttcctgat tgaccgaaaa ataatggcct tttcccttgc aagcagttga
    27781 aatcaacaga ttgtcaaaat acatcctaaa aatttaataa cttatatata tgattgcaaa
    27841 ggttgcggct tgtatgccgg tcattatggc ggaaataccg tttgagacct ttgcaaaatc
    27901 ccccaaaatc ccctaaattc ccaccaagac atttagggga tttctcatga gcaccttctt
    27961 ccggcaaacc gcacaagcca tgattgccaa acaca
//
LOCUS       DAXIEB010000012        26950 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000012 DAXIEB010000000
VERSION     DAXIEB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.012"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            170..499
                     /locus_tag="ACFOA3_000456"
     CDS             170..499
                     /locus_tag="ACFOA3_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6897515.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLRWRLIKTK
                     FSAHFPHAENLSASKQRRHERGIWQRCFYEHTVRDETDLQHRADYIHSNPIKQRMSRA
                     KTESRKK"
     gene            complement(736..1626)
                     /gene="sucD"
                     /locus_tag="ACFOA3_000457"
     CDS             complement(736..1626)
                     /gene="sucD"
                     /locus_tag="ACFOA3_000457"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6897516.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(1637..2848)
                     /gene="sucC"
                     /locus_tag="ACFOA3_000458"
     CDS             complement(1637..2848)
                     /gene="sucC"
                     /locus_tag="ACFOA3_000458"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6897517.1"
                     /translation="MEADSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            complement(2902..3189)
                     /locus_tag="ACFOA3_000459"
     CDS             complement(2902..3189)
                     /locus_tag="ACFOA3_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897518.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(3314..4747)
                     /gene="lpdA"
                     /locus_tag="ACFOA3_000460"
     CDS             complement(3314..4747)
                     /gene="lpdA"
                     /locus_tag="ACFOA3_000460"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6897519.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            4944..5060
                     /locus_tag="ACFOA3_000461"
                     /pseudo
     CDS             4944..5060
                     /locus_tag="ACFOA3_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5112..6305)
                     /gene="odhB"
                     /locus_tag="ACFOA3_000462"
     CDS             complement(5112..6305)
                     /gene="odhB"
                     /locus_tag="ACFOA3_000462"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6897520.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     APDAAQNNAAMPAATKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVA
                     LPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEK
                     EHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPIL
                     RDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQ
                     SAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPAR
                     LLLDL"
     gene            complement(6405..9233)
                     /locus_tag="ACFOA3_000463"
     CDS             complement(6405..9233)
                     /locus_tag="ACFOA3_000463"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6897521.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADAYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(9438..10721)
                     /gene="gltA"
                     /locus_tag="ACFOA3_000464"
     CDS             complement(9438..10721)
                     /gene="gltA"
                     /locus_tag="ACFOA3_000464"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6897522.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(10833..11081)
                     /locus_tag="ACFOA3_000465"
     CDS             complement(10833..11081)
                     /locus_tag="ACFOA3_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6897523.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(11085..11792)
                     /locus_tag="ACFOA3_000466"
     CDS             complement(11085..11792)
                     /locus_tag="ACFOA3_000466"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6897524.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(11912..13675)
                     /gene="sdhA"
                     /locus_tag="ACFOA3_000467"
     CDS             complement(11912..13675)
                     /gene="sdhA"
                     /locus_tag="ACFOA3_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6897525.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDANTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(13678..14019)
                     /gene="sdhD"
                     /locus_tag="ACFOA3_000468"
     CDS             complement(13678..14019)
                     /gene="sdhD"
                     /locus_tag="ACFOA3_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6897526.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(14013..14390)
                     /gene="sdhC"
                     /locus_tag="ACFOA3_000469"
     CDS             complement(14013..14390)
                     /gene="sdhC"
                     /locus_tag="ACFOA3_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6897527.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(14669..16072)
                     /locus_tag="ACFOA3_000470"
     CDS             complement(14669..16072)
                     /locus_tag="ACFOA3_000470"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6897528.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMDSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(16321..17058)
                     /locus_tag="ACFOA3_000471"
     CDS             complement(16321..17058)
                     /locus_tag="ACFOA3_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6897529.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(17335..19611)
                     /gene="metE"
                     /locus_tag="ACFOA3_000472"
     CDS             complement(17335..19611)
                     /gene="metE"
                     /locus_tag="ACFOA3_000472"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6897530.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGKF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(19747..20625)
                     /gene="metF"
                     /locus_tag="ACFOA3_000473"
     CDS             complement(19747..20625)
                     /gene="metF"
                     /locus_tag="ACFOA3_000473"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6897531.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            20825..21100
                     /locus_tag="ACFOA3_000474"
     CDS             20825..21100
                     /locus_tag="ACFOA3_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6897532.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            21100..21225
                     /gene="ykgO"
                     /locus_tag="ACFOA3_000475"
     CDS             21100..21225
                     /gene="ykgO"
                     /locus_tag="ACFOA3_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6897533.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(21486..21686)
                     /locus_tag="ACFOA3_000476"
     CDS             complement(21486..21686)
                     /locus_tag="ACFOA3_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897534.1"
                     /translation="MIGRLFRIFFFFALAALIIKRLFSRRQKRALCEVAKISAWVLLG
                     SAAATLFWYLVMLYFKHIPDSY"
     gene            21846..22985
                     /locus_tag="ACFOA3_000477"
     CDS             21846..22985
                     /locus_tag="ACFOA3_000477"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6897535.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAVIGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            22982..23563
                     /gene="metW"
                     /locus_tag="ACFOA3_000478"
     CDS             22982..23563
                     /gene="metW"
                     /locus_tag="ACFOA3_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6897536.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(23585..23691)
                     /locus_tag="ACFOA3_000479"
                     /pseudo
     CDS             complement(23585..23691)
                     /locus_tag="ACFOA3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            23785..24621
                     /locus_tag="ACFOA3_000480"
     CDS             23785..24621
                     /locus_tag="ACFOA3_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6897537.1"
                     /translation="MKNTTSSLLLWLAAIMLAACSPSKNDKTKEVGASAASSSASSAS
                     SQTDLQPAASAPDNVKQAESAPPSNCTGLHPASGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(24671..25231)
                     /gene="efp"
                     /locus_tag="ACFOA3_000481"
     CDS             complement(24671..25231)
                     /gene="efp"
                     /locus_tag="ACFOA3_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6897538.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(25274..26422)
                     /gene="earP"
                     /locus_tag="ACFOA3_000482"
     CDS             complement(25274..26422)
                     /gene="earP"
                     /locus_tag="ACFOA3_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6897539.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWYAEAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFEQPSAPEKLAAFVSKHQKIR"
     gene            complement(26441..26719)
                     /locus_tag="ACFOA3_000483"
     CDS             complement(26441..26719)
                     /locus_tag="ACFOA3_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897540.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            complement(26730..>26950)
                     /locus_tag="ACFOA3_000484"
     CDS             complement(26730..>26950)
                     /locus_tag="ACFOA3_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897541.1"
                     /translation="QTDAPGFQYARACCRLVLRHRRQRPIFRGTPVSGVFKGSAVRCF
                     PAARLQTASPVRPLDKACAWGDNGVLLF"
BASE COUNT         6599 a         6830 c         6742 g         6779 t
ORIGIN      
        1 ccttaaacaa caagccattc cttctccaac gctcaaacca aaaacaaaaa tcctatggcg
       61 cgttatcgca gaaacttcat tgccggcggc acattctttt tcaccgtcaa actcgccgac
      121 ccgaaatcgc gcctgcttgt cgaacatatc gacttcgcgg gcggcttata tggatgtgca
      181 aaaacaatat tcctttgaaa ccgtcgccgt atgcgtgctg ccgaaccata tccacgccat
      241 ttggacgctg ccacccgacg atgcggatta ttccctgcgc tggcggctga ttaaaaccaa
      301 attctccgca catttccctc atgccgaaaa cctgtctgcc agcaaacagc ggcggcacga
      361 acgcggtatt tggcaacggt gtttttacga acacaccgtg cgcgatgaaa cggatttgca
      421 gcatcgtgcg gactacatcc attccaatcc tatcaaacaa agaatgtcgc gtgctaaaac
      481 agaatcccgc aaaaaataat attagtcttt tcaaatgcga tacttatcca ttcccggact
      541 tcaaacaagg cgtgtgcaac ctgacttacg ggtttgctcc tgtttgaccg ataaatattc
      601 tgactgcctc aaagggcaaa aaataatgtg ggtatgttga ggctgcctct ttttgaaatg
      661 acaaacaaaa tgccgtctga aacctgattt ccggtttcag acggcatttg atgttcaatc
      721 agttgtcaaa cctgattatg ccaaaccttt tgttttcaac acttccagca tggtagtgcc
      781 caactcggca gggctgcggg tgtaagcgat accggctttt tcgaaagcgg cgaatttttc
      841 ctcggcagta cctttaccgc cggaaataat cgcaccggcg tggcccatac gtttgccttt
      901 aggagcggta acaccagcaa tgtaacctac aacaggtttg gttacgttgg attggatgta
      961 ttcggctgct tcttcttccg cagtaccgcc gatttcaccg atcatgatga tggcgtcggt
     1021 atctgggtct tcttggaaaa gtttcagtgc gtcgatttgg ttcataccgg gaatcgggtc
     1081 gccgccgata ccgatacagg ttgattgacc caagcccagt ttggtggttt gtgccacggc
     1141 ttcgtaagtc aatgtaccgg aacgggaaat aataccgatg cggcccggag tgtggatgtg
     1201 gcccggcata ataccgattt tgcactcgcc tggggtaatc acgcccgggc agttagggcc
     1261 gaccaagcgg gtaccgttgc cgttggtttc caagtaacgt ttggctttga gcatgtccag
     1321 agtcggcacg ccttcggtaa tcacaaccac caagcctacg ccggaatcaa cggcttcaac
     1381 gatagaatcc aacacaaacg gagcgggaac gtaaattaca gatgcatccg cgccggtttc
     1441 tttaacggct tctttcatgg tgttgaacac gggcaggttc aggtgggttt gaccgccttt
     1501 gcccggggtc acgccgccga caactttagt gccgtaagcc agagcttgtt cggagtggaa
     1561 agtaccgttt ttaccggtga aaccttgaac caatactttg gtgtctttat taatcaatac
     1621 gctcattctt ttctccttag gcgtttacgg ctgcgacaat tttttcggct gcgtcattca
     1681 ggccgtctgc agaagtcagt ttcagacctg attcgttcag gattttcgcg ccgagttcgg
     1741 cgttgttgcc ttccaaacga acaacgacag gaacgtcgac gttgatttct ttaacggctg
     1801 ccacgatggc ttccgcaatc atgtcgcaac gtacgatacc gccgaagatg ttgatcaata
     1861 cgccttgaac ggatttgtct tccagaatca gtttgaacgc ttcaaccacg cggtctttgg
     1921 ttgcgccgcc gccaacgtcc aagaagttgg caggttggcc gcctttcagt ttgatgatgt
     1981 ccatagtggc catcgccaaa ccggcaccgt tcaccataca gccgatgttg ccttccaggg
     2041 caacatagtt caggtcaaat tcagaagctt tcaactcgcg ttcgttttct tgagatttgt
     2101 cgcgcaattc ggcgattttc ggcaggcggt agagcgcgtt gctgtcgatg ccgattttgc
     2161 cgtccacgca ggcgagcgcg ccgttttcgc gaactgccag cgggtttact tcaaacaggg
     2221 cgaagtcatt ttcgacaaac gctttgtacg caccggtcat cagtttgacg aactcgttga
     2281 tttgtttgtc tttcaagccc agttggaacg caacttcgcg agcttggcaa ggttgcaggc
     2341 cgaccagcgg atcaacggtt actttgaaga ttttttcagg agtttcggca gcaacttttt
     2401 cgatttccac gccgccttcg gtagaggcca tgaatgtaat gcggcgggta gaacggtcaa
     2461 ccactgcgcc caagtacagc tcggtttgaa ccggatacat gtcttcgcaa accaaaacac
     2521 tgttgacagg ttggccgttg gcatcggttt ggtaagttac caagttggtg ccaatcaggc
     2581 tttcagccac ttctttagct tcttcgcggc ttttaacgac ttttacgccg cccgctttac
     2641 cgcggccgcc ggcgtgtact tgtgctttga caacagcgaa tttgccgccc aatttgtcgt
     2701 aagctgcagc ggcttcttcg ccgttgtgtg ccaaaatacc gccttgtacg ggcaaaccgt
     2761 agctagccag cagttcttta gcctgatact cgtgtaaatt catggatttc tcctttagat
     2821 tagtggaatg ccccgagtct gcctccaaat ggcaagcccg tgcgtttcag actacctttc
     2881 ggcggtcgga aaatcgtcgt ttcacttgct gaaaggcaac cacatccgcg ccagcagata
     2941 catggctgca ccgcctcccc cgagaataaa aaagacgatg ccttttttac gcgaattcgg
     3001 acagagcaga taaacggcaa ggctgaaact gataaacaac aatgccacgc gccaaaccaa
     3061 ttctttatct ttgaaacggt tgagcgtata agtttcggtc atcatcacat acatctgcca
     3121 caatgcggcc atacacatgc tgatgatgcc catcggtata aagaaaatac cgactatttc
     3181 tttgttcata acgaatgtaa atgctgtttg tttgtctgac ggaattataa atcagaaaac
     3241 ggagctctcg gaacattcaa tttgtcgaag gcattaaggt cgtctgaaaa atttcagacg
     3301 accttaatgt ctatcaaccg tgcaaagcgc gtttgtcggc cgccaatgca gcttcgtgaa
     3361 ccacttcgga caaggttggg tgggcatgga taatgcgggc gatgtcttcg ctgctggcga
     3421 agaattcgag cgcagtcacg ccttcggtaa ccaattcgct gacaaccgga ccaatcatgt
     3481 gtacgcccaa gatgcggtcg gttttggcgt ctgccaacac tttaaccgta cctttggctt
     3541 tgcccattgc caatgcgcga ccattcgcac caaaacctga agtacctttt ttgtactcca
     3601 cgccttcggc tttaagctgt tcttcggttt tacccaccca agcgatttca ggatcggtgt
     3661 aaatcacaaa cggtacgttg ttgaaatcga tatgcggttt ttgaccggca atgcgctcgg
     3721 caacggcaac gccttcgtcg ctggctttgt gtgccaacat cgggccgcga accacgtcgc
     3781 cgattgccca tacattaggc aggttggtgc ggcattcgcc atctactttg ataaagccgc
     3841 gctcatcttt ttccaagccg acggcttcag cgttcaggcc tttggtgttt ggaatacggc
     3901 cgatggcaac gatcagtttg tcgaatactt cggttttggc ttcgccggca gcagtttcgt
     3961 aagcaacgga aacgcctttg ccttcagatt tgatgtcgcc gattttcacg cccaattcga
     4021 tgctcaaacc ttgctctttg gtgaagtatt tgaaggcttc tttggcgatt tgttggtcgg
     4081 cggctgccag gaaggtcggc gcagcttcaa gaatggtaac ttccgcaccc acgcggttcc
     4141 atacggaacc catttccaaa ccaatcacgc cagaaccgat cacgccgagt ttggcaggta
     4201 cttcggtcag gttcaatgcg ccttcgttgt ccaatacgtt cacattgtcg atagcgactt
     4261 gtggcagcgg acgcggtacg gaaccggtgg ctacgatgac gtgtttggct tcgataacgg
     4321 ttttctcgcc tttgttatcg acttcgattt ggtaagcatc accattttta ccggcaaagg
     4381 aagccgtacc gaacaggctg gttactttat ttttttggaa caggaatttc acaccgccgg
     4441 tcagtttggt cacgatggca tctttgcgct caatcatttt ggccgcatcg aattttacgt
     4501 cgccgacagt gatgccgtgt tcggcaaact cgtgttgcgc tgcgtggaaa tgttcgctgg
     4561 actgcaacag ggctttggaa gggatacagc ctacgttcaa gcaagtaccg cccaatgcag
     4621 gggcattgcc tgctttgtta acgcctgcat cgacacaggc agttttgaaa cccagttgtg
     4681 cggcacggat ggcggcaacg tatccgcccg ggcctgcacc aatcactact acatcatatt
     4741 gagacatcgt gtcatccttt gcttttagca gtatctcaaa ataaaaacga tacttttcgt
     4801 ttctttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
     4861 catatctgcc gattcattcg aatcaaatgt ttcagacggc ctcaatcgtt cgttgagcct
     4921 attgtagcac gatactacat tacgctacat ctaaatatag tggattaaat ttaaatcagg
     4981 acaaggcgac gaagccgcag acagtacaga tagtacggca aggcgaggca acgccgtact
     5041 ggtttaaatt taatccacta taaaaattca gacggccttt tcattaacaa ataaaaggcc
     5101 gtctgaaacg attacagatc caacaacagg cgggctgggt cttccaacgc gtctttaatg
     5161 gctaccaagg tcaatacagc ttcgcggccg tcaatgatac ggtggtcgta agacagagcc
     5221 agatacatca ttggacggac aacaacttgg ccgttttcaa ccacagcgcg ctctttagtg
     5281 gcgtgcatac ccaaaatcgc agattgaggc gggttgatga tcggggtaga catcatagag
     5341 ccgaaagtac cgccgttggt aatactgaat gtaccgccgg tcagatcttc gatagcgatt
     5401 ttgccgtctt tggctttttt cgcgtaatca acaattgctt gctcgatgtc ggcaatgctc
     5461 atttggtcgg catcacgcag gattggcaca accaaaccgc gtgggctgcc aatcgcaata
     5521 ccgatgtcga agtagccgtg gtacacgatg tctttgccgt caacagaagc attcacaacc
     5581 gggtattttt tcagggcggc aacagcggct ttaacgaaga aggacataaa gcccaatttc
     5641 acgccgtgtt ctttctcgaa tttttctttg tacttcgcac gcaagtccat gattggtttc
     5701 atgttgactt cgttgaatgt agtcagaatg gcgttttctt gttgagaagc caagaggcgt
     5761 tctgcaacac gggcacgcag gcggctcatt ggtacgcgtt cttcaggacg tgcgccggca
     5821 ggaagtgcaa cggcaggggc tgcggctgcg gcaggtttgg cagcggcatt ttgtacgtct
     5881 tctttcaata cgcgaccgtc acggccggaa ccttgcaatg cgttcacgtc aacaccggtc
     5941 tcggcagcca gtttggtggc agcaggcata gcggcgttgt tttgtgcagc atcaggagcg
     6001 gcagcaggag cggcagccgg ggcggcttcg gcaggagcgg ctacgggtgc ttcggcagcg
     6061 gcagtagcag ctgtatcgat gcgtgccaaa acttggtcgg caacaacggt ttcaccgtct
     6121 tgcgctacga tttcaaccaa tacgccggct tgtggagaag gtacttccaa aaccactttg
     6181 tccgtttcga tatcgatcag gatttcgtca cgggcaactg cttcgccaac ttttttcttc
     6241 cattccaaga gcgtgccttc agatacgctt tcagacaaca taggtacttt tacatcaata
     6301 atcattttgt gtctccaatg gccttttcag acggcctgtt gtgtgttttt aattctgcat
     6361 atgggcattc catctgagca gactcagacg gaatgctact tggtttacaa tgccaaagcg
     6421 tcttcaacca attgtttcaa ttgagcaatg tgtttgcttg agtagcccac tgcaggcgat
     6481 gcgctgcttg gacgaccggc ataagacagt ttttgctctt cgctaataac atcttcgatg
     6541 cggtggcgga tttggtagaa cgcgccttgg tttttcggct cttcttgcgc ccaaaccaca
     6601 gattttgcgt tcggatattt cgccagttcg gctttaacct catcgtatgg gaacggatac
     6661 agctgctcaa cgcggacaat cgcaacatca tcttccagtt tacgttcggc tcgaccggct
     6721 tccaagtcat agtaaacctg accggcacac aataccacgc gtttcacgct gtcgttgctt
     6781 gcgcgttcgg cggtatcgcc gataaccgga cggaaagtcg aaccttcggt gaagttttcc
     6841 agcgggctca ttgcaccttt gaatcgcaac aggcgtttgg acatgaaaat cactagcggt
     6901 ttgcggtatg aacctaagac ttgacgttgc aagaggtgga acatttgcga cgcttcagat
     6961 ggcatgatga cttgcatatt gttctcagaa cacagttgca accaacgttc tacgcgtgca
     7021 gaagagtgct cagggccttg accgtcgtaa ccgtgcggca ggatggtggt cagaccgcac
     7081 aagcgacccc atttggtttc gcctgaagac aggaattgat caatcgtcac ttgcgcgccg
     7141 ttggcgaaat cgccgaattg cgcttcccaa atggtgagct tgtcaggtgc ggagcaggca
     7201 aagccgtact cgaacgccat cacggcctct tcgttcaaaa tggagtcgat aaccaggaac
     7261 tcgcccattc cttcgcccat atggcgcaga ggaacataag taccgtcgtc ccatttttcg
     7321 cgtttttgat cgtgcaatac ggcgtggcgg tgcgagaacg taccgcggcc ggagtcctca
     7381 ccggagatac gcacgccatg acctttggtt acgaggctgg cgtatgccaa ggtttcggcc
     7441 ataccccaat ctatggcctg tttgccggat gccatggctt tacgcgcttc aatcacacgt
     7501 tttgcagtcg gatgcagggc aaagccttcc ggtacggcgg taaacttctc agtgagacgc
     7561 tcaatatctg cggcaggcaa accggtttcg atgtgttcgc gccaatcttt gccttggtat
     7621 ttgctccagt cgatttgcgt gcgttgaaag ttgctcaacg ttgtttgctc gacgtgttcg
     7681 cctttgtcca aagcatcacg gtaggcttgg atgtaagcat cagcttctgc ttgagttacc
     7741 acgccttcgg caatcagttg ctcggtgtac aaagcacgcg caccggggtg ttgcgatact
     7801 tttttgtaca tcatcggttg ggtcaaggtc ggatcatcgc cctcgttgtg accccattta
     7861 cggtagcaga caacgtcaat cacgatgtct ttatggaatt ttttgcggta atccaaagcg
     7921 gcttggatgg caaagcaaac gcgctcagga tcatcgccgt tcacgtggat aaccggagcg
     7981 gaaaccattt ttgcgatatc ggtacagtgt acggttgaac gggtatcgcg gatatcggaa
     8041 gtggtaaagc cgatttggtt gttgatgacg atatgaaccg taccgccggt ggtataaccg
     8101 cgtgttttag acaggttgaa tgttgcttgg ttgacaccca gaccgataaa tgcggagtcg
     8161 ccgtgaatca ataccggcaa gactttgtcg cggccgtttt cgcccaaacg tttttgtttg
     8221 gcgcgcgcag aaccttccac caccgggttg acgatttcca agtgtgacgg gttgaacgcc
     8281 aaagaaacat gcatcgggcc gtgcggggta gcaatatcgg agctgaagcc catatggtat
     8341 ttcacgtcgc cgctgggcag tttgatttcg gcacgacctt caaattcggc aaacaaatcg
     8401 ccgggttttt tgcccaaaat gttcaccaaa acattcagac ggccacggtg cgccataccg
     8461 atgatgactt cttccacgcc gtctttaccg gcgttttgaa tcaggtagtt caaaccggca
     8521 atcgcgcttt cgccgccttc gacaccgaaa cgtttctgac cgacatattt ggtatgcaga
     8581 taacgttcca aagtttcggc agcagtcatc tctttcaaga tacggcgttt ttgatcggca
     8641 ttgtaatgcg gtgtggacaa tacgctttca aaataattgc gtacccagcg gcgctcttcg
     8701 gtattgggaa tatagatata ttccaatgcg atgtggccgc agtaggtttg tttgaggttg
     8761 ctgatgattt gggacaaagg cagtttgccg cgattggcaa aatcgccctc gcccatattg
     8821 aattgaagcg ccatatcggc atctgacaga ccgtggaatt tcggatcgag ggcttcaata
     8881 tcgcgcgggg ggatacgttt gagcggatca agttgggctg cacccacgcc ttggatacga
     8941 taggcggaaa tcagccgtaa aacgctgact tgctttttca gcattgcctc atccgcaccg
     9001 cccgcaacgg cagatgcaat tttctttttc gccaaagtaa caaatgattc gcgaatcggt
     9061 gtgtgtgcga catcgacagc aaccgtcccc ggctgtttgc tcaaatcggt gaaatactgc
     9121 ttccattttt catcaaccgc atcggggttt tccaaaaaag cctcgtacaa ttcctcaatg
     9181 tagggtgcgt tcgaaccgaa caggtaagag aaattgagtt tttcgtccat catggcgtgc
     9241 gctcttttct ttaaaatata aacaaagggt agaagtccgc gtcctgacgg ctaaactgat
     9301 gccgtctgaa aagacatttt attctaccct attttaaacc ggattgcttg tcttgatttc
     9361 atccgactac aatcagacgg attcgggaaa caatccggtt ctctgatatt gcctgtttga
     9421 caatatatta atgtatgtta cctctcgcct ggcggcacat aatcgcggcg ttccgaaccg
     9481 gtataaagct ggcgcgggcg gccgattttc agcgaaggat cgctaatcat ctcgtgccag
     9541 tgcgaaatcc agcccacgct gcgcgacagg gcgaagatga cggtaaacat ttcggtcggg
     9601 atgcccagcg cggacaggac gatgccggaa tagaaatcga cgtttggata cagtttgcgt
     9661 tcgataaaga acgggtcttt cagcgcaatc tgttccaatt ccatcgccag tttgaatttc
     9721 ggactgtctt ccaagcccaa ttccttcaaa acttcatagc aggtttcgcg cataatgctg
     9781 gcacgcggat ccatattgcg gtacacgcgg tgaccgaagc ccatcagacg gtatttgcgt
     9841 tgtttcacac cttccatgta tgcggcaaca ttagacacat cgccgatttc gtccaacatt
     9901 ttcaacacgg cttcgttcgc accgccgtgc gaagcacccc acaggcaggc gataccggca
     9961 gcaatacagg caaacggatt cgcacccgaa gagcctgcca gacggacggt tgaagttgag
    10021 gcgttttgct cgtggtcggc atgcaaaata aagatgcggt cgagcgcgcg tgccaaaacg
    10081 ggattgggtt tgtagtcttc acacggcgtg gcgaacatca tatgaaggaa gttttcggaa
    10141 taagaaagat tattcttcgg ataattgaac ggcagaccgt ttgaatagcg gtagcacatt
    10201 gccgcaatgg tcgggatttt agaaatcagg cggtaaatcg cgattttgcg gtgttcggga
    10261 ttgctaatgt ccaagctgtc ttggtagaac gcagacagtg cgccgaccac gccgaccatc
    10321 atcgccatcg gatgcgcgtc gcggcggaag ccccggaaga accaagtcag ctgttcatgc
    10381 accatcgtgt ggcggcggac ggtattgtca aattctgcct tttgctcggg agtcggcagt
    10441 tcgccgtaaa tcaacaggta gcagacttcc aaataatcgg acttttcggc cagctgctcg
    10501 atggggtatc cgcgataata aagcaagcct tgatcgccgt cgatgtaagt aattttagac
    10561 tcacagcttg cggttgaaac aaatccgggg tcgaaggaaa acaaacctgt attttttgtc
    10621 agcccccgaa tgtcaaccac atcgtgcccg atgctggctt ccaataccgg cagctccaaa
    10681 cctgcctgac ccggtacgtt gagtttgatt gatttggaca tattattgct cctttgcaag
    10741 ttataaccca cgttactgca tccgcagcac gttctttgcc ttcagacggc ttttgcattt
    10801 tgaaatctgc ctgcaggcag atttcagatg cctcatgccc gtctgatttt ttcaagcatc
    10861 gggataaggt gccctttgtc cgtttccgaa tgcccgttaa tcaaggcaag caattcttga
    10921 tcttgaaatt caaggatttc ggaaaactcg gacagctctt tatcgctcaa atgctcgaat
    10981 tctttttcca taaacctgcc gaagattaaa tctaactcca acaatccccg gcgggtttga
    11041 aaacggattt tccgtttggc aatatcgtca aaaaccatca tttcttaaac ggcccgtttc
    11101 aacataatct ctttaatctt accgatggct cgggtcggat tcaagtgttt aggacatacg
    11161 tctacgcagt tcataatggt gtggcaacgg aacaaacggt atgggtcgtt cagattatcc
    11221 aaacgctcat tagtgatggt atcacggctg tccgcaatga aacggtaagc attcagcaaa
    11281 ccggacggac cgacgaattt atcagggttc caccaaaatg acgggcaggc agtcgaacag
    11341 caggcgcaca aaatacactc gtacaaaccg tccaactctt tacgctcttc ctgagtttgc
    11401 agacgctctt tgtccgcatc aatcggatta tcgttgacaa cataaggttt gacggaatgg
    11461 tattgtttga agaactgggt catatccaca atcaggtcgc ggataacagg cagacctggc
    11521 agaggacgaa ttttaaccgg ctgtttcaag ccacgcagat cggtcaaaca cgccaagccg
    11581 tttttgccgt tgatgttcat accgtcagaa ccgcaaatcc cttcgcggca ggagcggcgg
    11641 aaagacaagg tatcgtcttg tgctttcagg cgcactaaag cgtccaaaag tttcacgtcg
    11701 gtcggttcca attccaactc gtaacgctgc atataaggct tggcatcaac atccgggttg
    11761 taacggtaaa tttcaaaact cattttttcc atgagaagtg ttccttttct catcaactgc
    11821 ccaatgattt tgtattataa attcaagcag ttcaatgtgg gtatggttag atttcagacg
    11881 acctttgagg cgaagaccgt ctgaaaacgc atcaataaac gcgcttggcc ggtttgatgt
    11941 attccacgct caaaggcttg gtgtgcaccg gtttgtagga caaggtattg gcatctgaat
    12001 ggtacagcgt atgtttcatc cagttttcat catcgcgctc aggatggtcg tctgaagcgt
    12061 gcgcaccgcg tgattcttta cgtgcttcgg cagacaccaa agtcgctttc gccacttcaa
    12121 ttaggttatc caattccaaa gcctcgatac gcgcggtatt ccacactttg ctcttgtctt
    12181 tgatttcggt acgtttcaca cgctcggcaa tcgccatgac ttctcgaacg cctttgctca
    12241 gaatctcatc agtacggaac acgccggcgt gcaattgtac ggagcgttgc agttcgcggc
    12301 gcaatgcatc aacgttttca ccatcggttt ggctgtccaa acgctcgata cgttggcgag
    12361 tcagctcgcc cgcatccgca ggcaaaggtt tccagtcgct ttgctctttg atgaatttaa
    12421 tcatgctgtc gccggcagct ttaccgaata ccaccaagtc caacagggag ttggtaccca
    12481 agcgattcgc accgtgtacg gaagcacagg cgcactcacc tgcggcatac aggcctttta
    12541 caggcacttc gtattcgtct ccttgcggca caacgacttc gccgtggtaa ttggtcggaa
    12601 tgccgcccat catatagtgg gtagtcggca caacgggaat cgggtcttta atcggatcga
    12661 taccggcgaa ctgaatggaa atctcgcgga tgcccggcag tttttccata attttttctg
    12721 cgccgatatg gtcgattttc agtaagacat ggtctttgtt tttaccgcag ccgcgacctt
    12781 cgtagatttc catcgccatc gcgcgggaaa caacgtcgcg agaagccaag tcttttacgg
    12841 tcggcgcata gcgttccata aagcgttcgc cgtcggcatt caacagaata ccaccctctc
    12901 cgcgtacgcc ttcggtaatc aacacgcccg cacctgccac gccggtcggg tggaattgcc
    12961 aaaattccat atcttccaac ggaatgcccg cacgggcgca aatgcccaaa ccgtcgccgg
    13021 tattcatata ggcattggtg gaagacgcat aaatacggcc gccgccgccg gtagcaaaca
    13081 ttacggcttt ggcgtggaaa atataaactt cgcccgtttc catttccatg gcggttacgc
    13141 cgacgacatc gccgttttca tcacgaatca aatcttgcgc cgtccattcc acaaagaatt
    13201 gcgtattggc acggacgttt tgttggtaca acgtatgcaa catcgcatga ccggtacggt
    13261 cggcaaccgc gcaagcacgt tctaccgcgc gtttaccgtg ttcggcagta tgaccgccga
    13321 aaggacgctg ataaatttta ccgctttcaa cgcggtcaaa aggcataccc atgtgttcca
    13381 actcaatcac cgcttcaggc gcggcgcggc acataaactc aatcgcatct tggtcgccca
    13441 accagtcgga acctttcacg gtatcgtaca tgtgccagtc ccaacggtct tcctgcacat
    13501 tacccaatga agcagaaata ccgccttgag ccgctacagt atgagaacgg gttgggaaca
    13561 ctttagacaa aacagcacaa ttcagaccgg atttggataa ttggagggct gcgcgtaaac
    13621 ctgcaccacc accgccgaca atcacggcat caaacttgcg aacaggaaaa cccatactta
    13681 cccccaaatt actttaattg aatacaccaa gcagccgacc agccagacga tggtggcaac
    13741 ctgcaaaaac aaacgcacgc cgaagggttt gatatagtcc atccacaaat cgcggatacc
    13801 cacccaagcg tgcaagaata cggcgatgaa gctcacttgg gtaaatactt ttacccaagt
    13861 ttgactaaaa aatgcctgcc atgccgaata ttctttaggc agggaaaata gaaccactaa
    13921 aagtgcaacg gtataaatca acataataac cgcagtcgca cgttgcatcg cccaatcgcg
    13981 caaaccgtaa tgggcaccgg tcaatttacg ttctaccata acaacgctcc caaaacgaca
    14041 gtcaaaacca atgcagaagc aaatacggct ttagcggtat tgcgcgcagt attcagctca
    14101 aggcctttgt gcgcatccaa aaataaaaag cggataccgg cgagagaatg gtgcagataa
    14161 gcccacaata caccgattaa aaccagcttg accaaaggat gggaaacaat ggcacggtaa
    14221 gtttcaaatg cagactcttg actcagggta ccggacagga aatacagcag gaaaggcagc
    14281 ataataaaca gcccgacccc gctgatgcgg tgaaggatgg aaactatccc gggtatcggc
    14341 agacggatgt tcggcaaatc cagataaaca ggacgcggtt tggcagacat agtcaattcc
    14401 tctgtagtta tatatcgact caaaaaccgt aaacccattg ctacactggg ttacataaac
    14461 gttaatttac ctgttttatc ccattttgaa cagtgtattt taaaaacaaa ctgatagatt
    14521 ttgacaaaca cgcgtggaaa cagcagatta aaagaaagat ttttagtttt gccgccccct
    14581 tcccgacacg gtatcagcat ccgaccccat ccccaaaaac aaaatgccgt ctgaaaaaat
    14641 ttcagacggc attgttttgc catatcggtc aggctttcaa tttcgcatcg gcatcgcgca
    14701 acgcggtacg cagaccttcc tcgataacgg gatggtagaa cggcatatcc agcatttgcg
    14761 gaacggtcat cttcatttga tgtgcccaag ccaacaggtg cgccaaatgt tcggcggcag
    14821 ggcctacgat ttccgcgccg ataaagcggc cggtggcttt ttcggcatac aggcgcatat
    14881 ggcctttgtt caccagcatc acgcggctgc ggccttggtt tttgaacgat acttcgccga
    14941 tgacaaattc gtcggcttgg tattgcgcgg caacctgcgc gtatttcaga ccgacaaagc
    15001 cgatttgcgg actggtaaac accacgccga tggtgctgcg ccgcaaaccg ccgccgatat
    15061 tcgggtagcg gcccgcgtta tcgccggcaa tcttgccttg gtcggcagct tcatgcagca
    15121 gaggcagttg gttggacgca tcgcctgcga tgaagatatg cggaatactg gtctgcatgg
    15181 tcagcgggtc ggcaacaggt acgccgcgcg catctttttc gatattgata ttttccaaac
    15241 cgatattgtc aacgttcgga cggcggccca cggctgccag catatattcg gcaacaaata
    15301 cgcctttttc gccatcctgc tcccaatgga cttctacatt gccgtctgca tcgagtttga
    15361 cctcggtttt agcatccaga tgcagtttca attcttcgcc gaacacggct ttcgcctcgt
    15421 ctgaaacgac ggggtcggaa atgccgccga tgattccgcc caaaccgaaa atttcaactt
    15481 tcacgcccaa acggtgcaat gcctgaccca gttccaaacc gataacaccc ggcccgaaca
    15541 cggcaacgcg cttaggcagc gtatcccatg agaaaacgtc atcgttgata atcaaacgat
    15601 tgcccaaaga ctgccattgc ggcaggatga cgggacgcga accggtagca atcacgaaac
    15661 tttttgccgt aatttgagta tgctcgtcga tttggacggt atgctcgtcg ataaatttag
    15721 ccgaatccat aatgcgcttg tcggcaggcc actcttccac atcggcaacg acaaagccga
    15781 caaaacggtc acgctcggat ttaacgcgct gcatgacctc ttcgccgttg acgacgatgc
    15841 tgtctttgtc caaatgcacg ccgaacgggt cggtatgcaa tgcgtgatga cgcgcctctg
    15901 cggcggcaat caagagtttg gaaggcatac agcccacgcg cgcgcaggtc gtgccgaaca
    15961 cattgttttc aatcaggtaa acattatccg aatgtaaacg ggcattgcga aacgcgccca
    16021 taccggcagt accgccgccg attacgacga catctgcttg aatttttttc ataatctttg
    16081 tccttccgtt ttcgtctgaa acactgccga cacggaatat atcggcttca gacggcattt
    16141 aaatattttc ccaatcaaaa acattattca gataaataaa aacaaaattt cgattgagtg
    16201 caacaatatc caaatcctct tataaatata gagttattcg gagcggtagt gtagaaccgc
    16261 ccgaataact ctatccggta tattagacag tttaatcaaa ctgccttaag caaacagcta
    16321 tcagttttta gccaagtaag cttccaaatc ttcgctgccg ccgatgtatt taccgccgat
    16381 gaagacttga ggagcagtca tcttgccggt aatggcgcga acggaagtaa cggttgcatc
    16441 tttgcccaat acgatttctt cgtaagacaa acctttgtct tgcaaagctt gtttggcttt
    16501 agcgcagaat tggcaacctg gtttagtgaa aattgccaca gactcttgag ccttccaatc
    16561 gggagcaaca aatttcagca tagtatctgc atcggatact ttgaaaggat cgcccggttc
    16621 ttcaggttcg atgaacattt tttcaaccac gccgtcgtta accagcatgg agtaacgcca
    16681 agagcgttta ccgaagccca agtcttcttt accgaccaac atgcccatgc cttcggtgaa
    16741 ttcgccgttg ccgtcaggaa tcatgtagat gttgtcggat tcttcttcgg ccgcccaagc
    16801 gttcattacg aaagtatcgt ttacagatac gcagtagatt gcatcaacgc cgttttcttt
    16861 gaacgcgccg aacaattcat tgtaacgtgg caaatgggaa gaagagcaag tcggagtaaa
    16921 tgcgccgggc agagagaata cgactacttt tttgcctttg aacaaatcat cggtagacac
    16981 atctttccaa gtgtcgccga cgcgggtgcg gaatactacg gaaggtactt tttgaccggt
    17041 acgatcttgc aaagccattt tgagctcctt aatagatagg tttaagaaat gattgtgaat
    17101 cgctgggcat tctaccccga ataagtcgat ttgtataatt tatagttcaa ataatattaa
    17161 ttattcaaaa ctattagtta ccaacctcga ccaacttctt gcagtatata aagtcatgcc
    17221 taaaaaaaac aagtcttatt tgagaaaaat tcttagaaac tcccctacgc tcatttgaaa
    17281 acagaaatgc cgtctgaaaa gtattcagac ggcatttgat cacgttatcc ctgcttattt
    17341 agccaattcg gcacgcagtt tgtgggttac gttcatcatc acttggagtt gttccagagt
    17401 ttctttccag ccgcgtgttt tcaggccgca gtcaggattc acccacagac gctcaaccgg
    17461 cacaacctcg atggctttgc gcaacaggtg ctcaacttcg gcttcagtcg gtacgcgcgg
    17521 gctgtggatg tcgtaaactc ccgggccgat gtcgttcggg tatttgaact tgccgaacgc
    17581 ggtcaagagt tccatatcgg aacgtgaagt ttcgatggta atcacgtcgg catccatagc
    17641 tgcgatggcc ggcaggatgt cgttgaactc ggagtagcac atatgggtgt ggatctgagt
    17701 gctgtcttcg caaccggcag aggacaggcg gaaagattcg ccagcccagt tcaggtaggc
    17761 atcccaatcg gcacgtttca aaggcaggcc ttcgcggatg gcaggttcgt cgatttggat
    17821 gactttgatg ccggcttttt ccagatccaa tacttcgtcg ttcagggcca gtgcgatttg
    17881 tttgcataca gtagagcgag gaatatcgtt gcggacgaaa gaccattgca gaatggttac
    17941 agggccggtc aacatacctt tcatcgggcg tttggtcagg ctttgtgcgt aagtggacca
    18001 agcgacggtc atagcttcag gacggcttac gtcaccgaag ataatcggtg gtttcacgca
    18061 gcgtgagccg tagctttgta cccagccgta ttgggtaaat gcaaaaccgc tcaacaattc
    18121 gccgaagtat tcaaccatgt cgttacgctc ggcttcgccg tgtaccagta cgtccaagtc
    18181 cagtttttct tgctcttcaa ccaccaaggc gatttctttt ttcatcgcgg cttcgtaatc
    18241 ggcggcagac agttcgcctt ttttgaaggc tgcacgtgcc tggcggattt cggtggtttg
    18301 cgggaaagaa ccgatgttgg tagtcggtag cagaggtagg ttcaaccatg cttgttgcgc
    18361 tttgatacgg tcggcaaatg gagatttgcg ttggtctgcg ttggcaggca aatcggccag
    18421 gcgtttggca acgtctgcac gatggatttc gctgctgttg gcacgggagt cggcagcagc
    18481 ttggctggcg gcgagttctt cggcaacaga atcacggcct tcgttcaatg cagctttcag
    18541 aacgcgcaat tcttgggttt tttgcagggt gaatgccaac caagagtaca ggtcgggttt
    18601 gttggctttc agtttttctt caactgacaa gtcaaatgga gtgtgcagca gcgagcaaga
    18661 gctggaaatc cacaaacggt cacccagttt ggcttgcaga ggctcgacag tttccaaaac
    18721 tttgttcagg ttggcgcgcc aaatgttgcg gccatcaatc acgccggcag acagaacttt
    18781 gtcgtagtcg gcgaacgcgt ccagttgctc gggagcgcgt accaagtcga tgtgcagacc
    18841 gtcaacaggc agggatttca acaatgcggc gtgttcggca acagaaccga agtaagtgct
    18901 cagcaggatt ttggcactta ctttgctcaa agtggcgtaa acgtctttgt atgcttctac
    18961 ccattcttta ggcaggtcaa cagtcaaagc aggctcgtcg atttgaatcc actcggcacc
    19021 ggcttcaacc aaagcagtca ggatttcaac gtaaacaggc aacagtttag gcaacaggct
    19081 cagacggtcg aattcgacgg cgcctttttc tttacccacc cacaggaaag tcaacggacc
    19141 tacaacggtc ggtttggctt tcagaccgag ggcttgggct tcttgcagtt gttgaacata
    19201 gtgtttggca ttggctttga attcggtatc ggcgtggaat tcaggcacca agtagtggta
    19261 gttggtgtcg aaccatttgg tcatttcgat agcgaattgg tctttgttac cgcgcgccag
    19321 ttggaagaat tgttccaaag acaggttttg gctgtcgaag ccgaagcggg cgggaatcgc
    19381 gccggtggcg acttgcaggt cgaggatgtg gtcgtagaaa gtgaaatcgc ctacggcaac
    19441 gaaatcggcg ttggcagcgg cctggtgttt ccagtttttc tcgcgcaagt ctttagcaac
    19501 agccagcaat tcttgctcgc tgatttcttt gcgccagtat ttttcttgtg cgaatttcaa
    19561 ttcgcggaag gcgccgacac gcgggaagcc tgagaaatgt aatgttgtca tgttgaactc
    19621 tccagttgga ttttgtaagg accggttcaa tccgaatcgg ccgatattgt aaatcagaat
    19681 ggctcggata gggcggcgtt ttgcttttca aacggcctcg gatgccgtct gaagccgttt
    19741 tcagctttaa gggcgcacgc ctaaaatatg gcaaatggcg taagtcagct cgctgcggtt
    19801 aagcgtatag aagtggaaat ctttcacgcc ttcgcgggac aggactttga ccatatcgat
    19861 ggcgatgctg gcggcaacaa ggttgcgcgt gccttggtca tcgtccaaac cttcatacat
    19921 ttgcgacagc cagcttggga ttttgacatt ggttacttga gccattttac cgagctgctt
    19981 gaagttggta acgggcagga tgccggggac gatttccaca tcgatgccca acatcacgca
    20041 gcggtcgcgg aagcgcaggt agcgttccac atcgaagaag aattgggtga tgacgtggtt
    20101 cgcgcccgca tcgattttgc gtttcaaatt aatcaggtcg gcttgtgcgg atttcgcttc
    20161 gggatgcact tcgggatatg ctgctacaga gatgtcgaag tcggcgacgg agcgtaatag
    20221 cttaaccaag tcttcggcgt aaaacggttt tttctcataa ccggccggct cgtctccgcg
    20281 cagggcgaca atgcggcgga tgccgctgtc ccaataatct ttggcaattt ggcgcaattc
    20341 gtcgggagaa gcgtcgatac cggtcaggtg aggcgcggct tccaagccgg tttcctgttt
    20401 gatgcgtttg acgatgccgt gcgtgcggtc gcgttcgccg gaatttgcgc cgtaagttac
    20461 ggatacgaac ttgggatgca gggtttgcag acggtggatg gagtcccaca gcatcgtttc
    20521 catttgttcg tttttcggcg ggaaaaattc aaacgaaacg ttaatgtcgc ctttcaaatc
    20581 agaaagactg ttatttaacg cattgatttc ttttgcgtaa ttcatggctg cgtacctttt
    20641 attatctttt atatgatgtt tcaggcaaca taataaagtt gcgccaaaca tgagtcaatt
    20701 tcaaaatttt cagatttatc atgaaaattt ctaatatatt acaaaaatag ttaaatttcc
    20761 ttgtacaagc cttctaattt atgataaagt ttgttatgtt ataacaattc aactaaggcg
    20821 atatatgaaa ccgaatattc atcctgacaa ttatcgaacc gtcctttttt tcgacagcag
    20881 cgccaatgaa ggctggttaa tccgctcctg tgccgaaaca cacggaaaaa cgatggtttg
    20941 gacagacggc aaagaatatc cgctgttttc gctggatact tcttccgcat cccatcccgt
    21001 ctatactggc aaacagcgca acgtcaacac cgaaggccgt gccagcaaat tcaaccaacg
    21061 tttccaatcc gtcatgtcat cgtttagaaa ggacaaataa tgcaggttct gtcttcactc
    21121 aaaaccgcca aacaacgcca ccgcgactgt cagattgtca gacgtaaagg caaagtttac
    21181 gtcatctgca aaagcaatcc gcgttttaaa gcgcgccaac gctgaccccc tgcccttcct
    21241 gcttcggttg tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    21301 caaatagtac ggaaccgatc cacttggtgc ttcagcacct tagagaatcg ttctctttga
    21361 gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctataaagca cctcctaccg
    21421 caagcccttt tcatcaaata tgccgtctga aacagaaaaa tgcgtttcag gcggcatttt
    21481 ttccgtcaat acgaatccgg aatgtgtttg aaatacagca tgaccaaata ccaaaacagt
    21541 gtcgcggcgg ctgagccaag cagtacccat gcgctgattt tggcgacttc gcacagggcg
    21601 cgtttttgcc tgcggctgaa aaggcgcttg ataatcaacg cggcaagtgc gaaaaagaaa
    21661 aaaatacgga aaagtctgcc tatcatgtcg ggaagctggg ttaggaccgt ctgaaaaagt
    21721 atggttcggt tgcggcggca tgggaacgaa tgtttcggcg tgtcaggctg cttgtccgac
    21781 cgtgtcaaaa aatcatgccg tttgcgatat aataacgctt ttcgccgcat ttgttggaac
    21841 aggatatgag tcaaaatgcc tcggtgggca ttgtaacgcc ccaaaaaatt ccgtttgagg
    21901 tgccactggt tttggaaaac ggtaaaactt tgccgcgttt cgatctgatg attgaaacct
    21961 acggcgagct gaatgctgaa aaaaacaatg cggttttaat ctgccacgcg ctgtcgggca
    22021 accatcatgt tgcgggcaaa cattcggcgg aggataaata tacgggctgg tgggacaata
    22081 tggtcggtcc cggaaaaccg attgatacgg aacgtttttt cgtggtcggt ttgaataatc
    22141 tgggcggctg cgacggcagc agcgggcctt tgtcgattaa tcctgaaacg ggcagggaat
    22201 acggcgcgga ttttccggtg gttacggtga aggactgggt aaaatcacaa gccgcgcttg
    22261 ccgattatct cggcatcgaa caatgggcgg cggttatcgg cggcagcttg ggcggcatgc
    22321 aggctttgca gtggacgatt tcctatcccg aacgcgtgcg ccacgccttg gtgattgcgt
    22381 ccgcgccgaa gctttccacg caaaatatcg cgttcaacga tgtggcgcgt caggcgattt
    22441 tgaccgatcc cgatttcaac gaaggacatt accgcagccg caacaccgtt cccgctcggg
    22501 gcttgcggat tgcccgcatg atggggcaca ttacctatct tgccgaagac ggtttgggca
    22561 aaaaattcgg acgcgatttg cgttccaacg gctatcaata cggctatggc gttgaatttg
    22621 aagtagaatc ctatctgcgc tatcaaggcg acaaattcgt cgggcggttt gatgccaaca
    22681 cctatttgct gatgaccaag gctttggact atttcgatcc ggcggcggat ttcggcaaca
    22741 gcctgacccg cgccgtgcag gatgttcagg caaaattctt tgtcgccagc ttcagcaccg
    22801 actggcgttt cgcgcccgaa cgttcgcacg aactggtcaa ggcactgatt gccgcccaaa
    22861 aatccgtgca gtatatcgaa gtcaaatccg cacacgggca cgatgccttt ttaatggaag
    22921 acgaagccta tatgcgtgcg gtcgccgcct atatgaacaa cgtttataag ggatgtcagc
    22981 aatgaacctg cgcgatgatt tgcaactgat ttacgaccgg atacccgaag gcagccgcgt
    23041 cttggacttg ggctgcggcg acggcgaact gcttgccgca ttggtcgaac acaaaaaatg
    23101 cagcggctac ggcatcgaaa tcgacaccga cagcgtgatt gccgcgatgt cgcgcggcgt
    23161 gaatgttatc caagccgatt tggaagaagg tttaaccgca ttcaacgatc aaagttttga
    23221 tgtgattgtg ttgagccaaa ccatccaagc gatgcagaat accgaaaaaa tcctgcgctg
    23281 cctgatgcgc gttgccaaac aggcgattgt cagcttcccg aatttcggct actggcgcaa
    23341 ccgcgtccaa atcgcactcg gcggacatat gccggtttcc gaacgtatgc cctaccattg
    23401 gtatgatacg ccgaacatcc attggtgtac gctcaaagac tttgatttgt tgtgcgccaa
    23461 aaacaacata cgcgtcctcg agcgcgccgt gatgacgggc aacagacagg tcaaacattt
    23521 ccccaacctt ttgggcagcc ttgcgtttta ccgcgtcggg taagacaggt tgagaacctt
    23581 gttggtatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc cgtactattt
    23641 gtactgtctg cggctcgccg ccttgtccta gtttaaattt aatccactat aataacgagg
    23701 ttcttccgtt gtactaacaa gaagccgtct gaaaaatgaa acggtaggtt ttcagacggc
    23761 ctctttaata ttggaaacaa tagaatgaaa aacacaacgt catcacttct cttatggctt
    23821 gccgcaatca tgctggccgc gtgttctccg agcaaaaacg ataaaaccaa agaagtcggt
    23881 gcatccgctg cttcgtcctc cgcgtcatca gcttcttccc aaaccgattt gcaaccggcc
    23941 gcatccgccc ctgataacgt caagcaggca gaaagcgcgc cgccgtcaaa ttgcaccggc
    24001 ctgcaccccg cctccggcat tgacgatctc atgcagcaaa tcgccgaaca catcgactcg
    24061 gactgtctgt ttgccctttc ccatcacgaa ctggaaaccc gtttcggctt acccggcgga
    24121 ggctatgaca acatacagcg gctgctgttt cccgacatcc gccctgaaga tcccgactac
    24181 catcagaaaa tcatactggc aattgaagac ttgcgttacg gaaagcgcac gatcagccgg
    24241 caggcacaag atgccttgat ggaacaggaa cgccgcctcc gagaagcgac gctgttgctg
    24301 atacagggca gtcaagaaac ccgcggacaa ggcgaggagc cgaaacgcac gcgttatttt
    24361 gaagtttcgg caacccctgc ctattcgagc cggcacaaca acggacttgg cggcaatttc
    24421 caatacatcg gccaattgcc cggctatctg aaaatacacg gagaaatgct tgaaaaccaa
    24481 tcactcttcc ggctgtccaa ccgtgaacgc aatcccgaca aaccgttttt agacatccat
    24541 tttgacgaaa atggcaaaat cacgcgtatt gtcgtttacg aaaaaaacat ctacttcaat
    24601 ccaaacttgg ggcgaagata aaaaaatgcc gtctgaaacg ttttcagacg gcatttttca
    24661 atgcaacaaa tcaagcgcgt ttgcggaatt cgccggtgcg ggtatcgatt tcgattttgt
    24721 cgccgttttc gatgtaagac atcacttgga tttcggtgcc cccgaccagg cgcgcggttt
    24781 tcatcacttt gcctgaagta tcgcctttga cggcaggctc ggtatactcg acttcgcgta
    24841 cgatgatggt gggcagttct acggagatgg ggttgccttc gtagaaggta acttcgcatt
    24901 ggtcttccat accgtcaacg atgaatttca acgcgtcgcc gatgttgtcg gcttcgattt
    24961 cgtattggtt gaattcttca tccataaaga cgtacatcgg atcggcaaag tagctgtacg
    25021 tacagttttt gcgggacagg atgaccacgt cgaatttgtc gtcggctttg taaatggttt
    25081 cggaagccgc gccggtcagc aggtttttca gtttcatgct gactttggcg gaagagcggc
    25141 cgcctttgat gtattcggtt ttttgaacga ccataggatc gttgccgacc ataaatacat
    25201 tgccggcgcg cagttcttgt gctgttttca ttgtgatttt ccaatcaaat cggttgtcgt
    25261 aaaacgcgct attctagcgt attttttgat gctttgaaac aaaggcagcg agtttttcag
    25321 gagctgacgg ctgctcgaaa agataacggc tccaatcctc cgcgccttgc cgccagccgt
    25381 tttgatgttg ttgcagggtt tgccaacatt cgaggcgttg cgtggcggat aaagcctctc
    25441 cgccgttgag gtcgtctgaa aggcggcggt gtaccgatgc ggtttcgggc gagtaaaatc
    25501 catgcgcctt atcccaaaag gcgtggagtt tatcgagatg gacattctcg tcttgcgggt
    25561 agatgtgcca aaagaagggt ttgccggcaa gctgggcgcg cacgaaactg tcttcgccgc
    25621 gtatcacggc gcagtcggcg aggtgcagca gtttgtcgaa gtcctgttgc ggcacgaaag
    25681 ggattttgac gaggcggacg gatgccgtct gaaaaacatc gccgtcgttt tgcagggcat
    25741 cttgcggaat aacgccgctt tgtttgaggc tgtcgatgat ttgcgtcccc gccagcaaca
    25801 gtgtcatcgg gctgcctgcc tgtcgccaca tttccagcca ctttgcccaa acatcgctcc
    25861 gatagccgaa aagcagccat tcgggggcgt ttttttcggg aagcatcagc ctcttgcgca
    25921 aggctccgct atcgaaacgg acggcttcgc agtaatcgcg ttcgcgtatc agtccgccgc
    25981 ttttttcgct gaagcccata aaccaaaaat atttttgaac accttcctgc ggcgaaggca
    26041 tcaggtgcag cctttcattg ctttcctccg cgctcaaata ttcccaattc agccaaagcg
    26101 gcttgtgtcg gcggataatg tgcagcacat tttcgggcag gtcgcaggca aaagtttcga
    26161 tgacgacatc gggaacaggc gcggtatcaa tgtctgccgc ttcggcatac caagtgcgga
    26221 catgaatatc ctgatgaacg cagggaacat cgggcaaatc aggacaaagc gcacgcaagg
    26281 cggacacatc gtccgtccac aaatgcacct gccaaccgag ttcgcggtgc aaaacacggg
    26341 caagccgcca cgaaacgccg atgtcgccga aattgtcgat gaccttgcaa aaaatccaac
    26401 agacaaaagg aggagtattc atagaaataa cggaaacggg ctaaaacgcg ccaaaatagt
    26461 ggaacaaata cacaatcagc aaaataatcg gaataaacag caacatagtt tgacgtttca
    26521 aatcaaactc ttgtttcaaa tgatgcttga tggtgtcgaa aaggacgaat ttgaacaaaa
    26581 acgtcgcttc gcaggcaagg atgatgccgt taatcagatg ctggggtttg tcttcgggcg
    26641 gattggcggc gaaaacggca agcgtcgcca agggtatcag gcagacgaca agccagcgga
    26701 caagcaggtt tcgggacatt gcaaggcttt caaaaaagca aaacgccatt atcgccccaa
    26761 gcgcacgcct tgtctaacgg acgcaccgga gatgccgtct gaagccttgc cgcaggaaaa
    26821 caccgcaccg ccgaaccttt aaatacgccc gagacgggcg ttccccgaaa aatcggacgc
    26881 tgccgccgat gcctgaggac aaggcggcag caggcacggg catattgaaa acccggcgcg
    26941 tcggtttgaa
//
LOCUS       DAXIEB010000013        26374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000013 DAXIEB010000000
VERSION     DAXIEB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.013"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(13..1308)
                     /locus_tag="ACFOA3_000485"
     CDS             complement(13..1308)
                     /locus_tag="ACFOA3_000485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6897542.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLQTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="ACFOA3_000486"
     CDS             complement(1434..2321)
                     /locus_tag="ACFOA3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6897543.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="ACFOA3_000487"
     CDS             complement(2321..3286)
                     /locus_tag="ACFOA3_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6897544.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="ACFOA3_000488"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="ACFOA3_000488"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6897545.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="ACFOA3_000489"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="ACFOA3_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="ACFOA3_000490"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="ACFOA3_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6897546.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="ACFOA3_000491"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="ACFOA3_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6897547.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="ACFOA3_000492"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="ACFOA3_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6897548.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDIIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLNSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="ACFOA3_000493"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="ACFOA3_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6897549.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8697..9770
                     /locus_tag="ACFOA3_000494"
     CDS             8697..9770
                     /locus_tag="ACFOA3_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6897550.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDKSG
                     RLNLHEADFGWLTHKIIQTASGCPGKVVSVLEGGYTLESLSKSAAEHIRVLAGLGKSE
                     TAAAYQAELDHNREHVPPDPNPA"
     gene            10025..11089
                     /locus_tag="ACFOA3_000495"
     CDS             10025..11089
                     /locus_tag="ACFOA3_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6897551.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVVWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEDVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            11230..11553
                     /locus_tag="ACFOA3_000496"
     CDS             11230..11553
                     /locus_tag="ACFOA3_000496"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6897552.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            11625..11948
                     /locus_tag="ACFOA3_000497"
     CDS             11625..11948
                     /locus_tag="ACFOA3_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6897553.1"
                     /translation="MDNCIFCKIAAKDILAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            11995..12198
                     /gene="tatA"
                     /locus_tag="ACFOA3_000498"
     CDS             11995..12198
                     /gene="tatA"
                     /locus_tag="ACFOA3_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6897554.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            12202..12888
                     /gene="tatB"
                     /locus_tag="ACFOA3_000499"
     CDS             12202..12888
                     /gene="tatB"
                     /locus_tag="ACFOA3_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6897555.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTVSDGISDVMPSERSYASTETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            12902..13672
                     /gene="tatC"
                     /locus_tag="ACFOA3_000500"
     CDS             12902..13672
                     /gene="tatC"
                     /locus_tag="ACFOA3_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6897556.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            13725..14330
                     /locus_tag="ACFOA3_000501"
     CDS             13725..14330
                     /locus_tag="ACFOA3_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6897557.1"
                     /translation="MNALYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGSIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            14346..14699
                     /locus_tag="ACFOA3_000502"
     CDS             14346..14699
                     /locus_tag="ACFOA3_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6897558.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            14689..16503
                     /locus_tag="ACFOA3_000503"
     CDS             14689..16503
                     /locus_tag="ACFOA3_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6897559.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDIKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            16693..17370
                     /gene="misR"
                     /locus_tag="ACFOA3_000504"
     CDS             16693..17370
                     /gene="misR"
                     /locus_tag="ACFOA3_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6897560.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            17384..18790
                     /gene="misS"
                     /locus_tag="ACFOA3_000505"
     CDS             17384..18790
                     /gene="misS"
                     /locus_tag="ACFOA3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6897561.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SVDGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            18908..21334
                     /locus_tag="ACFOA3_000506"
     CDS             18908..21334
                     /locus_tag="ACFOA3_000506"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6897562.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            21379..21624
                     /gene="rpsP"
                     /locus_tag="ACFOA3_000507"
     CDS             21379..21624
                     /gene="rpsP"
                     /locus_tag="ACFOA3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6897563.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            21640..22149
                     /gene="rimM"
                     /locus_tag="ACFOA3_000508"
     CDS             21640..22149
                     /gene="rimM"
                     /locus_tag="ACFOA3_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6897564.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            22149..22898
                     /gene="trmD"
                     /locus_tag="ACFOA3_000509"
     CDS             22149..22898
                     /gene="trmD"
                     /locus_tag="ACFOA3_000509"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6897565.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            22914..23279
                     /gene="rplS"
                     /locus_tag="ACFOA3_000510"
     CDS             22914..23279
                     /gene="rplS"
                     /locus_tag="ACFOA3_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6897566.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            23600..24325
                     /locus_tag="ACFOA3_000511"
     CDS             23600..24325
                     /locus_tag="ACFOA3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6897567.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINTVQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCNGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            24360..25235
                     /locus_tag="ACFOA3_000512"
     CDS             24360..25235
                     /locus_tag="ACFOA3_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6897568.1"
                     /translation="MNLYDLLLAPFTEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            25312..26232
                     /locus_tag="ACFOA3_000513"
     CDS             25312..26232
                     /locus_tag="ACFOA3_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6897569.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEESGHHHDHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKA
                     DPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEF
                     IAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKL
                     YSDALGSAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         6818 a         7899 c         6071 g         5586 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt gatgcggttc
      661 gatgcgcgtc atcggggact gctcgaaaat ctgcgcgccg gcttcggcag cggcaccggc
      721 aacacccaac gtgtaattga gcggatgcag gtgtccggat aaggggtcga attgtgcccc
      781 ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa gttgataatg
      841 actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt cccaatgctg
      901 cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcctg
     1021 ctccaagccg acctgttttt caatttcccc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gtttgcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcaggtgta ttttcggacg gaatgataca ggctgtcggg ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatt
     1561 aggatggtcg ccaagacatt catctgcgga tcgagaccca acttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gactggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtacgc catacacagc
     1861 gtcgtatgtc cgaggaagat ggtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcagcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa tgccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacagac
     2941 aaattaccgt cgtagtcagc gcagtcttaa ttgaagacca ataagtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gcgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accgcacgct
     3241 gccccggacg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa caacggagtc ggttggtttt ccggccagct gatatagacg gtttcgtccc
     3361 aagtcggcgg tgtaatgttg cgcacatacc agtaaggggc ggggacttgg cttttgacga
     3421 cgcgcccgtt gccgagcttg atatggtaaa tggcgaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagtgt agattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggattttgag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agttaaacgt ttgatgccgt ctgaaactct tgtaaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tctttggggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg gaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccga tggtcaacgc catagaaaag ccgtgcaagg ccgcaccgcc
     5461 gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcaaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccaac ttgcgggccg ataaattcga cttggcgcaa
     5881 agtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt gccgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cggctttcgg cttccacacc gaaccgatgg aaatattctg
     6301 caatttgcgt ctgcgtccat accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggcgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgcca aaaatgctgc cacccgcgct gttcaagctc agactgacag caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtctg cacctgactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagtgc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caagtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgatgtcgcg cgctttaagc tgcgtttctg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ctgcgttttt
     7921 cagcgcggca tccactttgg attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaccccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggctttcc
     8281 agatgcggct tgtcggcaca aatcaatcga cttttgcggc aatcgcattg cgttctactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cactattgcg cctctttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gcacagggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgcg cttgtccact
     8941 cgagcaaata tctgaaccgt ttggaatctt gcctgcccca aaaaggcaag attttccgcc
     9001 tggataacga cactgcaatc agcacaggct cgctgtctgc cgcacacttt gccgccggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgtaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggcgtcatg catgccattg ccgaataccg cctgaaacgc attgccgtca
     9241 tcgatttcga tgtccactac ggcgacggta cggcagaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc tttttccttt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcacattcc
     9421 gcgaagccgt ccgcagacag tggctgcccc ggcttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tggacaaatc gggcaggctc aacctgcacg
     9541 aggcggattt cggctggctg acacacaaaa ttattcaaac ggcatcgggc tgcccgggta
     9601 aggtcgtatc cgtgctggaa ggcggttata cccttgaatc cttatcaaaa tccgccgccg
     9661 aacacatccg cgtgctggca ggattgggaa aatccgaaac ggccgccgcc tatcaggcgg
     9721 aattggacca caacagggaa cacgtcccgc ccgatccgaa cccggcataa gcacgccgga
     9781 ccggccgttt taaaacaaag gctatgacgg cataggtatt atttaaatcc gattcggttt
     9841 gcaggcggta tcattatttg acctgaagca aaacaagaaa cttttatctg cttgaaatag
     9901 gttttttgta gccaaataac acttgggcag attgaatccg gctcttaccg gccgccgttt
     9961 ccatcccaac cgccgtccga catttttcag acggcattcc tacttgaaaa caaggagcgt
    10021 tacaatgaaa gcagcacgtt tttacgacaa aggcgacatc cgcatcgaag acatccccga
    10081 accgaccgtc gcccccggca ccgtcggcat caatgttgtc tggtgcggca tctgcggtac
    10141 cgacctgcac gaattcatgg aaggcccgat tttcattccg ccttgcggtc atccgcaccc
    10201 gatttccggc gagtccgcac ccgtcacgat gggacacgag ttctccggcg tggtctatgc
    10261 cgtcggcgaa gacgtggacg acatcaaagt cggccaacac gtcgtggtcg agccttacat
    10321 cgtacacgac gacgtaccga ccggcccggg cagcaactac cacctctcta aagacatgaa
    10381 cttcatcggc ttgggcggct gcggaggtgg cttgtctgaa aaaatcgctg tcaaacgccg
    10441 ttgggtgcat ccgatttccg acaaaatccc gttggatcaa gccgctttga tcgaaccgct
    10501 gtctgtcgga caccacgcct atgtacgcag cggcgcgaaa gcaggcgacg tcgcattggt
    10561 cggcggcgca ggtccgatcg gtttgctgtt ggctgccgtg ttgaaagcca aaggcatcaa
    10621 agtcatcatc accgagttga gtaaagcacg caaagacaaa gcgcgcgaat ccggcgttgc
    10681 cgactacatc ctcgacccgt ccgaagtcga tgttgtcgaa gaagtgaaaa aactgaccaa
    10741 cggcgaaggc gtggacgtgg cattcgaatg taccagcgtg aacaaagtgt tggatacttt
    10801 ggtcgaagcc tgcaaacctg ccgccaattt ggttatcgta tccatctgga gccaccctgc
    10861 aaccgtcaac gtccacagcg tcgtgatgaa agaactggat gtgcgcggca cgattgccta
    10921 ctgtaacgac cacgccgaaa ccatcaaact ggtcgaagaa ggcaaaatca acctcgagcc
    10981 tttcatcacc cagcgcatca agctggacga actggtttcc gaaggcttcg agcgtctaat
    11041 ccacaacaac gaatccgccg ttaaaattat tgtgagtcca aacctgtaag caaattacat
    11101 ttgcaacagc acaaatgccg tctgaaacgc tcagacggca ttttccaacg aaacgcaaca
    11161 ggcgtattgc ccgagagcag ccgtttagcc tatcattccg aacaaacaga aacaaacaga
    11221 aggaaaatca tgggagattc cgtactatcc gccatccaac aaaccatcac ccagcgaaaa
    11281 tctgccaatc cgtccgaatc ttacgtcgca cagctcttgc ataagggcga agacaaaatc
    11341 ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga caaaaacccg
    11401 tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct tctgacacac
    11461 cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgccaagg tttgtcgggc
    11521 ttggccgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg cactttccca
    11581 cattcaatcc gtctgaccgc ttttcagacg gcatcggagc cgttatggac aactgtattt
    11641 tctgcaaaat cgccgccaaa gacattctgg cgcaaaccgt ctatgaagac ggcgaaatgg
    11701 tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt cccaaagtcc
    11761 atttcgattc gttggcacac gccgcgcccg aacatcagcc ccttttggga aaaatgatgc
    11821 tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa accctgatca
    11881 acaccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc atgggcacac
    11941 ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata catcatgggc
    12001 agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat attcggcacc
    12061 aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt caaacagggg
    12121 ctgaacgaag gcacagacgg caaagaagcc caaaaagacg atgtaatcga acacaaaaaa
    12181 gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt ttttgtcggc
    12241 attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg caccgccgga
    12301 cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt tgacactcaa
    12361 atcgaactgg aagaactaag gaaggcaaag caggaattcg aagctgccgc tgctcaggtt
    12421 cgagacagcc tcaaagaaac cggtacggat atgcagaaca gtctgcacga tatttccgac
    12481 ggtctgaagc cttgggaaaa actgcccgaa cagcgcacgc ctgctgattt cggtgtcgat
    12541 gaaaacggca atccccttcc cgatgcggca aacaccgtat cagacggcat ttccgacgtt
    12601 atgccgtccg aacgttccta cgcttccacc gaaacccttg gggacagcgg gcaaaccggc
    12661 agtacagccg aacccgcgga aaccgaccaa gaccgtgcat ggcgggaata cctgactgct
    12721 tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac cgctgttgaa
    12781 acccctgttc cgcataccac ttcgctgcgt aaacaggcaa taagccgcaa acgcgatttg
    12841 cgtcccaaat cccgcgccaa acctaaattg cgcgtccgta aatcataaag agagcaatcc
    12901 ggtgtccgaa acacaaaacg aacaacccgt ccaaccgctt gtcgagcatc tcatcgagct
    12961 gcgccgccgc ctgatgtgga cggttgtcgg cattctcgtc tgctttttcg gcctaatgcc
    13021 gtttgcccaa caactctata cttttatcgc cgacccgctg atggcaaacc tgcccaaaga
    13081 caccagcatg attgccaccg atgtcatcgc accgtttttc gtgccggtca aagttaccct
    13141 gatggcggca tttttgattt cgctgccgca tacgctctac caaatctggg cattcgtcgc
    13201 gcccgcactc taccaaaacg aaaaacgcct gattacgccg ctcgtcctct ccagcgtcag
    13261 cctgtttttc atcggcatgg catttgccta ctttctggtt ttccccgtca ttttcaaatt
    13321 ccttgccagc gttacccctg tcggtgtcaa tatggcgaca gacatcgaca aatacctatc
    13381 ttttatctta ggcatgtttg ttgcgttcgg cacaacgttt gaagttccca tcgtcgttat
    13441 cctgttaacc aaaatcagcg tggtaacaac cggacagctc aaacgcgccc gcccctatgt
    13501 gattgtcggc gcgtttgtca ttgccgccat catcacgccg cccgatgtga tttcacaaac
    13561 cctgcttgcc attccgctga ttctcttata cgaagcaggt atttggttcg gacgcttttt
    13621 cacgccacgt tcagaacagg atggcgacat acagccgcct gcaacaacct gacactatgc
    13681 cgtccgaacc tccgcctcat accgcaacag attaaggaat acctttgaat gccctctatt
    13741 taggttcaaa cagcccgcgc cgaatggaaa tcctgacaca gttgggctat caggtcgtca
    13801 agctgcctgc cgacatcaat gaaacggtca gacagaacga agaccctgcc cgttacgttc
    13861 aaaggatggc agaagaaaaa aaccgaaccg ccctgaccct cttttgcgaa accaacggca
    13921 caatgcccga tttccccctg attaccgccg acacctgcgt cgtttcagac ggcatcatat
    13981 taggcaaacc ccactcccaa gccgaagcaa tcgaattttt aaaccgattg tccggcaaac
    14041 aacataccgt cctgactgcc gtctgcattc attatcgcgg caatgcggaa aaccgcgtcc
    14101 aaaccaaccg cgtcgttttc aagcccctga gttcggaaga aatttccgcc tatgtccaaa
    14161 gcggcgaacc gatggacaaa gccggtgcct acgccgtaca aggcataggc agcatcttta
    14221 tccaatctat cgaaggcagc ttcagcggca ttatggggct gcccgtttat gaaaccgttt
    14281 cgatgttgca ggatttggga taccgtaccc cacccttcat ccgagcctaa cccgtaaaga
    14341 ccgccgtgaa cagacaaacc gcttacctcc ttgcctcttt cagcctgatc gcactgataa
    14401 tcctgtccct ttcctgggaa ctgtggattg caccgttgcg cccgggcggc tcgtggctcg
    14461 cgctcaaagc cctccccctc tgtctgccgc tttcaggcat cttgaaaaag aaaatctata
    14521 cttaccaata cagctccatg ctggttctga tttactttgc cgaagccgtc atgcgcctgt
    14581 tcgacgccta tcccgcagaa cagatttgcg ccgcgctttc cgcagtattc agcatcatct
    14641 tcttcatatc ctgcctgtcg ttcgtcaaac aatacaagga aacaaacaat gcccgctgaa
    14701 acgaccgtat ccggcgcgca ccccgccgcc aaactgccga tttacatcct gccctgcttc
    14761 ctttggatag gcatcgtccc ctttaccttc gcgctcaaac tgaaaccgtc gcccgacttt
    14821 taccacgatg ccgccgccgc agccggcctg attgtcctgt tgttcctcac ggcaggaaaa
    14881 aaactgtttg atatcaaaat ccccgccatc agcttccttc tgtttgcaat ggcggcgttt
    14941 tggtatcttc aggcacgcct gatgaacctg atttaccccg gtatgaacga catcgtctct
    15001 tggattttca tcttgctcgc cgtcagcgcg tgggcctgcc ggagcttggt cgcacacttc
    15061 ggacaagaac gcatcgtgac cctgtttgcc tggtcgctgc ttatcggctc cctgcttcaa
    15121 tcctgcatcg tcgtcatcca gtttgccggc tgggaagaca cccctctgtt tcaaaacatc
    15181 atcgtttaca gcgggcaagg cgtaatcgga cacatcgggc agcgcaacaa cctcggacac
    15241 tacctcatgt ggggcatact cgcctccgcc tacctcaacg gacaacgaaa aatccccgcc
    15301 gccctcggcg taatctgcct gattatgcag accgccgttt taggtttggt caactcgcgc
    15361 accatcttga cctacatagc cgccatcgcc ctcatccttc ccttctggta tttccgttcg
    15421 gacaaatcca acaggcggac gatgctcggc atagccgcag ccgtattcct taccgcgctg
    15481 ttccaatttt ccatgaacac cattctggaa acctttactg gcatccgcta cgaaactgcc
    15541 gtcgaacgcg tcgccaacgg cggtttcaca gacttgccgc gccaaatcga atggaataaa
    15601 gcccttgccg ccttccagtc cgccccgata ttcgggcacg gctggaacag ttttgcccaa
    15661 caaaccttcc tcatcaatgc cgaacagcac aacatatacg acaacctcct cagcaacttg
    15721 ttcacccatt cccacaacat cgtcctccaa ctccttgcag agatgggaat cagcggcacg
    15781 cttctggttg ccgcaaccct gctgacgggc attgccgggc tgcttaaacg ccccctgacc
    15841 cccgcatcgc ttttcctaat ctgcacgctt gccgtcagta tgtgccacag tatgctcgaa
    15901 tatcctttgt ggtatgtcta tttcctcatc cctttcggac tgatgctctt cctgtccccc
    15961 gcagaggctt cagacggcat cgccttcaaa aaagccgcca atctcggcat actgaccgcc
    16021 tccgccgcca tattcgcagg attgttgcac ttggactgga catacacccg gctggttaac
    16081 gccttttccc ccgccactga cgacagtgcc aaaaccctca accggaaaat caacgagttg
    16141 cgctatattt ccgcaaacag tccgatgctg tccttttatg ccgacttctc cctcgtaaac
    16201 ttcgccctgc cggaataccc cgaaacccag acttgggcgg aagaagcaac cctcaaatca
    16261 ctaaaatacc gcccccactc cgccacctac cgcatcgccc tctacctgat gcggcaaggc
    16321 aaagttgcag aagcaaaaca atggatgcgg gcgacacagt cctattaccc ctacctgatg
    16381 ccccgatacg ccgacgaaat ccgcaaactg cccgtatggg cgccgctgct acccgaactg
    16441 ctcaaagact gcaaagcctt cgccgccgcg cccggtcatc cggaagcaaa accctgcaaa
    16501 tgaccctcgc cggcggatgc ggataccgcc cgaaatgtaa agctccatgc aagacattgc
    16561 aaaaaacagc aaaccggtag ggaaatacgc tatcaaaaaa cattgcagcc gtgttaagat
    16621 aaaccgtcaa acaatctttt cacgccccgc ccgaaacagg gtcggggcat acccttacga
    16681 aaaggaaaca ccatgagccg cgtattactc gtagatgacg atgccctgct aaccgaactg
    16741 ctgaccgaat acctgagcgc cgaaggtctg aacgtccgca gcgttcccga cggggaagca
    16801 ggcgtacagg aaatcctgag cgggcagtac gatgtagtcg tattggattc catgatgccc
    16861 aaaatgaacg gcttggatgt cttgaaaaac gtacgcgccc gaagcaccgt ccccatcatc
    16921 atgctgaccg ccaaaggcga cgacatcgac cgaatcatcg gcttggaaat gggcgcggac
    16981 gactatgtcc ccaaaccctg cacaccacgc gaactcttgg cacgcatcaa tgccatcctg
    17041 cgccgcgcac aacacagcgg cgaacagaac aacgcaccca acagcatctc cgtcagcgat
    17101 gtcgtcctgt accccgccaa acgccaggca tccgtcaaag acatgccgct cgaactgacc
    17161 agcaccgaat tcaacctgct cgaagtcctg atgcgccatg ccggacaggt agtcagcaaa
    17221 gaaaccctgt ccgtcgaagc actcgaccgc aagctggcaa aattcgaccg cagtatcgac
    17281 gtacacatct ccagcatccg ccacaagttg ggcgatgcct ctctgattca aaccgtacgc
    17341 ggcttgggct acctgtttgt caaaaactga aataaacaga taaatgaaac tgttccaacg
    17401 catcttcgcc acattttgcg cggttatcgt ctgtgcaatc tttgtggcga gtttttcttt
    17461 ctggctggtg cagaacaccc ttgccgaaaa ccagttcaac caacgccgca ccatcgaaac
    17521 cactttgatg ggcagcatca tttccgcatt ccgggcacgc ggggacgcgg gcgcgcgcga
    17581 aatcctgacg gaatggaaag acagccccgt ctcatcgggc gtgtacgtta tacagggcga
    17641 cgagaaaaaa gatatcctga accggtatat cgacagctat accatcgaac gcgcccggct
    17701 tttcgccgcc ggacacccgc attccaacct cgtccatatc gaatacgacc gcttcggcga
    17761 agaatacctg ttcttcacca aagactggga caaactccaa gcccgccgcc tgcccagccc
    17821 cctgttgatc cccggcctgc cgctcgcccc gatttggcac gaactcatca tattgtcctt
    17881 catcatcatc gtcggactgc tgatggcata tatcctcgcc ggcaacattg ccaaacccat
    17941 cagaatctta ggcaacggca tggacagggt ggcaaacgga gaacttgaaa cccgtatctc
    18001 ccaacaggtc gacgaccgcg acgacgaatt gtcccatctt gccatccaat tcgacaaaat
    18061 ggtggaaaaa ctcgaaaaac tcgttgccaa agaacgccac ctgctccatc acgtctccca
    18121 tgaaatgcgt tctccccttg cgcgcatgca ggcaattgtc ggactgattc aggcgcagcc
    18181 ccaaaaacag gagcaatatc tcaaacggct ggaaggcgaa ctgacccgca tggatacgct
    18241 ggccggggaa ctgttaaccc tgtcccgtct cgaaacttcc aatatggctt tggaaaaaga
    18301 aagcctgaaa ctcctgccct tcctgggcaa cctggtagaa gacaatcaaa gcattgccca
    18361 gaaaaacgga caaacggtta ccctgtccgt cgacggaaaa atccccgaaa acacaaccat
    18421 ccttgccaac gaaagctacc tgtaccgcgc cttcgacaac gtcattcgca acgccgtcaa
    18481 ctacagtccc gaaggcagca ccatcctgat caacatcgga caagaccaca aacactggat
    18541 aatcgacgtt accgacaacg gccccggcgt ggacgaaatg cagctcccgc acatcttcac
    18601 cgctttctac cgtgcagact ccagtgcgaa caaacccgga acaggactgg ggcttgcatt
    18661 gacccaacat attattgaac agcactgcgg aaaaatcatc gccgaaaaca tcaaaccgaa
    18721 cggtctgcgg atgcgcttca tcctgcccaa gaaaaaaacc ggttccaaaa cagaaaaaag
    18781 tgcgaactga ccataatgcc gtctgaaccg gcttcagacg gcattgcaca aacagttacc
    18841 ccaaaacaaa cacacagcgg gtgcatctcc aacaatcacg cacataccgt ccgcaaagga
    18901 atatatcatg tcggcacaaa ccgatccggg ctactttttc atgcccaacc acattattct
    18961 gataggcgcg agcgaacgtc cgtacagcct gggcgaacgc gtactcagca acctgctgag
    19021 tacgcccttt caaggaaaaa tcacccccgt aaacccgcgc caccacacca tagccgggct
    19081 tcccgcctac accagcctca acaaaatccc cggcaatgca gacctgatta ttgccgttac
    19141 cctacccgac agttacgaca ccctcttcaa aacctgccgc aaaaagcagc tccgacacat
    19201 catcctcata caggactggg acagcctgtc tgccgcagaa ctgcacaccg ccgaaactgc
    19261 catccgcaaa caccacggca acggactcaa catcaccgcc tgcaccaccg caggcatcca
    19321 actgccctca ctcggactca acatcagtac ccaagacgga tacgccgcag gccataccgc
    19381 catactgacc ggcaatgccg ccgtcagccg tcaaatcgac aacatcctga acaaactccg
    19441 tcaaggcaca tcccgccaca tcagcctgca tcccggcatc agccccatca catccgccga
    19501 ttggctcaac cgcttcggac acagcctgca caccaaaacc gccgtcctac accacaaccc
    19561 tgaagaggat cagcgcaaac tgttcagcgc aatccgccaa tttacccgcc atacgccgct
    19621 gattctccac atcacctgcc gcacgacaga aaccgaccgt gccgtactgc actgcctcgc
    19681 ccgacactgc aacttcctca tcagtttcaa cgccgacgac ctcgaagccg cactgcgcgc
    19741 ccaactgtcc gaccttccac ccctgtcccg actcgacatc ctgtccgaca cgcctgccga
    19801 atggctgaac gcgcacgcgc caaaaaccct cgccctccac tttcccaacc ttccccatca
    19861 catccgcaac ggacacctga ccggcacacc cacaccctca atctgccacg acatcgcctc
    19921 acgtcagctt gcccgccccg acacccaagc cgtcctaacc atcctcagtc cctccggaca
    19981 cgaggattac aaaaaaacag cacgcgccct tatccgcctg tccgaacaga ccgccaaacc
    20041 cctgctcgtc agcagcccct tttcagacgg cataacacat ttcgacaccc ccactcaggc
    20101 aatccgcacc ctttcctacc gcaacaccgc cgccgccctg aaacaggcac agctcgacat
    20161 tgcaccgccg cagccatgcc gtctgaaaac accgcaaccc caaaacatca aaaaagccct
    20221 tgcagcggca aacccctccc tgctcgccga agccctgcac ctccccccct accggcacac
    20281 cacccataac gccgtacaat tccaattcga cagccacccc ctctatggcg acatcctgac
    20341 cgcacgctgc aacggacaaa ccactgccgt actcccgccg tttaccacgc tcgacagccg
    20401 ccaccttgcc cgctttgccg aactcgacgg cacacaaacc ctcgaccagt tcctgcacac
    20461 actgaccatc attcccgaat accgccaaca cattctcggc atcaccctca acctaaacgg
    20521 cggacaatac agcagcgatt tcatcttaaa gacacccgaa acacacgaca cgcccaaacg
    20581 caagaacaca ggcaaagccg cccaaaccct cgaacatgcc gccgcaaaaa tgcagagtgc
    20641 cgccgcatac ctgaaacaca aaaacccgac agccgccgaa tttctccgcc acacaagcga
    20701 agccgccgca gaactgctcg gcagcaaaac cgaaaccgga gcagccgtac ccaacgtact
    20761 tgccccctat cccgcagcat accccaaaac actgtcccta agaaacaaca cgaccgttac
    20821 cattaccccc attttgcccg aagacgcaga agccaaacag cagttcgtcc gcagcctcgg
    20881 tcccgaagca cggtacacac gcttcatgac ccacaccaac gaactgcccg cagccacgtt
    20941 ggcacgcctg tgcaaccccg attaccactg tgaagccgca tggacggcaa aggatgccga
    21001 cagcaacatc gtcgccgtcg tccgccacag ccgcctgaat cgcaacgaat gcgaattcgg
    21061 catcacactg gcggaacata tgcgcggcag cggactggca cagaaaatga tggaactcat
    21121 catccaaacc gccgcacagc aaggctaccg gactatgagt gccgacattc tcaaaaccaa
    21181 tacccccatg atcaaacttg ctgaaaaatc aggatttacc ctcaaggaat cggacaccga
    21241 aaaaaacctg taccgcgcat atctgaacct tgcggcagac aaaacaacag aaaaaacaaa
    21301 taaaaacttg cgcaccgacc acaaaataac ctaaaatcgg cagtttccat atatccgatt
    21361 ttctcaaaag gactcaaaat ggtagttatc cgtttggcac gcggcggctc gaaacaccgc
    21421 cccttctaca acgtcatcgt tactgactca cgcagccgcc gcgacggccg cttcatcgaa
    21481 cgcgtaggct tctacaaccc cgtagccaac gaaaaacaag agcgcgtccg cctcaatgca
    21541 gaccgcctga accactggat tgcacaaggc gcgcaagtca gcgactccgt tgcaaaactg
    21601 attaaagaac aaaaagccgc ctaatccgca tttgccgcca tgacagacac tcaaaaccgg
    21661 gtagccatgg gctacatcaa aggcgtattc ggcataaaag gctggttgaa aattgccgcc
    21721 aacaccgaat attccgacag ccttttggac taccccgagt ggcatttggt caaggacggc
    21781 aaaaccatca gcgttaccct tgaagccgga aaagtcgtca acggcgaact ccaagtcaaa
    21841 ttcgaaggca taaacgaccg cgacttggca ttctcattgc gcggttacac catcgaaata
    21901 ccccgtgaag cattcgcccc gacagaagaa gacgaatact actggacaga cttggtcggc
    21961 atgaccgtcg tcaacaaaga ccataccgtt ttaggcaagg taagcaacct gatggaaacc
    22021 ggcgcaaacg acgtattgat gattgacgga gaacacgggc agattctgat tccgttcgtt
    22081 tcccaatata tcgaaaccgt cgataccggc agcaagatta ttactgccga ctggggtttg
    22141 gactactgat gcttatccag gcagttacca ttttccccga aatgttcgac agcattaccc
    22201 gctacggcgt aacgggacgc gcgaacagac agggaatctg gcagtttgaa gcagtcaatc
    22261 cccgaaagtt tgccgacaac agattgggct acatcgacga ccgcccgttc ggcggcggcc
    22321 cgggaatgat tatgatggct ccgccgcttc atgcagcaat agaacacgcc aaagcacaat
    22381 cttcccaagc tgcaaaagtc atttacctca gcccccaagg aaaaccgctg acacacccaa
    22441 aagcggcaga actggcagaa cttccgcatc tgattctgct gtgcggacgc tatgagggaa
    22501 tagacgaaag actgctgcaa accagcgtcg atgaagaaat cagcatcgga gacttcgttg
    22561 tttccggcgg agagcttccc gccatgatgc tgatggatgc ggtattgagg ctcgtacccg
    22621 gcgtattggg cgatatgcag tctgccgaac aagattcgtt ctcaagcggc attttggact
    22681 gcccccacta caccaaaccc ttagaatttc aaggcatggc tgttccggaa gtattacgtt
    22741 ccggcaatca tggcttgata gcggaatggc ggttggaaca atcgctgcgc cgcaccttgg
    22801 agcgcagacc cgatcttttg gaaaagcgcg ttttaatccc aaaggaatcc cgcctcttag
    22861 aaaccatccg gcaagagcaa cgggaaatcc aatcataatt taggaaaaaa acaatgaacc
    22921 tgattcaaca gctcgagcaa gaagaaattg cccgcctgaa taaagaaatc cccgaattcg
    22981 caccgggcga caccgtagtc gtatccgtac gcgtcgtgga aggtacccgc agccgtctgc
    23041 aagcctacga aggcgtggtt attgcccgtc gcaaccgtgg tctgaacagc aacttcatcg
    23101 tccgcaaaat ctccagcggc gaaggtgttg aacgtacttt ccaactgtac tctccgaccg
    23161 tcgaaaaaat cgaagtcaaa cgccgtggcg acgtacgccg tgccaaactg tactacctgc
    23221 gcggcctgac cggtaaagct gcacgcatca aagaaaaact gcctgcacgc aaaggttgat
    23281 tcaaaccgtt ttcccccaat gccgtctgaa ccttcagacg gcatttctta ttgctgcgat
    23341 gccggcaagt caacgatatg cctgcccctc atccaccttg attaacaatc actcccgcct
    23401 attcaagcat aatttacaga ccaaacgtta tacagtatca tatcagccat ataacgataa
    23461 acgctttacc gctttccctt tcaaaaccgc aaatcatcat gacaccttcc cttttactat
    23521 caggattgac cttccgcctc atccttgccc tgattgccgt atccctttta tggggcgttt
    23581 acttttgggc agtatccgca tgagcatcat tgtcgaaaac ctgaccgtca gctaccgccg
    23641 ccgacctgcc gtgcaccatg tggacattac ttttgaagaa catagtatgt gggcggtttt
    23701 cggtcccaac ggcgcaggga aatccacctt tctcaaatcc ttgatgggat tgcagcctat
    23761 cgatacaggc agcatccggc tggacggatt gacccgtcag aacattgcct accttcccca
    23821 gcagtccgac atcgaccgct cgcagcctat gaccgttttc gacttggcgg caatggggct
    23881 atggtatgaa atcggttttt tcagaggaat aaataccgtt caaaaacaac gcgttcacga
    23941 agccttggag cgtgtaggaa tgcggcagtt tgcccgccgc caaatcgccc acctctcaaa
    24001 cggacaattt cagcgtgtcc tttttgcccg aatgctggtt caaaacgcca aattcctact
    24061 tctggacgaa cccttcaatg ccgttgatgc acggacaacc tacgagcttc tcgacgtatt
    24121 gcaaaaatgt cattgcaacg gacacgctat cattgccgtt ttgcacgatt acgaacaagt
    24181 ccgcgcctac tttcccaata ccctgctgct cgcccgagaa aaaattgcgg caggcgcaac
    24241 cgagaccatt ctgacagaac cctacctcgc ccaagccaac gccaaaatgc agcaacagga
    24301 aagccccgac tggtgcgcct cataaatact gcccgaaacc acgccccaca ccagacacca
    24361 tgaatctcta cgacctgctc cttgccccct ttacagaatt cgactttatg cgctacgccc
    24421 tcgcatccgt cttctgcctg tccctcagtg ccgcacccgt cggcgtattc ctcgtcatgc
    24481 gccgtatgag cctgataggc gacgcattga gccacgccgt cctgcccggt gccgccgtcg
    24541 gctacatgtt tgccggcttg agcctgcccg ccatgggttt gggcggcgta gccgcaggca
    24601 tgctgatggc actgcttgcc ggactcgtca gccgcttcac caccctgaaa gaagatgcca
    24661 actttgccgc cttttatctc agcagcctcg ccatcggtgt agtcctcgtc agcaaaaacg
    24721 gcagcagcgt cgatttgctc cacctccttt tcggctccgt acttgccgtc gatattcctg
    24781 ccctgcaact catcgccgcc gtatccaccc tcacactgct tacccttgcc gtcatctacc
    24841 gcccgctcgt actcgaaagc atcgaccccc tgttcctcaa atccgtcggc ggcaaaggcg
    24901 ggctttggca cgtcctcttt ctcgtcctgg tcgtcatgaa cctcgtatcc ggctttcaag
    24961 ccctcggcac actcatgtcc gtcggactca tgatgctgcc agccattacc gcccgcctat
    25021 gggcgaagca catgggcgca ctcatcctcc tatccgttct gacagccctg ctgtgcggct
    25081 tgagcggact gcttatttcc taccacatcg aaattccctc cggtcccgcc atcatcctct
    25141 gttgcagcgt cctttatctc ttttccgtca tactcggcaa agaaggcggc attctgacca
    25201 aatggctcaa aaaccaccgc caccacacca cctgaccccg caagccgctt gaacaaacag
    25261 gcggcttgtt ttaccataac agatgttacg ttatatcaca ggagaacagt tatgaaacac
    25321 ctcaaactca cccttattgc cgcattgcta accaccgccg caactgctgc ccccctgccc
    25381 gtcgtaacca gcttcagcat tttaggcgac gttgccaagc aaatcggcgg agagcgcgta
    25441 tccatacaaa gtttggtcgg agccaaccaa gatacgcacg cctatcatat gaccagcggc
    25501 gacattaaaa aaatccgcag tgcaaaactc gttctgctca acggcttagg acttgaagcc
    25561 gccgacatcc aacgcgccgt caaacagagc aaagtatcct atgccgaagc gactaaaggc
    25621 atccaacccc tcaaagccga agaagaaagc ggacaccatc acgaccacga tcatgaccat
    25681 gaccacgaag gacaccacca cgaccacggc gaatatgacc cccacgtctg gaacgacccc
    25741 gtccttatgt ccgcctatgc ccaaaacgtt gccgaagccc tgataaaggc cgaccccgaa
    25801 ggcaaagttt attatcaaca acgcttgggc aactaccaaa tgcagctcaa aaaactgcac
    25861 agcgacgcac aagccgcatt taatgccgtc cctgccgcca aacgcaaagt cctgaccggg
    25921 cacgatgcct tttcctatat gggcaaacgt taccatatcg aattcatcgc cccgcaaggc
    25981 gtgagcagcg aagccgagcc ttcagccaaa caagtcgccg ccatcatccg gcaaatcaaa
    26041 cgcgaaggca tcaaagccgt ctttaccgaa aacatcaaag acacccgcat ggttgaccgc
    26101 atcgccaaag agaccggtgt caacgtcagc ggaaaactgt attccgacgc actcggcagc
    26161 gcgcccgccg acacctacat cggaatgtac cgccacaaca tcaaagcctt aaccaacgcg
    26221 atgaagcaat aaaaccgttt gattggtttg aatttgaaaa ggcaaaaggc cgtctgaaaa
    26281 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acagatagta
    26341 cggtaaggcg aggcaacgct gtactggttt ttgt
//
LOCUS       DAXIEB010000014        26204 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000014 DAXIEB010000000
VERSION     DAXIEB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.014"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            627..2075
                     /gene="gnd"
                     /locus_tag="ACFOA3_000514"
     CDS             627..2075
                     /gene="gnd"
                     /locus_tag="ACFOA3_000514"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6897570.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPLGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVRMGGLFARQALPVGGDKKQWVGDLRQALLASKIISYA
                     QGFMLIREAGESYGWDLGYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2138..3409
                     /gene="waaA"
                     /locus_tag="ACFOA3_000515"
     CDS             2138..3409
                     /gene="waaA"
                     /locus_tag="ACFOA3_000515"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6897571.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3450..3899
                     /locus_tag="ACFOA3_000516"
     CDS             3450..3899
                     /locus_tag="ACFOA3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6897572.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3932..4906
                     /locus_tag="ACFOA3_000517"
     CDS             3932..4906
                     /locus_tag="ACFOA3_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6897573.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            4975..5050
                     /locus_tag="ACFOA3_000518"
     tRNA            4975..5050
                     /locus_tag="ACFOA3_000518"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5008..5010,aa:Lys,seq:ttt)
     gene            5269..6522
                     /gene="murA"
                     /locus_tag="ACFOA3_000519"
     CDS             5269..6522
                     /gene="murA"
                     /locus_tag="ACFOA3_000519"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6897574.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGARIERVSG"
     gene            6603..7781
                     /locus_tag="ACFOA3_000520"
     CDS             6603..7781
                     /locus_tag="ACFOA3_000520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6897575.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQVAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7847..8095)
                     /locus_tag="ACFOA3_000521"
     CDS             complement(7847..8095)
                     /locus_tag="ACFOA3_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6897576.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(8187..9104)
                     /gene="ftsX"
                     /locus_tag="ACFOA3_000522"
     CDS             complement(8187..9104)
                     /gene="ftsX"
                     /locus_tag="ACFOA3_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6897577.1"
                     /translation="MSIIHYVSLHVESARTALKQLMRQPVGTILTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9101..9751)
                     /gene="ftsE"
                     /locus_tag="ACFOA3_000523"
     CDS             complement(9101..9751)
                     /gene="ftsE"
                     /locus_tag="ACFOA3_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6897578.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9946..10428)
                     /locus_tag="ACFOA3_000524"
     CDS             complement(9946..10428)
                     /locus_tag="ACFOA3_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6897579.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEANEKSLTEAVKLAHSKCR
                     "
     gene            10552..10905
                     /gene="arsC"
                     /locus_tag="ACFOA3_000525"
     CDS             10552..10905
                     /gene="arsC"
                     /locus_tag="ACFOA3_000525"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6897580.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            10902..11558
                     /locus_tag="ACFOA3_000526"
     CDS             10902..11558
                     /locus_tag="ACFOA3_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6897581.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRIKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFNWLFH
                     "
     gene            11751..13145
                     /gene="gltX"
                     /locus_tag="ACFOA3_000527"
     CDS             11751..13145
                     /gene="gltX"
                     /locus_tag="ACFOA3_000527"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6897582.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGAAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEAIDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAECLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13166..13561
                     /locus_tag="ACFOA3_000528"
     CDS             13166..13561
                     /locus_tag="ACFOA3_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897583.1"
                     /translation="MPSDVGMRLQTVFKRKLNMKKIFYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13565..14056
                     /locus_tag="ACFOA3_000529"
     CDS             13565..14056
                     /locus_tag="ACFOA3_000529"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6897584.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWYGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14180..16774)
                     /gene="mutS"
                     /locus_tag="ACFOA3_000530"
     CDS             complement(14180..16774)
                     /gene="mutS"
                     /locus_tag="ACFOA3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6897585.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPETHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELEDQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17218..18222
                     /gene="gap"
                     /locus_tag="ACFOA3_000531"
     CDS             17218..18222
                     /gene="gap"
                     /locus_tag="ACFOA3_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6897586.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18292..18411)
                     /locus_tag="ACFOA3_000532"
                     /pseudo
     CDS             complement(18292..18411)
                     /locus_tag="ACFOA3_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18480..19160)
                     /gene="tsaA"
                     /locus_tag="ACFOA3_000533"
     CDS             complement(18480..19160)
                     /gene="tsaA"
                     /locus_tag="ACFOA3_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6897587.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19275..19910
                     /locus_tag="ACFOA3_000534"
     CDS             19275..19910
                     /locus_tag="ACFOA3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6897588.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            19947..20915
                     /gene="waaC"
                     /locus_tag="ACFOA3_000535"
     CDS             19947..20915
                     /gene="waaC"
                     /locus_tag="ACFOA3_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6897589.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21142..21891
                     /locus_tag="ACFOA3_000536"
     CDS             21142..21891
                     /locus_tag="ACFOA3_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6897590.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21902..22837
                     /locus_tag="ACFOA3_000537"
     CDS             21902..22837
                     /locus_tag="ACFOA3_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6897591.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            22900..23511
                     /locus_tag="ACFOA3_000538"
     CDS             22900..23511
                     /locus_tag="ACFOA3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6897592.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(23563..23880)
                     /locus_tag="ACFOA3_000539"
     CDS             complement(23563..23880)
                     /locus_tag="ACFOA3_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6897593.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24038..25309
                     /gene="purD"
                     /locus_tag="ACFOA3_000540"
     CDS             24038..25309
                     /gene="purD"
                     /locus_tag="ACFOA3_000540"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6897594.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGVLEKISFDGMQYRKDIADKAINR"
     gene            25343..25945
                     /locus_tag="ACFOA3_000541"
     CDS             25343..25945
                     /locus_tag="ACFOA3_000541"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6897595.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(26016..>26204)
                     /locus_tag="ACFOA3_000542"
     CDS             complement(26016..>26204)
                     /locus_tag="ACFOA3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6897596.1"
                     /translation="FADRQNHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRF
                     NFGTLSRQLKILQDWCGI"
BASE COUNT         6265 a         6028 c         7411 g         6500 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatacgtat ccccatccga aagtttattt atacacaagc
       61 aatccaaagt tatctgactg aaatattgtg agatttaccc gcaaatctca ttttttccac
      121 cacttataca caaattcgga tgcgcactct gccgatatgc agaaataaga cattcgtgcc
      181 gacacctgac catttccaca agttccaaac acactttcct ctcaaacctc cacagtattc
      241 cggttcttaa agacatcggc agccattcgg taaagaaaag cggaatgcca tctgcacaac
      301 aggcaaactg tccacaaaaa ctattcttca tgaaaaacaa tccacaggca tgcacaaaag
      361 ggaatccgcc ttaccgacac cctaaaaaaa agaaatcctg atgattttta aaataaaaaa
      421 aagttaccca cagaaaattt ctcctatcaa tataatcatc gttttattat ttaaatttta
      481 tattttattt ataagccttc agggaaacgg cacgggaaag cgtaaaggca tcttttcaaa
      541 aaattaaaaa gttaaggatt gttaaacaag tgtaaatcag gtagagttgt ggtcgataaa
      601 ttaaatctat aacggagctt cctgcaatga aaggcgatat tggcgtaatc ggtttggcgg
      661 ttatggggca aaacctgatt ttgaatatga acgattgcgg atttaaggtt gtcgcctaca
      721 accgaacaat cggtaaggtg gacgaatttt taaacggcgc ggctaaggga acggatattg
      781 tcggcgcgta ttccctgcaa gacttggtcg ataagctggc aaaaccgaga aaaatcatga
      841 tgatggttcg cgcaggttcg gtcgtcgacg actttatcga acaactgctg cccctgttgg
      901 acgaggaaga catcattatc gacggcggca atgccaatta ccccgacacg acacggcgga
      961 cacattacct tgccgaaaaa gggattttgt ttgtcggggc gggcgtatcc ggcggcgaag
     1021 aaggcgcgcg gcacggcccg tccattatgc cgggcgggga taagcgcgct tgggaagcgg
     1081 ttaagccgat ttttcaggcg attgccgcca aaacgccgct gggcgaaccg tgttgcgact
     1141 gggtcggcag ggacggcgcg ggacattttg tcaaaatggt gcataacggc atcgaatatg
     1201 gcgatatgca gttgatttgc gaagcgtacc agttcatgaa agacggtttg ggactgtcct
     1261 acgacgaaat gcaccgcgtg tttgccgagt ggaacaaaac cgagctggat tcttatctga
     1321 ttgaaatcac agcggcaatt ttgggctata aggacgaagg cggcgaaccg ctggtcgaga
     1381 aaatcctcga tacggcaggg caaaaaggca cgggcaaatg gacgggcatc aatgctttgg
     1441 atttgggcat tccgttgacg ctgatttcag aggcggtgtt cgcccgctgc gtttcatcgt
     1501 tcaaagaaca gcgcgtccgg atgggcggac tgtttgcgcg gcaggcattg ccagtcggcg
     1561 gcgataaaaa acaatgggtc ggcgatttgc gccaagcctt gctcgcctcc aaaattattt
     1621 cgtatgccca aggcttcatg ctgatccgcg aagcgggcga aagctacggc tgggatttgg
     1681 gctacggcaa cactgcgctg ctgtggcgcg aggggtgcat cattcgcagc gcatttttaa
     1741 gcaatatccg cgatgcctat gaaaacaatc ccgatttggt gttcttgggt gcggatccgt
     1801 atttcaaaaa tattttggaa aactgcctgc cggcgtggcg caaggtggtt gccaaggcgg
     1861 tcgaatgcgg cattcctatg ccctgcatgg cttcggcaat tacgttcttg gacggctaca
     1921 cgaccgaacg tctgcctgcc aacctcttgc aggcacagcg cgactacttc ggcgcgcaca
     1981 cttacgagcg caccgacaaa ccgcgcggcg agtttttcca taccaactgg acgggcaagg
     2041 gcggagatac cgcttcgaca acctacgata tttgattccg ttgccgaaat gccgtccgaa
     2101 ccgttcggac ggcatttgtt ttgcaggaaa tagaaagatg ttccaatggc tttatgatgt
     2161 attgtggctg cttgcgccga tatggatacg gcgttatttg gacaaacgct ccggaagtgc
     2221 cccggcatat cgggcgcatc gggacgagcg tttcggcaag ccgtatccga atcccgttac
     2281 cggagcggtt tggatacacg ccgtttcggt cggagaaacg cgtgccgccc agcccttgat
     2341 acgcgagttg cggcagcgtt ttcccgatgc accgctgctg atgacgcaga tgaccccgac
     2401 gggacgggaa accgcgcaag ttctgtttcc cgatgcgcaa tgccgctatc ttccgtatga
     2461 caaaaaaacg tgggtacggc agtttttgcg cgaacaccgc ccgatgttcg gcattttgat
     2521 ggaaaccgaa atctggccca atctgatgaa ggaatgccgg cgcacgggtg tgccgctgtt
     2581 tttggcgaat gcgcggctgt cggaaaaatc gctgaacggt tatctgaaag tccgcagcct
     2641 gatccgtcct gccgccgctt cgctgacggg ctgtctggca cagacggagg cggatgcggc
     2701 gcggttggcg aaattgggcg cggcatccgt gcaggtgtgc ggcaatacca aatacgacct
     2761 gatgccgtcg gaacagatga aaacgctggc ggggcagttt gaaaaacgca tcggagaccg
     2821 gcaggttgcc gtgtgcggta gcacgcgcgt ttatcggggc gaagacgagg cggaaaaact
     2881 gctggcggcg tggcaacaat atcgcggcga tgcgctgctg gtcgtcgtgc cgcgccatcc
     2941 cgagcatttt cagacggtat ttgaaacggc aaaacgcttc gggtttaagg ttcagcggcg
     3001 cagcgacggt ttgccggtcg aacctgatac gcaggtgtgg ataggcgaca gtatgggcga
     3061 gctgtatgcg tattacttgt gcgccgatgt cgcttttgtc ggcggcagtc tggtcgattc
     3121 gggctgtcag aacattatcg aaccgctttc ctgcggcgtt ccgacgatat tcggcttttc
     3181 aacctacaat ttttccgaag cctgccggca cgccttggca tcgggggcgg cggttcgggt
     3241 tgagtcggcg gacgcgtggc gggaagccgt tgaaaaaacc ttatcgtccg aggggggcgg
     3301 aatgcagatg caggcgcgcg tggacggctt tatcgcacaa catcgcggag cgggcgcgag
     3361 aatcgccgag gcggtgcggg aagcggtatg cggacatcgg gggcgatagt gatacaatcc
     3421 gtatccgagt tttccggttg gagcatcgta tgagtattta tgccgtcgcg cacatcgtcc
     3481 atctgtattg cgccatcgcc tttgtcggcg gcgtgttttt tgaagtgctg gttttgtctg
     3541 tcctgcatac gggacgggtg tcgtgcgagg cgcggcgcga agtggaaaag gcaatgtctt
     3601 accgcgccgt cagggtgatg ccgtttgtgg tcggactgct gttcgccagc ggcatcgtga
     3661 tggcggcaaa ccgctatctt tctatattgg gcgaaccgtt tgccacttcc ttcggtacga
     3721 tgctgacgct gaaaatcctg ttggcgttca gcgtgttggc gcacttcgcc atcgccgtcg
     3781 tcaaaatggc gcgttccaca ctgaccgtcg gctggtcgaa atacatacac accgtcgtct
     3841 ttacccatat gctgctgatt gtctttttgg caaaagcgat gttttatatc agctggtaac
     3901 acaattacat ttcaaatcag gagattgaac catgacagaa tatcccggca tcgggtcgcc
     3961 gttgttttac ggcgtttttt ttgcggcagt gctggtcatg attgccttgg atatgttttc
     4021 gctgaagaaa aacggcagcc ataaagtcgg cgtcaaagaa gccttggcat ggagcggctt
     4081 gtgggttgcc gtatcctgcc tgttcgcggg ctggctgtat tttgaactcg ccggcaatct
     4141 cggctacggc gcggcagtcg caaaagaaaa agtattggaa ttctttaccg gctacatttt
     4201 ggaaaaatcg ttggcagtcg acaatatttt cgtgttcctg atgatattcg gctacttcaa
     4261 agtcgcacca cagtttcagc accgcgtgct gctgtacggc gtattgggcg cattggtatt
     4321 gcgcactgtc atgattttcg tcggcgcggc actggttcag cagtttgagt ggattctgta
     4381 tctgtttggc gcgttcctgc tctatactgg tatacacatg atgaagcccg aaggcgatga
     4441 gaaagaggat ttggcaaaca gcaggctgct gaatgccgtc aagaaagtcg ttccggtcgg
     4501 cacggaattt cacggcgaga aattttttac cgtcgaaaac ggcaaaaaaa tcgccacgcc
     4561 gctgtttttg gtgctggtca tgattgaatt gagcgatgtc gtgtttgccg tggacagcat
     4621 ccccgccgtc tttgccgtta ccaccgatcc gtttatcgtg ctgacttcca atattttcgc
     4681 aattctgggc ttgcgggcga tgtatttcct gctggcggat gtggcggaac gctttatctt
     4741 cctgaaatac ggcttggcat tcgtgttggg ttttatcggt gtgaagatgc tgattatgca
     4801 ttgggtgcat atcccgattt ccgtttcgct gtcggtcgtg ttcggcgcat tgggtgcatc
     4861 ggtgttgact tcattggttt atacgaaaaa acagtccgat aaataaaatg ccgtctcaac
     4921 gccgacaaaa tggttgacag catcaggctt gatgtataga atttcgagct tatcgggtcg
     4981 ttagctcagc cggtagagca gcggactttt aatccgttgg tcgagcgttc gaatcgctca
     5041 cgacccacca gataaaacaa aaaagctgag ttttcaaaac tcggcttttt ttgtctgccg
     5101 acaatccttg ttcggaaagc gcggcatttc gtcaaattct ttggtgcggc ttccgtattt
     5161 ttcccgaaaa tcccttataa ttccgcctct gtaaccttca tcgggtgatg ctttatgcgt
     5221 tgtcccgagt attgaaatat tacaagtacc caccaggaaa gaacaaacgt ggacaaactg
     5281 aaaatctccg caaacggccc gctcaacggg gaaataacgg tctcgggcgc gaaaaacgcg
     5341 gcattgccgc tgatgtgcgc gggtttgctg acatcgggta cgttgcgcct gaaaaacgtc
     5401 cctatgctgg cagatgtgaa aaccacgcaa aagctgcttc aggggatggg cgcgcgcgtc
     5461 ctgaccgaca atatcagcga atttgaaatc aacggcggta cggtaaacaa tacctgcgct
     5521 ccgtatgagc tggtcagaac tatgcgcgcc tcgattttgg tgcttggccc gacgctggcg
     5581 cgtttcggcg aggcgcaagt cagcctgccg ggcggctgcg ccatcggttc gcgccccgtc
     5641 gatcagcatt tgaaaggctt ggaagcgatg ggggcggaaa tcgtcatcga acacggttac
     5701 gtcaaagcca aaggcaaact caaaggtacg cgcgtggcga tggatgtcgt taccgtcggc
     5761 ggtacggaaa acctgctgat ggcggcgacg ctggcggaag gtacgacggt tttggaaaat
     5821 tgcgccatcg agcctgaagt ggtcgatttg gcggaatgcc tggttaaaat gggcgcgaaa
     5881 atcagcggca tcggaacatc gaccatgatc gtggaaggtg tggacgagct gcacggctgc
     5941 gaacacagcg tcgtccccga ccggattgaa gcgggtacgt tcctgtgtgc ggtggcgata
     6001 accggcggca gggtggtttt gcggaatgcc gccccgaaaa cgatggaagt ggtgttggac
     6061 aaactggtcg aggcaggtgc ggtgattgag gcgggcgacg attggatcgc catcgatatg
     6121 cgtcaacgtc cgaaggcggt ggacatccgc acggtcgtcc accccggctt ccccacggat
     6181 atgcaggcgc agtttatggc attgaatgcc gtggcggagg gaagctgccg cgtggtggaa
     6241 acgatttttg aaaaccgctt tatgcacgtc cccgagttga accggatggg ggcgaacatt
     6301 acaaccgagg gcaatacggc attcgtgcag ggtgtggaac agctttccgg cgcggtcgtc
     6361 aaggcgacgg atttgcgcgc ttccgccagc ctcgttatcg ccggtttggc ggcgcgcggc
     6421 gaaaccgtgg tcgaacagat ttaccacttg gatcgcggtt atgaaaatat tgaaaaaaaa
     6481 ctcggcagtg tcggcgcgag aatcgagcgc gtatccggct gatcgggttg cgatgccgtc
     6541 tgaaggcttc agacggcatt aattgtttct gtatcgcaaa cactgattga aaaggactga
     6601 ttatggcatt tttaaaactg accgaacaaa acgtgcaggg caaaaccgtc ctcatccgcg
     6661 ccgatatgaa cgtgccgttc aaagacggca aaatcagcga cgacacccgt atccgcgcct
     6721 cgcttgcgtc cgtcaaatac tgcttggaca acggtgcgtc cgttatcgtg atgacccacc
     6781 tcggtcgccc gaccgaaggc gagttccatc ccgaagacga cgttgcgccc gttgccgcgc
     6841 atttgggcgg tctgttgggc aaagacgtga aagtattgaa cgactggcgc gaaaacaaac
     6901 cggctttgaa cgcgggcgat gtcgtgatgc tgcaaaacgt gcgtatcaac aagggcgaga
     6961 agaaaaacga tttggaactg ggtaaagcct acgcttcttt gtgcgacgtg ttcgtcaatg
     7021 acgcgttcgg caccgcacac cgcgcccaag cctcgaccga agccgttgcc caagttgcgc
     7081 ctgttgcctg cgccggcgta ttgatggcgg gcgaactcga cgctttgggc aaagccctga
     7141 aacagcccgc gcgcccgatg gtggcgattg tcgccggcag caaagtgtcc accaagctga
     7201 ccatcctcga atcgctggcg gacaaagtcg accaactcat cgtcggcggc ggtatcgcca
     7261 acaccttcct gttggcggaa ggcaaagcca tcggcaaatc tttggcggaa cacgatttgg
     7321 tggaagaatc caaaaaaatt atggcgaaaa tggcggccaa aggcggttct gtgccgctgc
     7381 cgaccgatgt cgtcgttgcc aaagcctttg ccgccgatgc cgaagcagtg gtaaaagaca
     7441 ttgccgacgt tgccgaagac gagatgattt tggacatcgg cccgaaatcc gccgccgcgc
     7501 ttgccgattt gcttaaagcc gccggtacgg tcgtttggaa cgggccggtc ggcgtgttcg
     7561 agtttgacca attcgcaggt ggcacgaaag cccttgccga agccattgcc caaagcaaag
     7621 cgttctcgat tgctggcggc ggcgacacgc tggcggcgat tgccaaattc ggcgttaccg
     7681 aacaaatcgg ctacatctcc accggcggcg gcgcgttcct tgagttcttg gaaggcaaag
     7741 agctgcctgc cgtagctgct ttggaaaaac gcggcgcgta agcggtttga tataaaaaaa
     7801 atgccgtctg aaaccatttc gtttcagacg gcattttgta tggtggttat tgtgctttgg
     7861 cattccattc tgccggtgtg gcggctactg aaatggaaag tgcgtgcagt tcgttgcttt
     7921 ccagttgggc tttaagcccg tctttaatta ggcggtggcg tgcaaggcgt gctttgcctt
     7981 caaattctga tgaaacgatg acggcgaaaa aatggtgtcc gtcgccttct acttcgatat
     8041 gttcgcattt tgccacgcct gcaatcaggg ccttgacttg ttcgggtgtc agcatagtgg
     8101 ggagtcctta ttgggaaatg cgccattata ctggcaaatt gaaatgccgt ctgaaaacgg
     8161 gttcggacgg catttttgac ggtgttttat tttttggctt tgaagccgag caggtgctgg
     8221 gtggtggcaa gccacgcgcc gaatacgccc aacgcgatga cgaagccgaa caccagtgcg
     8281 atttctcccg ggtagaagaa acgccagccg atattaagtc cgtagggttt gaagatggca
     8341 tcgaccaagg ggcgcacggc agagagcagc caaccgcaaa gccccaagct gacggcggcg
     8401 gaaaggatgc tctgccacat ggcttgataa aggaatgggc ggcggataaa cgacgcgggc
     8461 gcgcccaaga gtttggtgat ttcgatttct tctttgcggc tgaggatttg cagtcggatg
     8521 gtgttgtgcg cgacaaggac gaacgccatc cccagcgtca gggaaagaaa ccataagatt
     8581 ttacggatga attcgttgat ttggtacagc gtttgcaccc attcggtatc catagacgcg
     8641 gattcgacca taggcagttt ggtaatgtct cggtagattg cctgcatttg ggcgggcgtg
     8701 gttgccgggt cgggggtaac gataaagaca tccggcaggg ggttgccgtc aagcatggaa
     8761 atcagatttt ggtcgagatt ggactgtaat tcttccagac cgtcttcttt gccgatgaag
     8821 cggatgttgt cgagccgttt gtcgcgcgcc agcaggctgc ggacggtatc gctgtcgctt
     8881 tgtgcggcgg cggtttccat atagacggtg atttgcggcg actcattgag tttgcccaac
     8941 acgctttgcc cgctttggat gcccaagtac ataaacagcg gcagggtcat ggcgacggcg
     9001 agcatcagaa gggtcagtat cgtgccgaca ggttggcgca tgagttgttt gagtgcggtg
     9061 cgtgcggatt cgacgtgcag cgagacgtag tgaatgatgc tcatgcgaga cgtcctttcg
     9121 agaggcgcag gatgcggtgt ccgtagtccg ccatcagggt ttcgtcatgt gcggcgacga
     9181 tgacggttgt tcccgcttcg tggaaggttt tgaacaattc cataatatcg agcgcgtagg
     9241 cgcggtcgag gttggcggag ggttcgtcgg caatcagcag gccgggctgg tgaacgacgg
     9301 cgcgggcgat gcacaggcgt tgttgttcac cgccggagag ggttacggga tcgtccaatt
     9361 ctcgtccttt caggccgact ttttcgatgg cgatgcgggc gcgttcttcg gctttgcgcg
     9421 gcggatagcc gataatccga agcggcagga tgacgttttg caggacgttg cggtcgtaga
     9481 ggattttgtg gtcttggaac acgatgccga tgtgttggcg cataaagccg atttggttgt
     9541 cggacaatgt gccgaggtcc tgcccgttaa acaggatttt gcccctgctc ggcttggtga
     9601 tgcccgaaat cagcttgagg acggtggatt tgcccgaacc ggagtgtcct gcgataaata
     9661 tcatttcgcc tttgttgatt tggaagctga cgtttttcag ggcttcaaaa ccgccgggat
     9721 aggttttgga aacttgttcg aaacggatca taggaggtcc tgtaaggaag ggcggcatat
     9781 cgtttcgggc ggttgcgtcc gcagggatat gccgtctgaa caataggtaa gacagtgccg
     9841 attataaaga ttccgcctgt ttttagatag tggataagtt tggcggatac cgaaggtcgg
     9901 gcgggaaaaa tgccgtctga agcggattca gacggcatcc ggcgtttaac ggcattttga
     9961 atgggcgagt ttgacggctt cggtcaggct tttttcgttt gcctcccctg taatggtctg
    10021 cctgtatccg catttcggtg cttcaacgac ggtaaagggc agtacgccga cagtgtttcc
    10081 gtaggttttc ataaagtttc ggctgtttgc cccggtgtaa cgccaaatcg ggtaggaaac
    10141 aggagtttgt ttgaggaagt tgccgatatt gtcggatgtg tcgagcgcga tgccgaccat
    10201 atcgacgctg ccttttttct gcgctttgta ccatttggac atggcgggca tctcttttcg
    10261 gcacgggccg caccaggttg cccaaaggtt gacgatgcgt acgggggctt tgagtgattg
    10321 caggctttgc ggggtgttgt ctttccatcc ggccagttcg tctgccgagg cggtatgtgc
    10381 ggcaaatgtg gcggcaaggg tgattgcggc aagattcaag tatttcatcg ttttgtcctt
    10441 tgtatgtcga ttcgggtatt gtaaaccgtt tggtacggtt gtaggcggga tattgagtaa
    10501 aaccctgtgt aagccgataa taaggggatt gtcgagaagt aaggaaatgt catgtctgaa
    10561 atcaaaattt tccacaatcc gcgttgcagc aaatcgcgtg ccgccttgtc cctattggaa
    10621 gaacgcggca ttgctgccga agtggtcaaa tatttggata cgccgcccga cttgtccgaa
    10681 ttgaaggata ttttcaacaa attgggtttg gcatcggcgc gcgggatgat gcgcgtgaaa
    10741 gatgatttgt ataaggaatt gggtttggac aaccccaatt tggacaatga cgcgctgctg
    10801 cgtgccatcg ccgatcatcc cgccctgttg gagcgtccga ttgttttggc gaacggtaag
    10861 gcggctgtcg gcaggcctct ggaaaatatc gaagcgatat tgtgaacaaa cggctgtttt
    10921 gcagccgcaa cggcttgcgc tattacctgt tgggcggttt ctgtttgagt gtttttccgt
    10981 ttttgctggt gtttgcctca tccgtttggg cggtttatcg gacgggcggg caggttttgc
    11041 cgccgtatgt ccgggcggat gccgcgctgg tgttaggtgc cgccgcgtgg gacaaacgcc
    11101 cttctccggt ttttcgggaa cgcatcaacc acgccatcgc gctttatcag agccgcaggg
    11161 taggcaaaat cgtgtttacg ggagggcgaa tcaaaaaagg ctatatgacc gaagccgagg
    11221 tcgggcggcg gtacgcgctc aaacaggggg ttccggcgcg caacatcctg tttgaaaaca
    11281 cttcgcgcaa cacttatgaa aacctgaaca acatccgtcc ggttttgcgt gccaacggta
    11341 ttgccagcgt ggtgattgtc agcgatccct accatttggc gcgggcggcg gaaatggcgg
    11401 aggatttggg tgtccgcgtt tatatgtctg ccaccccgac cacgcggttt gatgcgggaa
    11461 ataagaaaaa aatctttatg ctgcaagagg gatatgcgtt atccctctac cgtttggaaa
    11521 aatgggggcg ggattttttc aattggctgt ttcattgaac ctttttttcg gacgcaatcc
    11581 cgaaggaacc cggtcggcgg cgcgtgatac actgttccgc gtgccgaagc attgaaaaca
    11641 aggaaaatgc cgtctgaaac ttatccgcag gtagggttcg gacggcattt ttaatgccca
    11701 agcacggtaa aaccagtaaa ataaacgctt tcaaagattt cggagccatt atgaccgtca
    11761 aaacccgttt cgcccccagc cccaccggct acctgcatat cggcggcgtt cgcaccgcct
    11821 tgttttcatg ggcgtttgcc cgccatcata aaggcgaatt tttattgcgt atcgaagaca
    11881 ccgacttggc gcgttccacc gccgaatctg tcaacatcat cctcgacggt atgaaatggg
    11941 tcggactgga ttacgacaac gccgacaacg tggtgtacca aacccgccgc ttcgaacgct
    12001 ataaagaagt catcgccgaa ctcttggcaa aaggcgctgc ctactactgc tattgcagca
    12061 aagaagaatt ggaagcgatg cgcgagaaag ccgaaaaaga aggcacggcg acttacgacc
    12121 gccgctggcg gccggaagca ggcaaaaccc tgcccgaaat tcctgccggc gtgcaacccg
    12181 tcgtccgctt caaaacgcct ttggacggcg taaccaagtg ggcggacctg gtcaaaggcg
    12241 aaatttccat tcccaacgaa gccatcgacg acctgattat cgcccgcgcc gacggcacgc
    12301 cgacctacaa cttctgcgtc gtggtggacg actacgacat gggcgttacc cacgttatcc
    12361 gcggcgacga ccatgtgaac aacacgccga aacaaatcaa catcttgaaa gccatcggcg
    12421 cgaacctgcc cgaatacgga cacctgccga tgattctcaa cgaacaaggc aaaaaaatct
    12481 ccaaacgcag cggcgatacc gtcgccatca ccgatttcgg cgcgatgggc atcctgcccg
    12541 aagcgatgct caactatttg gcgcgtttgg gctgggcgca cggcgacgac gaatttttca
    12601 cgatggaaca attcatcgaa tggtttgatt tgaaagacgt ttccccatct ccaagccgca
    12661 tggatttgaa aaaactctat tggataaacg gcgaacacat caaaatcacg cccaacggca
    12721 aactcgccga actcgtcaag ccgcgtctcg ctctgcgcga tatccatgaa actgaaaaac
    12781 ctgcattgga agacgtgttg gcactggtca aagaccgcgc ccaagatttg aacgccttgg
    12841 cagacgaatg cctgtatttc tacaaaaaac aagtccctgc cgaagccgat gttgccaaac
    12901 actgggatga cgaagcggcg gcacgcatgc tgcgctttgc cgaatgcctc gaagggctgg
    12961 aagactggaa tgccgaagcc atccacgacc tcttcaaacc tttctgcgac gaagaaggca
    13021 tcaaaatggg taaactcggt atgcccctgc gccttgccgt ctgcggtacg gcgaaaaccc
    13081 caagcgtcga tgctgtgttg gcattaatcg gcaaagaaga agtgttgaaa cgcatccgcg
    13141 cttaagttat ccacaagcgt gggcgatgcc atctgatgtc ggaatgcggc ttcagacggt
    13201 atttaaacgg aagttgaaca tgaaaaaaat attttatttt ctgatgtttg ttttttctac
    13261 aagcgtatgg gcaggggatg cggaagacaa tctgctcagc atccaatccg gctatcgtgc
    13321 cttactgcaa aaacaaaaca atctggacgg aaaaatcatc gggatgcagt cggatttgga
    13381 agatgcgcgc cggcgtttgc agacggctca ggcagacatc gcccgtttgg aagcggaaat
    13441 tcctgcagca atggcgcaaa aagcccggca ggctgaagag ctgaggcaaa tcggagtgcg
    13501 tttggaccat gcttggaatg cggtttacgg cgcaggggga acgaaggcgt cggggaattg
    13561 accgatgaat tccctctttg tggacaatac tgttttcatt acacggctga aagccgggca
    13621 tatcggcagg ttggttcagg cgttgtttga ggagtggtac ggatttgaac cgtggtcttc
    13681 tgtggataag attcgtgcct attacggcag atgtttgaaa gatgacgaac tgccgctggc
    13741 ttttgcggct gtggatgatt tcggaaccct gttgggttcg gctgcggtca agcggcatga
    13801 tatggaaagt tttccacagt atgaatattg gttgggtgat gtctttgttt tacctgaata
    13861 tcgcggaaaa ggcattggca ggaggctggt cgcccactgc ataggcgcag cgcgttcgct
    13921 ggggataaag ttcttgtatc tttatacgcc tgatgtgcaa atattttatg aatcattcgg
    13981 ctgggcggtt gtcgggcgac atttccataa cggtgaatgg gttacggtta tgcgtttgga
    14041 tatggataag gtttaagccg aatggacaaa tgccgtctga gctgttcgga cggcattttt
    14101 tatgtttgga aacttcctgt cagataatat ttatccacag atttttccat atggtttgat
    14161 gctgttccga caacggaaat taagatacgg atttgcacaa atctttcaga cggtacagtt
    14221 ctgacaatgc ttcgcgcggg gtcaggctgt cgggatcgag tttctccagg gctgctgcca
    14281 atataccttc aaaatgtttt tcctctgctt tgtccacaaa gctgtccaca ttcggttcat
    14341 ctcctttttc agacggcatg gtactgaaaa tatccagttg gggacggttt gcggcggctt
    14401 ggtcttccag ttcgttcaaa tgcttttggg cggatttcaa tgcgcgtaca ggcaggccgg
    14461 cgagtttggc gacggcgatg ccgtagcttt ttccggcggg ccccggttgg atttggtgca
    14521 ggaacacgat gtcctgtccc tgttcgagcg cggaaaggtg catattgacg gcggcggcgt
    14581 gggtttcggg caggtaggtc agctcgaaat agtgggtggc aaacaggctg aaggatttgt
    14641 ttttttgcag caggtgttcg gcaacggcgt gcgcgagggc aagaccgtcg aaagtggaag
    14701 tgccacgtcc gacttcgtcc attaaaacaa ggctttgttc ggtggcgtga tgcaggatgt
    14761 aggcggtttc gctcatttcg accatgaaag tggagcggtt ggaggcgagg tcgtccgatg
    14821 cgccgatgcg ggtgaagatt tgatcgatgg gcccgattgt ggcggcatcg gcaggcacaa
    14881 aacagccggt gtgtgccaat aaaacaatca gcgcgacttg gcgcatgtag gtggatttgc
    14941 cgcccatatt ggggccggtg agcagcatga ggcggtgttt gtggtcaagg tcggtgtggt
    15001 tggcggtgaa gtggcgtacc tgctgttcga caacgggatg gcggccgttt tcgatgtgga
    15061 taaccggata gtcggcaaac tcggggcgga cgaagttccg ctcttttgcc aaggctgaaa
    15121 atgtggacaa cacgtccagc gcggcggcgg ctttggcggc tttttgaagc tgcggcaatg
    15181 ccgtctgaag gtttttcaat acgccgtcaa agagttgttt ttctaaggcg agggcttgct
    15241 cttgagcagt cagcactttg tcttcaaagg ctttcaactc cggcgtgatg aagcgttcgg
    15301 cgtttttgag ggtttgccgg cgttggtagt cggcaggtgc ttgttcggct tgggttttgg
    15361 acaattcaat gtaaaagccg tgaacgcggt tgaactcgac tttgagtgtg gacaaaccgg
    15421 tacgttcgcg ttccttggct tccaaatcca gcaaaaattc gtcgccatgg ttttgaatgc
    15481 ggcgcaattc gtccagttcg ggatgaaaac cgtggttgat gacattgccg tctttcagcc
    15541 agacggaagg ttcgggcaaa atggcttggc ggagctgttc ggctgtggat aggttttccg
    15601 ggaaaacggc tttgagggtt cctaagagac tgctgcactc ggcggacaat tcgatttcgg
    15661 acagggcaaa caggctgtcg cgcagggcgg cgaggtcgcg cgggcgggcg ttacccacgg
    15721 caatacgggc ggcgatgcgt tcgatgtcgg caatgctttt cagacggcac tggaggggtt
    15781 tgtattggct ttccagcgcg gcaacggctt cttggcgcgc tcggatgtgg gcgcggttgc
    15841 gtaaagggtg gtgcagccag agagccaaga ggcggctgcc catatgggta gcgcaaaggt
    15901 cgagcgtgga catcagggtc ggcgattttt tgccggagag ggtttgcgtg atttcgagat
    15961 tgcggcgcgt ggcggcatcc ataccgatat attggctgtc ggtttcgagc gacaggccgt
    16021 ccaaatgttg cggcatcagg ttttgcgtca gacggatata gttcaacagt gcacctgccg
    16081 cgccaatcgc aacggcgtgt tctttgccgt ccaaaccgaa gccgcgcaaa tcctggcagc
    16141 cgaaatattc cgtcagcagt ttttcccccg cgtcggcggc aaactgccac gcgttcaggc
    16201 gcgtaacacc cgatgccgtc tgaagttgcg gtgcgttttt actgtccggc aacagaattt
    16261 ccgccgcctg caggcgcgcc agttcgtcgt ccaatttatc cacagttgtc agcttggttt
    16321 tgaattcgcc gctttgcagc gatgcccacg ccaaaccgat gtattttttg tcgggggaca
    16381 cggcaacgat gcggttggtt tccttgtctt ccagcaatgc ggaatcggtc agcgtgccgg
    16441 gcgttacgat gcgcacgact ttgcgctcca ccggtccttt gcccgcgccg acttcgccga
    16501 cctgttcgca aatcgccacg cttttgccca acttgaccag gcgcgccaga tattgttcgg
    16561 cggcgtgaaa cggcacgcct gccattttga ccggctcgcc atccacctgt ccgcgcgtgg
    16621 tcagggtaat atccaaaagt tttgccgctt ctaccgcatc gtcgaaaaac atctcgtaaa
    16681 aatcacccat gcggtaaaac accagtttgt cggtatgttg cgctttgatg ccgaggtatt
    16741 gctgcatcat tggggaaacg gcggatttgg acatggggct gccttatcat taggaaaacg
    16801 gtattgtagc aaaaggtttt gtcttcgacg gaatgagact gaccgtaagc ctgtcaataa
    16861 ggttgtggag acaagtagga aaacagcaaa gttatccacg gagatattat taagtcaaaa
    16921 aacatccacg tttatctgtc ctatacggca agccttaaac tcaaacttat ttgacgaaaa
    16981 ggtaatgaaa atattggata tttgcaactt atacacaagc agatccgcaa tcatcaaaat
    17041 catcattaaa attttaaatt caaatattga tggtaaaagt tttggaaatc tgtgcaaaca
    17101 gtaaaattca tctatatcaa ataagcatca agtagttaga tgtaatagtg gaatgtaact
    17161 ttatttcaga atacaataca gatattgaaa tttcgtttca cagcttggag tatatacatg
    17221 agcattaaag tagcgattaa cggtttcggc cgcatcggcc gcctcgcatt gcgtcaaatt
    17281 gaaaaagccc acggcatcga agtcgtcgct gtcaacgacc tgacccctgc cgaaatgctg
    17341 ctgcaccttt tcaaatacga cagcacgcaa ggccgtttcc aaggtactgc cgaattaaaa
    17401 gacgatgcca tcgtggtaaa cggcaaagaa atcaaagttt tcgccaatcc gaatcccgaa
    17461 gaattgcctt ggggcgagtt gggtgtggat gtcgtcctcg aatgtaccgg cttctttacc
    17521 aataaaacca aagccgaagc acacatcagg gccggcgcgc gcaaagtcgt gatttccgca
    17581 cccggcggca atgatgtgaa aaccgtcgta tatggcgtaa accaagacat tttggacggc
    17641 agcgaaaccg ttatctccgc tgcttcctgc accaccaact gccttgcccc gatggcggca
    17701 gtcctgcaaa aggaattcgg cgtagtcgaa ggcctgatga ccaccatcca cgcctacacc
    17761 ggcgaccaaa acacccttga cgcgccgcac cgcaaaggcg atttgcgccg cgcccgcgcc
    17821 gccgcgctca acatcgtgcc caacagcacc ggtgccgcca aagccatcgg tctggttatc
    17881 cccgaattga acggcaaact cgacggctcc gcccaacgcg tccctgttgc caccggctcg
    17941 ctgaccgaat tggtttccgt cctcgaacgc cctgtaacca aagaagaaat caacgccgcg
    18001 atgaaagcag ccgccagcga atcttacggc tacaacgaag atcaaatcgt ttcttccgac
    18061 gttgtcggca tcgaatacgg ctcgctcttc gatgcgaccc aaacccgcgt gatgaccgtg
    18121 ggcggcaaac aattggtgaa aaccgttgcc tggtacgaca acgaaatgtc ctatacctgc
    18181 caactcgtcc gcactttgga atactttgca ggcaaaatct aaggcttgaa caaacctgtg
    18241 gataagtaaa tgccgtctga acacattgcg ttcagacggc attgtttatc tgtatagtgg
    18301 attaaattta aaccagtacg gcgttccctc gccttgccgt actatttgta ctgtctgcgg
    18361 cttcgttgcc ttgtcctgat ttaaatttaa tccgctataa cttgatattg cggcttatgg
    18421 cgaacaaaca acagccgttt gttcaactgc tatgcggcaa aaccggattt ttgcccgatt
    18481 taaagcgggg ttggggaaag atcaataacg gttgcacggt tttcctcgat ttgaaatctg
    18541 acttcgtaat ccgcaatatt catcacataa atccgttcgg gaatatcctg ataggctggg
    18601 cgcggatctt gggcaatgct ttggctgata aggtttttgg tgtttgcaga taaattttcc
    18661 gcgccgatgt tttcctgcca aacgacttcc aactctacgg gtttgccgct gacgaaacca
    18721 gatgcggcat cgggtttgga ttcgacaaag gggatataag gtttgatgtc cacaatcggt
    18781 gtgccgtcca gcaggtctgc accgctgcaa tagaggcgga cgggtttgcc ggtttcgatg
    18841 cgttcgagtt tcaggagcga gagtccgaga tggttggggc ggtgggggct gcgcgtggcg
    18901 aacacgccca ttttttgttt gccgccgagc cgtggtgggc gcaccatttg cgcccagcct
    18961 tcatccaata cgccgtgaaa aataaaactt atccacacat agtcgaagtg ttccagtccg
    19021 cgtacgctgt ctgcggtaaa ttcgggattc agctcgatgc atacttctgc ggcagagacc
    19081 aaaccgggct ggcgtgcgat gccgaatttc tgtttgtagg gcgatcgggc ggtggcgata
    19141 gggacgatgg tgtaagtcat gaggattgtg gataagtttt tcggaaaaac cttatcttat
    19201 cacagcctgt tttaccgctt ttgggcagta atttgttata atacagggca ttttaattgc
    19261 gttgaaatga tgagatgatt gtttccattg atgttgatgc acaaaaaact tttacgcctt
    19321 tgtgtcctaa agagctgcct gtaaacgagg ggcatttgat tgtcgaagaa ttgaatgccc
    19381 aagccgcttt ggcggatttg cgcgtgatga cgaaagatgc gcatcatgcg gcagcaaaat
    19441 ggcttgtgga taaccctgtt gatatgttga agccgacagg tttgcccgat gcggatttga
    19501 cttgggtggc tcatgcgatg gttggtacgc gcggctatga attgttggac gggctgcctt
    19561 gtgccaaaga atacgattat tgcgtttgga aaggtgttga tcctgaattg catccttacg
    19621 gcgcgtgttt tcacgatatt gaggaaaaac taagcacagg gctgattgaa tggctgcgtt
    19681 gtcaaaatac ggatacggtt attatcggtg ggttagctac ggattattgt gttaaaacaa
    19741 cggttttgca gttactcaaa ggcggtcgtt ggcaggttgt cgtcaatgaa gcggcttgtc
    19801 ggggtattgc gccggaaacc atcgaaacag catggcagga aatgcattct gccggggcaa
    19861 tcatcttaaa aaacgctgat gaaataaata aatatattaa taatcaataa gttaaaattg
    19921 tttcacgtga aacccccttc ctaaatatga aaattttgct tgtccgcttg tctagtatgg
    19981 gcgatttaat tcacactttg cccgcaatcg aagatttggc gcgacaatgt cctgatgtag
    20041 aactgcattg gctatgtgag gctggatttg cagatattgc gcgcctgcat ccgtttgtaa
    20101 aaaaaatcca tgtgatgaaa tggcggcaat ggcgcaaaca tctctttcgg gctgaaactt
    20161 ggcgggaaat gggtcatctg aaacagactt tgcggcagga agtatttgat ttcgtattgg
    20221 acagtcaagg tctgattaaa agcgcgtgtt tcgctaaaat ggcaaaatcc ccgatttgtg
    20281 gtttggataa aaacagtgcg cgcgagggat gggctgcttt ggcgtatgta aaaacatacg
    20341 ctgtaccgaa gggaaaaaat gccgtttggc gcaaccgtga actgtttgcc caagtatttg
    20401 ggtatgtaat gccggaaacg caggtatttg gcttgactgt tcctgaagca ggccgtctga
    20461 aaaatttagc gcagccgtat tatgcggctt tgcatgcgac cagtcgggac agtaagttat
    20521 ggcctgtgga aaactggcgg tcgttgctgc aaaagttgaa tgaagaacag caatgcaata
    20581 tttacctgcc ttggggaaat gaagatgaaa aaacgcgtgc caaacaaatt gcagacggac
    20641 tgccgtttgc tattgtgtgc gacaaaataa atttattgca ggcagcgtat ctgctgaaat
    20701 acgcggtcgg aattgtcggc gtggataccg gtttgctgca tttggcaaat gccttggaaa
    20761 aacctgtggt cggcatttat accgataccg atccgattaa aacaggcgtt caggtttcgc
    20821 caattgcaaa aaatttgggc aatatcgggc agattccgac cgcagatttg gtttatcaag
    20881 cgctgatgga ttgtgttgca gcagataaag gctaaagggt cgtctaaaac tgatatggcg
    20941 gcatgatatt ttccaattta ttagatacag gaatgtatat ccgctagtct gagtaaatgc
    21001 tttaaaaatg gtataatcgg caagcattat ccgaaagatt tttgaatgaa ttcgttttta
    21061 aggaaaacgg atttggtttt atagggaaga aggtttggtt tcgctggatg gtgaaagcag
    21121 cgttcttttt aggagggttc aatgaaagca ctggtcgcag taaagcgcgt agtggactac
    21181 aacgtcaaag ttcgtgtaaa agccgatggt tcggatgtgg atatcggcaa tgtcaaaatg
    21241 tcgatgaatc cgtttgacga aatcgctgtg gaagaagccg tccgtttgaa agaagccgga
    21301 aaagtaagcg agatcgtagc ggtttctttg ggcgaaaaga aatgcgaaga aacgttgcgt
    21361 acagctttgg cgatgggtgc cgaccgtgca attcatgttg aaaccgatgc gaaattggag
    21421 ccgctggcag ttgccaagtt gctgaaagcc gttgcggaca aagaaaatcc gcaaattttc
    21481 tttttgggta aacaagcgat tgatgacgat gctaatcaag tggcgcaaat gctggcagct
    21541 ttgttgaatg cggcgcaagg tacgtttgct tcaaaagtac aaattgaagg tgatgaagta
    21601 cagattgtgc gcgaaattga tggcggcgaa gaaaccatag cattgaaatt gcccgctgtt
    21661 atcagtgcag atttgcgttt gaacgagccg cgcttcgtca aactccccaa tattatggcg
    21721 gcgaagaaaa aacctttgga aaaactggct cctgccgatt tggttgctga catttcacct
    21781 cgtctgaaaa cggtgaaatt cgccgagcct aaagcgcgtc aggcaggcgt aaaagtagca
    21841 agcgttgccg aattagttga aaaattgaaa aacgaagcca aagtgatttg aggagactga
    21901 aatgagcgta ttgattattg ccgaacacga caacaaacag ttgaatcctg ccactttgca
    21961 tgctgttacc gctgccgcaa aactaggcaa agtcgattta ttggttgccg gaaacggcgc
    22021 atcgtccgtc gtggagttcg cgaagcaagt agcgggagtg gaaaaagttt tggttgcaga
    22081 tgctgcccat tatgctgaag gtttggctga agagttggct ccgctggttg tcaaattggc
    22141 ggcggattac cgctatgttg cagcaacggc aaccacattc ggtaaaaacc ttttgccccg
    22201 cgtagccgcc ttattagacg taccgcaaat ttctgatttg accgagattg tagataacac
    22261 gacttttgta cgtccgattt atgcaggtaa tgcatttgaa accgtgcaag ccaatgcgga
    22321 aaaattggtg ctgaccttcc gtgcgacggc ttttgacgca gcggcggtgc aaggtggtaa
    22381 tgctgaggta attaatattg aaacaacccc tgcccaaaac ctgagtcgtt ttgttaaccg
    22441 tcagctttcc cattccgatc gtcccgaact gactcaggca aaagtgattg tttctggtgg
    22501 tcgtgcgttg ggtagtgcgg aaaaatttaa tgaagtgctg acaccgttgg cggatgtttt
    22561 aggtgcggca atcggtgcat cccgtgcagc agttgatgcc gaatatgcgc caaacgatgc
    22621 tcaagtcgga caaaccggta aagtagttgc gccgcaactc tattttgcaa tcggtatttc
    22681 aggtgcgatt caacacgttg ccggtatgca agacagtaag gtgattgttg caatcaataa
    22741 agatgctgat gcgccgattt tcaatgtagc cgattacgga ttggttggcg atttatttga
    22801 aatcgttcca caattaacag aagcattgaa aaattaatgt ttcacgtgaa actttcagac
    22861 gactttgtta ttaatgaggt tgtctgaaaa cttttcaaca tgattacgac ttataaaacc
    22921 attacttccc cgacgcaggc tgggtttaaa gataaaggca gccgttttat cgcatttgcc
    22981 tatccgattc ggacattggc tgatgtgaaa aaatacctcg atccgttaaa ggaagagcac
    23041 cataaagcac gacactggtg ttatgcctat cgtttgggcg tggatggtgt gcaatttcgt
    23101 gccaacgatg atggagagcc atcaggaagt gctggacgac caattttggg acagattgat
    23161 tcggtgggca tcaccgatgt tttggtcgtg gtcgtccgct atttcggtgg tactttattg
    23221 ggcgtttcag gtctgataca tgcgtacaaa gaggcgacgg ctcaggcgtt ggcgattgcg
    23281 gaagtgattg aaaagaatat tgaaaaaacc gtttggctga aatgtgaata tccatttttg
    23341 aatgaagcga tacgcatcgc caaacaatat caggcggata tattggagca ggatttgcag
    23401 ttggattgcc gattgacggt aagtttgtcg ttggcaaatt atgaagtctg tgttgcggct
    23461 tggaagaata cacggcagat tgaagtaaat acggaaaagc cttttgaatg aaaggtcgtc
    23521 tgaaatgctt tgtgatggta tttcagacga cctcttcgct gactaatctt cttgtgtata
    23581 tttgtctaag gcatcgacgc tggagcgcaa atataaccaa gccagttgat ggaattcgcc
    23641 cagcgcgttc catagcgcat cttcactcat aatgctgtat tcgccttccg tacgcaaatc
    23701 cagttctacc ccgtcttcaa tagcctgatg acacgcctga tactcgtggg tatagaaatt
    23761 gtccatgtcg cgaattaaag tgacgacata tttccaacgg cggatgtctt cttccagttg
    23821 cggtagtaaa tgacaccatt cgcggtattt gctttggatt tcatcaatac gtttttgcat
    23881 ggttgcttgt ccttttttaa tttatggata agttgaatag gttgcagagt ggaatgaagc
    23941 ggtcttttgt ttgattctac taaaatttct tcagatggga taaaagtccc tttgtatcga
    24001 gttttcgtta aaagtacata tttgtcaagg agatggtatg aaactgctgg ttatcggtaa
    24061 tggcggtcgc gaacacgcgc tggcttggaa attggcgcag tcgcctaaag tggaaacagt
    24121 atttgttgcg cctggtaatg ccggtacagc gattgaaccc aagttgcaaa acatcgactt
    24181 gactgcgcat caggatttga ttgaattcag tcgtaaagaa aatattgttt ttaccgtcgt
    24241 cggccctgaa gcgcctttgg cggcaggtgt tgtggatgat ttccgtgccg caggactaaa
    24301 aatattcggt ccgacacaat atgcggcgca gttggaaagt tccaaagatt tcgccaaagc
    24361 atttatggcg aaatacaaca ttccgaccgc gcaatatcaa acctttgaaa acgccgatgc
    24421 cgcacatgat tacgtcaatc agaaaggtgc gcctatcgtt atcaaagccg atggtttggc
    24481 ggcaggtaaa ggcgtgattg tggcgatgac tttggatgaa gcgcatgctg cgattgatga
    24541 tatgctgctg gacaacaaaa tgggcaatgc cggcgcgcgt gttgtgattg aagatttcct
    24601 gcaaggcgaa gaagcgagtt ttatcgtcat ggttgatggc aataatgtac tgcctatggc
    24661 aaccagccaa gaccacaagc gtcttttaga cggcgacaaa ggtctgaata cgggcggtat
    24721 gggtgcgtac agccctgcgc ctgtggtaac gcctgttgtg tacgagcgcg cgatgaatga
    24781 aattattttg ccgaccgtag cagggatgaa agcggagggg catgagttca ccggtttcct
    24841 gtacgcaggt ttgatgattg atcaaagcgg tgcaccctat acgattgagt ttaactgccg
    24901 tttcggcgat cctgaaaccc agccgattat gagccgactg aacagcgact tatcggattt
    24961 ggttgaagcg gcaatagacg gcagactcga tagcgtaacc gcggaatgga gcccgcaaac
    25021 tgccgtgggc gtggtactgg cggcgcaaaa ttaccccgaa acgcccaaaa aaggcgatgt
    25081 tatttccggt ctggatgctg ccaaccaagt aggcaaagtt ttccatgcag gtacaacggc
    25141 aaacgagaaa ggtgacgtat tgaccaacgg cggacgcgta ttgtgtgttg tgggattggg
    25201 cgacaatgtg gcgcaagcga aagctaaggc atatggcgtg ttggaaaaaa tcagtttcga
    25261 cgggatgcaa taccgtaaag acattgccga taaagcgatt aaccgttaac tagaaagagg
    25321 atgatgccgt ctgaagccaa gtatgactga tgtgcttcag acggtatttt atattatgtt
    25381 atttccgaga attatcgcag catcagcgca acgtaagtta agcgtttatt tgaaaaaagg
    25441 cggtttggtc gcttatccga ccgaatcttg ttatggcttg gggtgtcttc cgacattagc
    25501 caaagcactc ggtaagttgg cgcatttgaa aaaaagaccg caacacaagg gtatgattgt
    25561 catcgggaat caatttgaac aattgcagcc tttacttcaa atgccgtctg aaaatcttca
    25621 ggatatgctt agaaaagaat ggcctgcccc taaaacgttc ctattatccg ccaaatcctg
    25681 tgtcttacct gagttgcgcg ggaaacagcg cagcaagttg gcggtcaggg ttcccgccca
    25741 tgtaggtgcg cgccgcttgt gtcaggcttt gcaaacgcct ttggtctcga cttcgtgcaa
    25801 ccgcgcaggc aaacgcgcgt gtcggacgga gagggaagtc agaaggcaat tcggacgcga
    25861 tgtgtggatt gttggtggga gaatcggcag gcagaaatcg ccgagtcaga ttattgacgg
    25921 ggaaacaggt aaacgtttgc gttagatatg ggtacttgaa tgaaaacttt tttgcataca
    25981 caaagaaaaa attttggggc tgtactagat tagccctaaa tcccacacca atcctgtagg
    26041 atttttagct gccgggacag tgtgccgaag ttaaatcgaa attcgcattc tttcaagaac
    26101 agcgggaaag atttgcgatc gattccattg tattttcgca agacacgttt tgcctgattc
    26161 caaaaattct caatgccgtt aatgtggttc tgacggtctg caaa
//
LOCUS       DAXIEB010000015        23921 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000015 DAXIEB010000000
VERSION     DAXIEB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.015"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            76..777
                     /locus_tag="ACFOA3_000543"
     CDS             76..777
                     /locus_tag="ACFOA3_000543"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6897597.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            919..2712
                     /gene="lepA"
                     /locus_tag="ACFOA3_000544"
     CDS             919..2712
                     /gene="lepA"
                     /locus_tag="ACFOA3_000544"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6897598.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            2855..3874
                     /gene="lepB"
                     /locus_tag="ACFOA3_000545"
     CDS             2855..3874
                     /gene="lepB"
                     /locus_tag="ACFOA3_000545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6897599.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKVLTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFMCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(4093..4242)
                     /locus_tag="ACFOA3_000546"
     CDS             complement(4093..4242)
                     /locus_tag="ACFOA3_000546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897600.1"
                     /translation="MNWQAVKTGKTKHAGNGAERKMLSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(4288..5193)
                     /locus_tag="ACFOA3_000547"
     CDS             complement(4288..5193)
                     /locus_tag="ACFOA3_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6897601.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFNERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            complement(5389..5520)
                     /locus_tag="ACFOA3_000548"
     CDS             complement(5389..5520)
                     /locus_tag="ACFOA3_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897602.1"
                     /translation="MKPQTVQIVRQGEATLYWFLLIHYSTEPIHLVLQHLRESFSLS"
     gene            complement(5813..6745)
                     /gene="cysK"
                     /locus_tag="ACFOA3_000549"
     CDS             complement(5813..6745)
                     /gene="cysK"
                     /locus_tag="ACFOA3_000549"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6897603.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(6939..7544)
                     /locus_tag="ACFOA3_000550"
     CDS             complement(6939..7544)
                     /locus_tag="ACFOA3_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897604.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQAGPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMVLAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAALMQVSVLVLLLSEIGR"
     gene            complement(7541..8392)
                     /gene="dapF"
                     /locus_tag="ACFOA3_000551"
     CDS             complement(7541..8392)
                     /gene="dapF"
                     /locus_tag="ACFOA3_000551"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6897605.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFCYRIFNADGGEVEQCGNGARCFARFVADKGLTDKKEICVET
                     AKGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPVSGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWTRGSDVMMTGPAETVF
                     EGELAYS"
     gene            8529..8825
                     /locus_tag="ACFOA3_000552"
     CDS             8529..8825
                     /locus_tag="ACFOA3_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897606.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADSAVKGGFYKIIEIQGANRILIQDFNSTGNALGGFYVPDCRKSSLSPKNI"
     gene            8839..9507
                     /locus_tag="ACFOA3_000553"
     CDS             8839..9507
                     /locus_tag="ACFOA3_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6897607.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(9580..11700)
                     /gene="pnp"
                     /locus_tag="ACFOA3_000554"
     CDS             complement(9580..11700)
                     /gene="pnp"
                     /locus_tag="ACFOA3_000554"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6897608.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVREIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(11937..12800)
                     /locus_tag="ACFOA3_000555"
     CDS             complement(11937..12800)
                     /locus_tag="ACFOA3_000555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6897609.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERSIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDENGRLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            13000..13863
                     /gene="rfbD"
                     /locus_tag="ACFOA3_000556"
     CDS             13000..13863
                     /gene="rfbD"
                     /locus_tag="ACFOA3_000556"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6897610.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(14338..14571)
                     /locus_tag="ACFOA3_000557"
     CDS             complement(14338..14571)
                     /locus_tag="ACFOA3_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6897611.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(14737..14937)
                     /locus_tag="ACFOA3_000558"
     CDS             complement(14737..14937)
                     /locus_tag="ACFOA3_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6897612.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(15109..15984)
                     /gene="xerD"
                     /locus_tag="ACFOA3_000559"
     CDS             complement(15109..15984)
                     /gene="xerD"
                     /locus_tag="ACFOA3_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6897613.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(16036..16476)
                     /locus_tag="ACFOA3_000560"
     CDS             complement(16036..16476)
                     /locus_tag="ACFOA3_000560"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6897614.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            16797..17753
                     /locus_tag="ACFOA3_000561"
     CDS             16797..17753
                     /locus_tag="ACFOA3_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6897615.1"
                     /translation="MSIHTLKHLPSSLLLGLCLSLPSAHLFADNDILGQFDILGQFLE
                     QNMLTSSDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMP
                     IASISKLMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLM
                     SSENRATHALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSL
                     MVNAAAQYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGR
                     SMVVKANIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            17882..18175
                     /gene="hfq"
                     /locus_tag="ACFOA3_000562"
     CDS             17882..18175
                     /gene="hfq"
                     /locus_tag="ACFOA3_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6897616.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            18260..18826
                     /locus_tag="ACFOA3_000563"
     CDS             18260..18826
                     /locus_tag="ACFOA3_000563"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6897617.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            18880..19254
                     /locus_tag="ACFOA3_000564"
     CDS             18880..19254
                     /locus_tag="ACFOA3_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6897618.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            19264..19758
                     /gene="folK"
                     /locus_tag="ACFOA3_000565"
     CDS             19264..19758
                     /gene="folK"
                     /locus_tag="ACFOA3_000565"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6897619.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(19885..20364)
                     /locus_tag="ACFOA3_000566"
     CDS             complement(19885..20364)
                     /locus_tag="ACFOA3_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6897620.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(20583..21320)
                     /gene="ubiE"
                     /locus_tag="ACFOA3_000567"
     CDS             complement(20583..21320)
                     /gene="ubiE"
                     /locus_tag="ACFOA3_000567"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6897621.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(21363..21731)
                     /locus_tag="ACFOA3_000568"
     CDS             complement(21363..21731)
                     /locus_tag="ACFOA3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6897622.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            21851..23470
                     /gene="mnmC"
                     /locus_tag="ACFOA3_000569"
     CDS             21851..23470
                     /gene="mnmC"
                     /locus_tag="ACFOA3_000569"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6897623.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(23584..23701)
                     /locus_tag="ACFOA3_000570"
                     /pseudo
     CDS             complement(23584..23701)
                     /locus_tag="ACFOA3_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         6100 a         6906 c         5338 g         5577 t
ORIGIN      
        1 ccacctccac tcaatcctgc cgtgttagcg tgtataattc ggcattctat ccgaaattca
       61 cagcaggtca gcaatatgtc tttgaaaaca gtagccgtta tcggcgcaat ggaacaggaa
      121 atcaagcttt tgcgcgagat gatggaaaat atcaaagccg tctctttcgg cagattttcc
      181 gcctatgaag gcgaattggc gggaaaacgc atggtgcttg cattgagcgg catcggcaag
      241 gtcaacgcgg cggttgcaac ggcttggatt atccgtgaat tcgcagcgga ctgcgtcatc
      301 aacaccggca gcgcgggcgg tttgggcaag ggtttgaaag tcggcgacgt agtcatcggc
      361 acggaaaccg cacaccacga tgtcgacgta accgcattcg gctatgtttg gggacaagtg
      421 ccgcaactgc ccgccgtatt tgtttcagac ggcctcttgg tcggcaaagc aaaacaggcg
      481 gcggaagtgt ttgaaggcgc ggcggtagaa caaggcctga ttgtcagcgg cgaccgcttc
      541 gtccacagca gcgaaggcgt ggcggaaatc cgcaagcact tccccaaagt caaagcagtg
      601 gaaatggaag cggcggcgat tgcccaaacc tgtcatcagt tggaaacgcc tttcgtcatc
      661 atccgcgcgg tttccgattc ggcggacgaa aaggcagaca tcagctttga cgaatttttg
      721 aaaacggcgg cggcaagttc tgcaaaaatg gtggcagaaa tcgtcaaatc tttataaaac
      781 ctttatcaaa tcatgccaaa acagcggttt tccgttagaa tatcgggcta tttccacgtt
      841 gcgcccgata ttcggacgca ccgctgtttg ttcagacggc atttctaaaa cctaccgttc
      901 agcattaaaa acaactcaat gaaaaatatc cgaaatttct ccatcattgc ccacatcgac
      961 cacggcaaat cgacgcttgc cgaccgcttc atccaatatt gcggcggttt ggatttgcgc
     1021 gaaatgagta cgcaggtact cgattccatg gacatcgaaa aagagcgcgg cattaccatt
     1081 aaagcgcaaa ccgccgcgct caactacaaa gcacgcgacg ggcaggtgta tcaactcaac
     1141 ctgattgaca cgccgggaca cgtcgacttc tcttacgaag tttcccgttc gctgtcggct
     1201 tgcgaaggcg cgcttttggt cgttgacgcg tcgcagggcg tggaagcgca aaccgtagcg
     1261 aactgttaca ccgcgattga tttgggcgtg gaggttgtgc ctgttttgaa caaaatcgac
     1321 ctgccagccg ccgaccccga acgcgtggag caggaaattg aagacatcat cggcatcgat
     1381 gcggtcggcg cggtgcagtg ttccgccaaa agcggcatcg gcgtggaaga cgttttggaa
     1441 gaaatcgttg ccaaaattcc tgcgccgacc ggcgatgaaa acgcgccgct gcaagcggtt
     1501 attgttgatt catggtttga caactacgtc ggcgtagtta tgttgattcg tgtgaaaaac
     1561 ggcaccatca agctgaaaga caaagtgcgc tttatgagca ccaaggcgga aactcaggtt
     1621 gagcagctgg gcgtttttac gccgaaatca gttcaaaaac aagaactcaa agccggagaa
     1681 gtgggctttt tgattaccgg cgtgaaagaa ttgggacagg cgaaagtcgg cgatacggtt
     1741 actttggttg ccaaccctgc ctctgagccg ctgcccggct tccaagaggt gcaaagccaa
     1801 gtattcgcgg gtctctaccc cgtagaaagc cacgactatg aagccttgcg tgacgctttg
     1861 gaaaaattac agcttaacga cgcttcgcta aaattcgagc ctgaagtttc ccaagcattg
     1921 ggcttcggct tccgttgcgg cttcttgggt ctgttgcact tggaaatcgt gcaggaacgc
     1981 ttggagcgcg agttcgacat ggatttgatt accaccgcgc cgacggtggt ttatgaagtc
     2041 gtgttgaaaa gcggcgaaaa aatcgaagtc gaaaatccgt ccaaactgcc cgacatcggc
     2101 agcatcgaaa ccattctcga gccgattatt accgcgacca tcctcgtgcc gcaggaatac
     2161 gtcggcaacg tcatgacttt gtgtaaccaa aagcgcggcg ttcaggtcaa tatgcaatat
     2221 atgggccgcc aagtcatgct gacttacgat ttgcccatga acgaagtcgt catggacttc
     2281 ttcgacaaac tcaaatccac ttcgcgcggt tatgcttcgt tggactacca tttcaaagag
     2341 ttccaaccgt ctgatttgat taagctggat attatggtca acggcgaaaa agtcgatgcc
     2401 ttaagcctga ttgtgcaccg tcaaagcgcg gttcaccgag gccgcgagct ggcatcgaaa
     2461 atgcgcgagc tgattccgcg ccaaatgttc gacattgccg tccaagccgc catcggcagc
     2521 cagattatcg cccgcgaaaa cgtcaaagca ctgcgtaaaa acgtcttagc gaaatgttac
     2581 ggcggcgata ttacgcgtaa gaaaaaactg cttgaaaaac aaaaggcagg taaacgccgt
     2641 atgaaacaag tgggcaatgt ggaaattccg caaagcgcgt tcttggcgat tttgcaggtg
     2701 agcgacaaat aactctggca cacagggcgg cgcgtcggga acacccctca aaccgccctg
     2761 atttcagggg cacgcgcccg caaacaaatt ttgcaatttg ccgccttaaa acagttgcac
     2821 ggccgggcgg gtttttaagc attaaggaat aacaatgaac actatgctaa tgtcgggcgc
     2881 ggctgccgcg ctgcttgccg gcatcatcct ttatttcaaa agcgacaaaa agcggcagga
     2941 aaacggtgaa tggagttccg gcctcgaata cgcctatatc ctgacagcgg tcggcgtgtt
     3001 tgccgcttta tccctgttta tgagctttac cgccgttttc ctgattttcg ttgtattgtg
     3061 cggtacggct tggggggtat ataaataccg cctgaagact catcccgaaa tctcggaaag
     3121 cagccacttc ggcgattatt tcggcagttt cttccctacc gttttggtat tgttcctcat
     3181 ccggtcgttt atcgccgaac cgttccaaat cccgtccagc tcgatgcgcc cgggcctgat
     3241 caagggcgat ttcattttgg tcggcaaatt ttcctacggc ctgcgcgtac ccgttttaaa
     3301 caatatattt attcctacag gcaaaatcga acggggcgat gtcgttgttt ttaattatcc
     3361 tctgcagccg gagatgacct acatcaagcg tattgtcggc attccgggcg atgtggtcga
     3421 atatcgggat aaagttttga cggtaaatgg caaacccact tccgatattc ctaacggcac
     3481 ataccgttat cccgacgaca ccgacccttc cgaaatccac aacacggata tgttccgcag
     3541 cggtctggac ggcaaatcct tcaatattct gaaaaaagaa ggacagcctg ccgtttccct
     3601 gcccgtattg ggcaaatata cctccgatat tatgtctgaa aacggatatt ccatagagca
     3661 aagcggtttg gaacactgcc aatatgccga cgacggcagc ggtttcatgt gcaaagttcc
     3721 cgaaggacgc tatttcgcta tgggcgacaa ccgcgacaac agtgccgatt cgcgctactg
     3781 gggctttgtg gatgacaagc tggttgtcgg caaggcaatg ttcattttga tgaacttcgg
     3841 cgatttcggc agggccggta cggcaatccg ttaggccgga aaattacgcc ggaacatcaa
     3901 aaatgccgtc cgaaccctgt ttcagacggc atttttatca tcaaacgcct tgtttccctg
     3961 ccggcagcct cggggataat atagtggatt aaatttaatc cactatattt ccgtttgtgt
     4021 ggaaacccat cccattgaat aaagggcatt tcgtccgact gacagtgtgg gcggcatatg
     4081 gaaatacgga cattatttta gttgcattgc cattcctgcg gaggcggctt ttttgattct
     4141 gcccgccccg atgccgtctg aaagggcttc agacagcatt ttccgctccg cgccgttgcc
     4201 ggcgtgtttg gtcttgccgg ttttgactgc ctgccagttc aatcgtacgc aggtttgcca
     4261 cggcagaaaa tctgatgccg gacggagtca aggctcggac tgctgtttca gcgaaccgag
     4321 gtaaattgcc gaaagcgtca gtgccacccc tgcccactgc acgccttcaa tcggtttgcc
     4381 caatccgaaa taatcgatga acagagcggc aaccggttcg gacaaaagca gcagccccgt
     4441 cagcgacagc gaaagcagcg gaatcgcata ggcaaccatc gcccacgcaa agcactgcat
     4501 caccacgccg tacacaagca ccaaaccggc atctttccaa gtcgtcggat aaagcgcgcc
     4561 accgtccatc agtaatgccg gaacaaccag cgataccgcg ccacccaaac tcaaaatcat
     4621 cattgaaggg aaaagtgcca ccggctcgat ttcatgggtt ttgcggacga acaccatcga
     4681 cagggcgagc atcagcccgg acaccaaacc gctgacgaat ccccaaaccg cattaccgtt
     4741 gtagccgaat tccgcaccgg caatcatcgc cacgccagca actgccgata ttaagcccgc
     4801 ctttttcagc ccgctcaaac gttcgttaaa aaagaaaaca ccgattgccg acaagaaaaa
     4861 gatttgcagg ctgttgagca gggtggaaat acccggcccg accgcgtgta tgctttcgtg
     4921 ccacaacgcc aaatcgaaag cgagaaacac gcccgccgtc agggcatatc ggacggtttt
     4981 cctgtttttt gggaattttt gcctgaaaaa ccgtgctaaa aaccaaaata cgaacaccga
     5041 aatcagcaac cgccaaaatg cgattgcata cgaaccgacg gggacggatc tgacaatcag
     5101 actgcccaga ccgaacacca cgcaacccag caccagcaat ggtgcggcat attttttgcc
     5161 gttaccgacc acaatatccc ctcccttccc catacggctg ccgcgccgac ggcaagaatg
     5221 ccgccattcc gaaaatatgc cgtctgaagc ccaaagcccc ggctgcaccc ggacaaacgg
     5281 ctgactttac gggcaggcag gctattttcc gttggggcaa aaaaccgtcc tgccggataa
     5341 aacccgaata tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga
     5401 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat agtggattaa
     5461 caaaaaccag tacagcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcat
     5521 tgccttgtcc tgatttttgt taatccacta tatttgtact gtctgcggct tcgttgcctt
     5581 gtcctgattt ttgttaatcc actatatttg tactgtctgc ggcttcgttg ccttgtcctg
     5641 atttttgtta atccactata aaaaatattt ccctcaagcg atgcggcgac accaacacct
     5701 gattaaaaca caaccggcgt tttcggcata tatgcaaaaa cgcatttccc agcacggcgt
     5761 tacaccgata ccgaaaaatg cgtctgaatg tttcgacaat ccgattaaag cattatgcca
     5821 aatctgcaaa aagtggcgta gagagatagc gttcgccata agaaggcagc agcacgacta
     5881 tcagcttgcc ttcgttttca ggctgtttgg caagctgcaa cgcactccaa actgccgcac
     5941 cggaagaaat gcccaccaaa atgccttctt tttccgccat tgcgcgggcg gtttcaaaag
     6001 ccgcttcgtt cggcactttg gcgatgctgt cgtaaatttt ggtattcaaa acggtcggaa
     6061 taaagcctgc gccgatgcct tgaatcgggt gcgggccttt ttcgccgccg ctcaatacgg
     6121 gggaagcttc aggctcgacg gcaaccactt taacttcggg tttgtatttt ttcaacactt
     6181 cgcccacgcc ggtaatcgta ccgcccgtgc cgacgccggc aacgaagaca tcgactttgc
     6241 cgtccgtatc ccgccaaatt tcctcggcgg ttgttttgcg gtggacttcg gggtttgcct
     6301 cattgtcgaa ctggcgcggc ataaaataag tgtcgggatg cgcgtccacc aaggatttcg
     6361 ctttggcaat cgcgcccgcc ataccttcgg cggcaggggt cagaatcagc tccgcaccaa
     6421 acgcgcgcaa cagcattttg cgctccttac tcatgctttc cggcatggta atcgccagct
     6481 tgtagccgcg tgcggcacat accattgcca aaccgacacc cgtattgccg ctggttgctt
     6541 cgacaatgac ggtgtttttg ttgattttgc ccgctttttc ggcaccctca atcattgctt
     6601 cggcaatgcg gtctttgacg ctgctgcccg gattgaaaaa ttccagtttc acggcaacct
     6661 ctgccttcaa accttcggtc agacggttca gtttgaccaa aggcgtgttg ccgattagct
     6721 cggtgatgct gtttgcaatt ttcatcttct gctcctgaat aataaggttt cgacagtaag
     6781 gcgcaatccc cataggcatc ctgtccgatt gcaggggatt tgcgccgaaa cataaacaaa
     6841 tttcggatat ggctttatcc taaacctatg ccgcctaaaa aaccaatacc caaaatttct
     6901 ttctttataa ctttttgttc caatacggcc gcaccgtatt atcttccaat ttctgaaagc
     6961 agcagcacca gtaccgagac ctgcatcaac gccgccgcca acacgccgag gcgcagctgc
     7021 gaaccgccga acgttgacgg cggctgccga tctataccgc agcggtggac ataagcaagc
     7081 accatcagca caaactgcaa cacaaaccaa taaaccggct tcagcgcata aagtttgagc
     7141 agtgccgcaa acacaaacac ttgaaccgtt cccgaaaagc aataatgcac caacgcacat
     7201 attccgacaa aagcaagcgt catcgataca ttactgacgg caaacagccc gagcagcgga
     7261 tgttcggggc ctgcctgcca tccgtttcca tctgttttcc ggttccaatg cccgatgaaa
     7321 aaaaatatgc tgcccaaagt caggtaaaaa tgggggatga acaagggcgc ggcgcgggtc
     7381 attccccata tgccgggcat cagctttgcc cccaaaaccg atatgccgag ccacaacgca
     7441 atactcgccc aaaaccattg caacattcct gcgcggaata tcagaaacag aaaaaccgcc
     7501 gcatacagta aggcagacaa aaaatccaaa tgcagcaaaa tcatgaatac gccaactcgc
     7561 cttcaaacac ggtttccgca gggccggtca tcatcacatc gctgccgcgg gtccattcga
     7621 tatataaagt cccgcccggc aaaaccacct ctaccgtttt cccttcatcc aacagcccca
     7681 gacggatacc cgccaccaca gccgcacacg cgcccgtgcc gcacgcttgg gtttcgccca
     7741 cgccgcgctc gaacacgcgc aaacgaatcg cggttcggcc gacaacctgc ataaagccga
     7801 cattgacgcg ttcgggaaac tgcctgtgcg gttcgataag cgaaccggtt tcgcgcaccg
     7861 gcgcgcattc tacgtcatcg accacaatca ccgcatgggg gttgcccata ttgacgcagc
     7921 tgacaggctg aatgccggat tcgagatgca ccccgtaaat acaggcatca tccccctcgc
     7981 ccgatacggg gacaaacggt atttcagacg gcataaactt cggtttgccc atattgaccg
     8041 taaccatacc gttatcggac agtttcggaa aaataacgcc cttcgccgtt tcaacgcaaa
     8101 tttctttctt atcggtcaga cccttgtctg cgacaaaacg ggcaaaacaa cgcgctccgt
     8161 tgccgcattg ctcgacctcg ccgccgtcgg cattgaaaat acggtaacag aaatccacac
     8221 cttcggtttc cgaacgcccg accaccaaaa gctggtcgaa gcccacgcct cggaagcggt
     8281 ccgcccatgc cgcaatcggc gcgtcctcgg gggtaaaatc ctgactgacc gcgtcaatca
     8341 ccataaaatc gttgcccaaa ccgtgcattt tagtaaattt caaagtgttc atattattct
     8401 gaaatcccgt attgtctgtt gcttccgaat tccgaaatcc ccgtgccgaa gggaaatgct
     8461 tccatctccc gccgcctgag gattaaaatc agccattata ctgtttgtta ccccttacaa
     8521 gaaacaccat gttccgcata ttactgttgc ttttcccttg tgcaatcctt gccgcctgca
     8581 cccgaaacat accggcaaac ccggccgttt caaattgcgc gcatcttcaa aaccgcacca
     8641 tcctgtattt cgacgcacac ggcaaacccg ccgacagcgc agtaaagggc ggattttaca
     8701 aaattatcga aatacagggt gcaaaccgca tccttattca agactttaac agcacgggca
     8761 atgccctcgg cggattctat gtgccggact gccggaaatc atccctttca ccgaaaaata
     8821 tctgacttca aactgcctat gaaccacgac atcactttcc tcaccctgtt cctactcggt
     8881 ttcttcggcg gaacgcactg catcggtatg tgcggcggat taagcagcgc gtttgcgctc
     8941 caactccccc cgcatatcaa ccgcttttgg ctgatcctgc tgcttaacac aggacgggta
     9001 agcagctata cggcaatcgg cctgatactc ggattaatcg gacaggtcgg cgtttcactc
     9061 gaccaaaccc gcgtcctgca gaatatttta tacacggccg ccaacctcct gctgctcttt
     9121 ttaggcttat acttgagcgg tatttcttcc ttggcggcaa aaatcgagaa aatcggcaaa
     9181 ccgatatggc ggaacctgaa cccgatactc aaccggctgt tacccataaa atccataccc
     9241 gcctgccttg cggtcggaat attatggggc tggctgccgt gcggactggt ttacagcgcg
     9301 tcgctttacg cgctgggaag cggtagtgcg gcaacgggcg ggttatatat gcttgccttt
     9361 gcactgggta cgctgcccaa tcttttagca atcggcattt tttccctgca actgaaaaaa
     9421 atcatgcaaa accgatatat ccgcctgtgt acgggattat ccgtatcatt atgggcatta
     9481 tggaaacttg ccgtcctgtg gctgtaagcc ccattcattc ccgatacccg taaaaaatac
     9541 cgtctgaaac ctgttcagac ggcactttca ccctaagcgt tactcggcgg cattttcctc
     9601 acgggcaggc gcgtccagca gggctttgat ggacagacgg acacggcctc tgtcgtccac
     9661 ttccaatgct ttcacgttca ccacctgacc gacttgcagg tagtcgccga cattgcgtac
     9721 gcgctcgtgg gcgatttggc tgatgtgtac caaaccgtct ttgcccggca tcacactgac
     9781 aatcgcgccg acattgttgt cgaggatttt caccacagtg ccttcgtaca ctttgcccac
     9841 ttccacttcg gcagtaatct gctcgatgcg ttttttcgcc gcatcgccgg cttcttgagt
     9901 ggttgcggca atggtaatcg taccgtcttc ggcaatattg atttccgtac cggtttcagc
     9961 ggtaatcgca cggatggttt caccgccctt accgataact tcgcggattt tgtcttggtt
    10021 gattttcatc gtgaacaagc gtggcgcgtg tgcggacagc tcttgcgggc ccgcaacggc
    10081 ggctttcatc tgatccaaga tgtgcagacg cgcttctttg gcctgtgcca aagcgatttg
    10141 catgatttct ttggtaatac cttggatttt gatgtccatt tgcagcgcgg ttacaccttc
    10201 ggtcgtaccg gctactttaa agtccatatc gcccaagtgg tcttcgtcgc ccaagatgtc
    10261 ggtcaggacg gcaaatttgt tgccttccag aatcagaccc atagcgatac cggcaacgtg
    10321 tgctttcaaa ggcacgccgg cggacagcag gctcaggcag ccgccgcaga cggaagccat
    10381 agaggaagag ccgttggatt cggtaatttc ggagaccacg cgcatggtgt agctgaaatc
    10441 ttcaggtttc ggcaatacgg ccaacaatgc acgtttagcc aaacggccgt gaccgatttc
    10501 acgacgtttt ggcgcaccta cgcgacccac ttcgccggta gagtatggag gaaagttgta
    10561 gtgcagcatg aagcggtcgg tgtattcacc ggacagcgca tcaatgattt gctcgtcgcg
    10621 tgaagtaccc aaagttgcaa ccgccaaagc ttgagtttcg ccacgggtaa acaatgcaga
    10681 accgtgcgtg cgcggcaata cgccggtttg aatgttcagc ggacgaacag tgcgggtgtc
    10741 gcgaccatca atacgcggtt ggccgtctaa aatttggctg cggacaacat cggcttccaa
    10801 gtgtttgaaa atgcctttga tttcgttggc tgccaaagtg tcggtttcct cggtaatcaa
    10861 ggcttctttt acagcactcc aagcttcgtc caatttggca gaacgcgctt gtttttgacg
    10921 gattttgaat gcttctttaa tggtttcacc ggcaatttcg cgtacttttg caaccaattc
    10981 ttcattggtt tcaggtgctt tccaatccca aagttccgga ttgacttcgt cggcaaattc
    11041 attgattgca ttgatggcaa cctgcatttg atcgtggccg taaaccaccg cgcccagcat
    11101 cacgtcttcg ggcaggattt tggcttcgga ttccaccatc aacacggctt ttgaagtacc
    11161 ggcgaccacc aagtccaatt gcgatttcgc caattcggct ttagtcgggt tcaaaacgta
    11221 cacgccgttt acataaccga cgcgtgccgc gccgatcggg ccggcaaacg gtacgccgct
    11281 caacaccagc gcggcagatg cacccaacat tgcaggaata tcagaatcga tttcaggatc
    11341 gacggacacg accatcgcta cgatttggat gtcgtggtag aaaccttcag ggaacagcgg
    11401 acgaatcgga cggtcgatca gacggctggt caggatttct ttttcgcttt gtttgccttc
    11461 gcgtttgaag aaaccgccgg gaattttgcc tgcggcgtaa gtgcgctcga ggtaatcgac
    11521 agtcagaggg aagaagtctt ggccttcttt cacttctttg ttggtggtaa cggcaaccaa
    11581 aaccacggtg tcgcccatag agactttaac ggcagcggcg gcttggcggg caatttcgcc
    11641 ggtttccaaa gtaacggtgt ggttgccgta ttggaaagtt ttaacgtgtt tgtcgaacat
    11701 tattgttcct ttcaaaatac cgcactgcta aaacactaat aatgcacact aaaatccgaa
    11761 tgtgcatagt tagggtttca gaccgtgcgg caggttgtaa acaagctttc agacgacatt
    11821 tcggacgctg aaagcaattg cggattataa aggcaaccat ccttaaaatc cagtattgat
    11881 acaataaaaa ggtcgtctga accatatttc tttcagacgg ccttcaaact taaaaatcaa
    11941 tcctgagtca gcaaagtcaa tgcttcccga tacttctcga cagttttctg aatcacatcg
    12001 gcaggcactt tcggcgcagg ggcttttttg ttccaaccgc tttgttccag ccagtcgcgg
    12061 acgaattgtt tgtcaaaaga cggcggattg gtgccgactt catattgatc cgccggccag
    12121 aagcggctcg aatcgggagt caatacctca tccatcagcg tcagcctacc gttctcatcc
    12181 aaaccgaatt caaatttggt atcgcaaatg atgatgccgc gcgatttagc atattccgcc
    12241 gcttctgtat aaagctgaat cgctttggcg cgcacttccg ccgccaattc ttttccgata
    12301 ctgcgttcgc attcttcaaa gctgatgttt tcatcgtgat cgccgactgc ggctttggtt
    12361 gagggcgtaa aaatcacttc aggcagttgt tgcgcttcct gcataccttc gggcagccga
    12421 ataccgcaaa ccgagccggt tttttgataa tctttccaac cgctgcctgc cagataaccg
    12481 cgcacaatcg cctctacttt caccggagtg agctttttag ccacgacggc gcgtttttct
    12541 aaaactttgg cttcgttttc aggcaaaaca tcgtaaaccg tttgaccggt aaagtggttg
    12601 ggcataatat gcgccagttt tttaaaccaa aaattggaaa tctgcgtcag aatctcccct
    12661 ttgctcggaa tcgggtcgtc caaaatcaca tcaaacgcgg acaggcggtc ggaagcgacc
    12721 atcagcatac gtttatcgtc gatttcatat aaatcgcgca cttttccaga atagatcttt
    12781 accaaaccaa tctcactcat ttcgcccccc cctgaaaata tcttgaaaat accgacccga
    12841 cacccgacag gtttgaatcg caaaccgata ttctagccga agtcggcgca aaacaatacc
    12901 catggcacaa aaagccaacc cgtcaaccgt cggcaaagtt ttggcactat aataccgaca
    12961 gcaagtccta caatacactt ttaccaaagg aaatacctca tgagaatcct attgacaggc
    13021 tcgaaaagcc aactggcacg ctgcctgcgc gaccgtcttc cggaagactg ggaaaccatt
    13081 gcgacggatt ccgcatccct agacattacc gatgccgatg ccgtctgcaa catggtcaaa
    13141 agtttccaac ccgacgccat tgtcaacacg gctgcctata ctgccgtcga caaagcggaa
    13201 ggcgatgcgg cagcggcatt tgccgtcaat gcttccgccg tttacaacct tgccttggca
    13261 gcacatcgcg cccatgcccg attcatccac atctcaaccg actatgtatt tgacggtaaa
    13321 gggaaaagac cctatcagga aagcgacttt accaatcctt ccaatgtata cggacaatcc
    13381 aaaaccgcag gcgagctgct cgcactgtct gccaatcccg acagccttat cctgcggact
    13441 tcttggctgt ttagcgaata cggggacaac tttgtccgca cgatgctgaa ccttgcgcgg
    13501 gaacgttccc cgctgtccgc cgtccacaac caaatcggct gcccgaccta tgccggcgac
    13561 ttgtccgcca ccatcatccg cctgttgcag cactccaatc ccgttcgcgg catttaccac
    13621 tacgccggcg gcaaatccgt atcttggtac gaatttgtcc aacatatttt ccaagcggca
    13681 tcgcaacagc agacatcctt ccccattccc gaattgactg ccgtttcaga caaggaatat
    13741 ccgaccgccg cccccaggcc cgcatacagc attttggact gccgcaaaat cgaaaacgac
    13801 ttcggcatca aaccgtcaga ctggcaaaaa gcccttgcgc aggtcgtttc caagctgctc
    13861 tgatgccgcc cgcctctccg tttccgccgt caagcaccgc cttggcggtt ttcttatata
    13921 gtggattaac aaaaaccagt acggcgttgc ctcgccttgc cgtactattt gtactgtctg
    13981 cggcttcgtc gccttgtcct gatttttgtt aatccactat aaaatctacc gattacacaa
    14041 acacatatca tctttacaca atcatgcttc catcaacagt aaaacatgat atgattgcca
    14101 acaataacat ctcacaataa attttctaat ttttattgaa aaaatcaata aataagaatt
    14161 ctcccaaccg acaaataaaa taaagaaagg gttaatatgc aacatcgaag aagactagca
    14221 atttaccaag catccaaacg tgcttccttt accggcaggt catccgcccc gcaaaaacgt
    14281 aaagaacgtt aatttgaaaa aaatgccgtc tgaagtcctg cttcagacgg cattttttta
    14341 ccgttcgaga aactgtttca acctgtcctc atccaaaaac cagtgacaga tttcctcatc
    14401 tcccgccaac aaaacgggaa ccagctcatt gtatttttct tccaaaacag gattttcatc
    14461 cacatcgacc acttccagcc cgaacccgta ttcattttga aaaggtttga gtgcatcgcg
    14521 cattttgtgg cacaagctgc aatattcacg aaacatcaag gtcaatttca tccgcgtttc
    14581 cttatctgtc aatttgcaca cgccaaaacc ttagacgcag cagaatcatg gtctatttgg
    14641 gaaaaaacaa tgttttcaag gaagatgata ctcaagtcct gccaaaaaca gcaaaaatgc
    14701 cgtctgaaca gttcagacgg catttccaaa accgttttac gcttgaacat tgatacccgc
    14761 cgtaaccgtc ggttgcgcat tgtgcgccgt cagaaacgaa gcggcaagct ccccacagca
    14821 gccgcagcag ctcgctacgc ccaattgcga ctgcaaatcg cccattgtgg tcgcgccggc
    14881 ggcgatggtt tccttgattt gatggtcggt aacggcattg cagatgcaga caaacatttt
    14941 gtgctccgtg tgttctcaaa ctatcgtacc gatagcggct tttattatcg tatgcaaata
    15001 taaataaaaa cggttcgcat tgcaaggtcg gcatacacgg tttgtcttgg taatttttta
    15061 tcaagttttc atttccgatt gatttcttat taaaccgaag cagaagcctc agtttcggga
    15121 atggtgttcc ttcactacac cctgcaacca tacattggca acatgggtgt aaatctgcgt
    15181 cgtattcaaa tcggcatgtc ccaacatatc ctgaaccacg cgcaaatcca atccgtgctg
    15241 caccaagtgc gtggcaaagg cgtggcgcag gctgtgcggg ctgatgtgcc cgatgcccgc
    15301 cctacctgca tattctttga caatcatcca tgccaactga cgagaaatgc ccgtcttttt
    15361 ctgactgaca aacaatgcgt cgcaattcct gcctttcagc agaagtgggc gtgcctccgt
    15421 ataatagcgt tccacccaat acgccgactc ctgccccatc gggaccatcc tctgcttatc
    15481 gcccttgccc agcgcggtaa tacagcccct gtccaaatcc acattgccga agttcagccc
    15541 gaccgcctcg ctgacgcgca agccggtcgc gtacatcaat tcgagcagtg ccttgtcccg
    15601 caaaccgtgt ggcgtatcag tatccggagt ggcaagcagt cgggaaatct gctgctcggt
    15661 gatcagggtc ggaatattct tgtcgatttt gggcggtttc agcaaacggg tgggattgtc
    15721 cgtccttatg ccttcacgct ccatccatat atacaggcgt ttgcacgccg ataatgcgcg
    15781 cgcctgcgaa ctccgttgct ctccgtcaac ataaaccgcc gccgccaaat ccgcttcgtc
    15841 cgcatccttc agcgttctgc ccgattggga caggcggcgg gcgatttttt ccaaatcgcg
    15901 ccggtaaccg tttaaagtat tctgattgag ccgcctgtcc aaccacagcg tttcaagcag
    15961 cctgtcgatc aaaccttctt ccataccgtt ccaaacaaat gccgtctgaa tcttcttcag
    16021 gcggcatggt tcacattatc gggaaagcgt ttccaacact tcctgtgcat gacccgccac
    16081 tttgactttc cgccattcat gggcgatttc tccatcctta ttcaagacga acgtactgcg
    16141 ctcgatacct aacgactctt tcccgtacag tttcttcaat ttgatgacat caaacaggcg
    16201 gcacactgtt tcatccttgt cgctcaacag ctcgaaccgg aaaccctgct tggcgcaaaa
    16261 attctgatgc gcctttacgc cgtcgcggga aataccgacc acggtataac ccaatgcctc
    16321 aaactgttcc aaacgcgcat tgaaatccaa gccttccgtc gtacagccag gcgtactgtc
    16381 tttcggataa aaatacacga ccaaaggcag atgttctgcc gaatgaaaat ccgcaccgct
    16441 gctcgaaggc agggtaaatt catatttcac atccatagtc ctactcccga tattcccatt
    16501 attcaaaacg gcacgcagac gcaccgccgc aattgccaaa ccaaccccga ttctaccgcc
    16561 ccaaaggaca aggattcaac cgccggaaac atccaaaccg acacacgacg gcatgaaaaa
    16621 tatccatgtc aaaccacaaa atatgttccg atttaaaaac agaatgttat aaaacccaat
    16681 ccccaaaaca caacaagacc gcccgcctac gggcagtctc ctgtcagacg acatacttta
    16741 cagatggctg ttttttcaac aaaataacgc caatactcaa aaatatggaa tcaaaaatgt
    16801 ccatccatac tctgaaacac ctgccctcat cgctgctgct cggtctctgc ctttccctgc
    16861 cgtcagccca cctttttgcc gacaacgaca ttttagggca atttgacatt ttagggcaat
    16921 ttttagaaca gaacatgctt acctcctccg atccgataga aatattcgcc gaaagcacga
    16981 tacaccccac caacacccaa gccattacag gcggtctgat tctctcctca cagtctgccc
    17041 tggtcgtcaa caacaaaacc ggacagatac tgtatcagaa aaacgccgac aggattatgc
    17101 ccatcgcctc catttccaaa ctgatgagcg cgatggtcgt tttggatgca aacttggaca
    17161 tgaacgaaac cgttaccatt acgcccgacg aaatcgaccg catcaaaggg accggcagcc
    17221 gtcttgccat aggtacggca cttacacgca aagaactgct gcacctgagc ctgatgagca
    17281 gcgaaaaccg cgccacccat gcattgggca gaacctaccc cggcggcatg ggcgcatttg
    17341 tcgccgctat gaaccgcaaa gcccaaagcc tgggcatgta cagcagccgc ttttacgaac
    17401 cgaccggact caacttccaa aacgtttcca ccgccaaaga cctgagcctt atggtcaacg
    17461 ccgccgccca atatccgcaa atccgcacca actcgacttc caactacgcc tcggtacaga
    17521 ccaaaaacgg gcagcagaac tacaaaaact ccaatgcgct ggtcagggaa ggcatgtgga
    17581 acatcgaatt gcagaaaacc ggctacatac gcgaagcagg caggtctatg gttgtcaaag
    17641 ccaacattca aaatcaaccc gttaccatcg tattgctgaa ctcgcccaca tccgccacac
    17701 gcgtcaacga cgcccgcaaa atcgaatcgt ggatgctgca gcaacgctcc tgacatacaa
    17761 atgcccggcg gaaaaccgcc caaacacaaa aaaaattggt aatttttcct attcaaggct
    17821 acaattcgac acgcacaggg cgtctgcccg atttcacaat cataatagcg gagttaaaaa
    17881 tatgacagct aaaggacaaa tgttgcaaga tcccttcctg aacgccctgc gtaaagagca
    17941 tgttccggtt tcgatttact tagttaacgg tatcaaattg caaggtcagg ttgagtcttt
    18001 cgatcaatac gttgttctcc tgagaaacac ttccgtcacc caaatggttt acaaacacgc
    18061 catttccacc atcgtaccgg cacgctccgt caacctacaa catgaaaaca gaccccaagc
    18121 cgcaccgact tcgaccctcg tccaagtgga aaccgtccag cagcctgccg aataatccgc
    18181 acgaagcatg acgtgtcata tctttcaata ccttaccgga caacggtaag gtatttttat
    18241 tttcagacag catttaaaaa tgttattgca aaacatcctt ccattcgccc attgcctttt
    18301 gcggaaggca cttcccgaag gtggcaatgc tttggacggc accgccggca acggacacga
    18361 cacccttttc ctcgcacaaa ccgcaggcat ccgggggaaa gtgtgggcat tcgacatcca
    18421 gccgcaagcc ctgagccgca ctcgatgccg tctgaaagaa gcgagatacg agaatgtgca
    18481 actcatctta gacggacacg aaaacctgaa acaatatgtt tcagaaccac taaatgccgc
    18541 cattttcaat ttcggctggc tgcctggcgg ggacaaaagc cttaccaccc gcacggaaac
    18601 cagcattgcc gccctttctg ccaccttatc cctactgaaa gaaaacggta tgcttattgc
    18661 cgtcctctat ccgggacacg aaaacggcaa acaggaggca gaagcaatcg aacaatgggc
    18721 aaaaaacctg cctcaagaac agtttgccgt tttgagttac ggctttacca accggaaaaa
    18781 caacccgccc tatctgttgg catttgaaaa gttacgtcaa aaataaatgt ttgcggtaaa
    18841 ataagccgat tctaaccctt attttgatac gagccgacca tgcaatatct gattgtcaaa
    18901 tacagccatc aaatcttcgt taccatcacc attttggtat tcaacatccg tgttttccta
    18961 ctttggaaaa atccagaaaa gcccttggtc ggcttttgga aggcactgcc ccaccttaac
    19021 gacaccatgc tgctgtttac gggattgtgg ctgatgaaaa ttacccattt ctccccgttc
    19081 aacgcgcctt ggctcggtac aaaaatcctg cttctgctcg cctatatcgc attgggtatg
    19141 atgatgatgc gcgcccgtcc gcgttcgacc aagttctaca ctgtttacct gctcgccatg
    19201 tgttgcgtcg cctgcatcgt ttaccttgcc aaaaccaaag tcctgccttt ctgaaacacc
    19261 gttatgaaca acagacattt tgccgtcatc gccctgggca gtaatcttga aaaccctgcc
    19321 caacaggtac gcgccgcatt ggacacgctg tcgtcccatc ctgacatccg gcttgaacag
    19381 gcttcctcac tgtatatgac cgcacccgtc ggttacgaca atcagcccga tttcatcaat
    19441 gccgtctgca ccgtttccac cactctggac ggcattgccc tgcttgccga actcaaccgt
    19501 atcgaagccg atttcggacg cgaacgcagc ttccgcaacg cgccgcgcac attggatttg
    19561 gacattatcg actttgacgg catctccagc gacgaccccc gactcaccct gccgcatccg
    19621 cgcgcgcacg aacgcagttt cgtcatacgc cctttggcag aaatcctccc tgattttatt
    19681 ttgggaaaac acggaaaggt tgccgaattg tcaaaacggc tgggcaatca aggcatccgt
    19741 cttttaccgg ataagtaatt ccgcacgcgg atgccgtctg aatgccggtt tcagacggca
    19801 tttttccttt gccgccaaca cgcgtgcaaa aaaatcgccc cttggaaaag gggcgcaaaa
    19861 ggaacacaaa ccactaccaa aactttaaat ctgaaacact gcctgccgca tactgtatcc
    19921 gacaggatat aaagccctca ctaaatcgtt tcgagaaatc caaacttctt catcgccgac
    19981 agaaaatctg cctttctccg gcaccagctc caacaaaaac ggttgaaccg ccgtatgcag
    20041 cctgtcctta caccgcccca gctttctgga caacgcggac agggcgcgtt tgtaggcatt
    20101 atccttgcag tcaagctccc gcgcactgcc gacccagctc aaacgaaggt tgcggctttc
    20161 atcctcaatc agcaaaatgc ctgttttgac ttcccccacc tcgggctcgc atgaaagcgc
    20221 aatataatat ttgccgtcga tgttgacata cgccgcttca tgcacctgac agttgcagcc
    20281 ggaagtggca agcagcgaat aaacagaatc ccgatgcagt ttcaaaatat caagggaaat
    20341 ctgtttgaaa atactttccg gcattttccc tcctttcgtc tgctgcgttg tttactggat
    20401 tgactgcatt atacagacat ctcccaatct tgcaaatgat aattgttcct attttttaaa
    20461 aagcttataa attattgata tatatgtaaa ttaattttta agatttaaaa cctttgtctc
    20521 aagaaacaca aaaatgccgt ctgaaacgtg ttcagacggc attggctgca caggcagtcc
    20581 gtttaaaatt tcacgccctt atgcagcgcg acgatgcccg cactcatatt gtgataatcc
    20641 acgctgtcaa agcctgcatc cagcatcatc tgtttcaaag tttcctgatc ggggtgcata
    20701 cggatggatt cggcaagata ttgataactc tccgcatctt tggcaatcag cctgcccatg
    20761 accggcagca gcttgaacga atagaaatca tacgcgcctt ccaaaggttt gtagattttg
    20821 gaaaactcca acaccagcaa cgtgccgccc ggtttcaaaa cacggtacat ctctttcagc
    20881 gcggcatctt tatgcgtcat gttccgcaag ccgaacgcca cggaaaccaa gttgaaataa
    20941 ttgtcgggga aaggcagttt ttccgcatcg gcaagcgata cgggcaaaat catgccttcg
    21001 ttcaacagac ggtcgcgccc gacggtcagc atagaggaat taatatcggt cagccaaacc
    21061 tcgccttcct tgccgacccg tttcgcccaa ccgcgcgaca aatcgcccgt accgcccgca
    21121 atgtccaaca ctttatcgcc ttttttcagg tgcgccgtgt tgatggtgaa atgcttccac
    21181 accctgtgca gccctgccga catcacatcg ttcataatgt cgtagttttt ggcgacggag
    21241 tggaacactt ccgccacttt tccggctttc tcatcttcgt tgaccgtact gaatccgaaa
    21301 tgcgtttttt gtccgcccat cttctgacca cctccctgat gccgtcctcc ggcatcccaa
    21361 acttaacaat ctgattgctt ttcaaaacgc gacgcaccag ccgccgccaa tttgtccaaa
    21421 tattcctgcc acatcgcatc gtatccgtat gccagtctgt gcagatacgc ccaatcgtaa
    21481 agaccgctgt cgtgcccgtc cgaaaaactg attttcagcg catactgtcc gacaggctgc
    21541 aaatccgtga tttggacatc cgccttgccg gtctgcaaaa catcctgtcc cacgccgtgt
    21601 ccgcgcactt ccgcactcgg cgaatagacg cgtaaaaatt cggcaggcag atttttaggt
    21661 tcgtccccat aagtcaaaac caacacggta cggtttttca acaaacggat ttcaacagga
    21721 atattattca aaaaacacct cggttcggca tacaatatcc ggcaggcgca ggcgtgcctt
    21781 caaacggcgc gtattttaac agctttatcc cgataccgat attcaaaccc acatttcaga
    21841 cggcattgat atggacaatc tcgtatggga cggcattccc gacatccgca cactcgacca
    21901 aaccatccgc aaacacgcac acccgctcaa cctgattgtc tgcctccccg ataatcagat
    21961 tcccgatttt caaaccgcac aagatgcttc ggacgcggaa tgccgtctga agcaccgttt
    22021 ggatcaggca acgcagtgcc tccagttcga cagcatcaac ctcatcgaac acatcctgcc
    22081 cgatgtccgc ttctggctgg ttcccccttc acgcacccgc cgcctgcacg aacacttcca
    22141 ccacatttcc tggcagaccg aagccatccc gcaaaccgaa agtaagcccg acaaaccctg
    22201 gtttgcactt ccacaaacat ccgaacggca aaaaccggaa cacatcctcg ttatcggagc
    22261 gggcatatcc ggcgcggcaa ccgcccacgc cttagcatca tacggcattt ccgttaccgt
    22321 attggaagcc cgaaaagccg cccaagccgc cagcggcaac cgccaagggc tgctctacgc
    22381 caaaatctcg ccacacaata ccgaacaaac cgaacttttg ctcaccggct acggctacac
    22441 caaacgcctg ctcggacaca tcctgcccga atccgaaacc tggggcggca acggcatcat
    22501 ccacctcaat tacagccgca ccgaacaaca acgcaatcac gaattgggtt tgcaaaaaca
    22561 ccataaccac ctctaccgca gcatcacgca ggcagaagcc gaaaaaatcg ccggtatccc
    22621 gctgaacacg ccctacgccg aaccattatg cggactgttt tggcagcacg gcgtatggct
    22681 caatccaccc gcattcgtcc gcgccctcct ccgccatccg ctgattggac tacacgaaga
    22741 cacaccgtta accggtattt cccacgacgg agaaaagtgg attgcaagca cgccaaacgg
    22801 catcttttct gccacacaca tcgtctactg caccggcgcg cacagcccct gcctgcccga
    22861 aaccaacctc gccgccctgc ccctcaggca aatacgcgga caaaccggcc tcacaccgtc
    22921 caccccgttt tccgaacaac tgcgttgcgc cgtttcaggc gaaagctaca tcagcccgtc
    22981 gtggcacgga ctgcactgct acggcgcgag ttttattccc aacagcagcc ataccggatg
    23041 gaacgaagcc gaagaagcct caaaccgcca agcattggca caccttaacc ccgcccttgc
    23101 cgaatcattg tttgccgcca acccaaaccc ccaaaaatac caagggcacg ccgccatacg
    23161 ctgcgacagc cccgaccacc ttcccctagt cggcgcactc ggcgacattg ccgccatgcg
    23221 gcagacctac accaaactcg cgctggacaa aaactaccgc atcgacaccc catgcccata
    23281 cctgcctaat gcctacgtca acaccgccca cggcacacgc gggcttgcca ccgcccccat
    23341 ctgcgccgcc gccgttgcag ccgaaatcct aggcttgccc catcccctct caaaacgcct
    23401 gcgccacgcc ctacacccca accgcaccat catccgcgcc atcgtcagaa ggaaggatct
    23461 aaccccttaa gcctttgccg catgccgtcc cgaccacaga aaatcatatt caaataaaca
    23521 taccaatcct gacggtaaaa tatcgaaaac acccataacg ttttcttccc aaaataagga
    23581 gtaaggaatg cctaacaaat tgcctttgct gtccaagatg aatgttccgt aacattcgaa
    23641 gacttcctta accctgccag ccgtttcggt ttctgtaccc cctgttagat aaggaaggaa
    23701 tgaaaaagcc ctgttgattg tatgaagtaa agggcgttaa tttttgttaa tcttcccttc
    23761 tcaggaactc aatatataag gatgttaatt tttatttccc ttctcgagga ctcaatatag
    23821 tggattaaca aaaaccagta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc
    23881 ggcttcatcg ccttgtcctg atttttgtta atccactata g
//
LOCUS       DAXIEB010000016        23672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000016 DAXIEB010000000
VERSION     DAXIEB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.016"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(93..932)
                     /locus_tag="ACFOA3_000571"
     CDS             complement(93..932)
                     /locus_tag="ACFOA3_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6897624.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="ACFOA3_000572"
     tRNA            1217..1292
                     /locus_tag="ACFOA3_000572"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1349..2551)
                     /locus_tag="ACFOA3_000573"
     CDS             complement(1349..2551)
                     /locus_tag="ACFOA3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6897625.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLKLRV"
     gene            complement(2572..3660)
                     /gene="trmA"
                     /locus_tag="ACFOA3_000574"
     CDS             complement(2572..3660)
                     /gene="trmA"
                     /locus_tag="ACFOA3_000574"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6897626.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3803..4903
                     /gene="aroC"
                     /locus_tag="ACFOA3_000575"
     CDS             3803..4903
                     /gene="aroC"
                     /locus_tag="ACFOA3_000575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6897627.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4994..5419
                     /locus_tag="ACFOA3_000576"
     CDS             4994..5419
                     /locus_tag="ACFOA3_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6897628.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5493..7478)
                     /gene="parE"
                     /locus_tag="ACFOA3_000577"
     CDS             complement(5493..7478)
                     /gene="parE"
                     /locus_tag="ACFOA3_000577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6897629.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTRTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKSARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7545..8093)
                     /locus_tag="ACFOA3_000578"
     CDS             complement(7545..8093)
                     /locus_tag="ACFOA3_000578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6897630.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQSSDNR"
     gene            8235..9530
                     /gene="serS"
                     /locus_tag="ACFOA3_000579"
     CDS             8235..9530
                     /gene="serS"
                     /locus_tag="ACFOA3_000579"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6897631.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYEALEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9608..10606)
                     /locus_tag="ACFOA3_000580"
     CDS             complement(9608..10606)
                     /locus_tag="ACFOA3_000580"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6897632.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDVKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="ACFOA3_000581"
     CDS             complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="ACFOA3_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6897633.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11897..12676)
                     /locus_tag="ACFOA3_000582"
     CDS             complement(11897..12676)
                     /locus_tag="ACFOA3_000582"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6897634.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQRLEQALKARPMAWVGEIGL
                     DFYDKTQTPQQRERQIQVFSRQLAIAQTLRRRVIIHNLKASNEIAAAVRQTGFSCGGI
                     VHAFSGSIEEARVLVGLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     NTVNTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12791..13780)
                     /locus_tag="ACFOA3_000583"
     CDS             complement(12791..13780)
                     /locus_tag="ACFOA3_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6897635.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIHYPDPQAKIAVRTLIPG
                     FAVQELADGLGRLSAQALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13847..14530)
                     /locus_tag="ACFOA3_000584"
     CDS             complement(13847..14530)
                     /locus_tag="ACFOA3_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6897636.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14718..16052)
                     /gene="glmM"
                     /locus_tag="ACFOA3_000585"
     CDS             complement(14718..16052)
                     /gene="glmM"
                     /locus_tag="ACFOA3_000585"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6897637.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRINGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGSL"
     gene            complement(16182..17033)
                     /gene="folP"
                     /locus_tag="ACFOA3_000586"
     CDS             complement(16182..17033)
                     /gene="folP"
                     /locus_tag="ACFOA3_000586"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6897638.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMVGELTGETDAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17173..18066)
                     /locus_tag="ACFOA3_000587"
     CDS             complement(17173..18066)
                     /locus_tag="ACFOA3_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6897639.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18157..20271
                     /locus_tag="ACFOA3_000588"
     CDS             18157..20271
                     /locus_tag="ACFOA3_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6897640.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20336..21814)
                     /gene="ubiD"
                     /locus_tag="ACFOA3_000589"
     CDS             complement(20336..21814)
                     /gene="ubiD"
                     /locus_tag="ACFOA3_000589"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6897641.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMVPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFHKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPMFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21834..22349)
                     /locus_tag="ACFOA3_000590"
     CDS             complement(21834..22349)
                     /locus_tag="ACFOA3_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6897642.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSELDCSG"
     gene            complement(22356..22790)
                     /locus_tag="ACFOA3_000591"
     CDS             complement(22356..22790)
                     /locus_tag="ACFOA3_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897643.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIINGTL
                     TALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23071..23154)
                     /locus_tag="ACFOA3_000592"
                     /pseudo
     CDS             complement(23071..23154)
                     /locus_tag="ACFOA3_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23157..23657)
                     /locus_tag="ACFOA3_000593"
                     /pseudo
     CDS             complement(23157..23657)
                     /locus_tag="ACFOA3_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5340 a         6764 c         6211 g         5357 t
ORIGIN      
        1 tcctgatttt tgttaattca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttaaaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggcc
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gaggccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttaa tttcaagact tccgcaaatg
     1381 cgcgggcaac atagtcggta ttcgacgtat tcagtccggc gacgcacatc ctgccggaat
     1441 ccagcaggta aacggcaaat tcgtcgcgca gcctgcggac ttgttccacg ctcaatcctg
     1501 tgtagccgaa catgccgcgc tgtttgatga aataagtgaa atcgcgatcg gggatttgcg
     1561 cagttaagac atcataaagt ttctgccgca tcgcacggat gcggtcgcgc atcatataaa
     1621 cctcgttttg ccacaaggcg taaagttcgg ggctgttcat cacgtcggcg gcgatatacg
     1681 cgccgtgcgc gggcgggctg gagtagatgc ggcggacggt gaatttgagc tgtccgaaca
     1741 ccaaatccgc ttcttcctta ttcgggcaaa ccacgcttaa gccgccgacg cgctcgccgt
     1801 agagcgacag gttttttgag aaggaattgc tgacgaacaa gggcaattcc atttccaccg
     1861 ctttgcggac ggcgtaggca tcgctgtcca aatcgccgcc gaagccttgg taggcaatgt
     1921 ccataaacgg aatcagtttg cgcgttttga tgatgtgcaa cacttcgtcc cattgttgtt
     1981 ccgacatatc cacgccggtc gggttgtggc agcagggatg gaggatcagg acgctgtttt
     2041 cgggcagggt gttgaaaaac gcggtcattt cgtcgaattt cacgccaaca gtggcagggt
     2101 cgtaatatgg gtaagtgccg acctcgaaac ctgcgccttc aaaaatgccg cgatggttgt
     2161 cccaagtcgg gtcgctgacg taggcgcgcg cttcgggaaa ccagcggtgc aggaagtccg
     2221 ccccgacttt gagtgcgccc gagccgccca aggtttgtac ggtaacgatg cgcccttgcg
     2281 caagcgcggg attgtctttg ccgaacaaca aatgctgcac cgcgctgcgg taagtgtcca
     2341 agccttccat cggcaggtag ggcgacggcg caggcgcggc ggcacgggca gtttcggcgc
     2401 ggctcacgga ttccaacacg ggcattttgc cttcgtcgtc gaaataaatg cctatgctca
     2461 aattgacttt ttcggggcgc gggtcgtttt tgaaggtttc gaccaaactc aaaatcgggt
     2521 cgccgggata gtattcgatg tgtcggtaca tagtccttac cttttacttt ttcaaaggat
     2581 tttctttttc aacaatacac cactttcgat atggtgcgta aacgggaatt ggtcgaacag
     2641 ggcggcgcgt tcgaccgcat gggtttccac caaggtgtcc aaattggcac gcagcgtttc
     2701 gggattgcag gaaatgtaga tgatgttgtc aaactgcgac accagcttca aagtttcctc
     2761 atcgataccg gcacgcggcg gatcgacgaa aatagtggaa aatgcgtaat ccgtcaaagc
     2821 aataccgcca tccttaaggc gtttaaactc acgttttccg gtataggctt cggtaaattc
     2881 ttcagcagac agacgggcga ttttgatgtt gccgatgcgg ttggcttcga tattccattg
     2941 cgccgcgctg atggaggttt tggagatttc ggttgccaaa acacgttcaa acttttcaga
     3001 taaaggaagc gtaaagttgc cgtttccgca atacagttcg agcaaatccc catccaatcc
     3061 ttctgctgca ccgcaggccc attcaagcat tttctgacac acggcggcat tcggttgggt
     3121 aaaactgcct tcaatttgcc gataacggaa atcccggttg ccgaccttca aagtttccgt
     3181 tacatagtcc tgttttaaga ctattttctg tcccctgctc cgcccaataa cggaaatatc
     3241 caactgttgc tgtaacgctt gcgccgcctg catccactca tcatcaagcc ttttgtggta
     3301 aatcatggta accagcattt ccccgctgag cgtagacaaa aattcgacgg cataccaacg
     3361 gtttctcagt tcggcagatt gggcggcaac ggcaattaat tcgggcatca ggcagttgac
     3421 tgcctctgaa gcagcatcga agcggtcgca acggatcagg cttgcgccgc tggctttctg
     3481 ccctttttca aacatggcat aaaacatttc cccgccttcg tgccaaatac ggaactcggc
     3541 acgcatacgg tagtgtttgt ccggagattc gtacacttcc cactcaggaa catccaaacc
     3601 tgcaaaaagg gttttaaggt ggtctttttt atcttgaagc tgctgcgtgt agtcgctcat
     3661 atcgctatcc tgaaaaatca gaccggatta taagccgatt cactgcaaaa cacgcaaccc
     3721 ctttaccggc atataacccg ttttcctcca acttttatgc taaaatccgc cgtctccaac
     3781 ataccgccga cagaaggaaa atatggcagg caacactttc ggacaactct tcaccgtgac
     3841 cacgttcggc gaaagccacg gcgcaggttt gggctgcatc atcgacggct gcccgcccgg
     3901 cttggaatta agcgaagcgg atatccaatt tgacctcgac cggcgcaaac ccggcaccag
     3961 ccgccacgtt acccaacgcc gcgaagccga ccaagtcgaa atcctctccg gcgtattcga
     4021 aggcaaaacc accggcacac ccatcgccct cttaatccgc aataccgacc agcgcagcaa
     4081 agactatggc aacatcgcca ccagcttccg ccccggccac gccgactata cctattggca
     4141 caaatacggc acgcgcgact accggggcgg cggcaggagt tccgcccgcg aaaccgccgc
     4201 ccgcgttgct gccggagccg ttgccaaaaa atggttgaaa gaaaaattcg gcacggaaat
     4261 caccgcctac gttacccaag tcggcgaaaa agaaatccgg tttgaaggct gcgaacacat
     4321 ttcccaaaat cctttttttg ccgccaacca tagccaaatt gccgagctgg aaaactatat
     4381 ggacggcgtg cgcaaatcct tggattccgt cggcgcgaag ctgcatattg aagccgccaa
     4441 tgtccctgtc ggcttgggcg aacctgtttt tgaccgcctc gatgccgaaa tcgcctacgc
     4501 gatgatgggc atcaacgccg tcaaaggcgt ggaaatcggc gcaggttttg acagcgtaac
     4561 gcaacgcggc agcgaacacg gcgacgaact gaccccgcaa ggcttcctgt ccaaccattc
     4621 gggcggcatc ctcggcggca tcagcaccgg gcaagacatc tgcgtcaata tcgccatcaa
     4681 acccaccagc tccatcgcca ccccgcgccg cagtatcgac atcaacggca accccatcga
     4741 actcgccacg cacggcaggc acgacccctg cgtcggcttg cgcgccgcgc cgattgccga
     4801 agctatgctc gccttggttt taatcgacca cgccctgcgc catcgtgcgc aaaatgcgga
     4861 tgttgccgtg gatacgcccg atatcgcccg ctcggacaag taaaaagatt tagccaaaac
     4921 acgggcttta tactaaaata ccgaccattg ccgtgccgtc tgaacgcacg attcttagag
     4981 gaatacaacc gaaatgacac aagaaaccgc tttgggtgcg gcactgaaat ccgccgtcca
     5041 aactatgagc aaaaagaaac agaccgaaat gatcgccgac cacatctacg gcaaatacga
     5101 tgtgttcaaa cgcttcaaac cgttggcact cggcatcgat caggatttga ttgccgcttt
     5161 gccgcaatac gatgccgcac tgattgcacg cgtcctcgcc aaccactgcc gccgtccgcg
     5221 ctatctgaaa gccttggcgc gcggcggcaa acgtttcgat ttgaacaacc gtttcaaagg
     5281 cgaagttacc cccgaagaac aggcgatcgc gcaaaaccat cctttcgtgc agcaggcttt
     5341 gcaacagcag tccgcccaag ctgccgccga aacgccgtct gttgaagccg aagcagcaga
     5401 atcttccgca gcagaataaa tccccaaatg aaatgccgtc tgaaaaccga tttggtttca
     5461 gacggcattt ttccgtatgc ggcaatcacg gttcaaatat cgagttccgc cgtatcgcct
     5521 tcgcgctcca tccaggcacg gcgggcggcg gcttcgcctt tgcccatcag tttgacgaag
     5581 atgtcgcgcg tctcgtcatc cgcgccttcg gggatttgta cctgcaacag gcggcgggtg
     5641 tcggggtgca tggtagtatc tttgagctgg tctgggttca tctcacccaa acctttgaaa
     5701 cggctgatgg aataagcagt ttctttaacg ccttcttttt gcagccgctc taaaatgccg
     5761 tcaagttcgt tttggtcgag ggcgtagaat ttgcgggcgg atttgctttt accttgtgcg
     5821 ttgacatcta cgcggaacag cggcggctgg gcgacgtaga tgtgtccgtc agcgaccagt
     5881 ttcgggaagt ggcggtagaa cagggtcagc agcaaaactt gaatatgcga gccgtccacg
     5941 tcggcatcgg acaggatggc gattttgccg taacgcaggc cgcttaaatc gggatggtcg
     6001 ttgatgccgt gcggatcgac gccgatggca actgaaatgt cgtggatttc ggcgttgccg
     6061 aagagctggt cggggtggac ttcaaagctg ttgagcactt tgccgcgcag gggcaggatg
     6121 gcctgggtgg ctttgtcgcg ggcgagtttg gctgagccgc cggcggaatc gccttcgaca
     6181 aggaagagtt cgttttcgcg gatgtcttcg ctttcgcagt cggtcagctt gccgggcagg
     6241 acggcgacgc cgctgccttt tttcttttcg attttcttaa ccgaacgcat ccgcgcctgc
     6301 gcctgacgga tggcgagttc ggcaattttt ttaccgaagt ccacgttttg gttcagccac
     6361 aattccaaag ggtcgcccgc tacagcggcg acaagtttca gcgcgtcgcg gttggtcagc
     6421 ttgtctttgg tctgaccttg gaactgcggg tcgaggacac gggcagagag gacaaaggcg
     6481 gttttgccga acacgtcgtc gctttgtact ttcacgccgc gcggcaagag gttgtgcaga
     6541 ttgatgaaat tgttgacggc gttgaacacg gcttgtttca agcctgcttc atgcgtgccg
     6601 cccaaagggg tgggaatcag gttgacgtag ctttcgttgg cacacgagcc ttcttccagc
     6661 caagtcaggg caaacgccgc tccttcgccg atgctgaaat cgccgttgtg ttcgtctgaa
     6721 atgtagtttt cgcaagagaa gataggcacg gcttcctgcg cgtcggcaat caggtcggtc
     6781 agatagcttt tcaggccgtc ggggtagtgc caagttcggg tatgcgcttc gtcttcgcct
     6841 ttgaccggac gggtcaggga aacgcgcaca cccggcagca gcacggcttt ggcacgcagc
     6901 aggcgttcga gttcgggaat gctgtaattc gggctttcaa aatatttgcc gtccggccac
     6961 acgcgcactt cggtgccgct gtctttgacg gcgcatttgc ccacttgtgc caacggttcg
     7021 accacgtcgc cgccggcaaa cacgatacgg tggattttgc cttcgcgttt gaccgttacc
     7081 tcaaggcggg tggaaagggc gttggtcacg gatacgccca cgccgtgcag gccgcctgaa
     7141 aaggcatacg cgctgccgcc gtctttttta ttgaacttgc cgcctgcgtg cagacgagtg
     7201 aacacgagtt cgaccacggg tacgccttct tcagggtgca ggccgacggg aataccgcgg
     7261 ccgttgtcgc gtacggaaag cgaaccgtct tcatgaattt gcacgtcgat tgccgtagcg
     7321 aaaccgccca acgcctcgtc cgccgcgttg tcgatgactt cttggcagat atgggtcggg
     7381 ctgtcggtgc gggtgtacat accgggacgt tctttgaccg gctccaagcc tttgaggacg
     7441 gtgatgctgg attcgctgta ttggttgttt ttagccatag gaataatctg aaagtaagaa
     7501 aaacaacgct ttcagacggc ctgaaagcgt tgcgttccgt tgttttagcg gttgtcggaa
     7561 gattggcggg cggcaaagtc ttcataactt tccataccgc gcaggaagcg ggaagagagt
     7621 tctttcaacg cccccaaata aacgtcgcgt ttgaaatcaa tcacttcgtc aacgggcgcc
     7681 caatattgat gccaacgcca gccgtcaaat tcggggtggc gggtggcgcg caggttgaca
     7741 tcgcaatctc ggccggtcag gcgcaggaga taccaaatct gcttctgtcc gcgataagag
     7801 ccgcgccatt cgcggcgtac ccagttgttc ggcacgtcat aacgcagcca gtcgcgcgtg
     7861 cggccgataa ttttgacgtg ttgcggcaaa agcccgactt cttcgtacaa ctcgcggtac
     7921 atggcggttt cggggctttc gcccggcttg atgccgcctt gcggaaactg ccaagaatgt
     7981 tcgcgcacgc gcttacccca aaagacttcg ttgcggttgt tgattaagat gataccgaca
     8041 ttggggcgat agccttccct gtccaacacg gtgtcgccct ccgttaaatt caatcttggg
     8101 attttcccac aaatcaggcg gttttgacaa atcagacggc atggcggtac gcgtgccgaa
     8161 acacgggggg atttgggaaa atatcttaaa tttggtttac aataatgtat ttcaaattat
     8221 tcgggaatca gaccatgtta gatatccaat tgctccgcag caacaccgcc gccgttgccg
     8281 aacggcttgc acggcgcggt tatgactttg ataccgcacg ttttgacaca ctggaagaac
     8341 gacgcaagtc cgttcaggtg aaaaccgaag aattacaggc ctcgcgcaac agcatttcca
     8401 aacaaatcgg cgcactgaaa ggtcagggca aacacgaaga agcgcaggcg gccatgaatc
     8461 aggttgccca aatcaaaacc gatttggaac aggctgccgc cgatttggat gccgttcaaa
     8521 aagaattgga cgcatggttg ttgagcatac ccaacctgcc acatgaggac gtgcctttcg
     8581 gtaaggatga aaccgaaaac gtcgaagtcc gcaaagtcgg cacgccacgc gaatttgact
     8641 ttgaaatcaa agaccatgtc gatttgggcg aacctttggg tttggatttc gaaggtggcg
     8701 cgaaactttc cggcgcacga tttaccgtga tgcgcggaca aatcgcccgc ctgcaccgcg
     8761 cgctggcgca attcatgctc gatacgcaca cgctgaaaca cggctacacc gagcattaca
     8821 cgccttatat cgttgacgat acgacgctgc aaggcacggg tcagctgccg aagtttgcag
     8881 aagatttgtt ccacgttacc cgtggcggcg acgagagcaa aaccacgcaa tatctgattc
     8941 cgacagctga agttaccctg accaataccg ttgccggcag cattatcccg tccgaacaac
     9001 tgccgctgaa gctgaccgcg cattcgccct gtttccgcag cgaggcgggt tcgtacggca
     9061 aagacacgcg cggtctgatt cgccagcacc agttcgacaa agtggaaatg gtgcaaatcg
     9121 ttcatcctga aaaatcatac gaagcgctgg aagaaatggt cggccatgcc gaaaacatcc
     9181 tgaaggcttt ggaactgccc taccgcgtga ttaccctgtg taccggcgac atgggcttcg
     9241 gcgcaaccaa aacgtatgat ttggaagttt gggtaccggc gcaaaatacc taccgcgaaa
     9301 tctcaagctg ttccaactgc gaagatttcc aagcccgccg cctgaaggcg cgtttcaaag
     9361 acgaaaacgg caaaaaccgc ttggtacata ctttgaacgg ctccggcttg gctgtcggca
     9421 gaacgctggt cgccgtattg gaaaaccatc aaaacgccga cggcagcatc aacatccctg
     9481 ccgcactgca accgtatatg ggcggtgttg ccaagttgga agtcaaataa gtttgcaggc
     9541 tgcctgaacg tcaaatgccg tctgaaacct gtttcagacg gcatttcctt taaactttta
     9601 aaacacgtca gccgtcggca cgaaccgcat tgccgcaatc gccggtctgt ccgacctcgc
     9661 ggatattgga cagcgtaact tccgaaatat tacccaacgc ctcttccgtc aaaaatgcct
     9721 gatggccggt aaacagcaca ttatgacaag acgacaggcg gcggaacacg tcgtcggtaa
     9781 tcacatcgtt ggatttgtct tcaaaaaaca gctcgcgctc gttctcgtac acatccatgc
     9841 ccaatgcgcc gattttccgg cgtttcaacg cctcaatcgc ggcggcactg tcaatcagcc
     9901 cgccccggct ggtgttgata atcatcacgc cgtctttcat tttgtcgaac gccgcttcgt
     9961 tcagcatata gtggttttcc ggcgtggcgg ggcaatgcag cgtgatgatg tccgaccggg
    10021 catacagctc gtctaaatcc acatatttgc cgccgatttt ttccgcttcg gggttgcaaa
    10081 acggatcgta agccagcagg ttcatgccga aaccctttaa aatccgcatg gttgcaatac
    10141 cgattttccc cgtgccgata acgcccgccg ttttgccgta catattgaaa ccggtcagac
    10201 cttccagcga aaaattcgca tcgcgggtac gctgataggc tttgtggata cggcggttca
    10261 acgtcagcat cagaccgacc gtatgttccg caaccgattc gggcgaatag gcaggcacgc
    10321 gcacaacttt caagcccaac tcttcagccg cctttaaatc cacattattg aagccggcac
    10381 aacgcaacgc cacagttttc acgccaattt gcgccaattt ttccaacacg ggccggctgc
    10441 cgtcgtcgtt tacaaaaata cagaccgcct ccgcaccttc cgccattttc gccgttttca
    10501 catccagcat aaaatcaaaa aactccagct cgaagccgaa atgccggttg gcgcgggtaa
    10561 aatgttcgcg gtcataactt ttcgtaccgt aaatcgcaat cttcatcaat atgtccagtt
    10621 gtcgcctatg gttgagaaac ggcataatac actgaattca aacaaatcag tagaatatgg
    10681 cagattaaaa ttgattgcat gcacggcatt tccatttcaa aacacaaaac tcaatcgccc
    10741 attgccgcca gaagctccgc ctgatgctcg gcaatcaggg catcggtgat ttctgccaaa
    10801 tcgccgtcca tcacaaaatc cagcttgtgc agggtgaggt tgatgcggtg gtctgttacc
    10861 ctgccttggg gatagttgta ggtgcggatg cgttcgctgc ggtcgccgct gccgatgagg
    10921 gatttgcgtt cggcggcttc tttggcttgg gcttcgcgtt tttgcgcgtc gttcaggcgg
    10981 gcggcgagga ctttcattgc ctgcgctttg ttggcgtgtt ggctgcggcc gtcttggcat
    11041 tcgaccacca taccggtggg caggtgggtg atgcggacgg cggaatcagt tttgttgatg
    11101 tgctgaccgc ccgcaccgga tgcgcggaag gtgtcgatgc gcagatcggc ggggttcaac
    11161 tcgatgtctt ccagttcgtc cgcttcgggc atgacggcaa cggtgcaggc ggaggtgtgg
    11221 atgcggcctt ggctttcggt ggcggggacg cgctgcacgc ggtgtccgcc cgattcaaat
    11281 ttcaaacggc tgtacgcgcc taagccgata atacgggcga taacttcttt atagccgccc
    11341 aactcgcttt cgttggcgga cacgatttca acctgccagc ggttgcgttc ggcgtagcgg
    11401 ctgtacatac gcagcaaatc gccggcaaac agcgcggctt cgtcgccgcc ggtaccggcg
    11461 cgtacttcga taaagatgtt tttgtcgtcg tcggcatctt tgggcagcag caatttttgc
    11521 agttcggtat cgagcgcacc gattttggct ttcgccgctt cgatttcttc ggcggcaaag
    11581 tctttcattt cggggtcgga caacatttct tcggcatccg ccaagtcgct ttgggcaagc
    11641 cgatagtttt ggaacacttc gacgacgggg gtcagttcag cgtgttcgcg cgtgagcttg
    11701 cggtagttgt ccatatcgga cgtggcttcg ggttgcccta aaaggtgggt aacttcttcc
    11761 aaccggtcgc cgagttgttg cagtttttct aagatagacg gcttcataat tcttccataa
    11821 caaacgccgc ctgaagttca gacggcatta aaacgcggtc attataatat attttctgaa
    11881 tattcaaaaa gataatctag ggacggagca ggctgtcggc attgcgttcc gttatcgcgg
    11941 cgatttccgc cgcgccggta ccgcgtatct gcgcggcaat ttcggcaatc cgaagaatat
    12001 tggcaggcgt attgacggtg tttttcaaca tgaacgggct atccgtttcc aatacgaaat
    12061 ccccgtcgtt caaggcttta agcgtatcgc gcactttacg cgcgttcggg ttgagcagca
    12121 gcgaaccgat accgattttg aaaccgagcc cgaccaacac ccgcgcttct tcaatgctgc
    12181 cggaaaaggc gtggacgatg ccgccacagg aaaaacctgt ctgtctgacg gcggcggcaa
    12241 tctcgttcga ggctttcagg ttatggataa tcacgcggcg gcgcagggtt tgcgcgatgg
    12301 caagctggcg gctgaaaact tggatttgcc gttcgcgctg ctgcggcgtt tgggttttat
    12361 cgtaaaaatc caagccgatt tcgccgaccc atgccatcgg acgcgctttc aatgcttgtt
    12421 ccaaacgctg gaaatccggt tcggaaacgt cgtctgaaaa ccaaggatgg atgccgagcg
    12481 cgatatggat attgccccaa agcggatttt cagacaacct ttgagccaaa tccgccacgt
    12541 cctgccaatc ctgcggccga gtcgcgggaa cgataaaccg ccctacccct gcctcccgcg
    12601 cggcggtcag gatgtgcggc aggttttcgc gcagggcggc atcggcaagg tggcagtggg
    12661 tgtcggtgaa gttcatttcg attccgcaat gcggcaggtt cacataaaaa taaggcaggg
    12721 cggcaaggga tgccgtgtac gggcggcgca taaggcgcgg tcaacgcccc gtcattgcca
    12781 tttcaatccg ctataacttc agtcttgcca actctttttc cacctcttcc ttaccccagc
    12841 cttgcgatac ggcgagggtc atgagtgcgg tggcggtttc gaggttgcat ttgccgccgt
    12901 tgatgatgcc cgagttgcgg aacgcattgc cttgcgcgta aacggcggcg gttttgcctt
    12961 gtcggacttg gctgatgttg agcaacaatt ttccttgccg cgtgaagtct cggacagcgc
    13021 ggataaagcc ttcgtctgcg ggggtgttgc cgtgtccgta gctttgcagg ataagggctt
    13081 gggcggaaag ccgtccgagt ccgtccgcaa gttcttggac ggcaaagcca gggataagcg
    13141 tgcggacggc gattttcgcc tgcgggtcgg gataatggat tttgaggccg tctgaaacat
    13201 ctgccgcgtc ctgattcggg atgcggacat tgtgccagcc ctgggtttcg tcccactccg
    13261 ccagtgcgcc gaaatgcgga ttgtcgaaac cggcggcggt ttcggtgctg actttgctgc
    13321 tgccgacggc gggatacagt ttgccgtcaa aggcaataag cgtctgcttc aatccgagtg
    13381 ggaatgcggc aacggcagtc gccagattgc gcggcgcgtc gctgcccacg gcgttgtacg
    13441 gccattggga gccggtcagg acgattggct tgtccaaacc ctgcagggcg agggcgaaca
    13501 gattggcggt ataggcgagc gtgtccgtgc cgtgcagcac caaaacgccg tcgtactgcg
    13561 gcagcgcggc ggcaatgatg tccagccaag cgcaccagtt ttgcagttgg acggcggaag
    13621 aatcaatcag cggcccacaa acgtgccaat cgaaatccat gccgtttgaa aacggcgcga
    13681 gtgcctgccc gaccagcgcg gtatcggggc gcaggccttc gctgctttgc gtcatgccga
    13741 tggtgccgcc ggtataaagg acaaaaattt tctgtttcat ggctcatcct gaaaatgcaa
    13801 agaccgcctg aaacgcgaaa tgcgtccgga cggtcgaaaa tctgatttac tgctgcttct
    13861 gtgccgcttt tttcgctgcc ttttccgcct tgcgtttggc gcgtttttcg ctcaattgtg
    13921 cgcggtaaag ctgataatgt cggcgtttgc gccaccagaa ccaagccagc gccgccgcca
    13981 gcacgcccaa tgcgatgaag atgccggatt gcaggctgtg cattttcgcc atcagccaat
    14041 cgatgttgtg cgcgccgtac tcgcccaagt aaatccaaac gggcacggaa atcagcgcgg
    14101 caagcccgtc cataatcaga aagcgcagat acgatacttt gcggctgatg ccggcggtaa
    14161 cgaaaacggc agtccgcaaa ccgggcagga aacgagcgac aaataacacc cagttgccgt
    14221 atttgtcgaa tttttcctga acctgtgcgt aacgtttcgg cgtcatgatg cgcgcaatcg
    14281 gtttgaactt gaggattttc tgcccccaaa ttcgtccggc ggcgaacatg atgccgtcgc
    14341 ccgccaacac gccgagcatg ccgactgcaa acataatatg cggattggta taacccatac
    14401 ccgaaatcac gccgcctgtt accaaggtca aatcctcggg aatcggcacg ccgaaaccgc
    14461 agatgaccaa aacgaaaaac acggccgcgt agccgtattc gacaaaaaag gcttccaaaa
    14521 gggcaaacat agcggatatt ccattgtcgg agataaaaag tcagaacaag ccgaaacatt
    14581 ttctgcgcga aacggcattc tatcaaagat tatgccgtct gaaagcggca gggggcagat
    14641 tcgcgccccc gccggacgtt cagacggcat ttaaacagga aaaccgccgc tgttgaaacg
    14701 gttttgcctg attttgccta aaggctgccg atggcggcgg caatgcgttc cgcaccatcg
    14761 cgcgcccaat ccgcctgccg cgcctccacc atcacacgca cgacgggttc ggttcccgaa
    14821 gcgcgcaaca cgacacgccc tttgccttcg agttcttttt ccacttccgc caacacgtct
    14881 tttgaggctt cctgccagtt ttgccctttg ccgatacgga cattaatcat cgtttgcgga
    14941 tacggctgcc aatcggcgca aacggtggcg aggtcttggt tcagcgtttg cagtgccgcc
    15001 aaaacttgca gcgcggaaat aatgccgtcg ccggtgttgt gtttgtccat gcacaaaata
    15061 tggccgctgg cttcgccgcc gatgagccaa ccgcgttggt tcagctgttc caacacatag
    15121 cggtcgccga ctttggcgcg gcagaaatcg acgccctgct ctttcagggc gatttccatc
    15181 gccatattgg tcatgaccgt gccgaccacg ccgccgatgt tgataccttc tcgggcgcgg
    15241 gctttggcaa tgacgtaaat caggctgtcg ccgtcgtaaa ccttaccgtt tttatcgacc
    15301 atcatcaggc ggtcgccgtc gccatctaag gcgataccat agtcggcttc atgctgtaaa
    15361 acggcagcct ggagtgtctt ggtataagtc gcaccgcatt tttcgttgat gttgtagccg
    15421 ttgggttcgt cgccgatgct gacgacttgt gcgcccagtt cgtgaaacac tttgggtgcg
    15481 acgctgtaag ccgcgccgtt ggcggcatcg acgaccagct tcagcccgcg caaatcggaa
    15541 tgcgtaggga aggtggattt gcaaaattcg atatagcggt cgtccgcgcc gttgatgcgg
    15601 cgtgcgcgac cgagacgggc ggacggttgg gttttcattt cgccgtcgat tttggcttcg
    15661 atttccaact cgacttcatc ggaaagtttc acgccgcctt cggcaaagaa tttgatgccg
    15721 ttgtcggaat aggcgttgtg cgacgcggaa atcatcacgc cggcggacag gcgcaacgcg
    15781 cgggtcagat aagccacgcc gggcgtgggc agcggtccgg tctgtaccac attcacaccc
    15841 gccgccgtaa aaccggccac caaagcggct tccagcatat agccggaaat gcgcgtgtct
    15901 ttgccgatga ggacggtcgg tttctggtcg gtgtcgtgct gcaccaaaac ctgccccgcc
    15961 gcatagccga gtttcaatac gaaatcgggc gtaatcggaa attgccccac ttcgccgcgc
    16021 acgccgtccg tgccgaaata tttttttgcc atgtgttgct ccgagaatgt ggaccgttgt
    16081 ccgagattat acagtcagtt tgtgccttgc tgtctgcacc gttgatgccg tctgaaacca
    16141 ccccgccctt ttcagacggc atgaagtatg cgaaccgctg tttacagatt gatgcccaac
    16201 gcttcccaca ccttcaacgc gtccgccgtc gccttcacat catgcacccg cacgatttgt
    16261 gcgccgcgcg ccacggaagc cagcgccgcc gccacgctgc cgtgtacgcg cgccgccgcg
    16321 tcggtttcgc ccgtcagttc gcctaccatg cgtttgcgcg atacgccgat tagcagcgga
    16381 aagcctgttt ccgccatcaa ttcgggcaaa tgccgcatca gcgcgatatt gtgttgcagg
    16441 tttttgccga agccgaaacc cgggtcgagc gtgatgcgtt gcggtgcgat gcctgccgcg
    16501 atgcattccg ccgcccgcgc tttcaaatac cgcgccactt caccgacaac atcctgatat
    16561 ttcggattaa tctgcatgtt ttcaggcaaa ccccgcatgt gcatcaggca aatgcccgtg
    16621 tccgtctggc gcgccagcaa ttcgactgcg ccttcgtcgg tcaacgccgc cacatcgttg
    16681 ataatatcga taccgccgag tgccaacgct ttttccataa ccaccgtgcg gcgcgtgtcc
    16741 aaactgacgg gaacacccca ccccgccact tccgccaata caggctcaac ccgcgcccat
    16801 tcttcttcag gcgaaacata atccgcaccc ggccgcgtcg attcgccgcc gatgtcgaga
    16861 atgtccgcgc cctctttcaa aagccgttcg gcgtgcgcca aagcggtttg ggcgttttgc
    16921 gaatacgcgc cgccgtcgga aaaagaatcg ggcgtgagat tcacgatacc catgattttc
    16981 ggtttgtcca aaccgatttc aaaccgtcct gcctgccaaa cgtgtcgtgc catctgaact
    17041 cctcccaaaa taaaaaacag attatatgcc gtctgaaacc gtcttgtgcg cttcagacgg
    17101 caccgctatt cgggcggcag acggcatgtt gtccgaatgt ctgctccgcc tttgaatctg
    17161 ccggtatgcc tgctatccgc ccgacttttc aaaacaggtt ccgacgattc cgcacgcgcc
    17221 tgccgccttt gccaagccgt acaggatttc ctgcggcata tcgcggttcc ataatcccgt
    17281 aatattcgca atcacgggca gatggctgat ttggcggact ttcacgatgg attcgacatc
    17341 caaacggtag ggatggtctt tggtatggct caacacgccc gactcgccta gaatcaggtg
    17401 gcggttgccg cgcgagacga catattctgc ggcattcaac caatcttcgg cagaatggtg
    17461 cttgtcttta cacagcacca agggaatgtt caggtttccg gcttcattca atacggcaag
    17521 atcggacatc agcccgccgc ccaaatacag gatgtccgcc cccgcattca aagccgcttc
    17581 gacatggcgg acgttgcgga cgcgcaccaa tacgggtttc cctgcgccat gcgccgatgc
    17641 ggtctgttcc gccaaccgtc tgcaccgtcc ccgcccttca tccgcacttg aagtgtcgta
    17701 taagtttgcc gaagtgaaaa acggatccag aaacactgca tccgcattgc gccatactga
    17761 cggttctgcg gcgatacgga cggtttcccc gccgccgaaa gccacgcctt tggcggcaac
    17821 gcggctgtct tccgcccgat tttcccgact gacggttttc catgtatcca aaatgcggac
    17881 ggctttctcg acctccggca gcgtctgcac ctccctgacg ctcaaaaccc tatcgtcgcc
    17941 gattgcgccg atgacagtac gctcgtcgcc gtgagaaatg tgttctcgca gacctctgct
    18001 gcggataaag gcgacaacgc cggcaatgtc cgcttcggcg gcacgcctgc tcatgacaat
    18061 aatcatattt cctcctgaca caagaaacgg cctgcccaaa ataggatttt tgcaagccgt
    18121 gttatactgt ggcgtgtttt acagattgtt cgggctatgg atttattatc ggttttccac
    18181 aaataccgtc tgaaatatgc ggtggccgtg ctgacgatac tgcttttggc ggcagtcggg
    18241 ctgcacgctt ccgtatatcg caccttcacg cctgaaaaca tccgcagccg cctccaacaa
    18301 agcattgcac acacacaccg gaaaatctcg tttgatgcgg acattcagcg caggctcctg
    18361 ccccggccga ccgtcatcct gaaaaacctg accattaccg aacccggcgg cgaccggact
    18421 gccgtttccg tccaagaaac caaaatcgga ttgagctgga aaaacctgtg gtcggatcag
    18481 atacagattg aaaaatgggt ggtttcgagt gcggaacttg ccctgacgcg cgacgggaaa
    18541 ggtgtttgga acattcaaga cctgatcgac agccaaaaac gccaagcctc agtcaaccgc
    18601 attatcgtcg aaaacagcac cgtccgcctc aatttcctgc aggaacagct tatcctgaag
    18661 gaaatcaacc tcaacctgca atcccccgat tcgtcggggc agccgtttga aagttcgggc
    18721 atactggttt ggggaaagct gtccgtcccg tggaaaagca gggggctgtt cctttcaaac
    18781 ggcatcggcc cgcccgaaat ctcaccgttc cattttgaag cttccacttc gctggacgga
    18841 cacggcatta ccatttccac caccggcagc ccttctgtcc gcttcaacgc cggcggagcg
    18901 gatgccgccg gcctcggcct gcgtgcagac acttccttcc gtaacctcca cctgaccgcc
    18961 caaatcccta cgctggcact caggaacaac agcattaaaa ttgaaaccgt caacggcgca
    19021 tttaccgccg gcggcgaata tgcccaatgg gacggttcgt tcaaactcga caaagccaac
    19081 ctgcactccg gcatcgccaa catcggcaac gccgaaatct ccggcagctt caaaacaccg
    19141 cgccaccaga ccaacttctc cctcaattcg ccgctcgtat ggacggaaaa caaagggctg
    19201 gacgcgccgc gcctgtatgt atcgaccctt caggataccg tcaaccgcct gccgcaaccc
    19261 cgtttcatca gccggctcga cggttcgctg tccgtaccga atctgcaaaa ttggaatgcc
    19321 gaattaaacg gcacattcga ccgccaaacc gttgccgcga aattcagata cacacatgaa
    19381 gacgcaccgc atctggaagc cgccgtcgca ctgcaaaaat tgaacctgac cccctatctt
    19441 gacgacgtgc ggcaacaaaa cggcaaaata tttcccgaca ccctcgccaa gctgtccggc
    19501 gacatcgagg cgcacctgaa aatcggaaaa gtccaacttc ccggcctgca actggacgat
    19561 atggaaacct acctccacgc cgacaaagac catatcgcgc tcagccgttt caagtcaggg
    19621 ctttacggcg gccataccga aggcggcatc agcatcgcca acacccgtcc cgccacttac
    19681 cgcctgcaac agaatgcaag caacatccaa atccaaccgc tgctgcaaga cctgttcggc
    19741 ttccacagct tcagcggcaa cggcgacgcg gtcatcgacc tgaccgcggg cggcgaaacc
    19801 cgaaaagagc ttatccgctc gcttcaaggc agcctgtcgc taaatatttc caacggtgcg
    19861 tggcacggta tcgacatgga caatatcctg aaaaacggca tttcgggcaa aactgccgac
    19921 aatgccgcac ccagcacacc cttccaccga ttcacgctca acagcgaaat ttcagacggc
    19981 atcagccgcc acatcgatac cgaactcttc tccgacagcc tctatgttac cagcaacggc
    20041 tataccaatc tggatacgca ggaattgtct gaagatgtcc ttatccgcaa cgccgtccat
    20101 ccgaaaaaca aaccgattcc cctgaaaatc accggtacgg tggacaagcc gtccattacc
    20161 gtcgattacg gcaggctgac cggcggcatc aattcgcgca aagagaaaca gaaaatcctc
    20221 gaagacaccc tgctggaaca atggcagtgg ctcaaaccta aagaaccgta aacatcctgc
    20281 gtacacaaat gccgtctgaa acacccccac gcttcagacg gcatgaccgt aaaacctaca
    20341 accccaattc ctcccaaatc ccatcaatct tagccgtaac cgcagggtct tttttgatga
    20401 cgcgtcccca ttcgcggtcg gtttctcccg gccatttgtt ggtcgcatcc aaacccattt
    20461 tgccgccgag tccgctgacg gggctggcga agtcgagata atcgatgggc gtgttttcta
    20521 ccaaaacagt gtcgcgcacg gggtccatgc gcgtggtgac cgcccagatg acttctttcc
    20581 agtcgcgcac gttcacatcg tcatccacca cgatgatgaa tttggtatac ataaactggc
    20641 gcaggaacga ccagcagccc atcatcacgc gcttggcgtg tccggcgtac tgttttttca
    20701 tgctcaccac cgccatgcgg taggagcagc cttcgggcgg caggtagaaa tcggtgattt
    20761 cgggaaactg cttttgcaag agcggtacga acacttcgtt caatgccacg cccaaaacgg
    20821 caggttcatc gggcggtttg cctgtgtagg tcgaatggta aatcgggttt tcgcgcatgg
    20881 tgatgcgttc gaccgtaaac atggggaaat ggtcctgctc gttgtaatag ccggtgtggt
    20941 cgccgtatgg gccttccagc gcggtttcgt tcggatgaat cacgccttcc aacacaattt
    21001 cggcgcgtgc aggcacttgc aaatcgttgc cgatacattt caccagctcc gtccgcgaac
    21061 cgcgcagcag tccggcaaac tggtattcgc tcaaggtatc gggaacaggc gttaccgcgc
    21121 ccaaaatggt ggcggggtcg cagccgagta cgacggcgac gggatacggc gtatcgggat
    21181 tgagtttgtg gaattcctga taatccaacg cgccgccgcg atgcgacagc cagcgcataa
    21241 tcagcttgtt tttgccgatg agttgttggc ggtaaatgcc gagattttgg cgttttttgt
    21301 gcggcccgcg cgtgacggtc aagccccatg ttaccagcgg cgcaacgtct tccggccagc
    21361 aatgctgaat cggaagttga tacaaatcaa cgtcttcgcc ttcccacacg atttcctgac
    21421 acggcgcgtt tttcaccacg ttcggcacca tgctccaaat gtctttcaag agcggcagtt
    21481 tggaaaacgc gtctttgatg cctttgggcg gttcgggttc tttcaaatac gccagcgtct
    21541 gcccgatttc gcgcagcttg gacacgctgt ccgcacccat gcccattgcc acacgttcgg
    21601 gcgtgccgaa caggtttgcc aacacgggat aatcgtagcg cgtaccgtcg ggcttaactg
    21661 ggttttcaaa caacaatgcc ggcccttcgg cgcgcagcac gcggtcggcg atttcagtca
    21721 tttccaaatg cggggaaaca ggatgcgcga cgcgcttgag tttgccctgc tgctcgagca
    21781 tagcgatgaa gtcgcgcagg tctttgtatt tcatatttat cctttttgtc cttttatcct
    21841 gaacaatcca attcggatac cgcccctatc cttgcctgcg cttcggcata ttctatgccg
    21901 tgataaaagt cgcgtaccag cggatgttcg ccgccttgat ggagttgcaa caaaggacgt
    21961 tgaccatcgg gttgggtaac gacattgcag tgcagaccga aggtgtcggt ttcataaggg
    22021 ggcagctggt tgcagatcat gccgaaataa acagcgtttt caaggttgtc gtaaaagcgg
    22081 ctttgatagt cgttaaaact cttttcgctg acggataccc acacgccata ttccagcgtt
    22141 tcctcatgcc cgataatcgg aatcggcagc accgcgcgga taaagcggtc ggtttggtcg
    22201 ggatagcgga tgatgcagaa atcagaatcg cattctgctt gataagcaat gcgttcttct
    22261 tcactgagtt gattataggg atcgggggcg gtaaagccga ttgcgggcat atcttcatgt
    22321 ttttcgccgc aggaagtgca agtgtacata aggtttcaga ctttcaaaac gagtttgcgg
    22381 taaagccatt cgccggcaaa cagcatcccc atcaatacat aggcaatcac gcctgtataa
    22441 accgcccacc aatcatatcg ccccaaccga gccaacaaag cggtaagcgt cccgttgatt
    22501 ataaaaaata cgcaccaaac ctgcgttacc cggcgggtat agcgcacggc tttttcaggc
    22561 aggtcgggct gttgcagccg cgcaagtttt tcaatcaccg tctgtccggc aaacaggctt
    22621 cccccgaaca ccgccaacat catcagattg acgaggacgg gataccaata cattgaatca
    22681 tgccgcccga acaccaatac tgcggcaaaa aacagtgtaa taaacaaagc cgcataacgc
    22741 tgttggggca atttggcagt cagggcgcgc agcagccaca acccgcacat cgccgccgcc
    22801 agccagacaa accagcccgc ctccctgccg taatatagtg gattaacaaa aaccagtaca
    22861 gcgttgcctc gccttgccgt actatctgcg gcttcgtcgc cttgtcctga tttttgttaa
    22921 tccactatac cacaaagcgg gataggcaat gcttaatacg gtcagaaaaa tatgtccgaa
    22981 aaaaccgggt ttcattttga atccgcacaa atgttttcag acggcatccg ataaaaacat
    23041 gccgtctgaa aaataattag aaatacccga ttagcccgcc tgaatcttca ataccgcctg
    23101 taccacgtcg ttgacggtgc ggacattgcg gaaatcttca gcctgcagct tgcgggctgc
    23161 cacagccatt cacgattgcg atatacccgg cggcattccc gactgccgtt cagcagaaag
    23221 cgcacccatt ccaaaccgct ccaatatgcc tcgggcagca taccggcttc agacggcata
    23281 tcgtccgaag cagataaagt caggctgtaa cgcgtccctt tggtcagaac catcgccaaa
    23341 gcataagcaa acggcgcgcg cgttgccgat acggcatatc cttccggcag cggatcgtcc
    23401 gccgccaaaa ccaaaaccga cccgcatccc tcttccaaca gtgatgccgc ttccgccaat
    23461 gccgtttcca caccgtcggc acacacggcc aatgccgtct gctcgctcat atcccgcctc
    23521 aatatcgacc attaccccgc cgttgcattg tgcaccgaca aaccgaacga agtcggcgac
    23581 acggtttgcg atttcaacag ttccaaccac aaatcgaaac tgcgcgccat ttccccgtcg
    23641 tgcgaggcat aaactatagt ggattaaatt tt
//
LOCUS       DAXIEB010000017        23477 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000017 DAXIEB010000000
VERSION     DAXIEB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.017"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(92..880)
                     /locus_tag="ACFOA3_000594"
     CDS             complement(92..880)
                     /locus_tag="ACFOA3_000594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6897644.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAESIVPVLQNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYAPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFOA3_000595"
     CDS             complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFOA3_000595"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6897645.1"
                     /translation="MPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1624..2349
                     /locus_tag="ACFOA3_000596"
     CDS             1624..2349
                     /locus_tag="ACFOA3_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6897646.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCAADLAAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRIL
                     PRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKQSDANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="ACFOA3_000597"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="ACFOA3_000597"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6897647.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTAHPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="ACFOA3_000598"
     CDS             2889..3011
                     /locus_tag="ACFOA3_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6897648.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="ACFOA3_000599"
     CDS             3008..3727
                     /locus_tag="ACFOA3_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6897649.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTTSADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3781..4107
                     /locus_tag="ACFOA3_000600"
     CDS             3781..4107
                     /locus_tag="ACFOA3_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6897650.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDAHTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFOA3_000601"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFOA3_000601"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6897651.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="ACFOA3_000602"
     CDS             4773..4910
                     /locus_tag="ACFOA3_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897652.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="ACFOA3_000603"
     CDS             4936..6792
                     /locus_tag="ACFOA3_000603"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6897653.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     TLAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFSYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVLIDGQDISSVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFOA3_000604"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFOA3_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6897654.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7744..7833
                     /locus_tag="ACFOA3_000605"
                     /pseudo
     CDS             7744..7833
                     /locus_tag="ACFOA3_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            7942..8205
                     /locus_tag="ACFOA3_000606"
     CDS             7942..8205
                     /locus_tag="ACFOA3_000606"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6897655.1"
                     /translation="MSKWRGFNRKGNTMKKNAPKSFEEALSRLESLTQAMQGEMPLED
                     ALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8189..9085
                     /locus_tag="ACFOA3_000607"
     CDS             8189..9085
                     /locus_tag="ACFOA3_000607"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6897656.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLDGARAYAYQLTDEAVSLL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9194..9493)
                     /locus_tag="ACFOA3_000608"
     CDS             complement(9194..9493)
                     /locus_tag="ACFOA3_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6897657.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9605..11050)
                     /gene="nuoN"
                     /locus_tag="ACFOA3_000609"
     CDS             complement(9605..11050)
                     /gene="nuoN"
                     /locus_tag="ACFOA3_000609"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6897658.1"
                     /translation="MNWSDLNLMPAMPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDDECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11060..12556)
                     /locus_tag="ACFOA3_000610"
     CDS             complement(11060..12556)
                     /locus_tag="ACFOA3_000610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6897659.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIYGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPQAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12666..13829)
                     /locus_tag="ACFOA3_000611"
     CDS             complement(12666..13829)
                     /locus_tag="ACFOA3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6897660.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            complement(14013..16037)
                     /gene="nuoL"
                     /locus_tag="ACFOA3_000612"
     CDS             complement(14013..16037)
                     /gene="nuoL"
                     /locus_tag="ACFOA3_000612"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6897661.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLFVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPVYVLFENKYYLDALYFNVFAKGTCALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(16086..16427)
                     /locus_tag="ACFOA3_000613"
     CDS             complement(16086..16427)
                     /locus_tag="ACFOA3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897662.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(16551..17126)
                     /gene="fic"
                     /locus_tag="ACFOA3_000614"
     CDS             complement(16551..17126)
                     /gene="fic"
                     /locus_tag="ACFOA3_000614"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6897663.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(17160..17465)
                     /gene="nuoK"
                     /locus_tag="ACFOA3_000615"
     CDS             complement(17160..17465)
                     /gene="nuoK"
                     /locus_tag="ACFOA3_000615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6897664.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            complement(17462..18133)
                     /locus_tag="ACFOA3_000616"
     CDS             complement(17462..18133)
                     /locus_tag="ACFOA3_000616"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6897665.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVIAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(18169..18876)
                     /locus_tag="ACFOA3_000617"
     CDS             complement(18169..18876)
                     /locus_tag="ACFOA3_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897666.1"
                     /translation="MEGLINALKYLAKHEPIDNFEEIRIRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSIGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(18907..19386)
                     /gene="nuoI"
                     /locus_tag="ACFOA3_000618"
     CDS             complement(18907..19386)
                     /gene="nuoI"
                     /locus_tag="ACFOA3_000618"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6897667.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(19467..20543)
                     /gene="nuoH"
                     /locus_tag="ACFOA3_000619"
     CDS             complement(19467..20543)
                     /gene="nuoH"
                     /locus_tag="ACFOA3_000619"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6897668.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(20546..22807)
                     /gene="nuoG"
                     /locus_tag="ACFOA3_000620"
     CDS             complement(20546..22807)
                     /gene="nuoG"
                     /locus_tag="ACFOA3_000620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6897669.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGSKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRVSSTQKDFQTASSRLVRVGGVGIYHTDAIVRRSVPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGAGVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(23089..23400)
                     /locus_tag="ACFOA3_000621"
     CDS             complement(23089..23400)
                     /locus_tag="ACFOA3_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6897670.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
BASE COUNT         5975 a         6613 c         5711 g         5178 t
ORIGIN      
        1 cactatacat cggtatttcc tttatttctg tcggttttca cagacagatg ccgtctgaaa
       61 cctggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttca cggcgtgtcc gtacaccgtt tccagcgtaa
      181 tgcccaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacgc catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac gggggcgtaa aagccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttccg ccaaggtatc tcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtcttc agcgcgcgcg cccaattttg cagcacgggg acgatgcttt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgc tgcaccacgc ccttacccgc aaacctttgc caaaaaccgc
      661 ctttgcgcgc ggctgccgcc gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atagcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcaggcg ttggtcggca tattcggcga
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct tcctttcaaa tgccgatgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggtgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc atgcgtatgc
     1201 aaaagctgta attgtagaaa ctgtttgata tgtttggcat aatccgttcg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcagacgggg catcaggtcg
     1561 tcgaacacgt ggcggttcgc cccccacccg tgtatcaggt aaactttttt ggcagcatca
     1621 ggcatgaatt ttctctcttg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg cgcggcggat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag acacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaat atgatacacg cgctgaagca cttggctgat
     1921 ttgagcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcgc aacgctacgg ctggcggata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattttgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaac aatcggacgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatccctc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg tacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcaccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acgacatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggtaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaaca ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcagc caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaggccg aagctcaggc gcgcggtatg gttcgggcgg tattaaccgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacatggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacaacaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgacg acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgccca taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcacacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaagac cttccgccaa ctgatcaagg tcggcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagatgtca aacgcctctc ctccgccccg ggaatcggca aaaaaaccgc cgaacgcatg
     4561 gttttggaac tgcgcggcaa gctggtcgcg catgcggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcggcacgc tgcttgcgct gggttacagc
     4681 gaacgcgaag caaaagcggc ggtcaaaggc gttccggagg ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgcc tgaaggcggc gcggcgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaata ttttcctggt tcgagtcccg aatcgaccct
     4981 tatcccgaag ccgccccgaa aacgccagaa aaaggcttgt ggcggtttgt ctggagcagc
     5041 atggccggcg tgcggaaatg gatagccacc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggta
     5221 ttttcggttg cgtgggcgtt tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccccatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctcgactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggtcgcc accgccaccg ccatggcgtt gcgcgtcaac
     5881 gggctgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc ccaagccctg
     6001 ccgctgaatg tgccgcaagg cgcaatcaaa ttcgaacacg tcgatttctc ctacgaagcc
     6061 ggcaaaccgc tgctcaacgg ctttaacctg aatatcaaac ccggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttt gattgacggg caggacataa gcagcgtcac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccagagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 tgtccgcacg cccgacggca caacctacga agccagcacc cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgcgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctacaacgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc ggcagcctgc gccccgccct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc gcaaaccggg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcatca ccgacgaact gcccgggtaa cgccttgccg cctgggcgga
     7741 aaaatgccgt ctgaagccgg attcgggttt cagacggcat ccgtttttca aaaatgctac
     7801 aatccgcttt ttactggaac atccgaaact atgttccaac acgcaggacg acacttaaag
     7861 caccgcccta tgtgtcgtcc tgatttggaa gggcttacac ccctcccaaa taaagtctga
     7921 ttctaccgcc ccgaaggaca gatgtccaag tggcggggtt tcaaccgaaa aggaaacacg
     7981 atgaagaaaa acgccccgaa atcgtttgaa gaagccttgt cgcgccttga atcgctgaca
     8041 caagctatgc agggcgaaat gcccttggaa gacgcgcttg ccgcctatca ggaaggcaac
     8101 gagctggtca gatactgcca aaccaagctg gcgcaagtcg aacagaaatt acaggttttg
     8161 gacgcggacg ggccgaagga gttgaacctt gaatccgacg aatgatttga aagcgtggca
     8221 acagagggcg caggcgcaaa cagagctgct gctggaacgc tttttgccgt ctgaaaacga
     8281 aataccgcac acactgcacg aagccatgcg ttatgcggtt ttggacggcg gcaagcgtct
     8341 gcgtccgatg ctggtgctgg cggcttcgga attgggcgga gccgtggcgg atgcggtcgg
     8401 acaggcaatg gcggcaatcg aaatgatcca cgtctattct ttggttcacg acgatatgcc
     8461 ggcgatggac aacgacagcc tgcggcgcgg caaaccgact tgccatgtgc aatacgacga
     8521 agccaccgcg ctactggtcg gcgacgcttt gcaaacccaa gccttcgacg tgttaagccg
     8581 tccgacagaa ctgcccgcag cacgccagtt ggcaatgttg tcggtgttgg cgaaagcggg
     8641 cggcagcgcg ggcatggcgg gcggtcaggc aatcgatttg gcgaatgtcg gcaaacaaat
     8701 ggttcaagcc gatttggaac agatgcacag cctgaaaacg ggtgcgctaa tccgtgcggc
     8761 ggttttattg ggggcgatgt cctgcccgaa tctgtctgaa gacagtctgg cggtattgga
     8821 cgattatgcg gcaaaactgg ggctggcgtt ccaagtcatt gacgatgtgt tggattgtga
     8881 agcggacacg gcgactttgg gcaaaacggc gggcaaagac gcagacaacg acaagccgac
     8941 ttatgtaaaa ctgatgggct tggacggtgc gcgggcatac gcgtaccaac tgaccgacga
     9001 agcagtctcg ctgctcgaac ccttcggcga caaagccctg cgcctgcggc agttggcaga
     9061 atttgcagtc gcccgcaaat attaaaaccg gcgtatatcg ccggctgaaa cgtgccggca
     9121 cagcgatgcc ggcctgcact tgaatgaaac aaatgccgtc tgaaaaggtt tcagacggca
     9181 tttttatgct tgtctattcc ctgttgcgcg tgtgccaaaa ataccgccac acgaaacagg
     9241 gaaacgcaaa aatcaggtac aggcagacga cggtggcgta aaactcccaa ccctgatcgt
     9301 gtaccgctcc cgcacgggat tcgaggatgt aggcaagaac ggcggtcagc gcgaaacctg
     9361 ccgccagctc gatcaagtgg tgcccgaaat gtttgcgctt gagcggtacg atgccgaaca
     9421 gcctggtcgt gaggaagggg gcgttggcaa aaatcaaggc aagcagcaaa aggatgtaca
     9481 tggatgcggt catggcgggc tttctgtttg ggcattgtcg gcggcgatgc cgtctgaact
     9541 gcgttgcgga acggcatatc ttaacaaaaa cggcagcctc tgacacggct gccgttgcgg
     9601 cggcttacag cgtgttttcc aacgccttgg cgcaccagtc gataacggtt tgcggcataa
     9661 tgccccacag caccaacagc agcgcgttga cgctcaaaag gaattttgcg gcattgttgc
     9721 cgccgacggt gggacgggcg cggccggatt cgtcaaaata catgactttg accacgcgca
     9781 ggtagtagaa cgcgccaatc agcgacatca cgacggcaaa tacggacaac caaacatagc
     9841 cttgttttaa gagtgccata atcacgccga atttggcgta aaagcccatc agcggcggaa
     9901 tgcccgccat agagaacata accagcagca tcaaaaaggc aagccatacg cggtgttggt
     9961 tcaaccctgc caaatcgctg atgttttcgc actcgtcgtc cccgtccgac aacaccatca
    10021 acacgccgaa ccctgccgcc gccatcagcg cgtaggtaat ggcgtaatag aggcccgccg
    10081 caaagccgac cgcgcccgcc ataaacgcca acaggatgaa acccatatgc gatacggtgg
    10141 aataggcgag catacgtttg atattggtct gcatgatggc ggcaaggttg ccgaccagca
    10201 gcgaggcggc ggcaagcagg gcaaacatca gagaccagtc atgatgcacg gttcccagcc
    10261 cggtaacgag gatgcggaaa gcgaaaacga cggcggcgat tttcggggca gtgccgacca
    10321 aggcggtaac agaagtgggc gcgccgtgat acacgtcggg cacccacata tggaacggca
    10381 ccgcgccgag tttgaacgca acggcgacga caataaacac caaacccagt ttcaacagcc
    10441 attcattggc ttcttcattg aaggaagagg cgagcacgcc ggcaaattcc agcgaaccgg
    10501 ttgcgccgta aaccatagaa ataccgtaga gcagcaggcc ggatgccagc gcgcccaaaa
    10561 caaaatattt caaggcggct tcggcggcaa agccggaatc gcggcgcagg gcaatcaggg
    10621 cgtaaagggc aagcgacaag agttccaaac cgatataggc agttaaaaaa tgccccgcgc
    10681 tcaccatcac gctcatgccc aagagggcaa acagcgacag ggtgtaaaac tcgcctttga
    10741 aaataccgcg cacttggttg tagggcttgg cataaacaaa cagggcaaac gtcaaggcat
    10801 ataaaaccat ttttgccaaa cgcgacatac cgtctgcaat atacatgccg ttgaacgaag
    10861 acgtgctgcc ctgttcccaa acggcaagct gcaccacagc cgtaaccgcc accgttgcca
    10921 acgcgccgta atgcgtccaa cggcgtttgt catcactgac ccacaagtct gccagcaaca
    10981 ataacaccag cagcgacagc agcgcgactt cgggcatggc gggcattaaa ttcaaatcag
    11041 accagttcat ttacacacct caaatcttgc tttgtgccac atgggcaatc aaatcgtttg
    11101 ccgcctgatg caccacttcg ataaacgctt gcggatacag acccataccc aataccacaa
    11161 ccgccaaaat cgccagaatc gcaaattcgc ggcagttgat gtctttcatt tcggcaacgt
    11221 gcggattgtg gatcgcgccg taaatcacgc gcttgtacat ccacagggta taagacgcac
    11281 cgtaaatcaa ggtcatagcc gccagcgcgc cgacccagaa attcactttg accgcaccca
    11341 taatcaccat aaactcgccc acaaagccgg aagtcgcagg cagaccggcg ttcgccatac
    11401 cgaacaacat cataaacgcc gcaaacttag gcatcacatt gaccacgccg ccataatcgg
    11461 caatattgcg ggtatgcagg cggtcgtaca tcacaccgat gcacataaac atcgccgccg
    11521 acacgaaacc gtgggaaatc atctgaatga ccgcgccttt caatgcccag tcgtccaact
    11581 gcccgtcaac aaacaaaaac atcccaagcg ttacaaaacc catatggctg atggacgaat
    11641 acgccaccag ttttttcata tcggtttgca ccaaagccac cataccgata taaatcacgg
    11701 caatcagact caatacgatg atcacggggg caaaatagcg tgccgcatcc ggcataatcg
    11761 gcaggataaa gcgcaagaaa ccatacgcac ccagtttcag cgtaatcgcc gccaacacca
    11821 tcgaaccgcc ggtcggcgct tcaacgtggg catccggcag ccaagtgtgt acggggaaca
    11881 tcggcacttt tacggcaaac gacaggaaga acgccgcaaa caaaagctgc tgtacgccca
    11941 acggaatctg tttaatgttt tggaaatcga caatagagaa gctgcctgtt tgatagtaca
    12001 ggtaaaccat agcaaccagc atcaggagcg aacccatcag cgtatagagg aacagcttga
    12061 ccgacgcata gacgcggcgc gggccgcccc atacaccgat aatcaggtac agcggaatca
    12121 gcataccctc gaagaacaca taaaacagaa tcgcatcctg cgcggcaaac gcaccgttaa
    12181 tcaaacccga catcatcagg aatgccgcca tatactgcgc cgggcgtttc tgaataactt
    12241 cccaacccgc cagcaccacc aacagcgtaa taaacgcatt caagatgata aagagcactg
    12301 aaataccgtc cacgcccaat gcatagttga ttttcagcag cggaatccac tcgtggaact
    12361 cggtaaattg atagccgccg ctcaaacggt cgaaaccggt aaacaggggc agcgttacca
    12421 agaaaccggc aagcgcaccc ataaaggcaa gcacacgcgc cagcggcgca cggctgtcct
    12481 taccggtcga caaaaccagc acacctgcgg cgatgggtat ccatattgcc aagctgagta
    12541 ggtagttgga aaacatagtg gttaacctgt ggttaaaata aaaatatcgt agtgcgggtt
    12601 agtttgtcag acgacgtttc aggaggtcgt ctgaaaaagg gatgaatact ctctcccctc
    12661 tttactcact cccaatcaat atctgtacta tcgcgatatt ctgcctgcac taaaacatct
    12721 aattcagcca aattttttat ttctgactgc attgctagta aagctcttaa cttcatactt
    12781 aaatcaatat atctagagcc ttcacttgct ccttttgcag acttaaattt atttttttct
    12841 aaaaattttt taataggagc gtttaacact ggatatctat ctggatattc ttgacaaagc
    12901 atttcagaaa aaactgaacc acgagtggaa actccacatt cttttagcca atcaatttgc
    12961 ttggcaacaa cattatctct ctcagcaagt ggtgcatcaa aaactgattg gatagcaatg
    13021 cataattttc taaaatctga agactttccg gtccttttcc acgtttgatc ttgaaatcga
    13081 ttacctaccc cttctccttt attttcgctt ttccaagacc ataatttatt aaattccaca
    13141 taaaactcat cattgtctat ctttcctgca gcacattgct taataagact ttttaattga
    13201 ttacacacag tttgatggtt tctcatctgt tttctccgtg ctgcaatcag ttcttgatta
    13261 taatttggca tttccataaa taacttatcc atcaatgatt ggctaacagt tgatttctta
    13321 taattaattt cagcttcctg atactcctca agccaatctt ccgatactgg gatagatttc
    13381 atcgcaattt catcagccca agatttgact tttataaaat cctgctcgga aattttagtc
    13441 aaaatatttg cttcataatt gctattaaac gcagcatgag tcaaatttga agaaccgatt
    13501 aaagcataat attcattctt atcattcttc caaaatacag ctttaggatg aaaaccctga
    13561 atgccttgag ctaccatcag tgtgaatttc aaatgggacg gaagccattt caacactttc
    13621 cgaatagcct cttttttaga caatccgaaa tcttgtccaa aaataaattt aaactcttgc
    13681 aatttgctat tgagcttatt ttttacatcc caatgagtga gatacgcaga gaaaatatac
    13741 agctctactg cctctgaaac agctttctca tatattgaaa tcagttcctt ctcatttggt
    13801 gtatctgaat gtaaaaataa ctgtgtcatc ccttttgact cctctatatt tgttgagatt
    13861 gcctaaaaaa tttaggaaac agataatttg catgttggct atttcacgcc tgttgggtac
    13921 cattttgttc caccctttga cttaatgccc acttctcaaa cttttcagac gacctctaca
    13981 aaactatgaa caggtcgtct gaaactccgc ctttatctga acaatcccca gaaggtcatg
    14041 ccgagcagta ccaatacgcc gaataccata gcggcagcgt aagtgtagat aaagccggtt
    14101 tgggctttac gcacttgcgc ggcaatcgcg ccgaccagtt tggcagagcc gttgacgatg
    14161 ccgttatcga taatggcggt atcgccgact ttccagaaga aggtacccag tgcgcacgtg
    14221 cctttagcga agacgttgaa atacagggcg tcgaggtagt atttgttttc aaacaaaacg
    14281 taaaccggac ggaacgtctg tgcaattttc gctggcaggt gcggcagttt gacgtacaaa
    14341 agccatgcgc tcaacacgcc tgcgatagca aggtagagta cgggcgaatg caggctgtgc
    14401 gacaccattg ccaatgcgcc gtggaactct tccttcatga tgtgcatggt cggatgcgcg
    14461 tcggcgttga cgaaaatcac atctttgaag aaatcgccgt aaagcatagg ctcgatggca
    14521 acgtagccga tgatgacgga cggaatggca agcaaaatca aaggcagggt aacgaacagc
    14581 gggctttcgt gcggattgtc gtttttgccc aagccgtgat gttcttcgcc atgtccgtct
    14641 gaatggtgtt cgggcaggct gcgccatttc tcttcgccgt ggaacaccat aaagtattgg
    14701 cggaacgcgt aaaacgcggt aacgaacacg ctggcaagga cggcaaaata ggcaaagccg
    14761 ctgccgggaa gggtgctgta tttcgccgct tcgataatcg aatctttgga gtaaaagccg
    14821 gagaagaacg gcgtaccaat cagcgacaag ttaccgatca acatggtcaa ccaagtaatc
    14881 ggcatgtatt ttttcaggtt gcccatgtgg cgcatgtctt ggtcgtgatg cataccgata
    14941 atcgcgctac ctgcggccaa gaacaacaag gctttaaaga aagcgtgggt catcacatgg
    15001 aacatcgcca cggaataggc agacgcgccc agagccacgg tcatgtagcc cagttgcgac
    15061 agggtagaat acgcaaccac acgtttgatg tcgttttgaa tcacgcccaa aaaacccata
    15121 aacagggcag taatcgcacc gattaccatg atgacagaca gcgcagtgct gctcatctca
    15181 taaatcggcg acatgcgcga caccatgaac agacccgccg taaccatcgt cgcggcgtga
    15241 atcaatgcgg aaatcggggt cgggccttcc atcgaatcgg gcagccagac atgcagcggg
    15301 aattgagccg atttacccat tgcgcccaca aacaagagca aacaagttac ggtaatcaaa
    15361 gaccattcca caccggggaa aagctggatg gtggcatttt ggacgttggg cagataggcg
    15421 aatacgtcct gatagcgcag gctgccgccg aaataggcaa gcaccaagcc gataccgagc
    15481 aaaaagccga agtcgccgac acggttgatc aaaaaggctt tcaggttggc aaatgttgcg
    15541 cttggacgtt tgaaatagaa accgatcaag agatacgaca ccaaacccac agcttcccag
    15601 ccgaagaaga gctggatgaa gttgttgctc ataatgagca tcaacatgct gaaagtaaac
    15661 aaagaaatgt agctgaagaa gcgttggtaa ccgacttttt catcgtgcat atagccgatg
    15721 gtatagatgt gtaccatcaa cgatacgccg gtgaccacga ccatcatcat cgccgtcatc
    15781 gtatcgacca agaagccgac ggagaaatcc aaaccgccca tcgtcagcca ggtatagacg
    15841 ttttcgtcaa acttggcgcg gctgccgtcg ataaagcccc acagcacata agccgacaac
    15901 acggcagaca ccgccacacc gagtatcgta accgtatgcg cgccggcacg tccgattttg
    15961 ttgccgaaca aacccgcgat cagtgagcct gccaaaggca caagggcaat gatgagatat
    16021 aaagtcatgt cgttcatttg attgaatccg attgatttaa aaatctatgt ttgtttcgta
    16081 caaaattact tcggaaaaac aaatccaaca cgctccaatc gtttgcgtgc cacagctaat
    16141 tgctcttcag taaataaatc acaccacggc ttttgtaaca ccagatattc catactgtat
    16201 ttcaaggcgt ggactccgcc accactcaaa atcagctctg taaaaccggt ctgagtcttc
    16261 ttttcccccg tactcaataa tttatccgcc gcctctttac caccaaattc atttacaatt
    16321 tgtaaaaatc gtgtcgcctt gtaaggttgc ggcaaattca aagcctcctg ataaatattt
    16381 aacatggctt tatgaaattc ttgttctaac tgatttttat ccatcattct tcttccaata
    16441 tttcagaccg gattattctt acccagaatt tcttttctca tccgctcccg tctgatcacc
    16501 taccaaatca ggtcgtctga aacaatctga aatcgctttt cagacgacct tcagcctttt
    16561 tcataccctt cgtaataata tgactgctcg atacctttaa agatgatttc acggttgtcc
    16621 acatcgtcgg tcaggttgtc ctttaacaaa aagcgcagtt ctaaatcgtt gacggggctg
    16681 cgttccatcg cctgcaaata cagggttttg cttacatttt gccagttcac gacttttttc
    16741 aggtttttct tcagcaccaa atccagccag atgcgggtac ttctgccatt accctccaaa
    16801 aacggatggg caatgttcat ttcaacatat ttggcgatga tttcttcaaa agtccgctcg
    16861 ggcatctgct cgattttaac caaagcctct tttaaataca tggcgttggc aaaacgaaaa
    16921 ccgcctttgg aaatgttgtc ttccctgatt tgacccgcaa aatcatataa gccgccgaac
    16981 aggtaacggt gaatctgttg caggcccgcg gtggtaccga cttcgatacg gtcgatgtcg
    17041 ccgctttcaa acaggcggcg ggcattatgc aggctttgtt cgtctatgga tttcatcgtt
    17101 tttcctatcg ggttttcaga cggcatcggt gtcttgtcag gtctcgaccc aacctacctt
    17161 taccctttca acccatccaa atcggcaaca ttaatcgttt gtcggttgcg gtacaccaat
    17221 accataatcg ccaaaccgat ggcggattcg gcagcggcaa cggtcaatac gaagaatacg
    17281 aaaatttgtc cggcagtatc gcccaaatgt tgcgagaagg cgataaagtt gaagttcacc
    17341 gccaagagca tcagctcaat ggacatcaac aataccagca cgtttttgcg gttcataaag
    17401 atacccattg cgctgatacc gaacaggagc gcacccaata ccagataatg cgtcaaggta
    17461 atcatgcttt gccctcccct tccggcttga ggccgtctga aacttcgctt tcttcggcag
    17521 attcgacttg cggtttgacc gcttccattt tcaccagacg catacggcct tgatcggcgc
    17581 gtactttaac ttggtctgca gggtccatac gtttcggatt aaccgtttta cggtgaacca
    17641 gcgcaatcgc cgccaccata cccaacagca gcaataccgc agccaattca aacggcaaca
    17701 gatagtcggt ataaatacgg ctgcccaaat cgcggatatt gttgtaatcg gccggaatgt
    17761 ctttcatcag gccaaatgcg gcaaggtcgg ttttcgggtt gaccaaaatc agaatcaggg
    17821 caaccgccaa caacgtgccg accacaccgg caacaggcgc atgccgccag aaaccggcac
    17881 gcatttcttc aatgtcgatg ttcaacatca tcacgacgaa caggaacaac accatcacgg
    17941 cgccgacgta aaccaccacc agcgtaacgc ccaaaaactc agcctgcatc agcatccaaa
    18001 gcatcgcgct cacgcagaag gtcagcacca gatgcaaagc ggcgtgaacg gggtttttag
    18061 cgatgacggt tttgagcgcg ccatagagaa ttatcactgc aaaaatataa aataaaatca
    18121 gttggaaagt catagtctat gctttcttta ttaatcaaaa atatggtttc aagcagtctg
    18181 aaaatcctgg ttacgcaaac aatcaaactt aatcagttga tccatatccg cacaaatcca
    18241 cgttgtcgca attttgaata acaacatttc tatgtgatac ggttttgctt gtttaccata
    18301 aacttgtttc gccaaatctt gtaacaatcc acaatattga tgaaacgaaa cgtctggttt
    18361 ccgataattt ggtttgatat cagaaaaatg aaacaatacg ttcatatttc ggatttctag
    18421 ttccctattt ctaccttgtg gctgaaaaaa caaatctgca tttgtaaatt taaacaacaa
    18481 ccaattcaac gaaaaaatag cgcgtgaatc ataaatggcg aatcgctcac aatctataaa
    18541 agaagccaat ttagataatg atgaaatttt caacaaacca atactcaatt ttcccgaatt
    18601 aagttccgat aaaaattgac gaataagctg gctattttca gcagattgtt taaaactttt
    18661 aatcccaccc cattcacgga taatccaata atgcgtcttc aagctatcat cattttgtaa
    18721 ttttggggat aaattttctt ttaaaaaaat attttgttca aaattactta atccacttgg
    18781 caactcaatc ggactatttc taattctaat ttcttcaaaa ttatctattg gctcatgttt
    18841 ggctaaatat ttcaatgcat taatcagtcc ttccatattt aaagccttcc aagaagccca
    18901 aaagcattaa cgatacggcg cgtcagcggc tttacgtttg gcgatttcgg cttcgtattt
    18961 gtcgccgatt gccaagagaa tcggcttggt catgtgcaag tcgccttttt tctcgccgtg
    19021 gtattcaaaa atatgggttt ctacaatcgc atcggtcggg caggcctctt cgcagaaacc
    19081 gcagaagatg cacttggtca ggtcgatgtc gtaacgcttg gtgcggcggg tgccgtcttc
    19141 gcgttcttcc gattcgatgt taatcgccat tgccggacac accgcctcgc acaatttaca
    19201 cgcgatgcag cgttcctcgc cgttcggata ccgccgctgc gcgtgcagac cgcggaaacg
    19261 cacggattgc ggcgttttct cttcgggaaa ataaattgtg tctttgcggg cgaaaaagtt
    19321 tttgagcgtt acgcccatgc cttttaccaa ctcgccgagc agaaaggttt ttactaagtt
    19381 agccatatta tgttccctca aaacagggat ttcgttaggt attcaaaatc gctttgttca
    19441 gacggcctca agatgccgtc tgaaacttat ttccacaaat tcagcggtga aatcatccac
    19501 acgcccaaaa tcacgatgta ggcgaagccg atcggaatca gcactttcca gcccaaacgc
    19561 atgatttggt cgtaacggta gcgtgggaaa gttgcacgga tccacagata ccagtacagc
    19621 accgccgcca ttttcacgaa catccagaat gcggaaggcg taccgataaa gccccagctt
    19681 tgcgggaacg gagacagcca gccgccgaga aacatcagcg atgtcagcgc ggcaatcaga
    19741 atcatgaaaa tgtattcggc aaggaagaac agcgcgaatg cgaagccgga atattcaacg
    19801 tggtgtccgg caacgatttc ggactcgccc tctgccacgt caaacggtgc gcggttggtt
    19861 tcggcaacgg cggaaatcag atagacgatg aagattggga acagcggcag ccagttccac
    19921 gagaatacgg aaccgcccgc gatacctttt gcctgcgcgg caacgatgtc ggagaagttc
    19981 atgctgcccg acaccatcac gacacacacc agcgcggcac tcatggcaat ctcgtaggaa
    20041 atgctttgtg cggaagcacg cattgcaccc aagaacgaat atttggagtt ggaagcccag
    20101 cccgcgatga tcacgccgta aaccgacagc gaggtaatca tcagaatgta caaaagaccg
    20161 atattgatgt tggtcagcac ccattcttcg ttgaacggaa tcactgccca tgccgcgaaa
    20221 gacggagcaa gcgacataat cgggccgata tagaacaggg ctttgtttga cagcttcgga
    20281 cgggttacct ctttaaacaa gagtttgaac acgtcggcaa acggctgaat cagaccccat
    20341 gggccggtta cgttcggacc gacgcgaagc tgcataaagc cgatgacttt acgttcaaaa
    20401 taagtcaggt aggcgacggt cagaataagc ggaatcagaa taatcacgat tttgacgatg
    20461 acggatacca ccaagcctac ggtgatgccc aaatcgccca gaccgagcgt tgcggcaaag
    20521 aggttttgga accattcctg cataatcaag ctcccgccag ttcaatagtg tccatcaacg
    20581 cacccagcgc ggcattttcg gtatgcagcg gcagatgcac cacgttttca ggcaaaccgg
    20641 catcggcttt gactgcaacc gatacgcctg cgccgttttg tttggcgacg gcggtttgtc
    20701 cgtcttgcag gcccaagcgt gccaatgtgt tcggatttac acgcgcggca ggcacggcgg
    20761 catggctggt ttcttgcaac ggtacggaac ggcgcacgat ggcatcggtg tgatagatgc
    20821 ccactccgcc cacgcgcacg aggcggcttg atgccgtctg aaaatctttt tgagtcgaag
    20881 acacgcggtt gtccagtttg gacggcaggc tttccgcgtc cagcgcgtct ttcagaatcg
    20941 cagcggtatc gtgatattca aaacctttca ggtcaaacag gttgcccaat acgcgcaaca
    21001 ctttccacag cggacgcgag tcgccgaagc cttgtaccac gccgtggaag gattgcagac
    21061 gaccttccat attgatgaag ctgcccgagg tttcggtaaa cggcgcaatc ggcaacaaca
    21121 cgtcgcacac atccagcagc gtttcgctga caaacggtgt aaacgccatc acgcttttcg
    21181 cctgtttcaa cgcggccacg gcttttgaac cgtcaaccgt atcgatttcc ggctcgacgt
    21241 tgagcagcaa gactgcctgt ttcggcgcgt ttgccatttc ggtaacgctc tcacctgagt
    21301 tcacgcccaa tacgtccgca ccgacgctgt tggcggcttg cggcaaaata cccagtaccg
    21361 cgccggttgc atccgccagc tcttgcgcgg cggcataaat ggcggcgtaa tcaggatggt
    21421 tttgcacttc cgcgcccaaa atcaccgctg ctttttcagc gtttttcagg ctggcggtaa
    21481 cggcgtgttc cgcattggca gacaggtttt tcagacggcc tgcccactcg tcgggatgtg
    21541 cggcttcttg agagagaagc ggcatcaaca actcttcttt actgccggcc aatacactca
    21601 acgccatacg gtctttggcg gcgcggcgca ggcgggcggt caggagcggc tgttctttgc
    21661 gcaagttcgc gccgactacc aatacggcat cgttgtcagc caaagattca atgctttgtc
    21721 ccaaccattg cgcaccttta atgccgtctg aaagacgttt gtcttgttgg cgcagacggg
    21781 tggcaaagtt tttaacaccc aaaccgtcgg cgagtttttt ggccagatac agctcttcaa
    21841 ccgtattcat cgggttcgcc caaatgccga cttggttttg gttgccgtct ttggcgatac
    21901 attcaatcgc gctgcggaca tattccaacg cggttttcca atccacgtcc atccactcgc
    21961 caccctgttt gattttcggg tttttcagac ggctttcgtg atacaggcct tcataggcga
    22021 aacggtcgcg gtcggacaac cagcattcgt tgatggcttc gttttccaac ggcaacactc
    22081 ggcggacggt atggtctttg gtttgcacaa tcaaattgct gcccaaagcg tcatgggcgg
    22141 aaacggattt gcggcggttc aattcccaag tacgcgcgtt gaaacggaat ggtttgctgg
    22201 ttaatgcgcc gacaggacac aaatcgatga cgttgcccga caattcggtt tccaccgctt
    22261 tgccgataaa aggcatgatt tcggaatgtt cgccgcgatt cgccatcgcg atttcctgca
    22321 aaccggcgat ttcttcggtg aatcgcacgc aacgggtaca gtggatacag cggctcatct
    22381 cctcggcgga aaccaaagga cccatgtcct tgccgacgac ggaacgtttt tcttcggtat
    22441 agcggctggt ggttttgccg tagcccaccg ccaaatcctg caactggcat tcgccgcctt
    22501 ggtcgcaggt cggacaatca agcggatggt tgatgagcag gaactccatc acgccttcct
    22561 gcgcctcccg cgccttcgcc gaatgcgtgc gcacaatcat gccgtccgtt accggcgtag
    22621 cgcaggcggg cagcggtttg ggggcttttt ccacgtccac cagacacata cggcagttgg
    22681 cggcgatgga aagttttttg tggtaacaga aatgcggaat ataagtaccg agcttgtgcg
    22741 cggcttcaat gaccgtcgcg ccctgctcca cagatacctg tttaccgtcg atttcgattt
    22801 gtaacatggt tcgttcctag ttacgggtat ttgataaata atcttttatg gaggtcgtct
    22861 gaagaatggg tttcagacgg ccttttgagt ttcattgaca atcaatactg tttaaaacgt
    22921 ttactgccac cttgccgtca ttcccgcgta ggcgggaatc cattcttggg ttttcggtaa
    22981 ctgttttcaa acattggttt cttaactttt gcggtgattc ccgccgtagc ctgtctgcgc
    23041 gtaggtgggg ctggaataac gacagttttt aaagttccag aaataaaact aaacaaacaa
    23101 cacgcccgac aagtccaacc attcaggatt ttgttcttca atcagtttaa tcttccattc
    23161 acgtcgccat tttttcatcg ttttttcttt ggcaactgct tcaggcatat tctcaaaaaa
    23221 ctgataccaa actaaatcat gcacatcata tcgagcagta aagccatcaa tattgacatg
    23281 gtttttgtgc tgccaaaccc tttccggcaa attcatggta acaccgatat agagtgttcc
    23341 atttttaccg tttgccaaaa tatatacggc aggttgtcta gtaagcggat ggtttcgtat
    23401 tcatttttcc tgcaatataa acaacaactt ttttaatatc atttacaacc gttttcatct
    23461 gaaaacatct gcctact
//
LOCUS       DAXIEB010000018        22423 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000018 DAXIEB010000000
VERSION     DAXIEB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.018"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            200..1561
                     /locus_tag="ACFOA3_000622"
     CDS             200..1561
                     /locus_tag="ACFOA3_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6897671.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            1734..2057
                     /locus_tag="ACFOA3_000623"
     CDS             1734..2057
                     /locus_tag="ACFOA3_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897672.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(2174..3127)
                     /locus_tag="ACFOA3_000624"
     CDS             complement(2174..3127)
                     /locus_tag="ACFOA3_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6897673.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(3266..4288)
                     /locus_tag="ACFOA3_000625"
     CDS             complement(3266..4288)
                     /locus_tag="ACFOA3_000625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6897674.1"
                     /translation="MYFFPRFPFIDIDRIRWVRVGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTKHSSTITRGLIRPHQWQPMKNAAAHAAARLAISRHFAH
                     VLQHKYGCEWQYLPNILGGIFNRPFEQREKNNKPHFVFCTVSHLRRLKGHDVLLTAFA
                     RALAQCPQLRLNIGGSGQEEQRLKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFV
                     LASRTETFGVVYIEALSQGLPVIATRCGGAESIVSDGNGYLVPVDDDDALADALIKMY
                     EHHSDFEPARLRENCLNEFGENAVIGRLIGIFRQAIAEYGKKIPVKYSGLTKIRTRRR
                     SRRQYK"
     gene            complement(4441..4616)
                     /locus_tag="ACFOA3_000626"
                     /pseudo
     CDS             complement(4441..4616)
                     /locus_tag="ACFOA3_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            4762..5466
                     /locus_tag="ACFOA3_000627"
     CDS             4762..5466
                     /locus_tag="ACFOA3_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6897675.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(5530..6096)
                     /gene="dcd"
                     /locus_tag="ACFOA3_000628"
     CDS             complement(5530..6096)
                     /gene="dcd"
                     /locus_tag="ACFOA3_000628"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6897676.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6162..6578)
                     /locus_tag="ACFOA3_000629"
     CDS             complement(6162..6578)
                     /locus_tag="ACFOA3_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6897677.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(6640..7539)
                     /locus_tag="ACFOA3_000630"
     CDS             complement(6640..7539)
                     /locus_tag="ACFOA3_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6897678.1"
                     /translation="MWFKQISFYPLNKEKLPEADILADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(7570..9027)
                     /gene="der"
                     /locus_tag="ACFOA3_000631"
     CDS             complement(7570..9027)
                     /gene="der"
                     /locus_tag="ACFOA3_000631"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6897679.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(9181..9810)
                     /locus_tag="ACFOA3_000632"
     CDS             complement(9181..9810)
                     /locus_tag="ACFOA3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6897680.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(9811..11106)
                     /gene="hisS"
                     /locus_tag="ACFOA3_000633"
     CDS             complement(9811..11106)
                     /gene="hisS"
                     /locus_tag="ACFOA3_000633"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6897681.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTCAYG
                     YRQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIRDYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQDFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTPTLQQWKNA"
     gene            complement(11233..11889)
                     /locus_tag="ACFOA3_000634"
     CDS             complement(11233..11889)
                     /locus_tag="ACFOA3_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6897682.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRVQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVEQIRQARAE
                     "
     gene            complement(12050..12337)
                     /locus_tag="ACFOA3_000635"
     CDS             complement(12050..12337)
                     /locus_tag="ACFOA3_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897683.1"
                     /translation="MDDLIVAFLGYLSGKYSEGKISRLEISLTAGSIFFVVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(12541..12756)
                     /locus_tag="ACFOA3_000636"
     CDS             complement(12541..12756)
                     /locus_tag="ACFOA3_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897684.1"
                     /translation="MVNKYIKTAISITAISIFYLLTQESSGKTEEPSYFLMFMFLNSL
                     WFEENKTVMAAVTAMIAAHLIFVTLSD"
     gene            complement(12746..13141)
                     /locus_tag="ACFOA3_000637"
     CDS             complement(12746..13141)
                     /locus_tag="ACFOA3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897685.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAWGPVGAFIGGVGGGIGYMMNQQLSGSRFSPTAFTLAVGAGAFGGA
                     TAGPAGVVWGFNRSLATGMVTGVAQRYGK"
     gene            complement(13184..14053)
                     /locus_tag="ACFOA3_000638"
     CDS             complement(13184..14053)
                     /locus_tag="ACFOA3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6897686.1"
                     /translation="MKRIFLSALPAILPLSAYADLPLTIEDIITDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDDKNPALIGFLEGTLYEKSRGKA
                     SSGKSWLIGATTYKAIDPVVLSLTAAYRINGSKILSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGRQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            14218..15141
                     /locus_tag="ACFOA3_000639"
     CDS             14218..15141
                     /locus_tag="ACFOA3_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6897687.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(15219..15860)
                     /locus_tag="ACFOA3_000640"
     CDS             complement(15219..15860)
                     /locus_tag="ACFOA3_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6897688.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLSVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQERIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15915..16706)
                     /locus_tag="ACFOA3_000641"
     CDS             complement(15915..16706)
                     /locus_tag="ACFOA3_000641"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6897689.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            16872..16947
                     /locus_tag="ACFOA3_000642"
     tRNA            16872..16947
                     /locus_tag="ACFOA3_000642"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16905..16907,aa:Thr,seq:tgt)
     gene            17089..17880
                     /gene="panB"
                     /locus_tag="ACFOA3_000643"
     CDS             17089..17880
                     /gene="panB"
                     /locus_tag="ACFOA3_000643"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6897690.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYGSSFAALMDDAGVEMLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGSKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHNSVQAAVRAYVEEVKAKTFPAAEHIFTD"
     gene            18003..18839
                     /gene="panC"
                     /locus_tag="ACFOA3_000644"
     CDS             18003..18839
                     /gene="panC"
                     /locus_tag="ACFOA3_000644"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6897691.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLSRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            19004..20842
                     /locus_tag="ACFOA3_000645"
     CDS             19004..20842
                     /locus_tag="ACFOA3_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897692.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALRYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            20982..21563
                     /locus_tag="ACFOA3_000646"
     CDS             20982..21563
                     /locus_tag="ACFOA3_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6897693.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            21573..22418
                     /gene="ispE"
                     /locus_tag="ACFOA3_000647"
     CDS             21573..22418
                     /gene="ispE"
                     /locus_tag="ACFOA3_000647"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6897694.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
BASE COUNT         5215 a         5272 c         6314 g         5622 t
ORIGIN      
        1 ccgccacaaa agcagggtct gattttttta aagggcaatc tgttataatg gcgcgttgcc
       61 gccgcgaggg cggcgtgatt cggacggcgt agtttctacg ccttttgttt atggttacgg
      121 catcttgcgg aatcgtaccg cctgatgccg tctgaacacg gttgcccgtg gagatgccgc
      181 tcttcgggtt agaatattta tgctgaaaaa atggttgaat aagatgctgc cttccggtcg
      241 gagcagtaaa aaagcggaaa gtaaaacgat cattcctgcc gaaagacaca acatccgtgc
      301 cgaaatgttg agctttgccg ccgaaaacgt catacgccgc ctgaaagggg cggggtttca
      361 ggcttatgtg gtcggcggcg cggtcaggga cctgctgctc ggcatcgaac ccaaggattt
      421 cgatgtcgca accgatgcca cgcccgaaca ggtgcacaaa ctcttccgcc gcagccgcat
      481 catcggcagg cgttttcaga ttgtccatgt gatgaacggt gcggagatta tcgaagtaac
      541 gacgtttcgc ggcggtgcga aggttcatca gaacgcgcac ggcagaatca tgaaggacaa
      601 cacctacggc agcatcgaag aagatgcgat gcggcgcgat tttacctgca atgccttgta
      661 ttacgatcct gaaaaagaag agattttgga tttccacaac gggattgccg atgttgccgc
      721 ccgcaggctg gttatgattg gtgatgccgc cgaacgctat caggaagacc ctgtcaggat
      781 tttgcgcgcc atccgcctgt cgagcaaatt gggctttgat ctgtcggaag aaaccgccgc
      841 accgattgcc gaatcgatat gccgtctgaa gcacgaaccg gtagcgcggc tgttcgacga
      901 aattatgaaa ctgctgtttt cagggcacgc gcgcgagtgt ctgaaacgtt tgaacggatt
      961 tgacataccg gacgacatcc atccgctgct caatgccttg cgcgtttcag acggcatcgc
     1021 cggaaaaatg acggtgcttg ccctgaaaaa taccgatgag cggctgcgtg ccgacaaatc
     1081 ggtttcggtc ggtttcgtac tggcggccct gatgtggccc gagttggaac gccattggaa
     1141 aagcaatctg caacagggtt tgaaacccgt gcccgccctg tccgatgcaa tcaatacgat
     1201 gcgcgaaacc gtcgaacgcg gttggggcgt accgcaacgc ttttccgcca cgatgcgcga
     1261 aatttggatg ttccagccgc agtttgaaaa ccgcaaaggc gcaaggccgc acaaactgtt
     1321 tgcacaggcg cgtttccgtg ccgcctatga tttcctgctc ttgcgcgccg aaaccggcaa
     1381 tgcggaccgc gcccttgccg agtggtggac ggcgtttcag acggcatcgc aggaacaacg
     1441 gacggaaatg accaaaaacg aggctgctgc ccggcacgaa aaaaacgaag gacaggcgaa
     1501 aaaacgccgc cgtcgcaggc gcaaacccaa gccgaaggtt gagggaacgg attgggaata
     1561 agggtcaaca gacaaggagc aatgaagttt caacacatgg gatgaagcat aaagtgccgt
     1621 tctatgtatt atcctgattt gtaaggggct tcatccccgt aaataaagtc taaccctgcc
     1681 tctcggaaaa aggatgtccg ggtgggcagg gttcaagaaa caaggaaaaa ttgatgaaaa
     1741 aatgtatttt gggcattttg accgcgtgtg ccgccatgcc tgcatttgcc gacagaatcg
     1801 gcgatttgga agcacgtctg gcgcagttgg aacaccgtgt cgccgtattg gaaagcggca
     1861 gcaataccgt caaaatcgac cttttcggtt caaattccac catgtatgta tgcagcgtta
     1921 cgccttttca gaagacgttt gaggcaagcg atcggaatga aggcgtggcg cggcagaaag
     1981 tgcgtcaggc gtgcaaccgc gaaacttcgg caatgttttg cgaagatgag gcaatccgat
     2041 gcagaaaatt cgattgatgt atcggttgga cggataaaga aacggatacg gatacggata
     2101 cggagcttgg cttccgtatc tgtttttctc tgcccgattt tccatgcatc gggtttcaga
     2161 cggcattgga atgtcagtcg tgttctgccg attcgtaggc ttcgacgatt ttttgcacca
     2221 aaggatgccg gacaacgtct tcgccggtaa aggtgtggaa atacagccct tccacgttgt
     2281 gcagtttctc acgcgcatct tttaatcccg atttgatgtt tttgggcagg tcgatttggc
     2341 tggtgtcgcc ggtaatgacg gctttcgcgc cgaagccgat gcgggtcagg aacattttca
     2401 tttgttcggg cgtggtgttt tgcgcttcgt cgaggatgat gtatgcgccg ttgagcgtcc
     2461 tgccgcgcat ataggcgagc ggggcgattt caatcaggcc tttttcaatc agcttggtta
     2521 cacggtcaaa gcccatcagg tcatagaggg catcataaag cggacgaagg tagggatcga
     2581 ctttctgggt caggtcaccg ggcagaaaac ccagtttctc gccggcttcg acggcaggcc
     2641 gcactaaaat gatgcgttcg acttggtgtt tttccatcgc atcgacggcg gcggcgacgg
     2701 cgagataggt tttgcccgta cctgccggcc cgagaccgaa tacgatgtcg tggttgagta
     2761 gggcgcggat atagccgttc tgccgtggcg ttctgccgcc gatgctgccg cgcttggtgc
     2821 ggaaataata ggcgtggtca tggttttttt cttgatgacc ggcatcttcg gtttgggctt
     2881 cgacggcggc aagcctgatg tcgccgtcgt ttaggtcgcg cgtctgcgcc gtttccaaga
     2941 gtttgagcag tgcgcgtttg ccggcgtgtg caaatgcgcc gttgaaagtg aaatgttcaa
     3001 aacggcggct gatgtggata tcgagtgctt tagcaagtaa atcaaggttg ttgtcaaaag
     3061 aaccgcacag acgctgcaac gccaagttgt cggtttcttc taaatgcagg tggacggtat
     3121 gtgtcatatg aaggtccgaa tagttggata ttgtgtgatt ttaatctata gtggattaga
     3181 tttaaaccag tacagcgttg cctcgcctta gctcaaagag aacgattctc taatgtgctg
     3241 aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     3301 tgatttttgt taatccacta tatttcaccg gtattttctt accgtattct gcgattgcct
     3361 gtcggaaaat gccgatcaac ctgcctataa cggcattttc gccaaattcg ttcagacagt
     3421 tttccctaag tcgggcaggt tcaaaatcag agtggtgttc atacattttg atgagtgcgt
     3481 cggcaagggc atcgtcgtcg tcaacaggaa ccaaatatcc gttgccgtct gaaacaatag
     3541 attccgcacc gccgcagcgt gttgcaatga cgggcaatcc ttgggacagt gcttcgatat
     3601 agaccacgcc gaaggtttcg gtgcggctgg caaggacgaa tgcgtcgctg ttcctcatca
     3661 aatccaagac tgcttcgggc tgcaatgcgc ccaaaaatgt aacggcatgg gtaatgccca
     3721 agtctgccgc ctgctgtttc agccgctgtt cttcctgtcc gctgccgccg atgtttagac
     3781 gcagttgcgg gcattgtgcc aacgcccggg caaaggcagt gagcaggaca tcgtgtcctt
     3841 tgagacggcg aaggtgcgag acggtgcaga acacgaaatg cggtttgttg tttttttctc
     3901 tttgttcaaa cggtcggttg aatattccgc ccagtatgtt ggggaggtac tgccattcgc
     3961 agccgtattt gtgttgcagg acgtgtgcga aatggcggct gatggcgaga cgtgcggcgg
     4021 cgtgcgccgc cgcatttttc ataggctgcc attggtgggg acggatcaga ccgcgcgtga
     4081 tggtgctgct gtgtttcgtg acgacatagg ggatgccgta tttttgggaa attttttggg
     4141 cgagtatgcc ggcatagttc atgcggtggg cgtgaatcag gtcgggcagc ccgttttcgc
     4201 ggatgtagtg tttgaaggct ttcaaaccca cgcgcaccca gcggatgcgg tcgatgtcga
     4261 tgaacggaaa gcgggggaag aaatacatgc cgtgccatgc atagatgtcc aaaccgcttt
     4321 gccgatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat
     4381 agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat attttgcaaa
     4441 accgtaaggg tcggtcagga tgcttgctgt ttctttccgc aagtagcgga acatcggtgc
     4501 aagcacggcg gttttgatgc ctttcctctg gaatgccagt gcctgatttt gaaaaaaatc
     4561 ccgtccacat cctgttcgga ttgcggatac catgagggga tgacgaggac gtgcaagggt
     4621 tcgggcatag tgggattccg tatcggaaag gcggttatta taagacagac gcagaccgaa
     4681 tatttaaatt gttgccttac gctaatgcaa tttagcgcgc cgatatgcta gattggcggt
     4741 tttatgagtg aggatacgga tatgttgcgt tctattttgg cggcttccct attggcggta
     4801 tcttttccga cggcggctga agcattgaat tacaatattg tcgaattttc tgaatcggcc
     4861 ggtgtcgagg cggttcagga tacaatgtcc gcgcgtttcc aggtggcggc ggaaggacgg
     4921 gacaaaaatg ccgtcaatgc cgagtttgtt aaaaaattca acagttttaa cagaaaatcg
     4981 aaaaacggca gctttaaaac cgaattggta tcgcgcagtg cgatgccgcg ctaccagtac
     5041 accaacggca gacgcattca aacaggctgg gaggagcgtg cggaatttaa ggtcgaaggt
     5101 agggattttg atgcgttgaa ccgttttatt gctgatgttc agacggatgc ttcgcttgaa
     5161 tataccgatt tcagcgtgtc gcgtgaacgc cgaaacgagg tcatcgatca ggtcagcaag
     5221 gatgctattt tgcgttttaa gacgcgtgcc gaaaaactgg cgggcatttt gggtgcgtcc
     5281 ggttataaaa tcgtcaaatt aaatttggga cacatcggca gccatatcgc gggaggggga
     5341 gctgctcagg caaaaatgct tcgtgccatg ccgatggcgg caagcgtcaa tatggagggt
     5401 gcggattctg ccgcgcaggg tgtggaggaa atcagcatca gcgtcaatgg gacggttcag
     5461 ttctaaccac ggataaacag gtagatgccg tctgaaaccg gatgatacgg ttcagacggc
     5521 atttatattt caggctttag gcagggtaac gccggtttgc cccatatatt tgccgttgcg
     5581 gtctttgtag gaagtttcgc agatttcgtc gctctcgaaa aacaggactt gcgccacgcc
     5641 ttcgcctgcg tagattttgg cgggcagggg ggtggtgttg gaaaattcga gggtaacgta
     5701 gccttcccat tccggttcga acggggtaac gttgacgata atgccgcagc gggcgtaggt
     5761 ggatttgccc aagcagacgg tcaggacgtt gcgcgggatg cggaaatatt cgaccgtgcg
     5821 cgccagtgcg aaggaattgg gcgggatgat gcagcagtcg tcttcaacgg taacgaagtt
     5881 tttcgggtcg aagtttttgg gatcgacgat ggtgctgttg atgttggtga agattttaaa
     5941 ttcgtttgcg cagcggatgt cgtagccgta gctggacgta ccgtaggaga tgatgcgttt
     6001 gccgtcggct tctttgattt ggttcggctc gaaagggtcg atcatgccga attcttcgct
     6061 cattcggcgt atccatttgt cggacttgat gctcataatg ttttccttgt ttcttgcagt
     6121 gttcggacaa agcattgggg gatgccgtct gaaaacgggg cttatttgtt tttgggcagc
     6181 ttcacttctt taatcatgcc gttttcgcat ttcataatga agagggtttc gccgttttgc
     6241 gagacggtaa atctgccgtg tgccaagcct tttttgaacg tgcccgagag taccatgttg
     6301 cggaatttgg tactgtcgga attgaacggt tcgataaata tttcgcggtt ggcggcaacg
     6361 gtataaacgc cttgtccgtc gaatttgcca tttttaaacg aaccggtata accgcgcccg
     6421 tcccggcaac gccatatgcc tttgcctgaa ggaagcccgt cttttccgac tccgccgtcg
     6481 taggtgcagc cgggttcttg ataggaagtc agaacggcgg ccgaagtggg gagggcgaac
     6541 atcatggcgg gcagtaggaa tgcgagatgt ttaagcataa gggttattcc attggatttt
     6601 ggttgacggt attttgtcgt gaaaaagccg tctgaaaaat caatcttgcc agccgcccaa
     6661 ataggaaacc agttcttcca acatggtgct gacggattcc gccatcagaa tttgcgaggc
     6721 gaaggcaagg ccggcggcat cgtcgccgtt gctttcggct tcttcctgca atacgtcgag
     6781 gtattggatg cgcttgagtg tgaagtcttg agtgaggata aaggcaattt gttcgcgcca
     6841 caccaagccc agttgggtaa cggttttacc gtttttgacg tgttgaacca cttcgtcggc
     6901 ggttaaatct tgtttggata ctttgacgac gggaacaata tcgcccgtac ctttgagttc
     6961 gcaatcgctg tctaattcaa aaccgccttc gcaatgccct tgcaacagcc agccggtcat
     7021 caaggaagag ggcgattgct tggtattcgg cagcgaggct tccaaaccgc ccaaagcttc
     7081 gcgcagcttg gtcaggatgt tttccgcttt ggcggaagcc gcgttattga cgagcaggta
     7141 gccgtggcgg gtgttaaaca ctgcttctgt acggctgctg cgggtaaacg ctcggggcag
     7201 caggtcgtct gtaatttgct ctttaagctc ttgtttttct ttgcgaccga cattgcgggc
     7261 ttcattgttt tggatttccg ctaccttctc ttccaaaata tcgcggatga cgccggcagg
     7321 caggactttt tcctcttttt tcagggcgac gcgcaaggta aagtcggcag ggaaaacgag
     7381 ttcaggggag aatgaaaccg gtgcggtaaa gccttcgctg aaccagtcta agccttggca
     7441 atgggtaaat tcagcttcag caagtttgtc ggcaagtatg tccgcctcag gcagcttttc
     7501 tttgttgagc ggataaaaac taatctgctt gaaccacata atgtttccta ttgtttgaaa
     7561 tgtcgggaat tatttgctga attgtttttt cacactgact ttggttttct tcttgaagcg
     7621 gtttttctct tctttgcggc cttcgcgttt ctcaatacgg ttgctcaggc tgacgcggcg
     7681 cagcggtttt ttcttcggtt tgtcttccgc attttcatac gggttttccg aaacattgta
     7741 ttgaatccgc aacggcgtgc cttgcaggtt gaaggctttg cggaacgttt gggtcagata
     7801 gcgcgtatag ctgtcggaaa tcgcgtgcag cgaattgccg tgtaccacaa ttacgggagg
     7861 gttcatgccg ccttggtggg cataacgcat tttcggacgc accaagccgg cacgcggcgg
     7921 ttgctgacgc tcgatcgcgc tttgtaatac gcgcgtgatt ttcggcgtcg gcatcttaat
     7981 catcgccgcg ttgtaggcag cctgaatgct gtcaaacaaa ccgtctatac cgcgctcttt
     8041 caatgcggaa ataaagtgga acttggcaaa atcgaggaaa tacagtttgc ggttgatatc
     8101 gcgtttcact tgctcgcgac gctcttcgct gatgccgtcc catttgttga cggcaaccac
     8161 caaggcgcgg cctgcttcca aagcaaaacc ggcaatcgtc gcatcttggt cggcgatgtc
     8221 ctgctgcgcg tccaatacca aaacagcgac gtttgccgct tcaaccgcct gcatcgcttt
     8281 gataacggag aatttttcta ctgcttcatc cactttgccg cgacggcgca cgcctgcggt
     8341 atcgatgatg gtaaacggtt tgccttcgcg ttcgaaatcg atgtggatgc tgtcgcgtgt
     8401 cgtgcctgcc atatcgaaag caatcacgcg ctcttcgccg aggatggcgt taaccagtgt
     8461 ggatttgccg acgttcggac ggccgatgac ggcaaagacg ggatgttttg catcggcttc
     8521 ttcggcttcc ggctctggga aggtttccaa aatatcttca atcagataat acacgccatc
     8581 gccgtgcgca cctgaaataa cataagggtc gcccaaagca agttcgtaga actcggcggc
     8641 aagcacggcc ctgttgccgc cttcgccttt gtttaccgcc agataaacag ggcgcgggct
     8701 ttggcgcagg cggtcggcaa tgattttgtc ttgcggggtc aggccggtac ggccgtccac
     8761 caaaaacaca accgcatcag cttcatcgac agcctgcaag gtttgttttg ccatttcgtg
     8821 caaaatgccg ctgtccacaa ccggctcgaa acctccggta tcgatgacca aataaggttt
     8881 gctgccgact ttgccgtgtc cgtaatggcg gtcgcgggtc aggccgggca ggtcgtgtac
     8941 gagtgcgtct ttggtgcgcg tcaaacggtt gaataaagta gatttgccga cgttagggcg
     9001 gccgacaagc gcgatggtgg gtttcattat tgggtctttc tgtgtcaagt gccgttcggg
     9061 agaactgaac acgagcaggt gtccgttgga cacggtaaat aggttcttca cggcaagagg
     9121 ccataaagag ggagggtaaa atgccgtctg ataaataatg gacagcattt cagacggcat
     9181 ttatttcagc gaatcaagtt tcatttgaac caattcgcga ccgacagaat cttgaggcat
     9241 tttttctaaa gcctgtccgt agttttttaa ggcttcctgg ctttttccct gtgcggcata
     9301 gacatcgcct ttggtttcca tcagcagggg ggcgaagtcc gcttcaaccg gcgtgtcgag
     9361 cgcggcaagc gcggcatcgt attttttttg ttgcaacaac acaacgccca gacgctgcgc
     9421 cgccaacgcc tgaatcaggc tgtctttttg gttggacaac acccatttca aatggccttc
     9481 ggcaacatcg taacgctgcg cgtcaaattc ggttgctgcc gccatcagcg tggcttgggc
     9541 ggcggaaatg gaatgggggt agctttgttg gagcttggcc aattcggcat tgatttcgct
     9601 ttgcggggct ttgttttgcg ccttttctac gatgtttgcc agcaccgccg cagcttcctg
     9661 attttgggaa gccgcacggt tttggtaaac cgtgtatccc aagtagccga gcgccgccaa
     9721 aatcagcacg gcaaacagcc atttgcccgt ggttttccaa aaatatttaa agttgtctaa
     9781 ctcttgttgt tcttcgagat gggctgccat ttatgcgttc ttccattgtt gtaaagtagg
     9841 ggttaaatcc tcggcggcga cggtttgttg accgtgtgcg ccgttcatgt ctttgagcgt
     9901 aaccgtaccg ttcgccagtt cgtcttgcgc gacaatcagg gcaaagcgcg cgccgctgtt
     9961 gtcggctttt ttcatttgcg ctttcaggct ttgatagccg gaatgctgca ttacattgaa
    10021 atcttgcgcg cgcaaggctt gtgcgtattt catcacctgc aagtccgccc cttcgccttg
    10081 gtgcattgca tagacatcag gcgcggcgtt cacttccaga gagccgtatt cgctcaccaa
    10141 aagcagcagc cgttcgatgc ccattgcaaa gccgatagac ggcgcaggct taccgccaag
    10201 ttcctcaatc aaaccatcgt aacggccgcc gccgcacaca gtcgcctgcg cgccgagttt
    10261 gtcggttgtc cactcaaaaa ccgtctgatt gtaataatcc aaaccgcgaa ccaagcgcgg
    10321 attttcaata tattggatac ccaaaccgtc caacatcgcc ttgaagcgtg catagtggtt
    10381 ttgcgaagcc tcgcccaagt aatccaccaa acgcggcgcc gcgttgcaga tttcctgcaa
    10441 atcagggttt ttcgtatcca aaacgcgcaa aggattggtt ttcagacggc gtttgctgtc
    10501 ttcatccaat ttatcttcat aacgggtcag atattcaacc aatgccgcac ggtgtgccgc
    10561 gcgttcctca cggttgccca agctgttgat ttccaaagtc aggtaatcgc ggatacccaa
    10621 tttttcccac aagtcggcag acatcgcgat gatttccgca tcgatatccg gcccttcaaa
    10681 acccaaagcc tcgataccga cttgatggaa ctgacgataa cggccctttt gcggacgctc
    10741 gcggcggaac atcggcccca tataccacag cttttgcggg ctgttgtaca aaaggttgtg
    10801 ttcgaccacc gcacgcaggc aggaagccgt accttcagga cgcaagctca aactcaaaga
    10861 atcgtttgaa tcggagaagg tgtacatttc cttgccgacc acatcggttt cctcgccgat
    10921 ggagcggaca aacaaaccgg tttgctcgac aatcggcgta cggatttgcc gataaccgta
    10981 agcgcacgtc caacggttta ccgtatcttc aaacgcctgc caaaacgcag ccgtcagttt
    11041 gaaatctttt tgttcgacag gcagaaggtc gttcatgcct ttgacggatt ggattttttg
    11101 tgccatttca agtaagaatg cttaaatcaa attgcgggcg attatagcgg atttttaagg
    11161 gtttgtgagg ttgggggtgg tttgtggacg ggtaaaaatg ccgtctgaaa cagttttcag
    11221 acggcatttg acttactctg cacgtgcttg cctgatttgt tcgactgcaa actccgtctg
    11281 ccgcttggga tgatgtgtga aaaacaattt atttgaaatt gtctcggctt ttttcggcac
    11341 aaccgccaaa atcttacctg ccaaatttcc ctcacgggtt tgccaagcat ccaaaaactg
    11401 caccctgctc atcgaaacat gacccagcga cgggtcggca agcaaaaccg tattgccatc
    11461 tataccgcgc aataccgaga aatgatcatc cttgcggtat ttcagataca cgatgacggg
    11521 gattttcaac tgtgcaagct gctcgaaaga cagggcatag cctttcgctt caaaacccaa
    11581 atcaggcata atgcgccgca tatcctcaaa cgacgcgcgc atctgctcct tatccagctt
    11641 tttcaacact tcttcttccg tcagcgtttg cccgtaaaaa ttgttcaaaa gcgtcgccac
    11701 cgaagccgcc ccgcaggaaa aatccaaatc ctgctttaca atattgaaat cccgccgcgc
    11761 tttccagctt tgtattttga ctttgccgta ggctatgggg ttttcttccg caaagggaaa
    11821 agaatgtgca caaagcggca gggcggcggc gaggagcagg gaagcggcaa accgcctttt
    11881 ttgttccatt aaaaccctcc taaaaggcta cccggaattt ttaagcagcc ttttgattgg
    11941 taaacgttac gacggaagcc ttagcctccc gattgcagta tttcaattcc aagataaatg
    12001 ccgcatagaa aagataaaat acttaaatgg ttttagcggc ttaaatatct tatttaattt
    12061 tattgattgt tttcccaata gtttccgcaa taaataataa gaagtagaat aataaacaaa
    12121 cagaacatga aaataaaaat aaatctttca tcatctcagt aataaaattc caaagatttt
    12181 ccttatttcc atcaacaata aaaattatca gttcatgaat aaagaatact acaaagaaaa
    12241 tacttcctgc cgttagggat atttctaatc tgcttatttt cccttcgcta tatttacctg
    12301 ataaatagcc taaaaaagct acaatcaaat catccatttt aaaaatttct gtaaaaacag
    12361 ctaatgtcct taattcattt tgtccaagcc gtttgccatt gcgagggcaa tcatagacag
    12421 catcattacg gctgcggtga tttgtttttt cataacaaat tcttagggag gagataatgt
    12481 ttcatcaaag tcctcctgaa aggctgcctg aaatttccaa gcagcctttt tgtttgctta
    12541 ttaatcggat agtgtgacaa atataaggtg ggcggcaatc attgccgtaa cggcagccat
    12601 aactgtttta ttttcttcaa accaaagcga gttgagaaac atgaacatca agaaatatga
    12661 tggttcttca gttttgccgc tgctttcttg agttagaaga taaaatatag atatggcggt
    12721 tatgctgatt gctgttttta tatatttatt taccatatcg ttgggcaaca cccgtaacca
    12781 tacctgttgc caaagatcgg ttaaatcccc atacaacccc tgccggacct gctgtcgccc
    12841 ctccaaaagc tcccgcccct acggcaagag tgaatgctgt gggagaaaat ctgcttccgc
    12901 taagttgctg gttcatcata tatccgattc cgcctccgac tcctccgata aatgcaccta
    12961 ccggtcccca agccccctca gtctccttca tctccttttg agaaagctcc gccaactgca
    13021 tcggcgcatc tgcctgcgtg tggaacactt ggttttcaaa tgtctgattg tccaagccgt
    13081 ttgccattgc gggggcaatc atagacagca tcattacggc tgcggtgatt tgttttttca
    13141 taataactcc tttggattac aaggttggaa aatcaaaacc tgcttaaaat gtatgctgta
    13201 cgccaaattt cagttcggaa ctgctttgcc ctgaaacgtt gaaacgtgcg gatgcgttta
    13261 aagccgtggt tttggtgaaa ccgaaacctg cgccgaaatg ggcgtaggtg gatgtgtttc
    13321 ttgcggattc ttttttgccg tctatgcggt cgggctgcct gcccaaccat tggatgcctc
    13381 cggtcaggct gattctgtcg ttggcggcaa atgagatgtt ggggttgagc agcaggtagt
    13441 tgcccgattt gtagcggatg ccgtctgaaa ggattttgct gccgttgata cggtaggcgg
    13501 cggttaggga gaggacgacg gggtcgatag ctttgtaggt ggtcgcgcca atcagccatg
    13561 atttgcctga cgaggctttg ccgcgtgatt tttcgtaaag tgtgccttcg agaaagccaa
    13621 taagggcggg atttttgtcg tctttgagga aggtatggct gataccggcg gatatgtcag
    13681 acatccgttt gttgcgggtt ttgccgttgc cgtcgagttt gcgttcttcg tgccacagat
    13741 agctgccgct gccgtaaatg tcggtattgc cggtcagtcc gtagcgcaaa ccgagcgtgc
    13801 cggcgagcat atcggtattg ctgccgtttt cttggatttc ggtcggaatg gggataaacg
    13861 aggttgcgcc ggtttgaatg taaaccggtg cggcaagtgc ggcgcggctg ttttcgctat
    13921 tcaggtaggt aagggaagtt tccagtttcc atttgccctt gtcggttatt atgtcttcaa
    13981 tcgtcaaggg caggtcggca taagcggata aaggcaggat ggcgggcaag gcggacaaaa
    14041 agatacgttt catatttcct attattgata ttaattctta tttaagaata gtaatgaata
    14101 tccgataatt ctgtctaaat aaatttttaa atcaaaaaaa acgatatatt tgtttttatc
    14161 aattatgttt tgatatgccg tctgaaaagt ttgtgaaaaa cggttacaat ccgccgcatg
    14221 aaaaaacgca ataatcctct tccgttgttg aacggtgtca aacccagtta tttggtgctg
    14281 ccgcatgaaa agcagtttta cgggctgccg ctgctgcatt ttctgtgcat ccgctttcct
    14341 tttgtgggcg cggatgattg gcgcaggcgg ttgaacagcg gttttgtggt cggttcggat
    14401 ggtgcggcgt tggacgaaca tactttgttc gagccgggta aggtgatgtt ttattatcgg
    14461 gaaaccagca gtgaggatga gccgcgtatt ccgtttgaag aaaagatttt gcatattgat
    14521 gaacatttga ttgtggtgga caagccgcat tttctgcccg tcatccccag cggcaggttt
    14581 ttgcgggaaa ccctgctcac gcgcctgcgc ctgcgccccg aattgcagca tttgaatgtt
    14641 gaagacatta cgccgctgca ccgcttggac aaggatacgg caggcgtgat gctgctgtcg
    14701 cacaatcctg ccacgcgcgg agcctatcag acgatgtttc aaaacaaaac ggtatggaaa
    14761 acgtatgagg cgcttgcgcc gacaaggacg gatttgccgt atccgctcga tgtggtttcg
    14821 cgtttggtaa ggggtgagaa attttttacg acgcaagagg cggaaggcga accaaacgca
    14881 catacgacgg tcgaactgat tgaaaacagg ggggaattca gcctttaccg ccttacgccg
    14941 cataccggca agaaacacca gttgcgcgtg catatgatgg gtttgggtat gccgctgttg
    15001 aatgacgcgc tctatcccgt gccgtctgaa gcgggcagcg aggattatcg gaaacctttg
    15061 aagctattgg caaaaaagat tgcgtttgca gatcctttgt cgggtcggga aagggtgttt
    15121 tgcagcggat tttgcctata atgagaggga acgcaaaccc gtatcggcgg atgccgccga
    15181 tacgggtttt acattatcga tgccgtctga aaggggcttt atccgttcag gcatacgggc
    15241 gcgaagcgga ggatggtgtc gcgcaagacg atgagtttgc cgtgaaagaa atgcgtggaa
    15301 ccggcgatgg taatgacggg caaatcttgc ggttccgccc atttcagtgc tttcccgatt
    15361 tcgacgactt cgtcttccgc gccgtgtatc atcagcgttt tggcaacatt gggaacggcg
    15421 gacggctcgg ggcggccggt atagtggcag actgccgcgc cgatgagcag caataaatcg
    15481 ggtatgcgtt cttgtgcggc aaatgtggcg acataaccgc cgaaggagaa gccggataag
    15541 gcaaattcgg gggcttcggg atgttgggca cgggcatagt cgataacgga gaggcagtct
    15601 tgcgtttcgc cgcgtccgta atcgtgtgtg ccttcgctgc cgcctacgcc gcgaaggttg
    15661 ggcaggtagc agtggaagcc gagtttgctt aaggctttgg cggcagtttg gataactttg
    15721 ttggtgtttg ttccgccctg gagggggttg ggatgattga tgacggcaac accgcgtgcc
    15781 ggtacttgtt cggacgggat atggatggtt tctaaaatgc cggcaggacc ggatatgtgt
    15841 atggtttcgg gtttcagcat aatgatttcc tcttgatttt ccgcatgttc cgatgcagcc
    15901 gtgaagatag ttgcttaaag gtggaaatgc ttgccgttgt tggggttgga cgctttcagg
    15961 ccggcaagca tacggtggaa gtccaatccg tatgcgttgc tcagtttgtc ggcacgtttt
    16021 ttgagtttgt ctatgttttg ctctttgggg ctgctttgga aggccattac cgcgacattg
    16081 ccgtggcttt cggcaggaag ttccaatacg cgcccttcaa aaacgctcaa caaccgttcg
    16141 atgaagcgtt gatagcgttt gtcgccactc caccagttgg taacgaatat gccgtctgaa
    16201 gagagtgcgt tgcggcagtc tcggaagaac ggttcttcaa ccagcgcatc gatgatttgt
    16261 tcgccgtcga acccgtccac caaaatcaca tcggtgttgt ggcggaagac tttgatatat
    16321 tctgcaccgt ctgcttcaat aatttcaaat ttctcgccct cgaaaggcaa ctcgaacagg
    16381 ctgcgggcaa tggcgatgac ctgcgggttg atgtccacgg cggtttggcg cgtgtcgggc
    16441 aggtaggtgt ctatccagcg tgcaaacgaa ccgccgccca agccgatttg ggtgatatgc
    16501 tgcggaagag cgtcggtaaa cagcagccag cccatcatgg cacggctgta agagagtacc
    16561 agctcggacg ggtggtcgag gttcattgag ctttggatgg tgtcgctgcc caagtgaagc
    16621 gaacggatat tgccttcttc ggaaatgccg acttcgggaa agctggattt tgcaggacgc
    16681 aggcggcggt agggatgtct tgccatcggt gtgaacggtc ggtttgaaag gcagatattt
    16741 tatcagaatg ccgatggttg tcctgcttca aattaatttc ttttcaaata aattacttat
    16801 tcggatttgc cggggctttc ggataaattc cttgccaagg tgcggcattg cctgcataat
    16861 tcgctttctt tgccggtata gctcagttgg tagagcacct gacttgtaat cagggggtcc
    16921 cgagttcgac tcttggtgcc ggcaccagat ttgacagccc gattgtgtaa aacagtcggg
    16981 ctgttttgca tttgcggcac ggtcggtggc agggctgcgg gcatttcact ataatagccc
    17041 gtttcaaact gaaaaggccg tctgaaaagg gcggggtaac aatatctgat gattactgtg
    17101 aacacactgc aaaaaatgaa ggcggcgggc gagaaaatcg ctatgctgac cgcttacgga
    17161 tccagttttg ccgcgctgat ggacgatgcc ggcgtggaaa tgctgctggt cggcgactct
    17221 ttgggtatgg cggttcaagg gcggcagtcg acgctgcccg tcagcctgcg cgatatgtgc
    17281 tatcacaccg aatgtgtagc acgcggtgca aaaaatgcga tgattgtcag tgatttgccg
    17341 tttggtgcat atcagcagag taaggagcag gcgtttgccg ccgccgccga actgatggct
    17401 gccggcgcgc atatggttaa acttgaaggc ggtgtgtgga tggcggaaac taccgaattc
    17461 ttgcaaatgc gcggtattcc ggtttgtgcg catatcggtc tgacgccgca atccgtgttt
    17521 gcctttggcg gatataaggt tcagggtcgc ggaagtaagg cgcaggcgtt gcttaacgat
    17581 gccaaggcgc atgacgatgc gggggcggcg gtcgtgttga tggagtgtgt tcctgcggaa
    17641 ctggcaaaaa aggtaactga atccgtttct tgtccgacca tcggtatcgg tgcgggtgta
    17701 gattgcgacg ggcaggtttt ggtaatgcac gatatgctcg gtatttttcc gggtaagacg
    17761 gcgaaattcg tcaaaaactt catgcagggg cataatagcg ttcaagcggc ggttcgtgcg
    17821 tatgtcgaag aagttaaagc aaaaaccttt cctgctgcag aacatatctt tacagactga
    17881 acggtttaca tgcagcatgc cgtctgaaag ccgtttcaga cggcattttg ttttgccgtg
    17941 cgcggcgcgt ataatcggcg cgttttgtcg ggcaggaagc ccgaaggata aggattaccg
    18001 ttatgcaaat catacatacc attcgagaac tgcgcgcgtg gcgtaaaaat gcgggaaagg
    18061 tggcatttgt gccgaccatg ggcaatctgc atgaagggca tcttgctctc gttcgcgaag
    18121 ccaaaaaacg cgccgacaac gttgttgtca gcatattcgt caaccgcctg caattcggtc
    18181 agggcgagga tttcgacaaa tatccgcgta ctttgcaaca ggatgcggac aagctggaag
    18241 ccgagggtgt tgccgttgtt ttcgcgcccg atgagaaaga actctatccg aacgtggaac
    18301 agcgttacaa cgtcgaaccg cccaatctgc aaaatgagtt gtgcggcaag tttcgtccgg
    18361 gacatttccg cggggtggcg acggtggtgt ccaagctgtt caatattgtc gcgccggatg
    18421 ttgcctgttt cggcaaaaaa gactatcagc agcttgccgt gattaaaggt ttgaccgagg
    18481 atttgaattt tgatgttgaa atagtgcctg tcgatacggg gcgcgcggaa gacggtttgg
    18541 cactctccag ccgcaaccag tatttgagtg cggcggaacg cgacgaagca ccgcgcctgt
    18601 cccgcgaatt aaaggctgtt gccgaatcct tggcgcaggg cagtttggat tatgcaggtt
    18661 tggagcggca ggctgccgac catttgaccg ctgcgggctg ggtggtcgat tatgtcgaaa
    18721 tccgccgcgc cgatacgctc gaagtggcgc gggcgggaga taagaaactg gtggtcttgg
    18781 ccgccgcccg tctggggacg acgcgcctga ttgacaattt ggaaataaaa ctcccttaaa
    18841 ccgcaagcgt cgggaatgcc gtctgaagcg gatttgcgtt tcagacggca tttatttttt
    18901 tgaacggggt ttcgcaaatc tacaaacgat tctgcttgtg ataaagttac gcctgattat
    18961 atgccgtctg aaggttcgga cggctgtcgg ataaaggaag attatgttac ccgcccgttt
    19021 caccatttta tctgtgctcg cggcagccct gcttgccggg caggcgtatg ccgccggcgc
    19081 ggcggatgcg aagccgccga aggaagtcgg aaaggttttc agaaagcagc agcgttacag
    19141 cgaggaagaa atcaaaaacg aacgcgcacg gcttgcggca gtgggcgagc gggttaatca
    19201 gatatttacg ttgctgggag gggaaaccgc cttgcaaaag gggcaggcgg gaacggctct
    19261 ggcaacctat atgctgatgt tggaacgcac aaaatccccc gaagtcgccg aacgcgcctt
    19321 ggaaatggcc gtgtcgctga acgcgtttga acaggcggaa atgatttatc agaaatggcg
    19381 gcagattgag cctataccgg gtaaggcgca aaaacgggcg gggtggctgc ggaacgtgct
    19441 gagggaaaga ggaaatcagc atctagacgg actggaagaa gtgctggctc aggcggacga
    19501 aggacagaac cgcagggtgt ttttattgtt ggcacaagcc gccgtgcaac aggacgggtt
    19561 ggcgcaaaaa gcatcgaaag cggttcgccg cgcggcgttg agatatgaac atctgcccga
    19621 agcggcggtt gccgatgtgg tgttcagcgt acagggacgc gaaaaggaaa aggcaatcgg
    19681 agctttgcag cgtttggcga agctcgatac ggaaatattg ccccccactt taatgacgtt
    19741 gcgtctgact gcacgcaaat atcccgaaat actcgacggc tttttcgagc agacagacac
    19801 ccaaaacctt tcggccgtct ggcaggaaat ggaaattatg aatctggttt ccctgcacag
    19861 gctggatgat gcctatgcgc gtttgaacgt gctgttggaa cgcaatccga atgcagacct
    19921 gtatattcag gcagcgatat tggcggcaaa ccgaaaagaa ggtgcttccg ttatcgacgg
    19981 ctacgccgaa aaggcatacg gcagggggac gggggaacag cggggcaggg cggcaatgac
    20041 ggcggcgatg atatatgccg accgaaggga ttacaccaaa gtcaggcagt ggttgaaaaa
    20101 agtgtccgct ccggaatacc tgttcgacaa aggtgtgctg gcggctgcgg cggctgtcga
    20161 gttggacggc ggcagggcgg ctttgcggca gatcggcagg gtgcggaaac ttcccgaaca
    20221 gcaggggcgg tattttacgg cagacaattt gtccaaaata cagatgttcg ccctgtcgaa
    20281 gctgcccgac aaacgggagg ctttgagggg gttggacaag attatcgaaa aaccgcctgc
    20341 cggcagtaat acagagttac aggcagaggc attggtacag cggtcagttg tttacgatcg
    20401 gcttggcaag cggaaaaaaa tgatttcaga tcttgaaagg gcgttcaggc ttgcacccga
    20461 taacgctcag attatgaata atctgggcta cagcctgctt tccgattcca aacgtttgga
    20521 cgaaggcttc gccctgcttc agacggcata ccaaatcaac ccggacgata ccgctgtcaa
    20581 cgacagcata ggctgggcgt attacctgaa aggcgacgcg gaaagcgcgc tgccgtatct
    20641 gcggtattcg tttgaaaacg accccgagcc cgaagttgcc gcccatttgg gcgaagtgtt
    20701 gtgggcattg ggcgaacgcg atcaggcggt tgacgtatgg acgcaggcgg cacaccttac
    20761 gggagacaag aaaatatggc gggaaacgct caaacgtcac ggcatcgcat tgccccaacc
    20821 ttcccgcaaa ccccggaaat aatgcaggtc catcctttca gacggcataa ggtttgccgg
    20881 gaagccgggg cattcgggca aacggcacgc agttcgcacg cgttttgcac ggcacgccga
    20941 acccatcggc cggcaggatg gcatccgtta aggaaattct gatgaaacac accgtatccg
    21001 catcggtcat cctgcttttg accgcttgcg cgcaattacc tcaaaataac gaaaacctgt
    21061 ggcagccgtc cgaacacacc cgcagtttca cggcggaagg gcggttggca gtgaaagcgg
    21121 aagggaaagg ttcgtatgca aatttcgatt ggacatacca accgcccgtg gaaaccatca
    21181 atatcaatac ccctttgggc agtacgctcg ggcagttgtg tcaagacagg gacggcgcat
    21241 tggcagtgga cggcaaagga aatgtctatc aggcggaaag tgcggaagaa ttgagcaggc
    21301 agctggtcgg tttcaaactg ccaatccaat atctgcatat ctgggcagat ggcaggcgtg
    21361 tggcgggcgc gccttaccgc atcctgccgg acggcatatt ggaacaatac ggttggactg
    21421 tcggcagaac cgccgacagt ggggggcaag tccgaacgtt gcaactgaat aacggaaatt
    21481 tgaacatcag gctggttttc accgaaatcg gtatgccgtc tgaaaccgaa accccggaac
    21541 gctgtgcggc gcgcacgaga taaggcggac agatgaatat tgcggacgga cggcaggcgt
    21601 ttcccgcacc tgcaaaactg aatctcgatt tgaggattac tggaaagcgg gaagacggtt
    21661 atcacaatat cgaaagcata ttctgcctga tagatttgca ggataccgta tatttgaaac
    21721 cgagggacga cggcaaaatc atcctgcaca atcctgtcgg cggaataccg caggaagccg
    21781 atttgagcta ccgtgccgca tcgttgctgc aaaaatatgc gcgcaacccc gccggcgtgg
    21841 aaatatggct ggacaaaaaa atcccgacag gggcgggttt gggcggcgga agctcggatg
    21901 cggcaaccgt tttgctggtg ttgaaccgtt ggtggcagtg cggtctgacg cagcggcagc
    21961 tcattgattc gggcgcggct ctgggggcgg acgtaccgtt ttttattttc ggcaaaaatg
    22021 cgtttgcgcg gggtataggc gacaggctgg acgaaatgga tattccgaaa cagtggtatg
    22081 tcatcgtcaa accgcccgtc cacgtttcca ctgcaaaaat tttcacacac gaaggcttga
    22141 cacgaaattc cgcctcaagc ataatgccga ctttccaaaa cctgcaaccg tttagaaatg
    22201 acatgcaggc agttgtattt aaagaatacc ctgaagtttg gaaagcctat tccgaattgt
    22261 cccgatatgg atttgcctta atgacaggtt ctggtgcgtg tgtattcacg gcgtgtcaag
    22321 ataggaatag cgcatacaat atataccgac aagtttcaga tttgtacgag gcatatttgg
    22381 cagagggtct ttcaaaacat ccttggttgt ccgtataaac att
//
LOCUS       DAXIEB010000019        22376 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000019 DAXIEB010000000
VERSION     DAXIEB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.019"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            1..99
                     /locus_tag="ACFOA3_000648"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFOA3_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFOA3_000649"
     CDS             complement(120..1034)
                     /locus_tag="ACFOA3_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6897695.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAVKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFOA3_000650"
     CDS             1290..2759
                     /locus_tag="ACFOA3_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6897696.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVGAAFATPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFOA3_000651"
     CDS             3011..3382
                     /locus_tag="ACFOA3_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897697.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFOA3_000652"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFOA3_000652"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6897698.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFOA3_000653"
     CDS             4910..5704
                     /locus_tag="ACFOA3_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897699.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAITTESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5892..6668
                     /locus_tag="ACFOA3_000654"
     CDS             5892..6668
                     /locus_tag="ACFOA3_000654"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6897700.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="ACFOA3_000655"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="ACFOA3_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7429..9900)
                     /locus_tag="ACFOA3_000656"
     CDS             complement(7429..9900)
                     /locus_tag="ACFOA3_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6897701.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPIDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILKHRVSDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFOA3_000657"
     CDS             complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFOA3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6897702.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSTARLNGFQTAL
                     AEAVELVKAAGGNPVLVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11080..12360)
                     /locus_tag="ACFOA3_000658"
     CDS             complement(11080..12360)
                     /locus_tag="ACFOA3_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6897703.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12362..12490
                     /locus_tag="ACFOA3_000659"
     CDS             12362..12490
                     /locus_tag="ACFOA3_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897704.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12513..13238)
                     /locus_tag="ACFOA3_000660"
     CDS             complement(12513..13238)
                     /locus_tag="ACFOA3_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6897705.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMNQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFOA3_000661"
     CDS             complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFOA3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6897706.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGTGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVKELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFOA3_000662"
     CDS             complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFOA3_000662"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6897707.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDALGMPRSEKDCGVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15720..17129
                     /gene="leuC"
                     /locus_tag="ACFOA3_000663"
     CDS             15720..17129
                     /gene="leuC"
                     /locus_tag="ACFOA3_000663"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6897708.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17226..17480
                     /locus_tag="ACFOA3_000664"
     CDS             17226..17480
                     /locus_tag="ACFOA3_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897709.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17542..18183
                     /gene="leuD"
                     /locus_tag="ACFOA3_000665"
     CDS             17542..18183
                     /gene="leuD"
                     /locus_tag="ACFOA3_000665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6897710.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18358..19086
                     /gene="dcm"
                     /locus_tag="ACFOA3_000666"
     CDS             18358..19086
                     /gene="dcm"
                     /locus_tag="ACFOA3_000666"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6897711.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKIS
                     PVRTVRKISKRQTGREKQYSQLGY"
     gene            19201..19629
                     /locus_tag="ACFOA3_000667"
     CDS             19201..19629
                     /locus_tag="ACFOA3_000667"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6897712.1"
                     /translation="MTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKD
                     ESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGLRL
                     FGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19645..20376
                     /locus_tag="ACFOA3_000668"
                     /pseudo
     CDS             19645..20376
                     /locus_tag="ACFOA3_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20410..21480
                     /gene="leuB"
                     /locus_tag="ACFOA3_000669"
     CDS             20410..21480
                     /gene="leuB"
                     /locus_tag="ACFOA3_000669"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6897713.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIEQGLDVSYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLNRPLRPERGLLAIRKDLNLFA
                     NLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGENKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTND
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21643..22206
                     /locus_tag="ACFOA3_000670"
     CDS             21643..22206
                     /locus_tag="ACFOA3_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6897714.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMEKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5422 a         5883 c         5790 g         5281 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg tggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt taccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 aattttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 gggcggcgtt tgccacacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ctgcctgtcc gccgcctgat tgttgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ctatactgct ggacgaactg gcggcaaaac aggaggcagc
     4981 catcgctccc cacctgcttg ccgacggaac caaggtatgg gtacgcaaag caggcaggca
     5041 caatgcgcgg tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt acaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg caggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacggaa aaaacatcag
     5401 ctttctagat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtact gtctgagcag cctgccgaaa tacaaaggct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atataccgcc
     5701 ataagccgcc gcctgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctagt tgccggcgac ctgaatccag gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgt gcgtttgtag
     6661 atcaataagc tgtttgaaaa tcccgtctga acggcaccag caatcaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaatcaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat acacatttcc gtcattcacg cgcaggcggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatggatt
     6961 cccccctacg cgagactgac gacggtgttc tgcagtagcc aaagcagaaa accatcctta
     7021 ttgtcatttc atcatgatat aacaataagg atgttaaaaa ttagttatca ttatatgcat
     7081 gatatataag aaaaattgtc ccaataagaa atgttatgca gaaaataaaa tgaaaaggat
     7141 ggttttcatt gaaaatggtt gttctgccac cgtcatacac agaaccggta tttggaaaaa
     7201 aaataatcca tattgacatc attattccat ttatcgcata aatccacttt ttaatttttt
     7261 tcctcattaa tacaatctct tatttaataa aatcaaatac ctagcaattc gatctcaaat
     7321 tttgaacgcc aacgtgtttg agcggtaaca atacatactt taagtttgca attcttcatc
     7381 gtcttatttt tgttttcatc aactatacgt ctaaagcaac gccgtccgtc attgttcgga
     7441 cggcattttt tcagactgca ttttcccccg tttgtgaagg cgcagggcgt tgcccaaaac
     7501 cgccagcgaa ctgaagctca tacccagtgc ggctatccac ggttggacat agcccaaaac
     7561 ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt gccggataat
     7621 atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat cttcgttcaa
     7681 taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg cggcggatac
     7741 gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca gcactttttt
     7801 cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg cttgggcgcg
     7861 gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc ggtcgccgct
     7921 gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg ccgcgctgtc
     7981 tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc cgaggtaaac
     8041 cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga cataggatgc
     8101 cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc ccacgccttc
     8161 gccgatgcgg ttgaggcgtt gtttaatagc aatgtcgggg acgctgccgt ctgaaacacg
     8221 gtgtttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag cctgcgccac
     8281 cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga cggcgggatt
     8341 gccttgggtc agcgtgcctg ttttgtcgaa aatgatgtcg gtggtttggg cgagggtttc
     8401 gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc agcgtaccgg tagaagctgc
     8461 cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca gcagggcgac
     8521 ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga agacggggac
     8581 ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca actcggcagt
     8641 gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca gtcgcgtgcc
     8701 gccgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg tacctgcggt
     8761 tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca tagattcgtc
     8821 aaccgtgctt gcgccttcca gcaccgtgcc gtcaatgggg atggtttcgc ccggtttgac
     8881 cagcacgata tcgcccgcct tcaacttgac gacagctgcc tcgcaggttt cctgcgtatc
     8941 ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc tctcggcggc
     9001 atcgcctgcc ttacggcggg caatgtgttc cataaagcgt ccgcccagca ggaaaaacag
     9061 cagcatggct atggattcga aatacatccc ctgccccgca ttggtcgcaa ggctgtaaac
     9121 gccggcgata aaggtcatga tgatggcgac ggcaatcggc gtatccatgc cggcgcggcg
     9181 gtttttcagg tcgcgcaacg cgccttgata aaacgggacg gcgcaataga atacgacggg
     9241 cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt cgatgtcgcc
     9301 gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca gcccggcaac
     9361 ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga ttttttgcgc
     9421 gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt cggaaaggcg
     9481 gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga ggtcgatgcg
     9541 gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg cggcgcaggt
     9601 gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca caaagtcgga
     9661 ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg gcggcagctc
     9721 ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg cgtcaataat
     9781 gctttgtgcg accgcctgac agccggcgca gcaggtttcg cggtcttcgt tttcgtaacg
     9841 gacagtcagg tggaggtgtt cgggaacatc cagaccgcag tggaaacagg tttttttcat
     9901 ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag actcggcaat ggcttcgcgc
     9961 agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat ttttcccgca
    10021 gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt tttgttgtac
    10081 accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc cacgtcttca
    10141 atctgctgtc cgctgttcgg agcggcggca tcgacgacgt gcagcagcac atcggcttgc
    10201 gcggtttctt ccagcgtggc ggaaaaggcg gaaatcagtt tgtgcggcag atcgctgacg
    10261 aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag ccgccgcgcc
    10321 gtcgtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt ggtcagccgg
    10381 ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt tttgattgtg
    10441 cccgattcgc gggacttgcg gcgcagggcg cgctgttttt tgaggttggc aagctgtttt
    10501 ttcaaggcgt tgatccgatg ggcgatcaat cggcggtcgg tttccagttt ggtttcgccc
    10561 ggccctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc gcgtatcaag
    10621 cgtcccgcca aatggctcaa ttgtgccaac tcaacttgca gcctgccttc ctgcgtgcgg
    10681 gcgcggcggg cgaaaatcgc caaaatcaac cctaccctgt ccagcacgcg gcatttcagt
    10741 tctttttcaa ggttgcgttc ctgcgtgggc gtaagttcat ggttgaatac gaccaaatcg
    10801 atgccgtctg cggcaactgc ttccgacagc tccgccgcct tgcccgtgcc gacaaacagc
    10861 gcggtgtgcg gacggtcgcg tttggcggtt tccacaagga cgggattgcc gcccgccgcc
    10921 ttgaccagct cgacggcttc cgccaatgcc gtctgaaagc cgttcagacg ggcggtactg
    10981 ctgcccgtac cgtctttgtc caacattacg cccaccagca tgacgcgttc gggttgcgct
    11041 tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt caatcctgta aataaagggt
    11101 tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg cggctgcccg ccattccgtc
    11161 aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc tgccgggtcg
    11221 ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc agtggatgaa
    11281 atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa aataagggta
    11341 atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt tgcggacata
    11401 gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc ttgagcggga aaagcgcgaa
    11461 ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc tgacggggcg
    11521 gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc ctgccgcctc
    11581 ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg ggtctggacg
    11641 ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga taggctgtat agagcagctc
    11701 ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg cgttgcggta
    11761 atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct caaacctgcc
    11821 gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg ccgctcctgc
    11881 ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca ttaatccgtc
    11941 cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt cctcttccgt
    12001 gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc gttcggtcag
    12061 cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt cttcgggatt
    12121 gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg cgtgtgccag
    12181 cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat cgccgccgtc
    12241 cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt ggttttgggt
    12301 atgatagaaa acgccggccg ccgaacccaa caggagcagc cgaataaaaa gggatttcat
    12361 aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac ttgccaatgc
    12421 ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg ccgaacccga
    12481 tgccgtctga actttcagac ggcatcgggt gtttatttgt ccacccggat gggtgcaggt
    12541 tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg gatgcgtccc
    12601 tgcgatttgc ggtgggcatt ggaatccctc agggaatagg cgcaaccgca atattcctgc
    12661 tggtagaagt tttcgcgttt gctgatttca atcatgcgcg cgctgccgcc gcctttgcgc
    12721 cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcgcggcg gccacagtcg
    12781 ttaatctggt tcatgttttt ccaacgggaa atgcccagcg agctggtgaa gacggggaac
    12841 ccatgttcat gcgcgtattg cgcggctttt tcaaaacgca tatcgaaaca catcgtacag
    12901 cggatgccgc gttccggctc aaactccata cctttggctt tggcaaacca ttctttgcgg
    12961 tcgttttcgt aatcgtcgtc tttgtcgatg aaggggatgc cgaacttttc cgcaaagcgc
    13021 acgttttcct cttttctgag catatactct ttgtgcggat ggatattggg attgtaaaaa
    13081 taaatggtgt agtcgatgcc ggaggcaagc atcgcctcca tcacttcgcc gctgcacggg
    13141 gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag tatggggcgg
    13201 tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat atctgtcgaa
    13261 tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacagggtg tcagggcatc
    13321 agcccgagct gcctgaacga acggacggtt tgcgccgtta cgataaaatg gtcgagcagc
    13381 gacacatcga ccagcgacat tgcctgtgcc agccgccttg tgaacatgat gtcttcctgc
    13441 gaaggttcgg gcgagccgcc cggatggttg tgcgcgataa tcaggctgtc ggcatactcg
    13501 tccaaagcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac cgtaccgcgc
    13561 gacagctctt tgaccgcaat cagttggttt tggcggttca gcagcagcgc gacgctgact
    13621 tcgacttttt cctgccccaa atgaaagcgt aaataatcgg ccaccgtatc cggatcggac
    13681 aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc cctgaccacg
    13741 gcaaactgtg tgaaacttgc cgtccccgtc cctttgtatg ccgacagttt gccgacctcc
    13801 gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc caaatcgacc
    13861 gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc atcactcaac
    13921 gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg ccattgcttg
    13981 atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag atgttcccac
    14041 acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc atctgtattt
    14101 caaaacagtc ttacgccgtt tcttccaact cgatggacgc cgccagaagc gacaggcgcg
    14161 ccattacgcc gtatacatag aggcggttgt gttcgttgtc cacgccgcag tctttttcag
    14221 aacgcggcat acccagcgca tcccattgct cgaatacgcg cttgcaggct tcgggagctt
    14281 cttgggagtt gtcgccgttg ccggtgggaa tactgttgga cagcggaaca aacaccatac
    14341 cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa aagccgccga
    14401 tgacgaagcg gtccatcata tagacgacgg gttcgcacac cgcgccgttt aaggtttcat
    14461 aagtataaat cccttcttgg acaatcactt cgctgacttc caagccttct ttgactttcg
    14521 ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat ttgacgctca
    14581 tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt ttgtcggtaa
    14641 tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc gcttccgcca
    14701 acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca aaataagggt
    14761 taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact tggttgtacg
    14821 cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc ggcaaaacgg
    14881 tttggctgat gcctttgagg atgtcgggaa tgccggcgga caaatcgttg ttcaacaaaa
    14941 ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc aataaaggtt
    15001 ccaacaggat tttgtcgccc aatgcggttt caaattcggt cggttcggtt acttccggat
    15061 tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg agggcgtaaa
    15121 cgttttgcag gtaaaacgta ttgcgcgtgt ggttttcagg aataatcaat acggatttcg
    15181 ccgtttcaca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc gggataaagt
    15241 tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac gaaattttgt
    15301 aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgcgcg ttccactgcg
    15361 tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca aacgcctgaa
    15421 gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg cctctgtgtg
    15481 ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt attctttcgt
    15541 atcgaatcat gctttagata taactgttta ctgaaatgga caatatgtct aattttttcg
    15601 ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt tgcgcggtat
    15661 aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag aacaacatca
    15721 tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc gaagaagaag
    15781 acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc agccctcagg
    15841 catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc gtcgtctcca
    15901 ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac ccgatttcca
    15961 agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc gcctattttc
    16021 cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa ggcgcgaccc
    16081 tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc gcattcggcg
    16141 cactggcgca cggcatcggc acttccgaag tcgagcacac catggcgacc caatgtatta
    16201 ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa gcgggcgtta
    16261 ccgccaaaga cgtggcgctc tacatcatcg ggcaaatcgg cacggcaggc ggtacaggct
    16321 acgccatcga gtttggcggc gaagccatcc gcagcctttc tatggaaggc cgcatgactt
    16381 tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc gaccaaacca
    16441 ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg gacaaagccg
    16501 tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa gaataccgtt
    16561 tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa atggttttag
    16621 acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa cgcagcggta
    16681 tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac gaaatccccg
    16741 tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg cgcgaagccg
    16801 ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgttg atcgtccccg
    16861 gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt ttcatcgaag
    16921 ccggttttga atggcgcgaa ccgggctgct ctatgtgcct cgccatgaat gccgaccgcc
    16981 tgaccccggg tcaacgttgt gcctctactt ccaaccgtaa ctttgaaggc cgtcaaggca
    17041 acggcggacg tacccacctc gtcagccctg ccatggcagc cgcagccgca gtgaccggtc
    17101 gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa gaatttgacg
    17161 cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa ccaaggagtt
    17221 taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc gcctgcgccg
    17281 gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca caggccgccg
    17341 ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac aaaaccaaag
    17401 aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc ggcggaaaaa
    17461 tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc agacggcatc
    17521 gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat cgtcgccccg
    17581 ctcgaccgca gcaacgtcga taccgatgcc atcatcccca aacaattttt aaaatccatc
    17641 aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga ccacggcgaa
    17701 ccgggtatgg acaacagcaa acgtccgctg aaccccgatt tttccctgaa ccaaccgcgc
    17761 tatcaaggcg cgcaaatcct gttgacgcgc aaaaacttcg gctgcggctc ttcacgcgaa
    17821 cacgcccctt gggcattgga cgactacggc ttccgcgccg ttatcgcccc cagctttgcc
    17881 gacatcttct tcaacaactg ctacaaaaac ggccttttgc ctatcgtttt gacagaagaa
    17941 caagtcgatc ggcttttcaa agaagttgaa gccaacgaag gctaccggct ctccatcgac
    18001 cttgccgaac aaaccctgac cacccctagc ggcgaaacat tcacattcga cattaccgaa
    18061 caccgcaaac actgtctctt aaacggcttg gatgaaatcg gactgaccct gcaacatgcc
    18121 gacgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct gtttaacggt
    18181 taaaacctat gccgtctgaa ggccgttcag acggcattcc tgataacata taattgttgc
    18241 ggctaatgta aacagagggg cgataaattg ctttcatctt ttaagcgggc ttagcgacac
    18301 tctaaattaa tgcaataatg caactctcca tttcaaataa atttttagat ttttaatatg
    18361 caacaaatta aatttattga cttattttcc ggaatgagtg gcatcaggaa aggattcgaa
    18421 caagcctgtc ggaaacagtc ggttgcttgc gaatgtgttt ttacttcaga aatcaaaccg
    18481 gcagctttgg aagtattgaa gcaaaactac cctgatgaag tgccgtatgg agatataacg
    18541 aaaattgaaa caggggatat tcccgatttt gacatcctgt tggcaggctt cccttgtcag
    18601 gctttttctt tcgccggaaa aagattgggc tttgaagata cacggggaac gcttttcttt
    18661 gatgtggcaa ggattttaaa agcaaaaaaa ccaaaaggtt ttattttgga aaatgtggaa
    18721 ggattggtga cacacgatag aaaagatccg acacaaaaaa tagggcgcac cctgaccgtt
    18781 attttggaaa ccttggaagc attgggctac tatgtttctt ggaaagtttt aaatgcaaaa
    18841 gatttcggca ttccccaaaa caggaagcgc atttatctga caggcagttt gaaatccaaa
    18901 cccgacttat ctttcgaaac aagcccaagt ccgaaattaa aaaatatttt ggaatcggga
    18961 ctgcctactg aaagcagccc tttcatcaag aaattgctaa aaaaaatttc ccccgtccga
    19021 actgtacgga aaatcagtaa aagacaaacg gggagggaaa aacaatattc acagttggga
    19081 tattgaatta aaaggcgcag taaccgaaga agagaagcaa ttgttaaata tccttctaaa
    19141 agaacggagg aaaaaaaatg ggcttcagaa atcggcatag attggatgga tgggatgcct
    19201 ttgacaaaag cgcaaatttc aactttctat aaacaccccg atctccaaaa tattttggac
    19261 agcctgacag ataaaggcta tttggtttta gagcatccga aacaaaaaat tggcgggcaa
    19321 agaatcaaag atgaatccct gcccaaaggc tacaatattg tttcaggcaa aaaatctttt
    19381 gaaatcaata aaatattaga tccaaacgat gttgcgccaa ccttggttgc aatgaatatg
    19441 gagcaccttt tcgtcgttga caacggcggt ttgcgtacac ttaccggaaa agaagggttg
    19501 cgcttattcg gctatccgga cgattattcg tttgatattc ccaaaaaaga cagatgcgat
    19561 ttattgggta ataccgttgc cgtccctgtg attaaggcgg tatctgaaag acttctgcat
    19621 actttataaa ccgacaggga taaaatgata aaacttactg cacaacaaat atttgataag
    19681 ttgctggatg aagaaaaaat cttatcggcc aatggtcaaa ttagattttt cttaggcgat
    19741 gtggatatta tcgtcaaaca aaaagatgtt gtcggcaaca tcattcagga atggctcggc
    19801 ggatgattga gaaaaaggga aattgaattt gatgtttcaa ccaataccca aatgcctccc
    19861 gatttctttt taaataaaaa agaccgcagc agagaattgt tggaagtaaa ggcgttcaac
    19921 cgaaatgccg gcccgggttt tgatattgca gattttaaaa tgtattctga taaaatcatt
    19981 cataagccct atatgctgga tgtagactat ttaatattcg gttacgatat ggacgacaac
    20041 ggcaatgtaa ccatcaagga tttatggctt aaaaaagtat ggcaaattac gagaagcatg
    20101 gatgggtggg caattaatct tcaagtcaaa aaaggcgtgg tgcataaaat ccgcccgggt
    20161 gtttggtaca gcataaataa aaagaatatg cctatgtttg aatgcttgga agattttgtt
    20221 tccgcaattg aagaaaccgt ttatcaaaac ccggcaacac ggcataacgc atccttatgg
    20281 aaaaagaaat ttgaggaagc ctataaaaaa cactacaacc gatcgatttc catacctcgt
    20341 tggcacgaaa ttgcacacaa atacaaaaag aaataaccct tcaaccatca acccataagg
    20401 aaacttgaaa tgaccaaaca tatcgccatc ctccgaggtg acggcatcgg tcccgaaatc
    20461 gtcgccgaaa ccgtccgcgt actcgacaaa cttatcgaac aaggtttgga tgtcagctac
    20521 gaatacgcac ctttgggcgg cgaagcctat gacgaatacg gccatcctta tccagaattc
    20581 acgcaaaacc tttgccgtaa agccgatgcg gtcctgcttg gtgcagtcgg ttctcctcaa
    20641 tacgacaatc tcaaccgtcc gctgcgccct gaacgcggct tattggccat ccgtaaagac
    20701 ttgaatcttt ttgccaattt gcgccctgcc attttgtata aagagctggc caatgcttct
    20761 actttgaaac cagaaatcgt agccggcctc gacatcctta tcgtacgcga actgaccggc
    20821 gatatttact ttggcgaacc gcgcggcatc cgtgttttgg aaaacggcga acgcgaaggc
    20881 tacaacacca tgaaatacag tgaaagcgaa atccgccgta ttgcccacgt tgccttccaa
    20941 tccgcccaaa aacgcagcaa aaaagtctgc tccgtaggca aagccaacgt tttggaaacc
    21001 accgaacttt ggcgcgaaat ctttgaagaa atcggcaaac aataccctga tgtcgagctt
    21061 tcccatatgt acgttgataa cgcagccatg cagctcgtgc gcgcgcccaa acagtttgac
    21121 gtgattgcca ccggcaacat cttcggcgac atcctctccg acgaagcctc catgctgacc
    21181 ggctccatcg gtatgctgcc ttccgcttct ttggacgaaa acggcaaagg cctgtacgaa
    21241 ccgtctcacg gctccgctcc cgacatcgcc ggagaaaaca aagccaaccc gctggccacc
    21301 atcctctcgc ttgccatgct gctgcgttac agcctgaacg acgaagcgcg cgcgcaacaa
    21361 gttgaaaacg ccgtacaaaa agtgctgcaa caaggcttgc gtaccaacga catttacgaa
    21421 gaaggcacaa aactcgtttc ctgctccgaa atgggcgatg cggtactcgc cgccttgtaa
    21481 aaaaacaaag cgcggatcac accgcgcttt ttcatccgcc cttttccgcc cgccccgttc
    21541 gtcaaccgcc tgcaaaccca ccgtttaccg gcaggaaatg ccataaatcc ctgcctatgt
    21601 taggataaac gcctttaccg gcccacacaa aggattatca tcatgaaaaa aatcatcttc
    21661 gccgcactcg cagccgccgc catcagtact gcctccgccg ccacctacaa agtggacgaa
    21721 tatcacgcca acgcccgttt cgccatcgac catttcaaca ccagcaccaa cgtcggcggt
    21781 ttttacggtc tgaccggttc cgtcgagttc gaccaagcaa aacgcgacgg taaaatcgac
    21841 atcaccatcc ccattgccaa cctgcaaagc ggttcgcaac actttaccga ccacctgaaa
    21901 tcagccgaca tcttcgatgc cgcccaatat ccggacatcc gctttgtttc caccaaattc
    21961 aacttcaacg gcaaaaaact ggtttccgtt gacggcaacc tgaccatgca cggcaaaacc
    22021 gcccccgtca aactcaaagc cgaaaaattc aactgctacc aaagcccgat ggagaaaacc
    22081 gaagtttgtg gcggcgactt cagcaccacc atcgaccgca ccaaatgggg catggactac
    22141 ctcgttaacg ttggtatgac caaaagcgtc cgcatcgaca tccaaatcga ggcagccaaa
    22201 caataaatct tgatgcgaat cagcacaaat gccgtctgaa cgtccttcaa acggcatttg
    22261 tgccgtcttc atcaaaccgc cccgccaacg gtaagccgat atggcggagc acaatccgcc
    22321 gaaagatatt tcagcggcag gcagggcttg ccggaaagtc cggaaagtat agtgga
//
LOCUS       DAXIEB010000020        22174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000020 DAXIEB010000000
VERSION     DAXIEB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.020"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(149..967)
                     /gene="cysE"
                     /locus_tag="ACFOA3_000671"
     CDS             complement(149..967)
                     /gene="cysE"
                     /locus_tag="ACFOA3_000671"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6897715.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKECGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1151..1729
                     /gene="grpE"
                     /locus_tag="ACFOA3_000672"
     CDS             1151..1729
                     /gene="grpE"
                     /locus_tag="ACFOA3_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6897716.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPTYED
                     LQARVADLEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQLTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(1853..2068)
                     /gene="nqrM"
                     /locus_tag="ACFOA3_000673"
     CDS             complement(1853..2068)
                     /gene="nqrM"
                     /locus_tag="ACFOA3_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6897717.1"
                     /translation="MKTLLLTFSIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(2094..3149)
                     /locus_tag="ACFOA3_000674"
     CDS             complement(2094..3149)
                     /locus_tag="ACFOA3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6897718.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTTMSSEFEKLLR"
     gene            complement(3301..4518)
                     /gene="nqrF"
                     /locus_tag="ACFOA3_000675"
     CDS             complement(3301..4518)
                     /gene="nqrF"
                     /locus_tag="ACFOA3_000675"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6897719.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(4532..5125)
                     /gene="nqrE"
                     /locus_tag="ACFOA3_000676"
     CDS             complement(4532..5125)
                     /gene="nqrE"
                     /locus_tag="ACFOA3_000676"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6897720.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(5129..5755)
                     /locus_tag="ACFOA3_000677"
     CDS             complement(5129..5755)
                     /locus_tag="ACFOA3_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6897721.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(5755..6531)
                     /locus_tag="ACFOA3_000678"
     CDS             complement(5755..6531)
                     /locus_tag="ACFOA3_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6897722.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(6524..7756)
                     /locus_tag="ACFOA3_000679"
     CDS             complement(6524..7756)
                     /locus_tag="ACFOA3_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6897723.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(7759..9102)
                     /locus_tag="ACFOA3_000680"
     CDS             complement(7759..9102)
                     /locus_tag="ACFOA3_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6897724.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(9362..9424)
                     /locus_tag="ACFOA3_000681"
                     /pseudo
     CDS             complement(9362..9424)
                     /locus_tag="ACFOA3_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9502..10929)
                     /locus_tag="ACFOA3_000682"
     CDS             complement(9502..10929)
                     /locus_tag="ACFOA3_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6897725.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPG
                     FEILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFIT
                     IFPVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNM
                     AGGDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGY
                     MMWSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(11287..11646)
                     /locus_tag="ACFOA3_000683"
     CDS             complement(11287..11646)
                     /locus_tag="ACFOA3_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6897726.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SIFCSLVAIWMWRRPES"
     gene            complement(11643..12248)
                     /locus_tag="ACFOA3_000684"
     CDS             complement(11643..12248)
                     /locus_tag="ACFOA3_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6897727.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFGVFNDMLDIAD
                     TAIIENGLEGIIQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(12295..12777)
                     /locus_tag="ACFOA3_000685"
     CDS             complement(12295..12777)
                     /locus_tag="ACFOA3_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6897728.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      12981..13078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            13289..14389
                     /gene="gcvT"
                     /locus_tag="ACFOA3_000686"
     CDS             13289..14389
                     /gene="gcvT"
                     /locus_tag="ACFOA3_000686"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6897729.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALVA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            14551..14937
                     /gene="gcvH"
                     /locus_tag="ACFOA3_000687"
     CDS             14551..14937
                     /gene="gcvH"
                     /locus_tag="ACFOA3_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6897730.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            15089..16336
                     /gene="hemA"
                     /locus_tag="ACFOA3_000688"
     CDS             15089..16336
                     /gene="hemA"
                     /locus_tag="ACFOA3_000688"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6897731.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            16663..17184
                     /locus_tag="ACFOA3_000689"
     CDS             16663..17184
                     /locus_tag="ACFOA3_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6897732.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHEPIMLIGIPHLPAPGRAIRLNWLPAYIKPNFISTNRLRLKGLPT
                     VLQPSKGTGAGVP"
     gene            17073..18272
                     /locus_tag="ACFOA3_000690"
     CDS             17073..18272
                     /locus_tag="ACFOA3_000690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6897733.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSAEAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEVSGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQVIWRAPVARLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEAETRQSEWGR
                     AGNGSLN"
     gene            18330..18944
                     /locus_tag="ACFOA3_000691"
                     /pseudo
     CDS             18330..18944
                     /locus_tag="ACFOA3_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            18961..19092
                     /locus_tag="ACFOA3_000692"
                     /pseudo
     CDS             18961..19092
                     /locus_tag="ACFOA3_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19089..19583
                     /locus_tag="ACFOA3_000693"
     CDS             19089..19583
                     /locus_tag="ACFOA3_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6897734.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(19671..21332)
                     /locus_tag="ACFOA3_000694"
     CDS             complement(19671..21332)
                     /locus_tag="ACFOA3_000694"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6897735.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(21462..22011)
                     /locus_tag="ACFOA3_000695"
                     /pseudo
     CDS             complement(21462..22011)
                     /locus_tag="ACFOA3_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(21956..22129)
                     /locus_tag="ACFOA3_000696"
                     /pseudo
     CDS             complement(21956..22129)
                     /locus_tag="ACFOA3_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         5338 a         5581 c         6044 g         5211 t
ORIGIN      
        1 cggttgcgtg ggaatcgggt gtatcgaata aaaggcattt tgtccgactg gcaagtgccg
       61 gcatcggcgg catatcaagg cgcaggcttg aagcgggcaa tgccgtctga agcacgtttg
      121 gcgtttcaga cggcattggt gcggatattc aaatcataaa gtcgatttcg gtaaactgga
      181 tattttgatc catatccgcc gacggtgttt tgagcgatcg cgccacgggt ttggcgggta
      241 cgccgacaac cgtgatggac ggcggcacgt ctgaaaccac gacgctgccc gccccgattt
      301 tggcattgct gccgatgcgg atattgccca atatcgaggc gtttgcgccg atcatcacgc
      361 cgtcgccgat tttagggtgg cggtcgccgc attctttgcc cgaaccgccg agcgttacgc
      421 cgtgcaaaat cgaaatattg ttgcccaaca cggcggtttc gccggcaaca aagccggtgg
      481 cgtggtcgag catcagcccg tatccgaaac gggcggcggg atggatgtcc acgccgaata
      541 cttcggacat acggttttgc aggaaatacg ccagcgtttt gcgcccgtcg agatacagcc
      601 gatggttgat gcggtgtgcc tgaatcgcgt ggaagccttt gaaatataaa agcggcagcg
      661 aatattcgtc gcaggcggga tcgcgttcgt agatggcttt taagtctgct tcgacgcatt
      721 tgccgatttg ggtgtcgctg cccaacgcct gctggtagat ttcaaacagc gcgcgcacgt
      781 ccataatcgg gctgccgagt ttgctggaaa ggtggtaggc aaggacggag ccgagggact
      841 cgtggcgcaa cacggtttgg tgcaaaaaac ttgccagcat cggttcggcg gagaccgcgg
      901 ccgcggtttc ttcgcggatg gtgtgccaga ggtcgaaacc ggttgtgttt aaatggtctt
      961 ttttcatgag tgatgacgtt tgaaaatcga tatggtcggc agtatcttac cgtctatatt
     1021 attttttcgg taggggattt gaaaatgaat ttgaaattct ctgcttttgc ttgaagtttc
     1081 ttgaaaatgt ccttatcttg cgcgggtaat aactggattt tgatttccaa tttgttttaa
     1141 gggatacgat atgagcgaac agacacagca gcaaaacagt gaagaagcgg ttgaaaatgt
     1201 ggaggcggtg gaaaccgtcg agacagtagg aaatgcggac ggtgtgcagg agcaggctgc
     1261 cgccgagccg acttatgagg atttgcaggc gcgtgttgcc gatctggaag cgcagttgaa
     1321 agacgagcag ctgcgtgctt tggcaaacga gcagaacctg cgccgccgcc atcagcagga
     1381 aattgccgat acacacaagt tcgccggaca gaagtttgca gtagaaatgt tgccggtcaa
     1441 ggattatctg gaaatggcgc ttttggatca gagcggcaat ttcgatgcgc tgaaaatggg
     1501 cgtgcagctg actttgaacg agttgcagaa agcatttgat gctacgcaaa tcaaggaaat
     1561 caatcctaaa gcgggcgata agctcgatcc gaatatccat caggcgatgc aggcggtggc
     1621 aagcgaacag gaacccaata ccgtggtggg cgtgatgaag aagggttata cgctgtccga
     1681 ccgcgtgttg cgcccggcta tggttacggt ggcgcagaag gaagcctgaa ggcgtctggg
     1741 gaataatctg atttatttcc tgaagcgcgt tttgcgtata aaccgatcga agtaaagcgg
     1801 caatgccgtc tgaacccgcc tgtcgggctt cagacggcat tttgtttcgg cgttaacggt
     1861 caacccgtat gccgcctgcc tgtttttctt catccagttt cttttgcagg gtgtcgcaag
     1921 gtgtgtcgca gtcgcacatt tttttcatac ccaaggcagt aatgccgccg caactgcctt
     1981 tgatgctgcg tttggagaaa atatagccga ccgccatacc gatgatgacg gtcaggaaga
     2041 tgctgaaggt aaggagcagg gttttcatgg tgtttcctaa tcggtttgta tgtttagcgg
     2101 agcagttttt caaattcgga agacatggtg gtgcggtagc cgcctttatc cctgacaatc
     2161 aggaaaacag cgagtttttc gcgctctgcc agctttaagg cttcggtttc tcccaatacg
     2221 aataatcctg tggacaagcc gtccgccgtc atcgcactgt ctgcgaccac gctgatggag
     2281 gcgaggttgt ggctgatggg tcgtttgttg ttcgggttga tgatatggga gaggcgtttg
     2341 ccgtttttat cgacgtggaa aatacggtaa tcgccggaag tggcaagcga acggttgttc
     2401 agcgggacga taatctgcgt attgccgcct tggacgatat tgggctgctc gataccgatg
     2461 cgccacggtt cgccgcgcgc gtttttgcct ttgccgtgca actcgccgcc gatttcgacc
     2521 agataatttt gaatgccgta tttttccagt tcgcccgcaa ctttatcaac gccgaagcct
     2581 ttggcaatcg aagataaatc caaataggcc ttggggtggg ttttgctcaa ggaagcgtaa
     2641 tctttgcctt gtttcaaaat gattttgtct atgcccgtat aagatgccgc ctgtttgatt
     2701 tgttccggcg acggttcacg ggtaacggat ttgtcgggcc cgaatcccca aaggttgacc
     2761 aaagggccga cggttacgtc cagcgcgccg tgtgtcaggc ggttcaggcg gacagcttcg
     2821 gcagtaacgt gtgcgaagtc gcttgaaatg cggaggggtt tgccggccgt ggtttggttg
     2881 aaccggctga tttcggaatt aggctgatag gtggacatct gccggttgac ttctttaagc
     2941 gcgtcatcga tgcgcttttg tatttcggca ggggagggga gtttgtcccg attgtttgaa
     3001 aggtatttga cggtataggt cgtgcccatc gtttcacctt gcagggtaac ggtttgcgcg
     3061 gtttgttccg aacaggcgtt caggaagata aaactcaggg caaatatcaa ggtgcggata
     3121 aagttcggca ggcgtgtttc agacggcata gtgtttgacg gttttggcaa atggtttgaa
     3181 ttatatcgca aaacggccgg tatgtttcta tgccgatgcc gtctgaaagg tgttcggacg
     3241 gtatcggata aaaaaaggaa gaaaccgagg tttcttcctt ttgtgtttga agccgaatat
     3301 ttaaccgccg aaatcgtcca agaggatgtt ttcgtcttcc acgcctaagt ctttaagcat
     3361 tttgatgacg gactggttca tgatcggagg accacacata taaaattcgc agtcttccgg
     3421 cgcttcgtgg tttttcagat ggttttcgta aaccacgttg tgaatgaagc ctgtgtagcc
     3481 gtcccagttg tcttcaggca atgggtcgga cagggcgacg tgccacgtga agttcgggaa
     3541 ctctgccgcg agttggtcaa agtcttcgac atagaacatc tcgcgtttgg aacgtgcgcc
     3601 gtaccagaag gtaatcttac gtttggagtt caaacgtttc aactggtcga aaatgtggga
     3661 acgcatcgga gccatacccg caccgccgcc gataaatacc atttcggcat cggtgtcttt
     3721 ggcgaaaaat tcgccgaacg ggccggaaat cgtaactttg tcgccgggtt tgagcgacca
     3781 gatgtaggac gacatttgtc ccggaggcgc atcaggtacg cgcgggggcg gcgtggcgat
     3841 acgcacgttc agcataatga tgcctttttc ttcaggatac gaagccatag agtaggcacg
     3901 caaaatcggc tcgtccactt tggaaacgta ttgccacaaa ttgtatttgt cccagtcttc
     3961 gtggtattcc tcgggaatgt cgaagtcttt gtaggcaacg gtgtgcggcg gggcttcaat
     4021 ttgaatgtag ccgccggcgc ggaaggggac ttcttcgcct tcgggaatgg caagcttgag
     4081 ttctttaatg aacgtggctt tgttatcgtt ggagatgacg gtgcattccc attttttcac
     4141 gccgaacact tcttcgggga cttcgatgtc catgtcggtt ttgacgttga cttggcacga
     4201 cagacggcag ccttcgcgtg cttcgcgttt gctgatgtgg gacagctcgg tcggcaggat
     4261 gtcgccgccg ccgcttttta cgacgacgcg gcattgtccg cacgaaccgc ccccgccgca
     4321 ggcggagggg ataaagatgc cttcgttggc aagcgcgccc aagagtttgc cgccggcggg
     4381 catcgtcagc tctttttcgc cgttgacttt gatggtgatg tcgccttcgc tgaccagttt
     4441 ggatttggca aacagaatca tcagtgccaa aaccaaaacg atgacggtaa acatcacgat
     4501 acctaaaata atctccatac cgatcccttt cttataactg gatgccagag aacgacataa
     4561 acgccatcgc catcaggccg gcggcgataa aggtaatgcc caagcctttg aggcctttgg
     4621 gagcgtccga atatttcatt ttttcggtaa tgcccgccaa agcgacaatc gccaacatcc
     4681 agcccaagcc cgcgccgaag ccgtatacaa cggactcgcc gaagttgtat tcgcgttgcg
     4741 ccataaacga tacggcgccg aaaatcgcgc agttcacggt aatcagcggc aggtagatgc
     4801 ccaatgcgtt atagagggcg gggacgaatt tatccaagaa catttccaaa atctgcacca
     4861 aagcggcaat cacgccgatg aaggtgatga atttcaaaaa ggtcaaatcc acgccttcga
     4921 caatcgcgcc gtctttgagc agcgagtaaa cgagttggtt gacagggacg gacagcccga
     4981 gtacgaaaat taccgccaca cccaaaccga atgcggtgga tacttttttg gataccgcca
     5041 aaaacgtgca catacccaaa aagaaggata gtgccatatt ttcaatgaag acggatttga
     5101 tgaagaggct caaatagtgt tccatagctt attcctccgc ctgttcgggt ttccaggtac
     5161 gcagtcccca aatcaaaaag ccgatgatga agaacgcgct gggggcgagc aggaacaagc
     5221 cgttggtctg ataccagccg ccgtcctgca cggtttggaa aacggtgtag cccaagagtt
     5281 tgcccgagcc aatcagttcg cggacggtgg cgacgacaag cagcattatc ccgtagcccg
     5341 cgccgttgcc gatgccgtcg atcaggcttt ccagcggcgg ctctttcatc gcaaatgctt
     5401 cggcgcggcc catcacgata cagttggtaa taatcagacc gacgaatacg gaaagctgtt
     5461 tggacaattc gtaggcaaat gcctgcaaga gttggtcaac cagcgtaacc agcgacgcga
     5521 taatcgccat ttgcacgata atacggatgc tgttggggat gtagttgcgt accagcgaaa
     5581 tgaagaagct ggaaaaaccg gttaccaaag ctacggaaat acccatcacg atggccgtct
     5641 gaagtttggt ggtaaccgcc aaagccgaac aaatacccaa aacctgcaag gcaatcgggt
     5701 tgttgtcgat aaagggtgaa aacatcaaat gtttcaagcg tttcatatca gccattattg
     5761 cgctcctgct gatttcaatt tgttcaggta ggggatatag ccgttttcgc cgaaccagta
     5821 ggcgaacgaa ccttgcacgc ctttggatgt cagcgatgcg ccggagaggg catctacgcc
     5881 gtgttctttg tccgaacccg cgcctttgcc gacgtgcagg gcgagtttgc cttgtccgtc
     5941 aaacagtttt ttgccgacga atttttgctg ccacaacgga ttgccgattt cgccgcccaa
     6001 gcccggggtt tcgccttgtt cgtagtaggt aatgccgttg atggtgttgc cgtcgggctg
     6061 gatggcgaca aagccgtaca tgaccgacca caaaccgtta ccgtgcatag gcaggatgat
     6121 ttgcccgatt ttgccgtctt cgccttttac caaataaacc tcggtgtatt tggcacggct
     6181 tttgatgcct gccaaatcgt cttcgggttt gatgcggatg ctttgggcag ggtctttgcc
     6241 tgcgatgcgc gcgctgaagt ctttaggcgc atcggcgacg tattcgccgg tcgccaaatc
     6301 gacaacacgt tgctcgatac gctcggcaaa ggttttaccg atgtcggtgt ccttatccat
     6361 caaaccggct acgctcaaga tatagccttg tttgtcttgg agtttttgtt tctcttggat
     6421 gggtttcaag ccgacgaccg caccggcaac gatgaccgag caaatcaggc tgaccgccaa
     6481 cacgacaatc agcgtgccgc tgaagctgtc tttatcgaat ttcttagcca ttgctgcgcg
     6541 cctttctgcg tttgatgttc gcttgtgcga cgaaatagtc gaaaatcggg gcaaacaggt
     6601 tggcaaacag aatcgccaac atcatgcctt cggggtaagc cggattgacc acgcggatta
     6661 atacgcacat cacaccgatc agtgcgccgt accaccattt gccgacattg gtaaaggaag
     6721 cggaaacggg gtcggtcgcc ataaacagca taccgatggc gaagccgccg acgaccaaat
     6781 gccagtacca aggcatagca aacatagcgt tggtgtccga accgatgaag ttgaacagcg
     6841 aagacatggc aatcataccg atcatcacgc cggcaataat gcgccaagaa gcgatgcggg
     6901 caaacacgat aaacgcgccg ccgattaaga gtgccaaagt ggatacttcg ccgatggagc
     6961 cgggcagttt gccgataaac gcatccatcc aagtgatggt ttgaccggtt acggcgtttt
     7021 tcaggccgtc tgcaccgtgt gccgcccatt gcgccagcgc ggttgcgccg gaatagccgt
     7081 caaccgccgt ccaaaccgca tcgccgctca agttggcagg gtaggcgaag aacaggaagg
     7141 cacggcctgc cagcgcaggg ttcatgaagt ttttacctgt accgccgaat acttctttcg
     7201 caaccaccac gccgaaagaa atacccaaag ctgcctgcca cagcggcagc gtgggcggaa
     7261 cgattaaggc aaacagaatc gaggtaacaa agaaaccttc gttgatttca tgtttgcgca
     7321 cggtggcgaa caaaacttcc cagaaaccgc cgacaataaa tacggtcgcg taaatcggca
     7381 ggaagtaaat cgcgccgaac agcattttgc ccaacacgcc cgcttcagac gacatattga
     7441 tgcccaaagc gttggcaagg gcgtaatgcc agtcgttggc gatgctttgt tgcagcaaat
     7501 cgggcgttaa cgcaccgaat gcctgtgcgc cgacgttgta cataccgtag aacatggcag
     7561 ggaacaaagc cagccacacc aaaatcatca tgcgtttgga gtcgagcgca tcgcggacgt
     7621 gtgccgcttt gcgcgttacc gcaccggatg tatagaaaat cgtcgccgca gcttcgtaga
     7681 gggcatacca tttttcatgt ttgccgcccg gcaggaagtg cggttcgatt ttttccagaa
     7741 aatgtttcaa gcccataatc agccttcctt ctcaatggtt tccagcacct tacgcaacag
     7801 cgggccgtat tcgtatttgc ccgggcagac gaagctgcac aaagcgaggt cttcttcgtc
     7861 caattccaag caacccaatg cctgcgcgct gtcggtatcg ccgacgatta aatcgcgcaa
     7921 aagcagggta ggcaggatgt ctagcggcat tacgcgctcg taagtaccaa tcggcaccat
     7981 ggcgcggtcg ccaccgttga cggctgtcgt gaacttgaag agtttgtttt tcaggaaatg
     8041 gccgagggtc gtacgcgtga tggagtattt gtccggctgc ggcgcaaccc agccgaacag
     8101 ctctttgctg cggccttctt cgataacgga aatctgattg tggtagcgtc ccaaataatc
     8161 gtgcgcgcct tgtgtaatcg cgccgttcaa taccgaaccg gaaatcacgc ggttgtctgc
     8221 gtcaaccaat tcgcccgcag taatttgcga tactttcgca cccaaaacgg tacgcaagag
     8281 gcgtggtttg ttgacttgag aaccacccaa agcaatcacg cgctcggtgt tcagacggcc
     8341 tgttgcaaac aaacgtccga tggcaattac atcttgataa ttgatggtcc aaacggtttt
     8401 gtttgcaccg accggctcaa tgaaatgaat gtgcgtgcca ctcaaaccgg ccggatgcgg
     8461 gccgccgaat tcatgtgttt cgatgttggc agcattttca gacggcacgt ctgcgccagc
     8521 tgccttacac acatggattt tacgctcggt caaacggctc aataccagca aacctcgtct
     8581 gaaatcctcg gcggcttctt tgatcacaac cacagggtct gccgccagcg gattggtgtc
     8641 catcgcattg acgaagatgg cgaacggctc ggcatcgacg gcagggattt tgctgaacgg
     8701 acgggtacgc agcgcagtcc acaaaccgga ttgaatcaga ttgcggcgca cttcttcgcc
     8761 gcttaagttt gccaacgctt cgggcgcgta gcgttcgaac tcgatttcgt cgttgccttc
     8821 aacggcaatc acaaccgact gaagcacgcg cttttcgccg cgatggatgg cggcgatttt
     8881 gcctgaaacc ggcgcggtaa acaccacgcc cggattcttt ttgtcttcaa acagcacttg
     8941 gccttttttg acggcatcgc cttccttgac tttcatcgag gggcgcatac cggcatattc
     9001 ttcgccaagc aacgcgactt cggtaatgac gggcccgtca taaatgactt gctccggtct
     9061 gcccgcgatg ggcaggttta gacctttttt gattttaatc atatatttgc attacttgtg
     9121 atggttaagg taaaaacggc gtgttttgat accgtgtcgc gtggcatcaa aagcattgaa
     9181 taaattaatg tagcaaagtg ttagattcta tcaggaattg tacctgtttg tcagatttgc
     9241 tgcttttttc cttgcggaag ctgtttttat agtggattaa atttaaacca gtacggcgtt
     9301 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
     9361 tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac
     9421 tataattgtc ggaagggaaa aatattgatt tgattatgcc ggaatttaaa atgccgtctg
     9481 aatgttcaga cggcatagcg tttacagcag tttgaaaacg aaaaagataa gggtatgtac
     9541 gatgaagacg ggtgtcagga aggcgaccga ccacatcata tagccgaaga aagtcggcat
     9601 cggtacgccg cgctgttcgg caatggcctt gaccatgaag ttcggtgcgt tgccgatgta
     9661 ggtcagtgcg cccatgaata ccgaacccat agaaaccgcc agcagcgaat gaaacagggt
     9721 acccgtcatc aaggcttggg catcgccgcc cgccatattg aaaaaaacga gataagtggg
     9781 cgcgttatcc aagaatgccg acaatatgcc gctcatccaa aaatacatca cattaatcgg
     9841 atgacctgcc gtatcgtgaa ccagcgatac caccccgccc agcgcgcctg cctcgcctgc
     9901 tttcagaatg ctcaggacgg gaaagatggt gatgaagatg ccgaggaaga gtttgcccac
     9961 ttcggcgatg ggttcaaagt tgaattcgtt gcctgcgcgg acttgtttgg gcgtgattgc
    10021 catagatacg gcggtcaatg caatcaggat gacatcgcgg acgaggtttt gcagggcgta
    10081 acggctgccg aggatttcaa atcccgggtg ttcgggtttc caaaggccgg acattagaac
    10141 cgcgccgacc acgcccgaaa gcaggaggaa gttccatttg ccgaagatgg cgattttttc
    10201 gggtttttcc tgttgttgtt gtgccggcgt atcttgtgca atgctttcct gtttgaagaa
    10261 acggttgtcg atgaaataga aggcggtcaa caggacagcg gtgctgatca ggacgggggc
    10321 gaacatatgt ttgaccgtcc acatgaaatc tacgcctttg aggaagccga ggaagagtgg
    10381 ggggtcgccc aaaggggtca gaccgccgcc gatgtttgca accaggaaaa tgaagaagat
    10441 gacgatgtgc acgcggcggg tacggttttg gttggctttc agcagcggac gaatcatcag
    10501 cattgctgcg ccggtcgttc ccatgataga ggcaagtgcc gtaccgacgg caagcagggc
    10561 ggtgttgagc ttgggtgtgc cgttcaagtc gccccaaacc aaaatgccgc ctgaaatggt
    10621 gtacagggca agcagcagca ggatgaaggg gatgtattct tcaacgagtg cgtgtgcgac
    10681 ggtatggata ccggcggacg cgccaaaaac caaactgaac gggatgagga agagcaatgt
    10741 ccaaaaggcg gtgattttgc cgtaatggtg atgccaggta tgcgaaaaaa acaagggacc
    10801 caatgcgata gacagcaaaa tcagggcaaa gggcaggctc cacagcaggt ttaggtttgc
    10861 gccgtccaaa tctgcggcgt aaaccgatgc ggggaaaagc attagtgaaa acaggggtag
    10921 gtggcgcatc gtgtttcctc gattcaagca ctgccttgcg cggcgcgtgg gagtgataca
    10981 ggcaccgtgc cgcccggaca taggcggact gtgctgcctg tttgtttttg aaaaaagttt
    11041 tccgattgat tgtaaagtaa ataatcagga taaaccagtt tcccaaaccg gaaggcggcg
    11101 ggaaggcgga ttgctgtgct tgggaatata tgttcttttt gataaataat tttatttaaa
    11161 acaaataaat atatgtcttt aataaataat ctatcgaaaa cgaaaaatga atttatttta
    11221 acatatattt gcaatgaaac aggtttgccc cccgtttgtt tgcccttatc cctttcagta
    11281 cggcattcaa gattcgggcc tgcgccacat ccatatggcg acaagggaac aaaaaatcga
    11341 tgaaaccgcc ccgacccacc agcgttgggg aaactgccaa aacatcatca ggcaggatgc
    11401 ggtcatcata ctgatggcga aaatcttggc tttgcgcggc actgcgccgt tttgttccca
    11461 gttatgaacc atcgggccga aatagcggtg ccggtgcagc cagcggtgaa agcgcgggga
    11521 tgcctttgcc cagcaggcgg cggagagcag tacgaacggc gtggtcggca acagcggcag
    11581 aaaaatgccg atgattccca acagcaggga gatgccgccg caggcaatta aaagataacg
    11641 tatcattttg aaatattttt cttattgtgc ggataagggc aggatgtgat accgagtttt
    11701 gcccagcctt catgtcccat tttttccagc agggcgatat tgcgttcgaa tattgccgaa
    11761 gcgtcgggga aggcttgtgc ggctttggca atgctgtctt cgcgtatcag atgcagtgtc
    11821 ggatagggag aacggttggt gtagttgccg atgtcgtccg aatccgtgcc ttcaaattgg
    11881 aaatcgggat gaaacggggc gatttggatg atgccttcca agccgttttc aataatggcg
    11941 gtatcggcaa tatcgagcat atcgttgaat acgccgaaat cggggaatag ggtcgggtga
    12001 accagcaggg tggtttccag ttcggtggcg ggtgtattgc ccagtcgctg cagttcttcg
    12061 tccaagtctt ccaaaaaacc gtcaaggtgt ttggcttcgc tgatcgcgat gcggacaagg
    12121 tttttaacgt ggggggcttt ggcaaaggga cacaggttca gaccgatgac ggctttttcc
    12181 aaccattgtc cggtgtgttc ggcaacagca tctttatttt cggaagtatt gatattcatt
    12241 attgtcatgt aaatgtgttt gcagattgca cgtgcgggaa aatcgggaag ggcactattc
    12301 cttcagcagg tggttgagcg gcagggatgt ggtgtgtttg atttctttta aaacaaagct
    12361 cgattgggca tcctgtacgc cgtggtggga caggagcgta tccaaaacaa aatgggaaaa
    12421 cgcgttcata tcggtaaaaa acgcctgaag caggtagtcg gtttcccctg tcagggcgaa
    12481 gcagctcaag acttcaggcc attttcgaac cgatgcggca aagtcttccc gcgcgtcttt
    12541 tgctttgcgg atggaaacgc ggataaatgc ctgaagcccc aggttgacgg attccggaga
    12601 cagcagcgcg gcatattggc ggacgatgcc ggcatcttcc aactgcttca gacggcgcag
    12661 gcacggagaa ggcgaaagtg cgacacgttc ggacagttcg acattggtca gcctgccgtt
    12721 ttcctggaga acctgtaaga ttttaatatc ggttttgtct aaagtgagtt ggggcatatt
    12781 tgcgttccgt tttaaggaat tcggattgtc tgtccgtatg tttgcggcaa tccgcacaga
    12841 tggagaccat attaacatat aaaaagttat accgtcatcc gggacaaatt ttgttttcgg
    12901 aaaatcatgt gaaaacagag gcggtcggtt tgcatctctt ttaagacggc ttgcccaaac
    12961 cgccgattca agacataatc gggaaatgtg caggagagtg ttacacccaa ctacaatgta
    13021 accaccgaag gcgcagacac ccttaaatcg ctcaggtatc agggactgca cattgaaaca
    13081 aacaatctgg agagcggcgt tggaataacg tccaccgaag gggagaaggc cgtctgaacc
    13141 accattcaga caaccgcgca aagcagtgag cagactggtt tgccatcatg cggatacggc
    13201 cgaaaatctc aggttcaagg acagataggg tcatccgcgc acaggtgcgc gggtggcatc
    13261 tgaacaaaaa atccggagaa acttgagaat gattactctg aaaaccaccc catttcatca
    13321 agcccatcaa gatgcaggcg cgaagctggt cgattttgcc ggctgggagc tgcccatcca
    13381 ttatggttcg caaatcgccg aacacgaagc cgtgcgcacc gacgccggta tgtttgacgt
    13441 atcccatatg ctcgttaccg acgtagcagg cgcaaatgcc aaagcctttt tccgcaaact
    13501 gattgccaac gacgttgcca aactcgcttt tgtcggcaaa gccctttatt ccgctttgct
    13561 caacgacaac ggcggcgtga ttgacgactt aatcgtttac cgcaccaacg aagccgaaac
    13621 ccaataccgc atcgtgtcca acggcgcgac tcgcgaaaaa gatacggcac aattccacaa
    13681 agtcggacaa gagttcggcg tttctttcaa tccgcgctac gacctcggca tgctcgccgt
    13741 acaaggcccg aaagccatcg agaaactcct gaccgtcaaa cccgaatggg cagatgtcgt
    13801 caacaacctc aaaccgttcc aaggcgcgga tttgggcaac gactggtttg tcgcccgcac
    13861 cggctatacc ggcgaagacg gcgtggaagt catcctgccc ggcactgaag ccgtcgcatt
    13921 cttcaaagcc ctgcaacaag ccggcgtaca gccctgcggc ctcggcgcgc gcgataccct
    13981 gcgcatggaa gccggtatga acctctacgg caacgatatg gacgacgata ccagcccgct
    14041 cgaagcaggc atgggctgga cggttgactt gaaagacgaa agccgcgact tcgtcggtaa
    14101 agccgcattg gtggcattga aagaaaaagg cgttgccgtc aaacaagtcg gtttgctgct
    14161 cgacaaaggc ggcatcctgc gcgcgcatat ggaagtgttg accgacaaag gccaaggcga
    14221 aaccaccagc ggcgtattct cgcccagcct gaagcaatcc atcgccatcg cccgcgtgcc
    14281 gaaagacttt gacggcgata ccgccaaagt tctgattcgc ggcaaagaag tggacgtacg
    14341 cgtactgaag ctgccgtttg tgcgcaacgg acagaaacag tttgattgat gcggtttcag
    14401 acggcatttt catttcatat gccgtctgaa agcaggtttt aattgttgtc cgatacggac
    14461 gtttgtagaa agcattgaac aaggcatctg tggatattga ttcatgcaga tgccgtctga
    14521 aaataacccc tatcaatgga gtatcaaacc atgagcaaca acatcccggc cgaactgaaa
    14581 tacgttgcca gccatgaatg gctgcgcctt gaagaagacg gtacgattac cgtcggcatt
    14641 acccaccacg cgcaagagct gttgggcgac atcgtgttcg tcgagctgcc cgaagtcggc
    14701 gcgaaccttg ccgctgaaga gcaagccggt gtggttgagt ctgtaaaagc cgcgtccgac
    14761 gtgtacgcac cgattgcagg cgaagtcgtt gccgtcaacg aagatttgcc aagtgctccg
    14821 gaaactgcca acagcgattc ttacggcgca ggctggttct tcaaaatcaa acctgtcaat
    14881 cctgccgatt acgacggtct gctgactgcc gaacaatacg cgggcgaagt agattaaacc
    14941 gcccggctgc ccgacggcaa ccgccggaca aacggaaact gcaccttcag acggcatttt
    15001 tgcggtcgga ggtgcagttt tttgtccgtg ttttaaggaa gcagttaggc tataataacg
    15061 gtctatattc atctttaccg attttttcat gcaacttacc gctgtcggac tcaatcatca
    15121 aaccgcacct ttaagcatac gggaaaagct ggcgtttgcc gccgccgccc tgcctaaagc
    15181 cgtccgcaat cttgcccgaa gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa
    15241 ccgcaccgag ctttactgcg tcggtgattc ggaagaaatc atccgatggc ttgccgatta
    15301 ccacagtttg ccgattgaag aaatccgtcc gtatctgtac gcgctggata tgcaggagac
    15361 tgtgcgccat gctttccgcg tcgcctgcgg cttggattcg atggtgttgg gcgagccgca
    15421 gattttagga cagattaagg atgcggtcag ggttgctcaa gagcaggaaa gtatgggtaa
    15481 gaaactcaat gccctgttcc aaaaaacctt ttccgttgct aaagagatcc gtaccgatac
    15541 cgctgtcggc gagaactcgg tttccatggc ttccgcttcc gtcaagttgg cagagcagat
    15601 tttccccgac atcggcgatt tgaatgtctt gtttatcggt gcgggtgaga tgattgagtt
    15661 ggttgctact tattttgccg ccaaaagtcc ccggctgatg acggttgcca accggacgct
    15721 ggcgcgtgca caggagttgt gcgacaagct cggtgtcaac gccgaaccgt gcctgctgtc
    15781 cgatctgcct gccattctgc acgattacga cgtagtggtt tcttcaacgg caagccagct
    15841 tccgatagtc ggcaaaggca tggtggagcg tgcattgaaa caaaggcaga gtatgccgtt
    15901 gttcatgctt gatttggcag tgccgcgtga cattgaagcg gaagtcggcg atttgaatga
    15961 tgcctatctt tatactgtgg acgatatggt caatatcgtc caaagcggca aggaggcaag
    16021 gcagaaggcc gccgccgccg ccgaaacgct ggtgtccgag aaggttgccg aatttgtcag
    16081 gcagcagcag ggcaggcaga gcgtcccctt gattaaagcc ttgcgggacg agggcgagaa
    16141 agcgcgcaag caggtgttgg aaaatgcgat gaaacagctt gccaaaggcg caacggcaga
    16201 agaggttttg gaacggctgt ccgtccaact gaccaacaag ctgctgcatt cgccgaccca
    16261 aactttgaat aaggcggggg aagaagataa ggatttggtt cacgccgtcg cgcagattta
    16321 tcatttggac aaataacggt gcgccggaaa aggatgcgtc tgaaggggtt tcagacggca
    16381 tatttgtgtg ccgcctgaca acatcgtgaa atcccacatt atatcgatgt aatcacaaag
    16441 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac
    16501 ggcaaggcga ggcaacgctg tactggttta aatttaatcc actatattat cccgtatgcg
    16561 gattggtttt aagatttgta aatttgattt gcatcaaaaa atcgccgata gatgattcat
    16621 ataatatcaa tattaaagag tatcggtata tcggggatag tcatgtcctg tttttcaatc
    16681 aaacgtatgt ccgcgtttcg ggcgcggata acagcgtttt ttgccgcctt tgtctttttg
    16741 acggcggcac tgcccgctta tgcggagcgt ctgcctgatt ttctggcgaa aatacagcct
    16801 tcggaaattt ttccgggtgc ggaccgttac ggcaagccgg aaggtaagcc tatggttgcc
    16861 cgcgtttaca aaggcgatga gcagttgggc ttggtctata tcacgaccga tgcggtcaat
    16921 acgcgcggtt attcgagcaa accgatcgat acgctgatgg ctttggcaaa tgacggcacg
    16981 atagccggag cgaaactggt cgatcatcac gaaccgatta tgctgatcgg tatcccgcat
    17041 ttgcccgcgc ccgggcgggc gatacgctta aactggcttc cggcgtatat caaaccaaac
    17101 tttatatcga caaaccgatt acgattgaag ggcctgccga ccgttctgca accatcgaag
    17161 gggacaggag cgggcgtacc atagccgtac acgcgccgga cgtaacgctc cgcaacctga
    17221 ccgttacccg ttccggtatg agcctgccag caatggatgc tggtatttat ctcgaagaaa
    17281 ccgccccgcg cgccctgatt gaacacaaca atattttgga taattcggtc ggcgtctatc
    17341 tgcatggttc tgccgaagcg atggtacgcg agaataaaat cgtcggcgat gcgactttgc
    17401 gcgtgaacga gcgcggcaac ggcgttaccg tttggaacgc gcccggcgcg caggtcgtcg
    17461 gcaacgacat ttccaaaggg cgggacggca ttttttccaa caccagcacg cacaacacct
    17521 ataaaaacaa ccgcttcagc gatttgcgtt tcgccgtcca ctatatgtac accaacgaca
    17581 gcgaagtcag cggcaatatt tccgtgggca acaatatggg ctatgtgctg atgttttccg
    17641 agcggctcaa agtgtttgac aatatcgccg tcggcagccg cgatcagggc attatgctca
    17701 actatgtcaa ctattccgat attcacgaca acattatcaa caaggcgggc aagtgcgttt
    17761 ttgcctacaa tgccaactac gataaactgt ccgccaatca ttttgaaaac tgccaaatcg
    17821 gcatacactt taccgccgcc atcgaaggca cgtccctgca tgacaattcc tttatcaaca
    17881 acgaaagcca ggtcaaatac gtcagcacgc gctttctcga ctggagcgag ggcggacacg
    17941 gcaactattg gagcgacaac agcgcgttcg atttgaacgg cgacggcttc ggagacagcg
    18001 cgtaccgtcc caacggcatt atcgaccaag tcatctggcg cgcaccggtt gcacgccttc
    18061 tgatgaacag tcccgcaatc agcatcgtca aatgggcgca ggcgcagttt cccgccgttt
    18121 tgcccggagg cgtggtggac agcaaaccgc tgatgaagcc ttatgccccc aaaattcaaa
    18181 cccgttatca ggcgatgaag gacgagctgc tcaaagaagc cgaaacgcgg cagtcggaat
    18241 ggggcagggc gggaaacggt tctttgaact agtctgcttc agacggcatc cggattcaaa
    18301 tgccgtctga aaacacaaaa ggaacaacca tgaccacaca tcatgtcgaa ttgaggaagg
    18361 taaccaaacg cttcggcgcg caaaaagccg tcaaccaagt cgatttggtt ttgaaggcag
    18421 gagaaagcgt cgggcttgcc ggacacaacg gtgcgggcaa gtccaccatt atgaagctga
    18481 tactcgggct gattaccccg accgaaggcg aagtgatgct tttgggcgaa cgtaccggca
    18541 gcaaagcggg ggcgcggctt cgcagccaaa tcggctacct gcccgaaact gttgcgttgc
    18601 acccttcgct gaccggcatc gaaacgctgg atttttatgc caaacttaaa aaacagccgc
    18661 tcacgcagaa tcgggggctg ctcgagcgcg tcggcatttc acaggcggca caccgccgcg
    18721 tcggcactta ttctaaaggg atgcgccaac gccttgcctt ggcacaagcc ctgctgggcg
    18781 agcccaaagt cctgctgttt gacgaaccga caaccggcct cgaccctgca tcaaggcaaa
    18841 tgttttacga agtcgtgcgc gaactcaacg ggcacggcgc gaccgtattg ctcagtaccc
    18901 acgcccttgc cgagttggac gggcacgccg accgcattat cgtggattaa atttaatcca
    18961 ctatatgcgg gtatggcggg tttgagcgga caaatcagcc tgaccgtccc cgttttgctg
    19021 accgctcagg ttttatgggt tatcattccg cttgttttgg cagccggaat ttttagaaag
    19081 cgacaaatat gaaaaaaacc ctgttggcaa ttgttgccgt ttccgcctta agtgcctgcc
    19141 ggcaggcgga agagggaccg ccgcctttac cccggcagat tagcgaccgt tcggtcggac
    19201 actattgcag tatgaacctg accgaacaca acggccccaa agcccagatt ttcttgaacg
    19261 gcaaacccga tcagcccgtt tggttctcca ccatcaagca gatgttcggc tataccaagc
    19321 tgcccgaaga gcctaaaggc atccgcgtga tttacgttac cgatatgggc aatgttaccg
    19381 attggacgaa tcccaatgcc gacacggagt ggatggatgc gaaaaaagcc ttttacgtca
    19441 tcgacagcgg ctttatcggc ggtatgggtg cggaagacgc gctgccgttc ggcaacaaag
    19501 agcaggctga gaaatttgca aaggataaag gcggtaaggt tgtcggtttc gacgatatgc
    19561 ctgataccta tattttcaaa taatattgaa aaaaccggcc gaaactttaa aaacgagttt
    19621 cggtcggttt ttcttttctt gttcggtata gtgtcggcag gaaagaacct tcacatcccg
    19681 ccgtaattcg gcccgctcgc gccttcgggg caaatccaag tgatgttttg cgtcgggtct
    19741 ttgatatcgc aggttttgca gtgcacgcag tttgccgcgt tgatttgcag gcgcggattg
    19801 ccgttttctt caacaatttc gtacacgccg gccggacaat agcgcgtttc gggcgaggcg
    19861 tattctttgt agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
    19921 tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
    19981 acgccgtcgg gtttcggata atcaatcggc ttgcacgcgg cggctttttt gagctgctcg
    20041 ttgtctttgc cgtgatgttt caaggtccac ggggctttgc ctctgaaaat catctgatcg
    20101 atgccggtgt agattgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
    20161 gcggcgtaaa gctcttgata cagccagctt tgttcaaaac gttgctgata atccgccgcc
    20221 tctttgccgc tgtcgaaacc ctccacttct tcaaggtttt ccaacaaggg gaacacggct
    20281 tcggcggcga gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg cggcatattg
    20341 aggaaacccg ccgcatcgcc gaccaaaacg ccgcctttga acgagagctt cggcaaactt
    20401 tgaaaaccgc cttcaatcag cgaacgcgca ccgtaagcaa tgcggcggcc gccttcaaag
    20461 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
    20521 ggattttgat agtccaaacc gaccacgaag ccgacggcaa ctttgttgtc gtcgaaatgg
    20581 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcatcacc
    20641 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
    20701 gtttgcggct ggctgttttg gtcgagtttg aagcgttcga tgatttgttt ggaaagcgaa
    20761 cctcggcagc cctcggcaaa cagggtttgc cgtgcccaaa gctccatgcc gggctggaat
    20821 gaatcggtcg gctcgccgtt tttgcccacg cccatattgc cggttgcaat gcctttgacc
    20881 gaaccgtctt tgtgatacag cacttcggca gcggcaaagc ccggataaat ttccacgccc
    20941 aaattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
    21001 ccgtgattgt cgaaattggg ggtaatgggc agattgaacg cttttttctc cgtcaggaac
    21061 aacaccttgt cctgcgttac cgtgcgcgtc agcggcgcgc ctttttcttt ccagtcggga
    21121 atcaactcat tcagcgcaat cgggtcaatg accgcgcccg ccagcgaatg cgcgcccgcc
    21181 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgttctgttc ggcaagctgc
    21241 ttgagtttga tggcggcgga caaacccgat gggcctgcgc cgacaatcac gacatcgtat
    21301 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
    21361 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
    21421 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
    21481 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
    21541 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
    21601 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
    21661 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
    21721 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
    21781 agggcatagc ctttcgcctc aaaacccaaa tcaggcatga tgcgccgcat atcctcaaac
    21841 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
    21901 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
    21961 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
    22021 agcaacagga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
    22081 ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt tttgttaatc cactataggt
    22141 ttccgtgcgg acgtgttcag attcccgcct tcgc
//
LOCUS       DAXIEB010000021        21758 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000021 DAXIEB010000000
VERSION     DAXIEB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.021"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            57..1610
                     /locus_tag="ACFOA3_000697"
     CDS             57..1610
                     /locus_tag="ACFOA3_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6897736.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1700..3727)
                     /locus_tag="ACFOA3_000698"
     CDS             complement(1700..3727)
                     /locus_tag="ACFOA3_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6897737.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEIGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQRELSEEYGLSVR
                     VDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4016..6022
                     /locus_tag="ACFOA3_000699"
     CDS             4016..6022
                     /locus_tag="ACFOA3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6897738.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHILHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(6250..7341)
                     /gene="mltB"
                     /locus_tag="ACFOA3_000700"
     CDS             complement(6250..7341)
                     /gene="mltB"
                     /locus_tag="ACFOA3_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6897739.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAVNANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSSSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(7490..8917)
                     /locus_tag="ACFOA3_000701"
     CDS             complement(7490..8917)
                     /locus_tag="ACFOA3_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6897740.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKIIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLSNDIRKDILDCRYCG"
     gene            9102..12800
                     /gene="mfd"
                     /locus_tag="ACFOA3_000702"
     CDS             9102..12800
                     /gene="mfd"
                     /locus_tag="ACFOA3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6897741.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSSDVFAGRLKNYGQVLP
                     DIFGVEHTLPNVAVNRQADEPLQALKDFQTAFKGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAEINIG
                     DPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMVDGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            12949..13332
                     /gene="panD"
                     /locus_tag="ACFOA3_000703"
     CDS             12949..13332
                     /gene="panD"
                     /locus_tag="ACFOA3_000703"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6897742.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            13354..14196
                     /gene="kdsA"
                     /locus_tag="ACFOA3_000704"
     CDS             13354..14196
                     /gene="kdsA"
                     /locus_tag="ACFOA3_000704"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6897743.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYAEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            14211..14651
                     /locus_tag="ACFOA3_000705"
     CDS             14211..14651
                     /locus_tag="ACFOA3_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6897744.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAALVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            14699..15985
                     /gene="eno"
                     /locus_tag="ACFOA3_000706"
     CDS             14699..15985
                     /gene="eno"
                     /locus_tag="ACFOA3_000706"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6897745.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGNKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            16000..16278
                     /gene="ftsB"
                     /locus_tag="ACFOA3_000707"
     CDS             16000..16278
                     /gene="ftsB"
                     /locus_tag="ACFOA3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6897746.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(16319..16609)
                     /gene="yfaE"
                     /locus_tag="ACFOA3_000708"
     CDS             complement(16319..16609)
                     /gene="yfaE"
                     /locus_tag="ACFOA3_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6897747.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(16807..17940)
                     /gene="nrdB"
                     /locus_tag="ACFOA3_000709"
     CDS             complement(16807..17940)
                     /gene="nrdB"
                     /locus_tag="ACFOA3_000709"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6897748.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(18154..19134)
                     /locus_tag="ACFOA3_000710"
     CDS             complement(18154..19134)
                     /locus_tag="ACFOA3_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6897749.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(19100..21379)
                     /gene="nrdA"
                     /locus_tag="ACFOA3_000711"
     CDS             complement(19100..21379)
                     /gene="nrdA"
                     /locus_tag="ACFOA3_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6897750.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQAPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            complement(21536..>21758)
                     /locus_tag="ACFOA3_000712"
     CDS             complement(21536..>21758)
                     /locus_tag="ACFOA3_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897751.1"
                     /translation="NNKFQSVRKWNLSAAARQNHTLQNLTIRRGRKPEAFLFRRKILI
                     SPPCSRSRSKGKKFTRFLSVKNYQIKQIL"
BASE COUNT         5360 a         6141 c         5304 g         4953 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttct ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacgaaatgc tgaccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgacggc aaggaaaaaa ccgcctaccg cgcgctcaag caggaagccg
      181 aagccgtcgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc catctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggccg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc cgtcgaaaaa atcatttgga cggacaaaag ccgtccgacc ggcgaaatgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gccaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgttcgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgtttttcc gttttccaac gttttgaaac aggccctgct caaacgcgcg accgtgtttt
      841 tgggcgtacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg catccgcctg tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc
      961 tcgattttaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctcgcccgt tgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagtc aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgatcgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg ctttatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaacgtcta tccgcgcgaa atcgaagaag aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaatgcg gtgaatctgc ttcaccgcat ttttttggca
     1681 aacgccggag ataaggcact tactctgcca attccacctg ctcgtgcgcc atcagttcct
     1741 gaccgacatc atccaacaac atcaaataac gttcgtagtt tttcaaaatg tcggcaatca
     1801 gctcgtcctt gttcatatac tgcacatcgt acccgacgcg cccgtcgaaa aaataagcgt
     1861 agggtttgta agttgtctgg tgccggatat gcggcagctt gccgtcgtta atcaactggt
     1921 cggatacatc ctgcccgaca gatttaatcc cgtacataaa atcgcgcatc gtctctttcc
     1981 gaatgacgaa ctcgattgcg ggctcgtccc gatgaaacat tttatcgacc cggacgctca
     2041 agccgtattc ttccgaaagc tcccgttgca actcgtgcat agcgggcgat gcagtctgtt
     2101 tgaggaattt taaaatatcc tgctcctgcg tctggctcat tatctgcacc agccgttctt
     2161 tccacttgcc gcccgtccaa aatacactgg tagggttaac ccgggtctca aaatatttct
     2221 tatccgcgct caatcctttc cacaggctga aacacattat cagcatcagc agggcaaacg
     2281 gcagggaaac aatcagggtc atagactgca ggttgccgag tccgcccgag cgcatcagca
     2341 aaacggcaac ggcagacatc agcacgcccc acataaccgc ctgccaccgt ggcgcgctca
     2401 agcctttgtc ccgagaggta atattgttca ggacataaat cccggaatcg gcagaagtta
     2461 caaaaaacag agaaatgacc agcaggctga cgatgctcgt caattcgggc agggggaggt
     2521 aattaaagaa tttaaaaagc agcgtttccg gagaggaggt catcttttcg agcattcccc
     2581 ccgcaacccc gtcattcagc caaatcgccg tattgccgaa gacggtaaac cacaaaacgc
     2641 cgaacaggcc ggggatgagc aaaaccccga agacaaactc gcggatggtg cgcccctttg
     2701 aaatgcgcgc gataaacaaa cccacaaacg gcgcccaaga acaccaccac gcccaataaa
     2761 gcaccgtcca agattcaaac cacggcttgt gttcccgttc gtacgcataa gttttaaaac
     2821 tgaggcgcac cagatttccg aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt
     2881 aaacagtggg acccgccgcc aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca
     2941 actcgctcaa caccttcacg cccttcccca cgccggatat tgccgaaacg acggcgaggg
     3001 acatgacggc ggcgataatc aaaacctgca cgctgaagct gttttcggca atccagccta
     3061 tttcctgcaa tccggcgccc agttgcgaag ccccgaaccc caatgtggtg atgatgccga
     3121 aaaaagtagc aagcaacgcc ataatatcaa tggcatcgcc gaaccttccg gaaatttttt
     3181 ctttcaacag ggggtaaaaa caagaacgca gggcaagcgg cagcttgtag cggaaaccga
     3241 aataagccaa agccaatgca atcgtaccgt acaccgacca agcgtgaacg ccccaatgga
     3301 acaccgtgtg cagcaatgcc tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg
     3361 aaaaataatg catcaacggc tctgccacgc cgaaaaacat cagacccacg cccatcccgg
     3421 ccgcaaacag catcgccagc cacgacagga agccgaattc cggcacatct tcatcccgtc
     3481 cgagcctgat gtttcccaaa ctgctgaccg agagtatcag caggaaaccc agaaaaatgg
     3541 aaaacgttaa aacataaaac cagctgaact cggtaaaaat gacttctttt gcccgatcga
     3601 gccacatctg cacctgatcc ggcacggtta aaaccaatac caccaaaaca cacacaaaaa
     3661 acaaagtcgt caaaataacc atcggattaa atgacgttcg gcgttctata aattcagaca
     3721 gggacaaacc ttctcactcc tttgttaaaa acagacaaac ccagtcatcg ggcaaagcgg
     3781 tcaaaacctg ccggtaaaat accggcttcc ggatgacgaa atgcacaaac cggccgaaat
     3841 tgttataatc gaaaaaatta aaaatcaata cggattattc tatcagattg atttatcgat
     3901 atttattata tcattaatat agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc
     3961 aggcgcgccc tccccgctat cggggcgttt aacgccggat tgccgatgcg gtacaatggc
     4021 tgatatgaag aaaattaccc ctcaaaacct gcgccccctg ctttcggaaa gcttgggaca
     4081 taccgatttt gtcaacgtcc tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa
     4141 atgtgcgggg gaacgtttcg acctgattat cgacacattc aaacaagaca gggaattact
     4201 gtcccgcttc agccggtgtt tttacatctg gctcgcgcaa atacacattt atccggcact
     4261 catcaaactc ggcatcttct cgcgccacag ctttgcccgg gaaatgggca tacgcatcta
     4321 cgaacgcttc agcccgtcat ataaagattt tgccaacttg ggcgaagtct tcctttatct
     4381 tttccattcc gaaaacgacg acaaatggct gcaaacgctc aatatccgcc aatggctggt
     4441 tttatacgaa ctcatccgga gccacgccga gccgtccaaa ttgcagacgg cgggcatccg
     4501 ccttgccgat gcgcgtttgc gcgccatcga aatgctgtct gtctggacgg catccgaagc
     4561 catcgaaccc gacctcatcc gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt
     4621 cgccctccaa cgcgaaaccg ccaaactggt cgaacactac cgcaacggca ccgcgcctta
     4681 cgacaccgcc cacctcgaag tgatgttcga ccaatgtttc agccagattg actatttgcg
     4741 ccgcaaaggg acgggcgccg gctctggttc gtcggtcaaa gtcgcccacc tgctcgaacg
     4801 gctccggcag accgtagacc gtctgaagct gctcaccgac atccaaaccg gtgccggcaa
     4861 cagcaaccgc ctgaccatcg cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata
     4921 cagcacccgc cacctgcgcc gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa
     4981 caaaagccac cacggcgaac actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt
     5041 ctactcggcg gcaggcggcg gcgtcatcat cgccctgatg gcgctgctca aaatccgcat
     5101 cggcacactc ggcctcagcc ccttcctcac ttccttgtcg gcggggttca actacggcat
     5161 cggctttatg atcatccata ttctgcactg caccgtcgcc accaaacagc ccgcgatgac
     5221 tgccgccagc ttcgccgaac aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa
     5281 gctcgccaag ctcctcatcg acgtatgccg ctcccaaagc gtcgccgttt tcggcaacgt
     5341 ttccatcgcc atccttttgg cgtgcgccat atcgttcggc tatgcccatc tgcaccaaca
     5401 gcccatactc gatgcccaca ccgccgccta ccagttcaaa tccatagaca tcatcgctta
     5461 cccgacgctg tggtatgccg ccattgcagg tctgtggctg ttctgctccg gcatcatcgc
     5521 aggttttttc gacaaccgcg ccgactacct caacctgcgc caacgcctgc ccttcaaccc
     5581 cttgctgcgt aaaatcatgc gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa
     5641 acactacggc tcgttggcag gcaacttcat cttcgggatg ctcttgggta tgaccggcta
     5701 tttcggacac ctcctcgggc tgccgctgga cattcgccac gtcgccttct cctccgccaa
     5761 cctcggctat gctgccgtca gcggcaacgt cggtttgggc acgttcgtac tcggcatttt
     5821 cagcgtcctc gccatcggct tggtcaacct ctgcgtcagc ttcagcctcg ccctcttcgt
     5881 cgccctgcgc tcgcgcggca cgaaaatcgg cagtatccgc aatctgatta agagtttttg
     5941 gaatcagatt aaaagcaatc cctgcatact tttcctcccg cccgccaaag aacagggaca
     6001 tcctccttcg gacaagcctt aaccggcaat gccgtctgaa gcgggattcg ccccgaatac
     6061 cgccctgatg cggaaaatcc ccataaaggg atgcaaaaat gccgtccgaa ccgcgtttcg
     6121 gttcagacag cattttatag tggattaaca aaaatcagga caaggcgacg aagccgtaga
     6181 cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gttcaaattt aatccactat
     6241 aaaaaaacat tacaatcccg ggccgccgag cgaattggca atgtccctga ccgccgttac
     6301 atacatccga ctgtggttgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
     6361 ttcaaacacg ccgggtgcgg tttccagttt gaacaaaacc gccttttcat catcggcaag
     6421 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
     6481 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagtat
     6541 tttcccgccc gtgcgccaac cgtgctgctt catataattg gcaaccgatg ccgcgacatc
     6601 gccgatgttg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
     6661 gtagctcgaa ggcataaatt gcggcatccc cattgcgccc gcatagctgc ctttaaaggc
     6721 gaaaacatcg ccgccttctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
     6781 aaacccggcg cggcgggggt aatcaaagcc taaggtcgcc aatgcgtccg ccacacggaa
     6841 actgcccgta tttttgccgt aattcgtttc aatcccgata accgccacga taagttcggc
     6901 aggcacgccg tatttttgcg ccacatcatc gataagtgcg cggttttccg cataaaaccg
     6961 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
     7021 cgatgaggag gggcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
     7081 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
     7141 gcggacattt gcattgacgg caaacccgct gtcggatacc ggtacggctg ccgcgtcaaa
     7201 cgcggggcgg ctttcttttt tcatttcatc cgcgcggggg gcttgggctt catttacccg
     7261 gggcgggcgt gcctccatcg ccgtacaggc agacaaagcc gccaaacaaa ttgccagcgg
     7321 cagtattttt ctctttttca taaatatgtt ccgaacaaat agggtaagtg ggaaagcggc
     7381 gcaaggggcg cggcgcgaaa tgcggcatcc gccgcaatcg gcggctttgt ggaataccgc
     7441 gcgttgcctt ttgcaccgcc tgaaatccgt atgccgccgt atttgcccgc tatccgcaat
     7501 atcggcagtc caatatgtct ttgcggatgt cgtttgacag gaaggctgcg gtgtcttcgt
     7561 gttccgcctg tacgaagaca aagagtcggg cgatgatttt gccgataaag actttttgca
     7621 gggcgcaggc atcggtcagg gtgtgttcct gcaggaagct gcggatgtct tcgggcaagg
     7681 tgtagtcgcg ggcaacggcg gcaaagcggg catcggtttg caggcggtcg agcagggttt
     7741 ggtttttgtc caacttcatg cctgcttctt tttcttcggc ggtggcgcgg acaaactggt
     7801 cgtaaatttc ggcataaagc atatcgttcg ggccttcaaa aatcgtgaag ggacggatgt
     7861 cgatggcgat attgccggcg gtgtgtccgc gttcaaaacc cttcgcgccc aagagttttt
     7921 gcaacatttg cgcggcggca taagtgtatt ccgtggcgag ggttttgacg atgttcgcct
     7981 ccatcagttg atgggcgacg ggtgcaacgg gcgaaacgga atggcagacg tagcggtaaa
     8041 gaatctcgga aacctgatgg cggcgccgga tttcgcggcg ttcgtaatcg acgaatctga
     8101 tgtcgttgcg gacgtatcgt tccaggtttt caaggatgta ttccataatg ccgtgcgtca
     8161 tgccgatcag ttgcaggcgg ctgcggataa agatgttttg gaacgcgcgc aaaccggcag
     8221 cgtcgctctg ggagagtttc atcacggcgg ttgcaggcat ttcggcatcg atgcggttga
     8281 cggcgtaacg aacggcgcgc aagccttcgg atgcgagggt ttcgcagcgg atgtatgttt
     8341 tggggacgag cagcaggtcg atgattttgg cgagtttgcc gtttttgcgc tctttggcgg
     8401 caacgaggag gaagtcgctt tgcgagttac cctgccagta tttcgcggcg ttgacgtaaa
     8461 tggtttgtcc gtcggtatat tcgtagtaag actgcatttc gcgtgcaatc gccgcgccgg
     8521 aggtttcggg ttcggtaacg cccaaaccgc cgccctcgcc tttgaaaacc atgtccaaac
     8581 cctgtgcgat ttgcgcttca tcgccgaact cttgcagtgg ctgcaacacc agcgcgcctt
     8641 cgatgccggt acgcagcgta acgggcacgc cgtaatgccc cgcaatccgc aagacttcct
     8701 gaatttcaaa ctggctgccc ttgcggccgc cgtgtttttt gtcgaggaag ggcaacagca
     8761 aacccgcctg cttcaaggca agccatttgt cttcgggcag gtatcgcatc aggtcgatac
     8821 cgtctgaaaa aatgcggcgg aatgcggatt cgatgtgctt taaaaaagca gccgtgtcca
     8881 tagttgacgg ctgcgcgctc ggttcggtgt gtatcatcgg cttcctctgt cggttcccat
     8941 taatcggcgg ccggtcaaac cgcctgccac agtttagagt tgattttcta aactttacca
     9001 caaagtgcgc cgggcaacaa tccgccgacc tttcagacgg catcgcgccc cctcccgtgc
     9061 taaaatgacc gtttgcatca ctgtccgccg attgccgcac tatgacctac cccatcccca
     9121 aaccccgtga aaaatcccgt tggtccaatc tttcgcaagg ctcgctgccc ttggctttgg
     9181 cgcgttatct gccgcacaag cggctcaagg tcgtgctgac ccaagatgcg gaacaggcgt
     9241 tgcgccttca gacggcatgg cggtttttcc gtccgcacga tacggcggtg ttcctgccgg
     9301 actgggaaac gctaccttac gagcgttttt ctccgcatca ggatttggtg tcggagcggc
     9361 tgtcggcgtt gtggcagatt aagagcggcg cggcggacgt gttgtttgtg ccggttgcca
     9421 cggcgatgca gaagcttccg cccgtgccgt ttttggcggg gcgcacgttt tggctgaaaa
     9481 caggacagac tttggatata ggccgtctga aaaccgattt ggtagatgcg ggctacaacc
     9541 atgtttccca tgttgttgcg gcgggcgagt ttgccgtgcg cggcggcatt gtcgatttgt
     9601 tccccatggg cagcgaaatg ccctaccgca tcgatttgtt cgacgatgaa atcgacagca
     9661 tcaaaacctt tgatactgaa acgcagcgca ccatttctcc tgtgtctgaa atccgcctgc
     9721 tgccggcaca cgagttcccc accgacagcg aagcgcaaaa aatcttccgc agccgcttcc
     9781 gagaagaagt cgacggtaat ccgaacgatg cggctgtgta caaagccgtc agcaacggcc
     9841 acttcggcgc gggcgtggaa tattatctgc cgctgttttt tgaaaatgag ctggaaacgc
     9901 tgtttgacta tatcggcgaa gacgcgctgt ttgtctcttt gggcgatgtt cacgccgagg
     9961 caaaccgctt ttggagcgac gttaaatcac gttacgccat ggcgcagggc gacgaaacct
    10021 atccgccttt gcttccacag catttgtatc tctcctcaga tgtttttgca ggccgtctga
    10081 aaaattatgg tcaggtattg ccggatattt ttggtgttga acatacgttg ccaaacgtgg
    10141 ccgtcaaccg ccaagcggat gaaccgttgc aggcattgaa agattttcag acggccttta
    10201 aaggacggat tttgttgtgc gccgaaagtt tgggacgacg cgaaaccatg ctcggtttct
    10261 tgcagcaaaa cggtttgaaa gccaaacccg tgtccgactg gcagggcttt ttatcggcac
    10321 acgagccgct gatgattaca gtggcgccgt tggcatacgg gttcaaactg ggcggactgc
    10381 aatcgccgaa ccaacagcaa actacttccg cctccgaggg agaaggcgat gcggttaccg
    10441 accaaaccga attttccgca tccgcaacaa accctctccc tagccctctc cctcaggaga
    10501 gggaacaaag tgcagccgtc gtttcagaca gtctgaaagc agccgccgtt tcaaccgaaa
    10561 gcagcctgcc ccccggtaaa agtaatctgc acgggcaaat ccaacagcaa cctgccccct
    10621 cccccgtggg ggagggttgg ggagaaggca aagcagttgc cgctcaaagt gccatcgccg
    10681 tcatcaccga atccgatctc taccaatacg tcgcccgttc gcgcatccac aaccgccgca
    10741 agaaacacgc cgccgtttca gacgacctgt tgcgcgacct tgccgaaatc aacatcggcg
    10801 accccgtcgt gcacgaggaa cacggcatcg gccgatatat gggcttgata acaatgaact
    10861 tgggcggcga aaccaacgaa atgatgttgc tcgaatatgc gggcgaagcg cagctttatg
    10921 tgcctgtttc acaactgcat ttaatcagcc gctactccgg tcaggcgcat gaaaacattg
    10981 ccctgcacaa gctcggcagt ggcgcgtgga acaaggcgaa gcgcaaagcc gccgaaaagg
    11041 cgcgcgatac cgccgccgag ttgcttaacc tttacgcccg acgcgctgcc caatcgggac
    11101 acaagtttga aatcaacgag ttggattatc aggcgtttgc tgacggcttc ggctacgagg
    11161 aaaccgaaga ccaggccgcc gccatcgccg ccgtgattaa agatttgacg caagcgaagc
    11221 cgatggatcg ccttgtgtgc ggcgatgtcg gtttcggcaa aaccgaagtc gccctgcgcg
    11281 ccgcgtttgt ggcggtgatg ggtggcaaac aggtcgccgt actcgcccca accacgcttc
    11341 tggtcgagca gcacgcgcaa aactttgccg accgtttcgc cgatttcccc gtgaaagtcg
    11401 ccagcctttc gcgtttcaac aacagcaaag ccaccaaagc cgcgctcgaa ggcatggtgg
    11461 acggcacggt cgatattgtt atcggcacgc acaaattggt gcaggacgac atcaaattca
    11521 aaaacttagg tttagtgatt atcgatgaag aacaccgctt cggcgtgcgt cagaaagaac
    11581 agctcaaacg cctgcgcgcc aatgtcgaca tccttaccat gaccgccacg ccgattccgc
    11641 gcaccctcag catggcactc gaaggactgc gcgacttttc gctgattacc accgcgccca
    11701 gccgccgcct tgccgtcaaa acctttgtca aaccctttag cgaaggcagt gtgcgcgaag
    11761 cagtattgcg cgaactcaaa cgcggcgggc aagtgttttt cctgcataat gaagtggata
    11821 ccatcgaaaa catgcgcgag cggctggaaa ccctattgcc cgaagcccgc atcggcgtgg
    11881 cgcacggaca actgcgcgag cgcgagctgg agcaagtgat gcgcgacttt ttgcagcaac
    11941 gatttaacgt gttgctctgt tccaccatca tcgaaaccgg catcgacatc cccaacgcca
    12001 ataccatcat catcaaccgc gccgataagt tcgggctggc gcaattgcac cagcttcgcg
    12061 ggcgcgtcgg ccgcagccac caccaagcct acgcctacct gctcacgccc gaatacatca
    12121 ccaaagacgc agaaaaacgt ctcgatgcca tcgcggcggc agacgaactc ggcgcaggtt
    12181 tcaccctcgc catgcaggat ttggaaatcc gcggcgcagg cgaaatcctc ggcgaagggc
    12241 agtccggcga aatgatacag gtcggcttca cgctctacac cgaaatgctc aaacaagccg
    12301 tgcgcgacct caaaaaaggc cgccagcccg acctcgacgc accgctgggc atcaccaccg
    12361 aaatcaaact gcacagcccc gctctgctgc ccgaaagcta ctgccccgac atccacgagc
    12421 gtctcgtcct ctacaaacgc ctcgccgtct gcgaaaccgt gcagcaaatc aacgccatac
    12481 acgaagaact cgtcgaccgc ttcggactgc ccgaacaacc cgtcaaaacc cttatcgaaa
    12541 gccaccacct gcggcttatg gcaaaagaat tgggtatcga tgccattgat gcggccggcg
    12601 aagcggtaac ggtaaccttt ggtaaaaaca ataatgtcga tccaaccgaa atcatcctgc
    12661 tgattcagaa cgacaaaaaa taccgccttg ccggcgccga taagctgcgg tttaccgcag
    12721 agatggaaaa tatcgaggtc agaatcaaca ccgtaaaaaa cgttttaaaa accttgcaaa
    12781 acagatgcct gcccaaataa agccgacacc gcaatgccgt ctgaaacacc gttttccttg
    12841 tccgaaagcc gccattatga atttgaagga aactccacta taatacggca ttcagatttc
    12901 cagacggcat cgcgcccgtc aaaccgcaca caaaccaaaa ggaaatacat gttccgtact
    12961 atgcttggcg gaaaaatcca ccgcgccacc gttaccgaag ccgatttaaa ctatgtcggc
    13021 agcattaccg tcgatcaaga cctgttagac gcggcaggca tctaccccaa cgaaaaagtc
    13081 gccatcgtca acaacaacaa cggcgaacgt tttgaaacct ataccattgc agggaaacgc
    13141 ggcagcggcg tgatttgtct gaacggtgct gcggcaaggc tggtgcagaa aggcgatatc
    13201 gtcatcatca tgtcttacgt ccaactctcc gaacccgaaa tcgccgcgca tgaacccaaa
    13261 gtcgtcttgg tagacggaaa caacaaaatc cgcgacatca tctcctacga gccgccgcac
    13321 accgtgctgt aaccctgaaa acggacattg accatggata ttaaaattaa cggcattacc
    13381 gtcggtaacg acgctccttt tgtactgttc ggcggcatca acgttttaga agatttggat
    13441 tccaccctcc aaacctgcgc acattacgcc gaagttaccc gaaaactcgg tattccctat
    13501 atctttaaag cctctttcga caaggcaaac cgttcctcca tccattctta tcgcggcgta
    13561 ggcttggaag aaggcttaaa gatttttgaa aaagtcaaag cagagttcgg catccccgtc
    13621 attaccgacg tacacgaacc ccatcagtgc caacccgtcg ccgaagtgtg cgatgtcatc
    13681 cagcttcccg cctttcttgc gcggcagacc gatttggtgg ccgcaatggc ggaaactggc
    13741 aatgtcatca acatcaaaaa accccagttc ctcagcccct ctcaaatgaa aaacatcgtg
    13801 gaaaaattcc gcgaagccgg caacgggaag ctgattttat gcgaacgcgg cagcagcttc
    13861 ggctacgaca acctcgttgt cgatatgctc ggtttcggcg tgatgaaaca aacctgcggc
    13921 aacctgccgg ttattttcga cgttacccat tccctgcaaa cccgcgatgc cggttctgcc
    13981 gcatccggcg gtcgtcgcgc acaggctttg gatttggcac ttgcaggcat ggcaacccgc
    14041 cttgccggcc tgttcctcga atcgcacccc gatccgaaac tggcaaaatg cgacggcccc
    14101 agcgcgctgc cgttgcacct tttagaagat tttttaatcc gcatcaaagc attggacgat
    14161 ttaatcaaat cacaaccgat tttaacaatc gagtaacacg gtttcgcctt atgatgcaga
    14221 ctttccgaaa aatcagcctg tatgccgcaa ccttgtggct cggtatgcag attatggcgg
    14281 gttatatcgc cgcactggtg ctgttcaaaa tgctgcccaa aatgcaggcg ggcgaaattg
    14341 ccggcgtatt gttcgacatc ctctcttgga gcgggcttgc cgtttggggc gcggtgttgg
    14401 ctgctgcctt tgccatcctg acccgactgc aaaccgccct gctgcttttt ttattgtccg
    14461 cccttgccgc caaccaattt ttggttaccc ccgttatcga ggcactgaaa tacgggcatg
    14521 aaaattggct gttgtcgttt gtaggcggat ccttcggaat gtggcacggt atttccagca
    14581 tgattttcat ggcaaccgcc ctactttcag cagttttaag ttggcggctt tccggcaaag
    14641 atgccgtctg aagccctccc atttttttac ctcccttcac ttcacttgga gaacattcat
    14701 gagcgcaatc gttgatattt tcgcccgcga aattttggac tcacgcggca accccacagt
    14761 cgagtgtgat gtattgctcg aatccggcgt aatgggacgc gcagccgtac cgagcggcgc
    14821 gtccaccggt caaaaagagg ctttggaact tcgcgacggc aacaaatccc gctattccgg
    14881 caaaggcgta ttgaaggcgg tcgaacacgt caacaaccaa atcgcccaag ccctcatcgg
    14941 tatcgatgcc aacgagcaat cttatatcga ccaaatcatg atcgaattgg acggtactga
    15001 aaacaaaggc aatttgggtg cgaatgcgac tttggcggtt tctatggcgg ttgcacgcgc
    15061 cgctgccgaa gactcaggcc tgccgcttta ccgctacttg ggcggcgcag gcccgatgtc
    15121 cctgcccgta ccgatgatga acgtcatcaa cggcggcgaa cacgccaaca acagcctgaa
    15181 catccaagag tttatgatta tgcccgtcgg cgcaaaatct ttccgcgaag cgttgcgctg
    15241 cggtgcggaa attttccacg ccttgaaaaa actgtgcgac agcaaaggct tcccgaccac
    15301 agtcggcgac gaaggcggtt tcgcccccaa cctgaacagc cacaaagaag ccctgcaact
    15361 gatggtcgag gcgaccgaag ccgccggcta caaagcgggc gaagacgtat tattcgcatt
    15421 ggactgcgcg tccagcgagt tctacaaaga cggcaaatac cacttggaag ccgaaggccg
    15481 ctcctacacc aacgcggaat ttgccgaata tctggaaagt ctggtcaacg agttccccat
    15541 catctccatc gaagacggca tggatgaaaa tgactgggaa ggctggaaac tgctgaccga
    15601 aaaactgggt ggcaaagtcc aactcgttgg cgacgacctc ttcgttacca acccgaaaat
    15661 ccttgccgaa ggcattgaaa aaggcgtggc aaacgcacta ttggtcaaag tcaaccaaat
    15721 cggtactttg agcgagaccc tgaaagccgt cgacttagcc aaacgcaacc gctacgccag
    15781 cgtaatgagc caccgctccg gcgaaaccga agacagcacc attgccgact tggcagtcgc
    15841 caccaactgt atgcagatca aaaccggttc tttgagccgt tccgaccgca tggcaaaata
    15901 caaccaactg ctgcgtatcg aggaagaatt ggcggaagcc gccgactacc ccggcaaagc
    15961 cgcattctac caactgggca aataagaaaa ggttaaggta tgaagtgggt aactgtcgtt
    16021 ttatccttcg cacttgtctg ttgccaatac agcctctggt tcggcaaagg cagcatcgga
    16081 cgcaacagca gtctgagaga acagattgcc gttcaagaag aaaaaaacca gacactcgcc
    16141 ctacgcaatc attcccttgc cgccgaagtc tatgatttgg aaaacggtca agaagccatt
    16201 tcggaaatcg cccgggtaga actgggttat atccaagacg gtgaaacctt ttaccgactc
    16261 atcaggcata accggtaata ccgtcaaaaa gccgtccgaa ccaatgttcg gacggctttt
    16321 atttcaacaa actgtcagac agcccctcat cctcccccga caaaccgcaa tccagcctga
    16381 catccccctc gacgcaacag cagcacggca gtatctcgtc ccgccccaaa aaagccaaag
    16441 gcggctcccg ataagtaacg cttccctcca aaatcttcac tcggcacgat ccgcaatatc
    16501 cgcttcggca ctgatattcc accatatgcc ccgtccgttc caagccttcc aacagggtct
    16561 cgccctccaa gagctcaaaa aaacccttat tcgtaccaat gcgcgccatt tccgaccaat
    16621 caaaaatata gtggattaaa tttaaaccag tacggcgttg cctcgccttg tccggatttt
    16681 tgttaatcca ctataatcca ctataaaagg aacaataacc gatcctaccc gctgtttttc
    16741 ccatcataca acatacaaat gccgtctgaa acatccggct tcagacggca ttttttcaaa
    16801 aaacatttac aactcaaaat cgcccaaatc atccgtattc acttcagaat cgatctgacc
    16861 gatcaaataa gaggatattt ccacttcctg cggcgcgacc tgtacgttgt cggacgacag
    16921 ccacgcatta atccacggaa tcgggttttg atttgcgcct tcaaatccgg ccggcaaccc
    16981 caccgcctgc atacgcagat tggtaatata ttcgacgtat tgggataaga tttctttgtt
    17041 caaaccaatc atcgaaccgt ctttaaacaa atatgccgcc cattcttttt cctgttccgc
    17101 cgcttttttg aagagttgga aacattcgtc ctgcaactca gcggcaattt ctgccatttc
    17161 agaatcatcg acacccgaac gcatcagatt aagcatatgc tgcgtgccgg tcaggtgcag
    17221 ggcttcgtcg cgggcaatca gtttgatgat tttggcgttg ccttccatca actcgcgctc
    17281 ggcaaaagca aacgagcagg cgaatgaaac gtagaaacgg atggcttcca acacgttgac
    17341 gcacatcagg cagagataga gttttttctt caacccgcgc aaagatacgg taacgggttt
    17401 tccgccgaca ttgtgcatcc cttcgcccaa caggttgtaa tactgggtgt attcgattaa
    17461 gtcatcgtaa tagcaggcaa tgtcttcggc gcgggcggta atgtattcgt tttcgacaat
    17521 atcatcaaac acgaccgacg gatcattcac aatattgcgg atgatgtggg tatagctgcg
    17581 cgagtgtatg gtttcgctga agctccacgt ttcaatccac gtttccaact cgggaatcga
    17641 aaccaaaggc agcaaggcaa cattcggact gcgcccttgg atggaatcga gcagtgtttg
    17701 gtatttcaga ttgctgatga aaatatgttt ttcgtgttca ggcaggttgg cgtagtcgat
    17761 acggtcgcgc gacacgtcga tttcttccgg ccgccaaaaa aacgacaatt gtttttcaat
    17821 cagtttttca aatacctcgt atttctgctg gtcataacgg gcgacattca caggctgacc
    17881 aaaaaacatc ggctcattca gcgcgtcgtt tttggttttg ggaaaggtgc tgtatgacat
    17941 gactaggtgt tcgcaggaca tggttttctc aggtttattt aaatttagga atttggattt
    18001 cagacagcct tatatgtttt aagtaaggtc atctgaatgt atctgtacat tgaattcttg
    18061 ccttaaatat cgctgttagt tataatcttc cccaacaact cccccaactt tgccaaccta
    18121 cgcggttgga gcaacaaggt tgggggcttt cttttatccc tgccacaatc tccagtctcg
    18181 cggattattt tccaaaccca tatcgcgcaa acttacagct tcattcgcga catcgggaaa
    18241 ctcatccagt aaagcaagaa gacggtttcc ccattcggtt cgcggacaaa taacatccaa
    18301 catccgtttc atcaggcaaa aatacacaaa cggtttattg ataggcaatg atttccattc
    18361 tgcatcagac aaacctttca agctggcttt aaaagtaata tccctattcc ataatcgact
    18421 gtgatgtgcc gaaacgttgc ggataaagtt aaatgcgtgc agatgtgttt gcagatgctt
    18481 accgtctttc aaacgataca atttagcaat agagtcttta tctttattca acatcccttt
    18541 atacagcata gacatcgtgc cgaaatccca tacttcgcag gctacccaga caggtaagtc
    18601 ggcataatgt tccaaatgat gtttaacaaa accggttttc ttttcgcgct cgaccagcct
    18661 gttataccta tccagccatt ccgcatggtc gaaacctgtg gcagaatatt tgctatcttt
    18721 gtaagccaat gggtcatgcc gtcctaatgt atgggcaata tttacccgaa gcgctacttc
    18781 aattctttcc agtgcatcta atgccaattg ccgcaatttc ttatcgaaca tataaaggct
    18841 tttgacatct tcaaaacgag tatctgtaat aaactcatcc aacctgctct tagaactttc
    18901 aggagcaaat tgtcggaaag agtaaaaata accgcttaat cggtaataac cgatggtgcg
    18961 taaatatcct aatgcctttt tctcatcatc aatctgcaat cctctacttc ttagaatctc
    19021 caactgctca tcgtaagatt tccaaagctt gaagcctttt atctgcttgc tttgcacagc
    19081 ctgtttcact ttcaaatagt taaatcttac aagccccgcc cgcgcagccg tcatcctgaa
    19141 tatcggtctg cgtatcgtcc gcgccgtcgc gggtgttatg gtagtacagg gttttgacgc
    19201 cgtatttgta ggcggtcagc aggtctttga gcatttgttt catagaaact ttgccgcctt
    19261 cgaatttgcc cgggtcgtag gcggtattgg cggaaatcga ttgatcgacg aatttttgca
    19321 tcacgccgac aagtttcagg tagccttcat tgccgggaag ctgccacagg gtttcatagg
    19381 catttttcag ggtttcaaac tccggcacga cttgtttcaa aatgccgtct ttcgatgctt
    19441 tgaccgttac caatccgcgc ggcggctcga taccgttggt ggcgttggcg atttgcgaac
    19501 tggtttcaga cggcatgagt gcggttagag tagagttgcg caggccgtat ttgacgattt
    19561 cggcacgcag gctttcccag tcgtaatgca aaggctcgcc gcagacggca tccaaatctt
    19621 ttttgtaggt gtcgatgggc agtttgcctt gcgaataaac ggtttggtta aagagcgtac
    19681 acgcaccgta ttctttggca aggtttgccg atgctttgag cagataatac tgtatggctt
    19741 caaaggtacg gtgggtcaga ccgagcgcgg agccgtcgct gtagcggaca ccgtttttcg
    19801 ccagataata agcatagttg atgacgccga tgccgagcga acggcgaccc atagtagagg
    19861 tacgcgcggc ttctaccgga tatccctgat aatctaaaag tgcatcgagc gcgcgaacgg
    19921 tcaagtcggc aagcccttcc aattcgtcca agctgtttaa tgcgcccaag ttaaaggcag
    19981 acagtgtaca caaggcgatt tcgccgttcg gatcgttgat attgtccagc ggtttggtcg
    20041 gcagggcgat ttccatacac aagttggact gatgaacagg cgcgacgcgc ggatcgaacg
    20101 ggctgtgcgt attgcagtga tcgacgtttt gaatgtagat gcgcccggtt ccggcacgct
    20161 cctgcatcag cgtggaaaac aggtcggcag ccggaatgat gcgcttgcgg atatcagggg
    20221 cttgctcgta tttcgtatag agccgctcaa attcgtcttg gtcggcaaaa aacgcttcgt
    20281 acaatcccgg aacctcgttg ggcgaaaaca gcgtaatgtt gccgccctta atcaggcggg
    20341 tgtacagcag gcggttgatt tgcacgccgt aatcaagctg acggatacgg ttgtcttcca
    20401 caccgcggtt gtttttcaac accagcaggc tttcggcttc gatatgccac aaagggtaga
    20461 acaaggttgc cgcgccgccg cgcacgccac cttgcgaaca ggatttgacc gccgcctgaa
    20521 acattttaaa gaagggaatg cagccggtat gccgcgcttc gccgccccgg atttcgctgt
    20581 ccaaaccgcg gatacgtccg gcattgatgc cgatgcccgc acgctgggaa acgtatttca
    20641 caatcgcgct ggtagtggca ttgatggaat ccaaactatc gtcgcattca atcagcacac
    20701 agcttgagaa ctggcgcgta ggcgtacgca cgccgctcat aatcggggtc ggcagcgata
    20761 ctttaaatgt ggaaacggca tcgtaaaacc gtttgacgta atcgaggcgt gtttctttgg
    20821 gatatttgct gaaaagacac atcgccacca aaacatataa gaactgcggt gtttcataaa
    20881 tctggcgggt aacgcggttc tgtaccagat atttgccttc gagctgtttg acagcggcat
    20941 aggaaaagga catatcgcgt tcgtggtcga tataggcgtt cagctcgtca aattcttcgc
    21001 ggctgtaatc ctcaaggata tgcctgtcgt attttccagc atcggtaagt tttttaacgt
    21061 ggtcgtaaag gtgcggcggc tcgtactcgc cgtaggctat tttacgaaga tggaaaatgg
    21121 ccaaccgcgc ggcaaggtat tggtagtccg gggtatcttc cgaaattaaa tcggcagcgg
    21181 ctttgatgat ggtttcgtgg atgtcgtcgg tgcggatgcc gttgtagaac tggatgtgcg
    21241 atttcaactc gacctgcgac acggaaacat tttccaatcc gtccgccgcc caagtgacga
    21301 cacggtgaat cttatccaaa tcaatggctt ctaatcttcc gtctcgtttg gttactttca
    21361 aatcagtcgg tgtattcatc gcttcctctt ccactcttga tattcaagac acggtctttt
    21421 caaataaatt aagacagaca atatagtgga tttttggcat tttctccagt cttgacaacg
    21481 gttgcatttt tcagtttcag gcaatgcgcg ataaaacccg cctgtcgtat ttttcctata
    21541 atatttgttt tatctgataa ttctttaccg ataaaaaacg ggtaaatttt ttgccttttg
    21601 accggctccg gctacaaggc ggtgaaataa ggattttccg acgaaaaagg aaagcttccg
    21661 gttttctgcc cctgcgaatt gttaaatttt gcaaagtatg attttgccgc gccgccgccg
    21721 acaaattcca ttttcttacc gattgaaatt tattattg
//
LOCUS       DAXIEB010000022        21243 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000022 DAXIEB010000000
VERSION     DAXIEB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.022"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            295..1728
                     /locus_tag="ACFOA3_000713"
     CDS             295..1728
                     /locus_tag="ACFOA3_000713"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6897752.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAVLKLGNGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIAVANDTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEVAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCLDVG"
     gene            1972..2760
                     /locus_tag="ACFOA3_000714"
     CDS             1972..2760
                     /locus_tag="ACFOA3_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6897753.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVIEAFEKDRINIVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3128..4684
                     /locus_tag="ACFOA3_000715"
     CDS             3128..4684
                     /locus_tag="ACFOA3_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6897754.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4701..5447
                     /gene="surE"
                     /locus_tag="ACFOA3_000716"
     CDS             4701..5447
                     /gene="surE"
                     /locus_tag="ACFOA3_000716"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6897755.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            5703..6926
                     /locus_tag="ACFOA3_000717"
     CDS             5703..6926
                     /locus_tag="ACFOA3_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6897756.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPASDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKLAGYAAPKTAAVESRPAVPAAAQPPVQSAPQPAAPAAENKAVPAPAPQS
                     PAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGS
                     GLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEV
                     RQNGKPVNPNSYIAF"
     gene            complement(7133..7615)
                     /locus_tag="ACFOA3_000718"
     CDS             complement(7133..7615)
                     /locus_tag="ACFOA3_000718"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6897757.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EVRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(7768..7929)
                     /locus_tag="ACFOA3_000719"
     CDS             complement(7768..7929)
                     /locus_tag="ACFOA3_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897758.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(8003..8464)
                     /gene="recX"
                     /locus_tag="ACFOA3_000720"
     CDS             complement(8003..8464)
                     /gene="recX"
                     /locus_tag="ACFOA3_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6897759.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPYAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(8537..9247)
                     /locus_tag="ACFOA3_000721"
     CDS             complement(8537..9247)
                     /locus_tag="ACFOA3_000721"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6897760.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            9654..10919
                     /locus_tag="ACFOA3_000722"
     CDS             9654..10919
                     /locus_tag="ACFOA3_000722"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6897761.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(10977..11783)
                     /locus_tag="ACFOA3_000723"
     CDS             complement(10977..11783)
                     /locus_tag="ACFOA3_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6897762.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            12128..12337
                     /locus_tag="ACFOA3_000724"
     CDS             12128..12337
                     /locus_tag="ACFOA3_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6897763.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            12426..13640
                     /locus_tag="ACFOA3_000725"
     CDS             12426..13640
                     /locus_tag="ACFOA3_000725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6897764.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(13727..16306)
                     /gene="clpB"
                     /locus_tag="ACFOA3_000726"
     CDS             complement(13727..16306)
                     /gene="clpB"
                     /locus_tag="ACFOA3_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6897765.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNMPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGTANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            16567..17577
                     /gene="trpS"
                     /locus_tag="ACFOA3_000727"
     CDS             16567..17577
                     /gene="trpS"
                     /locus_tag="ACFOA3_000727"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6897766.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            17583..17924
                     /locus_tag="ACFOA3_000728"
     CDS             17583..17924
                     /locus_tag="ACFOA3_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897767.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPGDLEAVSGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            18245..18568
                     /locus_tag="ACFOA3_000729"
     CDS             18245..18568
                     /locus_tag="ACFOA3_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897768.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(18777..20285)
                     /gene="ppx"
                     /locus_tag="ACFOA3_000730"
     CDS             complement(18777..20285)
                     /gene="ppx"
                     /locus_tag="ACFOA3_000730"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6897769.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKIIAKDFQAAISAARNEIQRISKNMRREGWDSAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(20401..21243)
                     /locus_tag="ACFOA3_000731"
                     /pseudo
     CDS             complement(20401..21243)
                     /locus_tag="ACFOA3_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714481.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         4832 a         5352 c         5843 g         5216 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tattaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cgctgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattccc aattaatcaa gcatcccgat atttcccttc ccccggtttc agacggcatc
      361 aaagtcgata atccggcaac gggcgagact ttggcgtttg tccgcaagac ggattcggac
      421 aagctgaaaa acctgattca aaaggcggcg gcagcgcaaa aattatgggc ggcaaaaacc
      481 gcgttggaac gcgccgatgt gttgtggcgt tggtattttt tgataaagga aaacaaagaa
      541 gcactggcgc gtctcatgac gatggagcag ggcaaaggcc tgaccgaggc gcgcggcgaa
      601 atcgattatg cggcttcgtt tgtgcgctgg tttgccgaag aggcgcggcg gattgacggc
      661 gatgtgctga cgagtgtgaa agcgtcgcaa aaactggtcg tgttgaaaca gcctgtcggt
      721 gttaccgccg cgattacgcc gtggaacttt ccgtccgcga tgattgcgcg caaggctgcg
      781 ccagctttgg cagtgggttg cgcgatgatc gtcaaacccg cgtcgctcac gcctttgagc
      841 gcgtatgcgt tggccgtgtt ggcttacgaa gcgggcgtgc cgcaggattt gctgcctgtt
      901 gtcagcggca gtgcttcgga aatcggccgt gaatttgcca cgaacccgat tatccgcaaa
      961 atcagcttca ccggttcgac cgaagtcggc gcgaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga actgggcggc aatgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggcgcgctc gccagcaagt tccgcaacag cggtcagacc
     1141 tgtgtctgca ccaaccgcgt ttacgctcaa tcgggcattt acgacgcatt ttgccgcaaa
     1201 ttaagtgaaa aagcagccgt gctcaaattg ggcaacggct tggacgaagg cgtgaaccaa
     1261 gggccgctga ttgaggaaaa agcggtagaa aaagtcgagc agcacatcgc cgacgcactc
     1321 tcaaaaggcg cggtctgtct gaccggcggc aaacgcagcg cgttgggcgg aacgtttttt
     1381 gagccgactg tcttaagcgg cgtaacagca caaatggcgg tggcgcgcga ggaaaccttc
     1441 gggccgttgt gtccagtgtt ccgttttgaa accgaagccg aagtcatcgc ggtcgccaac
     1501 gatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag cattggaata cggtatggtc ggcatcaata cgggcttaat cagtaatgaa
     1621 gtggcaccgt tcggcggcgt gaaacgcagc ggtttgggac gtgaaggcag caaatatggt
     1681 gcggacgaat atctagaatt gaaatatttg tgtcttgatg tcgggtagag tatatatttg
     1741 atttatatgc aaaaagtttt tgggtatgta aatctgatag attcacatac cccaacttat
     1801 accccaattt atttgaagtt ggtttgaagc cagtttgcta cggcttcttc gttccaccgg
     1861 ctgctcctgc cgtctgaacg gcagggtttc agacggcgcc gcattttaag cagtctctat
     1921 ctgttgtaca atgcgccctg tttttacggt tatttttgat ttgaacaaga tatgatggaa
     1981 aacggaaaaa cagtcccgag atggcgtttt gccttgaaaa gtgcgggctg gcacctctta
     2041 atcagcctgt cggttgcagg gctggcggca ttgctggttt ttaaggtttg gtatccttat
     2101 ccttatgccg agctgacggg agggctgtcg ctttatcagc tggtggtggc tgtcgatatt
     2161 gtatgcggtc cgctgctgac tttaattttg gcaagcccga agaaaaagac aaaggcacgc
     2221 atggtcgatt tttccatggt cggcatcatc cagctggcgg ctttggcgta cggtctgcac
     2281 agcgtttcgc tggcgcgccc tgtgatagag gcgtttgaaa aagacaggat taatattgta
     2341 accgccgccg aagtggcggt tgaagatttg cacaaagcgc cggaagggct gcaaagcctg
     2401 tcgtggttcg gcatccgccg cattgcattg aaagaacctg aggatgcgga tgagaagaac
     2461 aagacgctgg atttgtccct gaaaggtatc gagccgagta tgcgtcccga ccggtggctg
     2521 ccgtattccg acaaggaagc agaagaaatc cgcaaacatt tgaaaccgct gaaagtcttg
     2581 gcggatgcga gaaaaacgac ggttgcggac attctgaaac aggcaggtct cgccgaaggg
     2641 gaggagctgt attacctgcc gtttaccagc agcaggcaga aagagtggat agtcattacc
     2701 gataaagagg gcaacaccaa aggctacgcg ccgatagacg gcttcatcat caccccttaa
     2761 gcgttgggac tccgtccgca ctcgaacatc cgttcttcgc ggcggtagaa tcagactgta
     2821 tttgagaggg gcgcaacccc tcaatcaggg caacacatag gggaaggctt tatgtgccgt
     2881 ccgtgtgttg aaacatttca aaataagctc atgccgtccg aacatccttt cagacggtat
     2941 ggcgttttgc cattgccccg ataagctgtt aaactattta aattatttcg accgaaggta
     3001 cacgcacatg caagaccccg agcagagcag caagcccgcc cgctgttttt tgtgcgtacc
     3061 gtctgttcag gcagcttgag cgcttcaggc tgccttttcg cgtttgaact tgaggaatac
     3121 gaaaatcatg gatttcagtt ggttggcaga accgcatacc tggataggtt ttgccacgct
     3181 tttggtgttg gaagtcgtat tggggataga caatcttgtc tttgtggcga ttttggcaaa
     3241 caaggtccag cccgcacggc gcgaccgcgc acggattatc gggctggggc tggcagtcgt
     3301 catccgcatc attatgcttg cttttatggc gcacatcatc acgctgaccg agccgctgtt
     3361 ccaaatcggc ggcctcgccg tttccggcaa ggacatgatt atgctcgcgg gcggtatttt
     3421 cctgctttac aaagccacca ccgaactgca tgaacgcctc gaagggcaca accgttttac
     3481 cgttgccgac aaccaaaaaa aacacgcgcc gttttggggc gtggtcgcgc aaatcctgat
     3541 actggatgcc gtgttttcca tcgattcggt cattactgcg gtggcgatgg tcgatcatat
     3601 cgtcgtggcg atgggtgcgg tcgtcgtcgc gatggctgta atgatttctg ccagcaaact
     3661 cttgaccgaa tttgtcgaca gacaccctac cgtcgtgatg ctctgccttg gttttttgtt
     3721 gatgatcggt ttcagcctga ttgccgaagc cttccatttc cacattccca aaggctacct
     3781 ctacgccgcc atcggcttct cgattttaat cgaactgttt aaccagattt cccagcgcaa
     3841 cagccgcaaa aacgactaca tcggcagctc gtggcgcaag cgcaccgccg aaaacgtttt
     3901 gggtatgatg ggcatacgcg aaagcgtgct tgccgatgcg ggcggcgaat ctggggacga
     3961 cgcgcatttt gaagaaaacg aaaaatcgat gatacgcagc gtgctgacgc ttgccgaacg
     4021 cccgattatg ggggtgatga tcccacgccg cgacatcgaa cggctggaca tttcccaaag
     4081 ccgcgaagaa cagtgtgccc aactgcaaaa tacgccttac agccgcctgc tcgttgtcgg
     4141 taaggcgggc gtggacgaac ctttgggcta catcaacaaa aaagacctgc tgtcccaact
     4201 gctggaaaca ggcggtctcg acattcagac ggcattgcgc cagccactcg tcctgcccga
     4261 cagcaccacc gcgctgggcg caatcgaact cttccgccaa agcagcgcgg attatgcttt
     4321 ggtggtggac gagttcggcg cggtattggg catggtaacc atgaaagacc tgctcgaaac
     4381 catcgcaggc gagttccccg aagaatttga gcgcgaagaa gaaccagccg ttcaggggaa
     4441 tcccgatgaa agcctgacgg tggaaggcgc gttggaatat gtggaactcg caccgcaact
     4501 caacctgccg cagcagcagg aagatgccga tttccatacg gttgccgggc tgattatgga
     4561 agaattgcaa accatccccg atgtcggcga ttttgccgat ttccacggct ggcggtttga
     4621 agtggtcgaa aaagaagggc agcgcatcga gcgggtcaaa atcaccaaat tgcccgaaga
     4681 ataagcattc aggatagaaa atgaacgttt tgatttccaa cgacgacggc tacctctccg
     4741 aaggcatcgc cgttttggcg cgcgttacgg cggaatttgc caacgtcagg gtggtcgcgc
     4801 ccgaacgcga caggagcggg gtcagcaatt cgctgacact ggaacgccct ttgcagttga
     4861 aacaggcgca aaacggcttt tactatgtca acggcacgcc gaccgactgc atccacatcg
     4921 ggcagtctgt attttcggat tttcaggccg attttgtctt ttcgggcatc aaccggggcg
     4981 cgaatatggg ggacgacacg ctttattcgg ggacggttgc ggcggcaacc gaagcctacc
     5041 ttatgggcat acccgctgtg gcgttttcct taaacgacgc ttccggacgc tattgggcga
     5101 ccgcagaaca ggcactgtgg acattgttgg cgcatttttt caaaaacccc ccgcagtccc
     5161 ctattttgtg gaacatcaat atccccgccg ttgcgccgga agatgtgcgg ggcattaaaa
     5221 tcgcccgttt gggcaggagg catcacggtc agaacgtcat tcccgcgcgc aatccgcgcg
     5281 gcgaacagat ttattggata ggaccggtcg gcgaagtttc cgatcgggaa gagggaacgg
     5341 atttcggtga atgcggcgca ggtttcatta ccgtaacgcc gctgcaaatc gacctgaccg
     5401 cctatccgga catggcggaa acagcggcgt tctggcatgc ggactgaccg tttcatcaaa
     5461 tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     5521 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
     5581 cgtcgccttg tcctgatttt tgttaatcca ctataccgtc gggctttgcg cttgagaaga
     5641 acgcgcattt ttattatgat ataccgcatt caggctatat atcggaacat gaaggttatc
     5701 ttatgttgaa acaaacgaca cttttggcgg cttgcgcctc ctttgccgca ctgttgggcg
     5761 gttgtgctac ccagcagcct gcccccgtca ttgcaggcaa ttcaggtatg caggacgcgc
     5821 cgtcttcggc agtttacaac aacccctatg gagcaacgcc gtacagcccg gctcctgcca
     5881 gcgatgcgcc ttatgtgccg ccggtgcaaa gcgcgccggt ttatacgcct cctgcttatg
     5941 ttccgccgtc tgcacctgcc gtttcgggta catacattcc ttcttacgca cccgtcgaca
     6001 tcaacgcggc gacgcatact attgtgcgcg gcgacacggt gtacaacatt tccaaacgct
     6061 accatatctc tcaagacgat ttccgtgcgt ggaacggcat gaccgacaat acgttgagca
     6121 tcggtcagat tgttaaagtc aaaccggcag gatatgccgc accgaaagcc gcagccgtaa
     6181 aaagcaggcc cgccgtaccg gctgccgcgc aaccgcccgt acagtccgca cccgtcgaca
     6241 tcaacgcggc gacgcatact attgtgcgcg gcgacacggt gtacaacatt tccaaacgct
     6301 accatatctc tcaagacgat ttccgtgcgt ggaacggcat gaccgacaat atgttgagca
     6361 tcggtcagat tgttaaagtc aaactggcag gatatgccgc accgaaaacc gcagccgtag
     6421 aaagcaggcc cgccgtaccg gccgccgcgc aaccgcctgt gcagtccgcg ccgcaacctg
     6481 ccgcgcccgc tgcggaaaat aaagcggttc ccgcgcccgc cccgcaatct cctgccgctt
     6541 cgccttccgg cacgcgttcg gtcggcggca ttgtttggca gcgtccgacg caaggtaaag
     6601 tggttgccga tttcggcggc aacaacaagg gtgtcgatat tgccggtaat gcgggacagc
     6661 ccgttttggc ggcggctgac ggcaaagtgg tttatgcagg ttccggtttg aggggatacg
     6721 gcaatctggt catcatccag cataattctt ccttcctgac cgcatacggg cacaaccaaa
     6781 aattgctggt cggcgaaggc cagcaggtca aacgcgggca gcaggtcgct ttgatgggca
     6841 ataccgacgc ttccagaacg cagcttcatt tcgaggtgcg gcaaaacggc aaaccggtta
     6901 atccgaacag ctatatcgcg ttctgacttt ctgcccgaaa tcaagttgga aatgccgtct
     6961 gaaaggtttt cagacggcat ttttgttttt ggcggctgtg ttttcaaatt attcggaagg
     7021 gacggggcat ataaatttgt ttgactgtgt ttgggtattt gcttgatttc atccgtttgc
     7081 agtagaaacg gatggcgcga tgtgcctgtc tttcagacgg catagtccgc cgtcagcagc
     7141 cgcaggacat gtcttcagag gcttgcaggc tgatgtctct gcgttttttg gctttttcgt
     7201 agcggcagcg ttggcggtcg gtcaggattt ccagcggagg gacagggacg ggtttgccgt
     7261 ctttgaccgc aaccatcgtg aagtagcaac tgttggtatg gcggacttcc cccgtgcgga
     7321 tgttttgtgc ttcgacacgg atgccgattt ccatcgaggt gcgtcccgtg tagttgacag
     7381 cggcgtaaaa ggtaaccaaa tcgccgatgt ggatgggttc tttaaacagg actttgtcaa
     7441 ccgacagggt aacgcaataa ttgccgctgt aacggctggc gcaggaatag gcgacttggt
     7501 cgagcaggag caggagttcg ccgccgtgta cgttgccgct gaaattggcg gtgtccggca
     7561 tcatcagttc ggacataatg agttcgtggg aaggcagttg gcgttgttgt gtcatagtgt
     7621 acctgtttta agaaagggtg ttttgaaatg ggtgtgggaa aattttaggg aatggggaag
     7681 tggaattcaa gctaaaaaaa gctatggcgg gatagtctgc agaaatgggc cgccgatgcc
     7741 gtctgaagcc ttcagacggc attttgttta ggaaagccaa aaacggaaga ccaggaaaaa
     7801 cacgagtgcc aatacgcctg cccaaacgac gagcatgaat ccggcaactg cctgccccat
     7861 gcggtacacg gggttcttgc gttcgtcctc aaggaggggg ttatgatttt tttcgggttg
     7921 tccggacatt ttgttctcaa tcggaaaagt tggaaacggg cgtaaggtta tgttgccgtg
     7981 caaaaagatt caagaaggaa ggtcagcagc cgtcttccca attttcgtcc caagcgtgtt
     8041 tcaatgccgt ctgaacggta tcggcatcga atccgcgata ggcgaggaaa cgcgcctgtt
     8101 tttgtttttc tttgaggtcg gcggcggggt gtttgaattt tttacgcaac acggctatgg
     8161 cggcctgttt ttcgcttgag cggtcgggaa gcaggttgcg gctggtttct tcatctatgc
     8221 cctgttgcgc caaagcctgt ttcagcctca atgaaccgtg tttgcggctt ttgctgcgga
     8281 tataggcttc ggcatagcgc aaatccgact gccagttgcg ttcggcaaat tcgtttaaca
     8341 cgttttccaa ctcctcttcg ctttcggcgt acggtgcaag tttgcgtttc agaccgatgc
     8401 ggctgacttc ttggcgcgag aggatgtcca tcgcgcgggc gcgtagggat ttttgcggtt
     8461 tcatggcggc ggtttcggca cggaaagatt cagacggcat gggaaacgga ttccgatgcc
     8521 gtctgaacag attgttttat tcttcgtctt tatttttctg cggttgcagg ttttcgtaaa
     8581 tttcgggcag cgagccgata atccagtcgg ggcgggtcgc atcgtcttgc gagagcagcg
     8641 tcatatcgcc gtaaccgaag gtaacgccga cgctcaggca gccggcggct ttggcggcga
     8701 tgatgtcgtt gcgcgagtcg ccgaccataa ccatgtttgc aacatcgata cccaaaactt
     8761 cggcggcatg ccgcagcggc agggggctgg gttttttctc gggcaggctg tcgccgccga
     8821 gtatcaggct gaagtagtcg gcaagtccga gctgtttcaa cagttcgacg gcaaggattt
     8881 cgtttttgtt ggtaatgact gccagcggga tgcccaaaga tttgagcaat gccagcccgg
     8941 cttcggtttc gggatagggg cgggtgaaga cgctcaaatg gtcgcggtag tatttcatat
     9001 aggacacgaa acctttttcc cacagttcgg aatcggcttc gcggtcgcgg tcgttggtga
     9061 ggacgcggtg aaccagtttg ccgatgccgt cgcccacata gctttcgacc actttggcgg
     9121 gcagcggttt cataccgagt tgctccaaca tcgcttctgc ggcggcggca aggtcgggga
     9181 cggaatcgca cagtgtgccg tccaaatcga aggcgacggc ttggacgtgt tcgatggctg
     9241 cattcataat ctgatttcct gtcgggttga aagggtttat tttaacatcg tttgcacagg
     9301 gcttctaatg tatagattgt taacaatcgc aacaggtttt gcaggcggtg ttttcggatt
     9361 tcggggcgga atcgggcaag ggtttgaaat cccggcgatg ttccaattaa atttttaaaa
     9421 aataaaagga tgcctatatc tttatttaat ttgacattta tttttcggac gcgcgcgctg
     9481 ctttttcagg cggagtccgg gctgattccg tgcggcttga aaaataaaat catatataga
     9541 ttaatcaaat cgggcttatg tcgactacac gctattacat tttgtcggga aattggttat
     9601 agtaatgagc gaaatgcacg cccctgaaaa tctttttgtg aaaaggaagc aaaatgtctg
     9661 aagcccttgc aaaagaaacc ctcaatccgt ttgaaatcgc gcgcaaacag gtcaaaaccg
     9721 cctgcgaccg gctgaaaacc gatccggccg tttatgaaat cctgaagagt ccgacccgcg
     9781 tgttggaagt caatttcccc gtcaaactcg acgacggcac ggtcaaaacc ttcacgggct
     9841 accgttcgca acacaacaac gccgtcggcc cctataaagg cggcgtgcgc ttccatcccg
     9901 gtgtgaatct ggacgaagtc aaagccctgt cgatttggat gaccatcaaa tgctgcgtgg
     9961 ccggcattcc ttacggcggc ggcaaaggcg gtattacctt ggatccgcgc gattattccg
    10021 aggcggaact ggaacgcatc gctcgcgctt attccgaagc catttctccg ctgattggcg
    10081 agaaaatcga tattcccgcc cccgatgtga ataccaacgg caaaattatg tcgtggatgg
    10141 tggatgctta tgaaaacgtg gtgaaacatt ccgcgccggg cgtatttacc ggaaaaccgg
    10201 ttgagttcgg cggctctttg gcgcgtaccg aggctaccgg ttacggcgtg aacctcgccg
    10261 ccgtccaagc tttggaaaaa ctgggcaagg acgtgaaggg tgccacttac gccattcaag
    10321 gtttcggtaa tgtcggctac cacacaggtt attacgcgca tcaatccggt gcgaaaatcg
    10381 ttgccgtttc tactgttgac gttgccatct acaacgaaaa cggtttggat atggaagctt
    10441 tgtttaaaga gttccaagaa aaaggcttca tcaccaatga agccggctac ggtaaagaaa
    10501 tcagcaatgc cgaacttttg gctctggaag tggacgtact tgccccttgc gcactggaaa
    10561 accaactgac ttctgaaaat gccggtaaag tccgcgcgac aatcgtggtc gaaggcgcaa
    10621 atggcccgac tactccagag gcagacgtta tcctgcgtca aaacggcgta ttggtcgttc
    10681 ctgatatttt ggcaaactgc ggcggcgtgg tggtatccta ttttgaatgg gtacaaaacc
    10741 tgcaaggcta ctactgggag tttgacgaag ttcaagagaa agaaaccgtt gtcctgcgcc
    10801 gtgcgttccg cgatatatgg aacttggcgc aagagtatga tgtcgatttg cgaaccgcgt
    10861 cttacatgat gagtatccgc cgtgttgaaa aagcgatgaa actgcgcggt tggtattgat
    10921 gtttgaacaa aaaatgccgt ctgaagcctt cagacggcat ttttgtgcgg gcgggattaa
    10981 tggtgcgaat ggccgatttg gaaggttaaa gtcgagtagt tcgcctgttt ttggcacacg
    11041 ctttgatcgg ggaagtcggc tttgtgttcg acattggctt tccagaagcc ttggcgcaag
    11101 gggatgatgt ccacttcgcc tttgtcgtct gtgctgtcgg agaaagcctg tgcttcggtt
    11161 ttgtgcgttt tgctgcggtc gctggtgtcg aagccgtcaa aggtggcggt aacggtggca
    11221 ttgggcagcg gttcgccacg gaacagaacg cggactttga agcgttcgcc tacgtgaatg
    11281 ttggcgggat tgtccagcgg gacgatttcc aagttttgtc cgaccggttt ggtgatgatg
    11341 gcggtgtccg cgctttcgtg tccgacgttg acgatgtttt tgccgaacat tcgggtttgt
    11401 tcgcaatagc ttgcgtcagg catttctttg atgcccgcct gtttccagcc tgctttgttt
    11461 tttgaccaga aagtaggctg atattcggcg atgacgaggt aactgccgtc cttaacggga
    11521 cggttgcttc ggtactggta gttgtatgtg ccgcgttgaa tcatgttttc cttgcctttt
    11581 tcggtaacca gctgcatcgg tttgctgaag atgtgcaggc ggtctttggc gatgggttcg
    11641 agttcgggaa attcgccgta gcccaagtcg gctttaaggt attcgccgcc gtgcgtgtgg
    11701 gcggtttcga cccagacgcg gtgggcgtgg gcggatgtgg caaatagggc ggaaacggcg
    11761 agcagtgtca atgttttctt catggtgcag cctttgtctc gtgtgggtta aagattttgt
    11821 tatgttataa caaaataggt atcgtgtgaa gacggatttg ctgtctgaaa actgtctttt
    11881 tgtatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga
    11941 ctctctaagg tgctgaagca ccaagtgaat cggttccgta ctatctgtac tgtctgcggc
    12001 ttcgtcgcct tgtcctgatt tttgttaatc cactatatcg atttgaattt ttcagaaaat
    12061 gaagcggacg tttgggcgga ggcaacttgt ttgataagat agcaatattt caaaacggag
    12121 aaagatcatg ccttatgtca atattaaagt aaccggcggc aaggaagcac cgactgccgc
    12181 gcaaaaagcg gaactgatcg gcggcgtaac cgaattgctg gcacgcgtgc tgggcaaaaa
    12241 tcccgaaaca acggttgtcg tgattgacga agtggatacc gataactggg gaataggcgg
    12301 caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa gcctgaaaat ctcggtttga
    12361 tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca ttgcccaaaa tttacggaac
    12421 acattatgga taaatttccc aagtctgcaa agctcgatca cgtctgttac gacatacgcg
    12481 ggccggttca caaaaaagcc cttcagttgg aagaggaggg caataaaatc cttaaactca
    12541 atatcggcaa ccctgcgccg ttcggctttg aagcccctga tgaaatcttg gtcgatgtca
    12601 tccgcaacct gccgacttcg caaggctatt gcgattctaa agggctgtat tccgcccgca
    12661 aagccattgt tcactactat cagaccaaag gtttgcgcga tattacggta aacgatgtct
    12721 atatcggcaa cggcgtgtcc gagctgatta cgatgtctat gcaggcattg ctcaacgacg
    12781 gcgacgaaat cctgattccc gcgcccgact accctttgtg gacggcggcg gcaacgcttg
    12841 cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa cggctggttc cccaaccttg
    12901 ccgatatgga agccaaaatc acgcccaaaa ccaaagccat cgtcgtcatc aatcccaata
    12961 atccgacagg cgcggtgtac agcagggaaa tcctgttgga aatcgccgaa ctggcgcgca
    13021 agcacggttt gattattttc gccgacgaga tttacgataa aatcctttat gacggcgcgg
    13081 ttcaccacca tattgccgcg cttgcccccg accttttgac ggtaacgttc aacggtttgt
    13141 ccaaagccta ccgcgtcgcc ggattccgcc aaggctggat ggtgctcaac gggccgaaac
    13201 atcatgcaaa aggttacatc gagggtttgg atatgctctc gtctatgcgc ctgtgtgcca
    13261 atacgccgat gcagcacgcg attcagacgg cattgggcgg ctatcagagc attaacgaat
    13321 ttatcctgcc gggcgggcgg cttttggaac agcgcaacag ggcgtgggaa ctggtcagcc
    13381 aaatccccgg cgtgtcctgc gtcaaaccga tgggcgcgat gtatatgttc ccgaaaatcg
    13441 ataccgaaat gtaccgcatc cgcgatgaca tgaaattcgt ttacgatttg ctggtgcgcg
    13501 aaaaagtctt gctggtacag ggaacggggt ttaattggat caagcccgac catttccgca
    13561 ttgtcacgct gccttacgtc catcagattg aagaggcgat gggcaggctg gcaagattcc
    13621 tgcaaaccta ccgccaataa ggggacggtt tgtctgccga ggataaaaaa tgccgtctga
    13681 aacggagatt cccgtttcag acggcatttc caacagcagg aacaaattag gcaaatttca
    13741 gtctgtcgcc gtcggcttcc accctgattt cgctttcggg cgcatagttt ccggcaagca
    13801 gggcttttgc cagcgggttt tcgatttccg actggatggc gcgtttgagc ggacgtgcgc
    13861 cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag tgcggcatcg gaaacagcca
    13921 ggcgcaggtt ttgtttttcc aaacgttttt ccaagccttt gagctggatt ttcgcaatgt
    13981 tgcggatatt atcctgatcc agtccgtgga acacgaccac ttcgtcgata cggttgatca
    14041 tttcaggacg gaaatgttct ttcacatcct ccatcacaac ttctttcacc gcttcgtagt
    14101 cttgagtacc catttgttgg atatgttggc taccaatatt ggaagtcatc acgataacgg
    14161 tatttttgaa gtccacggtg cgaccttgtc cgtcggtcaa gcggccgtca tccaatactt
    14221 gcagcaggat gttgaacaca tcgggatggg ctttttccac ttcgtccagc agaatcacgc
    14281 tgtacggttt gcggcgcact tgttcggtca ggtagccgcc ttcttcgtag ccgacatagc
    14341 ccggaggcgc gccgattaag cgggcaacgg cgtgtttttc catatattcg gacatatcga
    14401 tgcgaatcag atgatcttcg ctgtcgaaca gaaagcctgc cagggcttta cacaactcgg
    14461 ttttacccac gccggtcggg cccaagaaca ggaagctgcc gtaaggcttg ttcggatcgg
    14521 caagaccgga gcggctgcgg cggatagcgt cggacacggc acgcacggct tcgtcctgtc
    14581 cgaccacgcg gcggtgcaat acttcttcca ttttcagcag tttgtcgcgt tcgccttcca
    14641 tcattttgga tacgggaatg ccggtcatac gggaaaccac ctctgcgatt tcctctgcgc
    14701 cgacattatt acgcaagagt ttgtttgccg gttttgtgct gtccgtatct gcccgttcgg
    14761 cggctgcacg ctgtttttcc aaatgctcca aatcttcata catcaatttt gaagccagtg
    14821 ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt gacttcgtca atttgtttct
    14881 taatattagc agtaccgtct gaaattgctt tttcggcttt ccagatttcg tctaaatcgg
    14941 cgtattcttt ttgcagaccg ttgatttcct cgtctatcag ttccaaacgt tttttgctgg
    15001 catcgtcttt ttctttttca acgtgcgcct tttccatccg aagctgaatt agacggcggt
    15061 cgattttgtc cattgcttcc ggcttggttt ctttttccat cttgacacgg ctggcggctt
    15121 cgtcaatcaa atcaatcgct ttatcgggca ggaagcggtc ggtaatgtag cggtcgctca
    15181 actccgctgc ggcaacgata gcagggtcgg taatatcgat accatggtgg atttcataac
    15241 gctcctgtaa accgcgcaaa atagcgatgg tgtcttccac gcttggctcg ccaaccaata
    15301 ctttttggaa gcggcgttcg agtgccgcat ctttttcgat gtattggcgg tattcgtcca
    15361 aagtggtcgc gccgatacag tgcaattcgc cacgtgccaa agccggtttc agcatattgc
    15421 ccgcatccat cgcaccgtcg gttttgcctg cgccgaccaa agtatggatt tcgtcgatga
    15481 aaatcagagt gttgccgtcg tctttcgcca aatcgttcaa cacgcctttc aagcgttctt
    15541 caaattcgcc gcggtatttc gcgccggcaa tcaaagccgc caagtccaga accaaaaggc
    15601 gtttgttacg cagggattca ggcacttcgc cgttgacgat acgttgcgcc aagccttcaa
    15661 caatagcggt tttacccaca cccggctcac caatcagcac agggttgttt ttggtacggc
    15721 gttgcaatac ctgaattgca cggcggattt cgtcgtcacg accgataacg gggtcgagct
    15781 taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt tttcagcgca tcgcgttggt
    15841 cttcggcatt ggcatcgttc acgttttgtc ctcctcgtac cgcgtcaatc gcggcattga
    15901 tgttttgttc ggtcgcgccg gcttctttca aaattttgcc ggtcgcatcg ttctgctgca
    15961 ccaaggcaag caggaaaagt tcactggcga tataggcatc accgcgtttg gtggcagcct
    16021 tgtccatcag gttcaacacc gcctgcaatt cgcggctggg cagaatgtcg ccgccctgac
    16081 cggacacttt cggcaggctg tttaaatgct gctgcaaacg ctgtttcact tgcggcatgt
    16141 tcacgcccgc atgagccaag agcgcggcgg ctccgctgtt ttggtcgtca agcagggctt
    16201 ttaacacaaa gcccgcttcc agatagctgc cgtccgcagc caacgccaaa ctctgagctt
    16261 ctgcaagggc ttgttggaat ttggcggtta atttgtcgta tcgcattttt gtttcctttt
    16321 caaaatgtcc gctgtcgaag cctatatgtg cataattgtg gataactcaa gttctgtttt
    16381 ctgtttttct atatttaatt cgatatatca ttgaatttaa agtatataaa aatgtataat
    16441 aatgtgtata actatatctt cttaatatgg aaaagtctgt tgtcggctgg atgtaggtgg
    16501 caaatcgggt ataatcggcg catctttttc cctttcagac ggcattgatg ccgcaaggac
    16561 atttttatga gcaaaaaacg agttctgacc ggcgtaacca ccaccggcat cccgcatttg
    16621 ggcaactacg tcggcgccat ccgccccgcc gtccgcgcgg cgcaaaaccc cgataccgaa
    16681 tccttcctct tcctcgccga ctaccacggc atcatcaaat gccacgagcc ggagatgatt
    16741 caccaatcca cccaagccgt tgccgccact tggcttgcct gcggactcga ccccgagcgc
    16801 accaccttct accgccaaag cgacattccc gaagtgatgg aattgaactg gattctgacc
    16861 tgcattaccg ccaagggttt gatgaatcgc gcccacgcct acaaagccgc cgtgcaggca
    16921 aatgcggaaa acgggcagga agaccccgat tttggcgtgg aaatgggttt gttcagttat
    16981 ccgattctga tgactgccga catccttatg ttcaacgcca acgaagtgcc cgtcggacgc
    17041 gaccaaatcc aacacgtcga aatggcgcgc gacattgccg gacgcttcaa ccaccgtttc
    17101 caagaactct tcaccctgcc cgaagtgaaa atcgacgaaa acgtcgaact cttggtcggt
    17161 ttggacggac gcaaaatgtc caaatcctac ggcaacacca ttccgctttg ggaaaacgac
    17221 aaaaaaaccc aaaaatcggt caacaaaatc atcaccaata tgaaagagcc gggcgagccg
    17281 aaacagcccg acgaaagccc attgtttgaa atctacaaag ccttctccac gccgtctgaa
    17341 acggcagaat ttacgaaaat gcttgccgat ggcttggctt ggggcgaggc aaaaaaactt
    17401 ttggcggcga aaatcaacgc cgaactcgcc gaaccgcgcg aacgctacaa cgagctgacc
    17461 gccgaccctt cgcaaatcga agagattttg caggcaggcg cgcaaaaagc gcgtaaagaa
    17521 gcacgcgaat tgttggacaa agtacgcgat gcggtcggca tccgcccgtt gaaataaacc
    17581 cgatgccgtc cgaacctctt aaaggcttgc cggaacaaaa cgtcgtccgg ctgaccggca
    17641 agcatcccgg cgacttggaa gccgtcagcg gcaaatgtat ggaaaccgac ggaaagggcg
    17701 caccttcggg ctgggcggca aacggcgtgt gccatacctt gtttgccaaa ctggtgggca
    17761 atatcgccga agacggcggc aaactgacgg attacctgat ttcgcattcc gccctgcaac
    17821 cctatcaggc aggcaaaagc ggctatgccg ccgtgcagaa cggacgctat gtgctggaaa
    17881 tcgacagcga gggggcgttt tatttccgcc gccgccatta ttgaggtatt cggacatccc
    17941 ggaatatatt tgggtttttc aaaccctgca ggaaaaagtc cgcaccgtcg ggaaactcaa
    18001 aaggaggggg atatgtgtta caattttccg aactgttttc ataaaatagt tttcggacgt
    18061 gtttcaatat ggcattgatg ccgccttatt gttcgagaaa aaaaccttta tatttaaata
    18121 taattgggtt ttaactaaat gggaaaccgt tttctctccg gccgatgggc aaaatcagcc
    18181 gattgatgga acacggtccg atttttaagc aaaaccttaa tcaacaactc aagaaggata
    18241 agacatgaaa aaattattga ttgccgcaat gatggcggct gccttggcag cttgttcgca
    18301 agaagccaaa caggaggtta aggaagcggt tcaagccgtt gagtccgatg ttaaagacac
    18361 tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa gaagcgaaag accaagtcaa
    18421 agatgctgcg gctgatgcaa aggcaagtgc cgaggaagct gtaactgaag ccaaagaagc
    18481 tgtaactgaa gcagctaaag atactttgaa caaagctgcc gacgcgactc aggaagcggc
    18541 agacaaaatg aaagatgccg ccaaataatt tgttgccttg gcaaaaatga tgggatgccg
    18601 cctgccggca acccaaacga aaccgcctga agattttcag gcggtttttg agttgtatgc
    18661 cccttgtttt ttacgcctgc atgaccgttc cgagcagatg tgccgcgcat atcgggatgt
    18721 tgcggacagg caaatgccgt ctggacaggg tttggacgga atttgttccg taaggttcag
    18781 acggcctcga ctttaaacgg catattgatt ttttgccatt ggacgctttc atattccaat
    18841 gcgtcggcaa tcaggggatg gcgttccagc cattccctgt caatacgcag gatgaagccg
    18901 cagctttccg tatccgtgcg caactgcata tttttcggga aagacaggtc ttggcgcgaa
    18961 cggcagaaca gggcagcaag gcgcagggac aaaacggcat accacaacat ttcgttggtg
    19021 ccgatgatgc cgctcatttt tttcatatcg ccgcgatgac cgatgaccag ttgggcaagt
    19081 atggtttgtt ccttgcgtga aaagcccggc atatcggcgt tttcgaggat gtaggcggaa
    19141 tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt cgtgcagcgc ggcggcgcgt
    19201 ccgagatact gttgccacaa ggcaagctct tgaaccgtaa catttttagc gtggcagagg
    19261 ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg cctgattgag gctgacgtgg
    19321 tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat cttcgtttaa accgcgcccg
    19381 atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg ttacggtcat cctgtcgagt
    19441 ttcatttctt caaacgccgc catcatcacg gcaagtccgc cggcaaaaac ttcgatgcgt
    19501 tccggtttca ggttttcaaa tttggctttt ttgaccgaac cggcttcgat gatgcgttcg
    19561 gcgagggcgc gcattccttt gtaggtaatg tccgcctctt ggggcatttc ggcggcaagc
    19621 acgtcgcgga tggattttgc cgaacccgat gtgccgacgg cggaatccca accttcgcgc
    19681 ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg cggaaatggc ggcttggaag
    19741 tctttggcga tgattttgtt ttggaagaag cgcaggctgt aggttacgca gcccaagggc
    19801 aggctttcag taatgtcggg attcagcgtc gagccgatga caaattctgt cgaaccgccg
    19861 ccgatgtcga taaccagcat tttgccgccg cccgggggga gggtgtggat cacgccggta
    19921 taaatcagcc gcgcctcttc gcgcccggcg atgatttcga tggggaaacc cagtgccgct
    19981 tcggctttgg gaaggaaatc tgcgatgttt ttggcgacgc ggaatgtatt ggttgccacg
    20041 gcgcgtacct gttcagggcg gaagccgcgc aggcgttcgc cgaattttgc cagacagtcc
    20101 aaagcctgtt cttgggaagc ggcactcagg tttttctgtt cgtccagtcc ggcggcgaag
    20161 cgcaccatct gtttgaacga atcgatgact tttaattgtc cgttgttgtt ttcgcaaatc
    20221 tggaggcgga aactgttgga acccaaatcg acggaggcga ggacgtttgc gggatgggtg
    20281 gtcatggtgg ataccggtgg gggaaaaacg caatgttact ctgacggcgc aggcgttgac
    20341 aataaatgat gcggcggttt ttgattctgc ccacggatgt tgccgacggc atttttgcgc
    20401 ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg tttttgcgtg tgtaaaaact
    20461 tttcatattg ctgtcgattt tcagatagcg gaaattgagt tgcggcgtaa agcccttcca
    20521 agagattttg tcgtgccaca acgacaggtt tgcctgatat tcgtggtctt tgcgcgggaa
    20581 gcggtacaca atggtcccgg gtgcgtcaaa catcctgcgg gtatagcgca ggtttgcccg
    20641 cagacccaag cccgccgtcg aacgttttga ctgcgccgac acgcaaaccc ttgcggacgg
    20701 aagcctgttc cgcctctttc gtcatgttgt gcgaccagtc cgcaccgccg taaagcagcc
    20761 agtctttagg cgcggaatac atcagcgtcg cgcccgccag cggcatatgg ctgtcgtatc
    20821 gggcggcggt gcggtcttcc tgataacgct tccacatatt gcccacgttc agcgtcagcc
    20881 gccagcgttc gctcaagcgt cgggagaaat cggcattgaa gccgccgacg aaattgtatc
    20941 ggctgccgcc taagaggttt tgcccgataa acggcacgat gccgaacgag cgcgttaccg
    21001 aacgattttt atagccgagc gacaggcgca ggcactgttc gctgaaatct ttgttatccc
    21061 aataatgcac gccgccgccg ctgatgccgc cgtagaggaa atgatgcccg cccgcattga
    21121 tttcgcgcga cacgcctaag ccctagcgca aaccgtgtgc cttttgcggc aggctgtcgg
    21181 cggttttcgt ccagcttctg cccgcaaatt caatcgtttt tttggacgaa gcgttgttta
    21241 tag
//
LOCUS       DAXIEB010000023        20990 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000023 DAXIEB010000000
VERSION     DAXIEB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.023"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..181
                     /locus_tag="ACFOA3_000732"
     CDS             <1..181
                     /locus_tag="ACFOA3_000732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897770.1"
                     /translation="QVLDVVSFALGQLRVAHGNLSCRKGRMLPHTGLFLLGKVALQMR
                     IRRSLKFDAPYILRP"
     gene            complement(301..2001)
                     /gene="recJ"
                     /locus_tag="ACFOA3_000733"
     CDS             complement(301..2001)
                     /gene="recJ"
                     /locus_tag="ACFOA3_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6897771.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(2070..2786)
                     /locus_tag="ACFOA3_000734"
     CDS             complement(2070..2786)
                     /locus_tag="ACFOA3_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897772.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFKAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENILHTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(2833..3339)
                     /gene="yjgA"
                     /locus_tag="ACFOA3_000735"
     CDS             complement(2833..3339)
                     /gene="yjgA"
                     /locus_tag="ACFOA3_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6897773.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTKLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            3493..4824
                     /gene="pmbA"
                     /locus_tag="ACFOA3_000736"
     CDS             3493..4824
                     /gene="pmbA"
                     /locus_tag="ACFOA3_000736"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6897774.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFATHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(5121..5324)
                     /locus_tag="ACFOA3_000737"
     CDS             complement(5121..5324)
                     /locus_tag="ACFOA3_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6897775.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            5567..5869
                     /gene="clpS"
                     /locus_tag="ACFOA3_000738"
     CDS             5567..5869
                     /gene="clpS"
                     /locus_tag="ACFOA3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6897776.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5871..8168
                     /gene="clpA"
                     /locus_tag="ACFOA3_000739"
     CDS             5871..8168
                     /gene="clpA"
                     /locus_tag="ACFOA3_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6897777.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLD
                     KKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRI
                     DWDAAKEEAVLKFKKNKVKPEAEPV"
     gene            complement(8216..11314)
                     /locus_tag="ACFOA3_000740"
                     /pseudo
     CDS             complement(8216..11314)
                     /locus_tag="ACFOA3_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(11345..11454)
                     /locus_tag="ACFOA3_000741"
                     /pseudo
     CDS             complement(11345..11454)
                     /locus_tag="ACFOA3_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11499..12095)
                     /locus_tag="ACFOA3_000742"
     CDS             complement(11499..12095)
                     /locus_tag="ACFOA3_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6897778.1"
                     /translation="MVWKTLGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGR
                     ILDNSLKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEF
                     KDALDINFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILD
                     KFDTLTHSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(12125..12718)
                     /locus_tag="ACFOA3_000743"
     CDS             complement(12125..12718)
                     /locus_tag="ACFOA3_000743"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6897779.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGG"
     gene            complement(12884..13894)
                     /gene="rhuM"
                     /locus_tag="ACFOA3_000744"
     CDS             complement(12884..13894)
                     /gene="rhuM"
                     /locus_tag="ACFOA3_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFC6897780.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTVAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(13891..15435)
                     /locus_tag="ACFOA3_000745"
     CDS             complement(13891..15435)
                     /locus_tag="ACFOA3_000745"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6897781.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIVVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(15508..16512)
                     /gene="rfaD"
                     /locus_tag="ACFOA3_000746"
     CDS             complement(15508..16512)
                     /gene="rfaD"
                     /locus_tag="ACFOA3_000746"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6897782.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(16553..17672)
                     /locus_tag="ACFOA3_000747"
                     /pseudo
     CDS             complement(16553..17672)
                     /locus_tag="ACFOA3_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(17674..18908)
                     /locus_tag="ACFOA3_000748"
                     /pseudo
     CDS             complement(17674..18908)
                     /locus_tag="ACFOA3_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(18945..19916)
                     /gene="hldA"
                     /locus_tag="ACFOA3_000749"
     CDS             complement(18945..19916)
                     /gene="hldA"
                     /locus_tag="ACFOA3_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6897783.1"
                     /translation="MPAKFQQETLNSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHEGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAILLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGVG
                     LGLAAGYTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQSTM"
     gene            complement(19972..20712)
                     /gene="pyrF"
                     /locus_tag="ACFOA3_000750"
     CDS             complement(19972..20712)
                     /gene="pyrF"
                     /locus_tag="ACFOA3_000750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6897784.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRSLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPLAVLREVNRVANLEAN"
BASE COUNT         4899 a         5323 c         5138 g         5630 t
ORIGIN      
        1 acaggtattg gatgtggtat cgttcgcctt gggtcagttg cgtgtagctc atggcaatct
       61 ttcttgcagg aaaggtcgta tgctaccgca tactggcctt tttctgttag ggaaagttgc
      121 acttcaaatg cgaatccgcc ggagtctgaa atttgatgca ccttatatat tgcgtcccta
      181 agaagggatg attaacaaaa attaacgccc tttactttct tttccgattg ggaacattcc
      241 cgtcgtacaa acaggcaaat gaaggaacag aaatcacatt caaacggcgt tccgccgcct
      301 ctacaccgct tcccagtaat cgatatacag ctgcaattcg agattgttgc gccattcgtt
      361 ggcaacgggg cggtaaaccg tgcggatgta ttcggggatg tcttcgctgc aacgccaaaa
      421 catcgcttca aattcgcagc cgtctttttg cagccagact tttttgtgtt tgccctccgc
      481 gcccaaaggt tgctggcgga cgacgtggaa ctcgtcggta aagctcggcg gcgcgaagcc
      541 ctgcccccaa acgtgacggg caaggttttg cgcctgttcc aacgtgatgt cgcaggcggg
      601 caggctgccg tcggtgatga aggtttgcga caaatcgtct tcgcacacca tttcgcgcac
      661 ggcttcttca aaggtcgtct gaaacgcggg aatgttgtgt tcaagtatgc tcaaacccgc
      721 cgccatcgcg tgtccgccga attttaaaat caaatcggga tggcgtttgg acaccaagtc
      781 caaagcatcg cgcaagtgaa gattgggaat ggaacgcccc gaaccgcgca cttcaccgtt
      841 gtcggcaggc gcaaacacga tggtcggacg ataaaaacgg tctttgaggc ggctggcaac
      901 gatgccgacc acgccttgat ggaagtcgtc gcgatacgcc accaaagtca tctgacctga
      961 aggcagggtt tcaggaaaag cattcagcgc gtcttgcagc atagactgct cgatttcgcg
     1021 acgttcgata ttgaggttgt ttaactgcgc cgccagttcc tgcgcctcgg tatcatctcg
     1081 cgccaacagg caggcaatac cgaccgacat atcgtccagc cgtccggcgg cgttgatgcg
     1141 cgggcccaac gcaaaaccca tatcaaacgg ctgcgccttg cgccaatcgc gccgcgccac
     1201 ttcaaacaag gcgcggatac cggggcgcat tttgccggag cgcatccgtt tcaaaccttg
     1261 cgacacgagg atgcggttgt tgtggtcgag agggacgaca tcggcaaccg tgccgagtgc
     1321 gaccaaatcc aaaagttcgc ccagattcgg ctctttgatg ccgtctgaaa aataattgcg
     1381 gcggcgcaat tcggcacgca aagccatcaa tacataaaaa atcacgccca cgcccgccaa
     1441 gcttttgctt ggaaaaccgc agcctttttg gttcggattg acgatgatgc agtcgggcac
     1501 ggtctcggca ggcagatggt ggtcggtaac gatgacatcc aaacccagag cctgcgcgcg
     1561 cgccacgcct gcgatgctgg cgataccgtt atcaaccgta atcagcaaat ccacaccttg
     1621 ctctgccgcg atttcagcca gctcgggcgt taagccgtag ccgtgctcaa agcggttggg
     1681 cacaaggaaa tccactttcg cgcccatcgc cgccaaaccg tccaaaccga cggcacacgc
     1741 cgtcgcgccg tcggcatcat agtcggcgac aatcaggatt ttttcctgac gctcaaccgc
     1801 atccgccaaa cggccggcgg cggcttcgca attcgtcagc gattgataag gcaggaggga
     1861 agcgagtttg tcgtccaatt cggcaggact ttgcacaccg cgcgaagcac aaagccgggc
     1921 gattaaagga tcggcaccgg cggtgagcaa atgattaaaa acgtcggtat tgatggatcg
     1981 ggtttggatt ttgactgaca taacgtgggg ttttactgtg cgtgtgtgct taaaatgccg
     2041 tctgaaagct tttcagacgg cattgctgtt catttgcggg cggcatccac gaccgcaccc
     2101 agagccgccg caggcaaggc cagtaccgcg cccgccgcat ccagtatcaa caaggggggc
     2161 gtatgtaaga tattttcaaa cagcttggat ttgtcggtat gttttttcgt aaccgtgtaa
     2221 taaatatcgg caggcacact ttgctcaaaa tgataatcgg cgttcagttt ttgcggtgtg
     2281 gcgtagtatt tgcctttggc ggagacgcag cgcgtgtaaa tggtccgatt gtcgagttcg
     2341 accgctttaa actcaagctg tttcagcttg gcgatgtcag caggcctgtc ggtatcgtag
     2401 cgcaggcaaa ggccttcggt actgaaattc tggctgccag gcgattcgag tttgaccggc
     2461 agggcttggt ggcgagcata gctcggggta tcctcaacta tttggaaggg tttgtccagc
     2521 cctgccttca aaatgcccgt cagcttcgcc gaatcttcgg gattgacgac gaaccagtat
     2581 ttcccgccca tcatcaccag actgcccttt tccaattggg cattgtcttt ggcaaccaca
     2641 ccgaaggcgc ggatttgatc cttgtcaacg tgtttatagg cggtcgtttg gctgaacggg
     2701 tcgttcatgc cccacatcat tgccgtacag ccattcagca tcaaggttgc cgccaaaacg
     2761 gcggcggtcg ttttacggaa caacatttta tcctccaatc taaataacgg cggatgtcgt
     2821 ctgaaaatat gcctaaattt ccgcgtcccc gttttccatc acggttttca attcttgaaa
     2881 cagcgcgcgg aagtttttcg gcggtttgtt ttgctcctgc tcttttttgg tgttgcggac
     2941 gagcgtcctc agcttgcccg cgtccgcatg cggaaaatcc gacataaact gcgtcaacgc
     3001 gccgtcgtct gccaacagcc gtacgcgcgc ctgttccaca cgttgcaaaa aggcattgtg
     3061 caccgcatcg tcgccgcgca gcttggcaag aaacgcctcg atgggcgcgg gatccgtgtc
     3121 gcgcatcagc ctgccgataa attgcgcctg ccgcttgagc gcgccgttgg acgtaatttt
     3181 tttataggta acgacggctt cgtacaaatc ttcgtccaag ccgatttttt tcagcgtgtc
     3241 gtttgagagc ttggtcagtt ccatacccaa atcctgcaaa ccgttcatct gctttttcat
     3301 ttgggttttg ctgatccatt cgtcttcttg ttcaaacatc ttgattctcg aattttttcc
     3361 aatagcgcat tttaacagaa tgccgccgcg ttcagacggc attttgcacc gcacgccctg
     3421 caagcggcgc gcttttgccc ctgccgcccg aaacggttaa aatgccgcaa agcatcatca
     3481 agaaagattt ctatgctgtt caaccacacc gcttccgaac tcctcgacct ctgccgccgc
     3541 acgctcgact tggcaaaagc gacgggcgca accgccgccg aagccgattt cagcgaatca
     3601 ttgggacaaa gcgtcagcgt acggctgggc gaaatcgaac aaatcgagtt ccagcaggac
     3661 aagtcgctgg acattaccgt ttacgtcggc aaacgcaaag gccgcgccag cactgccgac
     3721 ttctccgaaa aagccctgca agacaccgtc aaagccgcca tcgacatcgc ccgctacact
     3781 gccgaagacg attgcgcagg tttggcagac gcttcgctta tggcgcaaca catcggcgac
     3841 cccgaccttt accacgaatg ggatttggat acggaagccg ccgtcggctt ggcaaaacaa
     3901 tgcgaacaag ccgccctgaa cgaggatgag cgcatcgaaa actccgaagg cgcggcggtg
     3961 caaaccggcc attaccaata cgtttacggc aacacccacg gttttgccac acaccggcaa
     4021 ggcacgcacc acagcatttc ctgcagcgtc gttgccgccg acgaaaacgg gatgcagcgc
     4081 gactactggt acgattccgc ctgccgccat ccggatatgg acagccccga aaccatcggt
     4141 caaaccgccg cccgccgcac tttgcgccga ctcggcagcc gcagcatccc gacaggcagc
     4201 taccccgtcc tcttcgatac caccgtttcg ggcggtctta tcggacacct cgtcggcgca
     4261 ctctccggcg gcgcgctcta ccgccaaagc agtttcctga tcgacagcat cggcaaaaaa
     4321 gtcctgcccg atttcctcaa cctgcgcgaa gaaccgcaca tcccccgctc tttccgcagc
     4381 agctattttg atgcggaagg cgttgccacc gcaccgcgct tcgtgattca aaacggcatt
     4441 gtcaaaggct atttcctcag cagttacagc gcgcgcaaac tcggtatgca gaccacgggc
     4501 aacgccggcg gcgcgcacaa cctgtatttg aaccatacgc acgaaacgca atccgacctg
     4561 ctgaaagaaa tggggacggg attattggtt accgaactga tgggacaggg cgcgaacacc
     4621 attaccggtg actactcgcg cggcgcggcg ggtttttggg tggaaaacgg cgtgattgcc
     4681 taccccgtcc acgagattac cgtagccgga cgcttgcagg atatgtaccg cgacattgtc
     4741 ggcgtggcgg atgacgcttt gcggcgttcg tccaacaaaa tcggctcgat tctgattgcg
     4801 gggatgacgg ttgccggaag ctgaacgccc tgccgatgcc gtctgaagac gaaggcagca
     4861 cgttttgccc cgtgcctacc cgtttgccgt tttatgccgc accgcctttt gccgacaaca
     4921 ttgcacattt tttcatcagc actgattttc ccttatccgt caaaatacta agccacttgt
     4981 ccacaacagg gtacacacaa gttcaggata aggcgtgcgg ggattggggt ttttccgccg
     5041 tcggcaaaac ggcagacaaa aacggcggga cattgcctgt cccgccgttt tcaaatatac
     5101 atttgaccgt acatcaggaa ttaagcagcc tgaatgttgg cggcctgttt gcctttaggg
     5161 ccggtggtta cgtcgaaaga gacgcgttgg ccttctttca gggttttgaa accttccata
     5221 ttgattgctg agaagtgagc gaacaaatct tcgccgcctt catcaggcgt gatgaaacca
     5281 aaacctttag cgtcgttaaa ccattttacg ataccggttg ccattagaaa cttcctatac
     5341 tcaaaaatta acaaaatcag caaaacaagg catacggcaa accgagccgt ctggcgttcc
     5401 ttccagcttg atatactcgg cggatgccca atcctgctta accgtctttt tacatttgtt
     5461 aagacaaaac gtcaagctat gttttcaaaa aagtgggaaa ataggcaaaa gtaccgaaaa
     5521 tacaacaaaa atgccgtctg aacgcggctt tcaaatggga cacactatga ccgcgcaaca
     5581 ccaatccgac acgctcttgc accgtctgaa caccctgccg ccgaaacgtt acggcgtttt
     5641 cctattgaac gacgattaca ccacgatgga atttgtcgtt gaaatcctga ccgaaatctt
     5701 tatgctcgga caagaacagg cggtagcggt aatgctcttg gttcatcacg aaggcaaagg
     5761 cctgtgcggc acttacacgc gcgatattgc ccaaaccaaa cagcaacaag tcatgcagcg
     5821 ggcaaaagcc gaagggcatc cgctgcaatg tattgtcgag gagatttaat atgcttgcac
     5881 ccgaattgga acagattttg cagcagcttt accgcgaggc gcgtaaggct cattatgaat
     5941 ttatcagcct cgagcatctg cttttggtac tcatcgaaga agatgccgcc gtgccgaacg
     6001 tcttaaaact ctgcggcgcg gatttgaaag tggtgtccga acagctcgcc gccagcgttg
     6061 ccgaaaacac ccccctgatt cccgaacacc ttttagacac ggtcgaaacc cggcccacgc
     6121 tcggcttcca acgcgtgatg caacgggcga tggtgcatac ccagtctgcc ggaaaagccg
     6181 cagtcgaacc gttggacgtt ttggtcgcgc tgatgagcga aaccgacagc cacaccgtct
     6241 atttcctcaa gctgcaatcg gttacgcgtt ttgaagtttt gcgctgtatt gcccacggct
     6301 ctcccgatga agatgaagat gaagacgatg gcaactattc ttcagacggc atggacgacg
     6361 ataatgaaaa ccgcaccaaa ccgggcaaaa accctttatc ggcgtacacc gtcaacctca
     6421 acgccgaagt caaagccggc cgtatcgacc ctttgattgg tcgcaaacac gaaatggaac
     6481 ggctggtgca aatcctatgc cgccgccgca aaaacaatcc gcttttggtc ggcgaagcgg
     6541 gcgtgggcaa aaccgcgctg gcggaaggtt tggcacatca aatcgtcaac ggcggcattc
     6601 cagacgcgct taaagatgcc gaagtgtacg cgctggatat gggctcgctg ttggcgggca
     6661 cgaaataccg cggcgacttt gaagcgcggg tcaaatccgt cttgaaacag ctcgaaaaaa
     6721 tcccgcacgc cattttgttt atcgacgaaa tccacaccat catcggcgcg ggcagcacca
     6781 gcggcggcac catggacgcg tccaacctgc tcaaacccgc gctggcaaaa ggttcgctgc
     6841 gctgcatcgg cgcgaccacc tacgacgaat accgcaccat tttcgacaaa gaccatgcct
     6901 taagccgccg cttccaaaaa atcgacgtgg tcgaacccac cgtttccgaa accgttcaaa
     6961 tcctgcgcgg cttgaaaccg atgtttgaag ccttccacca agtccgctac actcaaggcg
     7021 cactcgaagc cgccgccgaa ctctccgcac gctacatcaa cgagcgtttc ctgcccgaca
     7081 aagccatcga cgtaatggat gaagcaggcg cggcgcaacg gattctgccc aaatccaaac
     7141 agaaaaaagt catcggcaaa gcacaaatcg aaaccgtcat cgccaaagtt tcgcggattc
     7201 ccgaaaaaac cgtgtcgcac gacgacaaac aagtgctgca attcctcggt cgcgatttga
     7261 aaaacatggt ttacggtcaa gaaaacgcca tcgacgcgct ggttgccgcc gtcaaaatgt
     7321 cgcgttccgg ccttgccctg cccgacaaac cgataggcag cttcctcttc tccggcccga
     7381 ccggcgtggg caaaaccgaa gccgccaaac agcttgccta ctcgctgggc gtaccgctgc
     7441 aacgctttga tatgtccgaa tacatggaac gccatgccgt atcgcgcctc atcggcgcgc
     7501 caccgggcta cgtcggcttt gaacaaggcg gccttttgac cgaagccgtc aacaaacagc
     7561 cgcattgcgt attgctcttg gacgaaatcg aaaaagccca ccccgacatt ttcaacgtcc
     7621 tcctgcaagt catggacgca ggcaaactga ccgacaacaa cggcaagagt gccgatttcc
     7681 gcaacgtcat cctgattatg accaccaacg caggcgcgga aagcctcagc cgccccagcc
     7741 tcggctttac cgccaagcgc gaacgcggcg acgaaatgca ggcaatcaac aagctcttca
     7801 cgcccgagtt ccgcaaccgc ttggacgcga ttatcccgtt tgcacccttg tccgaaccca
     7861 tcatcgtcaa agtcgtggac aaattcctgc tccagctcga acaccagctc ctcgacaaaa
     7921 aagtcgaagc cgaattcacg tcggcattgc acaaatatct ggcggaaaaa ggtttcgacc
     7981 cgcaaatggg tgcgcgcccg atgcaccgcc tgattcagga aaaaatccgc aaaccgctcg
     8041 ccgacgaact gctgttcggc aaactcgccg acggcggctt tgtgcggata gactgggatg
     8101 ctgcaaaaga agaagccgtg ttgaaattca agaaaaacaa agtcaaacct gaagcagaac
     8161 ctgtttaacc agttgaaaaa taaaaagccg tctgaaaggt tcagacggca ttttgtcaaa
     8221 tatcggtccc tattccggca aacttctcca caaacgccgc aatcttttga aaaacacttt
     8281 gtttcttcgt cagatattgc gggttgagcg gactcatttt cggcagggtt tcggtcagtt
     8341 ccgtaccgtt ttcgctggca tagccgcgtt tgagcgagcc ggtcaaatag cgtttggcgg
     8401 cggtttcgtt cagaccttcg gcggcaatca atcctgccgc ttcgtgccgc atcacctctt
     8461 gcgcgtaggt gtagaaggtt tccagtatgg cgggaacgtc ggggactttg tccaaatccg
     8521 tatcgttgat gaaatccaca atcagaccct ctttggcgcg gtgtccgata cttgcgcgga
     8581 tgatgcggcg gatttcttcc accagctccg ctttgccttt gatttttttg tggtgctcga
     8641 aaaccagttg caggatgtaa tccaaattga tttcctgcga tttgagcaaa tccacctcaa
     8701 aaaccacatc gtcccagtcg atttttgact gttctttctg ttcgcctgct ttttggcggc
     8761 gcaaccaatc gcggatgtcg ttgtaggcgg aacggtagtc ctgcaccgcc ctttcagacg
     8821 gcatcggcac ttgccgcatt tcctgcacgt cttcatcgct caggtagtat ttttcttgaa
     8881 acgccttcat cgcatcttcg tccgccgcgt ccacactctg caactcgcgc agcgcggcaa
     8941 attcatcgta gttctgcaat acgttttccg cccgcaggta ttcgccgaag agtttggcaa
     9001 aatctttttt gtctttttcc gtttcgattt tgtcgggttc ggggaaacgc tcgcgcaatt
     9061 cttttgccac atccaaatag ccgcgccgtg cttcgcccgt ccgatcatcg atatagccgt
     9121 tcatgtattc ttcgtaactt ttttccagca ctacattttt ggtgtttttg tcgccaaaca
     9181 aggtaatcgc atcaatggtt gcctgctcca aatcgcggaa gcagacaata ttgccgaagg
     9241 ttttggtggc atcgtaaatg cggttggtgc gcgaaaacgc ctgcatcagg ccgtgatagc
     9301 gcaggttttt atcgacgaac aacgtgttca gcgtcggcgc gtcaaaaccc gtcaaaaaca
     9361 tgccgaccac aatcagcaaa tctatttcct gatttttcac ccgttttgcc aaatctcggt
     9421 agtagttttg aaaggctttg ctgtccgtgc cgaaattggt tttgaaacag gcgttgtaat
     9481 cgttgatggc agcctgcaaa aattcttttg cgctgctgtc catcgcttcc ggttcaaaag
     9541 tctcatcgac aatttcaccg acggcgtttt gctcttcgtt ggccgcaaag gaaaaaatgg
     9601 tggccacttt cagcgggtgc aagctgcctg cctgttgtgt tttgaacgct tcgtaatagc
     9661 acttcgccgc atccacgctg ctgacggcaa acatcgcgtt aaagcctttg ccacccgcat
     9721 tcagccggtg cgttttctgc ctgaactggt tcaggatata ttgcgtgatt tcgcggatgc
     9781 gttcggggtg cagcagggct tggcggtttt cggcggcact cagtttcttc tcgtcctgtt
     9841 ccgcttccac ggctttgaac tgcgggcgca catcgttgta atccactttg aatttcaata
     9901 ctttttcatc gcggatggca tcggtaatca cataagaatg caactcccgc ccgaacacgc
     9961 ccgccgtggt ttccgcgccc aaagcgtttt cgggaaaaat cggcgtgccg gtaaagccga
    10021 actggcagaa ttttttaaat ttctttttca ggtttttttg cgcttcgccg aattgcgagc
    10081 ggtggcattc gtcgaaaata aagacaaccc gctgatggta aatcggcaga ttatcttcac
    10141 tcttcatcag gttattcagc ttttggatgg tggtaacgat aattttgttg tcgtcttttt
    10201 ccaaattgcg tttcaagccc gccgtgcttt ccgaaccgtt cacgctgtcg gtcgaaaaac
    10261 gttggtattc cttcattgtc tgatagtcca aatccttcct gtccaccacg aagaaaacct
    10321 tgtcgataaa cgccgattcc gtcgccagat gcgccgcctt aaagctggtc agcgttttgc
    10381 cgctgcccgt ggtgtgccag acatagccgc cactctccgg cttgctccaa ttcttcgcct
    10441 gcgccgagct gttgattttc cacaaaatgc gttcggcggc ggcaatctga tacggccgca
    10501 taatcagcag cgtattattc gcatcgaaca cgctgtaatg cagcaaaacg ctcagcaata
    10561 cgcttttctg caggaacgtg tcggtaaagt ctttcaaatc cttaatcgga tgattgtccg
    10621 accgcgccca attcatcgtg aaatcgaagc tgtttttgtc gcgcttggtg gtgttggcga
    10681 aatagcgcgt gtccgtgccg ttggaaatga cgaagatttg caggaatttg aacaacgaat
    10741 tgtcgctgtt gaagctctct ttgctgtaac ggtgcacctg attgaatgct tcacgcaccg
    10801 ccacaccgcg ctttttcaat tcaatctgca ccagcggcag gccgtttacc aacacggtaa
    10861 tgtcataacg gtttgcatgc gtgcccgtct gctcaaacta gttgataagc tgcacatggt
    10921 tgcgggcaag gtttttcttg tccagcagat aaatgttttt cagacggccg tcatcaaaag
    10981 cgaaatcgta aatatggtcg tcgtggattt tgcgggtttt gtcggtaatg ttttcagacg
    11041 gcctgtccag atattccgtt aaaaaccgcg cccattcgcc gtctgaaaac gccacatcgt
    11101 tcagccgctg caactgcgcg cgcaggtttt ccagcagcct gctttggctg ttcaagtctt
    11161 tgcggtattc gtaaccctga ttctgcaaat cggcgattaa ctccgcttcc aactggtttt
    11221 ccgattggta gctgcccgac tgttcgattt tttcatattg gtcgagcacg atgaaattcg
    11281 gcgtttcagc gatgggttta gtttcgaggt tcatatgggt cgtcctttgc ggtgtcgggt
    11341 tttatagtga attaaattta aaccggtact gcgttggctc gccttgccgt actatttgta
    11401 ctgtctgcgg ctcgccgcct tgtcctgatt taaatttaat tcactataaa cggtttgatg
    11461 ttcggattgg gctgcctgaa aatgtgcagg ctgctttttc aggtagcctt agggaaggca
    11521 agcagctgtt cgcggtaata ttcgtattgt ttgcggcgca gggcgatttc gtgcggcagg
    11581 ccttcgctga tagagtgggt cagggtatcg aatttgtcta ggatggcggc aattttttcc
    11641 tgttcgggga gtggtgggat ggggattttt aatttcttta attctttcat gctaataata
    11701 ggtaaacttg aagtattaat aagttttttg ctctgctccg caataatgaa aaaataatag
    11761 aaagcgaaat ttatatctaa agcatcctta aattcatctt taattgtaaa attggtcagt
    11821 tgttggttac taatataatt aacttttatt aacgcatgct ctcctatggt tgccgttgtc
    11881 gacatcatta ttgatttagc aggaaacagc ttgccgccct taactgcgct ttttgatata
    11941 tgcttcaatg aattatctaa aatacggcca ttttccctaa tatcttccat tctaaaccaa
    12001 gggattgagc cattttccca atattcttta tttgatttgg aaggggtata gccattcttc
    12061 aagtcaaaca cctcccccaa cgtcttccaa accacatctt tcagacggcc tttatagcca
    12121 tcagctatcc ccccccgatt tgattgtcaa aatctaaaag aaagtcgcgg taataccggt
    12181 attggcgttt gcgcagggct aattcagctt ccagctcggt gaatttgtca agtattttta
    12241 caattttttg ttgggtttcc aggggtggga tagggattaa taattttttc aagttactgg
    12301 catttagagc aggaatacct gtaaatgtac ataaggcatg aatctcttgt tcaaattcaa
    12361 gtaatatatg atagagaaaa cgaacatcca attctgttct attcttaata gtaactgcat
    12421 aatttaagtt gccacgaaaa tatcttcctt cttgccacgt tattgagccg actcccgctc
    12481 ctcttgttgt gatgccgata gggtcatttt cagtgttcca ttcatcaata tagccaagcg
    12541 gctctttgcc actgttaata actggataac taccaatatt atttgagatt ttctgcttac
    12601 ttacagcttg tcctgttttt atgatagcta tcccattttc tccacccaac ggcttccact
    12661 ccaccggtgc cgtctgaatc atctcaatca attttttcgc tttgctttgc atatccatgg
    12721 ttgttttccg tttctttccc gctgtggcgg gatgtggggc ggttgtgggg tgcttcggct
    12781 cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc gtcctgcttt caggctgcct
    12841 gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg tagttatttt gtgcggtctt
    12901 ttttctgttt gatctgttgc acggctgttt tttccaacgc tttaacgctt tccagatagg
    12961 cttgctccac cggcgacagg atgcgcgctt cataggcgcg gtattcgcgt tcggctttct
    13021 gctctgcctg tttgcggctg atgctgcctg caccctgtaa tgtgccttgt ccgtacagcc
    13081 cggaaaattt gtccaattcg gctatccagt cgcgcatata catggggctt tgctcctgcg
    13141 ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg gttcaggcgg aacagttcgt
    13201 cttcggtcag atagtttttg gcgattttgg cttcattcag cgtggggatt gcgccttgaa
    13261 aggtggtcag ccccataaag tctttgctgc tgtcggcacg gctgtatatc agctcagcta
    13321 cggtttgccg gctggcggca tagtgcagtt tgttttgaac ggcggcaaaa aaggtttggc
    13381 tttcgctgct tttggggttg tagtcttggc tggttgcata taaatcaagc acttgccggt
    13441 atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt gagcagttct ttccaatagt
    13501 cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc aaagcctttg gtcagatatt
    13561 cgtccagccg ttcggttgcc cattggcgga attggatgcc ccgcgcggaa cggacgcggt
    13621 agccgacggc aataatcatg ggcagggaat aatgggcgat tttgcggttt accttgcgcc
    13681 cgttttcatt ttgaactgtc aactggaagt tgacagttgc cttctcttcc aattcttgct
    13741 ctgcaagaat ggttttaatg tgtttgctga tattttgttt ggtggtttgg tacagttctg
    13801 ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc ctgcaaggca aattgggcag
    13861 tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca atctctgcaa tcacttcgtc
    13921 aatttcacgc cgcagccgtt cgattttggc aacggtttcg ctgatttcgg cgttaagctg
    13981 tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca tagctgctga cggcgaggtt
    14041 gtagccgttg tctttgacgg tttgctgggc ggcgttttgg gcgatatgcg gcacatcggc
    14101 tttatcggcg aagagtttga cgatttcggc aatgtgttct tcggttaaga cgttgttgtt
    14161 ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg atgtcggtat tgtctttgtg
    14221 tttggacaaa accagtatat tgacgacgat gccggtgccg taaaagagat tgggcgcaag
    14281 ggcgatgacg gtttccacgt agttgccctc caccagatat tggcggattt tctgctctgc
    14341 gccgccgcga tagaaaatgc cggggaatga gacgatggcg gcgcggcctc tgccagaaag
    14401 gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg gatttcgggg cgagtacgcc
    14461 tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg ccgctgccta tccagttgat
    14521 ggaatagggc ggattggaaa cgacggcatc aaagggtttg ctgtctttga gtttgggatt
    14581 ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa ttgacgttgt gcagaaacat
    14641 attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc tgcccgaaga agccttcttc
    14701 gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc aggctgcccg agccgcaagc
    14761 tgggtcgtag attttgttta ctttctcctg cccgtgcacc gccagccgcg caatcagctt
    14821 ggatacgctt tgcggggtga aaaattcgcc gccggatttg cctgcgttgg cagcgtagtt
    14881 ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg tggtggtctt caaaactgcc
    14941 gaaatcgagt tccgccacgc cttttaggac ggcggcaagg cgtttgttct tgtcggcaac
    15001 ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca aacaggcctt taatgtcttg
    15061 ttcggacgga tagccggagg cggagctttc aatcgcggta aaaatttctt tcagcttggt
    15121 gttgagctct tcgttttgat gggcttcggc ggcaatattg caaaaaagct ggccggggta
    15181 gatgaaatag cctttgactt tgacggcatc gtctttgatt tcgggcgtga tgatgctgtc
    15241 tggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc atatagtcgg taaagttttc
    15301 gctgataaag cggtagaaaa gtgtgccgag aacgtattgt ttaaagtccc agccgtccac
    15361 cgcgccgcgt acttcgtcgg caattttcca aatttggcgg tgcagttggg cgcgttgttg
    15421 catttcggtc atcatcgaaa tccatataaa agttaaacaa atcgaaatcg cctgatattt
    15481 tcagacgatt ttttacgggc attcaaatta agccaaattt tccagcatcc atttgacgta
    15541 gcgctcgacg cctgatttga catcgaaaaa ttcttccgta taacctgcct cacgcaattt
    15601 ggtgatgtcg gcttgagtga agctttggta tttgcctttg agcgcatcgg gaaatgggat
    15661 gtagcggata agttcttctt ctaccagctc tttcaagctc atttcgggtt tgccttcggc
    15721 ggcgcggcag gcgttgacgg tggcggcggc gagttcgtta aactgttggc tgcggccggt
    15781 gccgaggttg tagatgccgg aaagttcggg atggtcgaag aagtagaggt tgactttggc
    15841 aacgtcttcg acgctgacaa agtcgcgggt ttgttcgccg ttgccgtagc cgtcgttgct
    15901 gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg aaatggtgga aggcgacgga
    15961 tgccatacgg cctttgtgtt gttcgtgttg tccgtacacg ttgaagtagc ggaagccgac
    16021 gacttgggcg gtcaagcctt ctttcatgcg gcggcgcaat acttggtcaa acaggaattt
    16081 ggaatagccg tacacattga gcggtttttc gagttcgcgc tcttcacgga agatttcgcc
    16141 tttaccatac acggcggcgc tggaggcgta gaggaaggga atgcgttcgt cctgacacca
    16201 gtccaacaaa tccagcgtgt actggtagtt gttttccatc atatacaaac cgtcgtgatt
    16261 catggtgtcg gaacacgcgc cttgatggaa aacggcttcg atgttttgat aaggtaaaat
    16321 gtgttccctc acttggcgga tgaattcatg tttgtcgaga taatgggcga tttcgcactc
    16381 ggcaaggttt ttgaattttt cgcctttgct caaattatcg acggcaacaa tgtcagtaat
    16441 accgcgttga ttaagtgctt tgacgatgtt gctgccgata aagccggccg cgcctgttac
    16501 gatgatggtc atattcggtt tcctttgttt tgagtaaaat taataaactt agttacaaga
    16561 aatcatcgat attgtcctga aacacaaatt tgcgatgata ttccaaatat ttttctcttc
    16621 cttcgggata tattctcatc ttaatttttt gcatttggat tgatattaag tttttcccat
    16681 gtgtaacggc ttagtcgagt accgcaaatc aaacgaccat catccaagaa agtaatataa
    16741 ccttgatcga acaaccaatc ataagtcggc gttaaagcta acccatttaa ataatctaat
    16801 gcctctttct catttttctc agtaatacaa accatataag gtttaatatg actagccctt
    16861 aacaaaatct catctgtaat taatgtaaat ggacattgcg gcatatgatt tataacatcc
    16921 ttgcgatatt tctgctgccc ttttctactt ttctgtttaa tgatttcttg ctctacgaat
    16981 tgattaaatt ctgaagccgg tatttctaat ttttctcttg ataagagttg cgggtgcacg
    17041 atagagcgaa attgataatc caaaaatatt cggaaataaa ataatggttc tgaatcttct
    17101 atatctttga caggcaatag tttaagaatt gataaataaa taattttggg caatacaatc
    17161 ttacgccaaa ttgaccatat atcatcctcc gaacggatat aacccctacg gtaaccttta
    17221 gaattttcca aatggtctga tacatcatgg attgaaaaga aaatattttc tgtaggcaga
    17281 ttattaatac ttaaaacgta atcatcataa aaccttgaaa tatcatgatg atatttctgt
    17341 tcttgtttgt gatattcaac ttttgcatca accaaataac gctgtaaatt tttcttatta
    17401 aaaaaacaat ttttctgaat gaaaggatat tctaccctcc ttccattttt tgtctcagta
    17461 atcccccaat tttcatactc aaaaaatgta cttagttttc gaccactatc atcaatataa
    17521 ctacctacat atttgcgtgc ttccccatta ccaccaaaaa cagataactt gttattcctg
    17581 tgaataaaac tatcttccgc acgtaaattt tgaatactat cgacaataaa atactctatg
    17641 ttttcaatag taattctttg aaaatccggc attttactct ccataaagcg taatatccat
    17701 ttgttttaat atagcgatta atacatcaac aacaatactg tttcccgctt ggcgatacat
    17761 ttgagtatca ctaactaaaa ttttaaaatc atctctaaaa cccattagcc gcaaacattc
    17821 cctaggtgtt aatttacgaa tacgtccacg atcatgagta acataattat caaccccggc
    17881 acgatgcatt ttatgcatag tttgcaataa tgggcgagct acaggcaaat cagtttccgt
    17941 actggtttta aaattttttg ttcctcctgc caaaacataa tttttgattt tttcagaaag
    18001 ataatatttt tcctcaacgt tatttacatc aaaaataaag tcatcaaatt cagattcgcg
    18061 ggctgcctga aaaataaaat caccatgcca attaaattgt tgatttgctt tttggcataa
    18121 ggcaatttct ccattaatct gtgtataacg tttttgtcta tttttagaac tggtaacaaa
    18181 tttcgcaccc ttttcacgta aaaaatattt gctatcagta tagtcctcca aaaaatcttg
    18241 catagtatgt tctaattcaa tcttttcagg aaactgaaaa ccatttatag gaggggtatg
    18301 aaagccaaca acaaaaatac gctcacgatg ttgaggaatc ccataatcct tactattcat
    18361 tatttggaaa tataagtcat aaccaagtga ataaaaaaca ctttttacaa ctttccaagt
    18421 ttttccatta tcatgattga gcaagccctt tacattttca taaataaata tctttggttg
    18481 gacttcatcc acaacgcgag caaattcata gaataatgtt cctcgtgtat cttctaatcc
    18541 tgcacgtttg ccaaccatgg aaaatgcttg gcaaggactg cctccaacta aaatatcaac
    18601 ttgatttcta aactttctcg catcaaattg agtaatgtcg ttatgccaaa aatcttcatt
    18661 taatttatag tttccaagat aactttttta acgtatggat caatatctcc tgcaaaaaca
    18721 atggtatggt ttaaatttaa tcggtgaaat gcctgttcaa ccgcaccaat cccgctgaat
    18781 acggttgcta atctaatatg tgaatcaggt ttaagaaaag ttttagattg ccaaccttgt
    18841 tgactgggaa agagcaaagt tttttgtaat cgagtatcgt gtgtctgtgc cattgtcgaa
    18901 atagtcatac ttatatcgtt ctgtttatct tatcaatatg aaaactacat tgttgattgc
    18961 cctgacaatg ccttggtcaa ttcggcaaac gagcaaaccg ccgtaccgag tttcgccacg
    19021 acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg gcatggtgta gcctgccgcc
    19081 agccccaaac ccactccggc aataacggtg tcgcccgcac cggatacgtc gtaaacttct
    19141 tgggcgcggg tgggctggta aatcggttcg ccttcactga acaaggtcat gccttcttcg
    19201 cttcgggtca gcaaaatcgc ggtcaaatcg aggtggcggc gcaggttttg cgctttttcg
    19261 gtcagctcgc cttcgttttt ccaactgccg accacttctt tcaattcggc gcggttgggc
    19321 gtaatcagag ttgcgccgac atatttttcg taatcgtcgc ctttggggtc gattaatacg
    19381 gttttgcctt cgtgtttcgc ccaatcgatc atatcggaga tgtgcgacag gccgcctttg
    19441 ccgtagtctg aaaaaatgat tgcgtcgtat tcgggcaaga tttcgcggta tttccgcttg
    19501 atttgttcca acacttcgcg gttgggatgt tcttcaaaat caaggcggat aagctgctgg
    19561 ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt gtttgtcgcg catcagatag
    19621 gaggcaacgc cgtcctgcac catcagcgcg tcgagcgcgt cggcggcttc gtcgtcgccg
    19681 gttaccgaca acagccctgc cctgccgccc aacgaagcga tgttgcgcgc aacgttcgcc
    19741 gcaccgcccg cgcgttggtc gatacgtccg atttttgcca ccggcacggg ggcttcgggt
    19801 gaaatacggg acacgtcgcc aaaccaatag cggtcgagca tcacgtcgcc aacaaccaga
    19861 actttggctt gtgcgaaacg ggagttgagg gtttcttgtt gaaacttggc gggcatattg
    19921 cctccgttgt gttttgttca gacgacctta accggtaaga ccgcctgaaa atcagtttgc
    19981 ttcaaggttt gccacgcggt tcacttcgcg caatacggct agcggatcgg cagcttgggt
    20041 aacgggacgg cccatcacga gataagtcga acctgccgcc aaggcttcgg cgggcgtcat
    20101 gatgcggcgt tggtcgtcgt tgttgccggc aacgtccaag cggataccgg gcgtgaccaa
    20161 gacaaaatct tgtcccaatt cgcggcgcag cggtgcggct tcttgggcgg agcagaccac
    20221 gccgtccaag cccgaacttt gcgccagttt cgccaaacgg atgacttgtt cttcgggagc
    20281 gatgttcaaa ccgatttccg ccaaatcgct ttgttccatg ctggtcaaca cggttacgcc
    20341 gattaagagc ggctttgtgc cgtatccggc aacggcttct gctgcggctt ccatcatacg
    20401 gcggccgccc gacgcgtgca tatcgaccat ccaaacgccc atgtcggcag cgactttgca
    20461 ggcttgcgcg acggtgtggg gaatatcgtg gtatttcaaa tcgaggaaaa gtttgaaacc
    20521 ttgattgatt aagctttctg ccaaactgcg tcccgtcgcg gtaaacagct ctttgccgat
    20581 tttgatttga cacaatgtcg ggtcaaggtt gcggacgaat ccgagcgtgt ctttttcgtt
    20641 ggcaaaatca agggcgacga tgacgggggt gcgttgttgc ggagtttgga agtcggagat
    20701 taaaggattc atggtgtttt cgtttcgggt tttcagacga cctttatcgt ctttttcgca
    20761 gtatagtgga ttaactttaa accggtacgg cgttgtctcg ccttagttca aagagaacgc
    20821 ttctctaagg tgctgaagca caaagtgaat cggttccgta ctatctgtac tgcctgcggc
    20881 ttcgtcgcct tgtcctgatt tttgttaatc cactataaca acgtaaattc aaaccgtagg
    20941 tcgggcattg atgtccgacc tacgcggact caagcctccg acaaactgtt
//
LOCUS       DAXIEB010000024        20480 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000024 DAXIEB010000000
VERSION     DAXIEB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.024"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..253
                     /locus_tag="ACFOA3_000751"
     CDS             <1..253
                     /locus_tag="ACFOA3_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6897785.1"
                     /translation="ETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFYQRLPSEG
                     KPYKVAVTACMRKLLTISNARMRDYFAENGITENGIRTA"
     gene            301..867
                     /locus_tag="ACFOA3_000752"
                     /pseudo
     CDS             301..867
                     /locus_tag="ACFOA3_000752"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            947..1375
                     /locus_tag="ACFOA3_000753"
     CDS             947..1375
                     /locus_tag="ACFOA3_000753"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6897786.1"
                     /translation="MPSCANCTITNSFANTTSALYYLLQLFAAAGYLWTGAAKLQNLP
                     ASAPLHLITLGGMTGGVMMVWLTAGLWHSGFTKLDYPKLCRIAIPILFAAAVSRAVLM
                     NVNPIFFITIPAILTASVFVLYLFTFVPIFRANAFTDDPE"
     gene            complement(1422..3611)
                     /locus_tag="ACFOA3_000754"
     CDS             complement(1422..3611)
                     /locus_tag="ACFOA3_000754"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6897787.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWTEQGWIETAEAAKPVLRSCHGQASHAGFVLNAGQQKASDEIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVEIL
                     NAGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQIAVRADALRVADAMEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQET"
     gene            3750..4532
                     /locus_tag="ACFOA3_000755"
     CDS             3750..4532
                     /locus_tag="ACFOA3_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6897788.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4945..6879)
                     /locus_tag="ACFOA3_000756"
     CDS             complement(4945..6879)
                     /locus_tag="ACFOA3_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6897789.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6945..8123)
                     /locus_tag="ACFOA3_000757"
     CDS             complement(6945..8123)
                     /locus_tag="ACFOA3_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6897790.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQASYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8349..8738
                     /locus_tag="ACFOA3_000758"
     CDS             8349..8738
                     /locus_tag="ACFOA3_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6897791.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYNQAKLKQNKYFNITLSKVSPDHFTLKATPNPTTNE
                     GETCIVTLNDGGTLSAAGNGQSCPDFD"
     gene            9022..10068
                     /gene="adhP"
                     /locus_tag="ACFOA3_000759"
     CDS             9022..10068
                     /gene="adhP"
                     /locus_tag="ACFOA3_000759"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6897792.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGDKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVDCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10144..13629)
                     /gene="smc"
                     /locus_tag="ACFOA3_000760"
     CDS             complement(10144..13629)
                     /gene="smc"
                     /locus_tag="ACFOA3_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6897793.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIYVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TTEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTRQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQASDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPYGFVPPTPLPQGQAAWL
                     SDGLSSGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKAHLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIFTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLTQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            14080..15234
                     /locus_tag="ACFOA3_000761"
     CDS             14080..15234
                     /locus_tag="ACFOA3_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC6897794.1"
                     /translation="MGLGNIAIGVNAESQGNDISYATSIGTFAKANKSAALALGGASE
                     ALGIYSIAEGYSANSDGYGAIAIGSAAKIKPLEKGKINHIAGNNNKGLDVDADGNVTK
                     ITITTESEKDILSKYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESVAVGDSSQS
                     TGYRSATFGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRK
                     IVNVADGAYDFDAVNVRQLNRLSKRANRIGASAAALASLKPAQLGKNDKFAFSLGFGS
                     YKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKPTPAVAAQNAAHS
                     AEVSQLRQEVAALRAQQAETDRKLHKQAEMENELQQLRRTLSELKKTLKQ"
     gene            15434..16156
                     /gene="lpxH"
                     /locus_tag="ACFOA3_000762"
     CDS             15434..16156
                     /gene="lpxH"
                     /locus_tag="ACFOA3_000762"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6897795.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQAREIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     GSYASILRVDGDGAVFVPLEKC"
     gene            16424..18010
                     /locus_tag="ACFOA3_000763"
     CDS             16424..18010
                     /locus_tag="ACFOA3_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6897796.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVTAMIFFL"
     gene            complement(18178..18381)
                     /locus_tag="ACFOA3_000764"
     CDS             complement(18178..18381)
                     /locus_tag="ACFOA3_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6897797.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            18497..18994
                     /locus_tag="ACFOA3_000765"
     CDS             18497..18994
                     /locus_tag="ACFOA3_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6897798.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLVAEHIRSNWETFA
                     HIGQA"
     gene            19076..20269
                     /locus_tag="ACFOA3_000766"
     CDS             19076..20269
                     /locus_tag="ACFOA3_000766"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6897799.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLDAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLNRAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
BASE COUNT         4481 a         5814 c         5381 g         4804 t
ORIGIN      
        1 ggaaaccaaa ttcaaaagcc gctgctttgg cggaaggtct gcggtgctta aggcactgta
       61 tatggctacc gtggcagcga cacgttttga accgcttatt cgggatttct accaacgcct
      121 gccgtccgag ggtaagccgt ataaggttgc cgttacggca tgtatgcgca aactgctgac
      181 gatatcgaat gcccggatgc gtgattattt tgccgaaaac ggtatcaccg aaaacggtat
      241 ccggacggct tgatttgagt tttggtattt ttacccggcg gggtgaaaaa tactgttgct
      301 atgcgcccgt ttttcgtcgg cgcgacagtg cttgccatac tcggcgcgtt ggtgtttttc
      361 atcagccccg atgccgtcgt cctgcaccgc caaatcttct tagaactcat gctacctgcg
      421 gcatacggcg gttttttgac tgccgctttg ttggactgga cgggtttttc gggcaacctg
      481 aaacctgtcg cgactttgat ggcggcatta ttgcttgtta cggctgtttt attgccgttt
      541 ttaccgcaac ttgccgcatt tttcgtcgcc gcctattggc tggtgttgct gctgttctgc
      601 gcacggctga tttggctaga ccgaaacacc gacaacttcg ccctcttaat gctgcttgcc
      661 gcgttcaccg tttttcagac ggcatacgct gcaaccggcg atttgaacct gttgcgcgcg
      721 caagtgcatt tgaatatggc ggcggtgatg ttcgtatccg tgcgcgtcag tattcttttg
      781 ggcacggaag ccctgaaaga atgccgtctg aaagaccctg tttttattcc aaatatcgtt
      841 tataaaaaca tcgccattac tttcctgctc ttgcacgccg ccgccgaact ttggctgcct
      901 gctcaaaccg ccggttttac tgcgcttgcc gtcggcttca tcctgcttgc caagctgcgc
      961 gaactgcacc atcacgaact ccttcgcaaa cactacgtcc gcactatatt acctgctcca
     1021 actttttgcc gccgcaggct atttgtggac aggcgcggcg aaactgcaaa acctgcccgc
     1081 ctccgcgccc ctgcacctga ttaccctcgg cggtatgacg ggcggcgtga tgatggtgtg
     1141 gctgactgcc ggactgtggc acagcggctt taccaaactt gactacccca aactctgccg
     1201 catcgccatc cccattcttt tcgccgccgc cgtttcgcgc gctgttttaa tgaacgtgaa
     1261 cccgatattc ttcatcacca tccccgcgat tctgaccgcc tccgtgtttg tgctttatct
     1321 ctttacgttc gtacctattt ttcgggcgaa cgcgtttaca gacgatccgg aataaaaatg
     1381 ccgtctgaaa cggttttcag acggcatcag attgccaata atcaagtctc ctgcggatcg
     1441 acatccaccg accatctgat tttgccgtcg cggttttgct gcaacacctg cacccacaaa
     1501 ctcacggcgc ggtgcaaatc ctgtcgagat gtcgattcga ggaaaacttg cgcacgttcg
     1561 cgttcggcaa ggcgcaccat cagcatgggg gcggcgccga actgggaaac gcttgcgggc
     1621 aaaagcgggg cgagggtttc tttggcggcg ttgagaaatt ccatcgcatc ggcaacacgc
     1681 agcgcatcgg cgcggacagc aatctgaaaa ccaaagggcg gcatggcgaa catttgccgc
     1741 tcgttcaatt cgttttcggc gaacacagcg tagtcctgcg ctttgacggc ggcgaagacg
     1801 ggatgttcgg gcagttgggt ctgtatcaac accttgccgg gtttgtcggc acgccccgcc
     1861 ctgccggaca cttgcatcag ctcggcaaac agcctttctg gcgcgcgaaa gtccgcgctg
     1921 tacaggctgc cgtcggcgtt caacacgata acaaggttga gccgcgcaaa atcatggcct
     1981 ttggcgagca tctgcgtgcc gaccagaatg tcgatttcgt tgtcggcgat gcggcggtac
     2041 aaatccgccc agtcattttt gtgcgcggtg ctgtccctgt cgacgcggac gacggcggcc
     2101 ttgggcagga aggcacgcag ggtttcttcg acgcgctgcg tgccgtggcc gacggcggtc
     2161 aggtcttgat tgccgcagtc ggggcatttg tacgggatgg gttcgcggtg gtcgcagtgg
     2221 tggcagcgca gttggcgggc gcgttggtgc agcaccattt tggcggagca gttcgggcag
     2281 ccgaaggtat ggccgcagtc gccgcaaaac agcgcgggcg caaagccgcg ccggttgagg
     2341 tacaccagcg acataccgcc tgcttcaaaa ttctgtttca gaagctgcaa ggcttgcggc
     2401 gagaaaccgt tgtcgagttt cagacggcct gcgttgagga tttctacttg cggcagttgc
     2461 gcggcggcat gggcgcgttc ggtcagttgc aagaggcggt acgcgccgct ttgtgccttg
     2521 tgccagcttt ccaagcttgg ggtcgcgctg cccaacacga cgggacagcc gccctgcttc
     2581 gcccgccaca ccgccaaatc gcgggcgtgg tagcgcaatt cgttgtcctg tttgaacgag
     2641 ccgtcgtgtt cctcatcgac cacaatcagc ccgacatcat ccatcggcgt gaacaccgcc
     2701 agccgcgtgc cgatcaccag cttcgcctgc cccgacatcg cgcgcagata atcctgcgtt
     2761 cgcctgcctg ccgccatccg gctgtgcaac acggcggtcg gcacgtcggc aaaacggttt
     2821 tccacccgct tcaaaagctg cggcgtaaga ttaatttcgg gcaacagaaa caacacctgc
     2881 cgcccctgcg ccaacacttt cgccatcgca tcaaaataca cctcggtctt gccgctgccg
     2941 gtgatgccgt acagcaaaaa cggctgaaac cgtcccaatg ccgtctgaat ctcatcggaa
     3001 gccttctgct ggccggcgtt caacacaaat ccggcgtgcg aagcctgccc gtggcacgac
     3061 cttaaaaccg gtttcgccgc ttccgccgtt tcaatccagc cctgctccgt ccaatcttcg
     3121 attaatttcg ccgcctgcgc gttcacctgc ttcaacgctg ccatcgtcat tccgcccgaa
     3181 agcagcgcgt cccacaaagc cgcttttttg ttgaaccgcg ccggcggcgg cgtttgcgcc
     3241 ctgcccgctt cgttcaacgc ataaaacaac ggcggctgcg gcatttccac cgcgcgcgtt
     3301 tccttcaaac cctgcggcag cgcggcaaac accgcctgcc ccgtcggata gtggtaataa
     3361 cgcgacgtaa acgagagcaa atcacgccag ctttcaggca acggcggctc gtctaaaaag
     3421 gtcgtctgaa tactcaaaat ccgcgccgta tccatatccg gcgcaatatc cgtttcccac
     3481 accatcccga ccacggtttt attgcggaaa ggcacaagca cccgcgttcc cggaggaagc
     3541 ggatcggaat gggaataagt caaaaggccg tctgaaagcg gcacgtttac ggcgatgcgg
     3601 tggtagatca tacaggcagg caaatcattt ttttgcgtat tttaacaggc ggaagctatt
     3661 ttcagacgat aaattgagcc taggcgcaat gcaggcgcaa cagagatttg atacaattcc
     3721 gcctttccaa cacgcgcaat catctttata tgaaaaccaa gttaatcaaa atcttgaccc
     3781 cctttaccgt cctcccgctg ctggcttgcg ggcaaacgcc cgtttccaat gccaacgccg
     3841 aacccgccgc caaagccgag tccgccggca aatccgttgc cgcctctttg aaagcgcgtt
     3901 tggaaaaaac ctattccgcc caagatttga aagtgttgag cgttagcgaa acaccggtca
     3961 aaggcattta cgaagtcgtc gtcagcggca gacagattat ctacaccgat gccgaaggcg
     4021 gctatatgtt cgtcggcgaa ctcatcaaca tcgacacgcg caaaaacctg accgaagaac
     4081 gcgccgccga tttgaacaaa atcgacttcg cctccctgcc tttggacaaa gccatcaaag
     4141 aagtgcgcgg caacggcaag ctgaaagtcg ccgtcttctc cgaccccgat tgtccgttct
     4201 gcaaacgctt ggaacacgag tttgaaaaaa tgaccgacgt gacggtttac agctttatga
     4261 tgcccattgc cggcctgcac cccgatgccg cgcgcaaggc gcaaatctta tggtgtcagc
     4321 ccgaccgcgc caaagcgtgg acggattgga tgcgtaaagg caaattcccg gtcggcggca
     4381 gcatctgcga caatcccgtc gcggaaacca cttccttggg cgaacaattc ggcttcaacg
     4441 gcacgccgac cctcgtcttc cccaacgggc gcagccaaag cggctacagc ccgatgcccc
     4501 aactggagga aatcatccgc aaaaatcaat aatccatccg caaaaaaggc ttggaaaatt
     4561 ccaagccttt tttgcgggaa atgccgcctg aaaaccaatc cgccctgaaa gttagcaagc
     4621 caacaaacac atcctaaccc attgaatgat tgaaaataat aaattgaaca atacgaagcc
     4681 ccgccgccgt tcccacgcgg gcgggaatct ggaatattga gttaccgtga tttatccgaa
     4741 acaagcaaag ccgaacgaac cgaattcccg tctttacggg aatgacggga ctcgagcgtt
     4801 gtgtgcaatc atcgaacccc cgccgccgtc attcccacgc aagtgggaat ctagaacgta
     4861 gaatctaaga aaccgttttg tccgataagt ttctgctcca aaatgaccgg tccggcagct
     4921 tcaaacggca tctttgtgcc aacctcaatc ctgcgccaat gcgtctatcg gattgagttt
     4981 ggctgcttta ttggcaggca taaagccgaa cgcgatgccg attccggtcg agcaggcgac
     5041 cgccccgata acggatgccg ccgaaatgtc catcgggaag tcggttacaa aatgattgaa
     5101 cacgaggctg acggcggcgg acaaacccac gccgaccaaa ccgccgatga cgcagattaa
     5161 caccgcctca atcaaaaact gctgcaaaat attgccgcgc cgcgcgccga ttgccatccg
     5221 tatgccgatt tctttggtgc gctcggtaac ggacaccagc atgatgttca tcacgccgat
     5281 gccgccgact accaatgaaa tcagggcgat ggaggaaatc agcagcttca tcgtaccggt
     5341 ggtgctttcg actatctgcc tgatgctgtc gctgttgttc atgaagaaat cttccgtgcc
     5401 gtgccgcgct ttgagcagat cggtcagccc tttttcggca acctgggtat tggcattgtc
     5461 tttgattttg acggtgatgg agttggtgtg gctctcgcct gtgatttggt gcatcaccgt
     5521 cgtatagggc gaccaaagca tcagcacgtc ggaattgccg aaagcgtttt cgtctttttt
     5581 catcacgccg atgacggtca aggggcgttt cctgaacaaa atggttttac ccaacggatc
     5641 cgagtccgca aagagtttgt ctttgacatt ttggtcgatg acgacgacct gcgcgtcttc
     5701 tttcacatcg ttttcgtcaa acagccgccc cgtttccagc ttcagtccgc gcacgtcgaa
     5761 atattgttcg cccacgccgt acaaagaagc ggtcaggtcg gtattgcggt aagtcagcgt
     5821 gccgccgctc gaagtcatgg gcgtggcgga agcaacgtag ctttgtttgg cgatgatttt
     5881 tgcgtcgtct atggtcaggg ttttaatcct gccgctgcgc ctgtcgccga agccgcgccc
     5941 tgggaagatg ctgatggtgt tcgtccctat cgaactgatg tcttcaagga tttttttctg
     6001 cgaaccgttg cccaatgcga cgacggagac aaccgaagcg ataccgatga tgatgccgag
     6061 catcgtcaga agcgaacgca ttttgtgcgc caatactgct tgcaccgaca ttctgaaggc
     6121 ttcgacaaac tggtcgtaat aaaacgacca cgaggctttt tcccgaatcc tcccgacatt
     6181 gcttgcgggg atttcgggat ttttcgaggt gtcggaaatg atttcgccgt cccggatttc
     6241 gatgacgcgg ttggcattgg cggcgatgcc ggggtcgtgc gtgaccataa tgacagtatg
     6301 cccggcttcg tgcagcttgt ggatgatttc catcacgttt ttgccgctgg cggtatcgag
     6361 tgcgccggtc ggctcgtcgg cgaagatgat ttctccgccg ttcatcaggg cgcgggcgat
     6421 ggagacgcgc tgctgctgtc cgcccgagag ttcgccgggc ttgttgccct ctttgcttgc
     6481 caaacccaaa tcctgtaaca acttctccgc ccgcgcggaa cgctctttgc cgcccgcgcc
     6541 catatagacg gctggcagcg cgacgttatc ccttgcggtc agcgagctta agaggttgta
     6601 gcgttggaag atgaaaccga agcgttcgcg gcgcaatgcc gccagctcgt caggctgcat
     6661 tttggcagtt tcgatgccgt cgattcggta cgaaccggaa ccggcggtat ccaaacagcc
     6721 gaggatgttc atcagcgtgg actttcccga accggactgc ccgatgatgg cgacaaaatc
     6781 gcccttctct atcgacaggc tgatgtcttt caaaatatgg acgcggttct cgccgctgcc
     6841 gaaatagcgg ttgatgtttt tacattcgat caagctcata atattccgta aatcaataac
     6901 aaatgccgtc tgaaaccgtt tccgtgttca gacggcatat tcgtttatcg gcgcggcggg
     6961 ccgcctaggg cgcgttcgcc gctttcctgc tgctcggcgg cggttatttc ggagatgacc
     7021 actttgtccc cctctttcaa cccgcttttt acttcggtat tcatactgtc tctcataccg
     7081 gtccggattt cgcgttccgc cgccttgccg tccgcaccca acacgcgcac aaacgccttg
     7141 ccgccgcgat ttttcacggt cagcgacgga ataatcagca catttttcac gccgtcgatt
     7201 tcaaccgtat tctgcgtcgt catccccgtg gcgagtttgc cgtccggatt cggcacaaac
     7261 gaacgggcat aatagtagac cgcattggaa gccgtatccg tactgctgtt gtagccgccc
     7321 gacgacatcg tggtcagccc ggggtcgacg ctgtcgagct tcgccttaat cggcgtatcc
     7381 ggttcggaca aaatcgtaaa cgaaatatcc tgccccgcct tcaccttggt aatatcgccc
     7441 tcggcaatct gcattttgtt caacatcata tccagattcg ccaattggac aatcgtcggc
     7501 gtagactgcg ccgcgttcac agtctgcccc tcttccacga gaatcgccac caccgtgccg
     7561 tccatcgttg cggtaatgcg cgtgtagccc aattccgact cggcggtatt gatggaaatt
     7621 ttgctctgtc tgattagagc cttcagctcg gcaacattgg ctttggcggc ggcaagcgca
     7681 tcctgtgcgc tttccaaatc ttctttagcg gtcgcatcat ccttccacaa cgccgcctga
     7741 cgcttatatt tcttctccgc gctgcccaat gcaatctgtg ccgacaccag cttcgcctga
     7801 tacgtttcca atttggattt ttccgtattg agcgtattgg tctgcgaggt cgaattgatt
     7861 tccgcaatca aatcgccctt tttaacctgt tgcccgagtt tgacataaag tttcttaatc
     7921 tgccccgatg cctgcgcgcc gaccgatacc aggttggacg gcgaaatctc ccctgttgca
     7981 gaaaccgtcc ggctgatgtc gccgcgcctg accgtttccg taatataaga agcctgcggt
     8041 tcgggcttca gataagacca tccgccccaa accgctgccg ccgcgaccgc cgcaacagcc
     8101 gcccatttca tcatttttgc catgttttca attctctgta tgcgtcaatc ccataaatcg
     8161 gaagcggatt ctaccgcaag tccctgcccc atccaaggcg gcaaactgcc gacaataaaa
     8221 ataaccattt gttttaaatg gcttatacaa tctactttcc cgaaaatttc aatccgttta
     8281 catattccgc catttccgat acggcgttat aatgccgtcg accgacaaac aaatggaaac
     8341 acattgctat gaaaaacgtt caaaaaggct ttacgctgct cgagctgatg attgccgtcg
     8401 ccatcctcgg catcctgacg ctcatcacct acccttccta taaaacctac atccggcgcg
     8461 tccgcctgtc ggaagtcaaa tccaccctgc tgatgaacgc gcaaaacttg gagcgttact
     8521 accgccaaaa agggacgttt gacaactaca accaagccaa actgaaacaa aacaaatatt
     8581 tcaacattac cttaagcaaa gtcagccccg accatttcac ccttaaggca acgcccaatc
     8641 ccaccaccaa cgaaggcgaa acctgcatcg tcacgctcaa cgacggcggc acgctttccg
     8701 ccgccggcaa cggacaatcc tgcccggact tcgactgatc ctccgccgaa cccgcaaacc
     8761 cgtaaaaatg ccgtctgaag ctgtgttcag acggcatttt tgcgggtttc ccgtccggtt
     8821 ttttcaaact tgaaacctgc cgccccttat ccggcaaccg ccccgatgcc gcgcctttcc
     8881 aggttgttat agtataacat ataatccata tttttcaaca acataccatt tgacccgcaa
     8941 gatgcggaag cgtatgctta cccttatatc ggacaacatt ccgtttacag gtttttttta
     9001 ccgtaactaa ggagcgcaat catgaaaatg caggcagttg ttgtgaataa aaatgtagcg
     9061 ggcgatgtgg aagtaatcga acgcgaggtt cgcccgttgg aatacggcga ggcgttggtc
     9121 gaagtcgaat attgcggcgt gtgccacacc gacctgcacg ttgcggcagg cgactacggc
     9181 gacaaaccgg gccgcgtgct gggacacgag ggcatcggtt tggttaaaga agttgccgac
     9241 ggtgtgaaaa atctgaaagt cggcgaccgc gtcagcatcg cctggctgtt ccaaagctgc
     9301 ggctcgtgcg aatactgcaa taccggccgc gaaaccctgt gccgttccgt attgaacgcg
     9361 ggctacaccg ccgacggcgg tatggcgacc cactgcatcg tgagtgccga ttacgcggtc
     9421 aaagtccctg aaggtttgga tcctgcgcaa gcttcgagca ttacttgtgc cggcgtgacc
     9481 acttataaag ccattaaagt ttccggcgtt cgtccgggac agtggattgc catctacggc
     9541 gcgggcggtt tgggcaactt gggggtccaa tacgcgaaaa aagtattcgg cgcgcacgtt
     9601 gtcgccatcg acatcaacga cgacaaactg gcgtttgcca aagaaaccgg cgcggatttg
     9661 gttgtcaacg ccgccaaaga agacgctgcc aaagtgattc aggaaaaaac cggcggcgca
     9721 cacgctgcgg tcgtaaccgc cgtatctgct gccgcattca actctgccgt ggattgcgtc
     9781 cgcgcgggcg gacgtgtggt tgccatcgga ctgccgccgg aatcgatgga tttgtccatc
     9841 ccgcgtttgg ttttggacgg catcgaagtg gtcggctctt tggtcggcac gcgcaaagat
     9901 ttggaagaag ccttccaatt cggcgcggaa ggtttggttg tgccgaaagt ccaactgcgt
     9961 gctttggatg aagcacccgc cattttccaa gaaatgcgcg aaggcaaaat caccggccgt
    10021 atggtgatcg atatgaaaaa agaatgcggc tgcggtcatc accactgatt tgacgtggcg
    10081 atacacatcg aaatgccgtc tgaacgctgt tcagacggca tttttgacat cccgatacgc
    10141 ctttcaaacg gcttccgcca tttccaatgc ctgcttgatg tccacggcga cgacgcgcga
    10201 tacgcctttt tcctgcatgg ttacgccgac cagctgctct gccatttcca tggtcaggcg
    10261 gttgtgagag atgtagagga actgggtttg cgccgacatt tctttgacca ggttgcagaa
    10321 acgcgaggtg ttggcgtcgt ccagcggggc atcgacttcg tccaaaaggc agaagggggc
    10381 ggggttgagg ctgaacaggg cgaacacgag gctcatggcg gtgagggctt tttcgccgcc
    10441 ggagaggagg tggatggtgc tgtttttctt gccgggcggg cgcgccatga tagacacgcc
    10501 ggcggtcaaa aggtcgtcgc ctatcatttt gagggtggct tcgccgccgc cgaacagggt
    10561 cgggaagaaa gtttggactt tgccgttgac agcatcgaag gtttctttga aacgcgcttt
    10621 ggttttgtca tcaatttggg cgatggcttc ctccaaaagt gcaatggcgg cctgcacgtc
    10681 ttcgctctgg ctgcggtagt aaccgtcgcg ttcacgcgct tcttcgagtt cttgcagggc
    10741 ggcgaggttg accgcgccga gggcttcgat ttgctgggtg aggctgccga tgctgctgtt
    10801 caatactttc ggtgattctt tcgccaaggc ttcgagcgcg tccaaatcgg cggcgcgttc
    10861 ggtcaggttt tgatggtagc gtttggcgtt gatcagggct tcctgctgtt gcaacagggc
    10921 ggtttgggtg gcggcctgaa gctgcggcag cttggtttgc agggtttgca ctttagcgta
    10981 ttgctccctg ccctgctcct gaatctgcgc gagtttttct tgtacgacaa tatattcttc
    11041 gtccaaagtc tgtacggctt cgcttaattc ttcgagcttg atgtgctgct cgtcgttttg
    11101 gaactcggtt tcataggcga gggcaagctc ttgctggcgt tcctgccagt ctagggtttg
    11161 ctgttcaagc cgggcgattt gctgttggta gttttgtttt tgctggttga gcttgtggac
    11221 cgcgacttcg gcaagcccgt attggcggtt ggcttccaac agagcaagct gcgcctgttt
    11281 cagacggcct tgctgctctt ggcggctgtg cgcggtggtt tgctgttggt gttcgagttc
    11341 ggcggcggct tcctgcaaag taaagatgtc gtctgaaagc ccgtcggacg tgtgttgcaa
    11401 cacggtctgc tcttctgcca actgcgccag ttcgcgctcg atgtgttcgc ggcggatttg
    11461 cccttggttg gtgcgcgcca agagttcggc ggcgcgttgc tgcgcttggc tgtattggcg
    11521 cgtgtgctgc tgttgttgct gcatcaggtt tttgtgctgc acttcggacg agcgcacggc
    11581 ggcttccgcc tgtttgaacg cggcttcggc ggcggagagt tcgggggcta ggttgtccag
    11641 ttcggatgcg atgccgtcga ggtgcgcttt ttgggcaatc aggctttcct gcgcgggctt
    11701 ggcatagagc aggacgctga ccttatcgac ctgatgacct tcgggcgtga gccagatttg
    11761 gtgtgcgccc aaatcgtttt gatgcgcgag ggcatagctc aaatcgggcg cgcacaatac
    11821 gccgtcgagc cagtggtgca atgccgtctg aaacggcggc tgcgcttgga tttggttcag
    11881 caatgcctgt acgggcaggg attttttgat gccgctggag aggccgtctg aaagccatgc
    11941 cgcctgcccc tgcggcaaag gcgtgggggg aacgaagccg tacggtacgg cgcgggcgtg
    12001 caggcgttcg gcaaggatga cggacaaggc gtgttgccac tcggcgggcg cggtgatgtg
    12061 ttgccacagt tgcggcgcgg cggcgtggtc gcttgcctgc cagaaatcgg cggcttcctg
    12121 ctgttgcgac aggatttgcg acaacgcctg ctgctgcgcc tgcaaggtga tatgttgctg
    12181 cttcaggctt tggaagcggt ttgatgccgt ctgaaacgcc tcgcgggcag cgtgtaaggc
    12241 ttcttcggcg gcaatgattt gttcttcgta atgctcttgc cggctttgca acaaggcggc
    12301 ggcttcctgc gcggcggcgg tttcggcctc gtcgggcagg ttcagggctt ggttttcctg
    12361 tttcagacgg cctttgcgct cttcgtgctt ggcaacggtt tgttcggcat gggcaagctg
    12421 ctgctgcttc aacgccagtt cgcggcggat gcggtttgcc tcgtcctgcc gggtttggaa
    12481 ggcggcgttg agcgtggctt gggcttcttc caattcgggc agacgctctt cgtgttcggc
    12541 aacctgcatc gcccattccg ccaactcggt ttgcttttct tcgacctgca actcgttttc
    12601 ttcaagctgc acgcggattt gctgctgctc ttgatgaatg cgttgtaact gcgcctgcgc
    12661 tgcctgcttg tcgcgttcga tgcgttggtg caggttttgc tgatggcgga tttgttcttc
    12721 caaacgggca atctgctcgc gcaacacgcc gcgtttgttg cccagttcgt gtaccgcctg
    12781 ctgctgcgat tgttcggtgg tctgcaaggc gtgtacttcg tcgtttaacg cctgaacctg
    12841 cgcggcggtt tcgtcctgct gcgcctgcaa agactgatgc tgcgcggtcg ccttgtcggc
    12901 ggcggcaagc gattgctgcc attgggcgta atcgagcaaa tcctgctgtt ggttcaactg
    12961 cgcggtcagg gatttgtagc gttcggcggt ttccgcttgt ttttccagct tttccacctg
    13021 acgcgccaac tcgttctgca aatcgcccaa acgctgcaaa tgctcgcgcg tgtctttcag
    13081 acgaccttcc gtctccttgc ggcgttcctt atatttggac acgcccgccg cctcctcgat
    13141 ataggcgcgc aactcctccg gccgagcttc gatgatgcgc gaaatcatcc cctgctcgat
    13201 aacggcataa ccgcgcgcgc ccacgcccgt acccaaaaat aaatcggtaa tgtcgcggcg
    13261 gcgcacggtc tgattgttga tgaaataagt cgattcgccc tgacgcgtca gctgccgctt
    13321 gatactcacc tcggcatact gcccccacgc cccctgcaaa ctgtggtcgc tgttgtcaaa
    13381 caccagctcc accgaagccc tcggcgcagg acggcgcgtc gccgcaccgt taaaaatcac
    13441 gtcctgcata ctctcgccac gaagctgctt cgccgaagcc tcgcccaaca cccagcgcac
    13501 cgcgtcaatc acattcgact tgccgcagcc gttcggcccg ataaccgcga caagctgccc
    13561 cggcacataa atcgtggtcg ggtcggtaaa agatttgaag ccggagagtt tgatgtgggt
    13621 caggcgcata atggtcggaa aaaataaaaa agaagcatat tttaacggaa atctcgccgc
    13681 accaccccat atctttccgg caaaacctta ccgcatcccg ccccggtgcg gcaggatttt
    13741 gcacatcccg gccatccgta ttaaaatctt tgcgtttcca acactttcct gacaaatata
    13801 cggaggagga ttccatgaaa acagaaacag tcaagcaagc aagcaagcaa gcaagcaagc
    13861 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaaaatagt
    13921 ctaacccgcc tttctttaat taccctactt atttccgctt ctttttatcc tgttcagtct
    13981 gttgcggctg cccctcaaga aataaattat tcgttatcag gcggtaaagt ttacgcagat
    14041 aagttcagta tcgccatagg ggatagcgat accaaagcat tgggacttgg caatattgcc
    14101 ataggggtta atgcagagtc tcagggaaat gatattagtt acgcaacatc aatcggtaca
    14161 tttgctaaag caaataaatc agcggcattg gctctagggg gagcttctga agcattggga
    14221 atatacagca ttgcagaagg atatagtgcg aatagtgatg gctatggtgc aatagcgatt
    14281 ggttctgccg ccaagataaa gccgttggaa aaaggcaaaa taaatcatat tgcgggaaac
    14341 aataataaag gtcttgatgt cgatgcagac ggcaatgtaa caaagataac cataacgact
    14401 gagtcggaaa aagatattct ctcaaaatac ggtcaaactt acggtgcgat ggcactagga
    14461 tttagatctt cttcacacaa tctttttgcc agttcatttg gagcattttc tacagccaca
    14521 gctatcgaaa gtgtagcagt cggcgacagc agccaatcaa cgggctaccg cagtgctact
    14581 tttggcagtc acagtagggc tttggcagaa gaaagtttgg cattaggtta tgaaactcgg
    14641 gcaaatgctt atggttctgt cgctttaggc gcagaatctg tggcgaatga agaaaatacc
    14701 gtatcagtgg gttccgatac attgaaacgg aaaatcgtta atgtcgccga cggcgcatat
    14761 gatttcgatg ccgtcaatgt ccgccagttg aaccgcttaa gcaaacgtgc aaaccgcatc
    14821 ggcgcaagtg ctgcggcatt ggcttcgtta aaacctgcac aattaggcaa aaacgacaaa
    14881 ttcgcctttt ctttgggctt tggaagctat aaaaatgccc aagcggtggc aatgggggcg
    14941 gtgtttaagc ccgctgaaaa tgtgttgctt aatgtggcgg gcagttttgc cggaccggac
    15001 cgggctttcg gcgcgggggt ttcttggaaa ttcggcggca aaccgacacc tgcggttgcc
    15061 gcacaaaacg cggcgcattc tgcggaagtt tcgcaattgc ggcaggaagt ggcggcgttg
    15121 cgcgcccagc aggctgaaac cgaccgcaaa ctgcacaaac aggccgaaat ggaaaacgag
    15181 ctgcaacagt tgcgccgcac gttgtccgaa ttgaaaaaaa cattgaagca ataaacgtaa
    15241 ggcgggacgg cggctctgcc tttttccgat accgcacccg cgcgatgccg tctgaagcct
    15301 tcagacggca tttttaacgc gccgaacccc ggttccgcca atgcccgatg cttggcgcgg
    15361 catttgccgt attgccccgc cgccgggcgc gttataatcc gccacgacat ccgcccgatt
    15421 tggaaacctg cctatgaaac ccgcctattt catctccgac ctgcatttga gcgaaaagca
    15481 ccccgaactg accgcgctgc tgctgcgttt tttacgttct tccgccgccg ggcaggcgcg
    15541 ggagatttat attttgggcg atttgtttga tttttgggtg ggcgatgacg aagtttccga
    15601 gttgaatact tcggttgcgc gggaaatcag gaaattgtcc gacaaaggcg ttgccgtgtt
    15661 cttcgtcagg ggcaaccgcg acttcctgat cggtcaggat ttttgccggc aggcgggtat
    15721 gacgctgctg ccggattact cggttttgga cttgttcggc tgcaaaaccc tcatctgcca
    15781 cggcgacact ctgtgtacgg atgacagggc atacctgcgt ttccgccgca tcgtgcattg
    15841 caggcggctg caaaaactgt tcctgatgct gcccctgaaa tggcgcacgc gccttgccgc
    15901 caaaatcagg cgtgtcagca aaatggaaaa acaggtcaag cccgccgata ttatggatgt
    15961 caatgccgcc tttaccgcgc ggcaggttcg cgcctttaac gcggaaaggc tgatacacgg
    16021 acacacccac cgcgagcata tccatcacga aaacggcttt acccgcatcg ttttgggcga
    16081 ctggcacggc agttatgcct cgattctgcg tgtggacggg gacggcgcgg tattcgtgcc
    16141 gctggaaaaa tgctgaaaat gccgtctgaa gcctttcggg cggctttttt acacgcccct
    16201 ttcccacact ccctcctccg catcatcacc atccttaacc cgcattaata gccattatcg
    16261 ataaaatgct tgaccgatta cataagatta cgtaaagtgt gcaaacgcat aataattggt
    16321 cttaccaatt tatcttttaa ccaaaatatc gggtgtacgg gatttgaacg tctttccaaa
    16381 cctcaacgcg ccttatgatg caaatgccta aaaaggattc gttatggcac ttttcctcag
    16441 catattcccc atcgtcctgc tgatttggct gatggtgaaa aaaaacagta tgccttccta
    16501 cgtcgcactg ccgattaccg cagtgctgat ttacgccatc aaacttttct acttcggcga
    16561 tgcgggcatg ctgctcaacg ccaccgccgc ttccggcctc gtcaaaacgc tcacgccgat
    16621 taccgtgatt ttcggcgcga ttatgttcaa ccgtatgatg gaaaccacgg gctgcatcga
    16681 tgtcatccgc aaatggctgg cgaccatcag ccccaacccc gtagcgcaac tgatgattat
    16741 cggctgggct tttgccttta tgattgaagg cgcatccggc ttcggcacgc ctgccgcgat
    16801 tgccgcgccg attctgatga gcttgggctt caacccgttg aaagtggcga ttttcacttt
    16861 ggtgatgaac tccgtccccg tctctttcgg cgcggtcggt acgccgacct ggttcggttt
    16921 cgcaccgctg aacctgagtg ccgaagacat cctcgccatc ggcaggcaga ccggcgtaat
    16981 gcacttcttc gcaggtttcg tcatccccgt catcggcttg ggcttcatcg taccttggtc
    17041 tgaaatccgc aaaaacttgg gcttcgtcgc cattgccgtc ttctcctgca ccattcctta
    17101 tgtcgcattg gcgatggtca acgaagaatt cccgtcgctc gtcgccggcg cgatcggcct
    17161 gatggtgtcc gtattcgccg ccaaccaagg ctggggcttg agcaaagacc acgccaaaga
    17221 cccgaatgcc gaaaaagtgc cgttcgccca agtcgccaaa gcactcgccc ctttgggtat
    17281 gctgatcggc atgctggtgg ttacgcgcat caaacagctc ggtatcaaag gcattttgac
    17341 cagcaaagaa gaatggttca gcttccaact gccgtttgat ttgtccaaaa tcaccgtcag
    17401 cgactccctg acgattacct tcggcaatat tttcggacaa gatgtcagcg cgtcttacca
    17461 aacgctgtac gtcccggctt ggattccgtt tgtgctgacc gtttggattt gcatcctgct
    17521 gtataaaacc aaattcaaag atgcctggac gatttattcc gtaactttca atcaaaccaa
    17581 aaaaccgctg cttgccctga tgggcgcgct gattatggtt cagctgatgc tggtcggcgg
    17641 cgacaattcg atggtgaaaa tcatcggtaa ggaatttgcc gcaatggcgg gcgaacactg
    17701 ggtttatttc tcgccgtatc tgggcgcgat cggtgcgttc ttctccggct ccaacaccgt
    17761 gtccaacctg accttcggcc cgattcagca gcaaatcgcc ttggataccg gcctgtccgt
    17821 taccctgatt ctggcgttgc agtccgtcgg cggcgcgatg ggcaacatgg tgtgcctcaa
    17881 caacatcatc gccgtatgta ccgtattgga cgtgaaaaat tccgaaggtg cgattatcaa
    17941 gaaaaccgtt atcccgatgg cgatttacgg cgtgattgcc gtcgtcacgg caatgatttt
    18001 cttcctctag gcgaaacgcc gccatccata gcggcagcac gcggggaacg gaaaccgccg
    18061 ttccccgtca gggcagcgcg tcgggcagaa tctcggtgcc ggaacagccc gccccgccaa
    18121 acaaatgccg tctgaaaccg gaaaaggctt cagacggcat ttttccgccg tttgcattca
    18181 ggcgtaatga tgatgcggca tcttgccgtc aaggcggtag cgcgtaccgc aatacgggca
    18241 ggcaacgctg cccgattctc cttcgcccaa aggcaaaaac actctcggat gcccgttcca
    18301 ctgctcgttg tcgggccccg agcaatacag cggcagattt tccggcaaca cggaaatttc
    18361 ctgcggattc agattgtcca tttgattttc ctttgcgtgg ttggtgtacg cctattttac
    18421 gccatcggca ggctaaagga tattttcggc gcaaagccgc aatccgctat aatccccact
    18481 tttcagacgg cataccatga ctgcgcttac ccttccggaa gacatccgcc aacaagaacc
    18541 atccgttctg ctctataccc tcgtttccgc ctacctcgaa cacaccgccc aaactggcga
    18601 cgaatccctc tcctgcctgt ccgacgacca gcacacactg accgcattct gctacctcga
    18661 cagccaagtc gaagaaggcg gcttcgtgca actcatcgcc tccggctacg gcgaatacat
    18721 tttccgcaac ccgcttgccg acagcctgcg ccgctggaaa atcaaagccg tgccgaaagt
    18781 cttggacaaa gccaaagccc tctacgaaca acacggcgaa accatcgaaa cgctcgccga
    18841 cggaggcgca gacatcgaat ccctgcgcaa acagttcccc gactttgaag aatgggacgg
    18901 cgcatactac gaagccgccg aacaagattt acccctggtt gccgaacaca tacgttcaaa
    18961 ctgggaaacc ttcgcccata tcgggcaggc gtgattgcgt ctgtttccag cccgtgtaaa
    19021 acagcgtaaa atcggcaaac ccgaatgacc ttccgtcccc atcaaggagc aagctatgtt
    19081 cttcaagcac atcgaagccg cccccgccga tccgattctc ggtttgggcg aagcgttcaa
    19141 agccgaaacc cgccccgaaa aagtcaacct cggcatcggc gtgtacaaag acgcatccgg
    19201 cgcgacaccc attgtcaaag ccgtcaaaga ggctgaaaaa cgcctattgg aaagcgaaac
    19261 caccaaaaac tacctgacca tcgacggcgt tgccgactac aacgagcaaa cccaaatcct
    19321 gctgttcggc aaagaccacg aaatcatcgc cagccgccgc gccaaaaccg cgcaaagcct
    19381 cggcggtacg ggcgcattgc gtatcgcggc cgaatttgcc aaacgtcagt tggatgcgca
    19441 aaccatctgg atttccaatc cgacttggcc aaaccacaat gccatcgcca aagcggtcgg
    19501 catccaagac aaaccttacc gctactacga tgccgccaaa cacggtttgg attgggacgg
    19561 tatgattgaa gacttgaacc gggcgcaaaa aggcgacatc gtcctgctgc acggctgctg
    19621 ccacaaccct accggcatcg accctacgcc cgaacaatgg gaaactctgg caaaactttc
    19681 tgccgaaaaa ggctggctgc cgctgtttga ctttgcctac caaggcttcg gcaacggttt
    19741 ggaagaagat gcctacggct tgcgcgtgtt cttgaaacac aatacagaat tgctgattgc
    19801 cagctcttat tccaaaaact tcggtatgta caacgagcgc gtcggcgcgt tcactttggt
    19861 ggccgaagat gaagaaacag cagcccgcgc ccacagccaa gtcaaaacca tcatccgtac
    19921 cttgtattcc aacccggcct cacacggcgc gaataccatt gcgctggtgt tgaaaaatga
    19981 tgatttgaaa gcacaatgga ttgccgaact cgatgaaatg cgcggccgca tcaaagccat
    20041 gcgccaaaaa tttgttgcgt tgctcaaagc caaaggtgca agccaaaact ttgatttcat
    20101 tatcgaacaa aacggtatgt tctctttcag cggtctgact cccgaacaag ttgaccgttt
    20161 gaaaaacgag tttgccattt atgccgtccg ctccggccgc atcaacgttg ccggcattac
    20221 cgacgacaac atcgattatc tgtgcgaaag catcgtgaaa gtactgtaaa aagcacagcc
    20281 atatgaaaat gccgtctgaa ctttaaccag ttcagacggc atgtgttttt tgttaaaaaa
    20341 caataaacta taacaatttc aaacaagcta aaaacatcta taaaatcaac ccaatatgat
    20401 gaactcaaat aaaaaatacg aatatcatca ttctcaaatt caaaatttga acgccacagt
    20461 attatccgaa gccacaaaaa
//
LOCUS       DAXIEB010000025        20328 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000025 DAXIEB010000000
VERSION     DAXIEB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.025"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(69..2045)
                     /locus_tag="ACFOA3_000767"
     CDS             complement(69..2045)
                     /locus_tag="ACFOA3_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6897800.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFOA3_000768"
     CDS             2194..3033
                     /locus_tag="ACFOA3_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6897801.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFOA3_000769"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFOA3_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6897802.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTVNVSLVDLNFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4415..5140)
                     /gene="aat"
                     /locus_tag="ACFOA3_000770"
     CDS             complement(4415..5140)
                     /gene="aat"
                     /locus_tag="ACFOA3_000770"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6897803.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYQN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPCSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAMPLKEEIGRREVACRGL"
     gene            complement(5210..5644)
                     /gene="fur"
                     /locus_tag="ACFOA3_000771"
     CDS             complement(5210..5644)
                     /gene="fur"
                     /locus_tag="ACFOA3_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6897804.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5863..6240
                     /locus_tag="ACFOA3_000772"
     CDS             5863..6240
                     /locus_tag="ACFOA3_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6897805.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            6250..7059
                     /gene="dapB"
                     /locus_tag="ACFOA3_000773"
     CDS             6250..7059
                     /gene="dapB"
                     /locus_tag="ACFOA3_000773"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6897806.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIQAAGEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7308..7703)
                     /locus_tag="ACFOA3_000774"
     CDS             complement(7308..7703)
                     /locus_tag="ACFOA3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897807.1"
                     /translation="MPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFPQEIPT
                     PNYTDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGTLELIG
                     GRCDLLTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7721..7966)
                     /locus_tag="ACFOA3_000775"
     CDS             complement(7721..7966)
                     /locus_tag="ACFOA3_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897808.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEAELARMGLRL
                     KYNGLLKEEFKQLKFHLSNGGDLRSSNYKTIVFEKVK"
     gene            complement(7974..9146)
                     /gene="lpxB"
                     /locus_tag="ACFOA3_000776"
     CDS             complement(7974..9146)
                     /gene="lpxB"
                     /locus_tag="ACFOA3_000776"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6897809.1"
                     /translation="MWGQTNMTDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMESQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRKTARKTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9194..10186)
                     /locus_tag="ACFOA3_000777"
     CDS             complement(9194..10186)
                     /locus_tag="ACFOA3_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6897810.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDMVRIPPVRVAERKMPSERRAAVPARAFEAVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVRLPLFKYTGAQGEKMVRVSEDGQAAH
                     TVFRVLNRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            10680..13439
                     /locus_tag="ACFOA3_000778"
     CDS             10680..13439
                     /locus_tag="ACFOA3_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6897811.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTARQEARADTAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAYVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            complement(14105..15097)
                     /gene="pdxA"
                     /locus_tag="ACFOA3_000779"
     CDS             complement(14105..15097)
                     /gene="pdxA"
                     /locus_tag="ACFOA3_000779"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6897812.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPAAEAVESGQLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            15256..16683
                     /locus_tag="ACFOA3_000780"
     CDS             15256..16683
                     /locus_tag="ACFOA3_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6897813.1"
                     /translation="MNENFTEWLHGWINAINDQMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRVSKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            17008..18903
                     /gene="mnmG"
                     /locus_tag="ACFOA3_000781"
     CDS             17008..18903
                     /gene="mnmG"
                     /locus_tag="ACFOA3_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6897814.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGITPAAVALLMVHLK
                     RGFKDAK"
     gene            18972..19556
                     /gene="rnhB"
                     /locus_tag="ACFOA3_000782"
     CDS             18972..19556
                     /gene="rnhB"
                     /locus_tag="ACFOA3_000782"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6897815.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            19569..19814
                     /locus_tag="ACFOA3_000783"
     CDS             19569..19814
                     /locus_tag="ACFOA3_000783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897816.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(20132..>20328)
                     /locus_tag="ACFOA3_000784"
     CDS             complement(20132..>20328)
                     /locus_tag="ACFOA3_000784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897817.1"
                     /translation="IFVNPLYRHSRKNKKNKKIKNTKLKYRHSRAGGNLGLSVRKLIG
                     KNGFSNPETPDSCFRGNPVF"
BASE COUNT         5056 a         6367 c         4909 g         3996 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgagccctcc cctgatatgc cgtctgaagc
     3061 cgcttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat tcgccccgaa aatctaccca acccctcaac aaaggaacga accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 actatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcgtcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ccggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaaggcaaa ctcgacggac tcgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacaccac agtcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcgggtgcgc tcaaaggcgt tttgggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacgctga
     4141 ccaaagtcgt cgacggcaac atggtcaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatatcgat gttttcaagt
     4321 ttcagacggc atttttcacc gtgattttac ccggctgtcg tcatttccaa ttttaatccg
     4381 ccatatttga acctgaccga agcataagcc gccatcaaag ccccctgcac gccacttcgc
     4441 gccgcccgat ttcctctttc aacggcatgg cgttcaacat ccgcagacgt tcggcaaaat
     4501 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtgtcc tgctggcagt
     4561 ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
     4621 acgcatccgg ttgcaacgca aacattgatt cgccataaaa caccctgccg atttgcacgc
     4681 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaaag
     4741 aatgcgcgtg tcccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
     4801 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
     4861 agccgttgac tgcaacccga tagctgccgt tgcgtagcgt tttcgccagc gagcacggaa
     4921 cgtgcagacg ttcgggaaca atcaccgcac gcggcccgac cgcataccaa aaaaaccaac
     4981 cgtcccgcaa aaaccaagga aacacgccgt tctgatacgc ctcgagcagc cgccccgcgt
     5041 ccaaatccct gctcacgccg accagcccgt cgcaccgtgc caaagcatag gcaggatcgg
     5101 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
     5161 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
     5221 ccttggcctg acagtcgctg cacacgccgt acatatacag cgcgtgatcg acgatgcggt
     5281 agccgttttc ctcggcgatt ttgtcttgca gggcttcgat ttcgggattg tggaactccg
     5341 ttacctcgcc gcacttcacg cagacgatat ggtcgtggtg gtcgcccttg tccaactcat
     5401 aaaccgcctt gcccgtttca aaatggtggc gttgcaaaat gcccgcctgc tcaaattgcg
     5461 tcagcacgcg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     5521 acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc aaaatcttca
     5581 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     5641 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     5701 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     5761 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     5821 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     5881 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     5941 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     6001 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     6061 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     6121 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     6181 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     6241 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     6301 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tctccggcgc gctcgaacac
     6361 tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     6421 gccatttcag acgacgttga caccgttctc gcacaaagcg acgtactcat cgactttacc
     6481 cgccccgagc cgaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     6541 atcggcacaa caggcttcga cgatgcgggc aaagccgcca tccaagccgc aggcgaaaaa
     6601 acaggcgttg tcttcgccgc caacttcagc gtcggcgtca acctcacctt ccacctcctc
     6661 gacaccgtcg cccgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     6721 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggtgaagt catcgccggc
     6781 gcgctcggcc gcgacctcaa acaatgcgcc gtttacggcc gtgaaggcca caccggtccg
     6841 cgcgatccgt ccaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     6901 accgccctct tcgccaccga cggcgaacgc gtggaaatca cccacaaagc cagcagccgc
     6961 atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg taaacggcaa aacgggtttg
     7021 tacgatatgc aggatgtact cgggctgaac aaccgttaac ccccatacaa aataccgtct
     7081 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atcaatgttt
     7141 caacacacag gacgacacat aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt
     7201 acgcccctcc caaataaagt ctgattctac cgccctaaag ggcggggttt caaccgaaaa
     7261 ggaaatacga taaagttgcc tgcgcagcat aacaagttac ggctcgttta attgaaactc
     7321 tcctgatcta aaaattctaa ctctatttcc cggcaaacta tatctatact aaaaccagtt
     7381 agaagatcac atctaccgcc tatcaattcc agcgtacctc cataatccaa gatttcatca
     7441 tcctgcttta attcgccaag ctccaatata ttatcgatat ctttatcttg gctctttgta
     7501 gcgtatccat agataaattt cagatagctg aactgcttga atttaatttt tacccagtct
     7561 gtataatttg gtgtaggaat ctcttgagga aagtccggat aatccacatt ctggacgatg
     7621 atatggaatt catcgttttt caaagtatag tttaatagag tgccctcata tatatacaga
     7681 aattgataat ctttttttgg aatagtatta agttgtcata ttattttacc ttttcaaaaa
     7741 caatggtttt ataattactt gatctcaagt cgcctccatt gctcagatga aatttcagtt
     7801 gtttaaactc ttcctttaac agaccattat attttaacct taatcccatt cgggccagtt
     7861 ctgcttcatt tggaattcct cttaaatact tattgtttga tgtgccatta atcacaattt
     7921 ctcccccttc ttttgtaata cgtgctgctt caagaagata atccattaac cgctcaacat
     7981 cccgcctctt ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt caacagcagg
     8041 tgcaacgccc tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc gtaccagtcc
     8101 gccaacgccg ccgccagttt ttccggtacg gcatcatgtt gaagcagctc cggcacagcc
     8161 tccttaccca acaggatatt cggcaggccg acatgcggca ctttgatttt gcgtttcaca
     8221 taagcatagg tcagcggcga aatcttgtag ctgatgacca tcggacgctt gcacaacgcc
     8281 acctccaaag tcgccgtacc gctcgttacc agcaccgcat ccgccgccct gcacaccgtt
     8341 tcagactgtc tgtcgattac cgtcagcggc aatccggcaa actccggacg ctgcaaaact
     8401 tccgccaaac gccgcttcgt cgcctcagtt gcggcaggca gcaggaagcg tgcggcagga
     8461 tagcgtttca acaacaataa tgccgtctga aaaaacaccg gcgccatata atcgatttcg
     8521 ctgacgcggc tgccgggcag cagggcgaat acggggatgc cggcatccac gcccaaagtt
     8581 ttccgcgccg ttttacggtc gtcttccaag ggcataagct gcgccatcgg atgaccgaca
     8641 aactccgcac gtccgcccgc atcgagataa agctgcgact ccatcgggaa caggcacagc
     8701 acgcggttga cctgatgcac gattttgccc acgcgttccc gccgccacgc ccacaccgac
     8761 ggactgacat aatgcagggt cggaatgccc gcccgtttga gccgctccgc cacacccaga
     8821 ttaaaatcgg gcgcatcgat gccgacaaac acatcaggtt tcaacgacag caaatcccgt
     8881 accagccccc tgcgtatccg taaaatttcc ggcaggcgtt ttaccacctc ggcaaaaccg
     8941 cgcaccgcca gccgctcctg atcgtacagg ctctcgaaac cttccgcctt catcagttcg
     9001 ccgccgatac cgacaaaacg cgcctgcggg caacgctcgc ggatggcgcg tatcaggtgc
     9061 gcccccaaca ggtcgcccga cgcttcgccg acactgacgg caatcaaagg gcttttttta
     9121 tcagtcatat tcgtctgtcc ccacatactt tcacgtcccg atgccgtctg aaggcttcag
     9181 acggcatcgg ggttcacatt ttcgtcccgt tttccaacat caccgcaaac tgtgccaagt
     9241 ccagcggcag ctccgccttc aacaccagcg gctcgcccgt gagcggatgg ttcaagtgca
     9301 gctcggacgc gtgcaaaaac atccgcttca aacccaactt ctgcaaacgc cggttcgcct
     9361 gataatcacc gtagcgttcg tcgcccgcaa tcggacagcc ttgggattgc aggtggacgc
     9421 ggatttggtg cgtgcgcccc gttttcagcg ttgcccgcac caaagtcagg tgcgacagcc
     9481 cgacaccgtg caaaatgccg tctgaaaacc gatttaacac acggaacacc gtatgcgcag
     9541 cctgcccgtc ctcgctaacg cgtaccatct tttcgccttg tgcgcccgtg tatttgaaca
     9601 gcggcagcct gacgtggaaa ttatcatccg gcagcttgcc cacgcccaat gcgagataga
     9661 tttttttggg gtggtcgttg cggatggctt cgtgcagttt gacgagcgcg ctgcgtttct
     9721 tcgccaccat cagcaagccg ctcgtatcct tgtccaaacg gtggacgagt tcaagataac
     9781 gcgcctccgg acgggcgcgg cgcaactgtt cgataacgcc gaaactcacg ccgctgccgc
     9841 cgtgcaccgc cacgccggaa ggcttattga ccaccagcag cgcgtcgtcc tcatacaccg
     9901 cctcaaacgc acgcgccggt acggcggcac gcctttcaga cggcatcttg cgctccgcca
     9961 cgcgcacagg cggaatccgc accatatccc cctccgcaat acggctgtcg ggtttgcagc
    10021 gtttcttgtt caaccgcacc tcgccggcgc ggataatgcg gtggatatgg ctcttgggca
    10081 cgcccttgag gatttttatc agatagttat caaggcgttg ccccgcctcg tgttcggcaa
    10141 cccctatcag gctgaccgaa tctttgctta tttcgtgcgt tttcatctat aatccgaaca
    10201 tccgtttcag caaaaagcgc gcccgccgcg cccccctgaa acggtttact ttaaaacgga
    10261 attttatatt aaaaacgcac tccgcaaagc attttccctg cgcctgttcc cagccggcag
    10321 gcgcagaacg tagtcaagtt tgaattgatt ttgccgtttc ggcgcggaag taagacgaca
    10381 gccgggccca agccccaaac gcaaaccgcc cgaaaacaca ggcataacga taacaagaag
    10441 aagccggccc ccatttttcc gaccataccg ctcggaacgc ggcagacaga ccgccgcatc
    10501 gatctttatt gccttcttta tccgaccctt ccgcacacgg ttcctcggaa cgtgcatccc
    10561 tgcggatttt caactcaaaa actgattacc ggttttatcc gaagacatcg gaaaacccag
    10621 ccttacggaa tgccgaaccg gaccggcgcg cccgaatgcc gcccgtccac gaggtgatca
    10681 tgaaaagaat gttattcaac gcaacgcagg ccgaagagct gcgcgttgcc atcgtcgacg
    10741 gacaaaacct gctggatttg gatatcgaaa cgctgggcaa agaacagcgc aaaggcaata
    10801 tctacaaagg catcattacc cgcatcgagc cgtcgctgga agcgtgtttc gtcgattacg
    10861 gaaccgaccg ccacggcttc ttgccgttta aagaagtctc ccgctcatac ttccaagact
    10921 acgaaggcgg acgcgcgcgc atccaagacg tgctcaaaga aggcatggaa gtcatcgtcc
    10981 aagtcgaaaa agacgagcgc ggcaacaaag gcgcggcact gaccaccttc atcagcctcg
    11041 ccggacgcta tctggtattg atgccgaaca acccgcgcgg cggcggcgta tcccgccgta
    11101 tcgaaggcga agagcgtcaa gagttgaaag ccgccatggc ggaactcgac attccgaacg
    11161 gcatgagcat catcgcccgt accgccggca tcggccgcag tgcggaagag ttggaatggg
    11221 acttgaacta cctcaaacaa ctctggcagg cgattgaaga agcaggaaaa gcgcatcacg
    11281 acccttacct gctctttatg gaaagctcgc tgctgatccg agccatccgc gactatttcc
    11341 gccccgacat cggcgagatt ttggtggaca atcaagaagt ttacgaccaa gttgccgagt
    11401 tcatgagcta cgtcatgccg ggcaatatag gccgtctgaa actctacgaa gaccacacgc
    11461 cgctgttttc ccgcttccaa atcgaacatc aaatcgaaag cgcgttttcg cgcagcgtca
    11521 gcctgccttc cggcggcgcg attgtgatcg accacaccga agccctcgtc tccatcgacg
    11581 tgaactccgc acgcgccaca cgcggcgcgg acatcgaaga caccgcgttc aaaaccaata
    11641 tggaagccgc cgaagaagtc gcccgacaaa tgcgcctgcg cgacttgggc ggtttggtcg
    11701 tcatcgactt catcgatatg gaaaacccca aacaccagcg cgatgtggaa aacgtcctgc
    11761 gcgacgcgct caaaaaagac cgcgcccgcg tgcagatggg caaactgtcc cgtttcggac
    11821 ttttagaatt gagccgccaa cgtttgaaac cggctttggg cgaaagcagc catgttgcgt
    11881 gtccgcgctg tgccggcacc ggcgtgattc ggggcatcga atccaccgcc ctgcacgttt
    11941 tgcgcatcat tcaagaagaa gcaatgaaag acaacaccgg cgaagtgcgc gcacaagtgc
    12001 ccgtcgatgt cgccaccttc ctgctgaacg aaaaacgcgc cgagctgttt gcgatggaag
    12061 agcgtttgga tgtcaacgtc gtcctgattc cgaacatcca cctcgaaaat ccgcactacg
    12121 aaatcaaccg catccgcacc gacgacgtag aagaagacgg cgaaccgagc tacaagcgcg
    12181 ttgccgagcc agaagaagac gaatccgcca aaccgttcgg cggcgaaaaa gccaaagccg
    12241 cccgtcccga acccgccgtc aaaggcgtgc gccacaccag ccccgccccg actgccgccc
    12301 ccgagaaaaa aacctcctgg tgggacagct tcaaagcttg gctcaaacgc attttcggcg
    12361 gcagcgaaac ccaagccgca cccgctgccg aaacctccga aaaacgcagc acggcaaacc
    12421 gcagcggcag ccgcgccaac aaccgccgcc aaaacccgcg ccgcagcaaa cgcgaaggca
    12481 gcaaagtaga agtccgcgaa gtggcaggca aaactgccag acaggaagcg cgtgccgaca
    12541 cagccgaaac gcgcaacaac ggcaaccgcc gccgcaacga acgcggcgac cgtgccgccg
    12601 aacgtgccaa cgaagcggaa atccaaagcc gcaacgtaca gcctgccgca accgttgcag
    12661 atgccgcacc gtccgaaacc gaagtgcaaa ccggaaaacg ccgccgcaac ggcagccgca
    12721 acgaacgcgg ccaaaccgcg ccggaaaccg ccgccgttgc cgaaacagcc gttcagacag
    12781 cggaaaacac gccgtccgaa ccgcataccg cagaagacaa aggcagcaag cccaaatccg
    12841 aacgcaaccg ccgcgaacgc gacagccgcg acgccaaaga acgccgcgag cgcaacaatc
    12901 agcgcgaccg ccgtcaaaac ggcaaaaaac gcaatattcc gtctgccgcc aaaatcgagc
    12961 agtacctgaa tattcacgac accgccgaca aagtccgttc cgccgccgcg tacgttttcg
    13021 gcgaaaccga cgcaaacgcg ccgattaccg tcagcattgc cgatccggtt gcagaaaggg
    13081 atcttccgac agcatctccc gccgtttcaa acggcgacgc accggtttat gatgcggcgg
    13141 aaaaaatccg ccgtgccacc gccgccatcc tgcccgaagg cgcgacaccg aaagccgaag
    13201 cacaggaaat gccgtctgaa accgcaacct ttacggctgc ggcggaacag gcacgggaaa
    13261 ccgcacaaac cggcggactc gtcctgatcg aaaccgaccc tgccgcattg aaggcatggg
    13321 cggcacaacc cgaagtccaa accggacgcg gtttgcgccg ttccgaacag cccaagccgt
    13381 ctgaagtcgc aaccgtccct gccgaagaaa tgatccaagt cgaaacccgg caaggctgaa
    13441 ccgacggcgg caaaaagagg ttctgttccg cagaacctct tttttacata gattcggata
    13501 cctgcaatgc cgtctgaaac ttcgccattc ccgtcattcc cgccaccttt cgtcattccc
    13561 gtgaaaacgg gaatctagaa cctcaaactt tcggataatc tttgaatatt gccgccgccc
    13621 aaaggtctgg attcccacaa cattccgtca ttcccgtgaa aacgggaatc tagaacctct
    13681 aaactttcag ataatctttg aatattgccg tcgcccaaag gcctggattc ccgcctgcgc
    13741 gggaatgacg gtttagaagt tgcccgaaac ctcaaaaaaa aaccgaaacc gaacaagccg
    13801 gattcccgca acattccgtc attcccgtga aaacgggaat ctagaacctc taaactttca
    13861 gataatcttt gaatattgcc gctgtccaat ggtctggatt cccacaacct ttcgtcattc
    13921 ccgtgaaaac gggaatctag aacctcaaac tttcggataa tctttgaata ttgccgctgt
    13981 ccaatggtct ggattcccgc ctgcgcggga atgacgattt ggaaattacc cgaaacccaa
    14041 aaacaactga aaccgaacag accggattcc cgcctgcgcg gaaatgacgg gtcttttatc
    14101 atctttaaag gctgccgcgc gccatctcga cggcggtctc cacggcagtt atcaggctgc
    14161 cggaatccgc cctgccggtt gccgccaaat caagcgcggt gccgtgatcg acggaggtgc
    14221 ggataaaggg cagacccagc gtgatgttca cgccctgtcc gaagctgtgg tatttcaaca
    14281 cgggcagccc ttggtcgtgg tacatcgcca atacggcatc cgcaccttcg agcataaacg
    14341 gttggaacaa ggtatccgcc ggatacgggc cggcgaggtt tatcccttcg cggcgcaggt
    14401 tttcaagcgc ggggataatg gtgtcggttt cttcgtgtcc gaggtgtccg ccctcgccgg
    14461 cgtgggggtt gagtccggcg actaggattt tgggattttt gatgccgaat ttgtgtttta
    14521 agtcgtgatg cagaatgcgt gcgacgcttt caatcagcgg ttgcgtgatg gcggcggcaa
    14581 cgtctttcag cggcaggtgg gtcgttacga gggcgacgcg caggcctttg ccggcaagca
    14641 tcatcacaac ctgccccgtg ccgctttttt ccgccagata ttcggtgtgt ccgctgaaaa
    14701 aacctgtgct tgcgcgcgcg tcgttgatga tgcctttgtg cagcggcgcg gtaacgatgc
    14761 cgtcgaaaat gccgtctgaa atgcctgcga gcgcggtgtc caaaagttgc agcacatagg
    14821 cggcgttggc gggattaagc tgcccggact caaccgcttc ggcggcaggg atgtgcagca
    14881 cttccagctc gccttctccg ggtgcgccgc ctgattccgg atcgaagtcg cgcaggacga
    14941 cgcttttgcc caaggcttcg gcgcgcgcgc gcaacaggtg tttgtcgccc aataccgcgc
    15001 agcggcaggg caggcgtgca aacgccaagt ccaaacaaat atcggggccg atgccggcag
    15061 gctcgccgga agtaacggca aaaacaggct gtttcatcgt gtttgctcca aacaaaatgc
    15121 gattctaacg ccgcagccgc gcggcgatgt aaatttttct gattttgttg acaatctgct
    15181 agaatagaag tttacaaaat ttaaaccctg cttacatacc gccatatgtg cgggtttcaa
    15241 ctttaaggaa gcgatatgaa cgagaacttt accgaatggc tgcacggctg gataaacgcc
    15301 atcaacgacc aaatgtggtc atacttggtt tatatgcttt tgggtacggg gcttttcttc
    15361 accgtaacca cgggctttgt ccaattccgc ctgttcgggc gcagcatcaa agaaatgctc
    15421 ggcggccgca aacaggggga cgaccctcac ggcatcacgc cgtttcaggc atttgtaacc
    15481 ggccttgcca gccgcgtggg cgtgggcaat atcgcgggcg tggcaatcgc catcaaagtc
    15541 ggcgggccgg gcgcggtgtt ctggatgtgg gtaaccgcct taatcggtat gagttcggcg
    15601 tttgtcgaat cttcgctggc gcagctcttt aaagtccgcg actacgacaa ccaccacttc
    15661 cgtggcggcc ctgcctacta catcactcaa gggctggggc agaaatggct gggcgtgttg
    15721 ttcgccctga gcctgatttt ctgtttcggc tttgtgtttg aagccgtaca gaccaatacc
    15781 atcgccgata ccgtcaaagc ggcgtggggc tgggagcctc attatgtcgg cgttgccctg
    15841 gtgattttaa ccgcgccgat tatcttcggc ggcatcaggc gcgtatctaa agcggcggaa
    15901 atcgtcgtcc ccctgatggc ggttttgtac ctctttatcg cgcttttcat cattttgacc
    15961 aatattccga tgattccgga cgtgttcggt cagatttttt cgggcgcgtt caaattcgac
    16021 gcggcagcag gcggcttact cggcggtctg atttcgcaaa cgatgatgat gggcatcaaa
    16081 cgcggcctgt attccaacga ggcgggtatg ggttccgcgc cgaacgccgc cgccgccgcc
    16141 gaagtgaaac accctgtttc acaaggtatg attcaaatgc tgggcgtgtt tgtcgatacc
    16201 atcatcgttt gttcttgcac cgccttcatc atcttgattt accaacagcc ttacggcgat
    16261 ttgagcggtg cggcgctgac gcaggcggcg attgtcagcc aagtggggca atggggcgcg
    16321 ggcttcctcg ccgtcatcct gtttatgttt gccttttcca ccgttatcgg caactatgcc
    16381 tatgccgagt ccaacgtcca attcatcaaa agccattggc tgattaccgc cgtcttccgt
    16441 atgctggttt tggcgtgggt ctatttcggc gcggttgcca atgtgccttt ggtctgggat
    16501 atggcggata tggcgatggg cattatggcg tggatcaacc ttgtcgccat cctgctgctc
    16561 tcgccactgg cgtttatgct gctgcgcgat tacaccgcca agctgaaaat gggcaaagac
    16621 cccgagttca aactttccga acatccgggc ctgaaacgcc gtatcaaatc cgacgtttgg
    16681 taaatcccgc ccttaccgga gccgcttccc cgcgaagcgg cttttccctt tccgcacact
    16741 gtaaaaacag ggcgaacaag cgtacaatcc caacccttta cttttgaatc catttcgttt
    16801 ttcagacggc atattgaata tagtggttta acaaaaatca ggacaaggcg acgaagccgc
    16861 agacagtaca agcagtacga aaccgattca cttggtgctt cagcacctta gagaatcgtt
    16921 ctctttgagc taaggcgagg caacgctgta ctggtttttg ttaatccact ataaatgccg
    16981 tctgaaacac cgtcaggcaa tacacacatg acccacatga tttaccccaa aacctacgac
    17041 gttatcgtcg tcggcggcgg acacgccggc acagaagccg cgctcgccgc cgcccgtatg
    17101 ggcgcgcaga cgcttttgct cacacacaat attgaaacgc tcggacaaat gtcgtgcaac
    17161 ccttccatcg gcggcatcgg caaagggcat ttggtgcgcg aactcgacgc gctcggcggc
    17221 gcgatggcgt tggcaaccga caaatccggc atccagttcc gccgtctgaa cgccagcaaa
    17281 ggcgcggcag tacgggccac gcgcgcgcag gcggatcgta tcctatataa agccgccatc
    17341 cgcgaaatgt tggaaaacca agaaaacctc gaccttttcc aacaagccgt cgaagacgta
    17401 acgctcgatg gcgaccgcat ttcaggcgta atcaccgcga tgggcgtgga gtttaaagca
    17461 cgcgccgtgg tgttgaccgc aggcacgttt ttgtccggca aaatccacat cggtttggaa
    17521 aactacgagg gcggacgcgc cggcgacccc gccgccaaat cgctgggcgg acgtttgcgc
    17581 gaattgaacc tgccgcaagg ccgtctgaaa accggcacgc cgccgcgcat cgacggacgc
    17641 acgattgact tctcccaact gaccgaacag cccggcgaca cgcccgttcc cgtcatgtcc
    17701 gtacgcggca acgccgatat gcacccgcgc caagtatcct gctggattac gcataccaac
    17761 acgcaaaccc acgacatcat ccgctcaggc ttcgaccgca gccccatgtt caccggcaaa
    17821 atcgaaggcg tcggtccgcg ctactgcccg tctatcgaag acaaaatcaa ccgcttcgcc
    17881 gacaaagaca gccaccagat tttcctcgaa cccgaaggcc tgactacgta cgaatactat
    17941 cctaacggta tctccaccag cctgccgttc gacatccaaa tcgcgcttgt acgcagcatg
    18001 aaaggtttgg aaaacgccca tatcctgcgc cccggctacg ccatcgaata cgactacttc
    18061 gatccgcgca acctcaaagc cagcctcgaa accaaaacca ttgagggact gtttttcgcc
    18121 gggcaaatca acggcacgac cggctacgaa gaagccgccg cgcaaggttt attggcaggc
    18181 gcgaacgccg tgcaatatgt gcgcgaacaa gacccgctcc tgctgcgccg cgaacaagcc
    18241 tacctcggcg tattggtgga cgacctcatc accaaaggcg tgaacgaacc ttaccgcatg
    18301 ttcaccagcc gcgccgaata ccgcctgcaa ctcagggaag acaacgccga tatgcgcctg
    18361 accgaagacg gctacaaaat cggcttggtg ggtgaagcgc aatggcgtat gttcaacgaa
    18421 aaacgcgaag ccatcgaacg cgaaatccaa cgtttgaaaa caacgtggta cacgccgcaa
    18481 aaactcgctg aagacgaaca aatccgcgtg ttcggacaaa aactcagccg cgaagccaac
    18541 ctgcacgacc tcctgcgccg cccaaacctc gactacgccg cgctgatgac gctcgaaggc
    18601 gcgatgccgt ctgaaaacct ctccgccgaa gtcatcgagc aagtcgaaat ccaagtcaaa
    18661 taccaaggct atatcgaccg ccaaaacgaa gaaatcgaca gccgccgcga catcgaaacc
    18721 ttaaaactgc ccgacggcat cgattacggc aaagtcaaag gcttgtcggc agaagtgcag
    18781 caaaagctca atcagcacaa acccgaaacc gtcggacaag ccagccgcat ttccggcata
    18841 acccctgcgg cagtggcatt gctgatggtg catttgaagc gcgggtttaa agacgcgaaa
    18901 taaacacatc ggtacgatgc cgtctgaaac ccttttcaga cggcattcct actatcccga
    18961 caggaaacat catgcacata ctgaccgccg gcgtggacga ggcaggacgc gggcctttag
    19021 tcggcagcgt gtttgccgcc gccgtcatcc ttccggaaac attcgacctg cccggactga
    19081 ccgactccaa aaaactcagc gagaaaaaac gcgacgcgct tgccgaaatg ataaaaaatc
    19141 aggcggccgc gtggcacgtc gccgccgcca cgcccgaaga aatcgccagc ctcaacatcc
    19201 tgcacgctac catgctcgcc atgaaacgcg ccgttgacgg tttggctgtg cgtcccgaaa
    19261 gaatattcat cgacggcaac cgcattccag aacatttagg tatccctgcc gaagccgtcg
    19321 tcaaaggcga cagcaaaatc atcgaaatct ccgccgcatc cgttttggca aagaccgcac
    19381 gcgatgcgga aatgtacgca ctggcgcaac gccatcccca atacggtttc gacaaacaca
    19441 aaggttacgg cacgaagcag catctggaag ccctcaaaca atacggcgtg ctgcccgaac
    19501 atcgccgcga cttcgccccc gtcagaaacc tgctcgcgca gcagacgctg ttttaaaccg
    19561 gcacaaaaat gccgtctgaa cttcagacgg catttccagc atcgcaactt caaagagaaa
    19621 gaacccatac cgccattccc gcgaaaaaca gaaaatcaaa aaagaaacct aaaatccgtc
    19681 attcccgcgc aggcgggaat ccaatccgtc cggtttccgt tttttttgaa tttcaggtaa
    19741 cttccaaacc gccattcccg caaaagcggg aatctagaaa ctcaaagctg caagaattta
    19801 tcaaaaatga ctgaagctca aaaaaccgga ttcctacgaa aacaggaatc cggagtctca
    19861 gggctggcaa aaccgtttta cccgataagt ttccgtaccg acagacctag attcccgcct
    19921 tcgcgggaat gacgaaattt taggcttctg ttttgatttt ttgtttttgc gggaatgacg
    19981 aaattttaga ttgcaggcat ttatcggata aaacagaaat taagcgtgac gaaaatttat
    20041 ccgaaatcac agcaactttt ccgcgtcatt cccgcaaaag cgggaatcta gaaactcaaa
    20101 gctgcaagaa tttatcaaaa atgactgaaa ctcaaaaaac cggattcccg cgaaaacagg
    20161 aatccggagt ctcagggttg gaaaaaccgt ttttcccgat aagtttccgt accgacagac
    20221 ctagattccc gcctgcacgg gaatgacgat atttcagttt tgtgtttttg atttttttgt
    20281 tttttttgtt tttgcgggaa tgacgatata gtggattaac aaaaatca
//
LOCUS       DAXIEB010000026        20194 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000026 DAXIEB010000000
VERSION     DAXIEB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20194
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.026"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(153..3005)
                     /gene="gcvP"
                     /locus_tag="ACFOA3_000785"
     CDS             complement(153..3005)
                     /gene="gcvP"
                     /locus_tag="ACFOA3_000785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6897818.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSGRFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVAADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDTFTFADDVK
                     GRLNAGLLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     AVAAAAYGSASILPITWMYLTMIGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPQ
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3036..3398)
                     /locus_tag="ACFOA3_000786"
     CDS             complement(3036..3398)
                     /locus_tag="ACFOA3_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897819.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRASLMAMLPL
                     FVVILVLLNIVFPLLAHPLAWLVPAGFMVLVGGFPLSLPLVALLVLTCCILLARCPPL
                     SRLLCYPCPNHPMSKNSA"
     gene            complement(3569..4210)
                     /locus_tag="ACFOA3_000787"
     CDS             complement(3569..4210)
                     /locus_tag="ACFOA3_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6897820.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPDESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSKEYGIPLFGGAWAEILSDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4200..4751)
                     /locus_tag="ACFOA3_000788"
     CDS             complement(4200..4751)
                     /locus_tag="ACFOA3_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6897821.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWMLMLKKRQAFQTAFKGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            complement(4755..5273)
                     /locus_tag="ACFOA3_000789"
     CDS             complement(4755..5273)
                     /locus_tag="ACFOA3_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6897822.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5273..6934)
                     /gene="pqiB"
                     /locus_tag="ACFOA3_000790"
     CDS             complement(5273..6934)
                     /gene="pqiB"
                     /locus_tag="ACFOA3_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6897823.1"
                     /translation="MTDNSPPPNRHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINANEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSTSPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFGKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRITLQGISPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(6927..8387)
                     /locus_tag="ACFOA3_000791"
     CDS             complement(6927..8387)
                     /locus_tag="ACFOA3_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC6897824.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRFFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDSGEAAFCPS
                     CGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEDKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMAVLIA
                     AARFALPVGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8562..9191
                     /locus_tag="ACFOA3_000792"
     CDS             8562..9191
                     /locus_tag="ACFOA3_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6897825.1"
                     /translation="MSETENQALTFAKRLKADTTTVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9324..10649
                     /locus_tag="ACFOA3_000793"
     CDS             9324..10649
                     /locus_tag="ACFOA3_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6897826.1"
                     /translation="MTIPFKPSLLAMASVLALSACISGGGDAPAVKSSETPSNKVAPP
                     TDTASPATGADTASPSAGGDGGDSKTEIPPKDTQGEGAPAGTDGGVQNDASQHNDNAP
                     ATGTPETQPENNRMPGSDAVGSDGGNQDTDAPKNTESQDGTEENAVSEGTGMPVSPPS
                     ENAENTEVAMNAEDANPPSKAENSDEVGQGGMQADDGRQDAKEGLQGDDPAAHSPQDQ
                     DQQPQNAADTQPKTGVFGEKYFDGTGEITTLVIGGETIDLTKADEPVSSRNHPGLMSD
                     SDFEKVMVSQGQYQHLIYGAAKLQDGTYTLFVQGKPTENLPTGMMAKYQGKVLHFVGK
                     YKDNWFNNSAYRAAGTFTADVDFSRKTLTGKIDTKDPSGMGMQEFTANIDGNGFQGKW
                     KSNNDQGDISGKFYGQDGAEMAGRYSYKDNNEHKSGFGVFAGKKEAKAR"
     gene            complement(10839..10949)
                     /locus_tag="ACFOA3_000794"
                     /pseudo
     CDS             complement(10839..10949)
                     /locus_tag="ACFOA3_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            11019..11306
                     /locus_tag="ACFOA3_000795"
     CDS             11019..11306
                     /locus_tag="ACFOA3_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897827.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVDGLGGDESKDMPECRK"
     gene            11294..11635
                     /locus_tag="ACFOA3_000796"
     CDS             11294..11635
                     /locus_tag="ACFOA3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6897828.1"
                     /translation="MPQVKLIITRPAQSDLVRIYNFLNDLGAEHQANTVMRLLKNSFV
                     STQGSPSNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFR
                     ENAYRLSFPFL"
     gene            complement(11887..12363)
                     /locus_tag="ACFOA3_000797"
     CDS             complement(11887..12363)
                     /locus_tag="ACFOA3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6897829.1"
                     /translation="MLAACQSGRAPKNAHTASAGTPAGESCKVIRGDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACERA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(12496..13851)
                     /gene="yegQ"
                     /locus_tag="ACFOA3_000798"
     CDS             complement(12496..13851)
                     /gene="yegQ"
                     /locus_tag="ACFOA3_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6897830.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            14176..14964
                     /locus_tag="ACFOA3_000799"
     CDS             14176..14964
                     /locus_tag="ACFOA3_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6897831.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKYIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGWGMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQELGVI"
     gene            complement(15012..15611)
                     /locus_tag="ACFOA3_000800"
     CDS             complement(15012..15611)
                     /locus_tag="ACFOA3_000800"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6897832.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            15739..16356
                     /gene="gmk"
                     /locus_tag="ACFOA3_000801"
     CDS             15739..16356
                     /gene="gmk"
                     /locus_tag="ACFOA3_000801"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6897833.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSIEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDYVVVNDDLARAEEDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            16415..16621
                     /gene="rpoZ"
                     /locus_tag="ACFOA3_000802"
     CDS             16415..16621
                     /gene="rpoZ"
                     /locus_tag="ACFOA3_000802"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6897834.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLARNK"
     gene            16710..18866
                     /locus_tag="ACFOA3_000803"
     CDS             16710..18866
                     /locus_tag="ACFOA3_000803"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6897835.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVVVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIAGHWSYKSDSQTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRYDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLKDLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGKHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(18933..20117)
                     /gene="coaBC"
                     /locus_tag="ACFOA3_000804"
     CDS             complement(18933..20117)
                     /gene="coaBC"
                     /locus_tag="ACFOA3_000804"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6897836.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGSGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
BASE COUNT         4770 a         4937 c         5614 g         4873 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtttgcacg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaaccca tttgtcttcg gggtttcaac ggtcagtctt cataattttc catcggtggg
      181 cagctgcaca cgaggttgcg gtcgccgtac acgtcgtcca cgcggttgac gaagggccaa
      241 aacttgtgtt cgcggacgaa gggctgcggg aagacggctt cttcgcggga atacggatgc
      301 gcccattcgc cggttatatc tgcggcggtg tgcggcgcgt tgaccagtgg gttgtcgtct
      361 ttcggccatt ctccgtcgat gactttctgc acttcgcggc gaatggattt cagggcggcg
      421 ataaagcggt cgagttcggc tttgctttcg ctctcggtcg gctcgatcat cagcgtaccg
      481 gcaacgggga aggaaaccgt cggcgcgtgg aagccgtagt ccatcaggcg tttggcgatg
      541 tcggtttcgg taatgccact ttcggctttg agcggacgca aatcaacgat acattcgtgc
      601 gcgacgcggc cgtttttgcc tgtatacaga atcggatagt cttcgctcaa gcgtttagcg
      661 acgtagttgg cgttgagcaa tgcccagcgt gttgcctgct ccatgccttg tttgcctatc
      721 atggtcaggt acatccaagt aatcggcagg atggatgcag aaccatatgc tgcggcggca
      781 acggcggttt gcccggcact tgcgctgtgt gtgtcggtca gggtatgccc cggtgcgaac
      841 ggggcgaggt gggctttcaa gccaatcgga cccatgcccg ggccgccgcc gccgtgaggg
      901 atacagaagg ttttgtgcag gttcatgtgc aacacgtccg cgccgacttc ggcaggctgc
      961 atgatgccga tttgggcgtt gaggttggca ccgtccatat aaacctgtcc gccgttttca
     1021 tggatgatgc ggcagatgtc gcggatgcct tcttcgtaca cgccgtgggt agacggatag
     1081 gtaatcatga tggcggacaa agtgtcgcgg tgttgctcgg ctttggcttt caaatcgtca
     1141 atattgacgt tgccgtgttc gtcggtatcg acaacgacga ctttcaagcc cagcatggcc
     1201 gcagtcgcag ggttggtacc gtgggcggat ttgggaatca ggcagatgtt gcggtgtccc
     1261 tcgccgttgg cttcttggta acggcggatg gacagcatgc cgctgtattc gccctgcgcg
     1321 cctgagttgg gctggaagga aatcgcgtca aaaccggtga tggctttcag gctgttttcc
     1381 atatcggcga gcaattcgcg gtagccggcg gtttgcgcct cgggggcgta ggggtggatg
     1441 tcggagaact ccgcccaagt aatcggcagc atttccgcag tcgcgttgag tttcatggtg
     1501 cagctgccca atgaaatcat gctgcggttc atcgccaagt cgcggtcttc gagttttttc
     1561 agatagcgca gcatttcgtg ttcggtgtgg taacggttga acacgggatg ttgcagaatg
     1621 tcgtcctgac gcagcaatcc ggcgttcaga cggcctttga catcatcggc aaatgtgaac
     1681 gtatccttgc cggtaaacgc gcggtacaaa tcaaccaaat cttcgtatgc cgatgtttca
     1741 tggaatgcag ccgcaacttg agtatcgttg acgcggcgca ggttgtaacc cgactctaaa
     1801 gcagcggcaa acacttggtc tgctttctct ttgttgccga aatcgacggt aacggtatcg
     1861 aagaagactt tgtgaaccac attcaggccg tctgaaacca gcgcgtcggc aaaagcagaa
     1921 gccagcgcgt gaatgcggtt ggcaatgcgt ttcacacctt cagggccgtg gtaaacagcg
     1981 tacataccgg ccaagttcgc cagcaatgcc tgcgcggtgc aaatattgga tgtcgctttt
     2041 tcgcggcgga tgtgttgttc gcgggtggac aaagccatgc gcagcgcagg tttgcccgat
     2101 gcgtctttgg atacgccgat gatgcggccc ggggcggaac gcttgaactc gtccttaaac
     2161 gcgaaataag cggcgtgcgg accgccgaag cccatcggta cgccgaagcg ttgcgtgttg
     2221 cccaaagcaa tatctgcgcc caattcggca ggcggtttca gcaaaaccaa gctcatgatg
     2281 tcggcggcaa cggcgacaat cgtgcctttg gctttcagac ggcctatgac gctttgcaag
     2341 tcttgcacgt cgccgtcttt gccgacatat tggaacagcg cgccgaagta ttcgccttca
     2401 tccgctttgg caaaatcgcc gactaccagc tcgaagccga aatatttggc gcgggttttc
     2461 atcacgtcca aagtttgcgg atacacgcgc gcatccacaa agaaacgccc ggatttcact
     2521 ttacccacgc ggtgcgccat cgccatcgct tcggcggcgg cggtcgcttc gtccagcaaa
     2581 gacgcgcctg ccacgggaaa accggtcaaa tcgatacaca cttgttggaa gttcaacaac
     2641 gcttccaaac gaccctgagc aatttccgcc tgatacgggg tgtaggcggt ataccaaccc
     2701 ggattttcca atacgttacg caaaatcaca ttcggcacac gggtcgggta atagcccaag
     2761 ccgatatagg acttgttgat tacgtttttc gacgcaatgc ctttcaattt cgccaatgcg
     2821 tctgcctcgg tcaaggcctc gggcaaatcg agttcagacg gcatacgaat gctttgcggc
     2881 acggtattgc cgacaaaatc gtccatgctt ttctcgccga cagcggcaag caacgccgct
     2941 tcgtcgccaa agctcaaatg gcgcgcggca aattcgttcg ggttgaacaa ttcagatagt
     3001 ttcatttcta ctccttcggg tttgtagggc tgccatcagg cagaattttt agacatcgga
     3061 tgattcgggc aagggtagca caaaagacgg gataatggcg gacaacgcgc cagcagaatg
     3121 cagcaggtca ggacaagcag cgccacaagc ggcagcgata agggaaagcc gccgaccaaa
     3181 accatgaaac ctgcaggcac cagccaagcc aagggatgcg ccagaagcgg aaaaacaata
     3241 ttgagcagaa ccaaaatcac gacaaacagc ggcagcatcg ccatcaggct ggcacgcctg
     3301 tatgttgcgt acgggtcgga tgcttcttca ggttcgcaat attccataat ttcagcctgt
     3361 ccgggcttta tccacagcag gttcggacgg tcggtcatgc cgtctgaacc ggcaggccgt
     3421 cttttttaaa aaacgccata ccgctgcaac ttcatgcggc tgtatggcgt aggttttgca
     3481 cttcagacgg catatgcgtc ccgaagcagt ttggtgtccc agtcggttca tgattatata
     3541 aacaacttgg gtgcggataa acctttgttt atctaaaccc ttgttttctc aaaaattgat
     3601 tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc gtggatttgg tcggatttca
     3661 gattattatc gctcaaaatt tccgcccacg cgccgccgaa cagcggaatg ccatattcct
     3721 tggacaaatc ctcatacagc ggatggtcgc tcaaatgccc gaacaatgcg cccagtgtga
     3781 tgtgcggcac gccgacgagg acggcgggga tgttttcctt ttgcacggtt tcgatgattt
     3841 tcgcgatatt ggcgcgggtc tgctcctcgg gaactttgcg cagaaagtcg ttgccgccta
     3901 tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc gggcaggcgc gacagggctt
     3961 gcgcggacgt atcgcccgat acgccgccgt tgacaatatt ccaacccgtc agtttttgca
     4021 gttgcgcggg gtaggattcg tcagggtttg cgccgtagcc gaaggtgagc gaatcgccta
     4081 aggcaagcac ggtgcttcct tcgggaattt tggcgtgggt tcgggcggat tttctgccgc
     4141 aggcggtaag gagcaacgcg cccgcgccga ggaggaagat tcttctgttc attggttttt
     4201 cagacggcat cgagccattt cgggtggtat ttcagggttt cacggtaaac cggacagctt
     4261 tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa attcgccgac gatttcgccg
     4321 ccgacgaatt tgaaatgttt tttaaagagt ttgacccatt cgtctttgct tcgcggatgg
     4381 tgcgcgtcga gccagttctt gaacgagccg tattcttgtt gcaacgcttg gatttgccgt
     4441 gcattgaaaa tggcggcatc gattttcagg cggttgcgga cgatgcctgc atcggcaagc
     4501 aggcgttcga tgtcggcttc gtcaaatgcg gcgaccgtat cgatgtcgaa acctttaaat
     4561 gccgtctgaa acgcctgccg cttcttcagc atcagcatcc agcttaatcc tgcctgattg
     4621 atttccaaca ccagcctctc aaacaattca ttgtcgtcct caatcggaaa accgtattgg
     4681 gtgtcgtggt aatgtttgtt cgggttgtcg gtattttcgg gaagtgaggc ggcaaattcg
     4741 caatagttca cggtttactc gaccatctgt tgcgccgcct gtttcagtcc ctgttcgagt
     4801 gcggcggtca tggcggcgta gccgtcaccc tgctgttcgg tttcgatatg gaagggtctg
     4861 ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg ttttgcccgt gtagctgcct
     4921 tggaatgcgt cgatatagac cgtccatttt tcggtactgc cgctgcgtga ggcaggaaca
     4981 aagatgcgtg tgctgtccaa acggttgaat gcattgctca acgccgcttc gagcatatcg
     5041 tccaaggtgt ctgcccaaac gtggttttgt gcggtgttga tgcggtaggg gtcggtttga
     5101 tagaccagtc cgccgcgttt gagcggttcg gcaaggcgga cttcgacggc ggtttcgccg
     5161 ccttgcgttg caggacggat gtagcggctg tcgggcaaca cgaaatattg tgtgctttgc
     5221 acagtaccgc aggcggcaag cgtcagggcg gcggcaatcg ggaaaaggcg cattatcggc
     5281 ttcctttcgg gatagggtct ttgctgctgc tgttgaaaat cagcgcgttg ggtttttctt
     5341 tcaaagtgtt aatgacgggt tgaacgtctt ttaaggtttt gtccaaactt tgcagcgtat
     5401 tttgtacgtc gccgtagata ggcgattgcg gcgatatgcc ttgcagggtt atgcgcaatt
     5461 ctttcagggt ttggttcaac tcgttcggga tgttttgtgt ttgcggtttg ccgaccagtt
     5521 tgtcaatgga gcttagggca gcattggcag atttgagtgc ggatttgagt tcggcaagcg
     5581 agccgttcaa ttcggcaacg gttttatcca atggcagttt gccgaatttg tccagcaaat
     5641 ccgccaattt gacctgcaag tcgtccaaac cgccgccccg tgtggcaatg acggtatcgc
     5701 ctgcataaac ggtgtgcggt cgcagcttgg gcgaggtgga aggctgatcg ttcaactcaa
     5761 tcattttgct gccggtcagc aggttgttgc tggagatggt ggcggtcagg cctttgttta
     5821 aagccgtctg aaattgttgt ttccaatgct ctttgctttg ttcgttggca ttgatttcca
     5881 aacgggaagg ctcgatacgg atgcgtacgg gtatccagcc gttttcaaac aggtgcaggc
     5941 tgtcgttgcg gtcgaaataa ggaacgtcgg aaaccacgcc gacattcagg cctttgtatt
     6001 cgacaggcga accgacggtc agtccgcgca cagattgttt gaaaaacgcg gtgtagtaca
     6061 gcgagcggtc gtcaggcagg ttggcgactt cgctgcggct gtcgtaaagc gtgaagctgt
     6121 cttcgctttt aacgttttta ctgtttttgg ttttcggcga atcaaatgaa atcgcgcccg
     6181 acagcagggc gggcagaggg gcggaattga gtttgatgcc gctgcctgtg gtttcgatat
     6241 taatgccgct ttccaaccaa aaacggctgg cggaatgaat cagtttgtcg ttggggcttt
     6301 ggatgaagat ggtgtaatgc acgctttggt cggacgggtc gaaatgcgcg ctttcgattt
     6361 gcccgaccat aaagttttca tacaaaacag ggctgttgac gttgaggatg cggtcgtttt
     6421 tcccaatcaa attcaagcgc agtccgcttt gcccgatggc ggtaacgggc ggaatgtcct
     6481 gcacctggaa cacgtctttt gcttcgtcgc ttttgccggg tgtaaaagcg atgtacgaac
     6541 ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc gatacgcggc ttgaccaccc
     6601 aaaactgtgt gtcgctgcgg atgaggccgg atacgtccgc attgagttgg gcggttactt
     6661 ccacaccttt ctggtcgtcg cgcagtttga ttcgggtaac gcgtccgaca tcgatgctca
     6721 atactttgat gaccgtattg ttgacctcaa tgccttccgc gctgtccatc aagagcgtaa
     6781 ccacaggccc cctgttgcgg atttccttaa tccacagcca gccaccggca atcagcgcaa
     6841 tcagcgggac cagccagacg gcggagagca aggtgttgtt tttgcggacg cgtgcttggg
     6901 cgtgtctgtt tggaggaggg ctgttgtcag tcatattttt ccgtttcatt gaaagcaatg
     6961 ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga aataataggc ggacagcatc
     7021 gtcagaatca cgaccaggca gaaatagact gccgcactgc ccggaatgac gcgcgcggca
     7081 taagtgtgga acgaacacat caaaataata atcacaaaaa tatcaatcat cgaccagcgg
     7141 ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct ttgcgcccac cggcaaagcg
     7201 aaccgtgccg ccgcaatcaa aaccgccatt gccgcaatct tcagcaccgg caccaaaata
     7261 ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct cgtcccacat ataagcgatg
     7321 ccgttaagga tggtattgac ctccgtggcg gcaggattgg acgaaatcat aatcggcagg
     7381 atattggcag ggaaatacaa aataaccgcc gccgtcagaa acgccgacga aatactcaga
     7441 cttttcggcc gtcggcggta cagttccgca ccgcacacac cgcaggggga ttcggcactg
     7501 tcgcggaaat acaggcagcg gctgcaacag gttttgtctt ctgatgccgt ctgaaccgca
     7561 ttatcccccg tcagccgccc gatttgaaaa tacacccaat gctggggaac ggataccgaa
     7621 gtccgaatca gcataaccga cagcgcgaac atcagataaa acgccgaccc gaagcgaacc
     7681 tctgccacag acgagagctt gatatacgcc accagagtgg aaacaaaaaa cacatccacc
     7741 atcatcgcct gcctcaagcg caccattacg cgcgttgcca aacgcagcgc aggatacgcc
     7801 tgtttccgta tcagcgcggc atagacatac aggcacagca gcagaaacag aaccggcgcg
     7861 ccgaaggtca gcacaaacat cacttcggct aaaaaaccat aatcctgaaa caccatcagg
     7921 cgcatcatct cgggcagcga aaggacggat gccgcacccg gtatcccgac ctcgatatac
     7981 gtcataccgt aagcaaacgc catcaaaatc agcgaagccg ccgcataggc gggcggggcc
     8041 gaaaaaggat gcctgcccac cctgaagagt ttgtgtccgc aactgggaca gaacgccgcc
     8101 tctccactgt ccaaccgagg tacatccgcg cggcagccgc attccggaca atctaccgta
     8161 tgcggcggca gggcttcgtc gcggcagttg ccgcgaaggc tgtgggggat attttcggca
     8221 aacggtttca tcggaaaagt ggcaaatata agtggcggca ttataaagga aaaaaagcga
     8281 tacacccaat caggatgcaa aaccggcagg ttcggcagta tgaacaaacc cttgtcgtcc
     8341 ggcaaaaata ttttttcgag cggctgtccg ccgactgccg aacccatttg ataaaatccc
     8401 gattctgtaa agaatagtaa aatacctgct taaaaacatc acgcaaatga tattttttcc
     8461 aaaaatatgt gataataaat ctcgtttatt atttttgtat gcggtcaggt acggattttg
     8521 cccgcaccgc atccttatcc cttaccgaca aaggaaaagc tatgagcgaa accgaaaatc
     8581 aagccctgac gtttgccaaa cgcttgaaag ctgataccac caccgttcac gacagcgtgg
     8641 ataacctcgt tatgtctgtc caaccgtttg tcagcaaaga aaactacatc aaatttttga
     8701 aactccaatc cgttttccac aaagccgtcg atcatatcta taaagatgcc gaattaaaca
     8761 aagccattcc cgagctggaa tatatggcgc gatacgatgc cgtaacgcaa gacttgaaag
     8821 acttgggcga agagccttac aaattcgaca aagaattgcc acacgaaacc ggcaacaaag
     8881 caatcggctg gctttattgc gccgaaggat ccaatttggg cgcggcgttt ttgttcaaac
     8941 acgcccaaaa actcgattac aacggcgaac acggcgcgcg ccacctcgct ccgcatcccg
     9001 acgggcgcgg caaacactgg cgcgccttcg tcgagcatct gaacgccttg aacctgactc
     9061 ctgaagccga agcggaagcc atccaaggcg cacgcgaagc ctttgcattc tacaaagtcg
     9121 tgttgcgcga aactttcggc ttggcagccg atgccgaagc accggaaggt atgatgccgc
     9181 acaggcattg atatatttta cggtttcgcc tcggctcgtg aagcggaacc gtattttctg
     9241 ccgcgtatga cggcattttt caaaccatca gatataaata attatcatat tttttattaa
     9301 aaatatgcca ttaggagata attatgacga ttcctttcaa accaagcctg cttgcaatgg
     9361 catccgtcct tgccctttcc gcctgtattt caggaggagg ggatgcgcct gcggtcaaat
     9421 cctcggaaac accgtcaaat aaagttgccc cccccactga tacggcaagc cctgctacag
     9481 gtgctgatac ggcaagcccc tcggcaggtg gggatggcgg ggattctaaa acagaaatac
     9541 cgcctaaaga tacgcaaggg gaaggcgcgc cggcaggcac tgatggcgga gtgcaaaatg
     9601 atgcgtccca acataatgat aatgcgccgg caaccggcac acccgagacg cagcctgaaa
     9661 acaaccgtat gcccggcagt gatgccgtag ggtcggatgg cggcaatcaa gataccgatg
     9721 cgccgaaaaa tacggaatct caagatggta cggaagaaaa cgctgtcagc gaaggcacag
     9781 ggatgccggt aagccctcct tctgaaaacg ccgaaaatac tgaggttgcg atgaatgccg
     9841 aagatgcaaa cccgccgtcg aaagccgaaa attctgatga ggtggggcaa ggcgggatgc
     9901 aggctgatga cggcaggcag gatgcgaaag agggattgca aggcgatgac ccggctgctc
     9961 attcccccca agatcaagac caacaaccac aaaatgctgc cgatactcaa cctaaaacag
    10021 gcgtgtttgg cgagaaatat tttgacggta cgggagaaat cacgacgttg gtgattggtg
    10081 gggaaaccat tgacttaacg aaagctgatg agccggtatc aagtagaaat caccccggtt
    10141 tgatgagtga tagtgatttt gagaaggtaa tggtttctca agggcaatat cagcatctga
    10201 tttatggggc ggctaaatta caagacggta catacaccct ttttgtacaa ggtaagccga
    10261 cagaaaattt gccgacagga atgatggcta aatatcaagg taaggtgctg cattttgttg
    10321 gaaaatataa ggataattgg tttaataata gtgcgtatag agccgcagga acatttacgg
    10381 cagatgttga tttctcacgg aaaaccttaa caggaaaaat tgataccaaa gatccttcag
    10441 gtatggggat gcaagaattt actgcgaata tcgatggaaa tggatttcaa ggtaagtgga
    10501 aatcgaataa tgatcaaggc gatatttccg ggaaatttta tggtcaagat ggcgcggaaa
    10561 tggcaggcag atacagctat aaagataaca atgagcataa gagcggcttc ggcgtatttg
    10621 caggcaaaaa agaagcaaaa gcccgttgat atttatcctg tgtttcacag tcggaaacag
    10681 tggcgttttc ttcagacagg gcatcgggca acaccttaat gtgttgtccc acctgcagaa
    10741 gcaatcctaa ttgaacgatt atctgtatta ccgatttgat ttataaacta tgccgtctga
    10801 aaaatgtttc agacggcatt ttttgattgc cgatatgttt aacggacgat gaccacgcgg
    10861 acgtggaacg agcctgcctg acagtcgtat tgtccgcctt gcagcggctg cttgtcgaaa
    10921 tagctgagga agccggtaac gccgcgtccg caagggtggc ggcagatgcc gcattgtgtt
    10981 tataatgccg tgtcagacgt ttgatttatg ggaaaataat gatgcttacc aatcaggaag
    11041 taaccggtgt caaactggga taccgcaaag ccgttttaca agagctggcc cgggatttcg
    11101 ggggcagtgt gcacagtatc gtattggatg cggtggatgg tcatatagaa cgcttgaaag
    11161 ccaaacaagc ctacgataag cgggcgatgc aggcttttca ggattatcgg gaaacaggat
    11221 tgcatattac gcttaatgag tttgcagact gggtggacgg cttgggtggc gatgaatcga
    11281 aggatatgcc cgaatgccgc aagtaaagct gataattaca cgccccgctc aatctgattt
    11341 ggtcaggatt tataattttt tgaatgattt gggtgcggaa catcaggcaa atacagtgat
    11401 gcggctgctt aaaaattctt ttgtttctac acaaggcagc ccgagtaatg ggaaacctta
    11461 tgatttgtcg gtggcggggg aaactttgca aaatgtaagg gaagtaatcg tgccttatgg
    11521 caaaagcggt tatagctatt tgttttggca cgataaatca ggtaagcaaa tattgatttt
    11581 gacggtgagg catttccgtg aaaatgccta ccgcctgagc tttccttttt tataacggta
    11641 tttggtttgg cggctttcac ttgtgcgggg caatgcgttt cggctttgtt gcggcacgtt
    11701 cggataaacc gccttcaaga acataaccgg actgccgctt tgattccgtt ccgcaggcgg
    11761 gatgtttgaa cggtgcagat atggaagtat ggagtgtccc gatctgaatc tgccgcacaa
    11821 taaggcaagg ggattaaaaa tgccgtctga aaaatgtttc agacggcatt tttggtcgcc
    11881 gatatgttaa cggacgacat tggcgcgcat gaccacgcgg acgtggaacg agcctgcctg
    11941 acagtcgtat tgtcctcctt gcagcggttg cttgtcgaag tagctgtgga agccggtaac
    12001 actgcgcccg ccgagtttgc cggcggtctg ctggaatttg cgggcggcat tgattagggc
    12061 gcgttcgcag gcctcggagt cggatttgcc tacgccgttt gctgcttgcc gtgtaaccag
    12121 attcctgccg tttgcaaccg gcacgccgcc gccgccgaag ctgacgggaa tcccggattc
    12181 caatacgccg tctgcaatgg ctgcatttat tgccgcctga ccgttgctgc aacggtaaat
    12241 gatgtttttg ccgcttcctg cggtgtctcc gcggatgact ttgcatgatt ctccggccgg
    12301 tgtgccggcg gatgccgtgt gcgcgttttt aggtgcgcgg ccagattggc aggctgccag
    12361 catcagggcg gcggcggaga gtatgaggat ttttttcatg tcggatgtcc ttgtttgctg
    12421 tgggaaatca ttagcattgt accgtaagat tcaacaaaat gccgtctgaa aacggggttc
    12481 ggacggcatt gtggctcagg ggttcaacac gcgtgcgatg agggcttttt ctttgccctg
    12541 catattgggg attttgactt gaatgccgtt gcctggtgcc acatcgacag gctgaccttt
    12601 gcgggtcatt tgttccaatt tgatggtttg gttgccgctc gggtggatga tttcgagtga
    12661 atcgccgatt gcaaagcggt ttttaacttc gatggtcgcc cagccgtttt cgtcgatttc
    12721 ggtaacgtgt ccgacgtatt ggctttgttt ggcggtggaa tggccggtca ggtagttttg
    12781 gtaatcctga gtttggtggc gctcgaggaa gccgctggtg tagccgcggt tggcgaggcc
    12841 ttcgagttcg ctcaacaggc tgtaatcaaa cggacggcct gcgacggcat catcaatcgc
    12901 tttgcggtag gactgggcga cgcgggcgac gtagtagagc gacttggtgc ggccttcgac
    12961 tttgaggctg tccacgccga ttttggcgag tttttcgacg acttcgatac cgcgaaggtc
    13021 tttggagttc atgatgtagg tgccgtgttc gtcttccata atcggcatca tctcgcccgg
    13081 acggttggac tcttcaatca ggaagacttt gtcggcatag gggtggcgtt tttgaccgtt
    13141 gatgccttca aagttttggt tggcttcttc ttgggctttt tcaaagttaa aaccttgcag
    13201 aagctgggcg tcgcccgcat cgctttcagt ggcgttgtga accttgtaat cccaacggca
    13261 ggagttggtg caggtgcctt ggttggggtc gcggtggttg aagtagccgg acaacaggca
    13321 acggccggag taggcgatac acaatgcgcc gtgaatgaag acttcgagtt cgatgtcggg
    13381 gcattcttgg cggatttcgg cgatttcttc catactcaat tcgcgcgaca ggatgatgcg
    13441 ttcgacgccg atgttctgcc agaatttcac gccccagtag ttggtggtgt tcgcctgtac
    13501 ggacagatgg atcggcattt ccggccattt ttcgcgcacg gtcataatca aacccggatc
    13561 cgccataatc agcgcgtcgg gtttcatggc aatcaagggc tccatatcgg aaacgaaggt
    13621 tttaagtttg gagttgtgcg gcagcgtatt gacggtcagg aagaattttt tgttgcgctc
    13681 gtgcgcttct ttaatgcctt gctcgaggac gtcgagtttg gcaaattcgt tgttgcgggc
    13741 gcgcagggaa taacgcgggc tgccggcgta aacggcatct gcgccgtagt cgtaggcggc
    13801 gcgcatacgt tcgagaccgc ctgctggcaa taagagttcg ggtgctttca tcgcgtttcc
    13861 ttttcggttg aaaccccgcc ctttagggcg gcaggatcag actttatttg ggaggggcgt
    13921 aaccccttcc gaatcagggc aacatatagg gcgacgcttt atgtgtcgtc ctgtgtgttg
    13981 aaacatgatt tgtgtgcttt gtaaaaatgt ttcagacggc attgctgcaa atgccgtctg
    14041 aaaagggttt gagaatgggc ggattatccg ccttttcgtg ttcgcggtca attttgccgg
    14101 gaaatttata aggatttatc aagtatttgc ctgcttggga tacaatgcgg cagtttgaat
    14161 gcgagagaga atgtgatgaa tcctaaaatc gtgtttttcg acattgacga tacgctgtac
    14221 cgaaaatata cggatacctt gcgcccttcc gtgaaaacgg cggtggcggc tttgcgcggc
    14281 aaaggtatat tgacggcgtt ggcaacgggg cggtctttgg cgacgattcc tgaaaaggtc
    14341 agggacatga tggcggaaac gggaatggat gccgtggtaa cgataaacgg gcagtttgcg
    14401 ctgctgcacg gaaaaactgt gcgcgaagta ccgatggatg ccggtttgat gggcagggtt
    14461 tgcgcgcatt tggatggctt gggcatggat tatgcgtttg tcggcggaga ggggatcgcc
    14521 gtgtccgcgc tgtcggaatg cgtgtgccgc gccttgaagt atatcgccag cgattttttt
    14581 gccgataagg attatttctc aagcaaaccg gtgtatcaga tgctggtgtt tgcggaggaa
    14641 aacgaaatgc cgctctggtc ggatattgtg gaacaggaag gcttgaaaac ggtgcgctgg
    14701 cacgaggaag cggtcgatct gctgcctgcg ggaatgtcga aaacggacgg catcagaagc
    14761 gtggttgaag cattgggatg gggaatggcg gacgtgatgg cgttcggcga cggtttgaac
    14821 gatgtggaaa tgctgtcgga agtcgggttc ggcgtggcaa tgggtaacgg ggaacaggcg
    14881 gcgaaagaag cggcgaaata tgtttgcccc ggcgttgatg aagacggcgt gttgagaggc
    14941 ttgcaagaat tgggcgtgat ttgaacgcat catcaaaacc ctgccggttt cagacggcag
    15001 ggccggttgt ttcagccttt catacagcct tcgttttgaa gcagggtaaa taagggcgcg
    15061 ccgcttgcgc ggatattctt gccgccttga aggtcggtaa attccaaaat ggcggcggct
    15121 tcgacaattt ccccgccgag tttgcggatc agctccagcc cggcaagcat cgtgccgccc
    15181 gtggcaacca aatcatcgac cagcaggacg cgcgaaccgg gtttgaccgc atcggtgtgg
    15241 atttccaccg ccgcttcccc gtattcgagc gcgtagcttt gcgatacggt ttcaaaaggc
    15301 agcttgccct ttttgcggat ggggacgaag ccgacgttga gctggtaggc gagtgccgcg
    15361 ccgataatga agccgcgcgc gtccaaaccg gcaacgatgt cgattttctg atccatatag
    15421 cggtaaacca gcagatcgac caaaagacgg aagtattccg cgctttgcag gacgggcgtg
    15481 atgtcgtgga ataagatgcc tttttgcggc cagttttcga ttttgcggat tttgtcggca
    15541 agcgcgccga cactcatagc ttcgggatga accagcattg cgtgttccaa gtttgatgtt
    15601 tttaacgcca tattgtagcc gtcagcctgc cgcttgccaa atcaatcaaa acaatagcgg
    15661 caaaatatgg gcatcggcaa atatgaaacc gggtgtccgt tgtgatacaa tcggtcggaa
    15721 aatatacagg caaaaaatat gtccgcttat cgaaaaggca acatctttat catttcggcc
    15781 gcttctggca caggcaaaac cacgctggtg tcgcggctgt tggcaaacca taacggtttg
    15841 cgcgtttccg tgtcgcacac aacgcgcccg ccgcgtgaag gcgaagcaaa cggcgtacat
    15901 tatcactttg tttccaaaga agagtttgag tcgcttatcg cgcaggaagc ttttttggaa
    15961 tacgcagacg tattcggcaa ctattacggt acaagtatcg agggtgtgaa tgccttggct
    16021 gcggcaggct atgacgtgat tttggaaatc gacgttcagg gcgcggcgca ggttcgcgat
    16081 gcgctgcccg aagccgtcgg tatctttatc ctgccgccgt ctttcgatgt gcttgccgct
    16141 cgcctcaacg gacgcgggac ggacagccgg gaagttatcc aaaggaggct gtcgaaggca
    16201 aggcatgaaa tcgaacagtc tgtgttgttc gattatgtcg tggttaatga cgacttggcg
    16261 cgggcggagg aagatttgct ccatattgtg aatgcctgcc gtctgaaaag gtcgcggcaa
    16321 ctggggttta ttgcagattt gttggaaaat tcctagaaaa cggcgaaaat acccggtttc
    16381 ccaatttaaa tatttttgaa agaaagcaaa taatatggca cgtattacca cagaagactg
    16441 caccggaaaa atttccaacc attttgacct gacattggta gcggcccgcc gcgcccgcca
    16501 gcttgaaaac ggcaacacgc cgcttgtgga cgatgtccgc aataacaagc cgaccgttac
    16561 cgccttgcgg gaaatcgccg ccgggcatat cggtacggaa ctgctggcac gcaataaata
    16621 aatcctgccg gaaacgcacg ccggcatatt ctgccgccgt gcagtccgac ggtttgaaat
    16681 gaaaaaacac ataccgaaag ccgtctgaaa tgcccgcccc ccaaccttcc gccccttacg
    16741 accccctgac tgccgaagcg cgtaccctgc ttttccatac cgcctcctat ctcaagcccg
    16801 aggaacaggc ggagcttgaa aaagccgtcg cctatgcgtt tcgcgcccac gacgggcaaa
    16861 cccgtaaaag cggggaaccc tacatcacgc acccgattgc cgttgcaacg cagctcgccc
    16921 tttggcatat ggatatacag ggcctttgtg cgggcgtgat gcacgacgta ttggaagata
    16981 cgggcgtgac aaaaggggaa atggcggcgg tgttcggcaa tacggttgcc gagatggtgg
    17041 acgggctgtc caaactcgaa aaactcaaat ttgaagatca tgcagagcat caggcggaga
    17101 gtttccgcaa actgattttg gcaatgacca aagatgtgcg cgtgattgtc gtcaagcttg
    17161 ccgaccgcct gcacaatatg cggacgctcg gttcgatgcg tccggacaag cgtcgccgga
    17221 ttgcaaggga aaccctcgaa atctacgcgc agattgccaa ccgcataggt ctgaataatg
    17281 cgtatcagga gcttcaggat ttatcgttcc aaaaccttca tcccaaccgc tacgagactt
    17341 taaaaaaagc aatggacaag agccggaaga accggcagga cgttgtcggc aaagtcttgc
    17401 gcgcattcgg tcagcggctg gtaggcgcga atatagaggc caaaatcaaa ggcagggaaa
    17461 aaaacctgta cggcatccat cagaaaatga tggcgaaaaa gctgcgtttt gccgaggtta
    17521 tggatattta cggtttccgc gtcgttgtca acagtattcc cgcctgttat gccgcactcg
    17581 gcgcattgca taccctctat cagcccaagc ccgggaggtt caaagactac atcgccattc
    17641 cgaaaagcaa cggttatcaa agcctgcata ccactttggt cggtccttac ggcttgccga
    17701 ttgaagttca gatacgcacg cgggaaatgg atgccgtagc ggaaggcggt atcgccggcc
    17761 attggagcta taaatcagat tcccaaacgg tcgatcaggc ggtactgcac accaatcaat
    17821 ggttgaaaaa cattttagac ttacaggcaa gcagcgccaa tgccattgag tttctcgaac
    17881 acgtcaaagt cgatttgttc ccgaacgaaa tctacatcct taccccgaaa ggaaaaattc
    17941 taactttgcc caaaggggcg acgcctgtcg attttgctta tgcggtacat accgacatcg
    18001 ggcataaaac cgttgccgcg cgtgtcaata acgtgatgat gccgctgcgt acgaagctca
    18061 aaaccggtga ttccgtcgaa atcatcacat ccgaacacgc caaacccaat cccgcgtggt
    18121 tgaatttcgc agtgtccggc agagcgcgaa gcgccatacg ccaatatatt aaaaacctta
    18181 accggtacga tgcggtcgtt ttgggagaga gccttttaca aaaagccctg tccagtttgc
    18241 tgcccaaaga tgtcctgctt tcagacggca tcaaagaaaa gtatttgaaa gaccttaacg
    18301 acaagcagac atcgtttgaa gaagtgttat acaacgtagg gatggggcat accctgccgg
    18361 tttatgtcgc tatgcatatt gccgaattgg caggaaagca tttcggtagc gaggtcaggc
    18421 tcagttccat taaagtcgat ggtcaggaaa gcgggcatat tcattttgca gagtgctgcc
    18481 accctgttcc tggcgattcc atccgtttgc tgttggttaa gggaaaaggc atgattatcc
    18541 atagggatag ctgcccgacg ttgttgaagt ccgatcccga acagcagctg gatgcagact
    18601 gggaaaatat gaatgggcag aactaccgtg tcgggcttca agtccaatcg gaagacagcc
    18661 acggcctgtt ggcattaatg gcgcaagcga tttccgattc cggtgcggac atcgagtcgg
    18721 tcgaaacacc atccaaatcc cagtcgggaa cggaaggttt tgtcgaattc aaattcttat
    18781 tgaaagtcaa gaatctgaat cagttgaatc agattattca aaatctgcat agtattccat
    18841 atatccgcaa agtcatcaga agttgaagca ggtaaaacgg gttgcctttt gattaacagg
    18901 caacccgttt tatggtttat ccgttcaatt gcttatttgt tcaaatatac ggcaagcctt
    18961 tcaacaatct gcatcgccgc ttcatcttta cttgtttcgg gaaaagatag ttccccgtcg
    19021 tccccgataa tggtaatccg gttggtcggt ttgcccatcg cgattgaaac atcattggca
    19081 actattaccg gtattttttt cttaatacgt ttttctcgtg catatgccat tacattctcc
    19141 gtttcagcgg caaaaccgat gcagaacggc gggttcggca atgaggcaat agaagccaaa
    19201 atatcagggt tctcatccaa ttcgatggat agccgtttgg catttttatc ttttttgaat
    19261 ttttgcgtac tcctgttttt aaccttatag tctgagacgg cggcaacaga aataaaagca
    19321 tcttgttttt caatcaaacg atgcactgcg cgatgcatat cttcagcgct gacggcttga
    19381 accgtatcgg atatgccgaa aggcagttcg gtttgaagct gtccgtgaat caggctgact
    19441 tccgcaccgg cggcacggca cgcccgcgcc aaagctaccc ccatttttcc gctggaggta
    19501 tttgtgatgc ccctgacagg gtcaatggct tcaaatgtcg cacctgcggt aatcaagatt
    19561 ttcttgcccc ttaaaatttt cggtgtccac aagtccggaa gcaaatccag caattcggca
    19621 ggttccggca tccttcccat accattttct ccgcaagcct gttcgcccga gcccggcata
    19681 tagacagtaa tgccgtctga aaccagttgt gcgatattcc gttggttggc agggttgagc
    19741 cacatttcca cattcatcgc gggagcgatg gcgagcggac atttccgtgc ggctgccaga
    19801 ctggtcagta ggttatctgc cacgccgtta cagatttttg ccacggtatt catacttgcc
    19861 ggcgcaatca gaaaaacatc cgcattccgg gtcaggttga tatgttccat accgtttgaa
    19921 ccgttgccgc cgtgcgtgtc ggtcaggaca ggattgccgc ttaaagcctg aaaagtcagc
    19981 ggagaaacaa attcagttgc cgagcggctc ataaccaccg taaccgaatg cccctgtttt
    20041 ttcagcagac gaaccaactc gcaagactta tacgccgcaa tactgcccgt tacacctaaa
    20101 agaatatgtt tacccatttt gacaatttct aattacgaag aaaaaacagc agccattcta
    20161 taacaaagtg cggatacatt agttaccgtt tgga
//
LOCUS       DAXIEB010000027        19927 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000027 DAXIEB010000000
VERSION     DAXIEB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.027"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            85..885
                     /locus_tag="ACFOA3_000805"
     CDS             85..885
                     /locus_tag="ACFOA3_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6897837.1"
                     /translation="MNPAPKNLLFSSLLFSSLLFSSLLFSSAAQAASEDGSRSPYYVQ
                     ADLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYA
                     SYRKWKESNSSTNAENRDNAQNYVKIETKHQGNGSFHASSSLGLSAIYDFKLNDKFKP
                     YIGARIAYGHVRHQVHSVESKNTIITSKPKNGAAQGGPVPTGFVGKPAYHESNSISSL
                     GLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(859..1068)
                     /locus_tag="ACFOA3_000806"
     CDS             complement(859..1068)
                     /locus_tag="ACFOA3_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897838.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(1086..1643)
                     /locus_tag="ACFOA3_000807"
     CDS             complement(1086..1643)
                     /locus_tag="ACFOA3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6897839.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1719..3353)
                     /locus_tag="ACFOA3_000808"
     CDS             complement(1719..3353)
                     /locus_tag="ACFOA3_000808"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6897840.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKEFLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3423..3757)
                     /locus_tag="ACFOA3_000809"
                     /pseudo
     CDS             complement(3423..3757)
                     /locus_tag="ACFOA3_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3757..4863)
                     /gene="serC"
                     /locus_tag="ACFOA3_000810"
     CDS             complement(3757..4863)
                     /gene="serC"
                     /locus_tag="ACFOA3_000810"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6897841.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYVIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5228..5659
                     /gene="rimP"
                     /locus_tag="ACFOA3_000811"
     CDS             5228..5659
                     /gene="rimP"
                     /locus_tag="ACFOA3_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6897842.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIKGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5687..7189
                     /gene="nusA"
                     /locus_tag="ACFOA3_000812"
     CDS             5687..7189
                     /gene="nusA"
                     /locus_tag="ACFOA3_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6897843.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            7201..10089
                     /gene="infB"
                     /locus_tag="ACFOA3_000813"
     CDS             7201..10089
                     /gene="infB"
                     /locus_tag="ACFOA3_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6897844.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNSSTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPIAAETKPAEESKAEKAQADKMPSKKPAKPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKSAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTQHEVIPDDWGGTVQFIDISAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(10193..11533)
                     /locus_tag="ACFOA3_000814"
     CDS             complement(10193..11533)
                     /locus_tag="ACFOA3_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HFC6897845.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRIGTGAATGALIGLGLDIATFGGSLGLGTAI
                     GGFLGGILPNTRTISDKLTGRQTLHTDPETLTLLAARALGLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGK"
     gene            complement(11595..12944)
                     /locus_tag="ACFOA3_000815"
     CDS             complement(11595..12944)
                     /locus_tag="ACFOA3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6897846.1"
                     /translation="MLNPSQKLVELVRILEEGGYIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKIFLKTSQ
                     SKLDLEKPYYQAVIRRWQNKITDADTHQETVSAVSPKIILNDAPKWAVMLETGWHEGT
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLAEKLEHWRNALTECGAAWLEPDRVAQEVRLKTNDRT"
     gene            13088..13714
                     /locus_tag="ACFOA3_000816"
     CDS             13088..13714
                     /locus_tag="ACFOA3_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6897847.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSI
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13778..15196)
                     /locus_tag="ACFOA3_000817"
     CDS             complement(13778..15196)
                     /locus_tag="ACFOA3_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6897848.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            15523..16251
                     /locus_tag="ACFOA3_000818"
     CDS             15523..16251
                     /locus_tag="ACFOA3_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6897849.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16310..16798)
                     /locus_tag="ACFOA3_000819"
     CDS             complement(16310..16798)
                     /locus_tag="ACFOA3_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6897850.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17545..18009)
                     /locus_tag="ACFOA3_000820"
     CDS             complement(17545..18009)
                     /locus_tag="ACFOA3_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6897851.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            18231..19289
                     /gene="alr"
                     /locus_tag="ACFOA3_000821"
     CDS             18231..19289
                     /gene="alr"
                     /locus_tag="ACFOA3_000821"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6897852.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5289 a         5465 c         4986 g         4187 t
ORIGIN      
        1 gccccaaacc tgatatagtc cgctcctgca acatcattga aaattgttct ttttaatcag
       61 ttaaaaccga atacggagtc gaaaatgaat ccagccccca aaaaccttct cttctcttct
      121 cttctcttct cttctcttct cttctcttct cttctcttct cttccgcagc gcaggcggca
      181 agtgaagacg gcagccgcag cccgtattat gtgcaggcgg atttagccta tgccgccgaa
      241 cgcattaccc acgattatcc gaaagcaacc ggtgcaaaca acacaagcac agtaagcgat
      301 tatttcagaa acatccgtgc gcattccatc caccctcggg tgtcggtcgg ctacgatttc
      361 ggcgactgga gaatagcggc agattatgcc agttacagaa aatggaaaga aagtaattct
      421 tctactaatg cagaaaatag agataatgca caaaactacg taaagattga aacaaaacat
      481 caaggaaacg gcagcttcca cgcctcttct tctctcggct tatccgccat ttacgatttc
      541 aaactcaacg ataaattcaa accctatatc ggcgcgcgca tcgcctacgg acacgttagg
      601 catcaggttc attcggtgga aagcaaaaac acgattatta cctctaaacc aaagaacggc
      661 gctgcacagg gaggccctgt cccaacaggt tttgtcggca aacctgccta tcacgaaagc
      721 aacagcatca gcagcttggg tcttggtgtc atcgccggtg tcggtttcga catcacgccc
      781 aagctgactt tagacaccgg ataccgctac cacaactggg gacgcttgga aaacacccgc
      841 ttcaaaaccc acgaagtctc attgggcatg cgctaccact tctgattccc cgataccgat
      901 gccgtctgaa ccttcagacg gcatgagacc tttgcctgcg tacttggtac gctggtcgcc
      961 tccgaacatg gcgcgacacc cgacatttcc gccgaacgca tcgggcgttt catgaatccg
     1021 gtttaaaacg catggaaaaa tgccgtctga aagcctttca gacggcattg tgcttgagat
     1081 tccgtttacc aatggctgac aaacgcttcc aaatcggtat tcttgggctt atgcacttcc
     1141 tctgtcggcg tgccgaccat catcagcccg atgattttat ccttatccgc acaaccgaaa
     1201 gcctcccgca acaaggggct gttgacccac atccccgtaa tccagacatt gtcgaatccc
     1261 tgagccgttg ccgccagttg cagcgcatac gccgcacaac ccgccgtcag catctgctcc
     1321 cattccggtt tcggcttagg cacatcacga ttcggcgcaa acgttacccc gatgaccatc
     1381 ggcgccatat tgcccacttt ttccgccttt ttcatcgcat cgtcgccgaa attcaattcg
     1441 gcaaccgttt gctttaacac atcgcgaaaa cgttgcaatc ctacctcgcc ttgaatcacg
     1501 gtaaaacgga aggggcgcat attgccgtga tcgggaactt gggttgccgc ctgaaatatt
     1561 tgttccaact ccgccgcatc gggggcgggg ggcttcagct ttttggaaga tcggcggttc
     1621 gtcaataatt ttaaagcatc catatcgtta tactccggtc atcggtcggg tgttcggaca
     1681 catatgccgt ccgaaggctt cagacggcat atccggcatc agcgcggacg gcggcaggct
     1741 gccaatatat ccatttcctt ccgataggtt tggctattgg aaatgtccat cagccccaat
     1801 accgtgctga aatagtggtc gtgcgaatat tcgttttccg ccgctttttg tttgaggcat
     1861 tggaaatcta tgcccccgtg ttggcggaag gctttggaaa accacataac catcgggata
     1921 tgcgtctgcc cggaaggcgc gatggcgtaa ggcgcggcgt gcaggtacat cccgttttcg
     1981 cccaaacttt cgccgtggtc ggaaacataa tgcaccacgc tttccaaatc gtcgcggttt
     2041 tcaagtttgc ggataacctt gtcgataaac tggtccacat acaaaaccgt attgtcgtaa
     2101 gtgttgacca gcgtggcgcg ggtgcatttg ttgatttcgt tggtgtcgca ggtcggcgtg
     2161 aatttgcgtt cggcttcggt atagcgttcg taatacgtcg gcccgtggct gccgatggta
     2221 tgcaggatta aaaccgcgtc tttatcgttt ttgttgagga cttcgtcgaa cttagtcagc
     2281 aggatattgt cgagacactc gccgttgcgg cagtattcgg gcaggttgag cgaggtaacg
     2341 tcggtattcg gcactttgcc gcacacgccc ttgcagccgg aatcgttttc caaccaggta
     2401 acttccacgc cggcgcgctg cacgatgtcc agcaggttgt cttggtgttc ggctttgatt
     2461 tcgtcataat ccgtgcggtc gaaggttgag aacatacacg gcagggagtg cgcggtcgat
     2521 gtgccgcagc ttctgacctg cgggaaattg acaatttcat cgccgcgcgc ggcaagcagc
     2581 ggcgtagttt ggcggctgta accgttcaaa ccccagttgg cggcacgcgt ggtctcgccc
     2641 acgaccagca ccacgaaacg gcgcaggctg ccggccggcc ggttttgcac gaccgccata
     2701 tccaattgcg tataaggaat attggaacgc ttccaatctt tgtatttcga cacgcccgcg
     2761 ccgatgaaat tagacggcac aatcagatgg gttactgatt tattgttgcg gaaaaacgag
     2821 gcgtaatcct gatattgcaa cattgcgatg cccaacgcgc acaaaaagga aacggcggca
     2881 agcacaaggc gcgtcaaaaa ctccttatac caaacgcggt atttaacctt gacggcgata
     2941 tacgccagcg cgggcaatac gcccaaacat acaatccaca gcacatagcc cggcgtaatc
     3001 aggcgtgcgc tttcggcagc cgtagtttgc aagacattat tcaacatcga cttgttgaaa
     3061 tagatattga aaaatatttc ttggtaagac accgccgcac tgataaccaa tatcaacgga
     3121 atcaatacct tatgcacgaa aggcagggca atgacgtgaa aaacgaaatt acttaaaaaa
     3181 aacagcacca ccggcatcgt atagaggaag atatccgccc cggtgccgtt aaaaggatga
     3241 agctcgacaa ctttggcaaa aaaggcgtaa ttcaatacca gcgaggaata cagggaaagg
     3301 aaggcaatca gcgcggaaga gccgagcttc ggcctcaggt tcggttttat catttggaat
     3361 gtgtcggata agggttggaa aaggcatccg gcatttggaa tccggattat tgaaaaagat
     3421 tcttaattat aaggcaacgg agcaaagcag ggcaagaaaa cggcggctgt gcgggggggt
     3481 tccgcccgcc attcaaacgt ccggcagaca taaaaacatc gtaagcaaga ttcgaaccgg
     3541 tctgcaaccg cccctgccag aaaaacgggc aaagcatttc atattggaaa aacccggccg
     3601 cgccgccgac gggacagtcc ggcacaaaca gcatcacgcc cggattgaaa aggacggata
     3661 gccgccggca gcacaatctt accacctcca aaacgccgcc ggagaacgcc gaaacagccg
     3721 atgccgtctg aagccgcatc agacaacatc gggacatcaa ccgtaacgcc gttggaaatc
     3781 gcgcataaaa tctgccaaag cccgcacgcc ttcaagcggc atcgcattgt agatgctggc
     3841 gcgcataccg ccgacggttt tatagccctt aagcaggcac aagccctgca attcggcttc
     3901 cagcacaaaa cggcggtcaa gctcctcatc ccccgtttgg aacacgacat tcattttaga
     3961 acgcgcattc ggacggatac ggttgatata gaaaccgccg ctgccatcta tcgtctcata
     4021 caaggtttgc gccttcagcc gattgaccgc ttcaattttt ttcacaccgc cctgcgcctg
     4081 cagccagcgg aacaccagcc ccgacatata aatcacgtaa gttgacggcg tgttgtacat
     4141 accgtcgcgg ttgatgtgcg aacggtagtt gaacacatcg ggaatatcgt tcggacaacg
     4201 ctcgagcaaa tcctcacgca caatcaccac cgtaacccct gccggcccga tgtttttctg
     4261 tgcgcctgcg taaatcagtc cgtagtcggc aacatcaaac tcgcgcgaca aaatctcgct
     4321 ggacatatcg cacaccagcg gcggcatgcc ttctgaaagg cgcggcactt cacggtattg
     4381 cagcccgttg accgtttcat tgacggcaaa atggacaaac gccgaatcgg gtgcaacatc
     4441 ccacgtttcc acaggcggca ggtcgagata gtcgaactgc tcgccgccat gcgccgccaa
     4501 acggatttcc gtatcggtca aacggctcat ctgttcataa gcgatacggc tccagttgcc
     4561 cgttaccacc gcgtcggcag tgcggaaacc gtgtgccaga ttcatggctg ccatattaaa
     4621 ttgggttgtt gctccgccct gcagaaacaa tatcttatag ttgtcaggca ctttcaaaag
     4681 ctgcctcaaa tcctgttccg catgatgcag gatgctcaaa aacatttccg aacggtggct
     4741 catcgccatc acgggaaaac ccgtaccgtt gtagtccaac atttcctgcc gcgccgtttc
     4801 caacacggct tcgggcaata cggcagggcc ggcggaaaaa ttgtaaatcg gataaagaga
     4861 cataatgcag ccttgattct gaacaataac ccgcccgatt ttaggcttgc agcaggcttt
     4921 gtgcaaggat gggaaatacc tgtcctccgc ccgattccat gccgcccgaa cacgggaaac
     4981 aacatcaata tattgattta caaatataaa aatcatgcta gcgacaaata gatacatttg
     5041 ttttgtcaac aatattcacg atttcccatt acaaacctcc cttacacccg cttttttccg
     5101 tcccaaaaac acaaaataaa tcaacacttt catttctccg caaaagcggt tataatcacg
     5161 ccgatttttc aactttgacg aaaaatggcc acggggccat ttttttatta tgtacatagg
     5221 gagcagcatg gatattcaaa ccatcctcga aaaaaccctg cccggcctgg gctacgaact
     5281 ggtcgatttc gaactgaccg cgcaaggaac attgcgcgtg ttcatcgaca aagaaggcgg
     5341 cattaccgtc gaagactgcg caaccgtcag caaccatttg agccgcgtct tcatggttga
     5401 agacatcgac tacaaaaacc tggaaatttc cagccccgga ctcgaccgcc ccttgaaaaa
     5461 agccgccgac ttcgtgcgct ttgccggtca gaatgccaaa atcaaaaccc gcctgccgat
     5521 agacggtcag aaaaacttta tcggtaaaat caaaggctgc gaaaacgata ccgttaccgt
     5581 atccttcgac ggcaaaaccg tacaaatcga attgggcaac atcgacaaag cccgcctgcg
     5641 ccccgaattc aaattctaaa acacaacaat attggagatg ttcaaaatga gtcgtgaaat
     5701 gttacagctg gcagaagcac tggcaagcga aaaaaacgtt gatgcggaag tcgtcttcca
     5761 agcactggaa ttcgccctgt ctaccgccgc caagaaaaag gcagaccgcg aacacatgga
     5821 tgtccgcgta caaatcaacc gcgacaccgg cgaataccaa accttccgcc gttggctgat
     5881 tgtcgccgat gaagactata cctatcccga tgtcgaaaaa accatcgagg aaatccaaga
     5941 ggaaattccc ggcaccgcca tccaaatcgg cgaatactac gaagagcagc tgcccaacga
     6001 aggcttcggc cgccaagccg cgcaaaccgc caaacaaatc atcctgcaac gcatccgcga
     6061 tgccgagcgc gagcagaatc tgaacgagtt tctcgccgtc aaagaagaca tcgtgtctgg
     6121 cacggtcaaa cgcgtcgaac gccacggcat catcgtcgaa gtcgttgccg gcaaactgga
     6181 cgcgctgatt ccgcgcgacc aaatgattcc gcgcgaaaac ttccgcagcg gcgaccgcat
     6241 ccgcgccctc ttcctgcgcg tcgaagaaat cggcaacacc ggccgcaaac aagtcatcct
     6301 gagccgtact tccggcgatt tcctcgtcaa actgtacgcc aatgaagtac ctgaaattgc
     6361 agacggcatg cttgaaatcc gcgctgtcgc ccgcgacccg ggccaacgtg ccaaagtcgc
     6421 cgtcaaagcc aacgaccagc gcatcgatcc gcaaggcacc tgtatcggcg ttcgcggttc
     6481 gcgtgtcaat gccgtcagca atgaattatc cggcgagcgc atcgacgttg tcctttggtc
     6541 gcccgagcct gcccaattcg tcatgagcgc gctctcaccc gccgaagtca gccgcatcgt
     6601 gattgacgaa gacaaacacg ccgtcgatgt catcgttgcc gaagaccagc tcgcgctcgc
     6661 catcggacgc ggcggtcaaa acgtgcgcct tgcttccgac ctgaccggct ggcagctcaa
     6721 catcatgact tccgccgagg cagacgaacg caatgcggca gaagatgccg ccatccgccg
     6781 cctgtttatg gatcacttga acgtggacga agaaaccgcc gacgtactgg ttcaggaagg
     6841 ttttgcaacc ttggaagaag tcgcctatgt tcctgccgcc gaactgcttg ccattgaagg
     6901 atttgacgaa gaaatcgtcg atatgctccg caaccgcgcc cgcgatgcca tcctgaccat
     6961 ggcgattgcc gccgaagaaa aactgggcga agtatccgac gatatgcgca acctcgaagg
     7021 catagatgcc gatatgctcc gcagccttgc cgaagcaggc attaccaccc gcgacgactt
     7081 ggcagagctt gctgtggacg aactgattga aatcaccggt gtaaacgaag aaaccgcaaa
     7141 agccgtcatc ctgaccgcac gcgaacactg gtttaccgaa gacaaataaa gggggtacag
     7201 atgagtaaca caaccgtaga acaatttgcc gccgagctga aacgccccgt cgaagacctg
     7261 ttgaaacagt tgaaagaagc cggcgtcagc aaaaacagcg gcagcgattc cctgacgctg
     7321 gacgacaaac agcttctgaa cgcctacctg accaagaaaa acggcagcaa cagcagcacc
     7381 atcagcatcc gccgcaccaa aaccgaagtc agcaccgttg acggcgtaaa agtcgaaaca
     7441 cgcaaacgcg gacgcactgt caacattcct tctgccgaag aattggcagc acaggtaaaa
     7501 gccgcccaaa cccaagccgc acctgtccgg ccggagcaga cggcagaaga cgcggcaaaa
     7561 gcccgagccg aagctgccgc acgtgcagaa gcccgtgcca aggcagaagc ggaagcggca
     7621 aaactgaaag cggcaaaagc aggcaacaaa gccaaacctg ccgcgcagaa acccaccgaa
     7681 gcaaaagccg aaaccgcacc cattgcggcg gaaaccaaac ccgccgaaga aagcaaagcg
     7741 gaaaaagccc aagccgacaa aatgccgtct aaaaaacccg ccaagcccaa agaaaaagcc
     7801 gccaagccga aacacgagcg aaacggcaaa ggcaaagatg ccaaaaaacc ggcgaaacct
     7861 gccgcacctg ccgtgccgca acccgtggtc agcgcggaag aacaggcaca acgcgatgaa
     7921 gaagcacgcc gtgccgccgc acttcgcgcc caccaggaag ccctgttgaa agagaaacag
     7981 gaacgccagg cacgccgcga agccatgaaa caacaggcag aacaacaggc aaaagccgca
     8041 caggaagcca aaaccggcag acagcgtccc gccaaatctg ccgagaaacc gcaggcagcc
     8101 gcgccggccg tcgaaaacaa gcctgtcaat ccggcaaaag cgaaaaaaga agaccgccgc
     8161 aaccgcgatg acgaaggtca aggccgcaac gccaaaggca aaggcggaaa aggcggacgc
     8221 gaccgcaaca atgcacgcaa tggcgacgac gagcgcgtac gcggcggcaa aaaaggcaaa
     8281 aaactcaaac tcgagccgaa ccaacacgcc ttccaagcac cgaccgaacc cgtcgttcat
     8341 gaagttttgg ttcccgaaac cattaccgtt gccgatttgg cgcacaaaat ggcggtcaaa
     8401 ggcgtggaag tggtcaaagc cctgatgaag atgggcatga tggttaccat caaccaatcc
     8461 atcgaccaag acaccgccct gattgtcgtc gaagaactcg gacacatcgg caaacccgcc
     8521 gcagcggacg accccgaagc cttcttggac gaaggtgcgg aagcagtgga agccgaagca
     8581 ttgccgcgtc cgcccgttgt taccgtgatg ggtcacgtcg accacggcaa aacctcgctg
     8641 ctggactaca tccgccgtac caaagtggta cagggcgaag cgggcggcat tacgcagcac
     8701 atcggcgcgt accacgttga aacccctcgc ggcgtgatta ccttcttgga cactccgggc
     8761 cacgaagcct ttaccgctat gcgcgcacgc ggtgcgaaag caaccgacat cgtgattctt
     8821 gtggtcgccg ccgacgacgg tgtgatgccg caaaccatcg aagcgattgc ccacgccaaa
     8881 gcggcaggcg taccgatggt ggttgccgtc aacaaaatcg ataaagaagc cgccaaccca
     8941 gagcgtatcc gccaagagct gacccagcac gaagtcatcc ccgacgattg gggcggcacg
     9001 gttcaattca tcgacatttc tgcgaaaaaa ggaacgaaca tcgacgcgct gctcgaagcc
     9061 gtattgctcg aagccgaagt attggaactg accgcacctg tcgatgcgcc cgccaaaggc
     9121 atcatcgttg aggcgcgctt agacaaaggg cgcggcgcgg ttgccacatt gctggttcaa
     9181 agcggtacgc tgaaaaaagg cgatatgctg ctggccggta cggcattcgg caaaatccgc
     9241 gcgatggtcg atgaaaacgg caaatccatt accgaagccg gcccgtccat cccggtcgaa
     9301 atcctcggct tgtccgacgt tccgaacgcg ggcgaagacg cgatggtatt ggcggacgag
     9361 aaaaaagcgc gcgaaatcgc cctcttccgc caaggcaaat accgcgacgt gcgccttgcc
     9421 aaacagcagg cggcgaagct ggaaaatatg ttcaacaata tgggcgaaac ccaagcccaa
     9481 tctttgtcgg tcatcatcaa ggcagacgtt cagggctctt acgaggcttt ggcgggcagc
     9541 ctgaaaaaac tgtccacaga cgaagtgaaa gtgaacgtgt tgcacagcgg cgtgggcggc
     9601 attaccgaat cggatgtcaa ccttgccatc gcttcgggcg cgttcattat cggctttaac
     9661 gtgcgtgcag atgcctcttc gcgcaaactt gccgaaaatg aaaacgtgga aatccgctac
     9721 tacaacatca tctatgatgc catcgacgac gtgaaggcgg cgatgagcgg catgctttct
     9781 ccggaagaga aagaacaggt taccggtacg gtcgaaatcc gtcaggtcat ctccgtttcc
     9841 aaagtcggca acattgcagg ctgtatggtt accgacggcg tggtcaaacg cgattcccat
     9901 gtccgcctca tccgcaacaa cgtagtcatc cacacgggcg agctgtcgtc tttgaaacgc
     9961 tacaaagacg acgtaaaaga agtccgcatg ggcttcgagt gcggtctgat gctcaaaggc
    10021 tacaacgaaa tcatggaagg cgaccaactg gaatgcttcg acatcgtcga agttgcccgc
    10081 agcctgtaat tcctttgcaa ataaaatgcc gtctgaagcg ttcagacggc aacgaaacgg
    10141 gttctgtatg ttgcagaacc cgttttttgt cgcaaatcgg cttcagacgg cattattttc
    10201 ccgacaggcg gatttgaagt ttctccacat atccggcgcg ttcggcgcgg ctggtttccg
    10261 ggcggtgcgt attgagcgac gaccatttcc aatgactgcg ggctttgttg agttctggcg
    10321 ggagtctggc ggcgtcccaa ggggctttgc ggctgtgcag ctcgatatcc gactgtgccg
    10381 cgtgtccgcg cgtttgcagg acgtggagca aaccgagggc gcgggcggcg agcagggtca
    10441 gggtttcagg gtcggtgtgc agggtttgac ggccggtgag tttgtcagaa atggtgcggg
    10501 tattgggcag gatgccgccc aaaaagccgc cgattgccgt acccaatccg agcgagccgc
    10561 cgaatgtagc gatgtccagc cccaagccga tgagcgcgcc ggttgccgcg cccgtgccga
    10621 tgcggatgcc gtattgtttg agcaattcgc tgtcgaacgg gtcttggcgg aaggcttgcg
    10681 gcatccagtc gccgccgtcg atttcgctgt ggtagaaacg gtagagggca aacagccgct
    10741 gctgcatctg ccgttcgagt tggcgtattt ccgcctgcat ggtttgcagc acggtggcgg
    10801 tatcctcgtt ttcgtccact tcctgcctga aggcggcggc atcgagcagg aaatcggcga
    10861 tttcgcggcg cgcttcgccg tccagccgct gccattcgcg ccggcgcatg gctgtcaggc
    10921 ggtcaagtgt gctgcgttcg ggcaacatgg tggcgaggtt ttcccacagg cgtagttcgc
    10981 cttcaaaatc aaaggcgacg gtgtcgaacc ctgcgaaaac gtgcaggttt ctcctcgcca
    11041 gcatggttgt ccacgattcg ggaggctgtc cgccggtaaa gttgaacacg ggcataaccg
    11101 gtttggcgca ccatgaaagg ataatcaatt catcccgata tttgtcgaga accggctcgc
    11161 gcgcgtcgat gacgtacatt gccatatcgc tttgcaagac ttgccgtaag actttggctt
    11221 cctgattgaa atcatggtgc gcgccgtggc tgccgagaaa ctgttgcagc cgttcgatgc
    11281 cgtctgaacg attgtccgta tggttttcca gccattccag cacgccgccc gcgtcttcga
    11341 gtccgggcgt gtcatacagg aaaaccagca tgtccgcgcc gtcgctgatg gcggcttctt
    11401 cgacatggcg cgtggtcgat ggggcgtttc tgacttcgcc gaaaccgctg tcgcgcaaaa
    11461 gggtacgaag gagcgaggtt ttgccggtat tggtatgccc gacgacggcg agggaaaggg
    11521 gttgtttgtt catgatgttt ttgaagaatg gattttcaga cgacctttta aggatggcgg
    11581 ctttatgggg cgtttcaagt gcggtcgttg gttttcagac ggacttcctg cgccaccctg
    11641 tcaggctcaa gccacgccgc gccgcattcg gtcagcgcgt tacgccaatg ttccagcttt
    11701 tccgcaaggt cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc gccgccctgc
    11761 gccgcttccg agagccggac aatctgccgc aacacgcccc ggtccggcac agtttgggcg
    11821 cgtacgccga taagcagttg cgccggtttc tgcttcagct ctgtctccag cgcggcaacc
    11881 tgttcccgat tggcggcaac gcccttatcc agccattcct gcgccagcct gccttcaaac
    11941 cacgtccctt cgtgccaccc ggtttccagc atcaccgccc acttgggcgc gtcgtttaag
    12001 atgattttcg gagaaacggc agacacggtt tcctgatgcg tatccgcatc ggtgattttg
    12061 ttctgccaac ggcggatgac cgcctgataa taaggtttct ctaaatccag cttactttgg
    12121 cttgttttca aaaagatttt acacactacc caagccagca ggcgcggcag gatgccgtag
    12181 caggcgatac tgccgaccag cagccccgac caagcccgcg catcggcaat attgccgttc
    12241 agacggcctt cgatgaccgc ccgcgcatca ggcacgggaa aacccagttt cgcaggcagc
    12301 catgccaaca tttccaccgc gcgtaccgaa gcggcattgc tcaacagcgt gctttcccag
    12361 ttgaacgtat attgccgcac caaaagcagc agcaataccg acaccagcat tccgagcagc
    12421 gtgcagagcc acaggctgtg cgacgttgcg cctattttcc aacgtaccga aggttgccgc
    12481 cactcgtccg catacagccg caacaccgcc tgattgacag ggtctttgcc ccgaaaccac
    12541 gtcgccggac tgctgaaaaa acgccccact ttcacgcgca ggaacaacgt tgccaaccat
    12601 actgctaaca tcagcgtatt catgcccaac acgcccgcca aaatcaggaa gaaattcagc
    12661 ccctgattgt ccatcagaag ataagtgcct gaaaatccgg cggtaaacat catcgatgct
    12721 gccaccaccc acaaccagaa cgaccccaca cgcacacgtt ccaacgtgtc ccgcagcata
    12781 cggttcctgt cgatcatctc cgcccgacgg atgatttttt cctccgtact gccgtccacg
    12841 cggcgcaaag cctccgtcgc ctgcacggga tcgccgctga aaatatagcc gccctcttcc
    12901 aaaatgcgaa ccaattcaac cagtttttgg gaaggattca acataaaatg ccatctgaaa
    12961 ataaaaaaac agattttaac acacgcattt tcaagaatat tcacagtgta ggcaaagagt
    13021 aaatcccaca cagaggcaaa agtatcggcg taaactgact gcctctactt tcccgaaaga
    13081 ttgtgcgatg tatacaggcg aacgcttcaa tacttacagc catttgagcg gtttgattct
    13141 ggcggcggca ggtttggcgc tgatgctgct gaaaaccata ggacacgggg acggctaccg
    13201 tatcttcagc gtatcgattt acggcatcag ccttcttctg ctctatttga gttcctcgct
    13261 gtaccacgga attgcagccg gaaaactgaa aagcattttg aaaaaaaccg accactgcat
    13321 gatttatgtg ctgattgccg gaagctacac accgtttgca ctggtttctt tgagaaacgg
    13381 gccgggctgg acggtatttt cactgtcctg gctgctggcg gctgcaggaa tcgcacaaga
    13441 actcaccatc ggacggaaaa gcgaaaaacg actgctgtct attgcgattt atgtcgtcat
    13501 gggttggatg gtcttggcgg taatgaaatc cctgacagcc tcactcccgc tggcaggact
    13561 ggcttggctg gcggcaggcg gtatgctgta cagcgtcggc atttactggt ttgtaaacga
    13621 tgaaaaaatc cgacacgggc acggaatctg gcatctgttc gtattgggcg gcagcatcac
    13681 ccaatttgtc agtgtgtacg gttatgtaat ctgaatgccg tctgaaaagc aaaacctccc
    13741 gatcctgaag attgggaggt tttctgtttg ccggacatca gcccttgtcg tggaactcgt
    13801 gaaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa taatcatcat
    13861 aaatggcacg gatttcctta cgcaacaaaa acagggctat caagttcggg ataaccataa
    13921 agccgttgaa catatcggac aggctccaaa ccaaatcgac tttgccgagc gtacccaaaa
    13981 cgatggcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg aaaagaaaac
    14041 ggatgttgga ctcaccgaaa taataccagc cgataatggt cgtcaaggcg aaaaaggtca
    14101 gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag gcaaactggg
    14161 taaccgccgc gccctgttcg cccgaaaggt tggcatcagt cagcaggata atcaatgccg
    14221 tagccgtaca taccaaaatc gtatcgataa acacaccgac aaatgccgcc ataccttgct
    14281 gcacaggatg cttcacatca gcggttgcat gtgcatgcgg ggtagaaccc atacctgctt
    14341 cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcacgcata ccgatacccg
    14401 cagcaccgcc caaaacggct tcaggattga aggcggcggt aaagatgtgg ttgaacatcg
    14461 gcacaatata gtcggaaaat tcaaacagga taacgacggc gcacaaaata taaacaaccg
    14521 ccataaacgg cacgacaaac tgggcaatat tggcaatacg gttcacgccg ccaatcacaa
    14581 ccatgcccgc aaggacggca agcacaatac cgactgccaa agaaggcaca tcaaatgcaa
    14641 tggtaacggc agaagcaatg gagtttgcct gtgtcgcatt accgataaag cccaatgcga
    14701 taatcaacgc aatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg ccgattttcg
    14761 gagtcagacc gtgcgtaata taaaacgccg gcccaccgat gtatttgccg tggctgacga
    14821 cgcggtattt ctgcgccagc agtgcctccg caaaaatcgt ggacatcccc aaaacggcag
    14881 aaacccacat ccaaaaaatc gcgcccggcc cgcctgcggt gatggcggtc gccacgccgg
    14941 cgacgttgcc cgtaccgatt tgcgcagata tggcaaccgc caacgcctga aactgcgata
    15001 aagacttgtc gtctttatcg cctttggcaa acaagccgcc gaatacggat ttgaatcccg
    15061 cgcccagctt ggtaatctgc ggcgcaccga gatacagcgt aaaaaacagg ccgataccca
    15121 aaagcgcgta aatcagcagg tagtcccaaa ggaaccgatt gactgtaccc accagaacag
    15181 acaatatatt ttccataaaa taaaccttat cttacaatta aaatgactgc cttccaaaag
    15241 acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat gccttaaatt
    15301 gtcaacaatc ggggagaagc tgcgccatca taccgtaaaa tatcgttaat taaaacattt
    15361 ctttattttt aagcggaaag cggaggaaat cgctttcaga cggcatcgac aacggcacgg
    15421 cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc gtaaaaaatc
    15481 tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg ccatagcaag
    15541 tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa aatcttcacc
    15601 cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgaccc cggttcaaat
    15661 ccgcgcctgc gcctggcttt ggaaaaagca gccgaaaaca atatgcccaa agacaatgtg
    15721 caacgcgcca tcgacaaagg tacgggcaac ttggaaggcg tggaatacat cgagttgcgc
    15781 tacgaaggct acggcatcgg cggcgcggct ttgatggtgg actgcctgac cgacaacaaa
    15841 acccgcaccg ttgcggacgt acgccacgcg tttaccaaaa acggcggcaa cttgggtacc
    15901 gacggctgcg tggcgttcaa cttcgttcat caaggctatt tggtgtttga accgggcgtt
    15961 gacgaagacg cgctgatgga agcggctttg gaagccggtg cggaagacgt ggttaccaac
    16021 gacgacggtt ccatcgaagt cattaccgcg ccaaacgatt gggcgggcgt aaaatccgct
    16081 ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc ccaaaacgaa
    16141 accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc gctggaagat
    16201 ttggacgacg tgcaagacgt ttacacttcc gccgtattga atctggactg atacgcagca
    16261 cagcagacat acaataaaat gccgtctgaa cctttcagac ggcattgatt tatttagccc
    16321 ttgcccacgc ccaccaaacc gtcaggacaa ccgccagaaa atacgccgcg ctccaaacag
    16381 gcaaagcaac ccctaacaga taatccggtt cggcacaatt tccgaacccg cgcacgacag
    16441 gctcgaacca atcaaacaaa ggccagccct tcaatcgaaa cgtccacggc gcgccgcacg
    16501 aaggagccgt acccggcggc agcgactgca gccacaactg atatgccgca acagaaatac
    16561 ccgtaacggc cggaatactg ataaagacag caccgaacaa accgcctgcc cttcctcttg
    16621 gtctgcacat caggacaatt gccgtacaca atgcggttgc caaaacgcat aaccgctgac
    16681 tgatacacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa gaaccggcaa
    16741 atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac ggggtcattt
    16801 tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc ccgagatggc
    16861 aagggacgaa tcgacaccgg cgcaggtgcg gacgatttcg gttttcccga tatacacaag
    16921 acctgacaag ggacaaaacg gcgatttgcc cgcaaaaaaa tacatccgga ggatttgaat
    16981 ttcggcaaat ttagcgaatc aaaagtttat ttcaatgaaa tcatatgatt tttttgaata
    17041 agcggattga caggtttttg aaggaatttg ttaccggata gccatcgggc aagttttttg
    17101 caaaatttga atcggtcggg taaattttca aaaaataatt gacagcggat aagaaacggc
    17161 ggataatttc cgccgtcgag ttgcttgatg cagcttgatt tttctcctct atttctcctt
    17221 tgtagacttg gcacacattc aactggatgt gtgcattttt ttatctccgc tttttttgaa
    17281 atttaattac ttttattgtt tgaaattcca ttctttaaga aatttaacaa gaaaaacgct
    17341 tgctttttga ccggaaaagc tgcataattc attccgttag ctggttcgag tagtcagtta
    17401 atagtttctc ctctatttct cctttgtaga cttggcacac attcaaccgg atgtgtgcat
    17461 ttttttatct gaagcaacaa gcctctgtgc gtgatgttgt tatgtttcat ttaggtgtca
    17521 aaccgcatag ccagcctgaa atattcaatc caaatccaaa accggatttt ctttgacctc
    17581 ctccatcaca acataactcc tactctccga agcggcaggc agttgcaata ggatattgcc
    17641 tagcatatcc cgataggcag acatatcggg caaacgtact ttaatcagat agtcgtattc
    17701 gcccgacacc aagtggcatt ccataatttg cggaattttc agcacttctt ttttgaaatc
    17761 ttcgaaaata ttgcccgatt tggattgcag cttcagctcg acaaaaacca atagtggttt
    17821 gcccaacaga tggggattga gatgggcgtg atagccggaa atataatgtt cccgctccaa
    17881 acggcgcacc ctctctgtaa cgggcgtggt ggacaagcct accttctcgg caagctccgt
    17941 catcgggatg cgggcattct gttggaggat cttaagaatg cggaaatcga ttttatctag
    18001 ttctttcatt tggattgcct tgtatttatt attgatttta acaaatagag tataaatttg
    18061 agaaagcgat tatatcagga aaagcaaacc gccttcctac ctgaaaactg ctgcttcggc
    18121 ttgaagatac aagggtcttt aatattttaa aagccttgcc gttggattat aattccctaa
    18181 ttgatttctt aattttgcta ataaacactt gtttggtaag gaatgaattt atgcggcctt
    18241 tgaacgtgca gattaggttg ggcaacctta ggcacaatta tcggattttg aaggaaatgc
    18301 acggaggcaa actgttggcg gtagtgaagg ccgacgcata cggacacggt gcggtcagat
    18361 gtgctttcgc gctggcagac ttggcagacg gctttgccgt ggcgacaatc gatgaaggaa
    18421 tcaggctacg ggagagcggc attacccatc cgattgtcct tttggaaggc gtatttgaag
    18481 catcagagta cgaagcggtc gaacaatact cgctttggcc ggcagtcgga aaccaatggc
    18541 agcttgaggc tttgctgatc cgccattgga aaaaacccgt caaagtctgg ttgaaaatgg
    18601 attcggggat gcaccgtacc ggttttttcc ctcatgatta cgcttcggca tatgcggcat
    18661 tgaagcagtc ggaatatgtg gacagtattg tcaaattctc gcatttctcc tgtgcggacg
    18721 aacccgaaag cggtatgacg gaaatacaga tggaagcatt cgatttgggt acggaagggc
    18781 tggaaggcga agaaagcctt gccaactccg ccgctatttt gaatgttccc gaagcacgca
    18841 gggactgggg gcgcgccggt ctggcgttat acggcatttc cccgttcgga ggaggcgatg
    18901 acaggctgaa gcccgtgatg aggctttcaa cccgtatttt cggcgaacgc gttttacagc
    18961 cgcactcccc tatcggttat ggcgcaacat tttataccag caaatctacg cgcgtcggcc
    19021 tgattgcctg cggttatgcg gacggttatc cgcgccgcgc cccaagcaat tcccccgtcg
    19081 ctgtcgacgg caaattgacc cgggtcatcg gcagggtctc tatggatatg atgaccatcg
    19141 agctggatgc ttcgcaagaa ggtttgggac acgaggtcga actgtggggc gatacggtca
    19201 acatcaatac cgttgccgaa gcggccggaa ccatccctta cgaattgatg tgcaatatca
    19261 aacgtgcaaa attcacttat atcgagtaat cgagtccaaa caaaaatgcc gtctgaagcc
    19321 tttcagacgg cataaatgcg ttttgacaaa atcagtatag tggattaaca aaaatcagga
    19381 caaggcgacg aagctgcaga cagtacaaat cgtacggcaa ggcgaggcaa cgccgtactg
    19441 gtttaaattt aatccattat aaaagacatt gccaaataga aaatactgat aatgcccaca
    19501 atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa atcagaaacc taaaatttcg
    19561 tcattcccgc gcaggcggga atccggtttt ttgagtttcc gtcatttctg ataaattctc
    19621 gtcgtttttc atttctagat tcccgcctgc gcgggaatga caaaattaaa gtttcagaat
    19681 ttatttgaac aacaaactag attcccgctt tcgcggggat gccggcggat aggttttgtt
    19741 gtttcggata aattcctgtt gttttttggt ttccagattc ccacctttaa aagaatgacg
    19801 taagaaagca gaaataagga cacagggatt ttttatagtg gattaacaaa aatcaggaca
    19861 aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg ctgtactggt
    19921 ttaaatt
//
LOCUS       DAXIEB010000028        19762 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000028 DAXIEB010000000
VERSION     DAXIEB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.028"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(209..284)
                     /locus_tag="ACFOA3_000822"
     tRNA            complement(209..284)
                     /locus_tag="ACFOA3_000822"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="ACFOA3_000823"
     CDS             complement(377..736)
                     /locus_tag="ACFOA3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6897853.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="ACFOA3_000824"
     CDS             complement(759..1403)
                     /locus_tag="ACFOA3_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6897854.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="ACFOA3_000825"
     CDS             complement(1405..1941)
                     /locus_tag="ACFOA3_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6897855.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="ACFOA3_000826"
     CDS             complement(1953..3050)
                     /locus_tag="ACFOA3_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6897856.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="ACFOA3_000827"
     CDS             complement(3053..4489)
                     /locus_tag="ACFOA3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6897857.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNMQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="ACFOA3_000828"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="ACFOA3_000828"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6897858.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPEAFIPHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSASGRQ"
     gene            complement(5479..6126)
                     /locus_tag="ACFOA3_000829"
     CDS             complement(5479..6126)
                     /locus_tag="ACFOA3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6897859.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERVLQGISLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADFIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="ACFOA3_000830"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="ACFOA3_000830"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6897860.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            7501..8577
                     /locus_tag="ACFOA3_000831"
     CDS             7501..8577
                     /locus_tag="ACFOA3_000831"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6897861.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8784..10250)
                     /locus_tag="ACFOA3_000832"
     CDS             complement(8784..10250)
                     /locus_tag="ACFOA3_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6897862.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10448..11371)
                     /gene="speB"
                     /locus_tag="ACFOA3_000833"
     CDS             complement(10448..11371)
                     /gene="speB"
                     /locus_tag="ACFOA3_000833"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6897863.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11470..13362)
                     /gene="speA"
                     /locus_tag="ACFOA3_000834"
     CDS             complement(11470..13362)
                     /gene="speA"
                     /locus_tag="ACFOA3_000834"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6897864.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQIVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13529..13813)
                     /locus_tag="ACFOA3_000835"
     CDS             complement(13529..13813)
                     /locus_tag="ACFOA3_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6897865.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="ACFOA3_000836"
     CDS             complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="ACFOA3_000836"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6897866.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFQDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15830..16531)
                     /locus_tag="ACFOA3_000837"
     CDS             complement(15830..16531)
                     /locus_tag="ACFOA3_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6897867.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16674..17822)
                     /locus_tag="ACFOA3_000838"
     CDS             complement(16674..17822)
                     /locus_tag="ACFOA3_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6897868.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17983..18246)
                     /gene="rpsT"
                     /locus_tag="ACFOA3_000839"
     CDS             complement(17983..18246)
                     /gene="rpsT"
                     /locus_tag="ACFOA3_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6897869.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18549..19691
                     /locus_tag="ACFOA3_000840"
     CDS             18549..19691
                     /locus_tag="ACFOA3_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6897870.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5087 a         5105 c         4597 g         4973 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccagaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccaag taagcagctt ctcactgctg
     1261 accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacaacggg tgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa gaccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgagact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgcatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca ggctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcgc gaaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaacct tcttccgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catctggaat gccgtccaaa taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgacgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggcgg
     4861 taacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta
     4921 ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgttttcaa gcctgttttg caatggggga
     5101 taaaagcctc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccgca gcccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgccat tagattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg
     5581 attggcaggc ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcggataaa
     5641 atctgcgcgc ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gtcaaacgga cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggcgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 agattttaaa cttattcctt gcaataccct ctctgccgcc caaacaccca ttgaccacgc
     5941 caccaagcgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 gtagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cgttccccaa cctgcaaaat acagaattaa atgttcgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatccg ttgtcatatc
     6181 tgccgttaaa gacaggcgga ttctggatgt gtttttgggt actgtcggcg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcagata ttccgctttg gcaagggcgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcac ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttgccgaa agtgccgacc agtaaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata
     6961 gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcagttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc
     7801 aaatccatac agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac
     7861 cctttggggc cggtttttgt ccgcttaggc gaccccaaac tcggtttggg catacacggg
     7921 accaatgcgc cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg
     7981 cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat
     8041 caaatggcgg ggctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac
     8101 ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca
     8161 aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc
     8221 ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg
     8281 ctggcgtgga tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag
     8341 gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg
     8401 cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa
     8461 ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggctgcttc cgagccggtt
     8521 gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata
     8581 accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt
     8641 tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca
     8701 tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat
     8761 tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg
     8821 ataacgaaaa aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct
     8881 cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg
     8941 agggtcatca taatgttaga tgcggccgcc atcatcagcg cggtcggcat cgccggggca
     9001 ttcagtgaaa cggcagcagc gaaaaatgcg ccgaacatag cggtaatatg tgcagtagta
     9061 ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg
     9121 ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt
     9181 ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc
     9241 cacgcgcttt tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg
     9301 cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata
     9361 agggcgggaa catctgccca caacagcaac aagataccga aaatgaccgc cataatgatt
     9421 tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg aacggcattg
     9481 ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag
     9541 gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta
     9601 ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata
     9661 aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc
     9721 ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc
     9781 ggatgtataa tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt
     9841 tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac
     9901 aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg
     9961 atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg
    10021 cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc
    10081 gcgcccaacg gcatggcctt gccgataatg gcggcaatca caccgataaa catggccagc
    10141 agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac caaggcgcac
    10201 aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc
    10261 gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat
    10321 atattcagat atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat
    10381 ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc
    10441 cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac
    10501 cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac
    10561 cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc
    10621 cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag tctatgtcga
    10681 aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa
    10741 cactgtcttc attgacttta ggggcggaca acacagtaaa aggcaatttt ttactgtgtt
    10801 cggtgcgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg
    10861 tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt
    10921 caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacgggaggg
    10981 taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg
    11041 cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc
    11101 cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct
    11161 cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac
    11221 gccctgaaac cgccatatca taaggcacac cggtaataac ccaatcggca tgactttcat
    11281 acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt
    11341 cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat
    11401 cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc
    11461 ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt
    11521 taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg
    11581 tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc
    11641 ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc
    11701 agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa
    11761 aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat
    11821 gccgtctccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa
    11881 cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg
    11941 atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc
    12001 ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa
    12061 caattcgccg acttcatgac agatatttaa atacagttgc tccgcccatg cacgttgcgc
    12121 caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc
    12181 gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc
    12241 ccacatactg tgcaacacac gcggtgcttc gggcgatggc gcatccagcc ggcgcggttt
    12301 gtaacgttca acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg
    12361 cccgctctcg gtaatgattg tcggatgcgg cagcccgtgt tcgagacaag cctggctgat
    12421 gccccatacg actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg
    12481 tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt
    12541 tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac
    12601 atcacggatg ttcccaagct gcgagcctaa atggaaatgc aaaagctgca ggcagtccag
    12661 cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa
    12721 ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc
    12781 gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat
    12841 ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc
    12901 gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc
    12961 ggcgtgtgcc aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg
    13021 tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac
    13081 caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat
    13141 gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg
    13201 atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acaatttgat tgtgttgcga
    13261 gggattgggg cggacgatga tttcgccaga atcgtcaaca tcataataac ctatgcccca
    13321 atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct
    13381 gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga
    13441 aaagcctgcc tcatacctga taaggaacag gcaaaatgcc gtctgaacgc ttcagacggc
    13501 attttggttc agcttgccat caaaggagtc agcgatcgag ctgctctttg atgattttca
    13561 ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca
    13621 aaacaggttt gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgct
    13681 ctaacatttt cacggctttt gtgcggtttt gttcaaaatg gggatcaccg taaatctgac
    13741 gctcggcaga gtcgccggca aatgcggcag cggcactcag agaaacaacg gcagccaata
    13801 acagtttttt cattttcagt ccttttttta tcggttgatt gaacaagatg tgtttttcaa
    13861 taccgccatt aaaacacagc aaaattaggt ttgaattaga gggaagtcaa gaagcgtaaa
    13921 tgccgtctga aaaaaacaga gccgtcaaac ggctccgttt tccttatgct tctttggttt
    13981 cagccacctt ttggcgcagg cgcaggctca attcgcgcaa ctgcttgtcg tccacgctgt
    14041 tgggtgcatt ggtcagcaga cattgtgcac gttgtgtttt cgggaaggca atcacatcgc
    14101 ggatagattc ggcgccggtc atcagggtaa ccagacggtc gagaccgaat gcaaggccgc
    14161 cgtgaggagg tgcgccgaat ttcaggttgt ccaagaggaa gccgaatttt tcttgttgct
    14221 cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgcg cggtggatac
    14281 ggatagagcc gccgccgatt tcccagccgt tcaataccat gtcgtaggca cgtgccaaac
    14341 aatttgccgg atcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat
    14401 ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aagtcgacaa
    14461 cccacaaagg tttccattcg tctgtgaaat agccgttgtc tttgccgtgt tccaaaccga
    14521 ctttgatacg caatgcgccg atggcttcgt tcacgacttt ggctttgtcc gcaccgaaga
    14581 agatgatgtc gccgttttgc gcgccggtac gcgcaatgat ttccttcagg gcgttttcag
    14641 acaggaattt cacaatcgga gattgcagac cgctgtcttc gccgttggag aggttgccca
    14701 catcgtttac tttgatgtat gccagacctt tcgcgccgta gatgccgaca aatttagtgt
    14761 attcgtcgat ttctttgcgg ctgaatttcg cgccgttcgg tacacgcaga gcgactacgc
    14821 gaccgccttt catgtcggct gcgccacgga agactttgaa ttcttccgtt ttcatcaggt
    14881 cggtcaactc ggtgaatttc aagttgatac gcatgtccgg tttgtcagag ccgtagtaga
    14941 acatggcttc agagtaaggc atgcgtggga aatcgcccaa gtccacgccc aatgcatctt
    15001 ggaagacttg tttcgccatg ccttcggtga tatccatgat ttcatcctcg tttaagaacg
    15061 aggtttccaa gtcgatttgc gtgaattcag gctgacggtc ggcacgcaag tcttcgtcgc
    15121 ggaagcactt ggtgatttgg tagtaacggt cgaaacccgc caccatcaac agttgtttga
    15181 acaattgcgg cgattgcggc agcgcgaaga actcgcccgg atgaacgcgg ctcggcacga
    15241 ggtagtcgcg cgcgccttcg ggcgtggagc gggtcagcat cggcgtttca atgtcgatga
    15301 agccttgtgc gtccaagtag cggcgaacgc ccatagcaac ttggtaacgc aggcgcaggt
    15361 tgcgttgcat caccggacgg cgcaagtcga taacgcggtt ggtcaggcga acgttttcgc
    15421 tgatgttttc atcgtcgatt tggaacggcg gcgtggcggc ggcgttcaag acttcgattt
    15481 ctttggcaag gatttcgatt ttgccggaaa tcattttatc gttggtcgtg ccttcgggac
    15541 ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgagcggaa tcggcagcgg
    15601 caaacgcttc gggcgtgtcg ggatcgatca cgacttggac gatgccttcg cggtcgcgca
    15661 ggtcgataaa aatcacaccg ccgtggtcgc gccggcggtg cacccagcct ttaacggtaa
    15721 cggtttggtc taagtattgc tcgctgatca ggccgcaata gttggtacgc ataaaatcac
    15781 cttttattga tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt
    15841 cgggcaaatc cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt
    15901 caggaatgac catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga
    15961 gttcgcgcac atcgcttttc tttaccataa tatagtaacc gccggtcgga ttcggcgtgg
    16021 tcgggacata cacggaaaga taatcgccgt ccttcggcaa tgcggcctta accgcattcg
    16081 acacctgccc tgacacgaaa gcaatcgtcc aaatacccga ttggggaaac ggcacgagta
    16141 ccggcgtttt aaacgagcgg ctgctgtcgg acagcagcga ttcggatact tttttcacac
    16201 tcgagtagat ggatttgaca accggaatcc gccccaacag gctgtcccac gcggcaagaa
    16261 tctgccggcc caatacgttg gcggcaaata atccggttac aaacaatacg gcaatggcaa
    16321 caataacgcc cagccccggg atattgaagc ccaaaacata ttgcggccgc cattgcttcg
    16381 gcagcaggtt gacgagctga tcggacgcgg aaacgatata ggaaaccacc caaaccgtta
    16441 ccgcaatcgg cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag
    16501 ctttaccgcc ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa
    16561 accgcacatt atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac
    16621 cgccggcggc ttcagacggc acggcaactt gatataccgt ctgaacacgc ggttcagatg
    16681 ccgtccaagt cgttgaacat caacccgata ccgataccgt tctgcttgtg gttgtagtcg
    16741 atcaggctct cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt gcccttaatc
    16801 ggaaacgtgt aggcggcttc aatcgcgccg tagcccgttt tggggttgta gcgcaatacg
    16861 gaatacacat tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc ataccccata
    16921 tagtcggcaa tatcgggatt gtcgttttta tcgccgctct gatcgaacgc acgcacccac
    16981 acgcgcggaa tcaccgtcaa tttgccccat tccatgcctg ccatggcgta aatcctgttc
    17041 cacgaacgcg attcgggacg gctctgtccg ttggactggt ggacaaaacc cgcaccgagc
    17101 atacgcagcc tgccgccgaa cggcaaatcc gccttcacag gctgggtcag gaaaatttca
    17161 ggtttgtaat ccgtattgcg gaacggcgcg gatttcctgc cttggttgta aatctgccaa
    17221 tcggatcttt gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa atcttcggca
    17281 attttgcttt tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg tccgaatttt
    17341 tcctgtacag tcgtaccgcg cgtcggcgaa ctcggggcat agttgggcga attgttgtac
    17401 cagagcggca taaggtacat cggattgtgt tcgcgtacgc ccaacagccc gcgcaaatcg
    17461 tttttgtcca agtcgtacat caggctcaaa ggcgtataga tgtcggcggt ttcgcccgca
    17521 ctgtcggcag gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc gcccttatcc
    17581 aggctgctgc ggacggtttc cgtcagattg agtacagctt tcgactcctg cccttcctgt
    17641 cctgccgaag acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa acgcgtaaca
    17701 ttgtccgtca aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc catcggcaac
    17761 agtcctgtca aaagaatata gcgcatattc cgtgtattca tctccgcccc cattgtcggc
    17821 atattggttt tcaaacggca ttttatagcg gattcggata aaaaaccgca ccctttccgc
    17881 catttcggga tttagccccc gtaatacaga aaaccccgag accgtcaggc ttcagggttt
    17941 tccgcttatc gcgtatcaac cgccttggcg gttttgcaaa aatcaagcca gtgcttttac
    18001 ttttgcagac agacggcttt tgtggcgtgc cgctttgttt ttgtggaaca cgcccttgtc
    18061 ggcgatgcgg tcgatgactt tgacggactc ttggtaaacc gcttgtgcgg cagctttatc
    18121 gcctgcttcg actgctttca atactttttt cactgcagtg cggaatgcgg tacgcaggct
    18181 agcgttgtgg gcgcgttgtt tgacggactg gcgggcacgt ttacgtgctt gtgcgctgtt
    18241 tgccatattg gaatctccta gaagaaaaat aaattctgta aacgcgcaat tttaaagggg
    18301 gtttacatta tatgcaagcc ttttctctct atccgcccgt cggacaccct gacagcgcaa
    18361 aaaaacggca aaatcgtgcg gacgttttcc ccctcttttg aaaacagggt aattttttag
    18421 ggaaatcagt ccaatatcac tttgagtttg cacaaattta ctttacaatg cccaaacctt
    18481 gcgccggggt gtttatgggg cgcggggttt tctttttttc caacctcttt catcaggaat
    18541 ctgcccgtat gaaaaaatcc gtattagccg ttttggccgc gctgtctctg gcagcctgcg
    18601 gcggcagcga aaaaaacgcc gtgcaacctc aagcagacgc tgcgtctgct gccaatgccg
    18661 aagccgctgc caccgatact ctgaacatct acaactggtc gaactatgtt gacgagagta
    18721 cggtcgaaga ctttaaaaaa gccaacaatc tgaagctgac ttacgacttg tacgaaaaca
    18781 atgaaacgct ggaagccaaa atgctgaccg gcaaatccgg ttacgatttg gttgtgccgg
    18841 gcatcgcctt cctgccgcgc caaatcgagg cgggcgcgta tcaaaaagtc aacaaagacc
    18901 tgattcccaa ctataaaaac atcgatcccg aactcttgaa aatgctggaa accgccgacc
    18961 cgggcaacca gtatgccgtc ccctatttct ccggcgtgaa cacgattgcg attacggcga
    19021 aaggcaaaga gcttttgggc ggcaagctgc ccgaaaacgg ctgggatttg ctgttcaaac
    19081 ccgaatacac ccacaagctg aaatcctgcg gcatcgccct gtgggacacc ccgagcgaaa
    19141 tgttcccgat tttgctgaac tacttgggca aagaccccaa aggttcgaat cctgaagatt
    19201 tgaaggcggc ggcggaagtg ttgaagtcta tccgtccgga tgtcaaacgt ttcagcccgt
    19261 ccatcatcga cgagctggcg cgcggcgaca tctgcctggc ggcaggcaac ggcggcgatt
    19321 tgaacttggc gaaagcacgt tccgaagaag tgaaaaacaa cgtcggcatc gaagtgctga
    19381 atccgaaagg catgggcttc tggattgagt cttggctgat tcccgccgat gcgaaaaacg
    19441 tcgccaatgc ccacaaatac atcaactaca cgctcgaccc cgaaatcgcg gcgaaaaacg
    19501 gcatcgccgt aaccttcgcc cccgccagca aaccggcgcg cgaaaaaatg cctgccgagc
    19561 tggtgaacac ccgttccatc ttcccgaacg agcaggatat gaaagacggt ttcgtgatgc
    19621 cgcaaatgag tacggatgcg aaaaaactgt ctgtcagcct gtggcagaaa atcaaagtcg
    19681 gcaccaactg atttgaagca ttaaaaatgc cgtccgaaca tcgttcggac ggcattttat
    19741 attggattga aatagaaata tt
//
LOCUS       DAXIEB010000029        19690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000029 DAXIEB010000000
VERSION     DAXIEB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.029"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            494..2275
                     /locus_tag="ACFOA3_000841"
     CDS             494..2275
                     /locus_tag="ACFOA3_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6897871.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLMGVDSERGGNLAMHWFADCADIDAASWLAQHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIAHLPDGVFRVKARLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKYQGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2334..3851)
                     /locus_tag="ACFOA3_000842"
     CDS             complement(2334..3851)
                     /locus_tag="ACFOA3_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6897872.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSLAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            4028..5041
                     /locus_tag="ACFOA3_000843"
     CDS             4028..5041
                     /locus_tag="ACFOA3_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC6897873.1"
                     /translation="MKYKSLSLLPLAAALAACAGGGVAEPHVPFSIPTATPLPAEVPL
                     SSDSSNIENINTAGTGSTSSGISIQQREVEVLFGNKPFIFKTPGGAQYALSSYSDPIV
                     PSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEI
                     DLFAGGFPIGKTPKPQYSYGSDAPETALSKGKITYQVWGIRVRNGQFVTSSYTPPKNS
                     GYTFNPTNTPVFSFITANFNSNKLAGKILGNSDYGPDVEIKEATINGLTFSGTATSGG
                     QTGNLDGKFFGKFDSSYSSDTSIGGKITFDGDRSLDTVFGGVSYKKELENNTDMSTTH
                     LTK"
     gene            5072..7504
                     /gene="hupB"
                     /locus_tag="ACFOA3_000844"
     CDS             5072..7504
                     /gene="hupB"
                     /locus_tag="ACFOA3_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC6897874.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTRKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKILTSNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAK
                     NFELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSP
                     VWQNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINAL
                     SPWSAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(8073..9707)
                     /gene="groL"
                     /locus_tag="ACFOA3_000845"
     CDS             complement(8073..9707)
                     /gene="groL"
                     /locus_tag="ACFOA3_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6897875.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(9800..10090)
                     /locus_tag="ACFOA3_000846"
     CDS             complement(9800..10090)
                     /locus_tag="ACFOA3_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6897876.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            10317..10970
                     /locus_tag="ACFOA3_000847"
     CDS             10317..10970
                     /locus_tag="ACFOA3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6897877.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSDFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            11183..12718
                     /locus_tag="ACFOA3_000848"
     CDS             11183..12718
                     /locus_tag="ACFOA3_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6897878.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMSGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            12715..12807
                     /locus_tag="ACFOA3_000849"
     CDS             12715..12807
                     /locus_tag="ACFOA3_000849"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6897879.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(12843..14087)
                     /gene="lysA"
                     /locus_tag="ACFOA3_000850"
     CDS             complement(12843..14087)
                     /gene="lysA"
                     /locus_tag="ACFOA3_000850"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6897880.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(14098..14268)
                     /locus_tag="ACFOA3_000851"
     CDS             complement(14098..14268)
                     /locus_tag="ACFOA3_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6897881.1"
                     /translation="MKYGVFFAAAMALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTRK"
     gene            14339..14662
                     /gene="cyaY"
                     /locus_tag="ACFOA3_000852"
     CDS             14339..14662
                     /gene="cyaY"
                     /locus_tag="ACFOA3_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6897882.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            14689..15705
                     /locus_tag="ACFOA3_000853"
     CDS             14689..15705
                     /locus_tag="ACFOA3_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6897883.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            15730..16149
                     /locus_tag="ACFOA3_000854"
     CDS             15730..16149
                     /locus_tag="ACFOA3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6897884.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPEVLQSFFEYVPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNYVLILWDDACTIAALCINDYIHAVYDFVAQ
                     AGYCRNGFPEAQGEWLKVADRVLDDELLDKILSRKPT"
     gene            16171..16677
                     /gene="luxS"
                     /locus_tag="ACFOA3_000855"
     CDS             16171..16677
                     /gene="luxS"
                     /locus_tag="ACFOA3_000855"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6897885.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            16823..19618
                     /gene="polA"
                     /locus_tag="ACFOA3_000856"
     CDS             16823..19618
                     /gene="polA"
                     /locus_tag="ACFOA3_000856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6897886.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPSEKQAEKATVPERLDYQA
                     VTTEAQFAALLDKLAQADTIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQYVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMRRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSHWLDSAGLKTKLIMQVHDELVLEVPEAELDLVKEKLPQIMAKVDEGMLK
                     VPLVAEVGVGENWEEAH"
BASE COUNT         4734 a         5534 c         4864 g         4558 t
ORIGIN      
        1 cgaagacgca gacagtacag atagtacgga accgattcac ttggtgcttc agcaccttag
       61 agaatcgttc tctttgagcc aaggcgaggc aacgctgtac tggtttttgt taatccacta
      121 tacttggagc tgatcttgct tttcgcctaa ttctacgttt tcaaacggtt gcagctggtg
      181 gtctgccata aaggtctcct tattgtattt caggttggaa atgtttcaac acacaggacg
      241 acacataaag cgtcgcccta tgtgttgccc tgatttggaa ggggttacgc ccctcccaaa
      301 taaagtctga ttctaccgcc ctaaagggcg gggtttaaac cgaaaaggaa atacgatgaa
      361 aatccgtttt cacaatttta caccctcgcc ccccactttc tctacataaa attacatttt
      421 gccgatattt gccgaatcgt ctgaaaatat gtgtaaaaag agcgtataat caaaacattt
      481 gccccgaatt gccatgccct atttcgccct gtttgacgat gccgtaagcg gtcgcgcaaa
      541 acgctatcaa aatcatgtag aaagccgttt tttccgtccc gaagaactcg atgctttgga
      601 cggcgcgctg caatcgggct ggcaaaaagg gctgcattcg gtgttgtttg cagactacgg
      661 attcggtttg ccgctgatgg gggttgattc cgaacgcggc ggcaatcttg ccatgcactg
      721 gtttgccgac tgcgccgaca tcgatgccgc aagctggctt gcccaacatt cagacggcct
      781 ccccgccggc atttccacgc cgcaatcctc cgtatccgaa gccgattacc tcgaccatat
      841 ccgccaaatc cacgaagcca tccgacgcgg cgacacctat caaatcaact acactacccg
      901 cctgcacctg caagcctacg gcaatcccgt caaactttac caacgcctgc gccagcccgt
      961 cccctatgcc gttttgtccc acctgcccga tgcacaaggg caatccgcgt ggacgctgtg
     1021 tttctcgccc gaactcttcc tcaaaatcgg ttcggacggc accatcagca ccgaaccgat
     1081 gaaaggcacc gcgccgattt tgggcgacgg acaagacgaa cgccgcgccg ccgagttgca
     1141 agcagacccg aaaaaccgcg ccgaaaacgt gatgattgtc gatttgctgc gtaacgatct
     1201 cggcaaaatc gcccaaaccg gcacagtatg cgtacccgaa ccgtttaaag tatcgcgttt
     1261 cggcagcgtt tggcagatga ccagcaccat ccaagcccaa gccttgccgc acacctcgtt
     1321 cgccgacatc ctccgcgccg ccttcccctg cggcagcatc accggcgcgc ccaaaaaaat
     1381 gagtatgcag attatcgaat cgctcgaagc cgaagcgcgc ggactttata cgggcagcat
     1441 cggctatttg aacccgtgtt ccggcggctt ggggtttgaa ggcacgttca acgtcgttat
     1501 ccgcaccttg tcgctcacgc cgctttcaga cggcatttat caaggcgtgt acggtgtcgg
     1561 ttccggcatc gtcatcgaca gcgaccctgc cgccgaatat cgggaatgcg gctggaaagc
     1621 ccgtttcctc aacgaattgc gccccgactt cggcattttt gaaaccctgc gcgtggaaaa
     1681 caggcaatgc gccctgctcg accgccacct atgccgtctg aaaacctccg cccaagccct
     1741 caacctgccc ctgcccgacg gttgcgaaaa tcaaatcaaa caatacatcg cccatttgcc
     1801 cgacggcgtg ttccgcgtca aagcccggct cgcttcagac ggcatcagcc tgtcccgcgc
     1861 cgttttaaac cgtctgaccg acaaacaacg cgtcatcatt tcgcccgccg tcctgcccgc
     1921 acaaaactac ctgcgccgct tcaaaaccac ctgccgcacc gtcttcgacc aagcgtggca
     1981 aaccgccgaa acacaaggcg cgttcgacag cctgtttttc aattcagacg gcatcctgct
     2041 cgaaggcggc agaagcaacg tgttcgtcaa ataccaagga caatggctta cgccctcttt
     2101 agatttagac attttaaacg gcataatgcg ccaagccgta ttggacgaac cgcaaaaata
     2161 tttgcaaaca aatcaagtaa tcgaaacaca catcacacaa aaaacactgc aagaggccga
     2221 agaaatccgc ctctccaacg ccctgcgcgg cgtatttgcc gccgcccttg cctgaacgcg
     2281 caaaaatgcc gtccgaacct gtttccaaag ttcggacggc attatcccac cattcaaaac
     2341 cgccaatccg ccgacacaaa cacctcgctg ttgcggcgtt tcgcatacgg cacattactt
     2401 tccgtcctgc cgaaacgata attcaacgcc ggcacgatac ctttgtacga caacttgtcg
     2461 tggctcaaag ccagcgagac attccattcg cggttgcgtt gcgcctctgt cgagaaagcc
     2521 gcaacgccct tatagttgcg gcgggcataa gacgcggaaa cccgactgtt caaaccgccc
     2581 aactgccgcc actcctgcgc ccagccggca taaacaccgt tgcgccggta ggcggcatta
     2641 ttgaccgcgc cgcccaccgt ttcacgtttc ggcacaaacc gcacaaactg ccagccgccg
     2701 aacacagttg ccgattcacc caaacgtttt gccaacgaaa cataaaaccc gtcctgcctg
     2761 ccgttattgt attccgccct atcctgttcg cggtagcgtt ggcggtaatg ttccagcgcg
     2821 accgaaaatt gccatcccgg gtttgggcgg taagtatggg acagctgcac gccgactccg
     2881 tgcgccagca tatacggcgg caggcggcgg ttgtttaccc gttttgtttt cgcatcaaag
     2941 ccgtcgctgc ccgacaactg cacctgataa aacggcaaaa tccccgccgt ctgccgtgca
     3001 tttttatact gccaacccaa atacgccctg ccgaacccgt catcataagc tgatttttta
     3061 ctgaaataat agctcgtgcc gccgatattg gaacggaaca acaaataatg attatctgcc
     3121 aacgccgtca gtttttccgc ctcgatttca taattcaacc ctgccgcccg ctccgcccgg
     3181 ctgacactgc atatctgccg gcctccgttt tgccggcaat actgcggcgc ggcattattg
     3241 gcatttctat tgaccgccgg gctgatgccg cccgaaaaac gccagcccgt cagcccctcc
     3301 gtttttttcc gaaaacgccc cacattttcc aaaaccggcg ccggcaaatc caattttgcc
     3361 gcctccgcaa aatgcctttc tgccgacttc agtcggaaat cgtcaaactc cgccgccgcc
     3421 aaatccagca aaatccgctc gtctgccgca ttttccccgt gcagttcccg ataccgcgcc
     3481 accgcctccg ccggccttcc cgctaatttc gccagcaaag cccgcgccct gccgtacaaa
     3541 accgcgtcat aatccggcag cttggcatac aaatccgcca acgaagcgat taaatccgcc
     3601 tgattgccgt tgagcgcgtc gcgcaaacta tgttccaaca ttttcgggtg cgccaacaaa
     3661 aaatccccat caaccacgcg cggggcatca ttttcaactt tccaatccga ttccgcccat
     3721 ttatccgaca ccgaccgcca cacctgcgac aaagccttgt catccaaaat tgcaggcgca
     3781 tcagccccat aggcggcaga aacacctgcc gcacaccaaa caaccaaaaa accgtatctg
     3841 aaatacaaca tatcctgtca tttacctttc cggcaaacac gccgccgaaa cacgtcaaac
     3901 catccgaaaa acaggcagaa aaccgtgaaa accggttttg catcgcccca caaaacagtt
     3961 gacatcatat aacatttaaa ctgataatca aaactattat taacacatca tcaagattga
     4021 gtatgcgatg aaatacaaat ccctgtcctt actgccgctt gccgccgccc ttgccgcctg
     4081 tgccgggggg ggggtagccg aaccgcacgt ccccttctcc attcccaccg ccacgccgtt
     4141 gcccgccgag gtaccgctat caagcgatag cagcaacatc gaaaacatca ataccgccgg
     4201 cacaggaagc acatcatccg gcatttccat ccagcagcgg gaagtagaag tacttttcgg
     4261 caacaaacct ttcatcttca aaacacccgg cggcgcgcaa tacgcccttt catcttattc
     4321 cgaccccatt gtccccagct actcctcccc cgatttcaaa attcccgacc gccacgccgg
     4381 gcagcggctt gcagacggca gccgcatctt tatctgttgc agcgactccg gggctacttc
     4441 gtatgcggaa atcaccaagc aggattatat gaaattcggg gcgtggatag gccccaacgg
     4501 cgaaatcgac ctcttcgccg gcggcttccc catcggcaaa acgccgaagc ctcaatactc
     4561 ctacggcagc gacgcacccg aaaccgcatt gtcaaagggc aaaatcactt atcaggtttg
     4621 gggcatccgc gtcagaaacg ggcaatttgt tacttcctct tatacgccgc cgaaaaacag
     4681 tggttatacg ttcaatccta ccaatacccc cgtcttttcc tttatcaccg ccaatttcaa
     4741 cagtaacaaa ctggcaggca aaatcctcgg caacagcgat tacgggccgg atgtggaaat
     4801 taaagaagca acgattaacg gtctgacctt ttccggcact gccacatccg gcgggcaaac
     4861 cggcaacttg gacggcaagt tttttggcaa gttcgacagt agttacagca gcgacaccag
     4921 tataggcggc aaaatcacct ttgacggcga ccgctccctc gataccgtat tcggcggtgt
     4981 gagttataaa aaagaacttg aaaacaacac tgatatgagt accacccatc tcacaaaata
     5041 ataattaatc cgcttttaaa aaggttttct tatgcacatc cctttcaagc ccgtattggc
     5101 tgccgccgcc atcgcccaag cgtttcccgc ctttgcggca gaccctgcgc cacagtccac
     5161 ccaaacgctg aacgaaatca ccgttaccgg cacgcgcaaa acccaaaaac tcggcgaaga
     5221 aaaaatccgc cgcaaaacct tagacaagct cttggtcaac gacgaacacg acctggtgcg
     5281 ctacgacccc ggcatttccg tcgtcgaagg cggcagggcg ggttctaacg gctttaccat
     5341 acgcggcgtg gacaaagacc gcgtcgccat caacgttgac gggctggcgc aggcggaaag
     5401 ccgctcttcc gaagccttcc aagaattgtt cggcgcgtac ggcaacttca acgccaaccg
     5461 caacacttcc gagccggaaa acttctccga agtaaccatc accaaaggcg cggactcgct
     5521 taaatccggc agcggcgcat tgggcggcgc agtcaattac caaaccaaat ccgcaagcga
     5581 ttatgtttcc gaagacaagc cctaccattt ggggataaag ggcggcagcg tcggcaaaaa
     5641 cagccaaaaa ttcagcagca tcaccgccgc cggcaggctc tttggtttgg atgccttatt
     5701 ggtttatacc cgccgcttcg gcaaagaaac caaaaaccgc tcgaccgagg gcgatatcga
     5761 aattaaaaac gacggatatg tctataaccc gaccgataca ggcggaccca gcaagtacct
     5821 gacctatgta gccacagggg ttgcgcgctc ccaacccgac ccgcaagaat gggtaaacaa
     5881 aagcaccctg ttcaagctgg gctacaactt caacgatcaa aaccgtatcg gctggatttt
     5941 tgaagactcg cgcaccgacc gttttaccaa cgagctgtct aatttgtgga cgggtacgac
     6001 cacgtctgcc gcaacgggcg actaccgcca ccgccaagac gtgagctacc gccgccgctc
     6061 cggtgtcgaa tacaaaaacg aattggaaca cggcccgtgg gacagcctca agctgcgcta
     6121 cgacaagcag cgcatcgata tgaacacttg gacttgggac atcccgaaaa attacgattt
     6181 gcgcggcatc aacggcgagg tttaccattc gttccggcat atccgccaaa acaccgcgca
     6241 atggactgcc gattttgaaa aacaactcga cttttccaaa gccgtttggg cggcgcaata
     6301 cggcttgggc ggcggcagag gggacaatgc caactcggat tacagctatt tcgtaaaact
     6361 ttacgacccc aaaatcctca cttccaacca agccaaaatc acaatgctga tcgaaaaccg
     6421 gtcgaaatat aaatttgcct attggaacaa tgcgtttcac ttgggcggca acgaccgctt
     6481 ccgcctgaat gcgggcatac gctacgacaa aaacagcagc agcgcgaaag acgatccgaa
     6541 atacaccacc gccatccggg ggcagattcc ccatttgggt tcggaacgcg cgcacgcggg
     6601 cttcagctac ggcacggggt tcgactggcg gtttaccaag catctgcact tgttggcaaa
     6661 atacagcacc ggcttccgcg caccgacttc ggacgaaacc tggctattgt tcccgcatcc
     6721 tgacttttac cttaaagcaa accccaacct gaaggcagaa accgccaaaa acttcgagct
     6781 gggacttgcc ggcagcggca aagcaggaaa ctttaaattc tccggtttcc aaacccgcta
     6841 ccgtaatttt atcgaactga cctacatggg cgtatcctca gacaagccta acagccctaa
     6901 ttatgcgccg atttccgacg gtactgcatt ggtcagctcg cccgtttggc aaaaccaaaa
     6961 ccgctcctcg gcatgggtga aaggtttgga atttaacggc acatggaacc tcgacagcat
     7021 cggtttgccg caaggcacgc acgcaggtgt caatgtcagc tacatcaaag gcaaggccaa
     7081 acaaaccaac ggacaggaaa cccctattaa cgccctttcg ccatggagtg cggtttacag
     7141 cctgggctat gacgcgcctt ccaaacgctg gggcatcaac gcctacgcca cgcgcaccgc
     7201 cgccaaaaag ccgtccgaca ccgtccacag caacgacgat ttgaacaacc cgtggcctta
     7261 tgccaaacac agcaaggcct atacgctgtt cgacctttcc gcctacctca acatcggcaa
     7321 acaggttacg ctccgcgccg ccgcgtacaa cattaccaac aagcagtact acacttggga
     7381 atctttacgc agcatccgcg aattcggcac agtcaaccgc gttgacaaca aaacccacgc
     7441 cggcatccaa cgctttacct cgccgggcag gagctacaat ttcaccatcg aagcgaagtt
     7501 ctaagctcct gatgtgccaa tgccgtctga aacccgatga tttgggtttc agacggcatt
     7561 ttttacggtc gcaccgccgc cccctttcag gcttgtacca agcccaatcc cgcaactctc
     7621 cgtcattccc acggaagtgg gaatctagaa acgcaaagct gcaagaattt atcggaaatg
     7681 actgaaactc aacgaacctg gattcccgcc tgcgcgggaa tgacgaagct atccgcacag
     7741 aaacctgcac cacgtcattc ccacgaaagt gggaatccag aacgtaaaat ctgaagaaac
     7801 cgttttatcc gatacgtttc cgcaccgaca gacctagatt cccgctttcg cgggaatgac
     7861 ggcggaaagg ttgctgtttt tccgataaat tcctgccgct cttcgttttt gggatggcgg
     7921 gaaataaaac aaaagcgcgc gtatcaaaaa ataacaatgc aaagaacggt gttggcaatt
     7981 ttaaggtttg cgccttagcc gcacttattc gcaaaaaaac cgcacggcgt tgaccgtgcg
     8041 gcttgttgtc tgaaggcttc agacggcatt gcttacatca tgccgcccat accacccatg
     8101 ccgcccatat caggcacagc cggtttgtct tcggggattt cagcgatcat gcaatcagtg
     8161 gtcagcatca agccggcgat agatgcggcg tgttgcagcg cggaacgggt tactttggcg
     8221 gggtcgagta cgcccatttc gatcatgtcg ccgtattcgc cgctgccagc gttgtaacca
     8281 tagttgcctt tgccttccaa cactttgttc accaccacgc tgggttcgcc gcctgcgttg
     8341 gcaacgattt ggcgcagcgg agactcaacg gcgcgcaaga cgatttgtac gcctgcgtct
     8401 tggtcggcat tgccggtgtg caggttttcc aaagcagcac gggcacgcaa cagggctacg
     8461 ccgccgcctg caaccacgcc ttcttcaacg gctgcgcggg tagcgtgcag cgcgtcttcc
     8521 acgcggtctt ttttctcttt catttcgact tcggtcgcgg caccgacttt gatgactgcc
     8581 acgccgcctg ccaatttagc cacgcgctct tgcagttttt ctttgtcgta atcgctggtt
     8641 gcggtttcga tttgttggcg gatttcggca acacgcgctt cgatttgggc tgcgtcgcca
     8701 aagccgtcga tgatggtggt gttttcttta ccgatttcga tgcgtttggc ttgacccaag
     8761 tcgtccaaag tcgctttttc caaagacaga ccgacttctt cggaaatcac cacgccgccg
     8821 gtcaggatgg cgatgtcttg caacatggct ttgcggcggt cgccgaagcc aggggctttc
     8881 acggcaacgg ttttcaggat gccgcggatg ttgttcacca ccaaagtcgc caaggcttcg
     8941 ccttctacgt cttcagcgat aatcaacagc ggacggctgg ctttggccac ttgttccaaa
     9001 acaggcaaca ggtcgcggat gttgctgatt tttttgtcga acaacaatac aaacggattg
     9061 tccaaagcag cgatttgttt ttcagcgtcg ttgatgaagt aaggagacag gtagccgcgg
     9121 tcgaactgca taccttcaac cacatccagc tcgttttcca aagatttgcc gtcttcaacg
     9181 gtaatcacgc cttctttgcc gactttttcc atcgcttcgg cgataatcgc gccgacttgt
     9241 tcgtcagagt tggcggaaat cgagccgact tgggcgattt ctttagaagt gtcgcaaggt
     9301 ttggcgatgt ttttcagctc gtcaaccaaa gcggcaacgg ctttgtcgat accgcgtttc
     9361 aggtcggtcg ggttcatacc ggcggtaacg tatttcatac cttcggcaac gatggattgc
     9421 gccaatacgg tggcggtagt cgtaccgtcg cctgccacgt cgttggtttt ggacgcgact
     9481 tcttttacca tttgcgcgcc catattttca aacttgtctt tcagttcgat ttctttggcg
     9541 acggttacgc cgtctttggt gatgtgcggg ccgccgaatg cgcggtcaac caccacgttg
     9601 cggcctttgg ggcccaaggt tacgcgcacg gcgtttgcca gaatgttcac gccgtttacc
     9661 attttttggc ggacttcatt gccgaactgt acgtcttttg ctgccatttc aattctccaa
     9721 aaatcattaa aactgtctga taaaaccgtt tatgccgtct gaaggcggtt tgccgtttca
     9781 gacggcatcg tgtccgtatt tatttttcaa cgatgccgaa aatatcttct tcacgcatta
     9841 ccaacagctc ttcgccgtcg gctttgacgg tttggccgct gtatttgccg aaaatgattt
     9901 tgtcgccgac tttgacatcc agcggacggc gcgcgccgtc tttgccgatt ttgcccgcgc
     9961 ccacggcgat gacttcgccc atatcgggtt tttcggcggc cgcgcccggc aaaacgatgc
    10021 ccgatgcggt tttttcttca gcttccaagc gtttgacgac aacgcggtcg tgtaaaggac
    10081 ggatggtcat atttatgctc cgataaatgt tttgaaaaca atcatctgcc cgaacggttc
    10141 ggacagattg aagtggaaac cggacagccg tcaagcagct gcccgtataa gtcggcaaaa
    10201 ttagggtgtg tgcgggtaaa ttcaagtgag gcggaaaaaa tttatttccg ccgtttttta
    10261 tagtggacta aatttaaggg gctgtactag attagcagat atgttaccct cgaaatatga
    10321 agataacgca ctgcaaatta aagaaaaaag tacagaaaga actgctccgt ttttttgtgc
    10381 tggaagttac cgcccgttct gccgccgata ttttgggtat ccatcccaat tcggcagcac
    10441 tgttctaccg taaaatccgc acggttatca accatcattt ggccttggct gccgatgagg
    10501 tttttgaggg ccctgtcgag ccggacgaaa gcgatttcgg cggacggcgt aaaggcagac
    10561 gtggtcgcgg tgcggcagga aaagtggttg tcttcggcat tctgaaacgc aacggacggg
    10621 tctataccgt tgtggtggat aatgccaagt ctgaaacgtt actccctgtc atcaagaaga
    10681 aaatcatgcc ggacagcatt gtttataccg atagtctgag cagctacgac aagttggacg
    10741 tgagcgattt cattcattac cgcatcaacc attccaagga atttgcagac cgtcagaacc
    10801 acattaacgg cattgagaac ttttggaatc aggcaaaacg cgtcttgcga aaatacaacg
    10861 gaatcgatcg taaatctttc ccgctgttct tgaaagaatg cgaatttcga tttaacttcg
    10921 gcacaccgtc tcaacagctt aaaatcctgc gggattggtg tggaatttag ggctaatcta
    10981 gtacagcccc taaaattttt cgttttcaag ccttcaccgc ttgccatcag cgttaaattt
    11041 ttttacgata agcacataga ttgtaaacaa tcggccacaa gccggtttgt tttttcagaa
    11101 gacattatcc ctgtcagacg ctatttctat atatttcgcc tataacggct tgtttttaat
    11161 aaataactca agaggtatca acgtgtctga ttccaaaacg aaagaacgcg ccacattcgg
    11221 cacgcgccgc gcgtttatga ttgccgccat cggatccgcc gtcggcttgg gcaatatttg
    11281 gcgtttcccc tatattgctt ttgaaaacgg cggcggcgcg ttcatcctgc cctatctggt
    11341 cgcgcttctg acggcgggca tcccgctgct gctgctcgat tatgccatcg gccaccgtta
    11401 ccgtggttct gcgcccttgg ctttccgccg cctcggacga tggtttgagc cggtcggctg
    11461 gtggaacgtg atgaccaata tcgtcatctg catctattac gcagtgatta tcggttgggc
    11521 ggcaagctat acctattatt cggtcaacgc tgcctggggt gcggatccgc agggtttttt
    11581 ctttaaggac ttcctgcaaa tgtcgggccc ggaagccttg ggtttggatt ttgtcggcaa
    11641 agtggccggt cctttggcgg gcgtgtgggt ttttaccgcc gccattatgg ctttgggcgt
    11701 gcaaaagggc gtggcgcgcg cctcgtcgtt ctttatgccg ctgcttttgg tgatgttttt
    11761 gattatggtc ggcatttcgc tgacgttgcc gggcgcggca aagggcttgg acgcattgtt
    11821 tacgcccgac tggtcgaaac tcgccgattc caaggtctgg gtggcggcat acgggcagat
    11881 tttcttttcg ctttccatct gcttcggcat tatggttacc tattcttctt acttgaagaa
    11941 gaaaacggat ttgggcggca cgggcttggt ggtcggtttt gccaacagca gttttgagtt
    12001 gctcgcgggc atcggtgtgt ttgccgcatt gggctttatg gcgcaggcgg gcggtaaggc
    12061 ggtcaacgag gttgcctcag gcggcatcgg tttggcgttt atcgcctttc cgaccattat
    12121 caaccaggcg ccgatgggct ggctgattgg catattgttt ttcggttcgc tggtattcgc
    12181 cggcgttacg tcgatgattt ccatccttga agtgattgtg gcggcgattc aggacaagct
    12241 gaacatcggg cgcgtcaacg ccacgctgct ggtctgcatt ccgatgggca ttgtttccac
    12301 gctgctgttc ggtacggcga cggggctgcc ggttttggac gtgatggaca aattcgtcaa
    12361 cacctacggc attgttgccg ccggctttgt ttatgttgcc gccatcatca tcagcggcag
    12421 gctgccggaa ttacgcaagc acctgaacgc tttgtcctcc atccgcatcg gcggcttgtg
    12481 gacggtctgc gtcgtggtta ccgtcgtgat gctcggctat atgctgttta aagataccag
    12541 cggcctgatg gagaaaaatt acgaaggtta tccggatggt ttcctcagta ttttcggctg
    12601 ggggatgtcg gcggcgttgg tcgtgttcgg gctgctgctg tcgttgctgc cttggaaaca
    12661 cggtcaggat ttcaacgtca aagacgaaca cgaacatgaa caaggagaag aaaaatgagt
    12721 acttccgcca ttgtgatgat gattgtctca atcgtgataa tctggggagg gctgctgctt
    12781 tccctgttaa ggctgccgaa cgagtaagcc tttagagcgt taaaaatgcc gtctgaaccg
    12841 cttcagacgg cattttgatg ttcggcttgc aggcaggcga gttcgtttgc catttgctgt
    12901 tctaaggttt cgcgtcggcg gatgagtttg tatccgccac cgttaaccaa cacttccgcc
    12961 gcacggttgc gcgcgttgta attgctcgcc atactggctc cgtatgcgcc cgcgctgcgg
    13021 ataagcagca aatcgccttc ttcgcaggcg atggtgcggt ctttgccgag gaagtcgccg
    13081 gtttcacaaa tcgggccgac gatgttggca gtcagcggcg cgatgttttt tggttctacc
    13141 gcttcgatgt gatgataggc atcatatagg gcggggcgca ttaaatcgtt catcgccgca
    13201 tcgaccatca caaagttttt ctcttcgccg tatttgacga attcgacacg cgtcaacaac
    13261 gcgcctgcgt tgccgaccaa gctgcggccg ggctcaagaa tgagtttcag acggcgtgtg
    13321 ccgatcagtt tttgaaccgc ttgggcatac gcgcccaaat caggcacttt ttcgttttgg
    13381 taaacaatgc cgacgccgcc gcctaagtcc aaatgttcca aaacaatgcc ttcggcggca
    13441 agcgcgtcaa ccaaaatcaa aatgcgttcg caggcttcga ccagcgggct taagtcggtt
    13501 agttgggagc cgatgtggca gtcgatgccg atgattttca aattgggctg ttgtgcggca
    13561 tggcggtagg cttcgagcgc gtcggcgtag gcgataccga atttgttggc tttcaaacct
    13621 gtggagatgt aaggatgggt tttcgcatcg acatcgggat tgacgcgcag ggagacgggc
    13681 gcggttttac ccaaacgcgc ggcaactttc tgaatacggt cgatttcggg gatgctttcc
    13741 atattgaagc attttacgcc ggcattcagg gcgaactcga tttccgcctc gcttttgcct
    13801 acgcctgaaa atatggtttt tgctgcatcg ccgcctgccg ccaaaacgcg tgccaactca
    13861 ccgccggaga cgatatcgaa accgctgccc aacgaggcga agtgtttgat aatgctcaga
    13921 ttgccattgg ctttgacggc atagcagaca agcgggttca aagcggcaaa cgcggtttgg
    13981 tagtgttcaa acgcctcggt cagcgcggat tggctgtaca cataaagcgg cgtgccgaat
    14041 gcttcggcaa ggcgggggta ggggacttgt tcgcaaaaca gggtcatatc ttcgttttca
    14101 ttttcgggtt tgtggggcgg attgcggttt gctttgaagc tgcaaaccgg tttgaatcac
    14161 gccgaagcgc gccttgtcgc cttctttggg caggtagaga tcgcctttgt agccgcaggc
    14221 agagagcagc agagccattg ccgctgcaaa aaatacgccg tatttcatcg gtaaacttcc
    14281 ttcataagcg cgaatgtggc aagattcggt atcttaaaca aaaaccacac aaaaagccat
    14341 gatgaccgaa agcgagttta tccgcgcgag cgaagcatta tttgaacaca tcgaagacca
    14401 aatcgacgaa aacggctggg atttcgactg ccggtttgcc ggaaacgtac tgaccatcga
    14461 agccgaaaac ggcacgcaaa tcatcgtcaa ccgccacacg cccaaccaag aattgtggat
    14521 tgccgccaaa agcggcggct accatttcgc cgagcaaaac ggcaaatggc tggcaacgcg
    14581 cgacagccgc gatttttatg acgttttaaa cgaagccctg agcgcggctt cgggcgaagc
    14641 ggtaaagatt gccgaattgt aatttgagtg ttatcacgga aagaaaaaat gaacacacgt
    14701 cccttttatt tcggacttat atttatcgcg attatcgcta tacttgctaa ctatttagga
    14761 aacactgatt tttcccatca ttatcatatc agtgctttaa ttattgctat cttgctggga
    14821 atggcaatcg gcaataccat ttatccgcaa ttttcaacac aagtggaaaa aggcgttttg
    14881 tttgccaaag gcgcgcttct tcgcactggc attgtgctgt atggtttccg cctcactttt
    14941 ggcgatattg ccgatgtagg attaaatgct gtcgttaccg atgcaatcat gctaatttca
    15001 accttctttt ttaccgcact tttaggcatt cgttatctaa aaatggataa acaattggtt
    15061 tatctcactg gggcaggttg cagcatttgc ggtgcggcag cggtgatggc ggcagagcct
    15121 gttaccaaag cagaatccca taaagtttca gtggcgattg ccgtagtggt cattttcggg
    15181 acgcttgcta tttttactta ccccttgttc tacacgtggt cacaacattt aattaacgcc
    15241 catcaattcg gtatttatgt tggttctagt gtacacgaag tggctcaagt ttatgcgatt
    15301 ggggaaaata ttgatcctat cgtggcgaat actgccgtca tttccaaaat gatccgagtg
    15361 atgatgctcg cccccttttt attaatgctt tcttggttat taacacgtag taatggagta
    15421 tcagaaaata catcacacaa aattacaatt ccttggtttg ctgtactttt tattggtgtt
    15481 gccattttta attcttttga tttattacca aaagaactcg tgaaattatt cgttgaaatc
    15541 gattctttct tattaatttc atcaatggct gcgcttggct taacgacgca agcaagcgca
    15601 atcaaaaagg caggattaaa accgtttgtt ttaggaatac taacttattt atggctagtg
    15661 gttggtggat ttttagtgaa ctatggaata tcaaaattaa tataaaattc actaaagaga
    15721 gcgtacccaa tggcacaatt accgctatat ctgacttctg aaatcaaaga ctttactgtc
    15781 ggcacgcccg aagttttaca atcatttttc gaatatgtcc cttatggcgt cgtctttgaa
    15841 gacgacggcg acacaggcta cttctatgcc gcttcgcaag acgggattct agatgccttg
    15901 cacatctaca atgttgaaga tgtatccgac aaacatatcc ccaattatgt cttgatttta
    15961 tgggatgatg cctgcaccat agccgcattg tgtatcaacg actacattca tgccgtctat
    16021 gattttgtcg cgcaggcagg atattgccgc aacggcttcc ctgaagcaca aggcgaatgg
    16081 ctgaaagtcg cagaccgcgt cttggacgat gaattgttgg acaaaatcct atcccgaaaa
    16141 cctacataac ccactcaaaa ggataacaaa atgcccctac tagacagttt caaagttgac
    16201 cacacccgta tgcacgcccc tgccgtacgc gtggcaaaaa ctatgaccac gcccaaaggc
    16261 gacaccatta ccgtgtttga cctgcgtttc tgcattccca acaaagaaat cctgcccgaa
    16321 aaaggcatcc acacgctgga gcatttgttc gccggtttta tgcgcgatca cttgaacggc
    16381 aacggcgtgg aaatcatcga catttccccg atgggctgcc gcaccggttt ttatatgagt
    16441 ttgattggta cgccttccga gcagcaggtt gccgatgcgt ggctggcttc gatgcaggat
    16501 gtggccaatg tcaaagacca aagcaaaatc cccgagttga acgaatacca atgcggcact
    16561 taccaaatgc actcgctcgc cgaagcgcaa caaatcgcgc aaaatgtgct ggcgcgcaaa
    16621 gtggcggtga ataaaaacga agagctgacg ctggacgaag ggctgctgaa cgcctaatcc
    16681 gccaaaaatg ccgtctgaac aagggtttca gacggcattt gccttttccg ttataatccg
    16741 gggttgtgcg gggtcgggtt ttaagccggc atcgtccttc cctatttttt tctgtccctt
    16801 atcggtttta agcgggtttt ttatgtccaa cagacctaca ctcctcctcg ttgacggctc
    16861 gtcctacctc taccgtgcgt atcacgcgat ggggcaaaac ctgaccgccc ccgacggcgc
    16921 gccgacgggt gcgctgtatg gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc
    16981 gcacgattat tgcgcggtgg tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt
    17041 tgaagaatac aaggcgacgc gcccgccgat gcccgacgat ttgcgcccgc aggcggaagc
    17101 actgccggat ttagtgcgcc tgacaggctg gccggtattg gtgattgggc aggtggaggc
    17161 ggacgatgtg atcggcacgc tggcgaaaca gggggcggaa catggtttgc gagtcattgt
    17221 ttcgaccggc gataaggaca tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac
    17281 gatgagcagc gaaacgctgg acattgaagg cgtgaaggca aaattcggcg tgcgccccga
    17341 ccaaatccgc gattatctcg cgctgatggg cgacaaggtg gacaacgtgc cgggcgtgga
    17401 aaaatgcggc ccgaaaacgg cggtgaaatg gctggaagcc tacggttcgc tggctggtgt
    17461 gatggaacac gcttcggaaa tcaagggcaa agtgggcgag aatctgcgtg ccgccctgcc
    17521 gcgcctgccg ctgtcgtatg acttagtaac gattaaaacc gatgtggact tgcacgccga
    17581 gctttcagac ggcatcgaaa gcctgcgccg tactacgccg aaatgggcgc agctggttgt
    17641 cgatttcaaa cgctggggct tccgcacctg gctgaaagaa gcggaatcaa acatgaatac
    17701 cggctcgacc gatgatttgt tcggcagcga cagcatcggc gagcaggcgg ctttgaatgc
    17761 ggaaatgccg tctgaaaaac aagctgaaaa agccaccgtc cccgaaaggc tggattatca
    17821 agccgttacc accgaagcgc agtttgccgc tttgttggac aaactggctc aggcggacac
    17881 aatcggcatc gatacggaaa ccacgtcatt agacgcgatg aacgccgcgc tggtcggcat
    17941 cagcattgcg ttccaagcag gggaggcggt ttacatcccc gtaggacaca gcctgaccgc
    18001 cgcgcctgaa cagcttgatt tacaatacgt attaggccgt ctgaaaccgc atttgggaaa
    18061 ccccgccctg aaaaaaatcg ggcaaaacct caaatacgac caacacgttt tcgctaacta
    18121 cggcatcgcc ctgaacggca ttgccggcga cgccatgctc gcttcctaca tcatcgagag
    18181 ccatctcgga cacggattgg acgaattgtc cggacgctgg ctcggcttgg aaaccattac
    18241 ctacgaatcg ctgtgcggca aaggcgcgaa gcaaatcggt tttgccgatg tcgccatcgg
    18301 gcaggcgacc gaatacgccg cccaagacgc cgatttcgcc ctgcgcctcg aagcgcactt
    18361 gcgcgcgcaa atggacgaaa aacagcttga aatgtatgaa aaaatggagc tgcccgtcgc
    18421 gcaggtattg tttgaaatgg aacgcaacgg cgtgcaaatc gaccgcgccg aactcgcccg
    18481 ccaaagcgcg gaactcggcg ccgagctgat gaagctcgaa caacaagcct atgccgccgc
    18541 aggccagccg ttcaacctca attcgcccaa acagctgcaa gaaatcctgt tcgacaaaat
    18601 gggcatcccc accaaaggcc tgaaaaaaac cgccaaaggc ggcatttcca ccaacgaagc
    18661 cgtgctcgaa cagctcgcgc ccgactaccc cctgcccaaa atcatcctgc aaaaccgcag
    18721 cctggcgaaa ctcaaatcca cctacaccga caaactgccc gaaatgattt cgccccggga
    18781 taaccgcgtg cataccacct acgcccaagc cgtcgccatt acaggacgct tggcaagcaa
    18841 caaccccaac ctgcaaaaca tccccatccg caccgccgaa ggccgccgtg tgcgccgcgc
    18901 ctttaccgca ccgcaaggca gcgtcatcgt ttccgccgac tattcccaaa tcgagctgcg
    18961 cattatggcg cacctctccg gcgacaaaac cctcatcgcc gcgttccaaa acggcgaaga
    19021 cgtacaccgc cgcaccgccg ccgaagtgtt cggcacggcg cccgaaaacg tctcgtccga
    19081 gcaacgccgc tacgccaaaa ccatcaactt cggcttaatt tacggtatgg ggcaatacgg
    19141 tttggcaaaa tcattgggca tcgacaacct ttccgccaaa aactttatcg accgctactt
    19201 cgcccgctac cccggcgtcg ccgaatacat gcggcgcacc aaagaacaag ccgccgccca
    19261 aggctacgtc gaaaccctgt tcggcagaag gctctatttg cccgacatcc gcaacaaaaa
    19321 cgccaacgcc cgcgcaggag ccgagcgcgc cgccatcaat gcccccatgc aaggcaccgc
    19381 gtccgacctc atcaaacgcg ccatgataga cgtgtcccat tggttggatt cagccggcct
    19441 gaaaaccaaa ttgattatgc aggtgcatga cgaattggtg ttggaagtac ccgaagccga
    19501 gctggattta gtcaaagaaa aactgccgca gataatggcg aaagtggatg aagggatgtt
    19561 gaaagtgccg ctggtggctg aggttggcgt aggggagaat tgggaagagg cacattgatt
    19621 gaaaggtgtt atatgctatc tttatttaaa taaaatttaa tttttggtat attttttcta
    19681 aatgttccta
//
LOCUS       DAXIEB010000030        19363 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000030 DAXIEB010000000
VERSION     DAXIEB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.030"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(234..2984)
                     /gene="gyrA"
                     /locus_tag="ACFOA3_000857"
     CDS             complement(234..2984)
                     /gene="gyrA"
                     /locus_tag="ACFOA3_000857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6897887.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3082..3582)
                     /gene="hscB"
                     /locus_tag="ACFOA3_000858"
     CDS             complement(3082..3582)
                     /gene="hscB"
                     /locus_tag="ACFOA3_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6897888.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3664..3885
                     /locus_tag="ACFOA3_000859"
     CDS             3664..3885
                     /locus_tag="ACFOA3_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6897889.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     VKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(3845..4165)
                     /gene="iscA"
                     /locus_tag="ACFOA3_000860"
     CDS             complement(3845..4165)
                     /gene="iscA"
                     /locus_tag="ACFOA3_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6897890.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4252..4638)
                     /gene="iscU"
                     /locus_tag="ACFOA3_000861"
     CDS             complement(4252..4638)
                     /gene="iscU"
                     /locus_tag="ACFOA3_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6897891.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4704..4832)
                     /locus_tag="ACFOA3_000862"
     CDS             complement(4704..4832)
                     /locus_tag="ACFOA3_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897892.1"
                     /translation="MNEQDLDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            complement(4899..6113)
                     /locus_tag="ACFOA3_000863"
     CDS             complement(4899..6113)
                     /locus_tag="ACFOA3_000863"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6897893.1"
                     /translation="MTIKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNISFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6142..6588)
                     /gene="iscR"
                     /locus_tag="ACFOA3_000864"
     CDS             complement(6142..6588)
                     /gene="iscR"
                     /locus_tag="ACFOA3_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6897894.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQSGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAVPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6939..8111
                     /locus_tag="ACFOA3_000865"
     CDS             6939..8111
                     /locus_tag="ACFOA3_000865"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6897895.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILVARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8247..11030)
                     /locus_tag="ACFOA3_000866"
     CDS             complement(8247..11030)
                     /locus_tag="ACFOA3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6897896.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKAN
                     AGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQEK
                     GVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFT
                     VDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFE
                     GKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLA
                     TFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQET
                     QFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKL
                     NIQNRNELQNKLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIG
                     TKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISAR
                     LGDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIF
                     IEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTE
                     SFPLGAASLEKMK"
     gene            complement(11020..12642)
                     /locus_tag="ACFOA3_000867"
                     /pseudo
     CDS             complement(11020..12642)
                     /locus_tag="ACFOA3_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003788484.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(12621..13109)
                     /locus_tag="ACFOA3_000868"
     CDS             complement(12621..13109)
                     /locus_tag="ACFOA3_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6897897.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQSVSQSVSQSVSQSVSQSVNYT
                     PN"
     gene            complement(13429..14352)
                     /gene="truB"
                     /locus_tag="ACFOA3_000869"
     CDS             complement(13429..14352)
                     /gene="truB"
                     /locus_tag="ACFOA3_000869"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6897898.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEENLPFNTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(14385..14756)
                     /gene="rbfA"
                     /locus_tag="ACFOA3_000870"
     CDS             complement(14385..14756)
                     /gene="rbfA"
                     /locus_tag="ACFOA3_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6897899.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            14947..16191
                     /gene="clpX"
                     /locus_tag="ACFOA3_000871"
     CDS             14947..16191
                     /gene="clpX"
                     /locus_tag="ACFOA3_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6897900.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(16256..17449)
                     /locus_tag="ACFOA3_000872"
     CDS             complement(16256..17449)
                     /locus_tag="ACFOA3_000872"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6897901.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            17853..18353
                     /locus_tag="ACFOA3_000873"
     CDS             17853..18353
                     /locus_tag="ACFOA3_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897902.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18438..18992
                     /locus_tag="ACFOA3_000874"
     CDS             18438..18992
                     /locus_tag="ACFOA3_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897903.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            19006..19206
                     /locus_tag="ACFOA3_000875"
     CDS             19006..19206
                     /locus_tag="ACFOA3_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897904.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT         4444 a         4565 c         4972 g         5382 t
ORIGIN      
        1 cgttcttgca gttacacctg cgacaaacag ttcaatgagt ttatttgttt ataccggctt
       61 agacgacttt ttctcatagg ggcaactcta acttaatttg aatttcccta gttatctagg
      121 acagcccctt gtttttaatt gactataatc cgctatattg tgagaagctg gatgacggat
      181 gagggataaa ataaatgccg tctgaatctt cagacggcat cgggagatga ttttcagttc
      241 tcgacttccg gttcggttac attggaaatt acagaagcgt cggagagttc ggattcgtct
      301 tcggcaacac gttccagcga taccaaggtt tcgccttcgt ccaagttaat cagtttcacg
      361 cctgctgcgg cgcggccggt ttcgcggatt tgttcgactt tggtgcggat aagtacgccg
      421 ccgctggtaa tcagcatcaa atcgtcggtt tcgccgacca aggttgcggc gaccaaatcg
      481 ccgtttcgct cgccggtgtt aatggcaata ttgccttgcc cgcctttgtt tttgcggctg
      541 taatcggcaa tcggggtgcg ttttccgtat ccgttggcgg tggcggttaa aacttgcaaa
      601 ccgctttctt cggtttcagg ggcgaaggta atcaggctga cgattttgcc gtcggcaggc
      661 aggcgcatac cgcgcaaacc gccgctgccg cgaccggacg ggcgaacacc gtgtttgccg
      721 ctcggcagtg cgttttcgct gtcggcggtt tcatcttcga tgccgtctga aatttcggtt
      781 tcgatgtcgg catcttccgc ttcgtcgttg ccggattttt cccagtattc gttgaagcgg
      841 atggctttac ctaagttgga gaacagcatg atgtcgtccg caccgcctgt ttgcgcagcg
      901 ccgacgaggt agtcgccttc tttgagcgcg atggctttaa tgccttgggc gcggacgttt
      961 ttaaaggcgg aaagttggac ttttttcacc attccctgcg cggtggcgaa gaagacgtat
     1021 tggtcttcgg ggaactcgcg tactgccaga atcgcgctga ctttttcgcc ttcttccaac
     1081 tggatgacgt tgttaatcgg acggccgcgg ctgttgcgtc cgccttcggg cagtttgtaa
     1141 accttaatcc aatgacactt gcccaaattg gtaaagcaca tcaaataatc atgcgtgttg
     1201 gcaacaaaca gggtttcgat aaagtcttcg tctttggtgg cagccgcctg tttgccgcgc
     1261 ccgccgcgac gctgcgcctg atagtcggtg gtcggctggg ttttgatata gccgccatgt
     1321 gtcagggtaa cgaccatttc gcgttgcgga atcaggtctt catcggcaat gtcgccgccg
     1381 aacgggttga tttcgctgcg gcgttcgtcg ccatagttgg ttttgatttc ttccagttcg
     1441 tcgcggatga tttgggtaat gcgttcgggt ttggagagga tatccacaaa gtcgatgatt
     1501 ttacccatca ggtttttgta gctttcgaca atttcttctt gatcgaggcc ggtcaggttt
     1561 cgcaggctca tgcgtaaaat agcatctgcc tgaatctcgc tcagataata accttgctct
     1621 ttcaagccga tgtttgcagc caatccttcc ggacgcatca tttccaaatc cagaccggaa
     1681 cgcgtcagca tttcttcaac gaggctgctg cgccaagggt gcgcaagcag tttgtctttg
     1741 gcctcggctg cgttgggcga ttctttgatg agcttgatga tttcatcgat attggacagt
     1801 gcgacggctt tgccttcggc aatatgccct tcatggcgtg ccttcttcag ccggaaaagc
     1861 gtacgtcggg taacgacttc gcggcggtgg cgcaggaatt cggagagaat ctgtttcagg
     1921 ttcaacaggc gcggttgtcc gtcgaccaaa accaccatat tgatgccgaa actgtcttgc
     1981 agcggagtca gtttgtagag ttggtttaag acgacttcgg cattttcgtt gcgtttcagc
     2041 tcgataacga cgcgcatacc ggatttgtcg gattcgtcgc ggagctcgga aatgccttcc
     2101 agtgtttttt cccgaaccaa atcgccgatt ttctcgacca gcttggcttt gttgacctga
     2161 taggggattt cgtcgataac gatggcttcg cgttcgccgt ttctgcctat gggttcgata
     2221 tgggtcttac cgcgcataac gacgcggccg cggcctgttt tatagccttc gcgcacgccg
     2281 ctcaagccgt agatggttgc cccggtcggg aagtcggggg cttggataat gtcgatcagt
     2341 tcgtcgattt cggtgtcggg tgcatcgagc aggcgcaggc aggcattgac ggtatcggaa
     2401 aggttgtgcg gcgggatatt ggtcgccatg ccgacggcga tgccggacga gccgttgacg
     2461 agcagtgtgg ggaaacgggt cggcagtaca agcggctcgt gttcgctacc gtcgtagttc
     2521 gggccgaaat tgacggtttc ttcctcaatg tctgccagca tttcgtggga aattttcgcc
     2581 atgcggattt cggtgtagcg catggctgcg gcggcaagcc cgtccaccga tccgaagttg
     2641 ccctgtccgt ctatcagcac ataacgcata gcgaaatttt gcgccatacg gacgatggtg
     2701 tcgtatacgg cggtatcgcc gtgggggtgg tatttaccga tgacgtcgcc gacaatgcgc
     2761 gccgattttt tgtaggcggc attccagttg tttttcaatt cgtgcatcgc gtacagtacg
     2821 cggcggtgta ccggcttgag accgtcgcga acgtccggca gcgcgcgccc gacaatgacg
     2881 ctcatggcgt agtcgagata gcttttgcgc atttcgtctt caaggcttac cggcagggtt
     2941 tcgagggcga atttgtggtc gtggcggatg gttgcgtcgg tcatggtttc aatgtttcgt
     3001 atggcaaaaa attgttgctt attttagcat attttgacgc ggaacggtgc ggcggttacg
     3061 ccgtctgaaa cacggtgcgg attataatgc cgaggaaatt tcgttgcgga gtttgtcgag
     3121 aaacctgcct tggcggactt gttgtgcggc tgtgtcgtaa tcttgtcggg caaacgactg
     3181 tttcagaccg cagaacagtt tttcttgttc gtcgcggatt tcgttgtcga gatttttcaa
     3241 ggattcaagg tcgttgcctg cccgtgcctc catcagcgtt tcgcgccatt ccatttgctg
     3301 cataaggaat tcgggggcga aagcggtatg ctccggcgcg tcggcatcga tgcccgatgt
     3361 tttcagcagg taggcggcgc ggtcgatggg gtttttcaag gtgcggtagg catcgttgat
     3421 ggtggaagac atcatcactg cctgcttttg ctcaaaggcg gaagctgaag cgaatttatc
     3481 gggatggaaa cgggcggcca aggcgcggta ggtttgttcc aagttttcgg tgtcgatatc
     3541 gaaagcgggt tcaatccgga agagggtgaa atattgggac atagtaggat gataaatgta
     3601 agattttggc agaaaactgt ttttgcctta taatctgcag cttcttaaac gaaaggactg
     3661 aatatgggcg gcaaagtgca gcacaataaa ggcaaaatac gcgacaatgc tttaaaagcc
     3721 ttagtgaaat ccgatttgtt ccggcacaag gtggaacgga aaaggaaagg caaaggcagc
     3781 tacaacaggc aggaagtgaa aaaatggcgg gacggttttg atacggtccc gccattttta
     3841 tgtcttaaac atggaagctt tcgccgcagc cgcaggagtc tttgacattg gggttttcaa
     3901 atttgaaacc ttcctgcaaa ccttctttgg tgtaatcgac ttgtgtgccg tccagataaa
     3961 ccaggctttt cgggtcgata taaatgcgtg cgccgtgtcc ttcgaaaatc aggtcgtcgc
     4021 catcggcttc atcgacaaat tcaaggttgt acgccatccc cgagcagccg ctggttttca
     4081 cacccaagcg tacgcccaag cctttgccgc gtttggcgag atagtcattg atgtgttttg
     4141 cggcattctc ggtaagggta atcatatttc ttccttgttg taccgcctcg gacgaacccg
     4201 aagcggcggg cggttcggac ggcattgcgg gacgatgccg tctgaagggc tttatctgtt
     4261 ttcctgacgt ttgcggtagt cggcaacggc cgcttttacc gcgtcttccg ctaagatgga
     4321 gcagtggatt tttaccggcg gcagttccaa ctcctcggcg atttcgctgt ttttgattgc
     4381 cagcgcgtca tccaggcttt tgcctttgac ccactcggta atcaggctgg acgaagcgat
     4441 ggcggaaccg cagccgtaag ttttgaattt cgcatcttcg atgatgcctt catcgttcac
     4501 tttgatttgc aggcgcatca cgtcgccgca ggccggtgcg ccgaccatgc cggtgccgac
     4561 ggactcgtcg tttttgtcga aagtgccgac gttgcgggga ttttcgtagt ggtcgattac
     4621 tttatcgctg tatgccatga tgtggtttcc ttaatgtttt gatgatttaa gtggtttgtt
     4681 tgccggtttt cagacggctt gagttagatt ttgcacgcgc cgccttcaca gccgtcgtcg
     4741 ttttccgaat cgacgccgcc ttcgacggta acgccgtcaa aatcgtcaag caggttgtcg
     4801 aggttgtcca aatctaaatc ttgttcgttc atatttttct cttgattgct ttgtttttgg
     4861 aatgccgtct aaaggttcgg acggcatgtt ggtacgcttt aatgcgccgc ccactcaatc
     4921 gaattcaaat caatcccgtc tttgaacatt tcccacagcg gcgacagttc gcgcagtttg
     4981 ccgattttgg atttaatcag ttcggcggcg aattgcactt cttcttcggt ggtcatgcgg
     5041 ccgaaggtga tgcgcaggga tgagtgcgcc agttcgtcgt tgcggccgag cgcgcgcagg
     5101 acgtagctgg gttcgagcga ggcggaggtg caggcggagc cgctggatac ggcgagttct
     5161 ttgaccgcca taatcaggct ttcgccttcg acgaagttga agctgatgtt caggttgttc
     5221 ggggcgcggt gttcgaggtc gccgttgaca tagacttctt cgatgccttc gatgcctttg
     5281 aggaagatgt cgcgcagttt gcggtagtgc gccatatctt gttcgagttc ttctttggcg
     5341 atgcggaagg cttcgcccat accgacgatt tggtgggtcg gcaatgtgcc gctgcggaag
     5401 ccgcgttcgt ggccgccgcc gtgcatttgg gcttcgaggc ggacgcgcgg tttgcggcgg
     5461 acgtacagcg cgccgatgcc tttggggccg tacactttgt gcgcggacat cgagagcagg
     5521 tcgattttgg cggcttcgac atcgacaggt actttgccgc aggcttgggc ggcatcgacg
     5581 tggaagacga ttttgcgttc gcggcagatt tcgccgatgg cgggaatgtc ttgcactacg
     5641 ccgatttcgt tgtttgccca cattacggaa atcaggatgg tgtcgtcgcg gatggcggct
     5701 ttgagttctt ccaaatcaat caaaccgttt tcctgcacgc cgaggtaggt tacttcaaaa
     5761 ccttggcgtt cgagttcgcg catcgtgtcg agtacggctt tgtgttcggt tttgacggtg
     5821 atgaggtgtt tgcctttggt tttgtagaag tttgccgcac ctttgatggc gaggttgtcg
     5881 gactcggtcg cgccgctggt gaagacgatt tctttggggt cggcattaat cagggcggcg
     5941 atgtcggcgc gggctttttc gacggcctct tctgcttccc agccgaatgc gtggctgttg
     6001 gaggcggggt tgccgaaggt ttcggtcaga tagggaatca ttttttcggc aacgcgtttg
     6061 tcaacgggtg tggtggcggc gtagtcgagg taaacggggg ttttgatggt catggtttgc
     6121 tctttctttt tcgggtgtta tttaatggat gtgtgtaaat tggacgacgc ggctgccgtc
     6181 gccgttattt ttctgttcga tgatgctttg cagggtaacg ctgccgaggt agtcgttgat
     6241 ggttttgttt aaattctccc aaaggtcgtg cgtcaggcag ggcgcgccgt ggtggcagtt
     6301 ggctttgctg ccgcattggg tcgcgtccag ccggtcttcg gcggcggaaa tgatttgggc
     6361 gatgttgatt tgtgcgggag ggacagcgag gatgtagccg ccgccgggcc cgcgcaggct
     6421 ttcaaccagt ccggcacggc ggagtttgcc gaacaattgc tcgagatagg agagggatat
     6481 gttttggcgt tcgctgatgg cactgagttt gacggcgccg ctttgcgcgt tcatcgccaa
     6541 atccagcata gcggtaacgg cgaaacgccc tttggtggtc agtctcatgg tggtggttgc
     6601 ccatgtcggt ttttttatta gtgggtggaa ttgtccaata tctgagtgtt tcagtcaagt
     6661 aagggggagc tgttgccgat tgtttgttta aatggcggta tagtctgatt ttgctttatt
     6721 tgtttggttt tttaaatcag ttggttatgt tttttgagga gcggtggtcg gggtattttg
     6781 ccggcaagcg gtttcaaaac gatttgattt atttttgata ttgatataga ttatctaaat
     6841 cttagtgatt tatttgggta ttgcctgaaa tgctgtctga aggctcaagc ggtttcggac
     6901 ggcatattcc atcaggctgt caaacaggga aaaacaagat gaaacgtgat ttgagcaaaa
     6961 tgacctgcat cgaagacttg cgccgcgttg ccaaacgcaa gatgccgcgt atgttttacg
     7021 attacatcga ttcgggttcg tggacggaaa ctacctaccg cgaaaacact tcggatttta
     7081 aagatatccg cttccgacaa aaggtattgg tcaatatgga aggtcgaagc ctggaaacca
     7141 aaatgatcgg gcaggatgtg aaaatgccgg tggcgattgc cccgactggt tttaccggca
     7201 tggcacacgc cgacggtgaa atcttggtgg cgcgggcggc ggagaagttc ggcattccgt
     7261 ttacgttgtc caccatgtcc atctgctcga ttgaagacgt tgccgagaac acttccgcac
     7321 cattttggtt tcagctttat gtgatgcgcg accgcgagtt tatggaaaac ctgattaagc
     7381 gcgcgaagga tgccaaatgt tcggcattgg tattgaccgc cgatttgcag gttttgggtc
     7441 aacgccacaa agacatcaaa aacggcctgt ccgcgccgcc gaaaccgacc atcgccaatt
     7501 taatcaatct ggcgaccaaa ccggaatggt gcatgaaaat gctgaacacg gaacgccgca
     7561 cgttccgcaa tatcgtcgga cacgcgaaga acgtcggcga tttgtcttcg ctgtcttcat
     7621 ggacttccga acaattcgac ccgcgcctga gctgggacga tgtggcgcgc attaaagatt
     7681 tgtggggcgg caagctgatt atcaaaggca ttatggaacc cgaagacgcg gaaaaagcag
     7741 cgaaaagcgg cgcggacgca ttggtcgttt ccaaccacgg cggccgacag ctcgacgata
     7801 ccgtgtctgc catcaaagcc ttgcccgaca tcgtcagcgc agtgggcagc gacatcgaag
     7861 tttggatgga cagcggcatc cgcagcggtc aggatatttt gaaggcgtgg gctttgggcg
     7921 cgaaaggtac aatgataggt cgggcgtttc tgtatggctt gggcgcgtat ggagaagaag
     7981 gcgtaacccg cgcgctggaa atcctatata aggaaatgga tatatctatg gcgtttacgg
     8041 ggcaccgcga tattcaagat gtcgatgcct cgattttaag gggtaaagat tggggcaggg
     8101 aaacggttta gatactgaaa aatacgatgc cgtctgaaca cagggttcgg acggcattaa
     8161 aatttggctg tattttaata atgaactatc tgacaaaacg cccttttgta aaggaagttt
     8221 tgtcaaacgg ggttgcctgc aatgcactac ttcatttttt caagcgatgc cgcacctaaa
     8281 gggaatgact ccgtaaattg tccatttttc tgatgatcag taaaaaacgg taaaccgatc
     8341 aaacgataat gcggtgtatc tttttgcagg atcttatcca tcccatattc cacagtgatg
     8401 gcttctaaaa attcttcttt ccatccatca gtttccacta aatgctcacc tttgggctca
     8461 atgaaaattt ggtaatgcaa aaagtcgttc acaccattgg aagaagattt ttgtttatct
     8521 ttcagcaata aaataaagtc cggcataaat ccttcgccat cggcaaagtt attcaattta
     8581 aacacttctt cattacggat taaatgaacg tcatattgag atttcaaatc gcccaaacgc
     8641 gctgaaataa attgaattaa cgcttcttcc aaacccgttc cggcaaaatt attcatcaca
     8701 taccaatcat tttgcgtggc aatagtcgtt tttacatcat cttttttgac ccatttttgt
     8761 tttggcgtgc cgaaaatttc ccataatttt ttaggtgtaa attcttttgt accgataaac
     8821 ggcatatcac tttcattcag atgtttttca accatttcca agattttcag gcagcctgca
     8881 agtttatcgt ccaggcgaac ctgtttgtct tgccctaatc ctaaaaattc aatttgccag
     8941 tctttcaaca atttattttg caattcattg cgattttgaa tattgagttt gctttgcagg
     9001 cggtcaaaat ggaataaaga ttgactattt ttccctttga tatgcagggc tttattgaaa
     9061 atgtgccgct ccatttcact catttttatg gtttgagtaa aattattttg cgtgccaagt
     9121 tgcttgactt tttcatcatt ttcatcggct gtaaattgcg tttcctgcaa cagttgattg
     9181 ccgtgaactt ggaatgaaag cgtttgcgga ttggcttgca ggctgtttgc attattggtt
     9241 ttggcgtttg ggttggggat ttttttattc gcccaaatta acaattctct aaaatcctta
     9301 ttagcggcaa attcagattt gagtttaaat gttgccaata ccttatcatc gtctttttca
     9361 ggcaaataac cgtcttttcg caactcgttt ttcagttctg aaatatagcg ggattgctca
     9421 tcgtgcgtgt aataaaacaa ttcttccaag atacgcaatt cgtgttgcat atcgttgtca
     9481 aatttgcgtt tattcggctg tttaccttca aacgcaaatg gaaaataacg cacgccacga
     9541 ccaatcaact gcttttccga tacggtggcg gcagccgttt tgcctgattt tttatttgaa
     9601 ccgccgccgt tttgcccttc atacaaacgc acaatatcaa acaaattcag aacgtcccaa
     9661 ccttcggtta atctgtccac cgtaaaaatg gcacgaatcg gattatcagc cgcttccaaa
     9721 ttattcagca atttttctgt ttcgctgtcg gttttttcgg ttttggtttt gttggtttcg
     9781 gagttggtaa taatcacatt gtgtttttga taattttgtt ttacccaatc tgccaaatgt
     9841 gcaaactcaa cgcctttttc ctgcataaat tttagggctt gttcagtgcg ggttttgcct
     9901 tgttcgttgg cgttatcgct atcgctatcg ttcaagcttg ttgaaaatgt agttaaaaac
     9961 gaaaaatcaa ccgcctgcac attttctgcc caatttaaaa atgccagata atccgctttt
    10021 gattcatcaa tcgtcttact tctaaacaac atcacaggct tgaaattggc aatgccatat
    10081 ttcaaagcga tttgatggcg ataccaagca aataataaag cgtgcaacac ccgctctttc
    10141 ttgcccagcg tactggatac caaattgatt tctttggtat aacctttttg caaaaattct
    10201 tttaagccaa attttgtgat gattttatcc gcgtattttt gttgcacgtc agcattttca
    10261 ggcagcgtgg cggtaaattc caacagcaca ttttggctgg gattgccatt tttattgagt
    10321 aacaattcca aaaccatatg ctcccagcct ttgcgttcaa tttcggcatt gcctgcattg
    10381 gcttttaatt ccacttccaa atccaactcg ccttgttttt tgcctttggt ttgcgcgttt
    10441 aaatggtgtg cttcatcgcc cagcatcaca agattcaatt cgtgcaaatc cgccaatgtg
    10501 gtttggtttt cccgttcggt gtgaatatcg ttatacagct tttgaatgct ggtaaattta
    10561 atttcaatgc cgtctgaatg tgggctgaac gtttccactt tgcgaatggg aattaccgta
    10621 tcgccctgca aaatcttctc ggtaaataaa aattttgcgt gtgtcgggtc aataaaatta
    10681 ttttccgttt tatccacgat attgttttga ttcacaaaaa acagaaaatg ccgataccct
    10741 ttttcaaaat aatacaaaat caatgccgcc atcatcatcg ttttgcctgc acctgttgcc
    10801 atattgaaca gcaaatgcgt tggcttattt tttaaatcag ggaaatcgtc tagctttgaa
    10861 gtgcggtcaa aaatcaaaaa gttttcaagg gcagattttt gccattcaaa aaaatcatat
    10921 tttaagtttt tggacgtaaa ttctggcatt tcaagtaatt caggcttgta ttttttaacc
    10981 gctttacgtt cattttcaat cacttcaaac aacgttttat tattcgccat cttttttctc
    11041 cgcttgattt tttactgatt gatagaatgc acggcttaag gctttatcat catcgttcag
    11101 gcaatctgca aattgttcgt catccatttc ggataatgaa acatacattt gatttaaatc
    11161 caacatttca agcaccattt gtttttgttc gtctaatggc aaagattgaa attcaggctc
    11221 ttcaataatt tggctaaatt catttatgct gacgttgtat ttcaagaaat agcgttcgca
    11281 taaaccttca aacagcgttt tgatgccgtc tgaatcttcg caagccaaaa tctgttgttt
    11341 tgcggtttcg ttaaatggag caagttcggc ataaacaaat tcgccaccac cttgccaatt
    11401 cacggctttg gaaataccgc cttgctcgcc atcaatcact tttttcaagc gttcaacggc
    11461 aagcgtttca atataatcca tttgttcaat accgatatat tggcggttca ttttgtgggc
    11521 aacggcggcg gttgtgccac tgcctaaatg gtagtctagg acgatgtcgt tttcggatgt
    11581 ggcaatttga ataagatact gcaataagct ctcaggtttt ggagtagtaa aaactttgct
    11641 gccgaacagt tttttcaatt catctgtcgc atgtgtattt aagatatcct gtattaagtt
    11701 cttataggga gcagaacgtt ctatcggctt gttttcgtta tcaaccaaca tatagttttt
    11761 ataacataca ctccatccag attttttaga ctttgatttt ctaaactcca aaaaaccgtt
    11821 tgttattgcc caatcaattt tatttttact ccatttccat atccagccat cattgacaaa
    11881 ttcagtcctg ccattcggat acgtaaaagt gccatctgga cattggattg caaaattcaa
    11941 actgtcacta tactgcaatc cccctctatc taaattgtcg atataatatt tgcctctttc
    12001 ctgttcaaac ttatcactta atttgtatct ctctgtatca taagaaaacg tgtttttatt
    12061 taatttaact gttttaaagt tctttgagta acataagaca aactctgtaa tagtcgctat
    12121 ctgtttggca tcggacgcac ctgtcttttt ttcccaaata aaattgcaga tgaaattttc
    12181 atttccgaaa acttcatcca ttaaaatttt caaatatgcc tgttcgttgt cgtcaattga
    12241 cacaaaaatc gaaccgtctt ccataagcag ctctttggcg atttctagac ggtttttcat
    12301 aaaagtcagc caagtggaat gattaaattt gtcgttgtat ttgaaaccgt cattgcccgt
    12361 gttatatggc ggatcaatat aaatcaactt cactttgcct ttaaactgtt tggcaagcga
    12421 atgcagggca atcagattat tgcctttgat aataagattt tcggccggtg tgccgtctga
    12481 atggcgtttg atttcgccaa ccgcttgttt gccgtctgcg gtatagcgag agaattttga
    12541 gaatgctttt gcgtcaaaaa gccgatcgat ttcatcaaaa gcaagtgttt gattaaaaaa
    12601 gatttcttgt ctttttcggg ttaatttggt gtataattga ctgactgact gactgactga
    12661 ctgactgact gactgactga ctgactgact gactgactat tattgcgttt aaaataaatt
    12721 tcttcgcctt cttcggtgct ttgtccgcca tttaacacac agtctttaaa cggaaaatcc
    12781 aacacaatat cactgctgtc tttcaaaaag cggttgccgt ccgtcaaacc aatgcggttg
    12841 gcgtattttg tgtaggaatt attgatgctg tgtttatcca agaaaaaacg aaaatcctgc
    12901 aatttaaaca ccaaaacacc attcacttcc acaaagaaat ggcgttttaa ctcatcatta
    12961 ttcaataaca aaccgataat agtcgggtcg gttttttcca ccaaatccaa cagaatgttt
    13021 ttggctaaaa tggttttttc ttcgttcgcc catacttttt cgtgtggtag cagggcttgg
    13081 gttaattcgg tttgaatgtc tgtcttcatc gttataaatc ctcatcagtt attttaatgt
    13141 gccattcttg tgtaacggca aatggctttg caaatagatt ctaataaaat caattatttg
    13201 atgtctttgc tgtttccggg ggcgttcgca aagttgcgat aagcggggcg ggtgtccgag
    13261 tgtgtcgtga ttttgcggaa atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    13321 cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc
    13381 actatatcaa cacattattc aaacacagcc cgtattttta accgccgttc aggcggcgga
    13441 tgccgccgtg ttcatcaggc gcaaggcttt tagacggcat atttcctttt gatattccgc
    13501 caggccgaca aagcggccgt tttccgtgta aacgcgtacc ggcgtgttga aaggcaggtt
    13561 ttcctcgaaa cgcggtcgtt gcccgcattg gagcatatgg acggcataat cgtttaaaac
    13621 ggtttgggga aaatgggcaa ccaatacgtc gcagggcagc agcaggctgt cgcgttctgt
    13681 ttcgtccaag tttgccaagg cttcgagcgt gtggctttgg gcgatggtaa agccggcggt
    13741 ttcggtgcgg cgcagggcgg tcaggtgggc gaacgtgccg atgtgtttgg cgatgtcttc
    13801 gctgagggtg cggatatagg tgcctttgct gcaacgcacg tcgatgacgg ctttgggtga
    13861 gtcaaactcg gcaatatcga tggcgtaaac ggtaatgtcg cgcgccttgc gttcgatgac
    13921 gatgcctttg cgggcgtatt cgtacagcgg tttgccttcg tgcttgaggg cggaaaacat
    13981 cggcggcact tggcggatgt tgcctgtcag ggcttggcag gccgtctgaa attcggctaa
    14041 ggaaatatcg gcgcgtgcgg cagcgatgat ttcgccttcg gcatcacccg tgctgctggc
    14101 ttcgccgagt ttcagcgtgg cggtgtaggc tttgtcggca tccagcaggt attgggcgaa
    14161 cttggtcgct tcgccgaagc agacgggcaa aagcccggtt gccaaagggt cgagcacgcc
    14221 ggtatgtccg gctttttcgg cacggaacaa acgccgcgct ttttgcagcg cggtgttgct
    14281 ggaaaggcct tcgggtttgt ccaagagcaa aacaccgttg acggcacgtt tttggggttt
    14341 gcctgtattc atggttcgat gttcggacgg catgggcata tccgtcagtc ttcaaccggt
    14401 ttttccgccg ctacttggtc gataagggcg gacaggttca aaccgcgttc caaggattcg
    14461 tcgtatttga aatgcagttc ggggattttg aacagcttga tgcgtttggc caattcgctg
    14521 cggaggtgtc cgcgcgcgtg ttccagcact tcttccgtaa tttcgcgcgc gtcttggttt
    14581 aaaatggtgt agaacacggt ggcgtggctg taatcgcggg taacttcgac ttcgttgacg
    14641 gtaatgaagc cggcgcgcgg gtctttcagt ccggtacgga cgagttcggc aagctcgcgc
    14701 ataatttgtt ctttgacacg gtcttggcgg gcatagccgc gttggggttt tctcatgaat
    14761 atcctcagat ttcagatttg gtttcagacg gcatttttgc cgccggggcg gttgcgaaag
    14821 ggcgtattat agcctaatcg gcgggatttt aaggtaatac attgataaga acggatatag
    14881 ttattatact tggcaacccg aacggcacgt gccgttttct gtaacgcata cattgtaaag
    14941 aaagccatgt ccaacgaaaa ccgtacctgt tccttttgcg gaaaatccaa atcacacgtc
    15001 aaacatttaa ttgagggcga aaacgccttt atctgtgacg aatgcgtctc aaactgtatt
    15061 gaaatattgc atgaagacgg aaatgacggc acgccttcgg aaagtgccgg aggggagccg
    15121 gaagaatccg gcaagctgcc cacgcccgcc gaaatcgtcg ccaacctcaa cgaccacgtt
    15181 atcggtcagg aacaagccaa gaaagccttg gcggtgtcgg tgtacaacca ttacaaacgc
    15241 ctgcgccacc cgaaagccgg agctaatgtc gaattgtcga aatccaacat cctgcttatc
    15301 ggcccgaccg gatcgggtaa aaccctgctg gcgcaatctt tggcgcgcaa actggatgtg
    15361 ccgttcgtga tggcagatgc aaccacgctg accgaagcag gttatgtcgg cgaagatgtc
    15421 gaacagatta ttaccaaact gttgggcaaa tgtgatttcg atgtcgaaaa agcccaacgc
    15481 ggcatcgtct atatcgacga aatcgataaa atttcacgca aaagcgacaa tccgtccatc
    15541 acgcgtgacg tgtccggcga aggcgtgcaa caagccttgc tgaaactgat tgaaggtacg
    15601 gtggccagcg ttccgcccca aggcggccgc aagcatccga atcaggaatt tatcaacgtc
    15661 gataccacca acatcctgtt tatctgcggc ggcgcgtttg caggcttgga aaaagtcatc
    15721 cgccagcgta ctgaaaaagg cggtatcggt ttcggtgcgt ccgttcacag caaggacgaa
    15781 aatgccgaca ttaccaagct gttcggcatc gtcgaaccgg aagatttaat caaattcggt
    15841 ctgattccgg aattaatcgg acgtttgcct gtgattgcaa cgttagaaga actggatgag
    15901 gacgcgctga ttaatatttt gaccgagccg aaaaacgctt tggtcaagca gtatcaagcc
    15961 ttgttcggta tggaaaatgt cgagttggaa tttgaagaag gcgcattgcg ttccattgcg
    16021 tggcaggcaa tggagcgcaa aaccggcgcg cgcggcctgc gttccatcgt cgaacgctgc
    16081 ctgctcgata cgatgtaccg cctgcctgat ttgcaaggct tgaaaaaagt ggtggtcggt
    16141 aaggcagtca tcgaagaggg caaggaaccg gaattggtgt tcgagtcctg atggcaaaca
    16201 aaaccgcttc ggaacgttga accacgtttc cgaagcggtt ttttgttttt gatgcctagg
    16261 ggcggtttgc ggcggcaaat tccgtcagca cgtgttccaa acgccgcaaa ccttccgccg
    16321 tatcctcatc gtccaacagc agcgaaggcg cgaaacgcaa tacgtccgca cccgcaacca
    16381 aaatcatcac gccgtgtttc aaggcggcgg cggtgatttc ggatgcgcgt ccgcgatagg
    16441 cttcgtccaa cacgcagccg agcagcagtc ccatgccgcg aacctgtgag aacaagcctg
    16501 ttttcctgcc caaatccagc aatgccgtct gaagtttctg cccctgttca cggacatggt
    16561 ttaaagtttc gggcgtattg atgatgtcga atgcgcggct gccgaccgca cacgccatcg
    16621 ggttgccgcc gaaagtcgag ccgtgcgtgc ccggttggaa ggcggcggca atcttttctg
    16681 tcgccagcat cgcgccgatc ggaaagccgc agcccaaggc ttttgccgaa ctcaaaatat
    16741 cgggaacaat gccgtaatgt tcataggcaa acagcctgcc cgtatgcccc atcccggttt
    16801 gaacttcgtc caaaatcaac aacgcattgt gccggtcgca cagacggcgc gcggtttgca
    16861 aatattccgc agtggcgggc aggatgccgc tttcgccctg tatcggctcg atgatgaccg
    16921 cgcaggtctg ttcgccgacg gcagcttcca gcgcggcaat atcgttgaac ggaacgtgcg
    16981 taatgccttg cggcagcggt gcataatcct tgctgtattt cggctggccg ccgacggaca
    17041 cggtaaacag cgtgcgcccg tggaaactgt tgatacaggc gacgatttcg cttttgcctc
    17101 cgccgaaacg gtcgcgcgca tatttccttg ccagcttcaa cgccgcctca ttcgcctccg
    17161 cgcccgagtt gcagaaaaaa accttgtcgg caaaactgtt tgcaaccaat ttttgcgcca
    17221 attcctgcgc cggacgcgtc gtatagatat tggaaatgtg ccacagcttg tgcatctgcg
    17281 cgtttaaagc atcgacaagg gcagggtggc agtgtcccag cgcattgacg gcgataccgc
    17341 ctgaaaaatc aatatattca cgcccttccg tatcccaaac gcggctgcct gaagcgcgtt
    17401 cgggaatcat cggggcaaag gcgaaattgg gggtcagata gttttgcata attgctcctg
    17461 attgcggttt tgctcgggat tatgcaccgt ttttcaaagc agataaaggg aaattgccga
    17521 caatatcggg cagcggtaaa aataaaccga aatattttca atcggataga tgaaaaatca
    17581 aaaaaatttc gataaaatgc ttgaggcgcg gcagcgtttt gatttagaat ctccccaata
    17641 tttttatctt cagacggcat ttcatcatgc aaggcaaaaa atttacaacc gcttattgct
    17701 attggtacca cacagacgtg cggtactttt ttgcgcttgc ttgaaatcca tcccatacgc
    17761 aaaacaggcc gctcgaaaac ggggcggctt ttttgttacc ttcacacagg tcgcccgatt
    17821 cggcaacaca cgacagaatc aggaaaatga ccatgaatgc aatgccccaa accgccgcac
    17881 aaaacgaaac cctgcacatt tgcagcatta ttgtcgatga tgcacaggat ttttccccca
    17941 tgcttctcga tgcagggcgg gacgacacac ttatcagcga atcgatgatt ccgcaaatcc
    18001 gcactgttgc cgccctgata gccgccgaac ggcacgactt cagccgcagc agccccgccg
    18061 agtttaccga tgccgccgat ttttttgccg cacggattct cgtattgggc gtgcgacgct
    18121 tccatctgga cgtatcgctg cttcagattt tgaaaaccgc caacagccgc gcccgccgct
    18181 ttgccgaaaa acaccgcctg tttttcacac ccgcacaagc cgaattgagc ctgaacaaac
    18241 acaaaaaccg ccgcctgctg accgtagaaa ccgaacacga agttgaaaac aaaggcaacc
    18301 cagtcgccaa cagcctcgca tttgtccgca aactgcacac cctgcccctg taaacatcac
    18361 gatttgtcgg cagaatgctt gccaaaattc ccgacggcga tacaataaaa ccgattttcc
    18421 acccaaacag gacacacatg aaaaaaatca tcgcctccgc gcttatcgca acattcgcac
    18481 tcgccgcctg ccaagacgac acgcaggcgc ggctcgaaca gcagcagaaa cagattgaag
    18541 ccctgcaaca gcagctcgca cagcaggcag acgatacggt ttaccaactg actcccgaag
    18601 cagtcaaaga caccattcct gccgaagcac aggcaaacgg caacaacggg caacccgtta
    18661 ccggtaaaga cgggcagcag tatatttacg accaatcgac aggaagctgg ctgctgcaaa
    18721 gcctggtcgg cgcggcggca ggcgcgttta tcggcaacgc gctggcaaac aaattcacac
    18781 gggcaggcaa ccaagacagt cccgtcgccc ggcgcgcgcg tgcagcctac catcagtccg
    18841 cacgccccaa tgcgcgcacc agcagggatt tgaacacgcg cagcctccgt gcaaaacaac
    18901 aggcggcgca ggcgcagcgt taccgcccga caacgcgccc gcccgccaat taccgccgcc
    18961 ccgctatgcg cggtttcggc aggaggcggt aaacccggcg caccaatgcc gtctgaagag
    19021 ctttcagacg gcatttttgc atttgttagg gacattgtta tgttgccgtt tgattttcag
    19081 acggcatttt gtttccaagc gtttgatgtc gggatggcaa ttctgattta cgtgcgttgg
    19141 cagggcggca ttgtcatgcc gccgttgggt tttcagacgg catcatatcg aacagtcaag
    19201 ccgtagggcg agcattcttg cccaccgttg caagggcggg atttggtggg caggaatgcc
    19261 caccctgcat ctgaaaccct tccgtgtcat tcccgtgcag gaaggaatct agatatttga
    19321 atttacggaa attttcaatc gttgccaaag cggaaaaggg ttt
//
LOCUS       DAXIEB010000031        18512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000031 DAXIEB010000000
VERSION     DAXIEB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.031"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(108..1211)
                     /gene="dnaN"
                     /locus_tag="ACFOA3_000876"
     CDS             complement(108..1211)
                     /gene="dnaN"
                     /locus_tag="ACFOA3_000876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6897905.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1446..3002)
                     /gene="dnaA"
                     /locus_tag="ACFOA3_000877"
     CDS             complement(1446..3002)
                     /gene="dnaA"
                     /locus_tag="ACFOA3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6897906.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAVSKTAADILAERMKNLPHEPRQAAGSASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3295..3429
                     /gene="rpmH"
                     /locus_tag="ACFOA3_000878"
     CDS             3295..3429
                     /gene="rpmH"
                     /locus_tag="ACFOA3_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6897907.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3432..3797
                     /gene="rnpA"
                     /locus_tag="ACFOA3_000879"
     CDS             3432..3797
                     /gene="rnpA"
                     /locus_tag="ACFOA3_000879"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6897908.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3863..4090
                     /gene="yidD"
                     /locus_tag="ACFOA3_000880"
     CDS             3863..4090
                     /gene="yidD"
                     /locus_tag="ACFOA3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6897909.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHGHDPVP"
     gene            4263..5900
                     /gene="yidC"
                     /locus_tag="ACFOA3_000881"
     CDS             4263..5900
                     /gene="yidC"
                     /locus_tag="ACFOA3_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6897910.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDNLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(5981..6694)
                     /locus_tag="ACFOA3_000882"
     CDS             complement(5981..6694)
                     /locus_tag="ACFOA3_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6897911.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAVVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6753..7343)
                     /locus_tag="ACFOA3_000883"
     CDS             complement(6753..7343)
                     /locus_tag="ACFOA3_000883"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6897912.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7491..7994
                     /locus_tag="ACFOA3_000884"
     CDS             7491..7994
                     /locus_tag="ACFOA3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6897913.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            8028..8207
                     /gene="rpmF"
                     /locus_tag="ACFOA3_000885"
     CDS             8028..8207
                     /gene="rpmF"
                     /locus_tag="ACFOA3_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6897914.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8382..8909
                     /locus_tag="ACFOA3_000886"
     CDS             8382..8909
                     /locus_tag="ACFOA3_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6897915.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8997..10052
                     /gene="plsX"
                     /locus_tag="ACFOA3_000887"
     CDS             8997..10052
                     /gene="plsX"
                     /locus_tag="ACFOA3_000887"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6897916.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10188..10439
                     /locus_tag="ACFOA3_000888"
     CDS             10188..10439
                     /locus_tag="ACFOA3_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6897917.1"
                     /translation="MKTLEKRMKALDKRIMKFEKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10466..10882
                     /locus_tag="ACFOA3_000889"
     CDS             10466..10882
                     /locus_tag="ACFOA3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897918.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KAGVLVFRIIPEPEFGHTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            10902..11105
                     /locus_tag="ACFOA3_000890"
     CDS             10902..11105
                     /locus_tag="ACFOA3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897919.1"
                     /translation="MPSEACLVFRRHFLRLKQHIGNPAPPVHGNLHLKTICMPNHTPI
                     PISVICTLPVLGNHRFFAYNNRI"
     gene            11132..12094
                     /locus_tag="ACFOA3_000891"
     CDS             11132..12094
                     /locus_tag="ACFOA3_000891"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6897920.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQRVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12141..12548
                     /locus_tag="ACFOA3_000892"
     CDS             12141..12548
                     /locus_tag="ACFOA3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6897921.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAIRGQRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            12642..13568
                     /gene="fabD"
                     /locus_tag="ACFOA3_000893"
     CDS             12642..13568
                     /gene="fabD"
                     /locus_tag="ACFOA3_000893"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6897922.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQSEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            13707..15572
                     /gene="msbA"
                     /locus_tag="ACFOA3_000894"
     CDS             13707..15572
                     /gene="msbA"
                     /locus_tag="ACFOA3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6897923.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCVFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15672..17237
                     /gene="guaA"
                     /locus_tag="ACFOA3_000895"
     CDS             15672..17237
                     /gene="guaA"
                     /locus_tag="ACFOA3_000895"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6897924.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFNLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTIPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17299..18045
                     /gene="fabG"
                     /locus_tag="ACFOA3_000896"
     CDS             17299..18045
                     /gene="fabG"
                     /locus_tag="ACFOA3_000896"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6897925.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(18307..>18512)
                     /locus_tag="ACFOA3_000897"
     CDS             complement(18307..>18512)
                     /locus_tag="ACFOA3_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6897926.1"
                     /translation="ETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQ
                     SHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         4635 a         5622 c         4406 g         3849 t
ORIGIN      
        1 ggtttttgtt aatccactat acctttccga cagccgaaca aaaccccgaa tccgtctgca
       61 cggttcgggg tatatctcca atacgggcat cgtgttccgg aaaaccgtca aatccgcatc
      121 ggcatcacaa tatatttgaa attcggattg ttcggcacgg taaacagcgt cgagcggttg
      181 gcatcgccga aggcaagctg catatcgtcg gaatggatgt tgcgcaacac gtccatcaga
      241 tagccgatat tgaaaccgac ttcgagttcg ccaccctgat aggcgatttc gatttcttcg
      301 cgcgcttctt cctgctcgtt gttgctgcac acgacgctca acaggccgga ttgcaggaac
      361 aggcgcgcgc cacggaattt ttcgttggca agaatggaca cacgttccag cgcacccaaa
      421 agttcggcac gggacaaaac gaaaatcttg tcgttgtcca aaggaatcac gcggttgaaa
      481 tcggggaatt tgccgtcgat gaccttgctg acgatggtcg tgccgttgca ttggaaacgc
      541 acctgtttgt ccagcagctc gatttgaatc ggatcgtcgg ggttgttcaa cagtttgaac
      601 agttccagca ccgttttgcg cggcaaaatc acttcggcgt gcggcaaatc cgcatcaatc
      661 gcgcaggctg cataggcaag gcggtgtccg tcggtcgcca caaggcgcaa ctggctgccc
      721 tcaacctgca tcagcagacc gttgagataa tagcggatgt cctgcaccgc catgctgtac
      781 tgcacttgcg acagcatggt tttgaaacgc tcctgctcca gcgagaaagt cgcgctgatg
      841 tcctcgccga cattcatcat cggaaaatcg gcggcaggca gggtctgcag ggcaaaacgc
      901 gatttgcccg ccttcagcgt cagacggctg tcgtcccaat ccagcgacac cagcgcaccg
      961 gcaggcagcg cgcgcaaaat gtcctgaaat ttcttggcat tggtagtgat gcggaagtcg
     1021 cccgcgccgc cctcgggacc cgcagtgtcg atttggattt ccaaatcggt tgccaagagt
     1081 ttggtctgac cgccttttcc ctcaatcagg acgttggaca ggatgggcag ggtgtggcgg
     1141 cgttcgacga tgccggtaac ggcttgcagc ggcttgagca ggctgtcgcg ctcggcttgt
     1201 aaaatcaaca tgttcgctcc tttaaatcgg tttgtatagt ggattaaatt taaatcagga
     1261 caaggcgacg aagccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag
     1321 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttaaagtt
     1381 aatccgctat atctttaccc ttcggacggc atgggcaata tcatgtcgtc tgaaaacgtt
     1441 ttccatcagt tttgaatcag aatcagcagc ttttcataat cctgagccaa ttccggatct
     1501 tcttcgcgca gtttcgccac tgccctgatg ccgtgcataa cggtcgtatg gtcgcgtccg
     1561 ccgaacgaat cgccgataga cggcaggctc aaagtggtca gttctttggt caggctcatc
     1621 gccacctggc gcggacgggc aatgtttcgt gtccgtttct taccgagcac atcgctgatt
     1681 ttgatgcggt aatatttcgc caccgcatcg atgatgatat cggcggtgat gactttgtgc
     1741 ttctcggcaa taatgtcctg caaagcggta cgcgccaaat cgatgtcgat gacgggacgg
     1801 ttcataaagc ggctgctcgc tccgacgcgg ttgaacgcgc cttcaagctc acgcacgttg
     1861 gaacggatca gattggcaat gaacagcgcg gcttcgtctt caatactgat gcccgccgct
     1921 tccgcctttt tctgcaaaat ggcgatacgc atttccaatt cgggcggctc gagttccaaa
     1981 gtcagtcccc atgaaaaacg ggatttgagg cggtcgtcca taccttcgat tttggcgggt
     2041 aaaacatcgc aagtgaggat gagctgtttt ttctcgttgt ggaaatggtt gtacagatag
     2101 aagaattcct ccatcgtacg gtcttttcct ttaataaact gaatatcgtc gataatcagc
     2161 aggtcgtatt gcttgtattg ctgcttgaac acgtcgtagg tattgttgcg aaccgccttc
     2221 ataaaactgc ggatgtagtc gtccgaatgc atatagcgca ctttggcatc gggacggttt
     2281 ttcaacagtt cgttgccgac cgcctgcaca aggtgggttt tacccaaacc cgtactgcca
     2341 tagaggaaga acgggttgta actctgcccc gggctttccg caatcgcctg cgccgccgcc
     2401 gccgcaaggc ggttgccctt gccttcgacc aatgtgtcga acgtataatc cggcgacaga
     2461 ttggtctgct cgtaacgcgc ctcttccgca tcgcgctgtg catccgtccg tgctttggca
     2521 actgccgccg attccggccg ggaagcagac ccggcagcct gacgcggctc gtgcggcagg
     2581 tttttcatac gttctgccaa aatatccgcc gccgttttcg acacagcggg tttgacaggc
     2641 tcttcagacg gcagctcgtc caacagaacc tcctgcacag gcatctcctc cgaccccgcg
     2701 tgcaaggacg gctcggcagg ttcgacagca ccttcaaccg ccgccatctc ataacgtacg
     2761 ccttctcccg gtttgaatac gaaggcggaa cggccggcag ccaactcttc cctcaccgct
     2821 tctatttttc cggcaaactg gctcttgagc atattgcagg caaactgatt cttgccgtac
     2881 accacccata cgccaccctc ctcaccgacg gtaaggggcg caatccattg cgcaaactgc
     2941 ccttgaggca acatatcgtg aagacggcgg aggcacagcg gccaaaactc tgctaatgtc
     3001 atggataagc tcgaatcggt aaaaatgaaa tcgaaaacaa agaaaatata atattttcaa
     3061 aaagaaaaca aatctgttga acacacatcg gttcaaaatg cagcgaaccg aattataccg
     3121 actcgccgat attttatcca caacccgtgc aaaatttatc cacagaaagg cggcagaaat
     3181 ccgcaggcaa tcgggcaatc ctcctgcaaa gtttctatat tgattgacaa aagcggcaaa
     3241 ttggagtgta attcacggtt taattatcta cccattctat ttttaggaaa catcatgaaa
     3301 cgcacttatc aaccttccgt taccaaacgc aaacgcaccc acggcttcct ggtgcgctcc
     3361 aaaacacgcg gcggccgcgc agtattggcc gcacgccgtg ccaaaggccg caaacgcctg
     3421 gcggtataat tttggactac cgcttcggaa ggcagtaccg cttgttaaaa acggatgatt
     3481 tttcatccgt ttttgcattc agaaaccgcc gcagccgcga cctgctgcaa gtttcgcgtt
     3541 caaacggcaa cgggctggac catccccgca tcggtctggt ggtcggcaaa aaaaccgcca
     3601 aacgcgccaa cgaacgaaat tatatgaagc gcgtcatccg cgactggttc agattgaaca
     3661 aaaaccggct gccgccgcag gatttcgtcg tgcgcgtccg ccgtaaattc gacagggcta
     3721 ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt cggcaacccc gcaaccggat
     3781 gcaggaaaca ggcatgatca gaacggtact ctgcaggcaa ggttcagacg gcaacgggtt
     3841 tcccatacta aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
     3901 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     3961 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggtcggct
     4021 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg gacacgaccc
     4081 cgttccctga cccgacgcaa tattcaaatt gcacgctttc cttttatttc ccatcggttt
     4141 ctatataatg ccgtctgaac cttcgggctg gcggcacgac cgtcgggtat gaagcccgcc
     4201 cttattcccc gtctatcgga acacgcaacc tgcggcattt ccgaccattc aggaaactct
     4261 atatggattt taaaagactc acggcgtttt tcgccatcgc gctggtgatt atgatcggct
     4321 gggaaaaaat gttccccacc ccgaaacccg tccccgcacc ccaacagacg gcacaaaaac
     4381 aggcagcaac cgcttccgcc gaagccgcgc tcgcgcccgc aacgccgatt accgtaacga
     4441 ccgacacggt tcaagccgtc attgatgaaa aaagcggcga cctgcgccgg ctgaccctgc
     4501 tcaaatacaa agcaaccggc gacgaaacca aaccgttcgt cctgtttggc gacggcaaag
     4561 aatacaccta cgtcgcccaa tccgaacttt tggacgcgca aggcaacaac gttctgaaag
     4621 gcatcggctt taccgcaccg caaaaacaat acagcctcaa cggcgacaaa gtcgaagtcc
     4681 gcctgagcgc gcccgaaacc aacggactga aaatcgacaa agtctatacc ttcaccaaag
     4741 gcagctatct ggtcaacgtc cgcttcgaca tcgccaacgg cagcggtcaa accgccaacc
     4801 tgagcgcgga ctaccgcatc gtccgcgacc acagcgaacc cgagggtcaa ggctacttta
     4861 cccactctta cgtcggccct gttgtttata cccctgaagg caacttccaa aaagtcagct
     4921 tctccgactt ggacgacgat gccaaatccg gcaaatccga ggccgaatac atccgcaaaa
     4981 ccccgaccgg ctggctcggc atgattgaac accactttat gtccgcttgg attttgcagc
     5041 ctaaaggcgg acaaagcgtt tgcgccgctg gcgactgccg tattgacatc aaacgccgca
     5101 acgacaacct gtacagcacc agcgtcagcg tgcctttagc cgctatccaa aacggcgcga
     5161 aatccgaagc ctccatcaac ctctacgccg gcccgcagac cacatccgtc atcgcaaaca
     5221 tcgccgacaa cctgcaactg gcaaaagact acggcaaagt acactggttc gcttccccac
     5281 tcttctggct cctgaaccaa ctgcacaaca tcatcggcaa ctggggctgg gcaatcgtcg
     5341 ttttgaccat catcgtcaaa gccgtactgt atccattgac caacgcctct taccgctcta
     5401 tggcaaaaat gcgtgccgcc gcacccaaac tgcaagccat caaagagaaa tacggcgacg
     5461 accgtatggc gcaacaacag gcgatgatgc agctttacac agacgagaaa atcaacccgc
     5521 tgggcggctg cctgcctatg ctgttgcaaa tccccgtctt catcggattg tattgggcat
     5581 tgttcgcctc cgtagaattg cgccaggcac cttggctggg ttggattacc gacctcagcc
     5641 gcgccgaccc ctactacatc ctgcccatca ttatggcggc aacgatgttc gcccaaactt
     5701 atctgaaccc gccgccgacc gacccgatgc aggcgaaaat gatgaaaatc atgccgttgg
     5761 ttttctccgt catgttcttc ttcttccctg ccggtctggt attgtactgg gtagtcaaca
     5821 acctcctgac catcgcccag caatggcaca tcaaccgcag catcgaaaaa caacgcgccc
     5881 aaggcgaagt cgtttcctaa atgccgcagc atgaaaaatg ccgtctgaaa cctgttcaga
     5941 cggcattttt attgctcacc ccctatcggg gcggaaatct tcaacctgca tacatcacaa
     6001 aaatcgtcgg gcgttttttc agattgggca tttcttttct ttttcgccac tgcacaaccg
     6061 tctggctgat gatttcctga gtcggcaacg tcaaatccgt agccgtgcac aagcgcgttt
     6121 caggatgcag gttttccacc gcatccgcca acaacacatc gttgcgatac ggcgtttcga
     6181 taaacagctg cgtttcattc tgctggcgcg aacgttgctc caaagccttc aaactctgaa
     6241 tccgctcgtt tttttcagac ggcagataac ccttaaacgc aaaactctgc ccgttcgcac
     6301 ccgaagccat caaagccagc agcaggctgg aaggcccaac cagcggacgc acttcaaaac
     6361 cgtgtttatg cgccaaagcc accagattcg cacccggatc ggcaacagcc gggcaacccg
     6421 cctcgctgac aatgcccata ctgcgccctt cttgcaaagg tttcagcaat tccggcaaag
     6481 tcttcaaatc cgtgtgttca ttcagcgttt gcaaattcag ttcgcggata ggcattgtca
     6541 cgcccaaatg tttcaaatgc gcgcgcgccg ttttttccgc ttccacgaca aaatccgtca
     6601 gtccgacaac cgcctgctgt tcgtgcggca acaggcacgg cgtgtcgggc gtgcccaaag
     6661 gcgtaggaat caaatacaaa acaggagaca tcattccctc actcgtcagt taaaaatgcc
     6721 gtccgaaccc ttcagacggc ataaacgggc agttacagaa cctccacgcc ctcatttttc
     6781 aagaaatcga ccagacggaa aaccggcaaa ccgattaaag cattcggatc gctgctttca
     6841 atcctttcga tcaacaatgc acccaaagcc tcgctcttca acgcacacga gcaataaacc
     6901 gcatcaggct cgcgctccaa atagcggagg atgtgtagct cgtccaactt cctcatcacg
     6961 accaccgtct tatcaatatg ccgctgcatc ctgcccgtaa ccgtatttag cagaacgacc
     7021 gcgctgtaaa actcaatctc cctgccgctc aaatgcatca acatcttttg cgcattagca
     7081 aggttcatcg gcttgcccca ctgcctgccg tcgcaccacg ccacctggtc cgcaccgaca
     7141 atcaacgcct cggggaaacg tcctgccaac gaccgcgcct taccctcggc aaggcgcaat
     7201 gccgtctgag gggcagactc ccccaacatc ggcgtttcgt caaaatcggg ggacgccgcc
     7261 tgaaaggcaa tgccgagcct ttccatctgt tcgcggcgga aaaccgaact cgtacccaaa
     7321 atcaaaggca gttccaaacc catcccatcc tccttaccgt tgaaaacacg cccgaagggg
     7381 cggtaaaatc cagccatgcg ccgaaacacg gatacccgcc ttcggcgtac cgcaacattt
     7441 ttcttaaaaa tattgacgtt agaacatcta aattatatca tatctcgttt atgtcagacc
     7501 ctaatttgat tgacccggaa attttcgccg ccgaagggca gaatctgcaa ggcagttttc
     7561 tgctggaaga attggatgaa cgcgtcagtt cgcacgatta tcccgccgac aggcagacca
     7621 aaatatcgtt tacactgacc ggcggtcgcg atcggctgca acgcctgttc ctcgacctga
     7681 acgtcaaagc cgatatgccc ctgatttgcc agagatgtat caaacccatg ccgttcatgc
     7741 ttgatgaaag cagccgtatc gtcctgtttt ccaacgaaga gtccttggac gaatccatgc
     7801 ttgccgacga agaactcgaa ggcatactga ttgaaaaaga actcgacgtg cgcacattgg
     7861 tagaagacca aatcctgatg tccctgccct tttcgccgcg acacgaagac tgcggcgaca
     7921 atgggacact ggaagaagtc aatcgggaca aacccaaccc ctttgctgtt ttggcaggtt
     7981 tgaaaagcag ttaattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac
     8041 aaaacaaaaa atccccttcc aaacgcggta tgcaccgttc gcacgacgcg ctgaccgcgc
     8101 ctgcactgtc tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca
     8161 acggtatgta ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact
     8221 gactgaaaaa gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca
     8281 tccgtccaaa ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataatacccc
     8341 gtgaaaatat tccaaaagcc ccaaccacca aggaaattcc gatgaaacag aaaatctggt
     8401 acacctacga tgacatccac cgcgtcatca aagcattggc agaaaaaatc cggaacgccg
     8461 acatcaaata cgatgccatg attgccatcg gcggcggcgg ctttattccg gcacgtatgc
     8521 tgcgctgttt tctggaaatt ccgatttatg ccgtaaccac cgcctattac gacagcgaca
     8581 acgaaggaca ggttaccgaa gaagtcaaaa aagtccaatg gctagacccc gttccagaag
     8641 ccctgcgggg caaaaacgta ctcgtcgtcg atgaagtgga cgacagccgc gtaaccatgg
     8701 aattttgcct gacagagctg ctcaaagaag acttcggtac gatcggcgtc gccgtactgc
     8761 acgaaaaaat caaagccaaa gcaggcaaaa tccccgaagg cattccctat ttcagcggca
     8821 tcaccgtaga agactggtgg atcaactatc cgtgggacgc actcgacatc gacgaacaca
     8881 accgccttgc cgaggccggc cgaggctgac cctttcagac ggcatatttt ccgaaccgat
     8941 gccgtctgaa gcccgcacga cccctgccgc agaccgaaaa cctaccggag aaccctatga
     9001 ttacacttgc cgtagatgcc atgggcggcg accaaggact tgccgttacc gtacccggcg
     9061 caaccgcatt cctccaagca caccccgatg tccgcctgat tatgaccggc gacgaaacgc
     9121 aactgcgcca agccctgacc gcggcaggcg caccgatgga acgcatcgac atctgccata
     9181 ccacccaagt tgtcggcatg gacgaagccc cgcaatccgc cctgaaaaac aaaaaagact
     9241 cctccatgcg cgttgccatc aaccaagtta aagaaggcaa agcccaagcc gccgtatccg
     9301 caggcaacac gggcgcgctc atggcaaccg cacgtttcgt cctcaaaacc attcccggca
     9361 tcgaacgccc cgccatcgcc aaattccttc cttccgacac cgaccacgtt accctcgccc
     9421 tcgacctcgg cgcaaacgtc gactgcacgc ccgaacagct cgcccaattt gccgttatcg
     9481 gcagcgaact cgtccacgca ctccatcctc aaaaaggaca gccgcgtgtc gggctggtca
     9541 acgtcggcac ggaagacatc aaaggtacgg acaccgtcaa acaaacctac aaactgctgc
     9601 aaaacagcaa actcaacttt atcggcaaca tcgaaagcaa cggcatcctg tacggcgaag
     9661 cagatgtcgt cgtcgccgac ggctttgtcg gcaacgtcat gctcaaaacc atcgaaggcg
     9721 cggtcaaatt catgagcgga gccatccgcc gcgaattcca aagcaacctg ttcaacaaac
     9781 ttgccgccgt tgccgcccta cccgccctca aagggctgaa aaacaaactc gatccgcgca
     9841 aattcaacgg ggccatcctg ctcgggctgc gcggcatcgt catcaaaagc cacggcggca
     9901 cagacgaaac cggtttccgc tacgcactcg aagaagccta ccacgaagcc aagtccgccg
     9961 gcctttccaa aatcgaacaa ggcgttgccg aacaactcgc cgcactcgaa actgccaaag
    10021 ccgtccaaaa cgaaaatgcc gacggtctgt aacacacacg atgccgtctg aacgcccccg
    10081 cccctttcag acggcatccg cccgcaccaa acctgcgggc gcggacggcg atgcgcctgt
    10141 ccggcacttc ccaaatactt ccctgtaaaa taaggagtat ttgaaaaatg aagacattag
    10201 aaaaacggat gaaagctcta gacaaacgga ttatgaagtt cgaaaaatcc cttgaaggca
    10261 ggcttgatgc ccgtctgatt gaatccgcat tggattatat tcattattcg gaacgttttt
    10321 tggcttttga aatcctgtgt acttatatcg aagatttcga tgtccggctg acggaacaag
    10381 aatcccggga aatttctttt atcaacaagg aatttgagat agaaagcacg tccgattaac
    10441 caataaagcc aatgggttga taaacatgaa aacatcgacg gtcgtttttg gcggattttt
    10501 tatggcagac aacggagagc gaatccaaat ccccgttttg gaaaatcctg acattaggga
    10561 aatcaatcac tttttttccg tatcaaattt tgagaaaaaa gccggcgtcc ttgttttcag
    10621 aatcatcccc gagccggaat ttggccatac cgaattaact gtctattttg aaaaaggata
    10681 ttatttgcca atgattcaaa caattttaga aggcggggat attgaagtca aaaaccttaa
    10741 aacggaaaat tacagtggga aggcaaggga aattttggga gattcctacc ctattgagca
    10801 tatctctaaa aatatttcta caattcaaaa tataatttct gaatttatga tacaaaaaca
    10861 aacaccggca ccaataattt gatggaaaac caacaatcaa aatgccgtct gaagcatgtt
    10921 tggttttcag acggcatttc cttcgcttaa aacagcatat cggcaacccc gccccgcccg
    10981 tccacggcaa tctgcatctg aaaaccatct gtatgccaaa ccacaccccc atcccgattt
    11041 ccgtcatttg caccctgccc gtattgggca atcatcggtt tttcgcttac aataaccgaa
    11101 tctgaaccaa ctatccaaaa aggccgttcc catgcagtat gccaaaattt ccggcacggg
    11161 cagctatctt cccgccaacc gcgtcagcaa tgacgacctt gcccaaagag tggatacctc
    11221 tgacgagtgg attaccgcgc gcacgggcat caagttccgc catattgcag ccgaaaacga
    11281 aaaaaccagc gaccttgccg ccgaagcggc gcgccgcgcg ctggatgcag ccggattaga
    11341 cagtggcgaa atcgatttga ttatcgtggc aacggcaacg ccggatatgc agtttccttc
    11401 taccgccacc atcgtgcagc aaaaattggg catcaccaac ggctgccccg cgtttgacgt
    11461 acaggcggtg tgcgcaggct ttatgtacgc gctgaccacg gcaaacgcct acattaaaag
    11521 cggtatggcg aaaaacgcgc tggtcatcgg cgcggaaacc ttcagccgca tcgtcgattg
    11581 gaacgaccgc acaacctgcg tattgttcgg cgacggcgcg ggcgcggtgg ttttgagcgc
    11641 ggcggacaaa ccgggcatca tccacagcaa actcaaagcc gacggcaatt atctgaaact
    11701 cttaaacgtc cccgggcaaa tcgcctgcgg caaagtttcc ggttcgccgt acatttcgat
    11761 ggacggtccc ggcgtgttca agtttgccgt caaaatgctg tccaaaatcg ccgatgacgt
    11821 tatcgaagaa gccggttata ccgccgctca aatcgactgg atcgtgccgc atcaggcaaa
    11881 ccgccgcatt atcgaatcga ccgcgaaaca tttaggtttg agtatggaca aagtcgtcct
    11941 gaccgtccaa gaccacggca acacatccgc cgcatcgatt ccgctggctt tggatacggg
    12001 catccgcagc ggacaaatca aacgcggtca aaacctgctg ctcgaaggca taggcggcgg
    12061 tttcgcgtgg ggcgcggtgc tgttgcaata ttgaacccga tgccgtctga aacaggcttt
    12121 cagacggcat ttcccatatc atgaagcggc aggctttctt caaactgatg gcgtgtgcgg
    12181 catttctgtc tgccgtttcg ctgcgcctcc ccgtattggg cgcgtgttac gcaatattgt
    12241 ccctctatgc gtttgcactt tacggcatcg acaaacggcg cgccatacgg gggcaacgcc
    12301 gcattcccga acaccgcctg ctgctgcctg ccttgttcgg cggttgggcg ggcgcatact
    12361 tgggcagcag gatattcagg cataaaacgg cgaaaaagcg ttttgttgtg ctgttccgtc
    12421 tgactgtttc gggcaatgtc ctggcgaccc tcatcctgat ttatagtgga ttaaatttaa
    12481 accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct
    12541 tgtcctgatt tttgttaatc cactatatta ttttgtcccg cctgaatttt tcgtaaaact
    12601 cgggcagaat acctgattat ccaaccaaac aaaggaatac tatgtctttt gccttctttt
    12661 ttcccggaca aggttcccaa agcctcggta tgatgaacgg ctttgccgaa cacgccatcg
    12721 tcaaaaacac ctttgccgaa gcctccgcca tattggggca ggacttgtgg gcgatgataa
    12781 acggcagcga tgccgaaatc atcggtcaaa ccgtcaacac ccagcccatt atgctcgccg
    12841 ccggcgttgc cgtttaccgc gcctatttag aagcgggcgg caaaacgcct gccgccgttg
    12901 ccggacacag cctcggcgaa tacaccgcac tcgttgccgc cggcgcattg aattttgccg
    12961 acgcggtcaa actcgtgcgc ctgcgcgccg aactgatgca gtccgccgta ccgcaaggcg
    13021 tgggcgcaat ggcggcgatt ctcggcttgg aagatgagca ggttaaagcc atttgtgccg
    13081 aagccgccca aagcgaagtg gtcgaagccg tcaacttcaa ctcacccgga caaatcgtga
    13141 ttgcaggcaa cgccgccgcc gtcggacgcg ccatggctgc cgccaaagaa gccggtgcca
    13201 aacgcgccct gccgctgccc gtgtccgtac cttcccattg cagcctgatg aaacccgccg
    13261 ccgacaaact tgccgaagcc ctgaaaaccg ttgaaatcaa gcagccgcaa atccgcgtta
    13321 tccacaacgc cgacgttgcc gcctacgatg atgccgacaa aatcaaagac gcgctcgtcc
    13381 gccagcttta cagccccgta cgctggacgg aaaccgtcaa cgccctcgtt tcagacggca
    13441 ttgccgaatc cgccgaatgc ggcccgggca aagtgttggc gggcttggca aaacgcatca
    13501 acaaagccgc cgcgtgcagc gcactgaccg atgccggaca ggttgccgcc tttatcgaaa
    13561 cgcactgact tcgttccgca aaaagcagcc tgcatgcctc aggctgcttt tcacgtccga
    13621 acggcaaaca gccgcatatt tgcgctataa ttgatgacga ccaaaccacc gacagcggct
    13681 gccgttgcag ttcccgccct accgatatga tagaaaaact gactttcgga ctgtttaaaa
    13741 aagaagacgc gcgcagcttt atgcgcctga tggcatacgt ccgcccctac aaaatccgca
    13801 tcgttgccgc cctgattgcc attttcggcg ttgccgccac cgaaagctac cttgccgcct
    13861 tcatcgcccc cctgattaac cacggctttt ccgcacccgc cgcgccgccc gagctgtctg
    13921 ccgccgccgg catcatttcc accctgcaaa actggcgcga acagtttacc tatatggttt
    13981 gggggacgga aaacaaaatc tggaccgtcc cgctcttcct catcatcctc gtcgtcatcc
    14041 gtggcatctg ccgctttacc agcacctatc tgatgacttg ggtctccgtg atgaccatca
    14101 gcaaaatccg caaagatatg tttgccaaaa tgctgaccct ttcctcccgc taccatcagg
    14161 aaacgccgtc cggcaccgta ctgatgaata tgctcaacct gaccgaacag tcggtcagca
    14221 acgccagcga catattcacc gtcctcacgc gcgacacgat gatcgttacc ggcctgacca
    14281 tcgtcctgct ttacctcaac tggcagctca gcctcatcgt cgtcctgatg ttccccctgc
    14341 tctccctgct ctcgcgctac taccgcgacc gtctgaagca cgtcatttcc gactcgcaaa
    14401 aaagcatagg cacgatgaac aacgtgattg ccgaaaccca tcagggacac cgcgtcgtca
    14461 agctgttcaa cgggcaggcg caggcggcaa accggttcga cgcggtcaac cgcaccatcg
    14521 tccgcctcag caaaaaaatc acgcaggcaa cggcggcaca ttccccgttc agcgaactga
    14581 tcgcctcgat cgccctcgcc gtcgtcatct tcatcgccct gtggcaaagc caaaacggct
    14641 acaccaccat cggcgaattt atggcattca tcgtcgccat gctgcaaatg tacgccccca
    14701 tcaaaagcct tgccaacatc agcatcccta tgcagacgat gttcctcgcc gccgacggcg
    14761 tatgtgtatt tctcgacacc ccgcccgaac aggacaaagg cacgctcgcg ccgcagcgtg
    14821 tcgaagggcg catcagcttc cgcaacgtcg atgtcgaata ccgttcagac ggcatcaaag
    14881 ccctcgacaa cttcaacctc gacatcagac aaggcgaacg cgtcgccttg gtcggacgtt
    14941 ccggcagcgg caaatccacc gtcgtcaacc tgctgccccg ctttgtcgaa ccgtctgccg
    15001 gcaacatctg catagacggt atcgacatcg ccgacatcaa actcgactgc ctgcgcgccc
    15061 aatttgccct cgtctcccaa gacgtattcc tgtttgacga cacccttttt gaaaacgtcc
    15121 gatacagccg tcccgacgcg ggcgaagccg aagtcctatc cgccctccaa gccgccaacc
    15181 tgcaaagcct gatcgacgca tccccactcg gactgcacca gcccatcgga tcgaacggca
    15241 gcaacttatc cggcggacag cggcaacgcg tcgccattgc ccgcgccatt ttgaaagacg
    15301 cgccgatatt attattggac gaagccacca gcgcattgga caacgaatcc gaacgcctcg
    15361 tccaacaggc gctcgaacgc ctgatggaaa accgcaccgg catcatcgtc gcccaccgcc
    15421 tgaccaccat cgaaggggcc gaccgcatca tcgtaatgga cgacggcaaa atcatcgaac
    15481 aaggcacaca cgaacaactg atgtcccaaa acggctacta cacgatgtta cgcaatatct
    15541 caaacaaaga tgccgccgtc cggacggcat aaacaaaatg ccgtctgaaa tggtacaatc
    15601 gccccgaccc cttcagacgg catcatatcc gccgacccat ccgattatct tcaatcaccg
    15661 taaaacccat tatgacccaa gacaaaatcc tcatcctcga cttcggttcg caagttaccc
    15721 agctgattgc ccgccgcgtg cgtgaagccc acgtttactg cgagctgcat tccttcgata
    15781 tgcctttgga cgaaatcaaa gccttcaacc ccaaaggcat catcctttcc ggcggcccca
    15841 attccgttta cgaatccgac tatcaggcgg ataccggtat ctttaatttg ggcattccga
    15901 ttttgggcat ctgctacgga atgcagttta tggcgcacca cttgggcggc gaagtgcagc
    15961 ccggcaacca gcgcgaattc ggttatgcgc aagttaaaac catagacagc gagctgacac
    16021 gcggcattca agatggtgag ccaaacacac tcgacgtatg gatgagccac ggcgacaaag
    16081 tgtccaaact gcccgacggt ttcgccgtca tcggcaacac cccgtcctgc ccgattgcca
    16141 tgatggaaaa cgccgaaaaa caattctacg gcatccagtt ccaccccgaa gttacccaca
    16201 ccaaacaagg ccgcgccctg ttgaaccgct ttgtcttgga tatttgcggc gcacaaccga
    16261 gctggacgat accgaactac atcgaagaag ccgttgccaa aatccgcgaa caggtcggca
    16321 gcgacgaagt gattttaggt ttgtccggcg gcgtggactc ttccgtagcc gccgcgctga
    16381 ttcaccgcgc catcggcgac caactgacct gcgtgttcgt cgatcacggg ctcttgcgcc
    16441 tgaacgaagg caaaatggtg atggatatgt tcgcccgcaa cttgggcgtg aaagtgatac
    16501 acgtcgatgc cgaagggcag tttatggcga aactcgccgg cgttaccgat cctgagaaaa
    16561 aacgcaaaat catcggcgcg gaatttatcg aagtatttga tgctgaagag aaaaaactca
    16621 ccaacgccaa atggctggcg caaggcacga tttaccctga cgtaatcgaa tccgcaggcg
    16681 cgaaaaccaa aaaagcccac gccatcaaat cgcaccacaa cgtcggcggc ctgcctgaaa
    16741 acatgaagct caagctgctt gagcctttgc gcgatttgtt caaagacgaa gtgcgcgaac
    16801 tcggcgttgc tttgggtctg ccgcgcgaaa tggtgtaccg ccatcccttc cccggccccg
    16861 gtttgggcgt gcgcatcttg ggcgaagtga aaaaagaata cgccgactta ttgcgtcaag
    16921 cagacgatat tttcatccaa gaattacgca acaccaccga cgaaaacggc acatcttggt
    16981 acgacctgac cagccaggca tttgccgtat tcctgcctgt gaaatccgtc ggcgtaatgg
    17041 gcgacggacg cacttacgac tacgtcgtcg cactgcgcgc agtcatcacc agcgacttca
    17101 tgaccgcaca ctgggcagaa ctgccttact cactgctcgg acgcgtgtcc aaccgcatca
    17161 tcaacgaagt caaaggcatc aaccgcgtgg tgtacgatgt cagcggcaaa ccgcctgcca
    17221 ccatcgagtg ggaataacca gcaaacaggg ctgcaccgtc cggcgcagtc tttcgattat
    17281 cggaaaaaag gaaaaaatat gagcacacaa gatttaagcg gcaaaatcgc tttggtaacc
    17341 ggcgcatcgc gcggtatcgg tgcagcaatt gccgacacac tggcggcagc cggtgccaaa
    17401 gtcatcggta cggcgaccag tgagagcggt gcggcggcga ttagcgagcg gttggcgcaa
    17461 tggggcggcg aaggccgcgt attaaattcc gccgaacccg aaaccgtcga aaacctgatt
    17521 gccgacatcg aaaaaacgtt cggcaaactc gatattctgg tcaacaacgc cggcatcacc
    17581 cgcgacaacc tcctgatgcg catgaaagaa gaagagtggg acgacatcat gcaggtcaac
    17641 ctcaaatccg tgttccgcgc ttctaaagcc gttttgcgcg gtatgatgaa acaacgttcc
    17701 ggccgcatca tcaacatcac atccgtcgtc ggcgtgatgg gcaatgccgg tcaaaccaac
    17761 tatgccgcgg caaaagcagg cttaatcggt ttctccaaat ccatggtgcg cgaagtcggc
    17821 agccggggca ttaccgtcaa ctgcgtcgcc cctggcttta tcgataccga catgacacgc
    17881 gccctgccgg aagaaacccg ccaaaccttt accgcccaaa ccgccttggg cagattcggc
    17941 gacgcgcaag acatcgccga tgcggttctg ttcctcgctt ccgaccaagc aaaatacatc
    18001 accggccaaa cgctgcacgt caacggcggt atgctgatgc cctgacgggc agccgtaccg
    18061 acaatgccgt ctgaagccct ttcagacggc atttgcattc tcaggcaaaa tgaacacacg
    18121 ccacaccccg ccctgcccct gcggatcagg cacaagctga gacctttgca aaactccttt
    18181 ccctcccgac agccgaaacc caaacacagg ttttcggctg ttttcgcccc aaatacctcc
    18241 taattctacc caaatacccc cttaatcctc cccggatacc cgataatcag gcatccgggc
    18301 tgccttttag gcggcagcgg gcgcacttaa cctgttggcc gctttcaaca ggttcaaaca
    18361 catcgccttc aggtggcttt gcgcactcac tttaatcagt ccgaaatagg ctgcccgagc
    18421 gtagcggaat ttacggtgca gcgtaccgaa gctctgttcg accacataac gggtcttcga
    18481 caaatatcgg ttgcgtttgg tttgcgtttc cg
//
LOCUS       DAXIEB010000032        18189 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000032 DAXIEB010000000
VERSION     DAXIEB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.032"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(173..601)
                     /locus_tag="ACFOA3_000898"
     CDS             complement(173..601)
                     /locus_tag="ACFOA3_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6897927.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(666..2414)
                     /locus_tag="ACFOA3_000899"
     CDS             complement(666..2414)
                     /locus_tag="ACFOA3_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6897928.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     IQQTRGCLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYPVGAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLDNWLFG"
     gene            2741..4174
                     /gene="ccoN"
                     /locus_tag="ACFOA3_000900"
     CDS             2741..4174
                     /gene="ccoN"
                     /locus_tag="ACFOA3_000900"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6897929.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4201..4812
                     /gene="ccoO"
                     /locus_tag="ACFOA3_000901"
     CDS             4201..4812
                     /gene="ccoO"
                     /locus_tag="ACFOA3_000901"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6897930.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4817..4987
                     /locus_tag="ACFOA3_000902"
     CDS             4817..4987
                     /locus_tag="ACFOA3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6897931.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            5011..5922
                     /gene="ccoP"
                     /locus_tag="ACFOA3_000903"
                     /pseudo
     CDS             5011..5922
                     /gene="ccoP"
                     /locus_tag="ACFOA3_000903"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5998..6342
                     /locus_tag="ACFOA3_000904"
                     /pseudo
     CDS             5998..6342
                     /locus_tag="ACFOA3_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6544..7953
                     /locus_tag="ACFOA3_000905"
     CDS             6544..7953
                     /locus_tag="ACFOA3_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6897932.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGQEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRAEDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYHTMDEIHSDMMLDSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIQAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPSGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            8054..11263
                     /gene="recC"
                     /locus_tag="ACFOA3_000906"
     CDS             8054..11263
                     /gene="recC"
                     /locus_tag="ACFOA3_000906"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6897933.1"
                     /translation="MFYLYQSNRLETLAALFAHIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPASLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATVDEWVGRCRGLLKTLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQMPEPPQPFFDQSVENAEPVAEIGQDEFIRFWRNPVKAWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQSFQLAAKQIDTAILNSPKLPPLSYAIPSDGQILKGSLSNLYQCGQVFYAY
                     GKPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIIQSGETEVLAEIAQGRALRLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFVQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGAA"
     gene            complement(11344..11975)
                     /gene="mtrR"
                     /locus_tag="ACFOA3_000907"
                     /pseudo
     CDS             complement(11344..11975)
                     /gene="mtrR"
                     /locus_tag="ACFOA3_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
     gene            12223..13461
                     /gene="mtrC"
                     /locus_tag="ACFOA3_000908"
     CDS             12223..13461
                     /gene="mtrC"
                     /locus_tag="ACFOA3_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6897934.1"
                     /translation="MAFYASKAMRAAALAASVALALSSCGKADEAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEASLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRQQVAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAADNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGANWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWMPSENQA
                     AAPQADVQTASEAKPASEAK"
     gene            13473..16676
                     /gene="mtrD"
                     /locus_tag="ACFOA3_000909"
     CDS             13473..16676
                     /gene="mtrD"
                     /locus_tag="ACFOA3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6897935.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPNIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMTTSADSSGSGSVSLTFTPETDEDL
                     AQVEVQNKLSEVLSMLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIDGVGKVQLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVQGQTVTATVTAQGQLSTAEEFGNVILRANTDGSNVYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKAATKKAMGQISGAVIGITAVLMSV
                     FVPLAMFSGATGNIYKQFALTMAASIAFSAFLALTLTPALCATMLKVIPKGHHEEKKG
                     FFGWFNKKFDNWTHGYEGWVAKVLRKTFRMMVIYVGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     MLKDWNERTAPGSDAVSIAGKLTGMMMGTLKDGFGIAIVPPPIMELGNGSGLTINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDAPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVNDFPNQGRLQRVMVQADGDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPAMDLSASPATGVSTGQAMAAVQKMVDEMGGGYSLEW
                     GGQSREEAKGGSQTIALYALAAVAVFLVLAALYESWSIPLAVLLVMPLGLAGAAAGVT
                     GRNLTELMLGGIPAFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLVGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            16731..18131
                     /gene="mtrE"
                     /locus_tag="ACFOA3_000910"
     CDS             16731..18131
                     /gene="mtrE"
                     /locus_tag="ACFOA3_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6897936.1"
                     /translation="MNTTLKTTLTSVAAFALSACTMIPQYERPKVEVAETFKNDTADS
                     GIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLA
                     ANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAA
                     HLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEA
                     LIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSEV
                     LLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWSF
                     APSITLPIFTWGTNKANLDAAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAYA
                     ALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLADL
                     YKALGGGLKRDTQTDK"
BASE COUNT         4212 a         4571 c         4993 g         4413 t
ORIGIN      
        1 tgcattttga aaatgagttt gagcataaaa ttttagtaac ctatgttatt gcaaaggtct
       61 cccactgtaa aaacggagaa ggacagagga cggcagtaag gtttgccgtc ctctttttca
      121 agttgcggtg gaatgccgct tgaaggtgtg gtttaaacgt ttcggcttgt aatcagacgc
      181 gggcgttgtc cagccgctct ctgaagtttt ccaaatttac gcctgccgct tgggcgcggc
      241 ggatgatttc gtcaaccggc ataccttctg ccgcccggcg gaagccccac aggagggagc
      301 agcgcgtacc ggtccggcaa taggcaagga cgggatattc ggcttgtccg atgagttggc
      361 ggaaggtttc gacatcgtgt ttttggatgt cgcgtgcggt aacgggttgg tgatggaatc
      421 cagtaacgcc tgcttgttcc agccactgtt tgatttgggc gaagtcgggt tgcgattctt
      481 cttcgcggtc ggggcggttg cagatgacgg ttttgatgcc cagttgcgcg atttgttccg
      541 catcggcttt ggtcagttgc ggagaaatat agaggtgttc gtcaagtttc agaatggcca
      601 ttttgtttcc tttactttgc ggttgttttt cagacggcat tttgtgtgca atgccgtctg
      661 aaagtttaac cgaacaacca gttgtccaac aggttccggt ctgtgatatg taggatggca
      721 gtatccgctg ccgttgcctg aaccgtcaag acggtttgac gcaatatgcc ggcacggaag
      781 aagtggatat tgatttttgc accgacaggg tatcgggccc attgtgcggc aaagtcggtg
      841 caggcataac cgtctaaagc aatgattttg tcttgcgggc atagtgccgc agattccgcg
      901 ctgccgccgt tgaagacatg ggtcaggacg atgtggtcgg tgttttgttt gaaacgtgcg
      961 ccaaaatcgc ctgccgacgg gacggggcag atgtgttctg cgtatccgcc gccgtgttgt
     1021 cggggaagcg gcaggaaggt cagtcccacg cctgcggttg ccaggcattc ggcaagcggc
     1081 aaatcttcgg tactgtataa cgctttttgg aaaaaatctt ccaaatccaa gccggtaatt
     1141 tcctgacagc ggatttgcca gtgtttttcc ggaatacccg agtgtgtgtc cctccactcc
     1201 cgatagagtt tgtccattat cgtatcgaga gaatgtctgc cgttgcttcg gttgcgtatt
     1261 atcagatcaa ggcacaatgc ggcaagcgcg cctttctggt agtagctgac gatggcgttg
     1321 gggctgtttt catcaggttt gtaaaatttg ttccacgcgg taaaactcga ttccgccaag
     1381 gtctgcctca aacagccgcg ggtttgttgt atgcgcgtaa tgccttgtgc cagcaggttt
     1441 aaataagatt cgggcgagat ggtgcggctg cgtgccaaaa acaaatcgtc gtaataggat
     1501 gtaatgcctt cgaatgccca tagttgttcg gtatagtttt ctttgtcgag gtcataaggg
     1561 gcgaacgcgg caggtttgat ggatttgacg ttccacgcgt gaaaatattc atgggagaaa
     1621 agtccgagca atgtggtgta ggcatcgtcg gcatcggtca taccgaacgg cggaaggctg
     1681 tggcggtcgg cgagcagggc ggtgctgtcg gcgtgttcca aaccgccgta aatatggtcg
     1741 ccgacgtgga gcaggaacaa atatttttga aacggggcag gggaggaaaa caccgccagt
     1801 tctgtttcgc agattttttt gatatccgaa accagcctgt tgcggtcgaa atcgggatag
     1861 atgccgctta aggcaattgt gtgcggaatg cctgccgcct caaaatctaa aaattcaatc
     1921 aagcccatct cgacaggtcg gtcaatcaat tcggcataag atgccgcctg aaagacaaac
     1981 ctccctgttt ccggcagcgt tgtggcaata cgccattcgg acggaatacc cgtcaattcc
     2041 aagcggtgcg gcagcgtttc cgttccttcg actttcaaaa acaggcacga tccgtcaaaa
     2101 aaaccgcgtt ctgtcgtcag gaaagaacct cgaaccgaca aatcgaatgc atatacggtg
     2161 tagcggattt gccactcgcc gcgtacggcg gcagcgtgcc agcggttttt tgcaatttgt
     2221 tcgagcggca tggacgtgcc gttacaggat gcatggatag aagtgatgtg gcgggaaaaa
     2281 tcccgaatca gatagctgcc cggaacccag tttggcaggc ttatttctat aggaagatca
     2341 tcatcttgtg tgaatgtcag caggatgtgc cactcgtggt cgagagggga cggggttatt
     2401 ttataatgaa tcataactat caaatatatc gttaaacggg atggattaaa tacctttaaa
     2461 atgcagctta acctacatca ataaagtgcc acaaaaaggg aaaattggaa acaaaagcaa
     2521 tggcggtcgg gttgtaattt attataacaa tatgatttat atgtaataaa aaacagatta
     2581 agcaaagggc tcgccggcgg cgcgaatgta attccgcttg gcgtaataag gcagatatgt
     2641 taaatttaga aaaaattacg cttgcccgga ttgcacctta aacaggctgc ggaagcggca
     2701 ggataaaaat ctttattccc ataaattcat ggagactctc atggacacac aaacttacaa
     2761 ctacaaagtg gtgcgccagt tcgccatcat gactgtagtt tgggggattg tgggtatgtt
     2821 ggtcggcgtt atcgtcgccg cccagctttt tgctcctgcc ctcgacttgt ctaatatcgg
     2881 accttggttc cacttcggcc gcctgcgtcc gctgcacacc aatgcggtta ttttcgcatt
     2941 tggcggttgc ggcctgatcg gcacatcata ctacgttgtc caacgtactt gtaatacccg
     3001 tcttttcggc ggttggctgc cggcatttac cttttggggc tggcaggcgg taatcgttgc
     3061 cgccgtcgtc agcttcccta tgggttggac ccaaggtaag gaatatgccg aactggaatg
     3121 gccgatcgat attctgatta ctttggtttg ggtggcttac gccatcgtat tcttcggtac
     3181 gattgccaaa cgtaagatta aacatattta cgttgccaac tggttctacg gcggctttat
     3241 tttggcggtc gcacttttac acatcgtcaa caatatcagc atcccggcag ggttgatgaa
     3301 gtcatacccc gtctattccg gtgcgattga tgctatggtt caatggtggt acgggcataa
     3361 cgccgtgggc ttcttcctga ctgccggctt cttgggtatg atgtactatt tcgtacccaa
     3421 acaagcagcc cgccccgttt actcctaccg cctgtccgtc gttcacttct gggcgttgat
     3481 tttcacctat atgtgggcgg gtccgcacca tcttcactac actgcgctgc ctgactggac
     3541 gcaatctttg ggtatggttc tgtctttgat tctgttcgca ccctcttggg gcggtatgat
     3601 taacggcatc atgaccttgt ccggcgcgtg ggacaaactg cgtacagacc cgattcttaa
     3661 attcctgatt gtatccttgt ccttctacgg tatgtctacc tttgaaggcc cgatgatgtc
     3721 gattaaaacg gtcaatgcat tgagccacta tacggactgg accgtcgcgc acgttcatgc
     3781 gggtgcgttg ggctgggtag gctttgtaac catcggttcc gtctattaca tgattccccg
     3841 tctgttcggc aaagaacaga tgcacagcac caagctggta gaagcacatt tttggattgc
     3901 gaccatcggc gtggttctgt atatcgctgc catgtggatt gccggtgtga tgcagggttt
     3961 gatgtggagt tctttgaacg atgacggtac gctgacctac tcgtttgtcg aatccgtaaa
     4021 acgcaccatg ccttactacg tgatccgttt tgcaggtggt ttattgtatc tgagtggtat
     4081 gtgcattatg gcgtacaacg tgtaccgcac agccatcggt ggtaaagcag tcgatgccga
     4141 aatccctgcg gtttcccaaa cacagcacca ctaaaatact aagaaagata ggctaccaaa
     4201 atgaaattac aacaattggc tgaagaaaaa atcggcgttc tgattgtgtt cacgctgctt
     4261 gtagtcagtg tcggtctgtt gattgaagtt gtgcccttgg cctttaccaa ggcggcaaca
     4321 cagccggcgc cgggcgtgaa gccttacaat gccctgcagg ttgccggacg cgatatttac
     4381 atccgtgagg gctgttacaa ctgccactcg caaatgattc gtccgttccg tgcggaaacc
     4441 gagcgttacg gtcattactc tgttgccgga gagtcggttt acgaccatcc gttccaatgg
     4501 ggttccaaac gtaccggtcc tgatttggca cgtgtgggcg gccgctattc cgacgaatgg
     4561 caccgtatcc acctgctgaa tccccgtgat gtcgtgcctg agtccaatat gccggcattc
     4621 ccgtggcttg cacgcaataa agtcgatgtc gatgcaaccg ttgccaacat gaaggctttg
     4681 cgtaaagtag gtactcctta cagtgatgag gaaattgcga aagcgcctga ggctttggca
     4741 aacaaatccg agctggatgc tgtagtcgcc tatctgcaag gattgggtct ggctttgaaa
     4801 aacgtaaggt aacatcatgg atattaacgg tattcgtgct ctcttcacgg tatggatctt
     4861 tatctgtttc ctgttggtac tctatatcgt cttcaacagg cggaataaga aaaactatga
     4921 taatgccgca aacagcattt ttgatgaaaa ccaagatgcg caagataaga aaagcgaaaa
     4981 ccgttaatat tgtgataacg gagcaaaaca atgaacacaa catcccaatt taccagtaat
     5041 ttctggaata tatatattgc agttattgtc ttactgagct ttatcgcttt ggcttggctg
     5101 ctgctgtctc aaaatgttgt caaacgtccg aagaagggcg aagaagtaca aactacgggt
     5161 catgagtggg acggcattgc cgaatacgac aatccgctgc cccgctggtg gttttggctg
     5221 tgtgttttga cgtggctgtt cggtatcggt tatttggtta tgtatccggg tgtcggcgac
     5281 tacaaaggtc tgctgaaatg gaccagccat aaccaatatg aaaaagaggt caaaaaagcc
     5341 gatgagcaat acggcaaact gtatgccaag tttgcggata tgccgattga aaaagtggca
     5401 aaagaccctc aagccaagca aatcgcccaa aacctgttta acacttattg tatccagtgc
     5461 cacggctctg atgctaaagg ctctaaaggt tttccgaatc tgaccgatag cgattggttg
     5521 tggggcggtg atcccgataa aatccacgag accatcgaaa aaggccgtgt tgcgactatg
     5581 cctgcctggg gccctgcttt gggcgaagaa ggtgtgaaag acgttgccca ttatgtgatg
     5641 tccctttcaa aacccgaagg tcagtatgat gaggaacgtg ccgcgcgcgg acaagccttg
     5701 ttcagcggtc cgcctgccaa ctgtttcact tgccacggcg ataagggaca aggtatccaa
     5761 ggtttgggtc cgaacctgac tgatgacgtg tggttgtggg gcggtacgca aaaatccatt
     5821 atcgaaacca ttaccaacgg tcgcagcagc caaatgcccg cttggggaca tttcttggat
     5881 aaagacaaac tgcatattat gactgcttat gtatggggtc tttccgataa agacggtaaa
     5941 gctccggtga aaaaagccga gcctgcaccg actcccgcac cggcggcaga acccgcagcc
     6001 tctgctcctg cagaagcagc acaagccgcg tccgaagcca aacctgccgc agcagaacct
     6061 aaagccgagg aaaaagctgc acctgctgcc aaagcggacg gcaaataggt ttatgaaacc
     6121 gtttgtgccg cctgccatgg caatgcgatt ccgggtattc cccatgtagg catcaaagcc
     6181 gattgggccg accgcatcaa aaaaggcaag gacacgttgc acaaacacgc gatcgaaggt
     6241 ttcaatacga tgcccgcaaa aggcggtcgc ggcgatttga gcgatgatga ggttaaagct
     6301 gcggttgact atatggtcaa ccagtcaggc ggcaaattct aacttgactg agtttccgat
     6361 attatcggtt atgaaaatcc agattcagta ttgaatctgg attttttgtt ttaacgggca
     6421 tccggtttta aacgcagact gcctatttgt tgtaatgaat tttgacttta gtcataaaga
     6481 attagtcttt attgtttata atcgcgcatc atcacgatag ccctattttc aaaaggacga
     6541 tttatggata cacaaatcaa gactgaagcc gacaatcaga gcaaccggcg ttatctgacc
     6601 gtttggcggt ggcattttta tgccggtctg ctggttatgc cttttcttac cctgcttgcc
     6661 gttacgggtt tgggtatgct gctgtttgcc aatattaccg gtcaggaggg cgagcggatt
     6721 catgttgtgc cgcaggcaac ggtacaacct ctgtctgttc aggcggaagc ggcacgcagt
     6781 gccgttaatc cggaaacttc gtccgtcgtt cagtatattg cgccgcgcgc cgaggacatg
     6841 gttgccgtgt tccgtgtcaa caatgagggt aaggcaacga tggtcgcggt cgatccttat
     6901 acggcaaaag tagtcagcac tatgccgcgc aatcagggtt ggtatcacac gatggatgaa
     6961 atccacagcg atatgatgct cgattcgaca ggcgattatc ttttggaaac ggctgcatcg
     7021 ctgacgatta tcatgataat cagcggtttg tatctttggt gggtgaaacg gcgcggcatc
     7081 caggcgatgc tgctgccgcc aaaaggcagg gcgcgttctt ggtggcggaa tctgcacggc
     7141 gcgtttggaa cttgggtgtc gttgatttta ctgttgttct gcctgtcggg tattgcttgg
     7201 gcaggtattt ggggcggcaa gttcgtgcag gcttggagtc agttcccggc aggcaaatgg
     7261 ggtgtcgaac cgaaccctgt ttcagtcgtg ccgacccacg gcgaggtatt gaatgacggc
     7321 aaggttaagg aagtgccgtg ggttttggag cttacgccta tgcctgtttc agggacgact
     7381 gtgggcaaag acggtattaa ccctgacgag ccgatgacat tggaaaccgt cgaccgtttt
     7441 gcgcgggaaa tcggtttcaa agggcgttat cagctgaatt tgcccaaagg cgaggacggc
     7501 gtatggactt tgtcgcagga ttctatgagt tacgacatga tcagcccgtt tgctgaccgc
     7561 acggtgcata tcgaccagta cagcggcaag attcttgccg acatccgttt tgatgattac
     7621 aacccgttcg gcaaatttat ggcggcgagc attgcgctgc atatggggac tttgggctgg
     7681 tggagcgtgt tggcgaacgt tttgttctgc cttgccgtca tttttatcgg tatcagcggt
     7741 tgcgtgatgt ggtggaaacg ccgtccgtcc ggcgcggtgg gcatcgttcc gccggcgcag
     7801 aaagtcaagc tgccggtttg gtggatgatg gcattgccgc tattggcaat cgcactgctc
     7861 ttcccgaccg cactgcttgc cattgccgtg atttggctgt tggatacctt gctgctgtcg
     7921 cggattcctg ttttgaggag atggtttaaa tgacccgatg ccgtctgaaa ggttcagacg
     7981 gcattttgtt tgaaggcagg cgggggaaag gctatataat cccgaatact tttccgcaac
     8041 ttttgtcgaa atcatgtttt atctgtatca atccaaccgt cttgaaacgc tggcggcact
     8101 gtttgcccac attcaaaaag tcaaaccgct gaaatcggct ttacagcccg aacagattat
     8161 tgtgcagagt caggggatgc gccgctacct caacacctgc ctcgcccgcg atttgggcgt
     8221 ggcggcgaat ttggcgttca gcctgcccgc cagcctgacg tggaagctga tgaaaaaact
     8281 gattcccggc attccggaac tcagcccgtt tgcacccgaa gtcatgcgct ggcggctgct
     8341 ggatttgttc cgcagcgaag cattccggaa tacggcagaa tttgaagatg tgaggaatgt
     8401 gctgcaagac tatctaggca gcggcgaatc ggcagattac cagcttgcgg gacagcttgc
     8461 ggacatattc gaccaatacc ttgtctatcg tccgcagtgg atagacgcgt ggcagcaggg
     8521 caggatactc ggtttgggcg acgatgagat ctggcagtcc aaactgtggc gttacctcga
     8581 cgacggcagg cagagcgcgc cgcaccgtgt cgcgttgtgg gaaaaactgc ttgccgcgtt
     8641 ggacaaggat aagctgcccg agcgttattt cgtgttcggc atttccacga tggcgccgat
     8701 gtatcttcag cttttgcaca agctgtccga acattgcgat gtgttcgtgt tcgcactcaa
     8761 tccgagcggg atgcattggg gcaatgtcat cgaggcggcg caaatcctca aaggtggcgg
     8821 cgatcccgat ttaactcagg cagggcatcc gctgctcgct tcattgggca agcaggggcg
     8881 ggactttttc gactttttga acgaaatgga aatagaaggc gaaacgccgg tatttgaaga
     8941 aggcggacgc gatacgcttt tgcacgccct gcaaaccgat attcaaaacc tgaaaatgcc
     9001 gtctgaaaat gtgggaagcg tcaacacggg cgacggctcg atacgcatcg tctccgcaca
     9061 cagccccttg cgcgaattgc agatactcaa agacaagctg ttgaaaattc tgcatgaaca
     9121 tcccgattgg cagccgcatg atatcgccgt attaaccccg aacatcgaat cctatacgcc
     9181 ttttatcgaa gccgtgttcg gacaggcgca gcccggtgcg caggcattgc cgtattccgt
     9241 ttcagacgtg aaaatcagcc gccgacaacc gtttttttat acattggaat gcctgtccga
     9301 cttgttggaa agccgatttg aagtcgataa agtgctcgcg cttttggaaa ccgcgcccgt
     9361 attgcgccgt ttcggactga ctgaggacga tttgccgctt ttgcacgata tggttgccga
     9421 tttgaacgtt cactggggtt tggacggaga aatgcgcggc ggcacggatc agctgttcac
     9481 ctggaagcag gcggtagaac gcatgatatt gggctggatg ctgcccgaag gcggcaaccc
     9541 gatgtggcag gatgtcagcg cgtggtatgc cgacgtgaac caaaccgccc tgttcggacg
     9601 ttttgccgcc ttcctcgaaa ccctgacgga tattgtacgg atatggcggc agcccgcaac
     9661 ggttgatgaa tgggtaggac gttgccgagg tttgcttaaa acattgttcc aaaccgaagc
     9721 cgatgaccaa aaggcagtcc aaaacctcga aaacgaatgg gtcaaatggc aggcggaaac
     9781 cgaattggcg caattttccg gacagttgcc gccgcacacc gtcatccgcc acatccgccg
     9841 ctttctcgac agcgaaagcg aggcaggctt tttacgcggc ggcatcacct tttgcagtat
     9901 ggtgccgatg cggagcctgc cctttaaagt catctgtctg ttgggtttga acgacggaga
     9961 ttttccccgc aataccaaag ccgccgtatt cgacctgatt gccaaacatc ccgccaaagg
    10021 cgaccgcgcc cgacgcgatg acgaccgcta cctgttcctt gaagctctca tcagcgcgcg
    10081 cgaaatcctg tatttgtcct acatcgggcg cgacatccgc aaagacgaag agcttgcccc
    10141 gtcttcactg ttgggcgaac tcatcgacac agttgccgcc atgaccggca caaacagccg
    10201 ccaacttgca caaaattgga tagaacagca tccgctgcaa gccttctcgc gccgatattt
    10261 ccaagaaggc ggacgttcag acggcatatt cggcacgcgt accgactacg ccgccgcgct
    10321 tggacaaatg cccgaaccgc cacaaccctt tttcgaccaa tccgtagaaa acgccgaacc
    10381 cgttgccgaa atcggacagg acgaatttat ccgcttctgg cgcaaccccg tcaaagcatg
    10441 gcttcagcag cagcttgcgt ggagcgaacc ccatatcggc gaagcttggg agcctgccga
    10501 acccttcgag ccgcaacacg ccgatcaaat cgccgaaacc tacatcgaag cacggcgcga
    10561 aggacgggat tttgcccaaa ccgctgcccg catcggggcg gaaagcctgc tgccgtcggg
    10621 agagttgggc agactttggc agcagtcttt ccaacttgcc gccaaacaaa tcgacacggc
    10681 tattttaaac agccccaaac tgccaccgct ttcatacgcc ataccgtcgg acgggcaaat
    10741 cctgaaaggc agtttgagca atttatacca atgcggacaa gtgttttacg cctacggcaa
    10801 acccaatgcg ccgcaacgtg tggctttttt gttggaacac ctgatatttt gcgccgttat
    10861 gccgtctgaa gccgaaacgc ggcaaacctt tatcatccaa tccggagaaa ccgaagtatt
    10921 ggcggaaatc gcgcaaggca gggcattgcg gctattgtcg gaatggatgg cgtttttcaa
    10981 tatcgggcaa aaccgcccgc tgccgttttt tgccaaaacc tcgcttgcca ccgccgaagc
    11041 gtttgtccaa aaacaagatt gggaagccgc cctgaaaaaa gcccaaaccg cctaccacgg
    11101 caacaaagtc agcaaagggc agaaagacta taccgaagtc gcccttgtgt ttggcaatgc
    11161 aagccaaaac ccgctcgaac agcccctgtt tgaaaacctc gtccgcctgc ttgccgacac
    11221 gcttgccgca gcggaaaaaa aggaagaggc cggagcagca tagaaaaagc ataacaggat
    11281 agaaagacaa atgccgtctg aaagcgttta agcatttcag acggcatttt taccaaggct
    11341 tgattatttc cggcgcaggc agggatggct ttccaagttg tccatcatta tcccgatgat
    11401 gcgtggggcg gttttgccca aatcgaaatt ttctccggaa gagagccaac gccaaatcag
    11461 cccgtccaag gtcgatttga tgaagatgac cgccgtttct ttgtccaaat cgtctgccaa
    11521 atcctgattt tccaccgctt cggtcaaaac ggcggtaatt ttttcgcgcc agattgcctg
    11581 atgcttgcgg gcaatggcga taacggcggc gttttgctcc gtgtgttcgc attttaaaaa
    11641 caggatgttg tggaatttgt agtggatgtc gttgctttgc agccgctcga aaaagtgcag
    11701 cacgtgtggc ggaataccgt ccaagaccct ccttcggcat ttgcggcatc ttgcgcgatg
    11761 cagttttcga tgtcgtcgca gatacgttgg aacagcgcgt cgaacaagtc ttccttattt
    11821 ttgaaatgcc aatagagcgc gccgcgcgtt acgccggcgg cttgggcgat ttcgttgagc
    11881 gaggtgcgcg caatcccttt gcggtaaaag gtttccaagg cggcaagcat caggtgttct
    11941 ttggttttca aggcttcggt tttggttttt ctcataatgg cgttttcgtt ccgtcgcggt
    12001 ttgacggttg ggattataaa aaagactttg tatccgtgca atcgtgtatg tataataacg
    12061 cccctgaaat tgagacagtt tcccagtgcc gaagtgccga acccacctgc tttgcaaccc
    12121 gtttgaatac agggatgccg aagcaggcgg ttttgtattt tgaaacggga atttctgtta
    12181 aatctaaaat cctttaataa aatcatacag ataggataga taatggcttt ttatgcttct
    12241 aaggcgatgc gtgcggccgc gttggctgcc tctgtcgcgt tggcactgtc atcttgcggc
    12301 aaagcagatg aagcggcaca gggcgggcag cctgctggtc gggaagctcc ggctcccgtc
    12361 gtcggcgttg ttaccgtcca tccgcaaacc gttgcgttga ccgtcgagtt gccgggacgt
    12421 ttggaatcgc tacgtaccgc cgatgtccgt gcgcaagttg gcggcattat ccaaaaacgc
    12481 ctgttccaag aaggcagtta tgtccgtgcc ggacagccgc tgtatcagat cgacagttcc
    12541 acttatgaag caagtctgga aagcgcgcgc gcgcaactgg caacggcaca ggcaacgctt
    12601 gccaaagcgg atgcggattt ggcgcgttac aaaccgctgg tttccgccga tgccatcagt
    12661 aaacaagagt acgatgctgc ggtaacggcg aaacgttctg ccgaggcagg cgtcaaagcg
    12721 gcacaggcgg ccatcaaatc cgccggcatc aatctgaacc gttcgcgcat tactgcgccg
    12781 atttccggct ttatcggtca gtccaaagtt tccgaaggta cgctgttaaa tgcgggcgat
    12841 acgactgttt tagccaccat ccgccaaacc aatccgatgt atgtgaacgt tacccagtct
    12901 gcatccgaag tgatgaaact gcgccaacag gttgccgagg gcaagctact ggcggcggat
    12961 ggtgcgattg cggtcggcat caaatttgac gacggcacgg tttaccctga aaaaggccgc
    13021 ctgctgtttg ccgatccaac cgtcaacgaa tcgactggtc agattaccct gcgcgccgcc
    13081 gtaccgaacg atcagaacat cctgatgccc ggcctgtatg tgcgcgtgct gatggatcaa
    13141 gtggcggcgg acaacgcatt tgttgtgccg cagcaggcgg taacgcgcgg tgcgaaagat
    13201 accgtgatga ttgtgaacgc ccaaggcggt atggaacccc gcgaggtaac ggttgcacaa
    13261 cagcagggcg cgaattggat tgttacgtcg ggtctgaagg acggggacaa ggtggttgtg
    13321 gaaggcatca gtatcgccgg tatgacgggg gcgaaaaagg taacgcctaa agaatggatg
    13381 ccgtctgaaa accaagccgc cgcccctcaa gccgacgttc agacggcatc tgaagccaaa
    13441 cctgcttctg aagcgaaata aggaaggcat cgatggctaa attttttatc gaccgcccca
    13501 tttttgcgtg ggttatttcg attttcatta ttgcggcggg tattttcggc attaaaagct
    13561 tgcctgtttc gcaatatccg tctgttgccg caccgaatat taccctgagg gctacttatc
    13621 cgggtgcgtc cgcgcaggta atggaagaca gcgtgctttc cgtgatcgaa cgcaatatga
    13681 acggcgtgga aggtttggac tatatgacca cttccgccga ttcgagcggc agcggcagcg
    13741 tgagcctgac ctttacgcct gaaaccgacg aagatttggc tcaggtggag gtgcaaaaca
    13801 agctttccga agtattgagc atgttgccgg caaccgttca acagtacggc gttaccgtat
    13861 ccaaagcgcg ttccaatttc ttgatgattg tgatgctgtc gtcagacgta cagtcaaccg
    13921 aggagatgaa cgactacgcg cagcgcaata ttgtgcccga actgcaacgt atcgacgggg
    13981 tgggcaaggt tcagttgttc ggcgcgcaac gcgccatgcg gatttgggtc gatcctaaga
    14041 aactgcaaaa ctacaatttg tcgtttgctg atgtcggcag tgcgctgtcg gcacagaata
    14101 ttcaaatttc cgcaggttcc atcggttcgc ttcccgctgt tcaggggcag actgttacgg
    14161 ctaccgtaac ggcgcaaggg cagttgagta cggcagaaga attcggcaac gtcatcctcc
    14221 gcgccaatac tgatggttct aatgtttacc tgaaggatgt agcgaaagtc ggattgggta
    14281 tggaagacta ttcttcctca acccgtctga acggggtaaa taccaccggt atggcggtga
    14341 tgctgtccaa tagcggcaat gcgatggcga cggcaaaggc ggttaaagaa cgcatggcga
    14401 cgttggaaaa atactttcct caaggtatga gctggaaaac cccttacgat acttccaaat
    14461 tcgtcgaaat ttcgattgaa aaagtgattc acactttaat cgaagcgatg gtgctggtgt
    14521 ttgtcgtaat gtacctcttc ctgcaaaaca tccgttatac gctgattccg accatcgtcg
    14581 taccgatttc cctgttgggt ggtttcgcct tcatctctta tatggggatg tcgattaacg
    14641 tactgaccat gtttgcgatg gtattggtca tcggtatcgt ggtcgatgac gcgattgtgg
    14701 tggttgaaaa cgtcgagcgc attatggcgg gcgaaggcct gccgcctaag gctgcaacca
    14761 aaaaagcaat gggtcagatt tccggtgcgg taatcggtat tactgctgtt ttgatgtctg
    14821 tattcgttcc tcttgctatg ttcagcgggg cgacgggcaa tatctataaa cagtttgcac
    14881 tgactatggc ggcatcgata gccttctccg ccttccttgc gctgacactc acacctgctt
    14941 tgtgtgccac aatgttgaag gtaatcccga aagggcatca cgaagagaaa aaaggtttct
    15001 tcggttggtt caacaaaaaa ttcgacaact ggacacacgg ctacgaaggc tgggttgcca
    15061 aagtgttgcg taagactttc cgcatgatgg ttatttacgt tggtttggcg gttgtgggcg
    15121 tattcctgtt tatgcgccta ccgacttctt tcttgccgac cgaagaccaa ggcttcgtca
    15181 tggtcagcgt gcaactgcct gcgggggcga ccaaagaacg caccgatgcg actttggcac
    15241 aagttaccca attggcgaaa agcattcccg aaatcgaaaa catcatcacc gtttccggct
    15301 tcagcttttc gggcagcggt cagaatatgg cgatgggttt tgcgatgctt aaggattgga
    15361 acgaacgtac cgccccgggc agtgatgccg tttcgattgc cggcaagctg acgggtatga
    15421 tgatggggac gctgaaagac ggtttcggca tcgccatcgt ccctcctccg attatggagt
    15481 tgggcaacgg ttcgggcctg accatcaacc tgcaagaccg caacaacacc ggccataccg
    15541 cattgctggc gaagcgcaac gagttgattc aaaaaatgcg ggccagcggt ttgtttgacc
    15601 cgagtaccgt ccgtgctggc ggtttggaag atgcgccgca gttgaaaatc gacatcaacc
    15661 gtgccgccgc agccgcacaa ggcatttcgt ttgccgacat ccgcaccgca ttggcaagcg
    15721 cgctgagttc gtcttatgtc aacgacttcc cgaaccaagg ccgtctgcag cgcgtgatgg
    15781 tgcaggcaga cggggatgcc cgtatgcagc ctgcagatat tttgaacctg accgtgccga
    15841 acaaatccgg cgttgccgta ccgctttcca ccattgctac tgtttcttgg gaaaacggta
    15901 cggaacagag cgtgcgcttc aacggttatc ccgctatgga tctgtccgct tcgcccgcaa
    15961 ccggcgtttc caccgggcag gctatggcgg cggttcaaaa aatggttgac gaaatgggcg
    16021 gcggttacag cctggagtgg ggcggacagt cgcgcgaaga ggcaaaaggc ggctcgcaaa
    16081 ccattgccct ttacgcgctt gccgccgttg cagtcttctt ggtgcttgcc gcactttatg
    16141 aaagctggtc tattccgctg gccgttctgc ttgtgatgcc tttaggcttg gcaggggcgg
    16201 cggcaggtgt aaccgggcgc aacctgaccg agctgatgtt gggcggtatt cctgcatttg
    16261 ccaacgacat ctacttccaa gtcggtttcg ttaccgtgat gggcttgagt gcgaaaaacg
    16321 cgattctgat tatcgagttt gccaaagacc ttcaagcgca agggaaaagc gcggtcgaag
    16381 ccgctttgga agccgcccgc ctgcgtttcc gtccgatcat catgacctcg ttcgccttta
    16441 ttttgggcgt ggttcccctg tatattgccg gcggtgcgag ttctgccagc cagcgcgcca
    16501 tcggcacgac cgtgttctgg gggatgctgg tcggtacgct cttgtccgtg ttccttgttc
    16561 cgcttttcta tgtggtggtg cgcaaattct tcaaagaaac tgcacacgaa cacgaaatgg
    16621 cggtgaaaca cgctgccgaa gcgggcatca ccggttcgga cgacagccaa cattaagcaa
    16681 ccatgccgtc tgaacgccca cgggttttca gacggcatca ggactttctt atgaatacta
    16741 cattgaaaac taccttgact tctgttgcag cctttgcatt atccgcctgc acgatgattc
    16801 cccaatacga acggcccaaa gtcgaagttg cggaaacgtt taaaaacgat actgccgaca
    16861 gcggcatccg cgccgtcgat ttaggttggc atgactattt tgccgacccg cgcctgcaaa
    16921 agctgattga catcgcactt gagcgcaata ccagtttgcg taccgccgta ttgaacagcg
    16981 aaatctaccg caaacaatat atgatagagc gcaacaacct cctgcccacg cttgccgcca
    17041 atgcgaacgg ctcgcgtcaa ggcagtttga gcggcggcaa tgtcagcagc agctacaatg
    17101 tcggactggg tgcggcatct tacgaactcg acctgttcgg acgcgtccgc agcagcagcg
    17161 aggcggcact gcaaggctat tttgccagca ccgccaaccg cgatgcggca catttgagcc
    17221 tgattgccac cgttgccaaa gcctatttca acgaacgtta tgccgaagaa gcgatgtctt
    17281 tggcgcagcg tgttttgaaa acgcgcgagg aaacctacaa gctgtccgaa ttgcgttaca
    17341 aggcaggcgt gatttccgcc gtcgccctgc gccagcagga agccttgatt gaatctgcca
    17401 aagccgatta tgcccatgcc gcgcgcagcc gcgagcaggc gcgcaatgcc ttggcaacct
    17461 tgattaacca accgataccc gacgacctgc ccgccggttt gccgttggac aagcagtttt
    17521 ttgttgaaaa actgccggcc ggtttgagtt ccgaagtatt gctcgaccgt cccgacatcc
    17581 gtgccgccga acacgcgctc aaacaggcaa acgccaatat cggtgcggca cgcgccgcct
    17641 ttttcccatc catccgcctg actggaagcg tcggtacggg ttctgccgaa ttgggcgggt
    17701 tgttcaaaag cggcaccggc gtttggtcgt ttgcgccgtc tattaccctg ccgattttta
    17761 cctggggtac gaacaaagcc aaccttgatg cagccaagct gcgccaacag gcacaaatcg
    17821 ttgcctatga agccgccgtc caatccgcct ttcaagacgt ggcaaacgca ttggcggcgc
    17881 gcgagcagct ggataaagcc tatgccgctt taagcaaaca aagccgcgcc tctaaagaag
    17941 cgttgcgctt ggtcggcctg cgttacaagc acggcgtatc cggcgcgctc gacttgctcg
    18001 atgcggaacg cagcagctat tcggcggaag gtgcggcttt gtcggcacaa ctgacccgtg
    18061 ccgaaaacct tgccgatttg tacaaggcgc tcggcggcgg attgaaacgg gatacccaaa
    18121 ccgacaaata accgcccggg caataaaatg ccgtctgaaa cgataaggtt tcagacggca
    18181 tttctttct
//
LOCUS       DAXIEB010000033        17889 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000033 DAXIEB010000000
VERSION     DAXIEB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.033"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(87..1370)
                     /gene="hemL"
                     /locus_tag="ACFOA3_000911"
     CDS             complement(87..1370)
                     /gene="hemL"
                     /locus_tag="ACFOA3_000911"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6897937.1"
                     /translation="MNRNEILFDRAKATIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPVLIDETVAVAVEVFKAMAA"
     gene            complement(1434..1817)
                     /locus_tag="ACFOA3_000912"
     CDS             complement(1434..1817)
                     /locus_tag="ACFOA3_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6897938.1"
                     /translation="MYCLRLRRLVLIFVNPLYQTEEGIAQTVVKGKTTRAEVEARFGK
                     RNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDGEGVVRDYRFTHKVEKD
                     ERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            complement(1920..2000)
                     /locus_tag="ACFOA3_000913"
                     /pseudo
     CDS             complement(1920..2000)
                     /locus_tag="ACFOA3_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2203..3531
                     /gene="miaB"
                     /locus_tag="ACFOA3_000914"
     CDS             2203..3531
                     /gene="miaB"
                     /locus_tag="ACFOA3_000914"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6897939.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIVKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(3616..5529)
                     /gene="dxs"
                     /locus_tag="ACFOA3_000915"
     CDS             complement(3616..5529)
                     /gene="dxs"
                     /locus_tag="ACFOA3_000915"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6897940.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(5614..6531)
                     /gene="xerC"
                     /locus_tag="ACFOA3_000916"
     CDS             complement(5614..6531)
                     /gene="xerC"
                     /locus_tag="ACFOA3_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6897941.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6725..7789
                     /gene="fba"
                     /locus_tag="ACFOA3_000917"
     CDS             6725..7789
                     /gene="fba"
                     /locus_tag="ACFOA3_000917"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6897942.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            7947..8768
                     /locus_tag="ACFOA3_000918"
     CDS             7947..8768
                     /locus_tag="ACFOA3_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6897943.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPGGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKA
                     DEKSHAVILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            8907..9584
                     /gene="tsaB"
                     /locus_tag="ACFOA3_000919"
     CDS             8907..9584
                     /gene="tsaB"
                     /locus_tag="ACFOA3_000919"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6897944.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASMPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            9581..10021
                     /gene="rimI"
                     /locus_tag="ACFOA3_000920"
     CDS             9581..10021
                     /gene="rimI"
                     /locus_tag="ACFOA3_000920"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6897945.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            10015..10749
                     /locus_tag="ACFOA3_000921"
     CDS             10015..10749
                     /locus_tag="ACFOA3_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6897946.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNTLKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIARELDSCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARTWQVLKQLKHALQAGGS"
     gene            10811..11452
                     /gene="pyrE"
                     /locus_tag="ACFOA3_000922"
     CDS             10811..11452
                     /gene="pyrE"
                     /locus_tag="ACFOA3_000922"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6897947.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            11526..11960
                     /locus_tag="ACFOA3_000923"
     CDS             11526..11960
                     /locus_tag="ACFOA3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6897948.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            11957..13267
                     /gene="argA"
                     /locus_tag="ACFOA3_000924"
     CDS             11957..13267
                     /gene="argA"
                     /locus_tag="ACFOA3_000924"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6897949.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTLHLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPEARRKAYRNNGRNSHILVRRLHR"
     gene            13363..15378
                     /locus_tag="ACFOA3_000925"
     CDS             13363..15378
                     /locus_tag="ACFOA3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6897950.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSLPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(15492..16448)
                     /locus_tag="ACFOA3_000926"
     CDS             complement(15492..16448)
                     /locus_tag="ACFOA3_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6897951.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            16684..17649
                     /locus_tag="ACFOA3_000927"
     CDS             16684..17649
                     /locus_tag="ACFOA3_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6897952.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGSARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT         3992 a         5419 c         4878 g         3600 t
ORIGIN      
        1 cccgcctgcg tgggaatgac ggcggtgtgc attcttactt caatctgcca aacaaatcga
       61 acagagaaac cctgtccgtc aaaacatcat gcagccatcg ccttgaacac ttcaaccgca
      121 accgcaaccg tttcatcaat caaaaccggc gtatgcgcgg cggaaacgaa acctgcttca
      181 taagcggacg ggccgaaggc gacattgcgg tcgagcatcc cgtggaaaaa ctgtttgaag
      241 ccttcaatat tggaacgcgc catatcggca taatttcgcg gcgcgtgtgc ggcgaaatac
      301 agaccgaaca taccgcccac gctgtcggcg gtgaactcga tgcccgccgc atccgctgcc
      361 gtctgaaaac cttgaaccaa ctgttcggta cgcgccgtca ggttttcata aaacccttcg
      421 cgctgaatga tttccagcgt tttcaagcct gcggcgacgg caatcgggtt gcccgacaag
      481 gtgcctgcct gatacacgcc gcccagcggg gaaatacatt ccatgatgtc tttgcgcccg
      541 ccgaacgcgg caagcggcat accgccgccg atgactttgc ccatcgtggt caggtcgggc
      601 atgatgccgt gcagcgattg tgcgccgccg agcgcgacgc ggaaaccggt catcacttcg
      661 tcgtaaatca acaccgcgcc gtgtttttcg gtcaattcgc gcagggcttt gacgaaggct
      721 tcggtcgggc ggacaaggtt catattgccg acaaaaggct ccaaaatcac acaggcaatg
      781 tcattgccgc tttgagcaaa ggcttcttcc aattgggcga tgttgttgta ctcaaggact
      841 aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag aaggattgcc gaacgtcagc
      901 aggccgctgc cggctttcac cagcaggctg tcggaatgcc cgtggtagca gccttcaaac
      961 ttgatgattt tgtcgcgacc ggtaaaaccg cgtgccaaac ggatggcggt catcgtcgct
     1021 tcggtaccgg agctgacgag gcgcagccgt tcgacggacg gcatgatttt ggcgatttgt
     1081 tcggcaatgg cgatttcgcc ctcggtgggc gcgccgaacg acaaaccgcc caacgcggct
     1141 tcgcgcacgg cttcgacgac ttcggggtgc gcatgtccga caatcgcagg cccccaagag
     1201 ccgacatagt cggtataacg cgtgccgttt tcgtcccaaa catacgcgcc ttcggctttt
     1261 ttgatgaagc gcggcacgcc gccgacgctg ccgaatgcgc ggacagggga attcacgccg
     1321 ccggggatgg tggctttggc gcggtcgaat aaaatttcgt tacggttcat atttatcctt
     1381 aaattccatc ctaaaggcag gttttaggtt ctgaaacaga aaaagctatt ttatcatttt
     1441 tcaggttgcg acaaggattt gcccgcttct ttgcggatca cgccaaccgc atcccggatg
     1501 acggaacgct cgtctttttc cactttatgt gtaaagcggt agtcccggac gactccctcc
     1561 ccgtcgtaat ccacacacca ctcccaatgc cggcgttctg atttcatata aatgaaattg
     1621 gtcggcaaaa aattataaat cggcaggctg acctcgtgat aggcataaca accgaaaggg
     1681 ttgcgcttcc cgaaacgtgc ctctacctcc gcccgggtcg ttttgccttt aacaaccgtt
     1741 tgtgcgattc cctcttccgt ctgatatagt ggattaacaa aaatcaggac aaggcgacga
     1801 agccgcagac agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga
     1861 atcgttctct ttgagctaag gcgaggcaac gctgtactgg tttttgttaa tccactataa
     1921 cgcaggaact gatgttccct gtcgccaaaa ttgctggtac acgcacacag cagcaatgcc
     1981 gcccatacaa ccggtttcat acacatctcc cattaaagcc aaacattata cagccgtcca
     2041 aaccgattaa attcatattt taaaacaata tcctgcctcc aaaacccaca tcgtgctata
     2101 atccacaccg attttcagac ggcatcgtca tgccgtctga aattttttca ttccaacaac
     2161 aatcagcccc gcgattacgg ctgcctgaga aagacacaaa ccatgaaaaa agtatttatc
     2221 cgcaccttcg gctgccagat gaacgaatac gacagcgaca aaatgctcgc cgtcctcgcc
     2281 gaagaacacg gcggcatcga acaggttacc caagccgacg aagccgacat catcttgttc
     2341 aacacctgct ccgtgcgcga aaaagcgcaa gaaaaagtct tctccgattt ggggcgcgtg
     2401 cgtccgctca aagaaaaaaa ccccggcctc atcatcggcg ttgccggttg cgtcgcctcg
     2461 caagaaggcg aaaacatcgt caaacgcgcg ccgtatgtgg acgtggtttt cggcccgcaa
     2521 acgctgcacc gcctgcccaa aatgattgtg gacaaagaaa ccagcgggct gtcgcaagtc
     2581 gatatttcct ttcccgaaat cgaaaaattc gaccacctgc cgcccgcccg cgtcgaaggc
     2641 ggcgcggcat ttgtatcgat tatggaaggc tgttccaaat actgctcctt ctgcgtcgtc
     2701 ccctacacgc gcggcgaaga attctcccgc ccgctcaacg acgtattgac agaaatcgcc
     2761 aaccttgccc agcaaggcgt gaaagaaatc aacctcttgg gacaaaacgt caacgcctat
     2821 tgcggcgaaa tggacgacgg cgaaatctgc gacttcgcca ccctgctgcg catcgtccac
     2881 gaaatccccg gcatcgaacg tatgcgcttc accaccagcc acccgcgcga gtttaccgac
     2941 tcgattatcg agtgctaccg cgacctgccc aaattggttt cccacctgca cctgccgatt
     3001 caaagcggtt ccgaccgcgt attgagcgcg atgaaacgcg gctacaccgc tttggaatac
     3061 aaatccatca tccgcaaact gcgtgccatc cgtcctgatt tatgcctgag cagcgatttc
     3121 atcgtcggct tccccggcga gaccgaacgc gagttcgagc aaaccttgaa actggtgaaa
     3181 gacattgcct tcgacttgag cttcgtgttt atttacagtc cgcgccccgg cacgcctgcc
     3241 gccaacctgc acgacgacac gccgcacgaa gaaaaagtgc gccgcctcga agccttgaac
     3301 gaagtcatcg aagccgaaac cgcgcgcatc aaccaaacga tgatcggcac ggttcaacgc
     3361 tgcctggtcg aaggcatctc caaaaaagac cccgaccagc tccaagcccg caccgccaac
     3421 aaccgcgtcg tcaactttac cggcacgccc aacatgatta accaaatgat cgatttggaa
     3481 atcaccgagg cctacacctt ctccctgcgc ggcaaagttg tcgaagccta aacccttacg
     3541 ccgaaaaaat gccgtctgaa gcgtttcaga cggcattttg ccttgtatcg gcagacgatg
     3601 gcgcggccag gcggcttaat ttgccgcatc ccgatccgac aaccacgcgc gcacacgccg
     3661 ttccaccgct tcggcactca agcccaaatc gtctaaaagt tttttcggat cgccgtgtcc
     3721 ggttacggta tcggcaacgc ccaaaagcaa aacgggtttg cagatgccgt gtttcgccaa
     3781 tacttccagc accgcgccgc ctgcgccgcc ctgttcggcg ttttcttcaa gggtaacgat
     3841 gcggtcgtgg cttcgggcaa ggcggacaat caactcttcg tctatcggtt tgacgaagcg
     3901 catatcggcg acggtggcgt tcagtttttc ggcaaccgcc aatgcggggg cgaccatact
     3961 gccgaaggca atgaatgcgg ttttctcacc ttcgcggcgg ataatgccct tgccgatttc
     4021 cacggtttcc atgccgtctg aaaccggcgc gcccgtaccc gtgccgcgcg gatagcggac
     4081 ggcggcgggc gcgtctgcct gatagcaggt cgaaagcaac aggcggcatt cgttttcatc
     4141 gctcggcgcg gcgacaatca tgttcggcac gcagcgcaaa aagctcaaat cgtacagacc
     4201 ggcatgggtc gggccgtccg cgccgacgat gcccgcgcgg tcgacggcaa acaaaacggg
     4261 taggttttgc agggcgatgt cgtgcaccag ttggtcgtag gcgcgttgta aaaaggtgga
     4321 ataaatcgcc acgacgggct tcatgccttc gcacgccaaa ccgccggcaa aggtaacggc
     4381 gtgctgctcg gcaatgccga catcgaaata acggtcgggg aatcgttgtt caaactcaac
     4441 caagccgctg ccctcgcgca tcgcgggcgt gatggcgatc aaacgctcgt ctgccgccgc
     4501 ccggtcgcac agccatttgc cgaacacttg ggtataggtc ggtttggcga cgggcttggg
     4561 ttctttttca gacggcattt gcgccgcgcc ttctttaggc agcttggcga cggcgtggta
     4621 tttgacgggg tcgttttcgg cgagtttgta gccgttgcct tttttggtga tgacgtgcag
     4681 aagctgaggg cctttgcggc tgcgcaagtc tttcaatacg tccaccagat tttcgacgtt
     4741 gtgtccgtcc acggggccgg tgtagcggaa gccgaagttt tcaaacaaag acagcgactg
     4801 tttggcgtgt tcggcttctt cggcaagggt tttgattttg tgttcgactt tttgggcaaa
     4861 ctccatcgcg ccgggtattt tgtctaatac cttgcccgtt tgcgctttga cggtactcaa
     4921 caggccgtgc atatcgcgca cgacgttgct ggcaaggtat ttcggcagcg cgccgacgtt
     4981 gggggaaatc gacatttcgt tgtcgttgag gacgaccagc aaatccacat ccatatcgcc
     5041 tgcgcaattc aaggcttcaa acgcctgccc cgccgtcatc gcgccgtcgc cgatgatggc
     5101 gacgctgcgg cggtcgctgc ccaagagttt gtctgccgcc gccatgccca acgccgcgcc
     5161 gatggatgtg gaagaatgcc ctacgccgaa cgcgtcgtat tccgactcgc tgcgttttgg
     5221 gaagccagct aaaccgccgt attggcgcat ggtgtgcatc tggtttttgc gtccggtaag
     5281 gattttgtgc ggatagcttt ggtgtccgac atcccacacc agcttgtctt cgggcgtgtt
     5341 gtacacatag tgcagggcga tggtcagctc gaccgcacct aagttgctgg cgaaatgacc
     5401 gccggtttga ccgacggatt ccagcagaaa ggcgcgcaac tcgccggcaa ggcgcggcag
     5461 ctgttttttg tccagacggc gcaaatcttg cgggctgtca atcaggtcga gtagggggct
     5521 tgggttcatg gtgtgtcttt tttatgtgtc gtccgggtgc aacggccaat tatatatcaa
     5581 gagtgtgcgg ctgacggctg attttgccgt atgtcattcg tcctgccgct tggcgcgcgg
     5641 gtgggcttcg tcatacaggc gggcgatgtg gtcgaaatcg agcttggtat aaatctgcgt
     5701 ggtcgaaagg ctgctgtgcc cgagcagctc ctgcaccgcc ctgatgtcgc gcgaagcctg
     5761 caataggtgt ccggcgtagc tgtggcgcat catatgcggc gaaacgtgcc tgccgtcgcc
     5821 gttttgcgcc gcccattgcg ccaaacgttt ttggatttgg cgttggctca ggcgcgtgcc
     5881 gttcctgccg gtaaacaggg ctttgccgtc cgatgccgtc tgacgcagcg gcagatagtt
     5941 tttcagggct tccacgcttt tgccggtcag cggcacctgc cgctgcttgc gccctttgcc
     6001 ggtaacgtgt acccacgcct cgtccaaata gacatcatct gcattcaagc cgtgtatctc
     6061 gctcacgcgc aaaccgctgc cgtacatcag ttcgaacaga gcgtggtcgc gcaccgccag
     6121 cgggtcgccg ccgtccacgg gcaaatccag catccggttc agccattcct gcggcagggc
     6181 tttgggtacg cgctcgggct gcttcggcgg tttgatgtcg gcggtcgggt cggtatgcag
     6241 cagaccgcgt tccaccagcc aaacacaata ctgccgccaa gacgacagct tgcgcgccag
     6301 cgtccgcgca ttcaaaccgc gctgcgacag ccgccgcaac gccgccgtaa aatcgcgccg
     6361 cgacaagtcc tgcggcacgc cgcctgcatc ttcagacggc atttgtgcca acagcgcaaa
     6421 cagttcttcc aaatcgcgcc ggtatgccgc caccgtgtgc tccgacttgc cctcgcgcac
     6481 gatgttttcc aaataagcgt caaaatgcgc cgcaaacccg tccaaaacca taaccgcccc
     6541 acacaaatat caaaaaaaca gtgaaaacat tatcataaac gacacaccaa aaccccgaac
     6601 cgcccaaaat ccgacccgtc cgcccgccgt tatcgttaac ataaaatcaa tgagccgttt
     6661 atggtttttt gccgcaaaaa acattataat ccgccttatt tacctattgc ccaaggagac
     6721 acaaatggca ctcgtatcca tgcgccaact gcttgatcat gctgccgaaa acagctacgg
     6781 cctgcccgcg ttcaacgtca acaacctcga acaaatgcgc gccattatgg aagccgccga
     6841 ccaagtcaac gcgcccgtca tcgtacaggc gagtgcaggt gcgcgcaaat atgcgggcgc
     6901 gccgtttttg cgccacctga ttctggcggc tgtcgaagaa tttccgcaca tccccgtcgt
     6961 gatgcaccaa gaccacggcg catcgcccga cgtgtgccaa cgctccatcc aactgggctt
     7021 ttcctccgtg atgatggacg gctcgctgat ggaagacggc aaaacccctt cttcttatga
     7081 atacaacgtc aacgccaccc gtaccgtggt taatttctcc cacgcttgcg gcgtatccgt
     7141 cgaaggcgaa atcggcgtgt tgggcaacct cgaaactggc gaagccggcg aagaagacgg
     7201 tgtaggcgca gtgggcaaac tttcccacga ccaaatgctc accagcgtcg aagatgccgt
     7261 gcgtttcgtt aaagataccg gcgttgacgc attggcgatt gccgtcggca ccagccacgg
     7321 cgcatacaaa ttcacccgtc cgcccacagg cgacgtattg cgtatcgacc gcatcaaaga
     7381 aatccaccaa gccctgccca atacacacat cgtgatgcac ggttccagct ccgttccgca
     7441 agaatggctg aaagtcatca acgaatacgg cggcaatatc ggtgaaacct acggcgtgcc
     7501 ggttgaagaa atcgtcgaag gcatcaaaca cggcgtgcgc aaagtcaaca tcgataccga
     7561 cttgcgcctt gcttccaccg gcgcggtacg ccgctacctt gccgaaaacc cgtccgactt
     7621 cgatccgcgc aaatatttga gcaaaaccat tgaagcgatg aagcaaatct gcctcgaccg
     7681 ctacctcgcg ttcggttgcg aaggtcaggc aggcaaaatc aaaccggttt ccttggaaaa
     7741 aatggcaaac cgttatgcca agggcgaatt gaaccaaatc gtcaaataac aggttgcttg
     7801 taaacaaaat gccgtctgaa ccgttgttcg gacggcattt gatttttgct tctttgacct
     7861 gcctcattga tgcagtatgc aaaaaaagat accataacca aaatgtttat atattatcta
     7921 ttctgcgtat gactaggagc aaacctgtga accgaactgc cttctgctgc ctttccctga
     7981 ccgccgccct gattctgacc gcctgcagca gcggaggcgg cggtgtcgcc gccgacatcg
     8041 gcgcggggct tgccgatgca ctaaccgcac cgctcgacca taaagacaaa agtttgcagt
     8101 ctttgacgct ggatcagtcc gtcaggaaaa acgagaaact gaagctggcg gcacaaggtg
     8161 cggaaaaaac ttatggaaac ggcgacagcc tcaatacggg caaattgaag aacgacaagg
     8221 tcagccgctt cgactttatc cgtcaaatcg aagtggacgg gcagctcatt accttggaga
     8281 gcggagagtt ccaaatatac aaacaggacc actccgccgt cgttgcccta cagattgaaa
     8341 aaatcaacaa ccccgacaaa atcgacagcc tgataaacca acgctccttc cttgtcagcg
     8401 gtttgggtgg agaacatacc gccttcaacc aactgcccgg cggcaaagcc gagtatcacg
     8461 gcaaagcatt cagctccgac gacccgaacg gcaggctgca ctactccatt gattttacca
     8521 aaaaacaggg ttacggcaga atcgaacacc tgaaaacgcc cgagcagaat gtcgagcttg
     8581 cctccgccga actcaaagca gatgaaaaat cacacgccgt cattttgggc gacacgcgct
     8641 acggcagcga agaaaaaggc acttaccacc tcgctctttt cggcgaccga gcccaagaaa
     8701 tcgccggctc ggcaaccgtg aagataaggg aaaaggttca cgaaatcggc atcgccggca
     8761 aacagtagca tttgaaaaat gccgtccgaa cacgataatt taccgttcgg acggcatttt
     8821 tgcgccatac cgccctagga tacgacggca cggcaggcaa gccggtttca ctatataatg
     8881 ccgtctgaac caacgagaac cacaccatgc aagccgattt caaccgcccc gtcctcgccg
     8941 tcgataccgg aacttcctat ttgtcgctcg cgctgcgcgc cgacggcgaa accctcctgt
     9001 tccatcagga ggccggcagc cgccagtccg aactgatcct gccggaaatc cgcaccctat
     9061 tccgaaatgc gggcattacc gccgccgatt tgggggcgat tgtttatgcg aaaggtcccg
     9121 gcgcgtttac cggactgcgt atcggcatcg gtgtggctca gggtttggca acgccgtttg
     9181 atacgccttt aatcggcatc cccacgctcg atgccgccgc ctcgatgccg ccgccgcaaa
     9241 gctgcatact cgccgctgcg gacgcgcgta tgggcgaagt gttttatgcg tggttcgaca
     9301 cgctgaaccg ccgccgtttg agcgattatc aggtcgggcg ggcggcagac atccggctgc
     9361 cggagggatg tgccttttca gacggcatag gcagcgcgtt cgcgttggaa gaaacgccgc
     9421 cgttttcagg caaacccgat atgccgaccg ccgccgactt cctcaatctg gcggcggaag
     9481 gcggttttcc cgccgtcgct gctgcacacg ccgaactgct ctacgtccgc aacaaaatcg
     9541 ccctaaccgc caaagaacag gcggaacgga aagcccgaac atgaacatcc gccgcgcctc
     9601 accgtccgac tgcgccgcac tcgccgcgct cgatgccgtc tgcaacccgt ccgcgtggac
     9661 gcaacgccaa tttgagtccg cattggtttc gccgtccgaa caggttttcc tcgcggaaaa
     9721 agacggcggg attgccgcct ttatcgtttg gcagaacctg cccgacgaat ccgaactgca
     9781 cctgattgcc accgcgcccg aatgccgccg ccgtggcgtt gcgtccgccc tgctcgaata
     9841 ttggttcgca catctgcccg aagacacgca acgcctgctg ctcgaagtcc gtgcaggcaa
     9901 caccgccgca caggcactgt acgcggcgca cggctttacc gatgcgggca ggcggaaaaa
     9961 ctattaccgt acagccgacg gtaaaaccga agatgctgtc ttaatggaga aaatatgtta
    10021 agcgcgcgct acctccacct gcacgaagct ttgggtttag gcccgatgtg gctgaaacag
    10081 gccgccgccg tcctgccgcc caaaaacaca cccgcaaccc cggcacaggc acgtcctcaa
    10141 accgtccgcg ccgccccgat ccgcccttcc caaccccata acggtcaggc gcggctcgaa
    10201 acgatgaaag cgttggaaac cgccgccgta cctacgcgca aacccgcgcc tgaaaccgaa
    10261 acgcctctgc ccggcctttc agacggcatc gcccccgttc ccgccgcttc gggcatcacc
    10321 aagcttgccg tcgtcagcct ttgcccaccg atcgaggatg cggtttacgg gcaactgttc
    10381 cacggcaaag cgggtgtcct gctcgacaac acactcaaag ccgtaggact ggatgccgct
    10441 tatgtacaca aaacctgttg ggtgaaaacc gccgccgtcg gcaacccgat gccgtctgaa
    10501 caggccgtcg cggatgcgct gggtcaaatc gcccgcgaac tcgacagttg ccgcgccccc
    10561 gccgtcctct tcctcggaca ggcttttgtc cagcctgaac ggcaaacgat gattgaaact
    10621 ttgtgcggca gccgtccctt cttcatcatc gaccatcccg cccggctgtt gcgccaaccc
    10681 gaactcaaag cccgcacctg gcaggtgttg aaacagttga aacacgcctt gcaggcaggc
    10741 ggcagttgaa gcaggccgca cggggcggta gaatcacaac tgcatcgcaa tacctgacag
    10801 aaagcaaaaa atgaccgatt tccgccaaga tttccttaaa ttctccctcg cccaaaatgt
    10861 cttaaaattc ggcgaattta ccaccaaggc aggacggcag tcgccttact tcttcaatgc
    10921 tggcctcttt aacgacggct tgtccacgct gcaactggca aaattctatg cacaatccat
    10981 cattgaaagc ggcatccgat tcgatatgct gttcggcccc gcctacaaag gcattatttt
    11041 ggcggcggca actgcgatga tgctggcgga aaaaggcgtg aacgtcccgt tcgcctacaa
    11101 ccgcaaagaa gccaaagacc acggcgaagg cggcgtgttg gtcggcgcgc cgcttaaagg
    11161 gcgcgtgctg attatcgacg acgtgatttc cgccggcaca tccgtacgcg aatcgatcaa
    11221 actgattgaa gcggagggtg caacccccgc cggtgtcgcc atcgcgctcg accgtatgga
    11281 aaaaggcacg ggtgaattga gcgcggttca ggaagtggaa aaacaatacg gcctgcccgt
    11341 cgcccccatc gccagcctga acgatttgtt tattctgttg caaaacaacc ccgaattcgg
    11401 acagttcctc gaacccgtcc gagcctaccg tcggcagtac ggcgtagaat aaaaacaaag
    11461 catatgccgt ccgaaccgcc ttacgcctca gacggcatca aacctgacac acacgaggaa
    11521 ataccatgcc cgcctgtttc tgcccccact gcaaaacccg tctctgggtc aaagaaaccc
    11581 aactcaatgt cgcccaaggc ttcgtcgtct gccaaaaatg cgaaggactg tttaaagcca
    11641 aagaccatct ggcaagcacg aaagaaccca tattcaacga tttgcccgag gctgtttcgg
    11701 atgtcaaact cgttcaccgt atcggcacgc gcgccatcgg caagaaacag atttcccgtg
    11761 acgaaatcgc cggcatcctc aacggcggca caacccagcc cgatattccg cccgcaaccg
    11821 ccgccacccc tgctgccgca ccgcaggtta ccgtaccgcc cgccgcgccc gcccgtcagg
    11881 atgggttcaa ctggacgatt gcaaccctgt ttgcccttat cgtcctcatt atgcagcttt
    11941 cctacctcgt catcctatga tcgtgcccga cctctttgtc gcccacttcc gcgaagccgc
    12001 cccctacatc cgccaaatgc gcggcaaaac gctggtcgcc ggcatagacg accgcctgct
    12061 cgaaggcgat accttaaaca agctcgccgc cgacatcggg ctgttgtcgc aactgggcat
    12121 caggctcgtc ctcatccacg gcgcgcgcca cttcctcgac cgccacgccg ccgcgcaagg
    12181 ccgcacgccg cattattgcc ggggcttgcg cgttaccgac gaaacctcgc tcgaacaggc
    12241 gcagcagttt gccggcaccg tccgcagccg ttttgaagcc gcattgtgcg gcagcgtttc
    12301 cgggttcgcg cgcgcgcctt ccgtcccgct cgtatcgggc aacttcctga ccgcccgtcc
    12361 gataggtgtg attgacggaa ccgatatgga atacgcgggc gttatccgca aaaccgacac
    12421 cgccgccctc cgtttccaac tcgacgcggg caatatcgtc tggctgccgc cgctcggaca
    12481 ttcctacggc ggcaagacct tacatctcga tatgcttcaa accgccgcct ccgccgccgt
    12541 ctcgcttcag gccgaaaaac tcgtttacct gaccctttca gacggcattg cccgccccga
    12601 cggcacgctc gccgaaaccc tctcggcgca ggaagcgcaa tcgctggcgg aacacgccgg
    12661 cggcgaaacg cgacggctga tttcgtccgc cgttgccgcg ctcgaaggcg gcgtgcatcg
    12721 cgtccaaatc ctcaacggag ccgccgacgg cagcctgctg caagaactct tcacccgcaa
    12781 cggcatcggc acgtccattg ccaaagaagc cttcgtctcc atccggcagg cgcacagcgg
    12841 cgacatcccg cacattgccg ccctcatccg cccgctggaa gaacagggca tcctgctgca
    12901 ccgcagccgc gaatacctcg aaaaccacat ttccgaattt tccatcctcg aacacgacgg
    12961 caacctgtac ggttgcgccg ccctgaaaac ctttgccgaa gccgattgcg gcgaaatcgc
    13021 ctgccttgcc gtctcgccgc aggcacagga cggcggctac ggcgaacgcc tgcttgccca
    13081 cattatcgat aaggcgcgcg gcataggcat aagcaggctg ttcgccctct ccacaaatac
    13141 cggcgaatgg tttgccgaac gcggctttca gacggcatcg gaagccgagt tgcccgaagc
    13201 gcggcgcaaa gcctaccgca acaacggacg gaactcgcat attctggtgc gtcgcctgca
    13261 ccgctgaccg caacggaaag ccgccgcaga aatgccgtct gaaccccgtt tcagacggca
    13321 tttccccgat tataaagacc tgcccaaccc tcaaggaccc cgatgaaatc ctaccccgac
    13381 ccctaccgcc attttgaaaa cctcgattcc gccgaaacgc aaaacttcgc tgctgaagcg
    13441 aatgccgaaa cgcgcgcgcg ttttttaaac aacgacaagg cgcgcgcgct ttcagacggc
    13501 attttgaatc aaatgcagga cacgcggcag attccgtttt gtcaggaaca ccgcgcgcgg
    13561 atgtaccatt tccatcagga cgcggaatat ccgaagggcg tgtaccgcgt gtgtacggcg
    13621 gcgacgtacc gttccggcta tcccgagtgg aaaatcctgt tttcagtggc ggatttcgac
    13681 gagctgctcg gcgacgatgt atatctaggc ggcgtgtcgc acttggtgga aaagcccaac
    13741 cgcgcactgt taacactaag caaatcaggc ggcgatacgg cgtacacgct ggaagtggat
    13801 ttggaagcag gggagttggt cgaaggcggt tttcactttc cggcaggcaa aaaccatgtg
    13861 tcgtggcgcg atgaaaacag cgtgtgggtg tgtccggctt gggacgaacg ccagttgacc
    13921 gaatcgggct atccgcgcga ggtgtggctg gtggaacgcg gcaagagttt cgaggaaagc
    13981 ctgcctgtgt atcaaatcga taaaggtgca atgatggtga acgcgtggcg ttatctcgat
    14041 ccgcaaggtt cgccgattga tttgattgaa gcgtcggacg gtttttacac caaaacctat
    14101 ttgcaggtgt cgtcggaagg cgaggcgaaa ccgttaaacc tgcccaacga ttgcgacgtg
    14161 gccggctatc tagccggaca tcttttgctg acattgcgta aagactggca ccgcgcgaac
    14221 caaagctatc cgagcggcgc gctggtagcg gtgaaactga atcgcggcga aatcggggcg
    14281 gcgcagcttt tgtttgcgcc cgatgaaacg caggcattgg aaagcgtgga aacgaccaag
    14341 cgttttgtgg tggcgagcct gctggagaac gtacaaggcc gtctgaaagc gtggcgtttt
    14401 gccgacggca aatggcagga agtcgaactg ccgcgcctgc cttcgggcgc gttggaaatg
    14461 accgaccagc cttggggcgg cgacgtggtt taccttgccg ccggcgattt caccacaccg
    14521 ctgacgctgt ttgcgctgga tttgaacgtg atggaactga ccgtcatgcg ccgccagccg
    14581 cagcagttcg attcagacgg catcgaagtg cggcagtttt ggacgacttc tgccgacggc
    14641 gagcgcattc cttatttcca cgtcggcaaa aacgccgcgc ccgatacgcc gactttggtc
    14701 tatgcctacg gcggtttcgg tattcccgaa ttgccgcatt atctgggcag catcggcaaa
    14761 tactggctgg aagagggcaa cgccttcgtg ttggcgaaca tacgcggcgg cggcgagttc
    14821 ggcccgcgct ggcatcaggc ggcgcagggc atcagcaaac acaaaagcgt cgacgatttg
    14881 cttgccgttg tgcgcgattt gtccgaacgc ggcatgagtt tgcccgaaca catcggtttg
    14941 cagggcggca gcaacggcgg cctgattacc gccgccgcct tcgtgcgcga accgcaaagc
    15001 atcggcgcgc tggtgtgcga agtaccgctg accgacatga tccgctatcc gctgctctcc
    15061 gccggcgcaa gctggacgga cgaatacggc aatccgcaga aatacgaagt ctgcaaacgc
    15121 cagttgggcg aattgtcgcc gtatcacaat ctttcagacg gcatcgatta tccgcccgca
    15181 ctcatcacca ccagcctcag cgacgaccgc gtccatcccg cccacgcgct caagttttac
    15241 gccaaactgc gcgaaacctc gccgcaatct tggctctact cgcctgacgg cggcggccat
    15301 accggcaacg gcacgcaacg cgaatccgcc gacgaactcg cctgcgtgtt gctgtttttg
    15361 aaagagtttt tgggctaagg gcggggagcg gcactgccgc cgcgaatgaa aaaaggtcgt
    15421 ctgaaactgc tttttcagac gacctttttt aatggttgtt tcaaatcaaa atatctatgc
    15481 cgccggcccc atcagcactt cttcacatcc gaaggcaaaa atccgtaatg ccgtctgaac
    15541 gcttcgttga accgtcccgc gtggcggtag ccgcaaaagt gcatggcggc ttggacggtg
    15601 ctgccggatt cgatgagggc gagcgcgtgt tccagccgca ggcggcgcag gcatccggcg
    15661 acggtttcgc cggtttgcgc tttgaaatag cgtttcaggt agcattcgtt cagtccgacg
    15721 cggcgggcga tttcggcgat ggtcagcgga cgggcgaatt cgtgttgcag gatgtcggcg
    15781 gcttcgtcta tgcgccgacg gcggtaaccg ttgtcgtggc ggcggaaggt gaagcgcaat
    15841 aatcgggcgg agagttccag cgcggcggct tcgtcggcaa gcaggccgaa gccgtccgat
    15901 tcgaacgggc gttgcagcag tgggcaggcc gccgccgtca gtgccgctgc gttttgcgcc
    15961 agccgttgca gggcgaatcg gcctatcgtt tgcggcgaaa acaggcgttc gtccagcaag
    16021 ccttcgtcgt gccagcgtcg cagtttttcc agcgaaaaat ccaaatgcag cgcgcacatg
    16081 ccgctgttgt cgggcagcag ggtttcggat acgtccgcca actcgccgcg taccagccag
    16141 atttcgccgg cagatgggcg gtattccctg ccgcccattt gtaaccggtt ctgccccgac
    16201 accatgacga acaaggcgca gttgtggctg aaattgtgga tttcggtggg aaacgcgccc
    16261 gttccgccgc cgcgcatccg cgacaaggtg atgcccgagt cgaagcggtt gatgcacatt
    16321 tccagatgca aaccgggctg ttttgcctgc gcaatgagcg cgctgtcgga acagccgtcc
    16381 aacgcccagc cggatttatc ggagcggaca taggtttggt actggcggta gatggcggcg
    16441 gtgttcatga ttggatagga acgagttgtc taacaaatga attaaatagg aattattacc
    16501 aataatcaag cgcagggatt ggttgaaacg gaaaaggtcg tctgaaaggg tgtttcagac
    16561 gaccttttcc gtatcgggaa tttgttttgc cgtatcggga attttgcgtt ttgcggcgtg
    16621 gtttctgcag gttgtttgct taataataaa cattcttatt cgtatgcaaa ggaaccgcac
    16681 accgtgaaac cgcgttttta ttgggcagcc tgcgccgtcc tgctgaccgc ctgttcgccc
    16741 gaacctgccg ccgaaaaaac tgtatccgcc gcatccgcat ctgccgccac gctgaccgtg
    16801 ccgaccgcgc ggggcgatgc cgttgtgccg aagaatcccg aacgcgtcgc cgtgtacgac
    16861 tgggcggcgt tggatacgct gaccgagctg ggcgtgaatg tgggcgcaac caccgcgccg
    16921 gtgcgcgtgg attatttgca gcctgcattt gacaaggcgg caacggtggg gacgctgttc
    16981 gagcccgatt acgaagccct gcaccgctac aatcctcagc ttgtcattac cggcgggccg
    17041 ggcgcggaag catatgaaca gttggcgaaa aacgcgacca ccatagatct gacggtggac
    17101 aacggcaata tccgcaccag cggcgaaaag cagatggaga ccttggcgcg gattttcggc
    17161 aaggaagcgc gcgcggcgga attgaaggcg cagattgacg cgctgttcgc ccaaacgcgc
    17221 gaagccgcca aaggcaaagg acgcgggctg gtgctgtcgg ttacgggcaa caaggtgtcc
    17281 gccttcggca cgcagtcgcg gttggcaagt tggatacacg gcgacatcgg cctaccgcct
    17341 gtagacgaat ctttacgcaa cgaggggcac gggcagcctg tttccttcga atacatcaaa
    17401 gagaaaaacc ccgattggat tttcatcatc gaccgtaccg ccgccatcgg gcaggaaggg
    17461 ccggcggctg tcgaagtatt ggataacgcg ctggtacgcg gcacgaacgc ttggaagcgc
    17521 aagcaaatca tcgtcatgcc tgccgcgaac tacattgtcg cgggcagcgc gcggcagttg
    17581 attcaggcgg cggagcagtt gaaggcggcg tttgaaaagg cagaacccgt tgcggcgggg
    17641 aaagagtagg gagtcgtctg aaaacggagc ttccgaagga agcggggggt ttctgcgaag
    17701 ctaaagtgcg gtttcaacga attgaaaagc agcctgtatg ttgaaaatac cgctcaagca
    17761 aacctacggt ttgccgccct ctccctagcc ctctcccaca gggagagggg attggggtgc
    17821 aggctgtctt taaggttcag gcaaattttt aacttcgttg aagctccgtt ttcagacgac
    17881 cttttggag
//
LOCUS       DAXIEB010000034        17310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000034 DAXIEB010000000
VERSION     DAXIEB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.034"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(313..1755)
                     /gene="aldA"
                     /locus_tag="ACFOA3_000928"
     CDS             complement(313..1755)
                     /gene="aldA"
                     /locus_tag="ACFOA3_000928"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6897953.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1790..1990)
                     /locus_tag="ACFOA3_000929"
     CDS             complement(1790..1990)
                     /locus_tag="ACFOA3_000929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897954.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1999..2388)
                     /locus_tag="ACFOA3_000930"
     CDS             complement(1999..2388)
                     /locus_tag="ACFOA3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897955.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIEFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2596..3513)
                     /locus_tag="ACFOA3_000931"
     CDS             complement(2596..3513)
                     /locus_tag="ACFOA3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6897956.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKTVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3686..4486
                     /locus_tag="ACFOA3_000932"
     CDS             3686..4486
                     /locus_tag="ACFOA3_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6897957.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLHQDLLG"
     gene            4534..5310
                     /gene="mlaE"
                     /locus_tag="ACFOA3_000933"
     CDS             4534..5310
                     /gene="mlaE"
                     /locus_tag="ACFOA3_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6897958.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5361..5855
                     /gene="mlaD"
                     /locus_tag="ACFOA3_000934"
     CDS             5361..5855
                     /gene="mlaD"
                     /locus_tag="ACFOA3_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6897959.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5892..6482
                     /locus_tag="ACFOA3_000935"
     CDS             5892..6482
                     /locus_tag="ACFOA3_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6897960.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6539..6817
                     /locus_tag="ACFOA3_000936"
     CDS             6539..6817
                     /locus_tag="ACFOA3_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6897961.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6814..7653
                     /locus_tag="ACFOA3_000937"
     CDS             6814..7653
                     /locus_tag="ACFOA3_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6897962.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPG
                     TQP"
     gene            7778..8140
                     /locus_tag="ACFOA3_000938"
     CDS             7778..8140
                     /locus_tag="ACFOA3_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6897963.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8140..8523
                     /locus_tag="ACFOA3_000939"
     CDS             8140..8523
                     /locus_tag="ACFOA3_000939"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6897964.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8520..9008
                     /locus_tag="ACFOA3_000940"
     CDS             8520..9008
                     /locus_tag="ACFOA3_000940"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6897965.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(9090..9218)
                     /locus_tag="ACFOA3_000941"
     CDS             complement(9090..9218)
                     /locus_tag="ACFOA3_000941"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6897966.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9224..9604)
                     /locus_tag="ACFOA3_000942"
     CDS             complement(9224..9604)
                     /locus_tag="ACFOA3_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6897967.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9842..10057
                     /gene="rpmE"
                     /locus_tag="ACFOA3_000943"
     CDS             9842..10057
                     /gene="rpmE"
                     /locus_tag="ACFOA3_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6897968.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            10373..11002
                     /locus_tag="ACFOA3_000944"
     CDS             10373..11002
                     /locus_tag="ACFOA3_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6897969.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDILWTVSGQEKIL"
     gene            11158..12834
                     /locus_tag="ACFOA3_000945"
     CDS             11158..12834
                     /locus_tag="ACFOA3_000945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6897970.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLMLMAFAWLWPGVFSRDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPTLFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            12936..13541
                     /locus_tag="ACFOA3_000946"
     CDS             12936..13541
                     /locus_tag="ACFOA3_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6897971.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LGHYDVKLGKSAAPLLKYAERIFQREAFIDALTPAEKAMRK"
     gene            13610..14002
                     /locus_tag="ACFOA3_000947"
     CDS             13610..14002
                     /locus_tag="ACFOA3_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6897972.1"
                     /translation="MTTSTKPYILRALCEWCSDNAFTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGKAHDIWIPVGHVLSLFARETGEGMGFELE
                     KYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(14031..14180)
                     /locus_tag="ACFOA3_000948"
     CDS             complement(14031..14180)
                     /locus_tag="ACFOA3_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897973.1"
                     /translation="MPSEPHIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(14201..14647)
                     /locus_tag="ACFOA3_000949"
     CDS             complement(14201..14647)
                     /locus_tag="ACFOA3_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6897974.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTDAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VDAAVTETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(14779..14991)
                     /gene="rpsU"
                     /locus_tag="ACFOA3_000950"
     CDS             complement(14779..14991)
                     /gene="rpsU"
                     /locus_tag="ACFOA3_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6897975.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(15280..17130)
                     /gene="ltgA"
                     /locus_tag="ACFOA3_000951"
     CDS             complement(15280..17130)
                     /gene="ltgA"
                     /locus_tag="ACFOA3_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6897976.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPADKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
BASE COUNT         3806 a         4707 c         4482 g         4315 t
ORIGIN      
        1 taaatcaccc gtgtatcgct gtaaatctta gagatggcgg aatatagcgg attaacaaaa
       61 accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca
      121 ccgagtgaat cggttccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt
      181 tttgttaatc cactatacaa agacaaaaac atcgatgtcg gtcatgactg attgccggtg
      241 aaactgtaaa aatgccgtct gaacctgaaa acgggttcag acggcatttt ctatcggggt
      301 tttaaagcgg cattaaatgt cggtttccag ataaacgact tgggtttgca gatattcttc
      361 caaaccgtgt ttgccgtccg cgccgccgat gccggatttt ttccagcccg cgtggaaacc
      421 ctgcatggct tcgaagtttt cgcggttgat gtaggtttcg ccgaattgca ggcggcgggt
      481 aacgtagaag gcttcgttta aattagtcgt ataaacagaa ctggtcagac caaactcgca
      541 atcgttagcc agtgcgatga cttggtcgag cgtgtcgaaa gcggaaacgg gcagcacggg
      601 gccgaaggtt tcttctttca taatgtccat actgttgtcg gtgtcggtca gcagggtcgg
      661 ctcgaagaaa tagccgcgcc cttcggcgcg tttgccgccg cagaccagtt tcgcaccttg
      721 tttgactgcc cgttccactt tttcggcaac ggctttgacg gcgcgctctt caatcagcgg
      781 acccatttcc agcgcgcctg cttcggcttc ggcagggttg ccgtagcgca cgcctttcat
      841 ggcggcggtc attttttcaa tgaacgcgtc tttcaggctg ctgtggacat agacgcgctc
      901 ggcgcagttg cagatttgac cggtgttgcc gacgcgcgaa gccaagatgg atttcaccgc
      961 caaatccaaa tccgcgtctt tcaaaacgat ggcgggggct ttgccgccga gttccagcga
     1021 aactttggtg atgttggcgg aagcggcttc catcacttgg cggcctgctt cgacggagcc
     1081 ggtcaggctg accatatcga cttgcggatg ggcagacaag gcattgccga tttccgcacc
     1141 cggaccgttc accacgttga acacgcctgc gggcaatccg accgcatcga cgatttcggc
     1201 gaagatgtgg cagttgatcg gggttacgct gctgggtttg acaacgatgg tgttgcccgt
     1261 tactaaggcg ggacccattt tgcgggcaat caggaagaaa gggaagttcc acggcaaaat
     1321 gccggcaatt acgcccagcg gacgtttgaa caataaaatg ttttcgcgcg ggcggtcgct
     1381 ttggatgatt tcgccttcgt aacggcgcgc ccattcggct tggtaatcga gatagtcggc
     1441 ggtgaacatc acttccacgc gcgccaagtc tttggttttg ccgccttcgg caacgatggt
     1501 gtcggtcagc tcgtcggcgc gttcgcgtat gccttgggcg attttgcgca aatacgcgcc
     1561 gcgttcgacc gcaggcagac gctcccaagc cggttgcgcc gcacgcgccg ccgctacggc
     1621 gcggtcaacg tccgccctgc cgcctttggg ttcgcgggcg atggcctctt cggtggacgg
     1681 gttcaatacg tcgcgccatt cgccgttgaa atcgttttca aagcgtccgt tgatgtacat
     1741 ggccaattgt ttcatttcgg gttctccagt tttgtagtca gatgtagttt tagtttattc
     1801 ccaaataaat taccgtacaa gtttcttttg aaatgtttag acgagaagct actatgtctg
     1861 catattgttc tgctaattta tattcactcg aatgcaactg ccgattattc caatctgcat
     1921 tcgcaccagt aaaagcccca gcattgccgc ctgtggcata accaatcgct aaactgccaa
     1981 cggtatccac aacaggtttc aggctgcctg aatttctcta ataaatgaaa cacttattct
     2041 atcaaccgtt aatgtaacca aacaattatc aagataagag tagtttatta aatactcatc
     2101 ttcttcaaat tgtatatttt gtagggttag atgattattt tttaattctg ccgttatttc
     2161 atacttgaaa ctatcatcta tttttctaaa ttcaatattt gaagttgatt tatccagtat
     2221 gtaatcatta aagatttcat aggataattc cacttcataa aattgatttt caaatatttt
     2281 gaatgggcag tacgatataa aacaatgtag ttcataacca tttatttcaa tcaatactgc
     2341 ctcttcaatg gtgttatcta actttttgat tttacattta tataacattt atcttgcctt
     2401 tggataaatt gttttgtttt ttggattgtt attcaaagtt ctccaaaagt cttttcctga
     2461 ttttgtatgt atcgtggtaa tactaccatt ttcattggtt ccaacaaaaa taaatttaga
     2521 ttgacctttg gaatcagtac ccaaaaactg aacatatcca gacatgcgga tttcgggttt
     2581 taaatcaaat gcggcttacc ggttttcttt tttggtttcc gcaggcgtgc tgccgaaata
     2641 ttgcctgaat gcctgagtaa agctggaaac gtggcggtag ccgcacaaat acgcggtttc
     2701 gcctatgctt ttgcccccgt tttgcaacag gtagagcgcg tgctgcattt gtttgtggtg
     2761 cagccactcg cttgcggtaa taccgaaatg gtcgcgcata cggcgttgca gcgtcctttc
     2821 actgatgttc agcgcgtctg tcagccggtt gacttggtgt gcgccgtcgg caaacgcggc
     2881 atttagggtg cggttgaagt cttcagacgg catagcgtct gcttccgccg tttgccccgc
     2941 cgccggcccg atgccgtctg aaaccgtgtc ccacaagtcc gacagcagcc gcaaaacgtc
     3001 cgcctcgcgg cgcaatgttt cgcccaaatg cccctttggg acagttttca ggcaggatgc
     3061 cgccaagccg cgcaggttcg ggggcaaatc ccatatcctg accggttcgc ggtaaagcag
     3121 gggtgcgaaa cgcgcgtatt ccggacgcag cagccattgt tccatacctt taattgtcat
     3181 tttgaccgtt ttgccgcctc ggtaaagata gcggctgaac aggacttctt ccccgacagc
     3241 aattaggaca atcttgccgc cgtccgcatc gatttggaag cggcagcggt tgatgccgaa
     3301 gtccaaactg ccttccagca agagcacgaa cgacacataa ggttcggcga ggcggctgct
     3361 gcaaaaatca caccgtgccg ttactgtgcc gccgtggatg gaaatgccgt tggacaaagt
     3421 atcgaaacgg tagctgccct ccacatagct gccgccgccg cttttgacta gattgacgaa
     3481 ccggctgctg ttcaaatgtt ctgctttttc catagttttc tgataaaggt tatcatttga
     3541 aagataacat ttttcgccac agcaaacaat ccgccgccga caacggcatt ttcacaacat
     3601 ttaccgtccg tatgccgtct gaaaattgcc cgtcttcccg ataagcctta aaatacacgc
     3661 cgtcaaacct accgtccgtc cgtacatgag cccatccccc tttatcgaaa tgaaagacgt
     3721 cgccttcgcg tatggcgacc gcccgattct gaagaacatc aatttcagca ttccgcaagg
     3781 caattttgct gccgtaatgg gcggttcggg cagcggcaaa accacgctga tgcgactgat
     3841 tacaggacag attcgtccgc agtccgggca ggttttgatt gaaggacggg atttggcggg
     3901 cttttcggct gacgagttgt atgaacaccg ccgccgtatg ggcgtattgt tccaacacgg
     3961 cgcgctgttt actgatttgt cggtattcga caatatcgct tttccgatgc gcgaactgac
     4021 gcgcctgccg gaagcggtga ttcgggattt ggttttgttg aaattgaacg cggtcggtct
     4081 gcgcggtgtg gaaaacctga tgccgtccga gttgtccggc gggatgtcgc gccgcgtcgc
     4141 gcttgcccgc acgattgcgc tcgaccctga aattatgttg tacgacgagc cgtttaccgg
     4201 cctcgatccg atttccttgg gcgtgattgc ccacttgatc agccgcgtca acaaggcttt
     4261 gcgttcgacc agtattatgg taacgcacga cattgaaaaa tctttggaaa tcgtcgatca
     4321 ggtgattttc ttggcgcacg gcgaaattat gttctccggc tcgccgcagg aaatgcgcga
     4381 actggattcg ccttgggtgc gccagtttgt cggcgggctg gcagacggtc ccgtagcata
     4441 ccgctatccg gcgcaaacgt cgttgcatca ggatttgctc gggtaaatga gccggtgccg
     4501 tctgaaaccg ttgaacttca catatagaaa tttatgaatt ttatccgttc cgtcggggcg
     4561 aaaaccctcg gccttattca atccctcggc agtattacgc tgtttctgct gaacattctg
     4621 gcgaagtcag gtacggcttt cgtccgtccg cgcctgagcg tgcgccaagt gtatttcgcc
     4681 ggcgtgctgt cggtgctgat tgttgccgtt tcagggctgt tcgtcggcat ggtcttgggt
     4741 ttgcagggct atacgcagtt gtcgaaattc aaatccgccg atattttggg ctatatggtt
     4801 gcggcttcgc tgttgcgcga actgggtccg gtgttggcgg cgattctgtt tgccagcagc
     4861 gcgggcggtg cgatgaccag cgaaatcggt ttgatgaaaa cgaccgaaca gctcgaagcg
     4921 atgaacgtga tggcggtaaa ccccgtcgcc cgagtggttg cgccgcgctt ttgggcgggc
     4981 gtgttttcca tgccgctttt ggcttcgatt ttcaacgtgg cgggtatttt cggcgcgtat
     5041 ttggtcggtg taacctggct gggcttggac agcggtattt tctggtcgca aatgcagaac
     5101 aatatcacga tacattacga tgtaatcaac ggtctgatta aatccgccgc gttcggcgtg
     5161 gcggtaacgc tgattgccgt gcatcagggc ttccattgcg tcccgacttc ggaaggcatt
     5221 ttgcgcgcca gcacgcgcac ggtggtttcg tccgccctga cgattttggc ggtcgatttt
     5281 atattgaccg cgtggatgtt tacagattga caggccgtct gaagacgaaa cgcggaacat
     5341 cggatattca aggaacttta atgaaaaaga acatattgga attttgggtc ggactgttcg
     5401 tcctgattgg cgcggcggcg gttgcctttc tcgctttccg cgtggccggc ggtgcggcgt
     5461 tcggcggttc ggacaaaact tacgccgttt atgccgattt cggcgacatc ggcggtttga
     5521 aggtcaatgc ccccgtcaaa tccgcaggcg tattggtcgg gcgcgtcggc gctatcggac
     5581 ttgacccgaa atcctatcag gcgagggtgc gcctcgattt ggacggcaag tatcagttca
     5641 gcagcgacgt ttccgcgcaa atcctgactt cgggactttt gggcgagcag tacatcgggc
     5701 tgcagcaggg cggcgacacg gaaaaccttg ctgccggcga caccatctcc gtaaccagtt
     5761 ctgcaatggt tctggaaaac cttatcggca aattcatgac gagttttgcc gagaaaaatg
     5821 ccgacggcgg caatgcggaa aaagccgccg aataaacgta tgccatcata ttcaaactag
     5881 gacacttaat catgaaaaaa tcctccctca tcagcgcatt gggcatcggt attttgagca
     5941 tcggcatggc atttgccgcc cctgccgacg cggtaagcca aatccgtcaa aacgccactc
     6001 aagtattgag catcttaaaa aacggcgatg ccaacaccgc ccgccaaaaa gccgaagcct
     6061 atgcgattcc ctatttcgat ttccaacgta tgaccgcatt ggcggtcggc aacccttggc
     6121 gcaccgcgtc cgacgcgcaa aaacaagcgt tggccaaaga atttcaaacc ctgctgatcc
     6181 gcacctattc cggcacgatg ctgaaattaa aaaacgccaa cgtcaacgtc aaagacaatc
     6241 ccatcgtcaa taaaggcggc aaagaaatca tcgtccgcgc cgaagtcggc gtacccgggc
     6301 aaaaacccgt caacatggac ttcaccacct accaaagcgg cggtaaatac cgtacctaca
     6361 acgtcgccat cgaaggcgcg agcctggtta ccgtgtaccg caaccaattc ggcgaaatta
     6421 tcaaagcgaa aggcgtggac ggactgattg ccgagttgaa ggctaaaaac ggcagcaagt
     6481 aatccataag ccatgccgcc agactgaagg cggcatggat tttttcctac cgaacactat
     6541 gcatacagaa ctcaacaacg gaacactgca catcagcggc gacgtaaccg tcaaaaccct
     6601 gaccgccgcc gcctttaccc gcttcagaca gcaatgtcgt ctgaaagaca cccgcgcggt
     6661 tgatttgagc ggcgtcggac gcgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct
     6721 gcgcggttgc aaaggcagcg tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc
     6781 cgaactgtac gaaatcaaag actggctgaa atcatgaaaa aaaccgccta tgccttcctc
     6841 ctgctgatcg ggttcgcttc cgcccctgca tttgccgaaa cccgccccgc cgacccttat
     6901 gaaggctaca accgcgccgt tttcaaattc aacgaccaag ccgaccgcta cattttcgcc
     6961 cctgccgcgc gcggctaccg caaagttgcg ccgaaacccg tccgcgccgg cgtgtccaat
     7021 ttttttaaca acctgtgcga cgtggtcagc ttcggcagca atatcttgcg cttggacatc
     7081 aaacgcgcaa gcgaagacct tgtccgcgtc ggcatcaaca ccactttcgg tttgggcggg
     7141 cttatcgaca tcgccggcgc gggcggcatt cccgacaata aaaacacctt gggcgacacg
     7201 tttgcctcgt ggggatggaa aaacagcaat tatttcgtgt tgcccgtctt agggccgtcc
     7261 accgtccgcg acgcgctcgg cacgggtatt acctccgttt attcgcccaa gaatatcgtc
     7321 ttccgcaccc ctgtcggacg ctggggcacg actgccgtat ccgccgtcag tacgcgcgaa
     7381 ggcctgctcg atttgaccga cagtctggac gaagccgcca tcgacaaata cagctacacg
     7441 cgcgacctct atatgaaagt ccgtgcgcgg cagaccggtg caacacctgc cgaaggtacg
     7501 gaagataaca tcgacatcga cgaattggtc gaaagtgccg aaaccggcgc ggcggaaact
     7561 gccgttcaag aagattccgt atccgaaaca caggcagaag cagcagggga agccgaaacg
     7621 caacctggaa cacaacctgg aacacaacct taaagccatg ccgtctgaaa atccgtgttc
     7681 ggacggcatt cccttctgtc ggaacggaca gtccgaaacc acagattggg ctataatctc
     7741 ttttttgcgc ataccgcgcg acagcaaagg aaaaaccatg tacgaagtca accgcagcgt
     7801 attcgtcctc atccctttag aacctttttg gaactggctg caaaccctgc cgggcaacca
     7861 ccttgacgga ctgaccctcg aagacatcca agccgatgcc aattcctacc ttgtccgccc
     7921 gtgcgaaacc gccgacgaag tatgggacga aatcgaagcc cgttttgaag acattttcgc
     7981 cgccgaactt gccgactggt gcgaagacga acgcgagtgg cctgcgctcg atgccgacat
     8041 tttcaacgaa tggttcgaca tccagctttc taccgtcatc accgacctcg aacacgaacc
     8101 gcttgcccgc gaagccttcc aacccatcaa cctgaactga tgaagctgac cgtccgcaac
     8161 taccatctcg acggctacgg ccatgtcaac aatgcgcgct acctcgaatt tttcgaagaa
     8221 gcgcgctggg cgttttttga agaacgcgga ctgctgcacg agttggcagg cctcatactg
     8281 attgtcgccc gcatcgacat ccgatacagc cgccccgccg tcgaaggcga cgtattgcag
     8341 ttttcatgcc gcctgaaaac ccccggcacg cgccgtattg tgctgaccca gacgattaca
     8401 ctgccaaacg gcaaaaccgc cgcagaagcc gacatcaccc tgatgcccgt ccacgccgcc
     8461 acacaacgca ccgtcagcct gcccgccacc cttgcccgcg cattggaagc cttgtccgaa
     8521 tgaaaaaaat actcaccgcc gccgtcgtcg cactgatcgg catcctcctt gccatcgtcc
     8581 tcatccccga cagcaaaacc gcgcccgctt tctccctgtc cgacctgcac ggaaaaaccg
     8641 tttccaacgc cgacctgcaa ggcaaagtta ccctgattaa tttttggttt ccctcctgtc
     8701 cgggttgtgt gagcgaaatg cccaaaatca ttaaaacggc aaatgactat aaaaacaaaa
     8761 acttccaagt ccttgccgtc gcccagccca tcgatccgat agaaagcgtc cgccaatatg
     8821 tcaaagacta cggtttgccg tttaccgtca tgtatgatgc ggacaaagct gtcggacagg
     8881 cgttcggcac acaggtttat ccgacttccg tccttatcgg caaaaaaggc gaaatcctca
     8941 aaacttatgt cggcgaaccc gatttcggca aactctacca agaaatcgat accgcgctgg
     9001 cacaatagcg atgccgtctg aagcgcaaaa aaacagaaac gatgttcctg ttttttcaga
     9061 tcatccgccc cgacacgttc agacggcatt caaccttccg gcagattctt tttcaccact
     9121 gccggattgc ctgccgccaa agagtagggc ggaatgtctt ttgtaaccac cgcgcctgcg
     9181 cccacgaccg aaccgcgtcc gacggttacg cccgccatta caatcaccct gtgccccggc
     9241 cagacatcgt cctccaacgt aatcggacgg atttccgtgt agccctcaaa gcgttttttg
     9301 aacggtcaaa cttgtggtta tttgaataaa acagacattc cggctccatc atcacatttt
     9361 tgccgaccac cagcccacgg cagatttcac agtttgcccc gatgcccgag ccgtcgccca
     9421 aaaccgtatc cggaaacaca tacgccccgc gttcgatatt gaccccgcgt ccgatatgcg
     9481 gagaaacccg ccgcgccaaa aaaccgcgca cccgtctgcc gacgatgccg atgccgcgca
     9541 tatgtgacgg cggcaacccc gaaccggcaa aacgcagcag caggaaaaag aattttgatc
     9601 ccatcaaacc tccctcaaaa gaacttttta tagtggatta acaaaaatca ggacaaggcg
     9661 acgaagccgc agacagtaca gatagtacgg caaggcgagg caacgctgta ctggtttttg
     9721 ttaatccact atacatttta tcgaatatcc tcttgacgaa acaacagaaa atagcgagaa
     9781 tgccgagctt gtctgaagca taggtacatc ccctatgccc gcttttttga aaggaaatca
     9841 aatgaaacaa ggtattcacc cgaactacca cgaagtcaac gttacctgct cttgcggcaa
     9901 caaattcgca accaaatccg cgatggaaaa agaaaacttc aatatcgagg tttgctccct
     9961 gtgccacccg ttctataccg gcacccaaaa aatcgttgat accaccggcc gcgtggacaa
    10021 attcaacaac aaattcggca acctgttcaa acgctgattg ccgctttgca agaattaagc
    10081 cctgccatca tttcgatggc agggcttttt aatatttggc acactgactt cttatctata
    10141 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
    10201 aaccgattca ctcggtgctt gagcacctta gagaatcgtt ctctttgagc taaggcgagg
    10261 caacgccgta ctggtttttg ttaatccact atattttgat ggggttgaag ttgacatcta
    10321 caaagtttaa tgtttgaata cattaaaaaa atatatttga atgagatgtt ttatgattca
    10381 aaatgtcgtt acttcaataa tcctgtattc tgggacagcc gtagacttac ttattatcct
    10441 aatgttattt tttgccaaaa gaaaaagtag aaaagacatc attaacatct atttaggaca
    10501 atttctaggc tctgttagcc tgatattgct aagtttgctt tttgcatttg tcttagatta
    10561 tattcctagt aaagagattt taggtttgct cggcttgatt ccaattttcc taggcatcaa
    10621 agttttgctg ttaggagatt ctgatggaga ggctattgcc aaagagggtt tgcgcaaaga
    10681 taataaaaac ctgatttttc tagtcgctat gattactttt gcaagttgtg gtgctgacaa
    10741 tattggtgtc tttgtcccat attttactac cttaaattta gcaaatttga tagtggcttt
    10801 acttaccttt ctagtcatga tttatctctt ggttttttct gcccaaaaat tggcacaagt
    10861 cccttctgtt ggagaaactt tggaaaaata tagcagatgg tttgttgccg ttgtttattt
    10921 aggattgggg atatatatcc tgattgaaaa caacagtttt gatatactat ggactgtgtc
    10981 gggccaggaa aaaatattat gaaaaagata gtatctgcaa agactgccat gtgcagtctt
    11041 ttttgttgcc ggtctttttg tgtctgatgc tgtcggattt tatctgatgc cgtctctgca
    11101 cgacctttgt gcgaatattt gctacacttg gcaatccgtc cattttttct cacgcttatg
    11161 ctgacctaca ctccgcccga tgcccgcccg cccgccaaaa cccacgaaaa accgtggctg
    11221 ctgatgctga tggcgtttgc ctggttgtgg cccggcgtgt tttcccgtga tttgtggaat
    11281 cctgccgaac ctgccgtcta taccgccgtc gaagcactgg caggcagccc cacccccttg
    11341 gttgcccatc tgttcggtca aaccgatttc ggcataccgc ccgtgtatct ttgggttgcc
    11401 gccgcgttca aacatttgct gtcgccgtgg gctgccgact catacgatgc cgcacgcttt
    11461 gcaggcgtgt ttttcgccgt tgtcggactg acttcctgcg gctttgccgg tttcaacttt
    11521 ttgggcagac accacgggcg cagcgtcgtc ctgattctca tcggctgtat cgggctgatt
    11581 ccagttgccc atttcctcaa ccccactgcc gccgcctttg ccgccgccgg actggtgctg
    11641 cacggttatt ctttggctcg ccggcgcgtg attgccgcct ctttcctgct cggtacgggt
    11701 tggacgctga tgtcgttggc agcagcttat ccggcagcat ttgcgctgat gctgcccttg
    11761 cccgtgctga tgtttttccg tccgtggcaa agcaggcgtt tgatgttgac ggcagtcgcc
    11821 tcgcttgcct ttgccctgcc gcttatgacc gtttacccac tgctcttggc aaaaacgcag
    11881 cccacgctgt tcgcgcaatg gctcgactat cacgttttcg gtacattcgg cggcgtgcgg
    11941 cacgttcaga cggcattcag tttgttttac tatctgaaaa acctgctttg gtttgcattg
    12001 cccgcgctgc cgctggcggt ttggacggta tgccgcacgc gcctgttttc gaccgactgg
    12061 gggattttgg gcgtcgtctg gatgcttgcc gttttggtgc tgcttgccgt caatccgcag
    12121 cgttttcagg ataacctcgt ctggctgctt ccgccgcttg ccctgttcgg cgcggcgcaa
    12181 ctggacagcc tgaggcgcgg cgcggcggcg tttgtcaact ggttcggcat tatggcgttc
    12241 ggactgtttg ccgtgttcct gtggacgggc tttttcgcca tgaattacgg ctggcccgcc
    12301 aagcttgccg aacgcgccgc ctatttcagc ccgtattacg ttcccgacat cgatcccatt
    12361 ccgatggcgg ttgccgtact gttcacaccc ttgtggctgt gggcgattac ccggaaaaac
    12421 atacgcggca ggcaggcggt taccaactgg gcggcaggcg ttaccctgac ctgggctttg
    12481 ctgatgacgc tgttcctgcc gtggctggac gcggcgaaaa gccatgcgcc ggtcgtccgg
    12541 agtatggagg catcgctttc cccggaattg aaacgggagc tttcagacgg catcgagtgt
    12601 atcgacatag gcggcggcga cctgcacacg cggattgttt ggacgcagta cggcacattg
    12661 ccgcaccgcg tcggcgatgt acaatgccgc taccgtatcg tccgcttgcc ccaaaatgcg
    12721 gatgcgtcgc aaggctggca gacggtctgg cagggtgcgc gcccgcgcaa caaagacagt
    12781 aagttcgcac tgatacggaa aatcggggaa aatatattaa aaacaacaga ttgagccgaa
    12841 tttctggatt aagtgccgga aatcgcgtat aattgcgcga ttaaaccttt ataaatcagc
    12901 cgttttgcag gcatcacaca ggagcgacaa ttattatgat gaccctctat tccggcatta
    12961 cctgcccctt cagccaccgc tgccgcttcg ttttgtacga aaaaggtatg gattttgaaa
    13021 tcaaagacgt cgatatttac aacaaacccg aagacctcgc cgtcatgaat ccgtataacc
    13081 aagttcccgt gctggtcgag cgcgatttgg tgctgcacga gtccaacatc atcaacgaat
    13141 acatcgacga acgcttccct catccgcagc ttatgcccgg cgatcccgtt atgcgcggcc
    13201 ggggccgact ggtgctgtac cgtatggaaa aagaattgtt caaccacgtc caagtgttag
    13261 aaaaccccgc cgccaccaac aaggaacagg caaaagcgcg cgaagccatc ggcaacggtc
    13321 tgaccatgct tgccccttcg ttcagcaaaa gcaaatacat cctcggcgaa gatttttcta
    13381 tgattgatgt cgcccttgct ccgctgctgt ggcggctcgg ccactacgat gtcaaactgg
    13441 gcaaaagtgc cgcgccgctg ctcaaatacg ccgagcgcat cttccagcgc gaagccttta
    13501 tcgacgcgct gacacctgcg gaaaaggcga tgcgcaaata agtttgaaat gcctgtaaaa
    13561 cctgacggtt tcaggcattt ctatattcag gcacattatg gggagcatta tgactacttc
    13621 caccaaaccc tacattctcc gcgccctctg cgaatggtgc agcgacaacg cctttacccc
    13681 tcacatcctt gtctgggtca acgaacacac gcgcgtccct atgcagtacg tccgcgacaa
    13741 cgaaattatg ctcaacatcg gcgcgaccgc cacgcaaaac cttcaaatcg acaatgattg
    13801 gatcagtttt tccgcccgtt tcggcggaaa ggcacacgac atatggatac ctgtcggaca
    13861 cgtcctcagc cttttcgcac gggagaccgg cgaaggtatg gggtttgaat tggagaagta
    13921 ccgacccgat acgccgcctg aaaacacctc tgccgaaacc gcgccccggc ccgccaaaaa
    13981 aggcttgaaa ttggtcaaat aaatctatgc cgtctgaacg gaatcgtgtt tcagacggca
    14041 ttttgtccga tggggcgcaa acggaatccg tttatcggca aaacccgttt cggtgtatcg
    14101 aaaccgtgtt gcccctgccg attcgatatt gggacattga aaatgccgtc tgaacctgcg
    14161 atatggggtt cagacggcat ttttccttat attcatacaa tcaggcggtc agtacggctt
    14221 tcagaatttt gttgacttcg cccatatcgg ctttgccggc gaggcgggtt ttcaatacgc
    14281 ccatcacttt gcccatatcc gccatacctg ccgcgccggt ttcggtaacg gcggcatcga
    14341 cctcggtacg gatttcgccg gcggaaagca tttggggaag gtagcggtgc agtacctcga
    14401 tttcggcgtt ttctttgtct gccaagtcct gccgccccgc gtcagtatag attttcgcgc
    14461 tgtctttgcg ctgtttgacc attttggtca ggatggcggt gattttggca tcgtcggctt
    14521 cggtgcgttc gtccacttca aactgtttga cggcggcgtt gataaggcgg atggtgccga
    14581 gggaaacttg gtctttggcg cgcatagccg ttttcatgtc ttcggtaagg cggattttca
    14641 ggctcataat gttctcgctg ggatgtcgga tggaaacggc gggtttcgat gccgtctgaa
    14701 aagcaaaaca caccgcagag caggtcttgc ggtgtgtcct catatcacag ggctgacctg
    14761 taatctatac ttgaatgttt agtacatttt gggcggcagt tgttggctgc gcaggcgttt
    14821 ttgcaggcgt tttacggctg ccgctttttt gcgtttgcgt tcggtagtcg gtttttcgta
    14881 ggcttcgcgg gcgcgcagct cggtcagcag gccggttttt tctacggcgc gtttgaaacg
    14941 gcgcatagcg acttcaaatg gttcattctc ttttacgcgg attgcaggca ttttatttcc
    15001 tttaataaat tcggttgttt catctgccca tcatatcggt ggaaagggta gggcagacgg
    15061 tgttgtaggt tttccgtatc gctgtgcctt tcggcggcgg gatacgcgcc gtgacggaaa
    15121 catcacctcc taacgggtcg gcttgtgccg tgtcaagctg cttgaaaaag caggaaaaac
    15181 aattttcgga ttgtcttata tttatggttc gccgtcaatg ccgttcggga taaaaatgcc
    15241 gtctgaaagg ccgggcgagc ttcagacggc atcggtacgt cagcgggcgg gaacaatgcc
    15301 catacgctgt ttgagcggga tgtgcggcgc gccgaagagg gcggcgtagt aggcggcatt
    15361 ggtcatcact tttttgacat agtcgcgcgt ttcggaaaac gggatggttt cggcatatac
    15421 cgcgccttcg aggggcgtgt ccgcctgcca tcgacgcgcc ctgccggggc cggcgttgta
    15481 gcctgcggtg gcgaggactt cgttgttttg caggcggcgt ttggtgtccg ccatatacca
    15541 cgtccccata cggatattgc cgtcggcggt gtaaagttgt gcggcatcca taccgatttt
    15601 gccggcgatt tcgcgcgcgg tggcaggcat aacctgcatc agcccctgcg cgcctacgcg
    15661 ggattgcgcg cccataacga agcggctttc ctgacgaatc agcccgtaaa cccacgccgg
    15721 atcgacatta acattttgcg cgtggcggat taccgtgtct ttaaacggcg aaatgtagcg
    15781 caaggtgtag ttgagtttgc ggtcggtgcg ttccgcgctg ttgaccgcca tatcgtaaaa
    15841 accgtggtcg aacgcggttt gcgcggcggt cagcagcttg tcttcatcga agccgcgtgt
    15901 ggcgaaacgc cattccgcct gagcctgacg gcgcattttc gcatcgccgg cggttcggct
    15961 gtttcggaac agcaccagcg cgcgcttaat cgcgccgtct tccgccatac ggaggacgct
    16021 gtttttgccg gcatcgggca cattgttgcg cgtatcgatt ttgcgcccca actcttcccc
    16081 cgccagcacc gcataaaaat tcctgcccgt cgctgccgcc tgtttgtaaa gtttttccgc
    16141 ctcttgcgtg ttgcccgttg cggcgcgact gcgtgccagc cagtagagcc aggtcgggct
    16201 tttttgcagt ttttcgggca tatgcgagat tacggaagcc agctcgtccc aacgtcgggc
    16261 gcgcaaggcg gcgcgggcgt accactcgat ttggtcgtcg gtcagttggc ggcggtcggc
    16321 aactttgccg taatagtcca aggcggcagg cacattgagg ctttgcgact gataatgccc
    16381 caatacgccc cacgcgaaac tgcgttgttc gaggcttaaa ccgctttcca tttcggacag
    16441 cagggcggcg gcattcggcg atttgcgtgc ttctttgccg atgacgttca acagggcata
    16501 ttcgcgcgaa ccttgtgtac cgccgtcaaa cgggctgccc aatgcggcgg caaggttgcg
    16561 tgcgtctgtg gtttggcggc cggccagcag tccgcgcacg cgcctccagg cgtcgttgcc
    16621 gtccaacaag ccggatgcgg ctgcctgttc caacagtttg gtgcagcccg aaggcagttt
    16681 gcccgtattt ttgaccagtt cggcggcacg cgtatagtcg ttgcggctcg aatcggcgta
    16741 gcattcgact tcttgggcgc gccctgccgg ttcgagtttg gcgtattcct gtgcaaacag
    16801 cgtccactgt ttgcgtgcgc ccaaagactt cagccactcg ttgcggacat tttccgccat
    16861 cgcactgtcg ccggcgtttt ccaaataggc ggcgacggcg gcatcgtttt tctgtttcac
    16921 tgcatccagt gcggacgggt agccgccgta atctgccaac gtttttcctt cgggttcggc
    16981 agggcgggtg ggaacgcttg ccgaaaggtc ggcagtttct atattgtctg ccggggtctt
    17041 gtcggctggc agtgtgtttg tcgaagaaca cgcggcaagc accagggccg ccagcagcgg
    17101 cagggaatgc ttcatagagg gtaggtacat cggatttcct taagaatcgg aaccctgaac
    17161 ggtcagggtt ggaaaagaca aaatgccgtc tgaacaggcg tttgcatgaa ttatatgccg
    17221 aaactgcacc ggctttggaa tgtttccgac ataatttata tttttcaatc atttgccgtt
    17281 ttggtgcgaa ccgctgcctt tgcccgtttc
//
LOCUS       DAXIEB010000035        17162 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000035 DAXIEB010000000
VERSION     DAXIEB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.035"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(76..1245)
                     /gene="metK"
                     /locus_tag="ACFOA3_000952"
     CDS             complement(76..1245)
                     /gene="metK"
                     /locus_tag="ACFOA3_000952"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6897977.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1428..1538)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1591..2487
                     /locus_tag="ACFOA3_000953"
     CDS             1591..2487
                     /locus_tag="ACFOA3_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6897978.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQTGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKTHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKVP"
     gene            complement(2531..3595)
                     /gene="tsaD"
                     /locus_tag="ACFOA3_000954"
     CDS             complement(2531..3595)
                     /gene="tsaD"
                     /locus_tag="ACFOA3_000954"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6897979.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGVSYGDIDAVAFTQGPGLGGALLAGSSYANALAFAL
                     DKPVISVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFSPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3711..4898)
                     /gene="ccsB"
                     /locus_tag="ACFOA3_000955"
     CDS             complement(3711..4898)
                     /gene="ccsB"
                     /locus_tag="ACFOA3_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6897980.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LFSLRVEGKGGKSWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4891..6843)
                     /locus_tag="ACFOA3_000956"
     CDS             complement(4891..6843)
                     /locus_tag="ACFOA3_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6897981.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLSADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSMRQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7120..7743)
                     /locus_tag="ACFOA3_000957"
     CDS             complement(7120..7743)
                     /locus_tag="ACFOA3_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6897982.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7949..8578
                     /gene="yihA"
                     /locus_tag="ACFOA3_000958"
     CDS             7949..8578
                     /gene="yihA"
                     /locus_tag="ACFOA3_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6897983.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8699..11095)
                     /locus_tag="ACFOA3_000959"
     CDS             complement(8699..11095)
                     /locus_tag="ACFOA3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6897984.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11247..12362
                     /gene="pilM"
                     /locus_tag="ACFOA3_000960"
     CDS             11247..12362
                     /gene="pilM"
                     /locus_tag="ACFOA3_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6897985.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQSNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12365..12964
                     /locus_tag="ACFOA3_000961"
     CDS             12365..12964
                     /locus_tag="ACFOA3_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6897986.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12965..13612
                     /locus_tag="ACFOA3_000962"
     CDS             12965..13612
                     /locus_tag="ACFOA3_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6897987.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13630..14175
                     /locus_tag="ACFOA3_000963"
     CDS             13630..14175
                     /locus_tag="ACFOA3_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6897988.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14194..16479
                     /gene="pilQ"
                     /locus_tag="ACFOA3_000964"
     CDS             14194..16479
                     /gene="pilQ"
                     /locus_tag="ACFOA3_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6897989.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4623 a         4883 c         3899 g         3757 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actatataaa aatgccgtct gaaccttcag acggcatttc
       61 tttattcaga ccaaattaca gaccggcggc tgccctcaat gcggctgctt tgtcggtgcg
      121 ctcccaagta aactcgggtt cttcgcggcc gaaatgtccg taagcggcgg atttactgta
      181 aatcgggcgc aagaggtcga gcatttggac gatgcctttg gggcgcaggt cgaaatgttc
      241 gcgaactaag gtaatcagtt tttcttcgct gattttgcct gtgccgaaag tatcgatgga
      301 aatcgaagtc ggttcggcaa cgccgatggc gtaggaaact tgaatttggc attgggttgc
      361 caagcctgcg gcgacaatgt ttttcgccac ataacggcag gcataggcgg cggaacggtc
      421 gactttggac gggtctttgc cggagaacgc gccgccgccg tgcggagccg cgccgccgta
      481 ggtatcgacg atgattttac ggccggtcaa accgcagtcg ccttgcggac cgccgatgac
      541 gaagcggccg gtcggattga tcaggtattt ggtttcgtcg gtcagtagtt cagacggcag
      601 aacgggcttg ataatctgct caatgacggc gtttttcagc tcttcataac cgatttccgg
      661 gtcatgttgc gtagagagga cgacggtatc aatgcgtttt accttaccgg tttcgctgtc
      721 gtaaaccacg gtcaactgcg cttttgcgtc gggacgcagc cacggcaggc ggccgtcttt
      781 gcgcaattcg ctttggcgtt gcatcagacg gtggctgtaa tagatggcaa acggcatcag
      841 ggtcggggtt tcgtcgcagg cgtagccgaa catcagacct tggtcgcctg cgccttggtt
      901 caagtcgatg ccttcgcctt cgttcacgcc ttgggcgatg tccggagatt gctggtcgta
      961 gtacacgccg accgcgcagc cgttggcatc aaagcccagc tcggaggagt tgtagccgat
     1021 gcgtttgatg gtttcgcgtg cgactttgat gtagtccact tgggcggtgg tggtgatttc
     1081 gcctgccaat acgcacaagc ctgtgttgac caaagtttcc gccgcgacac gcgctttggg
     1141 gtcttgcgcc agaatcgcat ccaaaatagc atcggatact tggtcggcca ctttgtccgg
     1201 atggccttcg gataccgatt cggaagtaaa cagatattcg ctcattgaga ttttccttga
     1261 aaaacaaacc atcttcttca gacggcatgt tgtatgaaca taatgtcgac agcgggaaat
     1321 ataacaaaat ttccctattc ataccattca gttgagaaat attcccattt gaatggcact
     1381 ttggaatctc tgcccgtacg tttcgtacag gcaaaaaaat tcccgcatca agcgggtttg
     1441 gattgctctg gtgagccaca tcggcttttc aaccgtccac cttacttttc cttttgaaaa
     1501 gcaggttggc atggaattcc caactcttaa tgcagcgcgg atggtagcag aaaagccttt
     1561 ccgccgcaat acttcccttt ttcagacggc atgtttcgtt tacaattcgg gctgtttccc
     1621 cctttgcgaa ccgccatgca catcctgttg accgccctgc tcaaatgcct ctccctgctg
     1681 ccactttcct gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta
     1741 aaggaagacc gcgcgcgcat cgtcgccaat atgcggcagg caggcatgaa tcccgacccc
     1801 aaaacggtca aagccgtttt tgcggaaacg gcaaaaggcg gtttggaact tgcccccgcg
     1861 tttttcaaaa aaccggaaga catcgaaaca atgttcaaag cggtacacgg ctgggaacat
     1921 gtgcagcagg ctttggacaa gggcgaaggg ctgctgttca tcacgccgca catcggcagc
     1981 tacgatttgg gcggacgcta catcagccag cagcttccgt tcccgctgac cgccatgtac
     2041 aaaccgccga aaatcaaagc gatagacaaa atcatgcaga cgggcagggt tcgcggcaaa
     2101 ggcaaaaccg cgcctaccag catacaaggg gtcaaacaaa tcatcaaagc cctgcgttcg
     2161 ggcgaagcaa ccatcgtcct gcccgaccac gtcccctccc ctcaagaagg tggggaaggc
     2221 gtatgggtgg atttcttcgg caaacctgcc tataccatga cgctggcggc aaaattggca
     2281 cacgtcaaag gcgtgaaaac cctgtttttc tgctgcgaac gcctgcctgg cggacagggt
     2341 ttcgatttgc acatccgccc cgtccaaggg gaattgaacg gcgacaaaac ccatgatgcc
     2401 gccgtattca accgcaatac cgaatattgg atacgccgct ttcctaccca gtatctgttt
     2461 atgtacaacc gctataaggt accgtaacga aaataaaaat gccgtctgaa caatttcaga
     2521 cggcattttg tcatctgacg atttccgaca gcggccagcg cggacggaca ttgaacgcac
     2581 cgacctccct gcccttgccc aggtgcatcg caccggcaaa ggcaatcatg gcaccgttgt
     2641 ccgtgcagta tgccatcggc gagaaaaaca cgctgacttt ttcggacgga tgtttcggct
     2701 tgcctttggg ggtcgggatt tgcaccgtca tgttgccgaa agtttcgcgg agtttgcggt
     2761 ttgcaccgac cccgccggcg accactacgg ttctgaaccc tgtctgcaac agggcttttt
     2821 tcacttttgc cgccaacaca tctaccaccg catcttgaaa cgcacggcag atgtcgttgc
     2881 gtgtctgctc aggaatgtca tccgccccgt tttccgcgcg cactttctca acggcggtca
     2941 atacggcggt tttcaaacct gaaaaactca tctgcaaatc gtcggaatga atcatcgggc
     3001 gcggaaaaac aaacgcttcg ggcctgcccg attccgcaag ttccgacagt ttcgcaccgc
     3061 ccggatacgg caagcccagc agtttcgccg ttttgtcgaa tgcctcgccc gccgcatcat
     3121 cgacgctctc gcccaaaagc tcgtagtcgc ctatgcccct gaccgccata atctgcgtat
     3181 gcccgcccga aaccaacagc gcgacaaaag gaaagtcggg tttttcctcc gccaacaggg
     3241 gggaaagtaa atgtccttct aaatgatgga cagagataac gggcttatcc agtgcaaaag
     3301 ccagagcatt ggcgtagctc gaacccgcca gcagcgcacc gcccaaaccg ggtccctgcg
     3361 taaaggcaac cgcgtcaatg tcgccatacg atacgcctgc ctgcgccaaa cagccttccg
     3421 tcaacggaac aaggcggcgg atatggtcgc ggcttgccaa ttccggcaca accccgccgt
     3481 attcggcgtg cattgccatc tgcgtgtgca ggcagtgcgc ccgcaatcct cgttcggtgt
     3541 catacagcgc aacgccggtt tcgtcgcaag aagattcgat tcccaatacc aacatggtct
     3601 gatgccgtta aaaactgaaa aacgtatttt agcggatttc ggcacgactg ccgtatccca
     3661 aaaacggaac atgccgtctg aagcccttca gacggcatcg tcgcaccgta tcaaagcgtt
     3721 ccgtaagaat gcagcccgct caaaaacata ttcacgccga taaaggcaaa tgcggttacg
     3781 aacaaaccga taatcgccca ccacgccagc actttgccgc gccaaccggc aaccagccgc
     3841 aaatgcagcc aaacggcgta attgagccag acgatgaacg cccacgtctc tttcggatcc
     3901 caactccaat agcgtcccca agcatccgcc gcccacagcg cacccaaaat ggtggcaatg
     3961 gtaaagaaca gaaagccgac ggcaatcgcc ttatacatca cctcgtcaat caatgccgac
     4021 ggcggcagcc acgatttccc gccttttcct tccacacgca gggaaaacag ctcggcaatg
     4081 cccagcatcg cggaaatgca aaacgcgccg taaccgataa agtttgccgg aacgtggatt
     4141 ttcatccacc aagattgcaa tgccggaatc agcggctgga tggtatgcgc ctcgcgggac
     4201 acgctgtacc acaagacaaa tccaaccacg accgccataa agccgaacac gaagccgccc
     4261 aatttctgta tggcgaactt accttcataa taaagataca tcagcgcggt aatcaccaaa
     4321 aacaggatga acacttcata caggttggaa accggaatat gccccgcatc gggacggagc
     4381 agatagcttt cgtgccaacg taccagcaga ccggtaaagc ctgctacggc agacacccat
     4441 gcaaacacgg ttcccatacc caacagcgtg ttggtcggca cattttttac gcttgccaaa
     4501 accgcgcccg aaatataggc gaacagggcg aaaaagacaa aggcgcactg ccacataatc
     4561 gccgactggc tgctgaggaa ataccgcaac aggaaaatct ctgccgattt gatgttgccg
     4621 ccatacagac cgacggcggc ataggcaagc aatacgctta aaggaacaaa ccagcgcatc
     4681 ggtttgaaaa accaacccaa aaacacggca ataccggcac tcgcccacaa catgaccgtt
     4741 tcgtaaatgt ccatatgcat accggaacgg gtctgtacga aaaccgtagc cgcaaaaacc
     4801 agcacggcaa atacccaatc ccaaagattc agattgctga tcaaagactt ctgaatcagc
     4861 agctcgtgtt ccggaagggt tttatagtgt tcagtcatga ttcaagtcct tgccgagccg
     4921 ttgcagactc tcgacgtgtt ttggaaattc cttctgcaaa tcccgttcgc tgcgggccga
     4981 agacatggca aaacggattt tgccgtctga aaacaatacc cacgcccgtt tttcgcgcac
     5041 ataaaacatc aataccgtac ccaataccaa cagcaccgag ccgagataga ccaaaagcgc
     5101 acccggggaa cgggtcatct gcaaacccga cgaacgcacc tcggaaaacc cgtcaagttg
     5161 cagcagcata ggcgcgggat attcggtcaa acccgtgtac gcatccatac tgtgcaacag
     5221 gaaacgattc cgcgcttcat cctgctgcca ttcgggcaag ccgtaccggc gtatggtttc
     5281 atccaaagca gcgttcatca cgccgtaaag catttcgtag aaatagccct gcatcttatc
     5341 ctgctgctct ttcgggatat tggacgtaat aaattcgtcc aatcccaaat agcctttttg
     5401 tgcaaagatg ttcagcgtgt tttccgcagc cagcatgaat tgttcgcgga tttcggcagg
     5461 tgcgcctttg gttgcgtcgg caaccagacg tttgcgccct tccccatctt tcaaaaactc
     5521 acgcaatgcc ataaaggtgt ccgctttcaa ctgcttgtcc aaggggatac gcagccagcg
     5581 gtattgctgc tgcaagccgc tgcgcgtgcc ggtaatccaa aaataatcct gttcctgcaa
     5641 aaccggcagc atatagtttt tatattcgac cgcctgccct gccgcatcac ggatacggta
     5701 aacaatggaa gggccgatat tggtgtattt tttaccttcc tgagtaacgg cgcggacatc
     5761 gttcagcgtg gatttcaggc ttttttcccg ttccgcgccc tcgctcatgt cctccacatt
     5821 catagaagtg aactgatcga actcaagacg atatttgtgt ttgccaattt ccaacggaaa
     5881 ctgccgcata gaggttgcct tcaacacgac aggctcgcgc gaagcatcac ccaaattcca
     5941 cgccttgaat gtcaaatccg aaccgccgtc ggcaaaactc gcctgataaa tcgtgatgcc
     6001 gtgcaaggtc aaaggatggt tcacgcggat ggtgcgctcg agtttctcac cggttgcctt
     6061 atccgttact tcaatatcac tggcaaaatc gcgcggcata ccggtattgt aaaagtcgat
     6121 atgaaatttt ttcagtttga cttcaaaagg caagtcctga accaacatcc cgttgtcggc
     6181 actcaggaaa accacatccg cactttgccc ctcggaaata ttgacgttgc ccctaaatga
     6241 gagattggac gcacccaaaa tactttcggg cttgaaatcc ttggcataaa ccgcctgatt
     6301 gtccggaaca atccgaccgg tcagcatacc cagcttcaac agcaggttac tgtctatcaa
     6361 cccgcccagg caaatgacaa tcaaagcaac atgggcaaag atatagcccc atttgttcat
     6421 cgtgcctttt ttggcggcaa tcagaaccga cccgtcctca cggctgacgg tttttccctg
     6481 aaaaccctgc acctccagat aacgtttggc aacttcgggg gcaattttta catccaacag
     6541 cgaagaatgg cgcatcgccg ccagagattt ttctttaacc ttttcccgaa aagacttcat
     6601 ttcgcgccag aacggcggca cattgcgaat caggcacaaa ctggtagaaa ccaccagaaa
     6661 catcatgata acgacaaacc atgccgaagc atagacgtca tacagtccca gaaaaccaaa
     6721 aatctgcgcc caaaaaggtc cgaatttgac caaataatcc gtctgcggct ggttttgctg
     6781 caacaccgta ccgataaccg atgcaatacc cagcagactg agcaaagcga ccgcaaagcg
     6841 catggagctg aaaaaagcga accacggacg ggaaagaagt gggggagatc tacgggattt
     6901 actcattgtg tgtttattcc gccatcagga atatgggaaa gcagaattgg gcaaacagaa
     6961 aacaacgtcc cgattctact gtcttgatgc tttttatttc aagacaatga agacagcctg
     7021 catcgattcc aacggttgcg attgaaaaaa cttatcgcag aattgcctga agccgtctga
     7081 aaacttttca gacggcctct aaaacagact attgcggaat taacgcaaac cttggataaa
     7141 gttggcgacc gctttcaaat cttcttcaga catacggttt gcaatatctt ccatgatggt
     7201 atttttacgc tgaccggact tgtaggcatt catctgttca acaatatatg cctgatgctg
     7261 accgcccaaa cgcggataag cctgaatttc gcttccgcct cccggcatac ccgcaccgct
     7321 cggaccgtgg caggacatac acgccggcac ttttttatcg ctcaaaccgc cgcgatagat
     7381 tttcgcaccc aattcgggat tttccttagg attggcttca ccggatttgg gctgctgttt
     7441 ggcatagaat gcagatacgt tcaaaatatc ctgatcgctc aaattcatta ccaccggttt
     7501 catcacagct gccgaaccgt gggtgcgttt accgtcgcgg atgccgatag tttgatgata
     7561 gatgtaagca gtatgctgtg ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc
     7621 tgctgcatgg caagccgcac aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc
     7681 tttgggagag gcggaaaccg caccggcagc caaaacaaag gccaataaag tcaatcgttt
     7741 catggagtgc tcctgattac agcattggat aacgcaacaa tgctcttttt atattcaaat
     7801 acgggatttt tgacccgatt aaaaccgatg attctgtaaa cgtgttattc tatactaaat
     7861 ttacattaaa ttaccactgt gtttcacata aaaccaaccg catatttttg ctgtcggaca
     7921 aacggcggcg gaaaacaagg atatgcccat gaaccttttt caaaacgcca aattcttcac
     7981 gacggtcaac caccttaaag acctgcccga cacccctctc gaaattgcct ttgtcggcag
     8041 gagcaatgcc ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc gtcttgccta
     8101 cgtttcaaaa acacccggac ggacgcagca tatcaacttc ttcgagctgc agaacggcaa
     8161 ttttatggtc gatttgcccg gctacggtta tgcccaagtc cctgaagcag tacgcgcaca
     8221 ttgggtcaat ctgctcggcg actatctgca acagcgcaaa cagcttatcg ggctggtttt
     8281 gattatggat gcccgccatc ctttaaaaga actcgacatc cgtatgctgg attttttcca
     8341 cacgaccggc agaccggttc acatcctgct gtcaaaagcc gacaaattat ccaaaaacga
     8401 acagataaaa accctgtccc aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa
     8461 catcagcgta cagctgtttt ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac
     8521 tgtcggaagc tggtttgacg cagcagatgc cgccgcttcc tctccagagg aaaactgacc
     8581 ccaattatac ggaaaccgta tttcccccac ttgaccgacc gcaaacattt aaaaaattgc
     8641 cactgccaaa tctaaaatgc cgtctgaaag acttttcaga cggcattttg cggagtcttt
     8701 aaaacagaga atccaactgc tgctgtttgg aaccagtatt actcggaagc accggcgttt
     8761 cctgcatatc ttggcggact tcgtcatccg ccgcctgccg tccgccttct gccgcgcccc
     8821 cgtcatcttc ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg tccagcgtca
     8881 agcccggatc ggttaccata cgttccttca tatagtattc gccattgctg ctgaccacac
     8941 cttcaggcat tttcatcccc ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca
     9001 cccaaaccgg caccgcaatc gtaccgccgt agccgacacg ccccatactc ttaggtttgt
     9061 cgaagccgat atatacggca gtaaccacat cagggttaaa accgacaaac cacgcatcct
     9121 tattgtcatt ggtcgtaccc gttttaccgg caatatccgt tcttcccaac gcagctgccc
     9181 cccttgccgt accgacacgg accacatcct gcataatctt atacataata taggcattgc
     9241 gcggatcgat tgcctgaggc gcattttgcc cggccaccaa aggttgcatt tgggcgcgca
     9301 acctgccgtc tctgtcataa atcttatcga ttacgtgcga agaaacccta tatccgccgt
     9361 tcgcaaatac gctatatgcc tccgccactt tcaacggcgt tgtctcgccc gtacctaaag
     9421 ccatagacag gcttgccggc agctcggacg acctgaagcc gaaacgccgg atatactgtt
     9481 gcgcgtaacc gacaccgata gacatcaaaa tacggatgga aaccatattc ttggaagccg
     9541 tcagagcctg tctcaaagta atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc
     9601 aaaccgaacc gttcggccct ttccccggca gggaaatcgg cgcatcgtta accactgtgg
     9661 acgcggtcat ccccttagat aatgccgccg aatagacaaa cggcttaaag gtcgaacccg
     9721 gctgccgcat tgcctgaacg gcacgattga atgttttgct gtgaaaatca taaccgccga
     9781 ccagcgcgcg cacagctccg gtttttgcat ccagcgaaac caaagccccc tgcagcaacg
     9841 gctcttgaac caccgcccaa cgcccgccgt tgtttttgac acggatgacc gcgcccctgc
     9901 ggatacggtc ctcccccatt ttttcattat tgaccgcgcg ggccgcaaaa cccaaggcgc
     9961 gcctgtcaag cgtaacccgc ctgccgccgg gcagctgtat gacgacattt ttctttttag
    10021 tcacatccaa cacaacggcg ggaaccattt tatcgacggt atagagtccc gacagatact
    10081 ggctgacagt ctcctcgaca tcttcactct tactcaaatc gatatagttt tccgcaccgc
    10141 ggtagctgct gccgcgatcg aaattccgta gagccttgcg caatgcctcg gttgccacct
    10201 tctgatgatc ggcgcggacc gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac
    10261 cgtatttctc atacagttcc tgacgcacca tttccgccac atataaagca ctctgatcga
    10321 ttttccgaac aaaccgctcg taatgcagtt cctcattcaa cgcctgatcg cgctgttgca
    10381 cggtaatcat cttctcctcg agcatattgt tcaaaatata cttctggcgc aacttggcac
    10441 gttccggatt aacaatcgga ttataggcag acggagcctt gggcagtccc gcaagcatgg
    10501 cggcttccgc caaagtcaaa tctcggacat tcttattgaa atagatttgc gcggcagatg
    10561 caaaaccata ggcgcgctga ccgaggtaaa tctgattgaa atacaactcg aggattttgt
    10621 ctttgcttaa agactgctcg attttatagg caagcaacac ctcattgaat ttgcgtgtga
    10681 acgttttttc actgctcaaa taaaaatttt tcgccacctg ctgcgtaatc gtactcgcac
    10741 ccgactgcac gctgccggac acgacattgc cgacggcagc gcgggcaaca ccccaaacat
    10801 ccacccccca atgccggtaa aagcgtttgt cctcggcggc gataaccgca ttccgcaaca
    10861 cctctgggaa atcgccgatt tttgtaaatt cgcgccgctg ctccccatac ataccgatga
    10921 cttccccatc cgccgaataa atagtcaacg gcattttagg ctggtaatgc tgcaaagaat
    10981 ccaaagacgg cagtttcgga tacgttacca aaatagcaat ggcaaccaaa cccactccaa
    11041 atacacaaaa cccaaaaacc aaaccaaaac aagtcgttaa aatcttttta atcatagctg
    11101 aataataatt taccattatt ggtattaaat aaagtaaaat agcaaccgat ttctacaaag
    11161 cacggtttca atgtgcaaag aacaaggaat ccattacgga taccgaaacg gttactcact
    11221 gtacaaataa agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa
    11281 aaacagatgc caagctccct aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca
    11341 tcgatatcga ccagcattcc atcaaaatgg tccaattgtc aggacgtagt ttaaaccaaa
    11401 ttcaattgga aaaatacgtc attgccaaat taccaaagaa tatcattcaa agcaataaag
    11461 tccaaaatta cgatcaactt gttacatatt tgcaacaagc ctatgccaaa ctgggtactt
    11521 cgtgcaaaaa catcatcgcg tccgtcccgc aaaatctggc aaccatcgaa caattgacct
    11581 acacagacaa agatgcagaa ttagacctgc aggggttcgt ggagtcctcc atctccgaag
    11641 tcagctcgat atcgctcgaa gaagccaatt acgactatca ggtcttgtcc caatcggccg
    11701 ccggcgaagc tgtgttggcc gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg
    11761 cattcaacgc cgccggtatg aaattatccg cgcttgatgt ggacattttc ggacaataca
    11821 acgcctacgc gctatggata aaccatttcg cccccgagct tgcagccgaa aaagtcgcca
    11881 ttttcggcgt atatgccgca cagacctacg ccttggtcat ccaagacgga aaaatcctat
    11941 acaaacagga aacctccgtc agcgaagaac agctcaacca actcatccag cgcacctatc
    12001 aggtaacaga agaaaaagcg gaagaaatca tcaactcccc gcaaaaacct tccgattacc
    12061 aagaaagcgt ggcaaactat ttcaaccagc agattaccca agaaatacaa agggtcttgc
    12121 agttttatta caccacgcag accgcagacg atatgaccga catcaagcat atcctgctga
    12181 ccggggaagc ggcgcgccag gaaggtatcg cccaaaccgt cgcctcacaa accaatgcag
    12241 atgtacaatg cgtccatccc gcgcgttatt ttgcggacaa cctcaaaaca gacaaacaac
    12301 aattcgaact tgatgcgccg acactgacca gggcgttcgg tttggcggta cggggattat
    12361 aattatgaac aatttaatca aaatcaacct cctcccctac agggaagaga tgaacaagcg
    12421 caaacagcag cagtttaaaa cgctgatgta cggtgccgtg ctgacgggcg ttgccgccgt
    12481 tgcggcaacc tacctgttta tcgacaatat gatcaataac cagtcggaaa gaaacacgct
    12541 gctggaaacc tccatcgcac acttggatac cgagctgtcg gaaatacaaa agctcaaaca
    12601 ggaaaaagat gccttcctga ttaagaaaaa caaaatcgag gagctccagc tcaaacgcct
    12661 ccaagccgca aaaatcctcg acagcctgaa tgaggccgtc cccggaagca cctacctgac
    12721 ctcgctggat gccgttaccg ccgactctta tcggctcagc ggcaggacat ccagcgacaa
    12781 ccgcgttgcc gccatgatga gggcgatgcc caataccggc atattcaagc aacccgaatt
    12841 gttaagcatc aagaaaaaca attcgcatca agaatttacc cttcaggcaa cattacaacc
    12901 catcgtaaag gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa
    12961 ctgaatggct tctaaatcat ctaaaaccaa cttggatctc aacaaccttc acctgctcaa
    13021 ccttcctgcc aggcttttta tcgccctgct ggccgttgcc gccgtgctgg ggctcggtta
    13081 tgccggattg ttcaaaagcc agatggaatc ccttgaggaa tacgaagcaa aagaaaccga
    13141 actgaaaaac acctacaaac agaaaagtat cgacgcggcc agcctgaaca acctgaggga
    13201 cgaacttgcc tcaatccgct ctgccttcga tatcatgttg aaacagctgc cgacagatgc
    13261 agaaattccc aatttggttc aagagcttca tcaggcaggt tcgagcaacg gcctgcgctt
    13321 ggacagcgtt atgccccaac ctcccgtaga tgacggcccc atcaaaagat taccctattc
    13381 catttccatt accggaaatt acgaacagat cagccaattt acccgcgatg tcggcagcct
    13441 ctcccgaatc attacccttg agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa
    13501 tcctgacggc aagagcagca tcctgaacct cagcgccatt gccaccacct accaagcaaa
    13561 atccgtagaa gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt
    13621 agggaaacca tgaaacacta tgccttactc atcagctttc tggctctctc cgcgtgttcc
    13681 caaggttctg aggacctaaa cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa
    13741 atcatacctt tccaagcacc taccctgccg gttgcgccgg tatacagccc gccgcagctt
    13801 acagggccga acgcattcga cttccgccgc atggaaaccg acaaaaaagg ggaaaatgcc
    13861 cccgacacca agcgtattaa agaaacgctg gaaaaattca gtttggaaaa tatgcgttat
    13921 gtcggcattt tgaagtccgg acagaaagtc tccggcttca tcgaggctga aggttatgtc
    13981 tacactgtcg gtgtcggcaa ctatttggga caaaactacg gtagaatcga aagcattacc
    14041 gacgacagca tcgtcctgaa cgagctgata gaagacagca cgggcaattg ggtttcccgt
    14101 aaagcagaac tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc agcacctgcc
    14161 gcagaacaaa attaagaaga ggattactcc attatgaata ccaaactgac aaaaatcatt
    14221 tccggtctct ttgtcgcaac cgccgccttt cagacagcat cggcaggaaa cattacagac
    14281 atcaaagttt cctccctgcc caacaaacag aaaatcgtca aagtcagctt tgacaaagag
    14341 attgtcaacc cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa
    14401 caaaccggca tttccatgga tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa
    14461 atcagtgccg cacaaaacag cagccgtgcg cgtctggttc tgaatctgaa caaaccgggc
    14521 caatacaata ccgaagtacg cgggaacaaa gtttggatat tcattaacga atcggacgat
    14581 accgtgtccg cccccgcacg ccccgccgta aaagccgcgc ctgccgcacc ggcaaaacaa
    14641 caggctgccg caccgtctac caagtccgca gtatccgtat ccgaaccctt taccccggca
    14701 aaacaacagg ctgccgcacc gtttaccgag tccgtagtat ccgtatccgc accgttcagc
    14761 ccggcaaaac aacaggcggc ggcatcagca aaacaacagg cggcagcacc agcaaaacaa
    14821 caggcggcag caccagcaaa acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc
    14881 ggcattatcg aattggctgc attgggcttt gccgggcagc ccgacatcag ccaacagcac
    14941 gaccacatca tcgttacgct gaaaaaccat accctgccga ccacgctcca acgcagtttg
    15001 gatgtggcag actttaaaac accggttcaa aaggttacgc tgaaacgcct caataacgac
    15061 acccagctga ttatcacaac agccggcaac tgggaactcg tcaacaaatc cgccgcgccc
    15121 ggatacttta ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac
    15181 aatgcgccca aaaccttcac aggccggaaa atctcccttg acttccaaga tgtcgaaatc
    15241 cgcaccatcc tgcagatttt ggcaaaagaa tccgggatga acattgttgc cagcgactcc
    15301 gtcaacggca aaatgaccct ctccctcaaa gacgtacctt gggatcaggc tttggatttg
    15361 gttatgcagg cacgcaacct cgatatgcgc caacaaggga acatcgtcaa catcgcgccc
    15421 cgcgacgagc tgcttgccaa agacaaagcc ttcttacagg cggaaaaaga cattgccgat
    15481 ctgggcgcgc tgtattcaca aaacttccaa ttgaaataca aaaatgtgga agaattccgc
    15541 agcatcctgc gtttggacaa tgccgacaca accggaaacc gcaatacgct tgtcagcggc
    15601 aggggcagcg tgctgatcga tcccgccacc aataccctga ttgttaccga tacccgcagc
    15661 gtcatcgaaa aattccgcaa actgattgac gaattggacg tacccgcgca acaagtgatg
    15721 attgaggcgc gtatcgtcga agcggcagac ggcttctcgc gcgatttggg cgttaaattc
    15781 ggcgcgacag gcaagaaaaa gctgaaaaat gatacaagcg cattcggctg gggggtaaac
    15841 tccggcttcg gcggcgacga taaatggggg gccgaaacca aaatcaacct gccgattacc
    15901 gctgccgcaa acagcatttc gctggtgcgc gcgatttcct ccggtgcctt gaatttggaa
    15961 ttgtccgcat ccgaatcgct ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc
    16021 caaaaccgca aagaggccaa aatcgaatct ggttacgaaa ttcctttcac cgtaacctca
    16081 atcgcgaacg gcggcagcag cacgaacacg gaactcaaaa aagccgtctt ggggctgacc
    16141 gttacgccga acatcacgcc cgacggccaa atcattatga ccgtcaaaat caacaaggac
    16201 tcgcctgcgc aatgtgcctc cggtaatcag acgattctgt gtatttcgac caaaaacctg
    16261 aatacgcagg ctatggttga aaacggcggc acattgattg tcggcggtat ttatgaagaa
    16321 gacaacggta atacgctgac caaagtcccc ctgttgggcg acatccccgt tatcggcaac
    16381 ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt cattaccccg
    16441 aggattatgg gtacggccgg caacagcctg cgctattgat gcgtcaaaat aagggcatat
    16501 gttttacggc atatgccctt tctttatgct ttttgccgcg accgaaatgc cgtcattccc
    16561 gcgcaggcgg gaatccagtc cgttcagttt cggtcatttc tgataaattc ctgttgcttt
    16621 tcatttctgg attcccactt ttgtgggaat gacggcggaa ggggtaaatc ctcgcaatcc
    16681 aaagcctcgt catttccaca aaaaacagca acccggaaca gcaacttaaa accccgtcat
    16741 tcccgcgcag gcaggaatcc aacttgtccg gttccggttg tttttcgtct cgtaactttt
    16801 gagccgtcat tcccgcgcag gcgggaatcc agtccgttca gtttcggtca gtttcggtca
    16861 tttccgataa attcctgttg cttttcattt ctggattccc acttttgtgg gaatgacggc
    16921 ggaaggggta aatcctcaca acccaaagcc tcgtcatttc cacaaaaaac agcaacccga
    16981 aacagcaact taaaaccccg tcattcccgc gcaggcggga atctaggtct gtcggttcag
    17041 gaacttatcg gataaaacgg tttctccaac cctgcgttct agattcccac tttcgtggga
    17101 atgacgggat atgggtttcc gtgcggacgt gttcggattt ccgcctgcgc gggaatgacg
    17161 gc
//
LOCUS       DAXIEB010000036        17064 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000036 DAXIEB010000000
VERSION     DAXIEB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.036"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(17..1855)
                     /gene="glmS"
                     /locus_tag="ACFOA3_000965"
     CDS             complement(17..1855)
                     /gene="glmS"
                     /locus_tag="ACFOA3_000965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6897990.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            2137..2655
                     /locus_tag="ACFOA3_000966"
     CDS             2137..2655
                     /locus_tag="ACFOA3_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6897991.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
     gene            complement(3089..4414)
                     /locus_tag="ACFOA3_000967"
     CDS             complement(3089..4414)
                     /locus_tag="ACFOA3_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6897992.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            4636..5475
                     /gene="efeU"
                     /locus_tag="ACFOA3_000968"
     CDS             4636..5475
                     /gene="efeU"
                     /locus_tag="ACFOA3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6897993.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            5472..6638
                     /gene="efeO"
                     /locus_tag="ACFOA3_000969"
     CDS             5472..6638
                     /gene="efeO"
                     /locus_tag="ACFOA3_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6897994.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            6902..8167
                     /gene="efeB"
                     /locus_tag="ACFOA3_000970"
     CDS             6902..8167
                     /gene="efeB"
                     /locus_tag="ACFOA3_000970"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6897995.1"
                     /translation="MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(8239..8568)
                     /locus_tag="ACFOA3_000971"
     CDS             complement(8239..8568)
                     /locus_tag="ACFOA3_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6897996.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8644..10014)
                     /gene="glmU"
                     /locus_tag="ACFOA3_000972"
     CDS             complement(8644..10014)
                     /gene="glmU"
                     /locus_tag="ACFOA3_000972"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6897997.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDANTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLSDDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(10069..10341)
                     /locus_tag="ACFOA3_000973"
     CDS             complement(10069..10341)
                     /locus_tag="ACFOA3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6897998.1"
                     /translation="MRKTFLILTAAAALLSGCAWETYQDGNGKTAVRQKYPVGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTGNNADEESRQHWQKPKFQNR"
     gene            complement(10367..11047)
                     /locus_tag="ACFOA3_000974"
     CDS             complement(10367..11047)
                     /locus_tag="ACFOA3_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6897999.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPKTQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFAALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKAFGMKTVWFGAKSHALPFIDTCARDMAQLARYAETLSER
                     RQNHYNTP"
     gene            11106..12107
                     /locus_tag="ACFOA3_000975"
     CDS             11106..12107
                     /locus_tag="ACFOA3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6898000.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            12201..13049
                     /locus_tag="ACFOA3_000976"
     CDS             12201..13049
                     /locus_tag="ACFOA3_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6898001.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            13076..13561
                     /locus_tag="ACFOA3_000977"
     CDS             13076..13561
                     /locus_tag="ACFOA3_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6898002.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(13663..15231)
                     /gene="msrAB"
                     /locus_tag="ACFOA3_000978"
     CDS             complement(13663..15231)
                     /gene="msrAB"
                     /locus_tag="ACFOA3_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6898003.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRIEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            15377..16642
                     /gene="ftsY"
                     /locus_tag="ACFOA3_000979"
     CDS             15377..16642
                     /gene="ftsY"
                     /locus_tag="ACFOA3_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6898004.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         3672 a         4348 c         5129 g         3915 t
ORIGIN      
        1 cgcaaacaat ccggacttat tcgaccgtaa cggatttcgc caggttgcgc ggtttgtcta
       61 catcggtgcc gcgtgcgagg gcgacatggt aggaaaggag ctgcacgggg atagtatgca
      121 cgacggggga cagtttgccg acgtggcgcg gtgcgcggat aacgtgcaca ccttcggtgg
      181 cattaaaatt gctgtcgagg tcggcaaaga cgaaaagttc gccgccgcgc gcgccgactt
      241 cctgcatatt ggctttgact ttgtccaaca ggctgtcgtt gggtgcgatg acgacgacgg
      301 gcatgttttc gtccaccaag gcaagcggcc cgtgcttcag ttcgccggca ggataggctt
      361 cggcgtggat gtaggtgatt tccttcagtt ttaacgcgcc ttcgagggca atcgggtaat
      421 ggatgccgcg ccccaaaaac agcgcgctgg ttttcttggc aaactgttgc gcccaggcgg
      481 caatttgcgg ttcgaggttt aaagcgtgct gcacgctgcc gggaagctgg cggagttctt
      541 cggtgtaacg tgcttcgtct tcttcggaaa ccaaaccgcg cactttcgcc agcgttaccg
      601 ccaaaccgaa cagcgcaacc agctgcgtgg taaatgcttt ggttgaggcg acgccgattt
      661 ccgcaccggc acgggtataa agcacgaggc tgctttcgcg cggcagggcg gattccatca
      721 cgttgcagat ggagaggctg tggcggtgtc ccaaggattt tgcgtatttc aacgcctcca
      781 tcgtgtccag cgtttcgccg gattgggaaa tggtaatgac cagttgatcc ggatcggcaa
      841 tcacgctgcg gtatcggtat tcgctggcga tttcgacatc ggctgggatt ttggcgatgg
      901 attccaacca atatttggcg gtcagtgcgg cgtaatagga cgtgccgcag gcaaggattt
      961 tgacgctgcg gatgctttca aacacgcttt tggcgttttt gccgaagttt tcggggatga
     1021 agccgccgtc gaggaaaacc tccgccgtgt ctgcaatcgc gcgcggctgc tcgtggattt
     1081 ccttttgcat aaagtggctg tacggtccca gttccaaaga ggcgagcgag agttcggata
     1141 ccttgacttt gcgttcggca ggcaggccgt ttttatcggt cagccgtttg atgccgtctg
     1201 aagccagcag cgcgatgtcg ccgtcttcga ggtacgccac gcggcgcgta aaggcgatga
     1261 cggcggatac gtccgaagcg ataaaggttt catcgtcgcc caaagcgacc aaaagcgggc
     1321 agcccatacg cgccacaact aattcatcag gcttgtcttg ggcaataacc gcgatggcgt
     1381 atgcgccgtg gaaacgtttg accgcttctt gtaccgcttc aaacagcctg ccgccgtttt
     1441 gcgcgtattc gtgattgatg ctgtgtgcga tgacttcggt atccgtttgc gattcaaaac
     1501 ggtatcccaa accttccaaa cgtttgcgtt cgctttcaaa gttttcgatg atgccgttgt
     1561 gtacgaccgc aatcataccg ccgctgatgt gcgggtgggc gttcggctca gtaacgccgc
     1621 cgtgtgtcgc ccaacgcgta tgtccgatgc cgatgccgcc gctgatgcct ttttcgcgtg
     1681 ccgcgtcctc cataagctgc acgcgtccga cgcggcgcac acgtttgatt ttgccgtcgg
     1741 tgttgacggc aatgcctgat gagtcataac cccggtattc gaggcgttta aggccgtcgg
     1801 tcagaaaatc gacgacgttg tgatgggcgc ggatggcgcc gacgataccg cacataactg
     1861 ttccttagta tccggttgaa aaaaaacagg cgcggacggc ttccgtgccg caccttcctc
     1921 ttcggattat aaaccgcctc ccgcgccgga aaacagcaaa atgccgtctg aaggcttggg
     1981 cttgctcaaa aaagggaggg atttccccgc ctgaccgggg tgggcgttca gacggcattg
     2041 cctgctgctg gtttatagtt tttgcaaatc aatattgaca agctgaaaaa aaaaaacaat
     2101 atactcgctc ggtcttaatg ttaacggagt atggaaatga aacaaatgct tttagccgtc
     2161 ggcgtggtgg cagtgttggc gggctgcggc aaggatgccg gcggttacga gggttattgg
     2221 cgcgaaaagt cggacaaaaa agagggcgtg attgccgtca aaaaagaaaa aggcaattac
     2281 ttccttaata aaatcaacgt atttacaggc aaggaggagt ctttgctttt gtctgaaaaa
     2341 gacggcgcgc tttcgataaa cacggggata ggggaaatcc cgatcaaact ttccgacgac
     2401 gggaaagagc tgtatgtcga acgcaggcag tatgtcaaaa ccgatgcggc gatgaaggac
     2461 aaaatcatcg cccatcagaa aaagtgcgga caaacggcac aggcatacct cgacgcgcga
     2521 aaggcgttgc cgtcaaacca aacgtatcag cagcatcagg cggcgatcga gcagttgaaa
     2581 cggcggtttg aagccgagtt cgacgaattg gaaaaagaaa tcaaatgcaa cggcaaaccg
     2641 acattgttgt tttagcgtag gacaaccggg aggatgccgc cgtccgaatc ggatgtgtgg
     2701 ttttcgtacc ggtacgggcg ggcaggaatg tccgcttttt ttgttcggac gcgtttgaat
     2761 atccgtccga ttccgtccgt ttgcaaaggg tatttttgtt cgggcggaaa ttatagtgga
     2821 ttaacaaaaa ccagtacagc gttgcctcgc cttagctcaa agagaacgat tctctaaggt
     2881 gctgaagcac caagtgaatc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt
     2941 gtcctgattt ttgttaatcc actataattc cgtcaaataa gaaaggcgtt ttgtccgacc
     3001 ggccggcttg tgcctgcggt atcgcaaaac ttcgccttaa tgcgcccgat tgcctaggga
     3061 tgggcttcag atggcattgt tttccggttt acgggcggta ttcgggcttc ataccgttgg
     3121 gtaggagctg ccagacgtaa cccgtggttt tctgtttgcc ggcaagttcg ccggcttcgt
     3181 cgccgtatcc ccaaaaataa tccacgcgca ccgcgccttt aatcgcgctg ccggtatcct
     3241 gcgccataat caggcggttg agggctttgc gggtaaccgg atgggcggtg gcgacaaata
     3301 agggcgcacc caaggtaatg tagtgccggt cgactgcgcc ggcatattcc cccatcagcg
     3361 gcgtgcccag tgcgccgaca gggccgtcat tgccgcttcc ggtaagctcg cggaaaaaga
     3421 tatagctggg gttttgaccc aaaacttcgg cgaggcgttg cggattttgc cgcatatagg
     3481 ctttgatgcc ctgcatcgag gtttgtccga gtttgaggta gcccttatcc gccatatagc
     3541 gtccgatgga aacatacgga tgctcgtttt tgtcggcata accgatgcgg atgtatttgc
     3601 cggacggggt tttcagacgg ccggagcctt ggatgtgcat aaaaaagagt tcgacggggt
     3661 cttcggcgta accgagtatc ggtgctttgc cgtcaagcgc gccgccgttg atttggttgc
     3721 gcgtgtggta ggggaggaag cggcttcctt caaacctgcc tttgattgcc gttgtgcgcg
     3781 cggtgatggg gaatcgggag aggtcggcgg tgtgcgtgcc gccggcattg tcgattgtgc
     3841 cgctgttttt ccccgtctgc ctgatgcgga caagggcttt tccgctccgc aaaccggcag
     3901 gcagggggac ggagataaaa tcgtcgggaa taccgtaaat cgggaagcgg gcttgtgccg
     3961 tccgcctgtc gtcgcccttc agcaccggtt cgtaatagcc ggtaaccgta ccggcaaggc
     4021 ttccgttgcc tgcaacctgc cacggcgtga aatagcgttc aaaaaactgt tttgcctgaa
     4081 aggaatggac gggggtttga aaggcttggg cgcacacatc ctgccagcct tggcggtttt
     4141 tcaaattggc gcagccgagg cggaaggatt gcaggctttt ggcgaaatcc tgcgccgccc
     4201 agtggggcag ggacaggtgc ggtacaacgg tataaacggc cccgccgccg ccgaccgtcg
     4261 ttccggcggg gtcggggatg ccgaccggcc ggtccgggcc gttgatgacg gatgtgtcgg
     4321 gttgcggaaa ggtttggatg ctcttgcttt ggcaggcggc gaggatggcg gcggcgatgc
     4381 cgtacagggc ggcgcggaat aggtattttt tcataatgga caatgttgcc ggcagtaata
     4441 aggcagtaat aagaaagatg gtttcgggcg gcgttgcggc agccgtggag aggggatttt
     4501 aacacagggc gcagctgcag cctgcggaac tttccgccgc gcggtactgc agataaaaat
     4561 aacttgcatt tgtatttaca agcaatgaaa atattccgat aatatattat tcatcatcct
     4621 tgttcgttcg cgtttatgct ggtcgctttt ttaattatgt tgcgcgaggg tatcgaagcc
     4681 gcgctcattg tcggcatcgt tgccggtttt ctgaaacagt ccggacattc caaactgatg
     4741 cctaaggtct ggttcggggt cgtccttgct tctttgatgt gtttggggct ggggtacggc
     4801 atccattcgg caacgggcga gattccccaa aagcagcagg agttcgtcgt cggcattatc
     4861 ggcctggttg ccgtcgcgat gctgacttat atggttttat ggatgaaaaa ggcggcgcgt
     4921 tcgatgaagc agcagcttca ggattctgtg caggcggctt tgaaccgggg cagcggacaa
     4981 ggatgggcct tggtcggtat ggcgtttctt gccgtggcgc gcgaaggttt ggagagcgtt
     5041 ttttttctgc ttgccgtatt caaacagagc ccgacttggc agatgccggc cggtgcggta
     5101 gcgggggttt tggctgccgc cgtgattggc gcgttgattt atcagggtgg gatgcgcctg
     5161 aatctggcga agtttttccg ttggacgggg gcgtttctga ttgtcgttgc cgccggcctg
     5221 cttgccggct cgctgcgcgc gctgcacgaa gcaggcattt ggaacgcgct tcaggacatt
     5281 gtgttcgact catcaaaata tttgcacgaa gacagtccgt taggtgtgct gctcggcgga
     5341 tttttcggct ataccgacca tccgacgcag ggcgaggcct tggtttggct gctgtacctt
     5401 attcccgtca tgacttggtt tttgtgcggc agcaggccgt ctgaaacttt aacccgtaaa
     5461 gaggagctga aatgagaaaa ttcaatttga ccgcattgtc cgtgatgctt gccttaggtt
     5521 tgaccgcgtg ccagccgccg gaggcggaga aagctgcgcc ggccgcgtcc ggtgaggcgc
     5581 aaaccgccaa cgagggcggt tcggtcagta tcgccgtcaa cgacaatgcc tgcgaaccga
     5641 tggaactgac cgtgccgagc ggacaggttg tgttcaatat taaaaacaac agcggccgca
     5701 agctcgaatg ggaaatcctg aaaggcgtga tggtggtgga cgagcgcgaa aacatcgccc
     5761 ccggactttc cgataaaatg accgtcaccc tgttgccggg cgaatacgaa atgacttgcg
     5821 gtcttttgac caatccgcgc ggcaagctgg tggtaaccga cagcggcttt aaagacaccg
     5881 ccaacgaagc ggatttggaa aaactgtccc aaccgctcgc cgactataaa gcctacgttc
     5941 aaggcgaggt taaagagctg gtggcgaaaa ccaaaacctt taccgaagcc gtcaaagcag
     6001 gcgacattga aaaggcgaaa tccctgtttg ccgacacccg cgtccattac gaacgcatcg
     6061 aaccgattgc cgagcttttc agcgaactcg accccgtcat cgatgcgcgt gaagacgact
     6121 tcaaagacgg cgcgaaagat gccggattta ccggcttcca ccgtatcgaa tacgcccttt
     6181 gggtggaaaa agacgtgtcc ggcgtgaagg aaattgcagc gaaactgatg accgatgtcg
     6241 aagccctgca aaaagaaatc gacgcattgg cgtttcctcc gggcaaggtg gtcggcggcg
     6301 cgtccgaact gattgaagaa gtggcgggca gtaaaatcag cggcgaagaa gaccggtaca
     6361 gccacaccga tttgagcgac ttccaagcca atgtggacgg atcgaaaaaa atcgtcgatt
     6421 tgttccgtcc gctgatcgag accaaaaaca aagccttgtt ggaaaaaacc gataccaact
     6481 tcaaacaggt caacgaaatt ctggcgaaat accggactaa agacggtttt gaaacctacg
     6541 acaagctggg cgaagccgac cgcaaagcgt tacaggcctc tattaacgcg cttgccgaag
     6601 accttgccca acttcgcggc atactcggct tgaaataagc cgcaagcgtt cagacggtat
     6661 ttggcggcag ataccgtctg aagttttata gtggattaac aaaaaccagt acggcattgc
     6721 ctcgccttgc cttgccgtac tatttatact gtctgcggct tcgtcgcctt gtcctgattt
     6781 ttgttaatcc actatatccg ccatatattg cagggcagga tttcaacctg ccgctatcgg
     6841 ttaatggaaa aacggcgtgc agggataccc atcctgctgc acggatattg aaggaaacac
     6901 catgagcaaa aaacaacccg cacaaccgac caggcgcact ctttttaaaa ccgcgatcgc
     6961 agccggagca gtcggcgcaa tcggaggtta tctcggcggc aaaaaacagg gcgaaaccgc
     7021 cgaacgcacc gccgaaagcc aacactcgcc ccaagcctat ccctgctacg gcgaacatca
     7081 ggcaggcatc gttacgccgc agcaggcgtt ttcgattatg tgcgccttcg acgtaaccgc
     7141 gcaaagtgcc aagcagctgg aaaacctgtt ccgcacgctg accgcccgca tcgagtttct
     7201 cacccaaggc ggcgaatacc aagacggcga cgacaaactt ccgccagccg gcagcggcat
     7261 tttgggcaaa gccttcaacc ccgacgggtt gaccgttacc gtgggggtgg gcagcagcct
     7321 gtttgacggc cggttcggac tcaaagacaa aaaaccgatt catttgcagg aaatgcgcga
     7381 cttctccaac gataagctgc aaaaaagctg gtgcgacggc gatttgagcc tgcaaatctg
     7441 tgccttcacc cccgaaacct gccaagccgc cctgcgcgac atcatcaaac acaccgccca
     7501 aaccgccgtt atccgctgga gtatcgacgg atggcagccc aaatccgaac ccggcgcgat
     7561 ggcggcgcgc aacctgttgg gcttcaggga cggcacaggc aaccccaaag tttccgaccc
     7621 caaaaccgcc gacgaggttt tgtggacggg tgtggccgcc aacagcctag acgaacctga
     7681 gtgggcgaaa aacggcagct atcaggcagt ccgccttatc cgccactttg tcgagttttg
     7741 ggacaggacg ccgcttcaag agcaaaccga cattttcggg cggcgcaaat acagcggtgc
     7801 gccgatggac ggcaaaaaag aagccgacca accggatttt gccaaagacc ccgagggtga
     7861 tatcacgccc aaagacagcc atatacgcct ggcgaatccg cgcgatcccg aattcctcaa
     7921 aaaacaccgc ctcttccgcc gcgcctacag ctattcgcgc ggacttgcct caagcggaca
     7981 gcttgatgtc gggctggtgt tcgtctgcta tcaggcaaac cttgccgacg gattcatctt
     8041 cgtgcaaaac ctccttaacg gcgaaccgct ggaagaatac atcagcccct tcggcggcgg
     8101 ctatttcttc gtcttgcccg gcgtggaaaa aggcggcttt ttggggcaag ggctgctggg
     8161 cgtataaatc cgccatataa aaaacgccgt ccgaaccttg ccaaacgggt tcggacggcg
     8221 tttcttgttt ttgggcggtc aggctttttt gacgaattcg gattttaaat tcatcgcgct
     8281 gccgtcgatt ttgcagccga tgttgtgatc gccttcctgc aggcgtatgc ctttgacttt
     8341 tgtaccttgt ttgatcacca tcgagctgcc ttttaccttg aggtctttga tgaggatgac
     8401 ggtatcgccg ttttgcagcg ccgcgccgtt ggcatcgcgc acttgagccg caaggtcggc
     8461 ggcggattcg gtttcattcc attcgtgggc gcattcgggg cagatgtatt gtccgccgtc
     8521 ttcataagtg tattcggagg cgcattgtgg gcatgggggt aatgacatag ttttccgtcc
     8581 ttatcggatg tttgtttttg ggatgccgtc tgaagcctga aaccggcttc agacggcata
     8641 gctttattgc ttgtcttttt cgggacgcac ccagccttcg atgacggttt ggcgggcgcg
     8701 ggcgagggcg agtttgttgt cttcgatatt gcgggtaatc gtgctgcctg cgcctgtggt
     8761 tactttgttg ccgagggtaa cgggggcgac taggacgcag tttgaaccga tgcggacttc
     8821 gtcgccgata acggttttgt gtttgtgcac accgtcgtag ttggcaataa tcgtacccgc
     8881 gccgaagttg gttttgctgc cgacttcggc atcgccgatg taggtgaggt ggttggcttt
     8941 ggtgcctttg ccgatggcgg cgtttttgat ttcgacgaag ttgccgacgt gtacgtcgtc
     9001 tgaaaggcgg gcttgcggac gcaggcgggc gtacgggccg attcggttgt tttcgccgac
     9061 ttcgcagctt tcgaggtggg agaagggggc gattttgctg tttgcgccga ttttggcgtt
     9121 tttgatgacg cagtttgcgc cgatttcgac gttgtcgccg agctcgatgt cgccttcaaa
     9181 gatacagttc acatcaatca cgacgtcttg cccgtgtttc agacggcctc gtaaatcgaa
     9241 acgtgccggg tcgtgcaggg taacgcctgc tttgagcaat tcttgcgcct gtccggtttg
     9301 gaagatgcgt tcgagttcgg cgagctggcg tttgttgttc acgccggcgg cgaggtggga
     9361 ggcgcggact tggacgggat gaactttaat accgtcggca acggctttgg cgatgaggtc
     9421 ggtcagatag tattcgcctt gggcattgtt gctggaaagg ctgttcagcc agttttccag
     9481 tttggcgttg ggcaggacga gaatgcctgt gttggtttct ttcacggctt tttgggcggt
     9541 attggcgtct ttttcttcga cgatggcggt tacgctgccg ttgccgtcgc ggatgatgcg
     9601 gcccaatcct gtcgggtcgg cgggcacgtc ggtcaacagc ccgacttcgt tgcctgcggc
     9661 ttcgagcagg gtttcgaggg tttcaacgtc aattaaagga acgtcgccgt acagcaccag
     9721 cgtgcggcct tcggcggaaa ggtggggcag ggcggttttg acggcgtggc cggtgccgag
     9781 ctgttcggtt tgttcgaccc aaacgacatc gcgtttgacg gtgtctaaaa cttgctcttt
     9841 gccgtggccg acgacgacgc agatgttttg cggattcagc gcggcggcgg tgtcgataac
     9901 gcgcccgagc atgggcttgc cgccgatgcg gtgcagcact tttggcattt tggaatacat
     9961 gcgcgtgcct ttgccggcgg cgaggatgac gatgtttaaa gtgttttgcg gcatgacggt
    10021 ttcctgtgca atgccgtctg aagcggcttc agacggcata gggtaggttt atcggttttg
    10081 aaactttggt ttttgccagt gttggcggga ctcttcgtcg gcgttgttgc cggtttggtc
    10141 gggcagcacg gcatggcgtt cggggcggta ttggttgtag ttcatatttt tcgagtagct
    10201 gccgtcttgg taataaacgg gcgtgccgac gggatatttt tgacggacgg cggttttgcc
    10261 gttgccgtct tgataagttt cccacgcgca gcccgacaaa agggcggcgg cagcggtcag
    10321 gataaggaag gttttacgca tggcttttct ttcgtatttt cgggggttag ggggtattgt
    10381 aatgattttg gcggcgttct gacaaagttt ctgcataccg agccagttgc gccatatcgc
    10441 gtgcgcaggt gtcgataaag ggcagcgcgt gggattttgc accgaaccag acggttttca
    10501 tgccaaatgc ttttgcctga tgcaggttgt ccgcgctgtc gtccaccata atgcagcatt
    10561 cgggcggtac gtccaacagg cggcagacat tgagatacgc ttgcggattg ggtttgtaca
    10621 gcagtccgaa atcatccgtg ccgaacagtg cggcgaaacg gttttccaaa ccgagtgcgc
    10681 cgacaacggc acggacgtaa aacgacgggc cgttggaaaa aaccgccttg cgccctttta
    10741 ggcggctcag ggtgttttgt gttttaggca tgccgtgcag cctagtcagg attgcatcga
    10801 tcggatggct ttcgcgcaaa aattcggcga tgtcgatttc gggatggtgg atttgcagtc
    10861 cggcgagcgt tgcgccgtag cggtgccaat agtcttgacg caggtcggac gcggcagatt
    10921 cggagagttt gaggcggcgt gccatatagc gtgtcatggc gcggttgatg agtgtgaaga
    10981 tgccggcatc ggcattgtgc agcgtgttgt cgaggtcgaa cagccacacg gtcgggtttt
    11041 cttgcatgtt gaaccgtgaa aatttgttag aatgttattt tacagcgaat agaggaggac
    11101 tcggaatgaa acggaaaatt tggctgctgc cgctgctggc ggtttcggca tacctgcagg
    11161 cgcagacgga agtcaggctg gcggtgcata agtcgttcag cctgcccaaa gggctgattg
    11221 cgcgcttcga gcgggcaaac gatgcgaagg tgtcgattat tcaggcgggc ggcgcgaacg
    11281 aaatgctcaa caaactgatt ttgagccgcg ccaacccgat tgccgacgcg gtgtacggtt
    11341 tggacaacgc caatatcggc aaggcgcggg aaatgggcat tttggcggca gcgcaacccg
    11401 aatccgcccc cgtcgcggtc gggctgcctt cggctttggc ggtcgattac ggctatgtgt
    11461 ccatcaatta cgacaaaaaa tggtttgaag gcaaaaagct gcccctgccg caaaccctgc
    11521 aggatttgac ccgccccgaa tataaaaacc tattggtcgt gccgtctccc gccacgtcgt
    11581 ccccggggct gggcttcctg atggcgaaca tcagcggtct gggcgaagaa ggcgcgttca
    11641 aatggtgggc acagatgcgg cagaacggcg tgaaggtcgc caaaggctgg agcgaggcgt
    11701 attacaccga cttttcgcac aacggcggcg cgtatccgct ggtggtcggt tatgccgcca
    11761 gcccggcggc ggaagtgtat ttttccaaag gcaaatacag cgagccgccg acgggcaacc
    11821 tgtttttaaa aggcggcgta ttccgccagg tcgaaggcgc ggcagtcttg aagggcgcga
    11881 aacagccgaa gctggcggca aaactggtgc aatggctgca aagtcgggaa gtgcagcagg
    11941 cggttccgtc cgaaatgtgg gtttaccccg ccgtcaaaaa cacgcgcctg cccgacgtgt
    12001 tccgcttcgc ccaagccccg acgcacacca ccgcccccgc gcagcgcgat attgatgcga
    12061 accagcgcgg atgggtttcc cgttggatta gaacggtttt gaaataaaac aaacataccg
    12121 ccccgcaggg cttcatacgg catttttaca cctgtgccga ttacgccgca cggggcggat
    12181 gttcgatcaa gaggaaaaca atggacttca aacaatttga ttttttacac ctgatcagtg
    12241 tttccggttg ggagcatctg gctgaaaagg cgtgggcgtt cgggctgaac cttgccgccg
    12301 cgctgcttat ttttttggtc ggaaaatggg cggcgaaacg cattgtcgcc gtgatgaggg
    12361 cggcgatgac gcgcgcgcag gtcgatgcca cgctgattag ttttttgtgt aatgttgcca
    12421 atatcggctt attgattttg gtgattattg ccgcattggg cagattgggc gtttccacaa
    12481 catccgtaac cgccttaatc ggcggcgcgg gtttggcggt ggcgttgtcc ttgaaagacc
    12541 agctgtccaa ttttgccgcc ggtgcgctga ttatcctgtt tcgcccgttc aaagtcggcg
    12601 attttatccg cgtcggcggt tttgaaggat atgtccgaga gattaaaatg gtgcagactt
    12661 ctttgcggac gaccgacaac gaagaagtcg tgctgcccaa cagcgtggtg atgggcaaca
    12721 gcatcgtcaa ccgttccacc ctgccgcttt gccgcgccca agtgatagtc ggcgtcgatt
    12781 acaactgcga tttgaaagtg gcgaaagagg cggtgttgaa agccgccgtc gaacacccct
    12841 tgagcgttca aaacgaagag cggcaggccg ccgcctacat caccgccttg ggcgacaatg
    12901 ccatcgaaat cacattatgg gcttgggcaa acgaagcaga ccgctggacg ctgcaatgcg
    12961 atttgaacga acaagtggtc gaaaacctcc gcaaagtcaa tatcaacatc ccgttcccgc
    13021 aacgcgacat acacatcatc aattcttaaa cgccgtctga aaggggagtg ggaaaatgga
    13081 cgcgctgcac accatcgccc gaaacctgac gaaaaaacgt caaaccgtaa gctgtgccga
    13141 gtcctgtacg ggcggaatgc ttgccgccgc attcacaagc gttgcaggca gttcgcaatg
    13201 gttcgaccag agttttgtaa catacagcaa caaagccaaa gaagaccgct tgggcgtgtt
    13261 gcccgaaacc ctgctcgaac acggcgcggt cagccgccaa accgtctatg agatggcgcg
    13321 cggcgcgaaa gccgtggcgc aggcggatta cgccgtcggt atttccggca tcgccggacc
    13381 gggcggcggc agcgaaagca aacccgtcgg cacggtttgg ttcgggtttg cctttccggg
    13441 cggaagttgc gaagcaatgc gccgttttga cggcaaccgc gaatccgtcc gcgcgcaggc
    13501 ggtcgccttc gcgttggaac ggttggcggg gctgattgaa aacggcggcg atgctgtcta
    13561 aacaaaatct ccgtctggac agaatccccg ccggataaaa aataccgtct gaaacgttgc
    13621 aggtttcaga cggtattttg tcgggttagg cggcggtgcg gcttatttca ctttactctt
    13681 caacgcgccg tagcctgccg cgtccatttg ttccagcggg atgaatttca agctcgcgcc
    13741 gttgatgcag tagcgcagtc cgcctttgtc gcgcgggccg tcggggaaga cgtgtcccaa
    13801 atgcgaatcg gcggcgtggc tgcgcacttc gatgcggcgc atattgaagc tgaaatcatc
    13861 gtgttcggta acggattttg catcaatcgg gcgcgtgaag ctcggccagc cgcagccgga
    13921 atcatatttg tcggcggagc tgaacaaagg ttcgccgctg acaacgtcca cataaatgcc
    13981 gggtttgaac aaatggtcgt attcgtggct gaaggcatat tcggtcgcgc tgttttgggt
    14041 aacttggtat tgctcttcgg tcagggtgcg tttgagttcg gcgtcactcg gttttttata
    14101 cgttgccgcg tcgaagcctt tgccttgcgg tgcggtcttg gttttgcccg gcagcggttc
    14161 gtcggctttg cggatatcga tgtggcagta gccgttgggg tttttaatca agtagtcctg
    14221 atggtattcc tcggcatcgt agaagttttt cagcggctcg ttttcaacaa cgaggggcag
    14281 ttggtatttt tgctgctcgc gtttgagggc ggcggcgatg acggcttttt cggcggggtc
    14341 ggtgtagtac acgccgctgc ggtattgcgt accggtgtcg ttgccctgtt tgttgaggct
    14401 ggtcggatca acgacgcgga agaaatattg caggatgtcg tctaggctga gtttgtcggc
    14461 atcgtaggtc actttgacgg tttcggcgtg gcccgtatgg cggtaggaca cgtcttcata
    14521 gctcggattt ttcgtgttgc cgttggcgta gccggatacc gcgtcaacca cgccgtcgat
    14581 gcgttggaaa taggcttcca agccccagaa gcagccgccg gcgaggtaaa tggtgcgcgt
    14641 gttcatgatt tttgaatcct ttttctgagt gtcgggtttg tagaacgaat gtttcaagct
    14701 gcccaaatcg gcattcgggt cgcggattaa cgccaacgcc tgcgcttcgt tgatgctgcc
    14761 tttgacgatg cgctgcacgt cgccgtcttt accgattaac gcccacgagg ggtaaacgct
    14821 gatattcagg ctttgggcga tcgtgccgcc gttgtcggtt acgacgggca gcttgggata
    14881 attcaaaccg gcataccatt tttggaagtc gccgtctttt ttctcgtgca aaaagcccgg
    14941 agacgcgacg gtaatcaggt tggcggagct gaattttgca tcttgcgccc atttttcggt
    15001 ctgtcccaat tcggacaaac acaaaggaca ccagctcgcc caaaatttaa tcagcgtcgg
    15061 tttgtctttt ttcaagtaaa cactggcggg gcggttgtct gcggttttca aagtggataa
    15121 agtgtgcggc acggtcgcgg ctccggcatc gacgattttg ggcgaacaag cacccagcgc
    15181 aagcaggcag ccgaacttgg cgcaaaggga aaagaaagta cggtgtttca ttttaatgtt
    15241 tcctgtgtgg acggtttgca tgattagacg tttgagatgc cgaaacctta cagctcggat
    15301 tttcagacag ccttgccgcg taaaatacgc tacaatacgc cctatttcaa gtttctaaaa
    15361 ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag aaaaaacagg aaacgccggc
    15421 tcccgaggag gctcaagttc aggaaactgc agcaaaagca gaatccgaag ttactcaaat
    15481 agttgaaaat attaaagaag aagtcgaatc tttaacggaa agcgtcaaag ggcaggtcga
    15541 atcggctgtt gaaaccgtca gcggtgcggt tgaacaagta aaggaagccg ttgccgagat
    15601 gctgtctgaa gcagaggaag cggcggaaaa agcagcggaa caagtcgaag cggcaaaaga
    15661 agccattgcc gaaaccgtcg gcgaggctgt cgggcaagtt caagaagccg ttgcaacaac
    15721 tgaagaacac aagctcggtt gggcggcgcg tttgaaacaa ggtttgacca aatcgcgcga
    15781 caaaatggcg aaatcgctgg cgggcgtgtt cggcggcgga caaatcgacg aagatttgta
    15841 cgaagagctg gaaaccgtgc tgattaccag cgacatgggc atggaagcca ccgaatacct
    15901 gatgaaagac gtgcgcgacc gcgtcagcct caaagggctg aaagacggca acgaattgcg
    15961 cggcgcgctg aaagaagcct tgtacgacct gattaagcct ctagagaaac cgctggtttt
    16021 gcccgaaacc aaagagccgt ttgtcatcat gcttgctggc atcaacggcg cgggcaaaac
    16081 cacgtctatc ggcaaactcg ccaaatattt ccaagcgcag ggcaaatccg tattgctggc
    16141 ggcaggcgac actttccgcg ccgccgcccg tgagcagctt caggcatggg gcgaacgcaa
    16201 caacgttacc gtgatttcgc aaaccacggg cgattccgcc gccgtgtgct tcgatgccgt
    16261 ccaagccgcc aaagcgcgcg gcatcgacat cgtgcttgcc gacaccgccg gccgcctgcc
    16321 cacgcagctt catttgatgg aagaaatcaa aaaagtgaaa cgcgtgctgc aaaaagccat
    16381 gcccgacgcg ccgcacgaaa tcatcgtcgt gcttgatgcc aacatcgggc aaaacgccgt
    16441 caaccaagtc aaagcctttg acgacgcatt agggctgacc ggtttaatcg tgaccaaact
    16501 cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgcc tccgaccgcc ccgtccccgt
    16561 ccgctacatc ggcgtgggcg aaggcataga cgatttgcgc ccgtttgacg cgcgcgcgtt
    16621 tgtggacgca ctgctggatt gagccgaaat gccgtccgaa aacagcagac cgatgccgtc
    16681 attcccgcgc aggcgggaat ctagaccttg ggataacggc aatattcaaa ggttagttga
    16741 agctttagag attctggatt cccactttcg tgggaatgac gggatgtagg ttcgtgggaa
    16801 tgacgcggtg caggtttctg tgcggatgga ttcgtcattc ccgcgcaggc gggaatccag
    16861 accgttgggc atctgcagcg gtttgctaaa aaacgcttta ccgtgatcag tgtgcaaagt
    16921 taaaatgggg aagtaagctt ttcaatcagc aattcggcgg gcgcggaatc gggcggttta
    16981 ccgaaccccg gcgttcgcgg cgcgccgccc cgtcccgtga aggcaaactc aaggaataaa
    17041 agatgaataa aacttggaaa cggc
//
LOCUS       DAXIEB010000037        17021 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000037 DAXIEB010000000
VERSION     DAXIEB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.037"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(27..653)
                     /locus_tag="ACFOA3_000980"
     CDS             complement(27..653)
                     /locus_tag="ACFOA3_000980"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6898005.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="ACFOA3_000981"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="ACFOA3_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6898006.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="ACFOA3_000982"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="ACFOA3_000982"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6898007.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRRGVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DADDEE"
     gene            complement(3056..3553)
                     /locus_tag="ACFOA3_000983"
     CDS             complement(3056..3553)
                     /locus_tag="ACFOA3_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6898008.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTIPFFVSLSEAVLKHADDRDG
                     ALEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="ACFOA3_000984"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="ACFOA3_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6898009.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="ACFOA3_000985"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="ACFOA3_000985"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6898010.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGNTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="ACFOA3_000986"
     CDS             complement(5863..6309)
                     /locus_tag="ACFOA3_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6898011.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="ACFOA3_000987"
     CDS             complement(6314..6673)
                     /locus_tag="ACFOA3_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898012.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="ACFOA3_000988"
     CDS             complement(6746..7474)
                     /locus_tag="ACFOA3_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6898013.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="ACFOA3_000989"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="ACFOA3_000989"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6898014.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8623..9312
                     /locus_tag="ACFOA3_000990"
     CDS             8623..9312
                     /locus_tag="ACFOA3_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6898015.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            9497..10699
                     /gene="trpB"
                     /locus_tag="ACFOA3_000991"
     CDS             9497..10699
                     /gene="trpB"
                     /locus_tag="ACFOA3_000991"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6898016.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(10878..16346)
                     /gene="iga"
                     /locus_tag="ACFOA3_000992"
     CDS             complement(10878..16346)
                     /gene="iga"
                     /locus_tag="ACFOA3_000992"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6898017.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYKPHKAWNASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGKEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADEIKQRDNAGTIKG
                     YGEHHWKTTGTNSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFF
                     KGDYTVKGINNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALN
                     SFGATQINGNVNLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHY
                     QLHVQDKTGEPNQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLY
                     NPYAENRRRVKPAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAK
                     AEQVKRQQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAA
                     ELSAKQKVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEE
                     RRQTAQSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEE
                     RKAAELLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKA
                     AAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQE
                     ARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKR
                     RAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPL
                     DALEDENVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQ
                     DNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSI
                     LLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRS
                     LSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSR
                     LQTQQKANFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSS
                     MSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQQYS
                     AGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         3457 a         4171 c         4623 g         4770 t
ORIGIN      
        1 tcattccagc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcgactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacgtcg
      121 accgcttccg ctccggtgat gcggacggct tcgccgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccagcagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg gtggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtcgaact ggcggcagaa tgtgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggcacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg atttttttgg cgcgggcaat atcgacggcc
      541 cggctgctgc cttggaaaaa gaccagcccg acggcatctg cacctgccgc tacggcggcc
      601 gatgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacggc gcacttgcac cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgttcaaaac
      781 cacttcgtcg ccttcgcgcg ctttaatcag gcaacgcgcc aacggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacaggccga cggtcgcgcc gaaaaatact tggtcggtcg cttcgcgcaa
      961 ttcgggatcg acgacgacgg cggactccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag atagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttgttga ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgtccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcgga ttgagtgctt tgacgacggc
     1501 ttccaaatcg cccaagtctt gaaatacgat gccgtccgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgcccgttgg caatcaactc ttcgcgcgtg ggcatatcaa tgccgtacac
     1621 attgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgcccgccgc
     1681 gcgtaccatt tcgacgattt cgcggctggt cgtcccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctgaccgg gcatgataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 gccctcgcgg tagggtttgt cgaggtggac ggcaagctcc atcgcgctgg ggcggctggt
     1921 gtcgggaatg ggcatcacga catcgatgtc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccaaggac acgcccatat ccaagcgcga ttggtaaacc gatacgccgt cgattacgga
     2041 gtcggggcgg gcaaaataaa catattcaaa aaggcagggg ctgagtttgg cacggtcgct
     2101 gcattggcgg gcaatcattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 gtcgcgttcc aaatcgtagg caagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggatttcctg ccttcgttgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacgccgt atgcgccgcg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactc cgcggcgtaa ttcgtgcgcg aatacgttga gcaaaacttc
     2461 ggaatcggag ctggtgttga cgtggcgcag gtgtttgttg cacacgtttt cataaagttc
     2521 ggccgtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaaaggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgccggc gttgcccgcc gtgggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgcgtg cggaacactt cgcgcaccat
     2701 ccctttgcct ttgtgcatat ggaaggtgcc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagaggat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccaa agctccatcc ctgtcgtccg catgctttaa cactgcttcg gaaagcgaca
     3121 cgaaaaacgg tatggtatag gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttt
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca agcattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tattgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgctgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaggtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ctgtctgaaa
     6301 ggcgcgcacg gcgttacatc atattgtcga acacgggcgt gatgttcaaa tccgcttctt
     6361 ccatatccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttgtcga tgtcccagcc ggcgagttcg gcttcaaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggttcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggctat gccgtctgaa
     6721 cgttcggttt tcagacggta tagcatcagt gggtcatgac ctgttgcagg aattgtttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat ggtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgctgct gctggccgcc ggaaagttgg gacggaaaag
     7081 cgtctttttt gtgcgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctccgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt gatgttttcc aatacggtca
     7201 ggtgggggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcgtca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gccacatatt tctccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tgctcgaaat cgtccgcctt gccgatacgg
     7861 tgtttcaccg gaatcatgcg gacgacggcc gaatccactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgatgatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttccttttc ggttgaaacc ccgcccttta
     8461 gggcggtaga atcagactct atttgggagg ggcgtaactc cttccaaatc aggatggcac
     8521 atagggcggt gctttaagtg tcgtcctgtg tgttggaaca taaatgtgtt tacagtatcc
     8581 gtttgatgtc ggcattgtaa ccgaaaacgg cagggcgtga taatgctgtt tgaaggcttg
     8641 ccgtgtttgg cggtttggtg caaaaaccgg ctgtctgccg ttttgcctgt tggaggattg
     8701 aacgtgtctg aaaatctgct tgaaatcgaa acccatccct tcgattccgt gttgccgccg
     8761 aaggctgcgg tcatgatgat ggggacgttt ccgcccaagg aagacaaacg cgcgatgcag
     8821 tttcattatc cgaatttcca aaacgatatg tggcgcgttt acgggctggt gttttttaat
     8881 gatgcggcgc atttccaaag gttgtctgaa aaagcgtttg atgccgagaa aatcaaggcg
     8941 tttttgcacg aacgggggat tgcgtcctgt ccgaccgtgt tgaaggcggc aagggaacac
     9001 ggcaatgcgt ccgacaagtt tttaaaggta gtcgaaaccg tcgatttggc ggcggtgttg
     9061 gcgaaaatac cagactgccg ccatatttgt acgacaggcg gcaaggcgac ggaaatcctg
     9121 ctcgatattc agggtggcgg catcaaaatg ccgaaaacgg gcgaaaccgt gccgtttccg
     9181 tttgccggac gggatttgac gctgacgcgc ctgccttcga cttcgcgtgc ctatcctttg
     9241 agtttggcga aaaaagcggc ggcgtatcgg gcgttttttg aaatggcggg cttgtgtgaa
     9301 aaacagttat aattgccgac aatttcccgt tcggacggca tgtttgaaaa atatagtgga
     9361 ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt acaaatagta cggcaaggcg
     9421 gggcaacgcc gtaccggttt ttgttaatcc actataaatt gccgtctgaa aatttgaagc
     9481 acaaggacga aatctgatga agaactacca cgcgcccgac gagaagggct ttttcggcga
     9541 acacggcggg ctgttcgtgt ccgagaccct gattccggct ttgcaagagc tggcggatgc
     9601 ctataaggca gcgaaaaacg atcctgaatt ttgggaagcg ttccgccatg atttgaaaca
     9661 ttatgtcggc aggcccagcc ccgtttacca cgccgcgcgg ttgtccgaac atctgggcgg
     9721 cgcgcaaatc tggttgaagc gcgaagactt gaaccacacc ggcgcgcaca aagtcaacaa
     9781 caccatcggt caggcactgt tggcaaaacg catgggtaaa aaacgcgtca tcgccgaaac
     9841 cggcgcgggt cagcacggcg tggcgagtgc caccgttgcc gcacgcttcg gtatgacttg
     9901 cgacgtgtat atgggcgcgg acgacatcca acgccaaatg cccaacgtgt tccgtatgaa
     9961 attattgggt gcgaacgtgg tcggtgtaga aagcggcagc cgcacgctga aagacgcgat
    10021 gaacgaagcc atgcgcgaat gggtcgcccg cgtggacgac acgttctaca tcatcggtac
    10081 cgccgccggc cccgcgccgt atcccgaaat ggtgcgcgat ttccaatgcg tgattggcaa
    10141 cgaagctaaa gcgcagatgc aggaagccat cggcagacag cccgacgttg ccgttgcctg
    10201 cgtgggcggc ggatcgaacg ccatcggttt gttccacccg tatatcggcg aagaaaacgt
    10261 gcgcctcgtc ggcgtggagg ctggcggttt gggcgtgaac acccccgatc acgccgcgcc
    10321 gattacttcg ggcgcaccga ttggcgtatt gcacggtttc cgcagctatc tgatgcagga
    10381 cgaaaacggt caggttttgg gtacgcactc tgtttccgca ggcttggatt accccggcat
    10441 cggcccggaa cacagccatc tgcacgacat caagcgcgtc gaatacactg ttgccaaaga
    10501 cgacgaagca ctcgaagcct ttgacttgct ctgccgcttc gagggcatca tccccgcgct
    10561 cgaatccagc cacgccgttg cttgggcggt gaaaaacgcg ccgaaaatgg gcaaagacca
    10621 agtgattctg gtcaacctat ccggtcgtgg cgacaaagac atcaataccg tcgcgaagct
    10681 caaagggatt aaactgtaac ctcgtccgtc tgatatagtg gattaacaaa aaccagtacg
    10741 gcgttgcctc gccttgccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    10801 ttttgttaat ccactataaa aatgccgtct gaagcctgag ttcagacggc attttatttt
    10861 gctatgaatt tagtatttta gaaacgaatc tgtattttaa tttgtccgga tttttgtttt
    10921 tccaattgtt ttccttttgt aatactgcca tttacgttta atgtaacatt acggtacagt
    10981 aacgcggcac ctgctgaata ttgctgttga ttatctgctt tataggcgaa ggaattaccg
    11041 cccacattca cgccaccttt gccataattg gcaaagtaag ctgcagataa caagggtttt
    11101 acggtaaggt tgccgacttt aaaccgataa gcaaaatcca gtccggccgt cagtgttttc
    11161 actgacatag aacttacttt aacactgtcg ttacccaact tgtaatctgc agatgacagg
    11221 cggctgtaac ggatccccgc actggggaca atctcgaatt gattgatttt cagcgtattg
    11281 cccaaagtaa ggccggtttg gatgcttgct cggttaaagt ttgctttttg ctgcgtttgt
    11341 aaccggcttc tcaagctgcc tgcaccaata tcgccggcca cataccaagc atcatttaaa
    11401 taatacttac cataaaggtt ggcttgcaca aaagtatttt tgccgctcgc ctgatcaaaa
    11461 gtatgctgac tgtcagagta agtcaatacg ccgcctatct gcatattttc ggacaagctg
    11521 cggtcaatgc cgatttgtgt ttgcgtgcgt ttcgaactaa accggcgata ttgtgcggaa
    11581 gcataatcac ggccataacc gatgttcgac atccaaacgc tgtttttttc ggcatcagca
    11641 cgtgattttt gtgcaatgtg ccgtgttaat gaagcacctg tatccaacaa gatagattgc
    11701 gtgcttgcca ttgcgtcaga taaagccgag ttggtattgg tgctgactgc atcggcttgc
    11761 gcgacggctt gggcttgcgg ctgcgcggcg gcttgggctc gcggctgagc ggctcttggt
    11821 tgcaaactca ctgtctcaac tttttcatga agttccgtat tgtcttgagg ctgtttgtct
    11881 gatgtgtcaa ccgattcgga tacatcttca tcttccaacg catccaaggg gatttcttca
    11941 taatcatttt catatttttc atgaagttcc gtattgtctt gaggctgttt gtctgatgtg
    12001 tcaactgatt cagatacatt ttcatcttcc aacgcatcca aggggatttc ttcatagtca
    12061 ttttcatacc agtccggatt atgcaaggcc ctgggtgctg cgtaagctga cgaatcaaat
    12121 gatggcgagg gcggtgccgg cagagtagat ctgcgtccgc gacgtttcgg acgattttgg
    12181 gaagctgcca tataatcctg aggtgcggca cggcgttttc ggcgttgcgg ctgggattgg
    12241 gctgcttgac ggtgctcttc ttcctcagct tttcgtttcg cggattctgc cgcttctcga
    12301 tctgtttcgg ctttttgttt tgccgacaac tctgctgctt ggcgttccgc ttcttgacga
    12361 cgtgcaagtt cggcggtctg gcgagcttct tgctggcgtt ttgctgcttc ggctgctgcc
    12421 ctttgcttgg caagttcggc ggttttgcgt tcggtttccg ctttttgttt gacggctaac
    12481 tctgcagctt ttcgtgcttc ttcctgttga cgcgcaaggg ctttttgttg gcgtgccgcc
    12541 aatgcttggg cttcagctgc ggctttttgg tttgccgaca attctgccgc tttgcgttct
    12601 gcttcctggc gatgtgcaag ttcggcagct tggcgttttg cctcttcggc ttctgctttt
    12661 cggcgcgcag ccaatgcttg agcctcacgt tcggcttctg ccctttgttt tgccaacaac
    12721 tctgccgctt tgcgttcttc ctgctgtcgg cgcgcaagtt ctgcttgggc ttgggcaatt
    12781 tccacattgt tttgctgtac cgaacggtgt ccgcggcgtt taggacggtc ttgggaagct
    12841 gccatataat cctgcggtgc ggcacggcgt ttgcggcgtt gcggctggga ttgggctgtt
    12901 tgacggcgtt cctcttcccc gaccctttgt tttgccgaca actctgccgc ttcgcgttct
    12961 ttttcatgcc ggcgtgcaag ctctgcagct tggcgttttg cctcttcggc ttctgctttt
    13021 cggcgtaccg ccaatgcttg agcctcacgt tcggcttcga ctttttgttt tgccgacaac
    13081 tctgccgctt cgcgttcttt ttcatgtcga cgtgcaagtt cggcgctgct gcgttcttgt
    13141 tctgcttttt ggcgggtcgc cagctctctt gcttcgcgtt cggcttctgc tttttgtttt
    13201 gctaactctg ccgatttacg ctctgcttct gcttgctgac gcttcacttg ctccgctttt
    13261 gcttgttggc gttttgcttc ttcggcttga tttgcctgcg ggctaggcgg tgcgacgacg
    13321 atattttgag gcttggcaat ttgtgcactg tccgtttgtg cttgagaagc cgtgtttgtg
    13381 gcaggagacg gggccggttt gactcggcgg cggttctcgg cataaggatt gtacaatcgg
    13441 gtaatgccat tttctgtttt gattgtataa cgcaatgcgc ctaaatctac atggttattc
    13501 gccaaggaaa cggaaaggtg cgatcggtct cgtacggatg atgcatcaaa gagattcagc
    13561 ccttcctgat tgggttcgcc tgttttatct tgaacatgga gctgataatg accggatgcg
    13621 gattccttca caagcacttt atccccaaga ttttttgcca agtgcgtcag ataatgaaaa
    13681 tgcccgttac cggataaatg attgattttg agcgtgtggt atttatttgc actttgcgcg
    13741 tcggaagcgt tgttcaaatg aatatggcta tccgccaatg acagattgtg tacttggctg
    13801 tcgccggtca aatgccattt gctatgttga tttaggctga cacggctgtt tccttgccct
    13861 tgaatttgcc cccataatgc agccttgccc aaagtcaatg ccgcattctg actcaaattc
    13921 acattgccgt taatctgtgt cgctccaaag ctgtttaaag ccttatcgga taagttgtct
    13981 gtgttgcagg taacgtaacc ggtatagtcc gagcgcacgc aaacctcatc gccgtttttg
    14041 taacccaaat ttactttggc gttgtctgtt gcggtgatgt tggcggtgat gtcggataca
    14101 tttctgccgg aagagaatga tgcggattga ttaaccgcaa tttctgtggc tttgaatgtg
    14161 cggtttatcc agtcgtcttc aaatacgact tcattgtttt tggagaaatg tgcgtctttt
    14221 cgggctgaag atttgttcac aaaatctctt gcgtgtggtg ttggacgacc tgataacaag
    14281 acattgcctt gagttacgct tatttttccg tttaaattag tgccgcctgt taacaagaaa
    14341 cggttttgcg cgcttttgcc gttgaaatta aggtttaatg caccgttatg tccttttccg
    14401 ttttcttcgc caaagaaacc gctaaaaccg ctaatacgct gattgttttt gtggttcatc
    14461 gcgtttttct tggcttcttc ttgtgtgctg cccattaaaa tccagtcgtt attttccgtt
    14521 tgtccgtttt cgggcatcgg tgcgttcacg ctgccgccgg attttagggc gtaataacga
    14581 tagtttttgt aataaagatc tttgccttgt gggatgggtc gccttggtcg gtaataataa
    14641 ctataatcat cttcatcata gtcattttgt atactatgta ccgaaaggct gttcggattg
    14701 gtaattaaag atttacccgt tagcgtgatt gtggaggtgt ggcctgtgtt gtggttgaca
    14761 atgcgcgcgc cttcatccac gttgcggatg tgttcaaaag tcaagtcatt gccattggca
    14821 tccaaacgac cgccacggaa accgaaatat aggttatcgg gattaatctg atttgaacta
    14881 tttaatacca atgtaccgcg tccgctgaca atgccgactt gggagaaagc ctggactttt
    14941 tggtttgaat cggctttttg attcagaata accgtaccgt cgccgacttt taattgccct
    15001 tggttaacgc ctgtgccgtt tatttctaat gtgcctttgc cgattttcgc caatctgtcg
    15061 ccattcggat ttttgacttg ccaaacgact tttttgccgt cggcaacatc aatccccgca
    15121 cctagccaag tgatgtcatt atttatacct ttgactgtgt aatcgccttt gaaaaacaga
    15181 ccgcccgcgc cttggttgat gttttgatcc aataccaaag tgccgttgtt ttcaaaggta
    15241 acattctgtc cgttgtttgc acccctttca ttgccggcaa gccttaccgc tgtcgaaccg
    15301 atatggctgt ttgtgcccgt ggttttccaa tgatgttcgc cataaccttt gatggtgccg
    15361 gcgttatcgc gttgtttgat ttcatctgca aattcttttt tatagatatt ccattcttgc
    15421 caagattttt ttccgtaacc tgcccaataa tcataagttc ccaaaaagac ccattgattt
    15481 ttttgtttgt caaaagcaaa taacggagag ccgctatcgc ccaatacgcc gtaatttgtt
    15541 aacgcatctt gcgagagtgc ttgtttaagt tcttctgccg aatactgttt attatgatta
    15601 ccaaagccaa tcaaaccttc ggtattcatt gtttggtcaa tattaatatc tttataaggt
    15661 gtacctgcaa tggcataacg ataggcttgt gaaagatcgc gcaaatcgta gcctttttcc
    15721 tttccttctt gatgataagc ccctttttca taaactaatt gcctgcctgc gccaactctg
    15781 acaaaagagg aaaaacggtt tttatctttg taggtatcca atccgccgcc ggcatcagtt
    15841 ggtgcaattg gcgcaacttc agttacaaac ttgttaaaac gcgccatatt ataatcttcg
    15901 agacggccta agttgctcgc attccaagct ttatggggtt tatagttatt ttgttcgaca
    15961 acgcggtatt cattttcttt gtcggctaca tcattatgac cgttgtattg accgtaataa
    16021 aaagtatgga cttctgcttt ggcgtgtttg acgctgacgg catattgggg atcgactacc
    16081 gttgctatgc gtttgttgac atctgcaacg ctaaaatcaa tcatcggtac gttggataat
    16141 gcgttgccga tgttttgacc ttgtttgttt ttcactgata aatcggttgc gccgacaaaa
    16201 aatttgcctt tgttttctgc aaagtcacgg aatatttgat aatcgacatc gtctctgacc
    16261 aatgccgctt ctgagtatgg cgtaagggca taggcaagaa agatggataa ggatatggcg
    16321 ttaattttaa aacgtttggt tttcataagg ttttaccgtt ttaagagtga tagtgttttg
    16381 tattttacgc cggtttggtg gtgttgacta ttttttgttg tgtaaatcat tcattttggt
    16441 gttttattgc caatttaaaa aaagaatccc gatgttttta tttccgcttc ctttgttctg
    16501 ttattcaagc gaaagcgggg agccggtttt cggggttcgg ttcttccgtt aaatttctgc
    16561 ggctttttgt ttttggattc ccgcttgtgc gggaatgacg ggattttagg tttctgattt
    16621 tggttttctg tttttgaggg aatgacggga tgtaggtttt cttaaccctg cgtcctagat
    16681 tcccgctttc gtggtaatga cgggatgtgg gttcgtggga atgacgcggt gcaggtttcc
    16741 gtacggatgg attcgtcatt cccgcgcagg cgggaatcca gaccttagaa caacagcaat
    16801 attcaaagat tatctgaaag tccgagattc tggattccca ctttcgtggg aatgacggga
    16861 ttttaggttt ctgattttgg ttttctgttt ttgtaggaat gatgaaattt tgagttttag
    16921 gaatttatcg gaaaaacagc aaccgctccg ccgtcattcc cgcgcaggcg ggaatccaga
    16981 ccttaaaggc agcggcaata ttcaaagatt atctgaaagt c
//
LOCUS       DAXIEB010000038        16711 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000038 DAXIEB010000000
VERSION     DAXIEB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.038"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(91..426)
                     /locus_tag="ACFOA3_000993"
     CDS             complement(91..426)
                     /locus_tag="ACFOA3_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6898018.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(506..2635)
                     /gene="dnaX"
                     /locus_tag="ACFOA3_000994"
     CDS             complement(506..2635)
                     /gene="dnaX"
                     /locus_tag="ACFOA3_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6898019.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMISAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQGNNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETGASLSEMPSENPIQTTPNDEAVETEAF
                     AHEAPAEPFYGYGFPDNDYPVENGAEIPPPDWEHAAPADTAGEGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLARAYGLQLTLQTQDWQDEVGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQVFGAQWQPESLELAANRP"
     misc_feature    complement(1407..1446)
                     /gene="dnaX"
                     /locus_tag="ACFOA3_000994"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2730..4706)
                     /gene="mutL"
                     /locus_tag="ACFOA3_000995"
     CDS             complement(2730..4706)
                     /gene="mutL"
                     /locus_tag="ACFOA3_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6898020.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPIGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMIERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGEDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHALNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENNSENLFDRASNYPTGNKPDIRNAFVSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELSQFEQARFGNMPSETPAQKT
                     DMPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAACAAPAML
                     GKSDVVSLAKDVLNEFAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4850..5035
                     /locus_tag="ACFOA3_000996"
     CDS             4850..5035
                     /locus_tag="ACFOA3_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6898021.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5046..5714
                     /locus_tag="ACFOA3_000997"
     CDS             5046..5714
                     /locus_tag="ACFOA3_000997"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6898022.1"
                     /translation="MTTNLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            5714..6430
                     /locus_tag="ACFOA3_000998"
     CDS             5714..6430
                     /locus_tag="ACFOA3_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6898023.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            6478..6963
                     /gene="purE"
                     /locus_tag="ACFOA3_000999"
     CDS             6478..6963
                     /gene="purE"
                     /locus_tag="ACFOA3_000999"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6898024.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7146..8600)
                     /locus_tag="ACFOA3_001000"
     CDS             complement(7146..8600)
                     /locus_tag="ACFOA3_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6898025.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8597..9298)
                     /locus_tag="ACFOA3_001001"
     CDS             complement(8597..9298)
                     /locus_tag="ACFOA3_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6898026.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9295..10074)
                     /locus_tag="ACFOA3_001002"
     CDS             complement(9295..10074)
                     /locus_tag="ACFOA3_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6898027.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10358..11794
                     /locus_tag="ACFOA3_001003"
     CDS             10358..11794
                     /locus_tag="ACFOA3_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6898028.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTF
                     RLGLAGNLFSRLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLM
                     KRFGYRTVLLWNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTL
                     ADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSI
                     GGITLASSLVFKRLHVSDGANLTRNTPS"
     gene            11906..13432
                     /locus_tag="ACFOA3_001004"
     CDS             11906..13432
                     /locus_tag="ACFOA3_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6898029.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVSEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            13641..14174
                     /locus_tag="ACFOA3_001005"
     CDS             13641..14174
                     /locus_tag="ACFOA3_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6898030.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMATASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14212..15513)
                     /gene="aroA"
                     /locus_tag="ACFOA3_001006"
     CDS             complement(14212..15513)
                     /gene="aroA"
                     /locus_tag="ACFOA3_001006"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6898031.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVARMHERPIGDLTDALRIAGTDVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTRQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FAHELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            15908..16384
                     /gene="greA"
                     /locus_tag="ACFOA3_001007"
     CDS             15908..16384
                     /gene="greA"
                     /locus_tag="ACFOA3_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6898032.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3517 a         4510 c         4651 g         4033 t
ORIGIN      
        1 agacccgtcg gcacggaaat ttatcgagta aaacggtttc ttagattcta cgttctagat
       61 tcccgcctgc gtgggaatga cggttgggta tcagcggaag aagtcgccca ctccgggggg
      121 tagaccttgc gtgaatgcgc ccattgtttt gtttgcggtt tcttcggctt tgcctcgggc
      181 ggatttgagg gcggcgagga tgaggtcttc aagcatttct ttgtcgtcgg cggcttcttg
      241 aatcaaatcg gggctgatgt cgattttgcg tacttcgtgc gcgcaggtca ttgtgatttt
      301 gaccaggccg ttgcctgctt cgccttcgat ttcggtttcg gcgagtttgg cttgcgcttt
      361 tttcatattt tcctgcattt gctgcgcctg tttcatcagg ccgcctaatc cggcttttcc
      421 gaacatactg aatactcctg tctgttttga ttgaatgagg gaaacggcaa tgccgttcga
      481 agggttcggg cggcattata tctgtttatg gccggtttgc cgccaattcc agtgattcgg
      541 gctgccattg cgcgccgaat acttggagga ttttttgtgc ggcggggtcg gcttcgagca
      601 atgcttgtgc tttttgcctg tcttcggctt ggacgcgctt gtcctgcatc gcgggggttt
      661 ctctgccgac ttcgtcctgc cagtcttggg tttgcagggt gagttgcagc ccgtaggctc
      721 gggcaagggt gtcgcggatt ttgtcgagcc gctttttgtc ggcggtggcg cgcgcttcgg
      781 cggtcatcgc caaaaccatc agaccggtgt cgggatggta ttccgtccac gcggagtgtt
      841 gtgccggcat ttgcgccgcg ccgagtttgc gggcgaagtg ccggacgatg gctgcccagt
      901 tttcggtgga aaattcgggc ggcggggcgg acggtgtgtt gtttccgccg atgccgcctg
      961 cttctgcttc ttcgtccgcg ccttctcctg cggtatcggc aggggcggcg tgttcccaat
     1021 ccggcggcgg gatttctgcg ccgttttcta cggggtagtc attatccgga aagccgtaac
     1081 cgtagaaagg ttctgcaggg gcttcgtgtg caaatgcttc tgtctcaacg gcttcatcat
     1141 tcggtgttgt ctgaatgggg ttttcagacg gcatttcgga taaggaagcc ccggtttcgt
     1201 tgtcggctgc cttgtttttg gaaacttggt tttcgggcgg cgtttcggct tcggatgccg
     1261 tctgaatgct ttttgccgac gtttgcgccg tgccggctgc ggcttgggct tcgtctggcg
     1321 cgtcttccca aggcggaaca tcgttgtttc cttgattgga aacgggttcg gcagttttgc
     1381 tttcagacgg cattgccgct gcggatgccg tctgaacgga tgtttgggcg gtttccgttt
     1441 cagggcgcgg ttggggcttt tttgcggcgg tttccttttc ggcggtttgt gccgatgggg
     1501 attttagttc ggtattttca atcacggcat ttgcatcaca cgatgctgct gccaagggcg
     1561 caaacgccag catacgcagc agggtcatca taaagccggc gtattcgtcg ggggcgaggc
     1621 tcaggtcgcg tttgccgtgg acggcgattt ggtagtaaag ctggatttgt tcgccgccta
     1681 tggtttgggc gaggcggtgc aaaatatcgg aatcggggtc gtcgtgcgcc aaggcattcg
     1741 gcactgcctg tatcagggcg agttgttgca gcagtatggc gagttcgccc aaggcgttgt
     1801 caaagccgac ggcacacgcc gccatttccc gcgctttggc ggtcagggct gcgccgtctt
     1861 ggttgatgat gcctgtcagc agttcgtaaa ggtattgttt gtcaaccgcg ctgatcattt
     1921 ggcggacatc gttttcggca actttgcccg aacctagggc gatggcttgg tcgagcaggc
     1981 tcaaggcatc gcgcatcgat ccggcggcgg cacgtcccaa aagttgcagg gcggggggtt
     2041 cgtaggcaat tttttcgctg tcgaggacgt gggcgaggtg gtcggcaacc tgctgcgaag
     2101 tcatattgcg caagacgaat tgcaggcagc ggctcaagac ggtaacggga actttgtgcg
     2161 gatcggtggt ggcgaggatg aatttgacgt gttcgggcgg ctcttccagc gttttgagca
     2221 tagcgttgaa cgcgcttttg gaaagcatat gcacttcgtc gatgatatag actttgtatt
     2281 tgccggcagt cggtgcatat tgggcgtttt ccaatacttc gcggatgttg tcgatgcctg
     2341 tgttggaggc ggcgtcgatt tccaataaat cgacatagcg tccggcatcg atctgcgtac
     2401 agctttcgca cacgccgcaa ggttcgccgt gttgcgcgtt ttcgcagttg aggcttttgg
     2461 caaggatgcg ggcgatggtg gttttgccta cgccgcgcgt tccggtcagc aggtaggcgt
     2521 ggtgcagcct gccttcgtcc aaggcgtttt gcagggcttt gacgacgtgt tcctgaccga
     2581 ctaagtcggc aaaggttttg ggccgccatt ttcgggcgag aacttgatag gccatatttt
     2641 tctcttggtt tcggtcgtga tgtttctgtc ggtgcgtcgg aatgccgtct gaacggcggt
     2701 ctcgggcggc gtattctagc actttcggct tattgtccgc gcaagaacag tgcgtccaat
     2761 tctttcaaag tcagtttgac ccaagtcggc ctgccgtggt tgcactggtt gctgcgcggc
     2821 gtgttttcca tatcgcgcag cagcgcgttc atttcgggca gggtaagccg gcggccggcg
     2881 cggatcgagc cgtggcagga catcgtggcg aggatgcggt tttcgtgttc ctcgatggtt
     2941 tggctgctgc cgacttgggc gaattcgttt aatacgtctt tggcgaggga gacgacatcg
     3001 gatttgccca acattgcagg cgcggcgcag gcggcgaggg tgttgccgcc catgtcggac
     3061 agttccagcc cgaagcctgc cagcgtttcg gcataatcgg caagggcggc gcattcttcg
     3121 tgggacgcgg caaaggttac gggaataagc aggcgttggc tttgcagctt gccgttttcc
     3181 tgacgttggc gtttcatttt ttcgtagttg acgcgttcgg cggcggcgtg catatcgatg
     3241 agcaacaggc tgtcttcggc ttgggcaagg atgtagatgc caagtaattg agcaatggca
     3301 aagccgagcg gcggcagttc ggatggggac gggatgccgt ctgaaagcgg catatctgtt
     3361 ttttgggcag gcgtttcaga cggcatattg ccgaaacgtg cctgctcgaa ttggctcaac
     3421 tcaaggtcga tgtcgtcggt ttttttgtag agctcggcgt aagtattcat tgccgcgcgg
     3481 ctttcgcgca gggacaggct gtgttgttgc ggcgcgcgcg cggcttggta aggcatgggc
     3541 gcggttttgc ctgacgaaac aaaggcattg cgtatatcgg gtttgttgcc tgtcggatag
     3601 ttggatgcgc gatcaaacag attttcgctg ttgttttcag acggcattgg tgcaggcgta
     3661 acgccagtaa tgtcatgcaa cacttcgcct gcgttgctga cgctttcggt caggtcggcg
     3721 cgtgtgtcgg caagggcttt attgagtgcg tggaacacga gttggtgcac ctgctggctg
     3781 tcgcggaagc ggatttctgt tttggtcggg tggacgttga catccacggc ttcgggcggc
     3841 aggtcgagaa agaggacgaa agcaggggta agggcgttgt gcaatacgtc tcggtatgcc
     3901 tgtttgacag catggagcat gactttgtcg cgcacgaagc ggtggttgac gaagcaatat
     3961 tgtttgtcgg ttttgccttt ggcgaaagtc ggcttggcaa tcgcgccgta gagccgcagt
     4021 gcgccgttgc cgctgtctat ttctaaggat gccgtctgaa agtcttcgcc gacaatggcg
     4081 gcaatccgtt catgcaggct ttgggctgga agtttgaaca cttgtttgcc gtcgcgtttg
     4141 agtgagaagg caatgtgcgg atgcgccagc gcgaggcgtt cgatcatggt ggcgcagtgg
     4201 gcgtattcgg tgttttcgga tttgaggaac ttgcgccgtg cgggagtgtt gaagaagagt
     4261 tcggcagctt cgatggtggt gccgatgggg tgggcggcgg cggtggggct gctgagtttg
     4321 ccgtcttcgg ctttgacttg ggttgcgtgc gaactgtctt cttgacggct ggtcagggtc
     4381 aggcggctga cggaggcgat gcttgccaag ccttcgccgc gaaagcccat gctggcgacg
     4441 tgttccaaat cgtttaaggt tttgattttg ctggtggcgt ggcggtggag cgcaagctcg
     4501 atgtcgtcgg ggtggatgcc gctgccgttg tcgctgacgc gaatcaggcg gatgccgccg
     4561 ccggccagct cgacatcgat cgccgtcgcg cctgcgtcta tgctgttttc aacgatttct
     4621 ttcagggcgt tggcagggcg ttcgaccact tcgccagcgg cgatttggtt gacaagatgg
     4681 tcgggcaggg cggcaatgcg tggcataagg taggcttccg ttgcaaaaaa cgcttcattc
     4741 tataataaaa ccccttactt ttctgcccgt attcttgata aaagccgttt atcccgtttg
     4801 gaaaatacca gtataatcac gccgtatccc ataaaattgg agcacaaaga tgtatcacta
     4861 ccaatctgat gccacacaat tcctcaaccg tctgattgaa gaaaaacctg agttggaaca
     4921 gcagcgtttg gaaaacaggg ggcttttgtg ggatgtcgaa ctcaatcccg aagaacagga
     4981 aaacttcgag gcggcaaaag tagcgaaaaa accttatacc tattaccaag actgaacccg
     5041 ccgaaatgac gaccaatctg tccaatgtcg caccggacct gcaaaactat ctgaacagca
     5101 tcggcgaacc cgaacatccc gttttgacgc ggctgcgtga gaagaccgag catcaccgta
     5161 tgggcaaaat ggcgattgcg cgcgaacagg cggcggtttt ggtttggctg gcaaagctga
     5221 ttcgcgcgga aaaatatctg gaaatcggcg tgtttaccgg atacagcagc accgcgcttg
     5281 cattggcgtt gcccgaacac ggacggatta ccgcctgcga catcaatgta acctttaccg
     5341 atacggcgcg tcaggtttgg aacgaggctg gtgtggcaca taaaatcagc ctgcacctgc
     5401 aacccgcatt gctgacattg gatgatttga ttgcacaggg tgaagccgga agctacgatt
     5461 tggcactgat agacgcagac aaaccgccca cgccgcaata tttcgagcgt tgcctcaaac
     5521 tcgtccggca aggcggcatc atcgctatcg acaatatttt gttgaacgga agggtgatgc
     5581 gcgaagccgc ttccgacgcg ccgcccagcg tcggcatcct caaagatttc aatcaaaacc
     5641 tgccgaacga tacgcgcatc gtccccatca ccctgcccgt cggcgacggc ttgaccctgc
     5701 ttctgaaaaa ataatgaaga ccaaattacc gctttttatc atttggctgt ctgtgtccgc
     5761 cgcctgttct tcccctgttt cccgcaatat tcaggatatg cggcccgaac cgcaggcaga
     5821 ggcaggtagt tcggacgcta ttccctatcc cgttcccact ctgcaagacc gtttggacta
     5881 tctggaaggc aaaatcgtcc ggctgtcgaa cgaagtggaa accttaaacg gcaaagtcaa
     5941 agcactggaa cacgcaaaaa cacacccttc cggcaggaca tacgtccaaa aactcgatga
     6001 ccgcaagttg aaagagcatt acctcaatac cgaaggcggc agcgcatccg cacataccgt
     6061 tgaaaccgca caaaacctct acaatcaggc gctcaaacac tataaaagcg gcaggttttc
     6121 tgccgctgcc gccctgttga aaggcgcgga cggaggcgac ggcggcagca tcgcgcaacg
     6181 cagtatgtac ctgttgctgc aaagcagggc gcgtatgggg aactgcgaat ccgtcatcga
     6241 aatcggaggg cgttacgcca accgtttcaa agacagccca accgcgcccg aagccatatt
     6301 caaaatcggc gaatgccaat acaggttgca gcagaaagac attgcaaggg cgacttggcg
     6361 cagcctgata caggcttacc cgggcagccc ggcggcaaaa cgcgccgcct cagccatacg
     6421 caaacgatag tttttccatt tttccgcttt ttaccccatc cgcaccaagg aggatttatg
     6481 attcaaatcg gcatcatcat gggcagcaac agcgattggc ccgttatgcg gcaggcagcg
     6541 cagtttcttg aagagttcgg cgtagaatat gaggcgcgcg tcgtttccgc acaccgcacc
     6601 ccggatttaa tgttccaata cgctgaaacc gcacgggagc gcggcatcaa agccattatc
     6661 gcgggcgcgg gcggcgcggc gcacctgccc ggcatggttg ccgccaagac caccgtcccc
     6721 gttttgggcg tacccgtccc cagcaaatac ctgcgcggcg aagattcgct tttatcgatt
     6781 gtacaaatgc ccaaaggtgt acccgtcgcc acattcgcca tcggcgaggc aggcgcggcc
     6841 aatgccgcat tgttcgccgt ttccatgctc gccaacgaaa accccgaact ggcgcaaaaa
     6901 ctggcagact tccgagccaa acaggaacag gctgttttga gtatggaatt ggaacagatt
     6961 taaaacccgc cgtccgcgaa tatatagtgg cttaaattta aaccagtacg gcgttccctc
     7021 gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
     7081 ccactataca aatgccgtct gaagccctgt ttcgggtttc agacggcatt tttgcgaaca
     7141 actttttatg cttgttcttt ttggcgcatt ttttctgcca tcagttcgtg cagggttttc
     7201 ttcgccggtt tcatcggcac gcggttttgc gtccaaccca actgtttgcg cggggtcagg
     7261 ttgcggaact tggtggctgc ccaaccgaag gcgcggtagg ttttgctgcc gctgaaaatg
     7321 ccgttgaatg tgcgccacgc catttgttcg ccgaaggtat gcgatgcgcc ttgccctcgg
     7381 atgggatgag gcacggtttc ggtcggcgaa cgttgcgctt cgacacgaag ccgctgcatt
     7441 tgttcggtaa tcgggatacg taccggacaa acttccacgc acgcgccgca catcgtgcag
     7501 gcggtcggca ggtcgcgagt ggcatccaga cccaacaggt gcggggaaat aatctcgcca
     7561 atcgggccgg gataggttgt gccgtatgcc gcgccgccga tgcgggtata aaccgggcaa
     7621 tggttcatac acgcgccgca acggatacat tgcagggtgc ggcgcatttg gtcttcggca
     7681 taagcctggc tgcggccgtt gtcgagcaga accaagtgca tttcttgcgg accgtctaat
     7741 tcttcactgc ggcgcgggcc ggtaatcata ttgaaataag tggtaatgtt ctgaccaatg
     7801 gcggagcgcg gcagcagact gtacaagggt gggatgtcgg acaatttcgc cactactttt
     7861 tcaataccgg taatggcgat atgcacgggc ggaacggtgg tactcaagcg accgttgcct
     7921 tcgttttcga ccagacacag cgtgcccgtt tcggcaacgg cgaagtttac gccgctcaag
     7981 ccgacatcgg cagtgctgta aatatcgcgc agggctttgc gggcgaagcc ggtcagttgg
     8041 tccacgtcgt ctgtaagcgg tgtgccgagg ttttggtgga acagttcgct aacctgttct
     8101 ttggttttat ggattgcggg catcacgata tgggtcggtt tttcacctgc catttggacg
     8161 atgaactcgc ccaagtcgct ttccaccgct ttaatgcctt ttgcttcaag ataatggttc
     8221 agctcgattt cctcgctgac catcgatttg cctttgacca tcagcttgcc gtttttggct
     8281 gtgatgatgt cgtggataat ttggcaggct tcggcagggg tttccgccca gtgtactttc
     8341 acgcccaact tggtcaggtt ttcttccaac tgctccaaca gcgcgggcaa tttggacagg
     8401 gaacgctggc ggatgtgttc gcacaaatcg cgcaggcttt gcagctcttc ttcgtcggtc
     8461 aaaacggctt tgcgtttggt catcagcata tccatcgcgg tacgcaggct tttgcgcaaa
     8521 ggcttgtctt gaagggaaat tgcggcgttt tgcttgaaag tttccggctt catgtggaac
     8581 ttgatggttt gcgtagtcat gcgttttcct ccaaatcggc aggggaaatg tggtcgggca
     8641 ggatggcgag gatgaccaaa tcgcgcgggc cgtgcgcgcc gtaagcaagc gtcagttgga
     8701 tgtctgcggt tttggacggg ccggaaatca ggaatacatt ggtcggcata ccgttttcta
     8761 ccagtttttc tccttcgacg gcattatgaa actcgttgta catcttggac gtatcaaaca
     8821 ggcagaaatg cacgggcgga acgaggctca aagtacgcgg ttcttcgggg ctggaaaaca
     8881 gcatcagtgt accggtacgg gcgatgccgc attgcgcgcc gctgaagccc gcatcgatgt
     8941 tcgtgaaaaa ctcggttttc caagtatcga tttcgcgctc gaagacaatc ggttcgatat
     9001 tgctgcccgc caatgcggca cgggcaattt gtccgtgttc ggtcgccaag ggcagcagga
     9061 tgtttttcaa acccttgcct tctgccgctt cgcggaaaac ctgcatccaa ttgcttttcg
     9121 tcacccaata aatttcggtt ttgaccgcgc gcattgcggc agcccaatgt ttcagacgct
     9181 cggcttcgct gtcccaagaa acgccctttt cgcggtaata atcgaaaacc gcaggctctt
     9241 ccatcggcaa ggcatcggct tttttcagtt ttgccaaaat attttcgcgc gcgctcatgc
     9301 tttgcctccg gtgcgttcca acaagaagga tgcgatatgt ttcggacgcg gcatatccgg
     9361 ctcgtccttg gcgattttgc cgccgatgtt catcatacag ccgcagtccg cgctgatgat
     9421 ttcggttgcg ccggtttctt tcagcgcggc gactttgtct gttaccattg cgccggaaat
     9481 atcggcttgt ttgacggaga atgtgccgcc gaagccgcaa cattcgcttt cgtggtcgtg
     9541 gacgatgcgt tcgacgtttt ccataccgtc aatcagttgc cagcccgaga gatggacatt
     9601 catttcgcgg cgggcggcgc aggaagtgtg aacggcgact ttgagcggtt cgcccttgtc
     9661 ttcgggtttg aaaccgatgg caagcaggaa atgggtaaac tcgatgatgc ggccggcgca
     9721 atccacagcc ctttcctcgt actcgctgcc tttaaacagc gtcggccaat ggtgtttcat
     9781 cataccgccg cacgaaccgg acggtacgac aatcggccac tcctcgggga aaaggtcgag
     9841 ttgcgctctg gcgacatcaa aggcttcggt cggatggccg gatgaatagg cgggctgacc
     9901 gcagcagctt tgcgccatcg ggaaatggac gcgtatgccc tgttgctcga ttagggtaat
     9961 ggcatccatg cctgcttcgg gcataaaaag atcaaggacg caagtgccga aaaaatagac
    10021 atcggtcgga ttgctgtcgt atcggatgat ttttggggga atggttgcgc tcatgttttt
    10081 tgataacggg aaacccgttt ttttctgtag aaaggtaagc gtttacttta agtaattgac
    10141 tgttgcgggt caagtctaat tttaaaaaat aatccggttt ttcttacaaa ctgccccata
    10201 acgcttactg taccttaatc tgatagtttt cgataataat tatcattaca atgcaatgcc
    10261 ggttcgtttg cttgtgaaca ttcaagatgc cgactctgac ggcattcaga cagcatctga
    10321 aaacaataac ggcacaagga tggggtacaa tcgctctatg gaaaaaaaca ccctatccgc
    10381 ccgcgcacca tccccttggc tgccgcttct gctggcaatt gccattttta tgcagatgtt
    10441 ggatgcgacc attttaaata ccgcactgcc tgaaattgcc gccgacctga atgagtcgcc
    10501 attgaacatg cagctggcgg ttatttccta cacgctgact gttgccctgc tgattccttt
    10561 gagcggttat ttggcggaca ggttcggaac gaaaaaagtc tttttcggtt cgattgccgt
    10621 ttttatgctc ggatcggcat tgtgcgccgc atcgggttcg ctgtttgaat tgacactttc
    10681 ccgtgtcgtt cagggcatcg gcggttcgat gctggttccg ataccgcgtc tgaccatctt
    10741 gcgtatgtac gacaagtcca agctgctcaa tgccatcaat tatgcggtta tgcccgcatt
    10801 aatcgggccg gttttagggc ctttggcggg cggttatttg gtcgaatacg cttcgtggca
    10861 ctggattttc ctgctcaacc tgcccatcgg tctgctgggt ttcatattgg gacgcaacat
    10921 catgcccgat attaaaggca gtaatatctc tttagacttc aaaggttatc tgattttttc
    10981 tgccgccgcg tgcctcttgt tactttcggc agaaagcctg tcgcacgcgc tgcctccgta
    11041 ttttgcactg tcgcacgcgc tgcctccgta ttttgcactg ttgccgctgt gcggcggact
    11101 gctgtttgca cgccgttatt tccgacatat gaaaaccgcg tccaaaccga tttattccgc
    11161 cgacctgttt ctgatacgca ctttccgtct gggactggcg ggcaatctgt tcagccgtct
    11221 cggcatcagc tcgattcctt ttctgatgcc cctgatgttt caaatcgctt tcggcttcgg
    11281 cgcaagcctg tcgggttggc tggtcgcacc cgtcgccctg tcttcgctgc tggtcaaacc
    11341 gctgattgca ccgctcatga aacgtttcgg ctaccgtacg gtactgcttt ggaacaccaa
    11401 gctgcttgcc gccttcatca tgctgctcgc cctgcctgac ggaaactcgc cgctgtggat
    11461 ttgggttttc ctctcgctgg cgatcggcgc gtgcaactcc ctacagtttt ctgccatgaa
    11521 cacactgacc ctcgccgatt tgcgcccgca acagaccggc agcggcaaca gcctgatggc
    11581 ggtcaaccaa cagcttgcca tcagcatggg cattgttgtc ggcgcattaa tccttaaaaa
    11641 ctggacattt ctgataccgg cttcttcagg tctgcattcc gccttccgta tgaccctgct
    11701 cagtatcggc ggcattaccc ttgcctcatc gctggttttc aagcgtctgc acgtttcaga
    11761 cggtgcaaac ctgacacgga acacaccgtc ctaaagcggt ccgcacgcaa aacttttacc
    11821 cgtttcaacg tttggattat gattccgcac ttccatgcgc gccaacccca aaacacaggc
    11881 aatgccgtct gaaaccatat ccctgatgaa aacacgcagc ctaatttccc ttttatgcct
    11941 ccttctctgt tcatgttctt catggttgcc cccactggaa gaacggacgg aaagccgtca
    12001 tttcaatact tccaaacccg tccgcctgga caacatcctg caaatccggc acacccctca
    12061 taccaacggg ctatccgata tctatctgtt gaacgacccc cacgaagcct ttgccgcccg
    12121 cgccgccctt atcgaatctg ccgaacacag cctcgatttg caatactaca tctggcgcaa
    12181 cgacatttcc ggccgactgc tgttcaacct cgtgtacctt gccgcagaac gcggtgtgcg
    12241 cgtacgcctg ctgttggacg acaacaacac gcgcggattg gacgacctcc tgctcgccct
    12301 cgacagccat cccaatatcg aagtgcgcct gttcaacccc ttcgtcttac gaaaatggcg
    12361 cgcactcggc tacctgaccg acttcccccg cctcaaccgc cgcatgcaca acaaatcctt
    12421 taccgccgac aaccgcgcca ccatactcgg cggacgcaat atcggcgacg aatacttcaa
    12481 agtcggtgag gacaccgttt tcgccgacct ggacatcctc gccaccggca gcgtcgttag
    12541 cgaagtatcg cacgactttg accgctactg ggcaagccat tccgcccaca acgccacgcg
    12601 catcatccgc agcggcaaca tcggcaaggg tcttcaagca ctcggataca acgacgaaac
    12661 gtccagacac gcgctcctgc gctaccgcga aaccgtcgaa cagtcgcccc tctaccaaaa
    12721 aatacaggca ggacgcatcg actggcagag cgtccaaacc cgcctcatca gcgacgaccc
    12781 tgcaaaagga ctcgaccgcg accgccgcaa accgccgatt gccgggcggc tgcaagacgc
    12841 gctcaaacag cccgaaaaaa gcgtctatct ggtttcaccc tatttcgtcc ccacaaaatc
    12901 cggcacagac gcactggcaa aactggtgca ggacggcata gacgttaccg ttctgaccaa
    12961 ctcgctacag gcgaccgacg ttgccgccgt ccattctggc tatgtcaaat accgaaaacc
    13021 gttgctcaaa gccggcatca aactctacga gctgcaaccc aaccatgccg tccccgccac
    13081 aaaagacaaa ggcctgaccg gcagctccgt aaccagcctg cacgccaaaa ccttcattgt
    13141 ggacggcaaa cgcatcttca tcggctcatt caacctcgac ccccgttccg cacggctcaa
    13201 taccgaaatg ggtgtcgtca tcgaaagccc caaaatcgca gaacagatgg agcgcaccct
    13261 tgccgatacc acacccgaat acgcctaccg cgttaccctc gacaggcaca accgcctgca
    13321 atggcacgat cccgccaccc gaaaaaccta cccgaacgaa cccgaagcca aactttggaa
    13381 acgcatcgcc gcaaaaatcc tatccctgct gcccatagaa ggtttattat agaaatatag
    13441 cggattaaca aaaaccagta cgacgttgcc tcgccttagc tcaaagaaaa cgattctcta
    13501 aggtgctgaa gcaccaagtg aatcggttcc gtactattcg tactgtctgc ggcttcgtcg
    13561 ccttgtcctg atttttgtta atccactata ccgtctgaaa caccttcaga cggcatatcc
    13621 gaacccgcaa aggaaaaacc atgtttcccc ccgacaaaac ccttttcctc tgtctcagcg
    13681 cactgctcct cgcctcatgc ggcacgacct ccggcaaaca ccgccaaccg aaacccaaac
    13741 agacagtccg gcaaatccaa gccgtccgca tcagccacat cgaccgcaca caaggctcgc
    13801 aggaactcat gctccacagc ctcggactca tcggcacgcc ctacaaatgg ggcggcagca
    13861 gcaccgcaac cggcttcgat tgcagcggca tgattcaatt cgtttacaaa aacgccctca
    13921 acgtcaagct gccgcgcacc gcccgcgaca tggcgacggc aagccgcaaa atccccgaca
    13981 gccgccttaa ggccggcgac ctcgtattct tcaacaccgg cggcgcacac cgctactcac
    14041 acgtcggact ctatatcggt aacggcgaat tcatccatgc ccccagcagc ggcaaaacca
    14101 tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa ctacctcggc gcacatactt
    14161 tctttacaga atgagccgcc cgccgcgccg gaatcgcggc aaaatgccgc cttattccgc
    14221 tgtttcggtc agcgatgaga acacttcaaa ataagtcggg aaggttttgt gggtgcattt
    14281 cgggtcgttg atgacgacgg gtacgcccaa caaggaaacc agcgagaaac acatcgccat
    14341 acggtggtcg tcgtaagtgt cgatgacggc gtcgggcgtc agcgtttcag gcggggtgat
    14401 gtgaatcgct tcggcttctt cggcgacttt tgcaccgagt ttgcgcaact cgtttgccat
    14461 tgcggcgatg cggtcggtct ctttgacgcg ccatgaaccg atattgcgca gcgtacaggt
    14521 ttgccctgtg gcaagcgcga cgatggcgag ggtcatggcg gcatcgggga tatggtttgc
    14581 atccaaatca aaggcttgga cggcacgttc tttcgggcgc gagacttcga cgaagttttc
    14641 gccccaaacc acgtccgccc cgattttttc cagttcgtgg gcaaaggcga catcgccctg
    14701 tatgctgttt gcgccgatgc cggtcacgcg gacgggcgtg gcggcaatca aaccggctgc
    14761 gaggaagtag gacgcgctag aggcatcgcc ttcgacgtgc aagtgttcgg gcgcgtggta
    14821 gtgcgcatcg gcgggaattt tgaagacgcg gtagccttca ttgataacct gtacgccgaa
    14881 ttgcgccatc agttttaaag taatgtcgat atagggcttg gaaattaact cgccgaccat
    14941 acggatttca aacgcctgcc tggtcagcgg taacgccatt aaaagggcgg tcagaaactg
    15001 gctggacaca ttgcctttaa tcggaatcac gcgctcgccg ttgtcttggc gttcgccgat
    15061 atgaagcggc ggatagtgtt ccttgccgag atattcgaca tcggtcccgg caatccgcag
    15121 tgcatcggtc aaatcgccga tggggcgttc gtgcatacgc gcaacgccgt gcaaatgata
    15181 atcgccgccc aaaacggcaa gcgcggcggt cagcgggcgg aacgccgtgc ccgcgttgcc
    15241 caaaaacaaa tcggcagtgc ggttggggaa gcgtccgccc gtgccgtgca ctttcagacg
    15301 gtcttcggca agatattcga tttcaacgcc gagtttatcg agtgcttcga gcatacggtc
    15361 ggtatcgtcg gatttgagca gggaatggat ttcgcaagca ttgtcggaca aggcggcaag
    15421 cagcagggtg cggttgctga tgcttttgga gccgggcagg gcgacggtgg aaggtttgag
    15481 acgggcgacg gggaggcgga cggattcggt catggcaaaa cgtcaatatt ataaagataa
    15541 aaacagcctg cattatactg gtgcaaatgc cgtatgaaaa atctcaggtt tggcattttc
    15601 ggttttaaag tccgtaaatg tggtttttta tgccgaaaat tgattatttt taaatttttt
    15661 gtttctaaaa tttctttgtc ggcatatttt cagcttttgt tgcggatatg gcgcgtttgc
    15721 cggcggcgcg gcacgattgc caatttcata gaatttggta gaatagccgc tgttcaacga
    15781 cagacaagcc gccgattttc cgggcggctt gtatttttat tttaatatcc ggacgcacaa
    15841 aacaagacgg tacgcggtat gtttgctgcc gcaaaccggt gtgccggaat atggagaaaa
    15901 aagaccgatg caaaaaatcc ccctgaccgt acgcggtgcg gaattgctga aacaggaatt
    15961 gcagcagctc aaaagcgtgg cgcgtcccga agtgatcgaa gcgattgccg aagcccgttc
    16021 gcacggcgat ttgtccgaaa acgccgaata cgaagccgcc aaagaacgcc aaggttttat
    16081 cgagggccgc atttccgagt tggaacataa actttccgtt gcccatatta tcaatccgac
    16141 cgaaatccac gccgaaggca aaatcgtgtt cggtacgacg gttacgctgg aagatttgga
    16201 aacggaagaa cacgttacct atcaaattgt cggcgaagac gaagcggata tcaaacaggg
    16261 caaaatctat gtcggctcac cgattgcccg cgccttaatc ggcaaggaag agggggatac
    16321 ggcggaagtc caagccccgg gcggcgtgcg cgaatacgac attatcgaag tccgatatat
    16381 ttgattcggc ttgatttcga tacacccgac acacgcagga aattatagtg gattaacaaa
    16441 aatcaggaca aggcgacgaa gccgcagaca gtacaaatag cacggaaccg attcacttgg
    16501 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtaccggt
    16561 ttttgttaat ccactataac agcaaccctg tcgccgtcat tcccgcaaaa gcgggaatct
    16621 aggacgcagg gttaagaaaa cctgcatccc gtcattccct caaaaacaga aaaccaaaat
    16681 cagaaaccta aaatcccgtc attcccgcgt a
//
LOCUS       DAXIEB010000039        16401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000039 DAXIEB010000000
VERSION     DAXIEB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.039"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..545)
                     /locus_tag="ACFOA3_001008"
     CDS             complement(<1..545)
                     /locus_tag="ACFOA3_001008"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6898033.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEI"
     gene            complement(614..1438)
                     /locus_tag="ACFOA3_001009"
     CDS             complement(614..1438)
                     /locus_tag="ACFOA3_001009"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6898034.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            1529..2161
                     /locus_tag="ACFOA3_001010"
     CDS             1529..2161
                     /locus_tag="ACFOA3_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6898035.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(2367..4151)
                     /gene="rmuC"
                     /locus_tag="ACFOA3_001011"
     CDS             complement(2367..4151)
                     /gene="rmuC"
                     /locus_tag="ACFOA3_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6898036.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAVSVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(4490..5278)
                     /locus_tag="ACFOA3_001012"
     CDS             complement(4490..5278)
                     /locus_tag="ACFOA3_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6898037.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(5293..6642)
                     /locus_tag="ACFOA3_001013"
     CDS             complement(5293..6642)
                     /locus_tag="ACFOA3_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6898038.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(6661..7242)
                     /gene="petA"
                     /locus_tag="ACFOA3_001014"
     CDS             complement(6661..7242)
                     /gene="petA"
                     /locus_tag="ACFOA3_001014"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6898039.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(7365..8114)
                     /locus_tag="ACFOA3_001015"
     CDS             complement(7365..8114)
                     /locus_tag="ACFOA3_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6898040.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVGQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            8245..9174
                     /locus_tag="ACFOA3_001016"
     CDS             8245..9174
                     /locus_tag="ACFOA3_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6898041.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAKDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(9430..9822)
                     /gene="rpsI"
                     /locus_tag="ACFOA3_001017"
     CDS             complement(9430..9822)
                     /gene="rpsI"
                     /locus_tag="ACFOA3_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6898042.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(9832..10263)
                     /gene="rplM"
                     /locus_tag="ACFOA3_001018"
     CDS             complement(9832..10263)
                     /gene="rplM"
                     /locus_tag="ACFOA3_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6898043.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(10441..10620)
                     /gene="ssrS"
                     /locus_tag="ACFOA3_001019"
     ncRNA           complement(10441..10620)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFOA3_001019"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(10626..10931)
                     /locus_tag="ACFOA3_001020"
     CDS             complement(10626..10931)
                     /locus_tag="ACFOA3_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6898044.1"
                     /translation="MNIEQVHIEVMNARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(10942..11271)
                     /locus_tag="ACFOA3_001021"
     CDS             complement(10942..11271)
                     /locus_tag="ACFOA3_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898045.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(11326..12315)
                     /locus_tag="ACFOA3_001022"
     CDS             complement(11326..12315)
                     /locus_tag="ACFOA3_001022"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6898046.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(12438..15140)
                     /gene="ppc"
                     /locus_tag="ACFOA3_001023"
     CDS             complement(12438..15140)
                     /gene="ppc"
                     /locus_tag="ACFOA3_001023"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6898047.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            15183..16076
                     /locus_tag="ACFOA3_001024"
     CDS             15183..16076
                     /locus_tag="ACFOA3_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6898048.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKLKTEALADRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
BASE COUNT         3601 a         4471 c         4279 g         4050 t
ORIGIN      
        1 taaatctcgc ccagattgcg ccagcagccc gccgcatcca tgccgtaacc gaaaacataa
       61 cggttcggca catccagtcc gacataatcg gctcggatag gcttgggttt gtcaatcagc
      121 ttgttggcga acaccgccgc acggcagctt gccgcaccca tttccaaaag tttggcttga
      181 atggcggaca tcgtatgccc ttcgtccaaa atatcgtcca gcacgacgac gtgcctgccc
      241 cggatttgtt ccgcatcggg catacgcttc cagttgaacg cgccgccctc cagcttgtcg
      301 ccgtaacggg aaacgtgaac ataatcaaaa tctaagggaa aacgcaacag cggcagcaac
      361 tgccccgtaa acaccaccgc gccgcccatc acgggcagca gcagcggata tttgccgccc
      421 aaatcgcgcg taatctcgtc cgccactttt tgcagtgcgg cacggcattg gccttggtcg
      481 aacaaaagat cagcgttttc aagcatcgcc tgtgtttcaa ggcgtttggt ttctaaatcg
      541 gtcatatgtc ggaatcggtc ggtaaaagga aaattataaa ccaaagtatc ggatgccgtc
      601 tgaactgtcc tgctcagcgg tcggtacgca cgcgcacaaa tgtggcaaat ttcggcgtgc
      661 ctttccgcgt aaagccacgg taacggtagg taatcagcgt gccgattttg ggcgggttgt
      721 cgcggtcttt gtccttgaaa ccgctgccga tgcggaattc gccgtgtcgg tttttgcagc
      781 cgaccgcgcc cagccgtccg gcgtttcgcc ctttgccctc atagtgccgc gttaccgtgc
      841 attcgtcgtc gtattggctt ttcagcttca ataattggct gctcctgccg ccgctataac
      901 gggattcggg ctggcgcagc atcacgcctt cgccgccctg cgcttcgatt tgttttaaaa
      961 agtccatcgc gtgctgccgg tcgcgcactt tgatttgcgg gatgatggta atcggcgcgt
     1021 tcggatgcgt tttcagccac tgcgttgcga ctgccaaacg ttggtagaga ttgccctgcg
     1081 ccttgggtac gtcgaaaacg tgcagacgga tgccgcgcca gtctgaagaa acagaacgca
     1141 cggtagcgga aatctgctcg aactgaccgc gtccgctata caattcgccg tccaaaggat
     1201 aaggcggaaa ctgcgcggta aagcctttgg ggggcgtaaa cgcgtagccc tgacggctta
     1261 tcaggtgctt tccgtcccaa taggcgcgca cgccgtcgag tttctcgctc attgcccagc
     1321 ctgcaatctc ttggtctttg tattcctgcg ccagcatcaa atccgccgcg cctgctgatg
     1381 cagggatgaa aaccgccgta aaaatcggta tgatgccgcc gattgtcttc ttaatcatct
     1441 gattccccca atatcaaaac aggcggcaaa ccgccataaa acaaacggca aacccgatgc
     1501 cgtctgaaaa accgtttagg aacacgccat gaccctacgt tacgaaatcc tctccgttac
     1561 ccccttccgc caaaactgca ccctgatttg ggacgacgaa agcggcgaag ccgtcctgac
     1621 cgatgtcggc ggcgacgtgc cgttcctgct gcaagcgttg gcaaaccgca aacttacgct
     1681 cacggcaatc tggctgacgc acggccatct cgatcacgcg ggcggcgtgg tcgaaatgtt
     1741 gaaaacgcat aaagtccctg tcctcgggcc gcatccggac gatgaattcc tgctccaatc
     1801 gctgccgcaa accaccgcgc aatacggatt tcccgtctcg cccgcctttg cgccgaaccg
     1861 ttggctcgaa gaaggcgaaa cgctcacggt cggacgctat gcctttcaag tgctgcatat
     1921 tccgggccat acgccgggac atatcgtctt ttattgtgcc gaggcggaat tgctgattgc
     1981 gggcgacgtg ctgttttacg aaaccatagg cagaaccgat tttccgcgcg gcaaccacgc
     2041 cgacttaatc aataatatcc gcaacaaatt attcaccctt cccgaaaccg tgcaagttgt
     2101 cgccggacac gggcgtatga cttccatcgg acacgaaaaa cggcacaatc cgtttttcta
     2161 accgacttca ctacggtctt cagacggcgt catctgcact gatgccgtct gaaacgcaaa
     2221 aggctcaggc aaccgccgcc ttgccggtgt acctcgccgg acaaggcttt ggtaagtact
     2281 tcaaaccaac cccaaatcag aaccggtaca gttacgccgc gctttcggca ttcccgcccc
     2341 tgctgaaaca agcttttccg cacaagtcag actgcttcat cttctgccac atattccaaa
     2401 gattccgaca acgccgttgt ttcattggca cgttcggtca aatcgcgtgc aagctgcttg
     2461 tttgccttca cacccaacag acgcagtttc tcggcgcggc cgaccagatt cccgcgcccc
     2521 tcggcaagtt gcttaaatgc cgtctgaaag ctgttttgcg cctgatcgat gcctttgccg
     2581 acgctttcga gcgtctgtac aaagccgaca aatttgtcgt acagcttgcc gccttcgtcc
     2641 gcaatcgcca gcgcgttttg gttttgctgc tcgttgcgcc aaatattcgc caccgtccgc
     2701 aaagtcgcca gcagcgtact ggggccgacc agcataatcc gtttgtcgaa acactcttgg
     2761 aacaagccag cgtcattctg caacgccaac aggtaggccg gttcgacagg gataaacata
     2821 aagacgaaat ccaatgtgtt cacaccttcc aaatcggtgt aatccttcag cgacaagcct
     2881 ttcatgtgtg cacggatgct ggcaacgtgt gccgccagtt cgcgtgccgc cgtatccgca
     2941 tccgccgcct gcgtgtagcg cacataagct gtcagcgaga ccttggagtc aatcacaatc
     3001 tgcttgttgt cgggcaggtt gaccaaaacg tcgggctgga ggcggcgcgt gccgccgtct
     3061 tcctcttttc ggacggatac cgcctgaacc acatattcgc gccctttctg gaggccggaa
     3121 ttttccaaaa ccgtttccaa aatcatttcg ccccaattgc cctgaacctt gttctgcgta
     3181 ccggtcagcg cgttggtcag tgcctttgcc tcgctgtgca gctgcgcatt caatccttga
     3241 agccgtttca attcgttttc caacgtcagc cgctcgcgcg attctttatc ataggtttgc
     3301 ttgaccaact cgccgaaacc gtggatgcgt tcgttcaacg ggttcaaaac ctgatggagc
     3361 tgctcgcggt tctgctcggt aaaacggcgg cttttttctt ccaaaatcgt gttggcaaga
     3421 ttttgaaact gatcgctcaa acttttgcgc gcctcgccca gcaaggacag cttctcttca
     3481 gaagcaaggc gttcctgttc gatttgcgtt gccaaacgtt cgttttcaac cgccaaaccc
     3541 tgtgcctttt cctgcaactc ggtatgcgac tgcctcaacc gctccgcttc cgcctctttt
     3601 tcctgcaaat gggcaatctg tttttcggct gcggcaaaac ggttgcccac atcggaaagg
     3661 tcgttttgca cgtcgcggac agtttggcgg ctttcttcca aatcggtttc gattctttgg
     3721 cggatttggc gttccaaggc atattggtcg gcaaacgcct tccgttcctg tccgagctgc
     3781 gttgccaaac gttcgttttc aaccgccaaa ccctgtgcct tttcctgcaa ctcgatatac
     3841 gactgcttca gccgcgccga ctccgcctct ttttcctgca aatgggcaat ctgcttttcg
     3901 gctgcggaaa aacggttgcc caaagcataa ttttcgtcct gcaaatgccg atatttcccg
     3961 tccaacaccg ccaattccga cacggttttg ccgtgcgcct gttcgacaaa atcacatctt
     4021 gccgcctttt ccgccagttg cgcgttcaaa tcggcaaact cgccctgaaa cctgcccttc
     4081 atcagcaacc atgtaaacaa cacgcccgac accaacgccg ccaaaggcag caaaacagtc
     4141 atcagttcca tcaatcatcc taatattcaa acattttcac accgggcaaa accgccgctt
     4201 attccgattc tacctgtttg ttcgacatag ctccaaaaaa tatagcggat tggctttaaa
     4261 cctgttcgac atcgccttac catgctgctt gcggtttcag accttttcct aattcaatat
     4321 ccatccgccg caaaccctga ttaagttccc gatgtctgac atttttagaa tgatgccgtc
     4381 tgaaatgttg cagctatgtt cagacggcat acggattcag gcttttcaaa cggcaggcaa
     4441 aatgaaaaaa gggcaaaccc taaaggattt gcccttttgt tccaaacgct tagtgtacgt
     4501 ctttccaata ttcttttttc aggaaatacg ccaaaggcag cataaccgca aataggaaaa
     4561 tcatcacgac atagcctata cgtttgcgtt gcagttgtgc aggttcgccc atgtacacaa
     4621 ggtaattgac caaatcgcgt acatatgcgt cgtactcttt ctggatcact ttgccgttag
     4681 gcagacggcg gctgtgcaaa ccggtagatt cccaatacag cttaggcttc atctcgccgt
     4741 gttcgtcttt taccataacc ggctgacctt tggcatccaa ctcaacggct tgaacgcctt
     4801 gctgttccca caacgggtgg ggcataccga ctttatcgaa tacagtattg ttccagccgc
     4861 tcggacgggt cggatcttta tagaagccgc gcatataggc gtaaaggtag tctgcacctt
     4921 tggaacgcgc aatcaacgtc aaatcgggcg gagcagcacc aaaccatttt gccgcatctt
     4981 tcgggttcat cgccgaatgc atgacatcgc cgacattatc ggtggtaaac atcaggtttt
     5041 tcttgatttc ttcgtcagtc aaaccgatgt ctttcaaacg gttgaaacgc ataccgcttg
     5101 ccgagtggca ggacaggcaa tagttggtaa agatttgtgc gccgcgctgc aggctgactt
     5161 ggtcgcgcaa atcgatatcg actttctcat agtgtgcatg accgcccgaa gcaaatgccg
     5221 cacccatggg tactgccaac agcaaggcag caaaccaatt tttcatagct tgtttcattt
     5281 tgactgccct catcagatgt tggttgcaaa caggtaagca cccacggcgg taatgccgac
     5341 gtaaacaaag aacatgattt tttgtttggc ggtgctcatg gttacgcgtt cgggaaccgg
     5401 tttgttttta tccagcttgg tatagaacgg catacccagg aagaatgcga aatagacgaa
     5461 agacaggatg cgcgcgacca aagtacgcgt atcggttgcc accatcgcgc ccaaaatacc
     5521 caaaccgata aaggaaatga tgaacaagac caatgcggtt ttgaaaatcg gaccgcggta
     5581 acggacggat ttcacctcgc ccctatccag ccaaggcagc agggcaatca acacgaccgc
     5641 cgcgcccata ccgattacac cccaaacctg cgttcccaag aacgagggaa tcgcacgcaa
     5701 aatggcgtag aacggtgtga aataccatac cggcgcaatg tgcggaggtg ttttcagcgc
     5761 atttgccgca tcgaagttcg gcgcttcgag gaagtagccg ccgccttccg gtgcgaagaa
     5821 caatacggca cagaagacaa tcaggaatac aaccacaccc aagatgtctt taacggtata
     5881 gtaaggatgg aatgggatac cgtcgcgcgg gataccgttt tcgtctttgt ttttcttgat
     5941 ttcaacgccg tcagggttgt tagaacccac ttcgtgcagg gcgatgatgt gcgccacaac
     6001 caagccgagc aataccagag gtaccgcgat aacgtgcagg gcaaagaaac ggttcagggt
     6061 aacgtcggaa acgttgaagt cgccgcggat ccaagtggac aaatccggac cgataacagg
     6121 gatggcggag aacaggttaa taattacctg cgcaccccaa aaggacatct gaccccaagg
     6181 cagcaggtag cccataaagg cttccgccat caatgccaag aaaatcaggg agccgaaaat
     6241 ccacaccaat tcacggggtt ttttgtacga accgtaaatc aaaccacgga acatgtgcag
     6301 ataaacaaca atgaagaaga aagatgcgcc ggtggaatgc atgtagcgga taatccaacc
     6361 gccggacacg tcgcgcatga tgtactcgac tgcggtaaag gcagcaggca gatggtaggc
     6421 gttaaggttg ccgtccggtt tgtagttcat ggtcaggaaa atgccgctga cgatttgaat
     6481 cacgaggaca agcagggcca aagagccgaa gtagtaccaa aaattaaagt ttttcggtgc
     6541 gtagtattga gccaaatgct cattccacat tttagacagg gggaaacgag cgtccatcca
     6601 gcctaacaat gcttttgctt tgctattggt ttggtttgcc ataattatcg ttccttattc
     6661 ttagtcttcg cccaccaaga tagttgtgtc gctcaagtat ttatatggcg ggacaaccag
     6721 gttggtcggg gcaggaacac ctttatatac gcggccggcc aagtcgaatt tcgaaccgtg
     6781 gcaggggcag aagaagccgc ctttccagtc tgcacccaaa tcggcggggg caatgtcggg
     6841 acggaaggtg ggcgagcagc ccaaatgggt gcagataccg atggcgacaa ggatgttcgg
     6901 cttaatcgaa cgggtctcgt ttttagcata ctccggctgc tgttccgcat cggaattggg
     6961 atcggtaagt tcgccgttca ggcctttcag gtctttaagc tgctgatctg tacggttgag
     7021 cacccaaatc ggtttgcctt gccactcggc ggtcagcagc tgacccgctt cgattttact
     7081 gacatccacc tcgacggcag caccggcggc cttggctttt tccgaaggga aaaaactggc
     7141 cacaaacggc gttgccacac ccaatgctgc cactccgccc gcgccgcagg tcgcgagtgt
     7201 caggaaacgg cggcggccat tgttgatttc ttgattatcc attattcagt cgtcctaata
     7261 ttttgggaat accgagccat taaacgttgc aattttaccc agtttgcagt gatactcaaa
     7321 gcattattta aaataaggta aagttttatg atatttctca agactcaagc cggattgttt
     7381 tcgtcaaaat ggcacacttc caacccgaaa acctctgccg ccgattctgc cagcgcgcgt
     7441 acgccgtaac gttccgtcgc gtgatgccct gccgaaatga aagccgtacc cgtttcattg
     7501 gcaaggtggt attgggcttc ggaaatttcc cccgtcaaat acagatcgac accttcgtct
     7561 attgccgtct gaaaaaaccc ctgcgccccg ccgctgcacc atgcaacccg tcggatttcg
     7621 cgttcgggtt tgccgatgac gacaggctta cgttgcaaaa ctgtttcaat atgcgccgcc
     7681 aatgcgccga gtgtcttggc ttgtttcagg ctgcccgagt tgagcaggtt ttgttcgccg
     7741 aaccgttttt ctgtcgcaaa acccaatctg tcggcgagtt gggcattgtt gcccagtgtg
     7801 ggatgtgcat ccaggggcag atggtagcct gccatattga tgtcgtgccg taacagtgcg
     7861 gcaatccgtt cttttttcca accagtaacg gtcggcaact cgtttttcca gaacataccg
     7921 tgatgtacca aaagcaaatc tgccttctgc cccacagcaa aatcaatcgc tgccctgctt
     7981 gccgttaccg acgtaacgat tttcccgata tattccctcc cttcaacctg caaaccgtta
     8041 ggggcgtaat ctttaaacaa cgctgtctgc aatgtttcat tacaccaagt cagaaaatcc
     8101 ctgcgcaata ccatcttttt tcctaatcgc tttaaacaag cgggcattct aatcgcaaaa
     8161 tgtctggaat tcacattttt ccgatttgca cccgcatatg aattatttta atatgcgccg
     8221 gttcaatatg ccgtctgaaa cctcatggat tccatcatcg aactgcgcca cctcaagacc
     8281 ctgctggcac ttgaagaaac cggcagcgtc tcccttgccg ccaaacgggt tttccttacc
     8341 caatctgcac tttcccacca aatccgtatg cttgaaaacc actacggcgc accgttgttc
     8401 gaacgaaaat ccacgccctt gcgctttacc ccggttggag aaaggctgtt acgcctcgct
     8461 cacgacctgt tgcctcaagt taccgatgca gaacgtgatt tggcacgcat tatagaagga
     8521 gaggcaggag aattgcggat tgccgtcgaa tgccatacct gtttcgactg gctcatgccc
     8581 gcaatgggcg aattccgccc gatatggccc caaatcgaat tggatatcgt atcgggattc
     8641 caagcggacc ctatcggact gctgctgcaa caccgcgccg atcttgccat tgtttccgaa
     8701 gcggaaaaac aaaacggtat ttctttccaa ccgctgtttg cctacgaaat ggtcggcatc
     8761 tgcgccaaag atcacccgct tgccgccaaa actgtctggg aagccgaaga ctttatcggg
     8821 gaaaccctga ttacttatcc cgttcccgac gagatgctgg atttgcccaa aaaaatcctg
     8881 attccgaaaa acatcaaccc gccgcgccga cacagcgaac tgaccatcgc cattatccaa
     8941 ctggttgcca gcagacgtgg cattgccgcc cttccctatt ggacagtcat gccctacctt
     9001 gaaaaaggct atgtcgttca ccggcagatt actgccgacg gattgcaaag caaactgtat
     9061 gccgccatcc gtaccgaaga tacggacaag agctatctga acaatttttg ccaaatcata
     9121 cgcgaacgcg gttttgcaga tttgcccgga ctgagcgaac tggaaccggt ctgacccctt
     9181 attcaaccat acccggcagt ttttctattt tttcatgtat agtggattaa caaaaaccag
     9241 tacagcgttg cctcgccttg ccatactatt tgtactgtct gcggcttcgt cgccttgtcc
     9301 tgatttttgt taatccacta tactgttttt gatttttgcc caatctgtaa tctttagatt
     9361 gccaatggga aaccgtctac tacaaataaa aaaaccctgc gataagcagg gttttttgaa
     9421 tttccaacat taacgtttgg agaattgttt tgcacggcgt gctttgcgca gacccggttt
     9481 tttacgttcg acttcgcggg catcgcgggt aacaaaaccg gcttgagaca aggcgggttt
     9541 caacgcggca tcgaagtcga tcagggcacg ggtaatgccg tggcggattg cgccggactg
     9601 gccggtttcg ccgccgccaa caacattgac tttgatgtcg aaagattcgg cgttttcagt
     9661 cagaaccaag ggttggcgaa caaccattcg gctggtttcg cgtgcgaaga attcgtcaac
     9721 gggacggccg tttacgatga tttgacctgt acctttaatc aggaatacac gagccactga
     9781 acttttgcgg cggcctgtgc cgtagtagta tttaccgttc atgtcgcgtc cttatttcag
     9841 ttccaaaact ttgggttgtt gcgcagcatg ggcgtgttcc gcacccgcat acactttcag
     9901 ttttttaatc atggcgtaac ccaaaggacc tttgggcagc atacctttta cagcttgttc
     9961 caaagcgcgg cccgggaatt gctcttgcat ttcgcggaaa gtgcgttcgt agataccgcc
    10021 tgggaaaccg gaatggcgga agtatttttt atcttcgaat ttggcaccgg ttacacgcag
    10081 tttgtccgca ttgataacaa tgatgtaatc gccggtatcg acgtgggggg tgtattcagg
    10141 tttgtgtttg ccacgcagac ggctggcgac ttcggccgca acgcgaccca agactttgtc
    10201 ttgggcatcg atgacgaacc attcgcgctt cacctcgtgg ggtttcgctg aaaaggtttt
    10261 catagtggaa atccaaatag atatagaaag ttgtaaattt taaagacagg attcgatttt
    10321 gtcaatcgca ttaccgcgtt acggaaggat tttccggatt tcggcagact gcatactgct
    10381 ttttcgggcg gggcggcggc caatgtgaaa aaccgcatcg ttgcgatgcg gttttgaatg
    10441 ggaatccccg cgagagccgt ttcggccgaa tccgcttgaa ccttgctgac aaggcggctg
    10501 cctcgggtag tttcgggtgc gtccgcaaaa ggacgctcgc gcccactact gctcccggca
    10561 accttaagcg aacttattgg ttcaaaggaa tatatgcctt cgcggacacc gcagggaaaa
    10621 aggggttatt cctgcgccaa gcgggatagt gctttttggc aggcgttgtc catatcggct
    10681 attttacgcg caaaatcgcc gattgccaaa tcgccgccgt tcagggaggt tttcaacagg
    10741 tcgtggacga cgttgagcgc ggccataatg acgatttttt cgctgtccgc gacgcgtccg
    10801 ccttcgcgga tggcttcggc tttgccgttg agcattccga ctgcctgcaa cagtgtgtct
    10861 ttttcttctg ccggcgtgtt aacggtcagc cgggcgttca tgacttcgat gtggacttgt
    10921 tcgatgttca tcctttaatc cttattgctg cgtttcctgc cattggggga ggcgcgctgc
    10981 cagtgcgctg attttttccc tgctctgttc gagcaggctg cggtatcgtg tattttcttc
    11041 tgtcaggctg tcgattttgt tttgcaggtc ttctttgagt ttgccgactt ggacgagcag
    11101 ggcttcgctg agttcgtcga cggcggtttc gtgttcgagt ttttgccgct cgtgcgcccg
    11161 tttgagttcg gcgacggttt ctttgaggcg gcggttttcg ctgacgaggg tttcgaattt
    11221 ttgtaccagc gtataaacgc tgctttcgag tttttcgata ttttgtttca taaccttacc
    11281 tgtccgtatg ccgtctgaag gcttcagacg gcatctgtct gttgtttatt cgaaacgcgc
    11341 gctgcgttcc atcagtcttt cgacgacctg ctgaggggtc atttccttac ggatgagttg
    11401 cagcagggtt tgggtaatcg gcatatcgat ttggtactta caggcagtat tgaagacttc
    11461 ttctatcgtg ctgacccctt cggaaacgtg tccgatttcg accagcacct gatgcagttc
    11521 cttgccttct gccaaaccca agccgacgcg gcggttgcgt gagagtgcgc cagtgcaggt
    11581 caagatgagg tcgccgatgc ctgccagccc catcatggtt ttgggctgtg cgcccattgc
    11641 ggaggcaagg cgggtaattt cagctaatcc gcgcgtaacc agtgcggcac gggcgttaag
    11701 cccgtactct aggccatcgg acaatccggt ggcaatcgcc ataacatttt ttaccgcgcc
    11761 gccaaccgcc acgccgataa cgtcggtact gccgtaaagc ctcatgacag tcgtgttgag
    11821 ctgcggtacg agttcttcaa tccactcttg gttttcggag gcgaggacga cggcacaggg
    11881 cagttgtttg gcgagttcct gtgcaaaact cgggccggag agtacgccga ttttcttatt
    11941 gtcgggcaat acttctttca atacttgaaa ggtcagcagc cctgtatcct gctcgaatcc
    12001 tttgcaggcg gcgaggacgg ggaggtgtcc cgcgccgtac tgtttgagca gctctgcgct
    12061 gcttctcaat ccggcaacgg aggttacgat aaggacaagt ccgctgtctt tgagcgcgtc
    12121 tgccaaatcc gcacacactt ccaaggtttc gggaaaggaa aagccgggca gtccgcgttt
    12181 gttttcacgc gcttcctgca tttggcggac ttggtctgcg ttgcgcgtcc acagggatac
    12241 gcggttgccg tgttgggaaa agtgcagggc gagcgccgta ccccacgaac ctgcgccgat
    12301 aacggtaatt ttcattggtc gtctttcaac atatcactgc cgttcacttt aaaacaatcg
    12361 gtgtttctct gcaagtgcgg tcagggaaat gccgtctgaa aggtgttcag acggcatttt
    12421 gccccgatgc ggcacaatca gcctgtattg cgcaaacctt gcgccacgcc gttgatggtc
    12481 aggtgaacca tcagcagcgc gtgcggattg tcgggttctt tacgcaggcg tttgagcatg
    12541 gcgacctgca ggccgttgag cgcgttcaaa tatggaatcc tcaaggcaag cgaacgggcg
    12601 aggctgcggt tgtcgcgtaa aagttcttcg gtttgcagca ggtcgagcag ggctttgcgg
    12661 ctgcgttggt attcttcctt aatcatcccg aagatgattt ttgccttatc gggcgattcg
    12721 ctcaagccgg catagttttc cgcgagggtg atgtcggttt tcgccatcac ttgctccata
    12781 ttggaaagca tggcttggaa gaacgggttg ctttgggcgt gttcgcgcag ggcggcgagc
    12841 gtttcgggtt tgtcttcgca caaggtttcc accgcgctgc cgaaaccgta ccaagcaggc
    12901 agcatgaggc ggttttgcat ccatgagaat acccacggaa tagcgcgcaa gtcctgaatc
    12961 cgcgccaagg ttttgcggct ggcaggacgg ctgccgaggt tgagggtggc gatttcctga
    13021 atcgggctgg tttgcaggaa gtagtcgatg aagtcggggt gggtaatcag ttcgcggtag
    13081 tatttgaacg atacgtccga caatgcctgc atcagtttgg catcagggtc ttttttatcc
    13141 ggcaggatgc tggcttccaa agtcgcggca accaaggttt ccaagttgcg ttgggcattg
    13201 ccggggtcgg cgtatttggc ggtaatgact tcgccttgtt cggtaatgcg gatttgtccc
    13261 gccacgctgc ccgccggttg ggcgagaatg gcttggtaag aagggccgcc gccgcgtccg
    13321 acgctgccgc cgcgaccgtg gaacaggcgc atgcggacat cgtatttttt gaagagttcg
    13381 accaagccca attccgcctg atacagacac catgagctgg taacatagcc gccgtcttta
    13441 ttggagtcgg agtagccgag cataatttct tggatgttgc cgcagctttc gagcagggca
    13501 tcgtaccaat cgaggcggaa catggtttcc atgaccggac aggcgttttc gagtgcttcg
    13561 atggtttcaa acagcggcac gatattgatg cggctgcgcg gtttgccgtt ttccaccgcc
    13621 agcaggccgc tttctttcag cagcaatgcc aaggcgagca ggtcgctggg ttgttcgcag
    13681 ttggaaataa tgctttgtgt aacggcatct tcgccgaatt cgtctttgat tttgcgcgct
    13741 tcgttgaaaa ttgccagttc gtggcgggta tggtcgctgt atgtgatgaa cgggctgtac
    13801 agcggacgtt gatggctcaa ttcgcgcaac agggcggctt gtttttgctc ttcgttcagg
    13861 ctgttgtagt cttccaagcc tgcgtgttgg aaaagctcgg caaccacatc ggcgtgtttg
    13921 cctgcgtgtt ggcgcaggtc gagcggcatc atgtggaagc cgaacacgga cacggaacgg
    13981 atgaggtctg ccaaacggcc ttcagcaagc aggcggctgc cgttgtcgat aagggaacgt
    14041 tgcaattttt tcaaatcatc gagaaattct tgtgccgaag catacggctc gagaaagccg
    14101 aatttgcagc ccatgcccaa accgagcgcg cgcgctttgc ccatagcgcg cgccatgatg
    14161 taagcaatgg cgcggcggta gggttcttcg gtgcgagcga tttcttcgtc aggcgagagg
    14221 gctgccagtg ccattacgtc gtcgttgact ttgacgcggc ggatggagag cggcagttcg
    14281 cggtaaagtt tgtcgagttc gccacggtaa aaacggaaca cggcatcggc gtggcggcgg
    14341 aaggcaaaac gcaaggtttc ggcagaaacg aacggattgc cgtcgcggtc gccaccgatc
    14401 cagccgccga ttttgaggat gttcggaacg cagacatcgg gatagaccat ctgaaagtcg
    14461 tgttccatat tgcggtagag cttgggcagg gcttcgaaaa agctcatcgg gaagatggac
    14521 acgccgttgt tgatttcgtc gttgacgctg agtttgtggc ggcgcgtttc gctggtctgc
    14581 cacaagccca acaggatagt gtcaatttcg cggcgcagcc gtgccagcgc gtcggcattg
    14641 gtgcagcgtt cgcgttgcgg caacagtgcg cggatgcggc ggttgaagct taagacggtt
    14701 tggcgttgca cttcggtcgg gtgcgcggtc aaaacggcgg taacggacgt attgtccaac
    14761 tgccgctgca ccgatttgcc gtcggctttc cccgctttga gcctgcggac ggtttccgtc
    14821 aggctgcctt ccgcgccgcc gcgtccggct tcttcgtgga tttggcggcg gcgttcgtgg
    14881 tgcacgtctt cggcgatatt caaaatctgg gcgaacaggc cgcaggccaa ggttaaatcg
    14941 tgggtttgtt gttcgtccaa ttgcggcaat actttttcaa tcaatgccgc gctgtcgtcg
    15001 gaagtggaca agagtttgac cgtttcgaca accaacggcg aggcttcttc gtgcaggagg
    15061 ttgaacaggg attgtttcag aaattccgcg tccgccgcca aagccgcgtc cttcggattg
    15121 ttcagaatat gcagttgcat gatttttctc tcattgccgt aaatactgta aatgtacctc
    15181 aaatgccgca tccgtgccaa accgttcaca ctttaaccgc ccgtgtcccg aaatgccgtc
    15241 tgaagttgaa cgccgcccga cggcagcgtt acaatcgccc gcaactgttt tttccgaaca
    15301 tcatcatgac cacgaccgaa cacgacaacg acgacgaact cctgctgcgg tacagccgcc
    15361 acatcctctt ggacgaaatc ggcatcgaag ggcagcagaa actttccgcc gcgcatattt
    15421 tggtcgtcgg ctgcggcggt ttgggtgccg ccgccctgcc ctaccttgcc gcttccggca
    15481 tcggcacgct gaccatagcc gattccgaca cggtcgagct gcacaacctg caacgccaag
    15541 tcgcatttga cgagggcgat gtcggcaaac tcaaaaccga agccttggca gaccgcctga
    15601 aacgcatcaa ccataccgtc aacgtccgcg ccgtcaacga aaaactcgac ggctgccgcc
    15661 tgaccggttt ggttcaagcc gccgacatcg ttttagactg ctgcgacaac tacgccacgc
    15721 ggcaagccgt caaccgtgcc tgcgtacaaa cgaaaacacc gctggtttca ggggcggcgg
    15781 tacgctttga agggcaactt gccgtgtacc gtcccgactt gcccgactcg ccgtgttacg
    15841 cctgcctgtt tgacggcgga tcggcttcag acggcatctg ttccctcttc ggcgtattct
    15901 cgccgctggt cggcatcatc ggcagtaccc aagcggcgga ggctctgaaa atcctgctgg
    15961 atgcgggcga accgtcgcac ggcaggctgg cggtttaccg tgccttggaa gggggctggc
    16021 aatatttcga cctgccgcgc aaccccgaat gcccggtttg cggcgcggaa cggtaaaacc
    16081 ctgccgccgt ttcagacggc atccaaacgg atgcggagga acggttttaa aaatttaaaa
    16141 atttacattt ctttgcaaaa aaaataaact taccttataa ttgcagttgt tttagcaatg
    16201 tctgtttcgc agactcattg agtaagacgt tttccccgta atgtgtttgg ccgtctgtcc
    16261 cctttgggtt cggacggctt ttttttggct gtgtatgaat acccggttgg ttttatctgt
    16321 ttacagggga aaaccgctta tttccgttcg ggcggaaaac ggttccatcg gataaaaggc
    16381 attttgtccg actgattaaa g
//
LOCUS       DAXIEB010000040        15858 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000040 DAXIEB010000000
VERSION     DAXIEB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.040"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(84..308)
                     /locus_tag="ACFOA3_001025"
     CDS             complement(84..308)
                     /locus_tag="ACFOA3_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6898049.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(309..1697)
                     /locus_tag="ACFOA3_001026"
     CDS             complement(309..1697)
                     /locus_tag="ACFOA3_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6898050.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLVFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1783..2604)
                     /gene="prmC"
                     /locus_tag="ACFOA3_001027"
     CDS             complement(1783..2604)
                     /gene="prmC"
                     /locus_tag="ACFOA3_001027"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6898051.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRTSDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIASNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(3119..4603)
                     /gene="tldD"
                     /locus_tag="ACFOA3_001028"
     CDS             complement(3119..4603)
                     /gene="tldD"
                     /locus_tag="ACFOA3_001028"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6898052.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4781..4884
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4950..6173
                     /gene="cytX"
                     /locus_tag="ACFOA3_001029"
     CDS             4950..6173
                     /gene="cytX"
                     /locus_tag="ACFOA3_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6898053.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLNAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            6170..7270
                     /locus_tag="ACFOA3_001030"
     CDS             6170..7270
                     /locus_tag="ACFOA3_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6898054.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRNIRGHLKTPAMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7291..7908
                     /gene="thiE"
                     /locus_tag="ACFOA3_001031"
     CDS             7291..7908
                     /gene="thiE"
                     /locus_tag="ACFOA3_001031"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6898055.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNAQAVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
     gene            8330..8524
                     /gene="thiS"
                     /locus_tag="ACFOA3_001032"
     CDS             8330..8524
                     /gene="thiS"
                     /locus_tag="ACFOA3_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6898056.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
     gene            8738..9526
                     /locus_tag="ACFOA3_001033"
     CDS             8738..9526
                     /locus_tag="ACFOA3_001033"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6898057.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(10289..11113)
                     /locus_tag="ACFOA3_001034"
     CDS             complement(10289..11113)
                     /locus_tag="ACFOA3_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6898058.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAQAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADAEEIRKITSLYGKLNLKSCK"
     gene            complement(11226..13004)
                     /locus_tag="ACFOA3_001035"
     CDS             complement(11226..13004)
                     /locus_tag="ACFOA3_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6898059.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVSGVDEKGILHLDTGDGRQTVVSGEISLRPDDRPVSVPKK
                     REPERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            complement(13001..13507)
                     /gene="rfaE2"
                     /locus_tag="ACFOA3_001036"
     CDS             complement(13001..13507)
                     /gene="rfaE2"
                     /locus_tag="ACFOA3_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC6898060.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(13718..13793)
                     /locus_tag="ACFOA3_001037"
     tRNA            complement(13718..13793)
                     /locus_tag="ACFOA3_001037"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13758..13760),aa:His,seq:gtg)
     gene            complement(13821..13897)
                     /locus_tag="ACFOA3_001038"
     tRNA            complement(13821..13897)
                     /locus_tag="ACFOA3_001038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13861..13863),aa:Arg,seq:tct)
     gene            complement(13922..13999)
                     /locus_tag="ACFOA3_001039"
     tRNA            complement(13922..13999)
                     /locus_tag="ACFOA3_001039"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13962..13964),aa:Pro,seq:tgg)
     gene            14222..15076
                     /gene="folD"
                     /locus_tag="ACFOA3_001040"
     CDS             14222..15076
                     /gene="folD"
                     /locus_tag="ACFOA3_001040"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6898061.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(15138..15719)
                     /locus_tag="ACFOA3_001041"
     CDS             complement(15138..15719)
                     /locus_tag="ACFOA3_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6898062.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
BASE COUNT         3549 a         4782 c         4175 g         3352 t
ORIGIN      
        1 ttaatccact atatatctta ggtttgcatc ggcaaaatat tcaaacacag ccttttttaa
       61 ggaaatccgg atacggcggc acatcaataa tgcggcggaa tctcgtcgcg cagggaatac
      121 ggctcttgcg tgtcgggatt cctgtcctgc attttttgat acagcagcct caactgagcc
      181 tgctgcaaat ccagcgtctg ccgcaattcc gccaccatcg cgttcaggcc ggcgattacg
      241 tcctcctgaa gcgcggattg gatttccagc tctgtgatgc ggtgttccaa ctcttgaacc
      301 gcgtccattt acagcaccat cgcggcaatc cagccggcaa tcagcagcgg gatattgtag
      361 tggatgaagg tcgggataac ggaatcgcgg atgtggtcgt gctgcccgtc ggcgttcagc
      421 cccatcgtcg ggcccagcgt ggaatcggac gcaggcgaac cggcatcgcc caacgccccc
      481 gccgtgccga caatggcgac ggtggcaagc ggcgaaaaac ccaaaccgac acacaaaggc
      541 acataaatcg cggcaataat cggcaaagtg gaaaaggacg aaccgatgcc catcgttacc
      601 aaaagcccca ccaccagcat cgccaacgcc gccatacctt tgctgttgcc gaatatcgcc
      661 atactgcttt ccaccagcgg ctgaatatgc ccggtcgcat tcatcacggc ggcaaaaccc
      721 tgcgcggcaa tcataatgaa gccgaccatc gccatcatct tgatgccttc gccgaatacg
      781 tcgtttgcct tgtcgcggtt gatgacgccc aacatcataa atacggcgaa accgagcatc
      841 gcgcccaaca ccagcgagtc ttcatacatc aactggatgg caaagcatac ggcaatggcg
      901 gcggcggcgg ccaggctgcg gtaggcggac ggctgcggac ggtttgccgc atcggcgttg
      961 cccgccgtat cggcattgtt gctttggtac aggcgcggtt tgcggtaatg gacaaacacc
     1021 agcaggagtc cggccagcat tcccaacgcg ggaatcgcca ttgccgccat tacgttgatg
     1081 tttttcacat caagctgcgg cgcggcggaa tggatgttgc ccaacaggat ttcattcaaa
     1141 aaaatcgcgc cgaagccgta aggcaggaac atataagtcg taaccagccc gaaagtgatg
     1201 acgcacgcaa tcaggcggcg gtcgattttc aggcggttga acaccaaaag cagcggcgga
     1261 acaatcatcg ggataaaggc gatgtggatg gggatgatgt tctgactcat aatgcccatc
     1321 acaaggatga tggaaagcag cagccatttg accgcgccct cgcccgaacg cacgctgtcg
     1381 ggcataccgc cccggttcag cttgcggacg accgcgccgg caagctgttg cggcaggccg
     1441 gaatgggtaa tcgccattgc aaacgcgccg agcatcgcat aagaaagcgc aatcttcgca
     1501 ccgccttcca aacctttgtt gaacacgggg ataatccccg cctgactgac ctgtcccgcc
     1561 gcatcggcaa tgttttgcag cggcataccc gccaccgcgc cgccgacaaa cgcgccgacc
     1621 gtcaggctca ataccacgtg cacgcgcgac agcgacagca ccagcataac gattacggca
     1681 actacgactg cattcattgt ttatcgctcc aaacctataa atgtttacat atcgaaacac
     1741 atcataaccc aataatggaa aacccgccaa ttttgcaaac aattatttca aatgcttcat
     1801 atacttcccc agcgtaaccc tgtccaaacc cgccaagtcc ggcagggttt ccactcctga
     1861 aaaaccattc tccgccaaca cgccgcgcac cgccgcgccc tgatcgaaac cgtgttccaa
     1921 caacaaaaaa ccgccttccg ccaaacggtc gggcgcgcct tgcgccaagg tgcggatgca
     1981 gcttaggccg tctgaaaagt cggtcagcgc gatttgcggc tcaaaccgca aatcgccttg
     2041 caacaaatgt ttatcgccgt tttcgatata gggcgggttg gacgcgataa tgtcccattt
     2101 cccttcagac ggcatatcgg tgtcgaacca cgaaccgtgt gcaaattcga cccgcgcgcc
     2161 caaatccgcc gcatttttcc gcgccgtttc aagggcgggc gtgctgatgt cggatgtgcg
     2221 cacaaacgca tcggggcgtt cgagtgcgac ggttacggca accgcgccgc tgcccgtccc
     2281 caaatcccac acgcgcccgt tttcgggcag gcgcgccaat acggcttcga ccaaatgttc
     2341 ggtttcgggg cgcggaatca gcacgctcgg attgactgta aagcgtctgc cataaaattc
     2401 gcgcacacct aaaatatagg caaccggctc gccgttcaga cggcgttgcg ccagcctgtc
     2461 cgcccgctgt cggacttcgt ccggcatttc ttccccgccc cgcgtcaaca actgcacgcg
     2521 cgtatattcc gaaacatatt gcagcagcat tcttgcttca tttttaggca gttttgacaa
     2581 gcccaaccat ttatcaaacg tcatttttat cccgtctgcc gctgatgcgg cttttctttc
     2641 cttattcttt ccggcaaacg taccgatggt ggcaaccgca aatgcggcat accacaaata
     2701 aaatcctgca ccgtagcgca caatatccga tgtattccct gcttcatcga cgtatacggc
     2761 tttcacactg aaagccacca acgccaagcc ccaaagtgcc gcatggacag gcacgacctt
     2821 cttccgcaac gccagcaaaa caatggccgc caaccaaaca taattcgcat agaccgcaca
     2881 atacctgata tccaaagaag caaatatcga ccccaaaatc aaaacggtca aaccctccat
     2941 gcttccatga ttgcccaaat aaaatgcaac attggataaa gacgctatcc acagggcaac
     3001 cgacaccagc aacatcacta tgggaaaact tggtttccga ttctgttcct gcatggtttt
     3061 atcctaatgt aaaaggccgc ctgaaaacct ttcagacggc atcgtgccgg attccgcgtc
     3121 agattgcgct gccgccgacg gtcagtccgg catcaatccg caaagtcggt tgccccacgc
     3181 cgacggggac gctctgccct tctttgccgc acacgccgac accgctgtcg agcgcagtat
     3241 cgttgcctat catggaaacg tgtttcagca cttcggggcc gttgccgatg atggtcgcgc
     3301 ctttgacggg atattgcagc ctgccgcctt ccacccacca cgcttcggac gcactgaaca
     3361 cgaacttgcc gctggtaatg tccacttgtc cgccgccgaa attgacggcg taaatgccct
     3421 tgtcgatgga cgcgatgatt tcttccggct catagctgcc gttttccata aaggtattgg
     3481 tcatgcgcgg cataggggcg gaagcgtaac tttcgcggcg gccgttgccg gtggactgcg
     3541 tacccgtcag gcgggcattg gtttcgtcct gcatatagcc gactaaaatg ccgtcttcaa
     3601 tcaatacggt gcggcgggtt tcgttgcctt cgtcgtcgat gttgagcgaa ccgcgccggc
     3661 cggcaatatc accctgatcc acaacggtaa cgcctttggc ggcgacgcgc tcgcctattc
     3721 tgccggaaaa gacgctggtt cccttgcggt tgaaatcgcc ttccaaaccg tgtccgaccg
     3781 cttcgtgcag caacacgccc ggccagccgt tgcccaaaac gacggtcatt tcgccggcgg
     3841 gcgcggggcg ggattcgagg ttggtgagtg cctgtttgac ggcggcatcg acaaaccgat
     3901 gaaccaagtt ttcatcgaaa taagccaagt cgtagcgtcc gccgccgccc gcgctgccct
     3961 gttcgcggcg ttcgccctgt ttggcgataa cggtaacgtt caggcgcacc atcgggcgga
     4021 tgtcggcggc gtgtttgccg tccagacggg cgaggtaaac catatcgtat tcgcaggtca
     4081 aaccggccat cacttgcacg atgcgcggat cggcggcttt ggcgattgct tccactttgt
     4141 tcaacagcgc gactttggcg gcggaatcga ggccggcaat ggggtcggac gcggaacaaa
     4201 ccggcttgcc gcgcgtttca gacggcattt tggcggacac cttgccgcct gccgccccaa
     4261 tcgcgcggac ggcgcgggcg gaacggttta tcgaatcgat gcacaggctg tcggcgtagg
     4321 caaaggcggt tttgtcgccc gaaacggcgc gcacgcccac gccctgattg atttggaagc
     4381 tgcccgattt gacgatgccc tcttccaaat gccagctttc ataagcggtg cgctggcagt
     4441 agatgtcggc gtaatcgacg tggtgcgcgc cgatgatgca caggcttttg gcgagcagtt
     4501 cgggggaaag gcggttggct tcgagcagcc gcgcctgtac ggcggaatag gtcggatgca
     4561 tagtgtcggc gcataaaaaa tcaggggctt gattatacgg catttgttat atagtggatt
     4621 aacaaaaaac agtacggtgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc
     4681 gtcgccttgt cctgattttt gttaatccac tatagaaatg cgccgtgccg cctgaaatgt
     4741 aagatttttg ccaacgcccc ctgcttttgt gtacacttaa agctccttgt cggagtgccg
     4801 ccgccgggcg gctgagattg cgaaagcaga atccgtagaa cctgtcgggg taatgcctgc
     4861 gtaggaaaca aaacctcccc gccctttcgg gcttccgttc ccttttccgc acttccccgc
     4921 cccattttca tgttttttaa ggacttgata tgtcgggcaa tgcctcctct ccttcatctt
     4981 cctccgccat cgggctgatt tggttcggcg cggcggtatc gattgccgaa atcagcacgg
     5041 gtacgctgct tgcgcctttg ggctggcagc gcggtctggc ggctctactt ttgggtcatg
     5101 ccgtcggcgg cgcgctgttt tttgcggcgg cgtatatcgg cgcactgacc ggacgcagct
     5161 cgatggaaag cgtgcgcctg tcgttcggca aacgcggttc agtgctgttt tccgtggcga
     5221 atatgctgca actggccggc tggacggcgg tgatgattta cgccggcgca acggtcagct
     5281 ccgctttggg caaagtgttg tgggacggcg aatcttttgt ctggtgggca ttggcaaacg
     5341 gcgcgctgat tgtgctgtgg ctggttttcg gcgcacgcaa aacaggcggg ctgaaaaccg
     5401 tttcgatgct gctgatgctg ttggcggttc tgtggctgaa tgccgaagtc ttttccacgg
     5461 caggcagcac cgccgcacag gtttcagacg gcatgagttt cggaacggca gtcgagctgt
     5521 ccgccgtgat gccgctttcc tggctgccgc ttgccgccga ctacacgcgc cacgcgcgcc
     5581 gcccgtttgc ggcaaccctg acggcaacgc tcgcctacac gctgaccggc tgctggatgt
     5641 atgccttggg tttggcagcg gcgttgttca ccggagaaac cgacgtggca aaaatcctgc
     5701 tgggcgcagg tttgggtgcg gcaggcattt tggcggtcgt cctctccacc gttaccacaa
     5761 cgtttctcga tgcctattcc gccggcgcga gtgcgaacaa catttccgcg cgttttgcgg
     5821 aaacacccgt cgctgtcggc gttaccctga tcggcacggt gcttgccgtc atgctgcccg
     5881 ttaccgaata tgaaaacttc ctactgctta tcggctcggt atttgcgccg atggcggcgg
     5941 ttttgattgc cgactttttc gtcttgaaac ggcgtgagga gattgaaggc tttgactttg
     6001 ccggactggt tctgtggctt gcgggcttca tcctctaccg cttcctgctc tcgtccggct
     6061 gggaaagcag catcggtctg accgcccccg taatgtctgc cgttgccatt gccaccgtac
     6121 tggtgcgcct tttctttaaa aaaacccaat ctttacaaag gaacccgtca tgacccgtat
     6181 cgccatcctc ggcggcggcc tctcgggaag gctgaccgca ctgcagcttg cagaacaagg
     6241 ttatcagatt gcacttttcg ataaaggctg ccgccggggc gaacacgccg ccgcctatgt
     6301 tgccgccgcc atgctcgcgc ctgcggcgga agcggtcgaa gccacgcctg aagtcatcaa
     6361 actgggcagg cagagcatcc cgctttggcg caacatcaga ggccatctga aaacgcctgc
     6421 catgatgcag gaaaacggca gcctgattgt gtggcacggg caggacaagc cattatccag
     6481 cgagttcgtc cgccatctca aacgcggcgg cgtggcggat gacgaaatcg tccgttggcg
     6541 cgcagatgaa atcgccgaac gcgaaccgca actcggcgga cgtttttcag acggcatcta
     6601 cctgccgacc gaaggccagc tcgacgggcg gcaaatattg tctgcacttg ccgacgcttt
     6661 ggacgaactg aacgtcccct gccattggga acacgaatgc gtccccgaag gcctgcaagc
     6721 ccaatacgac tggctgatcg actgccgcgg ctacggcgca aaaaccgcgt ggaaccaatc
     6781 ccccgagcac accagcaccc tgcgcggcat acgcggcgaa gtggcgcggg tttacacacc
     6841 cgaaatcacg ctcaaccgcc ccgtgcgcct gctgcacccg cgttatccgc tctacatcgc
     6901 cccgaaagaa aaccacgtct tcgtcatcgg cgcgacccaa atcgaaagcg aaagccaagc
     6961 ccccgccagc gtgcgttcag ggttggaact cttgtccgca ctctatgccg tccaccccgc
     7021 cttcggcgaa gccgacatcc tcgaaatcgc caccggcctg cgccccacgc tcaaccacca
     7081 caaccccgaa atccgctaca gccgcaaacg ccgcctcatc gaaatcaacg gccttttccg
     7141 ccacggtttc atgatctccc ccgccgtaac cgccgccgcc gtcagattgg cagtggcact
     7201 gtttgacgga aaagacgcgc ccgaacgtga tgaagaaagc ggtttggcgt atatccgaag
     7261 acaagattaa agccgtctga aaggaacccc atgaccttcc cgccactcaa atccccgctc
     7321 aaattctacg ccgtcgtccc caccgccgat tgggtggggc gcatggtcaa agcaggtgcc
     7381 gacacggtgc aactgcgctg caaggccctg cacggcgatg aattgaaacg cgaaatcgcc
     7441 cgctgtgtcg ccgcctgtca gggcagccgt acgcagcttt tcatcaacga ccactggcac
     7501 gaagcgatag aagcaggcgc gtacggcgtg catctcggac aagaagacat ggacaccgcc
     7561 gaccttgccg ccatagccgc cgccggtttg cgcttgggtt tgagtacgca ctccgttgcc
     7621 gaactcgacc gcgccctgtc cgtacaccct agctacgtcg ccagcggcgc gattttcccg
     7681 accacgacca agcaaatgcc caccgccccg caaggcttgg acaaactgtg cgagtatgtg
     7741 aaacaggcag gcggcacgcc cgtcgtcgct atcggcggca tcgatttgaa caatgcccaa
     7801 gccgtactcg ccaccggcgt ttcctcactc gccgtcgtcc gcgccgtgac cgaagcggca
     7861 aatcccgaag cggtggttaa agcgtttcag gctttgtggg atggataaaa ccgaaagaag
     7921 aaaattcaat tgccgtgtag gcaaaactta gcccgttatc gcaaacatac ttaactttaa
     7981 atgtggcata tcatcaaatt ccgtcatttc cgcgcaagcg ggaatccgcc ttaaaacttg
     8041 agaaaccatc atttgaaaaa cagtttccga atttcaaaaa tggattcccg cctgcgcggg
     8101 aatgacggca actggccggt tgcgtatcaa aaaataaagt aattcggcta gatatagtgg
     8161 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggaaccga
     8221 ttcacttggt gcttcagcac cttagagaat cgttttcttt gagctaaggc gaggcaacgc
     8281 cgtactggtt tttgttaatc cactataaat acagaaacat cgagaaacca tgaacatcat
     8341 cttaaacggc gaacccgccg aacttcacgg cacaagcgtt gccgacctca tcgcccaaac
     8401 cgcgccgcaa aagccctttg ccgtggcggt caacaccgtt ttcgtcccca aaggcgcgta
     8461 tgcggaaacg gttttaaacg aaaacgacaa aatcgatatc gtgcggccgg tggtcggcgg
     8521 ctaggcggtt ttgccttttc agacgacccc tgtccccaaa acaacgttat ggtggattaa
     8581 ctttaaatca ggacaaggcg acgaagccgc agacagtacg gatagtacgg aaccgattca
     8641 cttagtgctt cagcacctta gagaatcgtt ttctttgagc taaggcgagg caacgccgta
     8701 ctggtttttg ttaatccact atacaaagga acccattatg ctcaccctat acggagaaac
     8761 tttcccttcg cggctgctgc tcggcacagc cgcctacccg acccctgaaa tcctcaaaca
     8821 atccgtccga accgcccggc ccgcgatgat taccgtctcg ctgcgccgcg cgggatgcgg
     8881 cggcgaggcg cacggtcagg ggttttggtc gctgcttcaa gaaaccggcg ttcccgtcct
     8941 gccgaacacg gcaggctgcc aaagtgtgca ggaagcggta acgacggcgc aaatggcgcg
     9001 cgaagtgttt gaaaccgatt ggattaaact cgaactcatc ggcgacgacg acaccttgca
     9061 gccggatgtg ttccaacttg tcgaagcggc ggaaatcctg attaaagacg gcttcaaagt
     9121 gctgccttat tgcaccgaag acctgattgc ctgccgccgc ctgctcgacg cgggctgtca
     9181 ggcgttaatg ccgtgggcgg ccccgatcgg cacgggtttg ggcgcggttc atgcctacgc
     9241 gttgaacgtc ctgcgcgaac gcctgcccga cacgccgctg attatcgacg cgggcttggg
     9301 tttgccctca caggcggcac aagtgatgga atggggcttt gacggcgtgc ttttgaacac
     9361 cgccgtttcc cgcagcggcg atccggtcaa tatggcgcgc gcttttgcac tcgccgtcga
     9421 atccggacgg ctggcatttg aagccggacc ggtcgaagca cgcgacaaag cgcaagccag
     9481 cacgccgaca gtcggacaac cgttttggca ttcggcggaa tattgaaaaa ggcggcaaaa
     9541 atgccgtctg aaggcttcag acggcatcgc ggtccaaaac ggcggcggcc tgaaacggac
     9601 aaaccgccat tccccagcat cacggctttg tcggaaaaaa tggaaaaacc ggccgaaaaa
     9661 ccttgccgcc cgtcccgatg ccgcaaccaa cgaaacactc ggcctccacg gtgtgcaggc
     9721 tgccgcgcaa gccctaaata cggcaataga agaagcgttt tgttgttgtt tgaatacact
     9781 taaaaatacc taaagccgtc cgtaagcctt catccgcaac ggttttaccg cttttgcatc
     9841 cccgaatcca cgctcaaaca cccccgaatg acaaccctgt ccgcgccaaa tcggacggat
     9901 gttcaaacac ggcaggctta tttccgtcag gcacgaagcc ctcagctatg actgccgacc
     9961 ccgatttgtc cgacacaatg aaagtttgcc gacccgaatc acaaacatcg gcggacaggt
    10021 taatttgttt atttttcatc gtattacaaa aaatccgcat ttatttttaa atttttattg
    10081 ataattatta ttattagcgt ataatcaaaa ccactcggaa gtcgtccatt ccgaaccatt
    10141 aaacactgta ttccctcaca attcactttc acacttggag tcggcatata cgagacatac
    10201 attccctttt tatatatcag atactcaaaa ccgaaacgcc aaacccacct tcgcggtggg
    10261 tttggcgttt atcgtccggc tttcgcgcct atttgcaaga cttgaggttc agtttgccgt
    10321 atagggacgt gattttacga atttcttccg catcggcggc attcacgccg gtaaacaaaa
    10381 ccgtcatacg cgacacgctc aaagaatcgt cctgcctgtc ggcttcggca aagtgttcga
    10441 caatatgcgc cccgccgaat ccggcgccgg caagccggtt ttgcagggct tgggcgtttt
    10501 cccggctgtt gccgacaccc aaactcaatg cgccgtcaaa cggtatgggg ttgaaacctt
    10561 tggcagacag ctccgccgcc tgattttcgg catcggcgga aacgggcagg acgacgcggt
    10621 aggttttgtc ggcaggtttg gcttgggcgg tgcgtttttc gacgctcctg ctggcaacgt
    10681 gcgaccattt gcccaaaagt cctttgatgc ggtggtagtc atcttcgtcc atcgtgaggc
    10741 ttgcctgcgc ggcgcataag gcatccgccg cgccgttttc gcgttccgcc tgcgcctttt
    10801 cggcggcgag tttttcgcgg cgggcttttt cttcacgctg ttttttctcc ctcagttttt
    10861 tctgttccgc ttcctttttc aagcgcaact gctcggcctg ctcttcggtc aggatgtcgc
    10921 ccggtttgag cagtgccgcc gtatcctcca cggatgccgc cgcattgtcg gcggcgggcg
    10981 ttgcgggggg cgtgttttcg acggcccggt tttccggcac gccgcccgtc gctttaacgg
    11041 ctaatttata gcccaccgtg ccgccgaata cggcaatatt gagtaccgcc aaaagggtga
    11101 agagccattt cattagaatt tcctcaatgt ttgaaacccc gcccgcctgg gcggcagtct
    11161 gtcgggcggc agaatccgga cggattcgga cggatgccgc cccgtgttga aaaagggtgt
    11221 attttttaag tatgttccga ttccccgcct tcggcggcaa tcaggttcag cagcccgtga
    11281 atgacgaggt tgtccgccac gcgcacggta ttttccgcca aaaatgcagg cggcagggct
    11341 tcggcaactt ttgccgcgcc gccgccggta atgatgacat cgacaggctt gcccgccccg
    11401 gttttttctt tcaaacgccc gtgcatcatc ataaccgagc cgcaaaccgc atccatcatg
    11461 ccgctggcga cggcattgcc cgttgtggtc gggaaaggat aacgcttacc ggcgtgccgg
    11521 ttgaggttgg cggttcggac ggcgagcgat tctttcatca ggtggaaacc gggcatgatg
    11581 gttcccccga gataatgtcc gtcatcggtg agcgcgtcaa ccgttaccgc cgtgccgcaa
    11641 ctgacgacga cgcaggcgtt gcggctgaag cggcggctgc ccaaggcgtt gaaccagcgg
    11701 tcggaaccgt gttcttcggg gtggcggtag tggttgcgta tgcccaaagc ctgtgcggaa
    11761 gacggcagcc actcgatttt tcgggcgagc tgttcctgca cttgtgcctt tttgaattct
    11821 ccgcacacag cgcaaccgac gatgcggaca tttccatccg ccttttccgc ccactccgcg
    11881 cccaaaggcg acaaatcgcg gtacggcgcg ctaccgacgg ttgcgaacgt gccgttttcc
    11941 acccatgccc acttgagccg actgttgccg ccgtccaaca gcaaaaaacg ttccggttcc
    12001 cgcttcttcg gaacagagac cggcctgtcg tcgggacgca aactgatttc tccgctgact
    12061 acggtttgcc tgccgtctcc ggtatccaga tgcaatatac ccttctcgtc cacaccgctg
    12121 accgtgcctt cgaacacggt ttcgccgtcg cgcaacagca ataccgcctt gccgtggtcg
    12181 cggttggcag cctgatattc cgccacaaaa ggcgcaaatc cgtcccgcgc atattgcaac
    12241 aacaccgcgt ccagttccac caacagcgtt tccagcagca cggcggcatc ggcattgccc
    12301 cgccgcgatg ccgtctgaaa cagcgattgc acggaagcgg cattttctac ttccttgggc
    12361 aggacaaaat tgatgccgat accgaccacg gcaaccgttt tgccgcccgt cctgaccgtt
    12421 tcaatcagaa tgccgcccaa tttgtcgcgt ccgacaacca aatcattggg ccacttaatc
    12481 tgcacatcca aacctaaacg cgacaaggcg cgccgacacg ccactgccgc aacaggcgac
    12541 agcgaaccca gctcatattg cggccggtca aacacccagc caaaactgaa catcagacac
    12601 tcgcccaaac ggtgcgacca cttccgcccc tgccgccccc tgcccttact ttgcaggtgg
    12661 gtcacacata tggttttgtg cgccttgtcc ggcgcaatcc gcgccaattc cagtatctcg
    12721 tcgttgctgg acgcgcactc gtgcttcaat gccgtctgaa aacccgacct ttcccccagc
    12781 tcgcgcaaac cttcggcatc gaaaaccgcc aatgggcgca ccagccgcca atagccgtcg
    12841 tgttggcgca acagcccgcg tatgtgcgcc ggcatctgct gccaaaaacc gttgagctgc
    12901 tgcggcttca tatccgccat acgcgccagt tgcgagacgt gttgcggcaa accgtcggca
    12961 agctccgcca acacccgcca gtgcgaaagc ttcaaaaccg tcattttccg ccctctgccg
    13021 cacggatttt tgccaaagtc ttcgttgtcg aagtctggtg cagaaacgga attgaaaaca
    13081 cctgaccgcc gcgcgccaac gtttctgccg caccgacaat cttatccgca gcccaatcgc
    13141 cgcccttgac caaaatctca ggtttgaccg cctcaatcaa cgccgccggc gtatcctcgt
    13201 caaaccacgt taccaaatcc acactttcca acgcggcggc aacggcggca cggttctcca
    13261 aaggattaat cgggcggtcg ccgcccttgc ccaaacgccg caccgaagca tcggtattca
    13321 acgccagcac caacgccgcc cctgccgaac gcgcctgcgc cagataagta acgtgccccc
    13381 tgtggaggat gtcgaaacag ccgttggtaa acaccagcgg gcgcggcaac aacgccaaac
    13441 gcgccgccaa cgcctcgggc ggacagattt tcgattcaaa atcaggaaca gaccaagcgt
    13501 caaccatcaa agtctccgac aaaaaccata aaagacagaa aacctacatg atacagaagc
    13561 atatgcgaaa ggcaaagccg gcggcgcaaa tgccgtctga agcggtttcc gtatgaccgg
    13621 cccggctttc agacggcata ccgccgcacc ggtacgacgg cgcggacagt acgcgcaaac
    13681 ggaaaaagac ccgtaccgaa aagtacgggc ctttatctgg ggtggctgat ggggctcgaa
    13741 cccacgacaa ccggaatcac aatccggggc tctaccaact gagctacagc caccataaaa
    13801 acggttttca atcaaattct tggcacaccc gacaggaatc gaacctgtaa cccccgactt
    13861 agaaggtcgg tgctctatcc ggttgagcta cgggcgctca tgccgattcg tgctgattga
    13921 ttggtcgggg cggtgggatt cgaactcacg accctctgct cccaaagcag atgcgctaac
    13981 cgggctgcgc tacgccccga cttgaagaag cgaactatac aactcaggga aagatgcgtc
    14041 aatatttatt ttcaagacac caagatgaaa aatatagttt tttgatttga aaaaatattt
    14101 aatccgtcca aacagccgta ttttatttca gggcaaattt attttcggca tcctgctgta
    14161 aaaacaaacg gaaaatgcga taattttcag cattttctac ctgtttaaca aaaggacgga
    14221 tatgtcggca caactgatca atggtaaaga agtttcgcaa aaacgcctgc aggcggttgc
    14281 cgaagcggtg gcgcaacgcc aacaggacaa tctgcacatg ccttgcctgg ccgtggtttt
    14341 ggtcggaggc gaccctgcca gcgcggttta tgtccgcaac aagaaaactg cctgccaaaa
    14401 atgcggcatc aaatcactgt cttacgagct gcccgaatca acatcgcagg aagaactgct
    14461 ggcactggtc gaccgcctga atgccgattc cgaagtggac ggtattctgg ttcagctacc
    14521 gctgccgaag cacctcgaca gccaggcggt tttggaacgt atttcgccgg ataaggacgt
    14581 ggacggcttc catccttaca atgtcggcag gctggcggtc aaaatgccgc tgatgcgccc
    14641 gtgtacgccc aagggcgtga tgacgctttt ggaagcttac ggcattgatc cgaaggggaa
    14701 aaaagcggtc gtggtcggcg cgtcgaatat cgtcggccgc ccgcaggctt tggaactgct
    14761 gctggcgcgc gcaacggtaa cggtctgcca cagcgcaacc gaaaatctga cagacgaggt
    14821 tgccggagcc gatattttgg tggtcggcgt gggcattccg aactttgtca aaggcgaatg
    14881 gatcaaaccc ggcgcggtcg ttatcgatgt gggcatcaac cgtttggacg acggcagcct
    14941 gtgcggcgac gtggaatttg aaacggcaaa agaacgggcg gcgatgatta cgcccgttcc
    15001 cggcggcgtg ggtccgatga cgattgccac attgatggaa aacaccctgc acgcggcttc
    15061 actgcacgat gcttgagcgg ttctgaagat aaaaatgccg tctgaaaggc tttcagacgg
    15121 cattttgccg tgtccgttta tttgggcagc ttgacgacaa ccgtatccgc cagtatgtcg
    15181 taaagcgtgc ggcggtcgcg tttgaccata aagagcagga caaagttggc aaggaatgcc
    15241 agcaggttga tggcgttttc tccgttgtca cctactgcaa gaccgataac ggcggcaata
    15301 atggcaacca aaaccgacca tgcgatttcg cgtaccaaaa ccgtgccgac aaaaccggga
    15361 ttgcggccgt cggttttcaa cacgcggatt ctcatgattt tcttacccaa tgactgcccg
    15421 tcccgactca tatagtagat ttgaacgacg gtataaacca aaatgcccgc cagtcctacc
    15481 caaaaggaag tcatgcccaa aagcagcccg aatatttcct cactgccgcc aatcctgcct
    15541 tcattcttga tggcaaaagc aatcagtccg gcaaacggca ccaacaaaac caaaaaggta
    15601 aacaattggt tcagcagcgc ggcaagtatc cggtcgcctg cgccggcaat tccgacttca
    15661 atttcctgcc cgttgcggtt gtcggatgcc gcgtcggtgt agtcgttttt ttcttccata
    15721 tccgttcctg ataattgttc ttaactgacc ccgattctac cgccacgaca ccgaaaacgc
    15781 caatacttaa agaaatcccg ataaagaact ttacattttc ccaatacggc gttaaaacgc
    15841 ttcctttacg ccatacat
//
LOCUS       DAXIEB010000041        15288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000041 DAXIEB010000000
VERSION     DAXIEB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.041"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..230)
                     /locus_tag="ACFOA3_001042"
     CDS             complement(<1..230)
                     /locus_tag="ACFOA3_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898063.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKT"
     gene            complement(395..1114)
                     /locus_tag="ACFOA3_001043"
     CDS             complement(395..1114)
                     /locus_tag="ACFOA3_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6898064.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(1198..2511)
                     /gene="hpnE"
                     /locus_tag="ACFOA3_001044"
     CDS             complement(1198..2511)
                     /gene="hpnE"
                     /locus_tag="ACFOA3_001044"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6898065.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(2590..3462)
                     /gene="hpnD"
                     /locus_tag="ACFOA3_001045"
     CDS             complement(2590..3462)
                     /gene="hpnD"
                     /locus_tag="ACFOA3_001045"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6898066.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3633..5495
                     /gene="hscA"
                     /locus_tag="ACFOA3_001046"
     CDS             3633..5495
                     /gene="hscA"
                     /locus_tag="ACFOA3_001046"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6898067.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            5731..6381
                     /locus_tag="ACFOA3_001047"
     CDS             5731..6381
                     /locus_tag="ACFOA3_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6898068.1"
                     /translation="MSAARRLVLIFVNSLYKIGISEVSNFEGRMRHLENNGYANVAGL
                     ERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNET
                     AESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIR
                     LFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            6418..7179
                     /locus_tag="ACFOA3_001048"
     CDS             6418..7179
                     /locus_tag="ACFOA3_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6898069.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            7234..7575
                     /gene="fdx"
                     /locus_tag="ACFOA3_001049"
     CDS             7234..7575
                     /gene="fdx"
                     /locus_tag="ACFOA3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6898070.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(7862..8449)
                     /locus_tag="ACFOA3_001050"
     CDS             complement(7862..8449)
                     /locus_tag="ACFOA3_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6898071.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACLELEGRYLELVRRAAVSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            8662..8859
                     /gene="iscX"
                     /locus_tag="ACFOA3_001051"
     CDS             8662..8859
                     /gene="iscX"
                     /locus_tag="ACFOA3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6898072.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(8927..9328)
                     /locus_tag="ACFOA3_001052"
     CDS             complement(8927..9328)
                     /locus_tag="ACFOA3_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6898073.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            9539..10498
                     /locus_tag="ACFOA3_001053"
     CDS             9539..10498
                     /locus_tag="ACFOA3_001053"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6898074.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            10566..11876
                     /gene="tilS"
                     /locus_tag="ACFOA3_001054"
     CDS             10566..11876
                     /gene="tilS"
                     /locus_tag="ACFOA3_001054"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6898075.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            11927..12712
                     /locus_tag="ACFOA3_001055"
     CDS             11927..12712
                     /locus_tag="ACFOA3_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6898076.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            12722..12919
                     /locus_tag="ACFOA3_001056"
     CDS             12722..12919
                     /locus_tag="ACFOA3_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898077.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            13181..13534
                     /locus_tag="ACFOA3_001057"
     CDS             13181..13534
                     /locus_tag="ACFOA3_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898078.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            13546..13851
                     /locus_tag="ACFOA3_001058"
     CDS             13546..13851
                     /locus_tag="ACFOA3_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898079.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            13913..>15288
                     /gene="mpl"
                     /locus_tag="ACFOA3_001059"
     CDS             13913..>15288
                     /gene="mpl"
                     /locus_tag="ACFOA3_001059"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6898080.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQVGEVNADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKAALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
BASE COUNT         3567 a         3913 c         4355 g         3453 t
ORIGIN      
        1 atggtttttg cctggctgcc caaacggtct tccccgtcgg agaaaatgcc tttttggaag
       61 ctggagcggt tgtcgaatgt tccgacggaa atcggggtac gaacaccgtg atattgcgta
      121 ttgtaggagg cgactttctc tacctcgaga ctgcgtgagg attcggtcgc acagccggtc
      181 agtgaaacgg cagcggcggc aaggacaacg gcggtggaaa cggttttcat aaaatttacc
      241 ctaaggtcaa gttaaggaaa taacgggtta tcattattgt ccttatgtaa atttaagtca
      301 aggtgtttgt ctgtgcggga cggatgcgcg cggaaagtac ggatattttt caaacggaat
      361 cgcggcgcgg gcgggatgcc tgccttttcc ggatttagag gtaaacgatt tcgccgctcc
      421 gccctttgct ttcggtactt gcccaccaga caaatgcggg cagcacgtcc ccgtagcttt
      481 tgcgttcgct tttggcttcg cccggatggg atttgatgcg ttgcggggaa ttgatgggtc
      541 cggggacgag gacgttggcg cgcaggttgc cgaagcgttc ccattcgtcg gcggcgactt
      601 tgcacaggta gttcaacgcg gctttggacg cgccgaagcc gccccagtag gctttgggtg
      661 tttcgccgtg gctttcgccg acgaagatga cggacgcgtc gggcgactgc ttcagcagcg
      721 ggaacagggc gcgggtcagc cccataggtg cgacggtgtt gatgcggtat tggttgaccc
      781 attcggcgac ggtttggaaa tccagcggcg agagggcgta aaaatagccg gcgcagtgga
      841 cgatgccgtc cagtttgcct tgcgtggctt cggcaatggt ggcggcgaac tgattgaatt
      901 ctttttcttc cgcactcatc aggtcgaaac ggatggcgaa gggttcgggg tgtccggctt
      961 cgacaatcgc gtcatagact ttttccagtt ttttctgatg gcgtgccacc agaatcacgg
     1021 ttgcgcctgc cgccgcatag gctttggcga cctgttcgcc cagaccttgc gatgcgccgg
     1081 taactaagat ggttttgttg gacagtgtcg ccatactttt tcctttttgg tggtcggtcg
     1141 ggggtatttt agcgttttgc cgcaccttgt aaagcgtccc gataccagcc ggcgttttca
     1201 gacggcatcg ctcaggcttt gcaggcaggt ttccgccgcc gcgaaacctg attgtacggc
     1261 ggcttcgagc gtggcggggt agtccgggtg gaggtagtcg ccggcgggga agatgcggtg
     1321 ccggtgcagc cacgacaaat ccggcggcgg ggcatcggct gcggttgtgg cgcgtttttc
     1381 ggtgatgacg cgcacggctt cgggttcgcc caaatgcgga aggatgcgtt tgaggtcggc
     1441 gtgaactttg tccgcccacg ctcggtttgt aaacgcgccg acgcggtcgg aaacgctgat
     1501 gacggcggac acttcgtttt caggcagtcc gagcctgccc cggcaaagca gccattgcac
     1561 cgtgccgtcg gcaaggccgg tcagcggggc gggcaggcgg acgggttcgg cgtagcgcag
     1621 atagacggtg gtgatggcgt ggtagcgaag gttttgatat gccgtctgaa cgtgttcggg
     1681 cgtgccttcg ggcaggagcg cggcggcgtg gtagggcgcg gtggcgggga cggcggcatc
     1741 gaaagcttcg ccgttgacga gcactttccc gtccgggagg gtgttcagac ggcatacgcg
     1801 cgtttcgagg cggatgtccg cgccgagccg ttgaagatcc gccaaggcgg gttcggcgac
     1861 gattgcgccc aaatcctgct tgggtaggag atagtcgctg ccggattttt tcgtcagcac
     1921 gccgtcggac aaaacgttgc acaacacgcg caggcttgcg gtttccaaag gcgtgttgag
     1981 cgcgccccaa accaagggct gccaaaactg catcacggcg gcacgcggca cgttccgctg
     2041 tttcagccat tgcgccactg tcgtgtcggg ctgtccgagg cgtgcggact tctgcaaatc
     2101 ggacatatcg gcaagcagtt tggctttgaa tgcagtcggt gcacgccggg caagcagcac
     2161 gccgcccaaa atatgcagcg gcgcgggcag ggggagggcg cggaactgca aaccgccgtg
     2221 catatgccag tgcagcggta cgcgcaaaaa ggcggcacgg ggatccgaac cgatggtttt
     2281 catcaggcgc aacacgcccc ggtatgcgcc gagcaaaatg tgctgcccgt tgtccaaaaa
     2341 accgaaaccg tcggtatttc cggccagtgc gcgcgccctg ccgcccgcct gccggccggc
     2401 ttcaaacagg gtaacgtcgg cgtgccgcgc caaggtgacg gcggcggaca gtcctgccca
     2461 gcctgcgccg atgacggcga ttttcgggcg cggatgcggc gtgttcatca tttattcctc
     2521 caatggttga aaccccgttt ttcggggcgg cgggggttgg gttttcctga agggcgggcg
     2581 gtatgcgcct tatgcccgtt ccggcgtgcc ggggcgcggt ttgaatccga ataaccaggt
     2641 tttcagggca atgcgttttt tgcgcggcga agggagggcg attttgtatt tgaggacgtt
     2701 ttgcgcgccg tcccggtcga tttcgttcaa tagctcgtaa taaaccgccg ccataaccag
     2761 tccgactttt tgggcttttt tatcggcagc cggcagcagc gatacggctt cgcggtaggt
     2821 ttcacgggcg cgtttgattt ggaacgccat caattcggca aaattgcccg tcgggctgca
     2881 ttgcaaaatc acgcttgcgg ggacatcaaa ccgctgcatt tcctccatcg gcaggtaaat
     2941 ccgtcccctg cgcgcgtctt cgccgacatc gcggatgatg ttggtcagtt gcagcgcaag
     3001 tcccatcttg tcggcgtatt ccagcgtttt gccgtctgaa aaccccaaaa tccgcgcaat
     3061 caggcagccg actacacctg caacacggcg gcagtacagt ttcaattctt caaaactgcc
     3121 gtaacgcgcc cgaaccaaat ccatctgcat cccgtcgatt aaggcttcca gttcatattt
     3181 cggcagcttg aaggtttcct taacctgccg caaggcctga ttgacggggt gttccggcat
     3241 cgcgccgccg aataccttgt ccaaatcgcc gcgccaccag ttcaatgtcg cctgtgcaac
     3301 atcggggttg gaacattcgt caaccacatc gtccaattcg cggcaaaaag catataaaac
     3361 cgttaccgca tcccgttttt cctgagtcag gaaacggaag cccgacaaaa aactggagcg
     3421 gctttcttct gctttttggc ggcaatagtc gagtcctttc acgatttata ttcctaatga
     3481 tgggagggaa aggcggattt tatcggcatt tggcggtaga gggcaatttc ggcggcacga
     3541 cccaatcctt agcggtttgc tcaactatcg gcgcgaattc tgttaaaatg ccgacttcct
     3601 tcctttacac accgcaccga caggcagaat ttatggctct tttgcagatt tcagaaccgg
     3661 gtatgtccgc cgccccgcac cgacaccgtt tggcggcagg catcgatttg ggtacgacca
     3721 acagcttggt cgccaccgtc cgcagcggca gtgccgcctg tctgcccgat gccgaagggc
     3781 gcgttaccct gccttccgtc gtccgctatc tggaaaacgg cggcattgaa gtcggcaaaa
     3841 ccgccctgtc cgcccaaaaa accgacccgc tgaacaccgt cagctccgcc aaacgcctta
     3901 tcgggcggac gcttgccgat ctgcatcaaa atacgcatta cctgccttac cgtttcggtg
     3961 acaatcaacg cgttatcgaa ctgcatacgc tgcagggggt gaaaacgcct gtcgaagtgt
     4021 cggcggaaat cctcaaaacc cttaaatcgc gcgccgaaga aaccttgggc ggcgatttgg
     4081 tcggcgtggt gattaccgtt cccgcctatt tcgacgatgc gcaacgccag gccactaaag
     4141 atgccgcgcg tctggcgggt ttgaacgtat tgcgcctgct caacgaacct accgccgccg
     4201 caatcgccta cgggctggac aacgcctcgg aaggtacgtt tgtcgtgtac gacttgggcg
     4261 gcggtacatt cgacgtatcc gtattgcaac tgaccaaagg cctgtttgaa gtcaaagcca
     4321 ccggcggcaa cagcgcgttg ggcggcgacg atttcgacca ccggctgttc tgccgcctgc
     4381 tcgaacaaaa cggactctcc caactcaacg aacaagacag ccaactcctg ctctcgctcg
     4441 tccgcgccgc caaagaacaa ttaaccacgc aaaccgaagc gcgcattcag gcgacgcttt
     4501 cagacggcat ggcaatcgac acaagcatca gtcgcgccga gttccacaac ctgacgcagc
     4561 atttggtgat gaaaacgctc gagccggtca aacaggcgtt gaaagatgcc ggtgtcggta
     4621 aaaacgaagt caaaggcgtg attatggtcg gcggttcgac ccgtatgctg cacgttcaac
     4681 aggcagtcgc caccttcttc ggacaaaccc cgctgaacaa cctcaatccc gacgaagtcg
     4741 tcgcgctcgg cgccgccata caggcaaacg tcctcgcagg caacaaaacc gacggcgaat
     4801 ggctgctgct ggacgttaca cctttgtcgc tcggtttgga aacctacggc ggcttggcgg
     4861 aaaaaatcat cccgcgcaat tccaccatcc ccaccgcgcg cgcgcaggac tttaccacct
     4921 tcaaagacgg tcagaccgcg atgacgatac acgtcgtaca aggcgaacgc gaactggttg
     4981 ccgactgccg cagccttgcc aaattcactc tgcgcggcat tccgcctatg gcggcgggtg
     5041 cggcgcgtat ccgcgtaacc ttccaaatcg atgcggacgg gctgctgtcc gtttccgccc
     5101 aagagcaaag caccggcgta cacgcgcaaa tcgaagtcaa accttcctac ggcttggacg
     5161 acgacaccat cacccaaatg ctcaaagaca gcatgagcaa tgccgccgaa gatatggcgg
     5221 cacgggcccg tgccgaagcc gtggtcgaag ccgaaagcct gaccgatgcc gtcaacgccg
     5281 ccctcgagtt ggacagcgat ttgctggatg ccgaagaatt gcaacagatt cggcaaggca
     5341 tcgccgattt gcaaggccgt ctgaaagacg gaaaagccga agacatccgt gccgccgccg
     5401 ccaaactcgg cagtattacc gacaatttcg ccgccaaacg catgaaccgc aacatccaac
     5461 gcgcgttgac aggccagagt gtcgataata tttgatactt aaacggtttc agacggcata
     5521 gatataacct gatgccgtct gaaggctcga aaacacttga aaaacatcga tatggaaacg
     5581 tcaggcattg tctattcgat gaaaaccgtc atcaagggcg tgtatagtga attaaattta
     5641 aaccggtaca gcgttggctc gccttagctc aaagagaacg attccctaag gtgctgaagc
     5701 accaagtgaa tcggttccgt actatttgta ctgtctgcgg ctcgccgcct tgttctgatt
     5761 tttgttaatt cactatataa aatcggcatt tcggaggtaa gcaattttga aggcagaatg
     5821 cgccatttgg aaaacaacgg ctatgcgaac gttgccggat tggaacgcat cctcgccgtc
     5881 aaaaccgaca attacaaaga aaaagaaaac ctgctccatg aaattttcag caaaagcagg
     5941 ataggcgata ccgaattgtt cgccgtggac gaaaaccttg taaaacgttt gtttttatcg
     6001 cttcgcggcg aaatcgtgtt tccgaaaaac gaaacggcgg aatcggaatt tgaaaaaagc
     6061 gtccacgaac gcaggcagga agggaatgcc gggtccggcc gcaaacaact gcttgatttg
     6121 gtacggcgcg gacaccggga atacccttac gcgctgcccc ggcttttggc gggcgcggca
     6181 tcctacaagc cgaaaaaatc gaaaatccgc ctttttaaag aagcatattt cggcaaaagc
     6241 ggcacgaggc tgaccgacga aattgcagac ggcatccata tttacacctg tttttcgcgg
     6301 gcggatttgg aaaaagccta ttccgaatat ttggaacttt tcaaatccga atcggatgcc
     6361 gaaggcagaa agccgcagta aggtgcaaac agataccgta cacgttgagg agcagatatg
     6421 atgggcgatt ccgtcattta ttatgtagaa caggcagaca aaccggtaaa ccgtgccggc
     6481 gaacgcgccc gtaaaacatt caaatatttt tggcgcgagc ttttttggga acgccgccgc
     6541 atcattcccg ccttagattt tgccatggtc aaagcccctt ttttccaaga cggcgaagac
     6601 ggcgaaattt gcgaacatat gtggatcgac gatatttatt ttgacggtct ttacatttat
     6661 ggcgtgctga acaatgaacc cggcgaactg accaatgtcg aacaaggcga aagcgtttgc
     6721 gttccggttg acgacatcag cgactggatg ttcgtgtgca acggcattcc ctacggcggc
     6781 tttaccatac aggcaatgcg tggacagatg acggaagagg agcgtaccga acacgatgcc
     6841 gcatggggaa tcgatttcgg cgatcccggg caggtattgc tggtgtatga agaaaaagaa
     6901 catcccgaaa atctggaaga gcatccgatg tgccggaact gtattgacga ttttcggcaa
     6961 cagttgtccc aaaatccgga ttttctgcat gagcaggacg aagacggcta tacgccgctt
     7021 catcatgaag ccatcgcagg aaatgcactt atggttcaag ccatgcttga atacggcgca
     7081 aatcctgcct caaagacatc ggaaggctat accgccctcg attttgcccg cctggcgggc
     7141 tggcaaaatg ttgccgacct gctcgaaccg cgacattagg cggacagttt tccgaaaacg
     7201 aacacaaaca ctttttacag aaagacaata aaaatgccca aaatcaccgt acttccacac
     7261 acgacattat gccccgaagg cgcggttatc gaagacgcgc ccgaaggcaa aaccgtcctt
     7321 gatgtgctgc tcgaccatga tatcgaagtc gatcacgcct gcgaaaaatc ctgcgcctgc
     7381 acaacctgcc acgtgattat ccgcaaaggt ttcgacagcc tggaagagcc gaccgaattg
     7441 gaagaagacc tgctcgatca ggcttggggt ttggaagccg attcgcgcct gagttgtcag
     7501 gcggttgtcg ccggcgagga tttgattgtg gaaatcccca aatacaccat caaccacgcg
     7561 cgagaagaac actgaaaaca ggccgtctga agccggcacg cttcagacgg cattgttgcg
     7621 cggataaggc gcaatcgccc gaaaacaggc gttcgtacag gcggaacttt cgattctata
     7681 gtgaattaac aaaaatcagg acaaggcggc gagccgcaga cagtaccaat agtacggcaa
     7741 ggcgaggcaa cgctgtaccg gtttaaattt aattcactat atattgattt ttatcggttt
     7801 tctgacggaa cggattggtg cggcatccga ggcggattac tcgggcgcga tgcacgggta
     7861 tttatcggtt ttgcagccgg aaaaaccgcc ggcgggttac tttcagggcg gcggtcgggc
     7921 ggtatgccgg atagcgttcc actttcgctt ctatattgat tttgattggt tttctgacgg
     7981 aatgatccga tgcggcatcc gggacggctt gtgtttcttc ctgcccgcct gccggatatt
     8041 cctgtacttg cacgaaaccg aaagagacgg cggcgcggcg gacaagctcg agatagcgtc
     8101 cttcaagctc caggcaggcg gcggacacgc tttctaccgt aaccgtgaaa ccgccgcccg
     8161 acccggcaag gcgttccgca atctgcgtgt agatgagcca acgttgggcg cgcaacattg
     8221 ccgcatcgca accggcagcc gctttatcca aggcaatcag gccgcgccgg ttttggtggt
     8281 gaaggcaaac cgttactctg gagaggatat gttgcccgtc caatatttga tacagtttgc
     8341 gtatcagcag aatcaggcgg tcaaactcct ccatctccga cagggaatca ggatggtcgg
     8401 aagcggtata aaccagtttg gacaattttg cggcaaacat attgttcatc aatcttcctt
     8461 gtcggttgaa accccgctct tcggggcggt agaatcagat ttgtttggga gggacaactc
     8521 ctcccagatc aggacgacac ataggctggt gcttgatgtg ttgtccggcg agttgaaaca
     8581 ttcagccatc ctcaaggtgc ggcagttttg ccgaaacata ttctacacgg cttcaatgcc
     8641 ggacgataaa aggaaattca tatgaaatgg accgacaccc ggcgtatcgc cgaagaactc
     8701 tatgacctgc acggcgaaac catcgatccc agaaccgtgc gctttaccca actgcgcgac
     8761 ctgattatgg cattgcccga atttgatgac gatcccgccc gttgcggcga acgtattctc
     8821 gaagccgtgc agcaggcatg gatagacgag gcagaataag tttcgggaat gccgtctgaa
     8881 atacggcggt acgcggttcg tgcttctgtt tgcagtggga acggttttac cagtctcctt
     8941 ttttcagcct atccagttgg cggcggtcgc gcttggtcgg tctgccgtcg ggatgggcgg
     9001 aagtgatgcg gctgaattgg tcgagctgtt tgcgctcttc cctcaatgct gccgtttttg
     9061 cgtcttcttc atacagaagc cgcgcctcgg atgccgggcg gcgttggtgg ttcaaacctt
     9121 taaccttgat tttataggga agggaattga gcgtcaggtc gataatatcg ccgatgtcta
     9181 tggttttact gtttttgacc ttcgagccgt ttacttgaac cctacccagt tcgatgtgct
     9241 tttgcgcaag ggaacgggtc ttgaaaaaac gtgccgccca aagccatttg tccagccgca
     9301 tggcggaaga atcgtgcttg tctttcatac gattttgttt gaaataattg aatttgtttc
     9361 gagtttagca taagatacgc cgcctataac tagtatatat gcactaatcc actgttttcc
     9421 atgctgtccg aacacaaaaa gagggtgtgg aaaagccgtt ttggacaata aattaactgc
     9481 ggaatatgca caaatagcgt atgatagcgg cagaatctgt tgatgagagc ttcattctat
     9541 gaaacctgtt tttttggatt ttgaacaacc catagccgaa ctgaccaaca aaatcgatga
     9601 gctgcgtttc gtccaagacg agtctgccgt cgatatttcg gacgaaatac accgtttgca
     9661 gaaaaaaagc aacgacctga ccaaatcgat tttcagcaaa ctcacacccg cccaaatttc
     9721 acaggtttcc cggcatccgc agcgtcccta tactttggat tacattgagg cactgtttac
     9781 cgattttgaa gaactgcacg gcgaccgcca ctttgccgac gattatgcga ttgtcggcgg
     9841 attggcgcgt ttcaacggac aaagcgtgat ggtcgtcggg catcagaaag ggcgcgacac
     9901 caaagaaaaa atccgccgca acttcggtat gccccgtcct gaaggctacc gcaaagccct
     9961 gcgcctgatg aagacggcag aaaaattcgg cttgcccgtc atgaccttta tcgatacgcc
    10021 gggcgcgtat cccggcatcg gcgcggaaga acgcggacag tcggaagcca tcggcaaaaa
    10081 cctgtacgaa ctgacgcgcc tgcacgtccc cgttttgtgt accgtcatcg gcgaaggggg
    10141 ctcgggcggt gcgttggcgg tcgccgtagg cgattacgtc aatatgctgc aatattcgac
    10201 ctattctgtt atctcccccg aaggctgcgc ttcgattttg tggaaaaccg ccgaaaaggc
    10261 ggcggatgcg gctcaggctt tgggcattac tgctgaccgc ctgcaaaagc tggacttggt
    10321 cgataccgtc atcaaagaac cattgggcgg cgcgcatcgg gatttcgggc aaaccatgaa
    10381 aaacgtaaaa gccgttttgg aaaaacaact gcacgaagcg caaagcatcc cgcttgccga
    10441 tttgctttcg cgccgtttcg accgcattat ggcttacggc aaattttcgg aacaataatt
    10501 caggtagaac aagcagcaag cagtttgtct gaaactgctt gctttttctt tatcgggacg
    10561 gaaccgtgct gactttagat gcgtttgagc aatgcttgaa ggattgtttt cctcaaggtc
    10621 tgaatggaaa aaaaacagcg gtggcattaa gcggcggctt ggattccgtc gttttgctgc
    10681 atctgcttgt ccgcgccggg aaaaaaggcg gttttattcc ggatgcattg catatccatc
    10741 acggcttgag tccccgtgcc gacgattggg cggatttctg ccaaaactat tgcgatatgc
    10801 tcggggtggg gctggaaacg gttaagatct gcgtggaaaa aaacggtttg ggcatcgagg
    10861 cggcggcgcg gcaaaagcgt tatgccgcgt ttgccgaaaa aggctttgac gttttggcgt
    10921 tggcgcacca cagggacgat caaatcgaaa cctttatgct ggcggtcgcg cgcggcggcg
    10981 gtttgcgcgc tttggcggct atgcccgccg tccgcccttt tggggaaaaa ggcatcattt
    11041 ggcggccctt gctgcctttt tcgcgccaag atatatggga ttatgcccaa aaacacggtt
    11101 tgccgaatat cgaggacgaa agcaatagcg atacggctta tttgcgaaac cgcttccggc
    11161 accgtatttt gcccgaactt tcggcgcaga ttccccattt cgggcggcat ctgttgaaca
    11221 atgtccgtgc cttgcaggag gatttggctt tgttggacga ggtcgtcgtt caagactgcc
    11281 gttgggtttg cggagccggt tatttcgata cggcgcggtg gctgacgttt tccccgcgcc
    11341 ggaaaaccca tattttgcgg cattttttga aggaaaacgg cattcccgtg ccgaatcaga
    11401 atgcccttgc cgacattgcc cgggttttga cggaggcaaa aaccggacgt tggaacgtgc
    11461 aaggctttga attgcatcat tatgcaggca ggctgtttgt gttccgaccg gaaaaaacgg
    11521 ataaactgcg gtttttgaaa gacaggcaga taagcggaaa tttaagggaa atattggcgg
    11581 ggcagggatt tgttttaaaa cgtcatccgt tcggacttcc cgggcatctt ttggagcagg
    11641 acggaatctt gcggacggtt gcggcatcgg acaggctggc tgtcggcggt atccataagg
    11701 atgtgaagaa aatccttcag gaaaagcggg ttttgcccgc gctgcgcccg ttttggccgg
    11761 tcgtcgccga caaggacaac cgtccgctgg cgttggcaaa ttacggtacg gattttcaaa
    11821 tctcggtttc agacggcatt ttgcccgtcc atccggattt tcccgtttta ttttgatgga
    11881 atcggaatcg ccgttaccta ttatatattc ttgaaaaggc ggtaaagtga agcatatcag
    11941 ttcggcaaac aatgaacaca tcagacacct gcaccgcctg ttgtcgcaag ggaagttcag
    12001 acggcaatac gcccaaaccg ttttggaggg cgtgcacctg cttcaggttt tcctgcaatc
    12061 cggcggaagg ccagtcgggg tatatattcc cgaagcgaaa atgccgtctg aagaagtccg
    12121 taaattgacg gcggttttgc cggaagacgg ggttttttcc gtttcagacg gcatattgaa
    12181 gaaaatcagc agcctgactt gcgcggatga tgtgcttgcg ctgattgata ttccggatgc
    12241 gggtgctttg ccggccggcg gcgactgtgt ggttttggac ggcgtgcaag atccgggcaa
    12301 tgtcggcacg gtgttgcgga gcgcggcggc ggcaggaatc ggcgcggtcg ttttgggcaa
    12361 aggttgtgcg gacgcgtggt cgcccaaagt gctgcgagcc ggaatgggcg cgcatttctt
    12421 gtcggagatt tatccgcagg cggatttgga aatatggttg gcgcgttatg aagaccgtgt
    12481 gtttgccacc gcgttgcgtg aggaaaaaca ggcggttctg tataacgaag atttgcgcga
    12541 accgacagcc tgggtgtttg gcaacgaagg cgcgggggtc ggtaaagcag ttttagacag
    12601 ggcggacaag tgtgtcagga taccgatgca cgatgcaacc gagtctttaa atgtcgcgat
    12661 ggcggcgaca atctgcctgt ttgaacaaat gcgccaacgg gcggcgtatt gaggaaaaga
    12721 aatgccgtct gaaaaaatct attacggcgt attgattttc ttatgtatcg cttctatgct
    12781 gctgtcgccg tttttttatg cgggtgcttt gaagcccaag aaggcggcat tgcggaagga
    12841 cgggcagtgg aaactcatct tattgtccaa taccgtggcg gcggcggttt tggcttgggt
    12901 gtggtggaaa tggttttgac agatattgct caaaatcgtg ctaatggaat ccgaacaaat
    12961 aaagaatttg gtaaaaaatt tgttaaatca acgaattaaa gttttgtgga aaacaaaaca
    13021 gctctaagca aatagggcgt ttgtcggtaa atacggaaga gttgcggcat tatcgggcat
    13081 ctttaacaag tagtgccgtc ttgacaggca atcggttttt atgggcagct tgcaaaatcg
    13141 cggatataaa attgcaaatc ggttaaagtg tggggacgct atgaaaaatt gcgaattttt
    13201 ttatgacccg acaagggcaa tctatgatag cggtgcagat tatttaacca gagaaaaaca
    13261 tagattcgtc gtaattgcaa atagtgcttg ggggctattg cttaatttat cttgttatta
    13321 tgacgaggtt ttggaaaagc ggaaaatacc gttcggcaaa caggaaattg atgacgatat
    13381 ggacaaagtg tccgcccata agcggaagtt taaagatatt tctgaaatca aagtagggga
    13441 tggttgggaa tacccgttca attatgagca gggaatgaaa gaattagatg aagtgctatt
    13501 gaaatacatt cccttttttg aagaagaacg ataaaggaga ttgatatgcg cgtatctaaa
    13561 ataattggaa gtatgttgct tgttacagcg gttcagaccg tattttcggc aaatgtttac
    13621 gcgtgccgcc ataatggtaa aaccagttac agccaaactc cgggaaaaca ttgtaccaac
    13681 gcgggtttgg ggcgggatcg ggtgtacagt tcggttagac cggcagtaaa agacagggcg
    13741 gaagacgcag gggtcggcga ttattcggac acggtgaggg acgaacacgt ccaaaatccg
    13801 agagagaata cgcataagga tgcttcgaat accggtgcaa agacgcattg attgaaaccg
    13861 aatcagccat gacggcgttg aataatagaa tttgaacgat tagggaactc tgatgaaaca
    13921 catccacatt atcggtatcg gcggcacgtt tatgggtggg attgccgcca ttgccaaaga
    13981 agcagggttt gaagtcagcg gttgcgatgc gaagatgtat ccgccgatga gcacccagct
    14041 cgaagccttg ggcataggcg tacacgaagg cttcgacgcg gcgcagttgg aggaatttaa
    14101 agccgacgtt tacgtcatcg gcaatgtcgc caagcgcggg atggatgtgg ttgaggcgat
    14161 tttgaaccgc gggctgcctt atatttccgg tccgcaatgg ctggctgaaa acgtgctgca
    14221 ccatcattgg gtactcggtg tggcggggac gcacggtaaa acgaccaccg cgtccatgct
    14281 cgcatgggtt ttggaatatg ccggactcgc gccgggcttc ctcatcggcg gcgtaccgga
    14341 aaacttcagc gtttccgccc gcctgccgca aacgccgcgc caagacccga acagccaatc
    14401 tccgtttttc gttatcgaag ccgacgaata tgacaccgcg tttttcgaca aacgctccaa
    14461 attcgtgcat taccgtccgc gtaccgccgt gttgaacaat ctagaattcg accacgccga
    14521 catctttgcc gatttgggcg cgatacagac ccagttccac cacctcgtgc gcaccgtgcc
    14581 gtctgaaggc ttaatcatct gtaacggaca gcagcaaagc ttgcaggata ctttggacaa
    14641 aggctgctgg acgccggtgg aaaaattcgg caccgaacac ggctggcagg tcggcgaagt
    14701 caatgccgac ggctcgttcg acgtgttgct tgacggcaaa acggccggac gcgtcaaatg
    14761 ggatttgatg ggcaggcaca accgcatgaa cgcgctcgcc gtcattgccg ccgcgcgtca
    14821 tgtcggtgtc gatattcaga ccgcctgcga agccttgggc gcgtttaaaa acgtcaaacg
    14881 ccgcatggaa atcaaaggca cggcaaacgg tatcaccgtt tacgacgact tcgcccacca
    14941 tccgaccgct atcgaaacca cgattcaagg tttgcgccag cgcgtcggcg gcgcgcgcat
    15001 cctcgccgtc ctcgaaccgc gttccaatac gatgaagctg ggtacgatga aagccgccct
    15061 gcccgcaagc ctcaaagaag ccgaccaagt gttctgctac gccggcggcg cggactggga
    15121 cgttgccgaa gccctcgcgc ctttgggcgg caggctgcac gtcggcaaag acttcgatgc
    15181 cttcgttgcc gaaatcgtga aaaacgccga agcaggcgac catattttgg tgatgagcaa
    15241 cggcggtttc ggcggaatac acaccaaact gctggacgct ttgagata
//
LOCUS       DAXIEB010000042        15286 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000042 DAXIEB010000000
VERSION     DAXIEB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.042"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(84..3161)
                     /locus_tag="ACFOA3_001060"
     CDS             complement(84..3161)
                     /locus_tag="ACFOA3_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6898081.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDLGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIKADQDHYTAKMQGHKLMPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPR
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVSYNKSRYKNAAEVNAERLAKN
                     TGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYGLIDG
                     FVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            3435..4214
                     /locus_tag="ACFOA3_001061"
     CDS             3435..4214
                     /locus_tag="ACFOA3_001061"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6898082.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            4227..4367
                     /locus_tag="ACFOA3_001062"
     CDS             4227..4367
                     /locus_tag="ACFOA3_001062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898083.1"
                     /translation="MSDAFGWTVKPTRHENRVGKNAVWKNSDGIFGHITCKRQHIPTQ
                     TD"
     gene            complement(4425..5810)
                     /locus_tag="ACFOA3_001063"
     CDS             complement(4425..5810)
                     /locus_tag="ACFOA3_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6898084.1"
                     /translation="MIVASRWPLLEKAFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGSLHAYK
                     IKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFA
                     NKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGFWYFDKDLLDAFKAKF
                     KTDRKNIKEIG"
     gene            5928..6004
                     /locus_tag="ACFOA3_001064"
     tRNA            5928..6004
                     /locus_tag="ACFOA3_001064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5962..5964,aa:Arg,seq:acg)
     gene            6022..6096
                     /locus_tag="ACFOA3_001065"
     tRNA            6022..6096
                     /locus_tag="ACFOA3_001065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6055..6057,aa:Glu,seq:ttc)
     gene            6655..7830
                     /locus_tag="ACFOA3_001066"
     CDS             6655..7830
                     /locus_tag="ACFOA3_001066"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6898085.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8041..8589)
                     /locus_tag="ACFOA3_001067"
     CDS             complement(8041..8589)
                     /locus_tag="ACFOA3_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6898086.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDVAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(8592..10088)
                     /gene="ccoG"
                     /locus_tag="ACFOA3_001068"
     CDS             complement(8592..10088)
                     /gene="ccoG"
                     /locus_tag="ACFOA3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6898087.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLAVVIAAFLVGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10618..12597
                     /gene="tkt"
                     /locus_tag="ACFOA3_001069"
     CDS             10618..12597
                     /gene="tkt"
                     /locus_tag="ACFOA3_001069"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6898088.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFILSNGHASMLLYSLLHLTGYNLGIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            12742..14130
                     /gene="fumC"
                     /locus_tag="ACFOA3_001070"
     CDS             12742..14130
                     /gene="fumC"
                     /locus_tag="ACFOA3_001070"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6898089.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14195..15118)
                     /locus_tag="ACFOA3_001071"
     CDS             complement(14195..15118)
                     /locus_tag="ACFOA3_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6898090.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         3844 a         4375 c         3535 g         3532 t
ORIGIN      
        1 cgccccgata gacctgcctc cataaaaatg ccgtctgaaa cataccctgt ttcagacggc
       61 atccgcaaaa ctacggtttt caattaaaac tgccaatcca gtttcatgct gacagtgcgc
      121 ggctctccgt agaagttgtt tgcgccgcgc gtacggttgt agttgttctc aaaataagtg
      181 cgtccgttta agttcgtacc gatgaggctc aatttggcgt gtttgcccaa ttcgtaacgg
      241 acgaaaccgt ctatcagccc gtagccgccc tgacggatgt tgtacaggct gcttgtgccg
      301 ctttgtgcgg acacgccgcc gccgacggtc agccccgtat tcggtatatg gaagctcgtt
      361 ccgaaacgga atatgtgcac gggtgtgaaa ttgctgaagt tgtacgggtc tgcgcctgtg
      421 tttttggcga ggcgttcggc gttgacttcg gcggcgtttt tgtagcggct cttgttgtag
      481 gagactttcc aatcttcgtt caactcaccc gacaactcga attccgcacc cctgctgacc
      541 actttgccta tcggtttggc aacggtttgg aacgacccct gcttgccgcc tgctctggga
      601 acatagccaa aatcgacgac cgtgcggttt ttctgttcga ggtaaaacaa tgcgaacgaa
      661 gcattcagcc gtccttgcaa gaacgcgcct ttccagccta cctcatagtt tgtgccgacc
      721 aaaggcggta aaacggtttt ggcactgaca tcgacattat cctgctgttt gaagattttg
      781 gtataacttc cgtaaatact ctgttgcggt gtcaaatcat aggtaatgcc tgcatagggc
      841 atcaatttat gaccttgcat cttggccgta taatggtcct gatccgcctt aatgctcgat
      901 gccgtctgaa aatcgcttgc cggctgccca tagcggacag gcatatcttt ggtttgcgag
      961 gtttcatagc gcgtgtagtg cagcccgccc aaaaggtgca gtcggccggt tacgttgaaa
     1021 cgcgtgctgg cagtcagcga atgggttttg ttggtgttga ggtatttggc gtagttatac
     1081 agcgcaggaa catggtcatc ggccacttta acagttttcc aaaccggcac cgtaccggaa
     1141 aaaccggtaa agacaggcga gccgtcggga ttggtctcct gaatcttgtt gcctttttcg
     1201 tccagctcat atacatcgac atataccggt gtccggctgc cgctgtattc gtcatagtaa
     1261 tacacctgct tgccttcggc atcgagcttg ggctcggttt ttattttctt ggcattcctg
     1321 cattcttcgg cataaacggt acggttgcct ttttcatcgt acgcctgcca atcgggttct
     1381 ttatgccccc tgaccaaagg agaagacaaa tcaccgtccg gctcctcctg acaacttccc
     1441 gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg ttcgtagatt
     1501 tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa gaaatcatgc
     1561 tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca ttggcggtcg
     1621 tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc cttccgatta
     1681 aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa gatgccttcc
     1741 ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccag atccccccaa
     1801 gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc aaaaccggct
     1861 ttgcgttctg cggcacggcg gctgcgatac tgttcgaaag cagtattatc gaaacggttt
     1921 ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg atatgctttc
     1981 agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt ttcggtcaaa
     2041 aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag aaaaaactgc
     2101 cccaccttcg catcggattc attcttggta taagacactt cggcattgag ctgccaaccg
     2161 ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact caaccgcgac
     2221 caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca ggcaggcgtt
     2281 gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga aaaatcagga
     2341 acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc gcccgcatcg
     2401 gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt aaacgatttt
     2461 ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt caggctgcct
     2521 gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc tccggtatgt
     2581 ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg ctctgaattg
     2641 gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac cgccaaatcg
     2701 gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac attgacggta
     2761 ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg cgtctgcaag
     2821 cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc aagcgtatgc
     2881 accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg cgtatccttg
     2941 cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga acgggtcttc
     3001 tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccag actgacggta
     3061 ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc cgataacagc
     3121 ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc tgtaatatgc
     3181 aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat taattgaaaa
     3241 aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac aaaaaacaaa
     3301 ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata tccgaattta
     3361 aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg acaaacacac
     3421 tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa aagattttgt
     3481 gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc gaatcctacc
     3541 gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc tttatttggc
     3601 gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt gccgtactca
     3661 tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac accatcctgc
     3721 tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc aaggatttgg
     3781 tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa caacctgaaa
     3841 tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc gaagaattga
     3901 ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa tacggacact
     3961 cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta agcggaaaac
     4021 gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc aagttcaccc
     4081 cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat actttccaaa
     4141 cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa atcatgatgg
     4201 aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg gtcaaaccga
     4261 cacggcacga aaaccgcgtc ggcaaaaatg ccgtctggaa aaattcagac ggcatcttcg
     4321 gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca atcaatacga
     4381 acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc gatttcctta
     4441 atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccagtaaatc tttatcgaaa
     4501 taccaaaacc cgtcatccat ttccaatgcg ccgcccattc catgcagaac aactttttcc
     4561 cccgttaaag gatcggtttc ggtaatttcc acctcatgca tttttcccca atccaaaaca
     4621 ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccacacgc cttatcgcat
     4681 cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg aaggataccg
     4741 gctgcggcag gacacaatcc gtatgtttcc gcccacaacg acaaagcccg tctcgctgcc
     4801 tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt caaagccgct
     4861 gttttcgcat tcaacatgcc gtctgaacct ttttcaaacc tgacggtcgt aaatgtttta
     4921 agcagatttt tggcagatac ataacaaccc gaatgataac gtccgaccaa ttccgcttta
     4981 atcttatatg catgcaggct gcccaatgca ggaaaaaaac ggacttcctc cgtattgcac
     5041 caatcaaacg gggcttttcc ggaatccaat aaagccgaaa cctcgctata tacccgttca
     5101 aacgtaccga tgtaatttac ttttcctccg ccgtcgaaac aggccagcac ccccataccg
     5161 tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg cagcggtttc
     5221 ggattcatca aacggaaaca ctgcctgatc catgcctcaa ccccgtgttc cgacagactg
     5281 tattccaaat aatcacacaa tgccgataca tccgccatcg cacgatgcct gtcttccaca
     5341 acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg cggatacaga
     5401 caccgggaca gctgcacact gcacaaagca ggcgatgaaa atccgatacc cgcacgatga
     5461 aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag cacacaaccc
     5521 ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc ggcaaccatg
     5581 ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg aggattaacc
     5641 agccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac ttcggttacc
     5701 ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac cggcattcca
     5761 aaccgtaaaa acgccttttc cagcaagggc caacgggaag caacaatcat tttattctct
     5821 ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc aacaaaacaa
     5881 ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgcagtgca cccgtagctc
     5941 agttggatag agtatctggc tacgaaccag agggtcgggc gttcgaatcg ctccgggtgc
     6001 gccagtaaga aaatacaata tgcgcccatc gtctagcggt taggacatcg ccctttcacg
     6061 gcggtaaccg gggttcgatt ccccgtgggc gtgccaaatt ccaaatcccc gagattatcc
     6121 ctcggggatt ttttattgcc tcggcaactc gttaccatat ctttacctac ccccttcatc
     6181 agaatctcag acgtaatcga atcatattca aacctttgcc gtgcaaaccg atatcccata
     6241 accggatgcg gtgtccgtcc aacattttac ccgattgaaa cgcctgatat attgcacccc
     6301 atcaacgtgg cattactttt cttaacaatc ccctttgaca gcaactgact agggcttttt
     6361 tatgccatca tcaaatttat agtggattaa ctttaaacca gtacggcgtt gcctcgcctt
     6421 gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     6481 ataatatttt ctctcccgat tgaaacaggc gtaacagaat atgcccgaag ctccggctgc
     6541 tttcttgttt accgccgcga tatttagagt ataataccaa atttgagcaa tggttctaaa
     6601 acagttagaa ccatttttca tgagcctgac tgattcgtac actcggagaa actgatgcag
     6661 aatatttttg accctttggt tattcgtgga aaatccctta cccccatcgt gcaaggcggt
     6721 atgggggtcg gtgtttccgc atcgggtttg tccagcgcgg tggcacgtga aaacggtatc
     6781 ggaacgattg ccagtgtgga tttgcgccac cttcacgaag acctactcgc cgaatcccaa
     6841 atcaatccga gtgaagagaa atatacgtct ttgaactgta ccgcattgga cagggaaatc
     6901 caaaaagcca aaagtgcttc agagggaaaa gggctgattg cggtcaacgt gatgaaggcg
     6961 gtcaaagacc acgccgcata tgtccgccag gcttgcgaat caggggcgga tgcggtcgtg
     7021 atgggcgcag gcctgccttt ggacctgccg gaaatgaccg agggctatca taaagatgtc
     7081 gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata tcgtcttgaa acgttggatg
     7141 aaaaaaggca tattgcccga tgcgattgta gtcgaacatc ctgcccacgc ggccgggcat
     7201 ttgggcgcgt caaccgtcga aggcgtaaac gatgctaaat tcgacttcaa acgcgtgatt
     7261 gaggaaacgt ttgaagtttt caaaagttta gggctggaaa gcgaaaaaat cccgcttatt
     7321 ttggcaggcg gtatggcaaa ttttgaaaaa gtcaaaaccg ccctaaagaa ctggggagca
     7381 tccgccgttc aaatcggtac ggcttttgcc gttaccgaag aaggagatgc acaccttaac
     7441 ttcaaaaaaa cgctcgccgg tgcggaaact gaaaaagtag ttgaatttat gtctgttgcc
     7501 ggtttgccgg cgcgcggcgt acgtaccaaa ttcctagaca gctatatcag gcgcgaaggc
     7561 aaacttcagg caaacgccaa agccgacccg cgccgctgca cccaaggatt aaactgcctg
     7621 accagctgcg gcctgcgcga cgggctttcc aaagccgggc aattctgtat tgatatccag
     7681 cttgctgccg cattccgtgg agaagtggat aaaggcctgt tcttcagagg taaagacccg
     7741 ctgcccttcg gcaatgccat ccgcaccgtc cgcgagacga tacaatatct gctgacgggg
     7801 agcgaacctg ttgcaacgct cggacgctga catcaggtag gatttgattt gcaaacaaaa
     7861 tgccgtctga agggccttca gacggcgttt ttcaggctgc gttctggaat agtgttaaaa
     7921 aataaacggg atgagctgca tttattttcg tccgacaaat caaaccatca cgccgaatga
     7981 tcaaataatg cctgcacggc gttgcatctg gcaaatcaat gcaatgaaaa cacggctttt
     8041 ttatttgctt tcagtattat tgaaaagttt gtccatcggg gtcaaatcga ccgcattgcc
     8101 ttggctggta atccatttgt tctcgacgcg ccacacgcct gccacgtcct ccacgcgcac
     8161 ataccagtgg ttggtcggcg aaagggtttt gaacaccgcc tcatattccg ccctgccgtt
     8221 ctgcgcgctg ccgacgggct tgagggcgac agtttggtcg tccgccttgc gggtcgggtg
     8281 catcagcagc aggttcaaag gctgtttgcc gtcaaactcg ccgccgacaa acacttttgc
     8341 cgcattcata tcgggggaaa tgagaacctg caccccgata tgccgtctga cggcttcttc
     8401 atcccgatga agctggatgt cgatatgttt gccgtcttta taataatcgt ccgtaaccaa
     8461 atctgtcgcg tgctgctgcg cgacaaaaaa catagcgacg ctggcgatga cgacaaaaat
     8521 cggccccgcc atcaagatcc acggccagac gtgtttgtac caaggtttga ttggagtgtt
     8581 ttgagacacg gttattctcc gataaaggtt gcatcttctt ccaagacgac cggcttgccg
     8641 tcgggcgcgc cgctttcgcg gtattggaag gtaaattcga tagggtggct gcctttgtcc
     8701 gcgtattccg gaatggtgga cacttggacg ggaagggtta ccgtttcgcg cggggcaacc
     8761 ttgataccgc cttcgggcag cccggtcagg gcgatttcgt caaagccttt gacacttgcg
     8821 gtaatgagct gttctttttc acttttgttg atgatacgca ggctgtatgc gttttccagc
     8881 cagcctttgg cgttttcgcg caccagtacg ccacggtctt tcaaaatatc gacctcgacc
     8941 attttgcgcg tggacaaacc gaccaaaaag gcggcgataa ccactgccaa caccgcgccg
     9001 taacctgcca cgcgcggtct gagcagccgt tttttaatgt ctttttcaga atattcgtgt
     9061 tccaacgcgc tttcggtcgt ataacggatt aatccgcgcg gatagcccat tttgtccata
     9121 atctcatcac acgcgtcgat acaggcggcg cagccgatac attggtactg cagaccgttg
     9181 cggatgtcga tgccgacagg gcagacttgg acgcacatcg cacagttgat gcagtcgccc
     9241 aaacccgcct cttccttatt gaccgttttc ttgcgcgcgc cgcgcggttc gccgcgttcc
     9301 gcgtcataag aaacaatcag cgtgtccttg tcgaacatcg cgctttggaa acgtgcatac
     9361 ggacacatat gcaggcatac tttttcgcgc ataatgtggg cgaagaagaa ggtcataaag
     9421 ccgtaaaacg ctgtggcaaa catcgcgccg ccgcctgctg ctccagtgaa taaatcggga
     9481 acgaactggc ggatagggac aaaccagcct gcaaacgtga tgcccgtcca cgcgcagaca
     9541 aggaaaatca acaggtattt ggtggctttg atgcggattt tagtgaaatt ccacggcgat
     9601 ttttccagtt tcagccgttt gtttctatcg ccttcgacca ggttgtcgat ccacagcata
     9661 atttcggtgt aaaccgtttg cgggcaggaa tagccgcacc acagtcgccc ggcaatcgtc
     9721 gtccaccaaa acagcccgaa ggcgcaaatc atcagcagca aggcaaggta aatcaaatcg
     9781 cccaccccca acgacaatcc gaaaatgaag aaatgccgtt cggggatatt gaaaacgaca
     9841 gcctgcctgc cgctccagtt gaaccacgga atgacgtaaa acacaaactg cgtcgccaat
     9901 acggcggcga tacgcagttt ggcgaaccgt ccttccgcct ttttcggatg gatgcgttcg
     9961 ccttcgggat ggatttgaat cacgctggct cgcggatcga acgttttttt tgctttcggc
    10021 gcggcttttg tttgttcgga cgtgccgatt ccggatgccg gactgccggc ttggttttcc
    10081 gtggtcattc tgcattcctt agatttttga ttgatggttt gccctttacc gccgccgttt
    10141 gcttttcaga cgtcattttt cttgtttttt aaggcgttgt gtttcaagtt ttgagaaaat
    10201 ccgtttttcc caaaatatat ttccgctatt gtacaacttt atgcgccgtc cggatgtatg
    10261 gggcggatac atttcccatc cgcatcaaaa cgcctggatt ttaccttacc gcccgaacaa
    10321 aatccgaata cggttaaaaa aaaagactaa aaaaaaaccg acacccccat atcggcagaa
    10381 ccgacggcgc aagctcataa acaaacgcta tcgacaatcc ggcacacaat ctataacttt
    10441 ttatttcaaa aggaataatg gcaggcttcg cccgcaaatc gaaaatcctt ccccaccggt
    10501 ctcctgccgc cgccttcccc cgcgtccgcc cttttcttga aagcataagc gaatcgggcg
    10561 ataatcaacg ccttccgatt atccactcat ccgaaacacc agcaaggaaa atacaaaatg
    10621 tctcaactgg caaacgcaat ccgcttcctc tcggcagatg ccgttcaaaa agccaattcc
    10681 ggccaccccg gcgcgcctat gggtatggcg gaaatggcgg aaacattgtg gacgaaattc
    10741 ctcaatcaca accccgccaa ccccaaattc tataaccgcg accgcttcat cctctccaac
    10801 ggccacgcgt ctatgctgtt gtacagcctg ctgcacctga ccggctacaa tctgggcatt
    10861 gaagacctga aaaacttccg ccagctgcac agcaaaaccc ccggccaccc cgaatacggc
    10921 tacaccgacg gcgtggaaac cacgaccggc ccgttggggc aagggattgc caacgcggtg
    10981 ggtatggcat tggcagaaaa aatccttgcc gccgaattta ataaagacgg tttgaacatc
    11041 gtcgatcatt acacctacgt ctttatgggc gacggctgtc tgatggaagg cgtatcgcac
    11101 gaagcctgtt cgctcgccgg caccttgggc ttgggcaaac tgattgtttt gtatgatgac
    11161 aacaatattt ccattgatgg taaagtggac ggctggttta ccgaaaacat cccgcaacgc
    11221 tttgaaagct acggctggca cgtcgttcct aacgtaaacg gtcatgacac cgccgccatt
    11281 caagccgcca tcgaagccgc acgtgccgaa accggcaaac cgtccatcat ctgctgtaaa
    11341 accttaatcg gcaaaggcag tgccaataaa gaaggcagcc acaaaaccca cggcgcacct
    11401 ttgggcgcgg acgaaatcga agccacgcgc aaacatttgg gctggactta ccctgccttt
    11461 gaaatcccgc aagaaattta cgatgcgtgg aatgccaaag aaaaaggcgc gaaactggaa
    11521 gccgaatggg acaaactgtt cgcgcaatat caagccaaat atcctgccga agccgcagaa
    11581 tttgtgcgcc gtatggataa aaaactgccg gaaaactttg acgagtacgt tcagacggca
    11641 ttgaaagaag tgtgcgccaa agccgaaacc atcgctaccc gcaaagccag ccaaaacagc
    11701 atcgaaatct tggcaaaaga gctgcccgaa ctggtgggcg gttctgccga cctgaccccg
    11761 tccaacctga ccgactggtc aaacagcgtt tccgttaccc gcgacaaagg cggcaactac
    11821 atccactacg gcgtgcgcga gttcggtatg ggcgcgatta tgaacggttt ggcattgcac
    11881 ggcggcgtaa aacccttcgg cgcgactttc ctgatgttca gcgaatacga gcgcaacgcc
    11941 ctgcgtatgg cggcactgat gaaaatcaac cctgtatttg tgttcaccca cgattccatc
    12001 ggtttgggcg aagacggccc gacccaccag ccgattgagc aaaccgccac cctgcgcctg
    12061 attccgaata tggacgtatg gcggccgtgc gacaccgccg aatccttggt cgcttgggca
    12121 gaagccgtca aagccgccga tcacccgtcc tgcctgattt tcagccgtca aaacctgaaa
    12181 ttccaagcgc gcagcgaaca acaactggac gacatcaaac gcggcggcta catcatcagc
    12241 gaagcccaag gcaacgccca agccgtcatc attgccaccg gctcagaagt cgagctggct
    12301 ttggaagcgc aaaaagccct cgccgcgcaa aacatcgccg tgcgcgtcgt ttccatgccg
    12361 tccaccaacg tattcgaccg ccaagacgcc gcctatcaag ccgccgtcct gcccgaaagc
    12421 ctgccgcgca tcgccgtaga agccggacac gccgacggct ggtacaaata cgtcggtctg
    12481 aacggcgcag tcgtcggcat caaccgcttc ggcgaatccg cccctgccga tttactcttc
    12541 aaagcattcg gctttaccgt ggacaatgtg gtcgatacag tgaaatccgt gctgtaaacc
    12601 gcagccgccg catcaaatgc cgtctgaacg cttttcagac ggcatttttt ttgccctcca
    12661 gccttaatgc ttcctcattc cgccaatctc cattataata tttgcgaatc actcttattc
    12721 acatttcaaa aggagaaacg catgagcacc cgtaccgaac acgacaccat gggcaatgtc
    12781 gaagtcccgt ccgaagccta ttggggcgcg cagacccagc gcagccgcaa caatttcaaa
    12841 atcggcggcg aaaccctgcc gcagccgctg atttatgctt tggcgctggt gaaaaaagcc
    12901 gccgccgcga ccaatgtttc cctcggcagg attaagccgg aacaggcgga tttgattacg
    12961 caggcggcgg atgatgtgtt gaacggcagg cttgacggac agtttccttt agtggtttgg
    13021 cagaccggct ccggcacgca gtccaatatg aacatgaacg aagtgctggc aaaccgcgcc
    13081 aacgaaatcg ccggtacggg tttggcggct tatcagcccg tccatcccaa cgaccatgtg
    13141 aaccacgcgc aatcgaccaa cgacgcattc ccgaccgcta tccacgttgc cgccgcgatt
    13201 gaaatcaacc gccacctcat ccccgccgta aaagccctgc gcgatacctt ggacaaaaaa
    13261 gcccaagctt tcgcccccat tgttaaaatc ggccgcaccc acttgcaaga cgcgacgccg
    13321 ctgactttgg gacaggaatt ttccggctac gtttcccagc ttgatcacgg cttaggccgt
    13381 ctgaacgatg cgcttaaaga cttgtatgaa cttgctttgg gcggtacggc ggtcggcacg
    13441 ggtttgaaca gccatcccga atacgccgaa aaagccgccg ccaaactcgc cgaactgtcc
    13501 ggcttgccgt ttgtcagcgc gccgaacaaa tttgaagcct taggcggacg cgatgccgcc
    13561 gttgccgctt cgggcgcatt gaaaacgctg gcggcaagcc tgaacaaaat cgccaacgac
    13621 atccgctggc tggcaagcgg cccgcgttgc ggtttgggcg aaatcaaaat ccccgaaaac
    13681 gagccgggtt cgtccatcat gccgggcaaa gtcaacccga cccaatgcga agcaatgacg
    13741 atggtgtgct gccaagtgtt cggcaacgac gttaccatcg gtatggcggg cgcgtcgggc
    13801 aatttcgagc tgaacgtcta tatgcccgtt atcgcctaca acctcttgca atccatccgc
    13861 ctgttgggcg acgcgtgcaa cagcttcaac gaacactgcg ccgtcggcat cgaacccgta
    13921 ccggaaaaaa tcgactattt cctgcaccat tccctgatgc tcgttaccgc gttgaaccgc
    13981 aaaatcggtt acgaaaacgc cgccaaagtc gccaaaaccg cctacaaaaa caacaaatcg
    14041 ttgcgcgaaa ccgccgttga gttgggcttg ctgacgggcg aagaatttga cgaactggtc
    14101 gttcctgccg atatggttca tccgcgctaa tctttccctc aaataaaatg ccgtctgaaa
    14161 cctcgttcgg acggcatttt ccgttgcctg caaactagcg gcgtttgaac agcctgtccc
    14221 ccaccgccgc tgtaaccgca cccccgacca cgaccagtgc gccggcataa cccaaaccgt
    14281 tcatatccgg cgcggcaaaa gtatcaggca tcacataatg cccgagcaaa gaaaatatta
    14341 cggtaaacac ggggagcaag gttgttaccg cgctgacttt ggaagcctcc caatgtttca
    14401 acgcctcgcc gaacgagccg taaccgatta acgtattcaa gcagcaatac gcaaaacaaa
    14461 cccacgccaa cgtaccgtcc aaacttccga tgtgtgccgg ttcggcaaac ggcaggaaca
    14521 cggcggcact tgccgcataa atcaacagca gaatctgttg cggcccgaat tgcgccgaca
    14581 gcagcttttg cgccacggca taacacaccc atgccatact gcctgccgca cacagcaaca
    14641 cgcccttcgc atacgcgccc aaacccgaca actcgccgaa tttatcgtta aaaaacataa
    14701 gcaaaccggc aagcagcaaa accaagccga ttttctgagc ggcagtcatc cggtctttaa
    14761 acaccaacac accgacaaca atcatcgtaa acggcgaaat ctgccacaaa acctgcgtcg
    14821 tggtcggcga aatataatgc agcccttggg caatcaacac aaagtttgcc gaaatgcccg
    14881 ccacgccgag cagcagcagc ctgaatgagc accaagaaaa atcccgccgc ttcggcagcc
    14941 gcccgcccaa tgccagcaaa acaaacaata ccgccgccgc cacggtaaaa cgcacccaca
    15001 ccagcgtcgg cgcatcgaca aacttcaata cctgccgcac ggcaatcggc agcgttcccc
    15061 acgtcatcgc cgccaaaagt gccaacgcga agcctaggag cggcctttgg ttttccatcc
    15121 tgattttcct attttttaaa caaccgtatt gccggacgat gccggtttgc cgcatcgggc
    15181 aatgatggtt caagcgtttg gcgtttgatt ccaacccttt gatttcaaac aaaccggctg
    15241 aagcccggct attgcttcgc gctatttgaa aacaccgcct gaattt
//
LOCUS       DAXIEB010000043        15090 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000043 DAXIEB010000000
VERSION     DAXIEB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.043"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(3..323)
                     /locus_tag="ACFOA3_001072"
     CDS             complement(3..323)
                     /locus_tag="ACFOA3_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6898091.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
     gene            517..1243
                     /locus_tag="ACFOA3_001073"
                     /pseudo
     CDS             517..1243
                     /locus_tag="ACFOA3_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            1312..2850
                     /gene="lnt"
                     /locus_tag="ACFOA3_001074"
     CDS             1312..2850
                     /gene="lnt"
                     /locus_tag="ACFOA3_001074"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6898092.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDSNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3114..3977
                     /gene="rpoH"
                     /locus_tag="ACFOA3_001075"
     CDS             3114..3977
                     /gene="rpoH"
                     /locus_tag="ACFOA3_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6898093.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4036..4182)
                     /locus_tag="ACFOA3_001076"
     CDS             complement(4036..4182)
                     /locus_tag="ACFOA3_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898094.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
     gene            4461..5345
                     /locus_tag="ACFOA3_001077"
     CDS             4461..5345
                     /locus_tag="ACFOA3_001077"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6898095.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5429..5944)
                     /locus_tag="ACFOA3_001078"
     CDS             complement(5429..5944)
                     /locus_tag="ACFOA3_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6898096.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6019..6438)
                     /locus_tag="ACFOA3_001079"
     CDS             complement(6019..6438)
                     /locus_tag="ACFOA3_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898097.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6559..7557)
                     /gene="holA"
                     /locus_tag="ACFOA3_001080"
     CDS             complement(6559..7557)
                     /gene="holA"
                     /locus_tag="ACFOA3_001080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6898098.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEINKLGLLYPKGHTVNIDQAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGEKQTLAPIAVRRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7559..8038)
                     /gene="lptE"
                     /locus_tag="ACFOA3_001081"
     CDS             complement(7559..8038)
                     /gene="lptE"
                     /locus_tag="ACFOA3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6898099.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8090..8869)
                     /gene="pgeF"
                     /locus_tag="ACFOA3_001082"
     CDS             complement(8090..8869)
                     /gene="pgeF"
                     /locus_tag="ACFOA3_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6898100.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVLRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9022..9246
                     /locus_tag="ACFOA3_001083"
     CDS             9022..9246
                     /locus_tag="ACFOA3_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898101.1"
                     /translation="MMPLYTFSELYMFQQGMAHQAPHCVLPERDYPPIRFYRYKQMGF
                     NRKGMGIKSISDISRAMPSENQSPLSDGIV"
     gene            9323..10270
                     /locus_tag="ACFOA3_001084"
     CDS             9323..10270
                     /locus_tag="ACFOA3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6898102.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10423..11547)
                     /gene="rluD"
                     /locus_tag="ACFOA3_001085"
     CDS             complement(10423..11547)
                     /gene="rluD"
                     /locus_tag="ACFOA3_001085"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6898103.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11546..12349
                     /locus_tag="ACFOA3_001086"
     CDS             11546..12349
                     /locus_tag="ACFOA3_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6898104.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12424..14643)
                     /locus_tag="ACFOA3_001087"
     CDS             complement(12424..14643)
                     /locus_tag="ACFOA3_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6898105.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     SGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3637 a         4562 c         3687 g         3204 t
ORIGIN      
        1 cactatatcg accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg
       61 gtgtcggact gcatttctat tcggctatac gccttttcac gcaggctata attgaatttc
      121 cacaaaccgc cgaacactgc cagcggacgc gcgaccagca tcagaaacgc cgcaatcgcc
      181 aaggcttccg ccgccccgtc caacacgcct gcgggagaaa ccagcagcaa aggcattccc
      241 aaacgtgcgg acaaagtggt cgaaaccacg ctcaggaaca acagtgcgcc acctggcagg
      301 aacagactgc ccatccaatc cacgctaacg actctccctt aaaattcatt aaattaaaat
      361 cgaaagactg taacacggca tttttttcac gaaactttac ttcacgtccg ccaccgcctt
      421 cagacggcat tttcctgttt catgccgcct gaaaccgccc aaatccgcgt tctcctatat
      481 aatgtcggac tttgcgccgc atttcaggat tctttaatgg catttgcctc actatttacc
      541 ctgccggacg acatcaccgc cgttttggac gatgttgccc tgatgaccaa agccgccgca
      601 aagaaaaccg ccggcgtggt cggcgacgat ttgacaccca atgccaaccg ggttacgggc
      661 gtatcggcag aacgcgagtt gccgattgtt tggtcgatgg cgaaaggttc tttttccaac
      721 aagccggttt tggtgcctgc gcccctgctg ctttccgcct tcctgccgca actcattacg
      781 ccgcttttga cggcaggcgg catttatctg tgctttgaag gcgtagaaaa gctgctgcac
      841 aagtttctgc accgacatga cgcgcatgct cgtaatggcg gcaatcggca tcggcatgac
      901 tgttttggtg tacggaatcg ttgccgtcat cgtcaaactc gacgacttag gcatgctgct
      961 gatgcgccgc ccacaaacct tttcgcgcag tttggggcaa atgctgactg cctttatgcc
     1021 ttgctttatg cgcggtttga gcgtggtcgg cacgcttgcc atgtttctta tcggcggcgt
     1081 actggtcgcc cacaacctcg gccttctgca cgatttcctg cacgcgcaac actgggacgc
     1141 tggctgggcg gagtacttcg ccaacctcgt tgtcgggctg ctttccggtt cgattgcctg
     1201 cgcccccgcc ttgccgctga tgaatcgttt cggcaggcat tgatttcttt ttcaaatacc
     1261 gatgccgttt gaaagatgtt cagacggtat cttccgaaca gacagatgaa tatggtttcc
     1321 aaactggaca aatactggca gcaccccgcc ctctactggc ctttgctcat cctttttgcc
     1381 gccgccaccc cctttacctt cgcaccctac taccactttt ggctgatgcc cttgattttc
     1441 ggtgccttcg tccgcctcat cgaactgcgt ccgcgttttg ctgtctcttc cgcctacctg
     1501 ttcggcctga ccgcatacac gacacagttc tactggatac acaccgccct gcacgacgtt
     1561 tccggcctgc ccgacctcta tgccgtaccg ctgaccttcc tactccccgc ctaccttgcc
     1621 ctttatccgg cactgtgttt ctggctgtgg aaaaaattta ccctgcctcg gggcataaaa
     1681 atcggtttgg tactgcccat cctgtggaca ctgaccgagt ttgcccgcga acgtttcctg
     1741 accggattcg gctggggcgc aatcggctac tcccaaatca ccccggacag cccgctcgcc
     1801 ggctttgccc cattgggcgg catccacatg gttacactgg caaccgcctt tctcggtgtc
     1861 tggctggttt tggcgagtga taacaccgca cgttcgggca aacgcctgct tccaattatc
     1921 ctgattgccg ccctgcttgc cgcaggctac accgcccgac aaaccgactt cacccgcccc
     1981 gacggcagcc gcagcaccgt cgccctgctt caaggcaaca tcgaccaaac cctcaaatgg
     2041 cgtgaagacc aagtcatccc gaccatacag aaatattacg aacaggtcgg caaaaccact
     2101 gccgacatcg tcatcctgcc cgaaaccgcc atccccgtta tgcgccaaaa cctgccggaa
     2161 aacatactgg caaaatttgc cgaacaggcg caaaacaacg gcagcgcgct cgccgtcggc
     2221 atcagccaat acacttcgga cagcaacggt tacgaaaacg ccgtcatcaa cctgaccggt
     2281 tatcaggaaa acaatcagga cggcatcccc tactacgcca aaaaccacct cgtccccttc
     2341 ggcgaataca aaccgctgcc cttcctgacc acgccgcttt acaaaatgat gaatatgccc
     2401 ctttccgact tccgcaaagg cggcggtaag caatccgccc tgctgatgaa aaaccaaaaa
     2461 atcgccttca acatctgtta cgaagacgga ttcggcgacg aactgattgc cgccgccaaa
     2521 gatgccacac tgcttgccaa tgccagcaat atggcgtggt acggaaaatc caacgccatg
     2581 taccagcacc tccaacaatc gcaggcgcgg gctatggaac tcggacgcta tatggtccgt
     2641 gccaccaaca ccggcgcaac cgccatcatc tcccccaaag gcaacatcat cgcccaagcc
     2701 caacccgata cggaaaccgt attggaagga cacatcaaag gctatgtcgg cgaaacgccc
     2761 tatatgaaaa ccggcagttc atggtggttg atgggcatat tgaccctagc cgcactgatt
     2821 cttttcatct tccgaaacaa agaacactga tacctgcaca gcccgtccgg acgggctgtc
     2881 ttttttcaaa acggctattt acatacaatt acgtatgtaa tataattctg aaatcgaatt
     2941 gagccatcag gtttacaaaa tcaatcaaac ctgccttaca aaattttcct gtctttcaaa
     3001 ccttagcgca tatctcctgc caaacataca aaatacaagc caagcagtga tatactgcat
     3061 cccaacacag caaaccgccg atacgcagtt tcagccgaaa ggaaatgccc caaatgaata
     3121 acgctttcgc attacccgcc atccaaagcg gcaacggcag cctcgaacaa tacatccaca
     3181 ccgtcaacag catcccgatg ctgtcccagg aagaagaaac ccgcctcgcc gaacgccgga
     3241 taaagggcga cctcaacgcc gccaaacaac tcatcctgtc ccacctgcgc gtcgtcgttt
     3301 ccatcgcgcg cggctatgac ggctacgggc tgaatcaggc agacctgatt caggaaggca
     3361 atatcggact gatgaaggcg gtcaaacgct acgagccagg tcgaggtgca cgcctgtttt
     3421 catttgccgt gcattggatt aaagcggaaa ttcacgaatt catcctgagg aactggcgcc
     3481 ttgtccgcgt tgccaccacc aaaccgcaac gcaagctgtt tttcaattta cgcagtatgc
     3541 gtaaaaacct caacgccctg tctcccaaag aggcacaaga catcgccgac gatttgggtg
     3601 tcaaactttc cgaagttctg gaaatggaac agcgcatgac gggacacgac atcgccatca
     3661 tggcagacaa cagcgacgac gaggacagtt tcgcccccat cgactggctt gccgaccacg
     3721 attccgaacc gagccgacaa ctatcgaaac aggcgcatta cgccctgcaa acagaaggtt
     3781 tacaaaacgc tttggcgcaa ctggatgaca ggagccgccg tattgtggaa agccgctggc
     3841 ttcaagacga cggcgggctg acgctgcacc agcttgctgc agaatacggc gtctctgccg
     3901 agcgcatccg ccaaatcgaa gcaaaagcca tgcagaaact gcgtggtttc ctgaccgaag
     3961 aagccgaagc ggtttgaaat atacgcaata aaatacccga caaattgttt tgtcgggtat
     4021 tttatttggt aaacatcaat gcggtttgtg cgtcgtgccg tacacgattt tatccgtcca
     4081 aaccatcgca atcgccgaaa tcaaaaagca gacatgcacc agacactgcc gcatcagctg
     4141 cttttccgac agattggcgg aattgatata gtggattaac aaaaatcagg acaaggcggc
     4201 gaagccgcag acagtacaaa tcgtacggaa ccgattcact tggtgcttca acaccttaga
     4261 gaatcgttct ctttgagcta aggcgaggca acgccgtact ggttttggtt aatccactat
     4321 acatttccct tccttgcagc aacaaaagcg cgtgattcca atctgccgct gcaaaatctt
     4381 caatttttca cacccgaaaa cgcttgccgc catacagatg tgatatattg attccatttt
     4441 aacgggcttc ggagaacgac atgagcagcg gaaatatcca catccacagc atgaccggct
     4501 ttgccaacgc ggcggcggaa tgcggcagca aacgcatcaa cctcgacatc cgcgccgtca
     4561 accaccgctt tttggacatt caaatcagga tgccggatga tttgcgccat ttggaaagcg
     4621 gtatccgcga gaaaatttca tcccatctgg cacgcggcaa agtcgaatgc aaaatccaaa
     4681 ttcaagatac ggaaaacggc aatcagtctt tggaactgaa tcgcgatctg gtcggacagc
     4741 ttgccgaaat caacaaagac ctccgcaagc atcacgattt ggcaaaactg ggcgttgccg
     4801 acatcctgcg tttccccggc gtattggcaa gccaaaggga aaatacggac gcattggcaa
     4861 aggttattgc cgatttgacc gattcggcac tgaaagactt taccgccgca cgccgacgcg
     4921 aagggaaaaa attgggcgaa cacctgctgc aacgcctcga gtctatggaa gaaatcatcg
     4981 gggcattgag cgaaatcttc cccgcgctag tggaagcgca taaagaaaaa atccgcgccc
     5041 gccttgccga agccgtcggc gacatcaata atgacaggct gcaacaggaa tttgccctct
     5101 ttattcaaaa atccgacatc gacgaagaat tcagccgcct gcgcacccac attgccgaag
     5161 tgcgccgtat cgttaccgaa cacaaaggca gcagcggcaa acggctggac ttcctgatgc
     5221 aggaattaaa ccgcgaagcc aacactttgg gcagcaaatc cattgccgcc gaatgcaccc
     5281 aagcctcggt cgaactgaaa gtcttaatcg agcagatgcg ggaacaagtg cagaatatcg
     5341 aataatcggt ttccccgata agcaaatgcc gtctgaaagc cctttcagac ggcatttttt
     5401 caggttgaaa cctttgcggt ataaaggctt aaggctttcc tttgaccgcc accaatcttg
     5461 ttcctgcaag aaaatcatac agaaactgcc gatcgggatt cagcagtgcg aacccccacg
     5521 gtaaaatcag ccaaatcaat gccgcgccgg ccgcgccctt gggcggaatg ccgaggaagt
     5581 ggcgtaatcc ggcataggct aacataggga taaatacgat aaatatgcac gcccaaataa
     5641 agcgcaggcg cagcaggtgc aaagacggct gtatgccgtt aaggtcgcac aagccgattt
     5701 tccatgtcct catcgccaag gtctgcccct gaccatgcca gttggcgcgg aaataaagcc
     5761 accatgctcc cattatcagg atacttgtta ccaatgcaga aaccgcgata gaaacgggat
     5821 tcagaaaaat ggcggcaata ccggcaaaca atgctgccaa acaggttgcc gcaccgacca
     5881 gcagcatttc gtacagcatg gcggcaaagc ggcgtttgag cggggcggtt ttcagtatca
     5941 tcatcttttc aatcggaata tgccgtctga aacgccaaca ggcttcagac ggcattcatg
     6001 cagatatgtc tcatgtggtt aacggtgcgg acgtttctga ccacgcgact gttttgccgc
     6061 catttgattg atttcctgac gtgtcgggcg ttttttgaac atagcgtgcg cctcgtccac
     6121 cgaacatttt tgcgacaggc agatcagggt aatcgcatct ttctggtgtt gtccgccgct
     6181 gtggtacata cggcgcaggt ttgcctcgtt gattctgcct ttttcgggat cgatgtcgaa
     6241 ttttttcagg cgttcgataa actggcgttg ctgtcggcgg gatgtccaaa caaaaccgcc
     6301 cgcagcggca gcgacaatga tggcggcaag caggatggca agcggccagg aatcgacaat
     6361 caatacgata atgaggctta aaacggcggt ggtcagtaaa atcaggccgc ccaagcgcag
     6421 tttggtctta ttgtccatag ggttaatgtt cctttattga ggtggtcgtg ccgtctgaaa
     6481 tgttttcgga cggattggtg aatacttttc aggattaacg gtattttaca gtattttcgg
     6541 attttgggga ttaccgcttt attccgccag cgacacgaca agccgtttaa ataccgtcca
     6601 tgcgtcgccg tcttccgcac ctttgatgat tcggtcgatt tgggcgcagg ttttgagcgc
     6661 gtcaagcagg cggacgacgg aaatccgtct gaccgcaatc ggagcgagcg tctgcttttc
     6721 accccaaagc ctgaggctgt tgcggacgga ttggatgctc tgcccctgct tcagggcggc
     6781 ggcaagccgg atcagcgtcc gcacgtcttc ggcaaccgcc cacagcaaca gcaccggttc
     6841 ttcgccttcc tcctgcaatc cgtccaacaa tctgcatacg cgcgggacat cgcccttcat
     6901 ccacgcgcct gccagttgga acgcgtcaaa gcgggcgacg ttggcaacgg cggtttgcgc
     6961 ctgatcgata ttgacggtat gccctttcgg atacagcagc ccgagcttgt tgatttcctg
     7021 acgcgccgcc aacaaattgc cttccacgcg ctcggcaaac agtgccaatg cgtctgcctc
     7081 gatacccaaa ccgattttgt ccagccgtcc gcgtatccat tggggcaaag ccgccgcgcc
     7141 gaccggtttg gcttcccaca cttccccctt tgccgccaat gcggcaaacc atttggactg
     7201 gagctgggtt ttctccagtt tgggcagcaa aaccagcgtt accgtatctt ccggcaggcg
     7261 ggcggcaaaa tcctgcaacg cctcgccgcc ggttttgccg ggttttccgt tggggatatg
     7321 cagttccaac agcttcaaat cggcaaacag acccgcactg cctgcggttt gcaacagttc
     7381 gttccaatcg aaggcattgt ctgccgtata aacttcccga ttaaggtaac cttgtttctt
     7441 cgccgccgcc ctcaatgcgt ccaatgcctc gatacgcaac agttcttcct cgccgtggat
     7501 gacgtacaga ggtttcaaag gcgcgtccgt atcaatgcgt ccgatatgtg ccgccacgtc
     7561 attccgcctt cagaaaggtc aggcggcgga caatctgttc ggcggcatcc tgccgcattt
     7621 ccgcccacaa tgccgcttcc tcttcctgtt tgcccaagat ctcgttgtcg gcataagcaa
     7681 ggacgcggcg gacggacacg gtcatcggtt tgccgaccgg ctcgccgcgt ttcaatacct
     7741 gcgcttcaac cgtcaatatc aaaagatatt cgttgatgac tgccgcacgg gtaacggtgt
     7801 aggtttcctt gttttgggaa acgctgtcta tacgcagggt catctgcgcg ccggcagcat
     7861 cgtccaccct gcccgaagcc tgatacagcg cggtttccaa aggaaactgc aatgcctgtc
     7921 cgccttcgat gtgccagctc cggtaggtca gcggcggaga aatgccgtct gcacctttca
     7981 ggtggaaacc gcacgcgccc agcatcagca ctgcggcagt aagaaacagt ttgttcatgg
     8041 cttgcctttc tcaaaagcac aaagtcggta gattatatca gcggcgtgtt cagacggcat
     8101 tgccgtccag ccaaatcagg ctcgccatac gccctgtcgc tccgtcgcgg cggtaggaaa
     8161 agaaagtgtc ccgttccaaa accgtacaat gcgtgccgcc atatacgccg cccacgcctt
     8221 cacgcttcag aatcaggcgg gcgagcgcgt aaaggtcggc aaggaatttg ccgccgccta
     8281 tgccttcaaa tgcggcggcg gcttcgggca tgggcgtgca gaacgcatca aacacatcct
     8341 gtccgacttc aaacgcatcc gcactgatgg cagggccgag atacgccatc atttcgacgg
     8401 gcggaacctt catcgcggca atggtgtttt gcaatacgcc gcccgccaaa ccgcgccagc
     8461 ccgcgtgtgc ggcggcaacc gtcgtacccg ccctgtcgca aaacaaaaca ggcaggcagt
     8521 ctgcggtcat cacggcacag gcaaccttgc ccgtgtcgtc cacggaagcg tccgcatcgg
     8581 gcgtgccgtc caatgcttcg gcagcattga cgacaatggt gctgtggatt tgattgaggt
     8641 aggcaacggg caatccgacc tgctgttgca cgatttcgcg gttgcggagc acggcttcgg
     8701 gattgtcgcc gacgtgcgta ccgaggttca aactctgata cgcaccttgg ctcacgccgc
     8761 cgttgcgcgt ggtaatcagg gttttcacat tggcgggcgc gggccaatcg gcggtcagga
     8821 agtttttgcc tttcggggca agatttaggg tttctgtgat ggttttcatt ggatattgga
     8881 ataggtgtgt ggcggaaaag gttgattaac tgccttcggg gcaaatggat gcttgttttt
     8941 tcgtcattcc cgcacaggcg ggaattcgga gattcgggat tgttttcaaa cgttcgggtt
     9001 tggattcttg ccggtgcagg aatgatgccc ttatatactt tctccgagct ttatatgttt
     9061 caacagggga tggcacatca agcaccgcac tgcgtgttgc ccgaacgaga ctaccctccg
     9121 attagattct atcgctataa acagatgggt ttcaaccgaa aaggaatggg gataaagtcc
     9181 atttccgaca tctctcgggc gatgccgtct gaaaaccaat ctccactttc agacggcatt
     9241 gtttaggcgc ggtcattttg caaattaagg ttaaggcgtg tatgcttgcc ttcccttaaa
     9301 aacaaacacg caacgcatta ttatgaatat cctcagtaaa atcagcagct ttatcggaaa
     9361 aacattttcc ctctgggccg cgctctttgc cgccgccgct tttttcgcgc ccgacacttt
     9421 caaatgggcg gggccttata ttccttggct gttgggcatt attatgttcg gtatgggttt
     9481 gacgctcaaa ccttccgact tcgatatttt gttcaaacat cccaaagccg tcatcatcgg
     9541 cgtaatcgca caattcgcca ttatgccggc aaccgcctgg ctgctgtcca aactgttgaa
     9601 cctgcctgcc gaaatcgcgg tcggcgtgat tttggtcggc tgctgcccgg gcggtacggc
     9661 ttccaatgtg atggcctatc tggcgcgcgg caatgtggct ttgtcggttg ccgttacgtc
     9721 tgtttccacc ctgatttccc cattgctgac tcccgccatc ttcctgatgc ttgccggcga
     9781 aatgctggaa atccaagcgg ccggtatgtt gatgtccatc gtcaaaatgg ttttgctccc
     9841 cattgttttg ggtttgattg tccataaggt tttgggcagt aaaaccgaaa agctgaccga
     9901 tgcgctgccg ctggtttccg ttgccgccat cgtgctgatt atcggcgcgg ttgttggggc
     9961 aagcaaaggc aagattatgg aaagcggcct gctgattttt gcggttgtcg tactccacaa
    10021 cggcatcggc tacctgctcg gcttctttgc cgccaaatgg accggcctgc cttatgatgc
    10081 acaaaaaacg ctgaccatcg aagtcggtat gcaaaactcg ggcctggccg ccgcgcttgc
    10141 cgccgcacac tttgccgccg cgccggtcgt tgccgttccg ggcgcattgt tcagcgtgtg
    10201 gcacaatatc tccggctcgc tgctggcaac ttattgggca gccaaagccg gtaaacataa
    10261 aaaaccctaa acgcctttca tcataatgcc gtctgaacgg ggcaaacaaa accggcggtt
    10321 tctgcccggc cccttcagac ggcctttgtt tgaaatcggc ggggacgatt gcccggctgc
    10381 tccgattcgg ctgcctgcgt tcgccccgcc tttcaagccg cctcactccc ttacataaac
    10441 cacttcgaca tcatagtcgt cttcgttcca atcatcgtcg tcgtccgcgc cgaatttgtc
    10501 ctgccattct tcttcattgc tcaaagacga atccaaaccg gcttcaagac ggaggacaga
    10561 caacaaatga tatatgtcgt ccggaatcgg tgcctcaaac gaaacggttt cgccgctttc
    10621 cggatgggta aaactcaagc ggtaggcgtg caacgcctga cgcgcaccca aacttttaac
    10681 ggcttctttc accgtgtcgc cgcacggatg gcgcgggttg ccgtaaaccg ggtcggcggc
    10741 aagcggatgg ttggcctcgc gcatatggac gcggatttgg tgcgtcctgc ccgtttcgag
    10801 cgagcattcg atatagctgt gggtaagata gcgttccaac actttgacgt gggtaacggc
    10861 aggcttgccg ccgaatttga cgactgccat tttcaggcgg ttgtgcggat cgcgtccgat
    10921 ttgggtttcg attttaccgt caaaggggac gatgccgttg gcgacggcgc ggtagatacg
    10981 tttgaccgtg cgttcttgaa gctgcctcac gagggaattt tgcgccgaca gggttttggc
    11041 aaccaccatc agcccgctgg tttccttgtc caaacggtgt acgatgcctg cacgaggtac
    11101 ttggctcaat tcgggacagt gcgccaagag gccgttgagc agcgtccccg tccagttgcc
    11161 cgccgccgga tgcaccacca gtccggccgg tttgttgacg acgatgacgg tatcgtcttc
    11221 gtaaataata tccaaagcca taggctctgg aacaaacgcc agattttcct cactcggacg
    11281 gacggttaca caaatttgct cgccgcctat cattttgtct ttgggttgcg aaggtttatc
    11341 gtttacaata accgcgcctt ctttaatcca tgatgtcagg cggctgcgcg agtagtcggg
    11401 cagaagtttc gccaacaccg catccaaccg cccgccggca agctcgagcg gaacggtcag
    11461 accgatacgg ttttcagttt cggaagctga cgcaaagtct gaatcgtcgc tataatcgga
    11521 ttcattatca aaggaagtat tctgcatgaa aaaaattctt ttaacggttt cattaggttt
    11581 ggcactgagt gcctgtgcca ctcaaggtac ggtcgataaa gatgctcaga ttacccaaga
    11641 ttggagtgtg gagaagctct atgccgaagc ccaggacgaa ttgaacagca gcaattatac
    11701 gcgggctgtc aagttatacg aaatcttgga atcgcgcttc cccaccagcc gccatgcccg
    11761 gcaatcccaa ctggataccg catacgccta ttataaagac gatgaaaaag acaaggctct
    11821 ggcggcaatc gaacgcttcc gccgcctcca tccgcagcat ccgaatatgg attacgcgct
    11881 gtatctgcgc ggcttggtgc tgttcaacga agaccagtcc ttcttgaaca aactggcctc
    11941 gcaagactgg tccgaccgcg acccgaaagc caaccgcgaa gcgtatcagg cctttgcgga
    12001 actcgtccaa cgcttcccga acagcaaata cgccgccgat gcaaccgcac gcatggtcaa
    12061 actggtcgat gcactgggcg gcaatgaaat gtcggtggcg cgctactaca tgaaacgcgg
    12121 tgcatacatc gccgccgcca accgcgccca aaaaatcatc ggcagctatc aaaatacacg
    12181 ctatgtcgaa gaatcgctcg ccatattgga acttgcctac caaaaactcg gcaaaccaca
    12241 gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc ccgaaaagcc cgtttttgac
    12301 gcacgcttgg cagcccgacg atatgccctg gtggcgttac tggcactgat tccgcaccga
    12361 tgtcatgatg ccgtctgaaa ccctttcaga cggcattttc cgttccggca ccagcctttc
    12421 cgttcactca aacggttttt tctgccaata gaatttatac cctctcaaac gttgggcctt
    12481 cacgccgccg cgccccaagc cgaattgcag cgcgcccgac aaatcggtac gcagcagttt
    12541 gatgccgtgt gcgcggacac ggttctgcac cgcttcggtc ggatgtttgt aggcgttggc
    12601 ataaccgctt gaagcaacgg catattcggg cgagacggca ttgaggaaca cgcccgacga
    12661 ggacgtattg ctgccgtgat gtcccaaaac caaaacctgg ctgtacaggt tgcctccata
    12721 cttgccgacc aggctttcct cgcccttcgt atccaaatcg cccgttacca gcagtgccgc
    12781 accgcccgcc acaacacgca aaacacaact tttcccatta tcatcgatgt ttttgcgttc
    12841 agacggcatc aaaaactcaa aatccacgcc gtcccattgc caacgctgtt ccgcacaatg
    12901 ccgcgccccc tcataaaatt ccggctgtcc ggcataaatc ccgctgttcg gtatcttgcc
    12961 gactgcctga aaaccgccgt cgtggtcgct gtcgtgatgc gacagaacca gcttgtccaa
    13021 acggcggaca cccgccgcat tcaaactcgg cacaatcccc atctgcgccg ccgctgccgt
    13081 tccggtgtca aacaaaagat gatgatttgc cgtctgaacc gacaccgaca aaccctgccc
    13141 cgcatcccaa accgtaaccg caacctcatt ttccggcacg ccgggtgaac ggtaaaacac
    13201 aaaccctgcc aacagcaaca ccgcccacgg acgcaaaccc aagccgcgcg gcagcagcaa
    13261 cagcaaagcg gcacacaccg ccaacaccaa cagcggcaaa ggtgcggagg caacggcaaa
    13321 ctcgggcgac acatcggcaa gccacaccaa aaaccgcaaa gtatattccg ccaaaaatgc
    13381 ccccaactgt tgcaaaggcg caaacggcac gaccgaaccc agcaacgcca gcggcgtcaa
    13441 tacccaagaa aaccacggaa tcgccaccgc attgaccaaa gggctgatta aaggcagcga
    13501 agcaaacaga taaccgagca aaaccagcga caacaccgaa gccgcccact gcccgcgcac
    13561 ggcggtttgc cgtttccctt cgtgcaaacg ccccgaacac gcccatatca gggccgccac
    13621 caaaccgaaa gacagccaag tccccacacc caagaccgcc aaagggtcga acagcagcac
    13681 tgccgccaac gcctgccacc acgtcgccca cgccgacaat cttcccctgc gccaagccca
    13741 cgcaaacgcc gccaacatca aaacgctgcg ctgcgtcggc acggaaaaac cggcaagcag
    13801 cgcgtaaaac agcgcgcctg cacaccccgc cgccaaaacc cacacgcgcg gccgggcagg
    13861 aaggcgcggg gaacaggcaa gcagccgctt cgccagccac gcaaacatca ccgccaccat
    13921 cgtaacgtgc aaacccgaaa tactgaccaa atgcgtcagc cccaacggtc ggaacgcctg
    13981 ccacaattcg gggcgcaatg ccgactgttc gcccacgctc aacgcgcgca tcagcccgat
    14041 gccgtctgaa agcccgccgt ccgcatccgc ctgctgccaa ttacggctga tgcggctgcg
    14101 ccaaaccgca atcccccaac cgcttccgcc atgcagcaaa accctgtccg caccgaccgt
    14161 ccccgcgccg cctatcccgt tggataatgc ccacgcctca cggttcaaac cgcgcaggtt
    14221 caattcgccg acgacggggt gcacacgtgc cgttatccgc catctgctcc cgaccgccca
    14281 ttcgcgccgt ttgtagtccg acagcagcaa atcaaacgtc cgaccaccgc tgtccacagc
    14341 ctttgccgca aactgcacgc gccgcccgtc cgacctcggc atatccgcca cttccaccgt
    14401 caacggcaca cctgaaacgg cttctgccca ccattgggaa gacaatgccg cctccgtcct
    14461 gaatacgccg taagccgcgc ccgccaacac gcacagcatc agaccggcaa acgcaaaacg
    14521 ccttgcaaac acagcaaaaa ccgcaaaagc cgccagccaa aacagccaat gcggcacgac
    14581 cggcagcgcg aacgaagccg ccgcaccgac aacccaacac ggcaaaaacc aacgacggaa
    14641 cacgtctccc caccccagat atttcaaaaa taaacggata ataaagcaaa tccgaaaaac
    14701 cgtcataaca agatttgaaa atgatatagt ggtttaacaa aaatcaggac aaggcgagaa
    14761 gccgcagaca gtacaagcag tacgacaagg cgaggcaacg ctgtactggt ttaaatttaa
    14821 accactataa gaatgcaaac gaaaagcctc agtattatcc gagacaacgg caaactttcc
    14881 atcgtcattc ccacgaaccc acatcccgtc attaccacga aagcgggaat ctaggacgca
    14941 gggttaagaa aacctacatc ccgtcattcc ctcaaaaaca gaaaaccaaa aacagaaacc
    15001 taaaatcccg tcattcccac gaaagtggga atctaggtct gtcggtgcgg aaacttatcg
    15061 ggtaaaacgg tttctttaga ttccacgttc
//
LOCUS       DAXIEB010000044        15086 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000044 DAXIEB010000000
VERSION     DAXIEB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.044"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(148..843)
                     /locus_tag="ACFOA3_001088"
     CDS             complement(148..843)
                     /locus_tag="ACFOA3_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFC6898106.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1014..1673)
                     /locus_tag="ACFOA3_001089"
     CDS             complement(1014..1673)
                     /locus_tag="ACFOA3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6898107.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1876..3986)
                     /locus_tag="ACFOA3_001090"
                     /pseudo
     CDS             complement(1876..3986)
                     /locus_tag="ACFOA3_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4172..4777)
                     /locus_tag="ACFOA3_001091"
     CDS             complement(4172..4777)
                     /locus_tag="ACFOA3_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6898108.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4826..5224)
                     /locus_tag="ACFOA3_001092"
     CDS             complement(4826..5224)
                     /locus_tag="ACFOA3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6898109.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5332..6243
                     /locus_tag="ACFOA3_001093"
     CDS             5332..6243
                     /locus_tag="ACFOA3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6898110.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QATLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6233..7534
                     /locus_tag="ACFOA3_001094"
     CDS             6233..7534
                     /locus_tag="ACFOA3_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6898111.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(7602..7772)
                     /locus_tag="ACFOA3_001095"
     CDS             complement(7602..7772)
                     /locus_tag="ACFOA3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898112.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLMIESEKNGRY"
     gene            7929..10079
                     /gene="dinG"
                     /locus_tag="ACFOA3_001096"
     CDS             7929..10079
                     /gene="dinG"
                     /locus_tag="ACFOA3_001096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6898113.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            10116..10730
                     /locus_tag="ACFOA3_001097"
     CDS             10116..10730
                     /locus_tag="ACFOA3_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6898114.1"
                     /translation="MNFTRLLNQVLSTVQKKGGTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAKRRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(11021..12889)
                     /locus_tag="ACFOA3_001098"
     CDS             complement(11021..12889)
                     /locus_tag="ACFOA3_001098"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6898115.1"
                     /translation="MQTVRYRPEIDGLRAVAVLSVIIFHLNDRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSHGNALQ"
     gene            complement(12970..14340)
                     /gene="purB"
                     /locus_tag="ACFOA3_001099"
     CDS             complement(12970..14340)
                     /gene="purB"
                     /locus_tag="ACFOA3_001099"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6898116.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14412..15044)
                     /locus_tag="ACFOA3_001100"
     CDS             complement(14412..15044)
                     /locus_tag="ACFOA3_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6898117.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3601 a         4158 c         3890 g         3437 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccgct atatattctg atttaaaacc ataaggcttt
       61 aagcaatcat ctcttctata aagcctaaat acaaaaggcc gtctgaaatc ctattttcag
      121 atggccttta ctcattcaat cctcaactta ttgcggcttt ttctgctctt cgcgtacttt
      181 atccaccaac tggtcaatcg tggtcatacc ggactgccag tctttaaatt caacttggta
      241 tttgccgccg acaatcacag tcggtgtgcc gctgatttgg aatttattgg tcaactcttc
      301 catttgagcc gcacgcgctt ggctttcagg agcctcaaat gcagccaata cttttttgcc
      361 gtcaaacgct gtttgctcgg acagccattt tttcagggta tcggtatcgg ccagattgat
      421 tttttgatta accatcgcat cgaaaatatg gctgttggct ttatctgatt caccggccat
      481 ttccactgcg gccgccaaac gtgccaaagg tttcatttca tcaccccaca cgacatgctc
      541 ccggcgcata taggtatcgt ctttaaacgt tttgatgtgc tcgctcaaga ccggctcaag
      601 atgggcgcaa tgcgggcaga agtagccgaa aaattccaat acttcgattt taccggcctg
      661 ctgttgcgga ataggcgtag acaatacagt gtagttcaca ccttcgttca actcagcagg
      721 ggctgccgga gcagatgagc tgctttgggc gctgtctgcc ggaacactgg tttcagcctg
      781 tttgctacaa gcggccaatg ccaacagggt caatgaagtc aaagctaagg ttttcagttt
      841 cataggtatc tcttgtgtgt cagattaatg tgcggatttt atggcatttt attgaaggcg
      901 tgttcgattt gagtgaaaaa atgtgtttag ttttatttca gcctttataa aggccgtctg
      961 aaatatggct tagataatta gccttatttt cagacggcct ttgttctagt aatttacggt
     1021 ttcggacatt ttgtcttcaa tccgtcgatg tgttccatta agtcaggtgg gacatgattc
     1081 cacataggat caagaataaa atcgatacct tcccgtctgg ctaatttggc tgctgggaca
     1141 aaatcggaat caccagaaaa caagatgatt ttatcggctt gtttttttaa ggtaatagat
     1201 gaaatatcca agcctatacg catatctaca cctttttgtt taacatcgag cacgacatcg
     1261 ttttccgtaa tttgagttga cgggtttttc agaagatttt tcatgacttg gggttttaat
     1321 atccatgctg tattgtccga cagcttgcct aaacgtaacg ctaatttacg cttcttcttc
     1381 aattcggtat gcagattgca acgaaatatg gcttccggag tctttgaaaa atctatggca
     1441 cgatgagata cgggattgtg cattttcttt tcaaacggca cgcagtcgta aaagaaaatt
     1501 ctatataaat catccatttg atctgtgccg ttgattttat ctttcagatg cagcatcgaa
     1561 tatcggaaag ccaaatcagc aattcttttt gcatcgtgtg gattttgaga caggcctaat
     1621 gaccgtaccc gcttaataaa aaatgcaccg tcaatcaaaa tggcggtttt catattgctt
     1681 cccctatatt tgtcaaagat ataaaaaagc ccttgggatc ggcatacccg ctatgattta
     1741 atgggtaatg cgtactgcca agggcgctat ttctttttgg attatactga tgtagataaa
     1801 gcctgtcaaa aagttcgtgt gctgaattta cggattatca ggattttcag acggcattct
     1861 gttgcacgaa cgggattaga aactgtaatt aacccgcgcc gtataagtgc gcggcgcgcc
     1921 gggcatggca tccgaacgcc aatatttttg attgagcaga ttggctgcgg caaaggtaat
     1981 gttaacattt ttatggttcc agccaagcat ggcatcaact cgggcaaagc ctggaagcgt
     2041 agtcacttct ttatttcttg agttgtaacc gtagcgtttg cctgtaccgg ttacgccgat
     2101 ttcgccgtag aggttttcgg tcggggtata acggaaaaac aggttgccgg taacgttgct
     2161 ggtgttattc aaatggatgc ccactcggtc gggattttct ttgtcttcaa cgactttcgc
     2221 ctgaatcacg cccaacgaac cgcgcagata gagttttttg gggatgattt gcccgatggc
     2281 ggacaattcc acgccacgcg aacggtgttt gccgccgact gcccaagtgt agggattatt
     2341 ttgtgcgtcg gggcggtagc ggatattgaa gcgttcgatt tggtaggcgg acaatgtggt
     2401 gctgaggcgg tcgtccagcc aactgctttt aacgccggtt tcgtattggc gggtgtactc
     2461 ggggtcagcg ttgaacacgg cagaagatga cgtatcgatg ctcaaatagc cgccgcgtcc
     2521 gccataaggc gcgaaacctt tgttatacga ggcgtaaagt gtgtgaacgg ggttgatgtt
     2581 ccacactgcg ccgatgttgg ggctgaacga gtggccgctg tactggcggc tgctgccggt
     2641 gagtttgttt tcggaattaa aggtgtattt gtcgtaacgg ccgccgagga cgaatttcaa
     2701 atcgggcgtg gcggagaaga tgttttgcac aaagatgccg taggagtcgg ctttgtggcg
     2761 gttttgggtg aggataggct gcaatctgcc cgaagccggc cagcttgctc ggtcgtatgg
     2821 atcgatggaa gcagtaaagg cgcggctgta acctaaggtc gggttgcggt gttcgcggct
     2881 gtaatccatg cctacggtca agtggttttc aaaacggccg atggtgtagt cgccgtttag
     2941 cgtgaaattg gacgacaggg ttttgttgtc ggtctgctgc caggcgtagt tacgtttgat
     3001 taagttgcca ttttcgctgc ctgcatagaa atgatcaaaa tcctgcgccg ccgtgcggtg
     3061 ggcgagctgc cattgggcac gccatttgtc gttgaaggcg tatcaaggtc ggagcgccaa
     3121 acttgcagct tgtctttgac aaaatcgttc cggtgggcga accccatgcg gtaaggcagt
     3181 ccgaagcggt cgtacacgga cttggtcgga ctgcggtcgg gcgtgcgctc cacattgtcg
     3241 taggtgtatt gccccgtcca cttcaagccg ttgtcgagtt tgacggtaat gctgggcgaa
     3301 accatgacat ttttgctgtc tatgccgctg cggaacgaat tggcgcgccc gacttcgccg
     3361 gtgagacgga tggcgacgtt tttgttcaac acttcgttaa tgtccatatt caggctgcgg
     3421 tttgcccacg aaccgtaaac cgcaccgatg ttgcggcttt gtttgaagtt ggcgtatttg
     3481 ctgaccatgt tgatgacgcc gccgccgttg gtgcggccgt aaagcacgga agacgggcct
     3541 ttcaggattt ccacgcgctc gatgttggca gtactgcggc gcacttgtcc gctttcgcgc
     3601 acgccgtcgc ggtaaatatc ggatgcgtcg gcttgaaaac cgcgcaggaa aatgctttcg
     3661 ccgcgcatat cgtaggcagc gtcgatgccg gcattgcctt cgaggatgga actcaaatcg
     3721 ttcgtaccgt aatttttgtt tttctggata ttgagcgtat cgatggtttg cggcgtttct
     3781 ttgatgagct gtccgttgcg ggtaacggcg gcttcgtcgt agttgatgta gcctttgagt
     3841 acgctggtgt cggactgtcc gaccacggaa acggtgggca gagtggcggt gtaatgttca
     3901 ccattgtcct gcgtatcggc ggcagcaaca gggaaggaag caataatcag cgtgggtaat
     3961 aaagctaaat gaaatgatat tttcattttt atactcaatt taacaaaaca accgaattat
     4021 attgcctcac ggaggaaatg agaataattt cttttaacta tattgaacat gatatttgta
     4081 aacaaaggtc tcagaatgcg gaaaactcgc cgcctgatac tgaaaaatgc cgtctgaaca
     4141 gggttcagac ggcatttttt tgaccgcgaa attatgcgcc gaacactttc aaacgttctg
     4201 caacgggttc aaaggtcttt tcacctgccg ccccttcaat accgccgata accatttgtg
     4261 cgcgcaacaa ccagttttcg gggatattcc acgctttggc aatcgccgca tcgggcaagg
     4321 gattgtaatg ttgcaggttt gcacctacgc cgaccgcggc aagtgtcgtc caaacggcat
     4381 actgcaccat agcgttcgcc tggtccgccc aaacgggaaa gttggcggca taagcaggga
     4441 actgctcctg caaacctttg acaacatttt gatcttcata aaacaaaata gttgccgcac
     4501 ccgccttaaa caggttcaat ttttgcgcgg tcggttcaaa actgtcggca ggcacgacgg
     4561 cacgcagcgc gtcttcgaca aattgccaca ccttatcatg ctcttcgcca aacagcacga
     4621 ccacgcgggc agattgggaa ttgaacgaag aaggtgtgtg caaaacggcg tgttcgacga
     4681 tttggacaac ttcatctttg ccgacgggca ggtttttatt taacgaataa acggaacggc
     4741 ggctttcggc agcctgttgc agagattgac gggtcatttc ttttcctcaa aaacattcaa
     4801 caaaatacac tttaggcagt ccggttcagg ctttacgctt gaaaaacagc ctgtccagcg
     4861 accatgctcc gccgcccgct gccgcgatat agaggaatac gaagcagaac agcactgcgg
     4921 actcgccgcc gttggcaatc gggaacaaag catttccgga agcgtgcgcc ataaaatagg
     4981 caaccgccat ctgaccggac aaaacaaacg cggcagggcg cgcaaacagg cccaacacca
     5041 gcaaaatgcc gccgacaatt tctaaaatac cggcaagcag caacagcccg ccgggcgaac
     5101 cgctgcccat ttcaatgggg aaggcgaaga ttttcgacgt accgtgcaac aaaaacaggt
     5161 aggcggttac gatacgcaaa acagaaagca aaaccggttg gatacggttg cagcaatcgg
     5221 acataaaagc tcctttgata tttgtcttca tttcaggcgg tagagtatag atgtttcccc
     5281 ttttcaaata tatactgctt ttattgatac aacttttcca cacaagaaaa tatggacacc
     5341 ctgttcagcc tcaaggtttt ccgccaagtc gtccaaagcg gcggcttcac ccgcgccgcc
     5401 ggcgcgctcg gcatctccac cgcaatggca agcaaacacg tcagccactt ggaaaacacc
     5461 gtccaagcca cactcctgca ccgcaacagc cgcaacctca gcctgaccga agccggggaa
     5521 gaatactacc ggcaatgcag ttacgcgctc gacacgctcg acgatgccgc gcaaaaagcc
     5581 gccgggggga cggaaaaacc gcaggggctg ctgcgcgtaa cgatgccact gtggtttgcc
     5641 ggcagccaga tatgcaactg gctggcggaa taccgcgaac gttatcccga agtgacatta
     5701 gaactgattt tggacaaccg ccacgtcgat ttgattgccg aaggcgtgga tttggcgttg
     5761 cgcgtttccc aaaccctgtc cccgtcactg attgcgcgcc cactggcgga aatcgaattt
     5821 gccctgcttg cctcgcccga cttcctgagg cgcaacggcg tgccggaaac gccggaagaa
     5881 gtggcggggc tgcccgccgt cctgccgacc tacaccaacc agcagaaact cgacctcacc
     5941 cgcaaatcgg acggcaaaaa ataccggctt gaactgaccc ccgtcatccg taccgacaac
     6001 acgctgatga tgcgcgaaat gattaaggcg ggcgcgtgca tcggttatca gccgctttgg
     6061 gcggcggaac acgatttgcg ctgcggcacg ctggtgaggc tgctgcccgg atacgccgtc
     6121 ccgaccgacc ggctgaatgc cgtttatgca gacagggcat tcttaagcgc gaaagtccgc
     6181 agcttcatcg attttctgaa cgaaaaaatc gccagcagga aaggctgccg aaatgccgtc
     6241 tgaaaccgcc cgcccccttt atgcggacac gtgcgccgca cacacgctcg tctggttccg
     6301 ccaaaacctc cgcatccgcg acaacgccgc cttatgcacc gccgttgccg aaggtttgcc
     6361 cgttatcggt atttggattg acgatgccga aacagacaac cctcgccgcg ccgcgttcta
     6421 ccgccaatcc gccgccgaac tcgcccaagg gcttgcaggg cgcggcatcc cgctctacac
     6481 ggcggcatcg cctgccgagc tcgtccggct cgccgtccgc ctcaatatcc gcgccgtcat
     6541 cgccgacgaa tcccatactt ttgccgacaa actcgccgac aacgcccttt ggcatgaatt
     6601 ggacaaacac ggcatcgcgt taaccttcgt caatgaccgc gccgttttcg gcaaaaccga
     6661 cctgataccc gacggcggca cggcacacac cgatttcgac cgctaccgcg aagtatggct
     6721 cgaccgcttt tccaagcagc cccccgccgg tccggaccta ttcgcggcat accgccaacc
     6781 cttccccgaa aacctttccg ccccgccgcc tgccgccctt tcagacggca tcttcctgcc
     6841 gcaaaacagc ggcgaaacgg cggcttggcg gcagtggcgg cggtttctcg aacaggcgga
     6901 ttcctactcc gttttaaagg atttcccctc gcgcaaacac acttcgctga tgggcgcgta
     6961 tttgagtgcc ggctgcatct cgccgcgcct gctcgcgcgg gaaagcctcg aacgccgtct
     7021 gaacgcgtgg gcggacaaca tcatccgccg cgattttttc cttcaacttg ccttgcagca
     7081 cacggatgac gacccttcag acggcaatcc tgaacacacc ctgcgcctga cgctttggca
     7141 gcagggccgg accggcattc cgattatcga tgccgcgatg cgctgtttgc acaaaaccgg
     7201 cagcctccac cccgccctga gacgcttgag cgcggatttt ttctgccacg ttttaaacct
     7261 cccccgccgc gaaggcgaga tatggtttgc ccggcagctg accgatttcg atgcggcaat
     7321 caaccaaggc aactggtggc ttgccgcctc acggcacacc tgccccgaca ttgccgccgc
     7381 ctcataccgt accgacccgg acggtacttt tatcaaacgg cacattcccg aactcgccca
     7441 cctgtccgcc gacaccgtcc acacgccttg gcggtttgcc tgttcggtcg atacccacgg
     7501 ctatcccgcc catcctgtcg ccggtattcc ctaagcggta cggcaaccta aaacaaatgc
     7561 cgtctgaact tccgtttcag acggcattgc gtccgaatga ctcaatatct tccgtttttt
     7621 tcactttcaa tcatcaggta tgcccaaatc accgccaccc aagccgacac cgttcccatc
     7681 gccggcaaaa aatgctgcca atccgtcatc ggcggcacag ggcaacggcc gcattcgatt
     7741 tccgcgccgc ccaacaaccg gtacagcaac atatccgcaa tccaaagcaa caacgggtat
     7801 ttcaaacgct gcaaccaaaa aaccaaccac gtccgcacga ttcacctcac cccccattcc
     7861 gacaacgata ttgtaccgct tccgtgcaat gttaaaataa gcccacttct accgccgtac
     7921 aaaacgccat gctcaccgat ttagaaaaaa acgccatccg cgaccattac caaaacatcg
     7981 gcaaaaacct gcccggtttc cgtccgcgcg cttcgcagcg ggaaatgatt gcggcggttg
     8041 ccaacgcttt ttcgcggacg ttggcgcgcg aagaaggcgg cgagccgccc aagcgcgagg
     8101 gcgagagcat cgccgtaatc gaagggccga ccggcgtggg caaatcgctt gcctacctgc
     8161 tggcgggcgg catcatggcg cagacgcgcg gcaagcggct gattgtgagc agcgcaacgg
     8221 ttgccttgca ggagcagttg gtagaccgcg acctgccgtt tttggtcgaa aaaagcggtt
     8281 tggaactgag cttcgcgctt gccaaagggc gcggccgcta tctctgcccc tacaaactct
     8341 atcaactgac gcaaagcaat gcccagcaaa acctgctcgg ctttgaagcc cccgccgtct
     8401 tgtgggacag caaacccaag cccgaagaat tgaagctgct gcgcgacatt gccgacgaat
     8461 tttctgcccg acggttcaat ggcgaccgcg acgcctggcc ggaaaaaatc gacgacgcga
     8521 tttggctcaa agtgaccaac gaccgccacg gctgcctgaa aaccgcctgt cccaaccgtc
     8581 cggaatgtcc gttttacctg gcacgcgatg tcttggaaac cgtcgatgtc gtcgttgcca
     8641 accacgatct tctgcttgcc gacatcagta tgggcggcgg cgtgattctg cctgcgcccg
     8701 aaaacagttt ctattgcata gacgaagcgc accacctgcc caaaaaagcc ctcagccgtt
     8761 ttgccgccga acattcatgg aatattgccg tttggacgct ggaaaaactg ccgcagctga
     8821 ccggcaaaat tgccgcgctt accgataaag ccgaacttgc caacctggcc gacgaagccg
     8881 ccgcatcctt gctcgacagc ctgcacgaat ggcagttcca tttggcggaa gagccgtctt
     8941 taagtatggg gccgtctgaa aacgacagac gaaccaacag cgaaccgact tggctgtggg
     9001 aagacggcaa aatccccgaa ggcctcgaaa ccaccgtttc caatacggcc attgctgcgc
     9061 gcagcctgct caaacacgtt gtcgggctga acgatgcgct ttccgccgcg cgccgcgaaa
     9121 aagaacagga cggcgcgctc ctcgaccgcc tgaccggcga gttcggcatc ttcatcgtcc
     9181 gtatcgaaca aatcagcgcg gtttgggatt tgctctccac cgtccccctc gaaggcgaag
     9241 agccgctggc aaaatggata acccgccgtg ccgacgacaa aaacgactac attttcaacg
     9301 ccagccccat cagcagcgca tcccaccttg ccaacagcct gtggcggcgt gcggcaggcg
     9361 cggtattgac ttccgccacc ctgcaatcct tgggcaactt caacctgatg ctgcgccaaa
     9421 ccgggctgca atggctgccc gaaaccacca ccctcgccct caaaagcccc tttgactttg
     9481 aaaaacaggg cgaactctac atccccccca tatacgccag ccccaaagac cccgaagccc
     9541 acaccgccgc cgtcatcgaa tggctgccca agcttatttc gcccaccgaa gccatcggca
     9601 cgctcgtctt gttttcctcg cgcaaacaaa tgcaggatgt cgccctgcgc ctgcccggag
     9661 actacctgcc gctcttgctc gtacaaggcg aattacccaa agccgtcctc ctgcaaaaac
     9721 accaccgggc catagaagaa ggcaaagcca gcatcatctt cggactcgac agtttcgccg
     9781 aaggactcga cctgcccggc accgcctgcg tgcaagtcat catcgccaag ctgccgtttg
     9841 cgatgcctga caaccccatc gaaaaaaccc aaaaccgctg gatagaacag cgcggcggca
     9901 atccttttat tgaaatcacc gtccccgaag ccggcatcaa actcatccag gccgtcggcc
     9961 gcctcatccg caccgaacaa gactacggcc gcgtaaccat cctcgacaac cgcatcaaaa
    10021 cacagcggta cggccaacaa ttattggccg gcctgccgcc gtttaaaagg atagggtaaa
    10081 cataccgccc ttcccgcaac agaaaggaaa aattcatgaa ctttacccga ctgctcaacc
    10141 aagtcttaag cacggttcaa aaaaaaggcg gcacattctc cgacagcccg ctcaattcat
    10201 tcggcggagg cgcgctggtt gccggtatcg cctccatgct gctgaacggt aaaaaccgca
    10261 aaaccattac caaaatcggt tcgaccgccg ctttgggcta cctcgcctac cggggctatc
    10321 agatgtggca gcaaaacaaa gggcgggcaa ctgtaacaca aagcgatttc caacctgccg
    10381 gagaaactga agaaacatac agccgcaccg tattgcgcac catgatagcc gccgccgctt
    10441 cagacggcat gatagacgaa gccaaacgcc ggactatcga acaggaaagc ggcacagacc
    10501 ccgaaactgc cgcatggctc gccgccgaat accgcctgcc cgcaagcatc gaagacatcg
    10561 ccgccgccgt cggcaacgat gaggcgttgg cggcggaggc ctatctggcg gcaaggttgg
    10621 tctgtgccga tttgtcgcgg aaagaaaccg tcttcctcgc ccgcctgtcg caggctttga
    10681 aactggatga caatctggtg gagagtttgg aaaggcaatt ggggttttga tgacacagcc
    10741 ggggagggaa aatcatcaaa aaacggttat tccgaccccg gcctcaaaac ggcaaaatat
    10801 agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc
    10861 taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt
    10921 cgccttgtcc tgatttttgt taatccacta taaaagccgc cgccccgaag ggcaaacagc
    10981 ggcataggct gccaaaggca atatccgaca agaaggcagg ctactgcaat gcgttgccgt
    11041 gtgaatgctt gagcaagctt tcatgtttgt gaaattcccg ccccatataa taagaaccga
    11101 aataggtcag gtggtcttgg tcgccgtaaa gatagcggcc gtatatttcg accgtgtttt
    11161 tgggcaggta tttttgtgcg tccacccaat gcacattggg aatatcttta atcaaatcaa
    11221 agaccgcctg attgctcttg ccgatgtcgc ccatagcctg aatggggcgg agatattggt
    11281 ttgcggcaaa tcttttcaat ttttcctccc tcaggggcga acggctgatt gatgtgttgt
    11341 ttgcaaaaac atagacgggt ttgacggcgg ctatcctttt gacggtttcc ctgaatcggg
    11401 ctgggaaccc gggtattagg aaggattgcg cttcaaatct cggcacgggc tggccgccca
    11461 tcctcaaatc atagaattgg gcaatgaaaa cggcttcggc tttttcaact tcatcccggt
    11521 attttcgaca taacgggttg tctgccagct tctcatctac ccaaaccaaa cactccgaat
    11581 cgagggacag gattttggct ttccaccctt cccggctgcc gacataatcc agaaaacccc
    11641 gcaggtgtcc ggcgtgcgag tcgccgaggg tcaggacggt ttccggaaaa tgattttctg
    11701 cagcaagggg cgcgccgggc aacgggcgga ggtgttcctg tttcaatatc ccccttgcgt
    11761 acaggttgta accgacaagt atcagggacg gggcgagata gaggcagaaa aatgcctttt
    11821 tgaaggtcat cttccgtttt ctaagcggct gttcaatcaa ataataactc aacagggaaa
    11881 atccggccgt caacgcggca accgccgata cggcaggcag tccgagctgt ttgtcgcctg
    11941 taatgtaatg ggcgaaggca ataaaaatcc aatggtacag gtatagggaa taagagattt
    12001 tgccgacaaa tacgatgggg cttgccgaca ggatgcgggt cggaagtgtc ccgtattgca
    12061 tactccggat aagcagtgcc gtcagcaggc agggaaggag cagggtcatt cccgggataa
    12121 acggattgtg tttgtcaatc acgaacaggc aggcaagcaa tgcgccgaag cagagtgatg
    12181 aaagcaactg ccgttttcca tttgctgttt gccgtctgcc gttttgcgtt tgcccgtaaa
    12241 ccgccagcag cgaacctgcc aacagctcgg gaaacctcag tgtcgaaagg taataagtat
    12301 tgggttggtt gagaatatcg gtataaaacc cgcttggcaa aaacgatgag gcagtcaaaa
    12361 tcagaaatag gatgatgctg atgttacgca gcacccgtag cgattttgtt tttttgcagc
    12421 aaaatatcag caaaagagga tacaggaggt aatactgttc ctctactgcc aaagaccaga
    12481 tatgcagtac ggggttctcg tcggcactca aatcgaaata cccctgctga aaccccagat
    12541 aaatattgga caagaaaacc gcagaaagct ccacggtttt ccgcatttgg ttgaaatctt
    12601 cgtaaaggaa gatttgagag gcaatcaccg aagccagcga cacggccgca ataaaagcag
    12661 gataaatccg cttaatcctg cgggtataaa aatcccggaa agaaaaagaa ccgttctgta
    12721 tttcagaaag aatgatgccg gtaatgagga atcctgagat gacaaagaaa atgtccaccc
    12781 ccaggaatcc tccgggcagc cagcggtcat tcaggtggaa aataatgacg gatagcacgg
    12841 cgacagcccg taatccgtca atttcaggcc tgtatcggac agtttgcatc aatatcgccc
    12901 ccgtatgtcc cgttatctta aaaatgccgt ctgaacgcgc gttcagacgg catcggtttc
    12961 agtaaacgct caaatccgtt tcgccaacgc ttcagccttg cccacataca gtgcaggagt
    13021 cagctcaagc aattttgctt tggcttctac cggaatttcc aacgattcga taaagccttt
    13081 cagcacttcg ggcgtgatgc cgcctttgcc gcgcgtcagg tctttcagtt tttcgtaagg
    13141 attggcgaca ccgtaacggc gcattacggt ttgaatcggc tcggcgagca gctcccaagt
    13201 ggcatccaaa tcggcggcaa gcgcggcggg gttgggttcg agcttgttca gaccgcgcag
    13261 gtgggcggcg aaacccaata cggcatagcc cacgcctacg cccatattgc gcaatacggt
    13321 gctgtcggtc aggtcgcgct gccagcggga aatcggcagt ttttcggaca aaaagcccaa
    13381 tacggcgttt gccataccga ggttgccctc ggagttttca aagtcgatgg ggttgacttt
    13441 gtgcggcatg gtggaagaac cgacttcgcc tgctttgact ttttgtttga agtaacccaa
    13501 tgaaatataa ccccaaacgt cgcggttaaa gtcgatgaga atcgtgttga tgcggctgag
    13561 ggtttggaag aattccgcca tatagtcgtg cggttcgatt tggatggtgt aggggttgaa
    13621 ggtcagaccg aggctgattt cgacgaagtt gcggcagtgg gtttcccaat ctacatcagg
    13681 ataggcgacc atatgggcgt tgtagttgcc gaccgcgccg ttgattttgc cgaggaactc
    13741 ttgcgcttgc aggtttttaa actggcgttg caggcggtac acgacattgg cggtttcttt
    13801 gcccaaagtg gtcggcgtgg cgggctggcc gtgggtgcgg ctcatcatcg ggacggcggc
    13861 aaggtcgtgc gccatagcgg tgagtttgcc gatgatttct gccagtttgg gcagcaaaac
    13921 agcctcacgc gcttcttgca gcattaaagc gtgggacagg ttgttgatgt cttcgctggt
    13981 gcaggcgaag tggatgaact cactcacggc ggcgacttcg ggcacttcgg caaaacgttt
    14041 tttcagccaa tattcgatgg ctttgacatc gtgattggtg gtggcttcga tggctttgac
    14101 ggcggccgcg tcttccaatg aaaagttttc aatcaccgtg tcgatttcgg caagcgtttc
    14161 ggcactgaag ggcggcactt cggcaatctt cggctcggcg gcgagggctt tgagccagtt
    14221 taattcgact ttgacgcgcg ctttcatcag gccgtattcg gaaaaaatcg ggcgcaatgc
    14281 ttcaacggat tgggcataac ggccatctaa aggggaaagc gaggcgatgg ggttgatcat
    14341 ttcggcatcc tgttcgggaa agacatcaaa aattgttaaa ttgtttgcaa ttatacatca
    14401 ctctcaggac gctatgccgt ctgaagcccg tgttccgaca gatacgcaac gatttcgtct
    14461 tcatcgtccg atatggcaaa caaagagacc gccccttcgg aaatcagacc gcgcgccaaa
    14521 agctgcgcgt ttatccactc cgccaagccc gaccaaaacg cctttccgac caaaacaatc
    14581 ggacgcggcg gcactttgcc cgtctgcacc aaggtcagga tttcaaacaa ttcgtccagc
    14641 gtcccgaagc cgcccggcat cacgacatat gcttgggaat agcggaaaaa caccgccttg
    14701 cgttcggcaa aacgggaaaa ccgcaaggcg atgtcctgat acggattcgg tttctgctcg
    14761 tgcggcaaaa cgatgttcag ccccaccgaa accgacttcc ctgcaaacgc gcccttgttt
    14821 gccgcctcca taatccccgg cccgccgccc gaaatgacgg caatgcccga atccgacagc
    14881 cgccgcgcca gacggcaggc gaacgcataa tccgcatgat tctgcggcgt gcgcgcgctg
    14941 ccgaagatac tgactgccgg aaacacgccc gccaatgctt cgtctgcctg cctgcgttcg
    15001 gcatcataac gtgcctgctc cggcacacgg tttgtattct ccattccatc ctccgttcaa
    15061 aaacagcgat tgtacaccgt caaaaa
//
LOCUS       DAXIEB010000045        14851 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000045 DAXIEB010000000
VERSION     DAXIEB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.045"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            207..1049
                     /locus_tag="ACFOA3_001101"
     CDS             207..1049
                     /locus_tag="ACFOA3_001101"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6898118.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFQPVGTTDSERAFCHILNRLRTRFVARPDDDTLFGAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
     gene            1079..1543
                     /locus_tag="ACFOA3_001102"
     CDS             1079..1543
                     /locus_tag="ACFOA3_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6898119.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETARLTELRLYASGLRD
                     ISAAELVEIVLEELFGQSTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1575..2684
                     /gene="ribD"
                     /locus_tag="ACFOA3_001103"
     CDS             1575..2684
                     /gene="ribD"
                     /locus_tag="ACFOA3_001103"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6898120.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAQDSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLESAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLHPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            2879..4300
                     /locus_tag="ACFOA3_001104"
     CDS             2879..4300
                     /locus_tag="ACFOA3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6898121.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYTAVRFIVVSCMLPP
                     LFCTLAEISGIGLNVVRKTRPIALATLGALAANLLLLGLSVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4297..5369
                     /locus_tag="ACFOA3_001105"
                     /pseudo
     CDS             4297..5369
                     /locus_tag="ACFOA3_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            5389..6555
                     /locus_tag="ACFOA3_001106"
     CDS             5389..6555
                     /locus_tag="ACFOA3_001106"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6898122.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYGKNVSLERVASHYRFVKNFEQWLKNCRPGE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPPRFRSKTVRL
                     FYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYLP
                     YSEMMSIAKACDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQNNAEVLDLMNLLPH
                     ENYRSGDVDGFVQAAKDILKRKNDPVQSEEIVRRFKRDISYQKIVNLIERLANE"
     gene            6548..7075
                     /locus_tag="ACFOA3_001107"
                     /pseudo
     CDS             6548..7075
                     /locus_tag="ACFOA3_001107"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            7124..8125
                     /locus_tag="ACFOA3_001108"
     CDS             7124..8125
                     /locus_tag="ACFOA3_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6898123.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITVQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            8112..8799
                     /locus_tag="ACFOA3_001109"
                     /pseudo
     CDS             8112..8799
                     /locus_tag="ACFOA3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            8847..9041
                     /locus_tag="ACFOA3_001110"
     CDS             8847..9041
                     /locus_tag="ACFOA3_001110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898124.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHNRR
                     GQSGKAAAAQKPRDLDSISD"
     gene            9165..10340
                     /locus_tag="ACFOA3_001111"
     CDS             9165..10340
                     /locus_tag="ACFOA3_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6898125.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAVIVVHLAGMPAEMDGIMTLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLAEATR"
     gene            10388..12298
                     /gene="pglD"
                     /locus_tag="ACFOA3_001112"
     CDS             10388..12298
                     /gene="pglD"
                     /locus_tag="ACFOA3_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6898126.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAIYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            12354..13646
                     /locus_tag="ACFOA3_001113"
     CDS             12354..13646
                     /locus_tag="ACFOA3_001113"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6898127.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13698..14534)
                     /locus_tag="ACFOA3_001114"
     CDS             complement(13698..14534)
                     /locus_tag="ACFOA3_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6898128.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSSGILGACPELPENCRPVWAHFS
                     RH"
BASE COUNT         3417 a         4594 c         3720 g         3120 t
ORIGIN      
        1 ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa gtcggattcc cgcctgcgcg
       61 ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt ttctgttttc gagggaataa
      121 tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc
      181 gaccatctga atcatttgga caaactatgt gtcaactgct gggcatgaac tgcaatacgc
      241 cgaccgatat tatgttttcc tttgaaggct tccgccggcg cggcggcatt accgaccatc
      301 atgccgacgg tttcggcatc ggctttttcg aaggcaaagg cgtgcgcctg ttccatgacg
      361 acaaacccag tgccaattcg cccgtcgccg acctcgtgcg cgcctaccag attaaatcgg
      421 aaaacgtcgt cgcccatatc cgcaaagcat cgcaaggaca aacctcgctg gcaaacaccc
      481 atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac gggcatttgg
      541 tcgacttctt tcccgaacag ggcgaatttt tccaacccgt cggcacaacc gattccgaac
      601 gcgcgttctg ccacatcctc aaccgcctgc gcacacgctt tgtcgcccgt cccgacgacg
      661 acacgctgtt tggcgcgatt gcggggctga cgcacgaaat ccgcaaattc gggctgttta
      721 actttatgct ttcagacggc attgccctgt ttgcccacgc cagcacactg ctgcactaca
      781 tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga cgacgacgta atggtcgatt
      841 ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgacgc
      901 gcgatgaaac gtgggcgcaa cttgccgttg acgaactggt gatgttccgc gaaggcaaaa
      961 ttattttgaa cgaccgaccc cggcaccccg tttatatgag tgtcgaagaa ggtttggaaa
     1021 ccgcgcgcgc cgccggcgtc gccgtctgaa cttcagacga cataggagga cgaacccgat
     1081 gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc gattcgcgcc tgatggacga
     1141 acgcaacgcc gtccgccgcc gccgatactg ccccaactgc ggcaaacgct tcggcacact
     1201 cgaaaccgcc gaactcaaaa tgcccgccgt catcggcacg gacaaaaaac gttcgccctt
     1261 caatgcacaa cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc
     1321 cgaacagatc gacgaaaccg cccgcctcac ggaactcagg ctgtatgctt cgggtttgcg
     1381 cgacatatcc gctgccgaac ttgtcgaaat cgtactggaa gagctgttcg ggcaaagcac
     1441 ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc gacaatccgg cagactttgc
     1501 ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact
     1561 gatacggtat ccccatgttt tcggacacag atatatccat gatggaaaac gccctccgac
     1621 ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac
     1681 aagacagcca aattgtcggg cagggcttcc acgtcaaagc gggcgaaccg cacgccgaag
     1741 tccacgccct gcatcaggcg ggggaaatgg caaaaggcgc gaccgccttt gttaccctcg
     1801 aaccgtgcag ccattacggg cgcacaccgc cctgcgccga agctctgctc cgttccggcg
     1861 taacacgcgt cgttgccgcc atgcgcgacc ccaacccgct ggttgccggc aaagggcttg
     1921 ccctgctcga atcggcaggc atcaaaacag aatgcggact gcttgaaaac aaagcacgcg
     1981 aactcaaccg gggattttta tcgcgcatcg aacgccgccg tcccttcgtc cgcctcaaat
     2041 gcgccgtttc gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg
     2101 gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg
     2161 gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc
     2221 gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct gcccttggac agccaccttc
     2281 tgaccgacgg acaatctcca acctacatcg ccaccttgga acgcgatgaa gacaaactcc
     2341 acccctatcg caaacacgcc cacgtccgca tcctgatgcc gtctgaaacg gcaggcggca
     2401 aaatcgattt gaacagcctg atgcgcctgc ttgccgacga aggcataggg gaactcatag
     2461 tcgaagcagg ctccgaactc acatctgcat ttttggcaga agatttggca gacgaaatcg
     2521 tgctgtaccg ttcgcccaaa atcctcggca gcggcaaaga cctgctttcc ctgcccgaaa
     2581 accgcgccgc cctttccgca ccgcccttgt ggacacccgt ttccaccgaa atcctcggac
     2641 acgacatcaa aaccgtgttt cggaaaaacg ataacgcctt ttaaagggtt tgcgccgttt
     2701 cactatataa taacgccgat aaaaaacggc accattcaga ccgccgccca cccgaaaaag
     2761 cgcaacccgg actgccgcac cccgccggca gccgcgacgg tctgaaagcc gtcaaattcc
     2821 gatcaagaaa ggcttcagac ggcataggca gcatcccgcc tccgtccgga catcaaaaat
     2881 ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt
     2941 catcatcctg ccgctgctgt cgtggtattt ccccgccgac gacatcggac gcatcgtgct
     3001 gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc ctcgggctgg atcaggcata
     3061 cgtccgcgaa tactatgccg ccgccgacaa agacaccttg ttcaaaaccc tgttcctgcc
     3121 gccgctgctg tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc
     3181 tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact
     3241 gagcttcctg cccatccgct ttctcttact ggttttgcgt atggaagggc gcgcccttgc
     3301 cttttcgtcc gcgcaactcg tgcccaagct cgccatcctg ctgctgctgc cgctgacggt
     3361 cgggctgctg cactttccgg cgaacaccgc cgtcctgacc gccgtttacg cgctggcaaa
     3421 ccttgccgcc gccgcctttt tgctgtttca aaaccgatgc cgtctgaagg ccgtccggcg
     3481 cgcaccgttt tcatccgccg tcctgcatcg cggcctgcgc tacggcatac cgatcgcact
     3541 aagcagcatc gcctattggg ggctggcatc cgccgaccgt ttgttcctga aaaaatatgc
     3601 cggcctagaa cagctcggcg tttattcgat gggtatttcg ttcggcgggg cggcattatt
     3661 gttccaaagc atcttttcaa cggtctggac accgtatatt ttccgcgcaa tcgaagaaaa
     3721 cgccccgccc gcccgcctct cggcaacggc agaatccgcc tccgccctgc ttgccgccgc
     3781 cgtctgcctg accggcattt tctcgcccct cgcctccctc ctgctgccgg aaaactacac
     3841 cgccgtcagg tttatcgtcg tatcgtgtat gctgccgccg ctgttttgca cgctggcaga
     3901 aatcagcggc atcggtttga acgtcgtccg caaaacacgc ccgatcgcgc tcgccacctt
     3961 gggcgcgctg gcggcaaacc tgctgctgct ggggctttcc gtaccatccg gcggcgcgcg
     4021 cggcgcggcg gttgcctgtg ccgcctcatt ctggctgttt tttgccttca agaccgaaag
     4081 ctcctgccgc ctgtggcagc cgctcaaacg cctgccgctt tatctgcaca cattgttctg
     4141 cctggcctcc tcggcggcct acacctgctt cggcacgccg gcaaactacc ccctgtttgc
     4201 cggcgtatgg gcggcatatc tggcaggctg catcctgcgc caccggaaag atttgcacaa
     4261 actgtttcat tatttgaaaa aacaagggtt tccattatga aaatcatcct gaccacttct
     4321 atggcaggct tgggcggcac ggaaaccgcc accgtccgcc tcgggcggct actcaaacgg
     4381 cgcgggcacg acgtcatcct tgcttcttcg gacgggcctt ttgtcggaga agcgcaagca
     4441 tcgggcatcc gttggcagcc ggttgatttt taccggggcg ggcttgccgg ctacctcaaa
     4501 agcaccttta cctacgcccg gatgctgcgc cgcgaacaac ccgacatcat cgactgtcaa
     4561 atggcgcgcg tcgttcccgc ctgcgccctt gccgccaaaa tcgtttcgcc gaaaaccaaa
     4621 atcatctacc actcgcacgg tttggacgcg gcaacctacc ccaaaatcgc caaactcttc
     4681 gacaagttgg gcgtgtacat catcggcaac tgcaaacacg agcgcgaaaa actcatccgc
     4741 cacggcttcc ccgccggccg gattgcctac gcctacaacg cactcccccc cccccggaat
     4801 ttcatttccg gaaaaccgaa aaagaatgcg ccgtactcgg cacgctttcc cgtttggaca
     4861 ccgtccgcgc cgtgcatctg atgttggata ttttgaagaa aatggttgac cgaggtatgc
     4921 ccgtccgcct caacatagcg ggcataggag aagaaatgga caatttgaaa acgcaggcga
     4981 agcgtctggg cattgaagac aaaattacct tcctcggcgg catacgcgac ttgaccggct
     5041 acttcaaaga aatcgatatt ttggtgaaca cgccgcattg cgtgggcgac cacggtgcag
     5101 gtgtcggcaa caatattttg gaagccggcc tttacgacac gcctgtcgtg acctacgaca
     5161 tggcgggtat ttccgaaatg gtcgtcaacg gagaaacggg ttactgcatc cccttcggcg
     5221 acgaagaagc ctttatcgcc gccgtagaca cgctcgtcag acagccgctg ttgcgcgaag
     5281 caatgggcaa aaggttgcac gcacacgtcg ccgcactctg ttccgacgac gaaatctacc
     5341 gtaccaccat ggcggcgtat accatgtaat gccgtctgaa agccgccgat gaacatcacc
     5401 atagtcgccc cttattgctc gctgccgtcc gaaccgtatt tcaaccgctt ttggtatctg
     5461 gcagaacgat tgtcgcaatc gcacgacgtg ttgctgatta ccagccgttt ccgccactac
     5521 gacaaatcct tcagacggcc cgaagatgcc gaggccgcct cgcaaggccg tctgaaagtc
     5581 atgctgttgg aagaaagcgg ctacggcaaa aacgtgtctt tagaacgtgt tgccagccat
     5641 taccgcttcg tcaaaaattt tgagcaatgg ctgaaaaact gccggccggg cgaacaagac
     5701 atcgtctatt ccgcctatcc gctgatggca accaacctgc tgttgggcaa acacaaagcg
     5761 cgtttgggct acaaactgat tgtcgatgtg caggacgtat ggccggagtc tttctcctcg
     5821 gtcgtaccgt ttttgaaaaa agtaccgcac aagctgctgc cctttgcttc acgcgccaac
     5881 cgtgcctacc gctgcgccga cgcgctggtt gccgtatcgc agacttatct cgaccgcgcc
     5941 aaagaagcca atccgaacgt acccggcgaa gccgtctata tcggtacgga ttttgccgcc
     6001 atcgcccccc ccccgcgatt ccgctccaaa accgtccgcc ttttctactt gggcacgctc
     6061 agttacagct atgacgtgga aaccgtgtgc aaaggcgttc ggaaactttt agacgacggc
     6121 gaaaacgtcg aattgcacat tatgggcgga ggacccgatt tggagaaact caaacaatat
     6181 gaaaatcgcg ccatcaagtt ttacggttat ttaccctatt ctgaaatgat gtcgattgcc
     6241 aaagcgtgcg acattgccgt caacgccatc catagccacg ccatgcagtc ggttaccaac
     6301 aaactctccg actatatggc tttgcaaaaa ccgattctga acagtcagaa caatgccgaa
     6361 gtcctcgacc tcatgaatct gctgccgcac gaaaactacc gttccggcga tgtggacggt
     6421 ttcgttcaag ccgccaaaga tattttgaag cgcaaaaacg atcctgttca gtccgaagaa
     6481 atcgtccgcc gcttcaagcg cgacatttcc tatcaaaaaa tcgtcaacct gattgaaaga
     6541 ttggcaaatg agtaaagccg tcaaacgcct gttcgacatc atcgcatccg catcgggact
     6601 gattgtcctc tcacccgttt tcctgatttt ggcgtacctc atccgcaaga atctgggttc
     6661 gcccgtcttc ttcattcagg aacgccctgg aaaggacgga aaacctttta aaatggtcaa
     6721 attccgttcc atgcgcgacg cgcttgattc agacggcatt ccgctgcccg acggagaacg
     6781 cctgacaccg tttggcaaaa aactgcgtgc cgccagcttg gacgaactgc ccgaattatg
     6841 gaatgtctta aaaggcgaga tgagcctggt cggcccgcgc ccgcttttga cgggctacct
     6901 gccgctttat aacgacttcc aaaaccgccg ccacgaaatg aaaccgggca ttaccggctg
     6961 ggcgcaggtc aacgggcgca acgcgctttc gtgggacgaa aagttcgcct gcgatgtttg
     7021 gtatatcgac cattacagtt tttggctgga tatgaaaatc ctgtttttga caatcagaaa
     7081 agtcttgatt aaagaaggca tttcggcgga aggggaagcc actatgcctt atttcactgg
     7141 aaatcaaact aatgaaaaaa ataatatact gattctgtcg gcaggtcgcc gtgtcgaatt
     7201 ggtgcaggac ttccaaaccg aagccgcacg tttttcagac ggcatcagcg tttttgccac
     7261 tgacctcaac ccccgcatgt cttctgcctg ccatgtttcg gacggttcat ttgaagtgcc
     7321 gccgattagt gccgattcat atatcgacag cattttcaat ctggcggtac gcgaaaatat
     7381 cggtttaatc atcccaacca tagacaccga actgcaaaag ctggcagacg agcgcgaacg
     7441 ctttgaagcg gcaagtgtcc acatcgtcgt ttccgattcg gacttcatcg cacaatgccg
     7501 cgacaaacgg aaaaccgcca ccttgtttgc ccgctacggc atacgctcac ccgaaattta
     7561 cgaccgcaag aagttggttt tcccttgctt cgccaagcct tatgacggca gccgcgccat
     7621 cggtgcaaaa cggattgaca cacctgccga cctgacaccg gaagtcttgg cagacaccaa
     7681 attaatgttc tgtcagttta tcgacatcga aaataaattt tccgagttta ccgtcgatat
     7741 gtattacgac cgtcaatgcc gtctgaaatg cgccatcccg cgaaaacgct tggaagtgcg
     7801 ctccggcgaa atcagcaaag gcgttacctg caaaaacagc ctctaccaaa cgctgctgga
     7861 aaaaatgtcg gtattgcagg gcgcgcgcgg ctgtatcacg gtgcagttct tctacaacga
     7921 agaaaccggc gaattctacg gcgtggaaat caacccccgc ttcggcggtg gcttcccgct
     7981 gacgtatgcc gcaggcggca attatccgag ccggttgatg cgcgaatacc tcggcgacga
     8041 agaaatcccg ttttcagacg actgggaaaa caacctcatt atgttgcgct acgatgccaa
     8101 ggtcttggtt catgaaaacg attaatcctg aaaccaccgt catcgtcttc gacttggacg
     8161 ataccctgta ttcggaatac gaatacaagc tctccggtat ccgttcggtc atcgataccg
     8221 ttgccgcact gtatcccgat tggaattcag acaacctatg gcgcaacatc gaccccgacg
     8281 gcaaagattg gctggacaaa ttgtgccgcc actgtggttt taatgaatcg gaaaaacagg
     8341 ttctttctgt ggcaataccg gctgcaccgc ccgacgctga ctccgtatgc tccgccggat
     8401 ttcctgtccg aactgaccgc gcccttcgcc gcacgcgcgc tgattaccga cggcagaagc
     8461 ctgacccaac gcctgaagct ggaagccttg ggtctgtctt ccctgtttga cgacatcctc
     8521 atctctgaag cctgctcttc ggaaaagccc gacggcaaac gcttccgcca tcttcaagac
     8581 aaatatgccg acaaggcagg ctgcttcatc tatatcggcg acaacatttc caaagacttt
     8641 atcgcgctga acactttggg ctggataacc ataggattgc ttccctccgg acagaacatc
     8701 caccgccacc agcctgaaag ttttaacaca gaatatcatc ccgacttttg ggttaactcc
     8761 cttcaagatt tagttcctat aattgattca aatacttaac cattacataa tcaaacatgc
     8821 actcacaata ttaaaaggac tatttcatga caaaatatta ttcatggggc gtaaacggct
     8881 ttccgcccta cgttttcgac accatttgga aacgatggaa gttgaatggg cgcgggggcg
     8941 gttatcgtgt gcgacatccc ggacggcata accgtcgcgg gcaatccggc aaagccgctg
     9001 ccgcgcaaaa accccgagac ctcgacagca taagcgatta aaatacaccc ccgtacagac
     9061 cgattttgac aacacctgcg gcgcgcgccc gattcttcgg aacacgcccc cttcagacgg
     9121 catagggtcg gaaatgccgt ctgaaaaccg acggacaaac catcatgctg aacactttcc
     9181 tttccccgtg gccctgcttc acccaagaag aagccgatgc cgtttccaaa gtcctgctgt
     9241 ccaacaaagt caactactgg acgggcagcg aatgccgcga atttgaaaaa gaatttgctg
     9301 ctttcgccgg cacgcggtac gccgtcgccc ttgccaacgg cacgctggca ctcgatgtcg
     9361 cgctcaaagc aatgggcata ggcgcgggcg acgatgtgat tgttacctcg cgcaccttcc
     9421 ttgcctccgc atcctgcatc gttaacgcag gcgcaaaccc cgtgtttgcc gacgtggatt
     9481 tgaacagcca aaacatcagc gcggaaaccg tcaaagccgc gctgacgccg aataccaaag
     9541 ccgtcatcgt cgtccacctt gccggtatgc ctgccgaaat ggatggcatt atgactttgg
     9601 caaaagaaca tgatttgtgg gtgattgaag actgcgccca agcgcacggc gcaaaataca
     9661 aaggcaaatc cgtcggctct atcggacacg tcggcgcgtg gtcgttctgc caagacaaaa
     9721 tcatgaccac cggcggcgaa ggcggtatgg ttacgaccaa cgacaaaacc ctgtgggaaa
     9781 aaatgtggtc gtacaaagac cacggcaaaa gctacgatgc cgtgtaccac cgcgaacacg
     9841 cgcccggttt ccgctggctg cacgaaagtt tcggcacaaa ctggcgtatg atggaaatgc
     9901 aggcggcaat cgggcgcatc cagctcaaac gcctgcccga atggactgcc tgccgtcaag
     9961 cgcacgccgc caagctggcg gaaagcctag gcaaattcaa aagtatccgc ttggttgaag
    10021 tcgccgacta catcggacac gcgcaatata agttctacgc cttcgtcaaa cccgaacacc
    10081 tcaaagacgg ctggacgcgc gaccgcatcg tgaacgaact gaacgcacgc aaagtcccct
    10141 gctatcaagg cagctgctcc gaagtctatt tggaaaaatc tttcgacgac acgccttggc
    10201 ggccgaaaga gcgtttgaaa aatgctgtcg aactgggcga caccagcctg atgttcttgg
    10261 tgcacccgac gctgaccgac gacgaaatcg cgttctgcaa aaaacacatc gaagccgtat
    10321 tggcagaagc cacacgataa cccttcagac ggcatatgcc gcctgaaagc acataccgcc
    10381 ccacgatatg actctggaaa ccctgatcgc cctgccgcgc aacatcaaga aaatctgttt
    10441 cctcatacac gattttctga tgattttcat tgcattctgg ttcacccaaa gcctaaaggc
    10501 cgactactcg aacgaatggt tcgaccctgc caactggcag tctttcctat tgaccgcgcc
    10561 gttgaccatc ggcctgttcg tcagaatggg gctctaccgt gccgttacac gcttcatcag
    10621 cttccgcatc ctctccaccg cgctggcagg cagcctcgcc tccgccgtat tgttcttcct
    10681 caacacattg atttttgaag aaaggctgcg cctcgccctg ccgattgtct atttcttact
    10741 gctgtttgtt tccgtgaccg gctcgcgtat ggttttgcgc ggactgttgt ccgaacaccc
    10801 caaaaaacag atgatccccg tcatcattta cggcgcggga cggtcgggca gacaactgct
    10861 tgaggccgtc aaacagatac gcgaatattc cgcggtcgcc tttgtggacg acaaccccaa
    10921 gctttggcac accgtcatct acgaccttgc catttaccag cccgatgcca tcgccttcct
    10981 catcgaacgc tacggcgtgg aaaaaatcct gctcgccatc cccggcgcga cccaggaaca
    11041 acgccgccga atcatcagca aactggaagc ctatccctgc gaagtgttga ccattcccgg
    11101 aatgaaagac ctgatggacg ggaaaatcag catcggcacg ctcaaaaaaa tctccgtgtc
    11161 cgacctgctc gggcgtgatt ccgtcgcgcc cgacaaccgc ctcatgagtg ccgacatcga
    11221 aggcaaaacc gtcatggtaa ccggcgcggg cggctccatc ggttcggaac tctgccgcca
    11281 gattatccgc cgccgccccg aaaagctgct gctgtttgag ttatccgaat tcgccctgta
    11341 cgccgtcgaa aaagaattgc gcgaaacctg catccaaaaa cgcctcgaca ccgaaatcct
    11401 gccctttctc ggttcggtgc aaaaccgcac gctgctcgaa cacgtcatga ccgccttttc
    11461 cgttgcgacc gtctatcacg ccgctgccta caaacacgtc cccatggtcg aattcaacac
    11521 cgtcgaaggc atacgcaaca acattttcgg cacactcgag tgcgcgcttg ccgccacgac
    11581 atcgggcgta agaactttcg tcctcatctc caccgacaaa gccgtccgcc ccaccaacac
    11641 catgggtgcc agcaaacgca tggcggaact ctgccttcag gcactcgccg ccgaacccgg
    11701 acaaaaaacc cgcttcagca tggtacgttt cggcaatgtt ttaggttcgt ccggctccgt
    11761 tgtcccgctg tttgaaaaac agattgcaga aggcggcccg cttaccctga cccaccccga
    11821 catcacacgt tatttcatga ccatacccga agccgcccaa ctcgtcatac aggcaggcgc
    11881 gatgggtacg ggcggcgacg tattcgtcct cgatatgggc gaatccgtca aaatcatcga
    11941 ccttgcccgc caaatgatta ccttaagcgg cctcaaaccc aaaacacccg aacaacccga
    12001 cggcgacatc gaaatcctca ttaccggact gcgtcccggc gaaaaactct acgaagagct
    12061 gctcatcggc gacaacgtcc gcaaaaccgg ccatccgcgc attatgacgg ctgacgaggc
    12121 gatgctgccg tggcacgagc tctctgtcct gctcgaccgc atccgtgcgg cctgcgaccg
    12181 ttacgaccag caggcaatcc gcaccctgct catcaacgcc ccgaccggct ttaccccaag
    12241 cgacggtatc tgcgacctgc tttgggtacg agaaacacac agaaaaaatg ccgtctgaac
    12301 cttcagacgg cataacgtac aaaccaacct gcctgacaca cacggagttt gatatgcagt
    12361 tttcggcatt cggcgaaaaa ttcacgcaac acagcggcat cctgcaactg atggacgacc
    12421 tcggcgacgc gctcaaaagc gacaagcccg tcaatatgct cggcggaggc aacccggcac
    12481 gcattccgga aatcgatcgg gcgttcgccg acatattctc caaactggcg gcggaacacg
    12541 ccgtcgaaaa catcggcaac tactccaatc cccaaggcga tgccgcgctg attgacgcgc
    12601 tgaccgcctt cctcaaccgc gaatacggct ggaatttgac cgccgacaat atcgcgctga
    12661 ccaacggttc gcaaaacgcg tttttctatt tattcaacct cttcggcggc aaattcaagc
    12721 tttcagacgg cacatccgca gaaaaagcca ttttgttgcc gctcgcgccc gaatacatcg
    12781 gctatgccga cgtacatgtc gaagggcagc acttcgtttc tgtcaagccc aaaatcgaaa
    12841 acgtcgaaca cgaaggcgaa gcaggcttct tcaaataccg cgtggacttt gacgcactgg
    12901 aaaacctgcc cgaactcaaa gcgggcaaaa tcggcgcgat ttgctgttcg cgcccgacca
    12961 acccgaccgg caatgtgttg accgacggcg aaatggcacg tttggacgct ttggcgcgtg
    13021 aacacgggat tccgctgatt atcgacaacg cctacggaat gccgttcccc aacatcattt
    13081 acagcgacgt aacgctgaat tggcacgaaa acatcatcct ctgcttcagc ctttccaaag
    13141 tcggcctgcc gggcgttcgc accggcatca ttgtcgccgc gcccgaagtc gtcaaagccg
    13201 tcagcagcct gaacgcgatt gtgaaccttg cccccacgcg cttcggcgcg gccatcgccg
    13261 ccccgctact ggaaagcggc gagatgaaac ggcttgccga ccaagtcatc cggccgtttt
    13321 accgcaatca ggcgcaaacc gccgtctcgc tgctcaagcg cgagctgggc gcgtacccga
    13381 tgaaaatcca caaacccgaa ggcgcgattt tcctgtggct ctggtttgaa aacctacccg
    13441 tttcttcgca aaccctgtac gaaatgctca aagccgaagg tacgctgatt attccgggcg
    13501 agcatttctt cgtcggcatc gacacacagg attacccgca cgcgggcgag tgcatccgca
    13561 tgagcatcgc gcaggacgct caaacgctgg aaaaaggcat tgccgccatc ggtaaaaccg
    13621 tccgaaaact gtacgacaac gtttaaaccg caaaaaatgc cgtctgaaag cctttcagac
    13681 ggcattttta tccgcattca atgtcgggag aaatgcgccc acaccggcct gcagttttcc
    13741 ggcagttcgg gacacgcgcc gaggatgccg ctgctgaagt cgtttaaacg gtggaagtcg
    13801 ctgcccgcac tggcgagcat gccgaagcgt tctgccaaaa atgcgtagtt gaggcggtcg
    13861 tttttgcagc agttgccgct gtggacttcg atgcccgcgc cgccgaggtt tttaaattct
    13921 tcaaacagat tgcgcttggc ggtagcggac aaatcatagc gcatcggatg ggcgatgact
    13981 gccatgccgc ccgcgccgtt gacggcggag acgcagtctt ccagcgtcgc ccattcgtgt
    14041 cggacggcgc aggatttgcc gtcgcccaag tatttggtga acgcctgctg cttgtttttg
    14101 acatgtcccg cccggatgag gaactcggcg acgtgggtgc ggctgaccat ttctttgttt
    14161 gccgccagcg cgagcgcgcc gtcgtatgcg ccgccgatgc ctttcttttc gagcttggcg
    14221 gcgatggctt cgaggcgttt cagacggcct tgccgcactt gcgccaacag gttttgcagg
    14281 ttttcgtctt gttcgtcaaa gtctaagccg acaacgtgta tggtacgccc gcgccacgtt
    14341 acggagattt ccacgccgtt aatcaggcgc aaaccgagcc tgtcggcttc ggcacgcgct
    14401 tcggcgatgc cgccggtgtg gtcatggtcg gtcaacgcca gcagcgtgca gccgttttga
    14461 tgcgcgaggc gcacgacttc ggcgggggaa agcataccgt cggaaacggt ggaatggcag
    14521 tgcaggtcta tcatggggtg ttatatgtgt ggtgaatgaa ggtcgggggg caatattgtc
    14581 gggcagtgtg gcggcggcgg tcattttaaa aaacaggtgt atggcaaatg caaagaaaaa
    14641 gtccctatac cgtcattccc gcgcaggcgg gaatccagac tttgatttat tggaaatgtt
    14701 taaggttaac cgctatttca aacttccgga ttcccgcctg cgcgggaatg acgataggga
    14761 tgttttcaat tttaatctac tataaaagac tgtctgaaaa cgtggtttta tagtgaatta
    14821 aatttaaacc ggtacagcgt tggctcgcct t
//
LOCUS       DAXIEB010000046        14661 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000046 DAXIEB010000000
VERSION     DAXIEB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.046"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(95..658)
                     /gene="orn"
                     /locus_tag="ACFOA3_001115"
     CDS             complement(95..658)
                     /gene="orn"
                     /locus_tag="ACFOA3_001115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6898129.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVSGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(676..1563)
                     /gene="prmA"
                     /locus_tag="ACFOA3_001116"
     CDS             complement(676..1563)
                     /gene="prmA"
                     /locus_tag="ACFOA3_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6898130.1"
                     /translation="MSYQQITINVNDAVAERLADTLMEHGALSAAIEDAYAGTENEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAASIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQAQFDPIQISERLWITPSWHEAPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(1668..3029)
                     /gene="accC"
                     /locus_tag="ACFOA3_001117"
     CDS             complement(1668..3029)
                     /gene="accC"
                     /locus_tag="ACFOA3_001117"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6898131.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGLIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKVCVVG
                     KDRDQAMAKMRVALAELAITGIKTNTPLHRDLFNDPGFQKGDVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(3143..3598)
                     /gene="accB"
                     /locus_tag="ACFOA3_001118"
     CDS             complement(3143..3598)
                     /gene="accB"
                     /locus_tag="ACFOA3_001118"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6898132.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPVVTPAAAPIAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(3580..3843)
                     /locus_tag="ACFOA3_001119"
     CDS             complement(3580..3843)
                     /locus_tag="ACFOA3_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6898133.1"
                     /translation="MPRIPNRPPAYFRQKLLFFNILLDIYRQLRENKHIITVIFRRWK
                     NFSKPYSARFAPLPQVGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            3950..4990
                     /gene="queA"
                     /locus_tag="ACFOA3_001120"
     CDS             3950..4990
                     /gene="queA"
                     /locus_tag="ACFOA3_001120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6898134.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVGEGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMILGRNEGVAR"
     gene            complement(5362..5940)
                     /locus_tag="ACFOA3_001121"
     CDS             complement(5362..5940)
                     /locus_tag="ACFOA3_001121"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6898135.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            6178..7077
                     /gene="crgA"
                     /locus_tag="ACFOA3_001122"
     CDS             6178..7077
                     /gene="crgA"
                     /locus_tag="ACFOA3_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6898136.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLTVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVVASPEYLAKHGTPQSAEDLAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(7302..10517)
                     /gene="carB"
                     /locus_tag="ACFOA3_001123"
     CDS             complement(7302..10517)
                     /gene="carB"
                     /locus_tag="ACFOA3_001123"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6898137.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSNPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(10546..11187)
                     /locus_tag="ACFOA3_001124"
     CDS             complement(10546..11187)
                     /locus_tag="ACFOA3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFC6898138.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(11866..12255)
                     /locus_tag="ACFOA3_001125"
     CDS             complement(11866..12255)
                     /locus_tag="ACFOA3_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6898139.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRASRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(12828..12953)
                     /locus_tag="ACFOA3_001126"
                     /pseudo
     CDS             complement(12828..12953)
                     /locus_tag="ACFOA3_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(12999..14132)
                     /gene="carA"
                     /locus_tag="ACFOA3_001127"
     CDS             complement(12999..14132)
                     /gene="carA"
                     /locus_tag="ACFOA3_001127"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6898140.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMASGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         3144 a         3678 c         4057 g         3782 t
ORIGIN      
        1 gggaatgacg ggcgtgtata tttttgattt caatctgctg taaaaatgcc gtctgaaatg
       61 caggatttgc gcttcagacg gcattttttg tttcttattg cgcttcggct ctcggggcgg
      121 aaatcagaaa gtgttcgcgg tagtggcgca tttcttcgat gctctccaaa atgtcgtcca
      181 atgccttgtg cgaaccgcgt ttgacgacgc ttttggcaac gggtggattc cagcgtttgg
      241 cgagttcttt cagcgtggaa acgtcgaggt tgcggtagtg gaagtagttt tccagtttcg
      301 gcatatattt gaccatgaag cgccggtctt ggtggatgga gttgccgcac atcggcgtgg
      361 cgcgtccgga tacccattcc gacataaagt ccagcagttt ctgttcgact tcggcttcgg
      421 tatgcgacga ttcgcgcacg cgctgcgtca gccccgtcct accgtgtgtg gcggtgttcc
      481 actcgtccat attgtcgagc agctcgtcgc tttggtggat ggcatagact tcggattgcg
      541 ccaatacatt taaatccgag tcggtaataa tcatcgcgac ttcgataatg cggtcggttt
      601 cgggattcag ccccgtcatt tccatatcga gccagcagag gttgttttta tcctgcacgg
      661 tgtttccttt ccgtttcagc gttttacgcc gctcaatcgc gcccatcctt cttcggtttc
      721 cgcaggatcg agatcgaacc attggctgta aatgccgctg agttcttcga tttgttcgtc
      781 caacaaaccg gataacacga tgcggccgcc ttgtttggtg cgggcggcga gcatttcgcc
      841 gagcatacgt aaagggttgg cgaggatgtt ggcgacaact acgtcgaatt gcccttgagg
      901 cagaccgtcg ggcaggaaga attgtgcatc gacgttgttt tgcgcggcgt tgtccctacc
      961 ggcgcggacg gcctgttcgt caatatccac gccgacggcg gaacctgcac cgagtttgag
     1021 ggcggctatg gtcaggatac ctgaaccgca gccgtagtcg aggacgcttt cgccgttttt
     1081 gagttgtgta tccaaccatt tgaggcagag gcgcgtggtc gggtggctgc cggtgccgaa
     1141 ggctagtccg ggatcgaggc gtaggttgac ggcgctgcct tcgggggctt cgtgccaaga
     1201 gggggtaatc cacaggcgtt cggaaatttg gatggggtcg aattgcgctt gggtcaggcg
     1261 tacccagtct tggtcttcga gggtttcgcc ggtgtatgcc aagtctttta atccgcattc
     1321 ttgtgcggcg gtttggatga tggaggcggc ttcgtcgtgt tcgccgaaca gggcgatgac
     1381 tttgctctgc tgccagattt gttcggtcgg cataccgggt tcgccaaaga tggcttgttc
     1441 gttttcggtg cctgcatagg catcttcaat ggcggcggag agtgcgccgt gttccatcag
     1501 ggtgtcggcg aggcgttcgg cgacggcatc gttgacgttg atggtgattt gttggtaaga
     1561 catagcgggc tttcttggaa atatcgaaat cggtttcaga cggcgcgggt aagggaaatg
     1621 ccgtctgaac gcggacgggc ggcttcagac ggcatatcgg cggcggttta cttgtcctgt
     1681 ttggctttgc gatcttccag ccagtgttcc aaatagtgga tgctgacgtc gcctttttgg
     1741 aaacccgggt cgttgaacaa gtcgcggtgc agcggcgtat tggttttgat gccggtaatc
     1801 gccagctcgg caagggctac gcgcattttg gccatggctt ggtcgcggtc tttaccgacg
     1861 acgcagactt tgccgatcag gctgtcgtag taaggcggga tgcggtagcc ttggtaaatg
     1921 tggctgtcga cgcggatacc gaagccgccg ggcagatggc agctttcaat cagacccggg
     1981 ctggggatga agttgtacgg gtcttcggca ttgatgcggc actcgaaggc atgaccttcg
     2041 attttgatgt ctttctgctt gtattgcaac ggcaggccgg ccgcgatgcg caattgctct
     2101 tgtacgatgt ccacgccggt gatgagttcg gtaaccggat gctcgacctg aacgcgcgta
     2161 ttcatctcga taaagaaaaa ttcgccgtct tcgtataaaa attcaaacgt acccgcgccc
     2221 cggtagccga tgcgtttgca cgcgtcggca caggctttgc cgattttttt gcgtgcctct
     2281 tcatcgatga acggagccgg tgcttcctcg atgacttttt ggtggcggcg ctgcagcgaa
     2341 cagtcgcgtt cggcaaggta gatggcgttg ccgtgttcgt cggcaatcac ttggatttcg
     2401 acgtggcgcg gacgttgcag gtagcgttcc atataaacca tcgggttgcc gaatgccgcg
     2461 cctgcttctg ccttggtcat ttcgacagac tggaggaggt cttctttttt ctcgacgacg
     2521 cgcataccgc gtccaccgcc gccgcccgat gctttgataa tcacgggata accgactttg
     2581 tcggcgattt tgaggatttc ttcgccgtcg tcgggcagcg cgccgtcaga accggggacg
     2641 cagggtacgc ctgccgctat catcgcgtgt ttggcggaga ctttgtcgcc catcaggcgg
     2701 atggtgtcgg gtttcgggcc gataaaggta aagccggact gctcgacctg ttcggcgaaa
     2761 tcggcgtttt cggcaaggaa accgtagccc gggtggacag cgtccgcgca gcttacttcg
     2821 gcggcggcaa taatggcggg gacgttgaga tagctttgcg cggaggcggc agggccgatg
     2881 cacacggatt cgtcggcgag tttgacgtgc aggctgtctt tgtcggcctc ggaatgcacg
     2941 gcgacggtgg caatgcccat ttcgcggcag gcacggagta cgcgtaatgc gatttcgcct
     3001 cggttggcga ttaaaacttt tttcagcatg atgactgttc ctgtggttcc ggcaacccgt
     3061 ccgtaaaaaa aaaggtgcgg gaacgccgaa gggggaaagc atttcagacg gcatcggaag
     3121 tttatgccgt ctgaaaacag gattatccga tgatgaagag cggttcgccg aattcgacgg
     3181 gcgtaccgtt ttcgaccagg atttctttga ccgtgccgga tttttcggct tcgatttcgt
     3241 tcatcagctt catcgcttcg atgatgcaca gcgtgtcgcc ggctttaacc tgttggccga
     3301 cttcgacaaa aggcgcggca ttcgggccgg gtgcgcggta gaacgtgccg accataggtg
     3361 atttttgcgc gtcggacaaa tcgcgggcgg ccggtgcggc ggcgggcgcg gatgccgcaa
     3421 tgggtgcggc ggcaggcgtt acgaccggcg cggcggcagg tacgggcgcg gcgtaaacag
     3481 gtgcggcggc gatggtacgg gtaatgcgga ctttttcctc gccttcggtt acttcgattt
     3541 cggcgatacc tgattcttca accaaatcaa tcagtttttt taatttgcgc aaatccattt
     3601 ctgttccttt aaaggctgcc ggtgttccgg cgggttgttg cgttggtttt tcagaaaaca
     3661 gccgacttgc ggcaacggcg caaaccgggc ggaatagggt ttggaaaagt ttttccaacg
     3721 gcggaaaata accgtaataa tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa
     3781 aatattaaaa aacagcagtt tttggcggaa atatgcagga ggtcggtttg gtatgcgagg
     3841 tatgctttgt tgagagtttt tattctaatt tattggtttt tcggacaaag atgccgtctg
     3901 aaaaagataa gtgcgtataa tggccactgt tccgaatgaa agtgtcacaa tggatatctc
     3961 agattttgat tttaccctgc ctgaaaagct gattgcccag catccgcccg aagtgcgcgg
     4021 cagcagccgc cttttggttg cgttgccgga tatgcccctg caagaccggg tattcggcga
     4081 tttacccgat tatgtcggtg agggcgacgt tttggtgttc aacaacacca aagtcatgaa
     4141 ggcgcggctg tttgggcaga aagacagcgg cggcaggatc gaagccctga ttgagcgcgt
     4201 gttggacaac cataccgcac tggcgcacat ccgttcgtcc aaatcgccca agcccggtat
     4261 ggggctggtg tttgaaggcg gcatccgcgc cgtgatggtc gggcgtgagg gcgaactgtt
     4321 ctgcctgcgt tttgaaggcg gagagaccgt ttacgaactt ttggaacaga acggacacct
     4381 gcccttaccg ccttatatcg aacgtgccgc cgatgcggat gacgacagcc gttatcaaac
     4441 cgtttatgcc aaatatcagg gtgcggtcgc cgcgccgacg gcgggcctgc attttacgga
     4501 agaacttttg cgccgcctga aagacaaagg cgcagtaacc gcagaagtaa ccctgcacgt
     4561 cggtgcgggg acattccaac ccgtgcgtgt ggaaaaaatc gaagaacaca aaatgcacag
     4621 cgaatggttt gaagtgccgt ctgaaaccgc cgccgccgtt gaggcggcaa aagcccgggg
     4681 gaacaaagtc tgggcggtcg gcacgacttc catgcgcgcc ctcgagtctg ccgcgcgtgc
     4741 gacgggtcgt ctgaaagcgg ggcagggcga caccgatatt ttcatcacgc cgggctaccg
     4801 ttttaatgtt gccgacaggc tggttaccaa tttccatctg ccgaaatcga cgctgctgat
     4861 gcttgtcagc gcgttttcgg gtatgggtca tatccgcgcc gcgtatcgtt atgcggtcga
     4921 acgcgaatac cgtttcttca gctacggcga tgcgatgatt ttggggcgga atgaaggggt
     4981 ggcacgttag aacagcctca agtctattgg gggaagacaa gtgccgtctg aacggacgtt
     5041 cggacggcat tcttttgttg atttgaatga aaggaactgt atatgacata gattcaggcg
     5101 cgagaggcga gaaactcagt cattgttgat gacgcgcaat acgttaaaat tcattccgtt
     5161 ttggtcgtat aaacatatta catacgccat ttttttgtat tcaaggctgc ctgaaaacag
     5221 tgggcaagaa tatctaaccc aacgaaaagc tgaaaaagca acaaaaaata atctgcacct
     5281 tagtcggttg gttagtccaa ccttaggggt gcagaccatt caatctttca ggctgccttt
     5341 gtttgtttat aagataaatt tttagccgaa cactttttct aagtgtgcct gataatctgc
     5401 caagtatttt tccacttgcg gatttttaac cacatcgtta cataagaatg tcggcagacg
     5461 ggtcataccc aaaaactcgt tggctttgtg gaagtgcata tacaaaacat caacgccttt
     5521 gccttcaaag aaatcgcctt cgcgggtaaa cgcctcaatc ggcgcattcc aagtcagtga
     5581 aagcatatgt tttttgcctt gcaacaagcc gcctgtgccg tagccctcag tcggattgac
     5641 gcggtgtctg ccgtcgcttt ggtagagttt gccgtgtcca gcggttaata cttcgtctat
     5701 gtattttttc actgtccaag gctcgtgcat ccaccagccc ggcatctgcc aaatcacggc
     5761 atccatccaa acgaattttt cgatttctgc ctcaacatca tagccggcat caatcacggt
     5821 ttcttgtaca ttgtgtccga gcgcggtcaa aacttctttc gcttttttgt gaagcgtgtg
     5881 gtttaacccg ccgtgagaat gtccgaacgc tttgccgccg tctaataata aaatattcat
     5941 ttgttacctc ttttgtgaat tgatgaatgt attgtgccgt tttactttat aatttaaaag
     6001 tgcaaaaata agaaaacact tttgcgtcaa atggaataat cagatgtttc aacacacggg
     6061 atgacacata aagcgttgcc ctatgtatcg tcctgatttt gaaggggtac gcccctccca
     6121 aataaagtct gatcctgccg ccctaaaggg cggggtttca accgaaaagg aaacacgatg
     6181 aaaaccaatt cagaagaact gaccgtattt gttcaagtgg tagaaagcgg cagcttcagc
     6241 cgtgcggcgg agcagttggc gatggcaaat tctgccgtaa gccgcattgt caaacggctg
     6301 gaggaaaagt tgggcgtgaa cctgctcaac cgcaccacgc ggcaactcag tctgacggaa
     6361 gaaggcgcgc aatatttccg ccgcgcgcag agaatcctgc aggaaatggc agcggcggaa
     6421 accgaaatgc tgacagtgca cgaagtgccg caaggcgtgt tgcgcgtgga ttccgcgatg
     6481 ccgatggtgc tgcatctgct ggcgccgctg gcagcaaaat tcaacgaacg ctatccgcat
     6541 atccgacttt cgctcgtttc ttccgaaggc tatatcaatc tgattgaacg caaagtcgat
     6601 attgccttac gggctggaga attggacgat tccgggctgc gtgcacgcca tctgtttgac
     6661 agccgctttc gagtagtcgc cagtcctgaa tacctagcaa aacacggcac gccgcaatct
     6721 gcagaagatc ttgccggcca ccaatgttta ggctttaccg aacccggttc tctaaataca
     6781 tgggcggttt tagatgcgca aggaaatccc tataaaattt caccgcactt taccgccagc
     6841 agcggtgaaa tcttacgctc gttgtgcctt tcaggttgcg gtattgcttg cttatcagat
     6901 tttttggttg acaacgacat cgctgaagga aagttaattc ccctgctcgc cgaacaaacc
     6961 tccgataaaa cacacccctt taatgctgtt tattacagcg ataaagccgt caatctccgc
     7021 ttacgcgtat ttttggattt tttagtggag gaactgggaa acaatctctg tggataattc
     7081 tataaaaacc tgccataaaa ttgtttttac tcacttaata actttgagaa aaaataaata
     7141 agatgatgta tagccgcggc aatggcagtc tatatgggga gcgtgatagg gctgttcctt
     7201 atgtacggct tgtgtgtggc aacatcggct ttccaaatga caaaatgccg tctgaaaccg
     7261 caaaacggct tcagacggca ttttccaagc tgattcaggc atcagttgcg gtttttcaaa
     7321 cgcccgtgca gttcttgaac gctgtacacg cccagatggt ctcggctttt cgcgccttcg
     7381 ctcatcgctt cgccgccggc ggtggtggtg tattgcggca cacgttgctg caatgcgctt
     7441 tggcggatga tgtggctgtc ggacacggat tgcggattgc tggaaacggt gttcacgacc
     7501 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcgaccttc ggggactttg
     7561 ttgatggtct gcacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgtgtggcg
     7621 cagatgccgt agcctaatgc ttggaaattt ttggcggttt tgatgacgcg ttctttgtct
     7681 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc cgcgccgagt
     7741 tgggctttgt agtaggcttc gccgaagctt gcgcccacgc ccatcacttc gccggtggag
     7801 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggcc
     7861 tctttaacgg catagaaatc ggggacgact tctttttcca cgccttgttc tttcagggaa
     7921 atgcctgcca tacagcgtgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     7981 aagggcacgg tacggctggc gcgcgggttc acttccaata cgaacaccac gccgtcctgc
     8041 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat cgctttggtt
     8101 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagccg
     8161 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccaca
     8221 tctttgccgt ctgaaacgca gtccacatcg acttcaatcg cgttgttcag gaagaagtcg
     8281 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaaggct
     8341 tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     8401 agcggatagc cgatttcttc ggctttgacg agggcttctt cttcgttgtg tgcgatgcgg
     8461 ttgggcggtt ggcgcaggcc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     8521 gcggcatcga tgctgtcggc ggatgtgccg atgatgttca cgccgttttc aaccagcgcg
     8581 ttggcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     8641 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     8701 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccataatcgt ttcaaaaccg
     8761 gattcgcgca gggcgagcgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     8821 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     8881 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     8941 gtgtcaacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgcacttct
     9001 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     9061 cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttcc
     9121 ttcatcaagt cttcgatttg cgccaggaac caagggtcga tggcgcagat ttcgtggatt
     9181 tcttccagcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
     9241 gggtttgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt gaagccgcac
     9301 aagcctgttt ccaagccgcg cagggctttt tggaagcttt cctgaatcgt gcggcccatc
     9361 gccatcactt cgcccacgga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     9421 ttttcaaacg caaaacgcgg gattttggtt accacatagt cgatggacgg ctcgaatgac
     9481 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcttaaa gccgaccgcc
     9541 agtttcgccg ccaccttcgc aatcgggaaa cccgtcgcct tggaagccag cgcggacgaa
     9601 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     9661 aactgaacgt tcgagccgcc cgtgtccacg ccgatttcgc gcaataccgc cagcgaagcg
     9721 ttgcgcatga tttggtattc tttgtccgtc aacgtttgcg ccggcgcaac cgtaatcgag
     9781 tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcagatgat gatgcagttg
     9841 tcgtttttat cgcgtacgac ttccatctcg tactctttcc agccaaggac ggactgctca
     9901 atcagcagct catgcgtggg cgaggcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     9961 tctttattgt aggcaatacc gccgcccgaa ccgcccatcg tgaaagaagg acgaatcagc
    10021 gtcgggaagc cgacctgctc ctgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
    10081 gatttcgggc aagacaaacc gattttttcc atcgcttctt taaagcggcc gcggtcttcc
    10141 gccttgtcga tcgcgtcttc cgtcgcgcca atcagctcga cattgtattt tgccagcacg
    10201 ccgttgcgtg ccaaatccag cgcacagttc agcgcggtct gaccgcccat cgtgggcaga
    10261 atcgcatcag gccgctcctt ggcgataatc ttttccaccg tctgccacat aatcggctcg
    10321 atgtaggtaa catccgccat ttcagggtcg gtcatgatcg tggcgggatt ggaattcacc
    10381 agaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc cgaatagtca
    10441 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
    10501 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
    10561 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
    10621 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
    10681 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
    10741 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
    10801 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
    10861 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
    10921 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
    10981 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
    11041 tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt gattataatt
    11101 aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca aatactttaa
    11161 attattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt gcaataaacg
    11221 tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct aaaatgttgg
    11281 gtttgctaac ctaacttgta tatcgaaacc cccgtattgt agcaagccgt agcccgtatg
    11341 tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc caacccaccg
    11401 cgtgcgtgcc ttgcggcaca ctctctgccg atgggtttaa aggtcgtctg aaaaaacgct
    11461 cgcggcgttt gttccatctg ccttgcgata aaggctgcct gaaaaattcc cgctgttgat
    11521 atttcgggca ttctttctca aaccgcctgc aaccgcaaaa tccgctgcaa tctgccccct
    11581 cccccgtggg ggaggaccgg ggagagggca ttcttcgaat ttcggcaacc ttccccaaca
    11641 ccctaaccgc tcaaacacaa gccttgcggc ttgttgccct ctctctggct ctctcccacg
    11701 ggagagagga cgtggtggct gttggaatta agggttctgc aggaaaaaca ggttgtgcgg
    11761 gctgtttttt gtttttcaga cggtcttcta aacgctcaat tcaagcagcc tgtaccctac
    11821 ctgtatccta taattttcaa aactctaatc aaaattaacc gtttgttatt gcgcatactg
    11881 cttttccaat tcctgcaata cgcgcaggat ttccgccagt acatcgtttg tctgctgcaa
    11941 aatttcgtga ttccaaaaac gcagcacggt aaagcccagg ctgttgagat atgccgtccg
    12001 cgcgtggtcg tatacggctt gttccgcgtg ctgcccgccg tctgcttcga caatcagctt
    12061 gggcgttacg cacataaaat caacaatata gtttcccatt ggctgctggc ggcggaattt
    12121 gtagccattt agacggcttg cccgcaggtg ttgccacaat tttgcttctg cctcgctcat
    12181 ttcttggcgc atggctttgg cgcgttggcg caagatgggg ttgtcggtgg tcaagagttt
    12241 ctcgtgcggg ttcattttgt tgtcgtgctg tttgctgccc tctctctaac tctctcccac
    12301 ggggagagag gacggggcgg ttgttggggt taaaggggct gtaaggaaaa caagtcgttc
    12361 aggccacttt ttacttttca gacgacctgc aatcaacggt tgtaaatcca agttgctttt
    12421 caaaccgcct gtaaccgcaa aacttgctgc aacctacccc ctcccccgtg ggggagggct
    12481 ggggagaggg cattcttcga atttcggcaa ccttccccaa caccctagcc gcccaaacac
    12541 aagccttgct gtttgttgcc ctctctctaa cgctttccca cagggagaca ggacggggcg
    12601 gctgtagtga ttaagggttt tgcaaggaaa acaaggaaaa caagttgtac aggctgtttt
    12661 ttatttttca agcgacctga aatcaacgct tgtaaatcca agtcgctttt caaactgcct
    12721 gcaaccgcaa aatctgctgc aacctacccc ctcccccgtg ggggagggct ggggagaggg
    12781 cattttccaa gttgcggcaa tctttcccaa atcccttagt cttctaaaat acaagcctta
    12841 cggttgttgc caaatgaaac ttcttccata cccaaaactt gcgtgtaaaa cgcaatggtt
    12901 cggtcaatgt cggcaacggt cagtaccaga tggtcgagtg cgctaatctt catttcccgc
    12961 tttctttttc aggcggcggc agtgccgtct gaaagccatt atgcccgttt tgccgctttc
    13021 atattgccaa tgaatttgtc aaacaaatag ccgacatctt gcggaccggg gctggcttcg
    13081 gggtggcctt ggaagcagaa cacaggtttg tcggtcagct cgatgccttg caaagtgttg
    13141 tcaaacaagg atttgtgggt aatgcgtgcg ttagcgggca gggtgtcggc atcaacggca
    13201 aaaccgtggt tttggctggt gatgacgact ttgccgctgt ccaaatcttg cacagggtgg
    13261 ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatagc gaggctgatg
    13321 agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc ttgccatcag tttttgcacg
    13381 gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag gaacacgccg
    13441 tcgggattga gtgccaacac gtcttgtgcg ctcgtttggg cggggacgac ggtcaggcgg
    13501 cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc gtaggcgacg
    13561 acgtggtaag gctgttcgtc aggggtaacg aaacccttgc ccaatgccca ttcgccttcc
    13621 gtccattcgt aagtttccgt gcaggaaact tcttttgcca agtctttgcc gaccatactg
    13681 ccgaacgcgg cgatgagttc ttgcgctttt tcgattgtgg catccgcgcc ggtcagaatc
    13741 gcgccgcctt gcgcgccttt ttcgcgcaac agcgtggtca ggcggcgggt atcgatgtcg
    13801 gcgatggcga cggtttggtt gcgtaccaga tagtcgtgca ggctttcgga ggcgcggaag
    13861 ttgctgtgca agagcggcag gtcgcggata atcaggccgg cggcataaac gctgcggctt
    13921 tcttcatctt cggcgttggt gccggtgttg ccgatgtgtg ggtaggtgag ggtaacgatt
    13981 tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cggtcatcga agtattgaac
    14041 acgacttcgc cggaagtcga accttcgtaa ccgattgatg tgccgtggaa tacgctgccg
    14101 tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt aataaaattc
    14161 ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa atccggtcaa
    14221 aaaaaacacg ccgcgcaaga tagacgcgta acgtgctttt cgggcaagtg cctataccct
    14281 gaaagcagca tattttaaga cgaaacatac ccgatttcaa gcgcaaacgc ggcaggcggg
    14341 ctgatatgtt cgcgcgaaaa cggaaatccg tggcgtaaaa ccgcccgctc cgcgcccact
    14401 atgcccgtta cgcatttccc cacgtccgtc cggcgaaact atgggaatac ccgaatcgtc
    14461 aggaatacct gaatcgtcag gaatacccga atcgtcagga atacccgaat cgtcaggaat
    14521 acccgaacca tcattcccgc gcaggcggga atccggactt gtcggcacgg aaacttatcg
    14581 ggtacaacgg tttcttgaga ttttacgtcc tagattcccg cctgtgcggg aatgacggca
    14641 tcggtttgtc ggcgtttcat t
//
LOCUS       DAXIEB010000047        14386 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000047 DAXIEB010000000
VERSION     DAXIEB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.047"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(121..4290)
                     /locus_tag="ACFOA3_001128"
     CDS             complement(121..4290)
                     /locus_tag="ACFOA3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6898141.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLSWLAGTESGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISVGKAFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFVLDTAIYTKGGLKGETIHSTARLSGSLKDVRAELAIDGGNIRLSGKSIIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFVDRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIKGPTASPKISWQLDTGTARTDGSLAIASDPAN
                     GQRKLVFDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTAGLMLDGTGAQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNTDAH
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEAFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLILDLN
                     DSIRFAGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSLSSGGELTYTIRFDRLFSSD
                     KKDSAGNSKGK"
     gene            complement(4345..6192)
                     /locus_tag="ACFOA3_001129"
     CDS             complement(4345..6192)
                     /locus_tag="ACFOA3_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6898142.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAVVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLAHFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGISLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6603..7832
                     /gene="sstT"
                     /locus_tag="ACFOA3_001130"
     CDS             6603..7832
                     /gene="sstT"
                     /locus_tag="ACFOA3_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6898143.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            7949..9427
                     /locus_tag="ACFOA3_001131"
     CDS             7949..9427
                     /locus_tag="ACFOA3_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6898144.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEAKEDEPQAGSQGQGAPSAQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSADTDSLTPNHTPVPNMPTVDMENQAPDSGESAQPENQPDVANAADGMQGDDPSA
                     GGENAGNTAAQGTNQAENNQTAGSQNPASSTNPSATNSGGDFGRTNVGNSVVIDGPSQ
                     NITLTHCKGDSCSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDGKNDKFVGLVA
                     DSVQMKGINQYIIFYKPKPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVS
                     LTGHSGNIFAPEGNYRYLTYGAEKLPGGSYALRVQGEPSKGEMLAGTAVYNGEVLHFH
                     TENGRPSPSRGRFAAKVDFGSKSVDGIIDSGDGLHMGTQKFKAAIDGNGFKGTWTENG
                     GGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9502..9717)
                     /locus_tag="ACFOA3_001132"
     CDS             complement(9502..9717)
                     /locus_tag="ACFOA3_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898145.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9710..9925)
                     /locus_tag="ACFOA3_001133"
     CDS             complement(9710..9925)
                     /locus_tag="ACFOA3_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6898146.1"
                     /translation="MKEYKVIIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10000..11343)
                     /gene="argG"
                     /locus_tag="ACFOA3_001134"
     CDS             complement(10000..11343)
                     /gene="argG"
                     /locus_tag="ACFOA3_001134"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6898147.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11670..11810
                     /locus_tag="ACFOA3_001135"
     CDS             11670..11810
                     /locus_tag="ACFOA3_001135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898148.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11807..12607
                     /locus_tag="ACFOA3_001136"
     CDS             11807..12607
                     /locus_tag="ACFOA3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6898149.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12647..13633
                     /locus_tag="ACFOA3_001137"
     CDS             12647..13633
                     /locus_tag="ACFOA3_001137"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6898150.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14290..14386
                     /locus_tag="ACFOA3_001138"
                     /pseudo
     CDS             14290..14386
                     /locus_tag="ACFOA3_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3031 a         3686 c         4329 g         3340 t
ORIGIN      
        1 tcctgatttt tgttaattta ctatatcgat tctgttccct tgcaggcgga cggcggcggc
       61 ggacggtgga aaagcaggtt ttcaaatgtc cggtttggcg gcgcgccgtc tgaaaaccgc
      121 ttattttcct ttgctgtttc cggcggagtc ttttttgtcc gaactgaaga ggcggtcgaa
      181 acgtatggtg tatgtcagct cgccgcccga cgaaaggctg ccgatacggg caaccgcctg
      241 tatagcgcgg gtcagccggt aaatcagttt gacggactgt tccgcgctgg agatgccgta
      301 ttcgtagccg atgtagagtt tgccggtcag ttgtttgccg acggtcagca cctgttcggc
      361 ggggttgagt tcgccggttt gcgcgttgcg gctgcgcttg ctggtaaagc ccaaatcatc
      421 caccagcccg atgcggtcgt tgatttgtcc ggcaagcagc gcgcctgcgg ctgcggacag
      481 ggcagcattg tcgccgctgc tgccgctgcc ggcgcggttg aggatgagcc aagagagctt
      541 gtctttttca ctcatcggtt cgtttgccgt cagtgtaatg cgcgggctgt tgaggctgcc
      601 caatatttcc acgcccgcac cgacggggga aaggcggcgt tcggcgcgga tattgaggtt
      661 ggggtcgttg agcgggccga caaaggagac cgtgcctttg gtaatgtcca aatcctgccc
      721 gtatgccttg taacgccctt tgatgacacg gaccgtgccc acgccccgca cgtttccgcc
      781 cggttgcgcg gtcagggtca gtttgccgcc tatggtaaca tccgcgccgt agccggcgaa
      841 gcggatgctg tcattgaggt ctaaaatcag gttcatattg acggggagtg gtgccgccgc
      901 ctcttttttg acttcgccca atacgacgac atcgtcgccg acggacggca tcgaggattt
      961 ttgcgaaccg aacagcccct gatccgtttt aatcatcccg gtaacggata tgcctttttg
     1021 cggcgaatag cgcaggcgag tgttgccgga aaccgtcagg cggcggttgg ggcgggacag
     1081 gatgcggtat ttgtcgaaca ccgcgccgat atcgacatcg ggcccgctgt tttccatacc
     1141 gaccgtgccg gagagttccg ccgttccttc gtgccggaat ttcaggctgt cgattaccca
     1201 tttcctgccc gcgatatgcg aacgcagcga gccgttgtcc aagatgatgc cttgggtttg
     1261 gttgcggtaa tagagtttgt cgccgttgat gctgccgccc aagtgcggat cggcgatgct
     1321 gccaccgagg gttacggcgg cattcaggct gcctttgacg gtttgtccga cgggcaggaa
     1381 gttgcggaat gcttcggcat cggcaacggt caggttgagc ctgccgccca aaggcgcggt
     1441 atcgaaggag cggctttgcc cgacggtgat gttgccgttg attttcccgt agttgctgct
     1501 accgttgaca gcggcgttga cggacggaga gccgacccgt ccgccgatat gcgcgtcggt
     1561 gttcaggctg ccggtaatgt tttgcgcggc ggcgggcaga aagggcttca atgcgcccaa
     1621 gtcgggaagg gaggcggtaa tcctgccgcc gagcggtgcg tttgccatat tgccgccgaa
     1681 ggcgttgccg atgcccaaat cggcgttaat ccgtccgaaa cgcgcaccgc cgtcaagcag
     1741 gattccgatg cggtcgtttt gaaagcgcgt tttcagggaa aatgcgttca aacccaaagc
     1801 ctgcccgccg ggcaatacgg catcgccgct ttgtcggctg atattgaggt agccgcgcgc
     1861 gttgcgcccg taggcgacat cccagtcgcc gtttaaaacc agattgtgtt cgaagggcgg
     1921 tttgaagaaa ttgtgcaact cggcgatatg caggccgtgt gcgccgcctt ttgccgatat
     1981 gccggttttt ttatcccaag aaaagtgttg caggttgagg ctgccgccca ttgcctgcca
     2041 atttgccgca cttgccgcca cgcgttccgc accggcttcg agcgtcatac ggttttgcag
     2101 cttgaggttg aacgcgccgc cgatatcgag gatgccgatg ctgcctttcc atagggtaag
     2161 ttccctgttg atgccgcctg aagcgtccaa atcgaatttg aacggtttgc cgtccagcgt
     2221 catggcggcg tgtgtgcgga tgcggtgctg cgcgcccgtg ccgtccagca tcaggccggc
     2281 ggtatcgaca accgccgccc cgcccgacag cgaaaggcgg ctgcctttga tgtcggcgcg
     2341 tatcgggcgg cttgtgtcgg gcgaaccttt gagcgtgaaa tcgagcgaac ggatgtctgc
     2401 cgccttgccg atgtgcaggt tgcgcgccgc gccggaaagg tcggtttcaa aggttcggat
     2461 gccgccgtcc aaatcgccgg aaaggtgtcc gcgtacattt aaagaccccg cgagtccgaa
     2521 accgaaacgg gataaatcgg gtgcggtgat attgaggtta agccggtcgc cttttttgcc
     2581 gaagccgccg tctgttttga cgatgttccg ccccagccgc aaatcgacgg cggcgcgcgg
     2641 aaggtggcgg gactcgtaaa caatgtcggc actgccggca atcggtacgc cgttgaacgt
     2701 gccgggtaaa aaccgcattt tgcctgtgaa tttctctttt gccagttcgc cggcaaggtt
     2761 tattgagccg ttgatattgc ctgccggaag ttgcggatcg atgcgcgaag ggtcgaatgc
     2821 gcgggaacgg atgtccagct tgagcaggcg gtctttaaac agctcgagat agccttgcgc
     2881 ggtcaggctg ccttgcccgg cggcgatgtt gacggtgtcg aataccagtt tccgctgtcc
     2941 gtttgcgggg tcgcttgcaa tggcgaggct gccgtccgtg cgtgccgtac cggtgtcgag
     3001 ttgccaagag attttgggcg aggcggttgg gcctttgatg ccgatgctgc cgtccaacct
     3061 gcctttgaac gcggtttgca gcacgtcttc tgcgccgacg gagttgatgc ctatatttaa
     3121 atcgaggatg tctttttcgg tgtcgatttt gcccgacagc ctgatgccgc cccgtccgag
     3181 cagggcggcg gacgtattgc cgatatgcac cgtgccgtcc tgccggatga caaagccgcc
     3241 caaaacctga cggacgggga tgccgttgcg gtcgacaaag ccggctttgg tgttttccaa
     3301 atcgagcgag ccttccagcg cgatgccgtc tgaaaacgac gggatggcgg tcaggtcgaa
     3361 attcagtccg gcatcgggca gggaaggcac gaaggcggac ggattgatgt tgaatccttt
     3421 gaccagcact tcttccaatg ttttatccaa tgattcggca aacgggtgga tgatggattt
     3481 tcccgagagg cggatattgc cgccgtcgat cgccagttcg gcgcgcacat ccttcaggct
     3541 gccgctcagc cgcgccgtac tgtgtatggt ttcgcctttg agtccgcctt tggtgtaaat
     3601 ggcggtatcg aggacaaacg gttttttcaa gccgaccgag gcagcccccg acgaactgct
     3661 ccacggcgtg tcggcggcct tcaggtcgag gcggtgtcct ttgcggtcgt aacggtatgc
     3721 cgcgttgagc cgttcgagat agacggtttg tttgtcaaag gctttgccga cgctgatttt
     3781 gcccgtctcg aagcggtcga gatagacggc ggcaggcagg tctatgctgt cgggaaggct
     3841 gagcggaggg cgttcttctt taggcggagt cggtttggta acgatggcga tgtcgccggc
     3901 ggaaatttcg gtgatgtgca ggctgcggcg catcagttcg gacggtttcc acgcgaagcg
     3961 gaagcggctg attttaaggt ctgccccctc ggtttctatc gaccagttgt cgccgtcgaa
     4021 gccgtcgagc agcgtgcctt tgaggttttg ggaggaaatg tttacgccga accaagacgg
     4081 gatttggtac agcccgaaac gcaaaccgga ttccgtaccg gcgagccagc tgaggaaaca
     4141 tactgccaaa atcaggacag acagcagtgc cgccgacagc ttcagcagcg ggcggcgttt
     4201 ttttgccggc ggggcagggc ggtgttcaga cggcattttg cgcgtgccgt tttcggtcgg
     4261 atcggtatct gtcggtgcgg tatcggtcat aatgtctgtt tcaaaatagt ttgctgcaat
     4321 gccgtctgaa gcggccgtat cggtttagaa gcgcgttccc aagctgatgt gccagcggat
     4381 tttcttatcg ctgtgcccgt aggcgatgtc gaaggaaaac ggcgcgagcg ggctgaacca
     4441 gcgcacgccc agtcccgaac cgtgtttcag cttcatacgt ttgaaattgg cggcggcgtc
     4501 gcccatatcg tggaacaccg cgccggaaag ggtgcgcgta aacggcagtt ggtattccag
     4561 gctgcccacc aagagggcgc gttcgggcag gaccgatccg ttcgggccgg caagcccgat
     4621 gctgtcgagt tcgtaaccgc gcacggaaga cgcgccgccg ctgcggaaca tcagccccga
     4681 aggaacgtcg gcattgtcgc gggcaacggt gtaacccgct tgtccgcgta tgatgaacgt
     4741 gccgagtttt ttgttttcgg gcgtgaagaa ataacctgcg cgggcagagg tgcggatcag
     4801 cgcggtggag gacaggaatg tgcccaaagt cgtaccgatt ttgccgtcga ggtaatggcc
     4861 gttttcggga tgcagcacgt tgttgagcag ctggcgtttc caagaggcgg tcagcatcgt
     4921 ggcgtggctg ttgcccaaat cgacatccga gccggggatt ttccggcctt ctgcgagaaa
     4981 ctccgccccc agcctggcat caatgcccgc gcggtcgcgc acataccaga tgccgccgga
     5041 gaaggcgcgt ttttcgaggt tttgggtggt cgaacggttg taggagacgt tgcttgtcca
     5101 gtagttgccc ctgtagttgc ggggttggct gatgccggcg gcaagcgtgg tttcgtattt
     5161 gtccatatcc cagacaaccg aaccgatata gcctttgttg aagaggttgt aatagtcgta
     5221 ggcgattttg ccgcccaaac cgtattccga atcgaggctg atgccggttt cgagtttgtg
     5281 gcgtttgacc tcggttacgc tgactttgac ggggacgcgg tcgccttgga ggcggtcgaa
     5341 gtcggcttgt acggacgcgc cggaataatg cccgttttgt tcgagcgcct gttggaagtc
     5401 gagcagcagg tcgaggtcgt agggcgtgcc cggttggaag tgcgccagac cggagacgat
     5461 ttgttcgggg taacgctgtg tgccggtgat ttcaaagtcg ccgaaggcga tggggcggcc
     5521 gctgtccacg acgacgttca aatcggcggt ggcggtatcg gggttgacga ccgcccgggt
     5581 gttgccgagc ttggcaagcg ggtagccttt gcgcgttacc gcgccgagga cggaagtttt
     5641 gctgttttcc caactgtcct gatcgaaatc gctgcctacc ggctgctgcc agttttccag
     5701 cgcgttgcgg tagtattcgg cgaggttgcc gtctgaaagg atgtcgccga ggatggcgac
     5761 gccgacgttg gcgattttgg tgcgcgggcc cggtgtgatg tgcaccgtat aagctccgtc
     5821 tttttccgtc aggctgactt tgctgctgaa atagcctttg ctgcggagca tcgttttaac
     5881 gttgtccggc gcttcttcgg cgaggaagcc cgtctgttcc ttgtccaata cttcttcctg
     5941 ctgctgcgtg atgagcggca ggtgttcttc gaccatatct ttgatttcac tgtcctgcgt
     6001 gtcgatgcgg acgggaaatt tgggttttaa tttgactgat tcggtgtcgg ggcttttgtt
     6061 tttgaacaat gcgaaacccg ccgccttgtt ttcggaaagg tcggcggcag gcgcgtatgc
     6121 gtgcggaaag aaaaataaag ccggcaggag cagggcggtc ggtttgatca tcatggtacg
     6181 ggtgtcgtgc atcggtgtgg cggttttcga ttctaacact gttcggacgg ggtttcgaac
     6241 ggctgttttt gtgttgttcg gacggggcgg gacgcttcag acggcatact gccgccggta
     6301 tttcggaggt tgcggcccgc cggaaatgta tctgcttgtt ttaaggtatt ttgtttccaa
     6361 tccgcatatg gaaaacaggc tgcgtttcgg aatagtgtcg aaaaataggc aggattaaat
     6421 aaatttattc tcgttcaata aattaaaccg tcgcgccgaa tgataaagca aagcctgtcc
     6481 ggcactgcat agggcaaatc aacgcaacct taaaacacag ccgaaaaata aaaaaaacac
     6541 agagaatgag ttttctttta aaatacaaca ttttttaaca tatcacaaca taaggaagaa
     6601 ttatggcgtt cggcaaatcc ttatttcatg cgatcggccg cgtcagcctg gtcaggcaga
     6661 ttgccgccgg tttggcgttg ggcatcgtaa tcggttcggt ttcaccgcaa ctgggcttgg
     6721 cggcaggctt gttcggcagc ctgtttgtcg gcgcgctcaa agcggtcgcg ccggttttgg
     6781 tatttatttt ggtggcagcc acaatcgcgc agcaccaaaa aggcaacaag gcgcatatca
     6841 ggccgattat cgtcctctac ctcatcggta cgttttccgc agccctgacc gccgtcatcg
     6901 ccggtatggt tttcccgacg cacattgttt tggcgggcgc gggcgatgtg tccgccgctc
     6961 cgccttccgg cattgtggaa gtattgaaat cgctgctgat gaacctggtc gccaacccga
     7021 ttaacgcgat tgccaatgcc aactatatcg gcattttggc ttgggctttg gttttgggcg
     7081 cggcgttgcg gaatcacggt tcggacgtta cgcggcaggt cgttgccgat ttggcggaag
     7141 cggtttccac cgtcgtgaaa tggattatcc gttttgcccc tttgggcatt ttcgggctgg
     7201 tgtcatccac aatcgcggag acgggtttcg gcgcgctggc gggttacgcg aagctgcttg
     7261 cggtgctgtt gggctgtatg gcgtttatcg cgctggcggt caatcccgcc atcgtgtggt
     7321 ggaaaatccg ccgcaaccct tatccgctgg tgtttacctg cctgcgcgaa agcggcgtgt
     7381 atgccttctt tacccgttct tccgccgcca atatccccgt gaatatggct ttggcgaaaa
     7441 aactggggct gcacgaagac acttattcga tttccatccc gttgggggcg accgtcaata
     7501 tgggcggcgc ggcgattacg attaccgttt tggctatggc ggcggcgcac acccaaggca
     7561 tacaggttga ttttgccacc gcgctgctgt tgagcctggt ggcgacggtc agcgcgtgcg
     7621 gcgcgtccgg cgtggcaggc ggttcgctgc tgctgattcc gctggcgtgc agcctgttcg
     7681 gcatcagcaa cgatgtcgct atgcaggtgg ttgccgtcgg cttcatcatc ggcgtgattc
     7741 aggattcggc ggaaacggcg ttgaattctt caaccgacgt tttgtttacc gctgccgccg
     7801 atttgggccg acagagaaac cgggcggaat aaaccaagct atgccgtctg aagcccgttt
     7861 ggcgttcaga cggcatattt ttacaatttt atgggcatat ggcggaaaca atgccggctt
     7921 tgctttattg aatacgaagg agatgatgat gtttaaacgc agtgtgattg caatggcttg
     7981 tatttttgcc ctttcagcct gcggtggcgg cggtggcgga tcgcccgatg ttaagtcggc
     8041 ggacacgccg tcaaaaccgg ccgctcctgt tgttgctgaa aaagagacag aggcaaagga
     8101 agatgagcca caggcaggtt ctcaaggaca gggcgcgcca tccgcacaag gcagccaaga
     8161 tatggcggca gtttcggcag aaaatacagg caatggcggt gcggcaacaa cggacaaacc
     8221 caaaaatgaa gacgagggac cgcaaaatga tatgccgcaa aattccgccg atacagatag
     8281 tttgacaccg aatcacaccc cggtaccgaa tatgccaacc gtagatatgg aaaaccaagc
     8341 accggattcc ggggaatcgg cacaaccgga aaaccaaccg gatgtggcaa atgcggcgga
     8401 cggaatgcag ggtgacgatc cgtcggcagg cggggaaaat gccggcaata cggctgccca
     8461 aggtacaaat caagccgaaa acaatcaaac cgccggttct caaaatcctg cctcttcaac
     8521 caatcctagc gccacgaata gcggtggtga ttttggaagg acgaacgtgg gcaattctgt
     8581 tgtgattgac gggccgtcgc aaaatataac gttgacccac tgtaaaggcg attcttgtag
     8641 tggcaataat ttcttggatg aagaagtaca gctaaaatca gaatttgaaa aattaagtga
     8701 tgcagacaaa ataagtaatt acaagaaaga tgggaagaat gacgggaaga atgataaatt
     8761 tgtcggtttg gttgccgata gtgtgcagat gaagggaatc aatcaatata ttatctttta
     8821 taaacctaaa cccacttcat ttgcgcgatt taggcgttct gcacggtcga ggcggtcgct
     8881 tccggccgag atgccgctga ttcccgtcaa tcaggcggat acgctgattg tcgatgggga
     8941 agcggtcagc ctgacggggc attccggcaa tatcttcgcg cccgaaggga attaccggta
     9001 tctgacttac ggggcggaaa aattgcccgg cggatcgtat gccctccgtg ttcaaggcga
     9061 accttcaaaa ggcgaaatgc tcgcgggcac ggcagtgtac aacggcgaag tgctgcattt
     9121 tcatacggaa aacggccgtc cgtccccgtc cagaggcagg tttgccgcaa aagtcgattt
     9181 cggcagcaag tctgtggacg gcattatcga cagcggcgat ggtttgcata tgggtacgca
     9241 aaaattcaaa gccgccatcg atggaaacgg ctttaagggg acttggacgg aaaatggcgg
     9301 cggggatgtt tccggaaggt tttacggccc ggccggtgag gaagtggcgg gaaaatacag
     9361 ctatcgccca acagatgcgg aaaagggcgg attcggcgtg tttgccggca aaaaagagca
     9421 ggattgatgt tgatgccgtc tgaagcgggg gcgttcagac ggcattgtga tgtttggggt
     9481 ggggtccggt tcaagtccgc atcagttgat aaacccgtcg tctttcattt tcttttcgga
     9541 aaaatactgg ccgcatacca gcagggcggc gaggatgatg acaaacagcc ccgccatagt
     9601 cagtgtgatt ttgaagaaca attccgcttc gagaaaagaa aaccaaagtt gaacaatcag
     9661 cagcagcacc cagccggcaa acagtccgag acaggcataa acgccgagct taaacacgat
     9721 cccgctccaa aatgacgacg taggcctcgc gtttgaaaaa cagcagcata cggcgcaaat
     9781 ctttttccat cgttacgacc cgccagcctt cgggggtttg tttgttgagg aacgcgctga
     9841 aattgacggg gttgaccttt gccgcgccga aaaacaggct ggacaacagg ctttcctgat
     9901 aaatgatgac tttgtattct ttcataggat tccttttaac ataagggatc atcttttcag
     9961 acgacccctt aagttgctaa tgatgcaaaa cagcaaacct tattgcttat tgcccaactg
    10021 cggtaatacc gagccttcgc ccagcgacag caaaccgctt tgcgagtaga tgcccagttt
    10081 ggcgcgggtg tcggtgatgt cgaggttgcg catcgtgagc tgtccgatgc ggtcgagcgg
    10141 ggtaaacgcc gcaccttcga ctttttccat actcaggcgt tcgggttggt aggtcaggtt
    10201 gggcgattcg gtgttcagaa tcgagtagtc gttgccgcgc cgcagttcga gggtgacttc
    10261 gccggtaacg gctttggcga cccagcgttg ggcggtttcg cgcaacatca aggcttggct
    10321 gtcgaaccag cggccttggt agagcaaacg gccgaggcgc aggccgttga tgcggtattg
    10381 ttcgatggtg tcttcgttgt ggatgccggt caccaagcgt tcgtaggcga tgtggaacaa
    10441 cgccataccc ggggcttcgt agatgccgcg cgatttggct tcgatgatgc ggttttcgat
    10501 ttggtcgctc atacccaagc cgtggcggcc gccgatgcgg ttggcttcga ggaagagttc
    10561 gacggggtcg gcgtattctt tgccgttcag tgcaaccggc acgccttctt caaagcgcac
    10621 gctgacttct tcggggctga cttcaacgtt ttcgtcccaa aaggcaacgc ccataatggg
    10681 tttgacgatt ttgatgcccg agttcaaaaa ttccaagtct ttggcttcgt gggtcgcgcc
    10741 gagcatattg gaatcggtgg agtaggcttt ttcaaccgac attttgtagt tgaagccgtt
    10801 ggcaatcaga aattcgctca tttcgtgacg gccgccgagt tcgtcgataa attgctggtc
    10861 gagccagggt ttgtagattt tcagcgcggg attggtgagc aaaccgtagc ggtagaaacg
    10921 ctcgatgtcg ttgcctttgt aggtgctgcc gtcgccccag atgtttacgt cgtcttcttt
    10981 cattgcggaa acaagcatcg tgccggttac ggcgcggccc agaggcgtgg tgttgaaata
    11041 ggcgatgccg ccggtggaaa cgtggaacgc gccgcattgg atggcggcga tgccttcgtg
    11101 tgccaactgc gcgcggcagt cgattaagcg ggcgttttcc gcaccgtatt ccatcgcttt
    11161 tttgggaatg gcgttgtagt cgtcttcatc gggctgaccg aggttggcag tgtaggcata
    11221 aggcagtgcg cctttgagtt tcatccacaa cagcgcggcg gaggtatcaa gtccgccgga
    11281 gaaggcgatg ccgactttct gaccgacggg gaggttttgt aaaatagtat ggttttggct
    11341 cataagggta tccttcggac ttggaaaaag gagttcgtcg tgtttccttt tcgattgaaa
    11401 ccccgccact tggacatctg tccttcgggg cggtagaatc agactttatt tgggaggggc
    11461 gtaatccctt ccaaatcagg acggcacata gggcggtgct ttatgtgtcg tcccgtgtgt
    11521 tgaaacatta tcttataaat ataacccatt gtcagccgtt aaggctgtct gaaaagaagc
    11581 tttgcccgtt tcgctgaaaa tcttttcaga cggtctttac gccatcctca acctgactcc
    11641 ctcccctgcg ggggagggtt ggggagaggg tgcagacagc agcccttaag caaacgctgc
    11701 ctgaaaaacg caagctcgac cctctcccta accctctccc atgggagagg gaacaaatca
    11761 cagcaccatc gacattttca gacggcatcg gtaaagaaac ccatctatga acgcgttcaa
    11821 cctcaccatc atcggcgacg aaatcttaca cggcagccgc caagacaagc atttcgcctt
    11881 tttcaaatcc ctgctcgaat ccaaagggct gaagctcaat caggtgcaat atctgcccga
    11941 cgaacccgat ttgctggtca ggcaactgcg ccgcagcttt tcagacggca taccgacgtt
    12001 tgttaccggc ggcatcggcg ccacgcccga cgaccacacg cgccaagccg cagcggctgc
    12061 tttggatttg cctgtcgtcc gccatcctga agccgccaag tttatcgaag gcgtaacccg
    12121 gaaacgcggt gagccgctcg attcgcccga acacgcccaa cgcctgaaga tggcggattt
    12181 tccccaaggc gcggaattgg tgcctaatcc gtttaataat atcgccggat tttccattcg
    12241 cgagcattat ttcttccctg gttttcctgt gatggcgcac ccgatggcgg aatgggtttt
    12301 ggatacttat tacgtcggcc gcttcaacca aaccaaacgc ggcagccgca gcgtgtatgt
    12361 gtttgagcag cccgaatcgc gcattacgcc gcttatggag catatcgagc aaacttatcc
    12421 cggtgtgcgt tcgtacagcc tgcccagcgt cggttggacg cattcagacg gcacgcaggt
    12481 caaaccgcac attgagttcg gcatcaaagc cgaaggagaa gccgtcaacc ttttggacgc
    12541 ggcgtgggcg gaagtgttgc acagcttgga cggattggga gcagagctga aaaatcgggt
    12601 aaactgaaaa ccgtttgccc aaatcggacg atatttaagg aaaaccatgc aataccgaat
    12661 cagaagagaa aacgaagcac ccgaagcaaa aaatacgggg gaaaccttat gggagcgcga
    12721 catcatgcgc gaagtgctgc tgtccgccta tcaggacagg cgcagggagc ggatgtggaa
    12781 aaacatctgg cgtgccgtca gcaccctgat tctggttgcc ctgattgccg gtattttccg
    12841 aaaagacgaa gccgcattgc agttggcggg caacacgccg cataccgcag tcgtcaacct
    12901 gtacggcgaa atcggcaacg gtgtagaaga ccaggtcaaa aaactcaaag acggtatgga
    12961 ggccgcctac aaaaacccgc aggcaaaagc catcgtcatc cgcgccaaca gccccggcgg
    13021 ttcgcccgtc gtctccaaca ccgcttttga agaaatacgc cgtctgaagg cgcagcaccc
    13081 cggcattccc gtttatctcg tggcggaaga tatgtgcgcg tccggctgct actacatcgc
    13141 ggcggcggcg gacaaaatct atgccgaccc gtccagcatc gtcggcagca tcggcgttat
    13201 cggcagcagt ttcgacgcga ccggcctgat ggaaaaaatc ggcgtgaaac gcagggttaa
    13261 aattgcaggc agcaacaaag gcatgggcga tccgttttcg cccgaaacgc ccgaacagtc
    13321 gaaaatctgg gaagaaatgc tgaccggcat acacggcgaa ttcatcaaag ccgtcaaaac
    13381 cggacggggc ggccgcctca aattccgaca atatcccgac gtgttcagcg gtcgcgtgta
    13441 cacaggcgcg gatgcgctga aagtcgggct ggtggacgga ctcggcaata tttacagcgt
    13501 ggcgcgagac gtggtcaaag cccccgatgt ggtggattac actccgaagg acgatttcgg
    13561 cagaatcttg ggcagacgct tcggcgcaga actcaaagca agcgtgcgcg aagccctgca
    13621 ggccgtccgg tagcgttgtt tacagtattt ctaagggaat accaaaataa aaatatacac
    13681 aaaaacactc acctccccag tatgctgcca ctaagtcagc cgcgtcacta tttaccgtgc
    13741 acttaaagcc gtaaaaggca ggttgtcccc gggaggtttt gtgcatcctg ataccgatcg
    13801 gttgttgttg ctcaaaggac agaaggccgc tgataaacga gattacctgt ttgtcgctat
    13861 tgacgatttt tatactctgc cattttgccg gacaaaaccg cagacagtgc tgccaagttt
    13921 ctgaccgaac atctggccga cccctgcttg tacctgattg agtacgttta ctctgacaat
    13981 gataggtaat ataaagagcc gtccaacatg ctttcggtgc agcttgttat gataatggga
    14041 ttggttggag gcttgcccga tttgcttgtc cgcagaccaa cggtaaggcg gagcgggtta
    14101 tccgtacctt gatggagatg tggcatgagg aacagtcgtt tgacagtccg gaacatcggc
    14161 aaaaggagtt gtgctgcttt attaatttag gggctgccct agatcactag ggaaattcaa
    14221 attaagttag aattatccct atgagagaaa gtcgtctaag ccggtataaa caaaataaac
    14281 tcattttgtt atcgtggcta tgatgtattg gatgcgggcg aatttagcca tttccgtatc
    14341 aatcacagca cacattttgc cgaacgacaa aaccatatta atggaa
//
LOCUS       DAXIEB010000048        14265 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000048 DAXIEB010000000
VERSION     DAXIEB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.048"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..291
                     /locus_tag="ACFOA3_001139"
     CDS             <1..291
                     /locus_tag="ACFOA3_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898151.1"
                     /translation="NQEVFGAKTAENLCLGFGCREGKEFCKGLRLGGSRIAWNRHGRR
                     CADAFGREGGAIGSLEKGNGICGQKKLRVKVGRTVRLSVLLSEQETGQLCPD"
     gene            complement(330..1136)
                     /locus_tag="ACFOA3_001140"
     CDS             complement(330..1136)
                     /locus_tag="ACFOA3_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6898152.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(1137..1883)
                     /gene="pssA"
                     /locus_tag="ACFOA3_001141"
     CDS             complement(1137..1883)
                     /gene="pssA"
                     /locus_tag="ACFOA3_001141"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6898153.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADIVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            2466..2948
                     /locus_tag="ACFOA3_001142"
     CDS             2466..2948
                     /locus_tag="ACFOA3_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6898154.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(3000..4211)
                     /locus_tag="ACFOA3_001143"
     CDS             complement(3000..4211)
                     /locus_tag="ACFOA3_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6898155.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            4434..6872
                     /locus_tag="ACFOA3_001144"
     CDS             4434..6872
                     /locus_tag="ACFOA3_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6898156.1"
                     /translation="MTEKSHKKTVKGRAGSPSPMSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMSDPSWSHSSPVAGDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKRTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGRSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHVGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            7080..8390
                     /gene="tig"
                     /locus_tag="ACFOA3_001145"
     CDS             7080..8390
                     /gene="tig"
                     /locus_tag="ACFOA3_001145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6898157.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            8486..9100
                     /gene="clpP"
                     /locus_tag="ACFOA3_001146"
     CDS             8486..9100
                     /gene="clpP"
                     /locus_tag="ACFOA3_001146"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6898158.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(9153..10418)
                     /gene="ispG"
                     /locus_tag="ACFOA3_001147"
                     /gene_synonym="gcpE"
     CDS             complement(9153..10418)
                     /gene="ispG"
                     /locus_tag="ACFOA3_001147"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6898159.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(10434..11195)
                     /gene="pilW"
                     /locus_tag="ACFOA3_001148"
     CDS             complement(10434..11195)
                     /gene="pilW"
                     /locus_tag="ACFOA3_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6898160.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(11198..12292)
                     /gene="rlmN"
                     /locus_tag="ACFOA3_001149"
     CDS             complement(11198..12292)
                     /gene="rlmN"
                     /locus_tag="ACFOA3_001149"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6898161.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTSAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(12435..12860)
                     /gene="ndk"
                     /locus_tag="ACFOA3_001150"
     CDS             complement(12435..12860)
                     /gene="ndk"
                     /locus_tag="ACFOA3_001150"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6898162.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(12919..14070)
                     /gene="zapE"
                     /locus_tag="ACFOA3_001151"
     CDS             complement(12919..14070)
                     /gene="zapE"
                     /locus_tag="ACFOA3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6898163.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         3479 a         3498 c         3929 g         3359 t
ORIGIN      
        1 aatcaggagg tatttggggc gaaaacagcc gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctccgt ttgggaggca gccgcatcgc gtggaatcgg
      121 cacggcaggc gatgcgccga tgccttcggg cgtgaaggcg gggcaatcgg cagtttggaa
      181 aaaggcaatg gaatctgtgg gcagaagaag ttaagggtaa aagtcgggcg cacggtcagg
      241 ttgtctgtcc ttttgtcgga acaggagact gggcagcttt gtcctgatta attgcttgtg
      301 caaacggtaa aaaggttttt tcgtgtgttt taaagcaggt tgtacagcat ttttccggca
      361 atcaaaagca gcataatgcc gaaggatttt ttgagtttgg cagaagaaag tttgtgggcg
      421 gttttgacac cgagcggggc aaaggcaatg gttgccgcgc tgaggacggc gacggcgggc
      481 aggtaaagga agcccagtga cccttcgggc aatcctgcaa tattcaggcc gttgagcaga
      541 tacgatattg cgccggagag tgcaatcggc caggcaaggc cggatgatgt gccgatggct
      601 ttatgggcgg ggaagccgca gtggattaag aaggggacgg aaagtgaacc gccgcctatg
      661 ccgacccagc tcgacattgt gccgaacagt gtggaaaccg cagtcagtcc gggcagtccg
      721 ggcagcgggc gggatgccgt ctgagggtcg gtatgcagtg ttttgaatgc gacggcggtt
      781 aaaaacagga tgaagaaaat ttgaagcccg aacgcgggga tatattttgc ggagagtgcg
      841 cccgtgaata cgccgaatat catacccggc atcatcgtaa atacggtttt ccagtcgacc
      901 gcctgttttt tgtgctgccc cagcatactg gaaaaggcgg tgaagaccat gacggcgaag
      961 gatgtgccga cggcgaggtg ttgcgcgtaa ggatgttgtg ccaaaccctg caaatcaagc
     1021 acccataaaa cgacagggac aatcagcgtg ccgccgccta cgccgaacag gccggcaata
     1081 aaacctgccg cactgcctac ggcaagcagg attaagataa tgtcccaatg ccacatttaa
     1141 tccgcctttc tgtatttttt ccaaaatcgg cgcgccgcca taatgtagcc ggacaggctg
     1201 tatccgagaa agaacaggaa gaggacgagc gacggttccc aagtgaccag aagcagcagt
     1261 aagacggcaa gcagcattcc gacaaagggg acttgtctgc ggatgttgat ttctttaaaa
     1321 ctccaaaaag ggatttggac aatcatcgac aggccggcaa acagtgtgat gcccaatgcc
     1381 caccagtgga cggcggggaa tttttcgacg ctgtggttga cccaaatcag cccgacaatc
     1441 agcgcggcgg cagtcggact gggcacgccg ataaaccagc gtttgtccac cttgccgatg
     1501 agcgtgttga acagggcgag gcgcagggcg gcgcaggcgc agtagatgaa ggcgacggaa
     1561 taaccgattt tgccgaactg ccaaagctgc catttgtagg caataagcgc gggggcaacg
     1621 ccgaagctga ctatatcggc aaggctgtcg agctgttccc cgaacgcgct ttggctgttg
     1681 gtcagccgcg ccacgcgccc gtccataccg tccagcagca tagagatgaa taccgcgatg
     1741 gcggcggttt cgtaacgtcc gtgcatggat tgggtgattg cgtaaaacgc ggaaaacagc
     1801 gcggcgatgg taaaggagtt gggcagcagg tagatactgt tttgacgcag ggagcgttgc
     1861 ggggtgttgg tcggggtttc catagctgaa gtgtctcacg gacgtggaga atcgataggg
     1921 gattataacc caaaccatta tcgaacaata cgccctttag gatgaagccc tcggaggcgg
     1981 tttgtttttc tgatggcgga tgctttgcgg ggcggtttga ctctgcctgc caccgggtgt
     2041 gttgtttcag atttttgccg gatggatatt gttgccgggc atttctgggc ttgccggaag
     2101 gtatcggggg gagggcatcg gaatatgcgg cgcgccgttc aaaccggggc ggcgtttccc
     2161 tattttcagg gggttgcagg tttcaacaaa ttgaaagata ttttttgcat ttccgaccgt
     2221 atgctgattt tgaaatggga taaattatag gacaaggttt ttcgcccgcc gcaaaggttg
     2281 gccgcaatgc cgtctgaaaa caacggcttg ccgctaaggc ggcggtatgc ggatttgcgc
     2341 tgccgaaatc ggtttgccat acccggcggt gcaatctgcg gggagggata agccgataag
     2401 gcgggactta tcctgttttt tgtttataat gcaacagaat ttggacgaac cgtcgggaat
     2461 ttatcatgat gaaaaagata ctggccgtat cggcactatg cctgatgact gcggcggcac
     2521 gggctgccga tacttacggc tatctcgccg tttggcagaa tccgcagaat gcggacgatg
     2581 ttttgcaggt taaaaccaca aaagaagatt cgacgaaaag cgaagcgttt gccgagttgg
     2641 aagccttttg caaaggtcag gacacgcttg cgggcattgc cgaagacgag ccgactggat
     2701 gccggtcggt cgtgtcgctg aacaatacct gtgtcgcgct ggcatacccg aaagccttgg
     2761 gcgtgatgcg cgttgaaaac gccgtcgtga ttacttctcc gcgttttacg agcgttcatc
     2821 aggtcgcact caaccagtgc atcaaaaaat acggcgtaca gggacaatgc ggcttggaaa
     2881 cagtgtattg cacatcttct tcttattacg gcggaactgt gcgctctttg attcaaaatc
     2941 tcaaataaaa cggaaaatgc cgtctgaaag atgttcagac ggcatttcta tatcgacggt
     3001 caggattctt tcggatcggg cagcaggctg ttcaacataa tggcaagtac ggcgcacaag
     3061 cccacgccgg caaagctgaa gctgcccaat ttgagcgtca tgccgccgat gcccgtggtc
     3121 agtaccgagc tgacgatgac caggtttttc ggcagcatca aatcgacttt ggcatcaatc
     3181 agcgttttca cgcccaaaga agcaatcgtg ccgaacagca gcagcataat gccgcccatt
     3241 actggcatcg gaatggaagc caaaaacgca ttgaatttgc cgaaaaacgc catgcagacg
     3301 gcaaaaaccg ccgcccaagt catgatgacg gggttactgt ttttggtaat catcaccgca
     3361 cccgttactt cgccgtaggt cgtaaccggc gggccgccga tcagacccgc aacgcacacg
     3421 cccaaaccgt cgcctgcaag ggttttgtcc aagcccgggt ctttcgtata gtctttcccc
     3481 gtcacattgc cgattgccat gatgccgccg atgtgttcga tggcgggggc gacggcaacg
     3541 ggcagcataa acagtgcagc ctgccagttg atctgaggcg tttcaaaatg gggaacggcg
     3601 aaccagggcg cgtgtgcaat gcttgccgtg tccaccagtc ccatcagcag tgccaaaaca
     3661 taacccgaag cgacaccgat caagatggga atcagcttca tcatcctgct gccgaaaacc
     3721 gatacgatgg cggtaacggc aaaggtaaag ccggaaagaa tcagcgaatc ggtatagtcg
     3781 atgacctgtt tgccgtccgc ctgacccatt gccatgctgc ttgccgccac ggcaacagac
     3841 agaccgatga ccatgatgac ggggccgatg acgaccggag gcagcagttt gtgtaccgct
     3901 gccagtccgc gccaacggat cagcgcggca aacacaaaat acataaagcc ggcggcaaac
     3961 agtccgaaca tggtggaagg cagcccccat tcgccgacgg agtagataat cggtgcgata
     4021 aaggcaaacg aagaaccaag aaaaatcggc actttgcgtt tggttgtgat ttggaacagc
     4081 agcgttccca agcctgcgcc caaaagcgca agagccggat tcagaccggt cagcagggga
     4141 accagcacca ttgcgccgaa tgccacaaat aaaatctgtg caccggaaac ggcaagtttc
     4201 agttggttca taccatcttc tttcagtcgg ttcaaacggc acggattata ctggtttgcc
     4261 gagggtctgc gtaaaaatac atacgtcggg caacagatat gccgtctgaa aggcaacgtt
     4321 tgtctgttgc aggatgaaag caggagaaat ggagtataat ccgacacttt ccatattcaa
     4381 tccgcaagcc gtccggcctc cttatttcag acggcatttt ccggtaatac ggcatgacag
     4441 aaaaatccca taaaaaaaca gtaaaaggca gggcgggaag cccgtcccca atgtccgcac
     4501 gcaacaagaa agccgacaac ggcgcacggg gcaataaagt ttccgagcgg ctcaaggcgg
     4561 tcaaagagtt gcagaaaacc gaagccaaaa aggcgcgtcc cgaacatgtc gtcaacctta
     4621 ttggcgacgc gctgtggttg atgggtttgg cggcaaccct gtatttggcg atttccctga
     4681 tcagtttcga tatgagcgat ccgtcttggt cgcacagttc gccggttgcc ggagatgccg
     4741 ccaattgggg cggactgttc ggcgcgtatg ttgccgatgt cggctattat cttttcggct
     4801 ggtcgttctg gtggtggata gcggctgcct gcgtcatgct gtataaaaat ttccgtctgc
     4861 acgcaaaacg gacggaaaac gaggcataca accacaaaat cgctgccgcc gcgctgtttg
     4921 tcctgacggt cttcagcccc gtcttggagt attttgtgct gggcggaaaa tatgccgact
     4981 ccctgcctgt cggagcaggc ggtatggtcg gcatacgcgt cggcgcagtg tttgcgtggc
     5041 tgttggggag atcgggcagc ctgctgatta tcttggttgt tctgctgttg tcgttgtccc
     5101 tgctggtgca gatttcatgg ctggaatttt tgaacggtgc gggcagggcg gttcaaaacc
     5161 gcctgagtgc cttatccggc aaggtcatgg ctttaggaaa acgccggccg aatagcaaaa
     5221 cagacggtgt cgatacccaa aatacacggc gcatggtaaa agaagccaag aatattacgg
     5281 ccaaacccgt tgccttgccc gaaggcagca gcagcaaccg caaatccgtc gcggtttccg
     5341 tcgcgccgtc gcccaaaatt caggtttctc tgtttgaaga tgacgaacct cggcaggcgg
     5401 gcgaatacca caagcctaca ttgaacctat tgcggattcc tgacagcgaa cccgtcagca
     5461 tcaatcccgc cgaattggag cgcactgccg aactgatcga atccaaactg gcagaattcg
     5521 gcatcggcgt acaagtcgta tccgccacat ccggccccgt catcacgcgc tacgaaatcg
     5581 aacccgcgca aggtgttaaa ggcagccaaa ttgttgcctt gtcgaaagat ttggcacgct
     5641 ctatgtcgct gcaatccgtg cgtatcgtcg aaaccatcgc aggtaaaaac acgatgggca
     5701 tcgagttgcc caacgacaaa cgccaagacg tgatgttgag cgaaatcttg tcctcgcccg
     5761 tgtttgccgg agccaaatcc aagctgaccg tcgcgctggg caaagacatt gccggtaccc
     5821 ccgttgtcgg cgacttggcg aaaatgccgc accttttggt cgccggtatg actggttcgg
     5881 gcaagtccgt cggcgtgaac ggcatgatta tgtctatgct tttcaaagct acgcccgacg
     5941 aagtccgctt cattatgata gacccgaaaa tgctcgagtt gagcatttac gacggcattc
     6001 cgcacctgct ctgtcccgtc gtgaccgata tgcgcgaagc agggcaggcg ttgaactggt
     6061 gcgtcgccga aatggaaaaa cgctaccgcc tgctttccca cgtcggtgta cgcaatctgg
     6121 aaggcttcaa ccaaaaagtc gaagccgcaa aagcggcagg caagccgctg ctcaatccgt
     6181 tcagcctgaa ccccgacgag cccgagccgc tggaaaaact gccgttgatt gtggtcgtta
     6241 tcgacgagtt ggcagacctg atgatgaccg aacgcaaagc cgtcgaacag caaatcgccc
     6301 gtctcgccca aaaagcgcgc gccgccggta tccatatgat tgtcgcgacc caacgtccca
     6361 gcgtcgatgt cgttaccggc ctgattaaag ccaacatccc gacgcgtatg gcgtttaccg
     6421 tgcaaagcaa aatcgacagc cgtaccatcc tcgaccaaat gggcgcggac gaactgctca
     6481 aatatggcga ttcgctgttc ctccagcccg gcagtgccga accgacccgc ctgcaaggcg
     6541 cgtttgtttc agacgacgaa gtacatcaag tcgtcaacta cgtcaaatcg caagccccag
     6601 ccgactatat tgaaggtctg ctcagcggcg aggccgcgct ggaaactgcc aatatcgtta
     6661 atccgaatgc agacagcgac gaattgttcg atcaggcagt cgcctatgtt ttggaaagca
     6721 aaaaaacctc catttcgtct ttgcagcggc agttgcgtat cggctataac cgcgcggcaa
     6781 acctgatgga ggcactggaa aatgcgggtg tcgtttctcc caccgacctc aacggcagcc
     6841 gtaaaatttt ggcgcacaag gaccatttgt agcccgtatt gcaaaatgcc gtctgaacgg
     6901 cggaattggc gtttcagacg gcatattatg tttcaggcga aacattgtga tatacttgcc
     6961 agctaaaatt tcccctttgc ggcaatgcgg ttcaaatatc gtaccgttgc gctgtttgct
     7021 tccccatgta gggaagaaag tttatcattt tatcaacaca acaaatttaa gggcttatga
     7081 tgagcgtaac tgttgaaact ttagaaaatc tggaacgcaa agtagtgttg tccctgcctt
     7141 ggtccgaaat caacgcagaa accgataaaa aactgaaaca aacccaacgc cgtgcaaaaa
     7201 tcgacggttt ccgtccgggt aaagcacctt taaaaatgat tgcccaaatg tacggtgcga
     7261 gcgcacaaaa cgacgtgatc aacgagctgg tgcaacgccg cttctacgat gttgccgttg
     7321 cccaagagtt gaaagtggca ggcttccccc gttttgaagg cgttgaagaa caagacgata
     7381 aagagtcttt caaagttgcc gccatttttg aagtgttccc cgaagtcgtt atcggcgatt
     7441 tgtctgcaca agaagttgaa aaagtaaccg cttccgtcgg tgatgccgaa gtggaccaaa
     7501 ccgtagaaat cctgcgcaaa caacgcaccc gcttcaacca tgtcgaacgc gaagcccgaa
     7561 acggcgaccg cgtcatcatt gactttgaag gcaaaatcga cggcgaacct tttgccggcg
     7621 gcgcatccaa aaactacgcc ttcgtattgg gcgcaagtca aatgctgcct gaatttgaag
     7681 ccggcgtagt cggcatgaag gctggcgaaa gtaaagacgt taccgtcaat ttccctgaag
     7741 actaccacgg taaagacgtt gccggtaaaa ctgccgtgtt caccattacg ctgaacaacg
     7801 tttccgaagc gactctgcct gaagtcgatg cagattttgc aaaagccttg ggtattgcgg
     7861 atggcgacgt tgccaaaatg cgcgaagaag tgcagaaaaa cgtaagccgc gaagtggaac
     7921 gccgcgtaaa cgaacaaacc aaagaatccg taatgaacgc gctgctcaaa gccgtagagc
     7981 tgaaagcacc tgttgctttg gtcaatgaag aagccgcacg cttggcaaac gaaatgaaac
     8041 aaaattttgt taaccaaggt atggctgatg ctgccaactt ggatctgcct ttggatatgt
     8101 tcaaagaaca agccgaacgc cgcgtatctt taggtctgat tttagccaaa ctggttgacg
     8161 aaaacaaact ggaaccgact gaagagcaaa tcaaagccgt tgttgccaac tttgcagaaa
     8221 gctacgaaga tcctcaagaa gtgattgact ggtactacgc agatccttcc cgcctgcaag
     8281 ccccgacttc tttggcggta gaaagcaacg tcgttgattt cgttttgggt aaagccaaag
     8341 taaacgaaaa agctttgtct tttgacgaag tgatgggcgc gcaagcctga ttatcctgaa
     8401 aatgccgtct tagggcagtt tttgaaagca ccgaagccgc ttttaaagcc cgctttggtg
     8461 cttttacatc atgaaaggag acgaaatgtc ttttgataac tatcttgtcc ctaccgttat
     8521 cgagcagagc ggtcgcggtg agcgtgcatt cgatatctat tctcggcttt tgaaagagcg
     8581 catcgtattc ttggtcggcc cggtaaccga cgagtccgcc aatctggtgg ttgcccaact
     8641 gttgtttttg gaaagtgaga atccggataa ggatattttc ttctatatta actcgccagg
     8701 cggttcggta acggccggta tgtcgattta cgacaccatg aattttatca agcccgatgt
     8761 atcgactttg tgcttggggc aggcagcaag tatgggcgcg ttcttattgt cggcaggcga
     8821 aaaaggcaaa cgtttcgccc tgcccaacag ccggattatg attcaccagc ctttaatcag
     8881 cggcggcttg ggcggtcagg catccgatat tgaaattcac gcacgcgaac tgttgaaaat
     8941 caaagaaaaa ctcaaccgcc tgatggcgaa acattgcggc cgcgatttgg cagatttgga
     9001 gcgcgacacc gaccgtgata atttcatgtc tgccgaagaa gcaaaagaat atggtttgat
     9061 cgaccaagtt ttggaaaacc gcgcttcttt gcagctttaa accttgtgaa gtagacaaat
     9121 gccgtctgaa aacagttcag acggcatttg tcttataggg actgtattgg gatgattttg
     9181 ttctggttgc gtttggagcc gttttcgccg tagttggttt tgacatactc ttcaacaata
     9241 gccaaaaatt ccgttgcaat gttgtcgcct ttcagcgtta ctttgcgttc accatctaca
     9301 taaacaggcg cgacgggtgt ttcccccgta ccgggcaggc tgatgccgat gtcggccaat
     9361 ttgctttctc cgggcccatt gacaacgcag cccattacgg caacgttcag ggattcaacc
     9421 ccgggataaa gggtacgcca tatagacatt ttttggcgca ggtaattttg aacatcttgt
     9481 gccagctctt gaaatacggt actggtggta cgcccgcagc cggggcaggc ggtaaccatc
     9541 ggcgtaaacg aacgcaatcc catagtctgt aaaatctctt gcccgacgac gacctcctga
     9601 gtacgcgggc tgccaggttc cggagtcagt gaaatgcgga tggtgtcgcc gattccttct
     9661 tgaagcaaga cggataatgc cgccgttgat gcgacaatgc ctttgctgcc cataccggct
     9721 tcggtcaaac ccaaatgcag cggataggcg caacggctgc ccagttcgcg gtaaacctga
     9781 atcaaatcct gaaccgcgct gactttgcac gacaggatga ttttgtcttc gggcagtccc
     9841 aatagaacgg ctttttcggc agattccaaa gcggagacaa tcagtgcttc cttcgtcact
     9901 tcttcgggcg gtttcggcgc ggaagaagcg aggttggcat ccatcatacg tttggcgagg
     9961 ctttgatcca aagaacccca gtttacgccg atgcggacgg ctttatcgtt ttcagcagcg
    10021 gtacgaatca taaaggcaaa tttttcatcg ccttttacgc ctttgccgac attgccggga
    10081 ttgatgcggt atttggacaa tgctttgccg cattcgggaa attccgccaa caggcgttcg
    10141 ccgttgaagt ggaaatcgcc gataagtggt gtggcatagc ccatatcgtc caagcggcgg
    10201 cggatttcgg caactttgga cgcggcttcg gggctgttga cggtaatacg caccatttcg
    10261 gatccggcat cgctcaattc cttaatctgc aatgcggtgg cttttgcatc ggcagtgtcg
    10321 gtgttggtca tagattggat aacgacgggt gcttctgaac cgacggtaat atgatcgatg
    10381 cggacttgat gcgtcttgcg gcgttggagt gtgttcatat ggtttgaatc tgtttattga
    10441 ccgatgagga cggtttgcaa ttcttccgag taggggaaat ttgcctgcaa ttgtgcttca
    10501 tattcgtatg ccgcctgtgc gttgccgagg gctttggcaa ttttccagcc tagcagcaaa
    10561 tcatcggcct gaaggacttc taccctgctt tggtattttt taaagtagta atcggcatcg
    10621 cccaactgcc cggccagcat tttggtgcgc gccagttctt taaatgcggg tgggaactgc
    10681 ggctgggcgg cgagggaacg tttcaaatag gcttccgcca atccgaattg cccctgtttt
    10741 gcgctgcata tgcctttatt caggttggca atataagggg tcgggtaggt ggggtcggcc
    10801 agggctttgt cgaaatatgc catagattcg gcagggcggt tgagcctgcc gcacaggaac
    10861 cagccgtagt tgttgttgat ttcggcactg tcgggtttga tggagagggc ttgccggaaa
    10921 ctttcctgcg ccttgtcgtt aactttcagg tattggtaga tttcggcacg gaccagccag
    10981 gcaagctcgt ttttagggtc cgatttcagg gcgtcttcaa tacttgccgt cgcctgacgg
    11041 tagtcctgac cgcgcatata ttccattgcc aactgggttt tgatattgga aacctgattg
    11101 gctttttctg cccgcgaggg gcggtaggaa gtgctgcacg cgcccaaggc aaggacaagt
    11161 aataaagaga ttcgtttgga tggcttaaaa ggcataatta cccctgttgt ccgattaaaa
    11221 tctgctgcca tttttgttgg cggcgcgttt tatcctgaac ctgccccgcc aactgtccgc
    11281 aggcggcatc gatgtcgtcg ccgcgcgttt ttcgtacggt aacgacaaat cctgcctgct
    11341 gcaaaatatc gcggaacaca cggatgttct cattgctgga gcgttcgtat ccggagtttg
    11401 ggaagggatt gaacggaatc agattgaact tgcagggaac atctgtgacc agttcgatca
    11461 gttcgcgcgc atgttgcgcc ttatcgttta ttccgtccaa catgacgtat tcgaaagtga
    11521 tgaaatccct gggtgctttg accagatagc gttggcatgc ggccatcaat tctttcaagg
    11581 gatatttttt gttcaacggt acgatttggt tgcggacttc gtcattggaa gcgtggaggg
    11641 aaaccgccaa agccaccggc atgacatcgc gcaacctgtc catttgggga accatacccg
    11701 aagtggaaac ggttacgcgg cggcggctca aaccgtagcc gtggtcgtcc agcatgatgc
    11761 ttaaggcggt aacgacattg tcgaagttcg ccatcggctc gcccatgccc atcatgacga
    11821 cgttggaaat aacgcgctcg tttttcggtg taacgcccat ggctttgttt gcccaccaca
    11881 attgcccgat gatttcggca gaggtcaggt tgcggttgaa gccctgccga ccggtcgaac
    11941 aaaatgtaca ttccaaagcg cagccgactt gagaggaaat gcagagcgtg ccgcgatccg
    12001 attcggggat gaagacggtt tccacgccgt tgcccgtacc gacatccaaa agccattttc
    12061 gagtgccgtc tgaagatttt tgagacatca tcagcttggg aatttcgatg cctgcctgtt
    12121 cgttcagttt atggcgcaac gattttgcca aatcggtcat ttcgtcaaaa ttttgcgcgc
    12181 cggattggtg catccaacgc ataacctgtt tggcacggaa aggtttttcg cccatatcgg
    12241 caaaatgtcg ggtcagccct tgaaggtcgt agttgagcag attggttttc atgtgtttgt
    12301 gtttttctta aatcaaggct tcaaataaaa aattgcaggg caggcaagtt tgatttgaag
    12361 ccttttgcaa cagagttttc agacggcatg gatatattta tgccgtctga agaggagttg
    12421 ggcggtgtat tgtatcaacg ggggcagatt tcggtttggc tgaaaaagta ggcaatttcc
    12481 aaagcggcat tttccacgct gtcggaaccg tgtacggcat taatgctgac cgaagtggca
    12541 aagtccgcgc gtatcgtgcc ttcggcggct tcggaaggat tagttgcacc catcagttcg
    12601 cggtttttca ggacggcgtt ttcaccctct aatacctgaa tcataaccgg accgccggtc
    12661 ataaattcaa ccaatccggc gtagaagggg cggtctttat gaaccgcata aaattcttgc
    12721 gcctctttga gagtaagctg cttcattttg gcggcaacga ttttcagacc gttctcctca
    12781 aagcggctgt atattttgcc gataacattt ttgccgacgg catcgggttt gatgatggag
    12841 atggtacgtt caatcgccat gctatatcct tatttttact taagaagaat caaatcggta
    12901 ttctatcaaa aaaataactt atccgcctga agccttggga gatagggtca ggtgcggctg
    12961 ttccaaataa acttcggact gcatttcgac catccggctg gcggtgcggg taaattcttc
    13021 ggcaaaatcg ccttccgtat agatatggtt gacatctacc gcgccggtgg cacacagttt
    13081 gacccggaaa tcgtagagta cgtcaatcag ccaagtcagc cgccgcgcct ccgccttttc
    13141 ttgcggtgag agttgttcca aacctgaaat aaaaaccatt tcataatgtt cggccaaata
    13201 cagatagtcg gactgtgagc gggggccgaa gcacagtgcg cggaaatcaa accatatggc
    13261 acggccggac tcggctttgt ggggaatctc ccgaccgtgg atggtgctga tgccggggtt
    13321 caaatcggta atgcctgtca tttctttgaa cagttttgcc agttttgcct cattttcttc
    13381 attggcaggc gtaaagaaaa tctcggcggg gcggagggta cgcagtcggt agtcttcacc
    13441 gccgtcaacg tttaagacgg tcaggctgga ctcgatgagc gcgattgtgg gaagaaaact
    13501 gctccggttt tgaccttgcg ggtagagttc ggaaggcgcg tagtttgaag tcgccaccaa
    13561 aacaacgccc tcgttaagca ggttttccag cagacggcct aaaatcattg catccgcaat
    13621 atcgctgaca tgaaattcgt caaaacataa tacgcgggtt tctttggcaa tctcggcggc
    13681 aacggatttc aacggattgc tttcgctttt cagggttttc agcctctggt ggatttctgc
    13741 cataaaggca tgaaagtgga cgcggcgttt gcggcggtaa gggaggcagc cgaaaaaagc
    13801 gtccatcaga aagcttttgc cgcgtccgac cccgccatag aaataaagcc ctttggggac
    13861 ttgcggggaa cgcaaactcc tgcctaaaaa acggtttctt ttgcgtttga acatcatcaa
    13921 ttcggtccaa agccgatcga ggtgttcgat ggcagctgcc tgcgcgtcgt cgcggatgaa
    13981 gttgggcagt tgtgaggcag cctgatacca agtcagcggg ctgtgatttt caaacggcgg
    14041 ggctttaaaa agttggtctc tattactcat atttaacctt gttatacttc ttgcggctaa
    14101 gaaagaaaat gcttaccgtc ggtattataa atgatttcct tcaaacttgg cattttccgt
    14161 ggaaaagaat aatgacgcgc acaagggggt ataagcaaag gcggcgtttc aatatggtgc
    14221 agatatgagc tcaaatgcct tttatagtag attaaattta aacca
//
LOCUS       DAXIEB010000049        14052 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000049 DAXIEB010000000
VERSION     DAXIEB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.049"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(111..938)
                     /gene="fghA"
                     /locus_tag="ACFOA3_001152"
     CDS             complement(111..938)
                     /gene="fghA"
                     /locus_tag="ACFOA3_001152"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6898164.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNLENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(947..2056)
                     /locus_tag="ACFOA3_001153"
     CDS             complement(947..2056)
                     /locus_tag="ACFOA3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6898165.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            2204..2611
                     /locus_tag="ACFOA3_001154"
     CDS             2204..2611
                     /locus_tag="ACFOA3_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6898166.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2962..3276)
                     /locus_tag="ACFOA3_001155"
     CDS             complement(2962..3276)
                     /locus_tag="ACFOA3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6898167.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3287..4972)
                     /gene="rpsA"
                     /locus_tag="ACFOA3_001156"
     CDS             complement(3287..4972)
                     /gene="rpsA"
                     /locus_tag="ACFOA3_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6898168.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(5128..5784)
                     /gene="cmk"
                     /locus_tag="ACFOA3_001157"
     CDS             complement(5128..5784)
                     /gene="cmk"
                     /locus_tag="ACFOA3_001157"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6898169.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(6028..7397)
                     /locus_tag="ACFOA3_001158"
                     /pseudo
     CDS             complement(6028..7397)
                     /locus_tag="ACFOA3_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            7666..7772
                     /locus_tag="ACFOA3_001159"
                     /pseudo
     CDS             7666..7772
                     /locus_tag="ACFOA3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7974..8666)
                     /locus_tag="ACFOA3_001160"
     CDS             complement(7974..8666)
                     /locus_tag="ACFOA3_001160"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6898170.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGMLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVLHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(8734..10710)
                     /locus_tag="ACFOA3_001161"
     CDS             complement(8734..10710)
                     /locus_tag="ACFOA3_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6898171.1"
                     /translation="MSKLKTIVLTASGLSVCSGSLYAQNTSSHQIGLAIMRLNSSILD
                     LHPTKQYFQSGSLWGELRQGFQMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAIEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISASPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            10889..11554
                     /locus_tag="ACFOA3_001162"
     CDS             10889..11554
                     /locus_tag="ACFOA3_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6898172.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVSSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            11606..12433
                     /gene="mutM"
                     /locus_tag="ACFOA3_001163"
     CDS             11606..12433
                     /gene="mutM"
                     /locus_tag="ACFOA3_001163"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6898173.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            12521..13288
                     /locus_tag="ACFOA3_001164"
     CDS             12521..13288
                     /locus_tag="ACFOA3_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6898174.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(13599..13910)
                     /locus_tag="ACFOA3_001165"
     CDS             complement(13599..13910)
                     /locus_tag="ACFOA3_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6898175.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         3248 a         3221 c         3861 g         3722 t
ORIGIN      
        1 ccgtagagtc ggctccttcc ggctcagacg gcatacgttt atgccggcac ttcttaccgc
       61 cctcgtgccg gctatcccaa aactgctttg aacactgaac gctctttggt ttacttcaaa
      121 aacgcggcgt gataagcaat atgctcgcca ataaaactgg cgatgaagta atagctgtga
      181 tcgtagcctt tatggaaacg cacatcgacc ggctggtttg ccgcacggca ggtttcgata
      241 aaatcttcgg tacgcaattg tgtcggcaaa aactcatctt ccaagccttg atcgatgcgc
      301 ataccttgca ctttatagcc ttgttgaatg agtgagttag catcatattg ctgccatttt
      361 tcacggtctt tccctaaata agcagtaaag gctttttctc cccacggcac gaggcttggc
      421 gataaaatag gcgaaaaggc agaaacactt tgataacgtt cctgattccg cagcgccaat
      481 accaatgcgc cgtgtccgcc cattgaatgt cccataatgg aacgtttgcc gttggtagga
      541 aagtgtttct caatcagacg gggcagctcg ttcaaaatgt aatcatacat ttgataattc
      601 gccgcccaag gctgttcggt cgcattcaaa taaaagcctg cactctgtcc taaatcgtaa
      661 gcatcatcgt tcggcacttg ctctccgcga gggctggtat cggaggccac cacaattact
      721 tgatgttctg ccgcataacg ctgaaagcct gatttggtaa tgaaattttg ttccgtacac
      781 gtcaagccgg aaagccaata aattacgcca agaggtcgat tttctagatt atttggcaaa
      841 tagacggcaa atttcatttc gcattgaagc gtttgggcat tgtgtgccca aacttgttgc
      901 gaaccgccaa aaatttgatg ttgttcaatc agtttcatcg cataccttag tagtgaataa
      961 cggcgcggat cgatttacct tcgtgcatta actcaaaggc ttcattgatt tgatcgagtg
     1021 tcattgtgtg ggttacaaac ggttctaact caatgtcgcc tttcattgaa tcttccacca
     1081 ttttcggcag ttcagagcga cctttcacac caccaaatgc agagcctttc caaacacgac
     1141 ctgttaccaa ctggaacgga cgcgttgaaa tttcttgtcc tgcacctgct acgccgatga
     1201 taatggattg accccaacca cggtgtgcac tttctaatgc ctgacgcatt acgtttacgt
     1261 tgccgataca ttcaaaggta tggtcaatgc cccatttatt aatgtctaac aacacatctt
     1321 tgatcggttt atcgtaatcg ttcgggttca aacaatccgt tgcaccgaac tgttttgcca
     1381 actcaaattt tgatggattg gtatcaatgg cgataatgcg gccggctttg gcttgacgcg
     1441 caccttgcac caccgccaaa ccaatcgcgc ctaagccaaa cacggcaaca gagtcgcctt
     1501 cttgcacttt tgccgtatta tgtaccgcac caatacctgt ggtaacgccg cagccgagca
     1561 aacatacttg ttcatggttg gcttcagggt tgattttcgc cagtgaaact tcggcaacaa
     1621 cggagtattc actgaaagtc gaacagccca tatagtgata gattggctga ccttgataag
     1681 aaaaacgcgt cgtgccgtcc ggcattaagc ctttaccttg tgtatcacgc actgagacgc
     1741 acaagttggt tttacctgaa caacaaaact cacattcgcc acattcggcg gtgtaaagcg
     1801 gaatcacgtg atcacccggt tttacgcttg acacaccttc gcccacagca acgaccacac
     1861 ccgcaccttc gtgtccaagc accacaggga atacgccttc aggatcgctt cctgataacg
     1921 taaacgcatc agtatggcac acgccagtgt gggtattgcg gattaacacc tcgcctttac
     1981 gcggcatttc tacgtcgatt tccacaattt gtaagggttg gttaggggcg aatgccaccg
     2041 ccgcacgaga tttgatggtt gaatcggctt gtttcatttc cattgtcttt cctcttaatt
     2101 cattgacaaa gttgccttgc ctgagcaaca tagcaacagt ttacacttta gagttaactc
     2161 taaagcaagt gttttttact gtttttgttt agaaggaggg aaaatgactt atactactgc
     2221 caaagccgcc gaaaaaatag gcatctccgc ccacacctta cgtttttacg acaaagaagg
     2281 tttgttgccc aatatcggac gtgatgaata cggtaaccgc tgttttaccg ataacgattt
     2341 gcaatggttg ggcttattgc aatgcttgaa aaatacggga atgagcttaa aagacatcaa
     2401 acgctttgcg gaatgtaccg tcattggcga cgataccatt gaagaacgcc tttccttgtt
     2461 tgaaaatcaa atagaaaatg tgaagtgtca aattgccgaa ttaaaacgct atttagattt
     2521 gcttgaatac aaattggcgt tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt
     2581 aaatttgccg caaattcctg aaacggctta gttttagtgc aataagcagg atttttgtcc
     2641 tgttcgtaat agaaatcaaa aatttgttgt ttaaaaagcg gattgtaagc aatgatatag
     2701 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtgtaaat agtacggaac
     2761 cgattcactt ggtgcttcag caccttagag aattgttctc tttgagctaa ggcgaggcaa
     2821 cgccgtactg gtttttgtta atccactata aaaaaatcca cgctctaatc cgttgcttgc
     2881 ttggctcaag gtattgaagc agcggatgaa gttaaaccac agaaaaaacg ccgcgtaaca
     2941 gcggcgtttt gatgctaagg tttaattggc attttctttt aaagccaagt cgacccgctc
     3001 gcgcaattct ttaccgggct tgaagtgggg tacatgtttt tcaggtactt ccacacgctc
     3061 gccggttttg ggattgcgac cgatgcgggc aggacgatgg ttcaaatcga agctgccgaa
     3121 accgcggatt tcgatgcgtt gacctcgggc aagcgatcta gtcatggtgt caaccaagac
     3181 ttttacgctg tactctacgt cttttgccag aagatgcgtg ccgtttttgg cggcaaacac
     3241 ttctgccaaa cgaaccatta actcagactt tgtcatgtct gcaaccttat tcttgttcgc
     3301 cggagagttt ggctttcagc aggtcgccca agctggtggt gccggcattc gcattggcgg
     3361 cggcattgac ggagttcagt gcttcgcggc tttctttggc atctttggct ttaacggaaa
     3421 gtttgatgct gcggtttttg cggtcaacgg taacgatgac ggcttcaact tcgtcgcctt
     3481 ctttcagttt ggtggtcaaa tcttcaacgc ggtcggctgc aaattcggaa gcaggcaggt
     3541 agccttctac ttcgtcagac agggcgataa cagcaccttt ggcgtcaaca gatttcacgg
     3601 aacctttaac caaagaacct ttgtcgttca cgctgatgaa gttgccgaac ggatcgcctt
     3661 ccagttgttt gatacccaag gagatgcgtt ctttttccac gtcgattgcc aatacgacgg
     3721 cttcgacttc ttcgcctttt ttgtatttgc gtacggcttc ttcgccggat tcggtccagg
     3781 acaggtcgga caggtgaacc aaaccgtcga tgccgccggg caggccgacg aatacgccga
     3841 aatcggtaat ggatttaacc gcgccggaga ttttgtcgcc tttgttgtgg ttggcggcaa
     3901 actcttccca aggattggct tggcattgtt tcatacccaa agagatacgg cggcggcctt
     3961 cgtcgatttc caaaatcatg acttcgactt cgtcgcccag ttgtacgact ttgctcgggt
     4021 gtacgttttt gttggtccag tccatttcgg agacgtgtac caaaccttcg atgccttgtt
     4081 cgatttcgac gaatgcgccg tagtcggtca ggttggatac tttgccgaac aggcgggtgc
     4141 cttgaggata acggcgggtc agaccgctcc aaggatcttc gcccagttgt ttcataccca
     4201 aggaaacgcg ttgtttttct tggtcgaatt tcaatacttt ggcttcaact tcctgaccga
     4261 cttccaagac ttcactcggg tgtttcacgc gccgccatgc caaatcggtg atgtgcaaca
     4321 gaccatcgat gccgcccaag tcaacgaatg caccgtaatc ggtaatgttt ttaacgatgc
     4381 ctttgatgac ggagccttct tgcaggtttt ccagcagggc tttgcgttct tcacccaagg
     4441 tagcttccag aacggcgcgg cgggacacaa caacgttgtt gcgttttttg tccagtttga
     4501 tcactttgaa ttcaatttct ttaccttcga agtgagaagt gtcttttaca ggacgtacgt
     4561 cgaccaaaga acccggcagg aatgcgcgga tgctgctaat cataacggtc aggccgcctt
     4621 tgactttgcc gttgatgatg ccggacagga tgttgccgtt ttccatggcc tcttccaggg
     4681 caatccaatc ggcagcgcgt ttggcttttt cgcgggacag tttggtttcg ccgaagccgt
     4741 tttcgacgga ttcgatggta acggtaacga agtcgccgac tttaacttca atttcgcctt
     4801 gagcgttttt gaattcagct acatcaatca gggattctga tttcagacct gcgtttacgg
     4861 taacgaagtt ttggtcgatt gccactactt cagcggtaat cacctcaccc gggttcattt
     4921 cttgcagggt aaagctttct tccaacagct gagcaaaatt ttccatagac atatataact
     4981 cttttcggta caccgccaag gggtgcgggg tgggttggtg gatatctgcc gtccttggca
     5041 gggcagacgg gtacataagg tttcagacgg catatggggg atttttatgc cgtctgaaac
     5101 gtaatttgtg cgattatacc tgaaaattta aacttcacga taccaatcaa gcactttttt
     5161 tacagtttct tctatagtca ggcggcttgt gtccaaaagc agggcatcgg gctgttgttt
     5221 caggggggca actttgcggt ttcggtctgc ctcgtctctg gcttcgatgt cggacaggat
     5281 gcgctcgaat gccaaacctt cgcaggggat gccgatttgt ttggcgcggc gttcggccct
     5341 gattttggat tctgctgtca gaaaaatttt gagttcggcc tgggggaaga cgaccgatcc
     5401 ggtatcccgt ccgtcggcaa ccagtccttt ttcggtcaga aaatcgcgtt ggcgttgcag
     5461 cagggcggcg cggactttag gcaactgtgc gactgcggat gcgcccatgc cgatggcttc
     5521 tgtccggatg ccgtctgaaa cgtcttcgcc gccgagcagg atgcggctgc ctgaaaatac
     5581 ggcgggcagt ttttttgcca gttcggaaac gttttcttca tcgtgccatc ccacgccttg
     5641 tttttgtgca tatagggcag tcaggcggta gagtgcgccg gtatcgagat aatcgtatcc
     5701 caatgcggcg gcgacgcggg cggcgaccgt gcctttgccc gatgcgcccg ggccgtcgat
     5761 ggcgatgact ttttgtctgt tcataatggg gattcctgat ggtttggggt atgggttttg
     5821 ccgtctgaag gatgtgtttc ccgttggggc ggattctacc tgttttaaag gacgattgtc
     5881 taagcagacg gaacgccgcc tgcccggaaa tatccgacag aagccggcaa gccggttcgg
     5941 attccggcgt tgcgggtatt gtggcggaca tcaatgcaat gccgtctgaa agaggtatga
     6001 ccttcagacg gcatcgattg ctgcggatta aaacaggttg ctgacgaaaa tcagcaaaat
     6061 caccagcggc acgaggtatt tcacataggc aaaccaaata ttgaccgtcg tatggttgcc
     6121 tttataaagc aattcttcct tcgcttcatc cttcatcacg aaaccgacaa acaatgccga
     6181 gccaagcgcg gtcagcataa acaagatgtt gccgctgatg tagtcgaagg catcgaaaat
     6241 atttttgccg aacacggaaa cgtctttcca cgggccatag ctcaaaatag acgggatgtt
     6301 gccgaaaacg tagatggcgg ccaatacaat cgtgattgcg gcggtacggc ggattttggt
     6361 tttttcctga atggtcgtaa tcaacacttc ataaatcgtc agcgaagttg tcagagcggc
     6421 aatcaggagc agagagaaga aaatcacggc gaacacaggt cccgcccaca tatgtgagaa
     6481 cacaatcggc aagctttgga acaccaaagt cggaccggaa tcgggggcaa cgccgaagct
     6541 gaagagcgac gggaaaatca taaagcccgc aagtacggcg atgatggtat tggtaattgc
     6601 cgtgataact gcggtttgca ccagattttc gtttttatcc aaatagctgg acaaggtaat
     6661 catcacgccg aaacccaggc tcagggcaaa aaatacctgc cccaaaacga agacgaacag
     6721 ttcggcggta atcttgctga aatcaggttt cagatagaaa gcaacccctt ccattgcgcc
     6781 cggaagggta acgttgcgga cgaccatcgc gattaggaac aaaaacagca gcggcatcag
     6841 ctattttgcc gctttttcaa tgccgccgat aacgcctttg accaaaatcc attggttcac
     6901 ggcgacaaaa agcagcgtat aaaacgcaat ttcccaaggg ctgtttcaat gtgttcggca
     6961 aagaagcctt ttgtaaccac accgtcgatg gggctggaaa tattcaaatt tcctccaata
     7021 atattaacga tatagctgat tacccagccg ccgagtacca tgtaataagc catgatgccg
     7081 aacgtgccga gcagccccat ccagccgacc agtttccaaa ttttagcaat gggtttgccg
     7141 ttcatcggac cgccgaacgc atccagcgcg ttcacgcctt tgcgccgtcc gatgacattt
     7201 tccaccaaaa tcatcggaat gccgataacc agcatcgcga tacagaataa aaacacatac
     7261 gcgccaccac cgttttcacc gaccaaatac gggaaacgcc acgtcgcgcc gaaaccgaca
     7321 gtcgcgccgg caacggtcag gatataggtt aatcggctgg accaagtttg acgattggta
     7381 tttgaagggg aagacatatt gaaaccgtgt ccgattgaga taaagcggaa attctacacg
     7441 cgttttttaa caggaactga ctgattgctt ttcaactgtc tagtgatttt ccatgtaaaa
     7501 ggcatatatt tcagtgagta tttctgatga gtataacccg atgaggaaga atcggagttt
     7561 ataaatagat taatttgtta ttcttctaca tcggtgtatg gaaatgaaat tttgttaatt
     7621 atattaatga tagcatttaa ataatgatga aagagaggga atttgaaata tagtggatta
     7681 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7741 caacgctgta ctggtttaaa tttaatccac tataaaaaaa ccatctatac aaggggagaa
     7801 gtatagatgg caaaacacat tacggggaaa acgtcttact cataagcctg cttgaacagg
     7861 cgttactcag acaaatggat tatatcgtaa attgattttt tgcgttaaat tgggttaaac
     7921 catatattta atgagtatgt ggatgaatat aaatggaatt cttggatgtt atttcagaat
     7981 tttggacatt cgataaattt ccattccccg ggtttgaggt tttctgtttc caaatgtgcg
     8041 aatcgccgac gatgaaggtg tagcacgcgg ttgccggcgg cggcgatcat gcgtttgact
     8101 tggtggtatt ttccttcggt aatggtcagc agcagggtgg tcgggttttc caaaacggca
     8161 tcggcggcac aaacggtttc gttttcgtcg tggagcagca tgccgttttt caaggtttcg
     8221 cagagcgttt ctcctgtggg gtgtttgagc gttacttcgt atagcttggg aatttttctg
     8281 ctcggcgaag tcaggttgtg gttcagtttg ccgtcgttgg taatcagcaa tacgccggtc
     8341 gtatccgcat ccagcctgcc gaccgcctgc atatcgatgt tccgcatatt gtcggggaac
     8401 aggctgaata cgctgcggta gtgcttgggt ttgtgcgaag tttcgtaatc ttcaggcttg
     8461 ttgagcatga tgtagaaata gggttcggga acgacggtta ctgcttcccc gtcaatatcc
     8521 aacgtttcga cggatgagga atcgatgtct gcatcggtgt cgtccatgca ggttccgttg
     8581 atgaaaacat aaccgccagc aatcagccat tggcactgct tgcggcttcc tatgccttga
     8641 tattgcaggt atttgatgag tttcatgatg gtatgggaaa tgtgggatga aaaagacggg
     8701 actgagaaag tcctgtcatg tgccgtatcc gaatcagctt ccaatcagtt tgaccctctg
     8761 tcccggattg atggtgttca ggttgggatt gagccggcgg atgtcgtcga tatggatatt
     8821 gaagcgcgcg gcgatacttt tgaaggtgtc gcctttgcgc gcggtgtagg atactttttc
     8881 aatacgggtt tgggcagggg ctgcctgcgc caggcggagt acctgtcctt tttggatggt
     8941 gttgcctttg atgttgttgg cgacaatcag gtcggctacg ctgacgttgt agcgtttggc
     9001 aatgttgaac agggtgtcgc cttctacaac gcggtggatg ctggcatgga gcggggagat
     9061 ttcggcttcg ccgtcggcag ctttggctac gcgcgtttcc aaacgtgccc ggcgtttgcc
     9121 gtcggcggca acggtatgtt gcggagatgc ggaaatgttg tgttcggcaa ctgtgtcagg
     9181 cgtgccgatg acggcggaga tggtttcttc agcctgtcgg cgcaggttgt ttcgggcaac
     9241 aagctgcatg agttcgtctg ccgcctgcat gtcgttttgg gggatgatct gcgcgagtgt
     9301 tgcggtttgg gtttcagacg gcatggcggt ctgtttttcg gtttgaggtt gcgctgcggc
     9361 tgtcgcaagg gcaggttcgg caatacggac aaggggatcg ggttccgtac ggacggtttt
     9421 ctgcggcaaa ggtgcgacgg taatgtccgc tgtctgtgcg gcggcgggtc ggattcgggc
     9481 aatgccgacg ttcaccgtgc ctgccggcat attggaacgg taggtgtcgg gcgtattgtc
     9541 gatgtcgatg gaaacgacgg attccgatgc cgtctgaagg gttttgccgt tcttggcgac
     9601 aaggatgctg cgtcctgcgt tgacaaggtt gccgttcagg ttgttgaggc gtttgatgtc
     9661 ggcaatgctc atgccggttg ccgtcgagat gtcggacagg ctggttttgg cggcaggcgt
     9721 atagacttcc catgaaaaca ggctgtcggg tgcggcgttg aggtagttgc tttggaaggt
     9781 ttggacggat gcgacaggaa gcagcagttt gcgtttgctt ttggggatga acgcggggac
     9841 gttgaatgcg gggtttaggg cgagcagctc gctttgcgtg atgccggcaa gccgggcgat
     9901 ggcttcgttg tcgagcggac ggtccggttc gattgcctga aaatagggtt tattgtctat
     9961 gtcgctgata ttcatgccga aagattgggg ggcggcaatg atgttgcgca cggcgagcag
    10021 cttgggaaca tagttgcgcg tttcgttggg catacgcagg ttttcgtagg tcggttcgag
    10081 cccttgggcg cgggcgcggt tgatggcgcg tccgacgttg ccttcacccc agttgtaggc
    10141 ggcaaaggcg agcggccagt cgccgaacag tccatagagg tattgcaggt agttgagtgc
    10201 ggcatcggtg gcggcgtaaa cgtcgtgcct gccgtcgtaa accggtgttt tttccaagcc
    10261 gtaatgcctg ccggtagcgg gcataaactg ccacaggccc gatgcgccga catgcgattt
    10321 ggctttggtg acgaacgcgc tttcgatgaa gggaagcagg gcggcttcgg cgggcatatt
    10381 gcgttttttg acttcattgg caatatggta catatagggt cggctccggt tgacgaccct
    10441 gtcgaaatag ctgcggcttg cgatgaattt actttcgtgg cggcgtacca gttcgggatt
    10501 gacttcgccc atctggaagc cttggcgcag ctcgccccac aggctgccgg attggaaata
    10561 ttgttttgtc gggtgcaggt cgagtattga agagtttaag cgcataatcg ccaaaccgat
    10621 ttggtgtgaa gaggtgtttt gagcgtatag tgagccggaa cagacggaga gacccgaggc
    10681 agtcaggacg atggttttga gtttggacat gatttatatt cggcggaata aacatatttt
    10741 atcgtccgat ggtactgttt gactgaaaaa gcgtcaagtt ttacaggaac tttatgcgga
    10801 ttcggaaatg ggcggccgat gtgttttttg acagaaatcc tgtttggaat agaatatcgg
    10861 caggcaatct gtttgaaacg gacgcaatat ggatgcatgg tttgaagata cggcgatggg
    10921 gcggtatgtt gcaaaattgg aacaggattt cttcgggcgg tatctggatt cataccgttt
    10981 ttcgggaatg tgtgcggttc aggtgggcgg tccgtggctg agcctgtctg aagatgttgt
    11041 ctgtgtgccg cgagatatgt cgatgtcggc ggagaatatg gctttggcgg atgtttctgc
    11101 ggatatgctg ctttttccgc atacgcttga aggcggtgtt tcttcgcaaa tcctgtcgga
    11161 agcgcaccgg atattgaaac cgtgcgggcg cttgatgctg acgggtttca atccgtattc
    11221 gctctgggga ttcagccgtt ggtttgacgg cgaacgcctg ccggaaaaac ggttttgtct
    11281 gccgttgccc gagttgaaaa ggcggcttgc cgatgccggt ttcgatattg aatttgggaa
    11341 atttatggtg tatctgccgc ccgtttcgtc gctcgggcaa atacgctttt ggcggtttat
    11401 ggaaaaggcg ggcgaccgtt ggtggccgca atgtgccgca gtgtacggtt tggttttggt
    11461 caaaagggcg gcgggcgtaa cgcccctgcc cgagtgggac gggtatttgg gcggcaaagc
    11521 ccttgcggcg ggtgcggcaa gggttgcgga ttagggacag ccgtctttca gacggcatct
    11581 gtcagaagaa atgtcgggag gggttatgcc tgaattgccg gaagtggaaa cgacgttgcg
    11641 cggcatcgcg ccgcatattg aagggaaaac ggtggaagcc gtggtattgc gccaattgaa
    11701 gctgcgctgg cagattaatc ccgatttggg ggagattttg tccggtcggc aggttttgtc
    11761 ctgcggcagg cgggcgaagt atctgattgt ccgtttccaa acggggattt tgctgattca
    11821 cttggggatg tcgggcagct tgcggatttt tacgccgtcg gacggacgta tcggcaggcc
    11881 ggacagacac gatcacgtcg atattgtgtt ttcagacggc acggtcatgc gttaccgcga
    11941 tccgcgaaag ttcggcgcga tactttggta tgagggaatt gaagagcatc atccgctgct
    12001 ggaaaaactg gggccggagc ctttgtcgga ggcattttgt gcggattatc tgtatgcaag
    12061 gctgaaggcg cagaagcgcg cggtcaaact tgccctgatg gacaatgcgg tcgtggtcgg
    12121 tgtgggcaat atttatgcca acgagagcct gtttagggcg ggcatttcgc cccaccgtcc
    12181 tgccaaccgc ctgaaaaaga aagagtgtgc gcttttggtt gaaaccgtca aagcggtgtt
    12241 gcggcgcgcc attgaaacgg gcggcagtac gctgagggac tttgtggaca gcgacggcaa
    12301 aagcggctac ttccaacagg aatatacggt gtacgggcgg cacaatcagc cgtgccccca
    12361 gtgcggcggt ttggttgtga aagaaacttt ggggcagcgc ggcacgtttt actgcccgaa
    12421 ttgtcagaaa tagggctgaa aacggtttca gacggcattt tatcggtatg ccgtccgaac
    12481 gtttcaacaa caaacaccga ttatcgggaa agaattgttc atgtcttcaa ataaagcttc
    12541 attttttaca cgtctgcgcc gcttgtgccg cttgacggtc tggctattca aaaccggcaa
    12601 aaacctgcgc ggtattgacg gcggctgccc cgagtcgcgc aatcgggcgg taatcgagtt
    12661 gggcaggggg gttttggcgg ctttggatat cggattggag gtgggcagac ccgcacccga
    12721 acatccgaac ggtgtcttgg ttgccgccaa ccacgtgtcc tggctggata ttttcgcgat
    12781 gagcgcggtt tatccgagca gctttatcgc caagcaggaa atcaaaagct ggccggtatt
    12841 gggcaagatg gggcagaacg cgggaacggt gttcatcaac cgcaattcgc ggcgcgacat
    12901 cgaaccgatt aaccgcgccg tctgcgaaac cttgcaacgc ggtcaaaacg tcagtttttt
    12961 ccccgaagcg cggacttcct ccggattggg gcttttgccg ttcaaagccg cgctgttcca
    13021 atccgccatc gatgcggggg caaaggtttt ggcggtcgcg ctgcgttatt atgacgaaac
    13081 gggaaaaagg acggctcgtc cctcatatgc cgatgtcggt ttgccgacct gcctgtggcg
    13141 catcgtgtct atgaaaaaat tgacgataaa agtcgatttc gtttgcgtgg cggatgcggc
    13201 ggaaagcgaa gaccgttatg ctttaaaaga taaaatcgaa gaaagcatcc gtgccgttgt
    13261 cgccgacgat gcggatatcg ctgtctgaaa ccggttgtcg gaatgtggca gtatgattcg
    13321 cttttgtgga tgtatagtgg attaacaaaa accagtacag cgttgcctcg ccttagctca
    13381 aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggtttcgta ctatctgtac
    13441 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatta aggctgcagc
    13501 aacgggagta cctcaaacga aatggcgtaa ggatggtttg ttgtatctat ggaaaatgcc
    13561 gtctgaacct gtgttcagac ggcatatttt atatgggatt aacggcggaa actgccgccg
    13621 tcgaaatcat cgaaagcctc ataagccgcc cggcattgga ttttctgttc cgcataaggc
    13681 agggagcgga atgctttgcg cgcctttttg ttttcgcgca attcctgcac gttgccttgc
    13741 gatcggtacc acgcggcacg gcgttccaaa tatttcctgc attcggggtg cagcatctgc
    13801 ttgtttgcct tgcggatatg tttggtatcg cggtgggcgg gttctgacgt accggcagcg
    13861 gcagttgtcg ataccagtcc caatattgca gacaacagta tggcaagcat ctttttgaca
    13921 gacatgacga tcctttcagt ttgtcattcc ttccagtata ggatggaagg atggaacggg
    13981 caaattgtcg gcagtttcag actcattcag ccgacaaaac ttgccgtttg atatgcggat
    14041 tcaaataaca ca
//
LOCUS       DAXIEB010000050        13769 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000050 DAXIEB010000000
VERSION     DAXIEB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.050"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(259..681)
                     /locus_tag="ACFOA3_001166"
     CDS             complement(259..681)
                     /locus_tag="ACFOA3_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6898176.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEE
                     RYKLSRSEFEQLWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
     gene            complement(824..2470)
                     /gene="pgi"
                     /locus_tag="ACFOA3_001167"
     CDS             complement(824..2470)
                     /gene="pgi"
                     /locus_tag="ACFOA3_001167"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6898177.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            complement(2591..3439)
                     /gene="hexR"
                     /locus_tag="ACFOA3_001168"
     CDS             complement(2591..3439)
                     /gene="hexR"
                     /locus_tag="ACFOA3_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6898178.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3489..4475)
                     /locus_tag="ACFOA3_001169"
     CDS             complement(3489..4475)
                     /locus_tag="ACFOA3_001169"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6898179.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4456..5151)
                     /gene="pgl"
                     /locus_tag="ACFOA3_001170"
     CDS             complement(4456..5151)
                     /gene="pgl"
                     /locus_tag="ACFOA3_001170"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6898180.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5431..6876)
                     /gene="zwf"
                     /locus_tag="ACFOA3_001171"
     CDS             complement(5431..6876)
                     /gene="zwf"
                     /locus_tag="ACFOA3_001171"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6898181.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7392..9227
                     /gene="edd"
                     /locus_tag="ACFOA3_001172"
     CDS             7392..9227
                     /gene="edd"
                     /locus_tag="ACFOA3_001172"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6898182.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9408..10046
                     /locus_tag="ACFOA3_001173"
     CDS             9408..10046
                     /locus_tag="ACFOA3_001173"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6898183.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10061..10279
                     /locus_tag="ACFOA3_001174"
     CDS             10061..10279
                     /locus_tag="ACFOA3_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898184.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(10303..11344)
                     /locus_tag="ACFOA3_001175"
                     /pseudo
     CDS             complement(10303..11344)
                     /locus_tag="ACFOA3_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11507..12547
                     /gene="mutY"
                     /locus_tag="ACFOA3_001176"
     CDS             11507..12547
                     /gene="mutY"
                     /locus_tag="ACFOA3_001176"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6898185.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     SELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            12964..13524
                     /gene="sodC"
                     /locus_tag="ACFOA3_001177"
     CDS             12964..13524
                     /gene="sodC"
                     /locus_tag="ACFOA3_001177"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6898186.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3203 a         3724 c         3475 g         3367 t
ORIGIN      
        1 gtatcggttt aaatttaatt cactataaaa acgacaaaaa cgcaaaagcc gccgacattc
       61 ccgcatccaa gtttcagtca atcagataac cttggatttc tttggttttc gcattgattt
      121 ctctggtacg gcagtcagat tgtgccacgc cgtattcgtc gccgtcggcg catttggcat
      181 tcaaaccgtt ttgtcagttg cggtactcgg gcatgacggt ttcggcaata tcggcgggca
      241 gtgcctgaac cgcgctgttc agagccgctt tggcagttct ttcttgtccg cttccctgac
      301 catttcgtcg ataagggctt ttttacggtt gtacagctct tccaagcgtg cttcggtttc
      361 caccgttttg catgccagtt cgctgacttt tatgccgttt ggcgtttcac cttcggcttt
      421 cgcattgttg gcacagattt tgtccatacc gcttttccat gttttctgtg aggcttgcag
      481 cttattctgt acggtttgag gcaatccttt ccagagctgc tcgaactcgc tgcgcgacag
      541 cttgtagcgt tcttcccatt cggatacgcg gtcgacttcc tgctcccgac ccaatgcctc
      601 ctgcgccgcc gcttcttctg ccgcttcaag ttcttcgttc ctttgtttcg ctttgtccaa
      661 cggctcttta atcagtgcca tagctgcgga acatatatgt ttaaatttat gcaaaccatc
      721 atatcgggat tgcacacgct ctgcaagttt accgacggtt ttcctgttcg ataaaaatgc
      781 cgtttgaaac ggtcggcgtt cagacggcat ttttccgcag gttttatttg cggttggtct
      841 gcaggtagag gttgatcagg cgttcggtcg agctgtcgtg cttttgcgac ccggtttcgc
      901 cggtcagttc gcccaaaatg gttttagcca gttgtttgcc gagttccacg ccccactggt
      961 cgaagctgtt gatgccccaa atgatgcctt gtacgaaggt tttgtgttcg tacatggcaa
     1021 tcaggctgcc catattacgc gggttgacct tgtccatcag gatgaggttg gtcgggcggt
     1081 taccggagaa ggttttgtgc gggaccagct cttcgatgcg cgcctcatcc ataccctgcg
     1141 ctttgagttc ggcgcggact tcgtcggggg ttttgccgcg cataaaggct tctgcttggg
     1201 cgaagacgtt ggcaagcagg atttcgtggt gtccgggcag gttgctgcgt ttttcaagcg
     1261 aggcaatcag gtcgatgggg gtaatgtgcg tgccttggtg cagcagttgg aagaaggcgt
     1321 gctggccgtt aatgcccgtt tcgccccaga taatcggcga ggtttcgtgt ccgactgctt
     1381 tgccgtccaa cgtaacctgt ttgccgttac tttccatatc gagctgctgg atgaatttgg
     1441 gcaggcggtg caaatgttgg tcgtaaggcg cgatgacgtg gctgccgccg ccgtagtagt
     1501 tgatatacca gatgccgatg agggcgagaa tgacgggcag gttgcgctcg agcggtgtgt
     1561 tgatgaagtg ttggtccatc aggtgcgcgc cgttgagcat ttcaatgaag ttttcttcgc
     1621 cgagatacag cataatcggc aatccgatgg cggaccacag gctgtaccga ccgccgaccc
     1681 aatcccaaaa ttcaaacata ttggcggtgt cgatgccgaa ttcggcgacg gctttttgat
     1741 tggtggaaac ggcggcgaag tgtttggcaa cggcttcttc gtcgcccgca tgattcaaaa
     1801 accattcgcg cgcggtcagc gcgttggtca gcgtttcctg cgtggtaaat gttttggagg
     1861 cgatgatgaa cagcgtggtt tcggggtgga ctttggacaa tacgtcgcgc agttgggagc
     1921 cgtccacgtt ggagacgaag tgcatattga ggcgcggatg accgaaaggt ttgagcgcgg
     1981 tacacatcat cagcgggccc aaatccgatc cgccgatgcc gatgttgaca acgtcggtaa
     2041 tgacttggtt ggtatagccc agccagcttc cgctgcggac ttcgtgtgca aattcgccca
     2101 tacgttgcaa aacgcggttg actttgggca tcacatcttc accgtcaacc acaatcggcg
     2161 aattggtgcg gttgcgaagg gcgacatgca ggacggcgcg gttttcggtg gtattgattt
     2221 tttcgccgtg gaacatctgc cgcatccgct ccggcacgcc tgcttctcgg gcaagctcga
     2281 acaaaagcga catggtttcg tcgttgatgc ggtttttgga gtagtccagc gtcagtccgc
     2341 cgacttgcag ccagtagcgt tccgcacgct gcgggtcttg ctcgaacatt tcgcgcatat
     2401 gcaatgtttt gctgtcgtca aagtggttcc acaatttcga ccatgcgggt aagtcgtgaa
     2461 agtgtttcat ctatatgctc ctgaatgagg ttttttgttg tgggatgaaa aggctgctgg
     2521 aaactgccgc aagccgccga cggccgttgt tcggcatttc agacggcatt tgtgggatgc
     2581 cgtctgaagg tcaatctttg tcgtaatcga tgtgcttgtt gtgtatgctt tttttgcttt
     2641 tctgcaattg caggctggca gcatcgccca agcgcagggc aagtccgatg gcgagaatgt
     2701 cgatgacggc aagctgcaag aggcgggaaa ccatgggcgt gtagagttcg gcattttcct
     2761 gtgtggcaac gctcaacacg cagtcggcaa gttgcgccag aggcgaatcg ttgcgggtca
     2821 gtgcgatgac ggacgcgccg ttttctttgg cgatgctgac cgcatccaaa agttcgatag
     2881 acgaacccgt gttggaaatg gcaaccaaaa catcctgatc gctcaaaacg gatgctgcca
     2941 tcagctgcgt gtgcgtatcg acataggcga ccgtggacat tccgaaacgg aaaaacttgt
     3001 gttgcgcgtc ctgcgcaacg ataccggaat tgccgacacc gtaaaactcg acgcggcggg
     3061 cgtgcatcag cgtggcaatg gcgttttcca gctccgactc tttcaggaag cggcgttcgc
     3121 ccaacagcga ggcggcggca ttgcccaaca ctttctcgac cacgcttgcc atatcgtcgt
     3181 cggcgttgag ttcttcatgg acatagggca taccctcatg accgatgctg gcggacaacg
     3241 cgagcttgaa ctcgggcagc cctttataac ccaagctgcg gcagaatcgg atgacggtcg
     3301 gctggctgac ggacgcacgt tcggcaattt cggcaacggc ggcatggacg aaccatttgg
     3361 gttccgccaa tgcacattcg gcgactttgc gttccgcacc ggaaaggttt gccaatgatt
     3421 cgctgatttt gcttaacata atgatatgcc cttcgataat gcagccccgc tgcaaggagc
     3481 cgctgtggtt aaacgtttct caaatggttg tcgagagccg cagccgcacc agaaattccg
     3541 ggaaactcgc tcaagacgac atacacggga atcgcggcaa gatatgcttc aaacctgccc
     3601 ttgttctcga aacggctgcg gaacggggaa gttttgaaat attccaacac gcggggaata
     3661 atgccgccac acaggtacac gccgccgcgc gcgcccagcg tcagggcgag gttggaagca
     3721 accgtgccga gcatggcgca gaagatgtcc aaagtctgac ggcacaaagg cgacgcgccg
     3781 ctcaaagcct tttccgtgat ttcagacggc atcagtttgg cgggtttggc tttctgtttt
     3841 gcagccaaag cctcgtaaac caagctcaag cccgcgccgc tcaaaaagcg ttcggcggaa
     3901 acatggccgt atttgttttt ggcgtactgc caaatcagca cttccatatc gtcaaacggc
     3961 gggaaactgg tatgcccgcc ctcgcccgcc aaagccaccc agcctgcgtg gctgtgcacc
     4021 aatccgctca cgcccaggcc ggtaccgggg ccgataacgg ctttgggggc aaattcgaca
     4081 ggcttttgcc cgcctacctg catcaggtct ttgcttgaag tctgcgttac cgccaatgcc
     4141 tgcgcggtaa agtcgttcaa aaggatgagg gtgtccagcc ccaaagtctg acgggtggtt
     4201 tcgatggaaa acgcccaatg gtggttggtc atctgcaccc agtcgcccaa aatcgggttg
     4261 gcgatggcaa atgccgcgtg ccgtacggct gttgcaccgc tttgattcag ataggcacgc
     4321 accgcatcgg taaccgtatc gtagtcttta cacggaagca cggcggcttt ttcaatgacg
     4381 cgcggcgcgg tttccagcgc aaagcgtgca ttcgtcccgc cgatatcggc gaccagtcgg
     4441 ggatatccgg cttgtttatt cggcgtagaa gacatggcag ttcactcctt gatggttcaa
     4501 aacgaggttg atcggatatt cgcggttttc gccttgtgcg gcttggtcga acacggcttt
     4561 tttctcttcg ccccgtatcg ccaaaaacac atgccccgta tgggcaatcg catccaaggt
     4621 catactgacg cgctcgtgcg gcgcggtaac gggcgtggta tgcaccagcg cgacacctgc
     4681 cgaaccgtcg attgccgtct gaaactgcgg agctttcggg aaaatcgaag ccgtatgccc
     4741 gtcgtttccc atacccaaaa ccaaaacatc gggctgtttg taatgtttca gtgcataatc
     4801 gacaacagca tcgggatgta attcggtttc agtttttccg tcttccacca taggaatcca
     4861 cattgccgct tccgctttgt tcttcaacag gtattcgcgc accaaaccgg tattgctgtc
     4921 ggcgtggacg gtcggcacga tgcgttcatc tgccaaggtg atgccgacgt ttttccaatc
     4981 caaatctttt tgcgacaggg cgttgaaaaa tgcaatcggc gaacgtccgc cggaaactgc
     5041 caacaccgca ccgcccttct cgtccagtgc gccctgcaaa gcatccgcca ctgcgtcagc
     5101 caaagactgc gccgcttctg ccgcattttc gtattcgtgc caaacaaaca tatttgtgtc
     5161 ctttttttat ttcagacggc atattccgtt atggaaacgg gttgagcaat atgccggtcg
     5221 aacagttgtt tatgcttttg ataccaaata tcgggactgc tttttatagt ggattaaatt
     5281 taaaccagta cagcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     5341 ccttgtcctg atttttgtta atccactata ctgctttact tgcgttcaga cggcatttca
     5401 aaccccatgc cgtctgaacg cattattgta ttactgctct tcgtgccact tgtgtccgtc
     5461 gcgcgccaat agttcgcgcg cggcttcagg cccccacgag tgtgcgccgt agccgtgcgg
     5521 cggcgtggtg ttatttgccc agttttccaa aatcggcatc acatattccc acgcggcttc
     5581 gagttcgtcg cggcggttga acaaagcgag tttgccgtta atcacatcca gcagcaggcg
     5641 ctcgtaagct tccgcgcggc ggccttccaa tgctttgccc aaatcggttg ccagcggcac
     5701 ggtttcgacc ttatttcctg cccccggggt tttcatctgc gtatagaggc gcacggattc
     5761 atatggttgc aactcgataa cgagccggtt gggcgcggtg cgactgcctt caaaaatatg
     5821 gctgttcaaa tctttgaagt tcaaaacgat ttccgccact ttgcccgcca tgcgcttgcc
     5881 ggtacgcagg tagaagggaa cgcccttcca gcgttcgttt tcgatttcgg ctttaatggc
     5941 gacgtaggtt tcggtaaagc tgtcttgcgg aacgttgatt tcttcaagat agccgttcat
     6001 gcctttggcg gcggtatatt gtccgcgcac gacattttca ttgacagatt cgacggtcag
     6061 cggcttcaat gacttgatga ctttgacttt ttcatcgcgc accgcgtcgg catccaagct
     6121 ggcgggggct tccatcgcag tcatgcacag catctgcatc aaatggtttt gcaccatatc
     6181 gcgcaacgcg ccggtaatgt cgtaaaactc gccgcgctct tccacaccga gctgttcggc
     6241 gatggtcaac tgcacgcttt cgatgtattt attgttccac agcggctcga acattacatt
     6301 ggcaaaacgc agcgcaagca ggttttgcag gctttctttg ccaaggtagt ggtcgatgcg
     6361 gtaaatttgc ccttctttga aataacgcgc aacatcggta ttgatttgct gggaagaagc
     6421 caaatccgta cccaacggtt tttccaaaac cacacgcacg ttgtcggcgt tcaaaccgat
     6481 tttggcaagg ttttcacatg tttgcgcgaa gaatttcggc gcggtggaaa gatagatgac
     6541 gacattgtcg gtttctttgc gcgctttgac caaatcgccc aaagcggcaa aatcgtccgg
     6601 ctgcgtaaca tcgactttga gatatgcgag acgttcgaca aacgatgccc aagcctcatc
     6661 ggaaaaattt tctttcacat ggattttgga actggtttcc accttcgcca gaaaaccttc
     6721 ggtatccaac tcgctgcggc tgacccctaa aatacgccct tcgggatgaa gcagaccggc
     6781 aacatgcgcc tggtacagac agggcaacag cttgcgcatc gccaaatcgc cggtcgcacc
     6841 gaacaacacc aaatcaaaat ttgtttgtgt actcatcgta ttatctcgtc aggaaagaat
     6901 ttttcgatgc cgtctgaaac ctgtttcccc catcacgctg catcgcaata tcggaaacaa
     6961 aggcaggcgg cataatgagt agtaatacta cacaccgcta cactttttgt ctattcccat
     7021 ttttacaatt tatttgacct agtccaaaaa tcgggcaggt ttcccctatt ccgttacaac
     7081 aatcggaaga ttctgcgatt taaatcaaat ttcttttcga tgtctgattt ttttgtaaca
     7141 aaattacaaa ttttgtacta taatagcacc cgcttcccac tttcagacgg catacctttt
     7201 aaaatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata
     7261 atacggcaag gcgaggcaac gccgtactgg tttttgttaa tccactatac tcaccgtctg
     7321 aatacccgat acaaaaatca gaaacgcaca aacaaatccc caataccccc cgttccgaca
     7381 ggagaccgac cgtgaacact actcctatcc actccaaact cgccgaaatc accgggcgca
     7441 ttattgaacg cagccgtccg acgcgtgaaa aatatctggc gaagatccgc agtgccaaac
     7501 aaatgggacg cttagagcgc aaccagctcg gttgctccaa cttggcgcac ggctatgctg
     7561 ccatgcctaa aagtatcaaa atcgaaatgc ttcaggaaac cgtccccaac ttaggcatca
     7621 tcaccgccta caacgacatg gtttccgcac accagccgtt taaagacttc cctgaccaaa
     7681 tcaaagacga agcgcagaaa aacggcgcga ccgcccaagt cgccggcggc acacccgcca
     7741 tgtgcgacgg catcacgcaa ggctacgccg gtatggaact gtcgctgttc tcccgcgacg
     7801 tgattgccat gagtaccgcc gtcgggctgt cgcatcaaat gtttgacggc agcctgttta
     7861 tgggcgtatg cgacaaaatc gttccgggtt tgatgatagg cgcgctttcg ttcggtcata
     7921 ttccgggtat cttcgtcccc gcaggcccga tgtccagcgg tatcggcaac aaagaaaaag
     7981 cccgcacccg ccagcttttc gccgaaggca aggtcggacg caacgaactt ttgaaaagcg
     8041 aaatgggttc ttaccacagc ccgggcacct gcactttcta cggcacggca aactccaacc
     8101 aaatgatgat ggaaatgatg ggcgtgcacc tgcctgccgc cgccttcgtc cacccttaca
     8161 ccgacctgcg cgaagcccta acccgctacg ccgccggaca cctcgcgcgc ggcatcaaaa
     8221 acggcacgat taaacctttg ggcgaaatgt tgaccgaaaa atcctttatc aacgccttga
     8281 ttggcctgat ggcaaccggc ggttcgacca accacaccat gcacctcgtt gccatggcac
     8341 gcgccgccgg cgtgattttg aactgggacg acttcgacga aatttcctcc atcatcccgc
     8401 tgctcattcg cgtttatccg aacggcaagg ccgacgtgaa ccactttacc gcagcgggcg
     8461 gactgccttt cgttatccgc gaattgctga atgcaggcct gttgcacgac gatgtcgata
     8521 ccgtcgtcgg acacggtatg cgccactaca ccaaagagcc tttcctcatc gacggcaaac
     8581 tcgaatggcg cgaagccccc gaaaccagcg gcaacgacga catcctgcgt aaagctgaca
     8641 acccgttctc ccccgacggc ggtctgcgcc tgatgaaagg caacatcgga cgtggcgtga
     8701 ttaaagtgtc cgccgtgcgc gaaggctgcc gcattattga agcgcctgcc atcgtgttca
     8761 acgaccaacg cgaagtgttg gctgcgtttg aacgcggcga gttggaacgc gattttgtgt
     8821 gcgtcgtccg ctaccaaggc ccgcgtgcca acggtatgcc cgaattgcac aaactgaccc
     8881 cgcctttagg catcctgcaa gaccgcggct tcaaagtggc gctgctgacc gacggccgta
     8941 tgtccggcgc gtccggcaaa gttccagcct ccatccacat gacacccgaa gccctgatgg
     9001 gcggcaacat cgccaaaatc cgtaccggcg acctgatccg cttcgactcc gttaccggcg
     9061 aactcaacgt cctgattaac gaagccgaat ggaacgcccg agaagtcgaa agcatcgact
     9121 tgggcgcgaa ccaacaaggc tgcggccgcg aacttttcgc caacttccgc agcatgacca
     9181 gcagcgccga aaccggcgcg atgagcttcg gcggcgaatt tgcctgatgc gcgtttcaga
     9241 cggccttttc agaccgaagg ccgtctgaaa aattattcaa gtgttttaag atagacgtag
     9301 gtcggattct cgaatccgac acagccgtcc aagatgtcgg tttcttgaat ccgacctaca
     9361 acctgtccca tcttaataaa ataccccatc ccacccggag aaccgaaatg tccaaactga
     9421 ccccccgcga aatcctgacc gccggcgcag ttgtaccggt aatggcgatt gacgacttga
     9481 gtactgccgt cgatttgtcc cacgcccttg tcgaaggcgg catccccacc ctcgaaatca
     9541 ccctgcgcac ccctgtcggc ctcgatgcca tccgcctgat tgccaaagaa gtgcccaacg
     9601 ccatcgtcgg cgcgggtacg gttaccaacc ctgaacagct caaagccgtc gaagacgcag
     9661 gcgcggtttt cgccatcagc ccgggtttgc acgaatccct cgccaaagcc ggccacaaca
     9721 gcggcatccc cctgattccc ggtgttgcca ccccgggcga aatccaactg gctttggaac
     9781 acggcatcga caccctcaaa ctcttccctg ccgaagtcgt tggcggcaaa gccatgctca
     9841 aagccctgta cggcccttac gccgatgttc gcttctgccc gacaggcggc atcagcctcg
     9901 ccaccgcgcc cgattacttg gcactgccca acgtcctgtg cgtcggcggc tcatggctga
     9961 cgccgaaaga agccgtgaaa aacaaagact gggacaccat cacccgtctc gccaaagaag
    10021 cggcggcatt gaaacccaaa gcctgaaccg catcgtaaaa atgccgtctg aagccctttc
    10081 cagtctcaga cggcattttg ccgcttggcg gcgcagtcgg catacacggc agcaccactc
    10141 cgacatactg cccctaaaat gcccatccgc cttccgcata ataaaaataa cgttcagttc
    10201 attcgacagc agccggacag cccatactac gcggctgaaa aaatgccgtc tgaaacgcat
    10261 tcagacggca tccgcttaaa aaaacaactg attcaaggcc gattaatccg cttccaaaac
    10321 cactttcatc gcttgatttt cggcggcgtg tttgaacacg tcgtaggctt tttccaattc
    10381 gctgaatttg aaacggtggg tcaacatttt ggtgtaatcg acggagctgc tggaaattgc
    10441 cttcatcagc atttcggtgg tattggcgtt taccaggccg gtagtgatgg caagcttttt
    10501 aatccacagt ttttccagtt tgaaatcaac ggattgaccg tgtacgccga caacggcgat
    10561 atggccgccg ggtttcacaa tgtcttggca catattccaa gttgcaggga taccgacggc
    10621 ttcgatcgcg cagtccacgc cgtcttcgcc gacgatagcg acaacttgct tgcttacatc
    10681 gccggaagca gggttaatgg tatgggttgc gcccaactct ttcgccagtt tcaaacggtt
    10741 ttcgtccata tcgcaaacaa tgatggcggc ggggctgtac agttgggcgg tcggcaaggc
    10801 ggacataccg acaggagcgg cacctgcgat gaatacggtg tcgccgggtt tgacatcgcc
    10861 gtattgcacg ccgatttcgt gtgcggtcgg caaagcgtcg ctcaacaaca gggcgacttc
    10921 ttcgttgacg ttgtcgggca gcggcacgag gctgttgtcg gcataaggcg tgcggacgta
    10981 ttctgcctgc gtgccgtcaa tcatgtagcc caaaatccag ccgccgttgc ggcaatgcga
    11041 ataaagttgg attttgcagt tgtcgcaagt acagcatttg ctgacgcatg aaataatgac
    11101 tttatcgccg actttgatgt tttttacagc ctcgccgact tcttctacaa taccgatgct
    11161 ttcatgaccg agaatacgac cgtcggcgac ttcgggtttt tttgcctttc caaataccca
    11221 aatcggtacc gcaaatcgtg gttttgacga ttttcaccac cgcatcggtc ggatcgataa
    11281 tctgcggacg gggtttttct tcaaaacgga tgtcgtttgc gccgtgataa accattgctt
    11341 tcatgctgat actccttgct tgttgataaa taatttcaat accgcaataa agtttcttta
    11401 tatgagttat atgcccctac aaaaaataag tcaataagaa ttattttcac aatgttatac
    11461 aataacatac tgttttaaat ataaataaaa ccaccgattg atattaatga acacacccat
    11521 ccccttctcc gaacggctca tccgctggca aaaacaacac ggtcgccacc acctcccttg
    11581 gcaggtcaaa aacccttatt gcgtctggct ttccgaaatc atgctccagc aaacgcaggt
    11641 cgccaccgtg ttggactact atccgcgttt cttagaaaaa ttcccgaccg ttcagacgct
    11701 tgccgccgcg ccgcaagacg aagtgttgtc gttgtgggcg ggcttgggct attacagccg
    11761 cgcgcgcaac ctgcacaaag ccgcgcaaca agtcgtcagg caattcggcg gcacgtttcc
    11821 gtcggagcgc aaagacttgg aaaccctctg cggcgtaggc agaagcaccg ccgccgccat
    11881 ttgcgccttc tccttcaacc gccgcgaaac cattttggac ggcaacgtca aacgcgtact
    11941 ctgccgcgtg ttcgcccgcg acggcaatcc gcaggacaaa aaatttgaaa actcgctctg
    12001 gacacttgcc gaaagcctgc tgccgtctga aaacgccgat atgcctgcct atacacaagg
    12061 tttgatggat ttgggcgcga ccgtgtgcaa acggacgaaa cccttgtgcc accaatgccc
    12121 gatggcggac atctgcgaag cgaaaaagca aaaccgcacc tccgagctgc cgcgcaaaaa
    12181 aaccgccgcc gaagtaccga ccctgccgct ttactggctg attatccgca accggaacgg
    12241 cgcgattttg ctggaaaaac gccccgccaa aggcatttgg ggcgggctgt attgcgtgcc
    12301 gtgttttgaa agtttgaacg ggctttccga ctttgccgcc aaattctccc tgaccatggc
    12361 agatatggac gaacaaaccg ccctgaccca ccggctgacg caccggttgc tgatgattac
    12421 gccgtttgaa ggacaaatgc cgtctgaaag cccttcagac ggcatttgga taaagccgac
    12481 gcatttgaaa gattacggcc tgcccaagcc tttggaaatt tatttaaacg gtaataggtt
    12541 agaataaaca aaataaaccc attgaactgt tgtttgcagg tatcgcagca agaacaaccg
    12601 atgaatttgg gtcgtatttt aggcggcggg ataatgttca aatgggacat ttggaacgga
    12661 agaagtcggc aatttaaaaa ggatttaaaa agcaaagaag gtcaaaaaca tgaacacaaa
    12721 cttaaatgac aaagacaaag ccatggatac cgcaatcagg tttcagaaaa ggatgaggat
    12781 tccgaaattt ttctttttaa ttctctgaat cacaatggtt ttggcattta tccaagacgt
    12841 gataacgggt tctaattttc tgcaaataac aattaatgta aaattttcgt aaaaatttat
    12901 cggcttttaa aacaaaattg actaaaatag tcgcgagttt ttactgcaat aaaggagatt
    12961 gcaatgaata tgaaaacctt attagcacta gcggttagtg cagtatgttc agttggtgtt
    13021 gcgcaagcac acgagcataa tacgatacct aaaggtgctt ctattgaagt gaaagtgcaa
    13081 caacttgatc cagtaaacgg taacaaagat gtgggtacag tgactattac tgaatctaac
    13141 tatggtcttg tgtttacccc tgatttacaa ggattaagcg aaggcttaca tggtttccac
    13201 atccatgaaa acccaagctg tgagccaaaa gaaaaagaag gtaaattgac agctggttta
    13261 ggcgcaggcg gtcactggga tcctaaaggt gcaaaacaac atggttaccc atggcaagat
    13321 gatgcacact taggtgattt acctgcatta actgtattgc atgatggcac agcaacaaat
    13381 cctgttttag caccacgtct taaacattta gatgatgttc gcggtcactc tattatgatc
    13441 cacacgggtg gtgataatca ctccgatcat ccagctccac ttggcggtgg cggcccacgt
    13501 atggcatgtg gcgtgattaa ataattcgat tgttcgaaac gaaaagtgcg gtgaattttg
    13561 accgcacttt tttgctagat atttagcatt gagacctttg caataacata ggttactaaa
    13621 attttatgct taatctcatt ttcaaaatgc aaaacttttc tgatttttcc tactttttgc
    13681 tcaatattag gaaagtttta ggcaattgaa aattttttgg cgcattttta tgcgtcaaat
    13741 ttcgttaaca gactattttt gcaaaggtc
//
LOCUS       DAXIEB010000051        13756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000051 DAXIEB010000000
VERSION     DAXIEB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.051"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            83..1480
                     /gene="aspA"
                     /locus_tag="ACFOA3_001178"
     CDS             83..1480
                     /gene="aspA"
                     /locus_tag="ACFOA3_001178"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6898187.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1552..2427)
                     /locus_tag="ACFOA3_001179"
     CDS             complement(1552..2427)
                     /locus_tag="ACFOA3_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6898188.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2429..3151)
                     /locus_tag="ACFOA3_001180"
     CDS             complement(2429..3151)
                     /locus_tag="ACFOA3_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6898189.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3174..3653)
                     /gene="ybaK"
                     /locus_tag="ACFOA3_001181"
     CDS             complement(3174..3653)
                     /gene="ybaK"
                     /locus_tag="ACFOA3_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6898190.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3728..4090
                     /locus_tag="ACFOA3_001182"
     CDS             3728..4090
                     /locus_tag="ACFOA3_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6898191.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4125..4988
                     /locus_tag="ACFOA3_001183"
     CDS             4125..4988
                     /locus_tag="ACFOA3_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6898192.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5086..6045
                     /gene="ttcA"
                     /locus_tag="ACFOA3_001184"
     CDS             5086..6045
                     /gene="ttcA"
                     /locus_tag="ACFOA3_001184"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6898193.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6179..6733)
                     /locus_tag="ACFOA3_001185"
     CDS             complement(6179..6733)
                     /locus_tag="ACFOA3_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6898194.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIDGFVIQGGGFTEDLVQKASDKAVANESGNGLKN
                     TAGTVAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRLESGMNTVSKIARV
                     KTATRGFYQNIPVQPVKIRRVVAE"
     gene            complement(6798..7715)
                     /locus_tag="ACFOA3_001186"
     CDS             complement(6798..7715)
                     /locus_tag="ACFOA3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6898195.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     TEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(7776..7901)
                     /locus_tag="ACFOA3_001187"
     CDS             complement(7776..7901)
                     /locus_tag="ACFOA3_001187"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898196.1"
                     /translation="MNLSVVWETVLNVIPAMSADISNLLEQLSIDEEHDNSCHHF"
     gene            complement(7898..8170)
                     /locus_tag="ACFOA3_001188"
     CDS             complement(7898..8170)
                     /locus_tag="ACFOA3_001188"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HFC6898197.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            complement(8167..8460)
                     /locus_tag="ACFOA3_001189"
     CDS             complement(8167..8460)
                     /locus_tag="ACFOA3_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFC6898198.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            complement(8505..8981)
                     /locus_tag="ACFOA3_001190"
     CDS             complement(8505..8981)
                     /locus_tag="ACFOA3_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6898199.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            complement(9052..9507)
                     /locus_tag="ACFOA3_001191"
     CDS             complement(9052..9507)
                     /locus_tag="ACFOA3_001191"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6898200.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(9632..10747)
                     /locus_tag="ACFOA3_001192"
     CDS             complement(9632..10747)
                     /locus_tag="ACFOA3_001192"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6898201.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(10861..11958)
                     /locus_tag="ACFOA3_001193"
     CDS             complement(10861..11958)
                     /locus_tag="ACFOA3_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6898202.1"
                     /translation="MNQTFTLPDTRPYPQNQIKNHLLLNAYQLAHNSSQASRKLSFGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLKNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(12040..13623)
                     /locus_tag="ACFOA3_001194"
     CDS             complement(12040..13623)
                     /locus_tag="ACFOA3_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6898203.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDADLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETIDFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLIFHPPAEV
                     YVEEILADLADRHFLVSAD"
BASE COUNT         3371 a         3759 c         3288 g         3338 t
ORIGIN      
        1 ggtatagtgt tcatcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
       61 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      121 cgagattccc gccgaagtgt attggggcat ccacaccctg cgcgccattg aaaactttaa
      181 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      241 aaaggcgacg gcacaggcaa acggtgagtt gggcgcggtc aagcccgaaa tcgccgccgc
      301 cattgaaaaa gcctgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccgtc
      361 cgacgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      421 caacctcgca ttggaagtct tgggatacga aaaaggccgc tacgacatcg tcaatccgat
      481 ggatcacgtc aatgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      541 cgtgtattac agcatcggcg aattgctcga caaactgact gtcctgaaaa acgcctttgc
      601 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      661 cgtgccgatg actgccggcc aagaattcca atctttccaa gtgttgttgg aagaagaaat
      721 cctcaacctc gaccgcaccc gccaactgct gttggaagtc aatttgggcg caacggcaat
      781 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      841 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      901 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
      961 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1021 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1081 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1141 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1201 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1261 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tgacttatct
     1321 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1381 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1441 catcctctct cccgagaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1501 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1561 gaacagtacc ttgacttccc cccaataaaa caggcaggcg atagaacaca gtgccaacac
     1621 gattcccgcc gtattgattg tgttgatttt ttccttaaag aacaatgcgc ccgaaagcgt
     1681 acccaaaaca atcacgccga tattcatacc ggcaaaaacc aaggtcggat tatccttcat
     1741 catttggtgc gcggtgatgt aggttacgat attcataaaa ttcaaaccgc ccaagaatat
     1801 gccgccgacc acactctcaa cacgccatct gaccgatttg gcaaacaggc aggcaaacat
     1861 cagcacaccc gccagcacaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1921 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     1981 tgccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2041 gaacagtgcg gcaaatgcga ggcacagccc gattagtttg ccttcgctga gtttttcgcc
     2101 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2161 tttgacgata ccggcggctt ccacagattt gcccattatc acgaatacgg acggcagcag
     2221 cacgcccaaa gcggcaaaca gcggccacgt cggcaaaaat gcgccgatat tgccgatatc
     2281 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2341 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2401 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2461 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2521 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2581 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2641 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2701 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2761 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2821 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2881 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     2941 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3001 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3061 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3121 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3181 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc cgggacggat gccgataatg
     3241 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3301 taaatatcca actttgtccg gatgccgaac ggcgttgtgc cgccgaccag atagcccgtc
     3361 cacttgtttg cctgtacggg cgtggcgggt tcgatgtgtt tcgcacccaa atgccgcgcc
     3421 agattgcggg ttgaaatctg cttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3481 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa tagtcgggca
     3541 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3601 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3661 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3721 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3781 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3841 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3901 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     3961 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagcgcg
     4021 gctcgcccgt ccggagtggc gcgtcgaaat caaaataacc gccgtgaaga aagatgcggc
     4081 aaccgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4141 cctttttcgg cgtggtacgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4201 tttccaacga catcaataat cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4261 ccaaaggccg tgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4321 ttatggcgca agacttgctt gaagcaacgg tcaaacgttt gcggatgttt gtgttgcgtg
     4381 ccaaagccgt ttttgaaatc cttgaagatt acgccgtcgg cgcggaattg gaagcatccg
     4441 ccgaaccgct tgccgcgcaa gaacccaacc ttgcctttgc cgcacaacaa gattcagacg
     4501 gcatctgctc tatcgctctg cctcatggcg gcatccttcg catcgccccc aaaaacacct
     4561 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgcagcggtt
     4621 acccgtggat atgcgcggct accaaagaaa ccgccgtcgc ccaaatgctc aaccagcaca
     4681 tcatcggcgg cgtacacttc aaaaaaggct gctatccggg acaggaaatc attgcccgtg
     4741 cccagtatcg ggggcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4801 aagcaggaac cctgctgacg gcagacggcg aagagaccgg catcgtactc gacagcgttc
     4861 aagattcgga aaactttacc gcccttgccg tgattaaatt ctccgccgcg caaaaagaac
     4921 tgaccgcccc aaacggcagt atcttcaaag cggttcatcc cttctttaaa accgaaaatg
     4981 cagaataatc cctctaatgc cgtctgaacc tttttcagac ggcattttaa aaccgcccct
     5041 gttttcggtt acaatgccgc atccccaatt tttcaaacca ccgccatgtc caaaaaaacc
     5101 aaacaagaac tggaaaacaa caaactcagc aaacgcctgc gccacgccgt cggcgacgcg
     5161 attaacgatt tcaacatgat cgagccgggc gacaaaatca tggtctgcct ctccggcgga
     5221 aaagacagct atgccctctt ggacatcctg cgccagctcc aagccagcgc gccgattgat
     5281 ttccaactgg ttgccgtcaa tctcgaccaa aagcagccgg gcttccccga agaagtatta
     5341 ccgacctatc tcgaaagcat cggcgttccc tacaaaattg ttgaagaaga cacctactcc
     5401 accgtcaaac gcgtattaga cgaaggcaaa acgacttgtt cgctgtgcag ccgcctgcgc
     5461 cgcggcatcc tctaccgcac tgcaaaagaa ttgggctgca ccaaaatcgc cttgggacac
     5521 caccgcgacg acatcctcgc caccttgttt ttaaatatgt tttacggcgg caaactcaaa
     5581 gccatgccgc ccaagctggt gagcgacaac ggcgaacaca tcgtcatccg tccgctggcg
     5641 tatgtaaaag aaaaagactt aatcaaatac gccgaattaa aacaattccc catcatcccg
     5701 tgcaacctct gcggctcgca acccaacctg caacgccaag tcatcggcga tatgctgcgc
     5761 gattgggaca aacgcttccc cggccgcatc gaatcgatgt tctccgccct gcaaaacgtc
     5821 gtcccttccc accttgccga taccgaactc ttcgactttg tcggattgga acgcggtcaa
     5881 accctgaaac acggcggcga tttggcgttt gacagcgaaa aaatgcccga acgtttttca
     5941 gacggcagcg aagaagacga aagcgaaatc aaaatcgcgc cgcaaaaagc cgaacgcaaa
     6001 gtcatcaaca ttctggcgaa caagccgaaa acctgcggcg cgtaaccccc cgccgtcaaa
     6061 aatgccgtct gaagcccgtt tggcgtttca gacggtattt taaaatcccg aagtagacac
     6121 ttttcaaccc caaacgcctg ctttttccgt ttcagctttc tgaaggtttc ccgttatatt
     6181 actcggcaac aacgcggcga attttgacgg gctgtacggg tatgttttga taaaagccgc
     6241 gcgtggcggt tttgacgcgg gcgattttgg aaacggtgtt catgccgctt tcgagcctgc
     6301 cgaaaacggt atagccgtat tgtccgtttt tgtagttgag tgaatcattg tccgccagat
     6361 tgataaagaa ttggctggtg gcggaatccg gatcggccgt ccgcgccatg gcgacggtac
     6421 cggcggtgtt tttcaagccg ttgccggatt cgttggcaac ggccttatcg cttgcctttt
     6481 gtaccaagtc ttcggtaaat ccgccaccct ggataacgaa gccgtcgata acgcggtgga
     6541 aaatcgtatt gtcgtaaaag ccttttcggg catagcgcac gaaattagca acggttttgg
     6601 gggctttgga ctcgtccaaa accaaacgga tattgcccat atcggtttcc atcaaaacat
     6661 gggttgccgc catagacggc agggaaaccg ccaaaagcag cgcggttaaa acggttttga
     6721 atttgggttt catcccgtcc tcctaaagcc ttcagacggc attttcattt catatgccgt
     6781 ctgaaagcgc gtgaacgcta ttccaatgcg tctttgagct tttgttcgat taaatccgca
     6841 tcaaacgatt tggcaatcaa ttcaaaacgc gaatcgcgcc gccatgagac ttggttcgcg
     6901 ccccactgtc cgtctaccca gttgagccac acccacgttc ccaatacttg aaacacgcct
     6961 ttggcgcgga cgagtccttc ggtcattttg ggcaggtcgt tgaaaaagtt ggtcagcttt
     7021 tcgccgtcga aatcgcgtcc ggcggggaat gtgaaacctt gcgactggaa gcccatggtg
     7081 ttgtccggca gggctttgag gcggtagcgt gatttttcga tgacgggaat atcgagccac
     7141 cggatgtcga gttgtgcgtt ttggacttcg accactttgg ctttgggcgg gaacagtttt
     7201 gccgctttgt cgtgaaattc ggcaagctgt tcggtggtgc ataaatcggt tttgctggcg
     7261 accaatacgt cgcagatgcc gatttggtct ttatacaacg cctgttgcgc gtaatcgggg
     7321 ttgatgaact ggcgcggatc gacaacggta aagacggcac cgatttccaa aaggctgtcc
     7381 agcggtttgg ctttgagttc gtcgatgacg ctggctgcgt gcgccagtcc gcttgcctca
     7441 atcatcaggc ggtcgggctt ggcatcgcgc agcattttct gcacggttac gcccatttgc
     7501 gggccggcgg tgcaacacaa acagccgccg gcgatttctg ccacagggat gccgttgtcg
     7561 ctcaataccg cgccgtcaat gccgatttcg ccgaactcgt tgacgataat gacccatttt
     7621 tcgttcgggt ctttttgcgc catcaggctt ttgagcgcag tggttttgcc tgttcccaaa
     7681 aaacctgaaa tcaggtggac tttggttttt ttcatttcta tgtgatgtcc cactttaaaa
     7741 tttgaagata gggtgtttta aatgattaaa taatgttaaa agtgatggca gctgttatca
     7801 tgttcttcat caattgacaa ttgttccagc aaattcgata tatcggctga catcgccggt
     7861 atgacgttca aaaccgtctc ccaaacgaca gacaggttca tttcaaagta gccatgggca
     7921 atcctattgc gcaatacccc ctcatcccta tccaattcaa atatttggtt tcctcggcaa
     7981 attccggata cgatttaagg atatgggttg cgttttcacc gataaggaac aaattaaaaa
     8041 cgactgcctg cacagtcttt gtgtcggcag aaaattgccc ataatccatt ttgtcggtat
     8101 atagccggat atattgcgct gcctgcaata tctgtttcaa atagaccgac aattctttct
     8161 gcattttcat aatgcgacag cctcagtcaa caccttatct ctaaagtggg ccgagatgtc
     8221 atcgggtgtc agcaaatcga cctttatgcc caagagtttc tgcaattcct cttgcagccc
     8281 gcccaaatcc aataatgttg tccctgtttt tgcatccacc aacaaatcaa tgtcgctgtt
     8341 ttccgtatca tctccgcgag aaaccgaacc gaataccctt ggattgcaaa tcaatggata
     8401 tttcccgaaa actgccaata tttctttctt tctgctttgc aacaaaagag acggtttcat
     8461 tatctgctcc tttcgaaagg cttattatca atgcaaacca ccgattactg ctgacatttt
     8521 ttacaaatcc cggttaaaac aacgtgttct tctttcagcg caaagccgct ttcggcaacg
     8581 cctgcgcgca gtgccgccca ctcgtggctg agggtttgct cgtccgccgt gccgcattcg
     8641 gtgcagacca aaataaacgc gctgtggtgc gcttcggctt cttcgtggtc gtggcaatgg
     8701 tcgtcgcact cgtgctgcgc gtggctgcac aaaatatagc cgttgaccgc cgccactttg
     8761 tgcaaaacgc cctgctccgc ccaaaaatca agggcgcggt aggcggtagg cggtgcaagc
     8821 acgccctcgc tttgctgctg catctgcgac aagacgttgt aggctttaat cacgccgctt
     8881 tgctgcaaga caatatctaa tacctgctcg cgcaaagcgg ttacctgcaa gccttcgctg
     8941 cgtgcctgtt cgataatttt ctgtttgaaa tttgttttca taaattctct gtttatgccg
     9001 tctgaacaac cgatacggca ggaggcggtt ttatatttgt attcaattgc tttatttgga
     9061 aatcttttcc aacaatgccc gacagcccgc atccgcaagc ctgaaggttt cttcaaaatc
     9121 gcccgtatac cacggatcgg ggacatggtc gtaaccgctt tcgggtatca ggtcggtcag
     9181 cttgaatatt ttttccggcc gcctgccgaa ggttctttcc aattcggaca aattcttgcc
     9241 gtccatcgcg atgatgcagt caaacgccgc cgcatcgctt tggcggattt tgcggctggt
     9301 aaagcctgaa gcatcgatac cgtatttttt caatatcttt gccgtctcgc ggtgcatatc
     9361 ttcgccgtcg tgccagcccg atgtccccgc gctgtccgct tcaaggggaa tgcccgcttc
     9421 ggcggcgcgg cggcgcaaaa tgtattccgc catcggcgaa cggcagatgt tgccgaggca
     9481 gacaaaaagg attttcggtt ttttcatatc ccctccctgt tccggcgcga tgccgtctga
     9541 agcggaaacc ctttcagacg gcatcggtcg gttaatcgta accgcataag gcagaaccag
     9601 ccaaaacatc catctggaag aaatggtttg gctaaatctt aggcatattt aataagtgtc
     9661 caatattaga agccgtatgc tccaaataga gactggcatt tttcaaacta tcttctaaag
     9721 gttcactttt ctccaaaata gaaaaggcag cttggatatt ttcaaatggc agggaaggca
     9781 aatcttcaac gagactgcca caaatagcga caacaggaac tccgacaggg gttctttttg
     9841 ctacaccaat aggcgctttc cctgctaaac tttgacgatc tagtcttcct tcaccaacga
     9901 taaccaagtc aacatctgac actttcttat caaagtcaat caagtccagg caggtgtcaa
     9961 ttccagatac gatacttgcc tgagcaaagg cgcacaaacc accagcgatg cctccaccag
    10021 ctcctgctcc tttaagtttt aatgttgcag gggagacttt ttcataaaaa tcttgtattg
    10081 cctgatctac ggcctcaaac atagtagaat ccaacccttt ttgcttgcca aacgtatagg
    10141 tcgcaccttg gtgtccacat aagggactca caacatctgc taaaatacga atgtgaacat
    10201 cttcaggaat ttcatagcga ttttctgttg aaacagaagc taggtttagt aaggattgac
    10261 cgcaaacggg taaggtattt ccatcctcat cataaaattg ataacctaaa ccagcagcaa
    10321 tcccaatacc tccatcatta ctggccgtac cgccaacgcc aatatagatt tctttaattt
    10381 cttgactaat gaggtggcga atcaattctc caataccacg agtttggatt tgtaatggat
    10441 ttcgtttctc tagcgggatt tttccaagac caaccaaatc agcaacttcg aatagggcta
    10501 gttgttcttt ttgaaaatag cgcatgactt ctttttgtcc aaaaggtcct gtcacttgaa
    10561 gacatttttc ttctaggtca agagaatgtc ggatagcatc tacagtgcct tctcccccat
    10621 caccgacagg acagaggaga cattccacat ctgctatcga ttgttggaag cctcttttta
    10681 ttgcttcagc tacctgttga gctgtcaagc tttccttaaa cgaatccggt gcaattacaa
    10741 tcttcatatt tataattcat cctttcgttt cactcaaggc acaacacaga atgaaaaagt
    10801 gttgtgctct ttattttgat ttattatata aatgagaaag cctatcacta ctacaaatca
    10861 ctatgcgctg aaaaacggat tgtgcccttc ccgtttcaat gcttccgcat agctcgggat
    10921 gctttcctgt tcgcccaagg gattgtgcaa caggtaaacg tgttccacgc ggcactccgc
    10981 catcaggtcg aggaaatttt gctggatgac ggcgatgttg tcggtaaagg aaagctgcca
    11041 cgaaaacacc tgcggcacga cttcaaacgc gccgtcggtc gcattaaacc cgaataaaac
    11101 ctgtccgccc gcctttgccg cagcgaccac gccgacgcgc ggactctgca tacggacggc
    11161 gcgtatcgcg tttgccgcaa acacatccat cgccatacgc tggcgcgtgt ggctgccgtc
    11221 tgaaagcgcg cgtaccggag aatccgggga aagtggattg gcaaacagcg taacgccttc
    11281 cgatgcaaga ttttcctgcc atacctgcat taagggcagg cctgcctgaa gaaggttctg
    11341 acccaatgcc gtctgaacct tgccgctgcc gaaacccagc gcgtaaacga cgtggacgga
    11401 ctgcccttcg ggcaacagca aagggcgcgg ggcgaaacgg cgcaagtgtt gcgccgccgc
    11461 ttcggtattt tgtttggcac gccaccactc atccggcgcg accgcgctca aatcggaaga
    11521 atgcacaaga taaggcagcc attgcgtctc ttgcgtcaac gcgcgcaggt gcggatagtt
    11581 tttcaaacag gcaaacaatg cctccgtcgg cagcttcatc tcgattgccc gttctttctt
    11641 gcagccggac accagcacaa ccggcagggc gaaccactgc acttcgcctt ctttctcgca
    11701 atcgagcacc gcgttcacac tggaaagcag cgcggcataa gttccggcat cgggcgacat
    11761 cgtcaacgcg agggaaaggt tgatatagtg gttttgctca agcatccccc tgatttcggt
    11821 ttgaagctgc ccgaacgaga gtttgcgtga agcctgggaa gaattgtgcg ccaactggta
    11881 ggcattgagc agcaggtggt ttttaatctg attttgggga tacgggcgcg tatcgggcaa
    11941 ggtaaatgtc tggttcatat gtgtcgtgta ccgtttttcg cagtgtgtgt ctttcctgtt
    12001 tgaaaatata tcggacggat tgccgccggg acttgcccgt caatccgccg aaacgagaaa
    12061 atgcctgtct gccaagtctg ccaatatttc ttccacatac acttcggcag gcggatggaa
    12121 tatcaaaccg tcgggcgtgg tgctgacgat gttcaagttt tcggcaaccg tttcaatcaa
    12181 tgcctgtttg ctcgtcggcc gcgacaattg ccgcataatg tacaaatgcc cgtatccgaa
    12241 tgagggggtg ctttcgttgt aacggttggc aggccgaaca aacgcttccg cgccgtgttc
    12301 cacaaaggcg gcggcataat ctgtaaaacg cgccggcaca taggttttat ggtcttcaaa
    12361 aaatggtttg cccgcattgt ctgatgaaac agaaaaacgc ccgagaatgg tttgcaataa
    12421 aagctgggaa ttgattttca tacggtcaaa ttccagaccg ggaaatcggc gggcgaggtt
    12481 ttccgcaaca tcttccgttt taaacggctc tccggttttc atcacatcgc gtatgcccga
    12541 aagcagggta tcgttttcat caaagtcgat cgtttcccct cttgccgggc ggaaattcaa
    12601 actttctatc acttcgacgg caaccgactc atcacgcctg acagtatccc cgacttcctc
    12661 acggcataaa agcgaacggc ggaattggcg gtcggataaa atatcgctgt aaaattcttt
    12721 ggcaatataa tcgccccctg ccaatgccag aatccgctcc cgcgtatgct ccgccatcca
    12781 agaaacaaaa gacgcgtgca aatcggcatc cccgatatac gccagccggt ggcggtttgc
    12841 ccattcgatg aagccgttga cgtaaatcgg gtcgttaaac gcctccatat attcgtgtgc
    12901 gatgtaataa aaattatgat tcaatatttt ttgaatcgcc ggaagtttgc cgccgccgtc
    12961 caagcccttg tcgttttcta aaatttccgc cagcgccttg agcgcgtcca agcctttccg
    13021 cgtccgtgct tccaagggtt cttcaagcac atccctgccg gcaaagtaca taatttcgcg
    13081 caactgctcc tgccgtttcc agccggggta aacattgtat gaaatatagg caatgccgtg
    13141 ttcggtcaag ttgttccggc aaatcgaaaa aattttgtct ttaactgcgt caggcaccca
    13201 cgaccaaatg ccgtggacga tgatatagtc aaacttcccg aatgactcat cgacggtcaa
    13261 aatatctttt tcttccagac gcacattttt caagcccatt ttttcaatga tggcgttgcc
    13321 ctgcgcaacc tgcctgccgg acaagtcgat accgacaaat tccgcatccg ggtaataaag
    13381 agcctgcgtg atgatgttgc cgcccatcga acagcccagc tccaagacct tggcattggc
    13441 ggcgggcgcg ggctgcaaac ccatcatgcg ggcgcgagcc tccaaggcgt tgatggcggt
    13501 ttgggaaaat gctttggatt catacatcaa atcatcatat gaatttttga tgttggacac
    13561 gtccggcaca ccgttctccg ttgcagcacg cgcaaaggcg gcttttttct ttttgttcgg
    13621 catattttgt ttgtctgaag gcactattgc ccgcaagttt aaccaattca tcctacccgt
    13681 tcaactaaat caaatgccat ctgaaggcgc ggagcgtact tcagacggca tctgggaggc
    13741 gcgaaggctt cagacg
//
LOCUS       DAXIEB010000052        13682 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000052 DAXIEB010000000
VERSION     DAXIEB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.052"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..215
                     /locus_tag="ACFOA3_001195"
     CDS             <1..215
                     /locus_tag="ACFOA3_001195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898204.1"
                     /translation="LHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLIP
                     RFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(226..549)
                     /locus_tag="ACFOA3_001196"
     CDS             complement(226..549)
                     /locus_tag="ACFOA3_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6898205.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(628..1284)
                     /locus_tag="ACFOA3_001197"
     CDS             complement(628..1284)
                     /locus_tag="ACFOA3_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6898206.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPDVL
                     KQQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1386..1913)
                     /locus_tag="ACFOA3_001198"
     CDS             complement(1386..1913)
                     /locus_tag="ACFOA3_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6898207.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADETDWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGT
                     VVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAA"
     gene            2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFOA3_001199"
     CDS             2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFOA3_001199"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6898208.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2853..3203
                     /gene="secG"
                     /locus_tag="ACFOA3_001200"
     CDS             2853..3203
                     /gene="secG"
                     /locus_tag="ACFOA3_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6898209.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3217..3302
                     /locus_tag="ACFOA3_001201"
     tRNA            3217..3302
                     /locus_tag="ACFOA3_001201"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3251..3253,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="ACFOA3_001202"
     CDS             4104..4472
                     /locus_tag="ACFOA3_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6898210.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            4469..4780
                     /locus_tag="ACFOA3_001203"
     CDS             4469..4780
                     /locus_tag="ACFOA3_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6898211.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(4749..5003)
                     /locus_tag="ACFOA3_001204"
     CDS             complement(4749..5003)
                     /locus_tag="ACFOA3_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898212.1"
                     /translation="MPRGRGCFGFRARLRRALLSGYAVCTAAVSAWFDAVGHLCGYAL
                     IFGSAVLPVLAGIFSSASIVNLLILPFAKSATQWLRYPCF"
     gene            complement(5161..5895)
                     /locus_tag="ACFOA3_001205"
     CDS             complement(5161..5895)
                     /locus_tag="ACFOA3_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6898213.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHRFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQQILTNTAAQIGTQTILDKKPDTNPELVGLGMDILGTYGLTLPYSRS
                     LEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLK
                     RLLPTVMPVYEHSVRNKGRVNKNRRR"
     gene            complement(6112..6243)
                     /locus_tag="ACFOA3_001206"
     CDS             complement(6112..6243)
                     /locus_tag="ACFOA3_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6898214.1"
                     /translation="MRDFDKPAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            complement(6236..6406)
                     /locus_tag="ACFOA3_001207"
                     /pseudo
     CDS             complement(6236..6406)
                     /locus_tag="ACFOA3_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(6373..7164)
                     /locus_tag="ACFOA3_001208"
     CDS             complement(6373..7164)
                     /locus_tag="ACFOA3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6898215.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTLKAF"
     gene            complement(7393..10023)
                     /gene="leuS"
                     /locus_tag="ACFOA3_001209"
     CDS             complement(7393..10023)
                     /gene="leuS"
                     /locus_tag="ACFOA3_001209"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6898216.1"
                     /translation="MQEHYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYARFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLLKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFNAIAAKLQSQDAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVGEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10290..10805
                     /locus_tag="ACFOA3_001210"
     CDS             10290..10805
                     /locus_tag="ACFOA3_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6898217.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10812..11255
                     /locus_tag="ACFOA3_001211"
     CDS             10812..11255
                     /locus_tag="ACFOA3_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6898218.1"
                     /translation="MPPRLLLGILCCLPIFAPAHAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11424..13481
                     /gene="ppk1"
                     /locus_tag="ACFOA3_001212"
     CDS             11424..13481
                     /gene="ppk1"
                     /locus_tag="ACFOA3_001212"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6898219.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
BASE COUNT         3353 a         3883 c         3379 g         3067 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcaccaccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaatccgc
      601 tttcagacgg catgacctgt caacatctta aaaatcgaaa ccgccaaacg gatcggcatc
      661 cttatcatcc aaatgcgcca ccaaggtatc gaacagcacc ttctcttcaa acacatcgcc
      721 cttgcgcgta atcaaaagcg cgcgctgaac aggcttgcgg cctacgataa ccgccatgcg
      781 tccgccatct tttaactgtt gtttcaacac atcaggcaca aggtttaccg caccgccgac
      841 ataaaccgca tcaaacggcg cacctgcgga aagttcggtc aacccgttgt tttgcacata
      901 atcgatattg tccaaaccca agccgtccaa caccgctttg gcgcggtttt gctgttcggc
      961 atcgatgtcg tccgacacca cacgaccagc caattttgcc aaaagcgcgg tcgcatagcc
     1021 cgaacccgtg ccgatttcca aaaccgtatc gtttttcgtc agcttcaagc cctgcgccag
     1081 ccgcgccacg actttcggct ccagcatctt atgaccgttg gcaagcggca gcgccatatc
     1141 cgcatacgcc aaaccctgca aatcctcatc gacaaaaagc tcgcgcggaa tctccgccaa
     1201 agcgtccaaa acgtcaaaat ccaatacatc ccacggacgg atttgctgtt cgaccatatt
     1261 gaaccgcgct ttttcaaaat ccatttagtg ctccgtcaaa taattgtttc aacaatggca
     1321 ggcattataa aaccacactc ccgccgcgcc aacttcggca cgcgccgccc gcccctttcc
     1381 atcactcaag ccgcctggcg cgaagcctca aaccgcttct ccaaaatctt cgccagttcg
     1441 cccaaataca aagcatctgt ctcatcaaac tgcgccaaat gttcgctgtc cgcgtccaac
     1501 acgccgatac agcggccgtc tgaaaacagc ggcacgacaa tctccgaacg cgacaaagac
     1561 gaacaggcaa tatggtcggg atgcgcgtcc acatccccga cgaccaccgt cccgcccttc
     1621 gcccaagcct gaccgcacac cccgcgaccg aacggaatcc gcgtacacgc caaaggcccc
     1681 tgaaacggtg ccaaaaccaa ttcgtccgaa cgcgtatcga ccaaataaaa acccacccaa
     1741 aaccaaccga acgcctcctt caaaaccgcc gccgtgttcg ccaaattcgc cacccaatcc
     1801 gtctcgtccg ccaccacaga ctcaatctgc ggcaacacct cccgataaag cgcggccttg
     1861 tccgaagccg aaaaatgaag cgcgtgcatc acatctccta tagttgcata catatcaggc
     1921 ggccattata aaacaacctg cccgaaacaa cattccaaac cgcccccgca gccgcccgcc
     1981 ccaagttgcg aacccgccgc atatccacta aacttcacat tgcaacgcgc cacacgcggc
     2041 agacagaaaa aacacgacac ggagcaaaaa agatgtatca ccaaatcgga atgtgggatc
     2101 aaaaatgggt catcggcaac tggaaaatga acggccggct ccagaacaac aattcgctca
     2161 tgcaccgctt ccgcatcctc cccaccgccg aacgcgtcct catcggactc gccgccccga
     2221 ccgtttacct gctgcaactg cacaacgcca tgcaaatcgt cttaaacaac cgcatcctca
     2281 cctgcgccca agacgtgagc cgcttccccg acaacggcgc gtacaccggc gaagtgtccg
     2341 ccgaaatgct cgccgacacc ggcacagaca tcgtcctcat cggacactcc gaacgcagcc
     2401 tttatttcgg cgaaaaaaac gaaatccaac gccgcaaaat ggaaaacgtc ctcaacgtcg
     2461 gactcatccc gttattgtgc gtcggcgaaa gcctcgaaga gcgcgaagcc ggcaaagaac
     2521 acgaagtcat cgcccatcag ctttccatcc tgcaagggct ggataccaaa aacatcgccg
     2581 tcgcctacga acccgtctgg gcgatcggca ccggcaaagt cgccaccgtc gaacagattg
     2641 ccgatatgca cgcattcatc tacaaagaaa tcttgtcttt gtgcggaagc gatgttaaaa
     2701 tccgcgtcct ttacggcgga agtgtgaaag cggacaacgc ggccgacatc ttcgcagtac
     2761 cttatgtgga cggcgcactc gtcggcggcg cgtcattgtc gtacgactcc tttaccgcca
     2821 tcatcagtgc cgcacaaaat gcgtagaaaa atatggaagc cttcaaaacc ctaatttgga
     2881 ttgttaatat aatttccgct ttggccgtca tcgtgttagt attgctccaa cacggcaaag
     2941 gcgcggatgc cggcgcgact ttcggatcgg gaagcggcag cgcgcaaggc gtattcggct
     3001 ctgccggcaa cgctaacttc ctcagccgct cgaccgccgt tgcagcaaca tttttctttg
     3061 caacctgcat ggctatggtg tatattcaca cccacacgac aaaacacggt ttggacttca
     3121 gcaacgtaca acaaactcag caagcaccca aacccgtaag caataccgaa ccttctgccc
     3181 ctgttcctca gcagcagaaa taacagtttt tcaaatgccg acatggtgaa attggtagac
     3241 acgctatctt gagggggtag tggccgtagg ctgtgcgagt tcaaatctcg ctgtcggcac
     3301 caacacacaa aacgcttaga aattctaagc gttttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgttcccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt ttttttgggt ttttggaaaa aacctatact atagtggatt
     3661 aaatttaaac cggtacggcg ttgcctcgcc ttgccgtacc ggtttaaatt taattcacta
     3721 taaaataaga aaaagataaa aaattggtaa caaatgcggt aacaaatggt aacgaatcgg
     3781 taacaacttt tggggttttc cggtttttca ccgtcttggc agtgggagcg tagcggaatg
     3841 aaaagccaaa acgcacggaa ccgcgcctat tttgagcagg aatggcggtt aaaccgcttg
     3901 gttatatacg gggcatagga agacagcgaa acgcgctaaa aacgcaattt gagccattaa
     3961 aagcgattga ttaaaaaata atcaggttag ccgccggtgt ttcaggcggc ataaacggag
     4021 aaattgcggg gcataaaaaa ggcagcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaagca caaaatcaac gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaattat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggggaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cgcgctgctg aatatgcctg ccaagacagg caagaccgcc
     4861 gaaccgaata tcagggcata gccgcacaaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcggcggtgc agacggcata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgcgccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaagg caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga ctcccttccc
     5161 ttaacgccga cggtttttat taacgcgccc cttatttctg acactgtgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgtttg tcggatgagt
     5281 agaggtaata gcataaataa agccgttttg gtcgttttcc tgattcattt tttcccaaac
     5341 cctgacagcg gccgccggat gatagcctgc ctgcgccatc aacatcattc ccccctcatc
     5401 ggcttcttct tccaagctgc ggctataagg caaggtaaga ccgtacgtcc ccaaaatatc
     5461 catacccaat ccgaccaatt ccggattagt gtccggtttt ttgtctaata tagtctgcgt
     5521 gcctatctgc gccgccgtat tggtcaaaat ttgttgcccg accttatttt taccgtgttc
     5581 atgcagggcg tgcgtcattt catgccccat aatggcggca atttcgtcat cggtcagctt
     5641 gagtttgtcg actatccccg tataaaacgc catttttcca ccgggcatcg cccacgcgtt
     5701 cagctcatcg tttttgaaaa ccgtcatttt ccagtcaaac ctatggccgg tattatttgc
     5761 cgcatcggca taaggcagca tacgtcgaaa tactgcctgc accctgcggg ctgttctgga
     5821 tgtggtatcg acattgccgg cagacttgtt caactcaacc gttttcatat aatctttggc
     5881 agccgcagcg ttcattgtgg cagaatcatg accgtaaaca tcagcaacga ccgcacaacc
     5941 tcccagtatc gaaatgactg ccgataggca aactatctgt ctaaaggaag gaaggaagga
     6001 aatttcatat ttaggtttac tccttaaaaa attaaatttc aaaaaatgcc gtctgaatcc
     6061 aaaacggatt tcggacggca tcttaacatt gtttaatgtt tttaaaaaga tttacaccac
     6121 gatgttcgcc agtctgcccg gtacggcgat gattttcttg gcaggcttgc cgtctatgaa
     6181 tttcaccgcg ccttcagcgg cgtgttcggc agcttcttca gccggtttat cgaaatcacg
     6241 cataaattgc caataattct ccgactttct tacgactact acctttggcg gcaatattgc
     6301 gctgtacttc aactgttttc agaatggcag aagaataaat atcccttgtg aattcagtat
     6361 catgatttga aatcaaaacg ccttgagagt cggacgcaat ttgtcgattt ttttgtaaag
     6421 tccgcgacca atgaattcga tcgtattttg gtcgcgcaaa atttgcaact gttggcggat
     6481 tttgtctttg atatggttgt tttggggaaa ttggatggat agtttgtttt caaattcata
     6541 catttgcgac aatgtgaatt cttcggggag ttggtcgata catttcataa cagccaaaag
     6601 ccagcctttg cgctccgcat tttggttgcg taaaaacagg ttggattgcc attttttcag
     6661 aacggtttcg ggttcgataa tgcgggaatc gtctattaag aatatttttc cgctttcagg
     6721 caaaggggcg agattgatgg aacacataat gtggtgcggc cggtttttaa tgcctttatt
     6781 tctaggaata atcatatccg gcgtgatgaa atgtttgggt acaagcacca attgccgtat
     6841 ggagtaatcc gcttttttat atgcaagaaa gaaaaagttg gggttggtat ctgaccggat
     6901 gcgctccaac atagtgcgat atgcaccgtc aggcacgctg ttgcctatgg ttttttgatt
     6961 tttactcttt aattcatatt gctcgtggca atttgggcaa aagaggtctg caacaggttt
     7021 gttattggca aatctcttca tcggcttgct tccgcaacag gggcagtagc cgtttttttc
     7081 cagccaagcc tcgctcatta cacggatttt gtgggttgct ttattttgtt gctttcccaa
     7141 ttcagtatcg aaaaataaat tcatgttttg gattttgaga ttaagtttta gtggattaac
     7201 aaaaatcagg acaaggagac gaagccgcag acagcagggc aaggcggggc aacgccgtac
     7261 cggtttaaag ttaatccact atatttgagg tctgtcatgc ggacaaccca atctgcctta
     7321 aaaaggccgt ctgaaaccct gtttccaagt ttcagacggc ctttatccgt atggctaaac
     7381 cttaaaagcg gcttagacga cgatattcac caagcgtccg ggaacgacga tgattttctt
     7441 cgcgggtttg ccttccataa atttcaccgc gccttcggtg gcgagtgcgg cggcttcgag
     7501 gtcggctttg gatgcgtcgg cggcgacggt gattttgccg cgcagtttgc cgttgacttg
     7561 aaccatgact tcgatttcgg atttgaccaa ggcggcttcg tcgactgccg gccagcctgc
     7621 ttcccacagt ttcgcgccgt tcaattcgct ccacagggtt tcgcagatgt gcggcacgat
     7681 gggccacagc aggcgtacgg cggtttccaa tacttcttgg gcgacggcgc ggccttgttc
     7741 gccgccggtg tcggttttgt cgtattggtt gagcaattcc atcacggcgg cgatggcggt
     7801 gttgaactgc tggcggcggc cgtagtcgtc gctgactttg gcgatggtag aatgcagttt
     7861 gtggcgcagg tctttgagtt ctttagacaa accgtcttgg ctgcctgcga acgctttgac
     7921 tgcttcgcct tgcttcaagt attcgtaaac ggtacgccac aggcggcgca ggaagcggtg
     7981 tgcgccttcg acgccgctgt cgctccattc gagggactgt tcgggcggtg cggcgaacat
     8041 catgaacagg cgggcagtgt ccgcgccgta ggcgttaatc agctcttgcg gatcgacgcc
     8101 gttgtttttg gacttggaca ttttttccgt gccgctgatg acgacgggca gaccgtcggc
     8161 tttgaggacg gcggaaacag ggcggccttt gtcgtcgaac gtcagctcga catcggcggg
     8221 gttgatccaa tctttgccgc ctttgtcgtt ttcgcggtag taggtttcgc aaacgaccat
     8281 gccttgcgtc agcaggcgtt caaacggttc gccaacattg accaagcctt cgtcgcgcat
     8341 cagtttggtg aagaaacgcg cgtacaggag gtgcaaaatg gcgtgttcga taccgccgat
     8401 gtattggtcg accgcgcccc agtatttcgc ggcttctgcc gataccatgc cgtctgaaaa
     8461 cttgggcgac atatagcgga agaagtacca gcttgattcc atgaaggtgt ccatggtgtc
     8521 ggtttcgcgt ttcgccgcgc caccgcagca tgggcaggtg gtttcgtaaa actcgggcat
     8581 ttttgccaaa ggcgaaccca tgccgtcggg tacgacgttt tcaggcagga cgacgggcag
     8641 ttggtcggct gggacgggta cgtcgccgca tttttcacaa tggacgatgg gaatcgggca
     8701 gccccagtag cgttgacggg aaatacccca gtcgcgcagg cggtattggg ttttcggttc
     8761 gcccgcgtct tggctttgca gcttggcggc gatagcgttg aaggccgtct gaaaattcat
     8821 gccgtccaag tcgccgctgt tgaccaatac gccgttttct ttgtcgccgt accattcttg
     8881 ccattggttt gcgtcgaatg cgttgtcacc gacggcaatg acttgttttt tcagaagatt
     8941 gtatttggtg gcgaactcga aatcgcgttc gtcgtgcgcc ggaacagcca tcaccgcgcc
     9001 atcgccgtag ccccacaata catagttggc aatccacact tccagcttgt cgccgttgag
     9061 cggattgacg acgtagcggc cggtcggcac gccttttttc tccatcgtcg ccatatcggc
     9121 ttcggcaacc gagccggctt tgcactcggc gataaatgcc tgcaattcgg gtttgtcggc
     9181 ggcggctgcg gttgccagcg gatgctcggc ggcaacggca acataagtcg cgcccatcag
     9241 cgtgtcgggg cgggtggtat aaacttgcag gaatctcgcg taatcgcctt ccaaaccttg
     9301 tttgctgtcg tctgaaacgg caaagcgcac ggtcataccg cgagatttgc cgatccagtt
     9361 gcgctgcatg gttttgactt gttccggcca gtgttccagc ttgtccaaat cgttgagcaa
     9421 ctcttcggcg taatccgtga ttttgaagta atacatcggg atttcgcgtt tttcaatcaa
     9481 cgcacccgaa cgccagccgc gcccgtcgat gacttgctcg ttggccaata cggtttggtc
     9541 gactgggtcc cagtttaccg tgccgttttt gcgatacacg atgccttttt caaacagctt
     9601 ggtaaacagc cattgttccc agcggtagta ttcgggtttg caggtagcga cttcgcgctc
     9661 ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc atgtattcga tgttgtcgta
     9721 agtccaagcg gcgggggcga cgttgttttt catcgccgca ttttccgcag gcataccgaa
     9781 cgcgtcccaa cccataggtt gcatgacgtt gaagccgttt aagcgtttga agcggctcaa
     9841 tacgtcgccg atggtgtagt tgcgcacatg ccccatgtgc agcttgccgc tggggtaggg
     9901 gaacatggag aggcagtagt atttgggttt ggaagcgtct tcggagacgt tgaaaatacg
     9961 ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg gcgggccggt aatgttcttg
    10021 catggtcatt ctgtttttcg cttgaaaacg ttaggaattt aaaggaggaa ttataacagg
    10081 ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc gtaaattcgc acgcgcatca
    10141 ttccgtatgc cgtacaaata caccgcgttt actgatacgc acgtttttta tgctaatatt
    10201 acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc tcttccgaac aaaaggcaga
    10261 cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa acaaatcgtc aaaccgggct
    10321 tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac caaaagcaac gtcaaagccg
    10381 acggaacgac cgacaatccg gttttcccga aaccctattc cgtaacgctc gacaacaatc
    10441 gcggtacatt cccgacctat gacgaattgg acttgatgcg tcccggtctg accaaagacg
    10501 acatctacaa aatcctgggt cgcccgcatt acgacgaagg tatgtacggc gtgcgcgaat
    10561 gggattacct gttccacttc cataccccgg gcgtaggtat cgaccctgaa aacacttccg
    10621 gcgtagaaga tgttactacc tgccaataca aagtgatttt cgataaagac aaatttgccc
    10681 gcagcttcta ctggaacccc gtcttcccga aagatgccgc ctgtccgccg cccgcaccca
    10741 aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc aaaaccgaaa cgtatccgcc
    10801 aataatacgc aatgccgccc cgcctgcttt tagggatatt gtgctgcctg ccgatttttg
    10861 cccccgcgca cgcgcagggg cagccggata cggtcggcga ctttatccaa aagaaaaaag
    10921 tcatcgtcga tacatccaaa gcggaactct gttttgccga tgacaggcag tgccaccccg
    10981 tcctcatcgg tgttgccacg cccaagggga cgttcgggct gacgctgaac agtaccgaca
    11041 agcccggata cggcggcgaa gtcatcggtt tcaagcagga gggtgatttt cttttcgccc
    11101 tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa cgaacgcatc gcctccccgt
    11161 ccgtgtccga caggattatg accaacggct gcatcaacgt cagcgatgcg gtgtacgaaa
    11221 aactgcgtca ttattttgtg ttggaagtga tttgaaacag acggataccg cacgcgccgg
    11281 tatctgtttt cacattgccc cgatgcctga aacagactgt ccgccacgtc atgccgtctg
    11341 aaaccggcgc agatgccgcc aagccttcag acggcattgc ctgcccgctc cgaccgaaca
    11401 acaaccatct ttgggagaac cttatgcccg aacaaaaccg catcctctgc cgcgaactaa
    11461 gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga agacaaaaac gtcccccttt
    11521 tggaacgcct gcgcttcctg tgcatcgttt catccaacct cgacgagttt ttcgaagtcc
    11581 gtatggcgtg gctgaagcgc gaacacaaac gctgcccgca gcgcaggctg gacaacggca
    11641 aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc gcgctccctg atacggcacc
    11701 agtacgacct gttcaacaac gtccttcagc ccgagctggc acaagaaggc atccattttt
    11761 accgccgccg caactggacg ggcacgcaga aaaaatggat cgaagactat ttcgaccgcg
    11821 aactgctgcc gatcctgacc cccatcggac tcgacccgtc ccaccccttc ccgcgaccgc
    11881 tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac ggacgcattc ggcaggcctt
    11941 cggggatggc gattgtgcag gcaccgcgca tcctgccgcg cgtcgttccc ctgccgtccg
    12001 aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc catcctccac gcccacgtcg
    12061 gcaaactctt cccgggcatg aacgtcaaag gctgccacca gttccgcctg acgcgcgaca
    12121 gcgacttgac cgttgacgaa gaagacctgc aaaacctccg cgccgccatc caaaacgaac
    12181 tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt cgccgacacc tgtcccgcct
    12241 acatccgcga ctttctgctc gcgcaattca aactgaccgc cgccgaactc tatcaggtca
    12301 aaggcccggt caacctcgtg cgcctcaacg ccgtccccga cctagtcaac cgccccgatt
    12361 tgaaatttcc cacacacacg ccgggcagac tgaaagcctt gggcaaaacc gcgtccatat
    12421 tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc ctaccaatcg ttcgaccccg
    12481 ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt gcttgccgtc aaaatgacga
    12541 tttaccgcac cggcacgcgt tccgaactcg tccgcgccct gatgaaggcg gcactcgccg
    12601 gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt tgacgaagcc aacaacgtca
    12661 actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt gtacggcgtg ttcggctaca
    12721 aagtccacgc caagatggca ctggtcatcc gccgcgaaga cggcgtactc aagcgttacg
    12781 cccacctcgg cacgggtaac taccaccaag gcacatcgcg catctacacc gacttcggca
    12841 tcattaccgc cgacgaacaa atcaccgccg atgtgaacac attgtttatg gaaatcacag
    12901 gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag cccgtttacc ctgcacaaaa
    12961 tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa agccggcaaa ccggcgcgga
    13021 ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat cgaagccctg tatcgggcaa
    13081 gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat gtgcaccttg cgcccgggtg
    13141 taaaaggctt gtccgaaaac atccgcgtcc gctccatcat cggcaggcag ctcgaacacg
    13201 cgcgcgtgta ttgcttccac aacaacggcg cggacgatac ctttatctcc agcgcggatt
    13261 ggatggggcg caacttcttc cgccgcatcg aaaccgccgc gccgattacc gcgcccgaac
    13321 tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcatt ggcagacaat acgcacgcgt
    13381 ggctgatgca gcccgacggc ggctatatcc gcgccgcacc tgccgagggc gaatccgaag
    13441 ccgacctgca aaacgatttg tggacactgc tcggaggctg acccgcaccg ccccaatcaa
    13501 aaaccatgcc gtctgaaacc tttccgtttc agacggcatg gttttacagc aatctaaaca
    13561 gggcggaccg gagtcaaaaa cacaccttcg ccatttccgc ataagcactt cccctatacg
    13621 ctcccaaccc caatccgccg cattccagac ggcattatag tggattaaat tttaggggct
    13681 gt
//
LOCUS       DAXIEB010000053        13294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000053 DAXIEB010000000
VERSION     DAXIEB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.053"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(305..1201)
                     /gene="argB"
                     /locus_tag="ACFOA3_001213"
     CDS             complement(305..1201)
                     /gene="argB"
                     /locus_tag="ACFOA3_001213"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6898220.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKAGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1371..2501
                     /locus_tag="ACFOA3_001214"
     CDS             1371..2501
                     /locus_tag="ACFOA3_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6898221.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2572..3423
                     /gene="lgt"
                     /locus_tag="ACFOA3_001215"
     CDS             2572..3423
                     /gene="lgt"
                     /locus_tag="ACFOA3_001215"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6898222.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3506..4168)
                     /locus_tag="ACFOA3_001216"
     CDS             complement(3506..4168)
                     /locus_tag="ACFOA3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6898223.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4263..6056)
                     /locus_tag="ACFOA3_001217"
     CDS             complement(4263..6056)
                     /locus_tag="ACFOA3_001217"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6898224.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6301..7410
                     /gene="proB"
                     /locus_tag="ACFOA3_001218"
     CDS             6301..7410
                     /gene="proB"
                     /locus_tag="ACFOA3_001218"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6898225.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7423..8685
                     /locus_tag="ACFOA3_001219"
     CDS             7423..8685
                     /locus_tag="ACFOA3_001219"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6898226.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRNRIEAGLPPVRFRLDPQAARHIGGEAAGADNFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(8910..11942)
                     /locus_tag="ACFOA3_001220"
     CDS             complement(8910..11942)
                     /locus_tag="ACFOA3_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6898227.1"
                     /translation="MFWIVLIVILLLALVGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKMAVYWLFVGVVRFCRNYLAHESEPDRLVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMTAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNV"
     gene            12107..12787
                     /locus_tag="ACFOA3_001221"
     CDS             12107..12787
                     /locus_tag="ACFOA3_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6898228.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLALYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12816..13175
                     /locus_tag="ACFOA3_001222"
     CDS             12816..13175
                     /locus_tag="ACFOA3_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6898229.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT         2915 a         3799 c         3436 g         3144 t
ORIGIN      
        1 aaagaaacct tttttcccga taagtttccg tgccgacagg tctagattcc cgcctgcgcg
       61 ggaatgacga aatttcaaag ttatggcgtt atcggaaaaa caaaaaatca aagccggaga
      121 atttatccca aacaaccgga tttcaaaaaa accagattcc cgccttcgcg ggaatgacgg
      181 attttaggtt tctgttttgg tttctgtttt cgcgggaata acaacttaga aattgcccga
      241 aaccccaaaa acagaaaccg gacaaacggg ttttccgcca aagccggcat tttccgactt
      301 tgcttcaggc atcttcccca ccgcccaaaa tcatcgaacc gataccggca tcggtaaaga
      361 tttccagcaa aagcgcgttg ggcaacctgc cgtcgatgat atgcgtggct ttcacaccgt
      421 tgacggcggc ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt
      481 cggcaatcag ttcatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt
      541 ccatcacacc ggcgatattc gtcatcatca agagtttttc agcgttcaat tcttccgcca
      601 atttgcctgc caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga
      661 cgggggcgac gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga
      721 tgctttccac cgtaccgacc tgtccgatgt ccacgccatt ctgttcgggc gtatcgatca
      781 aaagtttctt cgccttaatg aaatgatcgt cgcgtccgct cacgccgacc gcgtgtccgc
      841 catatgtgtt aatcatcgac acgatttctt tattgacatg cccgcccaac accatttcga
      901 caatatccat cgcctctttg tcggtaacgc gcattccttg gacaaactca ccctttttgc
      961 cgactttttc aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa
     1021 tgccgaccag cttcagcagc acgacatcgc gggcaaaccc tgctttcaag gcaggttcgg
     1081 tcatcgcgtt gccgccgtac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag
     1141 gcagcgcttc ggcaaggata cgcgctttgt cggcggcgga aataatgttt tcagactcca
     1201 taacgactcc ctattgttga caatttcggc ttatgttaac gccaaacgcc gtctgaaaca
     1261 atcttcagac ggcatccggc acaccttcgg gcaggttgaa gccttgcgat acgggcggta
     1321 aaatcgcggt ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa
     1381 tcagcacccg cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgac ccttccaaca
     1441 tccgcctcgc cctgcgtccc gacaacgcct ccgattttgc acaatggttc tacttccggc
     1501 tgcaaggcgc ggcatatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct
     1561 acccgaaagg ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc
     1621 gcgtaccgac ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca
     1681 gcgtgtatta cgcctatttc gaaccttact ccgaagaaca gcatttaaac ctcctcggcg
     1741 acgcgcaagg cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caagggcgcg
     1801 acatcaatct gctgaccatc ggcaatcagg tcgaaagcga tctgaaaatc tggattaccg
     1861 cccgccagca tccgggcgaa accatggcgg aatggtttgt cgaggggctg ctcggcaggc
     1921 tgctcgatcc gcaagacccg acagcgcgca ccctgctcga ccgcgccacg ttctatatcg
     1981 tccccaatat gaaccccgac ggttcggcac tgggcaacct gcgtaccaac gccgccggtg
     2041 cgaacctcaa ccgcgaatgg gaaaatccga ctttggaaaa aagccccgaa gtgttcttcg
     2101 tgcgcgaaaa aatgctggaa accggcgtgg atttgttctt ggacatccac ggcgacgaag
     2161 gactgccctt tgtctttgtt gcaggtacgg aaggcgtgcc gaactacaat ccgcgcattt
     2221 ccgcgttgga aacacagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg
     2281 aatacggtta cgaaaaagat gcgcccggtc aggcaaacat gaccttggca acaaactggg
     2341 tcggcaaccg tttcaactgc cttgcctata cgttggaaat gccgttcaaa gacaatgcca
     2401 acctgcccga cgacgatttc ggatggaacg gtcaacggtc gttgcgcctg ggcgaagccg
     2461 cgttgtccgc catcctcaac gtcatcggcg atttgcgctg aaacccttat tgcagaaatg
     2521 ccgtctgaag catcatgcct ttcagacggc atttgaaaac gaaagcctgt tatgattatc
     2581 catccccaat tcgatcccgt cctcatcagt atcggcccgc ttgccgtccg ctggtatgcc
     2641 ctaagctaca tcctcggatt tattcttttt acctttctcg gcagaaggcg catcgcgcaa
     2701 ggcttgtccg tttttaccaa agaatcgctc gacgacttcc tgacatgggg cattttgggc
     2761 gtaattttgg gcgggcgttt gggttacgtc ctgttttaca agttttccga ctacctcgcc
     2821 catccgcttg atattttcaa ggtatgggaa ggcggaatgt cgttccacgg cggctttttg
     2881 ggtgtagtta ttgccatatg gttgttcggc cgcaaacacg gcatcggctt cctcaaactg
     2941 atggacacgg tcgcaccgct cgttccgctg ggtctcgctt cgggacgtat cggcaacttc
     3001 atcaacggcg aactttgggg acgcgttacc gacatcaacg cattttgggc aatgggcttc
     3061 ccgcaggcgc gttacgaaga tgccgaagcc gccgcgcaca atccgctttg ggcagaatgg
     3121 ctgcaacaat acggtatgct gccgcgtcat ccctcgcagc tttatcagtt tgcacttgaa
     3181 ggcatctgcc tgttcgccgt cgtttggctg ttctctaaaa aacagcggcc gaccggacaa
     3241 gtcgcctcgc tcttcctcgg cggctacggc atattccgct tcattgccga attcgcacgc
     3301 caacccgacg actatctcgg gctgctgacc ttggggctgt cgatggggca atggttgagc
     3361 gtcccgatga ttgttttggg tatcgtcggc tttgtccggt tcggcatgaa aaaacagcac
     3421 tgaccgcctg tgaaaaatac cgtctgaaac atggaaatcc ggtttcagac ggcattttta
     3481 cgtttggttc atggaaacaa acgcttcaat tcttaccgca acacgccttg tattttctgc
     3541 cgctgccgca cggacaggga tcgttcctgc ctgttttttc gccttccctg cggacggttt
     3601 gcggcttgtt gatgaccgcc tgccagtagc ggtagatatc cgccaatgca taaggcaact
     3661 cggattccag ttccgccagc tcgccttctg tgaattgcag acggatagcg ccgttgtcct
     3721 cttcgtcgta aataccgccc aatgccatga tgggataaaa caactcttca aacgcttcat
     3781 catcgacggc ttcaaaccaa tcggtcggca caatatccaa accgtaaaga taagcattgc
     3841 accatgtgta aaaatcgctg ccgccgtctt cgttttcata cagccacaaa tcgggcagtt
     3901 ttttatccga catcgcggcg gttgtttcca tcgccattgc caaaaccagc cgttcgattt
     3961 cggaacgttc ggcggcggta aattgcgatt cgtcgcccaa cacttcgggc agccagtcga
     4021 gcggtgtcaa tttgtccggc ccgctcaaca gcgccgtcat aaaaccttga acctcgtcgc
     4081 aacgcatcgt gttgccttgt tcgcttttgg catccaacaa ttcgctcaac cgccgtttgg
     4141 atgcttcggt aaattttcgg gaatccatca ttttcctttt caaatgggtt ttgcgcccta
     4201 ttatcgccgc aatgccgtct gaagcctttt tatgcttcag acggcatcgt ctgttttcac
     4261 gctcaaatcg taccgctgcc ctgcgctttc ggtttggcgg cactaaattc cagcttgctc
     4321 aaagcggaaa ggtaggcttt ggcggtggcg accaaaacgt cggtatccgc gccctgaccg
     4381 ttgacgacgc ggttgccgcg cgccagacgg acgctggttt cgccctggct ttccgtacct
     4441 tgcgtgacgg cgttgacgga ataaatctgc aaagccgcgc cgctttgcgc cacgctttca
     4501 atcgctttga aaatcgcatc cacggggccg gagcctgtgg cggaagcgcg tttttcttcg
     4561 cctttgatgc tgaacacgat gtcggcgcgc ggctcttcgc cggtttcagt gctgattttt
     4621 tgggagatga atttgtagct ctcggcattc atgctgccca tttcgtcgga taccagtgcg
     4681 tgcaggtctt catcgaagat ttcgcgtttt ttgtcggcga gttctttgaa gcgggcaaac
     4741 gcggcgttca gtgcctcttc gctttccaac tcgatgccca aatccgccag cttggttttg
     4801 aaggcgttgc ggccggacaa tttgcccaag ctcaaacggt ttgttgccca gccgaccgat
     4861 tcggcggaca taatctcgta agtttcgcgg tgtttcagca ccccatcctg atggatgccc
     4921 gattcatgcg aaaaggcatt ggcaccgaca atggctttgt tgggctgcac gggatagccc
     4981 gtaatggtgg acaccagttt ggacgaaggc acgatttgcg tggtatcgat gccggtttcc
     5041 aagccgaaca agtcgtggcg cactttcaac gccatcacga tttcttcaac cgaagcattg
     5101 cctgcacgtt cgcccaagcc gttgacagta cattccacct gacgcgcacc gccttttaat
     5161 gcggcaagcg aattggcaac cgccaagccc aaatcgttgt ggcagtgtgc cgaccaaacg
     5221 actttgccgc cgttgggcgt tttggcaatc agttcgcgga aaaattcttc ggttttgtac
     5281 gggatggaat agccgacggt atcgggaata ttgatggtgg tcgcgcccgc ttcaatcacc
     5341 gcgccgcaga tttcggcaag gaaatcgatt tccgaacgca acgcgtcttc gcaggaaaat
     5401 tccacatcgt cggtgtattc acgagcgatt ttcaccgctt tgaccgccgc ctcaatcacc
     5461 tgcttcggct tcatcttcaa tttgtactcc atatggatgg ggctggtggc gatgaaggtg
     5521 tggatgcgtt ttttcggcgc gggcgcaacg gcctcacccg cctgacggat gtcccgctcg
     5581 atggcgcggg acaatgaaca gaccgttgat ttggtaatgg ttttcgcaat cgcattgacc
     5641 gcctcgaaat cgcccgggct ggcagcggca aaacccgctt cgatgatgtc cacacccaat
     5701 ttttccagct ggcgggcgac gcggattttt tcctctttgg tcatagcggc gccgggcgac
     5761 tgctcgccgt cgcgcagggt ggtgtcgaaa atgataacgc ggttggcctg tgtcatttct
     5821 gttctctcca gagattcgtt tttttgtaaa aaagcattca aatccagcgg cctgccttgc
     5881 gcttccgcca acgcggtcag cttttgcagt tgcgccagag gcagcgagcc gcgcgtgcgc
     5941 catttataga tggcggcggt cgtcgcggca ttttcgggat cgtgctgttt caacgcttcg
     6001 gcaagcgcgt tcacgccgcc gaaataagca accaagcggt ctatatctaa ttgcatggta
     6061 gtccttgtct aaaataaatc agcctacagc agaaatatgg acaggattat acgcattttg
     6121 gacaaaacgg caatataaat ttgaaaaatc tcaataagcc gccatagatt ttattaattt
     6181 tatacatttt gtccatttta ttgcagacgc ggcgtgattg ttgacaattg cggcagaaca
     6241 gggcaaattc ggcggcacaa ggtggcaacg agacaggaca aaccaacaag gacggaaaca
     6301 atgaaataca aaagaatcgt atttaaagtc ggcacatctt cgattaccca ttcggacggc
     6361 agtctctcgc gcggcaaaat ccaaaccatc acctgccagc ttgccgcatt gcatcatgcg
     6421 ggacacgagc tggtcttggt gtcttccggc gcggttgcgg cagggttcgg tgcgctgggt
     6481 ttcaaaaaac gtccggtcaa aatcgccgac aaacaggctt ccgccgccgt cgggcagggg
     6541 ctgctgatgg aagaatatac ggcaaacctg tcttcagacg gcatcgtgtc cgcgcaaatc
     6601 ctgctcagcc gcgccgactt tgccgacaaa cgccgctacc aaaatgccgg cggcgcactt
     6661 tccgtgctgc tgcaacgccg cgccgtcccc atcatcaatg aaaacgatac ggtttcggtt
     6721 gaggaattga aaatcggcga caacgacaca ttgagtgcgc aagtggcggc gatgatacag
     6781 gcagacctct tggtgctgct gaccgacata gacggtcttt acacgggcaa cccgaacagc
     6841 aatcccgatg ccgtacggct ggacaaaatc gaacacatca accatgaaat catcgaaatg
     6901 gcgggcggat cgggttcggc aaacggcacg ggcggtatgc tgaccaaaat caaagcggca
     6961 accatcgccg ccgaatccgg cgtaccggtg tatatctgtt cctcgctcaa acccgatgca
     7021 cttgccgaag ctgccgaaca tcaggcggac ggctcgtttt tcgtcccccg tgccaaaggt
     7081 ttgcggacgc agaagcaatg gctggcgttc tattccgaaa gccggggcag cgtttatgtg
     7141 gacgaaggtg cggaacacgc tttgtccgaa caggggaaaa gcctgctgat gtcgggcatt
     7201 gccggaatcg aagggcattt ttcccgtatg gacaccgtaa ccgtgtacag caaggcaacc
     7261 aaacagcccc tgggcaaagg gcgcgtcctg ttcggttctg ccgccgccga agacctgctc
     7321 aaatcgcgta aggcgaaagg cgtgttcatc catcgggacg actggatttc catcacgccc
     7381 gaaatacgcc tgcttctgac cgaattttag aaaaggaaac ccatgtcaaa cacacaaaaa
     7441 cagcttgccc ttgccaaagc ggcaaaaaaa tccgtcaaca cggcggatac ggaagaaaaa
     7501 aaccgcgcgc tgcttgctat ggcggacagt ctggaagcgg cggcggcaga tattttggcg
     7561 gcaaaccgtc aagacttgga agccgcggca ggcaacattc ccgaaagcat gaccgaccgc
     7621 cttttgttgg acggcaaacg catttgcgcg atggcggacg gcattagggc ggttaccgca
     7681 ctgcccgacc ccgtgggcga aatactggaa acctcgactc tgcccaacgg cttggaaatc
     7741 gtcaaaaagc gtgtggcgat gggcgttatc ggcattattt acgaaagccg cccgaacgtt
     7801 acgtccgatg cggcggcttt ggcactgaaa agcggcagcg cggtcgtact ccgcagcggc
     7861 aaagatgcat tccaatccgc acgcgccatc gttgccgccc tgaaaacggg gttggcacaa
     7921 acccgcatcg accccgacgc gctccagttg attgaagaca ccgggcgcga aagcagttac
     7981 gaaatgatga gggcgaaaga ttatctagac ctgctgattc cgcgcggcgg ggcggggctg
     8041 atacgggcgg tggttgaaaa tgccgtcgtg ccggtcattg aaacgggaac gggcatcgtc
     8101 cacatttata tcgacaagga cgcggattgg gacaaggcgc tccgtatcgt gtacaacgcc
     8161 aaaaccagcc gcccgtccgt gtgcaactcg atggaagtgc tgctggtgca tgaagacatt
     8221 gctgccgact tcctgcccaa gctcgaacgg ctgttggttc gcaaccgtat agaagccgga
     8281 ctgccgcccg tccgcttccg tttggatccg caggcggcgc ggcatatcgg cggcgaagcg
     8341 gcgggtgcag acaatttcga taccgagttt ttagactaca tcctcgccgt gaaaaccgtc
     8401 gcttcggtcg aagaggcggt cgggcacatc gaaacccacg gcacgcacca ttcagacggc
     8461 atcgttaccg aaaaccgcca cgctgcggac tatttcacga cccatatcga ttctgccgcc
     8521 gtgtatgtca acgcgtccac gcgctttacc gacggtggcg aattcggctt gggttgcgaa
     8581 atgggcatct ccacgcaaaa actccacgcg cgcggcccta tgggtttgaa agagctgaca
     8641 agttacaaat acatcgtaca aggcacggga caggttaggg aataatccga attcaaacgt
     8701 ctgatatagt ggattaactt taaaccgata cggcattgcc tcgccttagc tcaaagagaa
     8761 cgattctcta aggtgctgaa gcaccaagta aatcggtttc gtactatttg tactgtctgc
     8821 ggcttcgtcg ccttgtcctg atttttgtta atccactata aaaatgccgt ctgaaacagt
     8881 cttcagacgg catttccatt tgcaaaaaat caaacattgt ccaaggggac gagaatcgta
     8941 cggttgccgt tgtgttccgg tgcggacaca atgccttccg cctccatctg gtcaatcaga
     9001 cgcgcggcac ggttgtagcc gatacgcaag gcgcgctgta cgcccgaaat actggctttg
     9061 cgcgttttca ggacaacgga tacggcttcg tcgtacatcg gatcggtttc gccgtcgccg
     9121 ctgcgcccga tgccgggcag ctcttcgctg ccgccgccgc tcaaaatatc gtcaacatag
     9181 tccggctcgc caaactgttt caaatattcg accacgcggt gcacctcttc atccgaggca
     9241 aatgcgccgt gaacgcgctg cggataggca gtacccggcg gcaggaacag catatcgccc
     9301 tgaccgagca ggttttccgc gcccatttgg tcgagaatcg tgcggctgtc gattttgctg
     9361 gacacttgga acgcgatacg cgtcgggatg ttcgccttaa tcagacccgt gatgacatcg
     9421 acgctggggc gttgtgtggc aaggataaga tggatgcctg ccgcgcgggc tttttgggcg
     9481 aggcgggcaa tcagttcttc gattttcttg cctgccgtca tcatcaggtc ggcaaactca
     9541 tcaaccacga ccacgataaa cggcaatttt tccaaaggtt cgggattgtc gggcgtgagg
     9601 ctgaacgggt tgccgatttt ctcccccctt gctgcggctt cggcgatttt ttgattgaaa
     9661 cccgcaagat tacgcacgcc cataaagctc atcaggcggt agcgtttttc catttcgtta
     9721 acacaccagt tcagcgcgtt tgccgccagc ttcatatcgg taacgacagg ggcgagcagg
     9781 tgcgggatgc cttcgtaaat gctcaattcc agcattttcg gatcgatcat aatcatacgc
     9841 acgtcttccg gcgcggcttt gaaaagcata gacagaatca tcgcgttgac acccaccgat
     9901 ttgcccgaac cggtcgtgcc ggcaaccaac aaatgcggtg ctttgcccaa gtcggttacg
     9961 acgggctgtc cggtgatgtc ctgaccgagc gcgagcgtca gcttggattt ggattcggca
    10021 aactcgggcg aattgaagat ttcgctcagg cgtatcattt ggcgtttcgg gttcggaagt
    10081 tccaaaccca tacaggtttt gccggggatg gtttcgacaa cgcggatgga agccacgccg
    10141 agcgaacgcg ccaagtcttt ttccagattt agaacggaat tgccgcgcac gccgacatcg
    10201 ggttcgattt cataacgcgt aatcacgggg ccggaataag aatcgacaac cttgaccttg
    10261 actttgaact ccgccaattt ttcttcgatg gtgatgctgt tttccaacag ttcttcttcg
    10321 gtttgcgtcg cctcgggatt gaacagcgga ggcagaagca ggtcggttgt cggcaggtgt
    10381 tcgtataccg catcggtttc ttcagattgg aacgcccctt catccgcttc cgtatccgat
    10441 acccggcagg acgggcgttc agacggcaca tcttcaaacg gacaaatcgc ctgactgtca
    10501 tgcccgaacg cttcggtttc cgacagatat tgcccgtttg accgctctgc cgcaccttca
    10561 ctgccgtcat ctgcaatagc ggcggtttcc tcctgccaac ctccattcaa aacatcatcg
    10621 gcaagatggt cggtttcggc aatgcggctg cgttgcgcct gctcgaatcc tgacggcggt
    10681 tcataggtac ggttgtagat ttccgatacg ggaggcggcg gcggaatatc gattgcggtc
    10741 atgggaactt tcggcacttc gggtggttcg accacccaag catcgggcgc ggcaggttct
    10801 tcaatatgga tatcggcagt ttcatcataa accggaccgc cgtatcccac agacgaaacg
    10861 gtttccgtga aaacattttc ggtttcagac ggcatttcga catcccgttt cccgacaacc
    10921 gtccgacttt ccggaatcag gcgcgaaatc cgcgcctccg cagaaaccgt tgccttgttc
    10981 ggttctttca aattgacgga aacgcggcgg cgggcatcgc ggacggctgt gccgtctgaa
    11041 acggactgcc cttgggaaac atccggggat tttgcctccg cctgccctgc atgacggcgg
    11101 aacggcgcat tccgtgcatt ttcggctttg actgcagaaa aggcagacgg atgctgcttc
    11161 tcaaaatacg gctcaaatcc gtaatccgca gactcggaaa acgcttcttt gtccgcatcg
    11221 aacatacggg aatacgtgcg ttgaagcaca gggtcgtcca aaccgataat ctgcagccct
    11281 tccatcgggg tatcggacac gcggactttg gggaccgctg tttcgttttt ctcaaatgca
    11341 tcgatatagc gttttttcgt ttcccttaaa gccgcgctgt ttaaagcacg cgttgcttct
    11401 tccaaagtga tttccttaaa aaccggacgg acgggcgaaa tttcgctttc agacggcatc
    11461 aacccttcag caatactccg gtcgaatggg atgcggcggt tgtcgattac ggcagttgca
    11521 atgtcttccg tatcggcagc ctcttcctcc gcagcttctg cttcttccgt ttccgcttct
    11581 tccgtcccgt ttccactgtc tgaatacccg tcggatgcgt tcggaacatc cgcacggttt
    11641 gcagaagcag gcggaacgag cctgtccggt tcggattcgt gcgctaaata gtttcggcag
    11701 aaacggacga caccgacgaa cagccaatac actgccattt tgacggcatg gaaaagcatc
    11761 agggcaagtt cgggaaaatc gggcataccg tctttgattt caggcagctc cgcctgtttt
    11821 tcccctttct tttcctgcca cgcagaaacc tcgcgcatcc actcgcgttc ggattgtgcg
    11881 cggacaaaaa acaagccgac aagcgcaagc aacaaaataa cgatcaaaac tatccaaaac
    11941 atatgccgtt tcccgcaaga caaacgatta aacgggttta ttttaacgtg tttccatccc
    12001 aaaaaaaaca accggccggc aattctgccg aaaccgattc ggctttcggt ttgccccagc
    12061 cgacagtata atagccgtcc cttcccttaa tctttgcggc attgccatga ttttccaaac
    12121 agtctggttt tcagatatgg tactgtcggt ttcgtggatt gtccttatcc tgatactggc
    12181 agcttccgcg ccgtccgcat tccgctcgct tgccctctac cgttccgccc ttccactgtg
    12241 taccgtaatt ttttccgcag catggtgtct gaacgcctct gccggcggcg gacagcttgc
    12301 ccagatgaac taccacctgc tcgccgtcaa tctggttgca ctgatggtgg gcacttccgc
    12361 cgccctttgg cttgccgcgc tgctgatgct gccctactgc ctgctgtttg ccgattctgc
    12421 cggcgcatat ccgccgaacg ctttggtgct gattctgccc gcgctggtcg tcaaccgcct
    12481 gtcgcgtatg ctggtcaacc gcctgccgcc caatattttc atctttattt tcgtcaacgg
    12541 ctttctcgct tccgccgccg gcattctgtt gaccgggctg gtgctgaccg gtattttgga
    12601 tgctgcaaac gcttttccgt ccgaaatatt gtggacgacc gccctgcccg tctttattct
    12661 gctggcgtgg gcggaagcct tcctcagcgg catttcaact gccatatttg ttgcactgaa
    12721 gccccattgg attaacactt tcgacgacaa ccgctatctt aagtccgacc gcggcatatg
    12781 gcggtagcct tcagacggca tagacaggca aaaccatgct ttccaacatc atcccccttt
    12841 ccatcggtgc ggcactcggt gcaacggcgc gttggctgct caacctcgcc gttcctgccg
    12901 ccatgtctcc cgccacgggc aacctgtttg ccaactggac gggcgcgctg ctcatcggca
    12961 tctttgccga aaccatcaat cacccgcaat ggaagctgct gctgattacc ggttttctcg
    13021 gcagcctgac cacgctttcc ggattttcac tggaaaccgt aaccctgctc caatcgaatc
    13081 gtcctgcttc agcacttgcc aatattttcc tacacacggc aggttcgctg ctgctgactt
    13141 ggctcgggct gaagataggg gcggcagtca aataaccgca acaaattgcc tccctttgtt
    13201 ttgacataat ctataaatta gcttcgcttg tttaaatact ggctgtgcca attatagtgg
    13261 attaacaaaa atcaggacaa ggcgacgaag ccgc
//
LOCUS       DAXIEB010000054        13177 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000054 DAXIEB010000000
VERSION     DAXIEB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.054"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(117..770)
                     /locus_tag="ACFOA3_001223"
     CDS             complement(117..770)
                     /locus_tag="ACFOA3_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6898230.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(802..1314)
                     /gene="coaD"
                     /locus_tag="ACFOA3_001224"
     CDS             complement(802..1314)
                     /gene="coaD"
                     /locus_tag="ACFOA3_001224"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6898231.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1438..2121)
                     /locus_tag="ACFOA3_001225"
     CDS             complement(1438..2121)
                     /locus_tag="ACFOA3_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6898232.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2278..2544)
                     /locus_tag="ACFOA3_001226"
     CDS             complement(2278..2544)
                     /locus_tag="ACFOA3_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6898233.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2617..3087)
                     /gene="rlmH"
                     /locus_tag="ACFOA3_001227"
     CDS             complement(2617..3087)
                     /gene="rlmH"
                     /locus_tag="ACFOA3_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6898234.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            complement(3141..3527)
                     /gene="rsfS"
                     /locus_tag="ACFOA3_001228"
     CDS             complement(3141..3527)
                     /gene="rsfS"
                     /locus_tag="ACFOA3_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6898235.1"
                     /translation="MNEQELQDLQKMVEVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3586..4191)
                     /gene="nadD"
                     /locus_tag="ACFOA3_001229"
     CDS             complement(3586..4191)
                     /gene="nadD"
                     /locus_tag="ACFOA3_001229"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6898236.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDTVVFLPAGGPYH
                     KDAASTSAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4188..4787)
                     /locus_tag="ACFOA3_001230"
     CDS             complement(4188..4787)
                     /locus_tag="ACFOA3_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6898237.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGIDLSEGMETS"
     gene            complement(4816..6357)
                     /locus_tag="ACFOA3_001231"
     CDS             complement(4816..6357)
                     /locus_tag="ACFOA3_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6898238.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            6596..7981
                     /locus_tag="ACFOA3_001232"
     CDS             6596..7981
                     /locus_tag="ACFOA3_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6898239.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8001..8519
                     /locus_tag="ACFOA3_001233"
     CDS             8001..8519
                     /locus_tag="ACFOA3_001233"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6898240.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8858..9775)
                     /gene="thrB"
                     /locus_tag="ACFOA3_001234"
     CDS             complement(8858..9775)
                     /gene="thrB"
                     /locus_tag="ACFOA3_001234"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6898241.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     ADAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACQGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(9811..10539)
                     /gene="ubiG"
                     /locus_tag="ACFOA3_001235"
     CDS             complement(9811..10539)
                     /gene="ubiG"
                     /locus_tag="ACFOA3_001235"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6898242.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIVPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10691..11932
                     /locus_tag="ACFOA3_001236"
     CDS             10691..11932
                     /locus_tag="ACFOA3_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6898243.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNAFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            11940..12071
                     /locus_tag="ACFOA3_001237"
     CDS             11940..12071
                     /locus_tag="ACFOA3_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898244.1"
                     /translation="MPSEARRRLQTALPQQTATVFRHTAHYPAPHAQIPPRQTGTQP"
     gene            12082..13137
                     /locus_tag="ACFOA3_001238"
     CDS             12082..13137
                     /locus_tag="ACFOA3_001238"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6898245.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPGETADLRAKYGFADYETVFLFPSTGHTRKGLELLADFFEHTGL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDILASV"
BASE COUNT         3011 a         3985 c         3337 g         2844 t
ORIGIN      
        1 cttctgcagc agcgaagaac cgaactatac gcttgaagag atttgcagtc aaccgttttc
       61 ttgaaaaatt tttataaaaa tatttatcat attgtttata aattaattta tatttattaa
      121 cctgcttttg ccatataaaa ttcttccagc gcatgagaga tattttcagt ctgccattcg
      181 ttttttaagg gtgcaacaat ttcgatttgt cggttttggt aggcaaattg tattttccat
      241 gcatacagaa acatggtttc ggattctgtt ccgccgtata agctgtcacc cagaatcgga
      301 ctgcccaaac ttttcatcgc cactctcaat tggtgcgttt tgcccgtatg cggttccagg
      361 atgaacagcc gcagtttttc ggcgatactg atgctgtgga atcgggtaac ggcgatattt
      421 tctgtattgc gcgtcaactt ccacattccg cgtctggatt tttccattcc gcctttaatc
      481 caaccctgct ttttggacgg tttgcggtcg gatagtgcca aataggtttt tttgatgctt
      541 ttgccggcaa actgtccgct aagggcggac gcgctttctc tgttgagggc aaacagtaaa
      601 atgccgctgg tctgtttgtc caatcggtgc agcagccaca cacgctctac gcccaactgt
      661 atggcgagtg ttcgggccag tccggtctcg ccgctgtctt ggtggacgga tatgccgccc
      721 ggtttgttga tggcgacgaa gtcttgatgg cggaacaaaa tttccaacat atccatatat
      781 gccttgcaaa aatagaaggg ttcaattttc gtgttgatgt tcggcaagga ttttttcgta
      841 cacagcttgc ggcacgtagc ggtggatcgt ttccgtccag ccttccggcc cgaccagtcc
      901 tttgaccata gtggacgaca cttcggcgat ttcgcgcggc ggcatgagga atacggtgga
      961 tatttcgggg gcgaggtcgc tgttgatatg gcgcatggaa cgttcgtatt cgtaatccga
     1021 agcagaacgg atgccgcgca cgatgaatcc tgcatctacc tcacgggcgt aatgcaccag
     1081 aaatcggttt tcaaatacat cggttctgac gttgggaaac attttagtaa tatcgcacaa
     1141 catatcctgc ctttcagcga cggtataggt gctgcgtttg tcggggttaa tgccgatggc
     1201 gacgatgagt tcgtcaaaca tagattgcgc ctgccgtatc atccacagat gccccaatgt
     1261 gggcggatcg aaactgccgg cataaacggc gcggcgcggt gtattcggta acatttgatt
     1321 cctcccggct tcatagtcgg ctgtgtgttg gtgcgtgtgc atccgtattg tatgcccaaa
     1381 gtaaaatgcc gtctgaagca ttttcagacg gcatagtcgg acggcgtttt accggcatca
     1441 atcctcgccg tttaaagaca acaggatgtt cagcaggctg ctgaagatgt tgtaaagcga
     1501 gataaacagt gtcagtgccg cgctgatgtg gctgtcttcg ccgccgtcga tgacggtgcg
     1561 tacctgccac ataatcatta aggaactgaa caagacaaaa ccggcggaaa tggtcagggc
     1621 gagtgcggga atacccaaaa acagattggc aaccacggcg accatcagaa tgaccgcacc
     1681 tacggtcagg aagcgtccga gcgcgttcat atcgagccgg gttcggcgcg ccaaggcgga
     1741 catcgttaaa aagacggcgg cggtcatcgc ggcggcaatg ccgacgattt tcgcaccgtc
     1801 ggcaatatgg agcgcgtatt gcagcacggg gccgatcaat acgcccatac cgaatgtgaa
     1861 taccatcagc agggtaacgc cggtattgct gtaacggttt ttctcgatga agtggatcat
     1921 accgtagaaa aacgccaaca cgacggcaaa ccctatccag cgcgaaccga aggcggcgta
     1981 aaaattgaaa ccggcattgg cggcaagtgc cgcgcctgcg gcagccggaa tgaatgaaaa
     2041 tccgagcagg cggtaggttt tctgcaggac ggtgttttta gaaaccgtat gcgcggtgta
     2101 gtcgtaaacg tcgtgttgca tatcatctgc tcctgaaagc gcggttggga ataatggggg
     2161 attttaacat tgcccaatgt caaaatttgt ccggttgcgt gaagataaag ttgtccggcg
     2221 tattttaaag gccgtctgaa gcagtttcgg acagcctgtg ttcaaaacgg aaaaccgtta
     2281 ttgcggaacg tatccctgaa cggcatccgc gccgtcgccg aagaaatact gctccatctg
     2341 ctgagccagg tattcgcgcg cgcgcggatc ggcgaggctt aaacggtttt cgttaatcag
     2401 catcgtttgg tggcgcgtcc acgccgccca cgcttcttgc gatacgtttt caaaaatgcg
     2461 cttgcccaat tcgttgggaa gcggtggaaa tttcatgcct tcggcttctt tgttgagctt
     2521 gacgcagaat accatgcgtg ccatagcgga ttcctttgct gtgttcagaa ataacggggt
     2581 aattttaacc gattagggat acggacaaaa gccttcttat tcccgatgat agggatggtt
     2641 gtgcaggatg gaggcggcgc ggtagagctg ctcggtcaga aggacgcgca ccatcccgtg
     2701 cggcagggtc aggctggaca ggcgcatcat tatgcgggct tgttgtttga ggcggtcggt
     2761 cattccgtcc gcgccgccga tgacgaagca gacgtgttcg ccgttttgct gccaagtttt
     2821 gaggtgttcc gccagctcga cggaggtcgg tgctttgccg cgttcgtcaa ggacgacgag
     2881 gaacgcgcct tgcggtatgg cttcgaggat gcgtttttct tccgccgcca taccttgggc
     2941 ggcattcacg cccgcgccgc gtttttcggg tttgatttct ttgagtgcgt aggcgacgtc
     3001 gcgtccgaag cgtttggcgt attcggcgac ggcctcatca acccagcgcg gcattttggt
     3061 gccgactgcc aaaacggtaa tattcaatgc tttctccctt acaggaaaat gccgtctgaa
     3121 cattcagacg gcatcgggaa tcagtctgca gcgtgccacg gcttctgcat tccggcgtgg
     3181 aaactcggtt tctcgccgcc ccagagggtg tcgatgtcgt agaagtcgcg cacggcaggg
     3241 agcatgacgt ggacaacgag gtcgcctgcg tcaaccagcg tccattcgcc gctgtctcct
     3301 tcggtactga ggatttcaaa accggcttct ttcaaatcga cggcaacgtt gttggcaagg
     3361 gctttgactt ggcgcgtact gtcgccgctg gcgataatca ttctggcaaa cagcgaagtt
     3421 ttgtcttgcg tttcgagaac ggaaatgtct ttggctttga tgtcttcgag ggcgttgacg
     3481 gcgacctcga ccattttttg caggtcttgc agttcttgtt cgttcattat gttcctaacg
     3541 ggatgttttc agacggcatt atagccgttt cttactgatt tgactttatt tttcatacaa
     3601 accgtgttcg cggatgtagc gtgcggcggc aggcgggatg ccgtctgaaa cgccttggcc
     3661 ggcaaggttg cggcggattt ccgttgacga cacattatgc atcggggcgg acaagatgcg
     3721 gacgctgccg tcctgaaggg acttgcccag ccacgcgtgc agttcgcgcg gggtttggtg
     3781 caggctgtcg ccctgccgca tggcgacggc gatattggtt tcgcgcacga gcatctgcca
     3841 ttttttccat gtgtgcagct tcatcaggct gtcgctgccc atcagccacc aaagttgcgc
     3901 ggatgggaac tgctggcgga agatttggac ggtatcaaaa gtataggttg caccttctcg
     3961 gacgatgtcg caatcgctga cggcaaaacg cgcgtcttct gccgtcgcca gttcgaccat
     4021 ggcaaggcgg tcggcggcgg aagtggaggc tgcgtctttg tgatacgggc cgcctgccgg
     4081 caggaaaaca accgtgtcca acccgatttc atcggcaaag gcacgggcga tatgaagatg
     4141 cccgttgtgt atcgggtcga aagtgccgcc gaacagtccg attttcttca tgatgtttcc
     4201 attccttccg ataagtctat gccgtctgaa acactgccgt ccacaaccag cgtcagtttg
     4261 ccggtaacgg gatggatgac cagtccgtga acggcgatat ggcgcggcat cagcggatgg
     4321 ttacggataa ggtccaccgt gtggcgcacg ctgtcttcga cgttgtcgaa accggtcagc
     4381 cagccgtcga ggtcgatacc ggcataacgc agggtttcga tacggtcttc gggaatccgg
     4441 ctttcccgga cgcgcccgag gaattcttcg gcattcagcc cctgcatacc gcaatcgtga
     4501 tgggcgatga ccataatctc tctgaccttc agttcaaaca cggcaaccaa aaggctccgc
     4561 atcaccgaac cccacgggtg cgtaaccagc gcgccggcat ttttaatcag cttggcatcg
     4621 ccgtttttca aacccaacgc gtcgggcagc agcccgataa tccgcgcatc catacaggac
     4681 aaaactgcca gcccgcgttc ggggtatttg tcggtaaagt atttttcata ttcgcccgac
     4741 tcgacaaact gccggttatg ggcaaggatg ttatccaact cgctcatttt gccgtcctct
     4801 gaaaaagggt tcgcattata acgtttccgt ccgttttccg ccttcgccgc cgtccaacag
     4861 caggaaaata cccagcgcga acgccgccaa cagcaatgtc agcaccatcg cccgcgcgta
     4921 attatcctca cccgcgcgtc ccaaataggc ataaatcaaa gtcgtcagcg tctgccattc
     4981 cggacgcgac agaaacaatg tcgccgcaaa ttcgcccaca cacgtcgccg ccgccaaagt
     5041 cagaccgcgc cgcaacgccg gtttcaagag ggggaacgtg atgcggcatg ccgtctgaaa
     5101 gccgtttgca cccaaacccg ccgccgccct gccgtaatcc ggcggcagtg catcccaggc
     5161 tgataaaaca tcttttgcca caaacggata cgccagcagc gcatacatcg ccagcagcag
     5221 cggcaacgaa gccgtccact gcggataaag cagcagcacg cccgccgaaa cacaaaccgg
     5281 cgacaccata aacggcaaaa acatcagccc gcgcatccac gccgaccgcc gcgccgccgc
     5341 cgcatacacc acacccaaaa ccgccgccgc atacaccgcc gccgccgaga agcgcaaagt
     5401 attccacacc gcctgccacg tttcactttc cattaacaca cgccacgatt cgccggccga
     5461 ccacgctttc acaacaattg ccaacaaagg aaacaggcag cacacagaca acaccgccgc
     5521 cgcaaacgcc agcagcacat attccccgac cgactgcggc ggcgacggca tcacagggga
     5581 aaccgcctta tccgaaaccg cgcgcctgcc gaaccacgca tacagcaacc ctgccgccgc
     5641 cgttaccccc aacaccagcc acaccagcac cgaggcaacc gccatatcga gttcaaacat
     5701 gaccaactgg taaatttcca cttcgaccgt ggcataacgg ctgccgccca gcagcaatgc
     5761 cagcccgaac ccggaaaaac aatacaggaa gacaaggcac acgccgccgg caagccacgg
     5821 gcgcaaaacg ggcatttcaa tgtcccaaaa ccgccgccac gcccccgcgc ccaacgtccg
     5881 tgccgtctga agccgtgccg caggcacttg cacaaacccc tgatacgccg ccctgaccaa
     5941 cacaggaagg ttgaaaaaca cattgccgta caacaacaga tacggcgtat cctgccgtcc
     6001 gcgccacaac aggccgtccg ccccgaacag agccagcacg cccacgcccg ccaccaacgt
     6061 gggcatcaca aaaggcagca tcagcaggcg cagcaccaaa gcccgccccg gaaacgccag
     6121 ccgcgccagc acccacgcga caggcacgcc caaaggcagc accagcacac aggttgccgc
     6181 tgcctgaaat accgtccacg ccaaacgttt gagcatatag gcatccgaca gcaccgcgcg
     6241 ccacgccaaa ccgtcatacg ccgccaccgc ccacaaaggc gcaacgacca ttaccgccaa
     6301 aaaagccgaa ggcagcaggg caaaagcacc ccataccgcc caacgccgtc catccatcgc
     6361 cttccccact tgaaacactg atgttgcgat tgtacccaaa agccccacat accgtatatt
     6421 tcaatccgac tacataccgt atccgccttc ctcccgccgt ctgaaataaa tcgttcaaat
     6481 aaacaggaat ataacttcag acaaacaact taccgcccga tttgtgctat cgttttcgca
     6541 caacttaaaa aaacctgaca attttgtact tttattacag agaaaggctt tacaaatgga
     6601 cggctggaca cagacgctgt ccgcgcaaac cctgttgggc atttcggcgg cggcaatcat
     6661 cctcattctg attttaatcg tcaaattccg catccacgcg ctgctgacac tggtcatcgt
     6721 cagcctgctg acggctttgg caaccggttt gcccacaggc agcattgtca acgacgtact
     6781 ggtcaaaaac ttcggcggca cgctcggcgg cgtggcgctt ctggtcggcc tgggcgcgat
     6841 gctcggacgt ctggtcgaaa catccggcgg cgcacagtcg ctggcggacg cgctgatccg
     6901 gatgttcggc gaaaaacgcg caccgttcgc gctgggcgtt gcctcgctga ttttcggctt
     6961 cccgattttc ttcgatgccg gactaatcgt catgctgccc atcgtgttcg ccaccgcacg
     7021 gcgcatgaaa caggacgtac tgcccttcgc gcttgcctcc atcggcgcat tttccgtcat
     7081 gcacgtcttc ctgccgcccc atccgggccc gattgccgct tccgaatttt acggcgcgaa
     7141 catcggccaa gttttgattt tgggtctgcc gaccgccttc atcacatggt atttcagcgg
     7201 ctatatgctc ggcaaagtgt tggggcgcac catccatgtt cccgttcccg aactgctcag
     7261 cggcggcacg caagacaacg acctgccgaa agaacctgcc aaagcaggaa cggtcgtcgc
     7321 catcatgctg attcccatgc tgctgatttt cctgaatacc ggcgtatcgg ccctcatcag
     7381 cgaaaaactc gtaagtgcgg acgaaacctg ggttcagacg gcaaaaataa tcggttcgac
     7441 accgatcgcc cttctgattt ccgtattggt cgcactgttt gtcttgggac gcaaacgcgg
     7501 cgaaagcggc agcgcgttgg aaaaaaccgt ggacggcgca ctcgcccccg tctgttccgt
     7561 gattctgatt accggcgcgg gcggtatgtt cggcggcgtt ttgcgcgctt ccggcatcgg
     7621 caaggcactc gccgacagca tggcggattt gggcattccc gtccttttgg gctgtttcct
     7681 tgtcgccttg gcactgcgta tcgcgcaagg ttcggcaacc gtcgccctga ccaccgccgc
     7741 cgcgctgatg gctcctgccg ttgccgccgc cggctttacc gactggcagc tcgcctgtat
     7801 cgtattggca acggcggcag gttcggtcgg ttgcagccac ttcaacgact ccggcttctg
     7861 gctggtcggc cgtctcttgg acatggacgt accgaccacg ctgaaaacct ggacggtcaa
     7921 ccaaaccctc atcgcactca tcggctttgc cttgtccgca ctgctgttcg ccatcgtctg
     7981 acagacggaa aggatagtaa atgactacgc attttgtcgt tatgggcgta tgcggctgcg
     8041 gcaagaccac cgccgcgctg tccctgcaga aacacctcgg tcaatgtccc tatgccgaag
     8101 gcgacgagtt ccacacccaa gccaaccgcg acaagatggg cgcgggtatt ccgctgaccg
     8161 atgaagaccg ctatccgtgg ttgggcaatc tgcgcgactg gatgacgcaa caggcgcaaa
     8221 acggtgcgaa ccacaccatc gtaacctgtt ccgccctcaa acgcggctac cgcgacattc
     8281 tgcgcggagc cgaaggcaaa gctgccttca tccacctcag tccgccgcaa gacatcaacc
     8341 tcgagcgcat gatgtcgcgc aaaggacatt acatgaaagc agggatgctc gattcgcaac
     8401 tggaaatcct cgaggaactg ggcgaaggcg aatacggggt caaaatcgcc aaccccggca
     8461 cgcccgaagc ggtcgaagcc gatattctga actgggttgc ctcggaaaac ctgctttgaa
     8521 gcaatatgcc gtctgaagcc cgacacagga tgggtttcag acggcataaa catcgggaac
     8581 agaatggatt acattgattt atagtggatt aacaaaaacc agtacagcgt tgcctcgcct
     8641 tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
     8701 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataaaatgg
     8761 aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac
     8821 cctttcagac ggcatttctg tcaaacgccg gacgcactca acccaaactc aacagcaggt
     8881 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaagt
     8941 ccaacaggcg cgacacccaa aaacggatgc agccggcacg ttgggcggtc gggaaatacg
     9001 ccttttcttc ggcactcaag gggcgcacgc cctcataacc accgataaac gcctttttca
     9061 acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
     9121 catacataaa attgccctgg caggcataat aaaaatcgat gaagcccgat acctgaccgc
     9181 cgtcaagcaa cacattgtct ttaaacaggt cggcgtggat gatgcccgaa ggcagatgat
     9241 tgccgagatt gtccttcagc gcatcgattt cggaacacag cagcgcggca tcggcttgcg
     9301 acaggacagg cagcagccgg gcgcacgcct ccgtccacca cgcatcgtaa cgcgggtttt
     9361 ccatttccaa agggaaatcg gcggcggcaa ggtgcatttt cgccaacatc gcaccggtat
     9421 ggaaacactg ctcagccgtc ggcagcgcgg tatccgaacc tttcaggcag gcaactaggc
     9481 aggcgggctt gcccgccaaa acggaatcaa gccggccgtc tttgcgcgca accggcgcgg
     9541 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
     9601 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
     9661 aattgctgtt ggtaatcccc tgcgcgatgc cctgcaggga aacaaattcc cccaaatcgt
     9721 aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtataaaca gacatatcca
     9781 ttcacccgtt caaaacaatc aaacccgccg tcagaacgcc ggacggcagg caaacatata
     9841 attcacatcg gtcgaatcgc acagggcata agtttgcgac aaaacatggt aagtcatacc
     9901 cttcgtatcc gccacatcca agcccgcctg acggcacatc cgcgccagct cggcaggcac
     9961 gataaatttt ttccagtcgt gcgtgccttt ggggacaaac ttcaacagat attccgccgc
    10021 cacaatcaga tgcaggtacg atttcgggtt tttattgatg gtggaaaaaa acaccatgcc
    10081 gtccggtttg accagcttgg cacaagcacg cacgatggcg gcgggatcgg ggacgtgttc
    10141 catcatttcc atgcacgtta ccacatcgaa cgagtgcggt tccgcctcgg caaggtcttc
    10201 cacgcggata cattcgtatt cgatatcggc gacattgttc aaagccgcgt gcaggcgggc
    10261 ggtttccagc gactgctccg ccatgtcgat gcccttgaca aacgccgcgc cgcgccgcgc
    10321 catgctttcc gccaagatgc cgcctccgca gcccacgtcc aaaacccgtt tgccgcacaa
    10381 atccgcgtgt ccgtcgatat aatcgagccg gagcggattg atgtcgtgca aggttttgaa
    10441 ctcgcccgac ttgtcccacc atttgtcggc aatccggctg aatttggcga tttccccctc
    10501 atcgacatta tattttttgt cggacatttt ccctcccatc tgacgaaccg cccactccaa
    10561 aacccaagat tatactatgg aacacaatgc cgtctgaaag ccttttcggg cggcgcggcg
    10621 caaatgcctt acaaatcctt acactttacg gcataatggc ggctcgcttt ttctggcaga
    10681 aagacaaaat atgcccacca aaaccccttc actgttcggc ggcgcgatga ttatcgccgg
    10741 cacggtcatc ggcgcaggta tgctcgccaa cccgaccgcc acatccggcg tatggtttac
    10801 cggctcgctg gccgtgttgc tgtacacctg gttttctatg ctttccagcg gcctgatgat
    10861 tttggaagtc aacacccatt atccgcacgg cgcaagtttc gacacgatgg tcaaagacct
    10921 gctcggacgc ggctggaaca tcatcaacgg catcgccgtc gccttcgttt tatacctgct
    10981 cacctacgcc tacatcttcg ttggcggcga cctgaccgcc aaaggcttag gcagcgcctt
    11041 gggcggcaat gtttcactca ccgtcggaca actcgtcttc ttcggcatcc tcgccttttg
    11101 cgtatgggca tccgcacgct tggtcgaccg cttcaccagc gtcctcatcg gcggcatggt
    11161 attaaccttt atttgggcaa ccggcggcct gattgccgat gccaaaatgc ccgtcctgtt
    11221 cgacacccaa gcccccgtcg gcaccaacta ctggatttac gccgccaccg ccctgcccgt
    11281 ctgccttgcg tcattcggtt tccacggcaa cgtctccagc ctgctcaaat actttaaagg
    11341 cgacgcgccc aaagtggcta aatccatctg gacgggcaca ctgattgcgc tggtaattta
    11401 cgtcctctgg caaaccgcca tccaaggcaa cctgccgcgc aacgcgttcg cccccgtgat
    11461 tgccgccgaa gggcaagtct ccgtcctcat cgaaaccctg tccaaattcg cccaaaccgg
    11521 caatatggat aaaatattgt ccctgttttc ctatatggcg atcgccacct cgtttttagg
    11581 cgtaacctta ggtctgttcg actacatcgc cgacatcttc aaatggaacg acagcgtgtc
    11641 cggccgcacc aaaaccgccg cgctgacctt cctgccgcct ctaatttcct gcctgctctt
    11701 ccccaccggc tttgttaccg ccatcggcta cgtcggcctg gcggcaaccg tctggacagg
    11761 catcatcccc gccatgctgc tctaccgttc gcgcaaaaaa ttcggcgcag gcaaaaccta
    11821 taaagtttac ggcggcttgt ggctgatggt ttgggtcttc cttttcggca tcgtcaacat
    11881 cgccgcacag gtattgagcc aaatggaact cgtccccgta tttaaaggat aaaggcaaaa
    11941 tgccgtctga agcccgccgg cggcttcaga cggcattgcc gcaacaaacg gcaaccgtat
    12001 tccggcacac agcgcattac cctgcccctc acgcacaaat cccgccccga caaaccggga
    12061 cgcaaccata aaggaacaat gatgaagctc aaaatagaca ttgcaaccaa caacttcaaa
    12121 cacggcggcg gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac
    12181 atcacaccgg ccgtttatgc gacgaaattt gatcacggca ttcctgaata cgccatgatc
    12241 gaaccccatc ttgtcgatca acaccggacg ctgaaaaaac tacgctcatt cctcttttca
    12301 agccggctcg ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc
    12361 gattacgccg acctcctcat ctgcggcggc acacacttgg gctacctgca ccatatggcg
    12421 caaaaaccga acctgctcga ccgcctcgcc atacgccgta accgcagcaa ctacgccacc
    12481 gccaaactga ttatggcgca ttcccatatg atgcggcgcg aactggtcgg actgtacggc
    12541 gttccccctg aaagaatcca agtcgccccc ccccccgcag atacggaacg cttcttcccg
    12601 caacccggag aaactgccga cctgcgcgcc aaatacggct ttgccgacta tgaaaccgtt
    12661 ttcctgttcc catcgaccgg ccacacgcgc aaaggtctgg aactgcttgc cgactttttc
    12721 gaacataccg gcctgcccgt caagctcgcc gttgtcggct ccccgctccc ccgccctatg
    12781 aaaaacgtcg tcgggctggg cttctgcacc gatatgcccg aactctaccg cgccgccgac
    12841 tttaccatta tggcctccct gtacgaaccc ttcgggctgg tcggtgtcga atccgtccta
    12901 tgcggcacac gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa
    12961 gccggattct ttttctcacg ccaaaacccg gaaaccctgg cgcaagccgt tgcccaagcc
    13021 gtcagcctta aaaaacaggg cggacaccgc ctgtccgacc cgatgcgtgc actgaactac
    13081 aacccgtccc tgtcgcacca catcgaccga ctgaccgaca tactggcatc cgtataacaa
    13141 aatgccgtct gaacgagttt tcagacggca ttgctgc
//
LOCUS       DAXIEB010000055        12907 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000055 DAXIEB010000000
VERSION     DAXIEB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.055"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(95..403)
                     /locus_tag="ACFOA3_001239"
     CDS             complement(95..403)
                     /locus_tag="ACFOA3_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898246.1"
                     /translation="MTNNVDLKVPVKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(387..689)
                     /locus_tag="ACFOA3_001240"
     CDS             complement(387..689)
                     /locus_tag="ACFOA3_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6898247.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(763..3126)
                     /gene="pheT"
                     /locus_tag="ACFOA3_001241"
     CDS             complement(763..3126)
                     /gene="pheT"
                     /locus_tag="ACFOA3_001241"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6898248.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIALVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3162..3680)
                     /locus_tag="ACFOA3_001242"
     CDS             complement(3162..3680)
                     /locus_tag="ACFOA3_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898249.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3687..4751)
                     /locus_tag="ACFOA3_001243"
     CDS             complement(3687..4751)
                     /locus_tag="ACFOA3_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6898250.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4928..5920)
                     /gene="pheS"
                     /locus_tag="ACFOA3_001244"
     CDS             complement(4928..5920)
                     /gene="pheS"
                     /locus_tag="ACFOA3_001244"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6898251.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6266..6625)
                     /gene="rplT"
                     /locus_tag="ACFOA3_001245"
     CDS             complement(6266..6625)
                     /gene="rplT"
                     /locus_tag="ACFOA3_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6898252.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6638..6835)
                     /gene="rpmI"
                     /locus_tag="ACFOA3_001246"
     CDS             complement(6638..6835)
                     /gene="rpmI"
                     /locus_tag="ACFOA3_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6898253.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6982..7503)
                     /gene="infC"
                     /locus_tag="ACFOA3_001247"
     CDS             complement(6982..7503)
                     /gene="infC"
                     /locus_tag="ACFOA3_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6898254.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7521..9434)
                     /gene="thrS"
                     /locus_tag="ACFOA3_001248"
     CDS             complement(7521..9434)
                     /gene="thrS"
                     /locus_tag="ACFOA3_001248"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6898255.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VDEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWEKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RVELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9488..9564)
                     /locus_tag="ACFOA3_001249"
     tRNA            complement(9488..9564)
                     /locus_tag="ACFOA3_001249"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9528..9530),aa:Val,seq:gac)
     gene            9743..10858
                     /gene="tgt"
                     /locus_tag="ACFOA3_001250"
     CDS             9743..10858
                     /gene="tgt"
                     /locus_tag="ACFOA3_001250"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6898256.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIEQFGGLHELIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFQSPINGDKLFLSPEVSMKIQTVLNSDIVMQLDECTPGEATHSQAQKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRSYLHHLHRVGEILGAQLNTIH
                     NLHFYQVIMAEMREAIEQGKFADWQARFHENRARGVD"
     gene            10868..11950
                     /gene="hemH"
                     /locus_tag="ACFOA3_001251"
     CDS             10868..11950
                     /gene="hemH"
                     /locus_tag="ACFOA3_001251"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6898257.1"
                     /translation="MPSKAASDGILSLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12142..12543
                     /locus_tag="ACFOA3_001252"
     CDS             12142..12543
                     /locus_tag="ACFOA3_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6898258.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNNIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12606..12896)
                     /locus_tag="ACFOA3_001253"
     CDS             complement(12606..12896)
                     /locus_tag="ACFOA3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898259.1"
                     /translation="MAKYFTETDIGIREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3296 a         3401 c         2840 g         3370 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactataa ttatttttta gcgtgtaaaa
       61 caaaaccggc tgcatacctg caaccggcct caaatcagca caattcctta tccaaatccg
      121 ccaacagctc tttcagaccg tctccgattt cctgaagcgt aaccggacgg ttgccgtccg
      181 aacccgactc cgcaccttct gcatacggtg caaacgtgct ctgcaatttc aacagtttca
      241 ccacatccaa acgggtgtag cgttcgccgc cgtaaccgac aaccacaccg tgatcatgct
      301 gccattgcgc aaaaccatag gggctgattt gcaacagtcc gcacaactcg tccagcgtga
      361 aatagcgttt tacgggaacc ttcaaatcaa cgttgtttgt catagtagtg ttccaccatg
      421 cttttgagtt tctggctggc atggaaagtt accacgcggc gggcggtaat cggcacttcc
      481 tcgccggttt taggattacg acccgggcgt tgcggcttgt cgcgcaactg gaaatttccg
      541 aaaccggaaa ttttgatttc ttcgccgctt gccaaagtgc tgcggatttc ttcaaaaaag
      601 agttcgacga tttctttggc atcgtttttg gtgacgttgc tgactttgtc taccaaaata
      661 tcggccagtt ctgctttagt gagagtcata tgattacctt ctgttgtaac agttgtggat
      721 tattaccaat ttttatttaa aattcaagcg aatatttatt ttttaactgc gaagccgcgc
      781 ccctgccgcc gttgccgcgc caatcagttt tccgataagc ggctcgactg cctcatccgt
      841 cagcgtgttt tccatatcct gcaaaatcac tttgaccgcc acgctcttca tcccttcggg
      901 cagtcccgtg ccgcgataca cgtcaaacac gctgatttcc tgtaccaact tgtttgccgc
      961 gcctttcaag acaagcagca aatcatcatg gctcatagct tccggcatca caaacgccaa
     1021 atcgcggcgc accggctgga atttcgatac gacccgatag cgcgttttcc cgcattccaa
     1081 cacggccgcc atatcgattt caaataccag cggcgcttgc ggcaggtcgt atttttgcag
     1141 ccatttcgga tgcagttcgc cgacaaagcc gatgactttg ccgtctgaaa cgatattggc
     1201 ggcacgtccg ggatgcaggg cgggatgtcc ggttttaacg aactcgactg ctttgttttt
     1261 caacagattt tccacgtccg ccttgatgtc gtaaaaatcc gcattgcgcg ttttcccgcc
     1321 ccattgttcc ggcatgaccg cgccgtacca caatccgccg atgcgttcgt tttggacaaa
     1381 ctggccgtct gaacctttgc tgaacacgag ggcgatttca aacacgcaca cgcggttttg
     1441 tttgcggttc agattgtttt gcagaatttc caccaagccg ccgatgagcg tggaacgcat
     1501 cacggcatac tgcgccgcca gcgggttttg caggcggatg gggtcggcgt tggcggcaaa
     1561 atcttgttcc cactgctcgt caacgaaggc atagctgacc acttcgcggt aaccgcgagc
     1621 cgccatttcg ttgtaaacgg caaaacgcgg gcggcgtgtt tcgggcagtt ccagcatttt
     1681 cagacggcct gacgtgtaat cgtcggggat gttttcatag ccgtaaacgc gtccgatttc
     1741 ttcaatcaaa tcagcctcaa tttcgatgtc aaaacggaag ctcggcgcgg taacgcggaa
     1801 gccttccgcc gttttctcgg gctgcaggcc caagtgttgc aaaatggttt ccacctgttc
     1861 ggcaggaatg tccacgccca acacggtttt cagacggtcc aaacgcaatc caacctgctt
     1921 cgcttcaggc aattcgcctt gcgcttccac catctcgcct gccgcaccac cgcaaatctg
     1981 caacaccaat tcggtagcac gttcaatggc atccgcctgc aaacggtaat ccacgccgcg
     2041 ctcgaagcgg aacgacgaat ccgaaccgaa accgtattgg cgcgatttgc cggcgatgat
     2101 ttcgggcgca aaccaagccg cttccagcac gatattttgc gtgccgtctg aaaccgcgct
     2161 cgccgcgccg cccattaagc ccgccaaact caacacgcct ttttcgtccg ccacgaccag
     2221 cgtgttttca gacagggaaa cggttttctc gttcaggcat tccagcgttt ccccttcgcg
     2281 cgcgcggcgg atgtgcaggc tgccggaaag tttgtcggca tcaaaaacgt gcatcggctg
     2341 accgatttcc agcatcacat aattgccgat gtccaccagc gcggaaatac tgcggatgcc
     2401 gctgcgctcc aaacgttgtt tcatccaatc gggagtagcc gctttcgcat tgacattttc
     2461 aataacgcgg ctgataaaac ggccgcaatc ggcaggcgcg ttaatctgca cgggctgttt
     2521 tcgactgccc gtgatcggcg cggtatggat ttcgggctgc ctgaacgcgc accccgtcaa
     2581 tgcggacact tcgcgcgcaa tgcctttgat gctcaagcag tcggcgcggt taggcgtaat
     2641 tttcaacgta aacagcgtat cgtccaaatc caagtattcg cggatattgg taccgacggg
     2701 cgcatcttca ggcagaatgt gcaggccgtt cacaccgtcg tcgggcagac cgagttcgtc
     2761 ggtggaacac aacatcccgt ccgacaccac gccgcgcatt ttggtcggct tgattttgaa
     2821 attacccggc aaaacggcac ccggcagcga acacggcact ttgatgcccg ctttcacatt
     2881 cggcgcaccg cacacaatct gcaccaaccc gcccgtaccc gcatcaactc gggtaacgtt
     2941 caaacggtct gcatccggat gtttttcaac ggatttcact tcggcaatca ccacgcccgc
     3001 aaacgcaggc gcggcagttt cggcctcttc cacttccaag ccggacatcg ttaacagatg
     3061 ttccagctta tcggaggaaa gttcggtatc ggcttgggtt ttcagccatg agtaggagaa
     3121 ttgcatgtta tgttctcaat taactaatta actttagatt ttcaagagct agctaaacct
     3181 ttttgaatgg ctaagtccct attaattact tctacagaaa tgttccataa agaaatcaac
     3241 cgcaaagtat ctttcttatt aaattcttgc tttattaatt cattcaattg cccaagataa
     3301 ttaagcggca catccgggat aaccagtaat aatttgattt ctatgctacc ccattgcaaa
     3361 tttgaaaaag cactaggcat aattgtttct ttgatatctt ctctgcctaa tattcccaaa
     3421 atttcaacat ttaatgcatt acaccatttt tgataaatct ctgaaatata ttcatcaaaa
     3481 cgctcaggag atctttcttg atttggaata ctttgttttg cctcaacaaa taacaaatta
     3541 tcttcttgca gatagataaa ctctactgtc ttaatacctt cttgccttct cttccggata
     3601 tatggttcta tataaaacag gttatttttt actagattaa aagttatacc tgattcagtg
     3661 agatttatta tttcactcat aaaaacctaa ccaatccatg ctgtttccgg agataaaaca
     3721 cctatttctt ctctgaacaa tcgaatagac tcaccaataa tttcattatc cggcatgcta
     3781 ttttgtaaat tttcctgcac ccaagtagtc tgttcctttt cttttccagg cataaaaaca
     3841 ggaatacttt cactatgctt tctagctaat aaacacagct tcttgataac aaaatatgaa
     3901 tgcgtactca taaaaatctg tattcccaat ttggatttag ataaggaaaa cagaatttct
     3961 agaaattgat taattgcttt cggatgtaga ttagcttctg gctcatcaat aaataagatt
     4021 gatccaggac tcaaaaaccg atttcctaat agagtgtcta atatcccaat cttttttacc
     4081 ccctctgctg tagaattgat agagaaagtt tgtctatctt tttcatatac ccatgccttc
     4141 tttttatcat tgtagctaac ataaccgcca aacaatgctt ctagatcctt tctagcttcc
     4201 gctgcctcag ggtaattcct cccttttgtt ggtttaatat ccaatgcttt taccaaatca
     4261 atgtaagtag catcaaaacc aaatctcttt tcttcctctg attttttaat tacatcaaat
     4321 agtgatagaa cttctttggg tggtaaaaaa atagagttag cgtttattct gggagaaagt
     4381 tcgtatagct ccggtttaat tagctttttg gtagttctac caaaagaaaa caaaactgaa
     4441 gaactatctt ctaaagtcat acttaactta agaggccgtt cgtttggatt ttcttttcct
     4501 cttgtaacta aatcaccaat ttcctctact tggaacgtcc aatatagctt atctcgcaat
     4561 tcctcagaaa aatcacgaat attctttcct ttatgagctt cctcatggct ttttaaaaca
     4621 ctatataatg cctttaaaat aaaagttttc cctttgctat tttctgcaat aattaaattt
     4681 attttcccta aattatcagc cttcaataag ctaatcggcc cgaaattttc aagccaaaat
     4741 gatttaatca tataatcccc ctaatatata tcaacaatat ttgtacaaga cacattgtga
     4801 aaagacagca accacagcac catagtaaac gcatagcagg cggataatgg agcgacatag
     4861 cgtttcatga tttagctttc tgaggtaggc acaggctgct tttttcattt ttaggcagcc
     4921 tacacaatca ttttacaaac tgcttcaaaa aattcaaatc gttatcaaaa aacaagcgca
     4981 aatcgttcac gttgtaacgc agcatcgcga aacggtcgag accgataccg aaagcgaaac
     5041 cggtgtattt ttcgggatcg atattcacat ttctcaacac gttgggatgc accataccgc
     5101 agccgccgac ttccagccat ttgccgtttt cgcccataat gtcgatttcg gcagacggtt
     5161 cggtgaacgg gaagaaagac gggcggaaac gtacttgcaa atcatcgcgt tcaaagaagc
     5221 gacggataaa atccgtgaac actgctttta agtcggcaaa agttacgccc tcttctaccc
     5281 acaaaccttc cgcctgatgg aacataggcg agtgcgtggc atcgctgtcc acacggtaaa
     5341 cgcggccggg ggcgataatg cggatgggcg gctctttttt atcgagcata tagcggattt
     5401 gaatcgggga agtgtgcgta cgcaaaacat caccgttttc aacgtaaaac gtatcctgca
     5461 tcgcacgggc aggatggttt gcagggatgt tcagggcttg gaaattgtga aaatcgtctt
     5521 cgatttcagg cccgtccgcc acttcgaaac ccattccgtg aaagagttcg accacacgtt
     5581 gcaaggtcag ggttacggga tgcaggctac cgccttcctg agcgcgtccg ggcagggtaa
     5641 tgtcgagggc ttcggcggca agtcgggctt gcagcttggc ttcgttgagg gcatcgcgtt
     5701 tggcattaaa agccgtctga aaccggtttt tgcattcatt gatatgcgca cctatggttt
     5761 tgcgctcttc aggcgacatt tgccccaaag ttttcagaag tccggtcaac tcgccggttt
     5821 tgccaagata acgggctttg atttgttcta gagcgttgaa gtcttgcgca gcttctactg
     5881 cggcaatgcc ttctgcaacg atgcggttta cattttccat aatatcaagt ctgtcaatta
     5941 atgatatgcc acctgaaaca acgaggccgc ctgtatagcc tgacatatca gactcttcag
     6001 acgaccttat tttcttcacg gtcaatccat tggataaatt ttctgtatag tggattaaat
     6061 ttaaaccagt acggcgttgc ctcgccttgc cgtactatct gtactgtctg cggcttcgtc
     6121 gccttgtcct gatttaaatt taatccacta tattttaacg caaatttatt taaaaaaata
     6181 gactttaaag aaagaaaatc aatcacatag aatttctgca aacaaagaaa aaaggaagcc
     6241 gcagcttcct tttcaatttt ttggattaag cagccaaagc agctttggct ttttcaacca
     6301 attgtgcaaa agcggcttta tcgaacacgg ccaaatcagc caatactttg cggtcgattt
     6361 caatagaggc gcgtttcaga ccgttcataa atttgctgta agacaacccg ttttcacgcg
     6421 tacctgcatt gatacggaca atccacaatt gacggaattg gcgtttgcgt tggcggcggt
     6481 cacggtatgc gtattgaccg gctttcatta ccgcctgctt ggcaacgcgg taaacgtttt
     6541 tacgacgacc gcgatagcct ttggctaacg cgaagatttt ttggtgacgg gcacgggcgg
     6601 taacaccgcg ttttacgcgt ggcatattct aaactcctta agcgtagggt aacattttag
     6661 caacagaagc caaatcgcga tcatttacca tagaggtacc gcgcaattgg cgtttgtttt
     6721 tggtggtctt tttagtcaag atgtggcgtt tgaacgcatg agcgcgtttc acaccgccgt
     6781 tacccagtac tttaaagcgt tttttcgcgc tagacttggt tttcatttta ggcatgggaa
     6841 aactccattc gttatcggat aaggcattag ggggttttaa aacatcggtt tcaaacactt
     6901 gaacccacaa tgccacggtt ttttgccgca attaaaaact tactccgaag cgacaatcgg
     6961 agtaagcgga gaattatagc tttatttttt cttcggcgca atcatcatca ccatttggcg
     7021 accttccatt ttgggaaagg actcgatttg cgccacttca gccaaatctt cttttacacg
     7081 ttccaaaagt tgcgcgccga gttgctggtg agccatttca cggccgcgga aacgcaatgt
     7141 cactttgact ttatcgccgt cggcaaggaa gcggttgatg ttgcgcatct tgatttgata
     7201 atcgccctca tctgtacccg gacggaattt gatttccttg atttgtacct gcttttgatt
     7261 tttcttggct tcgtcgcgtt tcttggcctg ctggtatttg tatttaccgt aatccatcag
     7321 tttgcacaca ggcggtttag cagttgggga aatctctacc aaatcgacat cctgcccttc
     7381 ggccatagcc aaagcttcac gaactgaaac gacaccaagc tgttcgcctg actcactgat
     7441 taaacgcact tctttggcgg taatttcgcc gttgattcgt gcttcgcgtt cttgagcgat
     7501 gatgaatact cctataaaaa ttaatgattg acgagggcat cagtgatttc ttgctgcaat
     7561 tgcgcaatga aatcatccaa atccaaagaa cccaaatctt ctgctttgcg gcgtaccgcc
     7621 actttgtttt cctgcttctc cttatcgccg acaacgattt gataagggaa acggtattgg
     7681 ctgttgtcgc ggattttgta accgattttt tcgttacgca aatccagctc tacacggaag
     7741 ccagcggctt gcaatttagc agccacttcc cgacaataat ctgcctgatt ttcggtaata
     7801 ttcataatta ccaattgaac cggagccagc cataacggga aagagcctgc atggttctca
     7861 atcagaatgc cgataaaccg ttccaaagaa cctaaaatgg cgcgatgcaa cataacaggt
     7921 cgcgcacggt cgttgttttc ggttacatat tcggcattca aacgctctgg caatacaaag
     7981 tccaactgca gggtaccgca ctgccaagag cgacccaagg catctttgac atgatattca
     8041 atcttaggac catagaatgc gccttcgcca ggcaactctt cccactctac gccgcaagcg
     8101 gtcaaggcat cacgcagccc ttgttcggct ttttcccaaa cttcgtcaga acctgcacgt
     8161 tgttcagggc ggagggacaa cttcacggca acatcatgga agccgaattg tttgtaaatg
     8221 cgaaccagca attcattgaa tgcacgtgcc tcatcaacga tttgatcttc ggtacagaag
     8281 atgtgcgcat catcctgtac aaaaccgcga acacgcatca gaccatgcaa tgcaccgctt
     8341 ggttcattac ggtggcaaga accgaactca gccaaacgca ttggcaaatc gcgatatgag
     8401 cgcaagccgt tattaaaaat ttgcacatga cccggacagt tcatcggttt caccgcgtat
     8461 tcgcgttttt ccgaactggt tacgaacata ttgtctttgt agttgtccca atggccggat
     8521 ttttcccaaa aggttttatc catgatttga ggcgttttga cctctttata accggcggcg
     8581 ttcagttctt tacgcatatg ctgttcaatc acttgccaca aagcccagcc tttaggatgc
     8641 caaaacacca tgcccggcgc ttcgtcttgc agatggaaca gatccaattg cttgccaagc
     8701 ttgcggtggt cgcgcttttc ggcttcttcg atacgttgaa tataggcttt caactcgtct
     8761 tttgtcgccc aagccgtacc gtatatgcgt tgcagcattt cattgttgct gtcgccgcgc
     8821 cagtatgcgc ccgccaactt ggtcagtttg aagtttttca ggaaacgggt attcggaacg
     8881 tgcggtccgc ggcacatatc gacatattcc tgatgatgat acatccccat cgcttccact
     8941 tcgggcatat cgtcaatcag gcgcagtttg tattcttcgc cgcgctcttg aaaaatttta
     9001 atcgcctccg cacgcggagt catgattttg accacatcat agtcttgggc aatcaattct
     9061 ttcatacgcg cctcaatagc ggcaacatct tccggtgtaa acggtttttc cgtggcgatg
     9121 tcgtaataaa agccctcttc aatgacgggg ccgataacca tttttgcatt aggatagagt
     9181 tgcttgacgg catgcccgac aagatgcgcg caggaatggc ggatgatttc gatgccttcc
     9241 tgatctttcg gagtaatgat ttgaacagca gaatcttcaa caatcgggtc gcacgcatcg
     9301 accaatttgc cgtttacctt gcctgccacc gtcgccttcg ccaaaccggc accgatagac
     9361 gcagcaattt gagccacggt aacgggggat tcgtattggc ggactgagcc gtccggcaag
     9421 gtaatattca acatcaaaaa gctccaaaac attaaaaaat aacggcataa atgccgttat
     9481 gggaatttgg taggcacgat tggattcgaa ccaacgaccc ccaccatgtc aaggtggtgc
     9541 tctaaccaac tgagctacgt gccttcaaag aatttttgca ttttatcggg tcgttttaat
     9601 ttttgcaaga gatatgtgca tttctttatg caacattgcg gcaagatttg gggatgccgt
     9661 ctgaaaaacc gtgggaaatc atttataatg ccgtctttta aaatatattt cgacccctgt
     9721 cgcccaactg acattcaatc ttatgcttaa atttacttta cacaaaaaag acggatacgc
     9781 ccgacgcggc acgctggagt tgaaccacgg caaaatcgaa acaccggtat ttatgccggt
     9841 cggcacttac ggttcggtca aagcgatgaa cccgcaaaac ctgcatgaca tcaaagcaca
     9901 aatcatctta ggcaacactt accatctgtg gctgcgcccg ggtttggaag tcatcgaaca
     9961 attcggcggc ctgcacgaac ttatcggttg ggataagccg attttgaccg actcaggcgg
    10021 tttccaagta ttttcgttgt ccgacatgcg taagctgact gaagaaggct gtacattcca
    10081 aagcccgatc aacggcgaca aactgttcct ctcgcccgaa gtgtcgatga aaatccaaac
    10141 cgtattgaat tccgacatcg tgatgcagct ggacgaatgc accccgggcg aagcaacgca
    10201 cagccaagcg caaaaatcgc tgcaaatgag cttgcgctgg gcagaacgaa gcaaaaaggc
    10261 ttttgaggac ctcaaaaacc cgaatgcgct gtttggcatc gtacaaggcg cgatgtatga
    10321 agatttgcgc gaagaatcgc tgaaaggctt ggaagagctc gacttccctg gcttggccat
    10381 cggcggcttg tccgttggcg aacccaagcc cgaaatgtac cgtatgttgc gcgctgtcgg
    10441 ccctatcctg ccggaacaca aacctcatta tttaatgggt gtcggcacgc ctgaagattt
    10501 ggtgtacggc gtggcacacg gcgtggatat gttcgactgc gtcatgccta cccgcaatgc
    10561 gcgtaacggc tggctgttta cccgttttgg cgatttgaag atcaaaaacg ccaaacacaa
    10621 actcgacaag cgtccgattg atgaaagctg cacctgctac gcctgtcaga atttcagccg
    10681 ctcctacctg caccatctgc accgtgtcgg cgaaatcttg ggtgcacaac tcaacaccat
    10741 ccacaacctg cacttctacc aagtcatcat ggcagaaatg cgcgaagcca tagaacaagg
    10801 caaatttgcc gactggcagg cgcgattcca cgaaaaccgc gcacgcggtg tggactgact
    10861 cccggcaatg ccgtctaaag ccgcttcaga cggcattttg tcgctttacc ctacccgcac
    10921 tatttttcag aaaccgatca tgcttccatt ccttccagaa ccctcccttt cctatacgca
    10981 acaaaaccgt accgccgttt tgctgttgaa cctcggcaca cccgacgcgc cgaccgcgca
    11041 ggcggtacga ccttatctga agtcctttct gaccgaccgg cgcatcgtcg aactgcccaa
    11101 atggctgtgg tatcccattc tgcatggtct ggtactgacg ttgcgcccca aaaaaagcgc
    11161 gcacgcctat gaaaaaatct ggtttaagga aggttcgccc ctagaagttt acaccgcacg
    11221 ccaagctgcc gcgcttgccg aacgtatgcc cgacctcatc gtccgccacg ctatgaccta
    11281 cggcaatcct tcggtggcag acgtattgtc ggaactgaag gcgcaaggcg cgggcagact
    11341 gctggtcatc ccgatgtacc cgcaatatgc cgcttccagc agcggagcgg cggtagataa
    11401 agtgtgcgaa caattactgc tgcaacggaa ccaaatgagc gtccgcaccg tttcgcgctt
    11461 ttatgacgat accggctaca ttgatgcgat gaaaaaccac atcctccgat attgggcgga
    11521 acacgggcgc gggaaaaaac tgatgttgag ttttcacggc gtaccgcaga agcaccacga
    11581 cctcggcgac ccctatcccg acgagtgccg ccacaccgcc aaactgcttg ccgaagcact
    11641 ggaactgacc gaagaccaat atgtcgtatc gttccaaagc caattcggca gggcaaaatg
    11701 ggttacaccg agcacgcagg atttgttcgg caaactgccc aaacaggggg taaccgagct
    11761 ggacgtattc tgcccgggct ttttggcaga ctgtttggaa accatggaag aaatcgccct
    11821 gatggggcgc gaacagtttt acgaagcagg cggcaaaagc taccgctata tcccctgcct
    11881 caacgacaac cccgactgga tagacgcact cgtcgcactt gccgaagaaa accttggtgg
    11941 ctggcgttga aactgtttga cccatgtcta aaatgccgtc tgaacgccgt gttcagacgg
    12001 cattttcccg ctactttagc gtatgatggc atctatgccg gacaacaaca tcaagtgcaa
    12061 ccttactacc gattcggcaa gaaaatcttt cacccgctac gcaaaatcaa caacaaggtt
    12121 tcaaacatta aggaaacacc aatgaacaca acccgactgc cgaccgccct cgtcttgggc
    12181 tgcctctgcg ccgccgcttc tgccgccgac aacaatatca tgacaaaagg gcaaaaagtg
    12241 tacgaatcca actgcgtcgc ctgccacggc aaaaagggcg aaggccgcgg aaccatgttt
    12301 ccgccgctct accgctccga cttcatcatg aaaaaaccgc aggtgctgct gcacagcatg
    12361 gtcaaaggca tcaacggtac aatcaaagtc aacggcaaaa cctacaacgg attcatgccc
    12421 gccactgcca tcagcgatgc ggacattgcc gccgtcgcca cttatatcat gaacgccttt
    12481 gacaacggcg gcggaagcgt taccgaaaaa gacgtaaaac aggcaaaagg caaaaaaaac
    12541 taaacagaca aaatgccgtc tgaaaccggc aatccggctt cagacggcat tcaaatcaaa
    12601 cctttctaac gcggtacttt cagccccata gacccgatat tgccgtcccc gtcaacctcc
    12661 ttcgcggtca gcataaagcc gccgatgtca atacggccgc cctcgaccgg ctcctgacta
    12721 tcggaacgct tatcgaaaag ctcggcaagg ctcaaaccct cttcgcccgc ttccagcctc
    12781 aaaccgtaag taagtgccaa atcacccgaa cgtgctgccg gcgaaacgac aaactcgccg
    12841 aagaaatcaa aatgctcacg gatgccgata tccgtctcgg taaagtattt cgccatttta
    12901 tagtgaa
//
LOCUS       DAXIEB010000056        12864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000056 DAXIEB010000000
VERSION     DAXIEB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.056"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            176..1627
                     /gene="cysG"
                     /locus_tag="ACFOA3_001254"
     CDS             176..1627
                     /gene="cysG"
                     /locus_tag="ACFOA3_001254"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6898260.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNTELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLREKLEALLPPSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            1643..2377
                     /locus_tag="ACFOA3_001255"
     CDS             1643..2377
                     /locus_tag="ACFOA3_001255"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6898261.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            2415..3338
                     /gene="cysD"
                     /locus_tag="ACFOA3_001256"
     CDS             2415..3338
                     /gene="cysD"
                     /locus_tag="ACFOA3_001256"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6898262.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVTDISERSATRLDDQASEAAMEKRKKEGYF"
     gene            3377..4663
                     /locus_tag="ACFOA3_001257"
     CDS             3377..4663
                     /locus_tag="ACFOA3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6898263.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVSAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            4660..6474
                     /locus_tag="ACFOA3_001258"
     CDS             4660..6474
                     /locus_tag="ACFOA3_001258"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6898264.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSVLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDQATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            6501..8270
                     /gene="cysI"
                     /locus_tag="ACFOA3_001259"
     CDS             6501..8270
                     /gene="cysI"
                     /locus_tag="ACFOA3_001259"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6898265.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFSIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            8443..10302
                     /gene="ilvD"
                     /locus_tag="ACFOA3_001260"
     CDS             8443..10302
                     /gene="ilvD"
                     /locus_tag="ACFOA3_001260"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6898266.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAGEIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(10365..10598)
                     /locus_tag="ACFOA3_001261"
     CDS             complement(10365..10598)
                     /locus_tag="ACFOA3_001261"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6898267.1"
                     /translation="MQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            10585..10824
                     /locus_tag="ACFOA3_001262"
     CDS             10585..10824
                     /locus_tag="ACFOA3_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898268.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            10948..11514
                     /locus_tag="ACFOA3_001263"
     CDS             10948..11514
                     /locus_tag="ACFOA3_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6898269.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQRPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKINPEKPLSGGLFI"
     gene            complement(11660..12712)
                     /gene="bioB"
                     /locus_tag="ACFOA3_001264"
     CDS             complement(11660..12712)
                     /gene="bioB"
                     /locus_tag="ACFOA3_001264"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6898270.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
BASE COUNT         3086 a         3778 c         3509 g         2491 t
ORIGIN      
        1 acggaaaacc gccgcatccg cagccgccct catatagctt tcagcttcta acaaatgcct
       61 ttttattatt taaccgggaa aatccttttg tctaaaatga cccgcattcc gacaaaaatg
      121 ccacgacgca ccgtatcgga aacaaggtgc gggcaaacag caaaggagtc agccagtgaa
      181 ttatttcccc attttcgcca acctcgccgg ccgccccgta ttggtcgtag gcggcggcgc
      241 ggtcgccgca cgcaaaatca gcctgctgct gaaggcgggc gcagaggtca gggttgccgc
      301 aaaacacctg aataccgaac tctccgcgtt ggcggcggaa aacaaaatcc tgtggcttgc
      361 cgaagaattt cgtgccgaac acatccgcac ggttttcctc atcattgcgg caagcagcga
      421 ccaagccctc aaccggcgcg ttttccatct tgccgaaagc tgccaaaagc cggtcaacgt
      481 ggtggacgac cgcgaccatt gcagcttcat cttcccatcg gttatcgacc gcaacccggt
      541 tcaaattgcc gtttccagct ccggcagcgc gcccgtcctc gcccgtttgc tgcgtgaaaa
      601 actggaagcc ctgctgccgc cgtctttggg cgatatggcg gaaatctccg gaagatggcg
      661 cgatgcggtc aaagccaaac tgaaatccgt taccgaacgc cgccgttttt gggaaaagca
      721 gttcaacggc cgttttgccg ccctcgttaa aaatcaacaa accgcccaag ccgaacagga
      781 gttggcaaag cagttggaac aaaattatca aggcggtttt gtgtctttgg tcggtgcggg
      841 tcccggcgat gcggggctgc tgacgctcaa agggctgcag gaaatccagc aggcagacgt
      901 ggtgctttac gacgcgctgg tttcagacgg catactgtcc ctcgtccgcc gcgatgcgga
      961 acggattttt gtcggcaagc gcgcccgggg cggccgcacc ccgcaagagg acaccaatgc
     1021 gcttatggtc aggctggccc gcgaaggcag gcgcgtggtg cgtttaaaag gcggagaccc
     1081 tttcgtgttc ggacgcggcg gcgaagaatt ggaaaccttg gcacgccatc agattccgtt
     1141 ttcggtcgtc cccggcatta ccgccgccgt cggcgcgacc gcctatgccg gcatcccgct
     1201 cacgcaccgc gattatgccc aaagtgcggt tttcgtaacc ggccaccgca aggcggatgc
     1261 gcccgacatc gaatggcaga ccctcgcccg cagccgccag accttggtga tttatatggg
     1321 tgcgctcaaa gccgccctga ttgccgaacg gctgcaacag cacggacgct cgcccgacac
     1381 gccggcagcc gttatcagcc aaggcacgct gccggctcaa aaaaccgcaa ccggcacgct
     1441 cgccaacctt gccgaactcg ccgaaaccgc gccgaatccg gcattgattg tcataggcga
     1501 agtggtgggg ctgcacgaaa aactggcttg gttcggcgaa aacgccaaaa aagaaagcaa
     1561 tccggccgaa cacgcgtatt ttgcattgga cggactgggc accggacaag aacaacaagc
     1621 cgcataaagc aaggcggaca aaatgaaaat tttcaaacct gctttatgga aaattccgcc
     1681 gattgaaaat ggcagtgaaa cggctttggc ggaaaaaacg gaaaccctca aacaacgcct
     1741 gcaccggatt gccggcagcc accgggatgc acggtttgcc agcagccttg ctgcggaaga
     1801 tatggtaatt accgacctta tcgccggaga aaacctcaat atcggtattt ttactttgga
     1861 tacggggctg ctgcacgcgg aaactttaaa cctgttggat aggattgaac gtgtttatcc
     1921 gcatatgcaa atcaagcgtt tccaaccgat acgggaagat gccctgcatt atgtggaaag
     1981 caaaggcagg tttgcctttt acgacagcgt tgaagcgcgc cgcgaatgct gccgcatccg
     2041 aaaaaccgaa ccgctcgacc gcgccattgc cggggcggat gcttggctta ccgggcaacg
     2101 ccgcgaacag tccgccacac gcacggaact gccgtttgcc gaatatgatg ccgggcgcgg
     2161 catcgataaa tacaacccga ttttcgactg gtcggaacac gacgtgtggg catacatcct
     2221 tgccaacaat gtgccttaca acgatttgta ccggcaaggc tttcccagta taggctgcga
     2281 cccctgcacc cgccccgtca aggccggcga agacatccgc gccgggcggt ggtggtggga
     2341 agataaaaac agcaaagaat gcgggctgca caaataattc ataaggatca aacagaagaa
     2401 ataaggaata tcgtatgacg aaaaccgaac cgaacaacgt ccaactcgac tggttggaat
     2461 ccgaatccat ccacattatc cgcgaagtgg cggcagaatg cgaaaacccc gccctgctct
     2521 tttccggcgg caaagattcc gtggtcctgc tcgccctcgc ctgcaaagcc ttccggctgg
     2581 gcagcaggcc ggtaaaactg cccttcccgc tggtgcatat cgacacgggg cacaactacc
     2641 ccgaagtgat tgccttccgc gacgcgcagg cggcaaaact caatgcccgc ctgatagtgg
     2701 ggcgcgtgga agactccatc gctaaaggca cagtagtttt acgcaaagaa accgattcgc
     2761 gcaatgcggc gcaggcggtt acgctgttgg aaaccatcga ggcaaacggc tttgacgcgc
     2821 tgatgggcgg cgcgcggcgc gacgaagaaa aagcccgcgc caaagaacgg attttctcgt
     2881 tccgcgacga gttcggacaa tgggatccga aggcgcaacg ccccgagctg tggtcgctgt
     2941 acaacaccag gctgcacaaa ggcgaaaata tgcgcgtctt cccgatttcc aactggacgg
     3001 aactcgacat ctggcaatac atcgcccgcg aaaacctcga gctgccgccg atttattaca
     3061 gccacaggcg cgaagtggtc agacgcaggg ggctgctggt ccccgttacg ccgcttacgc
     3121 cgaaaatgcc gtctgaaacc tccgaaatcc ttgatgtccg cttccgcacc gtcggcgaca
     3181 tcagctgcac ctgcccggta gaaagcaccg cgtccacgcc gacggagatt atcagggaaa
     3241 cggcagttac cgacatttcc gaacgcagcg cgacccggct ggacgatcag gcaagcgagg
     3301 cggcaatgga aaaacgcaaa aaagaaggct atttctaaat ttcaaaaacc ctttaagcag
     3361 atatgaggaa gaaaatatga cggcacaaca ccaggccccg ctccgcttca ttaccgccgg
     3421 cagcgtcgat gacggcaaaa gcaccctgat cggacggctg ctctacgaca gcaaagccct
     3481 gctgtccgac caaatcaaaa ccttggaatc cggcaaaagc aaaggtttga aagaagccat
     3541 agacttctcc atcctgaccg acggactgga agccgaacgc gaacagggca ttacgatcga
     3601 tgtggcatac cgctattttt ccacggcgaa acggaaattc atcatcgccg acacgccggg
     3661 gcacgaacaa tacacgcgca atatggttac gggcgcaagc accgcttcgg cggcagtcgt
     3721 gctggttgac gcatcccaac tggatttcgg cgcgcagccc ttgcagcttc tgccgcagac
     3781 caaacgccat tccgccatcc tccgccaact caactgcccg catattgtgg tggcggtcaa
     3841 caaaatggac ctgctggatt acagcgaaga caaattcaac gccattgtgg aggcataccg
     3901 ccgacttgcc gaacagctcg gtttgaaaga cgcgcatttc gtaccggtgt ccgcactctt
     3961 gggcgacaac attgtttacc ccggcggcaa tatgccttgg tataaaggcg aacccttatt
     4021 gtccattttg gaaaccctgc cgggggcgga cgaagtcagc cgcaccgccg acgattttta
     4081 tttccccgtg caactggtcg tccgccaaga tgccgacaag gccgatgatt tccgaggcta
     4141 tcaggggcgc atcgaacgcg gttcggtcgc cgtcgggcaa accgtccgca tcgaaccgaa
     4201 cgggctgacc gccgaagtgt ccgaaatcat cacgccgaaa ggggaagtgg cgcaggcttt
     4261 tgccggcgag gcggcaaccc tccggctgga tcgcgacatc gatgtttcgc gcggcgacct
     4321 ttttgtcgat aaaaattccc cgctcgcccc gcaaaaacat ctggaagcca cgctttgctg
     4381 gtttgacgaa cgtccgctca acaccgcgcg caaatacctg ctcaagcacg gcacgcaaac
     4441 cgtgtcggca aaagtcgggg aaattgaaag cgttttggat gtccgcacgc tggaacaaga
     4501 ggccggcgcg gaatccttga agatgaacga catcgccaaa gtccgcatca accttcaaaa
     4561 acccgttacg gcaacgcctt atgcggaaaa cactgccgcc ggttcgttta tcctgataga
     4621 cgaagcgaca tacggcactg ttgcggcagg tatgatttta tgagtgaaca cgatatgcag
     4681 aacacaaatc cgccattacc gcctctgccg cccgaaatca cgcagctcct gtcggtgctg
     4741 gacgcggcac aatgggcgtg gctgtccggc tacgcttggg caaaagcagg aaacggggca
     4801 tctgcaggac tgcccgcgct tcagacggca ttgccggcgg cagaaccttt ttccgtaacc
     4861 gtcctttccg cctcgcaaac cggcaatgcg aaatccgttg ccgacaaagc ggcggacagc
     4921 ctggaagccg ccggcatcca agtcagtcgc gccgaactga aagactataa ggcgaaaaac
     4981 atcgccggcg aacgccgcct gctgctggtt acctccaccc aaggcgaagg cgaaccgccg
     5041 gaagaagccg tcgtgctgca caaactgctg aacggcaaaa aagccccgaa attggacaaa
     5101 ctccaatttg ccgtactggg cttgggcgac agctcctatc cgaatttctg tcaggcaggt
     5161 aaagatttcg accggcgttt tgaagaattg ggggcgaaac gcctgctcga acgcgttgat
     5221 gcggatttgg actttaccgc ctccgcagac ggatggacag gccgcattgt cgcgcgctta
     5281 aaagaagaag ccgcaaaaaa ccgggcaacg cccgcgccgc agacaacgcc ccccgccggc
     5341 cttcagacgg caccggatgg caggtactgc aaggcagacc cctttcccgc cgccctgctg
     5401 gccaatcaga aaatcaccgc ccgccaatcc gataaagacg tgcgccacat cgaaatcgat
     5461 ttgagcggtt cggatttgca ctacctcccg ggcgacgcgc tcggcgtttg gtttgacaac
     5521 gatccggcac tggtcaggga aatcctagac ctgctcggca tcgatcaggc aacggaaata
     5581 caggcgggcg gaaaaaccct gccggttgcc tccgcactgt tatcccattt tgaactcacg
     5641 caaaacaccc ccgcctttgt caaaggctat gccccgttcg ccgatgatga cgaactcgac
     5701 cgtattgctg ccgacaacgc cgttttgcaa ggctttgtgc aaagcacgcc gattgccgat
     5761 gtgctgcacc gcttcccggc aaaactgacg gcggaacaat tcgccggcct actgcgcccg
     5821 cttgcgccgc gcctgtattc gatttcctcg tcgcaggcgg aagtggggga cgaagtgcac
     5881 ctgaccgtcg gcgcggtgcg tttcgaacac gaagggcgcg ccagggcggg cggcgcatcg
     5941 ggtttccttg ccgaccggct ggaagaggac ggcacggtgc gcgtgtttgt ggaacgcaac
     6001 gacggcttca ggctgcccga agacagccgc aagccgattg tgatgatcgg ctcgggcacc
     6061 ggcgtcgcac cgttccgcgc tttcgtccaa caacgtgccg cagaaaatgc ggaaggcaaa
     6121 aactggctgt ttttcggcaa tccgcatttt gcccgtgatt ttctctatca aaccgaatgg
     6181 cagcagtttg ccaaagacgg cttcctgcac agatacgatt tcgcctggtc gcgcgatcag
     6241 gaagaaaaaa tctatgtgca ggacaaaatc cgcgaacagg cggaaggact ttggcaatgg
     6301 ctgcaggaag gcgcgcatat ctatgtgtgc ggcgatgcgg caaaaatggc aaaagacgtg
     6361 gaggccgcct tgctggatgt gattatcggg gcaggacatt tggacgaaga gggcgcagaa
     6421 gaatatttgg atatgctgcg cgaagaaaaa cgctatcagc gtgatgttta ttgattaaat
     6481 ataatcggga ggaacacaaa atgaccgtac agaccaagac aaaaggtttg gcgtggcaag
     6541 aaaaaccgct atccgacaac gaacgtctga aaaccgaaag caatttttta cgcggcacga
     6601 ttttggacga tttgaaagac ccgctcacgg gcggcttcaa aggcgacaac ttccaactca
     6661 tccgcttcca cggtatgtat gagcaggacg accgcgacat ccgcgccgaa cgcgccgagg
     6721 caaaactcga gcccttgaaa tttatgcttt tgcgctgccg gctgccgggc gggatcatca
     6781 aaccgtccca atggatagaa ctggacaaat ttgcccggga aaacagtcat taccgctcca
     6841 tccggctgac caaccggcaa accttccaat ttcacggcgt gccgaaagcc aagttgcaga
     6901 cgatgcaccg cctcttgcac aaactgggtt tggattccat cgccacggcg gcggatatga
     6961 accgcaacgt gctttgcacg tccaacccga tcgagtccga actgcaccgg caggcttacg
     7021 aatacgcgaa aaagatttcc gaacacttgc tgccgcgcac gcgcggttat ctggatgtgt
     7081 gggtggacgg caaaaaagtt caaagttctg acgacttcct tcaagaagac gagccgattt
     7141 tgggcaaaac ctatctgccg cgcaaattca aaaccgcagt cgtcatcccg cccttaaatg
     7201 atgtggactg ctacggcaac gacttggatt ttgtcgccat ttcagacggc aacggacagc
     7261 ttgccggatt caatgttttg gcgggcggcg ggctttcgat ggaacacggc aacaccaaaa
     7321 cctatccgaa catttcactg gaactgggtt tcgtgcctcc ggaacacgcg ctgaaggccg
     7381 ccgaagcggt ggtaaccacg cagcgcgact tcggcaaccg cagcgaccgc aaaaacgccc
     7441 gcacccgcta caccattcaa aatatgggct tggacaactt ccgcgccgaa gtcgaacgcc
     7501 gtatgggtat gccgttcgaa cccgtacgcc cgttcaaatt taccgggcgc ggcgaccgta
     7561 tcggctgggt gaaaggcata gacggcaact ggcatttgac ccttttcatc gaaagcgggc
     7621 gtttggttga cgaaggcggg aaacagcttc tgaccggcgt gttggaaatc gccaaaatcc
     7681 acaaaggcga cttccgcatc accgccaacc aaaacctcat cgtggcaaat gtacccgaag
     7741 gaggcaaagc ccggatcgaa cagctcgccc gctcatacgg gctgatacac gacggtgtca
     7801 gcaaactgcg cgaaaatgcg atgtcgtgcg tttccttccc gacctgcccg ctggcaatgg
     7861 cggaagccga acgcgtgctg ccggacttca tcggcgagct ggataagatt atggcgaaac
     7921 acggcacgtc ggacgactac atcgttaccc gcattaccgg ctgcccgaac ggctgcggac
     7981 gggcgatgtt ggcggaaatc ggactggtcg gcaaagccgt cggacgctac aacctccata
     8041 tcggcggcga ccgtgaaggc gtacgcatcc cccgtcttta caaagaaaac atcaccctgc
     8101 ccgaaatcct tgccgaattg gacgacctga tcggcaaatg ggcggcagaa cgcaatatcg
     8161 gcgaaggttt cggcgatttc tccatacgga cgggcatcgt caaacccgta ttaaatgcac
     8221 ccgttgattt ttgggacgca tccaaagccg tcgcgattgc ccgcgcctga acccgtcccc
     8281 gcattcctgc ggggattttc ttttttgccg cacgccgttt caatcccgca aaattccgat
     8341 acttggtata aaataccctc ttttcccact ctaaaaaacc gtacccgata ccgtctgaaa
     8401 cagccctgtc tttcagacgg tataggcaca aggaaacaca ctatgccaga ataccgctcc
     8461 aaaacctcca cccacggtcg caatatggcg ggcgcgcgcg cgttgtggcg cgccaccggc
     8521 gtgatggaaa ccgacttcgg caaaccgatt atcgccgttg ccaactcgtt cacccaattc
     8581 gtgccaggtc atgtccacct gcacaatatg ggccagctgg ttgccggaga aatcgaaaaa
     8641 gccggcgcca tcgccaaaga attcaacacc atcgccatcg acgacggcat cgctatggga
     8701 cacagcggta tgctgtattc cctgcccagc cgcgatttga ttgccgactc catcgaatat
     8761 atggtcaatg cccactgcgc cgacgcgctg gtgtgcattt ccaactgcga caaaatcacc
     8821 ccgggcatgc tgattgccgc gatgcgcctg aacatcccca ccatcttcgt ctccggcggc
     8881 ccgatggaag caggtaaagt tatcggcgtg gcaaacatcc aaccagaacg ccgcttggac
     8941 ttgattgacg cgatgatcga atcggcagac gacaatgtca gcaaccggca agtcgaagaa
     9001 gtcgaacaaa acgcctgccc gacctgcggc tcgtgttcgg gtatgtttac ggcaaactcg
     9061 atgaactgcc tgaccgaagc actcgggctg tccctgccgg gcaacggttc ttacctcgcc
     9121 acccacgccg gccgcaaaga actgttcctc gaagccggcc gtatgattgt cgaaatcacc
     9181 aaacgctatt acgaacaaaa cgacgaaacc gtgctgccgc gcagcattgc caccaaaaaa
     9241 gcgtttgaaa acgccatgac gatggatatt gcgatgggcg gcagcaccaa taccattttg
     9301 cacctgttgg ccgttgccaa cgaagccggt gtcgatttca aaatggccga cattgaccgc
     9361 ttaagccgcg ttgtgccctg catctgcaaa accgcaccca acaaccacga ctactatatg
     9421 gaagacgtgc accgcgccgg cggcattttc gccatcctga aagaattgga caaagcgggc
     9481 aagctgcaca ccgacgtgca cactatccac gcaccgacgc tgaaagacgc gattgaaaaa
     9541 tgggacataa ccaaccctga aaacacccgc gccatcgaac gcttcaaagc cgcgcccggc
     9601 ggcgtacgca ccacccaagc gttctcgcaa aaccgtatgt ggaaaaccct cgacctcgac
     9661 cgcgaaaaag gctgtatccg cgacgtggca cacgcctact cgcaagacgg cggtttggcg
     9721 gtcttgttcg gcaacatcgc cgagcgcggc tgcgtggtaa aaaccgcagg cgtggacgag
     9781 agcatcctca aattcaccgg ccgcgcccgc gtgtttgaaa gccaagaaga cgcagtagaa
     9841 ggcattttgg gcaaccaaat cgtcgctggc gacatcgtca tcatccgcta cgaaggcccg
     9901 aaaggcggcc cgggcatgca ggaaatgctg tatccgactt cctacctgaa atctaaaggc
     9961 ctcggcaaag cctgcgctct cttaaccgac ggacgcttct ccggcggcac atcaggtttg
    10021 tccatcggac acgcctcgcc cgaagcggcg gaaggtggcg cgatcggttt ggtacacgaa
    10081 ggcgataccg tcgaaatcga catccccaac cgcagcatcc accttgccat ttccgatgaa
    10141 gagcttgccg cacgccgtgc cgaaatggaa gcgcgcggca gcaaagcatg gaagcctaaa
    10201 aaccgcgacc gctacgtctc cgccgcatta agagcttacg gcgcgatggc gacttccgcc
    10261 gacaaaggcg cggtgcgcga cgtagcgcaa atcgaaagat aagccccaaa acgtgcggca
    10321 aatgctgtct cgtctgaaca gtaaaggtct gatgatggac aaatttatct gggcgatggc
    10381 ggtattttcc gcaattttgg cctcgttatc ggcagcggct ttagcgcgat cgagtatgta
    10441 aaagagctgc cgctcgccgc cgttgccgtg atgccttgcg cgccgaagtc gttgctgtgg
    10501 ttgttggcga agctgaggta gtcgaatccc gcgtcggcaa ggtattgccc gtatgcggag
    10561 ggcgttcgga atgcatagca tattttgggg ggttgcacat tttttcggcg taccgccttc
    10621 gtcaaacagc gtgccttcga ggttgccgac ggtaatgtcc gcgtcttgca aggcagattc
    10681 gacgtttttc agaatattgg tatcgggcag gtaatcgacc ggataattgc tgccgagcat
    10741 aatgtcgccc acgccgataa tggaaacggt atcggctgtc ttttcgtcag gcgcatcctg
    10801 tccgtccaca tcgccggtat ttgaaacggg cgcggggttt gaaccgtcaa tcggcagcac
    10861 ggggattccg ttccggcagg atgcgccgag ggattgaccc cggcggtttg cggtgcggaa
    10921 acggcataaa gggcgaacct tgacgaattg aacatcaaat attcgggcat cgaaaacagg
    10981 tttgaaaccg ccatcctgaa gaaaaacggc gtaagatccg gcttcgtctc cttcgccccc
    11041 aaccttgccg ccgtcaaact gaacgattat gccaaagtta gaaaacggat taccaaaacc
    11101 aaacagaaag ccgacatcgt cattgtgatg ttccacggcg gcgcggaagg gaaacaggcg
    11161 gaacacctgc cgttcgatac cgaaatcttc tatggggaaa acaggggcaa cgtcgttgag
    11221 tttgcgcggc ttgccgtcga ttccggcgcg gatgtcgtat tcgggcagag gccgcacgtt
    11281 actcgcgccg tcgaacttta ccgcgaccgc ttcatctcct acagcggcgg caactttgcc
    11341 acctacggcg caatcaacac ctccggcatc agcggcatcg cccccatttt taaaattatc
    11401 accgacaaac agggtaaatt tatttccgga accatcatcc cgattaccca agccgacgat
    11461 aaaatcccca aaatcaaccc tgaaaaaccg ttatcgggcg gattatttat ctgaacagca
    11521 gcgacttccc caacgggaac ggactggatg tctcgcccga cggcagcatc acgcgccggt
    11581 aaaaagatat tggaaatgcc gtccggacaa atgccgtctg aaagcctttt tccgggtttc
    11641 agacggcatt tttcaatcgt caataatcca ctttaatccc cgaggctttt tccacttcgg
    11701 cgacctcgcc ttccagctca aactgtaagg gatacaggtt aagcttttcc atcaggattc
    11761 tgtcgccgtc ttcgtccgga ttgcctgtgg tcaacagctt gtcgccgtaa aaaatcgaat
    11821 tcgcgcccgc cataaagcac atcgcctgca tcgcttccgg catattgctg cgccctgccg
    11881 acagccggac ataactttgc ggcatcgtaa tccgcgccac ggcgatggtg cgaacaaatt
    11941 ccgtccagtc caaatcttcg gcatcgtcaa gcggcgtgcc ttccactttg accaaccggt
    12001 taatcggcac gctttcgggc tgcgggtcga ggttggcgag gctggcaatc agcccggcgc
    12061 gttcggcacg agtttcgttc atcccgacga tgccgccgca gcagactttc aaaccggcgt
    12121 tgcggacttt gcccaaggtg tccatacggt cttcatgccg gcgtgtatgg atgatgtcgt
    12181 tgtagcggtc ggggtcggtg tcgaggttgt ggttgtaata atccaagccc gcctctttca
    12241 agtcttccgc cataccttct tcgagcatac cgaacgtgcc gcaggtttcc atacccaagc
    12301 ccttgacggc tttgatgatt gcggaaaccg tctccacgtc tttgggtttg gggccgcgcc
    12361 atgccgcgcc catacaaaac cggcttgcgc cgcgcgattt ggcgattttg gctttttcga
    12421 cgatttcatc cacatccatc atctgctctt tgcccagatt ggtgttgtgg tgcgccgatt
    12481 gcggacagta ggcgcaatct tcgggacaac cgccggtttt gatggacaac agcgtggaaa
    12541 gctggatttc gcgcgggttg aaattttggc ggtggatttc ggcggcttgg taaatgaggt
    12601 cgaggaaggg aagtccgaac agggcttcga catcgcattt tttccaatag cgcgcggtgg
    12661 gatgcggctt gcactcggtc ttgcggcgca aggcgacggg ggatacggtc ataatgtgtt
    12721 ctttcgtatt tacagcggcg caatgacgct gacgtgcaga ccgccggcgc aatgacggcg
    12781 gttgcgggta atcaaatggc tgaaaacagc ggcggcaagt gtaacgcgtt tgaaaatacg
    12841 gggcaaacgg tttttccgcc tttg
//
LOCUS       DAXIEB010000057        12455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000057 DAXIEB010000000
VERSION     DAXIEB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.057"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..554)
                     /locus_tag="ACFOA3_001265"
     CDS             complement(<1..554)
                     /locus_tag="ACFOA3_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HFC6898271.1"
                     /translation="MEDKQGMTKAVVGVMTDAPADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGLYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFA"
     gene            657..967
                     /locus_tag="ACFOA3_001266"
                     /pseudo
     CDS             657..967
                     /locus_tag="ACFOA3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1024..2082)
                     /gene="trpD"
                     /locus_tag="ACFOA3_001267"
     CDS             complement(1024..2082)
                     /gene="trpD"
                     /locus_tag="ACFOA3_001267"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6898272.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(2146..2736)
                     /locus_tag="ACFOA3_001268"
     CDS             complement(2146..2736)
                     /locus_tag="ACFOA3_001268"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6898273.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3156..5432
                     /gene="znuD"
                     /locus_tag="ACFOA3_001269"
                     /gene_synonym="tdfJ"
     CDS             3156..5432
                     /gene="znuD"
                     /locus_tag="ACFOA3_001269"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6898274.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(6410..7195)
                     /locus_tag="ACFOA3_001270"
     CDS             complement(6410..7195)
                     /locus_tag="ACFOA3_001270"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6898275.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            7350..10196
                     /gene="uvrA"
                     /locus_tag="ACFOA3_001271"
     CDS             7350..10196
                     /gene="uvrA"
                     /locus_tag="ACFOA3_001271"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6898276.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEGTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYSFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(10228..10398)
                     /locus_tag="ACFOA3_001272"
     CDS             complement(10228..10398)
                     /locus_tag="ACFOA3_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898277.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(10413..10598)
                     /locus_tag="ACFOA3_001273"
     CDS             complement(10413..10598)
                     /locus_tag="ACFOA3_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898278.1"
                     /translation="MTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKAPPTQ
                     NPGSSWIQIKRTPDSSR"
     gene            complement(10614..10883)
                     /locus_tag="ACFOA3_001274"
     CDS             complement(10614..10883)
                     /locus_tag="ACFOA3_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898279.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            11039..11962
                     /locus_tag="ACFOA3_001275"
     CDS             11039..11962
                     /locus_tag="ACFOA3_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFC6898280.1"
                     /translation="MKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKINRKDLMQ
                     AFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIKILNGSL
                     IFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGLSEKVIV
                     PLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDIDWNRIV
                     KIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDHHIDDPA
                     FRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(12189..>12455)
                     /locus_tag="ACFOA3_001276"
     CDS             complement(12189..>12455)
                     /locus_tag="ACFOA3_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898281.1"
                     /translation="LKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
BASE COUNT         3236 a         3366 c         2798 g         3055 t
ORIGIN      
        1 aatgcgaaaa gtcgtgattc ccagccttct tttgcgacgc cgcaaccggt gccggtcagt
       61 tcgaaaagga tggtattgtt ttgtccgcca aaatggacgc gaccgtatag gacgtcttcc
      121 gaacccatca accaacagcg ctcataaaaa cgaccgcccg agcctttgga ctctttgtag
      181 agaccgaaac cgaaaacttc ttcggcgagc atggacgcgg cgcgaataaa atcttcgtct
      241 tccaaaagac ttacacgaac gccgtattta tcgaaaaagg ttttttcatg aaatgaaaag
      301 ctaatttgat caatgaaagc cgaatctgat acaccgcgcc gaagaggaac gcctaacagg
      361 tttcctttac cgtccgttat gtacgtttcg taacattcga agacttcctg aaccctgcct
      421 gccgtttcgg tttctgtacc ccccctgtta gataaggggg gaaggtttga agcggttgcc
      481 ggcttcctgc cgtccgccgg tgcgtccgtc atcacgccga caaccgcctt tgtcatccct
      541 tgcttatctt ccatggtgcg aatcctcaaa aacgggcaaa aaaaaagccc tgttacttgt
      601 agaaagtaaa ggacgttaat ttttgttaat cgtcccttct tagggacgca atatataagg
      661 tttataccgt cgttgttcct aatgcgcaat cagcgacatt gttgccaatt atccgaaaga
      721 aagttaagcc tgatggcatt gtatacacgg atacctttcg tagttatgat gtgcttgata
      781 ttagtgaatt tagccattta cgtaagttta acggcattcc caaagagcat ttggggctgc
      841 atttaaagaa atgccaatgg cattttaaat agaatctcaa attcagattt taagacaatt
      901 agttaatggg aatttggtct ggttatctag tacagcccca agtttttata taaaaacagt
      961 ttggtaagtt ctcttcgtga agtgcttatg ttttgccaca aaagttccat aatcatggcg
     1021 gcatcaccca agctccatct ttccaagaaa atggcatctt tgttgtggtt gaaaaccgac
     1081 aaactcctct tttttcgatt tggccctgcc tgaatcgatg gcttcccgtg cggcagatat
     1141 gccgtctgaa agcgaagcag cgacatttcc ggcatacagg gcggcggcgg tgttgagcaa
     1201 tacgatatcg cgcgcagccc cttctcttcc ttccagcacc tcattcattt tcaacaaaga
     1261 ttcctgagta ttggcaactt tgatttcatc caaattgcgg cgggtttcga taccgaaatc
     1321 ttctgggcgg atgtcgtatt cgctgatttt tccgtctttg agctcggcaa cgcgtgtttt
     1381 gcccgtcagt gtaatttcat ccaaaccgcc ctccccgcaa acaaccaaaa catgcttgga
     1441 gccgagctgt tgcaatactc tggaaaggat gccgcacaaa tcggtgtgga acacgcccaa
     1501 aagctggttc ggcgcgcccg caggattggt caacggaccc aatatgttga aaatgcttcg
     1561 gaaaccgagc gaacggcgta ccggggcgac atggcgcatg gcactgtggt gattgggggc
     1621 gaacataaac cctataccgg tctggctgat actttgggca atctgttcgg gagtcaggtt
     1681 gaggtttgcg cccatctgct ccatcacgtc cgccgcgccg ctggaagatg aaacggaacg
     1741 cccgccgtgt ttggcaacct tcgcgcctgc cgctgcggca acaaacatcg aagtcgtcga
     1801 aatattgaag gttttggcac catccccgcc cgtaccgacg atatcaacca gcccctccgc
     1861 attctccagc ggcacttttg tcgcaaactc gcgcatgacg gctgcagctg cggtaatttc
     1921 ggaaacggtt tcaaccttga tacgcaatcc tgtcaaaatc gccgcaattt gttcgggcgg
     1981 cacttttccg ctcatcattt ggcgcatcaa gtcggtcatt tcatcgtaaa acaactcatt
     2041 attgctgatt aatcgttcga tagcctgttg cggtgtaatc attttttgtc ctccgttcaa
     2101 tattcggacg aaaatgccgt ctgaagggct tcagacggca tcacgtcaga ttttttgcgg
     2161 tttgaagttt tgaaattcga ttaaaaaatt gtttaacata tcatgtccgt gctcggtcaa
     2221 gagggcttcg gggtggaact gcacgccctc gacggcatat tccttatggc gcacacccat
     2281 aatctcgccg tcctcagtcc aagccgttac ttccaaacat tcgggcatcg tattccgatc
     2341 gataacgagg ctgtgataac gcgtacaggt aaccggattg ggcaaaccct taaacatacc
     2401 cttgcccgaa tgggacacgg gcgacacctt accgtgcatc agcgttttgg cgcggactat
     2461 cctgccgccg aacgcctcgc ctatcgtctg atgcccgagg cacacgccca taatcggcag
     2521 ccggccggca aaatggcgca tcgccgccac ggaaatcccc gcttctttgg gcgaacacgg
     2581 gccgggtccg ataacgagat attgcggatt caatgcctcg atttcttcca acgtaatatc
     2641 atcgttgcgg cgcacggcga cttcctgccc caattcagcg aaatactgga cgatgttgta
     2701 agtaaaactg tcgtaattgt cgataaacaa aagcattttg tgattaacct attgatttat
     2761 aatttgtttg ttattaactg catttccgat accaccatcc atacccctta tatcttagcg
     2821 tgcccgatgc gccctcgtga acctgggcag agcctcgcgt tctgacaagt acgccatttt
     2881 gccacctttt cgaccgttcg ggaacgtgcc gtttttatgc actcataaat tttggttatc
     2941 ccgcagccga acatagtggg caaacacagc ggaatctgcg tcaacatcaa ccgcgccccc
     3001 gaatgacggc ggatacaaat ggacaccgcc cgatgccgca accgtttcat ttgaaaaact
     3061 tttgcctatg ccctctgagt gttctcaaaa tcttatgata taaaaaatgt aatgttatat
     3121 aataacaaac ttttgaaatg cgacgagaag ccaaaatggc acaaactaca ctcaaaccca
     3181 ttgttttatc aattctttta atcagcaccc ccatcctcgc ccaagcgcat gaaaccgagc
     3241 agtctgttga cttggacgag gttaccgtcg tcggcaaaag ccgtccgcgc gccacatccg
     3301 ggctgttgca cacttcgacc gcttccgaca aaatcctctc cggcgatacc ttgcgccaaa
     3361 aagccgtcaa cttgggcgat gctttggacg gcgtgccggg cattcacgcc tcgcaatacg
     3421 gcggcggcgc gtccgctccc gttattcgcg gtcaaacagg caggcggatt aaagtgttga
     3481 accatcacgg cgaaacgggc gatatggcgg atttttcgcc cgatcacgcc attatggtag
     3541 ataccgcctt gtcgcaacag gtcgaaatcc tgcgcgggcc ggttacgctc ttgtacagct
     3601 cgggcaatgt ggcggggctg gtcgatgttg ccgatggcaa aatccccgaa aaaatgcctg
     3661 aaaacggcgt atcgggcgaa ctcggattgc gtttgagcag cggcaatctg gaaaaactca
     3721 cgtccggcgg catcaatatc ggtttgggca aaaactttgt attgcacacg gaagggctgt
     3781 accgcaaatc gggggattac gccgtaccgc gttaccgcaa tctgaaacgc ctgcccgaca
     3841 gccacgccga ttcgcaaacg ggcagcatcg ggctgtcttg ggttggcgaa aaaggtttta
     3901 tcggcgtagc gtacagcgac cgtcgcgacc aatatggtct gcctgcccac agccacgaat
     3961 acgatgattg ccacgccgac atcatctggc aaaagagctt gattaacaaa cgctatttac
     4021 agctttatcc gcacctgttg accgaagaag acatcgatta cgacaatccg ggcttgagct
     4081 gcggcttcca cgacgacgat gatgcacacg cacacaccca cagcggcaga ccgtggatag
     4141 acctgcgcaa caaacgctac gaactccgtg ccgaatggaa gcaaccgttc cccggttttg
     4201 aagccctgcg cgtacacctg aaccgcaacg actaccgcca cgacgaaaaa gcaggcgatg
     4261 cagtcgaaaa cttttttaac aaccaaacgc aaaacgcccg catcgagttg cgccaccaac
     4321 ccataggtcg tctgaaaggc agctggggcg tgcaatattt acaacaaaaa tccagtgctt
     4381 tatctgccat atccgaagcg gttaaacaac cgatgctgct tgacaacaaa gtgcaacatt
     4441 acagcttttt cggtgtagaa caggcaaact gggacaactt cacgcttgaa ggcggcgtac
     4501 gcgtggaaaa acaaaaagcc tccattcagt acgacaaagc attgattgat cgggaaaact
     4561 actacaacca ccccctgccc gacctcggcg cgcaccgcca aaccgcccgc tcattcgcac
     4621 tttcgggcaa ctggtatttc acgccacaac acaaactcag cctgaccgcc tcccatcagg
     4681 aacgcctgcc gtcaacgcaa gagctgtacg cacacggcaa acacgtcgcc accaacacct
     4741 ttgaagtcgg caacaaacac ctcaacaaag agcgttccaa caatatcgaa ctcgcgctgg
     4801 gctacgaagg cgaccgctgg caatacaatc tggcactcta ccgcaaccgc ttcggtaact
     4861 acatttacgc ccaaacctta aacgacggac gcggccccaa atccatcgaa gacgacagcg
     4921 aaatgaagct cgtgcgctac aaccaatccg gcgccgactt ctacggcgcg gaaggcgaaa
     4981 tctacttcaa accgacaccg cgctaccgca tcggcgtttc cggcgactat gtacgaggcc
     5041 gtctgaaaaa cctgccttcc ctacccggca gagaagatgc ctacggcaac cgtcctttca
     5101 tcgcacagga cgaccaaaat gccccccgtg ttccggctgc gcgcctcggc ttccacctga
     5161 aagcctcgct gaccgaccgt atcgatgcca atttggacta ctaccgcgtg ttcgcccaaa
     5221 acaaactcgc ccgctacgaa acgcgcacgc ccggacacca tatgctcaac ctcggcgcaa
     5281 actaccgccg caatacgcgc tatggcgagt ggaattggta cgtcaaagcc gacaacctgc
     5341 tcaaccaatc cgtttacgcc cacagcagct ttctctctga tacgccgcaa atgggccgca
     5401 gctttaccgg cggcgtgaac gtgaagtttt aaaatcggac aggcaaacaa aaaaggtttt
     5461 cagacggcat tctgaaaacc ttttttcttt ttaatcaatt tgttaggaaa tatggcaaag
     5521 acaaagaaca aaccactatg ccatcattcc catttttttt gtttcatcat tcttactttc
     5581 acgccgtcat tctcgcaaaa gcagaaaaat caaaagcaga aacccaagat cccgtcattc
     5641 ccgcgcaggc gggaatccaa ttcgttcggt ttcagccgtt tccgataaat cgccttagca
     5701 tttgggttct agattcccac tttcgtggga atgacgcgga aaggtttctg tttttttaag
     5761 ttttaggtaa ctaatgtgcc atcattcccg cgcaggcggg aatccaattc gttcggtttc
     5821 agccgtttcc gataaatcac tgtaacgtta agttcccgga ttcccacttt cgtgggaatg
     5881 acggcgtaaa ggtttctgtt ttttaagttt taggtaacta ataagtcgtc attcccgcgc
     5941 aggcgggaat ccagtctctt cgttttcagc cgtttccgat aaatcgcctt agcatttggg
     6001 ttctagattc ccactttcgt gggaatgacg cggaaaggtt tctgtttttt aagtgttagg
     6061 taactaataa gtcgtcattc ccgcacaggc gggaatccaa ttcattcggt ttcagccgtt
     6121 tccgataaat cgtcgtaacg ttaagttccc ggattcccac tttcgtggga atgacgcgga
     6181 aaggtttctg ttttttaagt gttaggtaac taataagtcg tcattcccgc gcaggcggga
     6241 atccagtccg tccggtttca gtcgtttccg ataaatcgtc gtaacgttaa gtttctagat
     6301 tcccactttc gtgggaatga cggtgtgaac attagcaaaa attcaaaaaa ccaaacttcc
     6361 tcattcgtgt gaatgatggt actgatattt tctatttcag tccgcaaaat taatctttca
     6421 acacatcttg cacagccgcc tgaatcttag ccgccgctgc ctcgatttgc cgttgttcgg
     6481 cagatgggtt tgccgctgtt tcaaccggag cagtttgtaa agcaggctca gattcagttt
     6541 gcggcgcagt cagcggcaaa cgcgccaata cagtgctgac gccggttact ttatcgccaa
     6601 tcgccacttg cgcctgcgca tcgacaggca gatacatatc cacgcgcgaa ccgaagcgga
     6661 taaagccata acgttcgccg cgggacagtt tcgcacctgc ttgggtgtag cacaaaatac
     6721 ggcgcgccac caaaccggcc acttgaacaa aagtaatttc acgacctgaa gccgtagtcg
     6781 ccaacaccgc attacgttcg ttttccgtgc tggctttgtc caaatccgca ttcacgaatt
     6841 tgcctttgtt atagaccact ttcgttaccg tacaatcggc aggcgatttt tgcgaatgca
     6901 cgttgaacac gttcataaaa atactgattt tcaaagcatc gacatcacga tacggatcgc
     6961 gtgcgcgttc gaccaccacg atacggccgt caaccgggct caacaccgct tcaggattta
     7021 gcggaatctc acgcgcaggg tcgcggaaaa actgcaatgc aaatacggta aacacccaaa
     7081 acggcaaaga ccaccagccg caacatatcg acaccagcag gctcaaagcc aaaccgccgc
     7141 caataatcgg ccagccctca cgggcgataa tcgggtgggg gtaaagacgg ttcatttgtc
     7201 atccttttgt tcaagcgggt taaaattgcc gacattatag cggaatctgc cgtactccgc
     7261 tggcaaatgc cgtctgaacg cggcttcaga cggcatccga gaaagtgcta caataccggt
     7321 tttcctttat ttttcaggcg gcatccatta tgtgcaacca tcatccccaa cattcgcacg
     7381 acaacgacac catccgcatc cgcggcgcgc gcacgcataa tttgaaaaac atcgatttag
     7441 acattccgcg ccacaaactc gtggtggtaa caggattgtc gggcagcggc aagtcgtcgc
     7501 tggcgtttga cacgctgtat gccgaagggc aacggcgtta tgtcgagagc ctttccgcct
     7561 atgcgcggca gtttttgcag atgatggaca aacccgatgt cgatttgatt gaaggtctgt
     7621 cgcctgcgat ttccatcgag cagaaatcca ccagccacaa cccgcgttcc accgtcggca
     7681 cggtaacgga aatccacgac tacctgcgcc ttttgtacgc ccgcgtcggc acgccgtatt
     7741 gccccgaaca caatctgccg ttgtcgagcc aaaccgtatc gcagatggtc gatgccgtct
     7801 taaaactgcc ggaaggcacg cgcgtgatga ttctggcgcc ggcggtgcgc gagcgtaagg
     7861 gcgagtttgt cgatttcttt gccgacttgc aggcacaggg ttttgcgcgc gtgcgcgtgg
     7921 acggcgaagt ctatcagttg gacgaagtgc cgaagctgga aaaaaacatc aaacacaaca
     7981 tcgacgtggt catcgaccgc gtgaaagtga aggcggacat caagcagcgg ctggcggaaa
     8041 gttttgaaac cgcgctgcgc cacggcaacg agcgcgcgct ggcgatggag atggacagcg
     8101 gcgaagaaca ttggttttcc gcgcgttttg cctgccccgt gtgttcgtac agcctgcccg
     8161 aattggagcc gcgcctcttt tcgttcaata atccgatggg ctcctgcccg acttgcgacg
     8221 gcttgggcaa caccaatttc ttcgaccccg aaaaagtggt cgcccacccc gaattgtctc
     8281 tggcttcggg cgcgattgac ggctgggaca agcgcaacca gttctatttc caaatgattc
     8341 agtcgctggc acggcattac agttttgatg tgcaggctgc ttgggaaacc ctacctgcaa
     8401 aaatcaaaaa agtcgtgttg cacggctcgg gcaaggaagt cattgatttc acctacctat
     8461 ccgaacgcgg caccaccttc aaccgcagcc acgccttcga aggcatcatt cccaatctcg
     8521 aacgccgcta ccgcgaaacc gacagcgaaa ccgtgcgcga aaaactgcgc gaataccaaa
     8581 accaccgcgc ttgcccgagc tgcggcggcg cacgtttgcg caaagaagcc cgctacgttt
     8641 acgtcggcgg cgagccgttg cacgaagttt ccgcctggcc gctgaccaaa acccaccgat
     8701 tctttgaaac gctggatttg gacggcaaca aaaaacaaat cgccgaaaaa atcctcaaag
     8761 aaatcaccga acgtctcggc tttttgatta atgtcgggct ggattacctg aatctctccc
     8821 gttccgccga aaccctttcc ggcggcgaag cccagcgcat ccgcctcgcc agccaaatcg
     8881 gcagcggcct gaccggcgtg atgtacgttt tggacgaacc ctccatcggc ctgcaccagc
     8941 gcgacaacga ccgcctgctt gccaccctca aacgcctgcg cgatttgggc aacagcgtca
     9001 tcgtggtcga acacgacgaa gacgccatcc gcgaagccga tttcgtcgtc gatatgggcc
     9061 cgggcgcagg cgaacacggc ggcaacgtat tgattgccga cacgcccgaa aacgtcgcca
     9121 aatgcgaaaa ctccgttacc ggacaatacc tcagcggcaa aaaatccatt gccgtgccgt
     9181 ctgaacgcac gcccgtcaat cccgaccgaa tgctcgtcct caaaggcgcg cgcggcaaca
     9241 acctcaaaaa cgttaccctt gagctgccgc tcggtttgat tacctgcatc accggcgtat
     9301 ccggcagcgg caaatccacc ctgattaacg acaccctcgc caaaatcact gcccgcgaac
     9361 tcaaccgcgc ccaagaagaa cccgccccat acgacgacat ccgcggcctc gaacacctcg
     9421 acaaagtcat caacgtcgac caatccccca tcggtcgcac cccgcgctcc aaccccgcca
     9481 cctacaccgg cctgttcacc cccatccgcg aactcttcgc cggcgtaccc ctctcgcgcg
     9541 aacgcggcta caacgtcggc agattctcct tcaacgtcaa aggcggccgc tgcgaagcct
     9601 gccaaggcga cggcgtgatt aaagtcgaaa tgcacttcct gcccgacgtg tacgtcccct
     9661 gcgaagtctg ccacggcaaa cgctacaacc gcgaaaccct cgaaatccaa tacaaaggca
     9721 aaaacatcag tcaagtcctc gacatgaccg tcgaagaagc ccgcgaattc ttcgacgccg
     9781 tccccaccgt atcgcgcaaa ctgcaaacct tgatggacgt aggcttaggc tacatccgtc
     9841 tcggccaatc cgccaccacc ctctccggcg gcgaagccca gcgcgtcaaa ctcgccttgg
     9901 aactctccaa acgcgacacc ggcagaacgc tctacatcct cgacgaaccc accaccggcc
     9961 tacacttcgc cgacatcgcc ctgctgctgg aagtcataga ccgtctgaaa ggcaaaggca
    10021 actcgatagt gattatcgag cataatctgg atgtgattaa aaccgccgat tggattgtgg
    10081 acttagggcc agaaggcggc gacggcggag ggaggattat tgcttcaggt agcccggagg
    10141 aagtggcgaa ggttaagggg agttatactg ggaagtattt gaagaatata ctttaaaaaa
    10201 cgggatggtt taaaccatcc cgttttttta ttccttctgt tttcgatttt gtttggcttt
    10261 gaagctatca aaaataatgc taaatcccaa acaggtgaac attaaaggca tcccccattt
    10321 catatccgaa gaaataacat taaacatttc taataatgta agaatgcctg ataaagctat
    10381 aaaaatacct aaaaacatta gaactatcct tattatctgg agctatctgg agttctttta
    10441 atttgaatcc atgagcttcc tggattttga gtgggtggtg ctttttgatt atctttaatg
    10501 gttgcgtagt tatcttttcg accacctcgt gaaccaattt cttgataaat tccaccgtta
    10561 tttcctgtat tttgaccagg tttgattttg ttcgtcatta tcatctccat aatttaaata
    10621 gttaaagtat gagtccattg cggataccaa tttgcagata aaaaatctac tagatttttg
    10681 tctccataga attgtgtacg cccatgttgt tcaaatatta acgcagattg gtcaattttt
    10741 aatccatttc tacgagcttc agtggtcaat tccttcagta cgacactatg gttaccatta
    10801 gttgatttag catcaaaaac agcaaagtta atatatcctc cacctacggc tggatgtctt
    10861 aagtgagcaa attttatttt cataaataaa tcctataaaa aattacataa cagaaatttt
    10921 aagtcagaga gctactatga agtagctttt tgcattatat gcactctttt tgattacgtc
    10981 aagcaatttt tttgaattca tattgccatg aaaaagattg atggaatata atgcaattat
    11041 gaaaacaatc gctccttccc atataaagcc ccttatgcca gagttgaacc aatctcaacg
    11101 caataggatt atatttattg atttctatct atgctttcta ggaaagataa atagaaaaga
    11161 tttgatgcag gcatttcaaa tttccccagc tgttgctact agggattttg cgctatattg
    11221 ctctagtttt tcgaaaaatc tccgttttga taccaaaagt aaattttacc tctctactga
    11281 agactttgaa cctcaattcc cccattcatt tgaaaatgca attaaaattc ttaatggcag
    11341 tttaatattt gatattgatt cccaacatca acctcttctt ccgtttgatt tacctcctgt
    11401 tttaataaat cctgatgtct taatcttttc taaaatatcg aaagcattat ccttaaaaaa
    11461 acctctaaaa atctcatatc attcaatttc atctggatta tctgagaaag ttattgtgcc
    11521 tcttgctcta gttaatgacg gatttcgatg gcatttacgg gcattcgata gaacaaaaaa
    11581 ccgatttgca gattttgtcc taacacgaat aattcaagca aatattttat tagctgaaaa
    11641 tattgaagaa aatgaaacga ttaacaatga tattgattgg aatagaattg ttaaaatcga
    11701 gttggttccc catcctaatc atgatgaagc taaaaaagtg attgaaatgg actttggaat
    11761 gacaaatgga aaattagaat tggcgcttag agcttcaaat atcggatatg ttttacagca
    11821 atggcgagtc gattgttcgt cagatcatca tatcgatgat cctgctttta gattatggct
    11881 gttagataat ttagttttat acggcatcaa caatgctgaa cacttagcgc ctgcctttga
    11941 taaaaaacgt agcaagccgt agcctacata tccaaccgac cgcgtgcgtg gctgcgccac
    12001 acacgcggtt gtttgattga tggctttctg ttgtaaattg aacttagtta aaactaacac
    12061 tttcagacgg catgaaaaat ggccgtctga aaactgaaaa agctttatct aaaacaacta
    12121 ccctaactca caaaacaaaa aacggattcc caatcattgg aatccgtttt tctgtaaagc
    12181 tatccgaatt atttagcttc ttctttagcg tcttcagcag cttctttggc atcttccgct
    12241 gcgtcttttg cttcttcttt agcctcttca gcggcgtctt tggctttttc ttcaggagtt
    12301 tctgcagcag caagccgtag cctgcatacc caacaccgcg tgcgtggctg cgccacacac
    12361 cctacctgtg aattacacgg aacttcaaaa tcaacgtgta gggtgtgtgc ggtacgcacg
    12421 cacgcggttg cttgattgac ggtttgccgt ttcaa
//
LOCUS       DAXIEB010000058        12449 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000058 DAXIEB010000000
VERSION     DAXIEB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.058"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(26..124)
                     /locus_tag="ACFOA3_001277"
                     /pseudo
     CDS             complement(26..124)
                     /locus_tag="ACFOA3_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(229..726)
                     /locus_tag="ACFOA3_001278"
     CDS             complement(229..726)
                     /locus_tag="ACFOA3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFC6898282.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            complement(812..1234)
                     /locus_tag="ACFOA3_001279"
     CDS             complement(812..1234)
                     /locus_tag="ACFOA3_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898283.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            1546..2856
                     /locus_tag="ACFOA3_001280"
     CDS             1546..2856
                     /locus_tag="ACFOA3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6898284.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLAQILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            2970..5975
                     /locus_tag="ACFOA3_001281"
     CDS             2970..5975
                     /locus_tag="ACFOA3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC6898285.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREAISSQYK
                     AQLQAALAQLEVEKGSFDRKEIYAHPHFVRPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQTASNHNLTAALILHDVGTKDERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTAKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIAILETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6024..7013
                     /gene="rhuM"
                     /locus_tag="ACFOA3_001282"
     CDS             6024..7013
                     /gene="rhuM"
                     /locus_tag="ACFOA3_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFC6898286.1"
                     /translation="MYNNDIIIYTTEDGLSEFTLRELDGELWLTQKEIAELYQTSKQN
                     IGKHIKAIFAEQELDDSVVNFQFTTAADGKNYRMGLYPLSLIIAVGYRVRSTRGTQFR
                     QWATRTLGEYISKGFVLNDDRLKNPPVGTNRAEDQFEQLLNRIRDIRSSERRMYLRVR
                     EIFAMAADYQPSFKETTHFFKIIQNKLHYACTRYTAPEIIYQRADAGKPNMGLTHWRG
                     KEIAKADVTVAKNYLNEAEIDALNRIVSMWLDFAEDQASMKKQIFLQDWAEKLDAFLA
                     FNDRQVLQTAGTISKTQADEKARIEYEKFAAARRLEKEREGERHIAELLNLKK"
     gene            7107..8264
                     /locus_tag="ACFOA3_001283"
     CDS             7107..8264
                     /locus_tag="ACFOA3_001283"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6898287.1"
                     /translation="MNSALKNHATYINNKVDTNEISLSQYISTDNILQNKQGIECATS
                     LPIQGGKVTSFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANTETDSHFLFYA
                     LFRDDFFIHAMKGAKGTKMPRGDKTQIMEFKIPVFDLKTQQSIAAVLSTLDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKREIPKGWGSIELQ
                     SCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDAHIIFGDHTR
                     IVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFKII
                     LPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQVSVAE"
     gene            8312..9913
                     /locus_tag="ACFOA3_001284"
     CDS             8312..9913
                     /locus_tag="ACFOA3_001284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6898288.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREHLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDKVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            9945..10061
                     /locus_tag="ACFOA3_001285"
     CDS             9945..10061
                     /locus_tag="ACFOA3_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6898289.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(10221..11558)
                     /gene="ribBA"
                     /locus_tag="ACFOA3_001286"
     CDS             complement(10221..11558)
                     /gene="ribBA"
                     /locus_tag="ACFOA3_001286"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6898290.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            11667..11795
                     /locus_tag="ACFOA3_001287"
     CDS             11667..11795
                     /locus_tag="ACFOA3_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898291.1"
                     /translation="MPSEAGFVASDGIAAIPAGGAAVSSRPLPIRDLPEAYSEKNF"
     gene            complement(11774..>12449)
                     /locus_tag="ACFOA3_001288"
     CDS             complement(11774..>12449)
                     /locus_tag="ACFOA3_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6898292.1"
                     /translation="ADKKVDLILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYI
                     ANAWTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGNS
                     ISHNIKYKNFSDMLAHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSK
                     NIIVIYRKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVY
                     QKFFSE"
BASE COUNT         3483 a         2990 c         2896 g         3080 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactataaa ctgacgcaaa tgccgttttg
       61 tacaattcca aaagttttca attccgttaa tgcgattttg ccgtttggcg aaatgcgtac
      121 tgttccagtc gtggattgaa cccccaccct gtatagttct ttcgaagcat tggggtattg
      181 ttttttcaaa gcatcgatta aagccgtatt ggtgtccgga tttttaacct agacaatatt
      241 gtccccttcg gcttcaagcg ttgcttttgc agaatgcgac gcgataatgc cttttacgga
      301 ggcgtggttt tttacattta aatcaacttc attatttccc ttgacggctt tgagcttgat
      361 ggtgctgatg ccatcaaggg aaggttgggt aaggtcgctt gattgattat tgatgaaaat
      421 gcccgtcgtg tgtccggaat agggggagtt ggactggaga ccatttacgc ccaatcccaa
      481 gcccttgccc tcggtggcga tggatatttt attgttattc cccgttaata cggtattgga
      541 tttaaaagcc gtttcaatgc cccttaaaaa acttgtactc tcaacatttc ctgtcggcat
      601 aaggtcggcg agttcccctt taatctcaat cacattatcg ctgtcttcgg cagtcagatg
      661 aacatcggta ttgttgaggt tgtgaatacc gaaatggtgt gtgtctttat gaactttaac
      721 gttcatattg aacaaattgc ctgtttttga atgaaaggat atgctgcctt tgtcattttc
      781 atcggggggc tgatagtgtt gacataccgc actaaggcac tattgtttgt cggtattttt
      841 ttccactggc tacatcttgt accccatcaa catcgggaac attcaaatta aattgaattt
      901 ttcctttggc atccaaatgg atatttccgg aatggggctt cacgtcaaca gattggaaat
      961 tatttttaaa tagaatatca ttattgcttt ttaccaataa ttcaggcgta tatcccaatt
     1021 gagagtctgc aaaagattgg atgccgtaaa ttccatttac cgttaaattt ccattatcat
     1081 ttttaccagt ggtaataagt tctaattttc taaagttttc accttttttc caccaaggca
     1141 caatatagtt ttttacagtg attttttcat ctttaagggt tataagacta tcatcgtctc
     1201 tttgcggatc agaaaataaa gacacccatt ccatctcttg aatattagta tattcatggg
     1261 cttccgcctc aaaacataat gccgattgaa tcaacaaagc aatggtagat aaagcaaatt
     1321 ttctatttta gtctccttaa tgtttaattg aaacgctgat ttgggtattg tcatgccggt
     1381 gcggaatcgg tctccgtgcc ggatatattg tgtgaagtct ctcgtctttt ccagtatgcg
     1441 cctaatatcg gcttaaggca aacggcgggg cggaatccgt atgcggcacg ccgccgttcc
     1501 ctgccgtata atcatattgt ttgaaaacct gttgacggag ccgttatgac cgatttgttt
     1561 gcccgagaac ccgacgcgcc gcttgccgaa cgattgcgtc cgcatacgct tgacgacgtg
     1621 gtggggcagg aacacctcat cggcgaaggt aaacctttgc gcgtggcggt agaaggcggc
     1681 aagccgcatt ctatgttgct gtgggggccg ccgggcgtgg gcaagacgac gttggcgcag
     1741 attttggcgc agagtttcaa cgcccagttt ttgcctgttt ccgccgtatt ttccggcgtg
     1801 aaggacatcc gcgaggcaat cgataaagcc gaaatcgctt tgcagcaggg acgcgcgacg
     1861 attttgtttg tcgatgaagt ccaccgcttc aacaaggcgc agcaggacgc gtttttgccg
     1921 catgtcgaaa gcggtttgct aacctttatt ggtgcgacga cggaaaatcc gtcgtttgaa
     1981 gtcaatcccg cgctgttgag ccgcgctcag gtgtatgttt tgcaaccctt gtcttcagac
     2041 gacctgaaaa agctgattgc caaggtattg gctttgcctg aataccaaga gtttacgatt
     2101 gaaacggatg cgcaaaaatt actcgtgaat accgccgacg gtgatgcgcg cagattgttg
     2161 aatttgttgg aacaactttt acgcgccgcc gatacacgtc gtctgaaaaa cttaaccgcc
     2221 gaatttctcg ccgacagtct cggggcgcaa atccgccgtt tcgacaaagg cggcgagagt
     2281 ttctacaacc aaatctccgc cctgcacaaa tccgtgcgcg gttcgcatcc gaacgccgcg
     2341 ctgtattggt tctgccgtat gctcgacggc ggcaccgacc cgcgctacct cgcccgccgc
     2401 atcgtgcgta tcgcttggga agacatcggg cttgccgacc cgcgcgcctt ccaaatcgcc
     2461 aacgatgccg ccgccacctt cgaacgctta ggctcgcccg aaggcgaact ggctttggcg
     2521 caagcggtat tgtatcttgc cgccgccgcg aaatccaacg cgggctacaa ggcatacaac
     2581 caaatgcgcc gtttcgtcaa agaaaacgcc agcgacgaag tacccgtcca cctgcgcaat
     2641 gccccgacca agctgatgaa ggaattgggc tacggacgcg aataccgcta cgcccacgac
     2701 gaaccgaacg cctacgccgc cggcgaaagc tatatgcccg acggcttgga cgaaccggac
     2761 ttctaccaac ccgtcccgcg cgggttggaa atcaaaatcg gcgaaaagct ggcgtggctc
     2821 aaatcgctgg atgaagaggc attaaaggca aaatgaagca atgccgtctg aagcagaaca
     2881 gtatggcaaa gccggtacac cgaaaaggcg tatcgggttt ttcaggtgtt ataatccgtt
     2941 tcaataaatt ttgatgaatc attacgttta tgtcccaaac tcacaacgaa aattcccgcg
     3001 tcaaaatccc cgccgttttg cacctgatgc gcttgggcta cgactacctc tcgctcaaaa
     3061 acgaaaattg ggataaacca accaacatct ttcccaaaat ctttatagaa agcctctgcc
     3121 gcatcaatcc cgatttgtcg ccggacgatg cacgccgttt gctgaccgac atacgcttag
     3181 aactggacaa cgaagacttg gggcaaaagt tttacgaacg ccttaccaat caatccggcg
     3241 gcaagaaact gatcgacttc caaaattttg ataacaacag cttccacgtt gtaaccgaat
     3301 tgccctgtat caacggcgat gaagaattcc gccccgacat taccctgctg gtaaacggca
     3361 tgcctttggt ttttatcgaa gtcaaaaagc ccaacaacaa aggcggaatc ggcgaagagc
     3421 gggagcgcat gggcaaacgt gcgaaaaatt ccaaatttcg ccgtttcatc aacatcaccc
     3481 aattcatgat tttttccaac aacatggaat acgacgacgg cgcaaccgaa ccggcacaag
     3541 gcgcgtttta cgcttcgtct gcttacggta agcccgtgtt taactacttc cgcgaagaac
     3601 ataaattaaa tcttgccgaa ttattgaaca cgctttcaga cggcctcgaa aacaacgtcc
     3661 tgcaagacaa caacctgccc gttatcaaac acagccccga atttatcagc aataaatcgc
     3721 ccgatacgcc gaccaaccgc atcctgactt cgctgctttg ccgcgaacgt ctttcttttc
     3781 ttctgcaaca cgggctgact tacgtcaaag caagtcaagg cttggtgcaa aaacacatca
     3841 tgcggtatcc gcagctattt gccactctcg ccattgaaaa acatttggca aacggcggca
     3901 aaaaaggcgt gatttggcac acccaaggct ctggcaaaac cgcgcttgcc tattacaaca
     3961 cccgctacct gacccactat tatgccaaac agggcatcgt gccgaaattc tatttcatcg
     4021 tggacaggct tgatttattg aaacaggcgc agcgcgaatt tactgcccgc gatttagtcg
     4081 tccataccat cgacagccgc gaagcctttg ctgccgacat caaatccgcc caaactttgc
     4141 acaaccacgc aggcaaagcg gaaatcactg ttgttaatat ccaaaaattc caagacgacc
     4201 ccgatgtcgt cgctcgtaac gactacgacc tcgccattca gcgcgtctat tttctcgacg
     4261 aagtacaccg cagctacaac cccaaaggct cgtttcttgc caacctcaac cagtccgacg
     4321 taaacgccgt caaaatcggg ctgactggca cgccgcttat cggcgtaacc gcaggcaacg
     4381 tcaacacccg cgaactcttc ggcgactaca tccataaata ctattacaac gcctccatcg
     4441 ccgacggcta caccctgcgc ctgattcgcg aagccatcag cagccaatac aaagcccaat
     4501 tgcaggctgc actggcgcag cttgaagtcg aaaaaggcag ctttgaccgc aaagaaatct
     4561 acgcccatcc gcatttcgtc cgcccgatgc tcgattacat cttggacgac ttcgccaaat
     4621 tcagaaaaac caaccaagac gacagcctcg gcgccatggt cgtttgcgac agcgcagaac
     4681 aggcacgcca gctttttgag cattttcaga cggcctcaaa ccataattta accgccgcgc
     4741 tgatattgca cgacgtcggc actaaggacg aacgcgatca atgggttaaa gacttcaaag
     4801 caggcaaaac cgacattttg tttgtgtaca acatgctttt gaccggcttt gacgcgccgc
     4861 gcctgaaaaa actctatttg ggcaggctca tcaaagccca caatcttttg caaaccctga
     4921 cccgtgtcaa ccgcacctac aaatcttacc gttacggcta cgttgtcgat tttgccgaca
     4981 tcgaacgcga attcgacaaa accaaccgcg cctattggga tgaactttct aatgaattgg
     5041 gtgacgaaat cggcagttac agccagcttt tcaaaactgc cgaagaaatc gaacaggaaa
     5101 ttgcagacat taaaaacgcc ctgtttgatt tcgataccga aaacgccgaa gaattttgca
     5161 gccaaattag ccaaatagca gacaaaaaac agctgctcgc cctaaaaaaa gccctgcaaa
     5221 ccgccaaaaa gctgtacaac gtcctgcgcc tgcaaggcag ccacgaattt ctcgcccatc
     5281 tcgattttga caagctcaat cttttgtacc gcgaaaccgc cgcgcgtttg gagaccttaa
     5341 accttgccga aaaactacag caaggcgaca ccgcccattt gctcaatgaa gcgctggaag
     5401 acgtttactt tcaattcgtt aaaatcggcg aagccgaact caaacttgcc gacgatttga
     5461 aagacatcat gcgcaaagtc cgcgaagggc tggcgggcaa cttcgatcaa gaagaccccg
     5521 aatacatcag cctgcgcgaa gaactggaac gcatcttcaa gaaaaagaac ctcgccgaag
     5581 tcgggcaaga agaaatgcag gcaaacatcg ccatactcga aaccgtctat gccaaaatca
     5641 aagagctgaa ccgccaaaac gacctcttgc ggcacaaata cggcggcgac gccaaatatg
     5701 cccgcatcca caaacgcctg cgcgaaaacc ccgccctcta cggcgataaa ctcaaagtct
     5761 ttaacgcgct gaatggtgta aaaaccgaca cggaccaaaa agtgctggat atggagcaga
     5821 ttttggacaa ccaaaattac tttgaaaaac aaatgcaggg catcgtatta aaacgcttta
     5881 gaacggaaca gcaatttccc gttcaacccg cagacatcca agccatcaac cgcctgttgg
     5941 tgcgggaata tttaaaagaa agtgggcgga tttgaggagt agattttcag gctgccttaa
     6001 ccatttataa atacaggaaa gctatgtaca acaacgacat catcatctac accacggaag
     6061 acggactatc cgaatttacc ctgcgcgagc ttgacggcga actatggctt acccaaaaag
     6121 aaattgccga actctaccaa accagcaaac aaaacatcgg taaacacatc aaagccatat
     6181 ttgcagaaca agaactggac gattcagttg taaactttca gtttacaact gctgcagacg
     6241 gcaagaacta ccgcatgggg ctttatcccc tctccctaat catcgccgtc ggctaccgcg
     6301 tccgctccac acgcggtaca caattccgcc aatgggcaac acggacattg ggcgaataca
     6361 tcagcaaggg cttcgtatta aacgacgacc gcctgaaaaa cccgcctgtc gggacaaacc
     6421 gtgccgaaga tcagtttgaa cagttgctca accgcatcag ggacatccgc agcagcgaac
     6481 ggcggatgta tctgcgtgtg cgcgaaatat tcgccatggc ggcggattac cagcccagct
     6541 tcaaagaaac cacccatttt ttcaaaatca tacaaaacaa acttcattac gcctgtaccc
     6601 gttataccgc ccctgaaatt atttaccaaa gggcagatgc ggggaaaccc aatatggggc
     6661 tgacccattg gcggggcaaa gaaatcgcca aagccgatgt aaccgttgcc aaaaattacc
     6721 tgaacgaggc agaaatcgac gccctcaacc gcatcgtttc catgtggctg gattttgccg
     6781 aagaccaggc aagcatgaaa aaacagatat tcctgcaaga ctgggcggaa aaactggatg
     6841 ccttcctcgc cttcaatgac cgccaagtct tacaaacggc agggacaatc agcaaaacgc
     6901 aggcagacga aaaagcccgc atcgaatacg aaaaatttgc cgccgcacgc agactggaaa
     6961 aagaacgcga gggcgaacgc catattgccg aactgctgaa cctgaaaaaa tagcaggcat
     7021 caatgcaggc tgcttaaaaa agtgcggtcg gattttgaaa tgttttttca gacggccttt
     7081 gaagagtttt tagagataca gttaagatga atagtgcatt aaagaatcat gcaacatata
     7141 taaataacaa agttgataca aatgaaattt cgcttagcca atatatttca acagacaata
     7201 tattgcaaaa taagcaaggt atagaatgtg caacgtcttt accaattcaa ggtggaaaag
     7261 taacttcatt taaaaagggc gatattttat tagccaatat acgaccatat ctaaaaaaaa
     7321 tttggtatgc ccaatttgat ggaggttgtt ctgctgatgt tttagcgatt cgtgctaata
     7381 cagaaacaga tagtcatttc ttattttatg ctttgtttcg tgatgatttt tttatccatg
     7441 caatgaaagg tgcaaagggt acaaagatgc cacggggcga taaaacacaa attatggaat
     7501 ttaaaattcc agtttttgac ctcaaaaccc aacaatccat agccgctgtc ctatctactt
     7561 tggacaaaaa aattgccctg aacaaacaaa tcaacgcgcg cttggaagag atggcgaaaa
     7621 ccctgtacga ctactggttc gtgcagttcg acttccctga cgccaacggc aagccctaca
     7681 aatcctcagg cggcgaaatt gtgtttgatg aaaccttgaa acgggaaatt ccgaaggggt
     7741 ggggaagtat agaattacaa tcttgcttag caaaaatccc aaatactaca aaaatattaa
     7801 ataaagatat taaggatttt ggaaaatatc ctgttgttga ccaaagtcaa gattttattt
     7861 gtggatttac caatgatgaa aaatctatat taaacccgca agatgctcat attatttttg
     7921 gtgatcatac aagaattgtt aagttagtta attttcaata tgcgcgggga gcagatggta
     7981 cacaagtaat attaagcaat aatgaacgaa tgccaaacta tttgttttac caaatcataa
     8041 atcaaataga tttatcaagt tatggatatg ctagacattt caaattctta aaagaattta
     8101 aaataatttt acctagtaaa gatatttctc aaaaatataa tgaaatagca aatactttct
     8161 ttgttaaagt tcgaaataat ataaaacaaa accaccaact aacccaactc cgcgatttcc
     8221 tacttcccat gctgatgaac gggcaggttt ctgtggcgga ataagccaaa ttcaagaatc
     8281 agacctacaa aataaaacaa acgagaaaaa aatgaccgaa caacacttta ccgaacaaac
     8341 caaatcccta atcgacagct taaaaaccat ttgcgccaac tatggtttgg gcaatgacgg
     8401 caacgagttc aaaatcatca gtcaggcatt tttgtataaa ttcctaaacg acaaatacga
     8461 tttcgaagtt aaacaaattc gcaaagaaaa tcccgacgag ccaatcgaat tcatcaatat
     8521 ggatattgag ggaaaaacag ccgttttgaa gcctgaacat tccatcaagt atttatcaga
     8581 gcgacaaaac ggcgcggact ttgccaaact ctttgacgat acgctgaccg atattgccgc
     8641 acataatgcg gaactgtttt cggtaaaaac cgaaggcggg gcgaaaatcg tgttgttcga
     8701 gcgtatcagt caatatatta cggatgaagg ccgccgcgac gatttttgcc gtgcgctgat
     8761 ttccaaactg gcgggtttta gtttcgaggc gatttttgcc caaaaatttg atttttttgc
     8821 cacgattttt gaatacctga ttaaagacta caacagtaat tcgggcggca aatacgccga
     8881 atactacacg ccgcacgcag tagcgcggat tatggcggat attttggtgc cggaggaagt
     8941 gcgcgggcaa atccgcagcg tggacgttta cgatccgtcg gccggttcgg gaacgctgtt
     9001 gatgaatgtc gcccatgcca tcggcgaaga taaatgcatg atttatacgc aggatatttc
     9061 gcaaaaatcg tccaatctgc tacggctgaa tctgattttg aataatctgg tgcattcttt
     9121 gaataatgtg gtgcagggca atacgatttt gtcgcccgcc cataaagacg cttcaggccg
     9181 tctgaaaaaa tttgatttca tcgtttcgaa tccgccgttt aaattggatt tcagcgattt
     9241 ccgcgaccaa ctagaaggcg aggaaaaccg cgaacgcttt tttgccggta tcccgaaaat
     9301 caaggcgaaa gatacggata agatggaaat ctaccagctt tttatccagc atatcttgtt
     9361 ttccctgaaa gaaaacggca aagcggcgat tgtgttgccg acgggcttta tcactgccca
     9421 atcaggcatc gacaaaaaaa tccgcgaaca tctggtagaa aacaaaatgc tcgccggtgt
     9481 ggtttccatg ccttccaata ttttcgccac cacgggaacc aacgtttcca tcctgtttat
     9541 cgataaagcc aataaagata aagtggtatt aatcgacgca tcgggcttgg gcgaaaaaat
     9601 caaagacggc aaaaaccaaa aaaccgtact ttctcgcgaa gaagaacaaa aaatctgcaa
     9661 taccttcacg cacaaacagg cagtggaaga tttcagtgtg gtaatcggct acgatgaaat
     9721 caaagcgaag aatcacagct tgtcggcggg gcagtatttc gaggtaaaga ttgattatgt
     9781 ggatatttcc gccgacgaat ttgcgcaaaa aatggcggga ttttcagcgg atttggataa
     9841 acttttcgcc gaatctgccg aattggaaaa agagattaag gatagattgg cgatgttgaa
     9901 attcaattca taaataccga tgaatgaaac cggtcaaact taaaatgccg tctgaaagcc
     9961 tttcagacgg cattttgttt ggtttggaaa atacttatgc cgttctttcc ctgaaagaga
    10021 gaatccaaaa aaccaaagcc acaggaattt atcggaaatg acaaaaaccc gacgaaccgg
    10081 attcccgcct gcgcgggaat gacgaattag aagttacccg aaatttgcaa aaacaaaatc
    10141 caacccaaca gaccgggttt tcgtttgcac ggaaataatg caataaataa agcaaatata
    10201 aagtatttga atttactata ttatttttcc gcttcttcaa agccgacgac ttccaaaccg
    10261 aagccggtca ggccggtgaa agatgagggc tgcccgagga cgcgcagttt tttgacgttg
    10321 aggccggcga ggatttgtgc gccgatgccg tagcttttgc tgtcccattt gtaggcttgg
    10381 tttgcgcctt tgggcagggt gcggtcgagc agggatgcgc cgtcttcggt gcggtgcaga
    10441 aggatgacga cgccgctttc tgcctgttgg acgcgctcga gtgctttggg cagcgaccat
    10501 gaatgacgcg ggttagcttg gatgaagtcc atgacgctga agggttcgtg gacgcggacg
    10561 agggtttcgg tgtcggcgga gggcgtgcct ttgacgaggg cgaggtgggt ttcgccggag
    10621 agtttgtcga cgtaaacgtg ttgttggaac tcgccccacg gggtttgtac aggcgcattg
    10681 cccatgtctt caagcaggct ttcggtacgg ctgcggtatt cgatgaggtc ggtaatggtg
    10741 ccgattttga gcttgtgttc ttcggcaaac ttcatcagtt cgggcatacg cgccatcgtg
    10801 ccgtcgtcgt tgatgatttc gcaaataacg gcggcaggaa tcagcccgtt catttgcgcc
    10861 aggtcgacgc cggcttcggt gtgtccggcg cggacgagta cgccgccttt ttgggcgcga
    10921 agcggaaaga tatgaccggg ttggacgatg tcttcgggtt tggcggtcgg ggaaacggcg
    10981 gtttgaatag tcagggcgcg gtcggcggcg gaaatgccgg tggtaatgcc gtgtgcggct
    11041 tcgatggaga cggtaaagtt ggtgccgtat tgcgcgccgt ttttttgggt catcatcggc
    11101 agcccgagtt tttcgaccat ttcgccgtcc atcggcaggc agaccaagcc gcgcgcgtgt
    11161 ttgatcatga agttgatggc ttcgggcgtg acgaattgcg ccgccatcag caggtcgcct
    11221 tcgttttctc ggtcttcggc atcggtgatg atgaccattt tgccggcttt gatgtcggct
    11281 aggatttcgg ggatggggga aatatgggac atggtgtgtt ttcctgtgtg ttcgggcggg
    11341 cgggcggtgt gttcggtatc gagccagaag gcgggcattt ttgccgcctg ttcgatttta
    11401 cgggcttttt tctcgccgaa ggatttgttt ttcaaaaggg cggagagttc gcctgtgttg
    11461 agggcaacgg cttcggcaaa acgggtttgc aaaccgccgt agtgttctgc tatccattgc
    11521 cgcaggttgc ggcggcgcag accggctgtg tcggtcatcg tttcatcctt tgtagaaatc
    11581 ggatgtagcg gattgtaatc gcgtatttac caaaaagcaa atctgttttt ttcgccaaac
    11641 cgaattattt gctttttggt aaatagatgc cgtctgaagc cggattcgtg gcttcagacg
    11701 gcattgccgc aatccccgcc ggcggggcgg ctgtttcttc ccgcccgctt ccgattcggg
    11761 atttgccgga agcttattcc gaaaaaaatt tttgataaac ggccttgatt gccgggtaca
    11821 gcataggcgg tacgagcagg cgcaatatcg gcaatcctat gatgaatgcg gtcttcccga
    11881 taaaaaccgg acggattgcc ttcgcgccga atatctttct gcatttttct ttaaatgaaa
    11941 aatatttccg gtagatgacg ataatgtttt tggaaaaata aagccttact aatatagacc
    12001 tcagatagcc gatattgtcg aagacaaatt tttgcaaacc gccgtagatg ggggaatttt
    12061 tgttttcgac taaaaaatcc acgccccggt cgagatgcgc cagcatatcg ctgaaatttt
    12121 tgtatttgat gttgtggctg atagagttgc cgagaacgcg gtactggtaa aaagggttat
    12181 cgtaaaaggc aaaagtcttg ataaaacgcg ccaattgcaa actgtacggg aaatcctcgt
    12241 gaatgtatcc ttttgggaaa aacagattgt ttttgatgat tatttcccgc ctgacaatct
    12301 ttgtccacgc gttggcgata tagtaccgcc cctccaccaa cgtttcaaaa tggcggacaa
    12361 aatcattatc cgaaaagtcc gcccctttgg ggatgacgtg gtaattgagg gacgagggat
    12421 gcaggatcaa atcaaccttt ttgtctgca
//
LOCUS       DAXIEB010000059        12299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000059 DAXIEB010000000
VERSION     DAXIEB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.059"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..203)
                     /locus_tag="ACFOA3_001289"
     CDS             complement(<1..203)
                     /locus_tag="ACFOA3_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6898293.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKP"
     gene            complement(375..557)
                     /locus_tag="ACFOA3_001290"
     CDS             complement(375..557)
                     /locus_tag="ACFOA3_001290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898294.1"
                     /translation="MIAISFLIAAVLITTLWRDKDSVAIEIVKIGGAVALGYFAGINR
                     GKAQTLEKQRREKDSD"
     gene            complement(563..790)
                     /locus_tag="ACFOA3_001291"
     CDS             complement(563..790)
                     /locus_tag="ACFOA3_001291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898295.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEILKRYTNAV"
     gene            complement(787..978)
                     /locus_tag="ACFOA3_001292"
     CDS             complement(787..978)
                     /locus_tag="ACFOA3_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898296.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(1022..1264)
                     /locus_tag="ACFOA3_001293"
     CDS             complement(1022..1264)
                     /locus_tag="ACFOA3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898297.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1500..1667
                     /locus_tag="ACFOA3_001294"
     CDS             1500..1667
                     /locus_tag="ACFOA3_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898298.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1940..2125
                     /locus_tag="ACFOA3_001295"
     CDS             1940..2125
                     /locus_tag="ACFOA3_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898299.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2326..2526
                     /locus_tag="ACFOA3_001296"
     CDS             2326..2526
                     /locus_tag="ACFOA3_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898300.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2676..3266
                     /locus_tag="ACFOA3_001297"
     CDS             2676..3266
                     /locus_tag="ACFOA3_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFC6898301.1"
                     /translation="MNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSKWIQRRI
                     KEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERNDKGRQAR
                     RYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIGKLLDMN
                     PGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            3270..3485
                     /locus_tag="ACFOA3_001298"
     CDS             3270..3485
                     /locus_tag="ACFOA3_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898302.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3646..4164)
                     /locus_tag="ACFOA3_001299"
     CDS             complement(3646..4164)
                     /locus_tag="ACFOA3_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC6898303.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(4274..4441)
                     /locus_tag="ACFOA3_001300"
     CDS             complement(4274..4441)
                     /locus_tag="ACFOA3_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898304.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4434..4607)
                     /locus_tag="ACFOA3_001301"
     CDS             complement(4434..4607)
                     /locus_tag="ACFOA3_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898305.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4716..4982)
                     /locus_tag="ACFOA3_001302"
     CDS             complement(4716..4982)
                     /locus_tag="ACFOA3_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898306.1"
                     /translation="MQVLKKVDWKMFVARSFWRSVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKIEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            complement(5072..5986)
                     /locus_tag="ACFOA3_001303"
     CDS             complement(5072..5986)
                     /locus_tag="ACFOA3_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6898307.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIDTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKVQAGIVKKYSSLIIR"
     gene            6057..6134
                     /locus_tag="ACFOA3_001304"
                     /pseudo
     CDS             6057..6134
                     /locus_tag="ACFOA3_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6201..8189)
                     /locus_tag="ACFOA3_001305"
     CDS             complement(6201..8189)
                     /locus_tag="ACFOA3_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6898308.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELTEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSA
                     PKLAHSYRRFVQWAEAENMPVNDVPSLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVN
                     VFDMDGKFITKAQTNGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALE
                     QAAVWDELGHLGGNVIEAEYAVLPRTDTDDFVLFEADR"
     gene            complement(8200..8388)
                     /locus_tag="ACFOA3_001306"
     CDS             complement(8200..8388)
                     /locus_tag="ACFOA3_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6898309.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            8508..8870
                     /locus_tag="ACFOA3_001307"
     CDS             8508..8870
                     /locus_tag="ACFOA3_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6898310.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
     gene            9047..9586
                     /locus_tag="ACFOA3_001308"
     CDS             9047..9586
                     /locus_tag="ACFOA3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6898311.1"
                     /translation="MLTGKGLPYLDKARLENAGAFSVSRNSDGITDTMGNPVDLSEFV
                     FVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWIDNYVTKQPEKLSVITVKGDSMEGVL
                     NHGDSILINHAKTDPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDFSN
                     AQDDIAIIGRVEWYGRSID"
     gene            9795..10385
                     /locus_tag="ACFOA3_001309"
                     /pseudo
     CDS             9795..10385
                     /locus_tag="ACFOA3_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            10490..11158
                     /gene="hda"
                     /locus_tag="ACFOA3_001310"
     CDS             10490..11158
                     /gene="hda"
                     /locus_tag="ACFOA3_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6898312.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            11155..11823
                     /locus_tag="ACFOA3_001311"
     CDS             11155..11823
                     /locus_tag="ACFOA3_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6898313.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3218 a         3314 c         2591 g         3176 t
ORIGIN      
        1 ggtttaccgt gtgggttacc ttttgcttta ggcttaaatc cgtgcgaccg catatcagcg
       61 acaacagact caagttcgga aacatccata tccgcacacg accgcttgcc cgtcacgcgc
      121 tccaacaccg cgcgataggt accgtcgtcc aagcccagct ccttttgggc aattttaatt
      181 tttgcaatca acgcccggcg cattatctct ccaatacaac atatagtata aattagcgca
      241 tattatacca ataaaatata atatatagta ttaagccgat gttttttgcg aaacggacag
      301 acatgaaaaa ggccgtctga aaacgagctt tgcgagtttc gccaaaacgt ttcagacgac
      361 ctttttacta caatctaatc actgtctttt tcgcgccgtt gcttttccaa ggtttgcgct
      421 ttacctctgt tgattcctgc aaaatatccc aaagctaccg ccccgccgat tttgacaatt
      481 tcaatagcga cgctgtcctt atcgcgccat aacgtagtaa tcagcacggc ggcaatcaga
      541 aaagaaatag caatcacggc attcagacgg cgtttgtgta cctctttaaa atctcaccat
      601 gttgctgttc accctgtttt tgcgcttcga tggtagccag tgcaatgcgt tcatgagatt
      661 ggatttcgtc cttacggctt tccaactcct tctgtttgac tgcaatatct tgtttttgaa
      721 gttctaaaaa ctcctgaagc attttctgct gctcatcggc cggcagattc tcggattttt
      781 ccccgctcat aaatcaaccg ccactaaggt tttgtaacgg actttgaatt tgccgtaacc
      841 ccgagcccct attttcggcg gcacaaattc cacggacgca atattgcccg tcttacggtc
      901 tttacggact tcttccaaat aatcggcagc attgagcagc cgttcgcgga ctgccgtata
      961 ttctttgacg gtattcattt ttcaacctcg cccaaatagt gcaaatatta gcatatggga
     1021 tttatttttc aagaaaatca aatccatata aaacaaccgt ataatatttt aatactatat
     1081 atggtaaatt tcttctaaaa atacacacta aaataccata tcttgataac cgcaacgccg
     1141 cacaaatggc agggcattga tgcccgacaa caaaaaatgc cgtctgaaac agcattcgag
     1201 ctgttttcag acggcatttt tctttgattt tgttggggaa agggttgact tttaacgtgt
     1261 ccacgtttat aattcaattc atcggcgtag tgaggccgat acgggaaaag ccccaccgaa
     1321 gcggggctca actgggagaa agaagatgaa taaacgcact attcaaattc tcatcttggt
     1381 cgcagttttc atgttggcaa gtgctaatgc ctactaaaga ctgaccagct gggggcggtc
     1441 gccgctgtcc ccagtcccaa tcttacaaaa tacggtttaa aaaatcaagg agtttgccta
     1501 tggttgatga aaaactggca gaaaaccgaa agcgttacga gcagaaacgg gtgattaaga
     1561 aagtctcgtt caatgccgaa acggaaaaag aacttttgga atatgcccaa aatatcgatt
     1621 tttcccaatg ggttaaatca atcatcaaag aaaaaatcaa aaaatagctt gactaaattc
     1681 tagaaatact agataatacg cctatctgat aaagcaaagc cccgaagcgg caacttcggg
     1741 ggcttatcgg atagaaagga ggcttgtgga aatgacaaaa ctggcgaaag ttgttttttt
     1801 catcattctg cttttaagct gtagtccggc ttactaagca agttgattaa acacaagggg
     1861 tggcagaaca ccgcgcccct ttcgccaacc tttctgcaag ccattttact acaacgattt
     1921 taaaaatcaa ggattgccta tgcctgatga aaaaacgcta aaagcaaaac aagctgaata
     1981 tcagcgaaag tatgaccaaa agcggctgat gaaaacagtt tctttccatt tggaaagaga
     2041 gaaagagttg gtggatttta tgcaaaaaga aattcccgac ttttcaaact gggttaaatc
     2101 agtcatgaag gaaaaaatga aataagaaaa ttccgccgat gggaaaacat cggcggaaca
     2161 ggtggaaagc agagcagcaa ctctctttat gtagggcatg aaaaaaggcc gtctgaaaca
     2221 ggttttaaac cccatttcag acggcttttt gttgtaatac atcttgatta aaatggtttt
     2281 tagtattacc ataatcgtgt tttaaaacct atttaaagga agattatgga ctcgattaaa
     2341 aaggctcaag aaaaatacgc actcaagcga gtagtgaaaa cagtatcgtt caattccgaa
     2401 aaagaacttg atttaattaa attttctgaa aatttagatt tttcaaactg ggtaaaatcg
     2461 aaaattagag gagatatgga atcggaaaaa ttcaaaaaaa aagcaaaaaa gaatattgac
     2521 aaatagaatt accataattt aaaatttcca tatcttgtgc aacacaagat atggaaatcc
     2581 ccgataagtg acagcttatc ggggatagtg gaaaagcaac gttagagcgt tcttttcctt
     2641 gtcatctcaa ccttgaaatg aaagataatt ttaccatgaa tacccaactc atcccaactg
     2701 tttcaggtca attggacaat caaacacaag cactggttga tgctcatgac ctgcataagt
     2761 tcttaggtgt ggaaacacca ttttctaaat ggattcagcg tcgcattaaa gaatacggat
     2821 ttacacaagc ccttgatttt atcggcgtgg acaaaattgt ccgcaccgaa gcaggtttct
     2881 ttggacagcg tgataaaact gttcaggggt attatttatc cctcgacatg gcaaaagagc
     2941 tgtgcatggt ggagcgcaac gacaaaggac gccaagcccg ccgatatttt atcgaaatgg
     3001 agaaacaggc aaaagccctg cctgatgccg tgctataccg catcgatgca ttggaagacg
     3061 cctatttcca agccgcgccc gaaatgctcg cgctgctgcg ctaccgcagt atgggcttga
     3121 acctgaccga aataggcaaa ctcttggaca tgaaccccgg cgcggtttcc taccgtctca
     3181 aaaaactcaa cgatttgggt ttcttggaat atgtccccaa gcccaaaatg gtggtgcaac
     3241 aacagtcttt gggattggag ggttaagcca tgaataccac ctataccctg actttcgacc
     3301 aagacagcct caaagccctg aatctgctgg tgttcacact caaccatctg aaagttttgg
     3361 agatggacat ggaaggaatc gaagacggtt tgtctgccgt gattgaaacc gcctccgaga
     3421 aagccgacaa gctgtccgcc gctttctaca acgccgtgta cgagcaggaa gagaaagcag
     3481 cctgaatcta gggcatgaaa aaaggccgtc tgaaacaggt tttaaacccc gtttcagacg
     3541 acctttaaat catgggttaa agcctatttg aaccggctag gcaagctcct gctccgtagg
     3601 ctcaatcacg tttcaacaca ctgaattgaa atcaggctgt tgattttata gaatttgttc
     3661 gctcggctca atcacaaaat cctccacgcc gctcacaatc ttaatccccg gcacttggcc
     3721 gtctgaaaaa cgctcttttt gattcaggat ggcgtctttg tcgatttcct ttttagtgcg
     3781 gacaaactcg gcaaaggcgg atttttccga gagccacgcc aagacggcgg ccacgcccgt
     3841 taccttgacg gatggcggac ggatgcgcca tttaatcagg ccggtagtaa agtccacggt
     3901 tttggtttta ccgttttccg tcagctcgtc cttatgtgcc tcgcagtatg cggccacacg
     3961 ttcggtcagg ctcatgattt cggcacacat cggcgcggct tgggcggcat attcttcttc
     4021 gatggccgct tttttgtctc cggcttcggt ttccaggcgt ttgacttcgc gctgcaagtc
     4081 gccgatttgg cggataaatg cagtaacttc cgttttgtct tgtgccgctt cgatggcggg
     4141 ctgtttgatt cgggttttag ccattttctc ttcctttcag gttgggttta acatttcggc
     4201 ttactcaagc cttgttcgcg ttcgtgcttg gcaagtgctt caactttcgc gcggtttctc
     4261 aaaaacttaa acatcacact tcctcccata aagttatcgc ctgagccaaa gtttccgcct
     4321 cggcagtctt ccacatcccg tccggcgcac gcgctgcaat cacaaagcct tcgccgtcct
     4381 tcttcatgac catcagctcg ccacggtctt cgagccattc gattaaatct ttttcattca
     4441 tctttgtttc cttccgtttt cagacgacct ttgccgtcct tatcttcaaa ctgcgcctga
     4501 tattcctcga tttcgcgctc ccggcttttt tgcgccattc gcgccgtcgc acgcctgcgg
     4561 tgttgtcccc aagcctgcca atccgtatta cgtcgtccga aactcatttc acacatcctt
     4621 tcacaatcgc cttatcgcca tatttcgcac ggatttcctt taccgcccga tccaaagcct
     4681 cttttttcgc cgcagggctc aataaaattg gtttatcact cataaacaat ccctttcatt
     4741 ttttcctcga cgctcattga ctcgtattgc tcttcccata ccttgacctc caaatctgcc
     4801 tgtctttcca tcttctcgat cttcgtcggc tctttcgcaa ccggttcggg ttctttcgcg
     4861 caggaatgca acgccattcc cgccacaaaa caccacacgc ccaccgtcaa accaaccggc
     4921 acactccgcc aaaaagagcg tgccacaaac atcttccaat caactttctt caaaacttgc
     4981 attttgcgtt ttcctttaga aaacagtaac ttattaaaat catagggtaa aaaatatata
     5041 gccctgtcag agacttaccg tttcagacgg cctatcggat aatcagggag ctgtatttct
     5101 taacgatacc ggcctgcacc ttgataccgt tcttgttcgc cgtgcgtacc gcgccgcgca
     5161 tcaatttgct catccggcgc gtattgccgt tactatgttt aaccagttcc aagagcgttt
     5221 cttcgtccgc atcaggcaaa gccgctttcg caatctcaaa gagttcgtca tccggcaaag
     5281 attcgcccaa attcagcgga agcgacacgc ggctgtaaag ctgtaccagc tcgccgtgct
     5341 tgccgcgcaa attcgcaacc agtcggggca taccgctcaa caccaaaccg cagccagtct
     5401 catcgtgcag tcggcggata atctcaaggg cgcgtaacgg caggttttcc gcttcatcga
     5461 ccacaatcag acggcccgaa tcacgcaggc ggtcagatac agactcaaac aaatcattca
     5521 ggctgcccat cgccgacacc ttcgccgccg tcgccaactt gcgcatcagg accaaagccg
     5581 taaagctcgg attagcctca atcaagatgg ccgcagggtt tttctcgcag tagtttttga
     5641 ccgcctgagt cttgcccaaa ccggcctgac cgtagatcac cactatttcg ccgccttcgt
     5701 gcgcatcgcg catcgcttcc gcgattcggc gtgtcgtctt agtcgataca aaacccaaca
     5761 ccagctcttc gcgtttcgct ttactgtcct gcatctccaa aaacgcctcg attttcggct
     5821 cgatggtttc ataattgccg cctttttccg cataagtgcc attcagatac atactgacgg
     5881 atgccggcga agtatcgata ccgcgtgcca gttgggtttg gctcataccc gatttttctt
     5941 taaattcagc cagtttttgt tgcaatgctt gattgatttg tttcattttt aatatccttg
     6001 agttttaaac aacctttaaa ggccgtctga aatgaaagag tttcccgatt tagaaatttg
     6061 agcctgattc cgttcagcgg gaacaccttg aatctttact tgaaatcgtt gaatccgccc
     6121 aagggcccgc ttgagtttca atttttcaac aagctcggtc gaaaacgccg catttttctg
     6181 tttgtccatc atgttttcct ttatctatcc gcctcaaata gcacaaaatc gtccgtgtcc
     6241 gttctcggca ataccgcata ctccgcctcg atgacgtttc cgcccaaatg tcccagctcg
     6301 tcccaaaccg cagcctgttc cagagccgga ttgacttccg cgtttgcgag cttgattgca
     6361 ttttccgccc gcttgatttt gccttttcgg cgtttttccg ccagttggtc gatacgcgcc
     6421 gtcgggaaag cctcgcgggt attgccgttg gtctgcgcct tagtaataaa cttgccgtcc
     6481 atatcaaaca cattgaccgc cgacgcatcg ctcaaatcgt agctgacccg tacctcgtcc
     6541 ttgtgatact ccgccagctc agttgagaaa taagagttgt tgaacaaatc cagccaaccg
     6601 cgctgtacct ttcgcacctc ttgcggcata aacatcgtcg ccagctcttc cgccgacaac
     6661 atatccggcg cgataccgtc ctgttccagc ctcatttccc gataagcctt cggcgaataa
     6721 tgcccgccgt ccggatgtcg gggcagctcg ccgtgcgggc ggttgttgta ttcgtcgata
     6781 cacttgacca catccgcgat aaaacgcgac cagcccggca gttttttcaa atatttctgt
     6841 tgttcctccg tcaaatcctt gcctttttcc aaagcgttaa acgcactttc catcttgcgg
     6901 tacatcaggt tcttggtact gctgtccatc cccgcgcccg caaacgtctc atactgtcgc
     6961 gccatctcaa tcagattgtc tttccaccat cgctcgatga tgcctcggcc ttgcgggttg
     7021 cccgcgatac ccgtttcatg ccggataccc aatcgggacg taatacccgt gatttcatgg
     7081 tctatcgtct tgccggtttg gccgccaccg ttatccgagt agtagataat cggcaaacca
     7141 aagtgcttga ccccgatacg cagagcgtcc gataccgcca cacaactttc agccaacgag
     7201 accgaaaacc cgaccacaaa ccgcgtacaa ccatcaataa tcaccgtcac ttccggctta
     7261 aacggcctgc catgtaccgg atgcgccacc tttgccttaa agctatggcc gtcgccgatc
     7321 caaacatcgt tcggcttcaa agccccccaa tcacgtttca cataaggcag cagcgattta
     7381 taagccgccc ccgttttcct gccgcgctcc tgcataatca gcgggagctt gtcccaaacg
     7441 cgccgcacca tactcaagct aggcacatca ttgaccggca tattttccgc ttccgcccac
     7501 tgcacaaacc ggcggtagct gtgtgccagt ttcggcgcgg acgggatatt gtgaaactgc
     7561 ataaacatcg gcaaccaacc gtagctctca atcggcttga ccgccttcgt cgtcttcgga
     7621 gccaaagcaa ccaaccgctc cgtcgcgttt tccgctttca aataagcaga aatccagccg
     7681 tctaaagtac gttcgccaac cttcgccgac cggctgcggt cattggcctt ttccaagttc
     7741 ccaagcgtga ccgcgtccaa tttactttct gccagcaagc ccaaaaactg agccaccgca
     7801 gcctttgcag agcaaccgta ttggtattta atccccaata ccgccgccac caccgcacat
     7861 cgcgcatccg ccaccgaccg ttgtttctcg ttcaacagct tggccgcttc agccagtgcc
     7921 tgagccgaca tcgccgtccc cggtctgact tggggcaagg ttttcggcat cttctccgtc
     7981 agctcgtccg actgccgttt catgatggct gctcggattt cggcagggag ggaagcgatt
     8041 aagtattttt tcagacggcc tccacgcgct ttgccaacct cttcgatgta cggccagttc
     8101 tgggtttttg ctctctccct taatcctctt gctgttgtag gaagcccttg aatattaagt
     8161 ttaagcagct caccaatcgt tatccccatc ttcgtttcct cactcgtcaa ttcttaaccc
     8221 cagcttaatc gcgatagcgc gcccctgacc gtatcgacac tttgatttcc ccgtcaacac
     8281 acgagtaaca tctacggagt tgtgtccgtt ttctctcgcc cattgcgcaa ctgttaaatt
     8341 attgtctttt aaccattctc taacttcatt tactgtttta attcccattt ttttaccttt
     8401 cttggtttgt tcaaatcctg tataattact acaaaactag ctgttttgct acatttgtag
     8461 caatattatt agctacaaat gtagctgtca agaaaaaagg aacttttatg gataattctt
     8521 ttgaattaag ctctaagtta ttgggtgagc gactaaaaaa aatcagaaca gataatggat
     8581 actcaagaga gcagttgagt gagttatttg gcatcagtag ggcaagtatt caaaattatg
     8641 agaatgggga aagaagtccg aatgccgatt acttagttca gttttataag cactttggaa
     8701 ttaatttgca ttggctgcta actggaaata aggctgccaa attccaagat ttcattgatt
     8761 ctgtttcttc tccgagagaa gaagtactac tacacttggc tcgccaaatg gatagtcgca
     8821 ccttgaatca cttgattgac tttctaatgg atgtttatgc taaaaaataa acagcactaa
     8881 ggaaaactca gcttttaagt tccttcctta gtgaacagtc gcctaaatag agaaattttt
     8941 tttaaatttt gattttgaaa tgaaaaatac aaaggaaggc ttctaaacct cttcctttgt
     9001 attttccttt gtcttgcaag aatctacagg ctgagattta aactggctgc tgaccggcaa
     9061 aggcttaccg tatcttgata aagccaggct ggaaaacgct ggagcattct ctgtaagcag
     9121 aaattctgat ggaattactg acactatggg caaccctgtc gacttgtcag agtttgtatt
     9181 tgtgccgcgc tacaacgtag ccgcagcagc agggtatggc gcaccggtct tcggcgaaga
     9241 gcctttgttc tgcttggctt tccggaaata ttggattgac aactatgtga ccaaacagcc
     9301 ggaaaaactg tctgtaatta ccgttaaagg cgattcaatg gagggcgtgc tcaatcatgg
     9361 ggattccatt ctcatcaatc acgccaaaac cgacccgcgt gacggcctat atgtcttgcg
     9421 tataggcaat gacctctttg tcaaacgtgt gcaacgcata ccgggcaaac tgcttgtaac
     9481 gtctgaaaat ccacgttacg caccgtttga aatagatttt agcaatgccc aagacgacat
     9541 cgccatcata ggccgtgttg agtggtatgg ccggagtatt gattaaatcc attttaaaaa
     9601 cctcttaaaa cagttttaaa aattccctaa tctcgtcagc tttaaacaga aaaccgcgca
     9661 ttccggcgcg gttttgtgaa aaagctggcg taacttttcc ggatacaaaa aaacgccgaa
     9721 atccacgtct ttccgagatt tcagcgtttt ttactctatt tgttccttgt gcaaaaacta
     9781 acagtccccc acaggaaaac gtcgctctgt tccgccacaa cggcgaaatc ctgttgagcg
     9841 gcatcaacat caaactcaaa agcggcgatt ccctgctgat acgcggcccg agcggttgcg
     9901 gcaaaacttc gttgctgcgc gcgctggctg ggctttggcc gttcggcagc agcggcaaag
     9961 tcagccgtcc gccgcatcaa gacatcctct tcctgccgca acgcccgtac acggcacagg
    10021 gcagcctgcg cgacgcggtt tgttatcccg acatcgacaa acagcatccg gagctaatcg
    10081 aagccatgaa cacctgccgc ttgggttatc tggttgataa attagataaa accgacgact
    10141 ggcaacacaa actctccccg ggcgaactgc aacgcgtcgc cttcgtccgc gccctgcttt
    10201 ccaagcccaa aatcgtcctg ctcgacgaag ccaccgccgc cttggacgaa ccggccgaag
    10261 ccgcgctcta ccgcgcctta aagcaaaaac tgcccgacag catcatcatc agcatcggac
    10321 accgaagcac gctcaacgcg ttccacaata tgcgcctcga tgtcggcaac gttgcctgcg
    10381 gttgaacgat gccgtctgaa gccgttttca gacggcatcg ttcaaattgg aaaggcgcgc
    10441 gaaaacaacg tacaataaac ccgtttacca cccctcggaa tgccgaaccg tgaaccagct
    10501 tattttcgac tttgccgcac acgactatcc gagtttcgac aaattcctcg gcacggaaaa
    10561 cgcggaactg gtctatgtcc tccgacacaa acacggacag tttatctatg tctggggtga
    10621 agaaggcgcg ggcaaaagcc atcttttgca ggcgtgggtc gcacaggcgc tcgaagccgg
    10681 caaaaacgcc gcctatatcg atgccgcctc catgccgctg accgatgccg cgttcgaggc
    10741 ggaatacctc gccgtcgatc aagtcgaaaa actgggcaac gaagaacaag ccctgctttt
    10801 ttccatcttc aaccgcttcc gcaacagcgg caaaggcttt ttgctgctcg gttcggaata
    10861 cacgccccag cagcttgtca tccgcgaaga tttgcggacg cgtatggcgt actgcctcgt
    10921 ttacgaagtc aaacccttaa ccgaccaaga aaaaatcgac gcgctcgcca gtatggcggc
    10981 ggcacgccaa gtaaccgtcg attccgaaat tttcgaatac ctgctcaaac actggcggcg
    11041 cgatatggac agcctgatga tgatgctcga cacgctggac aactacgccg tcacaatggg
    11101 caaacgcatc accctgccgc ttttgcgcca gcttttgaaa caacaggaaa cccaatgaaa
    11161 aacctcgcca tcttcgacct cgacaacacc ctcatcaaca ccgattccga ccacgcctgg
    11221 ccgcaatacc tcatcaaaaa aggcttggtt gacgctgccg aaaccgaagc gcaaaacgaa
    11281 aaattctacc gcgactacca aaacggctgc ctcgacatcg acgcattcct caaattccac
    11341 ctcgccccgc tcgcccgtta cagcaaagaa gagctggcgg aatttcaccg cgaatttatg
    11401 gcggaataca tcatccccca catctcgcct atgcagcgta tgctggtgca gagccaccaa
    11461 atggcaggcg acgaaaccct cgtcatctca tcgaccaacg agttcatcat cacccccgtc
    11521 tgccgccttt tcggcattac caacatcatc ggcacacaac tcgaatccgg ttccgacggc
    11581 cgctacaccg gcaattacat cggcacgccc agcctcaaag aaggtaaaat cacccgcctg
    11641 aaccaatggc tcgccgaacg cggcgaaacg ctcgaaagct acggcaaaac ctatttttac
    11701 agcgactcca aaaacgacct gccgctgctg cgcctcgtcg acgaacccgt cgccgtcaac
    11761 cccgatgccg agctggaaaa agaagccaaa gaaaaaggct ggccggtttt gaatttcaaa
    11821 tgacgcaatg ccgtctgaag cccgattcgg cgttcagacg gcattgaggc tgaaatccaa
    11881 aaaactcaaa gccacaggaa tttatccgga aaaacaaaaa acacaggaat tcatcgggaa
    11941 aaacaacaat ctttccaccg tcattcccac gaaagtggga atcttgatcc gtccgcacgg
    12001 aaacttatat ttcgtcattt gcacccgaca taattcaata ctttattttg caataaatta
    12061 acggatgctg acaaattaga ttggattaat taccctatta tccatattgg gttatcgggt
    12121 tgctaattga gcagagcgta gcaaaccata aagtgagtgt ttccacccac tctgcattta
    12181 tagtggatta aaatttatag tggattaaat ttaaatcagg acaaggcgac aaagccgcag
    12241 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttaaatt taatccact
//
LOCUS       DAXIEB010000060        12218 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000060 DAXIEB010000000
VERSION     DAXIEB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.060"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(168..590)
                     /locus_tag="ACFOA3_001312"
     CDS             complement(168..590)
                     /locus_tag="ACFOA3_001312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6898314.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(601..1998)
                     /gene="atpD"
                     /locus_tag="ACFOA3_001313"
     CDS             complement(601..1998)
                     /gene="atpD"
                     /locus_tag="ACFOA3_001313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6898315.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2036..2911)
                     /gene="atpG"
                     /locus_tag="ACFOA3_001314"
     CDS             complement(2036..2911)
                     /gene="atpG"
                     /locus_tag="ACFOA3_001314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6898316.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2936..4483)
                     /gene="atpA"
                     /locus_tag="ACFOA3_001315"
     CDS             complement(2936..4483)
                     /gene="atpA"
                     /locus_tag="ACFOA3_001315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6898317.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVLVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4494..5027)
                     /locus_tag="ACFOA3_001316"
     CDS             complement(4494..5027)
                     /locus_tag="ACFOA3_001316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6898318.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5032..5502)
                     /locus_tag="ACFOA3_001317"
     CDS             complement(5032..5502)
                     /locus_tag="ACFOA3_001317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6898319.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5573..5809)
                     /gene="atpE"
                     /locus_tag="ACFOA3_001318"
     CDS             complement(5573..5809)
                     /gene="atpE"
                     /locus_tag="ACFOA3_001318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6898320.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5866..6732)
                     /gene="atpB"
                     /locus_tag="ACFOA3_001319"
     CDS             complement(5866..6732)
                     /gene="atpB"
                     /locus_tag="ACFOA3_001319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6898321.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6722..7075)
                     /locus_tag="ACFOA3_001320"
     CDS             complement(6722..7075)
                     /locus_tag="ACFOA3_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6898322.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(7443..7781)
                     /locus_tag="ACFOA3_001321"
     CDS             complement(7443..7781)
                     /locus_tag="ACFOA3_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898323.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGSC"
     gene            complement(7916..8776)
                     /locus_tag="ACFOA3_001322"
     CDS             complement(7916..8776)
                     /locus_tag="ACFOA3_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6898324.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8934..9503
                     /locus_tag="ACFOA3_001323"
     CDS             8934..9503
                     /locus_tag="ACFOA3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6898325.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(9565..10428)
                     /locus_tag="ACFOA3_001324"
     CDS             complement(9565..10428)
                     /locus_tag="ACFOA3_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6898326.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPATWNEGAAK"
     gene            complement(10587..11273)
                     /locus_tag="ACFOA3_001325"
     CDS             complement(10587..11273)
                     /locus_tag="ACFOA3_001325"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6898327.1"
                     /translation="MADLTFQQAVSTIVGMKDEIFRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKLVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            complement(11275..12012)
                     /locus_tag="ACFOA3_001326"
     CDS             complement(11275..12012)
                     /locus_tag="ACFOA3_001326"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6898328.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAD
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
BASE COUNT         2945 a         3153 c         3114 g         3006 t
ORIGIN      
        1 tgatttttgt taatccgcta tatgtttcgg ttaggcggca ggctgcccta ttgaatacct
       61 taaagcaggc tatgcctgcc aacgccatat ccaaacacag tctttaattt aaatccggaa
      121 aataaaaagc acgaccaaac ggtcgtgctt ttccaaacca aacaagttta tttcttgtgc
      181 gaacggatat agtccaaagt tttaagctgt gcaatcgcag cagccaatgc tttatgcgcc
      241 tccgccaaag ccttatcgtc tttagcttgg gaaatgcccg cttctgcggc ttttttcgcc
      301 tcttccgcac gtgcctgatc catctctgca ctgcggacgg caacatccgc caagacagtt
      361 actttatcag gctgtacttc caaaacaccg ccggaaacag caaccaaaac ctctttatcc
      421 tcgcccggaa cggtcaaacg caaagccccc ggccgcacca aactcataat cggctcgtgt
      481 cgcggataaa taccgagttc gccctgtaca gtcggaacaa cgataaatgt tgcctcgcct
      541 gaatagattt tctgctcgct acttaccacc tcaacttgca tgatgctcat gccgacctcc
      601 tcagtttaag gttttcgctt tctcaaccgc ttcttcaatg ctgccgacca tatagaatgc
      661 ctgctcgggc agatgatcgt attcgccgtt caagatggct ttgaagccgg caatggtatc
      721 gcgcagggcg acatatttac ccggagaacc tgtaaacact tcggcaacgt ggaacggttg
      781 ggacaggaag cgttggattt tacgcgcacg cattacggtc agtttgtctt cgtcagacaa
      841 ttcgtccata cccaagatgg cgatgatgtc gcgcaattct ttgtatttct gcagggtgga
      901 ctgtacgccg cgcgccacgt cgtagtgctc ttgacccaag accatcggat ccagttggcg
      961 cgaagtggaa tcaagcggat cgactgccgg gtaaataccc aaagaggcaa tatcgcggct
     1021 caatacgacg gttgcgtcca agtgggcgaa cgttgttgcc ggagacgggt cggtcaagtc
     1081 gtccgcaggt acatatacgg cttggataga agtaatagaa ccggtttggg tagaggtaat
     1141 acgctcctgc aaacgaccca tttcttctgc caatgtcggt tggtagccca ctgcagacgg
     1201 catacggccc aacaatgcgg atacttcggt accagctaga gtgtaacggt agatgttgtc
     1261 cacgaagaac aatacgtcgc ggcctttgcc gttttcgtct ttttcgtcac ggaagtattc
     1321 cgccatggtc aaaccggtca atgcgacgcg caaacggttg cccggaggtt cgttcatctg
     1381 accgtaaacc attgccactt tatccaatac gttggaatct ttcatctcgt ggtagaagtc
     1441 gttaccttcg cgggtacgct cacccacgcc tgcgaacacg gacaagccgc tgtgcgcttt
     1501 ggcgatgttg ttgatcaatt ccatcatgtt cacggtttta cccacaccgg caccgccgaa
     1561 cagacctact ttaccgcctt tggcaaacgg acacagcaag tcaatcactt taatgcccgt
     1621 ttcgagcaat tcggttgtgg aagacagttc gtcaaactta ggagcggctt ggtggatggc
     1681 acgactcttg tcggtatcga tcggacctgc ctcgtcaaca ggcgtaccca atacgtcgac
     1741 aatacgaccc aatgtacctt tacctaccgg cacagtaatg ggcgcaccgg tattgctcac
     1801 agtcatgccg cgtttcaaac cgtccgagct gcccatcgca atggtacgga ctacgccgtc
     1861 gcccaaaagc tgttggactt ccaaagtcag accgttttcg tctaatttca aagcgtcgta
     1921 aacgcgcgga atcatgtcgc gtggaaattc cacgtcaaca accgcaccga taatttgtac
     1981 gattttgcct tggctcatta tcgtatccta atttctgtac aggattcaga cggcatcaga
     2041 cagccgccgc acctgctaca atttctgaca attccgtggt aatcgcagct tgacgcgatt
     2101 tgttatatac caaacgcaac tctttgatgg cattgcctgc attgtctgtt gcagctttca
     2161 tggcaaccat gcgggctgcc tgttcggatg ccatattgtc gctcaacgcc tgataaacca
     2221 cagactctaa atagcggcga accagatatt ccaacactgc aagtgcagtc ggttcgtagc
     2281 ggtattccca gctgaacggt gatttgggag ctgaatcgcc aatcacgttc tcaccgatag
     2341 gcagcaatac ttccattctc ggttcttgac gcatggtatt gacaaaaccc gaatacacca
     2401 gatggattct gtcaatttca tgtttctcat accgttggaa gagttctgtc aaaggtccga
     2461 gcagcatttc catttttggg gtatcgccca aatttacggc actggcaacc acattcagac
     2521 caatgctctg acacgccatc agacctttac tgccaaggca tacgatttcc tcttcaatac
     2581 cttgattccg atactcttga acttgtgcca aaaacttttt cagcacgttg gcgttcaaac
     2641 cgccacacaa acccttatca gacgtaatca aaataaaacc gacacgtctg atttcccgat
     2701 gagattccag taacggaata ccatgatcgg tattggtttg cgcaagatgg ctcatcacca
     2761 tacgcacttt ttcggcatac ggacgcgcca aacgcatccg ttcctgagtc ttccgcattt
     2821 tagaggttga caccatctgc atcgctttag tgatcttttg ggtattctga acactgcgga
     2881 ttttggtgag aatctctttt cctactgcca tttcagactc ctttcacttc aagccttatg
     2941 cctgataggc gtaagaagat ttgaaggatt tcatggctgc ttcaagcgtt ttctcgctct
     3001 cgtcggacat tgcacctgaa gcattgacgg cttccaaaac ttccggatgt tgggtacgga
     3061 caaagctcaa aaattcagat tcaaaagcca gagctttggc aaccagaaca tcagaatacg
     3121 aaccgttgtt gattgcccaa agggtcaaag ccatttcagc cgtattcaac gtactgaact
     3181 gtttctgttt catcagttcg gttacgactt cgccatgctc caattgtttg cgcgtagctt
     3241 catccaaatc ggatgcaaat tgcgagaacg ccgccaattc acgatattgt gccaacgcca
     3301 aacggatacc gccacccagc tttttaatca ctttggtttg tgcagcaccg cctacgcggg
     3361 atacggaaat accggcattg attgcaggac ggataccggc gttgaagagg tcggtttcca
     3421 agaaaatctg accgtcggta atcgaaatga cgttagtcgg aacgaaagca gatacgtcgc
     3481 ccgcttgggt ttcgataatc ggcaacgcgg tcagagaacc ggttttgcct tttacttcgc
     3541 cgttggtcaa tttctccact tcgtgttcat tgacacgtgc cgcacgttcc aacagacggg
     3601 agtgcaggta gaacacatcg ccgggatagg cttcgcggcc gggcggacgg cgcaaaagca
     3661 gggaaatttg acggtaagcc acagcctgtt tggacaaatc gtcataaaca atcaaggcat
     3721 cttcgccgcg gtcgcggaag aattcaccca tcgtacaacc ggagtaaggt gcgatatatt
     3781 gcaatgccgc cgcttcagat gcagttgcag caaccacaat cgtgtgttcc atcgcgccat
     3841 gctcttccaa tttgcggacc acgttggcaa tagaagatgc tttttgaccg atagcgacat
     3901 aaatacagat aacgcccgta cctttttggt tgacgatggc atccaatgct acggccgttt
     3961 tacctgtctg acggtcgcca ataatcaact cacgctgacc gcgaccgaca ggaaccatag
     4021 agtcaatcgc cttcagaccg gtttgcatcg gctggtcaac cgatttgcgc gcaatcacgc
     4081 ccggtgcgat tttttcgata ggggcggtca aagttgtatt aatcgggcct ttgccgtcga
     4141 taggccgacc caatgcatca acgacgcgtc cgaccagttc gcgtccgacc ggcacttcca
     4201 agatacgacc ggtacaggta accgtgtcgc cttctttaat atgttcgtac tcgcccaaca
     4261 ctacggcgcc gacggagtcg cgctccaggt tcatcgccaa gccgaaagtg ttacccggga
     4321 attcgagcat ctcaccttgc attgcatctg acaaaccatg gatgcgaacg ataccgtcag
     4381 ttaccgaaat caccgtacca cgggtacgca cttcggcatt tacagacaga ttttcgatct
     4441 tggctttaat caaatcgcta atttcagcag gattaagctg catgaaaact ctcctaattc
     4501 gtcatagtcg tgtacaaggc actcaatttg ccttgtacag acaaatccaa aacctgatca
     4561 cccacttcaa cttttatgcc gccaatcagc tccggttcga tttcgacaga gattttcagc
     4621 tcgctgtcga aacgcttatt cagcatttgc gccaactcgc cgacctgttt gtcggtcaac
     4681 ggataggcac tgtaaatgac ggcagatttg atatggttga atgataaggt caagtcttga
     4741 tattgagcat atacttccgg caatatcgac aaacgtttct gcccggccaa gacgataaca
     4801 aagtttttca actccttgtc tttcaaaccg accaaatcga tgaggatatc tgctttttct
     4861 gaagcattcg tttcaggacg gtcaatcaat gaagccacct tcccttcctg aacaaccgcc
     4921 gcaagttttt ccagtccgcc caaccaagac tcaatttggc ttttttcctg agccagaccg
     4981 aacaatgcct ttgcataagg tctggcaatc gttgcgaact ctgccataag attacagctc
     5041 ctgtttcagg gtatcgagca gttttgcgtg tttggaagca tcgacttcgc tgcgcaaaat
     5101 agattcggca cctttgacag ccaacacggc aacctgctcg cgcagggatt cgcgtgcgcg
     5161 gaacaattcc tgctccacat cggcctttgc ctgagctgca atgcgcgccg cctcggaaga
     5221 agcctgttct ttggcttctt cgacaatttt ggcggcacgt ttttcggcgt tggcaaccat
     5281 ttcggaaacc tgattacgcc cttctgccaa gagttctgca accttttttt cagcctgctc
     5341 gaaatcgctt ttaccacgct cggcggcagc caagccttcg gcgacttttg cggcacgctc
     5401 atccaaagct tttgcaatcg gcggccacac gaatttcatg gtaaaccata ccaaaccgaa
     5461 aaagacgatg atttgagcga ataatgttgc attgatattc acgttactta accttcgtac
     5521 tggggttaat caaacaggct gcgcctgtac ggaacggacg aatccgtcct gattatgcac
     5581 ctgcaaacgg gttaacaaag gcgaacagca gtgcaatggc gacaccaatc aagaatgcgg
     5641 catcaatcaa accggcaatc aggaacagtt tggtttgcag cggaccgatc agttcgggct
     5701 gacgggcaga agactccaaa tatttagaac cgaccattgc gataccgata gaggcaccca
     5761 atgcacccaa tgcaacgatc aaaccacatg cgatagcaat caaacccatt ttaaactcct
     5821 taaagaaaca aaggttaaac tacaaaaaca aactacttag gaaaatcagt gcgcatcatg
     5881 tgcctgtccg atatagacga acgccaacgc catgaaaata aacgcctgca gggtaatcac
     5941 caaaatatgg aaaatcgccc atgccaaacc ggcaataatg tggaatacaa acagaatcgg
     6001 atccatgact tcgacgctgc cggaagccgc ccaagcaccg ccaagcaagg ctatcaacaa
     6061 gaataccaac tcgcctgcat acatattgcc gaacaaccgc ataccgtggg atacggtttt
     6121 agaaagaaac tcgaccaaat tcaacagaaa gttcgcaggt gcgagttttg caccgaacgg
     6181 cgcgctgaac aactcgtgaa accagccacc caatcctttg attttgatgt tgtaatagat
     6241 acaaatcagc aacacgccga cagcgagtgc caaagtggtg ttcaaatcgg cagtcggtac
     6301 gacgcgcagc agggcgtgat ggttgccggt aatgccctgc cataccatcg gcagcaaatc
     6361 gaccggcagc atatccatcg cgttcatcag aaaaatccag acaaacagcg tcagacccaa
     6421 cggcgcgacg gcttttctag acttttcgtt gtgaatgatg ctcttacaca tatcgtccac
     6481 aaactcaaac aagatttcca cggcagcctg gaaacgtccg ggaacgcctg ccgttgcttt
     6541 tttcgcaccg cgccacaaca ggaagctgcc gattacgccc aacaggacgg caaaaaagac
     6601 ggcatcaagg ttaataaacg aaaaatcagc aatgtttttc agtccctgac cctgagtaac
     6661 atccgacaaa ctggtcaagc tctgcaagtg gtgcttgatg tagtcggcag cggtaatggt
     6721 ttcacctgcc ataatctttc actctcaaca atactaaaaa aaccaaatgg ctgacaccga
     6781 gcagccccat cagaaacggg gcgaacacca acgattgatg ccatacagca aatacggcga
     6841 gcatggacaa cagcgacagc actactttta aaatctctcc gaagacgaac atcctgcttt
     6901 gcaggaaagg gtttcccctg aaaagtttta aaagtaaaac tgcaacaaac gtgggaagca
     6961 ggtaggacaa accgccaccg accgccgaaa ggaatccggc aaaaccccat acagcaaagg
     7021 caactgcggc gcatatggac aatacggcgg attgtaggat gataatctgc ttcataaagg
     7081 gaatgtttcc gcctcggatt tggggcgcgg ctaatataat ttagaagcct tattacgtca
     7141 agcgacagtt aatctttgtg aaacaacgta tcccaatccg ccgcgctcgc cgcctgaata
     7201 acggcgacag gtgtcattct aacacacatt acatataatt acaggatatt aaggagtttg
     7261 tccgcaattt ctttacactt ttaatgttct tacgtgattt gtttgcttta cgtggaaata
     7321 ataaaaaatc aacgcgaaat tgtagcagtt tatcggtcgg attgtcggca gtttggggaa
     7381 tttgctcaat aaataaaagg tcgtctgaaa atattttcag acgacctttt ccgaataaag
     7441 gattagcaac tgcctgccgc tttaagcaaa gcattgcatt gacttttgcc tttgtgcgtt
     7501 ccgcctccca aacaaattgc atcggaagtg gtaacgccga ttgtgctgat tacactggta
     7561 acatagcatt ggctcacgcg cttacccaca gttgcggtaa agttgatgcg tatgccttca
     7621 ttgttgcggt tgctgatttt tacggcattt gggctgacgc ccaaggcaaa cgcggcacgt
     7681 tcctgaagtt tctggtcgga aacggttaca ttattgattg agccgcaacc tgctaatgcc
     7741 aacgcaacga acgcagccga aacgatgatg cgtgtgttca taatttcctc gaaaattaaa
     7801 aatgaaaaca ggaaaacgat tcttacgtga agcagaaaaa tgtcaataga attatatttc
     7861 ccacttacaa tctggaaagc tattctctat atttcagacg gtatatcccg caaaattaag
     7921 gtcggtaatc tatgcccaac tgcttcaaga tatggtcgaa ggtttcgggc gtgtcgaaat
     7981 gcaggatgat tctgcctttt ttatggttgg cggttttgat ttcggcgttg acacccagtt
     8041 tttcagtcag caaatcattc aggcggccga tgtcggcggc ggcagttttt ttgggctcgg
     8101 gacgtttgtt ttgaagggcg gcttggctgc ggcgttcgac ttcgcgcacc gaccagccgt
     8161 ttttgacggc cttttgcgcc aactcaagct gttcgacgac gggcagggtc agcagtgcgc
     8221 gggcatgccc catttcgagg cggcgttggt aaagcatttc ctgcacgggt tcgggcaggc
     8281 ttaaaaggcg caggctgttg gaaatcgcgc ttcggctttt accgacggct tgggcgatgg
     8341 tttcgtgggt cagtccgaac tcgtcggcaa ggcgtttcaa gccttgtgct tcttcgatgg
     8401 ggttgaggtt ttcgcgctgg aggttttcga tcaaacccat tgccaacgcg gtttcgtcgc
     8461 tgatggtttt gataacggcg gggatttcgg tcaggccggc aatctgtgcg gcacgccaac
     8521 gccgctcccc cgcaatcagt tcgtatcggg acagtccgtg ttcgcgcacg atgacgggct
     8581 gtatcacgcc ttgcgcctta atcgaatctg ccagctcctg caaagcttcg tcatcgattt
     8641 gaacacgcgc ctgatagcgg ccgggccgga tatctttaac cgcaaccgtg gtcaatcggt
     8701 cgccgctgct gttgtctgcg ccgttggcga gcagcgaatc caaaccgcgc cccaatccgc
     8761 cttttacttt tgccataccg ccctcccgtg cctattcaga taggatgtta aatcggatat
     8821 tttatcggat attgggtgtt gccgacaatt tgtatccgta tttatcggat ttctgttttt
     8881 tcactataat agccggtttg ccgttgcagg cggttttatg ggaaaggcgg atgatggtac
     8941 ggcgtttgat aatcggcatc agcggggcga gcggtttcca atacggcgtg aaggctttgg
     9001 aacttttacg cgcgcaagat atcgaaacgc accttgtggt atcgaaaggt gcggagatgg
     9061 cgcgcgcttc ggaaacggct tatgcgagag atgaggtata tgccttggcg gacttcgtgc
     9121 atccgatcgg caatatcggg gcgtgcattg ccagcggtac gtttaaaacg gacgggatgc
     9181 tggtcgcccc ctgttcgatg cggacgcttg cctctgtcgc gcacggcttc ggcgacaacc
     9241 tcttgacgcg tgcggcggat gtggttttga aggaaaggcg gcggctggtg ctgatggtgc
     9301 gcgaaacgcc gctgaacctt gcccatttgg acaatatgaa gcgggtaacg gaaatgggcg
     9361 gcgtggtgtt tccccctgtt cctgcgatgt accgcaaacc gcagacggcg gacgacatag
     9421 tggcgcacag tgttgcacac gctttgtcgc tgttcggaat cgatacgccg gattcggcgg
     9481 aatggcaggg aatggcggat taaaggacaa aaatgccgtc tgaacacgga tacggttcag
     9541 acggcatcat tttatacgac tgccttattt ggctgcgcct tcattccatg tggcagggga
     9601 tttgtagccc tcgaagcgtt tgtgcgcgta ggctttgaac gcgtcggagt tataggcctc
     9661 ggttacgtct ttaagccatt ggctgtcttt gtcggcggtt ttgacggcag accagttgac
     9721 ataggcaaag ctcggttctt ggaacagggc ttcggtcagc ttcatgccgc tgcttatggc
     9781 gtagttgccg ttgacgacgg caaaatccac gtcggcgcgg ctacgcggca gttgcgcggc
     9841 ttcaagctcg acgattttga tgtttttcag gttctcggcg atgtccgctt tggatgcggt
     9901 cagcggattg atgccgtctt tgagtttgat ccaacccagt tcgtcgagca tcaccaagac
     9961 gcgggcgaag ttggacgggt cgttgggcgc ggatacggtg ctgccgtctt tgacttcttc
    10021 cagcgatttc agcttgcccg ggtacagtcc caaaggcgcg gtcggcactt ggaagacttc
    10081 ggtgatgtcc agattgtgtt cttttttgaa gtcgtcaaga tagggtttgt gttggaagac
    10141 gttgatgtcc aactcgccct ccgccaatgc cagattcggg cgcacatagt cggtaaactc
    10201 gaccagtttg acggtgtagc cttttttctc cagctcggct tggatttgtt ctttgaccat
    10261 atcgccgaag tcgccgacgg tcgtgccgaa gacgatttct tttttcgccg cgccgttgtc
    10321 ggcggcggca gaagcggatg cggcgggcgc gctgtctttt tgaccgccgc aggcggcgag
    10381 gatgagcgcg agtgcggcgg cggaaagggt tttgaagaag gttttcatat tttctcctga
    10441 tgttgtggca gtttcaaaca aaaatgacgg gcagggagtc ctgccgtcca gattcggcgt
    10501 tcagacggca tttgccgcga acagggggat tttatagcat ttttcggata gcggtggggg
    10561 ttttggcgtt cagacggcat tcgggtttaa cgtttgtcga gtttccgcgc caacgtgttg
    10621 ccggtgcttt gaatcaggat gaccagcagc acgaggaggg cgacgatgaa gacgatgact
    10681 tcggtttggt agcggtagta gccgtagcgg atggcaaggt cgcccaagcc gccgccgcct
    10741 atcatgcctg ccgccgcgct gtatgacaaa agcccgatgg caagcacggt aatactggaa
    10801 accatgcccg cgcgcgcttc gttcaagagg actttgcaga cgatggcaac cggcggcgca
    10861 cccatcgcgg cggcggcttc aatcacgcct ttggggactt cgcgcaggtt ttgttccacc
    10921 agtcgggcaa aataaaacaa tcccgacacg ctcaacacca gcgaggcggc aaccgggccg
    10981 atggtgctgc cgacgatggc gcgtgtggcg ggtatcatcg caatcatcaa aatgacgaag
    11041 gggaaggcgc gcatgaggtt gacgaggttg tcgagcagga agttcaccag cttgttgtaa
    11101 tgcagttggc ggctggaggt tacgaagagc agcacgccca gcagcgtgcc gaagatgacg
    11161 gcgaatgtgg tggataagcc gaccatgacg aaggtttcgc ccaaggcgcg gaagatttcg
    11221 tctttcatgc cgacgatggt ggaaacggct tgttggaatg ttaagtctgc catatcagtc
    11281 ctcccgaatc agttcgcgcc cgatgtcgga tcgggcgtgg atttggttgc cgcgtacttc
    11341 gacgatttcg acgactttgc ctttatccaa cagggcggcg cggtcgcaca gtcgtctgat
    11401 aacgctcatt tcgtgggtta cgatgacgat ggttacgttg aatcgtttgt tgatgtcttc
    11461 caaacattcc aagacgctgc gcgtggtggc ggggtcgagg gcggaagtgg gttcgtcggc
    11521 gaggatgact tggggtttgg gcgcgagtgc gcgggcgatg ccgacacgct gtttctgccc
    11581 gcctgaaagc tgggcgggat agtggccggc gcgttcggtc agtccgacga tttcgagaca
    11641 ttcggcaacg cgcgctttga ttttttcaga cggccatccg gcgatttcca aaggaaaggc
    11701 aacattgtcg gcaacggtgc ggttgctcaa aagattaaac tgctgaaaca ccatgccgat
    11761 attctgccgc gcctgacgca atgcggcggc atcaagcgcg gtcagctctt gtccacagac
    11821 gttgaccttg cccgtgtcgg gacgttccaa caggttaatc agtcgcaaca gggtggattt
    11881 gcccgcgccg gaatagccca tcagcccgaa aatttcgccg tcgcggattt cgaggctggt
    11941 cggctcgaca gcggcaaaac gggtcttgtc gcgcgtttgg taatgcttgg aaaccttgtc
    12001 caaaataatc attgtctttc ccatacaaca aagcccgatg tcggacacaa cgggcgcgga
    12061 agataaagct gaaattgtcg gaacgcttta gctgttatgc ccgcaagctg tgtcaaatcg
    12121 gcaggttaat tttcgtagga tattatcggg aaagcgtttt tttgtcaata aggcaggaag
    12181 cgggcaacca tttcggacaa tatagtggat taacaaaa
//
LOCUS       DAXIEB010000061        12071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000061 DAXIEB010000000
VERSION     DAXIEB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.061"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFOA3_001327"
     CDS             complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFOA3_001327"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6898329.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFOA3_001328"
     CDS             complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFOA3_001328"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6898330.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFOA3_001329"
     CDS             complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFOA3_001329"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6898331.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPTSCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDTGQDSK"
     gene            complement(2757..3257)
                     /locus_tag="ACFOA3_001330"
     CDS             complement(2757..3257)
                     /locus_tag="ACFOA3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6898332.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFOA3_001331"
     CDS             complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFOA3_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6898333.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVKLL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTNGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGDKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5774..7114)
                     /gene="rseP"
                     /locus_tag="ACFOA3_001332"
     CDS             complement(5774..7114)
                     /gene="rseP"
                     /locus_tag="ACFOA3_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6898334.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7149..8384)
                     /gene="ispC"
                     /locus_tag="ACFOA3_001333"
     CDS             complement(7149..8384)
                     /gene="ispC"
                     /locus_tag="ACFOA3_001333"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6898335.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFHPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8389..9186)
                     /locus_tag="ACFOA3_001334"
     CDS             complement(8389..9186)
                     /locus_tag="ACFOA3_001334"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6898336.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9189..9935)
                     /locus_tag="ACFOA3_001335"
     CDS             complement(9189..9935)
                     /locus_tag="ACFOA3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6898337.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9991..10548)
                     /gene="frr"
                     /locus_tag="ACFOA3_001336"
     CDS             complement(9991..10548)
                     /gene="frr"
                     /locus_tag="ACFOA3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6898338.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10728..11894)
                     /locus_tag="ACFOA3_001337"
     CDS             complement(10728..11894)
                     /locus_tag="ACFOA3_001337"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6898339.1"
                     /translation="MLFHISASDGTTDMNTSQRNRLVSRWLNSYERYRYRRLIHAVRL
                     GGAVLFATATARLLHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGL
                     GVLWLNQHYFHGNLLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWF
                     DSGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTR
                     ERLEQNMVKMRQINARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTT
                     AAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAE
                     HLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11927..12002)
                     /locus_tag="ACFOA3_001338"
     tRNA            complement(11927..12002)
                     /locus_tag="ACFOA3_001338"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11967..11969),aa:Thr,seq:cgt)
BASE COUNT         2264 a         3082 c         3765 g         2960 t
ORIGIN      
        1 tttaaaacac cacttttgta gtgtcctgta atttcagcac agaatattca ataagacaat
       61 atgttctttt gaaaaatact tttggttttt tctccgaaag caggtcggtt caagctgcgg
      121 aaattgtttg caattcttta aaagcagcgg cggaggtcac aatgaaatgt ccgaataggg
      181 atgtggcggg cggcagaaat catcaatgct gccgactgcc atacttctga aatctacaaa
      241 atgatgcatc gatcaaacaa tataccgctt taaaaaaacc gatgccgtct gaaacgcttt
      301 cggggtttca gacggcatca aaagggtacg gtcagcggat gatgccgcgc gtcgattgtg
      361 tgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc ggcgcggcgg aggatgtctg
      421 ccttggcctc ttcaaacgga atgccgcgat ggtagagggt tttgtacacg tctttgacgg
      481 cggaaatctg ttctgcggta aaaccgttgc ggcgcatgcc ttcgctgttg agccccgccg
      541 gttcggcgcg gtagcccgat gccataaagt agggcggtac gtctttgtgt acgcctgcgg
      601 cgaaggcggt catagcgtag tcgccgatgt ggcagaattg gaaaaccagc gtgtagccgc
      661 ccaaaacgac gtagtcgccg atggtaacgt gtccggcaag cgaggcgttg ttggcgaaga
      721 tggtgtggtt gccgaccacg cagtcgtgcg cgaggtggca gtacgccata atccagttgt
      781 cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc taaattaaag gtggtgaatt
      841 cgcggatggt gttgccgttg ccgataatca gcttggtcgg ctcgtcgcgg tattttttgt
      901 cctgcgggat ttcgccgagg ctggcaaatt ggaaaatgcg gttgttttcg ccgatgctgg
      961 tgtggccgtt gatgacggcg tgcgggccga tttcggtatt cgcgccgatt tggacgttgg
     1021 ggccgatgac ggtgtacgcg ccgactttga cgccggagtc gagttcggct ttggggtcga
     1081 tgacggcggt cgggtggatg agggtcatgt ttttcctttc ctgtcgtgtt gccgcgaaga
     1141 tgcgcgacgg caacaggtcg tctgaaaact ttcagacgac ctttttctga acactcaaac
     1201 cacgcgtttg gcacacatga tgatggcttc gacggcgact tgcccgtcca ctttggcaac
     1261 ggcgttgaat ttgccgatgc cgcgccggct ggtcagcagc tcgacttcaa agacgagttg
     1321 gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct atgccggcga agaagaagaa
     1381 ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat
     1441 cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg gggaaatggc cttggaactg
     1501 gggttcgttt atggtgacgt ttttaatcgc ggtcagggtt ttcatcggct cgaaggcggt
     1561 gatgcggtcg agctggagaa acggatagcg gtgggggatg agtttttgga tgtctttggc
     1621 ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt gaataaagtc ggtttggtta
     1681 tttgctgtct tgaccggtat ctgaaagctg ctgctctagt gttttgagcc gtttgttcat
     1741 ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat tctttatggg tggacatcgg
     1801 gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg acgtgccgcc
     1861 gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc cgccgccgat
     1921 gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg cgatgacggt
     1981 gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt tggtgccgtt
     2041 gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc cgatttccac
     2101 gtcgtcgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat cgccggcaaa
     2161 agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga cgcgtctgcc
     2221 cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt cgcccagttt
     2281 gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga gcacggtgtt
     2341 tgcgccgatg tagacgttcg cgccgatttc gcagctggtg ggaacggtcg cgcccggttc
     2401 gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa acaggcgggc
     2461 gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc cttcaaatcc
     2521 gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga cttcggcttt
     2581 gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga gcgggcgcac
     2641 ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg tgatttggga
     2701 cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg aaagggtcag
     2761 cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt gacgtaaatc
     2821 acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat gacgcggttg
     2881 gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc ttcttcaaac
     2941 tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc ggcttgcgcc
     3001 ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccaa accttcgcgt
     3061 tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt tttttgaatc
     3121 ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc gattttttgg
     3181 aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc ggcaaacgcg
     3241 cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc cgtctgaagg
     3301 gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg aagcgttgga
     3361 tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc gggcctaaag
     3421 gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta aaggtggatt
     3481 tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg gaactgctgt
     3541 tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg ctcaggcgga
     3601 cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag ctcggcggag acgttggctt
     3661 ttttgttgcc gccgtagctg atcttgtcgc cgtattcgtc atagactttc ggaccgagtg
     3721 tgccgctttc gtaacctcgt accgaaccca ggccgccgcc gtagaagttt tcaaagaagg
     3781 ggatttcttt ggttctgccg tagccgcctg caatgccgac ttcgccgccg agcatcagcg
     3841 tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag tagtattgca
     3901 gtttgctgcc gggcagggcg atttcggcgt tcacgcccgt caggtagccg cgcgtcggcc
     3961 ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac agccagcctt
     4021 tgaagctgcc gtctgtgccg ttggttttgc cgtattgttt gataaagtcg gcatagcgtt
     4081 tgggtgcttt gttgtaggtg ttgacggtca ggtgttctgc cgccaaaccg aaattcacgc
     4141 ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg gtggttttat
     4201 attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa acatcgtagc
     4261 ccaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag ccgttgagcg
     4321 tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac aggttgtctt
     4381 gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg ctcaagtcga
     4441 gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg ggtgtgccgg
     4501 caagcgggac ggcgtcaaac tggacattgt cgaagtagcc taaaagcttg acgcgctctt
     4561 tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc aattcgcggc
     4621 gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg gatttcgttg acgtagattt
     4681 tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg tttggcagcg
     4741 gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc tgaatctcac
     4801 ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg gtcagcagct
     4861 tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact ttgccccaac
     4921 ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg tcttcgttgg
     4981 tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg tagaagtcgg
     5041 ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt cgtgtcagcc
     5101 atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg tcggaataga
     5161 cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg tcaatcgtga
     5221 tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt tggatattga
     5281 gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg agtgtcgcct
     5341 gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta atggcgtcgt
     5401 tttgcagcat ttttgcgccg gtgatattga gcgagccgat ggtaggacgt tcgatgacgg
     5461 tcagcaggag ctgcccgtcc gcagtttcga cgcgtacgtc gtcaaagaaa ccggtggcgt
     5521 acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg actttgacgg
     5581 gcaggtagtt gaatacggtg ctcggctcgg tacgctgcaa gccttcgacg cggatgtctt
     5641 ggatggtgaa gtcggcaaat gccaaaggcg atatgcccaa catcatcagt gcggaagcaa
     5701 tctgtttcag tttcatcgta agttccttag tggtgcggaa tgcggtttca gacggcattc
     5761 cgaaacgtaa aatctaaccg agcagccggg taacgtcgtt gaagaaggcg accgccatca
     5821 tcagcatcat gagggcaagc ccgaagcgca aaccgatgtt ttggacgcgt tcgcccaaag
     5881 gtttgccgcg tatccattcg gcagtataaa acacgaggtg gccgccgtcc aaaacgggga
     5941 cgggcagcag gttcagcacg ccgaggctga tgctgaccag tgccaaaaat tccaaataac
     6001 tttgcaagcc gagttcggcg gactgtccgg caatatcggc aatggtcagc ggaccggaaa
     6061 tatggctgac ggaggcgttg ccgctgatta gtttgccgaa aaatttgagg gttgtccacg
     6121 agtgggaaac ggttttttcc cagcccatgc cgaatgcgcg gacaacagac ggacggtagc
     6181 tgcggcggat ttgcgcgtcc cacgccctgt ccggctgcgg acggaggccg acgcgcccga
     6241 tcagggtatg gtcgggctgt tcgacagtat cggggcggat gtcggcggta tgggtttgtc
     6301 cggcgcgttc gtaggttaag gcaattttcc tgcccgggct ttggcgggtc aggtttgccc
     6361 attcttgcca tgaggcgatg ggtttgccgt cggcggcagt cagcctgtcg cccggtttca
     6421 ggcctgcttt ttcggcgggg ctgccttttt ccacgccgcc ggcaacggtt gtgattttaa
     6481 agggcatcag tccgatgtag ccttggtttt tggcgatttt accggcttct ggcgtgcctg
     6541 cggcatcgat ggtgcggacg gtttgcgcgc ccgatgccgt ctgaacgccg acggcgactt
     6601 tgccggcttc gaggttgagg acgatttcgg tttgcgcgct gccccaatct gcaacgggtg
     6661 tgccgttgac ggattgtatt ttgtcgccgc tttggaagcc ggcacgggcg gcaatggtgt
     6721 cgggttcgac tgtgccgacg taggggcgca gttcggttac gccgaaggaa aagctcagtc
     6781 cgtacagcaa aaccgccagt gcgaggttgg tcagtgggcc ggcggcgacg atggcgatgc
     6841 gcttggcggg gtgttgtttg tcaaaagcgt agggtaaatc ggcttctgat acttcgcctt
     6901 cgcgcgtgtc gaccattttg acgtaaccgc ccaacggaat cggggcgagg caccattcgg
     6961 tgtcgccgcg ctttcgggtg aaaaacggtt tgccgaagcc gacggaaaag cgtacgactt
     7021 tgacgccgca caatctggca acgatgtagt gtccgaactc gtgcaggctg accaaaatca
     7081 ggatggcgaa gataaaagct agaagggtgt gcaaatggtt ttcctttgat aacggtgttc
     7141 agacggcatc agcgcagtgt gccgataaat gctcgcgctt gtgcgcgtgt ccgggcatct
     7201 tgcgccaaca gcccttctat atcgcctatg ccgtctgaaa agtcttgtgc gagacaatgg
     7261 gcgacggttt tggcaatgtc ggtaaactta atctgtccgt ccaaaaaggc ggcgacggcg
     7321 gcttcgttgg cggcgttcaa tacgcagggc gcggctccgc ctgcgttcat ggcttcatag
     7381 gcgagcttca ggcaggggaa gcggtcaaag tcgggctttt ggaaggtcag cgcggacaat
     7441 gcgtcgaaat ccaggtcgcc gacacccgaa tcgatgcgct cgggcaaacc caaacaataa
     7501 gcgatgggcg ttcgcatatc gggattgccc agttgcgcca gcacggagcc gtcgcggtag
     7561 cgcaccatgc tgtgtatcac ggattgcgga tggatgacga cttcgagttt gtcgggcgga
     7621 cagttgaaca gccaatgcgc ttcaatcagc tccaaacctt tgttcatcat ggtggcggaa
     7681 tcgacggaga ttttgcgtcc catacgccaa ttggggtgtt tgaccgcttg gtcgggcgta
     7741 atgctgtcga acgtgtttaa atcggcggtc agaaacgggc cgccggaagc ggtcaggata
     7801 atcgaagcga tgccgtgttc gttcagacgg ccggcgtaat cgcgcggcaa aacttggaaa
     7861 acggcgttgt gttcgctgtc gacgggcagc actgccgcgc cgtttgcacg ggcggtttcc
     7921 ataaacaacg cgccggaaac caccagcgtt tctttgtttg ccagataaat ggttttgcct
     7981 ttttgcgccg ctgcgagcgc ggaaggcagc cccaccgccc cgacgatggc gcacatgaca
     8041 ccgctgactt cgtcggcaga ggcaacgtca accaatgcct gcgcgccgtg taaaacctga
     8101 gtcgccgtgc cgtcgcgttt caacagggct tcaagccggg cggcgtgttc cgcatcggca
     8161 acgacggcat attcggggtg gaacgtttga cattgagccg ccaatttctc gacctgctta
     8221 tgccctgcca gcgcgaatac gcggaatttt tcggggtggc gggagacaac gtccagcgtg
     8281 ctttcgccta tgctgccggt actgcctaat atggtcagga cttgtggtgt cataatgggg
     8341 ataactttat accggatgcc gtctgaagcg ttttcagacg gcatagaatc aatttaaaac
     8401 cgacatcatc gctgcataga cgctgataac ggcaatcagg ctgtcggtac ggtcgaacac
     8461 gccgccgtgt ccgggcagca gcttgctgct gtctttgatg cctgccgcgc gcttgagcca
     8521 gctttccaaa aggtcgccgc atacgctgac aacggtcagc accaaaccga ttaacacggt
     8581 atcgaaccag cctgtatcga atgccagcca gccggcactt cgtacggcgg tcatgtacac
     8641 tgccacgcaa accgcgccgc cgattgcacc ttcccagctt ttgccggggc tgattgccgg
     8701 cgcgattttg tgtttgccga acgccttgcc gctgaaatac gcgcaaatat cggcaaccca
     8761 caccaaaccc atcacggcga gcagcggcag ggcatcatcg ggatgcgggc gcagggatac
     8821 gagcgcgaac caaaacggca tgaccagaag ccagccgacg gcataaacct gccaaccgcc
     8881 gttgagcctc catttgaatc tcaaccataa aggcataacg gcgagccaaa atgccaaaac
     8941 aacataccaa accaaattag gcagcatcca gccgcccgca taggcaacca cgccgaaaac
     9001 caaggttgcg gcgaggtaat ggttggtttt aattttgcac aaaccgccca tacgggcata
     9061 ttcccacaag gcaatcaggg caatcagtcc gcaaaatgca gcccacaacc attgcggcgc
     9121 gtaaaacagc atgcccagca tcagcggcag cagccacatg gcggttatta cccgttgttt
     9181 cagcatattc agttcctttg ctgttcgata ggcagttgct cggaggtgcg tccgaaccgc
     9241 cgttcgcgtt tttggaacga agcgacggca tcgtccaaag ccttgccgtc aaaatcgggc
     9301 cacaaaatat cggtgaaata cagttctgca tatgccatct gccagagcag gaaattgctg
     9361 atgcgcgttt cgccgccggt gcggatgaac aaatccggtt ccggtgcatc gcccagcatc
     9421 aagtgtttcg ccagcgtgtc ttccgtaatc tcggatacgc cttcggcaat cagtttgttt
     9481 gccgcctgca aaatatccca gcggccgccg taatcggcgg caatgctcag ggtcaggccg
     9541 gtattgtttg ccgtcaacgc ttccgcctct tcgatgcctt gcagaatctg ccggttgaag
     9601 cgttcgcggc tgcccaatat cttcaggcgc atattgtttt cgtgcaggcg gcgtacctgt
     9661 ttttgcaaag cctgtaaaaa cagccccatc aggaacgaaa cttcgtcttc ggggcggcgc
     9721 cagttttcgg ttgaaaaggc aaacacggtc agatattgca cacccagttt ggcgcaatgc
     9781 ttcaccatat tttccaatgc gtccaaaccg cgtttgtgtc ccattatgcg cgggaggaaa
     9841 cgttttttcg cccaacggcc gttgccgtcc ataatcacgg cgatatgctt gggaatggcg
     9901 gtgtgttcca aaacggcctg cgtgctgctt ttcatgtctg cctttcgcgg ttcggcattc
     9961 aaatgccgtc tgaacgccga accgtgcagg ttaaattgcc atcaaatctt cttctttggc
    10021 agtcaggagt ttgtcggctt cggtaatgta tttgtcggtc agtttttgaa ccgcttcttc
    10081 gccgcgacgt gcctcgtctt cggaaatttc tttgtctttg aggagttttt tgatgtggtc
    10141 gttggcatcg cggcgcacgt tgcggataga gacgcggcct tcttccgctt cgccgcgtac
    10201 gactttaatc aggtctttgc ggcgttcctc ggtcagcatg ggcatcggca cgcggatcag
    10261 gtcgccgaca gctgccgggt tcagtcccaa gtttgaatcg cggatggctt tctcgacttt
    10321 ggccgccata ttgccctcaa acggtttcac gccgatggtg cgcgcgtcca gaagcgttac
    10381 gttggcaact tggctgacgg ggaccatgct gccccagtat tcgacttcca cttggtcgag
    10441 caggccggta tgcgcgcggc cggtacgcac tttcgccaga ttttctttca gtacttcgac
    10501 cgaacgctgc atcttgcctt cggctgtttt ttgaatatcg ttgatcatat tgttctttcg
    10561 gtgggataag gtgggcggga gaccgtctga acgcgtttca agccgttcag acggcataaa
    10621 gaccgttaac cgcgaatagt accgttattc gggcataacg acaaggtagg cggattgggg
    10681 atgccgtctg aagcgacagg cgtttcagac ggcatcgtgt ccgaccgtca gccgtgttcc
    10741 cgtgtttcaa gcaggctttg gcgcaggtgt tggcgttcgt gggcatccag ccatttgcgg
    10801 cgggtgcgtt gcagcaggat gacgagggcg gaaatttcct gacgcatatt ggtgctgagc
    10861 cagaggaagc cctgccattg gtagtggagg tgttcggcga gggcttccag ttcggggttg
    10921 atggcggtgt cgatgcggat gcggcgggcg tgtctgccgt tgataagggc gacggtttgt
    10981 tgcaggtcgg tttggagcag tgtgaagtgg cggtcgagca gccggatttc gctgccgttg
    11041 agtttgggag attgcagctt ggcggcagtg gtcaggagca gctcggtggt gttgacaatt
    11101 ttacggtggg cgtgctgcat ggcttccatc atggcggggc tgatgcggct ttcgcccgat
    11161 gtggcggcga ggtggctgcg gcttttgacc atgcgtgcgt tgatttggcg cattttaacc
    11221 atattctgct ccaaacgttc gcgcgtcata cgcctgccgt tgctgatttc ggcaatcatt
    11281 ttgctgcagt cggtcaggtt gtcggcaagc atgaaacgcc acatcagtgt ggatttcagc
    11341 ggcagcagtt tggcggcggc gatggcgatg gccgcgccga tgaggacgtt catcgcgcgc
    11401 atcaggccgc tgtcgaacca ttcgctgccg ttgtcgccga tgagcatgca catcgtcagc
    11461 cccgccagca tagggacgta gccgtttttg ccgaccgccg cccagccggc cagtgcgctt
    11521 gccgtgccga cggtgaggta gaagaggagg ttgccgtgga aataatgctg gttcagccat
    11581 aaaacgccca aacccgcgcc cagcccgatg accgtgccga gcatacgttc caccgccttg
    11641 gagtaaatcg ccccttgaaa ctggagcatg ccgaggacga cgaagacggt catccctatc
    11701 cactcgccgt gttggaggtg gagcagccgg gcggtggcgg tggcgaacag gacggctccg
    11761 ccgagccgca cggcgtggat gaggcggcgg tagcggtaac gttcgtagga gttgagccag
    11821 cggctgacga ggcggttgcg ttgcgaggtg ttcatatcgg ttgtgccgtc tgaagcggaa
    11881 atgtgaaaaa gcacaggctt cccgagggag ggtctgtgct tggtattggt gccggagaag
    11941 ggaatcgaac ccccgacctt cgcgttacga atgcgctgct ctaccgactg agctacaccg
    12001 gcgttttttc gtcatgatat atatgaacgg ttgtttgtgc aacttttcgg gcgggcggca
    12061 aggcagtgcg c
//
LOCUS       DAXIEB010000062        11930 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000062 DAXIEB010000000
VERSION     DAXIEB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.062"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(425..1468)
                     /gene="argC"
                     /locus_tag="ACFOA3_001339"
     CDS             complement(425..1468)
                     /gene="argC"
                     /locus_tag="ACFOA3_001339"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6898340.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEASDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1465..1746)
                     /locus_tag="ACFOA3_001340"
     CDS             complement(1465..1746)
                     /locus_tag="ACFOA3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898341.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            2108..3256
                     /locus_tag="ACFOA3_001341"
                     /pseudo
     CDS             2108..3256
                     /locus_tag="ACFOA3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            3265..3492
                     /locus_tag="ACFOA3_001342"
     CDS             3265..3492
                     /locus_tag="ACFOA3_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898342.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            3485..3838
                     /locus_tag="ACFOA3_001343"
     CDS             3485..3838
                     /locus_tag="ACFOA3_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898343.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            4238..4677
                     /locus_tag="ACFOA3_001344"
                     /pseudo
     CDS             4238..4677
                     /locus_tag="ACFOA3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5006..5308
                     /locus_tag="ACFOA3_001345"
     CDS             5006..5308
                     /locus_tag="ACFOA3_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898344.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5592..5744
                     /locus_tag="ACFOA3_001346"
                     /pseudo
     CDS             5592..5744
                     /locus_tag="ACFOA3_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            5892..7634
                     /gene="hrpB"
                     /locus_tag="ACFOA3_001347"
     CDS             5892..7634
                     /gene="hrpB"
                     /locus_tag="ACFOA3_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6898345.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7743..>11930
                     /gene="hrpA"
                     /locus_tag="ACFOA3_001348"
     CDS             7743..>11930
                     /gene="hrpA"
                     /locus_tag="ACFOA3_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC6898346.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIESGKPLSLETSTVASNIRLNNGNIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGLLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKN"
BASE COUNT         3406 a         2781 c         2860 g         2883 t
ORIGIN      
        1 aggcaacgcc gtactggttt ttgttaatcc actatattat ttttgacaca ttttcatgat
       61 tttaaatccc gttattccca agtgggcgga aaccggcaat gagaagccgc agagacttat
      121 cgggaaaaac ggcaagccct ccgccgtcat tcccgcgcag gcgggaatct agaaatgaaa
      181 aaccacggtg ttatcggaaa tgattgaaac cggagaaatg acggaatcga agcgtgtgga
      241 aatgtgtcgg aaattgctga agccggataa actagattcc cgcttttgcg ggaatgacgg
      301 gattttaggt ttttgttttc gtgggaatgg atgggcccaa agccgtccga accaaaaaaa
      361 accgccgctt tcagacggca tcaaaaaacc gcaggcacga tgcctgcggt ttgccgtttg
      421 cgcttcaggg gagcaggggg attgcgccca agccgtgtgt ttccttcagt ccgaacatga
      481 tgttcatatt ttgcaccgcc tgacccgccg cgcctttgac gaggttgtcg atgacggaaa
      541 ggacgaccca cacatcggat tgcgccgcct gttggatgct gatgcggcag aggtttgcgc
      601 cgcgcacgct gcgggtttcg ggtgtcgaac cggcgggcag gatgtccacg aacaggctgt
      661 cgcgataata gtcgcgcaaa acggtttcgg ggcagatgcc gtctgaaagg tggaggtaaa
      721 cggtggcgtg cataccgcgt atcatcggcg cgaggtgcgg cgtgaacacg aatccttcgg
      781 cgatgccgtc ctgaagcccg gcgatggtct gcctgatttc gggcaggtgg cggtgtccgg
      841 ctatgccgta ggctttgaag ttgtcgctgg cttcgcacaa cagcgaaccg acattgcctt
      901 tcctgcccgc gccggatacg ccggatttgc agtcggcaat cagcggcata ccgggcttca
      961 gacggcattg ccgcaacagc ggcacgagcg gtagggatac gcaggtcggg tagcagccgg
     1021 ggttggcgac gaggcgcgcc tgtgcgacgg cttcgcggtt gagttcgctc aatccgtaca
     1081 cggcttggga aacgaggccg ggggcggcgt gggtcatgcc gtaccagtgt tcccaggtcg
     1141 gaatgtcccg tatgcggaag tcggcggaaa ggtcgatgac gcgcacgccc tgttccaaaa
     1201 ggcgcagcgc gtctttcata gcgatgccgt tgggcgtggc gaagaagacg atgtcgcatt
     1261 gttccaaacc tgcctcgtcg ggcgtttgga aggcgaggcc gtacacgccg cgcagacttg
     1321 gaaagtaatc ggcaactgcg gttcccgctt cgctgcggct ggttacggcg gcgacttcga
     1381 catcgggatg ggcggcaagc aggcgcagca gttccacgcc ggtgtagccc gtcgcgccga
     1441 caatgccgac tttgattttt ttactcatgg tttttccttt gtgtggttgg cgggtatgcc
     1501 gtccgaacgc tgtccgacgg ggttttgatt ggcggcggaa ggacggtaca caaagcgttt
     1561 gaaaagcgtg tctaatttaa tggcatccgc ctctgttttt aaaacccagc cctgccgtcc
     1621 ggaataaaca taaccgtagg cgcgaaagtc ggcaggatat ttcggtgcct gccccaaacc
     1681 caaaccgtat gcggcgaaag cggtggtcgt ggagaaggaa agcaggagga ggacgagggt
     1741 tttcataagg ttccaaaccg aacggggcga tgaagcccga ttatagcaaa aggtcgtctg
     1801 aaaaccgttt tcagacgacc ttttatttaa ttacaataaa aatgttaaca acaaaaaaca
     1861 aaccgctttt tttcgtttgg aaacaaaaca aatttttata gtggattaaa tttaaaccag
     1921 tacagcgttg cctcgccttg ccgtactatt tgtactgtct gcggtttcgt cgccttttcc
     1981 tgatttttgt taatccacta taacaaaatt ggcaacaatt ccttgacgca cacacaaaaa
     2041 aaactttaat atttgtaaat atatgtaaca ttataaattt taaatatttt ctgagtaagg
     2101 ggaagtaatg agcatccatc tcgacttcgg cattagtcct aaaacgttcc gacagactta
     2161 tctgtatcaa aagcccaagc tctttaaagg agcggttcgg aatctcgaag ccgcatcttg
     2221 gaaagatatc aacgatatat accaacgagc agacccaacc gcaccgctgt ttcatctgcg
     2281 taaaaaaggt gcaattgttc ctaaagaaga atacgtcgaa agtttcgacg atttgggcaa
     2341 aactcgctac cgttttatta aatccgttat ctacgaacat atgaagaatg gcgcgtcgtt
     2401 gctctataac catattatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc
     2461 cggcgcacat accattgtta gtgggtatct tgcttttggc agcgacgaat cttataaaaa
     2521 ccattgggac atccgagatg tgtatgccat ccagcttttc ggcaagaaac gttggcaact
     2581 tactgcccct gatttcccta tgccattgta tatgcaacag actaaagata ctgatatttc
     2641 cattcctgaa catatcgata tggatattat ccttgaagca ggtgatgtcc tctacatccc
     2701 gcgcggttgg tggcacagac ctatcccgct cggctgtgaa accttccacc tcgctgtcgg
     2761 tacctttccg cccaacggct ataattacct cgagtggcta atgaagaaat tccccacgat
     2821 agaaagtctg cgccacagtt tctcagactg ggagcaagat aggacgcgta tcaacgatac
     2881 tgccgcacaa attgctgcca tgattgccga ccccgtcaat tatgaagcct tcagtgaaga
     2941 ctttctcggc aaagaacgta ccgataccgc ttttcatctc gaacagttcg cgaatcccaa
     3001 cgctactccg ctttcagacg acgtcaggtt gagattaaat gccaataatt tggatacgtt
     3061 ggaaaaggga tatttgattg ggaatgggat gaagataagc gtagatgagt tggggaaaaa
     3121 agttttagaa cacatcggta agaatgaacc gttattgttg aaaaatctac tggttaactt
     3181 caatcaggga aaatatgaag aagttaggaa gttgatttat cagttgatag agttagattt
     3241 tctggaaatt ttgtgaggga ttctatgaaa aactggaagc aatttacatt tttcgtaata
     3301 ttagtaatag cttgttatca agtgctatat tttttatcag atatgtttct tctcgactac
     3361 attaacaaat atagttggaa tttgaatttt attcagggta ctttgaattt tttttcaata
     3421 tatctgccat atgtttttgt tagccgaatt tttaggaata ccaaccaaga aaaggagtat
     3481 aaaaatgatt gaacttcaac ttcatgaatt gaagctggtt tcagggggag gtcctgtaac
     3541 agacaatata gctggaaatg tagctaatgc tgccacaacc aaaggaggtc ccacatgggg
     3601 ggatttggtt gcaatacctg ctgccgcagc ggcagtttat tatctgccga aaaatgctta
     3661 cggtgctgct ggtgcaaatg gtgtatacaa tgtgactcgt gattgggtaa atgatgccgt
     3721 taatgcacct ccttataacg gaagaccaat ctttgagatt gaacatggat taactgctcc
     3781 cgcaacaaaa gcagataaat caggaaacgg ctacactgac ggtacagatt actgctgata
     3841 tttctcattg ttcagacgat ctctaggggt cgtctgaaac tttcttaact tcaattttat
     3901 gaatagaccc aagcaaccct tcttccgtcc cgaagtcgcc gttgcccgcc aaaccagcct
     3961 gacgggtaaa gtgattctga cacgaccgtt gtcattttcc ctatggacga catttgcatc
     4021 gatatctgcg ttattgatta tcctgttttt gatatttggt aactatacgc gaaagacaac
     4081 agcggaggga caaattttac ctgcatcagg cgtaatcagg gtgtatgcac cggatacggg
     4141 gacaattaca gcgaaattcg tggaagatgg agaaaaggtt aaggctggcg acaagctatt
     4201 tgcgctttcg acctcacgtt tcggcgcagg aggtagcgtg cagcagcagt tgaaaacgga
     4261 ggcagttttg aagaaaacgt tggcagaaca ggaactgggt cgtctgaagc tgatacacgg
     4321 gaatgaaacg cgcagcctta aagcaactgt cgaacgtttg gaaaaccaga aactccatat
     4381 ttcgcaacag atagacggtc agaaaaggcg cattagactt gcggaagatc agtagcaaaa
     4441 acggcactgg gcagacagga attgtcggga ttgggcatgg tatcctccga tttggcgaag
     4501 agcaacgaac ctgtttatct tgtgaaaata aaacccgaca aaccaaccat cactgcatac
     4561 ggtgaggaaa aaccgctgca aatcggcatg acgttggaag cagacatcct gcacgagaaa
     4621 cggcgattgt atgaatgggt attggagccg atttatagta tatcgggcaa actgtaagac
     4681 ttgaggctga aagataaaga agttgtctga aaccttttca gacgaccaga agacaatata
     4741 taaaaacaca aacttcaaaa ctgcctgaaa ccatcccctt ttacaggcac tccgtctaca
     4801 ggcactccgt ctgtaaaagc aaggataggg catctgccct tttgattgtt tgattaacga
     4861 tacaaggagt ttcaaaatga gagttttata gtggattaaa tttaaaccag tacagcgttg
     4921 cctcgccttg ccgtaccatt tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat
     4981 ttaatccact atatgtgttc atgaaatgac ttgggtcgga ggctcaggta atgcgcaaca
     5041 aagttcatat tattgcgaaa tttgcgaatc tgcagggctt aacgatacgg gaaatcctga
     5101 taaatcttta ggattgccaa acaatacgtt cagtaatccg cctggttggg gagctacaat
     5161 cggagcttta gcaggtagcc gcatagggat gcctgaattt ggtacgtttg cgagccatgc
     5221 cattgaaaat ttcgactggt catggtatcg acgttatagg gaaattgccg aaacgattga
     5281 acgagaatat tcaggcggtt tgccttaata gttgaggagg tcatgatgtt tgccaaacat
     5341 tatcaattca tcgcactcgg catcatgctg cttctttata tgttgattct ctatacgacc
     5401 gatttttcca atctgacgta ttggatgctg ttttttatct gttttattac aggaaaaata
     5461 ttagctcgtt tgttagagaa aagctttaaa taaaatagca gctagtcgca aaaggtcgtc
     5521 tgaaaccttt tcaggcggcc tttctaaaat acatccaact tcctaatccc tatttttcaa
     5581 aaaggaaatc tatgccccat ctgcaaaacc tgtctttggg cttaaagaaa aagctgcctg
     5641 ttatcctgca aacagaaata tcagaatgcg gcttggcatg tctggcggct gtggcgggat
     5701 ttcatggttt ccatacgaat ttacgcgcac tgcgttcaaa atactgtccg agacctttgc
     5761 aaaattcccc aaaatcccct aaatgtcttg gtgggaattt tggggaattt tgcaaaggtc
     5821 tcattctata actgtaaata cttttaaatt tatgacaaaa tagtaaatat tgctaaaata
     5881 atattgatgt catgaaattt tttcctgctc catgtctgtt ggttatcctg gctgtcatac
     5941 cccttaaaac cttagctgcc gatgaaaacg atgcagaact tatccgttcc atgcagcgtc
     6001 agcagcacat agatgctgaa ttgttaactg atgcaaatgt ccgtttcgag caaccattgg
     6061 agaagaacaa ttatgtcctg agtgaagatg aaacaccgtg tactcgggta aattacatta
     6121 gtttagatga taagacggcg cgcaaatttt cttttcttcc ttctgtgctc atgaaagaaa
     6181 cagcttttaa aactgggatg tgtttaggtt ccaataattt gagcaggcta caaaaagccg
     6241 cgcaacagat actgattgtg cgtggctacc tcacttccca agctattatc caaccacaga
     6301 atatggattc gggaattctg aaattacggg tatcagcagg cgaaataggg gatatccgct
     6361 atgaagaaaa acgggatggg aagtctgccg agggcagtat tagtgcattc aataacaaat
     6421 ttcccttata taggaacaaa attctcaatc ttcgcgatgt agagcagggc ttggaaaacc
     6481 tgcgtcgttt gccgagtgtt aaaacagata ttcagattat accgtccgaa gaagaaggca
     6541 aaagcgattt acagatcaaa tggcagcaga ataaacccat acggttcagt atcggtatag
     6601 atgatgcggg cggcaaaacg accggcaaat atcaaggaaa tgtcgcttta tcgttcgata
     6661 accctttggg cttaagcgat ttgttttatg tttcatatgg acgcggtttg gtgcacaaaa
     6721 cggacttgac tgatgccacc ggtacggaaa ctgaaagcgg atccagaagt tacagcgtgc
     6781 attattcggt gcccgtaaaa aaatggctgt tttcttttaa tcacaatgga catcgttacc
     6841 acgaagcaac cgaaggctat tccgtcaatt acgattacaa cggcaaacaa tatcagagca
     6901 gcctggccgc cgagcgcatg ctttggcgta acaggtttca taaaacttca gtcggaatga
     6961 aattatggac acgccaaacc tataaataca tcgacgatgc cgaaatcgaa gtgcaacgcc
     7021 gccgctctgc aggctgggaa gccgaattgc gccaccgtgc ttacctcaac cgttggcagc
     7081 ttgacggcaa gttgtcttac aaacgcggga ccggcatgcg ccaaagtatg cccgcacctg
     7141 aagaaaacgg cggcggtact attccaggca catcccgtat gaaaatcata accgccggat
     7201 tggatgcagc ggccccgttt atgttgggca aacagcagtt tttctacgca accgccattc
     7261 aagctcaatg gaacaaaacg cctttggttg cccaagacaa gttgtctatc ggcagccgct
     7321 acaccgttcg cggatttgat ggggagcaga gtcttttcgg agagcgaggt ttctactggc
     7381 agaatacttt aacttggtat tttcatccga accatcagtt ctatctcggt gcggactatg
     7441 gccgcgtatc tggcgaaagt gcacaatatg tatcgggcaa gcagctgatg ggtgcagtgg
     7501 tcggcttcag aggagggcat aaagtaggcg gtatgtttgc ttatgatctg tttgccggca
     7561 agccgcttca taaacccaaa ggctttcaga cgaccaacac cgtttacggc ttcaacttga
     7621 attacagttt ctaacctctg aattttttac tgatatttag acggtctttc cttatcctca
     7681 gactgtcaaa ctttacctac gtacttggcg cgcagtacgt tcatcttcaa aatggaatag
     7741 acatgaataa aggtttacat cgcattatct ttagtaaaaa gcacagcacc atggttgcag
     7801 tagccgaaac tgccaacagc cagggcaaag gtaaacaggc aggcagttcg gtttctgttt
     7861 cactgaaaac ttcaggcgac ctttgcggca aactcaaaac cacccttaaa accttggtct
     7921 gctctttggt ttccctgagt atggtattgc ctgcccatgc ccaaatcacc accgacaaat
     7981 cagcacctaa aaaccagcag gtcgttatcc ttaaaaccaa cactggtgcc cccttggtga
     8041 atatccaaac tccgaatgga cgcggattga gccacaaccg ctatacccag tttgatgtcg
     8101 acgctaaagg tgctgtactg aacaacgatc gcaacaacaa cccgtttctg gccaaaggca
     8161 gtgcgcagtt gattttgaac gaggtgcgcg gtgcggccag caaactcaat ggcatcgtta
     8221 ccgtaggtgg tcaaaaggcc gatgtgatta ttgccaaccc caacggcatt accgttaatg
     8281 gcggcggctt taaaaatgtc ggtcggggca tcttaactac cggtgcgccc caaatcggca
     8341 aagacggtgc actgacagga tttgatgtgc gtcaaggcac attgaccgta ggagcagcag
     8401 gttggaatga taaaggcgga gccgactaca ccgagatact tgctcgtgca gttgctttgc
     8461 aggggaaatt acagggtaaa aacctggcgg tttctaccgg tcctcagaaa gtagattacg
     8521 ccagcggcga aatcagtgca ggtacggcag cgggtacgaa accgactatt gcccttgata
     8581 ctgccgcact gggcggtatg tacgcagaca gcatcacact gattgccaat gaaaaaggcg
     8641 taggcgtcaa aaatgccggc acactcgaag cggccaagca attgattgtg acttcgtcag
     8701 gccgcattga aaacagcggc cgcatcgcca ccactgccga cggcaccgaa gcttcaccga
     8761 cttatctctc catcgaaacc accgaaaaag gagcggcagg cacatttatc tccaatggtg
     8821 gtcggatcga gagcaaaggc ttattggtta ttgagacggg agaagatatc agcttgcgta
     8881 acggagccgt ggtgcagaat aacggcagtc gcccagctac cacggtatta aatgctggtc
     8941 ataatttggt gattgagagt aaaactaatg tgaacaatgc caaaggctcg gctaatctgt
     9001 cggccggcgg tcgtactacg atcaatgatg ctactattca agcgggcagt tccgtgtaca
     9061 gctccaccaa aggcgatact gaattgggtg aaaatacccg tattattgct gaaaacgtaa
     9121 ccgtattatc taacggtagt attggcagtg ctgctgtaat tgaggctaaa gacactgcac
     9181 acattgaatc gggcaaaccg ctttctttag aaacctcgac cgttgcctcc aacatccgtt
     9241 tgaacaacgg taacattaaa ggcggaaagc agcttgcttt actggcagac gataacatta
     9301 ctgccaaaac taccaatctg aatactcccg gcaatctgta tgttcataca ggtaaagatc
     9361 tgaatttgaa tgttgataaa gatttgtctg ccgccagcat ccatttgaaa tcggataacg
     9421 ctgcccatat taccggcacc agtaaaaccc tcactgcctc aaaagacatg ggtgtggagg
     9481 caggcttgct gaatgttacc aataccaatc tgcgtaccaa ctcgggtaat ctgcacattc
     9541 aggcagccaa aggcaatatt cagcttcgca ataccaagct gaacgcagcc aaggctctcg
     9601 aaaccaccgc attgcagggc aatatcgttt cagacggcct tcatgctgtt tctgcagacg
     9661 gtcatgtatc cttattggcc aacggtaatg ccgactttac cggtcacaat accctgacag
     9721 ccaaggccga tgtcaatgca ggatcggttg gtaaaggccg tctgaaagca gacaatacca
     9781 atatcacttc atcttcagga gatattacgt tggttgccgg caacggtatt cagcttggtg
     9841 acggaaaaca acgcaattca atcaacggaa aacacatcag catcaaaaac aacggtggta
     9901 atgccgactt aaaaaacctt aacgtccatg ccaaaagcgg ggcattgaac attcattccg
     9961 accgggcatt gagcatagaa aataccaagc tggagtctac ccataatacg catcttaatg
    10021 cacaacacga gcgggtaacg ctcaaccaag tagatgccta cgcacaccgt catctaagca
    10081 ttaccggcag ccagatttgg caaaacgaca aactgccttc tgccaacaag ctggtggcta
    10141 acggtgtatt ggcactcaat gcgcgctatt cccaaattgc cgacaacacc acgctgagag
    10201 cgggtgcaat caaccttact gccggtaccg ccctagtcaa gcgcggcaac atcaattgga
    10261 gtaccgtttc gaccaagact ttggaagata atgccgaatt aaaaccattg gccggacggc
    10321 tgaatattga agcaggtagc ggcacattaa ccatcgaacc tgccaaccgc atcagtgcgc
    10381 ataccgacct gagcatcaaa acaggcggaa aattgctgtt gtctgcaaaa ggaggaaatg
    10441 caggtgcgcc tagtgctcaa gtttcctcat tggaagcaaa aggcaatatc cgtctggtta
    10501 caggagaaac agatttaaga ggttctaaaa ttacagccgg taaaaacttg gttgtcgcca
    10561 ccaccaaagg caagttgaat atcgaagccg taaacaactc attcagcaat tattttccta
    10621 cacaaaaagc ggctgaactc aaccaaaaat ccaaagaatt ggaacagcag attgcgcagt
    10681 tgaaaaaaag ctcgcctaaa agcaagctga ttccaaccct gcaagaagaa cgcgaccgtc
    10741 tcgctttcta tattcaagcc atcaacaagg aagttaaagg taaaaaaccc aaaggcaaag
    10801 aatacctgca agccaagctt tctgcacaaa atattgactt gatttccgca caaggcatcg
    10861 aaatcagcgg ttccgatatt accgcttcca aaaaactgaa ccttcacgcc gcaggcgtat
    10921 tgccaaaggc agcagattca gaggcggctg ctattctgat tgacggcata accgaccaat
    10981 atgaaattgg caagcccacc tacaagagtc actacgacaa agctgctctg aacaagcctt
    11041 cacgtttgac cggacgtacg ggggtaagta ttcatgcagc tgcggcactc gatgatgcac
    11101 gtattattat cggtgcatcc gaaatcaaag ctccctcagg cagcatagac atcaaagccc
    11161 atagtgatat tgtactggag gctggacaaa acgatgccta taccttctta aaaaccaaag
    11221 gtaaaagcgg caaaatcatc agaaaaacca agtttaccag cacccgcgac cacctgatta
    11281 tgccagcccc cgtcgagctg accgccaacg gtatcacgct tcaggcaggc ggcaacatcg
    11341 aagctaatac cacccgcttc aatgcccctg caggtaaagt taccctggtt gcgggtgaag
    11401 agctgcaact gctggcagaa gaaggcatcc acaagcacga gttggatgtc caaaaaagcc
    11461 gccgctttat cggcatcaag gtaggtaaga gcaattacag taaaaacgaa ctgaacgaaa
    11521 ccaaattgcc tgtccgcgtc gtcgcccaaa ctgcagccac ccgttcaggc tgggataccg
    11581 tgctcgaagg taccgaattc aaaaccacgc tggccggtgc cgacattcag gcaggtgtag
    11641 gcgaaaaagc ccgtgtcgat gcgaaaatta tcctcaaagg cattgtgaac cgtatccagt
    11701 cggaagaaaa attagaaacc aactcaaccg tatggcagaa acaggccgga cgcggcagca
    11761 ctatcgaaac gctaaaactg cccagcttcg aaagccctac tccgcccaaa ttgtccgcac
    11821 ccggcggcta tatcgtcgac attccgaaag gcaatctgaa aaccgaaatc gaaaagctgt
    11881 ccaaacagcc cgagtatgcc tatctgaaac agctccaagt agcgaaaaac
//
LOCUS       DAXIEB010000063        11762 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000063 DAXIEB010000000
VERSION     DAXIEB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.063"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(119..1411)
                     /locus_tag="ACFOA3_001349"
     CDS             complement(119..1411)
                     /locus_tag="ACFOA3_001349"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6898347.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1489..2457
                     /locus_tag="ACFOA3_001350"
     CDS             1489..2457
                     /locus_tag="ACFOA3_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898348.1"
                     /translation="MKLTLAIPSLNLDKDETRIPLCLHAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2658..4124)
                     /locus_tag="ACFOA3_001351"
     CDS             complement(2658..4124)
                     /locus_tag="ACFOA3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6898349.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYQNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4366..6477)
                     /locus_tag="ACFOA3_001352"
     CDS             complement(4366..6477)
                     /locus_tag="ACFOA3_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6898350.1"
                     /translation="MTLKASKQASKQASKQASKQASKQASGRVNLLTLSVLSLFCTPY
                     AWGADEYDPEVLPRFILEAAENRDSHVWRGPCSISFDCFGMFRAQLGSNTRSTKIGDD
                     ADFSFSDKPKPGTSHYFSSGKTDQNSSEYGYDEINIQGKNYNSGILAVDNMPVVKEYI
                     TKNYGTDLKQAVKSQLQDLYKTRPEAWAENKKRTEEAYIAQFGTKFSTLKQTMPDLIN
                     KLVEDSVLTPHSNTSQTSLNNIFNKTLHVKIENKSHVAGQVLELTKMTLKDSLWEPRR
                     HSDIHTLETSDNARIRLNTKDEKLTVHKAYKGGADFLFGYDVRESDEPALTFEEKVSG
                     KSGVVLERRPENLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCEGGFCLGV
                     QRLAIPEAEAVLYAQQAYAANTLFGLRAADRGGDVYAADPSRQKLWLRFIGGRSHQNI
                     RGGAAADGWRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHGY
                     GGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYNAL
                     VAEGVVGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTR
                     FALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSAR
                     IGYGKRTDGDKEAALSLKWLF"
     gene            complement(7409..7654)
                     /locus_tag="ACFOA3_001353"
     CDS             complement(7409..7654)
                     /locus_tag="ACFOA3_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898351.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIASIIH
                     GGLPVLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            7766..8194
                     /locus_tag="ACFOA3_001354"
     CDS             7766..8194
                     /locus_tag="ACFOA3_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6898352.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            8206..8682
                     /locus_tag="ACFOA3_001355"
     CDS             8206..8682
                     /locus_tag="ACFOA3_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6898353.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(9032..9520)
                     /locus_tag="ACFOA3_001356"
     CDS             complement(9032..9520)
                     /locus_tag="ACFOA3_001356"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6898354.1"
                     /translation="MLKITLIAACAENLCIGADNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLHVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYDQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(9588..10643)
                     /gene="aroG"
                     /locus_tag="ACFOA3_001357"
     CDS             complement(9588..10643)
                     /gene="aroG"
                     /locus_tag="ACFOA3_001357"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6898355.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            complement(10805..11032)
                     /locus_tag="ACFOA3_001358"
     CDS             complement(10805..11032)
                     /locus_tag="ACFOA3_001358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898356.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            11310..11567
                     /locus_tag="ACFOA3_001359"
     CDS             11310..11567
                     /locus_tag="ACFOA3_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898357.1"
                     /translation="MTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(11549..>11762)
                     /locus_tag="ACFOA3_001360"
     CDS             complement(11549..>11762)
                     /locus_tag="ACFOA3_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898358.1"
                     /translation="YILDNARFHRMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWAN
                     IKRYLQTVLSDYARFDDALLSYFDFN"
BASE COUNT         2668 a         3276 c         2883 g         2935 t
ORIGIN      
        1 gtacaggaat gacagcagtg cttccttggc ttaattttat tttaatcaac tgcaaatgcc
       61 gtctgaaaac cggtttgcag attttcagac ggcattgttt tcgtcgccgg ttcaaacttc
      121 aatccgattg cgacacggta accggtatgc cgcgcaagcg cgcaagcagc gcgtccgcct
      181 tctgtttctg cgcggcaaac gccgccttgt ccgcctgcgt caattcgggt gtcggcaatg
      241 cgactgaaac aggattgacg ggctgcccgt tgatgcgtgc ctcgtaatgc aggtgcggcc
      301 cggtcgaacg cccggtcgaa ccgacaaaac cgatgacctc gccgccgcgc acattgcctt
      361 gtgcctgcga aaacgcgctc aagtgcgcgt acagcgtttc cacgccgttg gcgtggcgta
      421 tcatcaccgc gttgccgtat ccgcccttcc ggcctttaaa ggtaatcacg ccgtcggcgg
      481 aagccctgac cggcgttccc tgcggtgcgg cataatcgat gcccgtgtgc agccgccaag
      541 tgtgcaggat ggggtgcatg cggtagccga acggcgaaga aatgcgcgta tagaccagcg
      601 gctcgatgtt gaagccgcct ttttcctgca acaccctgcc gtcttcatcg taataattgc
      661 cgccccctcc gccttccttg tccgaacggt aatagaacgc ctgatgcctt gtgccgccct
      721 taacgacttc agccgccaaa atatcgcccg ccgccacctg ctgcccgtgg aaatacaggc
      781 tgtcgtaaat caggcgcacg gcatcgcctt ccttcaaacc gtcaaggctg aagcggccgg
      841 cgaaaatccc gcttaaggat tcgcggattt cgacgggcac ttccgcccgc gccagcgaac
      901 cgcgcgccga cgttttgacc acgaccgaac gcagcgtcgg caaaaccttc atatccgcct
      961 cagaagccga ccgccgccat atgccgcctt tcttttccaa agcgaccaga ttgcgctcgc
     1021 cgtcttcgtc ggtaaaaaac tgcacttcgc gcgcgccgcc gtcgccgccg accaaaacat
     1081 gaaccgactg gtcagcacgc aaatgccgca aatcggcttc gccgccatat ttttccgtga
     1141 ttcgggcaat ctcgtcccgc gccatacccg aacgcgccag cacgtccgcc agcgagtcgc
     1201 ccggctgcac cgcctcctgc acccaatatg ccgtctgaac accgctgccg ccccaagaca
     1261 gcggcggcag attttgttcc acgcgctgcg gtctgacgcg ctccgtcctc tctgtcgaag
     1321 caatgtatgc cgctgacacc aaaataatcg aaacggcaag cgcgcgcagc gcgtatttcc
     1381 gatgttttgc cgaaagtggg aagacagcca tattgttcct ttttgacggc gtttgcgccg
     1441 aatcctctat aatgttgcgc ctgacctgca tttctgaaac gtcaaacgat gaaactcacc
     1501 ctcgccattc cctcgctcaa tttggataaa gacgaaaccc gaatacctct ctgcctgcac
     1561 gccttcaaca aaatcctgca atacggctcg ctgcaccgcc aaagctgcac ggcttcggca
     1621 ttttacgcgc gttatttgtg gtgcggcaga ctggcggaac gcgcggcgca aagcctgaat
     1681 atgccgtctg aaaccgtcgc gctcgccacg cccgtctggc aaaagatggg gatgcatcag
     1741 gcaaatgtgc tgacggcgga atatttggat gtcggtacgg atgaagcgga acggctctgc
     1801 cgcgatctgt cggcgtttta cggcgatatt ccgtggcgtt tcgttcccgt cctgcccgaa
     1861 ttatggctgg tttcccttcc ccgcgcgtac cgctggggcg cgaagcccgt tttggacttg
     1921 ggcggactgt tgggtgcgga cgatcaaccg gacggggaag acgcgttgga atggctgcgc
     1981 gtccaaaccg aaatccaaat gtggctggcg gcgcaccccg tcaaccataa ccgcaagaaa
     2041 cgcgggctgc ccgagttgaa cggcttatgg ctttgggaca gtctgcacgg aagcagtcaa
     2101 ggcggcacgc ttttcgccga tacggtttgg agccgtttcc accccgaccg ccgcgcattg
     2161 cccgacaact tccgcgccta tgaagaaacg gcggcacacc tgcccgacac gcaccatatc
     2221 ctgtttatgg acgatttgcg cctgaccgcc ctgacagggg acagggagcg gtatgcatcg
     2281 gttttacagc agtgggaaga acactggttc gcaccgatct acgaagccgt ccgtaccggc
     2341 aaaatcaaac ggctggacat caccacggac ggacaacacg gcggtacgct gaccttcaaa
     2401 cccgccgaca ggtggaaatt ttggcgcggc gcgaaaacct ttgacggaat ctggtgaacc
     2461 cgcccgccgc aaccgcgaca atgccgtctg aagcccgaat cgggtttcag acggcatttt
     2521 acctcgcatc gccgttatgc cggaaaaacc ggaatgcgtt tccgcacacg cggcggcacg
     2581 caaagcaccg cccgaaagcc gccgatgccc gccgtgatac cgccgtcggc agggtttcct
     2641 ccgaaaagga acagcaatca gaacgtttta ttaaactcga caaacgcccg atttttctcg
     2701 tattcgttga acacatcgtt actccacgtt tcgcggtgcg acaacgtcag gcgcggcgtg
     2761 atgcctttga aatgcaatgc ccggtgccaa aggctcaagg atgtgttcaa ttctttatcc
     2821 ctgcgccttt cccctttaaa accgctgaaa aagccgggtt tttcataatg ccgtttcgcc
     2881 gcgccgaggc gcaacagcga agacaggccg ctgccgcccc attcctgccc ccaggcaaag
     2941 cgcaggccgt aacggttgaa attgtcgccc cggtcggcgg ggttgcgctc gcggtaaaaa
     3001 tccaaaccgc ccatccaata ttggcgcgca ttctggtaaa acaccagcga attggaaatt
     3061 tgcaaatggg tattgtcgga acgcgcccgg cgcgtattct tcaaacgccc ccactccgcc
     3121 gaagacaacg tttgccattt cggggtttgc caacggttga aataaaggcg tgcgccgttg
     3181 gtgtaagaat aagcgtcgtt gccgtaggtg cggcgttcgt ggaacactgc cagcccggca
     3241 tctttgcgcc ggtcggcaaa accgatgccg ccggaaacgc ctgccgtcat atcgttgaat
     3301 ttcttattcc ccggataaac cctgccggac acgtcgccgc ccgccgtcgt gtaccagccg
     3361 tttttcagcg accatttttt ctccgcgccg agccggtaat tgaccgccgt gccgtccacc
     3421 tgtttcggga aagtccattt gccgtactgc tgccgtttcg gggcttggtt gatattgtgt
     3481 tcgcgggtga cgctgaagcc gccatttacc ttccacgcat cgcgttcgcg caatgccttg
     3541 cggtacagct cgacctgctc catcagctgc ggcggcaggt tttccgcctt caggcggtcg
     3601 aactggtctg ccgccgcctc gttctgcctg tcttcaaaca atgccgccgc caaacgcata
     3661 cggacggcgg gcgcgtcggg ttgggcggca atcaattccc ggtaatggga aatcgcttct
     3721 ttcaccctac cgtctgcctg cgccaaaatc ccttgtgcat aaagtgccaa catcttatcc
     3781 tgctgcgcct gttgtaggta aatcggcaaa ataacgcgga taccggcaat attgtttgag
     3841 accactgcgg aatacatcgc gcgggacagc aattcgggat ttttcagcag ggtttcgccg
     3901 tcaatctgca aaacttttcc tttttcccgc acctgccccg gtaccttctc cctgtcgatt
     3961 ggtttgacct ccgcttcatg aagcctgaac tcgggacggc ttctcaaatc cggttcgcgc
     4021 ggtgtttctt cggcatatgc cgcgcttgcc aataaaggca gcagcagcat ccatctgttt
     4081 cgtgcaggca taaatgtctt cccacaaaaa taaaaaataa ccattatcaa ccggcggatt
     4141 gtatcagttt tttatgccgc tgttccaaac cctgccgccc gatgccgcga caatgccgtc
     4201 tgaagccaga atcgggtttc aaacggcggc aaaaaaccgg cggttttccg ccggtttcat
     4261 cattatgccc cgcctttcgg ggcggcaggg ttcggattgg tttgcggggc ggcgcgcggt
     4321 gccgtgccgg tgtgccgcct gtacgtcaaa acatttcccg gcgcatcaaa acagccattt
     4381 gagcgacaat gcggcttctt tgtcgccgtc cgtccttttg ccgtatccga tgcgtgcgga
     4441 catatggcct ttccaaccgg cttcaatgcc gaaccgccct tcgagcgccg tcctgcctgc
     4501 cagcgtctgt ttttcgccgt ccatttccac gccgaaagat tttgacctgt gcaaaacatt
     4561 aaaagcggca aaaggctgaa gattgacacc gttacgcaaa gcaaaacggg tttttgcccg
     4621 aatgccggcg cggctttgcc actgaccgct gccgagcagt ccgaccgccg tcccctcgct
     4681 gtcggtaaag ccgccgttta cgcccaagta ggtaaactgc gcctgcggtt gcaggtaaaa
     4741 ccgcacatta ttgccttttc cgacaacgcc ttccgccaca agcgcgttgt agccgccttc
     4801 gacagaagcc gtccaacctt tggttttgta gcgttccgca cggttttcat cattgatgcg
     4861 gtgtttgaaa cgttggtatt gcaaccagcc gtccaaatac gcacccgttt gtttatcgcg
     4921 caactgatgc cacgcagcat aaacaccccc gccataacca tgcaaataac tgcctgccgc
     4981 accgcctttg ccgttgactg atgcgtgctg gccagccctg ccgcccatca cgccgattgc
     5041 cagccggctg ccttcatttt gccgtacaaa cacctcgccg ccgatttgca cgcctttgcg
     5101 ccacccgtcc gcagccgcgc cgccccgtat attttgatgc gaccgaccgc cgatgaagcg
     5161 cagccacaat ttttgacggg acggatcggc ggcatacacg tcgccgcccc tgtcggcggc
     5221 acgcagccca aacaaagtat ttgccgcata agcctgttgg gcatataaaa ccgcttccgc
     5281 ctcagggata gccaaacgct gcacgcccaa gcaaaatccg ccttcgcatt gcttgagcaa
     5341 taattcgtac agtccctgcc ggtaattttg tttaaacgca aacgaattag agtctgcctt
     5401 ttccgccgca atcagtttgc gcccgtcgag cgttttcaga ttttccggcc ggcgttccaa
     5461 aaccacgccg gattttccgc tgactttttc ttcaaaggtc agggcgggtt cgtccgactc
     5521 ccgcacgtcg tagccgaaca ggaaatccgc accgccctta tacgctttat ggacggtcag
     5581 tttttcatct ttcgtgttca ggcggatgcg ggcattatcg gaagtttcca gcgtatggat
     5641 gtcggaatgg cggcgcggtt cccaaaggga atctttcagc gtcatcttgg tcagttccaa
     5701 cacctgtccg gcgacgtggg atttgttttc gattttgacg tgcagtgttt tattgaagat
     5761 gttgttgaga ctagtctgtg atgtattact atgaggagtg agtacggaat cttctaccaa
     5821 tttattaatt aaatcgggca tcgtctgttt gagcgtacta aattttgttc caaactgtgc
     5881 tatatacgcc tcctcagtcc gttttttatt ttctgcccaa gcttccggtc ttgttttgta
     5941 taaatcctgt aattgacttt taaccgcctg ctttaaatca gttccgtaat tctttgtaat
     6001 atattcctta acaacaggca tattatcgac ggcgaggatg ccgctattgt aatttttacc
     6061 ttggatattg atttcgtcat acccatattc ggatgaattt tgatcggttt taccgctgga
     6121 aaaataatgg gaagtgccgg gtttcggctt gtctgaaaat gaaaaatcgg catcgtcgcc
     6181 gattttggta gaacgagtat ttgaaccaag ctgagctctg aacataccga agcaatcaaa
     6241 agatatgctg cacggaccgc gccaaacgtg ggaatctctg ttttccgccg cttctaaaat
     6301 gaatcgcgga agaacttcag gatcatattc atccgcaccc caagcatatg gcgtgcaaaa
     6361 cagcgataaa acagataatg ttaatagatt aacccgaccg cttgcttgct tgcttgcttg
     6421 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgctttca gtgtcatgag
     6481 aaattttcct ttgtcaagtg ttaaaattat aatgattata tactacatcg cactacatcg
     6541 caatgaaaaa tcaggaaaaa ctaaaaaccc ctccgccgtc attcccgcga aagcgggaat
     6601 ctagaatttt aatgccgcaa gaatttatca aggatggtcg gggtttaaag gtctggattc
     6661 ccgcctgcgc gggaatgacg gtttagaaat cacccaaaac aagaaaaaga accgaaaccg
     6721 aacaagccgg attcccgcct gcgcgggaat gacgggattt caggttactg tttttgattt
     6781 tctgttttgt ggaaataacg ggattttgga tgatgtgcat ttatcgagaa aactaaaaac
     6841 cctccgccgt cattcccgcg aaagcgggaa tctagacctt tcggtgcgga aacttatcgg
     6901 ataaaacggt tttccctgca tcctggattc ccactttcgt gcgaatgacg ggatataggt
     6961 ttcactacgg acgtgttcag attaccgcct gcgcgggaag gacgggattt caggttgctg
     7021 tttttgattt tctgtttttg tgggaataac gggactttgg atgatgtgca tttatcggga
     7081 aaacaaaaaa ccctccgccg tcattcccgc gaaagcggga atctagaatt ttaatgctgc
     7141 aagaatttat cggggatgac cgaggtttga aggtctggat tcctgcctgc gcgggaatga
     7201 cggtttagaa atcacccgaa acgagaaaaa gaaccgaaac cgaacaagcc ggattcccac
     7261 ctgcgcggga atgacggctc aaaagttacg aaacgaaaaa taaccgaaac cagacaagtc
     7321 ggattcccgc gcgggcggga atgacgataa gcgtaatttc aatctgccat attgtctttt
     7381 cacttgcctg aaaacagatt tcagccgcct atccctgcat ccggcggctg atgtagtcgg
     7441 caatcaaatc ccgcttttcc caaaaaaatt tctgcatttt ttgttcggaa tacagtttgc
     7501 gaaccagtac gggcaggccg ccatgaataa ttgaggcaat ttctttgttg cctttttcct
     7561 cccgcatcgc cgtctgaaaa tccaaacccc tgcctttcag atatgctgca aggcggttgc
     7621 ggatgtggga attgaattcg atggatttga gcattttgcc tccgttttgc ggtaaggatt
     7681 tattcgattt gtttatccgc ctgattttat cacgtttccc aagcggtttg cctgtacaat
     7741 accgcttttc ggcagggtgt gggcgatgat gttttcttgg ttcaagctgt ttcacttgtt
     7801 ttttgtcatt tcgtggtttg cagggctgtt ttacctgccg aggattttcg tcaatatggc
     7861 gatgattgat gcgccgcgcg gcaatcccga gtatgtgcgc ctgtcggata tggcggtgcg
     7921 gctgtaccgt tttatgtccc ctttaggttt cggtgcggtc gtgttcggcg cggcgatacc
     7981 gtttgccgcc ggctggtggg gcagcggctg ggtacacgtc aaactgtgtt tgggcttgat
     8041 gcttttggct taccagttgt attgcggcgt gctgctgcgc cgttttcagg attacagcaa
     8101 tgctttttca caccgctggt accgcgtgtt caacgaaatc cccgtgctgc tgatggttgc
     8161 cgcgctgtat ctggtcgtgt tcaaaccgtt ttgacggagc atcttatgcc gatagaaatc
     8221 gaacgccgtt ttttgattgg aaacgacaac tggcggcaat acgccgacga accgctgctg
     8281 ttgaggcagg gttatctgtc tgttgaaaaa gaacgcaccg tccgtgtccg tattgccgga
     8341 caacgggcgt ggctgacgct gaaaggctat atttcggaac tcagccgcag tgagtttgaa
     8401 tacgaaatcc cgcttgccga tgcggaaaag atgatggaaa cgatgtgtcc gtttaagatg
     8461 gaaaaatggc gttatccggt cagatggggc ggcagcctgt ttgaaatcga tgtttttctt
     8521 ggcgataatg cgcctttggt cgtcgcggaa atcgagttgc ccgacgagaa tgccgatttt
     8581 gaccgtccgg actggctggg acgggaaatc actgccgacg gtatgtttac caatgcctat
     8641 ttgagcaggc atccgttctc aagctggaaa aatgccgtct gaagcggctt cagacggcat
     8701 tctccgacaa ccatcgcaaa tccaaagaca gcctctatat ccttatttct gctttctttg
     8761 ttccattatt cccaataaat ccccataacc taaaatctcg tcattcccgc gcaggcggga
     8821 atccggttct ttaaacttcg gttttttccg ataaattgct ttagcttttc gtttccggat
     8881 tcccgctttt gcgggaatga cgatggaaga ttgttgttat ttcggataaa ttaccctaac
     8941 tttaaaatcc cggattccca tcttcggggg aataacaata ttttctattt cagcccatca
     9001 tttgcggggg attttataga gagtttatat ttcaatatct caaataatgc acaaaagcat
     9061 agctcgtgcc tttgctgctg acacggcgtt ccgtccgctc tgcttctttc caatgcgtcc
     9121 ggtctatttc ggggaaaaac gcatctcctt ccaccgacaa atccacttcg gttatccgca
     9181 aatcggtcgc caatggcatt gcttggtcgt atatctgcgc gccgcccata atgacggctt
     9241 cttccgcgcc tgcgcacaat gccaatgccg cctccaaact tgccgccgtt tccgcacctg
     9301 ccgcgcaata atccgcctgc ctgctgatga cgatgttcct ccgtccgggc aggggtttga
     9361 cgtgcaggga ttcccacgtt ttccgcccca taatgacggg tttgcccaag gtataggctt
     9421 tgaaaaatgc aaaatcttcg gggatgtgcc aaggcatagc attgtccgcc ccgatgcaca
     9481 ggttttccgc acacgccgca attagggtta ttttgagcat atttcctcca caaaaatgcc
     9541 gtctgaacgc ttcagacggc attttatgac attctgcgtc aaaaatctca accggcgcgc
     9601 gccatacgtt ttttgtttgc acctgccaac aatgccaaca gttcttcagt cgcgtcccaa
     9661 ccaatacacg catcggtaat gcttttgccg tacacttccg gcttatcctg tctgccttcg
     9721 accaaatggc tttccaccat cacgcccata atattgctcc cgtcctgttc caattgggca
     9781 gcaatatctt gtgccacttc catctgccgg gtgtaatcct tgcggctgtt ggcgtggctg
     9841 caatctatca tcagcttatc ggttacccct gccgcacgca gttgttccgc cgcctcgctg
     9901 acgtgtcccg catcataatt cggctctttt ccgccgcgca aaatgacatg acagtcggga
     9961 ttgccgccgg tatggacaat ggcggaatgt ccggccttgg ttacagacaa gaaatgatgc
    10021 gaatggctcg ccgcaccgat tgcgtcgatg gcaatcttca aattgccgtc cgtaccgttt
    10081 ttaaagccga cggggcagga cagcccgctt gccaattcgc ggtgaacttg gctttcggtc
    10141 gtccgcgcac cgattgcccc ccaagaaatc aagtccgcat aatattgcgg cgtaatcata
    10201 tccaaaaact cggtagaggc aggcataccc atattgttca gcgacaacaa caggctgcgc
    10261 gcctgacgca aaccgaaatt gatatcgaac gtaccgtcca aatgcgggtc gttaatcaaa
    10321 cctttccaac ccaccgtcgt cctcggcttc tcgaaataaa cgcgcatcac aatcaaaagc
    10381 tcgttttcat actgcttgcg gagtttcaac aaacgctccg catattccaa cgccgctttc
    10441 ggatcgtgaa tcgaacacgg cccgataata accaacagcc gcttgtccct gccgtgaacc
    10501 aaatcggaaa tttcctgacg ggtgcggtga accaagcccg aagcctcttt ggaaatcggc
    10561 agctcgtaaa gatgggcaat cggcggcaac aactctttaa cttctttaat cttaatatcg
    10621 tctgtggggt aatggtgtgt catcttgcat ctttcctgat ggttttttat acgcggctta
    10681 ggttacatcg tttaccttgc acaaacaagt gcaacatatt actacaaagc tattttattt
    10741 caaaataact gatatatctt ttttcataga ttttaaattt catattttta atattttagg
    10801 aattttattc caaagaaaaa gaaacagatt gataaaaaca atacccgcca ttcattcgga
    10861 caaaaccgca cggaacatcc gttttcccat ttccacgaca atccggtaca gaacaccccg
    10921 ctgccgattc cgcctgcaca tccggcaaaa tttcatacgc aaggaaaatg ttgaaaacaa
    10981 aaagaactaa ttgcttgaaa aaagatttaa ttggaatgcg ggtatttccc acactttctc
    11041 catttattca tcctcattat aggaagaaaa acatgaaaaa accctgcttg cactctcact
    11101 tgcagccgtg tccgccgtta cggtagccga tattcctgag tctaacggca agggcaaagc
    11161 catcatcggc gataaggaaa aacaccgtta ccaagcatga aaaccttgtt ttgaaagtct
    11221 atggcgaaat ccgccaagat ttgggggacg gatgatccct gaccccttgg gcggatgcat
    11281 atgcagattt gcgcggcaaa accaaagtga tgacgaccca gatgtgtgct tcccgcgatg
    11341 tcagcaaaag cgccaaaggt tggagtgtcg gtatcggtct gaatgtaggc aaacagttga
    11401 ccgacagcgt cggtctcgag tttgatccat actaccgtca caaaacaatc tgcaaacccc
    11461 gtgagattgt tttggacggc gacaaaacca aaatgggccg ctccaaatcc aacgagtacg
    11521 gcttccgcgt aaccgcaacg ttctatagtc aattaaaatc aaagtaggac agtagtgcat
    11581 cgtcaaatcg ggcgtaatca gacaaaacgg tttgcagata ccgcttaata ttcgcccaca
    11641 ctttctcaat cgggttgagt tcaggtgaat aaggtgcaag aggcaatacc ttatgtccca
    11701 atttttccgc catttcccgt aagacaccca tacggtgaaa tcgcgcatta tctaaaatat
    11761 ag
//
LOCUS       DAXIEB010000064        11571 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000064 DAXIEB010000000
VERSION     DAXIEB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.064"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(261..1340)
                     /gene="apbC"
                     /locus_tag="ACFOA3_001361"
     CDS             complement(261..1340)
                     /gene="apbC"
                     /locus_tag="ACFOA3_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6898359.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSNGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGATAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1551..1626
                     /locus_tag="ACFOA3_001362"
     tRNA            1551..1626
                     /locus_tag="ACFOA3_001362"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1584..1586,aa:Lys,seq:ttt)
     gene            1705..2214
                     /locus_tag="ACFOA3_001363"
     CDS             1705..2214
                     /locus_tag="ACFOA3_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6898360.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2557..2997
                     /gene="nadR"
                     /locus_tag="ACFOA3_001364"
     CDS             2557..2997
                     /gene="nadR"
                     /locus_tag="ACFOA3_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6898361.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3112..3612
                     /gene="hpaC"
                     /locus_tag="ACFOA3_001365"
     CDS             3112..3612
                     /gene="hpaC"
                     /locus_tag="ACFOA3_001365"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6898362.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            3630..4325
                     /gene="murU"
                     /locus_tag="ACFOA3_001366"
     CDS             3630..4325
                     /gene="murU"
                     /locus_tag="ACFOA3_001366"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6898363.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSMQPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4571..5002)
                     /locus_tag="ACFOA3_001367"
     CDS             complement(4571..5002)
                     /locus_tag="ACFOA3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6898364.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            5177..6853
                     /locus_tag="ACFOA3_001368"
     CDS             5177..6853
                     /locus_tag="ACFOA3_001368"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6898365.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEDF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6968..8059
                     /gene="ychF"
                     /locus_tag="ACFOA3_001369"
     CDS             6968..8059
                     /gene="ychF"
                     /locus_tag="ACFOA3_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6898366.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVELCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8329..10197
                     /locus_tag="ACFOA3_001370"
     CDS             8329..10197
                     /locus_tag="ACFOA3_001370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6898367.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLIGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFAPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQAGFLAALCALSLATSFIPSALDKYYLPHLRACEMLVGSLTAVWMRCRQPAV
                     GRRCAAAGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCI
                     EKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYVSR
                     VFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            10259..11554
                     /gene="tyrS"
                     /locus_tag="ACFOA3_001371"
     CDS             10259..11554
                     /gene="tyrS"
                     /locus_tag="ACFOA3_001371"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6898368.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2602 a         3096 c         3185 g         2688 t
ORIGIN      
        1 aaatcgtcag aaatacccga accgtcattc ccgcgcaggc gggaatccag acctgtcggt
       61 gcagaaactt atcgggtata acggtttctt gagattttac gttctagatt cccactttcg
      121 tgggaatgac ggcatcggtc tgcctttaca acacggtttc ttagattcca cgttctagat
      181 tcccgcctgc gcgggaatga cggcatatcg gcaggcgcgg ggcttgggac ggcattgctg
      241 ttgcggtttc ggacgcggct ttattcgacg acgattttag ggaaccggct gctgaagtct
      301 ttgcctttgt cggcaatgcc cagggcgatt tggaatgcgg catcggtgta gattcgggcg
      361 atggcggggt gttcgtcgaa cagttgcgcc gtcgcccctc cgtccatggc ttcgcgcacg
      421 ggcaggctta atggaagctg tcccagcagg gggacgttga ggcgtgccgc caagtctttg
      481 ccgccgtctg tgccgaacag tgcttcgctg tgtccgcagt tggagcagat gtggacggac
      541 atattttcca atacacctaa aatgggaatg ttgacttttc ggaacatatc cacggctttg
      601 cgcgcgtcta tcagggcgat gtcctgcggc gtggttacga tgaccgatcc ggttacgggg
      661 atgcgttggg acagcgtgag ttggatgtcg cccgtgccgg ggggcaggtc gataaacagg
      721 tagtccactt cgtcccactc gctttgaaac atcagttgct gcaaggcttg gctgaccatc
      781 ggcccgcgcc agacgacggc ttggtcggta tcgacgagaa agccgataga catgacctgt
      841 atgccgtttg aagattcgac aggaatcagt ttttggtttt tctggtcggg tttgcggtcg
      901 tccacaccca acatggtcgg ttggctcggg ccgtaaaggt cggcatcgag cacgccgacg
      961 cgtgcgccca tgcgcgccat tgcggcggca aggttggcag tggtggttga tttgcccacg
     1021 ccgccttttc ccgatgcgac ggcaatgatg tttttcacgc ctttgatggt ggtaacgccg
     1081 ggctggactt tgtgtgtgcc gatttcagtg tccatggaca gatggatgtg tgtgtcgcct
     1141 gtttcgggca tcagggtttc ttgtacggaa tcggcgatgg cggcggcaat gtgcgcgacg
     1201 gggaagccga aatgcagggc gatatggatg ccgtctgaac gctgttcgac cgaacggacg
     1261 gccttttcgc cgccgagcgt gcgtgccgta ttcggaacgg cgacggtgtc gaggagggtg
     1321 cggatgtttt gtatgttcat aatgtgtgtt tctgtgtgca gtttgggaaa cgggcggaaa
     1381 gtgtacattg atttcgggcg gtttgccttt ccttgtgcga acttttacac ggtatgcggc
     1441 ggattgtgaa aatccgttgt caaaatatcc cgtgcaaaca gtggtctgca attaagcctg
     1501 taaaatttgc ttgacgaggt aggatggtgc tgtataatgc acggcttatc gggtcgttag
     1561 ctcagccggt agagcagcgg acttttaatc cgttggtcga gcgttcgaat cgctcacgac
     1621 ccaccagata accggaagcc aagttttgag acttggcttt ttgtttgcct gccggttgcg
     1681 cgggcgtacc gtttgagggg gcgtatgaaa aagaaactgc tttcgggcat caaatttgcc
     1741 gttcagacgg cattggtgtt tttattggtg tcgctgtttt tggattggat acgcaagccc
     1801 gacgaacctg ccggtgcggc agggcggcct ttgaccctgc tgtcggggca gcggctgact
     1861 ttgggacagt ttagccggga taaggcagtt ttggtgtatt tttgggggag ctggtgcggc
     1921 gtgtgccgtt atcagtcgcc gataatcgat gatttggcgg cggacggcgt tccggtcgtc
     1981 ggcgtggcgg tacgttccgg cagcgcgtcc gaagtggcgg cgtatatggc aaagcggggc
     2041 ttgggctttc cgacggtcaa cgatgaggac gggggtttgg caaggtcttg gcggattgtc
     2101 gcaacgcctg ccgtcgtttt ggtcaaaaat gggaaaatgg ttcactatac gacgggaatc
     2161 agcagttatt ggggcttgcg cgcacgaatt tttcaggcgg atgttttcgg ttaacctttg
     2221 tttttgttaa actttacgct atgtagagaa tcaaacggca tataccttgc tcgtctgatg
     2281 cggcggcttt tgttttttct ggaagaaagt tctttcgaca gtaaatgtaa tactttgttg
     2341 agattgtagg gaaaatagac taagatataa ctattaaaat atttttagat aggattatcg
     2401 gaattaaagt cttttatacc cagtcgtccg atgcggttta tagcgtattg ttgctatatg
     2461 ttcgttttgt tatataacgg ttgcatcaaa atttacgccc acaggctttc ccgacggttt
     2521 gaaagtttga ttttcgataa cttggagact tgaacaatgc ctacccaatc aaaacatgcg
     2581 tctatcaata tcagtctgat acaggcaagg gaagccctga tgacccaatt caggcctatt
     2641 ctgaatcagg cgaatattac cgatcagcaa tggcggatta tccgtctttt ggcggaaaac
     2701 ggcacgctgg actttcaaga tttggcgaat caggcgtgca ttttgcgccc cagcctgacc
     2761 ggtatcctga cccgccttga aaaagcgggt ttggttgtcc gcctgaaacc ttccaacgac
     2821 caacgacgtg tttttctgaa gctgactgcc gagggcgaga agctgtatga ggaaatcggc
     2881 gaagaagtgg acgagcgtta cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg
     2941 ctgcttaaag acctgttggc agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac
     3001 gccgtaacgc gcggaaatgt ccgaccgacg gctttttgaa tcagaactgc tgcacatggg
     3061 gggtgccttg tgtgcagcat tcttatatag gggacagttt aaaggggaaa aatggcggat
     3121 ttgcagaaaa cttttcaaac ttcgttccgt gatgcgatgg catcttgtgc ggcaggcgtt
     3181 catgtcatca cgacagacgg tgcggcaggg cgttacggca ttacaatgac ggcggtcgcg
     3241 ccggttaccg acgagccgcc gaccgtgatg ctgtgcatca accggagtgc gcgaatcatt
     3301 ccgatcctgt cggaaaacgg cagcctctgc atcaatacgc tggcggacga acatcaggat
     3361 gttgccgaac attttgccgg gctgaccggc ctgtcgcccg aagagcggtt tgcctaccac
     3421 atctggcatc gcggcaaaac gggacaactt gaaatagagg gcgcgttggc gcacctgcac
     3481 gggcatattg tcggtcaaca tgaaatcggc acgcattttg tgttttacgt caggctcgac
     3541 gaaattaaaa attgcgtggg caaacgtccc gcgctgctgt atttcagacg gcagtttaga
     3601 tctttagact gatattcgga cagatatata tgaaagcgat gatactggcg gcaggacgcg
     3661 gcgagcgtat gcgccctttg accgatacca ctccgaagcc gctgcttgat gtggcgggta
     3721 agcctctaat cggttggcac ttatgccgtc tgaagcaggc ggggtttacc gaaatcgtca
     3781 tcaaccacgc ttggctgggt cggcagatag aagatgcttt gggcgacggc tcggcttatg
     3841 gcgtgaacat cgcctattcg cccgaacccg caggcggttt ggaaacggca ggcggcatcg
     3901 cgcaggcatt gccgctgttg ggtgggcagc cgtttttggt ggtcaacggc gacgtgctga
     3961 ccgacatcga ttttaccgcc gcgtttcaga cggcatcgtc cctgccggaa catatttccg
     4021 cccatctgtg gctggtggaa aatcccccgc accatcctga cggcgatttt tccctgctgc
     4081 ccgacggcag catgcagccg gaagtaaatg gcggcaacgg attgacattt agcggcgtgg
     4141 gtatttaccg tcctgaaatg tttgacggaa tcgaagcggg cagtgtggcg aaactcgcgc
     4201 ccgtattgcg gaacgaaatg cggcaaaacc gcgtgagcgg acagaagcat acgggcttgt
     4261 ggctggatgt cggcacggta tgccgtctga aggaagcgca agcccttgca ggggcttgga
     4321 agtgaaataa aacggtggct tgccgagccg gctgccgaac ggttccgacc ggatgtccag
     4381 catattttcg tggaggcgtg aaaatagggc gttcggggct ttccgtccaa caaatgccat
     4441 ctgaagccga cccctgtcgg atgcggcggt ttgccgtcgg cgcggtgtcg gattttattg
     4501 ggtaactttg ttcagaccgc cgcccgtcag attgtcaaaa cccggtttca ggcggtatgg
     4561 cggattcggt ttaacgtttc aacgccagca ccaacacgcc cgccgttacc agccccaagc
     4621 ctatccattc ctgcgtgctg gggcgttcgt tgaggaagac tactgccatc agggcgacca
     4681 gaaccaggct gaatttatcg acgggggcga cttgcgaggc gttacccagt tgcagggctt
     4741 tgaagtaggc aagccaagat gcgccggtag caaggccgga taggataagg aatgtccagt
     4801 tgtgccccgt aaagccgttc acgccctgcc atttgccggt gtaggttaaa aacaataaca
     4861 aagcggcgag gatgaccaag gtgcggatga aggtagcgaa atctgaatct ataccctgta
     4921 aacccatttt ggcgaagatg gcggtcaatg aggcgaagcc tgccgatgcc aatgcccaaa
     4981 acagccatgc gttgctgctc atgttttctc cttggattgt gaacaatatg aacggtattt
     5041 ttgttgcagc gtcaaaaatt tcactgcggg tttggtgcgg ataacgttat aatatgcctg
     5101 atattatttt caatccacct gtttgtcgcc tgatgctttc agacggcatg tccctcctca
     5161 tttctaaagg aaaatcatga gcttcaaaac cgatgccgaa atcgcccaat cctccaccat
     5221 gcgcccgatt ggcgaaattg ccgccaagct tggtctgaat gccgacaaca ttgagcctta
     5281 cggtcattac aaggcgaaaa tcaatcctgc cgaagcgttc aaactgccgc aaaaacaggg
     5341 caggctgatt ttggttaccg ccatcaaccc gactccggcg ggcgaaggca aaaccaccgt
     5401 aaccatcggt ttggcggacg cgttgcgcca catcggcaaa gatgccgtga ttgccctgcg
     5461 cgaaccttct ttagggccgg tgttcggcgt gaaaggcggc gcggcaggcg gcggctatgc
     5521 ccaagttttg ccgatggaag acatcaatct gcactttacc ggcgacttcc acgccatcgg
     5581 tgcggcgaat aacctgctcg ccgccatgct cgataaccat atctaccaag gcaacgagtt
     5641 gaacatcgat ccgaaacgcg tgctgtggcg gcgcgtggtc gatatgaacg accgccagtt
     5701 gcgcaacatc atcgacggca tgggcaaacc tgttgacggc gtgatgcgtc ctgacggttt
     5761 tgatatcacc gttgcttccg aagtgatggc ggtattctgc cttgccaaag acatcagcga
     5821 tttgaaagag cgtttgggca acatccttgt cgcctacgcc aaagacggca gccccgttta
     5881 cgccaaagat ttgaaagcac acggcgcgat ggcggccctg ctcaaagatg cgattaagcc
     5941 caatttggtg caaaccatcg aaggcacgcc cgccttcgta cacggcggcc cgttcgccaa
     6001 catcgcccac ggctgcaact ccgtaaccgc aacccgtctg gcgaaacacc ttgccgatta
     6061 cgccgtaacc gaagcaggct tcggcgcgga cttgggcgcg gaaaaattct gcgacatcaa
     6121 atgccgcctt gccggtttga aacctgatgc ggctgttgtc gtggcgactg tccgcgcttt
     6181 gaaatacaac ggcggcgtgg aacgcgccaa cctcggcgaa gaaaatttag acgctttggc
     6241 aaaaggcctg cctaatctct tgaaacacat ttccaacctg aaaaacgtat tcggactgcc
     6301 cgtcgtcgtt gcgctcaacc gcttcgtgtc cgactccgat gccgagttgg cgatgattga
     6361 aaaagcctgt gccgaacacg gcgttgaagt ttccctgacc gaagtgtggg gcaaaggtgg
     6421 tgcgggcggc gcggatttgg cgcgcaaagt cgtcaacgcc attgaaagtc aaaccaataa
     6481 cttcggtttc gcctacgatg tcgagttggg catcaaagac aaaatccgtg cgattgccca
     6541 aaaagtgtac ggcgcggaag atgttgattt cagcgcggaa gcgtctgccg aaatcgcttc
     6601 actggaaaaa ctgggcttgg acaaaatgcc gatctgcatg gcgaaaaccc aatactcttt
     6661 gagcgacaac gccaaactgt tgggctgccc cgaagacttc cgcatcgccg tgcgcggcat
     6721 caccgtttcc gcaggcgcag gtttcatcgt cgccctgtgc ggcaacatga tgaaaatgcc
     6781 cggcctgccc aaagttccgg ctgccgagaa aatcgatgtg gacgcagaag gcgtgattca
     6841 cggcttgttc tgaacggttt tctgaaaccg gatgccgtct gaagccgttt cagacggcat
     6901 tttttcggaa cgcgggcggc ggtatgctat aatccgccgt taaatttctc tattttcagg
     6961 aaaaaacatg agtttgaaat gcggcatcgt cggtttgccc aacgtcggca aatccaccct
     7021 ttttaacgcg ctgacccaat cgggtatcga agcggcaaac tatcctttct gtaccatcga
     7081 acccaacgtc ggcatcgtcg aagtccccga tccgcgtatg gccgaattgg caaaaatcgt
     7141 caatccgcaa aaaatgcagc ctgccatcgt cgaatttgtc gatattgccg gtttggttgc
     7201 aggcgcgagc aaaggcgagg gcttgggcaa ccagttcctt gccaacatcc gcgaaaccga
     7261 tgcgattgtg aatgtcgtgc gctgctttga cgacgacaac atcgtccacg ttgcaggccg
     7321 cgtcgatccg attgccgaca ttgaaaccat cggtacagag ttggcccttg ccgacttggc
     7381 aagtgtggaa aaagccatcg tccgcgaaga aaaacgcgcc cgttccggcg acaaagacgc
     7441 gcaaaaactg gtcgaactgt gtaaaaaact gctgccgcat ctggacgaag gcaaacccgt
     7501 gcgttccttc ggtttggacg cggaagaacg cgcgatgctc aaaccactgt tcctgctgac
     7561 cgccaaaccg gcgatgtatg tgggcaacgt cgccgaagac ggttttgaaa acaatccgca
     7621 cctcgaacgc ctgaaagaat tggcggcaaa agaaaacgcc cccgtcgtcg ccgtttgcgc
     7681 cgcaatggaa agcgaaattg ccgaattgga agacgacgaa aaagccgaat tcctcgccga
     7741 aatgggcttg gaagaacccg gcctgaaccg cctgattcgt gccggttacg acctcttggg
     7801 gctgcaaacc tatttcaccg ccggcgtgaa agaagtccgc gcgtggacga tacacaaagg
     7861 cgacaccgcc ccgcaagccg ccggcgtgat tcacaccgac tttgaacgag gcttcatccg
     7921 cgcccaagtc atttcttacg acgactttgt ctcgctcggc ggcgaagcca aagccaaaga
     7981 agccggcaaa atgcgtgtgg aaggcaaaga atacgtcgtg caagacggcg atgtaatgca
     8041 ctttttgttt aacgtgtaac ccaaatgcgg caggttttca gacggctttg ccggaaatgc
     8101 cgtctgaagc cggttttggt ggttttcggc gtttccatac cgccggaatg cggccgcatc
     8161 aaaataaaat cccgcccgca tttccgattt gccctccccg attcctgcaa aacaaaccgc
     8221 ctgccccgct gttgcaaaaa tccgtccggc attccgaata tcccgagccc cgatacaaaa
     8281 tgacctttca gatggcattt gcgccgccgc gatgaaagca aaaatattat gagccaagcc
     8341 ttaccctacc gcccggacat cgacacattg cgcgccgccg ccgtcttgtc cgtcatcgtg
     8401 ttccatatcg aaaaaaattg gctgccgggc ggatttctcg gtgtcgatat attctttgtg
     8461 atttcaggct ttttgatgac ggcgatcctc cttcgcgaaa tgtcgggggg ggggaggttt
     8521 tccctgaagg cattttatat ccgccgcatc aaacggattt tgcccgcatt tttcgccgta
     8581 ttggcggcaa cgctgatagg cggcttcttt ttattcacca aagatgattt ctttctttta
     8641 tggaaatccg cgctgaccgc cttgggtttc gcatccaacc tgtattttgc aaggggcaag
     8701 gactatttcg ctcccgcgca ggaagaaaag cccctgctgc acatttggtc tttgtcggtc
     8761 gaagaacaat tttactttgt ctttccgata ttgctgctgc ttgtcgcccg caaaagcctg
     8821 cgcgtccaag ccggcttcct cgccgcactg tgcgcgttaa gccttgccac ttcctttata
     8881 ccttccgcgc tcgataaata ttacctgccc cacctgcgcg cctgcgaaat gctggtcgga
     8941 tcgctgaccg ccgtgtggat gcgctgccgg caacctgccg tcggcagacg ctgtgccgcc
     9001 gccggcgcat tgtttgccgt gtgcatattg tcaacctgcc tgttttccta ttcggaacaa
     9061 accgcctatt tcccgggccc cgccgctttg attccctgtc tggctgttgc cgcgctgatt
     9121 tatttcaatc attacgaaca cccgcttaaa aaatttttcc aatggaaaat caccgttgcc
     9181 gccggtttga tttcctattc gctttatctg tggcattggc cgatattggc ctttatgcgc
     9241 tatatcggcc cggacaacct gccgccttat tcgccggcgg cagcaatcgt cctgactctg
     9301 gcgttttccc tgatttctta tcactgcatc gaaaagccgt ttaaaaaatg gaaaggttcg
     9361 ttcgcacaat ccgttttatg gatttatgcc ttgcctatgc tcattttggg ggcgggctcg
     9421 ttttttgcga tgagactgcc gtttatggcg caatacgacc gcttggggct gacgcgttcc
     9481 aacacctcct gccacaacaa taccggcaaa caatgcctgt ggggggatac ggaaaaacag
     9541 ccggagctgc tggttttggg cgactcccac gccgaccatt acaaaacatt cttcgatgcc
     9601 gtgggcaaaa aagaaaaatg gtccgccact atggtttccg ccgacgcttg cgcctatgtg
     9661 gaaggctacg tgtcccgcgt gttccaaaac tgggccgcct gccgcgccgt ttaccgctat
     9721 gccgaagaac acctgccccg gtattcaaaa gtggttttgg cgatgcgctg gggcagccaa
     9781 atgcccgaaa acagccgctc ccttgcctac gatgccggtt ttttccaaaa attcgaccgt
     9841 atgctgcata aactctcgtc cgaaaaacaa gccgtttacc tgatggcgga caaccttgcc
     9901 tcgtcttaca acgtccagcg cgcctatatc ttgtcttcac gcataccggg ttaccgccaa
     9961 gccctgcgcc cggacgacga aagcaccctg aaagccaatg cacgcatcag ggaattggca
    10021 gccaaatacc ccaacgtcta tattattgat gccgccgcct atatccccgc agattttcaa
    10081 atcggcggat tgccggttta ctcggacaaa gaccacatca acccttacgg cggcacggaa
    10141 ttggcaaagc gtttttccga aaaacaacgg tttctcaata cgcgccataa ccattgattc
    10201 gcttaaattt gttacaatcg gcggtttgca aaaacgctaa tttttttgaa agagaccgat
    10261 gagcgtcatc caagacctgc aatcgcgcga ccttatcgcg caaacgaccg acatcgaagc
    10321 cttagacgct ttgttgaacg aacaaaaaat cgccctttac tgcggtttcg atccgaccgc
    10381 cgacagcctg cacatcggac acctgctgcc cgtattggca ttgcgccgct tccaacaggc
    10441 ggggcatacg ccgattgctt tggtgggcgg cgcgaccggt atgatcggcg accccagctt
    10501 caaagccgcc gaacgcagct tgaattccgc cgaaactgtt gccggctggg tggaaagtat
    10561 ccgcaaccaa ttaacccctt tcttgagctt tgaaggcgga aatgccgcca ttatggcgaa
    10621 caatgccgac tggttcggca gcatgaactg ccttgacttc ctgcgcgaca tcggcaagca
    10681 tttctccgtc aacgccatgc tgaacaaaga atccgtcaaa cagcgcattg accgcgacgg
    10741 cgcaggcatt tccttcaccg agttcgccta ctccctgctg caaggctacg acttcgccga
    10801 gttgaacaaa cgccacggcg cggttttgga aatcggcggc tccgaccaat ggggcaacat
    10861 caccgccggt atcgacctga cccgccgcct gaatcaaaaa caagtgttcg gcctgaccct
    10921 gcctttggtt accaaatcag acggcaccaa attcggcaaa accgaaggcg gcgcggtgtg
    10981 gctgaacgcg aaaaaaacct cgccctatca gttctaccaa ttctggctga aagtcgccga
    11041 tgccgatgtg tataaattcc tgaaatactt taccttcctg tccatcgaag aaatcgacgc
    11101 tatcgaagcg aaagataaag ccagcggcag caagcccgaa gcgcaacgca tcctcgccga
    11161 agaaatgacc cgcctgattc acggcgaaga agcccttgcc gccgcgcagc gcatttccga
    11221 aagcctgttt gccgaagacc aaagcagcct gaccgaaagc gacttcgagc agctcgccct
    11281 cgacggcctg cctgcatttg aagtttcaga cggcatcaat gtcgtcgaag ccttggtcaa
    11341 aaccggtttg gcatcctcca ataaagaagc gcgcggcttt gtgaacagca aagcggtttt
    11401 gctcaacggc aaacctgccg aagccaacaa ccccaaccac gccgccgaac gccccgacga
    11461 tgcctgcctg ctgaacggcg aacacaaacg tttcggcaaa tacaccatcc ttcggcgcgg
    11521 caaacgcaac cacgcgcttt tggtttggaa ataatccgat tgccgcagaa a
//
LOCUS       DAXIEB010000065        11443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000065 DAXIEB010000000
VERSION     DAXIEB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.065"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            261..1382
                     /gene="dnaJ"
                     /locus_tag="ACFOA3_001372"
     CDS             261..1382
                     /gene="dnaJ"
                     /locus_tag="ACFOA3_001372"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6898369.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1578..3596
                     /locus_tag="ACFOA3_001373"
     CDS             1578..3596
                     /locus_tag="ACFOA3_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6898370.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(3639..4196)
                     /gene="rfbC"
                     /locus_tag="ACFOA3_001374"
     CDS             complement(3639..4196)
                     /gene="rfbC"
                     /locus_tag="ACFOA3_001374"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6898371.1"
                     /translation="MNIIDTALPDVKILKPQVFTDGRGFFMETFRDGWFKENIADQTF
                     VQENHSQSRKGVLRGLHYQTENTQGKLVRAIAGEVFDVAVDMREGSPTFGKWTGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLPLSR"
     gene            complement(4241..5107)
                     /gene="rfbA"
                     /locus_tag="ACFOA3_001375"
     CDS             complement(4241..5107)
                     /gene="rfbA"
                     /locus_tag="ACFOA3_001375"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6898372.1"
                     /translation="MKGIILAGGNGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITTPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLRQAAAKKHGATVFAYQVKDPERFGVVEFDENFNALSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQDLHIACLEEIAWRNGWLTKKDVETRAKHLEKT
                     AYGQYLLHLIGK"
     gene            complement(5170..6252)
                     /gene="rfbB"
                     /locus_tag="ACFOA3_001376"
     CDS             complement(5170..6252)
                     /gene="rfbB"
                     /locus_tag="ACFOA3_001376"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6898373.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTRDAVVNVDKLTYAG
                     NLESLTEVADSPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIVGTFNLLEAARAYRRQMPSEQREAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYAASKASSDHLVRAWQRTYGLPAIVSNCSNNYGPRQFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVDETYNIGGHNEKANIEVVKTICALLE
                     ELVPEKPAGVARYEDLITFVPDRPGHDVRYAIDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            complement(6253..7269)
                     /gene="galE"
                     /locus_tag="ACFOA3_001377"
     CDS             complement(6253..7269)
                     /gene="galE"
                     /locus_tag="ACFOA3_001377"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6898374.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDRQILRQIFSEHEIESVIHFAGLKAVGESVAEPMKYYGNNVY
                     GSLVLAEEMARAGVFKIVFSSSATVYGNAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLRIPYRIQPRRAGDLACSFADPSHTKQQTGWETKRSLAQMMEDSWRWVSRNPD
                     GYGD"
     gene            7384..9657
                     /locus_tag="ACFOA3_001378"
     CDS             7384..9657
                     /locus_tag="ACFOA3_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6898375.1"
                     /translation="MNITRILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEDRKVVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEATARGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVEGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGCKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDVSAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            9799..10866
                     /locus_tag="ACFOA3_001379"
     CDS             9799..10866
                     /locus_tag="ACFOA3_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6898376.1"
                     /translation="MYSIEQFPPEIDFETVAVLKKLASAHRYLAELKGICRSIPNQGI
                     LINTLSLQEAKDSSEIENIITTHDELFRAGISASPSSPAIKEVQNYASALHCGFGLIR
                     KHGILTNNHILAIQAELEKNRAGFRKQSGTMLKNDRTGETVYTPPQHTDDIIRLMGRL
                     EAFVNDGSTAKPIDPLIRMALIHHQFESIHPFYDGNGRTGRIINVLYLVLTGLLDIPV
                     LYLSRYLVRNKSEYYRLLQHVRDTGEWDGWLLYMLEAVEQTAKDGIDTVQHIHQAMLD
                     YKHRIRKNFSFYSQDLINHLFNHPYTKIDFLMKTLNVSRPSAAKYLDELTEGGFLHKE
                     KIGRSNYYINIALMNILLPTN"
     gene            11005..11334
                     /locus_tag="ACFOA3_001380"
     CDS             11005..11334
                     /locus_tag="ACFOA3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898377.1"
                     /translation="MFAALVSCFSVSAFAACADSRQQCVIYKNGNVAAEGGCTVSKCE
                     DAGAKVLKWKMKNGKGVTVEIGKNGKVSVNKKPGAKAANSNAAGMGLTCYAADADKRE
                     QFCSTNY"
BASE COUNT         2648 a         2897 c         3212 g         2686 t
ORIGIN      
        1 gaagtctgca gccgcatcaa atgccgtctg aacatgcgtt cgggcggcgt ttttataaca
       61 aaaacacttc atggcggttg gttttatgcc tgtctaagtt tttgtgtcgt gcatacctga
      121 agatttcaga cggcatcggt ttatgctgtc tgaaaagtgt attccgtttc agtttgtaag
      181 ctatggcagt ctgtttgtct tgtgttttgc gcaattgccc ttattttgag ccgtgatttt
      241 attttgaatt agatgaaaaa atgagtaatc aagattttta tgcgacgctg ggtgtggcaa
      301 gaacagctac cgatgatgag attaaaaaag cctaccggaa attggcgatg aaataccatc
      361 ccgaccgcaa tcctgacaat aaagaggcgg aagagaagtt taaagaagta caaaaggcgt
      421 atgaaacttt gtccgacaag gaaaagcgcg ctatgtacga ccagtatggt catgcggcgt
      481 ttgaaggcgg cggacagggg ggcttcggag ggtttggcgg atttggcggt gcgcagggtt
      541 ttgactttgg ggatattttc agccaaatgt ttggaggcgg ttcggggcgc gcccagcctg
      601 attatcaggg tgaggacgtt caagtcggta tcgaaatcac gcttgaagaa gccgcaaaag
      661 gtgtgaagaa acgcatcaat attccgactt atgaagcgtg tgatgtctgt aacggcagtg
      721 gcgcgaaacc ggggacatcc ccggaaacct gcccgacttg caaaggttcg ggtacggtgc
      781 acatccagca ggcgattttc cgtatgcagc agacttgtcc gacctgccac ggtgcgggca
      841 aacacattaa agaaccttgc gtcaaatgcc gtggcgcggg gcggaataag gcggtcaaga
      901 cggtggaagt caatattccc gccggtatcg atgacgggca gcgtatccgt ttgagcggcg
      961 aaggcggacc gggtatgcac ggtgcgcctg ccggcgacct gtatgtaacc gttcgcattc
     1021 gggcgcataa gattttccaa cgcgacggtc tggacttgca ttgcgaactg ccgatcagtt
     1081 ttgccacggc tgctttgggc ggggagttgg aagtgccgac cttggacgga aaggtcaagc
     1141 tcaccgtccc caaagaaacc caaaccggca ggaggatgcg cgtgaagggt aagggtgtca
     1201 aatctttacg cagcagcgcg accggcgatt tgtactgcca tattgttgtc gaaacgcctg
     1261 tcaatttgac cgaccgtcaa aaagagcttt tggaagaatt tgagcggatt tctaccggct
     1321 tggaaaacca aacaccgcgc aagaaatcgt ttttagacaa gctgcgcgat ttgtttgatt
     1381 gattttaagg ttcggaaaca agcagccgta tcggggaatc tccctgatat ggctgttttt
     1441 gtttgtttaa aaatagtttt tattttcaat ggggtatggg gcagggtggg ataactgttt
     1501 ttaactgttc tttttaaaac ttgacatcat ggcgcgatgc caacaatatg cgggaatctg
     1561 ttgtcaaagg aagaataatg aataaatctt tatccggttc ggtagaagaa taccgcgaac
     1621 tgacgctccg aggcatgata ctcggtgcat tgattactgt aatttttact gcgtccaacg
     1681 tttacctcgg tttgaaagtc ggactgacct ttgcctcgtc gattccggcg gcggtaattt
     1741 cgatggcggt tttaaagttt ttcaaaggca gcaatatttt ggaaaacaac atggtgcaga
     1801 cccaagcttc ggcggcgggt acgctttcga ccatcatctt cgtcctgccc ggtttgctga
     1861 tggcgggcta ctggagcggt ttcccgttct ggcagacgac gcttttatgt attgccggcg
     1921 ggattttggg ggtgattttc accattccgc tgcgttacgc gatggtggtg aaaagcgatt
     1981 tgccttatcc ggaaggtgtg gcggctgctg aaattttgaa agtgggcggt catgaagaag
     2041 gggataaccg tcagggcggc agcggcatca aagagctggc ggccggcggt gcgttggcgg
     2101 gattgatgag cttttgcgcc ggaggtctgc gtgtgattgc cgatagcgcg agttattggt
     2161 ttaaaagcgg tacggcaatt ttccaactgc cgatgggctt ttcactggca ttgttgggcg
     2221 cgggctattt ggtcggactg acgggcggta tcgccattct gttgggcatt tcgattgctt
     2281 ggggcattgc cgtgccgtat ttttcatcgc acatcccgca gccttccgat atagaaatgg
     2341 cggcgtttgc gatgaagctg tggaaggaaa aagtacgctt catcggcgcg ggaaccatag
     2401 gtatcgcggc ggtttggacg ctgttgatgc tgctcaagcc gatggtggaa ggcatgaaga
     2461 tgtcgttcaa gagttttggc ggcggtgcgc ccgctgcgga acgcgccgaa caggatttgt
     2521 cgcctaaagc catgattttt tgggtgctgg cgatgatgtt tgttttaggc gtgtcgtttt
     2581 accactttat cggcgattcg cacattacgg gcggcatggc ttggcttttg gtggtcgttt
     2641 gcacgctttt ggcttccgtc atcggctttt tggtcgccgc cgcctgcggt tatatggcag
     2701 gtttggtcgg ctcgtcttcc agcccgattt ccggcgtggg catcgtgtcc gtcgtcgtta
     2761 tttcactggt tttgctgctg gtaggcgaat ccggaggttt gttggcggat gaggctaacc
     2821 gcaaattttt gctggcactg actttgtttt gcggctcggc agtaatctgc gtggcttcga
     2881 tttccaatga caacctgcaa gacttgaaaa ccggctacct gctcaaagcc acgccttggc
     2941 ggcagcaagt cgccctgatt atcggctgta tcgttggtgc gctggttatt tcgcccgtgt
     3001 tggaactgct ttacgaagcc tacggcttta ccggcgcaat gccgcgcgaa ggcatggatg
     3061 cggcgcaggc tttggcagca cctcaagcga ctttgatgac gaccatcgcg tcgggcattt
     3121 tcgcccacaa cctcgaatgg gtctacatct ttaccggcat cgcgattggc gcggtgttga
     3181 ttgccgccga tttggtgttg aaaaaatcat caggcggcaa acttgccctg cccgtccttg
     3241 cggtcggtat gggtatttat ctgccgccgt ccgtcaatat gcccatcgtg gcaggcgcgg
     3301 tgttggcggc ggtgttgaaa cacatcatcg gtaaaaaagc ggaaaaccgc gaaggccgtc
     3361 tgaaaaacgc cgagcgtatc ggaaccttgt tctccgccgg cctgattgtc ggtgaaagcc
     3421 tgattggtgt gattatggcg tttattattg ccttctccgt gaccaacggc ggctcggatg
     3481 cgccgcttgc gttgaatctg caaaactggg atgccgccgc ttcttggttg ggtttggcgt
     3541 tcttcgttac tgggatgttt ttctttgcac agcgcgtact gaaggcgggc aagtaggctg
     3601 tcggaaaaaa tgccgtctga agcgttcaga cggcattttt atctggaaag cggaaggcgg
     3661 agcttttcgg cttgcgccca cgttttgccg gcaaggtctt tgggcgacag cagcggcgcg
     3721 gtttgaagcg gccagcctat gccgactgtc gggtcgttcc atattaaaac ctgttcggct
     3781 tcaggcttgt aatagtcggt gcatttgtac acgacttccg ccgcgtcgcc caaaacgcaa
     3841 aacccgtgcg cgaagccttc gggtatccaa agctggcatt ggttttgcgc ggacagggtt
     3901 gcgcccgtcc atttgccgaa agtcggcgaa ccttctcgca tatcgacggc cacgtcgaac
     3961 acttctccgg caattgcgcg tacgagtttg ccttgggtgt tttcggtttg gtagtgcagg
     4021 ccgcgcaata cgcctttgcg tgattgggaa tggttttcct gcacgaaggt ttggtcggca
     4081 atattttctt tgaaccatcc gtcgcggaag gtttccataa aaaaaccgcg cccgtctgtg
     4141 aagacttgcg gttttaaaat ttttacgtcg gggagggcgg tgtcgatgat gttcatgatt
     4201 ttctctgtat gccgtctgaa gaaagtggtt cagacggcat ttatttgcca atcaggtgca
     4261 gcaggtattg cccgtaggcg gttttttcca aatgttttgc gcgtgtttct acatcttttt
     4321 tcgtcagcca gccgttgcgc caagcgattt cttcgaggca ggcgatgtgc aggtcttgga
     4381 tgttttgcac ggtttggacg aatgaagcag cctcgtgcag gctctcgtgg gtgccggtgt
     4441 ccaaccatgc gaaaccgcgc cctaagattt ggacggacaa tgaaccgtct tccaaataca
     4501 tttggttgag gtcggaaatt tccaattcac cgcgtgcgga cggtttgagc tgtttggcga
     4561 actcgacggc gcggttgtcg tagaagtaca agccggttac cgcccaatcg gatttgggtc
     4621 gttgcggttt ctcttcgata gacagggcgt tgaaattttc gtcaaattca accacgccga
     4681 aacgctcggg atctttgacc tgataagcaa acacggttgc gccgtgtttt tttgctgccg
     4741 cctgccgcaa tgtttgcgta aacgattggc cgtagaaaat attgtcgccc aaaaccaagc
     4801 aaacattgtc gttgccgata aattcttcgc cgatgataaa tgcctgtgcc aagccgtcag
     4861 ggctgggttg aacggcatag ctgatggaaa tgccgaaatc gctgccgtcg ccgagcaggc
     4921 gtttgaaaga ggcattgtct tcgggcgtgg taatcaccaa aatatcgcgg attcctgcca
     4981 gcatcaaaac cgacaagggg taataaatca tcggtttgtc gtacacgggc aggagctgtt
     5041 tggacacgcc gcgtgtgatg gggtagaggc gcgtgccgtt gccgcctgcc agtatgatgc
     5101 ctttcatctt tgctttcttc ctttgcgatg ggttttcaga cggcattgcg tcgggatgcc
     5161 gtctgaaaac tatttgcctg tacctaaacg ttccaaacga tagctgccgt tcaatacatt
     5221 ttgccaccag gttttgttgt ccagatacca ttgcacggtt ttgcggaggc cggactcgaa
     5281 ggtttccaaa ggtagccagc ccaaatcccg cctgattttg gctgcgtcga tggcatagcg
     5341 cacgtcatgg ccggggcggt ccgggacaaa ggtaatcaaa tcttcataac gtgccacacc
     5401 cgccggtttt tcaggaacaa gctcttccag cagggcgcag atggttttga cgacttcaat
     5461 attggctttt tcattgtggc cgccgatatt gtaggtttcg tcgacaacac cttcggttac
     5521 aacctgatac agcgcgcggg catgatcttc gacaaacagc cagtcgcgga tttgcatacc
     5581 gtcgccgtac acaggcagcg gtttgccgtc aagcgcgttc agaatcatca aaggaatgag
     5641 tttttcgggg aattgtcggg gcccgtaatt attggaacag ttgctgacaa tggcgggcag
     5701 cccgtaagtc cgttgccacg cgcggacaag atgatcggaa gatgctttag acgcggcata
     5761 aggactggac ggcgcgtagg gcgcggtttc ggtaaacaaa tcgtctgtgc cgcctaaatc
     5821 gccatagact tcatcggtgg aaatatggtg gaaacggaag gcttcgcgct gttcagacgg
     5881 catttgccgc cggtaggcgc gcgcagcttc aagcagattg aatgtgccga cgatattggt
     5941 ttggataaac tcgcctgccg aatcgataga gcggtcgaca tggctttccg ccgccaagtg
     6001 catcacggca tcaggccggt attgcgcgaa cacgcggtca agttcggcgc ggtcgcaaat
     6061 atccacttgc tcaaaagcgt agcgtggact gtcggctacc tcggtcaaag attccaaatt
     6121 gccggcataa gtcagcttat cgacattgac gacagcgtcc cgggtgtttc ggataatatg
     6181 gcggacaacg gcagaaccga taaagcccgc gccgccggta acgaggatgg tttttttatt
     6241 tgcggtttgc atttaatccc cgtatccgtc ggggttgcgg ctgacccaac gccacgaatc
     6301 ctccatcatt tgcgcaaggc tgcgcttggt ttcccagccg gtttgttgtt tggtatggga
     6361 cgggtcggca aaggaacacg ccaagtcgcc ggcgcggcgg ggttggattc ggtaaggaat
     6421 gcgcaaaccg gatgcggcct caaaggcacg gatgatttcc aaaaccgaat aggcgcgccc
     6481 cgaacccaag ttgaacaaat gtacgccggc aacgccgcct ttcgccttca ttgccgcgat
     6541 atgcccttct gccaaatcca tcacatgaat gtagtcgcgc attcccgtac cgtcgggggt
     6601 cggataatcg ccgccgaata ccgacagttg cggcagcctg cccgaagcca cttggcagat
     6661 atagggcaaa aggttgttgg gaataccgtt gggctgttcg ccgatcaaac cgctttcgtg
     6721 cgcgccgatc gggttgaaat agcgcaacaa aatcacgctc caacgcggat cggccttttg
     6781 gatgtcggtt aacatccgtt ccaccatcgc tttggacgca ccgtaaggat tagcggtatc
     6841 gcctgggcgc atatcttccg tataggggac tttttccgca ttgccgtaaa cggttgccga
     6901 cgagctgaat acgattttga acacgcctgc acgcgccatt tcttccgcca gcaccaggct
     6961 gccgtaaaca ttattgccgt aatatttcat cggctcggca acgctttccc ccactgcctt
     7021 caaaccggca aaatggatga cggattcgat ttcgtgttcc gaaaaaatct gcctcaaaat
     7081 ctgacggtcg cggatgtcgc cctgataaaa cggtatgttt ctgccggtaa tttgccgaag
     7141 gcgcgggagg acggcggcag acgagttgca cagattatcc agaatcacgg catcgtaacc
     7201 ggattggacg agcgagacgg cggtgtgcga accgataaag ccggtgccgc cggtaatcag
     7261 gacggtcata aacattcctt tcagattgta tcgggcaaag tatatcagta ttatcttcat
     7321 cttttccaaa cgattaccca cattgtgccg tcggctgttc agacggcatc acggaggcaa
     7381 tttatgaaca tcacccgaat cctctcccaa gaactctccg ccaccgccgc gcaaatcact
     7441 gccgccgtcg agcttttgga cgacggcgcg accgtcccct ttattgcgcg ttaccgtaaa
     7501 gaagccacgg gcgggctgga cgacacgcag ctgcgccagc ttgccgagcg gctgcaatac
     7561 ctgcgcgaat tagaagatcg caaagtcgtt gtgttaaaaa gcattgaaga gcaaggcaag
     7621 ctttcagacg acctcagggc gcaaatcgaa gccgccgaca acaaaaccgc gctggaagac
     7681 ctgtatctgc cctacaagcc caaacgccgc accaaggcgc aaatcgcgcg cgaacacggt
     7741 ttacagccgc tggcggacgt gttgcttgcc gagcagccgc aggacgtgga agccaccgca
     7801 cggggctacc tgaacgaaaa cgtccccgac gctaaagccg cactggacgg cgcgcgcgcg
     7861 attctgatgg aacagtttgc cgaagacgcg gaactcatcg gcacgctgcg cgacaagctg
     7921 tggaacgaag ccgaaatcca cgcgcaagtc gttgaaggca aagaaaccga aggcgaaaag
     7981 ttcagcgatt atttcgacca ccgcgaacct attcgtgcga tgcccagcca tcgtgcgcta
     8041 gcggttttgc gcggacgcaa cgaaggcgtg ttgaacatcg cgctcaaata tcagcccgac
     8101 gacacgccga ttacgcagca aagcgaatac gagcaaatca tcgcccgccg cttcaaagtt
     8161 tcagacggct gcaaatggct gcgcgacacc gtgcgcctga cttggcgcgc gaaaatcttt
     8221 ttgtcgttgg aacttgaagc tctcaaccgt ttgaaagaag ccgccgacac cgacgcgatt
     8281 accgtgttcg cccgcaatct caaagacttg ttgctcgccg cgcctgccgg acggctgacc
     8341 acactgggcc tcgaccccgg ctaccgcaac ggcgtgaaat gtgccgtggt ggacgatacg
     8401 ggcaagttgc tggataccgt cattgtctat ttgcatcaag aaaacaatat gttggcaacg
     8461 ctgtcgcgcc tgattaaaca gcacggcgtg aagctcatcg ccatcggtaa cggcaccgcc
     8521 agccgcgaaa ctgacaaaat cgcgggcgaa ctggtgcgtg gaatgccgga aatgggactg
     8581 cacaaaatcg tcgtttccga agccggcgca tcgatttatt ccgcgtccga actggcggcg
     8641 cgcgagttcc ccgacttgga cgtttccctg cgcggcgcgg tgtccatcgc ccgcaggctg
     8701 caagacccgc tcgccgagtt ggtcaaaatc gaccccaaat ccatcggcgt gggccagtat
     8761 cagcacgacg tgaaccaaag ccggctcgcc aaatcgctgg acgcggtggt cgaagactgt
     8821 gtgaacgccg tcggcgtgga cgtaaacacc gcctccgccc cgctcttggc gcggatttcc
     8881 ggtttgaatc aaacccttgc ccaaaacatc gtcgcctacc gcgatgaaaa cggcgcgttc
     8941 gacagccgca aaaaattgct gaaagtaccg cgtttgggcg aaaaaacctt cgagcaggcg
     9001 gcaggctttt tgcggattaa cggcggcaca gagccgctgg acgtgagcgc ggtgcatccc
     9061 gaagcctatc ccgtcgtcgc caaaatgctg gcgcaacaag gcattaccgc cgccgaactc
     9121 atcggcaacc gcgagcgcgt gaagcaaatc aaagcgtccg acttcaccga cgaacgcttc
     9181 ggcctgccga ccattctgga catcctgtcc gagctggaaa aacccggccg cgacccgcgc
     9241 ggcgcgtttc agacggcatc gtttgccgaa ggcatccacg aaatcagcga cttgcaagtc
     9301 ggtatgattt tagagggcgt ggtttccaac gttgccaact tcggcgcgtt cgtggacatc
     9361 ggcgtgcatc aggacggctt ggtgcacatc tccgccctat ccaataaatt cgtccaagac
     9421 ccgcgcgaag tcgtgaaagc cggcgatgtg gtgaaagtga aggtgctgga agtcgatgct
     9481 gcacgtaaac gcatcgcgct gaccatgcgc ttggatgacg aaccgggcgg cgcaaaacat
     9541 aaaatgccgt ctgaaaaccg cagccgcgaa cggacagccg gccgcaaacc ccaacgcaac
     9601 gaccgcgccc cagccaattc ggcgatggcg gatgcgtttg cgaagctgaa gcggtagggg
     9661 ttgcctgaag cgtgattaaa aatatttgtt aaaacaatat attaaaatca acttgtaaaa
     9721 aaaatcctgt ttttttatca tatcggggcg atatagcaaa aaaacgggat ttttttatca
     9781 tttaaccgaa accttaaaat gtactcaatc gaacaattcc ctcccgaaat agattttgaa
     9841 acggtcgccg tcttgaaaaa actggcttcc gcccatcgtt atctggccga attaaaaggc
     9901 atctgccgca gtatccccaa ccaaggcatt ctgattaaca cgctgtcttt gcaggaagcc
     9961 aaagacagtt ccgaaatcga aaatatcatc accacccatg acgagctgtt ccgtgcagga
    10021 atatcggcaa gcccgtccag tcctgccatt aaagaagtgc agaattatgc ttcggcactg
    10081 cattgcggct tcggccttat ccgaaaacac ggaatactga ccaacaatca tattttggcg
    10141 atacaggccg agttggaaaa gaaccgcgcc ggattccgca aacagtcggg aacgatgctg
    10201 aaaaacgacc gcaccggaga aaccgtctat acgccgccgc aacatacgga cgacatcatc
    10261 cgcctgatgg gcaggctgga ggcatttgtc aatgacggca gcacggcaaa acccatcgat
    10321 ccgctgatcc gtatggcact gatccaccac cagtttgaaa gtatccaccc gttttacgac
    10381 ggcaacggca ggacaggcag gattatcaat gtgctgtatc tagttttaac cggattgctg
    10441 gatattcccg tcttatacct cagccgctat ctggtacgga ataaaagcga atattaccgc
    10501 ctgctgcaac acgtgcgcga tacgggcgaa tgggacggct ggctgttgta tatgctggaa
    10561 gccgtggaac aaaccgccaa ggacggcatc gataccgtgc agcacatcca tcaggcgatg
    10621 ttggactaca aacaccgcat caggaaaaac ttcagcttct acagccaaga cctgattaac
    10681 cacctgttca accatcccta cacaaaaatc gatttcctga tgaaaacgct gaacgtttcc
    10741 cgccccagtg ccgccaaata tctggacgaa ctaacagaag gcggattcct gcacaaagaa
    10801 aagataggca gaagcaacta ctatatcaat atcgcgctga tgaatatctt attgccgact
    10861 aattgataat atttgaaatt tatccaatat caaatcaata tgatgtttta cgccataccc
    10921 tccggactga cggtgcggac aggcgtattg tttcgatatg gtcaacgtta tataaagaag
    10981 gagtccgaac catgaaaaaa agttctgttt gccgcgctgg tgtcctgttt ttcggtttct
    11041 gcgtttgcgg cgtgtgccga cagccggcag cagtgcgtga tttataaaaa cggcaatgtc
    11101 gcggcggagg gcggctgcac ggtcagcaaa tgcgaggatg ccggggcgaa ggttttgaag
    11161 tggaagatga aaaacggcaa aggcgtaacc gttgaaatcg gcaaaaacgg caaggtttcg
    11221 gtgaacaaaa aaccgggtgc gaaggcggcc aacagtaatg cggcaggtat gggtttgacg
    11281 tgttatgccg ccgatgcgga caagcgcgag cagttctgtt cgaccaatta ttgaacaggt
    11341 gcgaatgccc gatgccgtct gaacggagtt tcagacggca ttttgtttgt aaaaaatagt
    11401 tgtatagtgg attaacaaaa accagtacgg cgttgcctcg cct
//
LOCUS       DAXIEB010000066        11257 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000066 DAXIEB010000000
VERSION     DAXIEB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.066"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(236..2644)
                     /locus_tag="ACFOA3_001381"
     CDS             complement(236..2644)
                     /locus_tag="ACFOA3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6898378.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPMQPVGLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADKVEGQSQ
                     VKVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIWGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATAEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2714..3718
                     /gene="amgK"
                     /locus_tag="ACFOA3_001382"
     CDS             2714..3718
                     /gene="amgK"
                     /locus_tag="ACFOA3_001382"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6898379.1"
                     /translation="MQRQIELKNWLQTVYPGRSFELAFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3775..4473)
                     /locus_tag="ACFOA3_001383"
     CDS             complement(3775..4473)
                     /locus_tag="ACFOA3_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6898380.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPTGL
                     VEGQNYTVLSNPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            4677..6215
                     /gene="murJ"
                     /locus_tag="ACFOA3_001384"
     CDS             4677..6215
                     /gene="murJ"
                     /locus_tag="ACFOA3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6898381.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATETFIRHVAGMLSFVLVIVTALG
                     ILAAPCVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFFLLRKHGIYRPGKGWAAFLAKMLL
                     ALAVMCGGLWAAQAYLPFEWVRVGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            6318..7274
                     /locus_tag="ACFOA3_001385"
     CDS             6318..7274
                     /locus_tag="ACFOA3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6898382.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRSRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGKKALPHAAA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTTFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7323..8105
                     /gene="trpC"
                     /locus_tag="ACFOA3_001386"
     CDS             7323..8105
                     /gene="trpC"
                     /locus_tag="ACFOA3_001386"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6898383.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            8171..10471
                     /gene="recQ"
                     /locus_tag="ACFOA3_001387"
     CDS             8171..10471
                     /gene="recQ"
                     /locus_tag="ACFOA3_001387"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6898384.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQEAIVNALAVGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQRLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(10613..11044)
                     /locus_tag="ACFOA3_001388"
     CDS             complement(10613..11044)
                     /locus_tag="ACFOA3_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898385.1"
                     /translation="MNMYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWG
                     DGYDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAME
                     LKAFPCLSSLFLTKWHKNLYDSGLTKTSTALPRLSSKRTIL"
     gene            complement(11076..>11257)
                     /locus_tag="ACFOA3_001389"
     CDS             complement(11076..>11257)
                     /locus_tag="ACFOA3_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898386.1"
                     /translation="KRPEFYYWIGMFYYGIISCWFDYQLISTKANSYKRKVKQYKIFS
                     VIFSVLIFISTIVKL"
BASE COUNT         2363 a         3135 c         3165 g         2594 t
ORIGIN      
        1 gctttggcag ctttggcagc tttggcagct ttggcagctt tggcggatgc ggtttttgcc
       61 ttttgcggtg cggcgtggac atcggctgct gccagcaatg cggcggcaat catcagggct
      121 ttgattttca tcattctttc cttatcgttt gaatcctgcc tttgatggcg ggtttggggt
      181 tgtcggtgga atgccgtctt gtgcgggaac ggtgctcctg ccgggtttcc gacggtcagg
      241 gtcgtttgtt gcgtccggcg gaaagagagt gggcggggat atagccggga acggcgacat
      301 ccatcctgtc tgcggggttt ctgccgacac tgctgaggtc tttcaactga agtgagaaaa
      361 agacagcgtt tttgtaggtg ttttcgccgg taacgtagcg ttgggcgtac acgcccgcgc
      421 cccagcagcc gcaactgctt ttgtattccg cacccgccag cacctctatc ggttttttgg
      481 cttcaaaacc gtagttgtaa cggacgacgg ccgacaggtt gcgcgtcagc ggccattgtg
      541 cggacaggtc gagctggctg agtttgtcgt aaaaatagga accgtcggac ttcaggtaga
      601 ttttttcgtt gcgcccgtat ttgtagcggg cgttcagcac tttgccctgt gcgggacggt
      661 agcttgcacc gacggcgtag ttctcggcgc gtttgtcgtt ttggttgtag tggatgctgc
      721 tgtcgaggat gaagcggctg ccgatgctgc cggaggcaaa tgccacccag tcggaacggt
      781 tgcgcggttt tttgccgacg ctgccgtcaa gcatcaccgc atcatccttg aaatagaatt
      841 tctgaccgat gccggcgcgg aaacgctctt ccgccgtcgc gccgtccaaa atacggcttt
      901 gcacggcggc ggaaaggctg tttgcggtgt taatcctgtc gttgccgtaa tagaggtttt
      961 cacgaaaaag ctgcccgtag ccgaagctgc tttccgacga atcgaaattg ggcaggtcgt
     1021 tttgggattt ggcaggaata tagttgtaga acaggcgcgg ctcgagggtt tgcaggactc
     1081 cgccgccgaa catccgcgta ttgcgttcga aggtcatgcc gctgtcgatg ttgacgatgg
     1141 gcagagtgcg gctgacgcgt cgggcttctt ggctgccgaa gcggttgagg ctgtaatagg
     1201 tggcgtgcag tccgagtttg ggacggacgt aaccccagct gttgctgaaa tcccatttga
     1261 tgtcgggata gacgacgagg cggctgccgt cttggcggct gtcgtggctg aagcgggtaa
     1321 attgggcgga cacgccgatt tgcgccctgc cggtgttttt gcgccaatcg gcggaaaggc
     1381 gcggcatcag ggcatacggt ttgtctttgt agccgctttg gtttgccagc gtctggtatt
     1441 tcagaaccga aaggccggca ttcaggctgc cgcccgccgc cctgccgcca taatccagcc
     1501 atacacggcg gttgaggttg acgttgccgg cgatttcttt gttgccgtaa aagtcacggt
     1561 agtagccgct gtcggagact tggttgaaat cgacacccgc ctgaagcgtg tcggaaatgt
     1621 cgtgccggtg ctgccatttc gcctgatagc ggttattcct gccgcttttt ttgtcgtgcg
     1681 gcagccaggt caggtcggac tggccggcat aatccggccg caggtagcgt acctgcccgt
     1741 caaagaccgc gccgcgttcg ccgatcacgc cgggcgcgaa cgtggcatcg agattggggg
     1801 caaggttgaa ataataggga acggaaaggg aaacgccgtc cgaaccggcg gacagtgagg
     1861 gaacaagcag gccgcttttg cggttgccgt caagcgggaa gtccgcccaa ggggtgtaga
     1921 aaatgggaac gccgccgaac acgaaggcgg cgtgtttggc aacgcctatg cctttttccc
     1981 gatcggcttc gacggaagcg gccttgacat accagccggc atcgccggcg gaacaggtgt
     2041 taaattgggt ttccgtcagt ttgtaatgcc cttcgcccaa catttcggcg gtgcggctga
     2101 cgctttgcag ccgccgtccg ccttgttcgg tttccatgcg gacgttgtgc gcttcgccgg
     2161 tctgctgctc gagattgtag gtcagggttt cgccccaaat cagcgtaccg tcctgttgga
     2221 gggcgaaccg gtcgcctacg gtaacggtgt cgcccgactg gtcgtaatcc gcccaatcgg
     2281 tattgaggac tgcgccgtcc cgttcgatga taacgcttcc ttccgcgcgt accttgacct
     2341 gcgactgccc ttccaccttg tcggcgacaa tgcgcgtata gtcttcgggg atggatgctt
     2401 cgccgctgcc ttggacggcg gcttcggttc tctcggggct gccgctttcg ttactgcaaa
     2461 acaggcaggt cgaaccgagg ctcaggccga caggctgcat gggttcggac acgctccgaa
     2521 cgcttcctcc ggcggtcgga ttgtccgttt cctccgccgc aacggcatcg gcggcggcgc
     2581 aatgcgtgcc gaagcagaag cccaatgcca gcaccagtgg tttgagtgaa aataaacgag
     2641 ccaaaattgc ccctcaggtc ggtttgccag ttagaatagc gtttattgta acctgaaatg
     2701 ctttagtact gttatgcaac gacaaatcga actgaaaaat tggcttcaga ccgtctatcc
     2761 cggccgcagt ttcgaactgg cctttgcggc ggcggatgct gatttccgcc gctatttccg
     2821 tgcaacgttt tcagacggca gcagtgtcgt ctgcatggat gcgccgcctg acaagatgag
     2881 tgtcgcacct tatttgaaag tgcagaaact gtttgacatg gtcaatgtgc cgcagatatt
     2941 gcacgcggac acggatttgg ggtttgtggt attgaacgac ttgggcaata cgacgttttt
     3001 aaccgcgatg cttcaggagc agggcgaagc ggcgcacaaa gccctgttgc tggaggccat
     3061 cggcgagctg gtcgaattgc agaaggcgag ccgtgaaggg gttttgcccg aatatgaccg
     3121 tgaaacgatg ctgcgcgaaa tcaacctgtt cccggaatgg tttgtcgcaa aagaattggg
     3181 gcgcgaatta acattcaaac aacgccaact ttggcagcaa accgtcgata cgctgctgcc
     3241 gcccctgttg gcgcagccca aagtttatgt acaccgcgac tttatcgtcc gcaacctgat
     3301 gctgacgcgc ggcaggccgg gcgttttaga cttccaagac gcgctttacg gcccgatttc
     3361 ctacgatttg gtgtcgctgt tgcgcgatgc ctttatcgaa tgggaagaag aatttgtctt
     3421 ggacttggtt atccgctact gggaaaaggc gcgggcggcc ggcctgcccg tccccgaagc
     3481 ttttgacgag ttttaccgct ggttcgaatg gatgggcgtg cagcggcact tgaaggttgc
     3541 aggcatcttc gcacgcctgt actaccgcga cggcaaagac aaataccgtc cggaaatccc
     3601 gcgtttctta aactatctgc gccgcgtatc gcgccgttat gccgaactcg ccccgctcta
     3661 cgcgctcttg gtcgaactgg tcggcgatga agagctggaa acgggcttta cgttttaaac
     3721 ccaatcaaaa tgccgtctga aaaccaagtt tcagacggca tttttcaaac gggcttactg
     3781 cgcggctttt tgttcttcgc gtactttgtc cgccaaaagg tcgatggtgt tcataccgga
     3841 ttcccagtcg gcaaattcga ctttatattt gccgccgacg ataaccgtgg gcgtaccgtc
     3901 gatttgaaag gtttcggtca gctcctgcat tttgtcggcg cgcgcctggc tttcgggaga
     3961 ttcgtaagcg gcaaggactt ttttgccgtc aaaggcggtt tgttcgccca accatttttt
     4021 gaggacttcc ggattttgca gcttgatttt ttggttgacc atcgcatcga aaatatggct
     4081 gttcgccaca tctttgctgt cggcggcagc catatcgacg gcggcggcga ggcgtgccag
     4141 cgtcagcatt tctttctgcc agacgacgtg ttcggtacgc aggtacatat cgtctttaaa
     4201 agacttggcg tgtttgctca aaacaggttc gaggtgggcg cagtgcgggc agaaatagcc
     4261 gaaaaactca aggacttcaa ccttgcctgc ctgctgttgg ggaatcgggt tggaaaggac
     4321 ggtatagttt tgcccttcga ccagccctgt cggggcggcg gctgccgaag cggcaggcgc
     4381 gctgtcggcg gggacgctgg tttggacttt gctgtcgcac gcggcaaggg cgaacagggc
     4441 ggcaacgccg agggcaaggt gtctggattt catacagctc tccgtgatgt tggaaataaa
     4501 tcggatattg gtattttatt gtattttcgc gtgttgatac agtttgcctc tggaaaagga
     4561 agctttcgta tcgggaaacc ggttcggacg gcatgaaacc ctatgccgtc tgaaccggtt
     4621 tctgtcgtac aatacgcgcc gttgccccaa acgggtacga ctgttgagga acaatgatga
     4681 atatgcttgg agctttggca aaagtcggca gcctgacgat ggtgtcgcgc gttttgggat
     4741 ttgtgcgcga tacggtcatt gcgcgggcat tcggcgcggg tatggcgacg gatgcgtttt
     4801 ttgtcgcgtt caaactgccc aacctgcttc gccgcgtgtt tgcggagggg gcgtttgccc
     4861 aagcgtttgt gccgattttg gcggaatata aggaaacgcg ttctaaagag gcgacggaga
     4921 cttttatccg ccacgttgcg gggatgctgt cgtttgtgct ggtcatcgtt accgcgctgg
     4981 gcatactcgc cgcgccttgt gtgatttatg tttccgcacc cggttttgcc aaagatgccg
     5041 acaaatttca gctctccatc gatttgctgc ggattacgtt tccctatatc ttattgattt
     5101 cactttcctc ttttgtcggc tcggtactca attcctatca taaattcagc attccggcgt
     5161 ttacgcccac gttcctgaac gtgtcgttta tcgtattcgc gctgtttttc gtgccgtatt
     5221 tcgatccgcc cgttaccgca ctggcatggg cggtttttgt cggcggcatt ttgcaactcg
     5281 gctttcagct cccttggttg gcgaaactag gctttttgaa actgcccaaa ctgaatttca
     5341 aagatgcggc ggtcaaccgc gtgatgaaac agatggcgcc tgcgattttg ggcgtgagcg
     5401 tggcgcaggt ttctttggta atcaacacga ttttcgcgtc ttatctgcaa tcgggcagcg
     5461 tttcatggat gtattacgcc gaccgcatga tggagctgcc cagcggcgta ctgggggctg
     5521 cactcggtac gattttgctg ccgactttgt ccaaacactc ggcaaaccaa gatacggaac
     5581 agttttccgc cctgctcgac tggggtttgc gcctgtgcat gctgctgacg ctgccggcgg
     5641 cggtcggact ggcggtgttg tcgttcccgc tggtggcaac cttgtttatg taccgagaat
     5701 tcacgctgtt tgacgcgcag atgacgcaac acgcgctgat tgcctattct ttcggtttaa
     5761 tcggtttaat catgattaaa gtgttggcac ccggcttcta tgcgcggcaa aacatcaaaa
     5821 cgcccgtcaa aatcgccatc ttcacgctca tctgcacaca gttgatgaac cttgccttta
     5881 tcggcccact gaaacacgtc ggactttcgc ttgccatcgg cttgggcgcg tgtattaatg
     5941 ccggattgtt gttcttcctg ttacgcaaac acggtattta ccggcccggc aagggttggg
     6001 cggcattttt ggcgaaaatg ctgctcgcac tcgccgtaat gtgcggcggc ctgtgggcgg
     6061 cgcaggctta cctgccgttt gaatgggtac gcgtcggcgg aatgcggaaa gcggggcagc
     6121 tctgcatcct gattgccgtc ggcggcggac tgtatttcgc ctctctggcg gctttgggct
     6181 tccgtccgcg ccatttcaaa cgcgtggaaa gctgaccgga gcgccgcctt ttgccggcaa
     6241 acgctttttc cgcgcgtccg tgccggacgg gctgccgcat catcaatgcc atctgaaaac
     6301 agaaaaaccg ataccttatg attttgacac cgccggacac accgtttttc ctccgcaacg
     6361 gcaatgccga tacgattgcc gccaaattcc tgcaacgccc cgcacccgca taccgccgcg
     6421 agctgcttcc cgacagcacg ggtaaaacca aagtcgccta cgacttttca gacggcattt
     6481 cgcccgatgc gccgctggtc gtgctgtttc acggtttgga gggcggcagt ggcagccatt
     6541 acgcggtcga actgatgctc gcggtacgca gccgcggttg gaacggcgta gtcgtccatt
     6601 tccgcagctg cggcggcgta gcgaacaccg ccccggtgtt ctaccacttg ggcgataccg
     6661 ccgaaatcgc ctttactttg gacacgctcg ccgcgcgtta ccgtgaaata tacgccgtcg
     6721 gcgtatcgct gggcggcaac gcgctggcaa aatatttggg cgaacagggc aaaaaggcat
     6781 tgccgcacgc cgctgccgcc gtatccgccc ccgtcgatgc agaggcggca ggcagccgct
     6841 tcgacagcgg catcacgcgg ctgctctaca cgcgctactt cctccgcacc ctgataccca
     6901 aagcacgttc gctccaaggc tttcagacga catttgccgc agggtgcaaa acactgggtg
     6961 agtttgacga ccgtttcacc gcaccgctgc acggctttgc cgaccggcac gactactacc
     7021 gccaaacttc ctgcaaaccg ctgctcaaac acgttgccaa accgctgctc ctgctcaatg
     7081 ccgccaacga ccccttcctg ccgcccgaag cgctgccgac accggacgaa gtgtccgaag
     7141 ccgttaccct gttccagccg gcacacggtg gtcatgtcgg ctttgtcagc agcaccggcg
     7201 gcaggctgca cctgcaatgg ttgccgcaga ccgtcctgtc ctatttcgac agcttccgta
     7261 caaacaggcg ttaacggttt gatgctaata ttcccctttc ccagacgaat acggaacccg
     7321 acatgaccga catcctcaat aaaatccttg ccaccaaagc acaggaagtt gccgcccaaa
     7381 aagccgccgt caacgccgaa cacatccgcg cacttgccgc agaagccgcg cccgtccgca
     7441 gcttcatcga ttcgatacgc ggcaaacacc gcctaaacct gcccgccgtc attgccgaaa
     7501 tcaaaaaggc aagcccgagc aaaggcctga tccgtccgga cttccgaccg cccgagattg
     7561 cccgcgccta tgaaaacgcc ggggcggcgt gtttgtccgt actgaccgac gaaccctatt
     7621 tccaaggttc gcccgaatac ctcaaacagg cgcgcgaagc cgtatcgctg cccgtgctgc
     7681 gcaaagactt catcatcgac gaatatcagg tttatcaggc gcgcgcatgg ggggcggatg
     7741 ccgtcctgct gattgccgca gcactggaac aggaacaatt ggaacgcttt gaagcggtgg
     7801 cgcacgaatt gggcatgacc gtcctgctcg agctgcacga cgaaaccgaa ttggaaaaat
     7861 gccgcaacct gaccacgccg ttgtggggcg taaacaaccg caacctgagg acttttgaag
     7921 tctccctcga ccaaaccctg tcgctgctgc ccgcactgga aggcaaaacc gtcgttaccg
     7981 aaagcggcat tacgggcaag gcggatgtgg aatttatgca atcgcgcggc gtgcacacct
     8041 tcctgattgg cgaaacgttc atgcgtgccg acgatattga ggcggaagtg ggcaaactct
     8101 tctaaatctc gatttcagac ggcatattgc cgccgaccaa tcgacaccca ttaccggaca
     8161 cggaaccatc atgcaccgac ccaccgccaa acagattctg cacgaagtat tcggttatcc
     8221 cgaatttcgc ggcaagcagg aggctatcgt caatgctttg gcagtcggcg agagtttgat
     8281 ggtgctgatg ccgacgggcg ggggcaagtc tttgtgttac cagattccgg cgctgatgcg
     8341 cgaaggcgtg gcggttgtcg tatcgccgct gattgcgctg atgaacgacc aagtggccag
     8401 cctgcatgtg gccggcatcg aagcggcggc agtcaacagc ggcacatcgg cagatggggc
     8461 gcgcgagatt gccgacaagc ttgcccaagg ccgtctgaaa ctgctttatg tcgcaccgga
     8521 acgcctagtt accgaccgct ttttgcgttt tctcgaccaa caaaccgtca gcctgttcgc
     8581 cattgacgag gcgcactgcg tcagccagtg gggacacgat ttccgccccg aatatcaacg
     8641 gctcggtata cttgccgaac gctatccgaa cgttccgcgc atcgccctga ccgccaccgc
     8701 cgatgccgcc acgcgcgccg acatcaagca ttatctgcac ttggacgatg cgcccgaatt
     8761 tgtctccagc tttgaccgcc cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa
     8821 aaaacaattg ctggatttca tccgcaaaga aatgacgggg caaagcggca ttgtgtattg
     8881 cctaagccgc aaaaaggttg aagatgtggc gcggtttttg cgtgaaaacg gattaaacgc
     8941 gattccgtat catgccggtc tgagtatgga cgtgcgcgag gaaaaccaac gccgctttac
     9001 gcatgaagac aatattatcg tggtggcgac cgtggcgttc ggcatgggca tagacaaacc
     9061 cgacgtgcgc tttgtcgccc atctcgatat gccccagagt gtcgaacatt tctatcagga
     9121 gtcggggcgc gccggtcggg acgggctgcc tgccgcgagc tggctgtgtt acggtttgaa
     9181 cgattgggtg ttgctgcgcg aacggattgc cgaaggcaac agcgacgagg tgcaaaagca
     9241 aatcgaaatg caaaaactcg atgccatgct tgccgtctgc gaaaccgccg cctgccgccg
     9301 cgtactgctg ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt gcgacaactg
     9361 cctgcatccg cccgtacggt ttgacggcac ggtgttggtg caaaaattac tcagctgcgt
     9421 gtaccgcgcc ggacaacgtt ttgccgccgg ttacatcacc aaccttttgc gaggtaaaag
     9481 cgacgattgg atacgcggca accggcacga acaactgtcc acattcggca tcggtacgga
     9541 gttgtccgac aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg gctacctcac
     9601 cgtcaacatt acccgatatc aggcattgca actgaccgaa gccgccaaaa aagtcctcaa
     9661 aggcgaaacc gaagtgatgc tgcgtccgct caagcgcgac aagcccgccg cccgcaccct
     9721 caaagacaac tggctgcgta ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg
     9781 gcgtatgaaa caggcagaag ccgaaggcat ccccgcctat atgattttcg gcgacaaaac
     9841 cctgcgcgac cttgtcgaaa aaatgccgca aaacctcaac gggctgcacg acatctacgg
     9901 cttgggcgaa gccaaaaccg aacgtttcgg acacggcata ctcgaagtct gccgcaacgc
     9961 cgccggcttc agccgcgacg cggtcatccg tccgcaaacc gaacgcgaac aacaactgcg
    10021 tcaaaaactc gaggcctggc ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc
    10081 cgtcctctcc gacgaaagcc ttgccgatat gcttgccgat acgcccgaaa ccgaaaccga
    10141 cctcgaaggc gtacacggct tgggcagcgt acgcgccgcc aaatacggac gggacatcct
    10201 cgccgtctgc cgtccgtttt cagacggcat cgatgaaacc gccaaacaca aacgccgcct
    10261 gatgcgcgcc ctgatccaat ggtgcaacga aacggcaaaa cacgaacagt ccgaacccta
    10321 ccgcattctc agcaaagccg ccctacgcgc cattgccgcc aaacagccgg aaggtttggc
    10381 ggagcttgcc gccgtatacg gcgtaggcga agaaaaagcc gcacgttacg gtgcggcggt
    10441 gttggcggta ttggaacgaa atgccgtctg aagcccgtta ccaagtttca gacggcattg
    10501 cctctattta aaaattcctg tttttatagt ggattaacaa aaatcaggac aaggcggcga
    10561 gccgcagaca gtacggatag tacggaaccg attcacttgg tgcttcagca ccttagagaa
    10621 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactatcat
    10681 atagattttt atgccatttg gtcagaaaca gcgaagacag gcagggaaac gccttcagtt
    10741 ccatcgcgtc ttcaaaatca tcccaaacat cgctcaaatt ctgtttggat atgccgtatt
    10801 cccgtccggc aaacatcacg gtctttttgc ttttagcggc ttttttgaat gcctttctct
    10861 gtttttcgga tatctgctgc catctcaact gacggtacac gtcgtagccg tcgccccaaa
    10921 aagaggcata ccgttgcgtg tcttcgccga ccagcggcgc ggtattccca acccgtacat
    10981 atcccgtcgg cacgacggca cttgccttat acatgtgcag accgcgttcc caatacatat
    11041 tcaattgaat cgctcataaa tttttgctcc aaaatttaaa gttttactat agtagaaata
    11101 aatatcaaaa ctgaaaatat tactgaaaaa attttatatt gtttaacttt tcttttatac
    11161 gaattcgcct ttgttgaaat taattgataa tcaaaccagc aagaaataat tccataataa
    11221 aacattccaa tccaataata aaattcaggc ctcttag
//
LOCUS       DAXIEB010000067        11248 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000067 DAXIEB010000000
VERSION     DAXIEB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.067"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(229..1665)
                     /locus_tag="ACFOA3_001390"
     CDS             complement(229..1665)
                     /locus_tag="ACFOA3_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6898387.1"
                     /translation="MFIKSICLSNFKGFIGDNHQIKFKIPDGATAGSGLNIFIGENNS
                     GKSSIFEAINFLRNGIKEDEAYRIKSKLADDTQPNDACVELAFCGNIQNAITYFVQRN
                     KQAAFNNAINNEQNLKAKRNTVSCKKLDLWDNESQDYSNPSGIDAPFKALFETNFIWA
                     DTNPNDEAAFGSTTLCGLLLKEIAQAHINSAEYQTFRDSFNQVFNSPASNLRQQIATI
                     EQKVQRIFTEQFGQADIHFRFDELKIDSFFKNASIFIDDGVDIPMSEKGNGMQRSVAL
                     ALLQVYAEELAHDEEQGQTKPFYLFIDEPELCLHPKGQTKLLEALLEISRTKQVFLTT
                     HSPYFLVTPHLSNVGLFIFRKDGISNIVEDASLEPMFPWSPTWGEINFKAYKLPTVEL
                     HNELYGKLQHDCGQFREKDFEQWLQSRNIQFTKQWIRENNGAAQAPYDVTLQTFIRNH
                     IHHPENRRNALYTENELRQSIEEMIRLL"
     gene            complement(1814..3193)
                     /gene="norM"
                     /locus_tag="ACFOA3_001391"
     CDS             complement(1814..3193)
                     /gene="norM"
                     /locus_tag="ACFOA3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6898388.1"
                     /translation="MLLDLNRFSFPVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            3528..4679
                     /locus_tag="ACFOA3_001392"
     CDS             3528..4679
                     /locus_tag="ACFOA3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6898389.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            4782..6080
                     /locus_tag="ACFOA3_001393"
     CDS             4782..6080
                     /locus_tag="ACFOA3_001393"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6898390.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            6545..6928
                     /locus_tag="ACFOA3_001394"
     CDS             6545..6928
                     /locus_tag="ACFOA3_001394"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6898391.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            6942..7352
                     /locus_tag="ACFOA3_001395"
     CDS             6942..7352
                     /locus_tag="ACFOA3_001395"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6898392.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            7469..7861
                     /locus_tag="ACFOA3_001396"
     CDS             7469..7861
                     /locus_tag="ACFOA3_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6898393.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            8081..8461
                     /locus_tag="ACFOA3_001397"
     CDS             8081..8461
                     /locus_tag="ACFOA3_001397"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6898394.1"
                     /translation="MTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQLLYY
                     DTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYG
                     SIRTKLDGRSNPDNLVCNRYKVLL"
     gene            8575..9135
                     /locus_tag="ACFOA3_001398"
     CDS             8575..9135
                     /locus_tag="ACFOA3_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898395.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(9436..10275)
                     /gene="htpX"
                     /locus_tag="ACFOA3_001399"
     CDS             complement(9436..10275)
                     /gene="htpX"
                     /locus_tag="ACFOA3_001399"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6898396.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            10490..11137
                     /gene="adk"
                     /locus_tag="ACFOA3_001400"
     CDS             10490..11137
                     /gene="adk"
                     /locus_tag="ACFOA3_001400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6898397.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
BASE COUNT         2997 a         2725 c         2762 g         2764 t
ORIGIN      
        1 gacagctcca tatgttttta tcaaaacaaa gattaaacag gtttattgct gtttatcctc
       61 aaattgtcct tatttcagca ggcgatcaaa gttttttagt ttttaccatc atcaacggtt
      121 acccaaaaat tttgaataac tgaattttct tctttgggct agaaagtgtg gcttcagacg
      181 acgttttgaa aacattgaga gaattgaccg cactttttac tccttcaact ataacaatct
      241 aatcatttcc tcaatagatt gtctaagttc attttccgta taaagtgcat ttcgccgatt
      301 ttcaggatga tgaatatggt tgcggataaa tgtttgcaag gttacgtcat atggagcttg
      361 tgcggctcca ttgttttctc gtatccattg tttagtgaac tgaatattcc tactctgtaa
      421 ccattgttca aagtcttttt ctcgaaattg cccacaatca tgttgtaatt tcccatataa
      481 ttcattatgt aactccacgg taggtaattt atacgccttg aaattaatct ctccccaagt
      541 tgggctccaa ggaaacattg gctccaatga agcgtcttca acaatattgc taattccgtc
      601 ctttctaaaa ataaacagac caacattact taaatgaggt gtaaccaaaa aatatgggga
      661 atgcgtagtc aaaaatactt gtttcgttct tgaaatttct aacaacgcct caagtaattt
      721 agtttgccct tttggatgta aacaaagctc aggctcatca ataaacaaat aaaacggctt
      781 agtctgacct tgctcttcat catgcgctaa ctcttctgca tagacttgaa gcaaggctaa
      841 tgcaacagaa cgctgcatac cattaccttt ttcggacatt ggaatatcca caccatcgtc
      901 aataaagata gaggcatttt taaaaaacga atctatcttt agttcatcaa atctaaaatg
      961 aatatcagct tgtccgaatt gttcagtaaa aatacgttga actttctgtt cgatagtagc
     1021 aatttgttga cgcaaattag aagctggtga attaaatacc tgattaaaac tatcacgaaa
     1081 tgtttgatat tcagccgaat tgatatgcgc ctgagcaatt tctttcagta acaatccgca
     1141 aagagttgta gacccgaaag ccgcttcatc attaggattt gtatctgccc aaataaaatt
     1201 agtttcaaaa agagccttaa aaggagcatc gatcccactt ggatttgaat agtcctgaga
     1261 ttcgttatcc cacaaatcca atttcttaca agaaactgta tttctcttag cttttaaatt
     1321 ctgctcatta ttaattgcat tattaaaagc tgcttgttta ttcctttgaa caaaataagt
     1381 tattgcattt tgaatattcc cacaaaaagc cagctcaacg catgcgtcat ttggctgagt
     1441 atcatctgcc agtttacttt ttattctata agcttcatct tctttgatac cgttccttaa
     1501 gaaatttata gcttcaaaaa tagacgattt cccagagtta ttttctccga taaaaatgtt
     1561 caaaccacta ccagcagttg ctccatcagg aattttaaat ttaatctgat ggttatcacc
     1621 gataaaccct ttaaaattag ataaacaaat actttttata aacataacgg tttccttatc
     1681 ttaagttaga aatagagatt aagctaaaac accaaactct ctcccttcaa aaactctaaa
     1741 ttctcctagc tcaaccctgc cacatcatct attaatattg taattagcag acttcatcct
     1801 acccatggag ttttcagaca gccttatgcg atctgaccat ctccctactg cacaattcca
     1861 agcaccacac caaggcgatg gcggcgatgg tgagcgaggc aatcaatgcc gtccagaagc
     1921 cgtaaatgcc catatcgaaa cggtaggcga gcaggtagcc cggcagcaga ccgcagcccc
     1981 aaaaggcggc ggcgtggatg aacatcggca cctttgtaac tttgtagccg cgcaaggcgt
     2041 aggaggcgat acattgggtg aagtctgccg gttggaacaa gccggcgaac agtaagacgg
     2101 tggcggcgat gcttaaaacc gccggatcat tgttgtacat acttaccagc ggcgaacgga
     2161 ataataccaa ggaaagcacg gtaatcacgg cgagcatcca tcctaacacc agtgacacgc
     2221 ccgaaatata acgcgcccgc gaaaattcgc gccgcccaag cgaaaagccg atgcgcaccg
     2281 tccccgccga gccgacgctt tgcggaatca tatagagaat ccccgacaaa ctgatgccga
     2341 cctgctgcgc cgccacataa tcctcgccga aaggcgcaat caaaaacacg ataaacgaaa
     2401 acgcgctggc ttccaaaaaa taagacagcc cgatgggtgc gccgattttc caaatctgtt
     2461 tgaacaccgc ccaatccggt ttgccgaatt tcgccgtcag tccgaatggg cggaagaaat
     2521 tttccttggc gatataaatc cacaatgcca gcgcgctgaa ccaaaacacc gccatcgtcg
     2581 ccagtccgca gcctgcgccg cccaaagcgg gcataccgaa tttgccgtaa acgaaaatat
     2641 agttcagcgg cacgttcaac acaaacgccg caaagctgac caacataatc aggcgcgggc
     2701 ggttcaggct ggaggcgtag gcgtgcagtg cgcggtgtac cattgccgcc ggcatcgcca
     2761 agctggtgaa cagcatatac tgcgccattg tgccttccac ataatcgctc aaggtcagcc
     2821 agttgcggaa cggcgtaatc gccgcccaca tcaagaccat gccgaacacg cccaaaaaca
     2881 gcccgaacca aatcccctgc cgtcccgttt cgcccacttc gtcggtttta cccgcgccgt
     2941 aaagctgggc aatcatcggg ttcagcgccg ccataatgcc cataaaggta atataaaccg
     3001 tggcaaacgc gctgctgccc aaagccaccg ccgccaaatc ttccttaccc gcaccgcccg
     3061 ccatcacggt atcgacaaaa ccgatgccca cctgcgcaac ctgtgccaac aacatgggca
     3121 gggcaagggc ggtcagcagg cggatttctt tcaggaagac gggaaaggaa aagcggttga
     3181 ggtcgagcag cataagtgtt caatcaacaa aaatgccgtc tgaagcagaa aacggcagca
     3241 ggaagtgatg agaaataatg gtgcacatta tatagtaaaa aaatgccgtg ccgtcagacg
     3301 gcggatacag ggtataaaag tatattcaga ttgtgtgtat tttatggtaa agtttggttt
     3361 taacgacttg acggcattga gccgtcggac aggggctgtt cggattctga atcagaaaga
     3421 agcatcgccg ttttgacagc ggcgtgatgc gttgcggcaa agatgccgtc tgattgcgga
     3481 tcgggcagtc ttttgtgttt acaggataaa atagaaggca gattctcatg cagacatggc
     3541 agcttccgga acatatcgcc gacgtactgc ccacgaacgc gcggcagctt gaaagcgcga
     3601 gggagcagtt gttggcactg ttccgtgtac acggttatga actggtacag cctccgctga
     3661 tggagtacgc acattccctg ctgacgcata tcgatgcggg gctttccctg aaaaccattt
     3721 tggtaacgga caggctcagc ggcaggcagt tgggcatacg cgccgacatc acgccgcagg
     3781 tggcgcgtat cgatgcccat cttttatccg ccaaccaagg gattaaccgg ttgtgttatg
     3841 ccggtccggt gttgcacgcg cagtccgacg gtctgctgaa tatgcgcgaa cccttgcagg
     3901 caggggcgga aatgtacggt tttgccgaca tccgtggcga catcgagctg atagacctga
     3961 tgctgaaaag catgaaaatt gccgatatgg gcaaagtgct gctttcgctg gggcatatcg
     4021 gcatatttcg cgccttgtcc gatgcggcgc atttggatgc ggggcagtct gcaacgctgc
     4081 ttgccttgat gcaggataaa gataccgaga cggtcgaagc gcaggtcaag gcttggaagc
     4141 tggacggtat gtgggcaaaa gcattctcgc tgctgccgcg cctgtacggc gggcgtgaag
     4201 tgttgtccga cgcgcgcgga cggttgccgg atttgtcggc ggtcggcggc gcgttgggcg
     4261 aattgcaggc ggtgtgcgac gcattccccg attgtgaaat ccatatcgac ttgtccgagc
     4321 tgcgcgtcga caattaccac acgggcttgc tgtatgccgc ctatgccgcc gatttccacg
     4381 acgcggtcgc gcgcggcggg cgttatgacg gattgggcgg atatttcggc agggcgcgcc
     4441 cggcaacggg attcagtttc gacttgcgca gctttatcgg gcgtttgccc gccatcgaac
     4501 ggcagcccgc cgtgttggtc gatgcggaag atgccgaagc ggcgcacgaa gcggtcgaag
     4561 ccttgcgtga acaagggcag tgtgtcgtaa tcgattacgg tatcggacac aatgtttcgg
     4621 aagagcttgc aggccgtctg aaaaagacgg acggcgtttg gcaggtcgtg aaacgctaaa
     4681 tacccgttca tggcggatga aaggcaaatc gtggcggggc gcaaagccgc accggtttgg
     4741 ggatttccgc aataattttt aatatcgaag gttatatggc tatggctaaa aatgttgtag
     4801 taatcggcgc acagtggggc gacgagggta aaggtaaaat cgttgactgg ctggcggaag
     4861 aagccggagg cgtggtgcgc ttccaaggcg gccacaatgc gggtcatacc ttggttgtcg
     4921 gcggcaaaaa aaccattttg cgcctgattc cgagcggcat cctgcatgaa agtctggact
     4981 gcttcatcgg ttcgggcgtt gtcgtctccc ccgaagccct gttgggcgaa atcgacgagt
     5041 tgaacgcggc aggcgtgaaa aacgtcgaag gccgtctgaa aatcgcgccg acctgcccgc
     5101 tgatcctgcc ttatcacatc gcgctcgacc aagcccgcga agcatcgcgc ggcaaaggca
     5161 aaatcggcac gaccggccgc ggcatcggcc ctgcctacga agacaaagtg gcacgccgcg
     5221 ccattcgcgc cgccgatttg ctgcatcctg aaaaactgcg tgaaaaactg gatgtcgtcc
     5281 ttgcttatta caacgtccaa ctgcaacatc tgcacaatgc cgaaccggtt aaagcggaag
     5341 acgtgatggc ggttatcgaa aaagtcgcgc cgcgcattgc gccgatgatt accgacgtgt
     5401 cccgcgtgtt gaacgagaaa aacaaaaacg gcgaaaaact gctgtttgaa ggcgcgcaag
     5461 gtgcgttgtt ggacatcgac tacggcactt atcccttcgt tacctcgtcc aactgcctcg
     5521 ccggcgcggc ttcggcaggc gcgggcgtag gccctcaaat gctggattat gttttgggca
     5581 tcgtcaaagc ctataccacg cgcgtcggtt cgggcccgtt cccgaccgaa ttgttcgacg
     5641 aagtaggcgc aggtttggcg gagcgcggac acgaattcgg ttctgtaacc ggtcgtgcgc
     5701 gccgctgcgg ctggtttgat gccgccgccc tgaaacgctc catccaaatc aacggcattt
     5761 ccggtatgtg cattaccaaa ctcgatgtaa tggacggcgt tgaaaccatc aatatctgcg
     5821 tcggctacga attgcccgac ggcggcaaaa ccgacatcct gccttgcggc tctgatgcgg
     5881 tagaagcctg caagccgatt tacgaaacca tgcccggctg gcgtgaatcc actttcggcg
     5941 tgaaggacta cggcgcattg cccgaaaacg ccaaagcata tttgaaacgg attgaagaag
     6001 tctgcggcgc gccggtcgcc atcgtctcca ccggccccga ccgagaagaa acgattgtgc
     6061 tgcatcatcc gttcgcataa ggttttgcag taaaattgcc gtctgaagcc ctaatccgcc
     6121 gttggtcata aatagtaggg tatcaacatt tcgggctaca atggtacgtc agccaatgcc
     6181 aagacgtgcc agcctgattt gttgatgtgt gtactattgc ataggcggtt aagccatgca
     6241 ggagatgaaa gtgtatattt cgcgcaaagc ccattagccg caagcgaggg gcggggtgcg
     6301 cgagagagag gggggcaggt gctaagtcat tggttatctc cttactatct atctaactac
     6361 gggtgcatta tgcgcctatg cctgttgttt gtcaaacact atacagttaa aatgtgtaaa
     6421 gatttagtaa ggacatatac ccttttcttt acatttagct ccatcggata ccagtgcgtt
     6481 cagagaaaat ttgaaaattt ctatattttg gttgtataat gctttcattt tagaaaggtc
     6541 tataatgcca aaatcactct cgctacaaga tgttcagtta aggttttcca gtaaatttcc
     6601 tgataagacg gttttgaagt tcactaaaaa ctacgaaccg gtaacaatcc aatgcccttt
     6661 gcatgggtcg gttgtttacg ggaatctaca agctgccatg aaatcctcaa caggatgtcc
     6721 tgaatgtact cgaaatcccg aaaagccctc tccaaacgcc gttgccatta ggttggagga
     6781 cacagagaca ggggaaatct acgagtttgc aagtacactg gctgccagta aatttatcgg
     6841 ttgctccaac agcactttag ctgtacgttt aagcggacgc actccgtttg accgattgat
     6901 taggtatcgt tataggttgg taaagtaagt tcacaaccac tatggcactt acatttactc
     6961 aagcagtttc taagctaacc tctaaatttc cacatttgaa tcttgtggag ttcaatggcg
     7021 ttcgttaccc gacggtaatc gtctgtccta tacacgggag ggttacttgc tctacattca
     7081 aaagtatgtt ggactctaaa agtgggtgtc caaaatgtgc atcttatggt gtcaattccc
     7141 acaaaattcc agaagataca atagataaat tatctaaaaa tacagtattg gaggatactg
     7201 taactggcga aacacttaca ttcccctcaa gagcatctgc tgcaaggtca ttgggtataa
     7261 acccagcagc tatcactgac cgtataaaag gtcgggttca cacagagact ttacttgcag
     7321 ggaggtataa ggttcacatc tgcactaaat gacgtataca ctttttaaca gtgtataccc
     7381 ctcgccacta tcaggtgctt ctcgaaaaat ttgacatttt tataaatttg ctattaaatc
     7441 catttgacaa tcaattttgg agtctcaaat ggccaaatct ttcaaccaag cagcttctga
     7501 acttactgat atattcccta atatctctct aaccggcttt gacggtgtga attacccagt
     7561 aaccgttaat tgtcctatgc acgggaacgt tcgttattcg acatttaatg cccttataaa
     7621 gtcaaagtac gggtgtcctg agtgtgctaa gatgtcaaaa acccaaacac ctccaaatgt
     7681 agggaagccc ctcctcatcc tcgacacaac gaccaacgaa acactcacgt tcccaagcgt
     7741 tactgccgca ggtgctgctt taggtgtaca tttccaacaa atcaatcaca ggctcaaggg
     7801 tcgaacgtcg cccgacaacc ttatttcaaa caggtacaaa gtgcttgggt acgataggta
     7861 aggttcacaa caccctaaat cctacgaacc aagctcgacg gacgttcaaa tcccggttag
     7921 agttaattta tgtaaagatt tagtaaagac gtataccatt tttctttaca ttgtgcttgc
     7981 gcaggatttc aggtaggtct caaaaaattt gaaaatttct atattttggt tgtgtaatcc
     8041 atttgcacat aacctatgga gacaaattat gggtaagcga atgactttcg ataccgccaa
     8101 atcacgcttt caagagaaat ttccacattt agaattgttg gagttcagtg gcatttataa
     8161 accttccagt gttagatgtc ctacgcacgg ggttgtccaa cttttgtatt acgacacagc
     8221 tataaagtca aagtatgggt gtccggagtg cgggaaactt aaaatgaagg aaaatacgcc
     8281 tccccaaaac caaaaacctg tctccatcct cgacaccgcc acaggcgaaa cactcacgtt
     8341 ccccagcgta caagctgccg ccaaagccct aaacaccccc tacggctcta tacgaaccaa
     8401 gctcgacgga cgttcaaatc ccgacaacct tgtctgtaac aggtataagg ttctgctata
     8461 atcaacctat ggaaaatatt gaagaatacg cactgctgtc tcccgaagcc ctgctggaac
     8521 gcctggatac cgttttgagt atcagaatcg gcggcaaggg ttgggaatcc agttatgacc
     8581 gccaactttg cacagacgct ggtcgaaata caggacagtc tgtacagggt tgtgtcaacc
     8641 gtccaatacg gggatgacaa cctcaagcgg ttgacagcgg acaaacggaa gcagtatgag
     8701 ttgaacttca agatttccga gggttctacg cgtgtagagt ccgactttaa agagactttg
     8761 gttcggttcg gtagagatat gcttcaagat atgcccccta aaatccgttc ggcaacgctg
     8821 gtagcgttga cgaccctgct tgtcggaggg gcgttgggtt acggttattt ggaatacctg
     8881 aagcaggttg cttcggaagg gtatcagacc gagcgtctgt ataatgccgt cgaccgtctt
     8941 gcagaatccc aagaacggat aacgtccgcc atcctgaagg gtgctagagg tgccgatttc
     9001 gtgcaaatcg gcagacgttc ctacagtagg gaggatatat cggaggcaaa tagacgtgca
     9061 gagcgtgtcc cgtatggcgc agagttggtt tcagacggca attttaccgc tgttttatct
     9121 gatatagggg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tataaatagt
     9181 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttttcttt gagctaaggc
     9241 gaggcaacgc cgtactggtt tttgttaatc cactatattt tctgtccgga tacggtttat
     9301 cagggtatat caatgcggcg tatccggtgc ggaaatggat acggttggtg tcggtatgga
     9361 aacctgatgt tttcagacag catatacaaa aaaccgtact gcttgcgcgt acggtttttg
     9421 ccgtttcaaa tcggtttaaa gcgatttgag gcgggcgatt cggttgtcca gcgaagggtg
     9481 ggtgctgagc agggagtcgc gcgtatctcc ggcgatgccc attgcgttca tttcttcggg
     9541 caaatcgacc gggttgcctt tgagcctttg cagggcggaa atcattttcg gcgcgccgac
     9601 cagttttgcc gcgcccgcat cggcgcggta ttcgcgttgt cggctgaacc acatgacaat
     9661 taagctggca aggaagccga acaggatttg gaataccatg ctgaccagga aataagttcc
     9721 ctgggactgg ctgccgtcgt tgtttcgggc aatcaggttg gcaataatgc gcgacaggaa
     9781 cacgacaaag gtattgacca cgccttgaat cagcgtcagc gtaaccatat cgccgttgcc
     9841 gacgtgtgcc atttcgtgcg ccaatacggc ttccacttcg tcgcgcgtca tatggtcgag
     9901 caaaccggtg ctgacggcga tcagggagct gtttctcgat gcgcccgtgg caaaggcatt
     9961 gggttcgggg gagtggtaga tggcgacctc gggtgttttc aggttccatt gccgcgcttg
    10021 ggcttcgaca gtgttcaaaa gccaggcttc ttcttcggtg cgcggcgtgt cgatgacttc
    10081 cgcaccgacc gattgtttgg cgatgaattt ggacatcagc agcgaaataa tcgagccgct
    10141 gaagccgacg acggcggaat acgccaacag gctgcccgcg ccgccccggc tgttgatgcc
    10201 cagaaccgcc aaaacaatgt tgattacgac caaaacagcg atattggtag ccaaaaacag
    10261 aaaaatgcgt ttcacggatg ttcctttttg gtagggtgtg atgttttgaa attttgggga
    10321 ttgtcccaaa aagttgccgc cttgtgaata tcaggctcgg caaaggtatg caaaacattt
    10381 gcttgcaaat ggcagtttgt gcagttggtt tttgaactat tgtgccaagc cgtgtagaat
    10441 cgtaaaccat ctgtttgatt ccaataaaca catttaaaag gatcacttca tgaaagcatt
    10501 acttttaggc gcaccgggcg cgggcaaagg cactcaggcg caattcatca ccgcagcgtt
    10561 cggcattccg caaatctcta ccggcgacat gctccgtgcc gcgattaagg caggcacgcc
    10621 cttgggtttg gaagcgaaaa aaatcattga cgaaggcggc ttggtgcgcg acgacatcat
    10681 tatcggcatg gtcaaagaac gcatcgcgca agacgactgc aaaaacggtt tcctgtttga
    10741 cggtttcccg cgcacattgg cacaagccga agcgatggtt gaagcaggcg tggatttgga
    10801 tgcagtcgtt gaaatcgatg tgcctgacag cgtgattgtc gaccgcatga gcggccgccg
    10861 cgtgcatttg gcttccggcc gtacttacca cgttacctac aacccgccca aagttgaagg
    10921 caaagacgac gtaaccggcg aagatttgat tcagcgcgac gacgacaaag aagaaaccgt
    10981 gaaaaaacgc cttgccgttt accacgagca aaccgaagtt ttggtcgatt tttacagcaa
    11041 actggaaggc gaacacgcgc ctaaatacat caaagttgac ggcactcagc cggtagaagc
    11101 cgtgaaagcc gaagtattgg gcgcattggg caaataaatc gaaaaggtcg tacccacggg
    11161 caggcttcgc actctgaaaa cagaaaatca ggttttcaga cgacctgttt ttgataaaca
    11221 gcgtgttgca accgaaaaat aatcattt
//
LOCUS       DAXIEB010000068        11009 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000068 DAXIEB010000000
VERSION     DAXIEB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.068"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            247..429
                     /locus_tag="ACFOA3_001401"
     CDS             247..429
                     /locus_tag="ACFOA3_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898398.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNTPKSGEKRQ"
     gene            complement(478..1479)
                     /locus_tag="ACFOA3_001402"
     CDS             complement(478..1479)
                     /locus_tag="ACFOA3_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6898399.1"
                     /translation="MIDRQTNEPKQKNRITLAPMQGLIDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRATWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNRHKGGAILLKEPELIFHIVKTLRERLPAHIPLTAKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVDIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILSASEYEMNV"
     gene            complement(1660..5493)
                     /locus_tag="ACFOA3_001403"
     CDS             complement(1660..5493)
                     /locus_tag="ACFOA3_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6898400.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVGRFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLSNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGTA
                     SIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5754..5850
                     /gene="ffs"
                     /locus_tag="ACFOA3_001404"
     ncRNA           5754..5850
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFOA3_001404"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6017..7708
                     /gene="dld"
                     /locus_tag="ACFOA3_001405"
     CDS             6017..7708
                     /gene="dld"
                     /locus_tag="ACFOA3_001405"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6898401.1"
                     /translation="MSASQLLSRLTQTVGEKYIITEPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINDNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7840..7947
                     /locus_tag="ACFOA3_001406"
                     /pseudo
     CDS             7840..7947
                     /locus_tag="ACFOA3_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8003..8374
                     /locus_tag="ACFOA3_001407"
     CDS             8003..8374
                     /locus_tag="ACFOA3_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6898402.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8529..9077
                     /locus_tag="ACFOA3_001408"
     CDS             8529..9077
                     /locus_tag="ACFOA3_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6898403.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            9211..10302
                     /locus_tag="ACFOA3_001409"
     CDS             9211..10302
                     /locus_tag="ACFOA3_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6898404.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGRVLAGTGMSNTVKHLAGIEKHNLQAERADGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNRYLDDGYDSLKARLD
                     GAWAETERLAGLAWNNTPDTLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10774..10944
                     /locus_tag="ACFOA3_001410"
     CDS             10774..10944
                     /locus_tag="ACFOA3_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6898405.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2557 a         3027 c         2963 g         2462 t
ORIGIN      
        1 agtaacctat gttattgcaa aggtctcggc ttgtgcggtt tgttgaaaga aggtgctcat
       61 gagaaatccc ctaaatgtct tggtgggaat ttaggggatt ttggggaatt ttgcaaaggt
      121 ctctatttgt actgtcttcg gctcgccgcc ttgtccttat ttaaatttaa ttcactatat
      181 ttgcccgtgc gtttgtgata aattcggcac tgtatttgat atgattttaa caaatcggga
      241 ggggcaatgt tttggtatgt aatcggcttt tgcgcctttg tcgtcgcgct gctgtcgctg
      301 tgggtcaatg ccggcgcgtt cggtatgcag gaagacgata ccccgcaatc ggattatgaa
      361 aggcgtttgg gtttgggggc gaaactgaaa aacaaaaata cgccgaaatc aggggaaaaa
      421 cggcaataaa gacagcaaat gctttcagcc ggcatttgct ttgaagtatg ggagacatca
      481 aacattcatc tcatactcgg acgcgctcag gattcggcgg acttcatccg catccttaac
      541 cgttcggacg cggtcaaaca gcgtttgcgc cgggtcgaac gtttttttca tcatgcccaa
      601 ccattgctta agacgggcga tcgggtattt attgtttgcc tcttttgtca ggcacagctc
      661 gaaaaactgc cgtatccatt tagaaacttc ggcaaaatcc gtgtctttga ccggcccgcc
      721 gttctcgtat tgcttgattt gccgcgccaa atcggggcgg atgaccgcgc cgcgccccag
      781 catgacgctg ttgcagccgc tgattgtttt gatgccgata tagtcctgca ggctgaagac
      841 atcgccgttg gcggtaactg ggatatcgac gctgtctcgg attttcctta tccattccca
      901 atgcgccggc ggttcgtaac cctcggcttt ggtacgcgcg tgtacggtca gtccgcacgc
      961 gcccccttcg gcaatcgcac aggcgcattc caaagcaggg cttttgtctt catagccgag
     1021 ccgcattttt gcggtgagcg gaatatgtgc gggcaaacgt tcgcgcagcg ttttgacgat
     1081 gtggaatatc agttccggtt ctttcagaag aattgcgccg cctttgtgcc tgttgacggt
     1141 gggcgcgggg cagccgaagt tcaaatcgat tttgtccgca ccgaagcgga cggcttccag
     1201 cgcattcgcc gccatattgt ccgcatcgct gcccagaagt tggacggtgc agggcgtgcc
     1261 ggaaaacgtt ttgtttccgt tggcgatttc ggggacgtat tttaaccatg tggcgcggga
     1321 atgcacggta tgggtaatgc gtacaaattc gctgacgcat tcgtcgtagc cgcctatccg
     1381 tgtcagcagg tcgcgcatca catcgtctat cagaccttgc ataggggcaa gggtgattcg
     1441 gtttttttgt ttgggttcgt ttgtttgtct gtcaatcatg atttgggtag gtatagtgga
     1501 ttaaatttaa accagtacag cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc
     1561 ttcgtcgcct tgttctgatt tttgttaatc cactatattt ggttcaaaca atgccgtctg
     1621 aaggctttcg gcgttcagac ggcatttccg tgtccgttgt tacagcaaca ctttctctac
     1681 accgccgttg ttggcttttt ttacaaactc atccagccag ttttcgccga ggatgtattt
     1741 cgccatttcg atgacgatgt agtcggcagg catattgttg tcgtcggcgt agcggctcaa
     1801 gccttgcagg caggcggggc aggaagtcag cattttgacg ggttcgccct gcggcagctc
     1861 tttgaggttt ttctcgattt cctcttgttt gcggaacttg acctgagtgg cgatgtctgg
     1921 ccgtttgacg gcaaacatac cggattcgcc gcagcagcgg tcgcttaaaa cgactttctg
     1981 ccccatgagg ctgcttgcca tttgggtggc gttcatggtt ttgatgggcg tgtggcaggg
     2041 gtcgtggtag aggtattgct gacctttcac accgttcagt ttcacgcctt tttcgagcag
     2101 gtattcgtgg atgtcgatga tgcggcagcc ggggaagatt tcttcaaagc ggtatttttc
     2161 gagctggtcg taacaagtgc cgcaactgac gacgacggtc ttaatgtcga ggtagttgag
     2221 ggtgttcgcc atacggtgga aagccacgcg gttgttggtg ctcatttctt cggctttagc
     2281 cttgttgccg cccgcgtctt gcggatagcc gcaacacata tagccgggcg gcaggacggt
     2341 ttgtacgccg acgtgccaga gcatggcttg aacggcaagt ccgatttggc tgaacagacg
     2401 ctcagagccg caacccggga agtagaacac ggcttcggca tcttcgggcg cggcggggtt
     2461 gcggatgatg gggatgcttt tgccgtcttc gatgcccaat aaggagcgcg gtgttttggc
     2521 gggtacgctt ttcggcaaag gacggttgat gaaatggata acctgttctt tgattgaggc
     2581 agtaccgacg gttgccttgg gttcggcttt ttgctttttc gtgccgattg gaagaagttt
     2641 gccgattttg taggcaaagt tctgcgcggg gaagccggtc tgtatcatcg cggcgcgaag
     2701 ggctttgatg gttttcgggc cggtggcgtt caaaaacgcc atgcccatag ctgcggcggg
     2761 tgcaaatcgt ttgtggccgg aatcggcaag gtagttgcgg acggctacgg ttacgtcgcc
     2821 gaagtcgatg ttgacggggc agggtttgac acagcggtgg cacacggtgc agtggtcgcc
     2881 gatgtccatg agttcttcaa agtgtttgac ggaaacgccg cggcgggttt gttcttcgta
     2941 taagaaggct tcggttaaca gccctacgcc gaggattttg ttgcgcggac tgtacagcag
     3001 gttggcgcgc ggaacgtggg tggagcagac gggtttgcat ttgccgcagc gcaggcagtc
     3061 tttgacggaa tcggcgatgg taccgaggtc tgatttttcc ataatcaacg attccgcacc
     3121 taaaagctcg aaggatggcg tataggcgtt gcttaagtcc gagcctttca tcagtttgtg
     3181 acggttgaag gtgtgtttcg gatcgacttg gtttttgtag tcccaaaacg gctgcatttc
     3241 ttcgtcgctt aaaaattcga gcttggtaat gccgatgccg tgttcgccgg aaatcacgcc
     3301 gttaagcgaa cgggcgattt tcataatgcg ttcgactgag cggtatgccg tctgaagcat
     3361 ttcggcatcg tctgagttaa ccggaatatt ggtgtgaacg ttaccgtcgc cggcgtgcat
     3421 atgcagggcg acaaagacgc ggctgcgtac ggtttttgcg tggatttttc ccaagccttg
     3481 gataattttg gtgtcggttt tgccgctgaa gatttcagaa agcggtttca ttacgtccgc
     3541 tttgacagac acgcgcaggc ggaaatcgcg gaaggcaata aagcagcttt cattgttttt
     3601 ggcttcgggt gcggcgtgga cggctgcgcc gtagcgggct ttgtagtcgg caagcggcgt
     3661 gtcaagattg gcgagcagcc attcccaacg cgttttgacg gcggaaacgt gggccagggc
     3721 gtgtttgccg cgttcgccca atagttcggc ggtcggcagg tcggttccca ttttgtcgat
     3781 ggggagtttg ccggacagat attgctccaa ggcggcacag agtttgagtt tgttttgaat
     3841 ggaaagctca atgttgatgc gttcgatgcc gtctgaatac tcaccgagcc tttcgagtgg
     3901 gatgaccacg tcttcgttga ttttaaaggc gttggtatgt ttggcgatgg cggcggtgcg
     3961 gctgcggtcg agccagaagg ttttgcgcgc ttcgggcgat acggcgataa agccttcgcc
     4021 gtcgcgggcg cgggcaagtt cacagatgtg ctcggcggcg gcctctacgg cggcttcgtc
     4081 gtctgaaacc acgtccgcca gtaagaccat tttcggccgc cccttacccg ctgctttggt
     4141 ggcgtagccg acggcgcgga cgtaacgcca gtccaaatgt tccaaacccg ccagccgcac
     4201 gctgtcgtgg gcgagcagga aatcgcggat ttcgacgata gaaggcgtag cggtggcaac
     4261 cgtgccgaaa aactccatac acacggtgcg cgtgtatttc ggcattttgt gcaacacaaa
     4321 ggcgacgccg gtgatgatgc cgtctgtacc ttctttttgc acgccgggca ggccgctcaa
     4381 gaatttgtcg gtaacgtctt tgcccaaacc gactttgcgg aatttgtggc cggggatttc
     4441 caagcgttcg gttttaacga tattgatgcc gtctgaatcc agcgtgtgaa catcgaacac
     4501 ggcggtttct tcgtcgtgga ttttgccgaa attgtggcgc acgcgctcga tacgcagcca
     4561 ttcgccttga gggttgacca tctgccagta ggcgaggttg tccaacgccg tcccccacag
     4621 cacggctttt ttgccgcccg cgttcatcgc cacattaccg cccacgcaag acgcatcggc
     4681 ggaagtcgga tcgacggcga acaccaaacc tgcctgatgc gcggtttctt ccacccgccg
     4741 cgtaaccacg cccgcgccgc accggataat cggatgtttg ccatccaagc ccgccagttc
     4801 gacaaactcc acgccgcgat gtttgtccag cttttccgta ttgatgaccg cgctcattgc
     4861 gtccaaagga atcgcgccgc cggtataacc cgtaccgccg ccgcgcggga taataaccaa
     4921 atccagctcg attaaggcgc gcaccaaagg cgcgatctct gcctcggtat caggattgac
     4981 gacgacaaac ggatactcca cgcgccagtc ggttgcatcg gtaacgtgcg ttacgcgagc
     5041 caagccgtca aacataatat tgtgcggctt ggtgattttg ctcaaacgct ccaaaatctg
     5101 ccgccgcttt tggctggttt catcaaaact gctatcaaaa cgcccgaccg ccttttccgc
     5161 cgccgcaacc aagacatcga cttggcggtt gtcgtcgcgg cgtttgcgga tttcgttcaa
     5221 cctgtggcgc atttcgcgta ccagcgcggc gcggcgtttc ggatgctcca gcaaatcatc
     5281 gaccagatac ggattgcgta cgaccaccca aatatcgccc aatacttcaa acagcatccg
     5341 tgccgaacgc ccggttttac gctgtccgcg caggtcttgc aggatttgcc acgcttcgtc
     5401 gcccaataat cggatgacga tttcgcggtc ggtataagaa gtatagttgt agggaatttc
     5461 ccgaatacgc tgaggggcgg tagtcgtggt catggtgtgt ccgtgtttgg cagtttttat
     5521 ttgaatgccg tctgaaaatt cggtttcagg cgagtttgaa aaggcgataa tgtagttcaa
     5581 tttttgatat ttttcaatgc cgaaacagga aataccctta tagaaacaaa ataacgcttt
     5641 gagaaattgt tgacaatatt atcaaatata aattttaggt ggtgtttccg gcgtatgacc
     5701 gtatcaaaac ggttgagttt ttcagaaaac ccgttataat gccgtttctc tgaggcgggt
     5761 ctccccgcat ggcaaatcgg aacaccgggt caggggcgga agccagcagc ccactccgat
     5821 gcgccagtgc cgggggtttg gcccgccgcc ctatttgaaa cgccggagct ttgatgttcc
     5881 ggcgttcccg tatcgcaccg atgccgtctg aaagccgttc agacggcatt ttctttactt
     5941 tgccataacg tataactaca atgtgaaaac aattccaatg tgcaatgcac ctgccgacaa
     6001 ttttaaggaa actgccatga gcgcatcaca attactgtca agactgaccc aaaccgtagg
     6061 cgaaaaatac atcatcaccg aacccgcgaa aaccgaacaa taccgccaag gctaccgctt
     6121 cggcgagggt aaggcgttgg cggtggtgcg ccccggaagc attctggaaa tgtggaaaat
     6181 tctacaggcg tgcgtcgaag cggacgtgat tgtgattacg caggcggcga ataccggttt
     6241 gacgggcggc tcgacccccg acggcaacga ttacgaccgc gacatcgtga tagtgaacac
     6301 tatgcggatg aacatcatcc aaaccatcaa cgacaacgaa caagtcgtct gcctgcccgg
     6361 ctcgaccctg aaccagcttg aattgctgct gaaacctttg ggacgcgaac cgcattcggt
     6421 catcggctca tcctgcatcg gcgcgtccgt tttaggcggc gtgtgcaaca actccggcgg
     6481 cgcgttggta cagcgcggcc cggcctacac cgaaatggca ctgttcgccc aaatcaacga
     6541 agaaggcagg ttggaactgg tcaaccactt gggcatagac ttgggcgaca cgcccgaaga
     6601 aatcctgacc aacctgcaag gtcatcatta tcaaaacaaa gacatcaaac aagacgcggg
     6661 caaagggcac gaccacgcct attgcgaaca cgtccgccaa gtggacgaac cgaccgccgc
     6721 gcgtttcaat gccgaccccg cccgccatta cgaagcctcg ggctgcgcgg gcaaactgat
     6781 ggtttttgcc gtccgtttgg acaccttccc gcaagaaaaa caaaccgccg tgttctacat
     6841 cggcacgaac gacatcaacg agctgaccga catccgccgc gccgccttgg gcgaatttga
     6901 aagcctgcct gtttccggcg aatacatcca ccgccacgct ttcgacattg ccgacgtgta
     6961 cggcaaagac acgttttacg tcatcaaaaa attcggtacg caccaactgc cgaaattatt
     7021 tgacttgaag gcgcgcgtgg acaggttcgg caaaaaagcc agcttcctgc ccaaacattt
     7081 ttccgacaag gccatgcagt tcgtcagcaa atacctgccc gaccacctgc ccaaatccat
     7141 gcgcgattac cgcgacaaat acgaacacca cctgattctg aaaatgggcg ggaaaggcgt
     7201 ggatgaggcg cgcgcgttct taaaagaata ttttgcacac cacagcggcg cgtttttcga
     7261 gtgcaacgcc gaagaaaccc aagccgcgat gctgcaccgt ttcgccgtcg cctccgccgc
     7321 catccgctac cgcgccgtgc atgatgacga agtggaagac ttggtcgcgc tcgacatcgc
     7381 cctgcgccgc gacgatcgcg actggtttga aaaactgccg tcggaaatcg acaataaaat
     7441 catccataaa ctctactatg gacatttcat gtgccacgtt ttccatcagg attacatcat
     7501 caaaaaaggc aacgactgca tggcgttgga acacgaaatg ctgcatcttt tagaccaacg
     7561 cggcgcgcaa tatcccgccg aacacaacgt cggtcatttg tatgaagcca aacccgcgct
     7621 caaacagttt taccgcaaac ttgacccgac caacagcttc aacccgggcg taggcaaaac
     7681 cagcaaaaag aaaaactggg cggaataagc gcgtccgctt tgaaggcagg caatgccgtc
     7741 tgaaggcaaa acgccgttca gacggcattg tcgggtttga agttccccgt cgggtaatcg
     7801 ctttcaaacc gtatgccgac acacgggcgc gcccctccca aatatagtgg attaaattta
     7861 aatcaggaca aggcaacgaa gccgcagaca gtacagatag tacggcaagg cgaggtaacg
     7921 ccgtactggt ttaaatttaa tccactataa agtctgatcc taccttccca aagggcaggg
     7981 tttcaaccga aaaggaaaca cgatgaaacc atacaaaatc tacacccatc ccgccctgcc
     8041 gccccaagcc gtcaaacaag gctggtcatg gccggggctc ttgttcggca cgctgtgggc
     8101 gtgtttcaaa agaatgtggg gtttggggct ggggctgacg ggcgcgatat tcgtgctggc
     8161 tgtgtttgcc caactggttt acggcgacac gcccgccaca gactcggcgt ttaacgtatt
     8221 gggcttggcg gtttccgtct ggttcggcgc aaagggcaac agcctttatg cacgccacct
     8281 tttatcgcgc ggctataccg aattgcccga aacggttgag gcagccaatc cccaagccgc
     8341 attggcgcaa tatttcgggc gcgggggccg ataagcgcgt tttcttttcc atcatgccgt
     8401 ctgaaaaatg ttcagacggc atttcttata accttttgtt tgcagcacat aaccaatcag
     8461 tctttcctat tccgttccga aatgcatacc atgcgcccgt acccaatatt tgcgaacaag
     8521 gaaagaaaat ggcacgttta accgtacaca ccctcgaaac cgcccccgaa gccgccaaag
     8581 cgcgcgtcga ggcggtactt caaaacaacg gctttatccc caaccttatc ggcgtattat
     8641 caaacgcccc cgaagccttg gcgttttacc aagaagtcgg caagctcaac gccgccaaca
     8701 gcctgaccgc cggcgaagtc gaagtcatcc agatcatcgc cgcccgtacc aaccaatgcg
     8761 gtttttgcgt ggcagggcac accaaactcg caaccctgaa aaaactcctg tccgaacaat
     8821 ccgtcaaagc cgcgcgcgct ttggcagcag gcgaatttga cgatgccaaa ctcggcgcgc
     8881 tcgccgcctt cacccaagcc gtaatggcga aaaaaggcgc ggtatccgat gacgaactca
     8941 aagcattttt cgacgcgggc tacaaccagc agcaggcagt tgaagtcgtg atgggcgtag
     9001 ccttggcaac cctgtgcaac tacgtcaaca acctcgccca aaccgaaatc aaccccgaat
     9061 tgcaggctta cgcctgatac ggcaaaccgc ccgaatatcg ggcggttttt caacccccct
     9121 cctcaaacaa aaacaagccg ccaagcggca gggaaagcag ccgcgcatca ggcgcgcccc
     9181 cgattccccc gcaaacctta aggaacaaag atgaacgccc aaaccctgat tgccaacgtt
     9241 gccgaactcg tcaaaaccaa gctcaaaccc atagtggacg acatcgaccg caaaggatac
     9301 taccccgaag catttatgcg cgaactcggc gcaatcggcg gattcggcgc agtcgggaca
     9361 gaagccgaag gcggcaacgg cttaggcttg gcaacgcaaa tcgccgtttt gcgcgaaatc
     9421 ggcaaagaat gcggcgcaac ctccttcagc gcgtggtgtc aggcggcttg cgcgtggtat
     9481 ctgcaccaaa cgcccaacca agccgtcaaa gacaaatacc tcgccgacat cctgcaaggc
     9541 agagtattgg caggtaccgg aatgtccaat accgtcaaac accttgccgg catcgaaaaa
     9601 cacaaccttc aagccgagcg cgcagacggc ggctacacag tcaacggcgc gctgccgtgg
     9661 gtgtccaaca tcggcgaaga ccacatctgg gcaaataccg cccaaatcgg cggcggctac
     9721 gtcatgttta tcaccggcgg acaatgggaa ggcgtaagcc tgcaaaactg ccccgaattt
     9781 tgcgccctcg aaggtacgcg caccttcagc ctgaacttca aagacgtatt tatccccgac
     9841 gaagacatca tcgccgcgcc cgaacagttt gccgactaca tccaaagcat caaagcaggt
     9901 ttcatcctcc tgcaaatcgg catcggcgcg ggcgtgattg acggcagcct cggcatcatc
     9961 cgcctcgcca acatcgtcaa cgccgaagtc aaccgctatc tcgacgacgg ctacgacagc
    10021 ctcaaagcaa ggttggacgg cgcatgggca gaaaccgaac ggctcgccgg tttggcatgg
    10081 aacaacacgc cggacacatt agcgaccctc aagctgcgcg aagccgccgc cgtactggca
    10141 cttgccgccg cccaatccgc cgccctgcat tccggcgcaa aaggctacct gatgcgcagt
    10201 cccgcccaaa ggcgcgtccg cgaagcaatg tttgtcgcca tcgtaacccc cgcgatcaaa
    10261 cacctccgaa aagaaatagc ggcaatcgaa gccgcaaaat aaacaggcgg aaaaaaccga
    10321 aacccccacc gcgtcattcc cgcgaaagcg ggaatccagc ccccaacgca acaggaatcc
    10381 atcggaaaaa acagaaaccc ccaaaccgtc attcccgcaa aagcgggaat ccagccccct
    10441 aacgcaacag gaatccatcg aaaaaaccga aacctccaaa ccgtcattcc cacggaagtg
    10501 ggaatccagt aaccgaaaaa ccacaggaat ctatcgaaaa aaaccccacc ccccaaaaaa
    10561 accgggcgga tacctcacca tcctcccaaa ccctgattta tagcggatga acaaaaatca
    10621 gggcaaggcg gcgaaaccgc agacagtaca aatagtacga aaccgattca cttggtgctt
    10681 gagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ccggtttttg
    10741 ttcatccgct atacaaaaaa caaggaaaca caaatggcgc aatatatgtg cggcccctgc
    10801 ggctggattt acgatgaaga actcggcgac ccagaacacg gcatcccccc tgggacaaag
    10861 tttgaagaca ttcccgacga ctggaaatgc cccgaatgcg gcgtaggtaa agaagatttc
    10921 tacctgttgg atttcgtgat atagcggcag ccgcaccctt agggaaaacc ccgtcatccg
    10981 gcgttccgcc tgcggcggca gggaacgcc
//
LOCUS       DAXIEB010000069        10705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000069 DAXIEB010000000
VERSION     DAXIEB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.069"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..961)
                     /locus_tag="ACFOA3_001411"
     CDS             complement(<1..961)
                     /locus_tag="ACFOA3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6898406.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAVYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(1145..1864)
                     /gene="pyrH"
                     /locus_tag="ACFOA3_001412"
     CDS             complement(1145..1864)
                     /gene="pyrH"
                     /locus_tag="ACFOA3_001412"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6898407.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDSFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2083..2937)
                     /gene="tsf"
                     /locus_tag="ACFOA3_001413"
     CDS             complement(2083..2937)
                     /gene="tsf"
                     /locus_tag="ACFOA3_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6898408.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3061..3789)
                     /gene="rpsB"
                     /locus_tag="ACFOA3_001414"
     CDS             complement(3061..3789)
                     /gene="rpsB"
                     /locus_tag="ACFOA3_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6898409.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTTALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3966..4199)
                     /locus_tag="ACFOA3_001415"
     CDS             complement(3966..4199)
                     /locus_tag="ACFOA3_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898410.1"
                     /translation="MWHIVAIGYLFVVVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4277..4858
                     /locus_tag="ACFOA3_001416"
     CDS             4277..4858
                     /locus_tag="ACFOA3_001416"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6898411.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVWAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRVAQKRGAKLYLPYIEPRSQRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            4919..5509
                     /locus_tag="ACFOA3_001417"
     CDS             4919..5509
                     /locus_tag="ACFOA3_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6898412.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMYMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            6046..7512
                     /locus_tag="ACFOA3_001418"
     CDS             6046..7512
                     /locus_tag="ACFOA3_001418"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6898413.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(7699..8073)
                     /locus_tag="ACFOA3_001419"
     CDS             complement(7699..8073)
                     /locus_tag="ACFOA3_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6898414.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(8311..8610)
                     /locus_tag="ACFOA3_001420"
     CDS             complement(8311..8610)
                     /locus_tag="ACFOA3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6898415.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(8765..9544)
                     /gene="map"
                     /locus_tag="ACFOA3_001421"
     CDS             complement(8765..9544)
                     /gene="map"
                     /locus_tag="ACFOA3_001421"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6898416.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(9584..10234)
                     /locus_tag="ACFOA3_001422"
     CDS             complement(9584..10234)
                     /locus_tag="ACFOA3_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898417.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(10242..>10705)
                     /locus_tag="ACFOA3_001423"
     CDS             complement(10242..>10705)
                     /locus_tag="ACFOA3_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6898418.1"
                     /translation="DNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQV
                     ATEGEKQFVGQIARSEQAAEGVYNYITVASQPRSLGDITNDTWNTSKIRATLLGINPT
                     TQARVKIVTYGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
BASE COUNT         2247 a         2481 c         3062 g         2915 t
ORIGIN      
        1 aaggttgccc ttgtctatgt tgtcgcacta cttcatcgct gtatccatac ccctcatgac
       61 tgttatcagc ctctacggat ggggcggagt tacccgtatg attgccgtat ggccggatat
      121 cggagaaatc gaccattaat ccttctgtcg gtttaattcc tttgcttact ttatacggcc
      181 ccatccacaa tgcgtaattt tctttataga cagcttcaaa cgcattggtt tttggtttga
      241 tgagcaattt tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca
      301 gtgtttcggc attgtatagg tgcatttcgg ttctgttgcg tatcgttcca aatacgtcga
      361 tgttaataaa cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga
      421 aaaatacggt ctgcaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg
      481 tttcatttcg ataatccccc atcccgccaa tatttaagcc cagactgctc ctacttccgc
      541 taccgtctga ttgggtgcgc gagagtgcag gggcattaag tgtagataaa gaagtggtta
      601 aacctgtcaa accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat
      661 cggtacggac ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc
      721 gaccccctgt caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt
      781 ttcgtccgtg taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca
      841 caagttcttg ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg
      901 tcccgcaggc ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca
      961 tagattttct cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa
     1021 tttaaaccag tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa
     1081 tctccatata ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg
     1141 tcaatcagca gtgaaccagc gtaccttcgt tttcaccggt tactacacgt ttgagcgagc
     1201 cttctttagc aatgccgaat actacaatat tgagcttgcg ttcgcggcag agggcgaaag
     1261 cggtggcatc catcactttg aggtttttca acaaggcttc atcaaaagta atggtttcgt
     1321 agcgcgtggc ggatgagtct tttttggggt ctgcggtgta cacgccgtca acgttggtgg
     1381 ctttgagcat cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg
     1441 tgaagaacgg gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt
     1501 ggatggcttt ggggcgggcg taggtttcag cgatttgctg catagacagt gcggattgta
     1561 cgcgcgcttt gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg
     1621 tcgccatcat gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata
     1681 cgccccggaa aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga
     1741 cttcggcaat ttcgccgaca gtttgaacga tggtatcgtg attgatgccg aatgaatcgg
     1801 aacccatcag ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg
     1861 tcatgggata ccttgctttc ttgaatgtcg tcggtcggag ttgggccgtc tgaattttca
     1921 aacggtcgga attacaatga tatggtgtgt cgttctatta ttgttttgtt ttcagacggt
     1981 ctgaatattc ggtcgtctta aaataggtac cgcaaacggt tttctcaaaa aaaagcaccc
     2041 tgattcgttt ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggctgc
     2101 ggctacttcg gctgcgtagt cgacaacggc tttctcgata ccgtcgccta ctttgtagcg
     2161 tacgaagcta accacttcag tgccgttttc tttagcgaat tgggcaacag tttggtcagg
     2221 gttcatcacg aatgcttggc cgttcagagt gatttcagcc aagaatttac ggatacggcc
     2281 ttcaaccatt ttagcggcga tgtcggcagg tttgccggaa gcgatagctt gttcggtgta
     2341 gatgtggcgt tctttttcaa cggtttcagc atctacttca gcttcgctta cgcattgtgg
     2401 tttagcggca acaatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc
     2461 aaccaatacg ccttcggtcg ccaatgcgcc gtggatgtag gcaaccagtt ggttggcagt
     2521 gtcgatcact tggaagcgac ggacagacat gttctcgccc aatttggcga tgatggcttt
     2581 gcgttctgct tcaaccagtt cgctcagttc ttcaacagaa gccggttttt tctcggcagc
     2641 ggtttttgca acgaagttgg caaattctac gaagccggca tctttggcaa cgaagtcggt
     2701 ttcgcagttt acttcgacca atgcgccgac attgccgttg atcgcgtaag ccaatacgcc
     2761 ttcggcagca gtacggccgg ccagtttgcc ggctttcgcc ccggatttaa tacgcaggat
     2821 ttcttcggct ttgtcgaagt tgccttcggc ttcaaccaag gcttttttgc attccatcat
     2881 gcccaggccg gtagcggcgc gcaggtcggc aaccattttt gcagtaattt ctgccatttt
     2941 gaatctccta gatttttggg aacacggcga ttgccgtgtt tggaaatagt ggccgtctga
     3001 aacgaatttc agaacgggat ttgagaaaag gggcataacg cccctcttct gtgtaccgga
     3061 ttactcggca gcagcttctt gggcggcggc tacggtttct tgcagcgctt ggtttttgcc
     3121 ttccaaaact gcgtcagcga tgccacggca gtacaggcgg atggctttgg cggagtcatc
     3181 gttaccgggg ataacgtatt tcacgccgtc agggctgttg ttggtatcga ctacggcgat
     3241 aacggggatg cccaattttt cagcttcgac caaagtacct ttttggtagc cggtatcaat
     3301 aacgaaaatc gcgtcaggca ggcctttcat gtttttgata ccgcccaaag aacgttccag
     3361 tttttcaacg tcgcgttgca tttccagaat ttcttttttg ttgaaaccgc cttcggcagc
     3421 gttttccagg gcagtagttt tttcttccag gcgtttgatg gattgcttaa cggttttgta
     3481 gttggtcagc ataccgccca accaacggtg atcgacgaaa ggcataccgg cgcgggtagc
     3541 ttcttcgcgg atgatgtcgc gggcttggcg tttggtacct acgaacaata ctgtaccttt
     3601 gttggcaacc agacggcgta cggcttcttg cgcctcttgg aacatcggca gggttttttc
     3661 caagtttacg atatggattt tgttgcgcgc gccgaaaatg tattgtgcca ttttcgggtt
     3721 ccagaaacgg gtttggtggc cgaagtggac accggcttca atcatctgac gcatagtaat
     3781 ttgagacatt ttgatttcct tgaaagggtt aagggcacac attcaatcgc atagaacttg
     3841 ataggcgatg cctattgtca agcacccttc ggcgaaagtg ggcataagtt ttaaaaaata
     3901 aaagagtgtt tccgaaatgg aaaactgacg gattatagcg gataagaggc cgtctgaaaa
     3961 gggattcagg gttttgtgcc gctgtctttt tgccgttgtg cacgctgctg ttcgaattct
     4021 gcctgttcct gctgcttcat caagcggttg cggcggcgga cggttacgac gaagaccgtg
     4081 aacacggtgg gcagaaccgc ccaaaaaacc aaataaatca aggcgcgcgc aatactcggc
     4141 tgcgcggcgg aaaacatcac gacaacaaaa agatagccga tggcgacgat gtgccacatt
     4201 gtgtgcgtcc tgtttgaatg ttaacaaaat cgttataatc gcaacattct accgcaatcc
     4261 tgaaaggccg tctgaaatga ggaacgagga aaaacgcgcc ctgcgccgcg aattgcgcgg
     4321 gcggcgttcg caaatggggc gggacgtgtg ggcggcggca acggtaaaaa tcaaccacct
     4381 gctcaaacgt tatattaaaa aagggcggaa aatcggcgtg tattggccga tgggcaagga
     4441 attgcgtttg gacggctttg tccgtgtcgc acaaaaacgc ggcgcaaaac tttatctgcc
     4501 ttatatcgaa ccgcgttcgc agcggatgtg gtttacgccg tatcctgaaa gcggaatgga
     4561 acgggagcgc atacggggca gggcgaagtt gaacgtgccg cagtttgcag ggcgcaaaat
     4621 ccgcgtgcac ggtttgtcgg tattgctcgt cccgcttgtc ggcatagacc gcgaaggcta
     4681 ccgcttgggg caggcaggcg gctattacga tgcgacgctt gcagcgatga aataccgttt
     4741 gcaggcaaaa accgtgggcg tgggctttgc ctgccaactg gtcggcacgc tgccgcgcga
     4801 ggcgcacgac ctgccgctgg acggttttgt gtcggaggcg gggatattgt gtttttagcc
     4861 gttcagacgg cattccgggc ggaaatgccg tctgaaggcg gaatattggg agaaaaggat
     4921 gtcggaaaag ataatactgc cggtcttgtc attgggcggc gttcggctcg aaccgttgga
     4981 tgtgcatcat gaaacgggtt tgcgcgaggc ggtttgcgat ggggaggttt ggaagctggg
     5041 ggtaacgtcc gcgccccatc cggatagggt ggcagactat atcgggacag cattggcaac
     5101 gcgtttggcg tttgcggttg tcgatgaaga ggcgggcagg gtggtcggga caacggcgta
     5161 ttatcatttt gaaccgcaga ttccgcgttt ggatatcgga ttcacatggt atgcggcatc
     5221 ggcacggcgg acacggataa acacctgttg caaaatcatg ctgttggatt acgcatttga
     5281 tgttttggct tgccgttgcg tgggatggcg cacggatatt ctgaaccttg cctcgcaacg
     5341 tgccatcgaa cggctgggtg cggaaaaaga cggcgtgctg cgtatgtata tgctccgtaa
     5401 agacggcagt gtgcgcgata cggtcgtgta cagtatgctg agggaagatt ggtgcaaaaa
     5461 cagggaaatc ctgaccggca ggttggcagg gtatggcgtg caggtgtgag gcagtatttt
     5521 caaatttata gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga
     5581 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactatct gtactgtctg
     5641 cggcttcgtc gccttgtcct gatttttgtt aatccactat aaaaaactta acgcaatatg
     5701 ctgacaatct tttaaaaatt aatatataaa caattttgat tttttatcta ttaaaaattt
     5761 gatatatata gataaataac tatttttatt tttcatttcg cagtttcccg acttggcggg
     5821 cgacagaaaa actttgcatt tcccagtaaa atccactaca ttcacgataa catattacaa
     5881 gcccctgaag cggtgtcgga agccatgctt gcaacgcatt ccaaccccga aacagcatac
     5941 tcggacacac accattgccg gtttcccctg cttttgcgat tcaggcgttt gtagtacggc
     6001 aaataaccat aagggcagaa gatgcctgtt ttttaggagt aaaccatggc tgaagcgaca
     6061 gacgttgtct tggtgggcgg cggcattatg agcgcgactt tgggcgtttt gctcaaagaa
     6121 ctcgaaccgt cttgggaaat caccctgatt gaacgcttgg aagatgtggc gttggaatcg
     6181 tcaaacgcgt ggaacaacgc cggcacgggg cattccgcgc tgtgtgaatt gaactatgcg
     6241 ccgttgggtg caaatgggat tatcgatccg gcgcgcgccc tcaatattgc cgaacagttt
     6301 catgtcagcc gccagttttg ggcgacgctg gtcgcggaag gcaagttgga agacaattcc
     6361 ttcatcaatg ccgtgccgca tatgtctttg gtgatgaatg aagaccattg ttcttatctt
     6421 caaaaacgtt atgacgcgtt taaaacccaa aaactttttg aaaatatgga attttccacc
     6481 gatcggaaca aaatttccga ttgggctccg ctgatgatgc gcggccggga cgaaaaccaa
     6541 cccgtcgccg ccaactactc cgccgaaggt acggatgtcg atttcggacg gctgacgcgc
     6601 caaatggtga aatatttgca gggcaagggc gtaaaaaccg agttcaaccg ccacgtcgaa
     6661 gacatcaaac gcgaatccga cggcgcgtgg gtgctcaaaa ccgccgatac ccgcaacccc
     6721 gacgggcagc tcaccctccg tacccgcttc ctcttcctcg gcgcgggcgg cggcgcgctg
     6781 accctgctgc aaaaatccgg catccccgaa ggcaaaggct acggcggctt ccccgtgtcc
     6841 ggcctgttct tccgcaacag caaccccgaa accgccgaac aacacaacgc caaagtgtac
     6901 gggcaggctt ccgtcggcgc gccgccgatg tccgtcccgc acctcgacac acgcaacgtg
     6961 gacggcaaac gccaccttat gttcggccct tacgcaggct tccgttccaa cttcctcaag
     7021 caaggctcgc ttatggattt gccgctgtcc atccatatgg acaatctcta tcctatgctg
     7081 cgcgccggct gggcgaatat gccgctgacc aaatacctgc tgggcgaatt gcgtaaaacc
     7141 aaagaagaac gcttcgcctc cctgctggaa tactaccccg aggcaaaccc cgacgactgg
     7201 gaactcatca ccgcagggca acgcgtccaa atcattaaaa aagactccga aaaaggcggc
     7261 gtgctccagt ttggtacgga gattgtcgcc cacgccgacg gctcactcgc cgcattgctg
     7321 ggcgcgtcgc cgggcgcatc gaccgctgtg ccgctgatga tccggctgat gcaccaatgc
     7381 ttccccgagc gcgccccgtc ttgggaaggt cgtctgaaag agctggtacc gggttacggc
     7441 atcaagttga acgaaaaccc tgaaagggcg gatgaaatta tcgcctatac cgcgaaagtg
     7501 ttggatattt aagccgaatc tttcaagaaa agccgtaccc ccatatggtg ttgatgccgt
     7561 ccctgccggt tatgcccttc atccggcagg gcaggttttg gtacacgggg caggtaatcg
     7621 ttttttccct tttgtgaata atggaacagt caaaaaacag cgttttcatt tctgaaaacg
     7681 ctgttttttg ttgcctgatt aacgtgggga acagtctttg aagacgattt ggttgtcagg
     7741 tgcggtaatc ataatgggct gtttgcggta ggatttacca tccatcacgc cggtacccaa
     7801 aacataaccg ccttctttgc cgtagaatgt ttccacattg tcggatttgt ccaaattgac
     7861 aggcatttgc acgcgtttgc cgttgatgac ggcggaagcg tatgtggtca ggccctgttt
     7921 gttgaagccg taggttactt tgactttttt accttgctgg cagacgtagc tgacggtttt
     7981 tttggcaacg gtggggttgt tcgtgccggc agcagcagcc atactgctga gcgcgattgc
     8041 ggaagacagg attgcggtgg tcagaagttt catttttatt cctttggatg gatgtggagt
     8101 ttgcccgttt cgagctgttt gacattctaa tgtcggcgta tcctgaaata aacaggaaat
     8161 tggataaagt ttgtaaaaag gcgggttcgg gcggttcggc ggtttgaggc aggggcggca
     8221 ggcgggatac ggcggcctgt ccggtgtttc agacggcatt gtttgcgttt ttcggaaatg
     8281 aaaaaccttc gggcatccgg aggttgcggt ttaactttgt ttttttcttt cggacttggt
     8341 ctgtttggac aattcggcat acaggtcggg atggtgtttt ttcaacaggg cggcgatttc
     8401 catatctttt ttgttgactt tcgccaaatc gatgcattcg atatggacga ggcggagcag
     8461 ttcgcgtacg ggtttgggca ttttgcggcc ggtttcgtag cgcgagcctc cagactgggt
     8521 aacgccgacc ttgccccaga agtctatctg gttcagcccg gtctttttac ggatggcttt
     8581 aacatcttct atattttcaa atattttcat aaaacttcct ttaagtttgt ctggttgcgg
     8641 ggcaaattcc gatttgcttt gttttcgtaa tgtgccgggt caagcctgtt tttatattat
     8701 atatcatatc tgccgtttct ttcagacggc attgtttgtt tttatggggc ggatacgtcc
     8761 ggtttcaggg tttgccggag gcggggctga cggtgaggat ttcgtagccg gtttcggtca
     8821 ccaagacttc gtgttcccat tgggcggaga gggagcggtc tttggtaacc accgtccagc
     8881 cgtcgttgag gatacgcagg tggcgtttgc cttggttgat catcggttcg acggtaaaaa
     8941 tcatacccgg ttttagaacg gggccctgtc ctttttttcc gtagtgcaac acttgcgggg
     9001 cttcgtggaa accgcgcccg atgccgtgtc cgcagaattc ctgtacgacg gaatagccgg
     9061 cgttttcggc aacctgttgg caggcgtaac ctacgtcgcc cagtgtcgcg ccgggtttga
     9121 cggcttctat gcccgccatc atggaggcgt gggttacgtc gatcaggcgt tgggcgatgg
     9181 gggagacttt gccgacggta aacatacggc tggagtcgcc gtggaagccg tcttttttga
     9241 tggtgaggtc gatgttgata atgtcgcctt ctttgagcgg cttgtcgtcg ggaatgccgt
     9301 ggcagatgac gtggttgacg gaggtgcagc aggatttggg gtagggcggg ttgccgtagt
     9361 gcaggggggc gggatagccg ccttggacgt tgacgtggta gtcgtaaacg agtttgtcga
     9421 tttcgtcggt ggttacgccg ggtttgacga attgtccgat gtagtcgagg gcttcggcga
     9481 cgagtttgcc cagctcgcgc attttttcga tttcttcggg ggttttgatg atgatgccgt
     9541 tcataatgct tccttggatt gcgggtgtgc ctgccgtgcc gggtcagaag aggttgtcga
     9601 gaatttcttt atggggtttg ggcggcgtgt ttttcggggt gtccggtttg gtatggtttt
     9661 ctttgggcgt ttctttttct ttgggtgttt ctttgggctg cgcctgtttt tcgggtgttt
     9721 cttttcgggg ttcgggacgg gggacgtttg tgggcagtac gggggtttct ttgagtgtgt
     9781 tttcagcttg tgttccggcg tcgtccggct gcctgtcggt ttgagctgtg tcggcaggtt
     9841 gcggtttgac ccggtttttc ttgggtgcgg caggggacgg cagtccgctg ccgctttcgt
     9901 ctgtgccgtc cggctgtgcg ggttcggatg aggcggcgtg gtggtgttcg ggttgggcgg
     9961 catcttgtcc gactacgccg tttggcagcc agaattcggt ttcgcggggg ctgtcggtgt
    10021 gttgcggttc ggtttggagg gttttgccgc cgttcgggtt gaatgtgctg acgatgccta
    10081 ttgccaatgc gccgatggcg gtgatacaga tgagcaatac gacgcgtatc aggagtttgg
    10141 gggtcagcct gaagggtttg ttcggttttt ttgccatttt gattgtgctt ttggggtgtc
    10201 gggcaatgcc gtctgaaggc ggttcagacg gcattgccga gtcagcgttg gacgtagttt
    10261 tggtagaggg tgatgacttt ttgtacgccg acggtggtgc tgactttttg ggtaatctgc
    10321 gcctgttctt cgggggtgag gatgcccata acgtaggtta cgttgccgta ggtaacgatt
    10381 ttgacgcgcg cctgcgtggt ggggttgatg cccagcagcg tggcgcggat tttggaggta
    10441 ttccaggtgt cgttggtgat gtcgccgagg gagcgcggtt gcgaggcgac ggtaatgtag
    10501 ttgtacacgc cttcggcggc ctgttcggaa cgtgcaatct gaccgacgaa ctgtttttcg
    10561 ccttcggtgg cgacttgtcc gagcagcagc aggtggcggt tgtagccgac gacggagatt
    10621 tggggcgtgt agcccttggt ttggttgttt tggcgtaggt aggagcgggc ggtggtttca
    10681 atacgcaacg ccattacgtt gtcgt
//
LOCUS       DAXIEB010000070        10666 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000070 DAXIEB010000000
VERSION     DAXIEB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.070"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(185..1402)
                     /locus_tag="ACFOA3_001424"
     CDS             complement(185..1402)
                     /locus_tag="ACFOA3_001424"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6898419.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAVS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1604..2332)
                     /gene="rph"
                     /locus_tag="ACFOA3_001425"
     CDS             complement(1604..2332)
                     /gene="rph"
                     /locus_tag="ACFOA3_001425"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6898420.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHADGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2414..2878)
                     /locus_tag="ACFOA3_001426"
     CDS             complement(2414..2878)
                     /locus_tag="ACFOA3_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6898421.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNSARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3095..3871
                     /locus_tag="ACFOA3_001427"
     CDS             3095..3871
                     /locus_tag="ACFOA3_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6898422.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     IEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4009..4536
                     /locus_tag="ACFOA3_001428"
     CDS             4009..4536
                     /locus_tag="ACFOA3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898423.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4707..5561
                     /locus_tag="ACFOA3_001429"
     CDS             4707..5561
                     /locus_tag="ACFOA3_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6898424.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5602..6810
                     /gene="pncB"
                     /locus_tag="ACFOA3_001430"
     CDS             5602..6810
                     /gene="pncB"
                     /locus_tag="ACFOA3_001430"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6898425.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6902..8620
                     /gene="argS"
                     /locus_tag="ACFOA3_001431"
     CDS             6902..8620
                     /gene="argS"
                     /locus_tag="ACFOA3_001431"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6898426.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKIADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDSAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9148..10566
                     /locus_tag="ACFOA3_001432"
     CDS             9148..10566
                     /locus_tag="ACFOA3_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6898427.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2527 a         3031 c         2622 g         2486 t
ORIGIN      
        1 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaccact
       61 ataaatttaa tccactatat tttttgttcc aaagtcaaaa tatgccgtcc gaacattcgg
      121 gcggcagaca aaacggcact gcccgataaa ggcagtgccg ttgtccgttt caaaccgtga
      181 aacatcagcc caaattaaag gctttatgca ataccctggt tgccagttcc atgtattttt
      241 catcaatcaa tacggaaact ttgatttcgg aggtggaaat catttggatg ttgataccct
      301 cttcggcgag cgtgcggaag attttggcgg ctacaccgac gtgcgaacgc atacccaaac
      361 cgactgcaga gactttgcat acggtgtcgt cgccatcaat agaaaccgcg ccgatactgt
      421 cttggcgttc cgacaggatt tccaaagtct gcttgtaatc gccgcgcggt acggtaaagg
      481 aaaaatcggt tgtgccttcg ctgccgacat tttggataat catatcgact tcgatgttgg
      541 catcggcaac cgcgcctaaa atctgatagg cgacaccggg tttgtcgggt acgccgcgca
      601 cgttgatgcg ggcttggttt ttatcgaatg cgataccggt tacggcagct ctttccatgt
      661 tgtcgtcctc ttcaaaggta attaaggtgc cattgccgcc gtcttgcagg ctgctcagta
      721 cgcgcaggcg cactttgtat tttccggcga attctactga acggatttgc aaaactttcg
      781 aaccgaggct tgccagttcg atcatttctt caaatgtaac cgtatccatg cggcgcgctt
      841 cgggtacgac gcgggggtcg gttgtgtaaa cgccgtctac gtcggtatag atttggcact
      901 cgtcggcttt gagggctgcg gcaagcgcaa cggcggaagt gtcggaaccg ccgcgtccga
      961 gcgtggaaat atcgccttca ctgctgatgc cttggaagcc ggcaacgatg acgactttgc
     1021 ctgcatcgag gtcggcacgc attttttcgt catcaatgct ttcgatgcgg gctttggtgt
     1081 gggcggtatc ggttttgagg gcgacctgcc agcctgtgta gcttttggca tccacgccga
     1141 tgtctttcaa tgccatcgcc aaaaggccga tggttacttg ttcgccggtg gacaaaacca
     1201 cgtccaattc gcgcggatcg ggatgctcct gcatttcgtg cgccaacgcg accaggcggt
     1261 tggtttcacc gctcatggcg gatacgacga cgacgatgtc gtgtccttcg gcgcgggctt
     1321 tggcgacacg tttggccacg tttttaatgc gttcgggcga gcctacggat gtgccgccgt
     1381 atttgtgtac gattaacgcc atgtttcgtg ctttcttgtg tgggattggt cgggcagcat
     1441 ggtttgctgg aaaagcggct attattacta ttttttacat gaagttcaag aacggactgc
     1501 gctttcccac ctgccgtttg acagcggtca gcaaaaaacc tgttctttca gattgttgac
     1561 aaaatgccgt ctgaacggtt ttcagacggc atccggacga caatcaggcg gcagacagcg
     1621 cattttgctg gtgttgcagc agttcgccta tgcctttttg cgccagtgca accagtttgc
     1681 ccaattcgtc caaactgaac ggcgcgtctt ccgccgtacc ttggatttcg atgatttttc
     1741 ccgatgcggt catgacgata ttcacatcac tgtcgcaacc ggagtcttcg ggataatcca
     1801 aatccaaaag cggcacgccg ttcactacgc ctactgacac agcggcaacg gcttcgcgga
     1861 tggggttttc actcaaaatg ccgtctgaaa ccagtttgcc gacggcgatt tgcagcgcga
     1921 caaacgcacc ggtaatcgaa gccgtgcgcg taccgccatc tgcctgaatc acatcgcagt
     1981 caatcaagat ttgtcgttcg ccgagttttt ccatatccac gaccgcgcgt agcgaacgcc
     2041 cgataaggcg ttggatttct tgtgtgcgcc cggactgttt gcccgccgaa gcttcgcggc
     2101 gcatacgcga agcggtggat gcaggcagca tcccgtattc tgcggttacc cagccttggt
     2161 ttttgccgcg caaaaacggt gggacatttt cgtctatgga agcggtacaa atcactttgg
     2221 tattaccgca ttcaataagg cacgaaccgt cggcgtgcgg caggaaatga ggggtgattt
     2281 tgatatcgcg caggctgtcg gcggcgcgcg agatgcggat gtaatcaggc atactgccct
     2341 cccgttaaaa acagataaat taaaaagcct taaatatgaa aaatcacatt taaggccttc
     2401 aaactgaaaa tttctacgcc tcttcggctt tgctgcggat aatcaaaagc ggcaggtggc
     2461 tttggcgcat taccgtttcg gcaaaactgc ccattaaaag gtgcatcagc ccggtacgtc
     2521 cgtgcgtacc caacaccagc aagtcggcac cgttttcatc ggcataatca accaaatcct
     2581 gcgccatttc acgcgcaccc ttattggcaa ccagcaggtg tttgacggta ttttccacac
     2641 ccagttcccg ggcggtgcgc tcggcggcat ccaaaacttc gttgccttgc gcgacggcgg
     2701 cggcttcgta gctttcgtgt tgcaaaaatt cgggggcgag cgccatatat tcggcaggat
     2761 tggcaacgtg caccaaagtc aggcgcgcgc tgttgactcc tgcaagctcg gcggcatgtt
     2821 tcagggcatt gatggacgtt tcactgccgt caacggcaac gaccagatgt ttgtacatat
     2881 cgtattctcc ttttgcaccg cctcgcggtg ccctcttgtc ggatgggcgc agggacagtt
     2941 tgcgccgttt cattatagac ccgccgtcgg gctttataca acagccgaac agcccgaccg
     3001 ctttccagta taatatgccg cttccgtgca gtcaggcatt ttttgccggc tttcgttcac
     3061 tttttgattt gacgcaatct tgcaggattc gaccatgtcc gacaacgctt tgacctcttc
     3121 gcgacgcttc ggcggcatcg ccagactcta cggagactct gccttggcgc acttttcaca
     3181 ggcacacgtc tgcgtagtcg gcgtgggcgg tgtcggctcg tgggcgatcg aggctttggc
     3241 gcggacgggc atcggacgtt tgactttgat tgatttggac aacgttgccg aatcgaatgt
     3301 caaccgccag ctgcacgccc tgaccggaga cttcggcaaa gcaaaagtta ccgccttgcg
     3361 cgaacgcgtc gcccaaatca acccaggatg ccaagtgttt gaaattgaag atttcgttac
     3421 cgaagacaat ttgccggaat acttcggaaa aggttttgat ttcgtcatcg acgcgatcga
     3481 ccaagtgcgc gtcaaagcag caatggcggc ttattttgtg gaacgcaaac aaccgtttgt
     3541 cctcagcggc ggcgcgggcg gacaaaaaaa tccggcgtta atccaaaccg ccgatttgag
     3601 ccgcgtaacc cacgatccgc tgcttgccaa cctgcgctac accttgcgga aacgctacgg
     3661 cttcagccgc gatacgaaag ccaaaatgcg cgtgccgtgc gtgttctcaa ccgaaaacat
     3721 cacgccgccg caatcgggcg cggcgtgttc taccgatgcc gctccgcaag gcttgtcgtg
     3781 cgccggctac ggtgcaagca tgctcgttac cgcttcgttc gggctatatt gcgcacaggc
     3841 ggcggtggaa cacatcgcag acaaaaaata agcaatgccg tctgaaacag gattcagacg
     3901 gcatttgaac atactatggt tatgatttag gacaacaaag gatacggata agaaataaca
     3961 taaaatatat gattcctaat aatataccaa gtatcggaga gctatttaat ggaattcgtt
     4021 aataatttag ttattttttc atttttatta ctaatgctta ttccgatatt ttttgtagta
     4081 tatggtatat accataagat acgttatcgc aaaatatata tcctaagaat aagttttaca
     4141 ttattagtgg taatactttg cagtatgtat tacatatatt gccgttatct tgaccaacaa
     4201 aaagtagctt attattgcat agatgaacaa tgtatttcta ttgttcatct atacaaagat
     4261 tatggtataa actctcccac atatgcgaga atttacgcag gaaaaatatt gtttagattt
     4321 caagtaagag ctaaaaatta cgctgaatta cttatggaag atgatatatc aattagtaaa
     4381 aaaattttgg ggaataaatt tatcatttat gggtcgctac ctgtaatata cggtaatgta
     4441 gataatattg aagtaaaaga agctactggt tatatagata gagccagtac tgattatatt
     4501 gtctcaagaa acttaaaatt cagacattta tattaattaa gaggttttag caagaccgta
     4561 ttcacaacct gctccttttc aaaacatttg catttaaaag ccgttataat gccgtctgaa
     4621 catctgcccg accacattat gcgtgaatgt cggcagattg ttttcttttg taaacttata
     4681 ttaaaatcca cttaccgatt cacgccatgc cgcccatccc tgccccatcc gcaccatccg
     4741 agcacactgt cgcatgggta ttcggccaac ccgttaccga tttgccgcag gatttgttta
     4801 tcccgcccga cgcgctgaaa gtcgtattgg gcagttttca aggccctttg gatctactgc
     4861 tgtatctgat ccgcaagcag aacatcgatg ttctcgatat tccgatggtg aagattaccg
     4921 agcagtatct gcactacatc gcccaaatag aaacctatca gtttgatttg gcggcggaat
     4981 atcttttgat ggcagcaatg ctgattgaaa tcaaatcgcg cctgctgctg ccgcgtaccg
     5041 aaaccgtcga agacgaagaa gccgacccgc gtgccgagtt ggtgcgccgc ctgctggctt
     5101 acgaacagat gaagctggcg gcgcagggtt tggacgcgct gccccgagcc ggacgggatt
     5161 tcgcgtgggc ttacctgccg ctggaaattg ccgtcgaagc caagctgccc gaagtctata
     5221 ttaccgactt gacgcaagcg tggctgggta ttttgtctcg ggcaaaacat acgcgcagcc
     5281 acgaagttat caaagaaacc atctccgtgc gcgcgcaaat gacggcaatc ctgcgccgtt
     5341 tgaacaaaca cgggatatgc aggtttcacg acctgttcaa tcccgaacag ggcgcggctt
     5401 acgtggtcgt caacttcatc tcactgttgg agcttgccaa agaaggattg gtcagaatcg
     5461 tgcaggaagt cggtttcgga gaaattcgaa tcagcctcaa tcatgagggg gcgcattcag
     5521 acggcatttc cggcacacgg ggcgggcgcg atgtgttcta atacgcccca agccgccacc
     5581 aaaaatcggg agacacgcca tatgaccggc atcatacatt cgctgcttga caccgacctc
     5641 tacaaattca ctatgctgca agtagtcctg caccagtttc cgcagacgca cagcctttac
     5701 gaattccgct gccgcaacgc ctcgaccgtc tatccgcttg ccgacatcaa agaagacttg
     5761 gaagtcgaac tcgacgcgct ctgccggctg cgcttcaccc acgacgaact cggctatctg
     5821 cgctccctgc gtttcatcaa aagcgacttt gtcgattatc tcgaactctt ccagctccaa
     5881 cgccgctttg tcgaagtcgg cacagacgat aaaggccgtc tgaacatccg catcgaaggt
     5941 ccgatgatac aggcgatgtt ctttgaaatc ttcatcctcg ccattgtcaa cgaactttac
     6001 ttccgccgcc tggaaacccc tgcagtcata gaagaaggcg aacgccgcct tcaagccaaa
     6061 gccgcgcgcc tcaaagaaat cgccgccgca caaaaccccg acgatccgcc cttcctgatt
     6121 tccgacttcg gcacgcgccg ccgctacaag ctcgcgtggc aggaacacgt catccgcacc
     6181 ctgctcgaag ccgccccaag catcgtgcgc ggcaccagca atgtctttct cgccaaaaaa
     6241 ctcggcatta cccccatcgg caccatggcg cacgaattcc tccaagcctt ccaggccctc
     6301 gacgtacgcc tgcggaattt ccaaaaggcc gcgctcgaaa gctgggtgca cgaataccgg
     6361 ggcgatttgg gcgttgccct gaccgacgtg gtcggtatgg atgccttcct gcgcgatttc
     6421 gacctctatt tcgccaaact tttcgacggg ctgcgccacg acagcggcga cccttacgtt
     6481 tggggcgaca aagcctacgc ccactatcaa aagctcaaaa tcgacagccg caccaaaatg
     6541 ctgaccttct ccgacgggct ggacatcgaa cgctcttggg cattacacca atatttcaaa
     6601 gaccgcttca aaaccggctt cggcatcggc accaacctga ccaacgatat ggggcatacg
     6661 cctttgaata tcgtcttgaa actggtcgaa tgcaacgggc agtccgtcgc caagctgtcc
     6721 gactctccgg gcaaaaccat gaccaacaac agcaccttcc tcgcctacct gcgccaagtg
     6781 ttcgacgtac ccgaacctga aacgccgtaa accggcagaa aaagcgcaca attcctgttt
     6841 ctgccgcata aaatctttta aaataccgcc tgatttgaat ttaaccgaaa gaccgaactt
     6901 catgaaccta catcaaaccg tcgaacacga agccgccgcc gcctttgccg ccgcaggcat
     6961 cgccggcagc cccgttgtct tgcagccgac caaaaatgcc gaacacggcg atttccaaat
     7021 caacggcgtg atgggtgcgg cgaaaaaagc caaacaaaac ccgcgcgaac tggcgcaaaa
     7081 gatcgccgac gcattggcag gcaacgccgt catcgaaagc gcggaagtcg ccggtccggg
     7141 ctttatcaac ctgcgcctgc gccccgaatt tctcgcgcaa aacattcaga cggccttgaa
     7201 cgacgcgcgt ttcggcgtgg caaaaaccga caaaccgcaa accgtcgtta tcgactattc
     7261 ctcgcccaat ctggcaaaag aaatgcacgt cggccatctg cgttccagca tcatcggcga
     7321 cagcatttcg cgcgtgtcag aatttatggg caataccgtt atccgtcaaa accacgtcgg
     7381 cgactggggt acgcaatttg gcatgttggt cgcttatttg gtcgagcagc aaaaagacaa
     7441 tgccgcgttc gagctggcgg atttggagca gttttaccgc gccgccaaag tgcgctttga
     7501 cgaagaccct gcctttgccg acaccgcacg cgaatacgtt gtgaagctgc aaggcggcga
     7561 tgaaaccgtg ttagcgttgt ggaaacagtt tgttgatatt tcgctctcgc acgcccaagc
     7621 cgtttatgac acgctgggct tgaaactgcg ccccgaagat gtggcgggcg aatcgaaata
     7681 caacgacgat ttgcagcctg tggtcgatga tttggttcaa aaaggtctgg cggtcgaaga
     7741 cgacagcgcg aaagtcgtgt tcttggacga gtttaaaaac aaagaaggcg aacccgccgc
     7801 atttatcgta caaaaacaag gcggcggctt cctttacgcc tccaccgatt tggcgtgtct
     7861 gcgctaccgc gtcggcacgc ttcatgccga ccgcctgctg tacgtcgtcg accaccgtca
     7921 agccctgcac tttgagcaac tctttaccac ttcccgcaaa gcaggctatc tgccggaaaa
     7981 cgtcggcgca gcatttgtcg gcttcggcac gatgatgggc aaagacggca aaccgttcaa
     8041 aacacgcagc ggcgacactg taaaactggt cgatctgctg accgaagccg tcgaacgcgc
     8101 caccgctttg gtgaaagaga aaaatcccga attgggcgcg gacgaagccg ccaaaatcgg
     8161 caaaaccgtc ggcatcggcg cggtcaaata cgccgacttg agcaaaaacc gcaccagcga
     8221 ttatgtgttc gactgggacg ccatgctctc gtttgaaggc aacaccgccc cctatctgca
     8281 atacgcctac acccgcgtgc aaagcgtgtt ccgcaaagca ggcgaatggg atgcaaatgc
     8341 gccaaccgtt ttgaccgaac cgctggaaaa acagcttgcc gccgagctgc tgaaatttga
     8401 agacgtactg caaagcgtgg cggacacggc gtatccgcac tacctcgccg cctacctcta
     8461 tcaaattgcg accctgttca gccgcttcta cgaagcctgt ccgatactca aagccgaagg
     8521 cgcaagccgc aacagccgcc tgcaactggc aaaactcacc ggcgacacgc tgaaacaagg
     8581 cttggatttg ctgggcattg atgtgttgga cgtaatgtaa aaccgcaccg cccgattgcg
     8641 gacaacagcc ccaccatcct tatccgaatc tgaaaaaagc ggcgcgatac accgtatccg
     8701 ccgcccctcc caaaatgcga aacaaacaaa cgccaagcaa gcaagcaagc aagcaaaaaa
     8761 ttataacccc cttcctgccg acgcacgcac tttccgcgcg gcgcattccc ctttttccgc
     8821 ccctcaaatc cgccttttct tcaggcaggg tttcagcccg tctcttttcc ctgttttcct
     8881 tcccccgaca cgcgtgcgcg ccccctgccg caccgtgccg cactttcgcg cccggacggc
     8941 atcgttccgc catccggttc tctgttttaa atacccctgt ttcagaaaga aatgcagatg
     9001 tttcaacaca caggacgaca tataaagcac cgccctatgt gttgccctga tttggaaggg
     9061 gttacaccct tcctaaatat agtctgatcc tgccgccccg aaggacagat gtccgagtgg
     9121 cggggtttca accgaaaagg aaatacgatg aatattcacg ccctgctctc caaacaatgg
     9181 acgctgccgc cattcctgcc gaaacggctg ctgctgtccc tgctgatact gctggccccc
     9241 aatgcgatgt tttgggtttt ggcactgctg accgccaccg cccgcccgat tgtcaatttg
     9301 gactaccttc ccgccgcgct gctgatcgcc ctgccttggc gtttcgtcaa aattgccggc
     9361 gtattggcgt tttggctggc ggttttgttt gacgggctga tgatggtgat ccagcttttc
     9421 ccttttatgg acctcatcgg cgccatcaac ctcgtcccct tcatcctgac cgcccccacc
     9481 ccttatcaga taatgaccgg gctgttgctg ctgtatatgc tggcgatgcc gtttgtgttg
     9541 cagaaagccg ccgccaaaac cgacttccgg cacattgccg tctgcgccgc cgttgtggcg
     9601 gcagccggct atttcaccgg ccatttgagt tactacgacc gggggcggat ggccaatatc
     9661 ttcggcgcaa acaacttcta ttacgccaaa agtcaggcga tgctctacac cgtcagccag
     9721 aatgccgact ttattaccgc cggcctggtc gatcccgtct tcctcccctt gggcaatcaa
     9781 cagcgtgccg ccacgcatct gaacgagccg aaatctcaaa aaatcctctt tatcgtcgcc
     9841 gaatcttggg ggctgccggc caatcccgaa cttcaaaacg ccacttttgc caaactgctg
     9901 gcgcaaaaag accgtttttc ggtttgggaa agcggcagtt ttcccttcat cggcgcgacg
     9961 gtcgaaggcg aaatgcgcga actgtgcgcc tacggcggtt tgcgcgggtt cgcactgcgc
    10021 cgcgcgcccg acgaaaaatt tgcccgctgc ctccccaacc gtttgaaaca agaaggttac
    10081 gccacctttg cgatgcacgg cgcgggcagt tcgctttatg accgcttcag ctggtatccg
    10141 agggcgggct ttcaagaaat caaaaccgcc gaaaacctga tcggtaaaaa aacctgcgcc
    10201 attttcggcg gcgtgtgcga cagcgagctg ttcggcgaag tgtcggcatt tttcaaaaaa
    10261 cacagcaaag gattgtttta ctggatgacg ctgaccagcc acgccgacta tcccgaatcc
    10321 gacattttca accacaggct caaatgcacc gaatacggcc tgcccgccga aaccgacctc
    10381 tgccgcaact tcagcctgca cacccaattc ttcgaccaac tggcggattt gatccaacgc
    10441 cccgaaatga aaggcacgga agtcatcatc gtcggcgacc atccgccgcc cgtcggcaac
    10501 ctcaatgaaa ccttccgcta cctcaaacag gggcacgtcg cctggctgca cttcaaaatc
    10561 aaataacaac aatgccgtct gaacgcacca acagccttca gacggcattt tgcagacaga
    10621 ccgacccttc aagcccactt ttttcatcat ctccgataaa ttgctt
//
LOCUS       DAXIEB010000071        10494 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000071 DAXIEB010000000
VERSION     DAXIEB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.071"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(128..337)
                     /locus_tag="ACFOA3_001433"
     CDS             complement(128..337)
                     /locus_tag="ACFOA3_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6898428.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSEDENAV"
     gene            complement(315..755)
                     /locus_tag="ACFOA3_001434"
     CDS             complement(315..755)
                     /locus_tag="ACFOA3_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6898429.1"
                     /translation="MRHRQCGFVGEQDLQPAAGQDFFDRLAAYGVSDGIFTEQAVRIH
                     RWFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCDLLKLAGLAESGGQAKA
                     FIAEGLVSRNGETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            1080..4514
                     /gene="dnaE"
                     /locus_tag="ACFOA3_001435"
     CDS             1080..4514
                     /gene="dnaE"
                     /locus_tag="ACFOA3_001435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6898430.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNVHHAELNPEWLDNGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLVKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKSTTGDIVDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4792..8037
                     /gene="cas9"
                     /locus_tag="ACFOA3_001436"
                     /gene_synonym="csn1"
     CDS             4792..8037
                     /gene="cas9"
                     /locus_tag="ACFOA3_001436"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFC6898431.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELNLLFEK
                     QKEFGNPHISDGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPTEPKAAKNTYTAER
                     FVWLTKLNNLRILEQGSERPLTDTERTTLIDEPYRKSKLTYAQARKLLELDNTAFFKG
                     LRYGKDNADASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIDLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAATKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEEGFKERNLNDTRYVNRFLCQFVA
                     DHMLLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKAHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDES
                     ISVLRVPLTQLKLKDLEKMVNREREPKLYDALKARLETHKDNPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGGKYYLVPIYSWQVAKG
                     ILPDRAVVAFKDEEDWTVMDDSFEFRFVLYANDLIKVQLKKDSFLGYFSGLDRATGAI
                     SLREHDLEKSKGKDGMHRIGVKTALSFQKYQIDEMGKEIRPCRLKKRPPVR"
     gene            8103..9017
                     /gene="cas1"
                     /locus_tag="ACFOA3_001437"
     CDS             8103..9017
                     /gene="cas1"
                     /locus_tag="ACFOA3_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6898432.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNDESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLVVIHLYEQGRLKTELTPSIKQHLIKTLHCQTSIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKAYQYE"
     gene            9010..9336
                     /gene="cas2"
                     /locus_tag="ACFOA3_001438"
     CDS             9010..9336
                     /gene="cas2"
                     /locus_tag="ACFOA3_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6898433.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   9399..9765
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9399..9435
                     /rpt_unit_seq="tattgtagcactgcgaaatgagaatgggagctacaac"
     gene            10205..>10494
                     /locus_tag="ACFOA3_001439"
     CDS             10205..>10494
                     /locus_tag="ACFOA3_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6898434.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDEL"
BASE COUNT         2875 a         2802 c         2520 g         2297 t
ORIGIN      
        1 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
       61 tgtactgtct gcggctcgcc gccttgtcct gatttttgtt aattcactat atcaagccga
      121 accgttttca gacggcattt tcgtcttccg accaacctgc ttctttcaat ttccgccgtt
      181 gcgcgtcgta gcgggcctta tctgcccaat aaatcgtctg aatgcacgcc tcgccgcagt
      241 cgctgccgca gcattcccac gactcggggc ggacgggttc gtctaaaagc ggctcgccca
      301 aaagggcttc ggctttatgc ttcagggtcg tatccatctg cgatttccaa gcgtgcgccg
      361 tcaaactcga tgacttcgcc gccgcgtatt ttggcggttt tacgggtttc ggtttcgccg
      421 ttgcgcgaca ccagcccttc ggcgataaac gctttcgcct gtccgccgct ttcggcaagt
      481 ccggccaatt tcaagaggtc gcacaaggcg atgtattcgt tgtcttcgag atagacagtg
      541 gcttccataa tgttcccttg cagaaagagc ccgttattgt agcacctgcc gccgccgtac
      601 ccaaaattac cgcaaaacca gcgatgtatc cgcaccgcct gttccgtaaa aatgccgtct
      661 gaaaccccgt atgccgccag ccggtcaaag aaatcctgtc cggcggcagg ctgcaaatcc
      721 tgttccccga cgaatccgca ttgacgatga cgcacctcct caaacgcgaa ctgcccgata
      781 caccggcaat cggcatcaaa acgaaatcaa aaacctgttt gaacgtaaaa ttaatttaac
      841 tgcttgccat ccgtccgaat aaggtatata gttccttata actagtttga tagtccttta
      901 tatctatcaa tactccttgg gaagcctcca ccacacggta ggaggcattt ttttgccgtc
      961 gtaaaagctc aaaaacattt gcaggtcatg ccgtctgaat ctgaaacagc attacctaca
     1021 ccgccatcta aagacaaccc tgctacaata cgccttttat tgtccacgcc gattttgcca
     1081 tgaccgaacc gacctacatc ccgctgcgcc tgcataccga attttcgatt accgacggta
     1141 tggtgcggat taaaaaactg attgccaaag cgcaggaata cggtttgcct gctttgggca
     1201 tcagcgattt gatgaacgaa ttcggtttgg tgaaatttta taaagcctgc cgcagcgcgg
     1261 ggattaagcc tatcggcgcg gcggacgtat ggataggcaa tccgaatgcg cccgacaagc
     1321 cgtttcgcgc catgctgatt atccgcaacg atgcgggtta tctgcgcttg agcgagcttt
     1381 tgacggaggc ttatgtcggc aaagaccgca atgtccatca cgcggaactc aatcccgaat
     1441 ggctggacaa cggcgacaac agcggcttga tttgtttgag cggcgcgcat tacggcgaag
     1501 tgggcgtgaa tctgttgaac ggcaatgaag atgcggcgcg tgcggcggcg ttgaaatatg
     1561 cggcgtggtt tcccgatgcg ttttatctgg agctgcaacg cctgcccgaa cgccccgaat
     1621 gggaggcttg cgtttcgggc agcgtgaagc tggcggagga actgggtttg ccggtggtag
     1681 cgacgcatcc gacgcagttt atgagccgcg acgatttcaa cgcgcacgag gcgcgggtgt
     1741 gcatcgcggg cggctgggta ttgacggaca aaaaacgtcc gcgcgatttc acgccgagcc
     1801 agtttttcat tccgccggaa accatggccg aacgtttcgc cgatttgccc gaggccttgg
     1861 aaaacacggt ggaaatcgcc aaacgctgca acctgcacat cacattgggc aaaaacttcc
     1921 tgccgctctt ccctactccg gacggcctgt cactggatga ttatttggtg aaactgtcca
     1981 acgagggttt gcaggaacgc atggttcagc tttatcccga cgaggcggag cgtgcggcaa
     2041 aaatgccgga atatcaggaa cgtttggatt ttgagctgaa catcatcatc cagatgaaat
     2101 tccccggcta tttccttatc gtacaagact ttatcaactg ggcgaaaacg cacggctgtc
     2161 cggtcgggcc gggccgtggt tcgggcgcgg gttcgctggt ggcgtattcg ctgaagatta
     2221 ccgacctcga cccgctcaaa tacgcgctgc tgttcgaacg tttcttaaac cctgaacgcg
     2281 tttccatgcc cgactttgac gtggacttct gtcaggcaaa tcgcggccgc gtgattgaat
     2341 atgtgcgcga aaaatatggc gcggaagcag tcagccaaat tgttaccttc ggcacaatgt
     2401 cgtccaaagc ggtgattcgc gacgtggggc gtgtgctgga actgccgttt atgctgtgcg
     2461 acaaactgtc caagctgatt ccgttggaag ccaacaaacc cctgagtttg gaaaaagcca
     2521 tggagaccga gccgcagatt caggaattga ttgaagcgga agaagcggac gaactgatta
     2581 cgttggcgaa aaagctggaa gatttaacgc gcggtctggg tatgcacgcg ggcggcgtgt
     2641 tgattgcgcc gggcaagatt tccgattaca gccccgtgta tcaggcggac gaatccgcct
     2701 cgcccgtatc catgtacgat aaaggcgacg tggaagatgt gggtttggtg aaattcgact
     2761 ttttgggcct gcgcaacctg accattatcg aaatggcgca gaacaacatc aaaagcacca
     2821 ccggcgatat cgtcgatgtc ggcaagattc cgcttgacga ccaggttgcc taccaaatct
     2881 tccgcgatgc gaacactacc gccgtattcc agttcgagtc gaccggcatg aagaaaatgc
     2941 tgaaaacggc gcatacgacc aagtttgaag aactcatcgc cttcgtgtcg ctctaccgac
     3001 ccggcccgat ggacaacatc cccgacttcg tcgcgcgtat gaaggggcaa gaattccaat
     3061 acatccatcc gctactcgaa ggcatcctcg cgccgaccta cgggattatg gtgtatcagg
     3121 aacaagtgat gcaggcggcg cagattatcg gcggctactc gctcggcggt gcggacctgc
     3181 tgcgccgcgc catgggtaag aaaaagcccg aagaaatggt gaaacaccgc gaaatcttcg
     3241 ccgaaggtgc ggcaaaacaa ggcatttcac gcgaaaaatc cgacgaaatc ttcaactaca
     3301 tggaaaaatt cgccggctac ggtttcaaca aatcccacgc cgccgcctac gccctgattt
     3361 cctatcagac cgcatggctc aaagcccact accccgccga atttatggcg gcgaccatgt
     3421 cgtccgaatt ggacaacacc gaccagctca agcatttcta cgacgactgc cgtgccaacg
     3481 gcatcgagtt cctgccgccc gacatcaacg aatccgacta ccgcttcacg ccgtatccgg
     3541 acatgaaaat ccgctacgcg ctcggcgcga ttaaaggcac gggcgaagcc gccgtcgaat
     3601 ccatcaccgc cgcgcggcaa agcggcggca agtttaccgg cctcttggac ttctgtgagc
     3661 gcgtcggcaa agaacacatg aaccgccgca ccctcgaagc tctgatacgc ggcggcgcat
     3721 tcgacagcat cgaacccaac cgcgccatgc tcttggcgaa catcgacctc gctatggaca
     3781 acgccgacca aaaagccgcc aacgccaatc agggcgggct tttcgacatg atggaagacg
     3841 ccatcgaacc ggtgcggctc atcgacgcgc cgatgtggag cgaatcggaa aaactcgccg
     3901 aagaaaaaac cgtcatcggc ttttacctgt ccggccaccc gttcggcccg tatgcccaag
     3961 aagtccgcca aatcgccccg cagaaactgt cgaaactcaa accgcaagac agcgtgcgcc
     4021 tcgccggatt cgttaccgcc gtacgcacca tgatgggcaa gcgcggcaaa atcgccttcg
     4081 tcagccttga agatttgagc ggacaggttg aaatcatggt cggcggtcag acgttggaaa
     4141 actgcgccga ctgcctcaaa gccgaccaag tgctgattat cgaatccaaa gtcagccgcg
     4201 acgactacgg cggcggcgac ggactgcgta ttctggcaaa ccaagtcatg accctgcaaa
     4261 cggcacgcga acgctacgcc cgcagcctca gcctcgccct cgccccgcat cacgacatcg
     4321 gcggactggt acaactgctt gccgcccacc aactgcccga cacgccgcgc atcccgctgc
     4381 aactgtcgta tgccaacgaa aaagcgtcgg gcaggcttca agtgccgccg aaatggacgg
     4441 ttacgccgag ttccgcattg ttcggcgaac tggaaacatt gctcggcagc cggtcggtgc
     4501 gcgtgaattg gtaaccaaaa atacaaatgc cgtctgaagc ccaaaaccgg tttcagacgg
     4561 cattttttaa ccgaaataaa cgatgcccct taaagcagaa gctttaaggg gcagagcgtt
     4621 gcggcacatc ttttcagacg gccttattgt agcaacggtt ctcatttcgc agtgcgacaa
     4681 tatgtttggg attctagccc ttgtgagatt ataagtcaat aatctatttg tatataaata
     4741 agacaatcgg atatattatc attatttgca acgttgttta cggagtaata aatagctgcc
     4801 ttcaaaccca atccaatcaa ctacatcctc ggtctcgata tcggcatcgc atccgtcggc
     4861 tgggcgatgg tagaaattga cgaagaagaa aaccccatcc gtctgattga tttgggcgtg
     4921 cgcgtatttg agcgtgcaga agtaccgaaa acaggcgact ccctcgccat ggcaaggcgt
     4981 ttggcacgca gtgttcgccg cctgacccgc cgtcgcgccc accgcctgct tcgggcccgt
     5041 cgcctattga aacgcgaagg cgtattacaa gccgccgatt ttgatgaaaa cggcttgatt
     5101 aaatccttac cgaatacacc atggcaactt cgcgcagccg cattagaccg caaactgacg
     5161 cctttagagt ggtcggcagt cttgttgcat ttaatcaaac accgcggcta tttatcgcaa
     5221 cggaaaaacg agggcgaaac tgccgataag gagcttggcg ctttgcttaa aggcgtagcc
     5281 gacaatgccc atgccttaca gacaggcgat ttccgcacac cggccgaatt ggctttaaat
     5341 aaatttgaga aagaaagcgg ccatatccgc aaccaacgcg gcgattattc gcatacgttc
     5401 agccgcaaag atttgcaggc ggagctgaat ttactgtttg aaaaacaaaa agaatttggc
     5461 aatccgcata tttcagacgg ccttaaagaa ggtattgaaa ccctgctgat gacgcaacgc
     5521 cccgccctat ccggcgatgc tgttcaaaaa atgttggggc attgcacctt tgagccgaca
     5581 gagccgaaag ccgctaaaaa cacctacaca gccgaacgtt tcgtctggct aaccaaactg
     5641 aataacctgc gtattttaga gcaaggcagc gagcggccat tgaccgatac cgaacgcacc
     5701 acgcttatag atgagccata cagaaaatcc aaactgactt acgcacaagc ccgtaagctt
     5761 ttggagctag acaataccgc ctttttcaaa ggcttgcgct atggtaaaga caatgccgac
     5821 gcctcaacgt tgatggaaat gaaggcctac catgccatca gccgtgcgct ggaaaaagaa
     5881 ggattgaaag acaaaaaatc cccattaaac ctttctcccg aattacaaga cgaaatcggc
     5941 acggcattct ccctgttcaa aaccgatgag gacattacag gccgtctgaa agaccgtgta
     6001 cagcccgaaa tcttagaagc gctgttaaaa cacatcagct tcgacaagtt cgttcaaatt
     6061 tccttgaaag cattgcgccg aattgtgcct ctaatggaac aaggcaaacg ttacgatgaa
     6121 gcctgcgccg aaatctacgg agaccattac ggcaagaaga atacggaaga aaagattgat
     6181 ctgccaccca tccctgccga cgaaatccgc aaccccgttg ttttgcgtgc cttatctcag
     6241 gccagaaaag tcattaacgg cgtggttcgg cgttacggct cccccgcacg tatccatatt
     6301 gaaacggcaa gggaagtagg aaaatcgttt aaagaccgca aagaaatcga aaaacgccaa
     6361 gaagaaaacc gtaaagaccg tgaaaaagcc gccaccaaat tccgagagta tttccccaat
     6421 tttgtcggcg aacccaaatc caaagatatt ctgaaactgc gcctgtacga gcaacaacac
     6481 ggcaaatgcc tgtattcggg caaagaaatc aacttaggcc gtctgaacga aaaaggctat
     6541 gtcgaaatcg accatgccct gccgttctcg cgcacatggg acgacagttt caacaataaa
     6601 gtgctggtat tgggcagcga aaaccaaaac aaaggcaatc aaacccctta cgaatacttc
     6661 aacggcaaag acaacagccg cgaatggcag gaatttaaag cgcgtgtcga aaccagccgt
     6721 ttcccacgca gtaaaaaaca acggattctg ctgcaaaaat tcgatgaaga gggctttaaa
     6781 gaacgcaatc tgaacgatac gcgctacgtc aaccgtttcc tgtgccaatt cgttgccgac
     6841 catatgctgc tgacaggtaa agggaaaaga cgtgtctttg cctcaaacgg acaaattacc
     6901 aatctgttgc gcggcttttg gggattacgc aaagtgcgtg cggaaaacga ccgccatcac
     6961 gccttggacg cggtcgtcgt tgcctgctcg accgttgcca tgcagcagaa aattacccga
     7021 ttcgtacgct ataaagagat gaacgcgttt gacggcaaaa ccatagacaa agaaacagga
     7081 gaagtgctgc atcaaaaagc acacttcccg caaccttggg aatttttcgc acaagaagtc
     7141 atgattcgcg tcttcggcaa accggacggc aaacccgaat ttgaagaagc cgatacccca
     7201 gaaaaactgc gaacattgct cgctgaaaaa ttatcttctc gtcccgaagc cgtacacgaa
     7261 tacgttacac cactgtttgt ttcacgcgcg cccaatcgga agatgagcgg gcaaggccat
     7321 atggagaccg tcaaatccgc caaacgactg gacgaaagca tcagcgtgtt gcgcgtaccg
     7381 ctgacacagt taaaactgaa agacttggaa aaaatggtca atcgggagcg cgaacctaag
     7441 ctatacgacg cactgaaagc acggctggag acgcataaag acaatcctgc caaagccttt
     7501 gccgagccgt tttacaaata cgataaggca ggcaaccgca cccaacaggt aaaagccgta
     7561 cgcgtagagc aagtacagaa aaccggcgta tgggtacgca accataacgg tattgccgac
     7621 aacgcaacca tggtgcgcgt agatgtgttt gagaaaggcg gcaagtatta tctggtcccg
     7681 atttacagtt ggcaggtagc gaaagggatt ttaccggata gggctgttgt tgcttttaaa
     7741 gatgaagaag attggacggt aatggatgat agttttgagt ttaggtttgt actttatgcg
     7801 aatgatttaa ttaaagttca acttaaaaaa gacagctttt taggttactt ttcaggatta
     7861 gatcgagcca ctggagccat ttctcttcgg gagcatgatt tggaaaaatc aaaaggtaaa
     7921 gacggtatgc atcgtatcgg cgttaaaacc gccctttcct ttcaaaaata ccaaatcgac
     7981 gaaatgggca aagaaatcag accatgccgt ctgaaaaaac gcccacctgt ccgttaaatt
     8041 aatctatccc tgtttcagac ggcctgaagc agggattttt atataaaaat aaggggaaaa
     8101 acatgacttg gcgcagcctg ctcattcaaa acggcggcaa actatcctta caacgccggc
     8161 aactgctgat tcaacaaaat gacgagtccc acaccgtacc attagaagac atcgccgtta
     8221 tcatcatcga aaaccgcgaa accctgatta ccgctccact tctgtcagcc ttagccgaac
     8281 atggcgcaac cttactgaca tgtgacgagc agttcctgcc ctgcggacaa tggctgcctt
     8341 atgcccaata tcaccgccaa cttaaaatcc tcaaactgca gctgaatata agcgaaccct
     8401 taaaaaagca attctggcaa catatcgtcc gccaaaaaat cctgaatcaa gcatttgtcg
     8461 cagacgaaac cggcaatgat cttgcagcca aacggctgcg cactttggca tctgaagtcc
     8521 gctcgggcga tacaggaaac cgcgaagccc aagccgccgc cctctacttc caagccctct
     8581 ttggcgaaaa atttacccgc aatgacaaca acgccgtcaa tgccgcattg aactatacct
     8641 acgctgtgct ccgggctgca gttgcacgcg ccctgacctt atacggttgg ttgcctgcat
     8701 taggcttgtt tcatagaagt gagttgaatc cattcaatct cgccgatgac tttatcgaac
     8761 ccttacgccc attggccgat ctggtcgtta tacatttgta cgaacaaggc cgtctgaaaa
     8821 ctgaactgac gcccagcatc aaacaacacc ttatcaaaac ccttcactgt caaaccagca
     8881 tcgaacggca acatttcagc accttggctg ccatcgacaa aatggtttcg tcatttcaag
     8941 ccggcgtaac tgataaaaac gccaaacaac tgaaactgcc tgaaatccta ccattgaaag
     9001 cataccaata tgagtgaggc caaatttatg aggattatcg tcttcttcga cctaccggtt
     9061 atcacggcgg caaagcgcaa agccgccaat caattccgcc agtttttatt aaaagacgga
     9121 taccaaatgc tgcaactttc cgtatacagc cgtatcgtca aaggccgcga ttcgttgcaa
     9181 aaacaccaca atagactatg tgcaaacctt ccgcaagaag gctcaatccg ctgtttggag
     9241 ataacagaaa agcaatatgc cgccatgaaa ctgctgttgg gcgagctgaa aacccaagaa
     9301 aaaaaggtca attcagacca attattgtta ttttaagccc attttttcag actaaataaa
     9361 acgggaaatc cttatagaat aaggatttcc cgttggggta ttgtagcact gcgaaatgag
     9421 aatgggagct acaacaagtt gattacattt acggtgcaga cggttattgt agcactgcga
     9481 aatgagaatg ggagctacaa ctcgtcatgc ggtcagagtt gccgaacaca cattgtagca
     9541 ctgcgaaatg agaatgggag ctacaacgtg catggtattg gtttgttttg gtaaattatt
     9601 gtagcactgc gaaatgagaa tgggagctac aacttttgat tttttatatt gagcgttttt
     9661 gatattgtag cactgcgaaa tgagaatggg agctacaacg tattttttcc gacccccctg
     9721 acccccctta ttgtagcact gcgaaatgag aatgggcgct acaaccagcc gttgcgataa
     9781 gcgaacgtgc gttaaagtca ttgagacaca gtgcaattat ggcaagaaag gcgacagata
     9841 ttgtgtcaca gatttgaaat aagaaaaaac cgcccactca cagagtaggc ggaaaacttg
     9901 cgtttttttg attgtggtcg ggttagctac tgcattgact taacagcaaa gcaaccgcta
     9961 aaatgaactt gtggttcact tcaacggaca gcagcaactt tttcccacgc ataaggaatt
    10021 taagcatttt agcttctcct agtgaagtta atcacacagg cgagagccga acaaaaatcc
    10081 ccgttgcagc ggggattttt gctgttccct gtttgatgat taaactaaaa tcttgcaaaa
    10141 tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
    10201 tctcatgagc accttctttc agcaaaccgc acaagccatg attgccaaac acatcgaccg
    10261 tttcccgcta ttgaagttgg accaggtgat tgattggcaa ccaatcgaac aatacctgaa
    10321 ccgtcaaaaa aaccgttacc ttagagacca ccgcggccgt cccgcctacc cgctgttgtc
    10381 catgttcaaa gccgtcctgc tcggacaatg gcacagcctc tccgatcccg aactcgaaca
    10441 cagcctcatc acccgcatcg atttcaacct gttttgccgt tttgacgaac tgag
//
LOCUS       DAXIEB010000072        10464 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000072 DAXIEB010000000
VERSION     DAXIEB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.072"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(150..743)
                     /locus_tag="ACFOA3_001440"
     CDS             complement(150..743)
                     /locus_tag="ACFOA3_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6898435.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(748..1095)
                     /locus_tag="ACFOA3_001441"
     CDS             complement(748..1095)
                     /locus_tag="ACFOA3_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6898436.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1142..2017
                     /gene="rsmI"
                     /locus_tag="ACFOA3_001442"
     CDS             1142..2017
                     /gene="rsmI"
                     /locus_tag="ACFOA3_001442"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6898437.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            complement(2052..2447)
                     /locus_tag="ACFOA3_001443"
     CDS             complement(2052..2447)
                     /locus_tag="ACFOA3_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6898438.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(2575..2685)
                     /locus_tag="ACFOA3_001444"
                     /pseudo
     CDS             complement(2575..2685)
                     /locus_tag="ACFOA3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            2963..4117
                     /gene="obgE"
                     /locus_tag="ACFOA3_001445"
     CDS             2963..4117
                     /gene="obgE"
                     /locus_tag="ACFOA3_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6898439.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            4200..5249
                     /locus_tag="ACFOA3_001446"
     CDS             4200..5249
                     /locus_tag="ACFOA3_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6898440.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            5937..7358
                     /gene="cysS"
                     /locus_tag="ACFOA3_001447"
     CDS             5937..7358
                     /gene="cysS"
                     /locus_tag="ACFOA3_001447"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6898441.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            7441..8220
                     /locus_tag="ACFOA3_001448"
     CDS             7441..8220
                     /locus_tag="ACFOA3_001448"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6898442.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            8207..8551
                     /locus_tag="ACFOA3_001449"
     CDS             8207..8551
                     /locus_tag="ACFOA3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898443.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            8562..9092
                     /locus_tag="ACFOA3_001450"
     CDS             8562..9092
                     /locus_tag="ACFOA3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6898444.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(9321..10436)
                     /gene="asd"
                     /locus_tag="ACFOA3_001451"
     CDS             complement(9321..10436)
                     /gene="asd"
                     /locus_tag="ACFOA3_001451"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6898445.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2601 a         2634 c         2809 g         2420 t
ORIGIN      
        1 ccgattactg cgctgacgca gccgctaagg gcaaggctga aaatggcggc ggtcaggatg
       61 cggacggtgt gcagtttgag tttcatcggg ggcttccttt cttgggcgtt tcagacggca
      121 ttgctttgcg ccatgccgtc tgaaaagggt tacattcctt ccagcagtac ggagtcgata
      181 cagtcgcaca tggcgtgtat cagcaggatg tggttttcct gaatacgggc agttcgagga
      241 tagggaacgt tgagtaaaac gtcggtgtct ttgagtatgg cggcgatttt gccgccgtcg
      301 cggccggtca gggcgatgac gtgcatatcg cgttcgtgtg cggctttgac ggcttcgatg
      361 acgttggcgg aattgccgga agtggaaatg ccgacgagaa cgtcgccggc gcgtccgagg
      421 gcgcgtactt gtttgctgaa gacgtggtcg aagccgtagt cattgccgat ggcggtcagc
      481 gcggaggtgt cggtggtcag cgcgacggcg gcgagttcca tacgttcttt ttcaaaacgc
      541 ccggtcattt cggcggcgaa gtgctgtgcg tcggcagccg aaccgccgtt gccgcacgcc
      601 aggattttgc cgtcattcat caggcattgc agcatcagct cggcagcctg tacggtcggc
      661 tcgaccagta ctttttcggc ttcttgcttg gcacggatgc tttcggcaaa atgggcggca
      721 acgcgttctt gtaatgtcgt catatcgtca acctgtaata ttctgtatcc actcgggcgg
      781 gcggctgcct tcgatgagta ccgcgtcgag gcggcacggt acgtttgtca acctgttctg
      841 ttgcagataa tactctacac ttcgttgcag tttcaataat ttggatgggg aaatgctgta
      901 tgcgacaccg ccgaattgcc gatttttgcg gtattttact tcaacaaaca gaatcatgcc
      961 gccgtttttg acaatcaggt cgatttcgcc gtaggcgcag tgccagttgc gggcaagcag
     1021 tgtgcagcct tgggattgga ggaaggcaag cgcggcatct tcgcctgcct cgccctgttt
     1081 gtggtttagg cgcataatca gtttaacctt tataatgtct gtttttcaga cggcattttt
     1141 tatgtttcag aaacacttgc agaaagcctc cgacagcgtc gtcggaggga cattatacgt
     1201 ggttgccacg cccatcggca atttggcgga cattaccctg cgcgctttgg cggtattgca
     1261 aaaggcggac atcatctgtg ccgaagacac gcgcgttacc gcgcagcttt tgagcgcgta
     1321 cggcattcag ggcaaacttg tcagcgtgcg cgaacacaac gaacggcaga tggcggacaa
     1381 gattgtcggc tatctttcag acggcatggt tgtggcacag gtttccgatg cgggtacgcc
     1441 ggccgtgtgc gacccgggcg cgaaactcgc ccgccgcgtg cgtgaggtcg ggtttaaagt
     1501 tgtccctgtt gtcggcgcaa gcgcggtgat ggcggctttg agtgtggccg gtgtggcggg
     1561 atccgatttt tatttcaacg gttttgtacc gccgaaatcg ggagagcgca ggaaactgtt
     1621 tgccaaatgg gtgcgggcgg cgtttcccgt cgtgatgttt gaaacgccgc accgcatcgg
     1681 tgcgacgctt gccgatatgg cggaactgtt ccccgaacgc cgattaatgc tggcgcgcga
     1741 aattacgaaa acgtttgaaa cgttcttaag cggcacggtt ggggaaattc agacggcatt
     1801 gtctgccgac ggcaaccaat cgcgcggcga gatggtgttg gtgctttatc cggcgcagga
     1861 tgaaaaacac gaaggcttgt ccgagtccgc gcaaaacatc atgaaaatcc tcacagccga
     1921 gctgccgacc aaacaggcgg cggagcttgc cgccaaaatc acgggcgagg gaaaaaaagc
     1981 tttgtacaat ctggcactgt cttggaaaaa caaatgatgc cggacggacc ggcaccatag
     2041 tgcgaacgga atcaacggac acgccccgtc atgcggtaca gcacgtcttt gccttgaaca
     2101 cggtggacga cgaccatggc gacgtgtccg acgacggcgg caaacagcag ccagcccaaa
     2161 ttgccgtgga acgtgttgcc caagtttgcc atccactcga ttttttccgg cgaaccctgc
     2221 atcacctcaa cgccgaacac tttcaacggg ccgcggccgc tgccgtattg gcggatcatg
     2281 ccgataacgg gaacggcaag catgagcaga tacagaatgc cgtgtcctgc cgccgcagcc
     2341 ttgctgtcgc tttgcggacg cttggcgcgg ttggcaactg cccacacgat gcgcaatgta
     2401 atcaccgtca gcgtgaggaa gccgaaagat ttgtgcaggc cgaacaggct gcccgcccat
     2461 tccgcctctt cgtatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta
     2521 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaat
     2581 cgtccacagg acaatggtgg tcagaatgcc gatgaaaccg gcaacggtca gccagtgcag
     2641 cgcgcgtgtc aacgggtcgt aaacggcagg ttggtttgtg ttcataaaaa acttattata
     2701 tgtaacggtt tgaacggaca ttccgtcggg tcggaatgtc aaaaaggcgc gattgtacca
     2761 aagaagttgg acataatttt gtttgcaggc tgaagatttg cttaaaaatt cattaagatg
     2821 ggcggaacaa atagtttggg tacggcgtgt tgaaataccg cttatccctt ataatagcgt
     2881 ccgaaattct gttcagacgg catcaaaaca cacggtaatc cgtctgaacc ccccatttga
     2941 aaccaacaaa caagagcatt gaatgaaatt catcgacgaa gcaaaaatcg aagtcgccgc
     3001 aggcaaaggc ggtaatggcg caaccagttt ccgccgcgaa aaattcgtac cgcgcggcgg
     3061 cccggacggc ggcgacggcg gcaaaggcgg tagcgtctgg gcggaagccg acgaaaacac
     3121 caacaccctc gtcgaatacc gcttcgtcaa acgataccaa gccaaaaacg gcgaaaaagg
     3181 ccacggttcc gaccgttacg gcgcaggtgc ggacgacatc gtcctcaaaa tgcccgtcgg
     3241 caccctcatc cgcgacctcg acaccgacga aatcgttgcc gacctcactt accacggaca
     3301 gcgcgtctgc ctagccaaag gcggcaaagg cggcttgggc aacatccact tcaaatcctc
     3361 cgtcaaccgc gccccaaaac aatccacgcc cggcgaagaa ggcgaagccc gttccctgca
     3421 actcgaactc aaagtcctcg ccgatgtcgg cttattgggt atgcccaacg ccggcaaatc
     3481 caccctgatt accgccgtat ccgccgcacg ccccaaaatc gccaactacc ccttcaccac
     3541 cctgcatcca aacttaggcg tggtacgcat cgacgaaaac cacagcttcg tgatggccga
     3601 catccccggc ctgattgaag gcgcggcaga aggcgcgggt ttggggcatc gtttcctcaa
     3661 acacttatcg cgcaccggat tattgctgca cgtcgtcgat ttggcgccct tcgacgaaac
     3721 cgtcaaccct gccgaagaag cacttgctat catcaacgaa ttgcgcaaat acgatgaaga
     3781 actttacggc aaaccgcgct ggctggtgct gaataaactc gacatgcttg acgaagaaga
     3841 agcccaaacg cgcactgccg ccttcctcga agccgtcggc tgggactacc ccaaacccga
     3901 cgaccgtttc caatttgaca tggaaacccc gcgcctcttc caaatcagcg cgctgaccca
     3961 ccaaggcacg caggaattgg tacaccaaat caaccaatac ctgaccgaga aaaaacgtat
     4021 cgaagctgaa aaagccgaag cggagaaagc agcggcaaat gttgaaatta tcgaacagca
     4081 gcctaaaacg gatacgggcg tgtttaagcc ggagtgaaaa aagaaagggt ttcagacgat
     4141 atttgttcta caaaaggttg tttgaaacaa ttttaaagca agattgaaat ggattaaaaa
     4201 tgtcatctaa ccaatatatc cagtcgcttg agttgacaaa ctttaccgtt cttccaaacg
     4261 atacttttga attttctgaa aatctcaatg ttattgttgc cgaaaacggt tgtggcaaaa
     4321 cacatttgtt aaaaatttta tacagcctgc tagaagtaac atctaacacc aaaaacagat
     4381 tactgaaaac agaattgcaa aaaagttttg ccgataaatt actgaatgtt ttccgtcccg
     4441 attccttggg caggttgagc aaacgtttgc aggggcgcgg gcgtacggaa atcgttttaa
     4501 aactgcaaaa cggcactacg catagtagtc tgaatttttc cagtaactca tcatcgcaag
     4561 tcaatgttca aagcatcgga ttgaaagaaa atgaatatac tccgacacct atcttcttac
     4621 ccagccgcga attaattaca ctttgccctt ggttcacttc tttgtatcaa aaccaaagca
     4681 tccctttcga agaaacttgg tttgatacct gtatgcaatt aaatcacccg cttgccaaag
     4741 gccccagaga aacaaaaatt cgagaattgc tcgaacctat tgagaacgcg atgggcggaa
     4801 aagtatctga agaacaagga aggttttatc tatcgctgac aaatacgggc ggtaagattg
     4861 aagcaccttt ggtagcagag ggtttgcgga aatttgtcat gattgcccgg ttaatcgcaa
     4921 caggggcatt gctggataaa ggctatctat tttgggatga accggaagct aatctgaatc
     4981 ctaaattgat caaagtggct gcccgtatta tttggtcttt atctcaacag ggtattcagg
     5041 tttttattgc aacacactct ctattcttat tgagagaatt ggaacttttg aagcttgaaa
     5101 ataacacgga tttacctgta cgttttttct cgttgattgc ttccgatgat ggaacaaaag
     5161 tggagcaagg gaacagtatt aatgatttga acacattgat attgttagat gaaaacatta
     5221 tgcaggcaga caggtatctg aatatttgag gttaagctat gactacaatc acagaaaaga
     5281 gattaacctt tgcttttcct gaagattatt acgttaccaa gtatgacgaa tgggaacatt
     5341 ataaaatttt tcagaacagt tgtaatttac gtaataaaat tgatacaaat gaaaagggaa
     5401 agaacgggat aaatcaaagt gtagatgacg ataatggcag ctcaggtgta gatataatag
     5461 cccttcatga gtcgacactt tggctaatcg aaattaaaga ttattaccga cttgggcttg
     5521 agccaaatgc ccaatcaata gatgaaaaac tttctgacct accatattta atcgcaagaa
     5581 aaattcgaga ttcattggca ggattggttt ctgctaaatt taaagcagaa aaacaggaag
     5641 aaaaagattt ttctcgcttg gctttagatt gtaacgaaat aaaaatagtc cttcatattg
     5701 aaatgcctaa tataagaagt aaactttatc cctcgtcatc agatttagca aacttattaa
     5761 aagataagtt taaattatca gaatttacaa aaaattttgc aaactgttat gcagaaccaa
     5821 ttttcacaaa tatcaaccat attaataatc cgcaactacg taatgttcct tggtccgtat
     5881 ctacaggaac agaacagaaa ttatcatccg agcaacaaag attaatccat aatcccatga
     5941 ccaccatcta caacaccctt acccgccaaa aagaaccctt tgcccccatc gaccctgaaa
     6001 acgtgcgtat gtacgtttgc ggcatgactg tttacgacta ttgccactta ggccatgccc
     6061 gtgtgatggt ggtgttcgac atgattgccc gttggttgcg tgagtgcggt tatccgctca
     6121 cttatgtgcg caacatcacc gacatcgacg acaaaatcat cgcccgtgcg gctgaaaacg
     6181 gcgaaaccat cggcgaactg accgcgcgtt tcattcaggc tatgcacgaa gatgccgatg
     6241 ctttaggtgt gttgcgtccg gatattgagc cgaaggcgac ggaaaacatt ccgcaaatga
     6301 ttgccatgat tgaaaccctg attcaaaacg gcaaggcata tcctgccgca aacggcgatg
     6361 tttactacgc cgtgcgcgag ttttccgctt acggccaatt gtcgggcaaa tcgttggacg
     6421 acctgcgcgc aggcgagcgc gtggaggtgg acggtttcaa acgcgatccg cttgattttg
     6481 tgttgtggaa agcggccaaa gcaggcgagc cggcatggga aagcccgtgg ggcaatggcc
     6541 gtccgggttg gcacattgaa tgctccgcca tgagtgaaaa cctgttcggc gatactttcg
     6601 acatccacgg cggcggtgcg gatttgcagt ttccgcacca cgaaaatgaa atcgcccaaa
     6661 gcgtcggtgc cagcggtcat acctgcggcc acgaccacgc gcaaacccat cacggtcaaa
     6721 gcatcgccag ccacgtcaaa tactggctgc acaacggctt catccgcgtg gatggcgaaa
     6781 aaatgtcgaa atcgctgggc aacttcttca ccatccgcga agtattgaaa caatacgacc
     6841 ctgaagttgt gcgtttcttc atcctgcgcg cccactaccg cagcccgttg aactattccg
     6901 acgcgcattt ggacgacgcg aaaggcgcgt tgacccgtct atacaccacc ttgaaaaaca
     6961 cgcctgccgc cgcgtttgat ttgtctgaaa acgccaacga ctacacccgc cgcttctacg
     7021 ccgccatgaa cgacgatttc ggtacggttg aagcggttgc cgtgttgttt gagctggcag
     7081 gcgaagtgaa caaaaccaat gacgcacagc tcgccggctg cctgaaagcc ttgggcggca
     7141 tcatcggact gctgcaacgc gacccgaccg agttcctgca aggcggcgcg gcttcagacg
     7201 gcctctccaa cgaagaaatc gaagacttga tcgcccggcg gaaacaggcg cgcgccgata
     7261 aaaactgggc ggaatccgac cgcatccgcg accttctgaa cgaacacaaa atcattttgg
     7321 aagacaacgc cggcggcacg acttggcggc gcggttaaaa tccatcgaaa agggtaaatg
     7381 tgccgcattt tgcccttttt tgttcagacg gcatttttat tgtgagaact tatttttatt
     7441 atgcttaaaa tcatttccgc caacgtcaac ggcatccgct ccgcctacaa aaaaggtttt
     7501 tacgaataca tcgccgcatc gggcgcggac attgtctgcg tgcaggaact caaagcgcag
     7561 gaggctgatt tgtctgccga tatgaaaaat ccgcatggga tgcacggcca ttggcattgc
     7621 gccgagaagc gcggttacag cggcgtggcg gtgtacagca aacgcaaacc cgacaatgtg
     7681 caaatcggta tgggcattga agaattcgac agggaagggc gatttgtgcg ttgcgatttc
     7741 gacaggttga gcgttatttc gctttacttg cccagcggca gcagcgcgga agaacgccag
     7801 caggtgaaat accgtttcct tgatgcgttt taccctatgc tcgaagcgat gaaaaacgaa
     7861 ggacgcgaca ttgtcgtttg cggcgactgg aacatcgccc accaaaacat cgacctgaaa
     7921 aactggaaag gcaatcagaa aaattcaggt ttcctgccgg aagaacgcga gtggataggc
     7981 aaggttatcc acaagctcgg ttggacggat atgtggcgca cgctttatcc cgatgtgccg
     8041 ggctacacgt ggtggagcaa ccgcgggcag gcgtatgcga aagatgtcgg gtggcgcatc
     8101 gattatcaga tggttacgcc cgaacttgcc gccaaagccg tgtccgcaca cgtttataaa
     8161 gatgaaaaat tttccgacca tgcgccgctg gtcatggagt atgactatgc tgccgaataa
     8221 agttttgggt aaatatgatt ggaatgtgga cggaaaaaca ggaataggcg cagcatgggt
     8281 tgtcgctgcg tttatcctgc ccatattggt gtgggcgata tttatgttgt cgcggatgca
     8341 aggctggctt gcgccgacca aggcaaaccc gacatgggcg ttggtgtggc tgctgatctg
     8401 catgccctgc ctgctgattg cgtccaaatg tttgggttgg aaaggctggc ggcgggtggt
     8461 gaatattttt gcctgcctga ccgtctgcgc catattgagc gtacccgctt cgctgctcat
     8521 cgcctttacc ctccgggacc tgctgaaata ggcggggcgg tatgcagggt tttgaactcg
     8581 aagatttgac gctttggctg atacgcgatg ccggagagga tcagatgtgg atagaccgtt
     8641 gggcgatcag ttatcccgtc gtgcagatgt ccgaagcgtc ggccggtcaa agcatagggg
     8701 aatggcaggc agggcttcgg acggcatttg aacgcatacg tggcaaatac gtcgccgttg
     8761 tcgcacacgg tgcgggcgcg gccgcatttt tggcgtggct gtatcaggca gacatcctga
     8821 cacggaagaa aattgccaac atcatccttg taccgcagcg tcccgatatt tttcccgacg
     8881 atgcggaaca cactttccaa cgcgtccgct gtccctgccg tgccgcattg gtggtgtccg
     8941 aacacgggga tgtgccgcga gattgggcgc aaaaacaggc ggatacgtgg aacgcgcgcc
     9001 tgttggtttc cccgcattcg ggcagtttga acggtatgct cggcggctgg cagtggggta
     9061 tgaagctgat gcaggaaatg ttgctggcgt gaacgcttgc cttatcggaa tttcggctgt
     9121 agcaggaagt ccgcatatcg ggctgttttc attgacgtgc aggcgcatca ggttcggata
     9181 aagtcgggct gccgcatacc gccggaaatg gaatccttta ttttttacgt cggcagtgcc
     9241 ggcagggcgt tatccggcag ggttgcaagg aaaatgccgt ctgaaacagg cttcagacgg
     9301 catttccatt caaacagggc ttacaggctg cccaacacga tacgcaatac gcggcgcagc
     9361 ggctcggccg cgccccacaa gagctggtcg ccgacggtga acgcgctgat gtattcgccg
     9421 cccatgccca gtttgcggat gcgtccgaca gggacggaca gcgtgccggt aactttggca
     9481 ggagtcagct cgtggatgct ggcttctttt tcattgggga tgactttcac ccagtcattc
     9541 gcgcctgcca aaatcgtttc gatttcggaa acaggcaggt cttttttcaa cttcagagtg
     9601 atggcttggc tgtggcagcg catcgcgccg acgcggacgc acaggccgtc aatcacggtt
     9661 ggattgtcgc tgcggccgag gattttgttg gtttccacgc cgcctttcca ttcttctttg
     9721 gactggccgt tgcccaaatc cacgtcaatc cacggaatca ggctgccggc gagcggtacg
     9781 ccgaagttgg ctttcggata gtcttcgctg cgcaggaaat cggatacttt gcggtcgatg
     9841 tcgagaatcg agccggcagg atcggcaagt tcgtccgcca cttgggcgtg aaccgcgccc
     9901 ataccgctga tgagttcgcg catgtttttc gcacctgcgc cggaagcggc ttggtaggtc
     9961 atgctggttg cccattcgac caaatcgttt tggaacaggc cgcccaaagc catcagcatc
    10021 agggagacgg tgcagttgcc gccgatgtag tttttcacgc cgtttttgag gccgttgtcg
    10081 ataacgttgc ggttgacagg gtcgaggacg ataatcgcgt cgtctttcat gcgcagagaa
    10141 gatgccgcgt caacccagta gccgttccag ccgctgtcgc gcagggcttg gaagacggat
    10201 ttggtgtaat cgccgccttg gcaggtaacg atgatgtcca ttttggcgag ttcggcaaca
    10261 ttgttggcat ctaataatgt tttagccgcc tgaccgaaat caggggctgc gccgccgacg
    10321 ttggaagtgg taaagaaaaa cgcttcagga atgtgggcga agtcgttttc ttctttcata
    10381 cgctgcatca aaaccgaacc gaccataccg cgccagccga cgaaacctac tttcattgtg
    10441 ttgctccttg aggaaaaatc gtta
//
LOCUS       DAXIEB010000073        10159 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000073 DAXIEB010000000
VERSION     DAXIEB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.073"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            481..1938
                     /locus_tag="ACFOA3_001452"
     CDS             481..1938
                     /locus_tag="ACFOA3_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6898446.1"
                     /translation="MSRHPAPTGEKAFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2419..2778)
                     /gene="rnpB"
                     /locus_tag="ACFOA3_001453"
     ncRNA           complement(2419..2778)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFOA3_001453"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2857..3960)
                     /gene="prfB"
                     /locus_tag="ACFOA3_001454"
     CDS             complement(2857..3960)
                     /gene="prfB"
                     /locus_tag="ACFOA3_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6898447.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFTAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(4063..4956)
                     /locus_tag="ACFOA3_001455"
     CDS             complement(4063..4956)
                     /locus_tag="ACFOA3_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6898448.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKLVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5299..5748
                     /locus_tag="ACFOA3_001456"
     CDS             5299..5748
                     /locus_tag="ACFOA3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6898449.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLTMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            5822..6130
                     /locus_tag="ACFOA3_001457"
     CDS             5822..6130
                     /locus_tag="ACFOA3_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898450.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            6246..7088
                     /locus_tag="ACFOA3_001458"
     CDS             6246..7088
                     /locus_tag="ACFOA3_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6898451.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            7078..7824
                     /locus_tag="ACFOA3_001459"
     CDS             7078..7824
                     /locus_tag="ACFOA3_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6898452.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETLQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLTQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            7846..8433
                     /locus_tag="ACFOA3_001460"
     CDS             7846..8433
                     /locus_tag="ACFOA3_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6898453.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8439..8618
                     /locus_tag="ACFOA3_001461"
     CDS             8439..8618
                     /locus_tag="ACFOA3_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6898454.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8850..9290
                     /locus_tag="ACFOA3_001462"
     CDS             8850..9290
                     /locus_tag="ACFOA3_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6898455.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(9819..10159)
                     /locus_tag="ACFOA3_001463"
                     /pseudo
     CDS             complement(9819..10159)
                     /locus_tag="ACFOA3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2620 a         2716 c         2285 g         2538 t
ORIGIN      
        1 attttgcaaa ggtctccgac ggtctttata gcggattaac aaaaatcagg acaaggcgac
       61 gaagctgcag acagtacaaa tagtacggaa ccgattcact cggtgcttga gcaccttaga
      121 gaatcgttct ctttgagcta aggcgaggca acgccgtacc ggtttttgtt aatccactat
      181 aatgcgccct tcggcgtggc ggatatataa ggaagtgatt ttccatctaa gtaaaaaccg
      241 ccctatcgga taagccctta acagaaaagg ctttacccgc gccgtatcgg aacacgtact
      301 ctaaaataca atccattgaa ttgaaaaaaa tataaaaaca tccgcccgcg aaaaacggca
      361 gcgcgtcgtt tgacaaagaa tgaaaatatc ggttaaaaac cgattttcat acaaaaaaca
      421 ccgctgccgt ccgcatccgt ttcagacggt attgagagaa aatcttttag gagaaccttt
      481 atgtcccgac atcccgcccc caccggagaa aaagcattct tcggccaccc cttccagctt
      541 tccaccctct tccatatcga attgtgggaa cgtttttcat tttacggaat gcagggcatc
      601 ctgctgattt acctctacta caccgccgac aaaggcggct tgggcataga caaaaccctc
      661 gccggcggca ttgtcggcgc atacagcggc agcgtgtacc tgtccaccat tttgggggcg
      721 tggtttgccg accgcgtatg gggtgcggaa aaaaccctct tcctctcggg catcgtcgtg
      781 atgctcggac acatcgtcct tgccgcagca ccgggcctgt acggcctttt aatcgggctg
      841 atattcatcg cattgggcag cggcggcgtg aaatctacgg ccagttctat ggtgggcgca
      901 ttatacgaac aggacgaaat gcgcccgctg cgcgatgcgg gattttccat tttctacatc
      961 gccatcaata tcggcggctt cctaggcccg ctgctgaccg gcctgctgca ggaaaacatc
     1021 ggtttccatt atggtttcgg tgcggcggcg gtcggtatgg cattcggctt gtggcgttat
     1081 tccctgggac gtaaaaacct gccccacccc accgtccccc atccgctttc aaaaggacag
     1141 ggcaaaactg cggccgccgt cggcatcgcc ctcatcgccg cacttgcaac cgccatcaaa
     1201 accgggcttg tcaacctcga caatttctcc ggcatcctct tatctaccgt catccttgcc
     1261 gtcatcgcct atttcgcccg cctgctgact aatccccgcg tcagttccga caacaaacgg
     1321 cacatcatcg cctacatccc gcttttcctg accatctgta tgttttgggc ggtctggttt
     1381 cagatttaca ccgtggcaac cgtctatttc gacgaaaccg tcaaccgcac catcggttcg
     1441 tttaccgtgc ccgtcgcctg gaaagattct atgcaaagcc tgtgggtcat cctgttttcc
     1501 ggactgatgg cggcaatgtg gacaaaaatg gggcgcaaac agcccaaaac cccgctgaaa
     1561 ttcgctatgg cggtatttgt taccggcgcg tcgtttttgg gattcgtccc ctttatttcc
     1621 gccggcacgc cgatgcctat tgcggttttc gcactgattg tccttgccat cacgataggc
     1681 gaactgatga tttccccgat tgcactgtcc atctccacca aaatcgcacc gcctttattc
     1741 aaaacccaaa tggtcgccct taatttcctt gccttttcat taggcttcac tttgggcggc
     1801 gtattgtttg aaaaaggcta tcaggccggc gacgaaatcg gcttctaccg gctgctgttc
     1861 tacatcggcg cagccacagg cttcctgctg ctcctgctcg tccccaaatt gaacaaaatg
     1921 ctcgaaggca cagactaagt cctaccccga tgcgccgatg ccgtctgaac ccttcagacg
     1981 gcatttttcc cgcataatga aaccaaaccg tttccacccg acaggacagg ctccccccaa
     2041 ccggaaggca gcctgccgat tgtcatttaa atatctgact gattccgtct gtttgcaagg
     2101 gaaatagccg tttatttccg cttgggcgga aaccggttct attgagtaaa aggcatttta
     2161 tccgactaaa ttagtgctgc atcaacggaa tatatagtgg attaacaaaa atcaggacaa
     2221 ggcgacgaag ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcaacac
     2281 cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc
     2341 cactataaaa acacaaccta aataaaaatg ccgtctgaac cgtatttcag gtttcagacg
     2401 gcatttgcgt gtcggatgca caccggacgg gcggtaagcc gggttctgtc tcggacagtc
     2461 attcctctag gcataccgtt gccggtatgc tcaagcaacc tacccgaacg ctcggcgggc
     2521 agcgtcattg cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt
     2581 accaaatgcg cggtgcgccc ttaccgcacc ttttcaccct tgcctgtact gccaaagcag
     2641 ccatcggcgg ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac
     2701 cggcattcta ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc
     2761 gactgtctgc ccgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct
     2821 gaaacacaaa atgcttcaga cggcatacgg ttcaaactac acgccctgtt tcaggctggc
     2881 ttcgataaag ccgtccaaat cgccgtccaa tacggctttg gtgttgccta cttcgtagcc
     2941 tgtgcgcaag tctttgatgc gcgaggaatc caaaacatac gaacggattt ggctgcccca
     3001 acctacatcg gatttacctt cttccaacgc ctgtttctct tcgttgcgtt tgcgcatttc
     3061 caattcatac agtttggatt tcaacatttc catcgcggca gctttgttgg cgtgttgcga
     3121 acggtcgttt tgacactgca ccacaatccc cgtcggctcg tgggtaatac gcacggcgga
     3181 gtcggtttta ttgatgtgct gaccacccgc acccgatgcg cggtaggtat cgatacgcaa
     3241 atcggcgggg ttgatttcga tttcgatgga atcgtcgatt tcagggtaaa cgaacacgga
     3301 ggcaaacgag gtatggcgtt tgttgttcga gtcaaacggc gaatagcgca ccaagcggtg
     3361 aacgccggtt tcggtacgca gcaaaccata agcgtattcg ccttccacgc ggatagtggc
     3421 gcggttgatg cctgcgattt cgccgtcgtc ttcttcgagg atttcgattt tgaagccttt
     3481 gcgctcggcg tagcggctgt acatacggaa cagcataccc gcccagtctt ccgcttcggt
     3541 accgcccgcg ccggcggtga tgtcgataaa gcagttgttc gggtcggcgg gctggttgaa
     3601 catccgtttg aactccaaat ccgccatctg tttttccagc cccgccacat cttcctgcac
     3661 ggcggtaaaa ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc
     3721 ttcgatgccc gaagcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc
     3781 tttgccgatt tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc
     3841 gataacttct tccaatcggt ctttcttgcc ctgataatcc atataaacgc ggatgtcttc
     3901 gctgcgcttt tccaaatcgt tcagggtatt gttgagctgg ttgattactt cggcttccat
     3961 gattcttttg ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc
     4021 tatggataaa gccttctgga aacacgttcg gacggcatag cgtcaataac ggtatgccgc
     4081 cagtttgcgt ttgatttcag gcaatgcggc acgtgctgcc tcctcgccca accggatggc
     4141 gcgttttttc tgatcgaatc cgccgactgc acccaaatcc aaaacctgtg gtttgataac
     4201 tacatccgcc tgccccaact cattttgcaa cgcagaaacg ctcattacgt tcagcgtctg
     4261 atcgagataa gagaagaaac cttggctgat gtttttgccc ggacgggcgg aaatatcgac
     4321 ggcaatcacg aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag
     4381 accgccgtca acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc
     4441 ggcggaagcg cgcacagcct gcccggcatt cccctgattg aaagcgacgg ccttgccggt
     4501 ttcaaaatca gtagcaacgg cggcaaattt gatgggaaac tgctgaatct gcctgccgcc
     4561 gacttttcgg ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa
     4621 ggttaaatcg accaaatcgg ttttgcctaa aatttcggct tccaattcga ggcggtcggg
     4681 cgacataccc gatgcaaaaa ggctgccgac aatcgaacct gccgatgtac cggtaaccac
     4741 cttcacagga ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt
     4801 agatgcgccg ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccag
     4861 cttgcgggca gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat
     4921 tgccaaaagc ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcac
     4981 accccgtcaa aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg
     5041 ggactttgtt aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat
     5101 ttgaaggcat actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc
     5161 cgcctttttc cctttctttc catcaaaata ctttcttttt atattcatta acttgttaaa
     5221 tcattggctg ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa
     5281 ccaaaacagg aacacttaat gaaaatcgga acaacttggc agacggcatc cgctatgctg
     5341 gttttgcgtc tgtttgccgc atatgaattt ttggaatcgg gtttgcaaaa atggaacggg
     5401 gagaattggt tttccgaaat caacgatcag tttccattcc cgttcaactt gctgccggac
     5461 gcgttaaact ggaatctcac catgtatgcg gagcttttac tgcccgtact gttgcttttg
     5521 ggtttggcaa cgcgtctgtc ggcattaggg ctgatggtcg ttaccgccgt cgcttgggct
     5581 gcggttcacg ccggttcggg ttacaatgtc tgcgacaacg gttataaaat ggctttaatt
     5641 tatatcgtgg tattaatccc gctgcttttc cagggtgcgg gcggatggtc gctggatacg
     5701 ctgctgaaaa aacggttttg cccccaatgc cgtctgaagc aagattgatt cagtcgtgga
     5761 atctgacttt aaacattcca accttatctc gttaacttga tattctgaaa aggaaataga
     5821 aatgaacaaa aacattgctg ccgcactcgc cggtgcttta tccctgtctt tggccgccgg
     5881 tgcagttgct gccaacaaac cggcaagcaa cgcaacaggc gttcataaat ccgcccatgg
     5941 ctcttgcggc gcgtccaaat ctgccgaagg ttcgtgcggc gcggctggtt ctaaagcagg
     6001 cgaaggcaaa tgcggcgagg gcaaatgcgg tgcgaccgta aaaaaaaccc acaaacacac
     6061 caaagcatct aaagccaagg ccaaatctgc cgaaggcaaa tgcggcgaag gcaaatgcgg
     6121 ttctaaataa tcccacccct tcaaaccaag ccgcgttttt cagtaaaatg cggctttttt
     6181 aacggcaaac aaagattttt taacaagcac atcattcttt tgtgccatcc gaaccgggta
     6241 aaaatatgat tcaacacgca ggcttgggct accgccgcga cttggcggaa gactttctct
     6301 cgctttccga aaacagcccg atatgcttta tcgaagccgc accggaaaac tggctgaaaa
     6361 tgggcggctg ggcgcgcaaa cagtttgacc gtgtggcgga acggctgccg ctggcgttgc
     6421 acggattgtc tatgtcgctg ggcgggcaag caccgctgga tactgatttg atagacggca
     6481 tcaaagaaat gatgcgccgt tacgattgca cgtttttctc cgaccatttg agctactgcc
     6541 acgacggcgg tcatctttac gatttgttgc cgctgccctt taccgaggaa atggtgcatc
     6601 atacggcgcg gcgtatccgc gaagtgcaag accgtttggg ctgccgcatc gccgtggaaa
     6661 acacgtccta ctatctgcat tccccgcttg ccgagatgaa cgaggttgag ttcctcaacg
     6721 cggttgcacg tgaggccgat tgcggcattc atctggacgt gaacaatatc tacgtcaacg
     6781 ccgtcaatca cggcctgttg tcgccggagg cttttttgaa aaacgttgac gcagatcgcg
     6841 tgtgctatat ccatattgcc ggacatgacg tggaaacgcc ggaattgttg attgatacac
     6901 atggcgcggc agttttgccg actgtttggg acttgctcga acttgcctat gccaagctgc
     6961 cgacgattcc gcccaccctg ttggaacgcg attttaattt cccgcctttt gccgaactcg
     7021 aagccgaagt cgccaaaatc gccgattatc aaacgcgtgc cggaaaggaa tgccgccgtg
     7081 cagcctgaaa cctccgccca ataccagcac cgtttcgccc aagccatacg cgggggcgaa
     7141 gccgcagacg gtctgccgca agaccgactg aacgtctata tccgcctgat acgcaacaat
     7201 atccacagct ttatcgaccg ctgctatacc gaaacgctgc aatactttga cagcgaagaa
     7261 tggagccgtc tgaaagaagg tttcgtccgc gacgcgcacg cccaaacgcc ctattttcaa
     7321 gaaatccccg gcgagttcct acaatattgc caaagcctgc cgctttcaga cggcattttg
     7381 gcactgatgg attttgaata tacccaattg ctggcagaag ttgctcaaat tccggatatt
     7441 cccgacattc attattcaaa tgacagcaaa tacacaccct cccctgcggc ctttatccgg
     7501 caatatcgat atgatgttac cgatgatttg catgaagcgg aaacagcctt gttaatatgg
     7561 cgaaacgccg aagatgatgt gatgtaccaa acattggacg gcttcgatat gatgctgctg
     7621 gagataatgg gttcctccgc gctttcgttt gacaccctca cccaaaccct tgtcgaattt
     7681 atgcctgagg acgataattg gaaaaatatt ttgcttggga aatggtcagg ctggactgaa
     7741 caaaggatta tcatcccctc cttgtccgcc atatccgaaa atatggaagg caattccccg
     7801 ggccaaaacc atctatccgc ataaaattac cttgttcccg atactatgcc gctacccgac
     7861 ctgaccgatg ccgaattaat agagtcgcgt aaactgcttc tgcattttgc gcggcttcag
     7921 ttgcccgacc accctgattt ggctgaagat ttagtgcagg aaacattgct gtccgcatac
     7981 agcgcaggcg acagttttca aggcagggca cttgtcaaca gctggctttt tgccatattg
     8041 aaaaacaaaa ttattgacgc attacgtcaa atcggaaggc agaggaaagt ctttaccaca
     8101 ctggatgacg agctactgga tgaagcattt gaaagccatt tttcccaaaa cgggcattgg
     8161 acgccggaag ggcagccgca acattggaac actccggaaa aatcattaaa caacaacgaa
     8221 ttccaaaaaa ttctgcaaag ctgcctatac aacttgcctg aaaacaccgc acgggtattt
     8281 accctgaagg aaatactcgg tttttcatcc gacgaaatac aacaaatgtg cggtatcagc
     8341 acgtccaact accacaccat tatgcaccgc gcccgagaat cattgcgcca atgcctgcaa
     8401 atcaaatggt tcaaccaaga aaacccgaag taaacgttat gaaaaaatgc cgcgatatcg
     8461 ccctgcttct ttccaaacat caggaccggg aaaccacccc gggcgagaag atttccatat
     8521 acacacacct gctgttctgc ccacattgcc gagaatataa aagacagctt caaaccatca
     8581 aaatatcact ggcaaaaaca accagaactt caaaataaat gccgtctgaa aaggcttcag
     8641 acggcataag ctgacggaaa caaatcaaac cgatttactg ttatcggcag ttcatccata
     8701 atacacgctt caaaagcagc atattcctcc atacggaata tataaatacg caaaacacga
     8761 aggctgcatc aatttgcttt atttgcctta tttcacagac ggcgctaccc ctcccgccca
     8821 acccgttctt tctgaatgag cagatttcaa tgattaagga aaccctaatg cgcccaatct
     8881 tcctatcttt cgttttactc cctattttga taaccgcctg cagcacaccg gacaagtctg
     8941 cccgatggga aaatatcggc acaatctcaa acggcaatat tcatacatat atcaataaag
     9001 acagcgtgag aaaaaacgga aatctgatga ttttccaaga taaaaaagtt gttaccaatc
     9061 taaaacaaga acgttttgcc aacacccccg catacaagac tgccattgcc gagtgggaaa
     9121 tccactgcaa caacaaaaca taccgcttaa gttcgctaca gttgtttgat acaaaaaaca
     9181 cggaaatttc cacacaaagc tacacagcct cttccctccg cccgatgagc atcctgtccg
     9241 ggacattaac cgaaaaacaa tatgaaaccg tatgcggaaa aaaactctga ttgcaactta
     9301 tatacaaact tacccacaaa ccttatcata aaaatgccgt ctgaaatact gaaatatcag
     9361 catttcagac ggcattttgc cattccctga aaattatcca caaagttatc cacattattt
     9421 tttaaaaccg gcttccatcc gaaatatagt ggattaacaa aaatcaggac aaggcgacga
     9481 agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtactgg tttaaagtta
     9541 atccactata taaactcgct atacaatttc actatccaaa cgtaaattgt tccattgata
     9601 cacaaaactt cttaccccca taattttgat aaagcatttc ttacattccc ggctccgtcc
     9661 cgtaaccaac acagcctcta aaaaataatc gcttgttttg acaccgatac actcatatag
     9721 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
     9781 ggcgaggcaa cgctgtactg gtttaaattt aatccactat acaaatacag aaactcaaga
     9841 aaataacctt gtgtattgac catctcaagc aattcagaaa aatcaagaaa ttttctgacc
     9901 gtaaacaaac gtttccctaa aaaaaacgat gtcttcaaaa atatcgacca aataatcatc
     9961 ttcgccgttt tcatttttat gggttaaaac aacacgggaa aaatctgttt tcagatgctt
    10021 gcccgcttga ttgttcggat tattgtccgg aacgacaaaa ccgtcctcaa aatcaaagta
    10081 gacgttgcgt ccttctacct ttatctctgt gcaataacaa tcatgcagag aaataccatc
    10141 cgaaaaattt aggggctgt
//
LOCUS       DAXIEB010000074        10086 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000074 DAXIEB010000000
VERSION     DAXIEB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.074"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(108..1781)
                     /gene="recN"
                     /locus_tag="ACFOA3_001464"
     CDS             complement(108..1781)
                     /gene="recN"
                     /locus_tag="ACFOA3_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6898456.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQKQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1965..2567)
                     /locus_tag="ACFOA3_001465"
     CDS             complement(1965..2567)
                     /locus_tag="ACFOA3_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6898457.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2668..3609)
                     /gene="ylqF"
                     /locus_tag="ACFOA3_001466"
     CDS             complement(2668..3609)
                     /gene="ylqF"
                     /locus_tag="ACFOA3_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6898458.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDTRMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3690..4544)
                     /gene="rapZ"
                     /locus_tag="ACFOA3_001467"
     CDS             complement(3690..4544)
                     /gene="rapZ"
                     /locus_tag="ACFOA3_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6898459.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4525..5487)
                     /gene="hprK"
                     /locus_tag="ACFOA3_001468"
     CDS             complement(4525..5487)
                     /gene="hprK"
                     /locus_tag="ACFOA3_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6898460.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGNLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5491..5940)
                     /gene="ptsN"
                     /locus_tag="ACFOA3_001469"
     CDS             complement(5491..5940)
                     /gene="ptsN"
                     /locus_tag="ACFOA3_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6898461.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6125..7015)
                     /gene="ubiA"
                     /locus_tag="ACFOA3_001470"
     CDS             complement(6125..7015)
                     /gene="ubiA"
                     /locus_tag="ACFOA3_001470"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6898462.1"
                     /translation="MNPKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7045..7521)
                     /locus_tag="ACFOA3_001471"
     CDS             complement(7045..7521)
                     /locus_tag="ACFOA3_001471"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6898463.1"
                     /translation="MEHLFGKWLPDLPADVSDGISLPMVRLLHTRSLTAALRALPHLF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSVFEFAVFGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7536..8183)
                     /gene="bioD"
                     /locus_tag="ACFOA3_001472"
     CDS             complement(7536..8183)
                     /gene="bioD"
                     /locus_tag="ACFOA3_001472"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6898464.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLIFNHIHDSSDECVAQDSLNYLQCRLKDSFPEAEWLELDKTDAV"
     gene            complement(8180..9481)
                     /gene="bioA"
                     /locus_tag="ACFOA3_001473"
     CDS             complement(8180..9481)
                     /gene="bioA"
                     /locus_tag="ACFOA3_001473"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6898465.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVEAQIKQMSHVMFGGLTHDPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            9698..9813
                     /locus_tag="ACFOA3_001474"
                     /pseudo
     CDS             9698..9813
                     /locus_tag="ACFOA3_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9811..10038)
                     /locus_tag="ACFOA3_001475"
     CDS             complement(9811..10038)
                     /locus_tag="ACFOA3_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898466.1"
                     /translation="MNVWDIWSRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVL"
BASE COUNT         2370 a         2737 c         2516 g         2463 t
ORIGIN      
        1 cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc tgagttctgt
       61 ctattgtttt agacttttat ttttagttat tttttaactg attgatttta aaataaacta
      121 tttttcgacg caagttgcag caattctgcc gcatgttgcc gcgtcgtatc ggtaatgact
      181 tctccgccca acatacgggc aacctcttcg atccgttgga tttcatccaa tatactgatt
      241 tcgctgacgg tttgctctcc ctcgctgtgc ttgcgcaccc gccagtggtt ttcgccgcag
      301 gatgcgactt gcggaagatg ggtaacggca agcacctgat gttttctgcc caacgcacgt
      361 aatgccttgc cgaccatttc agccactccc cctccaatac cggtatcgac ctcatcaaaa
      421 atcagggtgg gaacttgggt atattggctg gcaacaacct gtaaggcaag gctgatacgc
      481 gccaattcgc cgccggaggc aactttattc agcggacggg gcggattgcc tttgttggcg
      541 gcaacttgaa attgaacctg ctccaaaccg tgtgccgtcg gcgacgaagg caacaggacg
      601 atgtcgaaac gtgcaccttt catggcaagg tgttgcatat gctcggtcgt ttcgccgctc
      661 aaacgctctg ccgcctgatg gcgcatggca gaaaggatgt gggcggcttc ctgatattcg
      721 gcaaaattgt gggcaacatt atgctcgagc gcgtccaaat cggcggcagc ttgcaggctt
      781 tgcaggcgtt cttcgatttc tgccaacttg gcaggcaact cttcaggttc gatccggtat
      841 tttcgcgcca tccccatcag ttcgcccata cgctgttcct gtgccgccaa ttcattggga
      901 ttgatgtcgc tccaacctgc cacatcgcgc atattggcac tgatttcgcc caattcggct
      961 tcgatgcttg ccaacatatt caggctctcg gcaaagcgcg gctcgatgtt ttgcagattg
     1021 gccaatagtt tttgacattg atagatatgg cgttggatgc cgttgtcgcc gtcaatcttg
     1081 cttccgactt cttcggcggc ctgcaacagt tcggcagaat gggcaaggct gtcgtggctt
     1141 tggctgaggg cttcccactc gccttgttta atgtccaact gattcaattc gttaaactgc
     1201 cattccagac gctcttgttc gagtgcaatg ttttctgcct gtttctgcgc ttcctgcaat
     1261 gctttttttg agtttgccca gttttgataa agctgcctga cggtttccgc ctgcgccctg
     1321 ctgcccgcaa atgcgtccaa caattcgcgc tgggcggctt cctgattaag cgaatgatgg
     1381 gcgttttgcc catggatgtc gataagctgg ctgccgacgg ctttgagttg cgccaaggta
     1441 gcggcctgat tgttgataaa gctgcggctt ttgcctttgg catcgataat gcggcggata
     1501 ctgagttctt cttctccgtc gtttaaaagc ccctgttcat acaattctgc ttttaaaaca
     1561 ggtaaatggg aaatatcaaa caacgccgac aactgcgctt cttttgcgcc gctgcggact
     1621 tggctgtaat cggctttatc gcccaacagc agaccaatcg catccaaagt aatggacttg
     1681 cccgcgccag tttctccggt caatacggta aagccgcttt gaaaatccag attcagattt
     1741 tcaacaatga caaaatcacg caaagaaagt gttagaagca ttgtgaggat tccttagcaa
     1801 ttctaccgac aaaagcaata tcaactttag atatacgaat tataactaag attttttaaa
     1861 ttaaaaataa gtttttcctt aatttttctt agtgcttgtt tttatctgct tatttgttgc
     1921 aatgccgtct gaagcaatgt gcgtttcaga cggcatttgg aatttcagtt gggcaaggta
     1981 tcggacggta cggtttccgg ctcttcttca tccgtaggcg gcatttctat caaatcgggc
     2041 aaaaccagtt cgcccagttc ggtcagcggc ggcagttctt ccaagctgtt caaacccaaa
     2101 tcgctgagga acgttgccgt tgtcgcccac aatgcgggtt ttcccaatgt gtcccgatgt
     2161 ccgataactt caatccaccc ccgatcctgc aaggtctgca tcacgttctg cgacaccgcc
     2221 acgccgcgta tgccttcaat gtcgccacgc gttacgggct gctggtaggc gataatcgcc
     2281 agtgtttcca tcacggcgcg ggagtaacgc ggcgcacgct gttcttgcag gctgcccagc
     2341 cgctcgaatg ccgtctgaac aatctgaaaa cgccagccct cttgcgtatg caccagttgc
     2401 aacgccctat cctgccaacg cgttttcaac tgcgccaaca catcaatcag tttgtcctgc
     2461 gacagtggcg gcacacacaa ttcgcgcata gatttttcgg tcagcggttc ggtttgggtc
     2521 agcagtgcgg cttcaattag tgcgtcggga gaaattttgt cggtcatacg ggtatccgtg
     2581 ctgaaacggc atgggtttgg atatgccgtc tgaaatcggt tggagtagag agaagctgcc
     2641 tgaaaaatat ttttcagacg gcattcttta tgcttccgaa ggcttctgcc ctttacgctc
     2701 tgcttttctg gcttcgcgta tggctttgag ttcggcttct ttctgacggg cttttttgag
     2761 ccaagtttcc cattggttcg gcgtttcgag ggtgattctg ccgattttgc cttcacggaa
     2821 gtcggtaagg atgttttcgg cggctttttg gtagttgatc cgtccgccgc tgaggactgc
     2881 gccgcgtttt ttggctatcc attcgagcca aacgttttcg tcccagtggc tgctggggtc
     2941 tttgtcggct tggtagcgtt cttgcaacat agggaggtag tggcggcgga ggtagtctaa
     3001 aagttcgagg gcgacttctt cttcgtccaa tgcgttgcgt ccgactgaac cgccggcggc
     3061 gaggttgtag ccgctttctt cgacaatgat tttcggccac agcattccgg gggtgtcgta
     3121 cagccagaag tcgtcggcaa ggaaaaggcg ttgttcggct ttggtgatgc cgggttcgtt
     3181 gccggttttg gcggattttt tgcctatcat gccgttgatg agggtggatt tgccgacgtt
     3241 gggaatgccg caaatcaaga cgcgcaaggg tttgtctatg ccttgccggt ggggaatcat
     3301 cgcgcggcag gcttgggtga ttttgccgtg tgcgccggtt tcggaggaat cgagggcaat
     3361 ggcgcgcgtg tcggggcggc tgttgtagtg gtcaagccag acctttgtgc gttcggggtc
     3421 ggcaagatct tgtttgttta ggattttaag tttgggtttg cctttggaaa gctgggcaag
     3481 cagggggttt tcgctggagg cgggcatacg cgtgtccagc atttcaatca ccatatcaac
     3541 gctttttgca cgctcggcga tggctttttt cgccttgttc atatggccgg gaaaccattg
     3601 gattgccatg tctgttcttt cttttcaata tttgaaatgc cgtctgaaac ggaggacggg
     3661 gtttcagacg gcataatggt ttgacggaat tagcggtctg acaggttttg cgcctgtctg
     3721 tgccgtatca gcaattcata acgccctttc aaccttcggg cgagtttttc gacaatatag
     3781 accgaacggt gctgtcctcc cgtgcaaccg atggcgacgg taacgtagct cctgctttca
     3841 tcctccaaac gcggtaacca atgcgtaaca aacctttcga tgtcgtcaac catttcctgc
     3901 acaagcggct gtccgtccaa ataatcccaa acgggcttgt ccataccggt gtaaggcctc
     3961 aactcgggat cgtaatacgg gttgggcaga ctgcgcatat cgaacataaa atccgcgttg
     4021 ttcggcacac cgtatttgaa cccgaaggac tccaaaatca ccagcagccc ggtacgttcg
     4081 accttcagcc actgccggac tgcatggcgg agctgttggg cattcatctt ggaagtgtcg
     4141 atacaatagg cgatttcttt aagcgggaac agccattccc gttctttctt taagctttcc
     4201 aacaaggtca tatcctgatt actcagaggg tggcctcgcc tggtttcgga aaaccggcgg
     4261 accaacacgc tttcttccgc ctcgacaaac aaaacttcaa ccctgtgccc cagtccgcgc
     4321 agagaggcaa tctgttcccg cgcctgtccg atgtcaatgc cggaacgcac atcgacgctg
     4381 accgccaatt cggtttcgtc cgcacgttcg atatgatacg acaccagcgc gggcaacatt
     4441 tccaaaggca aattgtccac gcagaaataa cccgaatctt ccatttggcg cagtgcgacg
     4501 gatttgcccg aaccggacaa cccgctaatc aggacgattt tcatgattct gttcgttttc
     4561 tttaagttgt gtctgatggc gttccaaaaa ttcgcgcgta ctgtctttgc cacgcaactg
     4621 caaaatgtaa ttgcgtaccg ccgcctcaac caaaacggcg aggttgcgtc cgacggcaac
     4681 aggcagcgtt accgaacgga cgttgacgtt gaggatggat tcggtttcgg tgcggatgct
     4741 caaccggtcg agctgcttca tatactcgtc gtccgcctcg actaaattga taatgagttg
     4801 caggattttt ttggggcgga tggaagtttc gccgaaaata tggcggatat tgagtatccc
     4861 caagccgcgc acttccaaaa aatcgcgcag cataggcgaa caacgccctt ccagcgtttc
     4921 cgggccgatg cggaacagct cgaccgcatc gtcggcaatc aggctgtggc cgcgcgaaat
     4981 cagctccaac gccaattcgc tcttacccaa acccgaatgt ccggtaatca gcacgccgat
     5041 ttcaaacaca tcgagaaata cgccgtgttt gacggacgat gccgccaggg tgcgttgcag
     5101 gtaaatccgc aacacgtcca tcagataggg gctttcgagt ttggaagtca gcagtggaat
     5161 atcgttttta tgacaatagt cgcgcagtcc cggggaaacc ggcaagccgt ttgccacaat
     5221 gaccaaagac atagaaatgt cgaacaggtt tccgaactga taacccgttt cccccgattc
     5281 gaggcggttc agatattccg actccgccaa accaaccact tggatttggt tggggtggat
     5341 aaaattcagg tgtccgacca aggcgaggac gggcttgtcc gcctctacgc cgatacggtt
     5401 gtccgcaccc gaattgccgg cggcccaggc aagttgcagt ttgtattggt tgtcgtcaaa
     5461 cagccggcgg acagagatac tgggcatatt ttattcttcg gtcagaatgg cgcgggcttc
     5521 ttccgcagaa gaaacggtca tcagcgattc tctgatgctt ttttgggaaa acttgccggc
     5581 cagtttggac aatacttcca aatgctcgcc cgtcgcattt tccggaacca gcaagataaa
     5641 aaccagggaa accggtttgc cgtccggtgc gtcaaatccg acgggttcgc gcgtgcggat
     5701 gaacgcgccc gtcgcctgct tcacgccggc atgacggccg tgcgggatgg caacgccctg
     5761 ccccaaaccg gtcgaaccga gtttttcacg ggcaaaaaga cattcgaaaa catcagcatg
     5821 ggacaatgag gattcgcgtt ccaaaagcag gcccgcctcc tcaaacagcc tttttttact
     5881 gcccacctcc atatccaaaa caatatggga caaaggcaaa atttcgccga taaggctcat
     5941 aagcttctct tttcagacat cgcaaaacag aaagattgta ccgactgccg gggcaaatct
     6001 caatcccgca tacggtacgg gctgacataa cacagcgttt taaaaacata ttttaacgct
     6061 tttcggcaca gatagaaatg ccgtccaaag cagtttacgg ctcttcagac ggcattgccc
     6121 tgccttattt cgcgaaaaac gtatgggcaa aaatggcggt aaaccacacc caaccaattc
     6181 tgttgtttgc caaaaacgtt tcaaaacaga tttgccggac gcggcttttg atggcggcat
     6241 attggcggta ttgcagcagc aggacgatgg ggattgccgt ccaatatgcc catgccgcac
     6301 cgataaccgc acccaatact gccatcagca gggtaaagcc tccgtgacac agcataacgg
     6361 cggcgatgtc gtaacgcccg aacgtgacgg cggaggtttt gatgccgatt ttcaaatcgt
     6421 cttctttgtc cgccattgca taaaccgtgt catacgccag agtccataac acattggcgg
     6481 caaagagtat ccacgcttga ggcggcacgt ttccggcaac ggcggcaaac gccatcggga
     6541 taccgaagga aaaggcaagc ccgagataga gttggggaat cggaaaaaaa cgtttggtaa
     6601 atgggtaagt cagcgcaaga aacagggcgg gcaggctcat cagccaagtc agatgattca
     6661 gcggaatcag gcacaatgcg gcaagcaggc acaaaaatgc cgtcagcagc agggcttctt
     6721 ttttcttgac cctgccctgt gcgaacggac ggtttttagt gcgctcgaca gcaccgtcaa
     6781 aatcgcggtc ggcaaaatcg ttgatgacgc aaccggcact gcgcattaaa aacgtaccga
     6841 ttgtaaacgc cgccaatacc gccaaatcgg gaatgccgtc tgaagccagc cacaatgccc
     6901 agtaggtcgg ccacagtaaa agcagcgtcc caatgggctt gtccgccctc atcagtctga
     6961 tataaacgtc caaacgatcg gacaggcgta aaaataaagg ggatttagga ttcatattgc
     7021 cgcacagctt gaaaaaacgg tattttatcc gataaagcgt ttcagttcgg gcagaaaata
     7081 ctcggtcagc agcatttcct cgccgtgacg ggaaaaccga gaacgccgcg cggcaaagta
     7141 ccgtccgcat ccttcgccga aaacggcaaa ctcaaacacc gaacgcgccc cttccaaatc
     7201 ggcttgaaac agacgctcgc ccaaagggcg cgtgccgcag tccaaaatgt tttgccaaaa
     7261 cgccgaaccg atacggcatt cgctccttgc ctcaacaaca gggatgccgt ccagcttcaa
     7321 caaaacttcg cgcaccagcc tccctccgca ttccgtctcc aattcgccca gtttcagcag
     7381 ttccaccgaa aacagatgcg gcaaggcgcg caatgcggcg gtcagcgacc gggtgtgcag
     7441 cagccgcacc atcggcaggc tgatgccgtc tgaaacgtcg gcgggcaggt cgggcagcca
     7501 tttcccaaat aggtgttcca tatttttccc aatctttata ccgcgtctgt tttatccaac
     7561 tccaaccatt ccgcctcggg gaaactgtct ttcagacggc attgcagata gttcaggctg
     7621 tcttgcgcga cacattcgtc ggaactgtcg tgaatatggt tgaaaatcag gctgtgcagg
     7681 cggatgccgt attgtttgag cgcggcgaaa ctgagtaaag tgtggttgat actgcccagc
     7741 cgtccgctgg taacgaggat gacgggataa tcttgttgct gaatataatc aatggttaac
     7801 aaatcttccg tcagcggaac catcaatccg cccgcgcctt cgaccaaaac gacttcgtac
     7861 tgcgccgcca attcttgtgt ggcggtgcgg attttgtcca agtccaaagc cctgccgtca
     7921 agacgggcag cgagatgagg cgaagctgga taactgaaga tttcaggcat ggtcagccct
     7981 tgtttgtcgg cttcctgcat gggtttaccc atgattttac gatggacggc gatgtcgtcg
     8041 gcaatgtttt ggcaaccggt ttgcacgggc ttttgcgtaa tcacgctttt gccctgctgc
     8101 aacaattgtt ttgccaacac gccggtggcg acggttttgc cgatgtccgt gtctatgccg
     8161 ctgacgaagt aaacgccttt catttgctgt gttccttcaa gatttgcacg gttttgtcgg
     8221 caagtttggt caaaacgccg tctgaaatga tgtaaggcgg catcagatac accagcctgc
     8281 cgaacgggcg cacccaaatg ccctgcgcca cgcaatccgc ttgaaaacgc gccatatcca
     8341 cgcctttttc cagctcgatc accccgatgg cacctaaaac gcgcacgtct ttcacgccgc
     8401 gaatgtccca cgcggctttc agacggcctt ttaagatgct ttcaatgcgg cggatatttg
     8461 cctgccagtc ttgagacaaa agcagtttga ccgaagcgca ggcaacggca cacgccagcg
     8521 ggtttgccat aaacgtcggg ccgtgcataa acacgcccgc ttcgccgcgc gaaatcgttt
     8581 cggtaacttt ttgcgaagtg attgctgccg ccagcgtcat atagccgccg ctcaaaccct
     8641 tgccgatgca cataatatcc ggcacgacct ccgcgtgttc gcaggcaaac atcttgcccg
     8701 tgcgcccgaa tccggtggcg atttcatcaa aaatcaaaac gatatcaaat tcgtcgcaca
     8761 aatcgcgcaa tccgcgaaga tactgcggat gataaaaata catgccgccc gcgccctgca
     8821 cgaccggctc taaaataaag gcggcaatat ccgcatgatg cacttcaaat aaggcgcgga
     8881 caggctgcaa atccgcctcg tcccattcat cgtcgaaacg gcttttcgga ttatcgacaa
     8941 aataacgctg cggcaacgcg ctgccgaaaa tatggtgcat ccccgtttcc ggatcgcaga
     9001 cggacatcgc gttccaagta tcgccgtgat acccgcgccg taccgtcgca atattctgct
     9061 tcgccgtcaa cccccgcgcc tgctggtatt gcaccgccat tttcaacgcg acttctaccg
     9121 aaaccgaacc agaatccgca taaaaaatac ggtccaaccc ctgcggcaaa attccgacca
     9181 acaacttgcc cagttccacc gcaggatcgt gcgtcaaacc gccaaacatt acatgcgaca
     9241 tttgcttaat ctgcgcctca accgcctgat tcaaaacagg atgattgtaa ccatgtatcg
     9301 cacaccacca agacgacatc ccgtcaatca gccgtgtgcc gtccgccaat tcgataaaca
     9361 ccccttctgc acgtttgaca ggataaacgg gaagcggatc ggtcatggaa gtatagggat
     9421 gaagcagatg ggtacggtcg aaatcaagca atgatgatgt gtgttgatgt tcagacggca
     9481 taagtttctc tcttttcttc ttactgtatt caaacgcaaa cgcgtattct actccgacag
     9541 accgcttccc acacctttcc atccgtttcg ggcgcaaaac cgcgaaacaa atcgttccgc
     9601 aacataagcg cacaccgttt cgcattcctc aagcccgatt ggaatcagac ggcccaacgc
     9661 ccaataccgt ttcacgcagc ctccgcccaa cgcttcaatc agtcatagat atagtggatt
     9721 aacaaaaatc aggacaagga gacgaagccg cagacagtac aaatagtacg gcaaggcgag
     9781 gtaacgccgt actggtttaa atttaatcca ctataaaacg gcaatccata cgatacagat
     9841 catagcaaca gccatcgcaa cagcgttagc aaaatcaggg gactccgaca taggcgcata
     9901 gcacctaccg atgcacggct cctcattcgg ctctatgaat accataccca tcacaaaatc
     9961 caccgccaaa accaggcacg gcttcttata cttatgatag atttccacca tcctgctcca
    10021 tatatcccaa acattcatac cgtatatccc gcaggcaaca aattccgatt gaaggttaca
    10081 gcccta
//
LOCUS       DAXIEB010000075         9879 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000075 DAXIEB010000000
VERSION     DAXIEB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.075"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            43..198
                     /locus_tag="ACFOA3_001476"
     CDS             43..198
                     /locus_tag="ACFOA3_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898467.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAE
                     EPPYQAS"
     gene            207..1256
                     /locus_tag="ACFOA3_001477"
     CDS             207..1256
                     /locus_tag="ACFOA3_001477"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6898468.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(1325..2866)
                     /locus_tag="ACFOA3_001478"
     CDS             complement(1325..2866)
                     /locus_tag="ACFOA3_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6898469.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(3477..4832)
                     /gene="xseA"
                     /locus_tag="ACFOA3_001479"
     CDS             complement(3477..4832)
                     /gene="xseA"
                     /locus_tag="ACFOA3_001479"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6898470.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            5029..5850
                     /gene="nadE"
                     /locus_tag="ACFOA3_001480"
     CDS             5029..5850
                     /gene="nadE"
                     /locus_tag="ACFOA3_001480"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6898471.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGMYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(5920..6393)
                     /gene="dnrN"
                     /locus_tag="ACFOA3_001481"
     CDS             complement(5920..6393)
                     /gene="dnrN"
                     /locus_tag="ACFOA3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6898472.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            6691..7023
                     /gene="trxA"
                     /locus_tag="ACFOA3_001482"
     CDS             6691..7023
                     /gene="trxA"
                     /locus_tag="ACFOA3_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6898473.1"
                     /translation="MSSELIVHTGDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(7076..8023)
                     /locus_tag="ACFOA3_001483"
     CDS             complement(7076..8023)
                     /locus_tag="ACFOA3_001483"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6898474.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            8351..9739
                     /locus_tag="ACFOA3_001484"
     CDS             8351..9739
                     /locus_tag="ACFOA3_001484"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6898475.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
BASE COUNT         2416 a         2729 c         2593 g         2141 t
ORIGIN      
        1 atttgaaaga ttctaccccc gcacgcaaac cgatttcaac cgttgcacaa actttgcctc
       61 gccacaagcc gcagcgacga tttcatccgc aaaaccgccg catcaggtac aatatcgaac
      121 cgtccgactg aggacggcat tttatcaacc cgtcctgccg cacacgccgc agaagaaccg
      181 ccttatcagg cgagttagga aaaatgatgt ccaaacagcc caccagcaaa cgccaatggc
      241 gcgacggcgc agccccgtct gccaagaaaa ccgccaaacc gttcaaaagc aaagcccgtc
      301 ccaaagacga aacggacaaa accgcatccc aaccttacgg acaaaaagct tcagacggca
      361 tcaaacctca aaacctcccc aaacagcgcg ccgccaaagc caaaaaactc gtcgtccgca
      421 atcccaacca aaaaattatg gaacacgcgc gcgatttaaa agaacgccgc agcgacctat
      481 cgcgcatgga acccgaacgc ctgcaaaaag tgcttgccgc gtccggcgtc ggctcgcgcc
      541 gcgaaatgga agaatggatc aacaacggct gggtaacggt caacggcaaa accgctcaac
      601 tgggcgacaa agtcaccccc gacgaccacg ttaccgtcaa aggcagcatc atcaaactca
      661 aatgggccga ccgcctgccg cgcatcatcc tatattacaa acaagaaggc gaaatcgttt
      721 cccgcgacga cccgcaaggc cgcgtcagca tattcgaccg cctgccgcag gccgccagca
      781 gccgctgggt cgccatcgga cgcttggaca tcaacaccag cggacttctg attctcacca
      841 cctccggcga actcgtccaa cgtttcgccc accccagctt cgaagtcgaa cgcgaatacg
      901 ccgtgcgcgt actgggcggg ctgaccggcg aacaaatgcg cgtcctcacc gaagaaggcg
      961 tgatgctcga agacggcttg gcaaaagtcg aacgcatccg cgaacaaggc ggcgaaggcg
     1021 cgaacaaatg gtacaacgtc gtgattaaag aaggccgcaa ccgcgaagtg cgccgcattt
     1081 ttgaaagcca aggactcacc gtcagccgcc tcgtgcgcat cggcttcggt cccatcggac
     1141 tgcccaaccg cctcaaacgc ggacagttct acgaactcaa ccccgccgaa gtcgccaaca
     1201 tcatcaaatg ggcggacatg ctgctgccgg gcgaacgccg ccgcaaaaaa gcctaaaccc
     1261 gccaaaacac aaaaatgccg tctgaaacat ctgctgtttc agacggcatt ttattcgggc
     1321 gttttcagga gaaaaggtcg agtgctttga caaagaccat caccacgccg taggcgagcg
     1381 gtatgacggc caatgcccaa cgccaccaga ccgatatgcc gccgccggaa actttttcgc
     1441 ccacaaggta agcgtcggat atggcggttt cgtcgtcggg gttgccgctg tgtgcggcgg
     1501 ttttgatgtc tttttcgtgg tgtttttcgt gtacggattt gacggcgagg ttgcacaaca
     1561 aaccgataat cagcaggcac gccatgatgt acatggttac gctgtatgcc tgtgccgccg
     1621 gtatgccgct gtcgatttgg ctttggcgta tgtaattgac cagtaccggg ccgatgacgg
     1681 cggcggttga ccaggccagc aggatgcgtc cgtgaatcgc gccgacctga taggtgccga
     1741 acaggtcttt caggtaggcg ggaatggcgg caaatccgcc gccgtacatg gaaataatca
     1801 cgcaaaagcc gatgatgaac agggctttgc tgccgccctc gccgatggag ggaacggcga
     1861 aatacagcag cgagccgagt acgaagaaga tggtgtaggt gtttttgcgt ccgattttgt
     1921 cggaaacgct cgaccacaaa aagcgtccgc ccatgttaaa caggctcagg aggctgacga
     1981 agcctgccgc cgcacctgcg ccgactgctg cctgcctgcc tatggaggtt tcggaaaaga
     2041 gttcctgaat catcacggat gcctgaccca atacgccgat gccggcggtt acgttcaggc
     2101 acaataccca gaacaacagc caaaactgcg gcgttttcat ggcttgggac acgttgacat
     2161 gattactgct gaccagcttg ttttgcgttt tcggcgcggt atagccttca ggtttccagc
     2221 cgtcggcagg tacgcggatg gtaaacgcgc cgaacatcat cagtgcgagg taaagcaggc
     2281 ccaatacggc aaaggtttcg gcaaccccga ccgaagcggc gtttgaaaag gcgttcatca
     2341 gtgatacgga aagcggagag gccagcattg cgccgccgcc gaaacccata atcgccaaac
     2401 cggtcgccat acccggcttg tcgggaaacc atttcatcag tgtggaaacc ggcccgatgt
     2461 agcctaagcc taagcctacg ccgccgatga cgccgttgcc caaatagagc aggaagaggt
     2521 tgtgcgtacg cacgccgaat gcggatacga agaagcccag gctgaaacag caggcggcgg
     2581 caaatatggc tttgcgcgga cctacccgtt ccatccatgt gccgaacagg gcagccgacg
     2641 cgcccagcat cgcgagtgcg atactaaaaa tccaacctac ggtcgtcagc ttccaatctc
     2701 cggccgccga ttcggttatg ccgataagtt tggtcagcgg cgcgttgaat acggaatagg
     2761 cgtaaatctg cccgatggca aggtgcaccg ccaatgctgc gggcggtacg agccaacggt
     2821 tgaaacccgg cttggcaatg cttgcctcac ggtctaaaaa cttcataaca tcctctttct
     2881 gtcagttgaa aaataaaatt tcatttgctc aatggaaact tattgaaaat tataaaaaaa
     2941 tatcgggtca ggtttttatc cgccccaaga tgcgccgtct gaaacatttc gggtgtacgg
     3001 aaaggtttct gttttttccg acaaattcct gcggcttttc gcttccggat tcccgctttt
     3061 gcgggaatga cgaattaaag attatcttaa ggtcaaggga ctggattccc gcttttgcgg
     3121 gaatgacggc ggcgggttgc tgttttttat ttataaattt ctgtaacttt ggatttcagc
     3181 catcttcaat aaattatcga aacgtatcaa tcgtcattcc cgcgcaggcg ggaatccatc
     3241 cagttcgttc gatttcggtt ttttaagttt cgagtaactt ctgactcgtc attcccgcgt
     3301 aggcgggaat ccggtttttt gagtttcagt catttctaat acattcccac cacttttcgc
     3361 ttccggactc ccgctttttc aggaatgacg aattaaagat tatcttaagg tcaagggact
     3421 ggactcccgc ttttgcggga atgacggcgg agggggagcg gtttttccga ttgggtttaa
     3481 atgcaatcga acaaatcctg ctgcccttgt tctttgctta cgcgcacgtc ggtttcgccg
     3541 tcggcgaagg taatgtgcag cttctgcccc tgcttcaaaa catcggcgtt gcggatgact
     3601 tgtccgcgtg tgtttttgac gacggaaaag ccgcgctcca gaatgtgctg cggcgaaacg
     3661 gcttcgagca atgcggcttg ggcggtcagg ctttggcggc ggtgggtaag cagttggctg
     3721 aaggcggcgg gcaatgccgt ctgaaggcgg acgatgttgt ttttgcaaac ggaaacatca
     3781 ggacggtaat gttgcagggc ttgggtttgg cgttcaaaac gggcggtgtg ggcacggagg
     3841 ttttgcttca tagagtaaga cagcgtttgc gccagcttgc cgattgaagc gcgctgctca
     3901 tcaagttttt ggcgcggatg acggatttgc cgcgcaagcc agtcgagttt ttggctggca
     3961 tcgaaatagc gttgttccaa aacggttttc agacggcctt gcgcctgagc gagacgatgt
     4021 agcgattctt ggcggttggg gctgaccagc tccgccgcac cggtcggcgt gggcgcgcgc
     4081 acgtcggcga caaaatcggc aagcgtaaaa tcggtttcgt gtcccacgcc actgacgacg
     4141 ggaaccgtgc aggcttcgat tgcgcgcacg accggttctt cgttaaacgc ccacaagtct
     4201 tcaatgctgc cgccgccgcg acagacaatc aacacgtcgc attcggcgcg ttgcgaggcg
     4261 gttttaatcg cttgggcaat ttgtaactcg ctgcctgcgc cttgaacagg tgtcggataa
     4321 acgataacgg gaatttcggg cgcgcggcgt ttcaaggtgg taacgacatc gcgcaatgcc
     4381 gctgctgcca ggctggtgac gatgccgata cattgcggac gggtaggcaa cggctttttg
     4441 cgttccgccg caaacgcgcc ttccgcctgc aactgtgcct tcaaccgctc gtaggcttcg
     4501 taaagctgcc ccaaaccttt gagccgcacc tcattcacgg taatttgaaa ttcgccccgc
     4561 gcctcgtaaa tactgatttt tcctgatact tcgatatggt cgccttcttt caaaggcttc
     4621 gccaaacgcg ccgccgcacc tttgaacatc gcgcaacgca cctgcgcgcg gctgtctttg
     4681 agcgagaaat aataatgtcc gctggcggca cgggtcaggt tggacacttc gcccgcaatc
     4741 cacaggccgg caaggtggtt ttccaaaaga cttttggcaa atgcgttcaa ctcggaaacg
     4801 gacaacacgt cagaatgaaa aaaatcagac atagaatcaa tcaaataata aaaaatatga
     4861 atatgttttg aagcctaagg cggtattagt ccgcctaaat tgtcaacaat attataacac
     4921 gcgccatctt gccgcccgcc ttttcccgta tgactttttt aagcggggaa tgggaaaaat
     4981 attcatcaac ccgccctcaa tctattcaaa ttacaccgcc gacaagctat gatgcggata
     5041 ttttcgacag gaggaaaaat ggatacgcag gcagttatca cacatattgt ccgatggttg
     5101 gacgaatacg ccgcccgggc aaatgcaaaa gggtttgtcg tgggcgtttc cggcggcatc
     5161 gattccgccg tcgtctccgc actcgccgcc cgcaccggcc gcaccacgct gcttctggat
     5221 atgccgatac gccaacaccc cgaccagctt gagcgggcaa ggctgcacat ccgcaatctg
     5281 caacggcaat atgccaatgt aagcgcacaa acggtcgatc tgaccgacac cttccagacc
     5341 tttgaacaaa ccgtcggcgt tcatcagacg gcatttgaca atcagccgct ttccctcgcc
     5401 aacgccagaa gccgcctgcg tatgctgacc ctgtactact acgggcagat acacggacta
     5461 ctggttacgg ggacaggtaa caaaattgaa gatttcggtg taggattttt taccaaatac
     5521 ggcgacggcg gcgtggacat cagcccgatt gccgacctga ccaaaaccca ggtttaccgg
     5581 cttgccgaag cattgggcgt ggacgaggcg attcaaaaag ccccgccgac cgacggcctg
     5641 tgggatacgg aacgcaccga cgaggaacag atgggcgcga gctatcccga actggagtgg
     5701 gcaatgggca tgtacggcac gcgcaaaccc gaagattttg aagggcggca gcgcgaagtt
     5761 ctggaaatct atacgcggct gcaccgcgcg atgcagcaca aaatcaaccc gattcccgtg
     5821 tgccgcattc cgcccgaatt gttgggctga aacgtggaaa tgccgtctga aacggaaaac
     5881 cgtatttcag acggcatgga aatatccgac tcctatccct taagaatcga gtacgcgggc
     5941 aaacaaaata tcgttttcca aatgaatgtg gtcgttcaaa tcctccacca tttctttcgc
     6001 cagcgcgtaa agccgcgtcc agcttccgca agccccttct ggcggttgga aattgtcggt
     6061 cagctctttg agccgtgcga tggcgcggtc gtgttcttca tgttcgtgca tcatcacgcc
     6121 gatgggcatc gccgcaccgc gtccgacacc ctgattaatc atcggaaaca gcatccgctc
     6181 ctccttcatc atatgcatca gcagttcgtt ctgcatatag gcaagcagcc cggcaatttc
     6241 tgccggaaag gtatcggcat ggacttgggc cactttctgc gccagcggca ccaattcttc
     6301 aaattgtgcg cggtggacat tgtggtagcg ttgcaggata tgatcgacgg ttgcaccaaa
     6361 gggggcggtc tcccaaacgg aaaaatcagt catcgcagtg ttccttttac agggtttcgg
     6421 gtttggtttt gaacattcat actttaagaa tcaattcaaa cggagcatac accgcccgcg
     6481 cgcttctgta cagcctcaaa cgtattcctt acattttgat aataaaagta attttcagaa
     6541 ataaaatact gtccgaaccg ttttttagaa tttgcaaagg cgattggggc ggtacagaaa
     6601 aactattatc ccgcccgccc acttgaaatt tttatgccca agccctatcc tgcacgctat
     6661 cgtgccaatc ccaaccgaaa aggaaaaata atgagcagcg aattgattgt acacaccggc
     6721 gatgccgctt ttgaaaaaga cgttttaaat gcagatatcc ccgtcctgct ggacttttgg
     6781 gctccgtggt gcggcccctg caaaatgatt gccccgattt tggacgacat tgccgccgaa
     6841 tttgaaggcc gtctgaaagt ggtcaaaatc aacatcgacg acaacgaagc caccccgtcc
     6901 cgtttcggcg tgcgcggcat tccgaccctg atggtgttca aaaacggcga agtcgtcgcc
     6961 accaaagtcg gcgcattggc aaaaggtcag ctgaccgcct ttgtcgaagc ctctatcgcc
     7021 tgataaagcg caatcgaaaa agccgccgga agattccggc ggctttttcg cacccttaag
     7081 atttgtggcg gatttcccag cacctatgga tttttttgtt gcggaaatct tcgggaacgg
     7141 attgtttgga aatgtctttg acggcgtatt gttccgatac caagtcgtct aagacgaagc
     7201 tgcgcaggtt gttggaaaag tacaaaatgc cgtctgaagc gagcagcttc accgcgccgt
     7261 caatcagctt tttgtggtcg cgctggatgt cgaggatgtc ggacattttc ttgctgttgg
     7321 aaaaactggg cgggtccatc acaatgaggt cgaaccgcct gccttcccca tatgccgtct
     7381 gaagatattg gaacacgtcg gcgcggacga ttttatgccg ttccgtatcg atgccgttca
     7441 attcaaaatt gcgtctcgcc caatcgagat aagtgttaga caaatcgaca gtttcgctgg
     7501 atgccgcgcc gccggtggcg gcatagacag tgaagctgcc ggtgtaggaa aacaggttta
     7561 aaaaacgttt gcccgccgcc gtttcgccga cttttttgcg cgtgtttcga tgatccaaaa
     7621 aaagccccgt atccaaatac ttatcaaggt tgacccaaaa cttgcggccg ttttcggtga
     7681 tgacgaaatc gtcgcccgcc ttgccggttt tttcgtattg ctgcaaacct ttttggcgtt
     7741 cgcggcgttt gaggcggatt tgttcgggcg caaaaccggt aacgaaggcg acggcttcca
     7801 agacttcggc aagccacgct tcgtattctt cgggccgcat cagccagccg gtatcgtatt
     7861 cctgaaggtg gattcgatcg ccgtaaacat cggcggcaaa ggggaattgg gggatgtcgc
     7921 ggtcgtaaat gcgccaggct tcgatgccgt tgcgtttcgc ccatttcata aggtgtttga
     7981 tgtttttgcc caagcggttg gcaaacggtg tgatgtcggt cattggtttc aggcggaata
     8041 aagtggaaaa cggcaatttt actgtaatta acgcccgatt gcttgaccgt ttcgggcaaa
     8101 ccctatacca tccaccgctt atcttgtcat acgaagccat cgccttccaa cctaaaccgc
     8161 ccttacgggc gcgtttcttc tgttgctttg attttgcaaa gcgtatctgt gcaggttgcc
     8221 gtcgatgtaa accacaagca agccgcttgc gacaaccctg taacttcaca ttccccgtat
     8281 cgttaccctt ccctgcttca ggccgtctga acctttcgga cgcgggcgtt gttgtcttcc
     8341 aaggatagcc atgtctatta aatttgccga tttgaacctt gataaaaaca ttttgtccgc
     8401 cgtcagcagc gagggttacg aaagcccgac gccgattcag gcgcaggcga ttccgtttgc
     8461 tttggaaggc cgcgacatca tggcttcggc gcaaaccggc tccggcaaaa ccgccgcctt
     8521 tctgttaccg actttgcagc gactgaccaa acgcagcgaa aaaccgggca aaggcccacg
     8581 tgctttggtg ttgaccccga cccgcgaatt ggcggctcaa gtcgagaaaa acgcgctggc
     8641 gtatgccaaa aatatgcgtt ggttccgtac cgtcagcatc gtcggcggcg catcgttcgg
     8701 ctaccaaacc cgcgccctga gcaaaccggt cgatctgatt gtcgccacgc cgggccgcct
     8761 gatggacttg atgcaaagcg gcaaagtcga ttttgaacgt ttggaagtgc tgattctgga
     8821 cgaagccgac cgtatgttgg atatgggctt tatcgacgac atcgaaacca tcgtggaagc
     8881 aacgccgagc gaccgtcaga ctttattgtt ctccgccact tgggacggcg cggtcggcaa
     8941 actggcacgc aaactgacca aagaccctga aatcatcgaa gtcgaacgcg tggacgacca
     9001 aggcaaaatc gaagaacaac tgctgtactg cgacgatatg cgccacaaaa accgcctgct
     9061 cgaccacatc ctgcgtgatg ccaacatcga ccaatgcgtg attttcacgt ccaccaaagc
     9121 catgaccgaa gtcatcgcgg atgaattgta cgaaaaaggt ttcgccgcca actgcctgca
     9181 cggcgatatg ccgcaaggct ggcgtaaccg cacgctgatg gacttgcgca aaggccgctg
     9241 caaaattttg gttgccaccg atgtagccgc acgcggtatc gacgtaccga ccattaccca
     9301 cgttatcaac tacgacctgc cgaaacaggc ggaagactac gtccaccgca tcgggcgcac
     9361 cggccgcgca ggccgcacgg gtattgcgat tacgtttgcc gaagtgaacg aatacgtcaa
     9421 agtccacaaa atcgaaaaat acattaaccg aaaactgccc gaactgacca tcgagggcat
     9481 ggaaccgacc cgcaaacgca aatccgcagg cggcaagccg aaaggcaaag gcggctgggg
     9541 cgatcgtaaa tccggcggtt ggcgcggcga tcataaaccg ggcaaagaag gcttcggcgg
     9601 caaaacgcgc ggcgaaggtt tcaagaaaga aggctttaag aaagacggtt tcaaaaaaac
     9661 cggcgaaggc ttcaaaggca aacgtaaagc cggcgattct tttgcaggca aaagcgaacg
     9721 ccgttacaaa gaccgctaag ccccaacctg cctcataaac caatgccgtc tgaaaccgat
     9781 ttcgagtttc agacggcatt tttgcaatgt ttcaacaccg cccggctttg atacccaaag
     9841 gattaatctg caatatagtg gattaacaaa aaccagtac
//
LOCUS       DAXIEB010000076         9598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000076 DAXIEB010000000
VERSION     DAXIEB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.076"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            249..563
                     /locus_tag="ACFOA3_001485"
     CDS             249..563
                     /locus_tag="ACFOA3_001485"
                     /inference="COORDINATES: protein motif:HMM:NF037917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898476.1"
                     /translation="MQELAGMTRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQ
                     NPEHVIRDNRELIFTARYKGSALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKV
                     LK"
     gene            673..1089
                     /locus_tag="ACFOA3_001486"
     CDS             673..1089
                     /locus_tag="ACFOA3_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6898477.1"
                     /translation="MIDVKIDNIFVVLNQIEQLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            1320..2381
                     /locus_tag="ACFOA3_001487"
     CDS             1320..2381
                     /locus_tag="ACFOA3_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6898478.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            2443..2769
                     /locus_tag="ACFOA3_001488"
                     /pseudo
     CDS             2443..2769
                     /locus_tag="ACFOA3_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2757..3454
                     /locus_tag="ACFOA3_001489"
                     /pseudo
     CDS             2757..3454
                     /locus_tag="ACFOA3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            3451..4011
                     /locus_tag="ACFOA3_001490"
     CDS             3451..4011
                     /locus_tag="ACFOA3_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6898479.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAMAESAQSV
                     ADAVDPRSAGQMLADWECVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            4022..5995
                     /locus_tag="ACFOA3_001491"
     CDS             4022..5995
                     /locus_tag="ACFOA3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6898480.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPIENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQVSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDRNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGARRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(6652..6954)
                     /locus_tag="ACFOA3_001492"
     CDS             complement(6652..6954)
                     /locus_tag="ACFOA3_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6898481.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(6926..7213)
                     /locus_tag="ACFOA3_001493"
     CDS             complement(6926..7213)
                     /locus_tag="ACFOA3_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6898482.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            7293..7898
                     /locus_tag="ACFOA3_001494"
     CDS             7293..7898
                     /locus_tag="ACFOA3_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898483.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            7877..8173
                     /locus_tag="ACFOA3_001495"
     CDS             7877..8173
                     /locus_tag="ACFOA3_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6898484.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            8170..8379
                     /locus_tag="ACFOA3_001496"
     CDS             8170..8379
                     /locus_tag="ACFOA3_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898485.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8550..9598)
                     /locus_tag="ACFOA3_001497"
                     /pseudo
     CDS             complement(8550..9598)
                     /locus_tag="ACFOA3_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
BASE COUNT         2429 a         2706 c         2631 g         1832 t
ORIGIN      
        1 ctgaaggcga tgtgtttgaa cctgttgaaa gccgccaaca ggctaagtgc gcccgctgcc
       61 gcctaaaaag cgaccggatg cctgattatc gggtatccag ggaagattaa gggggtattt
      121 gggtaaaatc aggaggtatt tggggcgaaa acagccgaaa acctgtgttt gggtttcggc
      181 tgtcgggagg gaaaggaatt ttgcaaaggt ctcagcttaa atttgctgcg ggtgtattga
      241 gcaaggaaat gcaagaactg gcaggtatga ctcgagcgac ggtgtggctg tctgatgata
      301 cattggtcaa acaggtggac agtcgggagg ggcagaattt cgatgactcc tactatgctt
      361 ttttgccgga tatgctgcaa aaccctgaac atgtcatccg cgacaatcgt gaattgattt
      421 tcacagctcg ctataaaggc tcggcattgt gggcagtttt aaaatatatt aaggaggtgg
      481 atgagattta tctacagtcg taccgaatca gtaacgacaa agagattgcc aaatttatgg
      541 cgaagaagaa agtattgaaa tagacgttgg gcaaggctcg aattcacttg cacacgctct
      601 cggacaccct aaagggcagg ctgcggtatc gagattatca ccgcttttcc aacgcctata
      661 aggaatcata ccatgattga tgtcaaaata gacaatatct ttgtcgtcct aaaccaaatc
      721 gagcagcttg gcaacgggat cgaaaaccgc tacctgctga tgcgccgact gtccgaaacc
      781 atgcacacgg cggtcaagct caatttccgc tacgcaggcc gtccgaaatg gttggggcta
      841 aaataccgcg acggcaagcc gctttcggat tcgggtcgtc tgaaagacag tttttccaca
      901 ctgtcagaca acgataccgc ccttgtcggt acgaatatcg tctatgccgc catccacaac
      961 ttcggcggta tggcggggcg caaccgcaaa gttcggattc cgcaacggga atttttgacg
     1021 ctgacggacg acgacaaaca ggctttgatg gacgatgtgc aggattattt ttcgggtctg
     1081 ataccgtgaa tttataaaac cctcaaaaac gcgcttttta gcgcgttttt ttatgcgggt
     1141 aatacaaacc cctgcccaag atataaaaat caatcctaga cgcttctaaa aagcccctga
     1201 aaacgattaa ttgtgtatcg cgcggacagg ttttaaaaaa atggcgggag ggtttgaagc
     1261 acgcctactc tttgttgttt tttcaaatag gcaaaatgac cgtattgaga gaggtacaca
     1321 tgtccaaaaa tgcacaaaaa accctacttg ccgtgtgcag tttcgaggtg cagccaaaag
     1381 acgggcgaat ccaactgctg ccatatggcg aatttcgcgc agtagacggt cgtccgactg
     1441 atgtccctgc gtggtatctg accgaagaaa acggtcatga tgtcgcgttg ttggccaaca
     1501 gctcgcgcaa tcagttggtt gtcgattatg aacaccagac gctctacaaa gagaaaaacg
     1561 gacaacctgc acctgccgcc ggttggatgc gttggctgga gttcacgcct aaaggcatgt
     1621 ttgccgaagt ggagtggacg gacaaggcgg ctgcggcaat tgccgcaaaa gagtatcgct
     1681 acatctctgc tgtgttttcc tatgacacaa agggatatgt aagcaaaatt tttcacgcag
     1741 cgctgacaaa tttccccgcg ttggacggta tggacgaggt gctggcggca gcgtcggcgc
     1801 aaattttaaa accggaaacg gagcaaaacc ctatgaaaga gttgttacag caactgttcg
     1861 gtctgcctga tgcgggcgaa gaagaactga aggcggcatt gtccgcgctc gtggaagcca
     1921 agccgaaaga cgtggcattg tctgccgacg tgttcgcgca gctggcggaa aaagacagcc
     1981 gcatcgcggc attgacggcg caaaccgcca agcctgattt gactaaatac gcgcctatct
     2041 cagtggttca agagctgcaa agcaaagtcg ccgcgctgac tgccaagcag gaagcagaca
     2101 aaggcaacga attgattacc gccgcgctga cttcaggcaa attgctgcct gctcagaagg
     2161 agtgggcaga aggcgtattg aaacagccgg gcggcttggc atttttgacc ggctttattg
     2221 aaaacgccca gccggtcgct gcactggcag gctcgcaaac gggcggcaaa gcacccgacg
     2281 aacgcgtcgc cgcactgact gcggaagagg cagccgcagc aaaaatgctg ggcatgtccg
     2341 gcgaagaatt tgtaaaaatc aaagaaagcg aaggtaagta atggacaaat cagcgatttt
     2401 gaccgcaatc acggcagcat tccgcaaaga atttcaaacg ggggcctgca aaaccgcctg
     2461 aaccgtttaa ccgccaaaca ggtgcagccg gtagcgcagg cagtgcgcct gttatccacg
     2521 tcatcgctgt tgtcggtggc aacggcatta atcgaggcgc atggcgaaga gatgaccgcg
     2581 cccgatttga ttgaggttaa ccgcgccatg cgccgccgta tgcaggccga gattgccgcc
     2641 ttgcgggcgg tgcagacggc tgctgccgag tctggtgggc tgacggccaa cgccgtgtat
     2701 accgaggctt accaaacggc agaatccctg cgcgcggcgg caggccgtct gaatgcgttg
     2761 gttgcggcgg cacggcggaa ataccggcaa tcgccgacga gccgggcgcg gccgccaatg
     2821 tggcgacggc gaggcgcaac tgatggccgc ccccgccggt gtggccaccg aatgccgcct
     2881 taccgtacaa ggcggcaccg accgagaaag cgatgcctca ctgctggcgc gtctgttgga
     2941 aatcatccgc cgaccgcccg caggcggcaa ccgttacgac tataaaaact gggcgttgag
     3001 tgttgacggc gtaaccagcg catatgttta tccgctgcgc cgcggcttgg gtacggtgga
     3061 tattgccatt acctccgccg acggtgtgtc gtcggaagaa actgtgcgcc gcgtacaggc
     3121 ttatatcgac gagatgcgcc cggtaacggc aaaaaatgcg ctggtactca agccaaccgt
     3181 aacggcggtg cctgttaccg tgcaagtcaa gctcgacggc atcgacttgg acgaggccaa
     3241 gcgccgcata cggacggccc taaaagaata tttcgacacc ctgatccccg gcgacggcct
     3301 gactgtgtcg caaatcgagg ctgctatcag caatgtggat ggtgtgatcg accgccgtct
     3361 gactgcgccg acggccaacc gtgccgccga tacggttaac cgcatcgagt ggtttaaagc
     3421 gggcgcgatt aatgtaacgg agatgccgtc atgagctatc aagacatctt gcggggcctg
     3481 ttgccccccg tgtcgtatgc ccgcaatgcc ccgcgtgtgc gggcgcaggc agaaatagac
     3541 ggcgcagcgc tggatgcgat ggcggaatcg gctcaaagcg ttgccgatgc cgtcgacccg
     3601 cgcagcgccg gccaaatgct ggccgattgg gagtgcgtat taggtttgga cggtacgggc
     3661 aaaaaccgcc agcgccgtgt gttggccgtc atggccaagc taaacgaaac aggcggcttg
     3721 agtattcctt attttgtgcg tttggccgag gcggcgggct atcaaatcca aatcgacgaa
     3781 ccgcagccgt tccgcgccgg tgtcaaccgt gcgggcgacc gtcttgcgcc gcaggaaatc
     3841 atgtgggtgt ggcacgttaa cgtgcgcggc ggcaacaacc gcattacccg attccgcgca
     3901 ggtatctcgg cggcgggcga caggctgacc gattacagcg atgccgtgat cgaaagcctg
     3961 ttcaaccgcc tcaagcccgc ccacaccgct atccgattta cctaccgata aggactaaac
     4021 catgcacccc atcgaaaccc ccgacaagac cttccacgac ggcgacggcg tgtccgaatt
     4081 gggcaccatc ctgcccgcgt ggtggctcaa ccaagtgcaa tccgagctgc tggccgtgct
     4141 gactgcggcc ggtatccagc cggataagtc gcagcctaat caattactgg cagcactaaa
     4201 taggctggct gtagttatca ccggcgacca aaccatcacc ggccaaaaaa cctttaccgc
     4261 ccaaacccag ttccaaagcg gcatccattt atccgccaac caaacgaact ggaacggcgg
     4321 ccacaaagcc tacatcggcg cggatgccga caacgcccac atcgtcttcg gcgacgacac
     4381 cctccgcctg cacggcgcaa acaaccgcat ttcctacaac aaccacgaca tcttccacaa
     4441 agccaacaaa ccgcgttttg ccgaagacat cgaaggcaag ccgaacacac tgtccggata
     4501 cggcatcggc aatttcaaag tcgaaacatt ccggggcgat ttgaacaccc tcaaaacaga
     4561 cggcatctat tccctgccga cggcggtcgg cagctccaac ctgcccattg aaaacaccgc
     4621 ctgccatatc caagtcatcg ccggcacgca acccggctgg tgcaggcagt tgggttatcc
     4681 cgcctacacg tccgacgtgt acgaacgcca ccaggtaagc agcgcaaacg acaactggtc
     4741 cgcatggaaa aaactcaatt cggacggcat ccccgtcggc gcgatcgtat cctttcccaa
     4801 agccgtacaa aaccccgcag gctatctcaa agccaacggc acgacctttg cacaaaacac
     4861 cttccccgac ctctaccgcg ccctgggcaa cagcaaccgc ctgcccgatt taagccgtac
     4921 cgacatcggc atcaccgcgt ggtttccgtc cgaccaaatc ccgaccggct ggctggcgtt
     4981 tgacgacatc cgcacgcgcg taaccgaaac cgcttatccc gagctgtacc gtctgctgac
     5041 cggaaaatac ggcagcatcc aaaacgtccc gcaggcggaa gaccgcttta tccgcaacgc
     5101 gggcaacagc ttggcagtcg gaacgaagca ggaagacgaa atcaaacggc acgtccacaa
     5161 agtattttca cactggacaa accacacaga cacggcagcc gtcggttacg aagaccgcaa
     5221 cgaaaggcag agaagcgcgc tcgtatcgac ttggacggac gaaaatttaa acgacaacgg
     5281 ctttttaacc ccgcgcccgg acagcaaaat ggcgacaggc ggcgacgaaa accgccccaa
     5341 agccttggtt ttaaaactgt gcatcaaagc cgcagacacc ttgggcgaag ccgtgttctg
     5401 gataaagtcc cacggcgaaa ccatcaacgc aggcgcgctg gacgcgggca cgctggcgca
     5461 aggtttgcaa gacaaagccg accgcgacca cacccacacc gccgcccaaa tccaagggct
     5521 ggacgaaaaa atcagcaccg ccgttgccgc gcaattcaca cgccaaacca tcggcggcgt
     5581 ggatattgtc agattccccg acggcacaat gatacagacc ggcagctaca ggttcacacg
     5641 aagcggcggc cccatcgaaa acgaagtcgt cttccccgtc gcctttgccg acggcaacgt
     5701 caaatgcttc gtatccgaac gccattcggg acgcgttacc ggcgctcgaa ggcaacacaa
     5761 ctggctgttt atccgcgcaa aaaaccacgc cgccgccatt attaccaact ggtacgaagg
     5821 cagttgcgac tggatggcca tcggcaaagc cgcctcggga aacgccgcca gctccccgat
     5881 aggccccgaa atacctgaaa ccaacgaaga accgcaaaga gagagtggaa gaacatcaac
     5941 cggaccccga aaccgccgcc gccgagacgg cttgctcgag gcactgcaag actagcgggc
     6001 tgtagagatg gctgtagaga tggctgtaga gacgggctgt agagatggct gtagagacgg
     6061 gctgtagaga tgggctgtag agatgggctg tagagatggg cttcagcccg ccgatccaag
     6121 caatccgacc gaaaccggcc gcgccgccaa tcccccgaaa cctatgcccc gccaatcctg
     6181 ccactcttcg tcattcccgc cgctttcgtc attcccgcga aagcgggaat ccagccccca
     6241 aacgcggcag gaatctatcg gaaaaaacag aaacccccgc caccgtcatt cccgcgcagg
     6301 cgggaatcca gaccccgacg caacaggaat ctatcggaaa aaaacagaaa cccccgccgc
     6361 cgtcattccc gcgaaagcgg gaatccagac cccaaacgcg gcaggaatct atcggaaacg
     6421 gctgaaaccg aacggactgg attcccgcct gcgcgggaat gacggcggca ggggtttcgg
     6481 gattcccgcc ttcgcgggaa tgacggaaag tggcgggaat aacgaaaggc gggaatgacc
     6541 gcgcaaaaag ccgctgcccc cttcggacgg caccggcaac aaaaaaccgc acggccgaaa
     6601 ccgcgcggga aaggatagtc gggcgcgccc gataagcagc ggccgccccc gttatttcaa
     6661 ttgggcgaca tattggcgca aaacctcgtt gatgcgcgtc tgccagccct tgccgccggc
     6721 gcggaatttt tcgaccacat cggcggacag gcgtatggta acgagttgtt tcggggtttt
     6781 gcctgtgttt cgtttttgca ttacgccctt ttcttccaat tgtttttgat gggaaaaaag
     6841 cacctgtgcc aagtcttcgg gcagggcttc ggcaatgggg cgggcaagcg caaaatcttc
     6901 ggcggcaagt tcccgcactt cgccgtcagc gtttgttaag gattgacgtt gcatattttt
     6961 taacctctct tttattcgct ttgcgaaaac tgatgacacg gatgccgtct tttatcggcg
     7021 taaaacagac aatgtgcagg cgttgcgtat cgcctagata agcagcggca acataacgcg
     7081 gttcggggta atcaaagcgg acatcgggca caataacggc cgttgtccag cgtatttgcc
     7141 cgactgattc aaagggcaaa ttccgctctt cgatattgcg ttgatttttt tcggagtcaa
     7201 attcaatctt cattgcagct tgcagcgtat tttgtcgtta cattataagc ggcaaaaaac
     7261 caaaatgtaa atacaaaaaa ggaaaccccg aaatgaccat ctatttcaaa aacggctttt
     7321 acgacgacac cttgggcagc atccccgaag gcgcggttgc cgtccgcgcc gaagaatacg
     7381 ccgccctttt ggcaggacag gcgcagggcg ggcagattgc cgcagattcc gacggccgcc
     7441 ccgttttaac cccgccgcgc ccgtccgaat accacgaatg ggacggcaag aaatgggaaa
     7501 tcggcgaagc cgctgccgcc gcccgtttcg ccgaacaaaa aaccgccacg gcattccgcc
     7561 tcgcggcaaa ggcggacgaa ctcaaaaaca gcctcttggc gggctatccc caagtggaaa
     7621 tcgacagctt ttacaggcag gaaaaagaag ccctcgcgcg gcaggcggac aacaacgccc
     7681 cgaccccgat gctggcgcaa atcgccgccg caaggggcgt ggaattggac gttttgattg
     7741 aaaaagttgt cgaaaaatcc gcccgcctgg ccgttgccgc cggcgcgatt atcggaaagc
     7801 ggcagcagct cgaagacaaa ttgaacacca tcgaaaccgc gccaggattg gacgcgctgg
     7861 aaaaggaaat cgaagaatgg acgctaaaca tcggctgaaa aaatacgttt accacctgtt
     7921 ggtagccatc gaccaactgt tcaacgccct aaccggcggc gcggcggacg aaaccctctc
     7981 aagccgcacc tatcgcggcg cgcggctcgc ccaaaagccc aaaacccgct ggaaggtttt
     8041 atacaccctg atcaacggcg tatttttcga ccgccaacac tgccggcagg cgtatatcag
     8101 cgaactgaaa ggcaggcagc acgacgcgcg gttcaaccaa agccgcgccg ccggggaaaa
     8161 ggggacgcga tgaactactt cggcatggta gagtttctgc gcctgatggc aatggtgcgg
     8221 ccgccatttg tcttcttctc cggcacccgc agcgaactgc ctgcttatct tgacctcgtg
     8281 gcagaactgc gcctgaccgg atgggagcgt tttgcaggca gccaaacctt gactgtgagc
     8341 agcacatcaa cagcaattca agctacgacg accacctgat ttataagttc tgaccgcaag
     8401 tagcgtacta cttcttaaag gcataagata atccccgttt aacagaccat taaacgggga
     8461 taaatttgtg caaaagctaa tacaatttcc tacgcttcgg cggtgcaaaa gctgccgcca
     8521 attcgtgcaa aagctgccgc cgccttacat tccagtgcaa tgccgtctga aacttcgcta
     8581 atctcgggtt gccgcgcgct gtgttgttct tcggtactca gcaaaaagcc gtacagacgc
     8641 tccaatcggg cgcggtaggc ggtgaatttg ttgtagaaca tccggaagaa agaaagcgcg
     8701 ttttgcagtc gcgcgaaggc ttggacggtc tgctggatgt cgccgatttt gatttgtccg
     8761 gcaaacaggc gcggcgcttg caaaataatg gggaagagct tgatgccgtt ggtgaacata
     8821 tcattaaagc cgctcaggca gacgctttgt cgcgcaatac gccagcggtt gcggataatg
     8881 gctttaaaac ggtcagaaag ctggtcgtgt tcgtgttttt cgccgctgta aaacgccacg
     8941 ctttcggcgt ggtcgcgcac gcggataagg gaataacggt agtcgccgtt gagtttttcg
     9001 ttttcatagt tgtgacgaat caatgggttg cctatccaca tggcgataaa ggtcgccaaa
     9061 atcacaaata tatagacaaa ccaaacgatg ccgtgcggaa tgtcgaagcc gaacacggtc
     9121 aggatgccag ccaagccccg caaaacaacg gcaaattcca gagaagtaac gaccgaattg
     9181 accatgccgc gcacaaattc gatggtcgaa gcgataaatt cctgcgcgtc ctgttggata
     9241 cgctggtcga tgttgtccgg cgcgtggcgg cgcatttgca ggcggtagta gtttttgtcg
     9301 gcaagccagc gtgctgtcag cacttcgttg agccgctccg accatttaat cgccaagcct
     9361 tgatcgagga agtcgttgac gacgttgtta aacgcccgta tcagcaccac gcccgcgttc
     9421 attgcggcaa acatccaaaa tgccgaagca tttcaaatcc tgcatcgagt cgtaaagtcc
     9481 tttggacata aaggtactca acacattcag ccgtacttcg gtcaacagca gcgtaatcat
     9541 cgccgtaatc agcagcaaga ctttgaccgc gcttttcgga gtcaggcaaa ggcgcagg
//
LOCUS       DAXIEB010000077         9480 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000077 DAXIEB010000000
VERSION     DAXIEB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.077"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..1939
                     /locus_tag="ACFOA3_001498"
     CDS             <1..1939
                     /locus_tag="ACFOA3_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6898486.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQYNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGDPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
     gene            complement(2772..3455)
                     /locus_tag="ACFOA3_001499"
     CDS             complement(2772..3455)
                     /locus_tag="ACFOA3_001499"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6898487.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            3751..6054
                     /gene="parC"
                     /locus_tag="ACFOA3_001500"
     CDS             3751..6054
                     /gene="parC"
                     /locus_tag="ACFOA3_001500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6898488.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPIAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            6074..7650
                     /locus_tag="ACFOA3_001501"
                     /pseudo
     CDS             6074..7650
                     /locus_tag="ACFOA3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            7643..8110
                     /locus_tag="ACFOA3_001502"
     CDS             7643..8110
                     /locus_tag="ACFOA3_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFC6898489.1"
                     /translation="MTELQHPVLVVDNETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            8214..8966
                     /locus_tag="ACFOA3_001503"
     CDS             8214..8966
                     /locus_tag="ACFOA3_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6898490.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         2358 a         2922 c         2282 g         1918 t
ORIGIN      
        1 catcagtccc gaaatcttcg gcacgctctt ccaatcagtc atggacgcac aagagcggcg
       61 cgaagcgggc gcacactaca ccgaaaccgc caacatcgac aaagtcatca acggcctttt
      121 tttagaaaac ctgcgcgccg aatttgaagc cgtcaaagcc ctcaaacgcg acaaagacaa
      181 aaaactcgcc gccttctacc aaaaaatcca aaacctgcaa ttcctcgacc cggcctgcgg
      241 ctgcggcaac ttccttatcg tcgcctacga ccgtatccgc gccctcgaag acgacatcat
      301 cgccgaagcg ctcaaagaca aaacaggcgg cttattcgac agcccgtccg tccaatgccg
      361 tctgaaacaa tttcacggca tcgaaataga cgaatttgcc gtcctcatcg cccgcaccgc
      421 catgtggctc aaaaaccacc aatacaacat ccgcacgcaa atccgcttcg acggcgaagt
      481 cgcctgccat acgctgccgc tcgaagacgc cgccgaaatc atccacgcca acagcctccg
      541 cacaccttgg caggcggcgg actacatctt cggcgatccg ccctttatcg gctcgaccta
      601 ccaaaccaaa gagcagaaaa acgacctcga aagcatctgc ggccatatca aaggctacgg
      661 cctgttggat tacgtctgca actggtatgt caaagccgcc ggtatcatgg cgcagcatcc
      721 ccaagttcag acggcatttg tttccaccaa ttccatctgc caaggccagc aggtcgaaat
      781 cctttggggc agccttttga agcaagacat cgaaatccac tttgcccacc gcacctttca
      841 atggacgagc caagccgcag gcaaagccgc cgtccactgc atcatcgtcg gcttccgcca
      901 aaagccgcaa atgccgtctg aaaaaaccct ctacgactat cccgacatca aaggcgaacc
      961 cgtaaaacac gccgcggcca acatcaatcc gtatctgatt gacgcgcccg atgtgattat
     1021 tgccaagcgc agccgtcccg tacattgcga acctgacatg gtcaacggaa gcaaaccgac
     1081 cgaaggcggc aaccttatcc tttcaaccga tgaaaaagaa gccctgattg ccgccgaacc
     1141 cttggcggaa caatacatcc gtccctttgt cggcgcagag gaatttatca acggcaaaac
     1201 ccgttggtgc ctgtggtttc acggcgtatc cgacgttaaa cgcaaccacg acctgaaaca
     1261 gatgccccaa gttcaagccc gtattcaggc agtcaaaacc atgcgcgaag ccagcagcga
     1321 caaacaaact caaaaagatg cagcaacccc gtggcttttt caaaaaatcc gccagccttc
     1381 agacggcaat tttctgatta tcccacgcgt ctcatccgaa agccgccgtt tcgtccccat
     1441 cggttatcta tcatatgaaa cagttgtcag cgatttggta tttacccttc caaacgccag
     1501 cctctaccac ttcggcatcc tcagctccac catgcacaac gcctttatgc gtaccgttgc
     1561 cggccgtctg gaaagtcgtt accgctattc caataccgtt gtgtacaaca acttcccctt
     1621 ccccgaaagc tgccggatgc cgtctgaaaa cgaccgccaa gacccgctcc gcgccgccgt
     1681 cgaaaccgcc gcccaagccg tcctcgacgc gcgcggacaa taccgccgag aagcgcggga
     1741 agccggtttg cccgagccga ccctcgccga actctatgcg cccgacgcag gctataccgc
     1801 cctagacaaa gcccacgccg ccctcgacaa ggcagtcgat aaagcctacg gctacaaaac
     1861 aggcaaaaac accgacgacg aagccgaacg caccgccttc ctgttcgagc tgtaccgcaa
     1921 ggcggcggca attgcgtagt agttgcgtag tagttgcgta gtagttgcgt aggatagttg
     1981 cgtagggtgg gcttcagccc accaactccc accaactccc accaactccc accaactccc
     2041 accatcttca ccctaaaacc ccgtcatctc cgcaaaagca attgcgtagg gtgggcttca
     2101 gcccaccgtt tttcttcatt ttcggttgtt gattggcagg ctgaagccca ccctgcggtt
     2161 acaaaaatcc cgtcattccc gcgcaggcgg gaatccagac ctttcggggc ggaaacttat
     2221 cggataaaac gatttcccct gcatcctgga ttcccacttt catgggaatg acgggatata
     2281 ggtttcccta cggacgtgtt cagattcccg ccttcgcggg aatgacggcg gagaagtttc
     2341 tgtctttccg ataacttacc gtaacttaaa atcccgtcat taccgcaaaa gcagttgcta
     2401 cggttacaaa aatcccgtca tccccacgaa agcgaaaatc ctgcctgtcg gatttcggtt
     2461 ttttcgggcg tttcgggaac ttataaatcg tcattcccgc gcaggcggga atccagaacg
     2521 cgggacggcg gcaatattca aaggttgtct gaaaattcag aggttctaga ttcccgcctg
     2581 cgcgggaatg acggcggagc ggtttctgct tttccgataa atgaccgcaa cctaaaaccc
     2641 atccttccca cgaaaacaga aaaacaaaaa cctaaaatcc cgtcgttctc gcgaaagcag
     2701 ttgctacgtt taccgtcaaa aatgccgtct gaacgctcaa gccttcagac ggcatttttt
     2761 atttcacacc cttacaggta gaatttttcg atgactttca aattgtcgtc caatttgtac
     2821 accaacggct gaccggtcgg gatttccaag cccataatgt cttcgtcgga aatgccctcg
     2881 atgtgttttg ccagcgcgcg cagggagttg ccgtgcgccg ccaccaagac gcgtttgccg
     2941 ctcaaaatcg cgggggcgat ttggtcttcc caaaacggca atacgcgctc cagcgttact
     3001 tgcaggtttt cgccgtcggg tacgacatcg gcaggcagat gggcatagcg gcggtctttg
     3061 tgtgcggaaa actcatcgtc tttgtccaaa agcggcggca gggtgtcgta gctgcgccgc
     3121 cagatgcgga cttgctcgtc gccgtattgt tcggcggttt gttttttgtc caggccttgc
     3181 agtcggccgt agtggcgttc gttcagccgc catgttttga tttgcggtac gaacagttgg
     3241 tcggattctt ccaaaacgat gttgcaggtc ttaatcgcgc gggtcaggac ggatgtgaag
     3301 gcgatgtcga actcatagcc gttttctttc agttttttac cggcggcagc agcctcggca
     3361 agcccctgct cgctcagctt cacgtcgcgc cagcctgtaa acaggttttt cgcgttccat
     3421 tcgctttgtc cgtggcggat aaataccaat tccatatcgt ctccaatgtg tgaaagtggg
     3481 aaagccttat ttataacata ttttcacatt cccgtatttg attcagattc agacacgcgc
     3541 ccactatggt ttgccgtttt gatttacaat aatgcccttt gctttacatt ccgcatacac
     3601 aatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgttgcc ctgatttgga
     3661 aggggttgcc ccctcccaaa taaagtctga ttctaccgcc ccgaaggaca gatgtccaag
     3721 tggcgggggt tcaaccgaaa aggaaatacg ataaatatgc aaccgcacgc tccacatacc
     3781 gattccaata cgctgatgct cggccgatac gccgaacgcg cctatctcga atacgccatg
     3841 agcgtggtca aaggccgcgc gctgcctgaa gtttcagacg gccaaaagcc cgttcagcgg
     3901 cgcattttgt ttgccatgcg cgatatgggt ttgacggcgg gggcgaagcc ggtgaaatcg
     3961 gcgcgcgtgg tcggcgagat tttgggtaaa taccacccgc acggcgacag ttccgcctat
     4021 gaggcgatgg tgcggatggc gcaggatttt accttgcgct atcccttaat cgacggcatc
     4081 ggcaacttcg gttcgcgcga cggcgacggt gcggcggcga tgcgttacac cgaagctcgg
     4141 ctgacgccga ttgcggaatt gctgttgtcc gaaatcaatc agggaacggt ggatttcgtg
     4201 ccgaactacg acggcgcgtt tgacgagccg ctgcacctgc ccgcccgcct gcctatggtg
     4261 ttgctcaacg gcgcgtcagg catcgcggtg ggcatggcga ccgagattcc gtcgcacaat
     4321 ttgaacgaag tgacgcaggc agcgattgcg ctgttgaaga agccgacgct ggaaaccgcc
     4381 gacctgatgc aatatattcc tgcgcccgat tttgccggcg gcggtcaaat catcacgccg
     4441 gcggacgaat tgcgccggat ttacgaaacc ggcaaaggca gcgtgcgcgt gcgtgcgcgt
     4501 tatgaaatcg agaagctggc gcgcggacag tggcgcgtca tcgtaaccga gctgccgccg
     4561 aacgccaatt ccgccaaaat ccttgccgaa atcgaagaac aaaccaaccc gaaaccgaaa
     4621 gcgggtaaga aacagctcaa ccaagaccag ctcaacacca aaaagctgat gctggattta
     4681 atcgaccgcg tgcgcgacga gtccgacggc gaacatcccg tgcgtctggt gttcgaaccg
     4741 aaatccagcc gcatcgatac cgataccttc atcaacacgc tgatggcgca aacttcgctg
     4801 gaaggcaatg tgtccatgaa cttggtgatg atggggctgg acaaccgccc cgcgcagaaa
     4861 aacctgaaaa cgattttgca ggaatggctg gatttccgca ccgtaaccgt aacacgccgt
     4921 ctgaaattcc gtttgaacca agtggaaaaa cggctgcaca tcctcgaagg ccgtctgaaa
     4981 gtctttctgc acatcgacga agtgattaaa gtcatccgcg aatcagatga cccgaaaacc
     5041 gatttgatgg cggcgttcgg gctgaccgaa atccaagccg aagacatttt ggaaatccgc
     5101 ctgcgtcagt tggcgcgttt ggaaggtttc aaactcgaaa aagaattgaa cgaattgcgc
     5161 gaagaacaag gccgtctgaa catccttttg ggcgacgaaa acgaaaaacg caagctgatt
     5221 atcaaagaaa tgcaggcgga catgaaacag ttcggcgacg cgcgccgcac gctggtggaa
     5281 gaagccggac gcgccgtgct gacacaaacc accgccgacg aacccatcac actgattctg
     5341 tctgaaaaag gttggatacg cagccgcgcc ggacacaatc tcgatttgag ccaaaccgcg
     5401 ttcaaagaag gcgaccgcct caaacaaacc ctcgaaggca gaacggtttt acccgtcgtc
     5461 attttggatt catcgggcag aacctacacc cttgatgccg ccgaaatccc cggcgggcgc
     5521 ggcgacggcg tgccggtttc atccttgatc gagctgcaaa acggtgcgaa acccattgcg
     5581 atgttgacag gattgccgga acaacattat ttattatcaa gcagcggcgg ctacggcttc
     5641 atcgccaaac tgggcgatat ggtcgggcgc gtgaaagcgg gcaaagtggt gatgaccgca
     5701 gacagcggcg aaaccgtttt gccgccggtt gccgtctatg cctcctcgtt catcaacccc
     5761 gactgcaaaa tcattgccgc caccaatcaa aaccgcgccc tcgccttccc catcggcgaa
     5821 ttgaaaatta tggcgaaagg caaagggctg caaatcatcg gattaaacgc cggcgaatcg
     5881 atgacgcata ccgccgtttc ttccgagccg gaaatcctga ttgaaagcga aggcaggcgc
     5941 ggcgcagcgc acaaagaccg catccccatc tccctgcttg aggcaaaacg cggcaaaaaa
     6001 ggcaaactat tacccatatc gggcagcctg aaacagcttt cttccccgaa ataaacccga
     6061 ctctgcacat actatggtaa tttccaatac ccgcgaactt gagaaactca aagaccggat
     6121 tcccaatctg atcaacatcg cccgcatcgc catcgtcttc cccctgctga ttatgcatat
     6181 cttcgggttg gaggtcagcg gacatgaaaa cctgcacgct tcgtggacgg cgtgggcatt
     6241 ttatgtttgg ctcgccattt cctgctggct gattttcttt tccatcatcc atccgcattg
     6301 gcaatggcag tcgctgaaaa tgccgcgttt cagcgcggtg gcggacatca cgatgatcgg
     6361 cgtgctgacc tacctgttcg gcggcatcga ttccggcttc ggcatcctga ttctgccctt
     6421 cgtcgtctgc tcctgcctgc tcagctacgg gcgttacccc ctgctctatg ccagctacgc
     6481 cgccatcctg ctgatattga atgcccttgc agacagcaat atcaatatgt atccgctgat
     6541 tctggatgca aaaaccgtca ccaacacctt cgtcctcgtg gctggttcct atttcgtcgc
     6601 catgattgcc tcactgtcgg tcagatacat cgaccgtgcc ggaaaactcg cctacgacag
     6661 ccatatcgcc taccggcgca tcaaaggctt gagccaaacc gtactcaacc gcgttcagga
     6721 agcggtcgtc gtcatcaacg tcgagcatca gaccatactg ttcaataaaa aggcaaaaga
     6781 cctgctaccc atgcttgaaa tcggacagca tactgccctg ttcgacccta ttaccgtttt
     6841 atgggacaag gcctcttcgc gtactttcga acgcaatatc gacacgcccg gcctgaacgc
     6901 cggcatccgc gccgttccgg tcaacaaagg gccggacaag ctgctcatcc tctacatccg
     6961 cccgcaaagc gaaattcagg cagaagccct gtccgtcaaa cttgccgcgc tcggacaact
     7021 gaccgccaac ctcgcccacg aaatccgcaa cccgatgtcc gccatccgcc acgccaacga
     7081 cctgctgcgc gaaaatatgg aagcgggggc ggcagatccg ttcaacgcca aattgtgcaa
     7141 aatcatcgac ggcaacgtct gccgtatcga caaaatgctc gaagacattt cctcgctcaa
     7201 caagcgcaac aaaaccgaac gcgaaaccat cagcctgata ccgttttggg aagaattcaa
     7261 acaagagttc ctgctcggcc atcccgatgc cgccggctgc atccgtccga acattcaggg
     7321 cggcagcctg accgcctatt tcgatcccgc ccacctgcga caaattatgt ggaacctcgc
     7381 caacaacgcg tggcgacaca gccgcaaaca gcccggctca atttccgtca ccatccgccc
     7441 cgcgcaaaaa aacaccgtct gcatcctctt tgccgaccgc ccgaagtgca ggaacacctg
     7501 ttcgaaccct tttacaccac ggcggaaaac ggcaccggcc tcgggctgta tgtcgcccgc
     7561 gaactggcgc acgccaattt cggcgatttg acctacctgc ccgaagccaa atgtttcgaa
     7621 ctcgcattac cggaaaaaac caatgactga actgcaacac cccgtcctcg tcgtcgataa
     7681 cgaaaccgac attctcgacc tgatggaaat gaccctgatg aaaatgggct tgcgcgtcca
     7741 taccgcgtca ggcgttgccg aagccaaaaa caagctcgac agccaacgct attcgctcgt
     7801 cctgaccgat atgcgtatgc cggacggctc ggggctggaa gtcgtccaac acatcaacag
     7861 ccgcctgctc gatacgccgg ttgccgtcat caccgccttc ggcaacgccg atcaggcaca
     7921 ggaagcgttg cgttgcggcg cgttcgaccc cgataccatg cagatacagg actatctcga
     7981 ccaaatcgaa cgcgacatta tcgaacaaac cctcaaacaa accgaaggca accgcacgca
     8041 ggccgccaaa cgcttgggca tcagcttccg ttccatgcgc taccgtatgg aacgcctcaa
     8101 catcggctga cgacaaaacg gcatccgcac catctccgcc cacccgaaaa aatgccgtct
     8161 gaaacggcac gggaaagcgg gttcgcccca cgcccgaacg gacacaaaac accatgaccg
     8221 acatccttat tgacaacacc gccaccgaaa ccgtccgcac cctgatacgg gcattccccc
     8281 ttgtgcccgt ttcccaaccg cccgaacaag gcagctacct ccttaccgaa cacgataccg
     8341 tcagcctcag gcttgtcggg gaaaaaagca gcgtcatcgt cgactttgcc tcgggagccg
     8401 cacaataccg gcgcacaaaa ggcggaggag aactcatcgc caaagccgtc aaccataccg
     8461 ccaaacccac cgtatgggat gcaaccgcag gattggggcg cgacagcttc gtcctcgcct
     8521 cgctcggact gaccgttacc gccttcgagc agcatcccgc cgtcgcctgc ctgctttcag
     8581 acggcatccg ccgcgccctc ctcaatcccg aaacgcaaga caccgccgcg cgcatcaacc
     8641 tccatttcgg caacgccgcc gaacagatgc ccgcccttgt tcaaacacaa ggcaaacccg
     8701 acatcgtcta tctcgacccc atgtatcccg aacgccgcaa aagtgccgcc gttaaaaaag
     8761 aaatgaccta cttccatcgg ctcgtcggcg aagcgcaaga tgaagcggca ctcctgcata
     8821 ccgcacgcca aacagcaaaa aaacgcgtcg tcgtcaaacg cccccgcctc ggcgaacacc
     8881 ttgccggaca agcccctgcc taccaataca ccggcaaaag cacccgcttc gacgtttacc
     8941 tgccctacgg gacggacaag ggataacgcc cataaaacaa gacaccgaaa aatttgccgt
     9001 tcttatgcaa acgagaaacc ggtttttgcg tttcgactgt tttggataaa tcatcacacc
     9061 ttaaagtttg tcattcccac ggaagtggga atccgattca ttcagtttta tagtggttta
     9121 aatttaaacc actatagctg ttttcgagtt tcaggcaact tccaaaccgt cattcccacg
     9181 gaagtgggaa tctagaaatg aaaggcaaca ggaatttatc gtaaatgacc gaaaccgaac
     9241 ggactagatt cccgcctgcg cgggaatgac ggggcgggcg gatgccgtct gaaattccgt
     9301 cattcccgta aaaacgggaa tctagaactt ctgatttttc agacgacttt tgaacattgc
     9361 cgctacccaa tgatctggat tcccgcctgc gcgggaatga cgaggtttca ggttgctgtt
     9421 tcgggttgct gttttttatg gaaatgacga ggttttagat tgcgagaatt tatccgctcc
//
LOCUS       DAXIEB010000078         9406 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000078 DAXIEB010000000
VERSION     DAXIEB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.078"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            130..696
                     /locus_tag="ACFOA3_001504"
     CDS             130..696
                     /locus_tag="ACFOA3_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6898491.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRVLGGLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
     gene            complement(1372..3408)
                     /locus_tag="ACFOA3_001505"
     CDS             complement(1372..3408)
                     /locus_tag="ACFOA3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6898492.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVTDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFALYFDDAAPLAGIPKDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLACRAKPYAEKDLAEVKAFARERLNLADPQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVSKVLAGLFAQIKKLYGIGFTEKTVPIWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFTPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(3496..4368)
                     /locus_tag="ACFOA3_001506"
     CDS             complement(3496..4368)
                     /locus_tag="ACFOA3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6898493.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            4638..7028
                     /gene="gyrB"
                     /locus_tag="ACFOA3_001507"
     CDS             4638..7028
                     /gene="gyrB"
                     /locus_tag="ACFOA3_001507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6898494.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYNADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            7291..8667
                     /locus_tag="ACFOA3_001508"
                     /pseudo
     CDS             7291..8667
                     /locus_tag="ACFOA3_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
     gene            complement(8738..8836)
                     /locus_tag="ACFOA3_001509"
     CDS             complement(8738..8836)
                     /locus_tag="ACFOA3_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC6898495.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(8971..>9406)
                     /locus_tag="ACFOA3_001510"
     CDS             complement(8971..>9406)
                     /locus_tag="ACFOA3_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898496.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFECFGKYNLVQTEYRRFKVDKT
                     ENRNHKANSIFEYLHILEKTFYSGLTKTSTALPRLSSKRTIL"
BASE COUNT         2176 a         2483 c         2641 g         2106 t
ORIGIN      
        1 gcgggaatga cgggatttct gtttttgatt ttttgttttt gcaggaatga cgggatgtgg
       61 gttttcgtgc ggcgtttttc gcgctttgct gttttgctat aatctgccct ttttgaggac
      121 gggtgcagta tgagtcttta cgctttgctc ttgattgcgc tggggatgtc gatggatgcg
      181 tttgccgtcg cattggcaaa gggtgcggcg gtcaggatgc ccccgcgcaa aattgcagca
      241 acggctttgg tgttcggcac ggttgaagcg ctcacgccgc tggcaggctg ggtaggcggt
      301 ttttatgcca agccgtttat cagcgaatgg gaccattggg cggctttcgt cctgctgggc
      361 gggctgggtc tgaaaatgat gcgcgaaggg ctgtccggca aggcggaaga tgtgcgcgaa
      421 agcaaacggg aaagcctatg gatgacggtt ttgactgctt ttggaaccag tattgattcc
      481 atgatagtcg gggtgggctt ggcgtttatg gaggtaaaca tcgcctttgc cgccgcaatc
      541 atcggtatgg cgacgacggt gatggtggcg gtcgggctga cggcggggcg agtattgggc
      601 ggattgttcg gcaggcgtgc ggaatttgcc ggaggtttgg tgttgattgc catcggcaca
      661 tggacgctct tgtcgcattt gggtttgatt caatgatgtc ggaaaatata gtggattaac
      721 aaaaaccagt acgtcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctga
      781 agcaccaagt gaatcggttc cgtactattt gtactgtctg cggcttcgtc accttgccct
      841 gatttttgtt aatccactat aaaatgccgt ctgaagcgtt gtttgaccgt ttcagacggc
      901 atttttatca aattcaaaaa tattccgtca ttcccgcgaa agcgggaatc tagaacgtaa
      961 aatctaaaga aaccgtttta tccgataagt ttccgcaccg acagaccttg attcccgcct
     1021 gcgcgggaat gacgggattt ctgtttttga tttttttgtt tttggggaat gacgggattt
     1081 gagattgcgg gcatttatcg ggtaaaacgg aaattaaacg ttgcgaaaat ttatccgaaa
     1141 tcacagcaac ttttccgcgt cattcccacg aaagtgggaa tctagaacgt aaaatctaaa
     1201 gaaaccgttt tatccgataa gtttccgcac cgacagacct ggattcccgc ctgcgcggga
     1261 atgacgggat tttggcttac tgtttttgtg aaaatggcga aaccgcgcaa atgctgatgc
     1321 cgtctgaaac actgtttgat tgtttcagac ggcatttttt gttcacatcg atcaagccgc
     1381 gttgtcgaag ccgctgtggc gcaagagtgc gtctatgctc ggttcgcgtc cgcggaaggc
     1441 tttgaaggac tctgccgcgc tgcgggagcc gccgacggcg aggatttcct gccaaaagcg
     1501 tttgcctgtg gcggcgacat cgtcgctttc ttcaaaggcg gcgtatgcgt ccgcgctcaa
     1561 tacttccgcc cacgcgtagc tgtaatagcc tgcggaatag ccgcctgcga agatgtggcc
     1621 gaagctcaag gcgaagcggt tgtattcggg cggctggatg acggcgactt ttttgcgcac
     1681 gctgtctaaa acctgttgcc agtttttcag acggccttcg tcgtcttcgc tgtaaatcat
     1741 catatcaaag agggcgaact ccatttggcg gacgaggaac atgccgcgtt ggaagttttt
     1801 ggcggcgagc attttgtcga agagttcttt cggcagggga acgccggttt cttcgtgggc
     1861 ggacatttgc gccaagacat tgtattccca aacgaaattt tccataaact ggctgggcag
     1921 ttcgactgcg tcccattcta cgccgttgat gccggatacg cccagttcgt cgacttgggt
     1981 aagcaggtgg tgcaggccgt gtccggtttc gtggaagagt gtgaggattt cgtcgtggct
     2041 caaacgcgct tctttgccgc cgacgggcgg ggtgaagttg cagacgaggt aggcggtggg
     2101 cagttgcagc gtgccgtcgg cgaagcggcg gcggcctttg tagtcgttca tccatgcgcc
     2161 gccgcgtttg ccttcgcgtg cgtacaaatc catataaacg ccgcctatgg tttcgccgtt
     2221 ttgttgcaat tcaaaatagc gcacgtcttt gtgccagatg gggacggttt tttcggtaaa
     2281 tccgatgccg taaagttttt tgatttgggc gaacaggccc gccagaactt tgctgacggg
     2341 gaagtatttt ttgacttcgg tttcgctgaa tgcgtatttg gcttcgcgca gtttttcgcc
     2401 ggcgtagccc aagtcccacg gctgcgggtc ggcgaggttc aggcgttcgc gggcgaaggc
     2461 tttgacttcg gcgaggtctt tttcggcgta gggtttggcg cggcaggcga ggtcgtgcag
     2521 gaagtttaaa acttgttcgg gggtgtccgc cattttggtt gccagcgaca attcggcgta
     2581 gtttttgaag ccgagcagtt tggcggtttg cagggcgttt gcgagcgtgc ggtcgatgtt
     2641 ggcggtgttg tcgaatttgc cgccgtctga aagttcgctg gcgcgggtaa cgtaggcgcg
     2701 gtagatttgt tcgcgcagtt cacggttgtc ggcgtattgg atgacggcga ggtagtgcgg
     2761 aatctgcaaa ccgattttgt agcctgtttt gccttcgctt tgcgcggcgg cggcaaacat
     2821 ggcgagcgcg tctttgggaa tgccggcaag cggtgcggca tcgtcgaagt agagggcgaa
     2881 ggcgtcggtt gcgtctaaga cgttttggga gaatttggcg gaaagttgcg cgccttcggt
     2941 ttgcagtttt gccagttctg cctgctgttc gggcggcagt tccgcgccgc taaggacgaa
     3001 atcgcgcagg tcgtggttga gcttggtttt ttgtgcgggg gagagggtgt cgaattcggg
     3061 ggaatttttg atggttttga agcggttgta cagctcgatg tcttgtccga tttcggtgaa
     3121 gaagacggtg atttcgggca tcagttcgtt atagacggcg cgcagttcgg gcgtatcggt
     3181 gacggagttg aggtgcgaca ccacgcccca aatcctgccg acgcgttcgg tgatgccggt
     3241 caggggttcg acagtgtttg cccagccggt gtgcgtttgg gctttgatgg cggcgatttg
     3301 ttcgcgcgct tcggcaatgg cggtttgcag ggcgggtttg atgtcttcgg ttttgatttg
     3361 atcaaaacgg ggttcttcgc ccaaatggag cagtgcgttg tcagtcataa gatgggtttc
     3421 ctttgcgtgt tgtgcttcag acagcatatc ggcaatatgc cgtctgaagc cggaaatggg
     3481 gtcggtcagg gaaagttaaa gtgtttcgga gccggcgggg taatttaaca ggcggtattg
     3541 tacgcgcaaa aattcgtcca taaccgcctg ttggatttcc agccgctttg aaacggggga
     3601 ggcgaagcgg acgatgatgc ggtatgcctt gtcatcgtac ggcacgcggg taacgcgcgg
     3661 tctggcggcg ggcgtgataa acagtttttc cgcctgcacg ttttccaaat gccgttggat
     3721 ggcggggatg tagggcgcgc acaagggctc gagtacggct ttcagacggc atacggcttc
     3781 atccgaatcc aaatggatgg gaacggggat ttcgaccgta tggatgacat agtcgcccaa
     3841 aatattgtcg cggcgcacgg ggtggctcaa caacaggctg ttggggaaag aaacggtggt
     3901 tcccgcaagc tgtccgacca aggggttcgg accgacctgc atcatcagcg tgttcaacag
     3961 gttgatgtcg accacgcgcc cgcgcaggcc gttgatttcg atatagtcgc cgaccgagta
     4021 ttgctgggtg gcagacctta aaatactgcc cgacagacac ataatcagtt ccttcgtcgc
     4081 cacgacgacc gccgccgcca ccgcaaacat cgacaaagcc agcgtttgga tttgcgccga
     4141 ccagataaat gccagcgaaa acagcaccaa aagcagcgtt atattgcggc tggcaaccaa
     4201 aaaccgccgc ttgctttcga tgccgaaatc cggatgccgt ttgaagtgga tattcaacag
     4261 aagggcgcgc gccagcagca aagccgcaac cgccgccacg gattcgaccg cctccgcacg
     4321 tatcgggacg gcaccgagcc aagtgtccaa catattccat atttccattt ccgcgcccct
     4381 gtccgaaaag tatagggtgt agattttagt ggcaaaaaaa cgcttttgcc actttacggg
     4441 aaaatccccg acctgatgcc ggaggcaaac gtaccgtttc atcttttccc ggcggcacac
     4501 tgatgcggat aacgctgaat taggcggcaa aatcggctat aatacacaat tatatatgcc
     4561 gtcccgcccg ctttttccac aaggtgcgga cttgttttgg cggcatcgca aaatcttttc
     4621 aaaatccggc aaaaaatatg actgaacaaa aacacgaaga atacggcgcc gacagcattc
     4681 aggtgctcga aggcttggaa gcggtacgca aacgccccgg catgtacatc ggcgacacgc
     4741 aggacggcag cggtctgcac catatggtgt ttgaagtatt ggacaacgcc attgacgaag
     4801 cactcgccgg acattgcgac aaaatcacgg taacgataca cgccgaccat tccgtcagcg
     4861 tcgccgacaa cgggcgcggt atgcccaccg gcatccaccc gaaagaagga cgctccgccg
     4921 ccgaagtcat catgaccgta ttgcacgcgg gcggtaaatt cgacaacaac agctacaaaa
     4981 tctccggcgg cctgcacggc gtgggcgtgt ccgtcgtcaa cgcgctgtcc gactgggtaa
     5041 cgctgaccat ctaccgcgac ggcaaagaac acttcgtccg cttcgtgcgc ggcgaaaccg
     5101 aagagccgct gaaggttgtc ggcgattccg ataaaaaagg cacgaccgtg cgcttcctcg
     5161 ccagtacgga aaccttcggc aacgtcgaat acagcttcga catccttgcc aaacgcatcc
     5221 gcgaactttc gttcctgaac aacggcgtgg acatcgaatt gaccgacgag cgcgacggca
     5281 aacacgaaag cttcgccctt tccggcggcg tggctggatt cgtgcaatac atgaaccgca
     5341 aaaaaacgcc cttgcacgaa aaaatcttct acgcgttcgg cgagaaagac ggcatgagcg
     5401 tcgaatgcgc gatgcaatgg aacgacagct atcaggaaag cgtgcagtgc ttcaccaaca
     5461 acatccctca gcgcgacggc ggtacgcacc tgaccgcgtt gcgccaagtg atgacgcgca
     5521 ccatcaacag ctacatcgaa accaacgaag tcgccaaaaa agccaaagtg gaaaccgccg
     5581 gcgacgatat gcgcgaaggt ttgacctgcg tgttgtccgt caaactgccc gaccccaaat
     5641 tttcgtccca aaccaaagac aaactggttt ccggcgaaat cggtcccgtc gtcaacgaag
     5701 tcatcagcca agccctgacc gacttcctcg aagaaaaccc tgccgaagcc aaaatcatca
     5761 ccggcaaaat cgtcgatgcc gcccgcgcgc gcgaagccgc ccgcaaagcc cgcgaaatca
     5821 cccgccgcaa aggcgtgatg gacggcttgg ggctacccgg caaactcgcc gactgccaag
     5881 aaaaagaccc cgcattgtca gaactttatc tggtcgaggg cgattctgca ggcggttccg
     5941 ccatgcaggg gcgcgaccgc aaattccaag cgattttgcc gcttaagggc aagattttga
     6001 acgtcgaaaa agcgcgcttt gaaaaaatgc tcgccagtca ggaagtagcc gctttgatta
     6061 ccgcgttggg cgcgggcatc ggcaaggaag aattcaatgc cgagaagctg cgttaccacc
     6121 gcatcattat catgaccgat gccgatgtgg acggcgcgca tatccgcacc ttgcttttga
     6181 ccttcttcta ccgccaaatg cccgaactgg tcgagcgcgg ctacatctat atcgcccagc
     6241 cgcctttgta taaagcgaaa tacggcaaac aggaacgtta cctcaaggac gagttggaaa
     6301 aagaccaatg gctgctcggt cttgccttgg aaaaagccaa aatcatttca gacggccgca
     6361 ccatcgaagg cgcagaactt gccgacaccg ccaaacaatt cctgttggca aaaaccgtca
     6421 tcgaacagga aagccgcttc gtagacgaac tcgtcctgcg cgccatgcta cacgcgtcgc
     6481 ccattgattt gacgtcgtct gaaaacgccg ataaagccgt tgccgaactt tccggtctgc
     6541 ttgacgaaaa agaagtcgcc ctcgaacgca tcgaaggtca tgaaggacac cggttcatca
     6601 aaatcacgcg caagctgcac ggcaacgtca tggtcagcta catcgaaccc aagttcctca
     6661 acagcaaagc ctaccaaacc ctcacccaaa ccgccgccgc gctcaaaggc atggtcggcg
     6721 agggtgccaa gctttacaaa ggcgaaaacg ggtacaacgc ggacagcttt gaaaccgctt
     6781 tggacatctt gatgagcgtt gcccaaaaag gtatgtccat ccaacgatac aaaggcttgg
     6841 gcgagatgaa ccccgagcag ctgtgggaaa ccacgatgga tcccgccgtg cgccgcctgt
     6901 tgaaagtgcg catcgaagat gccattgccg ccgacgaagt gttcgttacg ctgatgggcg
     6961 acgaggtcga gccgcgccgt gcctttatcg aaaacaacgc gctgattgcc caaaatatcg
     7021 acgcataagt gccgttttaa aaaaggagac gggcatcgtg ccgcgtctcc tttttggttg
     7081 gtcaaacgga agccataccg tctgaaaccg cctttcggag caaaaccatg gtcagcattt
     7141 ttgatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacaaata
     7201 gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag
     7261 gcgaggcaac gctgtactgg tttaaattta atccactata ttttcaaaat cggtatcggg
     7321 ccttccagtt cgcatacggt cggcccgatg aaggcagccg ccgcctttgc ggcaggtttg
     7381 gatgcacagg ctgttcgcat cgtcatcgac atttacggct cgctcgcact gaccggatac
     7441 ggacacggta catttgacgc gctgatgctc ggtttggaag gcagcctgcc gcacgacatc
     7501 ccgcttgccg gcattcccga acgcctcgaa cgcatccgca cgcagcacat cctccggctc
     7561 aacgggcaag aaatccgctt catccccgac cgcgacctga acatactcgg caatcaagtg
     7621 ctgcccaaac accccaacgg cctgcgtttt accgcctatg cttcagacgg cacggtattg
     7681 aatgaacagg tttattattc ggtcggcggc ggctttgtcg ttaccgaaga agattttgac
     7741 cggcaggcgg aaacggaaaa agccgttccc tatccctata ccagttgcgc cgaactgctt
     7801 gcccgatgcc gtctgaaccg gctcgacatc tccgaagtcg tgttggcaaa cgaagccgcg
     7861 cttgccggat gcggcgaagc cgaaatccgc cgccgcgccg ctgccgttgc cgaggttatg
     7921 gaaggctgca tcaaacgcgg cttgggtgcg gacggcgaac tgcccagctg attgaacgtc
     7981 cgccgccgcg ccccgcagct tgccgccaag ctcaaagtcc tgcgcgaaac cgaaatcgtc
     8041 aacacccagc tctggccgat ggtgtacgcc atggcggtca acgaagaaaa cgccgccgac
     8101 ggacgcgtcg ttaccgcacc gaccaacggc gcggcaggca tcattcccgc cgtattgcac
     8161 tatttccgca agttcaatcc gcacgccaca caggaacgcg tcgaaaactt cctgctcacc
     8221 gcaggcgcaa tcggcatcct ctacaagacc aacgcctcca tttccggtgc ggatgtcggc
     8281 tgtcagggcg aagtcggcgt agcgtgttcg atggcggcgg gcgcatacgc cgaagtcatc
     8341 ggcggcacgc ccaaacaagt ggaaaacgcc gctgaaatgg cgatggaaca ccatttgggg
     8401 ctgacttgcg accccgtcgg cgggctggtg caaatcccct gtatcgaacg caacggcatc
     8461 gccgccgaaa aagccctcaa actcggcacg ctcgcgcttt tggaagacgg cacggacaaa
     8521 aaagtctcgc tcgacgaagt catccgaacc atgctgcaaa ccggccggga tatgaaggcg
     8581 acctacaaag aaacctcgct tgccggactc gccgccacgc tccggaaaaa agccgtcccc
     8641 gtatccgtgc gcgtggtcga gtgctgagga tggacagaaa cgaaaatgcc gtctgaaaac
     8701 agttttttag acggcatttc atttatttct tgcggcttta ccgtttcagt gccagcgtca
     8761 acacgcccgc cgtaaccagc cccaagccta tccattcctg cgtgttcggg cgttcgtcta
     8821 agaaaaccac cgccatcagg aatgcaaacc ggcgttgttt gcagcatccg gcaagaattt
     8881 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
     8941 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
     9001 aggcaacgct gtactggttt ttgttaatcc actataaaag gtcttttcta aaatatgcag
     9061 atattcgaat atcgaatttg ccttatgatt acggttttct gtcttatcta ccttaaaacg
     9121 ccggtattcc gtttgaacca aattatattt gccgaaacac tcaaaaatct cccttacctg
     9181 cccgacagac atcaagcctt cgttgttgta gctgagaaaa atatattgaa agtccgcatc
     9241 cctaatcaaa gcctcaaata ccggaaccac atccgatttt gagcaagtcc gcgctaaaac
     9301 acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc ggaaacaaac atttttcaaa
     9361 gcatacaaac aaaaaaatac caaccggagg ttggtattcc ttaact
//
LOCUS       DAXIEB010000079         9312 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000079 DAXIEB010000000
VERSION     DAXIEB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.079"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            236..1189
                     /locus_tag="ACFOA3_001511"
     CDS             236..1189
                     /locus_tag="ACFOA3_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6898497.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNAESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
     gene            complement(1431..2678)
                     /gene="fabF"
                     /locus_tag="ACFOA3_001512"
     CDS             complement(1431..2678)
                     /gene="fabF"
                     /locus_tag="ACFOA3_001512"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6898498.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(2834..3070)
                     /gene="acpP"
                     /locus_tag="ACFOA3_001513"
     CDS             complement(2834..3070)
                     /gene="acpP"
                     /locus_tag="ACFOA3_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6898499.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            3302..4309
                     /locus_tag="ACFOA3_001514"
     CDS             3302..4309
                     /locus_tag="ACFOA3_001514"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6898500.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGEDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            4325..4504
                     /locus_tag="ACFOA3_001515"
     CDS             4325..4504
                     /locus_tag="ACFOA3_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898501.1"
                     /translation="MPSKRTFCRSDGIFISFFRLPPLENPLRAALFEIRQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            4642..4965
                     /locus_tag="ACFOA3_001516"
     CDS             4642..4965
                     /locus_tag="ACFOA3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898502.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            5052..5333
                     /locus_tag="ACFOA3_001517"
     CDS             5052..5333
                     /locus_tag="ACFOA3_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898503.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            5386..5859
                     /locus_tag="ACFOA3_001518"
     CDS             5386..5859
                     /locus_tag="ACFOA3_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6898504.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            5968..6204
                     /locus_tag="ACFOA3_001519"
     CDS             5968..6204
                     /locus_tag="ACFOA3_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898505.1"
                     /translation="MKRLEDMIMGFWDSAANLAKEAGEGVKRFNERSRKLQNEYQQES
                     SEKLRRVVNSEGFFGNSAQEKAIARKVLKERGEG"
     gene            6401..9091
                     /gene="glnE"
                     /locus_tag="ACFOA3_001520"
     CDS             6401..9091
                     /gene="glnE"
                     /locus_tag="ACFOA3_001520"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6898506.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEERQGRLKEHGFDAETIAAR
                     LDQIRNGHKYRHLSAHAQLRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDALRRFQHAQVFRLAVQDLAGLWTVEPLSDQLSALADTILAAAL
                     PCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTVLAGEIIEMRERMFPTHPPVDSNVKYAHGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
BASE COUNT         2189 a         2329 c         2571 g         2223 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gctgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg tgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacgggattg agcgcgaaat tttttccggg
     1081 caacgccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gccccgcctt
     1261 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat
     1321 ttgtactgtc tgcggctcgc cgccttgtcc tgatttttgt taatcactat aaaaatgccg
     1381 tctgaaacgg tttcagacgg catttcgatg tcggcggcgg ctttgcggaa tcagcctttg
     1441 aagcgtttga agaccagcgt gccgttggtg ccgccgaagc cgaaggagtt ggaaatggca
     1501 acgtcgattt ccgcgtcgcg cgcttcgttg gcgcagtagt ccaaatcgca gccggcttca
     1561 acgtcttgtt caaaaatgtt gatggtcggc gggattttgc cgtcgtgtat cgccaaaatg
     1621 ctgtacacgg cctccacgcc gcccgccgcg ccgagcaggt ggccggtcat ggatttggtc
     1681 gagctgacga cggttttgta ggcgtgttcg ccgaacgcgc gtttgagggc tttggtttcg
     1741 ttggcatcgc ccaagggggt ggacgtgccg tgcgcgttga cgtaatccac gtcttcggga
     1801 ttgatgccgg catctttcag cgcgcgggta acggcaaggg cggggccttc ttcgttcggc
     1861 gcggtgatat ggtaagcatc ggaactcatg ccgaagccga cgatttcggc gtagattttc
     1921 gcgccgcgtt ttttggcgtg ttccaattct tccaacacca atatgcccgc gccttcgccg
     1981 ataacgaagc cgtcgcggcc tttgtcccac ggacgggaag cggtggcggg gtcgtcgttg
     2041 cgggtggaga gggctttcat cgcggcaaaa ccgcccacgc ccaaagtgct gattgcgcct
     2101 tccgcgccgc cggcaaccat tatgtccgcg tcgccgtatt tgatcagtcg ggcggaattg
     2161 ccgatggcgt gcgcgccggt ggtacaggcg gaaaccatcc cgtagctcgg gccgcggtag
     2221 cctttgagaa tggtaacgtg tccggaaatc aggttgatca gcgaaccggg gataaagaaa
     2281 gggttgattt tgcgcgcgcc gccttcgatt acggctttgc cggtgacctc gatgctgggc
     2341 agtccgccga tgccggaacc gatgttcacg ccgatgcggt ctttgtcgag gttttccaca
     2401 tcgtccaagc ccgaatcggc gattgcctgc aatgcggcgg caatgccgta gtggatgaat
     2461 acgtccatcc ggcgcgcttc tttcgcgctg atgtattgtc cgatgtcaaa accgcgcact
     2521 tcgccggcga cacggctgtt gatgtcggat gcgtcaaagc gggtaatcgc gccgatgccg
     2581 cttttgccgg cgagcagggt gtcccaagcc tctgcgacag tgttgccgac aggggaaacc
     2641 tgacctaagc ctgtaatgac tactcttctc tgactcatga taacctcgct gttggttgtc
     2701 ggaatggggg catatgcggc tgtcgtgcag atgccgtctg taattttcgg caggggttca
     2761 aacagtttgc catataaggg aaaagcctct attgcgcggt gcagcagagg ctgttgtgtc
     2821 gggcgacgac cggttagccg ttgtgggcat tgatgtagtc gatagccagt tggacggtgg
     2881 tgattttttc ggcatcttcg tcggggattt cgcagccgaa tgcttcttcc aaagccataa
     2941 ccagctccac ggtgtccaaa gaatccgcgc ccaagtcgtc ttggaaagaa gattcgtttt
     3001 tcacgtcggc ttcgtttacg cccagttgtt cagcaacaat ttttttaact tgttgttcga
     3061 tgtttgacat atcagtcgtt cctttatgcc ttgcggcagg ttgtttaagg gaaataaatc
     3121 ggtggtattg taccgacttt taatagagtt ttctatctaa tgactattat atcaatattt
     3181 gccgatttgt acatttttgg gtgcggcggg ttttgtcgtt caagtttgac ctgtgtgccg
     3241 tatgtttgac gggatttcgg ttaaaatgac ggcatttcca tctgaagcag aaagccctgt
     3301 catgtatcca cttgcccgcc gtatcctgtt tgcactcgat gccgagaaag cccaccactt
     3361 cacgctcgac gcgctcaaca tggtttataa attgggtctg attcccgtaa ccggcaaccg
     3421 taccaaacct gtaaaactga tgggtttgga tttgcccaac cctgtcgggc ttgccgccgg
     3481 actcgataaa aacggcgaat atatagacgc attgggcgcg ctcggcttcg gtttcatcga
     3541 aatcggcacg gtaacgccca acccgcagcc cggcaacccg cagccgcgcc tcttccgcgt
     3601 tcccgaatat caaggcatca tcaaccgcat gggcttcaac aaccacggca tcgatgccat
     3661 gattcaaaac atcgaaaaaa gtaaattcag cggcgtattg ggcatcaaca tcggcaaaaa
     3721 cgctgtaaca ccgattgaaa acgctgccga tgattattta atctgcctcg aaaaagccta
     3781 cgcacacgca agttacatta ccgtcaatat ttcctcgccc aacactaaaa acctccgcgc
     3841 gctgcaaggc ggcgacgagt tgagcgcatt gcttgaggct ttgaaaaaca aacaggcaca
     3901 gcttgcctct gtacacggga aatacgtccc gctcgccgtc aaaatcgccc ccgatttgga
     3961 tgaagcacaa attgaagaca tcgcccacgt tgtcaaatcc gtcgaaatgg acggcatcat
     4021 cgctaccaat accaccatcg acaaatcaag cctcggtagc catccgctcg caggcgagca
     4081 gggcggtttg agcgggctgc ccgttcatga aaaaagtaat cgggtgttga agctgttggc
     4141 agaccacata gacggcaagc tgccgattat cggcgtaggc ggcattatgg aaggcgagga
     4201 tgcggcagag aaaatccgct tgggcgcgac cgccgtccaa gtgtacagcg gattgatata
     4261 caaaggtccg gcattggtca aagaatgttt gaaggctttg gcgcgatgac gcgtcccgcc
     4321 caaaatgccg tctaaacgca cgttttgccg ttcagacggc attttcattt cctttttccg
     4381 cctgccgccc cttgaaaatc ccttacgcgc cgccctgttt gaaataaggc aaacgcccgc
     4441 cgcgaaccgg acaatcttga tacgcagcca aagcaagtcc ggcggtaagt tgttaaatat
     4501 ataagcaatt gatacaaaaa ataattttat actgtggcgg acggcttggg aaaacgcaaa
     4561 atatgaacgg cgttgaaatt catgatgtgg aaaataagga tacggcggat aatcaaaaga
     4621 ttttatattt aaggatttga tatgtttaag gacgaactaa atgaatttat ccgattgata
     4681 tccgatccgg aaagtgaatt ggatgaatgg tatttgagtg attttaaaga tgaacatata
     4741 tgggaaatgc aaagttacga agcattttca tgtttgcggg aagcagtccc ctatttgttt
     4801 gcttatcccc gatacggata tgaactgttg gagataattt ccgcgttaaa agaaacatct
     4861 gatacgacag aattatttta tgagccgggt atcgtgcctt tgctgattga tttatataaa
     4921 gaagatagtt atctggtaaa tatggtcaaa cggatattca aatgatgaaa ggggcgcggg
     4981 cgtttgcttg gcggaaggca ggtaactcaa tttgaaggac gcggaagccg atacgttaaa
     5041 aaggatgaaa aatggaaaac gggcaacgga ataaccggtt tcctttggaa aaacgaattt
     5101 tttatctgga gcattcgggg cggtatctga tgatttgcgc cttatcggat tattcccaaa
     5161 acaaacatac tgtcgttatg gcaaatttca tctatccgga tgaaaaaacg gattggcgga
     5221 atttggatga tttattcaat gagttggttt tagaggaatt gcaggcttca tttatggatt
     5281 ggcatccgac tgttgaagag gcaatcagcc gtcatttgga agacttttcg taacaggcgt
     5341 caatcggaag gggcggaatg acggggcagg caatcggagt aaaaaatgaa ccttgattta
     5401 accgcgcaaa aagtccgtct ttcttggaag gatattctgt gggggtatgg gaataaatac
     5461 ttgggttggg ctgatgtggc agcttatgcc cgaaaaatga cgctttcaga tcatgatgaa
     5521 cgtgtgttca aactatcttt aatcaacaaa tccaatattc ttgaattaaa gcctgttctg
     5581 gaagatttgg cttcggaaat gagggattat tcccctaaaa attggctgta cgtcctctta
     5641 agcgatgtat tccatagaaa agaagaattt gaggatcctt tgggggaagt tgaaaaaatt
     5701 tatgcagatt ttgattatcc ggaagaaata gaatcatttg tcaggtatat gccgcccaaa
     5761 gacggttata ttccttctgc ccacacctat gaagaaaata ttgcccggtt atattctcac
     5821 tgggaacact atttgaacaa cggcggaggg cagggttaaa accggcaatc cgatgccgtc
     5881 tgaagcatta tccggccttc agacggcatt ttgttttccg acagtttata aactgtcgtt
     5941 gtttcttgac agaaacaacg accttatttg aaacgattgg aggacatgat tatgggtttt
     6001 tgggatagcg cggcaaattt ggcaaaagaa gcaggagaag gcgtaaagcg atttaacgag
     6061 agaagccgta aattgcagaa cgaatatcag caggaatcca gtgaaaaatt acgccgcgtc
     6121 gtaaacagcg aaggtttttt cggtaattcg gcacaagaaa aagccattgc ccgcaaagtt
     6181 ctgaaagaac gtggcgaagg ctaaacgagg gcagcgcatt tgccgctgtt tttttatcgg
     6241 taggtatccg tccgaatatc ggggtaaggt ttcagacggc atcgaaggtt gctatgatat
     6301 agtggcttga ctttaaaccg gtacggcatc ccctcgccct gtcctgattt gaagtgaatc
     6361 cgccatcatc ttcctgccat ccttccaaac ggaatccgaa atgtccgaca accgcctcga
     6421 caccgcccgc cgccattccc tgttccttgc ccgccagctc gacaacggca aactcaagcc
     6481 cgaaatattc ctgccgatgt tggacaaggt tttgaccgaa gcggatttcc aagcctttgc
     6541 cgactgggac aaaatccgtg cggaagaaaa cgaggaagaa ttggcgcggc agttgcgcgg
     6601 gttgcgccgt tatgtggtgt cgcagattat cgtgcgcgat atcaaccgca tcagcgattt
     6661 gaacgaagta acccgcacga ttacgctgtt tgccgatttt gccgtcaata ccgcgctgga
     6721 ttttgcctac gcctattatc gggacatgta cggcacgccg atcgggcgtt ataccaaatc
     6781 gccgcagcat ttgagcgtgg tggcgatggg caaggcgggc ggctatgagt tgaacgtgtc
     6841 ttccgacatc gatttgattt tcacctatcc cgaatcgggc gacaccgacg gcaggcgcga
     6901 acgggggaat caggagtttt ttaccaaagt cgggcagaaa ctgattgcgc tgttgaacga
     6961 catcaccgcc gacggacagg tgttccgcgt cgatatgcgg ctgcggccgg acggtgattc
     7021 gggcgcgttg gtattgagcg aaaccgcgct ggagcaatat ttgattacgc aggggcgcga
     7081 atgggaacgc tacgcgtggt gcaaaggccg cgtggttacg ccgtatccga acggaatcaa
     7141 atcgctggtg cgccctttcg tgttccgcaa atatttggat tacagcgcgt atgaggcgat
     7201 gcgtaagctg caccgccaaa tcagcagcga agtcagcaaa aaaggcatgg cggacaacat
     7261 caaactcggc gcgggcggca tccgcgaagt cgaatttatc gcccagattt tccagatgat
     7321 acgcggcggc caaatgcgtg cgctgcaact gaaaggcacg caggaaacgc tgaagaaact
     7381 tgccgaaacc gggatcatgc cgtctgaaaa cgtcgaaacc ctgcttgccg cctaccgatt
     7441 cctgcgcgat gtcgaacacc gcctgcaata ctgggatgac cagcaaaccc aaaccctgcc
     7501 ggcttcgccc gaacagcagc aactgcttgc cgaaagtatg ggtttcgaca gctacgccgc
     7561 tttttcagac gacctcaatg cccaccgcac caaagtcaat cagttgttca acgaaatttt
     7621 gagcgaaccc gaagagcaaa ccctagacac cagcgaatgg caatgggcat ggcaggaaaa
     7681 gcccgacgaa gaagagcggc aaggtcgtct gaaagaacac gggttcgatg ccgaaaccat
     7741 cgccgcaagg ctcgaccaaa tccgaaacgg tcataaatac cgccatctct ccgcacatgc
     7801 ccagctgcgt ttcgatgcgg ttgtgccgct gttcgtacag gcggcggcag agcaaagcaa
     7861 cccgaccgat acattgatgc ggctgttgga ttttctcgaa aacatcagcc gccgatccgc
     7921 ctatctcgcc ttcctcaacg aacatccgca aaccttggcg caactggcgc agattatggg
     7981 ccaaagttct tgggtggcgg cgtatctgaa caaatatccg attttgttgg acgaactcat
     8041 cagcgcgcag cttttggata ccgcgtttga ttggcaggca ctcgccgccg ccctttcaga
     8101 cggcatcgaa gcctgcggcg gcgatactga agcgcaaatg gacgccctgc gccgcttcca
     8161 gcacgcccaa gtcttccgtc tcgccgtcca agaccttgcc ggactgtgga cggtagaacc
     8221 cctctccgac caactctccg ccctcgccga caccatactc gccgccgccc tgccgtgcgc
     8281 atgggcggac atgccaaaaa aacaccgcga cacaccgcaa ttcgccatcg tcggctacgg
     8341 caaactcggc ggcaaagaac tcggctacgc ctccgacctc gacttggtct atctttatga
     8401 cgacccgcat cccgatgcag gcgacgtgta cagccgcctt gcccgccgcc tgactaactg
     8461 gctttccacc gccaccggcg caggcagcct ctacgaaacc gacctgcgcc tgcgccccaa
     8521 tggcgatgca ggtttcctcg cccacagcat cgccgccttt gaaaaatacc agcgcgaaaa
     8581 cgcatggacg tgggaacacc aatcccttac ccgcgcccgt tttatctgcg gtacgcccga
     8641 aattcagacg gccttcgacc gcatccgtac cgaaatgctg accgccgaac gcgaccaaac
     8701 ggttttagca ggcgaaatca tcgaaatgcg cgaaagaatg ttccccaccc atccgccggt
     8761 tgacagcaac gtcaaatacg cgcacggcgg cgtagtagac gtcgaattta tcgtccaata
     8821 tctgatactt gcccacgccc gaaaatatcc gcaactcctc gacaactacg gcaacatcgc
     8881 cctcttgaac atcgccgccg actgcggttt gattgataaa acgcttgccg gacaaagccg
     8941 caccgcctac cgcttctacc gccggcagca gcacaacacc aaactgcgcg acgcggcaaa
     9001 aaccgaagta accggcgaac tgttggcaca ttacggcaat gtcaggaaat tgtggcggga
     9061 agtgttcggc gaagaagcgg caaccgcctg aacaaaaaat gccgtctgaa gcctgacaat
     9121 ctgggtttca gacggtattt tcgtaccgtg ccgttttaag gttgcggcag agctaaagcg
     9181 atttatcggg aatggctgaa acccaaaaac cggattcctc tttcgcggga atgacgggat
     9241 ttcaggcttc tgtttttgtg ggaatgatgg gattttttat ccaagcaaaa atcaaaacaa
     9301 acaaataaga ac
//
LOCUS       DAXIEB010000080         9282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000080 DAXIEB010000000
VERSION     DAXIEB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.080"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(19..303)
                     /locus_tag="ACFOA3_001521"
     CDS             complement(19..303)
                     /locus_tag="ACFOA3_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898507.1"
                     /translation="MPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKMPSE
                     AVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
     gene            complement(309..1364)
                     /locus_tag="ACFOA3_001522"
     CDS             complement(309..1364)
                     /locus_tag="ACFOA3_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6898508.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1470..1952)
                     /locus_tag="ACFOA3_001523"
     CDS             complement(1470..1952)
                     /locus_tag="ACFOA3_001523"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6898509.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1999..3135)
                     /locus_tag="ACFOA3_001524"
     CDS             complement(1999..3135)
                     /locus_tag="ACFOA3_001524"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6898510.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3132..3677)
                     /locus_tag="ACFOA3_001525"
     CDS             complement(3132..3677)
                     /locus_tag="ACFOA3_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6898511.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTHQTGIRANT
                     QTDFSKFKGCLKDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3674..5149)
                     /gene="trpE"
                     /locus_tag="ACFOA3_001526"
     CDS             complement(3674..5149)
                     /gene="trpE"
                     /locus_tag="ACFOA3_001526"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6898512.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5260..5625)
                     /locus_tag="ACFOA3_001527"
     CDS             complement(5260..5625)
                     /locus_tag="ACFOA3_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898513.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5646..7556)
                     /locus_tag="ACFOA3_001528"
     CDS             complement(5646..7556)
                     /locus_tag="ACFOA3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6898514.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7575..8219)
                     /locus_tag="ACFOA3_001529"
     CDS             complement(7575..8219)
                     /locus_tag="ACFOA3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6898515.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8383..9201)
                     /gene="opc"
                     /locus_tag="ACFOA3_001530"
                     /gene_synonym="opcA"
     CDS             complement(8383..9201)
                     /gene="opc"
                     /locus_tag="ACFOA3_001530"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6898516.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2012 a         2041 c         2644 g         2585 t
ORIGIN      
        1 acgctgtact ggtttttgtt aatccactat aaatgttccg atacgaactg caaaatattg
       61 gttttgtttc tgacaggcaa aagcactgtt tatttggctg tcaaaaggat ggttaaggaa
      121 agttatgcgc ccctgaagcg ggccccagat aaggatggtt gcgccgacgg cttcagacgg
      181 cattttggcg gcggtgttgg gttttgtatc cggtttgccg ttgtgttttg tgatgatgat
      241 tttgggcgcg gtttttctgt tttgatgtgt gaaatgccgt ctgaaaggcg gttcagacgg
      301 catagcggtc atttttgtgc ggtcaggcgg tcgaatatgc cgccgtcggc gaagtaggtt
      361 ttcatgatgt tgtcccatcc gccgaatttt tcttcgggag agaatgagtc taagtctggg
      421 aagtcggctt tgtgtcttgc caatacttcg gggttgcggg ggcgcaggta gagtgaggcg
      481 gcgagttctt gcgccggttc gctccaaagg tattcgagat aggcgcgggc ggttttttgc
      541 gtgccttttt tcgcgacgac gctgttgacg acggcgacgg ggctttcggc ggaaatggtg
      601 tagctcggat agacgatttc aaactgtcct tgggtcagtt ttttgctgac gtagttggct
      661 tcgttttcaa aagtaatgag tacgtcgccg atgttgcgtt gtgtgaaggt ggtggtggcg
      721 gcgcgtccgc cgttttcaaa aacgggggtg tttttgagga tggatgcgac gagtttttgg
      781 gcttcctgtt cgttgccgtt ggtggttttc agaccgtaac cgtatgcgcc gaggaaggcg
      841 tagcgtccgt tgcccgaggt tttgggattg gcgatgacga tgttaacgcc gtctttggca
      901 aggtcgttcc aatcgcggat ctgtttgggg ttgttttttc ggacaaggaa aaccatagtg
      961 ctggtgtagg gcgcggcgtg gtcggggagg gcttgttgcc agcctttttc taccagtcct
     1021 tttttttcga gcaggtcgat gtcggaggat tggttcatgg ttacgacatc ggcttgaagg
     1081 ccgttggcta cggataatgc ctgtttgctg gagccgccgt gggactgttg gatgctgacg
     1141 gatgtgccgg ggtgttcgga ttggtatgtt ttgataaata aggggttgta ttctttgtaa
     1201 aaatcccgtg ccacatcgta tgaggcgttg agcagggtaa tgttttttcc gtcggattcg
     1261 gtattggccg gggcattttg tccggacgga tggtttgaat cggctgcggg gctgcaggcg
     1321 gtgagcaggg ctgcggtata gagtgccggt gcgtaggttt tcatatgctt gtcctgtcgg
     1381 ttggtagatg gggcaacttt atacggctgt ctgcgcttgt ggaaataatg tttgatttga
     1441 agattatcag ttttggttat aaggacggat cagaggtgtt tccgcatcag ttcgcatttg
     1501 attttgatgc tggggtcaag ctgcaatact gccgaaccga gcgattcgta gcggttgaga
     1561 aggtaaaacg ggactgagtt gagcgatgcg tagagtttca gaaagctcaa accggatttg
     1621 tgggcgatgg tttccgcctg atgaagtagg gcagtgccca gtccgaggtt gtggaacagg
     1681 gggtggacgt aaagtgcatc gagttgtgct tcttggcagt cgatttggaa aaatccctgt
     1741 atgttgcctt tgtattcggc aacccaaagt gctttgtcgg gatcggaaat ggtcggcagg
     1801 tagctttctg tgtttaacaa gccttcccat acttttaggg cgtgttcgtt gtagctgagg
     1861 atgcaggtgt attggacgga gtgcaggtgg actttgaaga tgtctttgca gtcttgcacg
     1921 gtggcggggc gtaacagtgt cagcaggttc atggcagtat gtcggcggct tcagacggca
     1981 tctgtgccgt tggtcggatt atagggactg atgcagtttt tttgcttctt gaaatgcggt
     2041 gtccgaatcg gtggttaaaa cggtaaagtg tcccattttc cgccctttgt gcgcggtttt
     2101 tttgccgtaa aggtgcaggt gcgcgtccgg ccggctttgc aggggaaacc aatccggttc
     2161 gccgccgtct tcctgccaaa cgtcgcccaa aatattcgcc atacagcaag gcgataataa
     2221 tttggtgtcg gcaggcggca ggttgcacat aatgcgtacc tgttgctgga attggtctgc
     2281 cgcgcaggca tcgacggtat ggtggccgga gttgtgcgga cgcggcgcga tttcgttgac
     2341 gaccaattca tgtgtgtcac cgacaacaaa catttctacc gccaatacgc cgacataatc
     2401 caattcgtcc gccaagcgtt gcgccatctg ccgcgcctgt tgctgcacgt cggcactcag
     2461 tcgcgcgggg acgatggaat aagccaagat gccgttttcg tggatgtttt cggcaggatc
     2521 gaaagtttgc acgttgtcat tgttcagacg gcatacgata acggaaattt cgccgcgcaa
     2581 gtccaccatt ttttccaaaa cgcaatccac gccgcggtgt tcggcaaacg cggctttgag
     2641 ttcatccacc gtttttacgc ggatttggcc tttgccgtca tagcccaatg tagcggtttt
     2701 caggatgccg ggcagaaatt gtatgctttc ttcagtgatg tcttcggctt tgcaaattgc
     2761 ttgatacggc gcggtttgca ggcctgcttt gcgtatccat gacttttcct gaatgcggtt
     2821 ttgcgcgatg gcaacgcagt cgccgctggg ggaaacattg gtatgtttgg cgagaaaacg
     2881 catcgcatcg gcattgacgt tttcgaactc ggtcgtaaca gccgcacatt ttgccaattc
     2941 ttccaaagcg gtttggttgt caaacggcgc acacaaatgg cggtcggcaa attccgctgc
     3001 cggcgcattc gggttgggat cgagtacggt tactttgtag cccatggttt tggcagcaac
     3061 agtaaacatt ctgcctaatt gtccgccgcc aagaatgccg agcatggcgg gcggagaaag
     3121 agatatgttt ttcatgctga ctcttcaaat tgtacaagtt gatagctata actaattctt
     3181 gacggatgtc ttgtatcgct ggaattacca gtttcagaaa tacagaatac tttttccata
     3241 aatttttctg cttttagaaa ttccagtatt ctgttttttt catccttata agcaccgcgg
     3301 tctgtacccc atgcaagaat aatcatatcg gcatctttca aacatccttt aaatttggaa
     3361 aaatcggttt gggtgtttgc cctaattcct gtttgatgtg tagagtaact ggaaaaaata
     3421 tttaacattt tgaagttggt aaaaccgtac atatccaaga aacgtgcaag ttgggtaagg
     3481 gttttgtcgc ttctttcatc atttgcttta cttggattaa ttcctatagc gacagctgag
     3541 aaatttttag gattttctgt gttgccgctc catctaaccg ttaggatttc tcgatttttt
     3601 tcattatctg tatagagacc gtctttggta gtcggtctga gtgtttggcg aagctcataa
     3661 agtttttcat aagtcattta tccaaccctt cctgcaccat ctgcgcggcg cgtataacgg
     3721 ctcgggcttt gttttgtgtt tcctgccatt cggaggccgg atcggagtcg gcaaccacgc
     3781 ccgcgccgct ttggacgtat agcgtgttgt tttttactac ggcggtgcgg atggcgattg
     3841 ccaaatccat gtcgttgttg aaaccccata cgccgacggc accgccgtag atgccgcgtt
     3901 tgctcggttc gacttcttcg atgatttcca tggcgcggac tttgggtgcg ccggagagtg
     3961 tgccggcagg gaaggtagcg gcgaggatgt ccatgttggt catgccgtct ttcagacggc
     4021 cttcgacgtt ggaaacgatg tgcattacat gggagtattt ttcaatcacc attttgtcgg
     4081 taactttgac ttcgccggtt ttactgatgc ggccgacgtc gttgcgtcct aagtcaatca
     4141 acatgacgtg ttcggcgatt tctttggcat cgcttaacaa atcttgttcg ttggcaaggt
     4201 cttcggcggg ggttttgccg cgcaggcgcg tgccggcgat ggggcggacg atgacatcgt
     4261 cgcgttcgcg gcggacgagg atttcgggcg aggagccgac gatgtggaaa tcgccgaaat
     4321 cgtagtaaaa gagataaggc gaagggttga gcgtacgcag ggcgcggtag agggcgagcg
     4381 ggctgtcggt gaattccatg ctcatgcgct ggctggggac aacctgcatg cagtcgcctg
     4441 cgaagatgta gtctttgatt ttgttaacgc aggctttgaa cggctcttcg ccgaattcgc
     4501 tgacggcttc ggtgtgtttg ctgccgagcg agagcgggat ggcgcagctt tggcgcaact
     4561 gggtgcggat gtcttcgagg cgttcgcggg cgcgttcgta gccgtcgggc tgcgacggat
     4621 cggcgtaaac gacgagatgg attttgccgc tcaaattgtc gatcaccgcc aattcttgcg
     4681 acagcatcag caagatgtcg ggcgtgccga gcgggtcggc tttggtggtg tttttcaggc
     4741 ggtgggcgaa gtgttcaaaa ttgtagatgg tttcgtaacc gaagtagccg accagtccgc
     4801 cggtaaagcg cggcaggctt gggatttcgg gtgttttgaa gcggttgtgg aaggcttcga
     4861 taaagggcag ggggttgccg tcgtgttgct cgacaatttc gccgttttga taaacatcga
     4921 cgtgtttgcc gctggctttg agatagtggc tgcaaggcag gccgataaaa gaatagcggc
     4981 cgaaacgttc gccaccgaca acggattcga gcaggtaggt ataggggcgg ttggcgagtt
     5041 tgagatagag ggaaagcggc gtatccaaat cggcaaggag ttcttgcacg agcgggatgc
     5101 ggttgtagcc ttgggcggct tgggcttggt attcttgttt gctgatcatt tctgctttcc
     5161 caaagggcgg tttcggacgg cgcggcaacg ggcgcgagta tagcatttta tcggaattgt
     5221 tgacagtctg accggagatg ccgtctgaaa aggacgggct tacggctttg ttttgcgctg
     5281 ccggtattgt ttccatttaa acagataacc tgcggttacg ggaatcaggg ctaagatggt
     5341 tttgaacgtc catgatgcat accaaggtaa agaaccgggt gcgaccgatt gattcagata
     5401 cggccaccaa caggcagtca ggcagccgag cagcagcggc cagatatgcc ggctgaggtt
     5461 tttttgccaa aggaggaatg atgcggcaga ccaaagcagg gtaagggcag tgatttttaa
     5521 cacggtataa gcatccgctc caaacagggc ggcgagaaaa tacgtaccgg caatccagag
     5581 gacggcaagt atcaggacaa tgatttcttc aggtcgtttg aacatttttt tcttcctgtt
     5641 tgatttcaga cggcattgcc gttctgtttg gtttccagca gctcccagcg ttccagcttt
     5701 tccaaaagca gcatttcgat ttcttcggcg cggctttgca gtgcgccggc tttttcgtaa
     5761 tctttgaaga tttcgggatc ggaaagctgc gcgttgattt ctgcctgttc ggcttccaag
     5821 gcggcgattt cgtcgggcag ggcgtcgagt tcgcgctgtt ctttgtagga cagtttgacc
     5881 gtgcggttgg ctttgggttt ttctttcacc ggttcggcat cggctgtttt gggtgcggat
     5941 tccgtctgaa tcttcgcttc gcgtgatttt gcgtcgatgt agtcctgata gccgccgata
     6001 tattctttta accttccatt tccttcaaaa ataatgcttt gggtaatcac gttatcgagg
     6061 aacatacggt cgtgtgagac aaggaatacc gtgccttgat aatcgcgcag caaatcttca
     6121 agcagctctt gggtatcgat atccaagtcg ttggtcggtt cgtccaaaat caaaatatta
     6181 gcaggacggg taaagagttt tgccagcaaa aggcggttgc gttcgccgcc ggagagcgat
     6241 gaaacgggac tttgtgcgcg ggctggatgg aataagaaat cttccagata gctcattacg
     6301 tgttttttct tgccgccgac ttcgacgtaa tcgttgcctt gaccgagcgt gtaaaacacg
     6361 gtgtcgtttt cgttcaacgt gctgcggaat tggtcgaaat aggcaacttc ctgcttactg
     6421 ccgatacgga ttctgccgta ggtcggctgc aattcgccca aaatcagctt gaggaaggtg
     6481 gttttgccga taccgtttgg gccgattaag ccgattttgt cgccacgctg caagatggcg
     6541 gagaatttgt ccatgatgac tttgtcgcca taggtaaacg aggcatgttc cagttcggca
     6601 atgattttgc cgcttttctc gccgctgtcg agcttgaaat tgacctgccc ttgtacgttg
     6661 cggcgttcgg cacgctggcg gcgcagttct tccaaacggc gcacgcggcc ttcgttgcgg
     6721 gtacggcgcg cttcgatgcc tttgcgtatc cacgcttctt cctgcgcgtg gaatttgtcg
     6781 aagaggcggt tgtgttccgc ttcgactgcc aactcttgcg cttttttctc gctatatttg
     6841 gagaacgagc cgggatagga acgcaaaata ccgcgatcga gttcgacaat ccgcgtggcg
     6901 atattgtcca aaaaacggcg gtcgtgggta atcacaacca ggctgccttc aaacgctttg
     6961 agcaggtttt ccaaccaaat aatcgcgtcg atgtccaaat ggttggtcgg ttcatcgagc
     7021 agcaatacgt cgggcttctg cacccaagcc tgcgccaagg cgacgcgctt tttctgaccg
     7081 ccggagaggt tgccgatttt ttcgttttcc ggcaaaccga gttcgcccaa agtctgcttc
     7141 accgccgcat ccaacttcca gccgtccttc gcttcgatct caagttgcaa ttcgttgagc
     7201 tctttcaata aggcctcact cgaaccattt tccaactcat ggctgacatg atgataacgg
     7261 cgcaataaat cgcgaatttc gcccaaacct tcggcaacag tatcaaatac ggttgcttcc
     7321 ttatcaaaaa aggattcctg cggtacataa acgattttga ggttgttttg gacaataatc
     7381 tgcccatcat caagcttttg cacgccggcg aggattttta aaaacgaaga cttacccgca
     7441 ccattacgac cgattaagcc gattttttcg cctctgtcga gttggaagga agttttgtcg
     7501 agcagggcga cgtggccgac ggcaaaggaa gcgttttcta cagataatat attcatgata
     7561 caaattctca acagttaccg tttggatttt accgcaagtt tggcgcgggc aatttcaacc
     7621 gcacccggca ggacggaaac aataatgatg ccgcccatca ccaagcccag attgtttttt
     7681 acgacgggga agttggcaaa gaaatagccc gcgtaagaaa acaggataac ccacaacaag
     7741 ccaccgatga tgttgtagcg gataaatttg gcatagtgca ttttccccat accggcgacg
     7801 aagggggcga aggtgcggac gatgggcata aaacgggcaa tgatgatggt tttgccgccg
     7861 tgtttttcgt aaaaacggtg ggttttatcg agatattcac gtcggaagat tttagaatcg
     7921 ggattggcga acagcctgcc gccgaaatat ttgccgacgg taaaattgag cgcgtcgccg
     7981 agtatggcgg caaggcttaa taatgcaacc atcaaatgaa tatccatacc gcccagcgcg
     8041 gcaataccgc cggcggcaaa cagcagcgaa tcgccgggca gtaagggcgt aacaatcagg
     8101 ccggtttcgc aaaaaacaat caaaaacaga atcgcataaa tccacacacc gtattgcgcc
     8161 gacagcgcga gcaggtgttg gtcgatatgg aggatgaagt cgatggcgga ggcaagcacg
     8221 gcgcgttcct aaaaaaaaca aaccgcgtat tttaaccgat tggaaaaatg ccgtctgaaa
     8281 ttttcagacg gcatcggcta ttcaaattca tttcacgtaa aaaccgcaaa ccaaaatagt
     8341 ttgcggtttg gcatttaaag tgacaatgat gatttcaaat catcagaatt ttatgccgac
     8401 gcgcaagccg tattcacgaa tactggtttt cgggatggtg agcgatacgt cgccactctt
     8461 ggttgttaca ctaaactcgc cggattcttt gtaagtgcgt tgtttgtaga acggccccgc
     8521 ctcgatgctg gcggattcgc ccagtttttt accaatattt gcacccaatc caaagcccca
     8581 gccgttggtt ttttgattga tgtctttttt gagattggta acgccggtgt ttaatttata
     8641 gcgggaattg aggtcaaatt tcacttcaga ccaagggttg atataccagc cgttacccag
     8701 ttgggaaagc aaatccgcgt gaactttggc taaccacgac tgacggctgc tgtgaagcgt
     8761 atgcttggtg gttttaatgc tgtcttttga agattcaaaa cccaagccgg cacccacacg
     8821 gaaatttaaa gaatcactta acgtttgggt gtaggtgtag cctgtgtaaa gatcgatacg
     8881 gttttcagga acgccggtgg gcagttttac atttttagtc ttacccagct tgttctcatc
     8941 tgtttccaaa ttaataatat tttttttgct gcgcccgaaa ccggcttcca agcggatgcc
     9001 ttggttggca tcaaaaggaa tatcagcacg tacgccgatg tgtttggcag ctttgtgttt
     9061 ttctttcagg aaagcacgag ttgaagaaat ggaagagagg tcggtgtgga cggtaaactc
     9121 attagcggtt tgaagctctt gtgcagcggc ggcagtaccg gtcagggcaa tcatggcaca
     9181 tgtaaaaact gtttttttca tagttaaaat ctccaaaatt tggattgtag tcggatatgg
     9241 taacataacg taaataatcg ttacgcttac aattatattc tt
//
LOCUS       DAXIEB010000081         9077 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000081 DAXIEB010000000
VERSION     DAXIEB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.081"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(134..469)
                     /locus_tag="ACFOA3_001531"
     CDS             complement(134..469)
                     /locus_tag="ACFOA3_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6898517.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            598..1503
                     /gene="mtrA"
                     /locus_tag="ACFOA3_001532"
     CDS             598..1503
                     /gene="mtrA"
                     /locus_tag="ACFOA3_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC6898518.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1565..2053)
                     /locus_tag="ACFOA3_001533"
     CDS             complement(1565..2053)
                     /locus_tag="ACFOA3_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6898519.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2200..3102
                     /locus_tag="ACFOA3_001534"
     CDS             2200..3102
                     /locus_tag="ACFOA3_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6898520.1"
                     /translation="MDTAKKDILGSGWMLVAAVCFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSLSSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3143..4273)
                     /locus_tag="ACFOA3_001535"
     CDS             complement(3143..4273)
                     /locus_tag="ACFOA3_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6898521.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPSAENPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7102
                     /gene="alaS"
                     /locus_tag="ACFOA3_001536"
     CDS             4478..7102
                     /gene="alaS"
                     /locus_tag="ACFOA3_001536"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6898522.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTTKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7164..7895
                     /locus_tag="ACFOA3_001537"
     CDS             7164..7895
                     /locus_tag="ACFOA3_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898523.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8202..>9077
                     /locus_tag="ACFOA3_001538"
     CDS             8202..>9077
                     /locus_tag="ACFOA3_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC6898524.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRN"
BASE COUNT         2305 a         2399 c         2190 g         2183 t
ORIGIN      
        1 tcacgcttac cccttgtttc tcaaatgccg tctgaaaaaa acggctgtat atgttgtctg
       61 caaaatatcg ggaagcataa cagacaaaat gccgtctgaa atattttcag acggcatttc
      121 aatccgattc ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg gcgtaagtgt
      181 aagcggcacc ggcattcagg gcgatggcgg ttgccaatgc gccggcgatt tcgctgtcgg
      241 tcgcaccggc tttggcggcg gcggcggcgt gaacactgat gcagctctca caacgtgtgg
      301 taatggcaac ggcgatggca atcagttcgc gcgttttggc atcaagtgct tcggcggcgg
      361 cggcctgttc caatgcgccg taggcctgca gcattttagg atgcgcctta cccagctcgc
      421 cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt ttcttttcct
      481 ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg cgtattattg
      541 caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa aagatttatg
      601 gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga tgtgcagtgc
      661 cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg attggtacac
      721 attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc gcgtccggtc
      781 agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag ccacgacgga
      841 aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac ggtcaagcag
      901 tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga cacccacgcc
      961 gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc gagtttacag
     1021 tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac ggtttccgtg
     1081 gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct cgaacaggat
     1141 aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt gggacattta
     1201 atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat ggtggcggct
     1261 gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg actcagcccg
     1321 cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa aaaaaacccg
     1381 gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca cttcggcaag
     1441 gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg cgggcaaaaa
     1501 taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc atttgcgtac
     1561 cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta aatgcggggt
     1621 tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag gcattctgga
     1681 tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc gccgaacgca
     1741 ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt tttctgaagt
     1801 tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc tgatagccca
     1861 agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag gcaaggcgga
     1921 aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg atttccgtca
     1981 atgtgggcac ataaatagac tggcagccgg aaagaactgc cgcaatggaa agagggataa
     2041 ggtatttttt catgccccca ttataatcaa gtttgccttg agaaaacaaa ttgttcggca
     2101 agaaaaataa aatttcggca tcagaagcag gcaaaaacac attccacaag ccttgccgca
     2161 aggtttacaa tccgaccgtc cttatcgcaa cgaccgttta tggataccgc aaaaaaagac
     2221 attttaggat cgggctggat gctggtggcg gcggtctgct tcaccattat gaacgtattg
     2281 attaaagagg catcggcaaa atttgccctc ggcagcggcg aattggtctt ttggcgcatg
     2341 ctgttttcaa ccgttgcgct cggggctgcc gccgtattgc gtcgggacac cttccgcacg
     2401 ccccattgga aaaaccactt aaaccgcagt atggtcggga cgggggcgat gctgctgctg
     2461 ttctacgcgg taacgcatct gcctttggcc accggcgtta ccctgagtta cacctcgtcg
     2521 atttttttgg cggtattttc cttcctgatt ttgaaagaac ggatttccgt ttacacgcag
     2581 gcggtgctgc tccttggttt tgccggcgtg gtattgctgc ttaatccctc gttccgcagc
     2641 ggtcaggaaa cggcggcact cgccgggctg gcgggcggcg cgatgtccgg ctgggcgtat
     2701 ttgaaagtgc gcgaactgtc tttggcgggc gaacccggct ggcgcgtcgt gttttacctt
     2761 tccgtgacag gtgtggcgat gtcgtcggtt tgggcgacgc tgaccggctg gcacaccctg
     2821 tccctctcat cggcggttta tctgtcgggc atcggcgtat ccgcgctgat tgcccaactg
     2881 tcgatgacgc gcgcctacaa agtcggcgac aaattcacgg ttgcctcgct ttcctatatg
     2941 accgtcgttt tttccgccct gtctgccgca ttttttctgg gcgaagagct tttctggcag
     3001 gaaatactcg gtatgtgcat catcatcctc agcggtattt tgagcagcat ccgccccact
     3061 gccttcaaac agcggctgca atccctgttc cgccaaagat aaaaaatgcc gtccgaacat
     3121 ccttcagacg gcatatcggg ctttatttcc ccgccttcac atcctgccac tggcgcacca
     3181 taaacttcaa tgccgccggc tggataggca ccatgataaa gctgtttttc aaatcctcct
     3241 cggttgggaa aatcgtattg tcgtttttaa attcgtcttc catcagctca cgcgccggct
     3301 tgctcgaagg cgcataagta acgaaattgc cgtttttcgc cgacacttcc ggatcgagga
     3361 agtcgttgat gtatttgtgc gcgttggcga cgtttttcgc atctttcgga atcacgaaag
     3421 aatccaccca aatccccacg ccctctttgg gcatcatcac gcggattttt tccttgccgc
     3481 ccgcttcttc ggcacggcgt ttggcgatgt tcaaatcgcc gccgaaaccg attgttacgc
     3541 aggtatcgcc gcgcgccaaa tcatcgataa agccggacga agtaaagcgt ttgatattgg
     3601 ggcggttttt cttgagtagg gcggttgcct ccctgatgtc ttccgtattg ctgctgttcg
     3661 ggtttttacc caaatagttc aacaccatag gatagatttc cgccgcgctg tccaaatagc
     3721 tgatgccgca ttgcttgagt ttggacgtgt attcggggtc gaacaccaaa tcccactggt
     3781 tgtccggcag cttgtccgta cccaaagcct ttttcacgcg ttcggtattg atggcgaagg
     3841 tatttgtccc ccaataaaac ggcacggcgt attcgtggcc gggatcgacc ccgtccatca
     3901 gcctcatcat ttcggggttg aggtgtttat aattgggaat cagcgactta tcgattttct
     3961 gatacgcacc tgccttaatc tgcctgccca caaacgcatt ggacggcgcg acaatgtcgt
     4021 aaccggactt gcctgtcagc accttgcttt ccagcgtttc atcgctgtcg tacacatcat
     4081 aagtaacctt gatgccgttt ttcttttcaa aatcggcaac ggtttccgga tcgacatatt
     4141 ccgaccagtt gtaaattttc aatacgtttt ggttttccgc cggtgccgga ttttcggcag
     4201 atggtttgtc cgaaccgccg cacgctgcaa gcagcaaagc agtcaggacg gccaggggca
     4261 gatgtttggt cattatcatt ccttgcatat cgggttggag aaagcggcca ttatagccga
     4321 tattggcaac agggcttcag acggcattca aaatcccgcc acactcttcc gaaaaccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccaat ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgccggcggc aaacacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgcgt ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag ccccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaatccgct
     5161 gcccaagccg tccgtcgata ccggcatggg cttggaacgc atggccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgcg ccgttcagta tggaagaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctca ttcctgattg cagacggcgt cttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc attatccgcc gcgccgtgcg ccacggttac aaactgggtc aaagcaaacc
     5461 gttcttccac aaactggtgg ccgatttggt caaagagatg ggcggtgcct accctgagtt
     5521 gaaagagaaa caagcacaaa tcgaagaagc gttgaaaaac gaagaaagcc gttttgccca
     5581 aacgctggaa actggtatgg ctttgttgga aaacgcgctg gccaaaggcg gcaaaacact
     5641 cgacggcgaa atcatcttca aactctacga cacctacggt ttcccatacg atttgaccgc
     5701 cgacatctgc cgcgaacgca atatcgaact ggacgaagca ggcttcgagc gcgaaatgga
     5761 agcccaacgc gcacgcgcac gcgccgccca aagcttcaaa gccaacgccc aactgcctta
     5821 tgacggtcaa gacaccgagt ttaaaggtta tagcgaacgc caaaccgaat ccaaagtcct
     5881 cgccctctac aaagacggcg agcaagtcga cgaattgaac gaaggcgaca gcggcgcagt
     5941 cgtcatcgac tttaccccgt tctatgcaga atccggcggc caagtcggcg acgtcggcta
     6001 tatcttctca ggcgaaaacc gctttgaagt acgcgatacc caaaaaatca aagcggccgt
     6061 attcggccaa ttcggtgtac aaacctcagg ccgtctgaaa gtcggcgaca gcgttaccac
     6121 caaagtagat gacgaaatcc gcaatgccaa tatgcgcaac cacagcgcga cccacctgat
     6181 gcacaaagcc ctgcgcgatg tattgggcag acacgtcgaa caaaaaggct ctttggttac
     6241 cgccgaatcc acccgtttcg acatttccca tccccaagcg gtaactgccg aagaaatcgc
     6301 cgaagtcgaa cgccgcgtca acgaagccgt tttggcgaac gttgccgtca acgccgccat
     6361 tatgagcatg gaagacgcgc aaaaaaccgg cgccatgatg ctcttcggcg aaaaatatgg
     6421 cgacgaagta cgcgtattgc aaatgggcgg tttctctacc gaattgtgcg gcggcacgca
     6481 cgtttcacgc accggcgaca tcggcctctt caaaatcatc agcgaaggcg gtattgccgc
     6541 aggcgtgcgc cgtatcgaag ccatcaccgg cctgaacgca ctcaaatggg cgcaagagca
     6601 agagcgtttg gtgaaagaca tcattgctga aaccaaagcc caaaccgaaa aagacgtact
     6661 ggcgaaaatc caagcaggcg cggcacacgc caaagccttg gaaaaagaat tggcacgcgc
     6721 caaagccgaa ctcgccgtcc acgcaggcgc aaaactcttg gacgacgcaa aagacttggg
     6781 cgcagccaaa ctcgttgctg cccaaatcga agccgacgca gccgccctgc gcgaaatcgt
     6841 taccgattta accggtaaat ccgacaacgc cgtgattctt ttagcggcag taaacgacgg
     6901 caaagtctcc ctgtgcgccg gcgtatccaa accgttgacc ggcaaagtga aagcaggcga
     6961 tctggttaaa tttgcagccg aacaagtcgg cggcaaaggc ggcggccgtc ccgacttggc
     7021 gcaggccggc ggcacggatg ccggcaaatt gcccgaaatg ctggtaagcg tggaaagctg
     7081 gctttgtcag aaattaagtt aaatttaaat cttaataagc catttgagtc ttgttcaaat
     7141 ggcttatatt ttggattgtt agaatgaata ctaaaacaga gttacaaaaa cttttagaag
     7201 aggatatatc aactttaaca gaaacactaa tttgtgcaga tgctcttcct ccaagatatg
     7261 tacgtagcat tgcaactcct attgtccgtc gatggttaat tgacaaacaa ttaaatattc
     7321 tcgcaaaaga aataggttta actatagaat tgcccatcct tgatacaagt ttggtatttg
     7381 aaaaattgtc aaccttagag aataaagtta atttttatat ggctggtgga gtttatttag
     7441 gcggagaatt tattagctct atatatcatt catcacaaga attttctggt gaaccaataa
     7501 tctatgctga accaaatata attttatgtc ctgctgaaaa atttttaact ttaaaacgag
     7561 ttttccataa tggaaatatt tttaatatga atcagataat tactttttta tccaataaac
     7621 aaggaggagt acattttgat aaaaattacg ataaatataa aacttggcaa gttgctatag
     7681 aaaaagcagc aaatttttta aaactcggta atccatataa tgaagataaa ttatctctta
     7741 gtgaagaaca tgatactatt ttagtagttt taccattaga aaaaggatat gaatggaatt
     7801 gtcttgagat agaagtacta tccgctgctc aatctctagc aaatatctat tgcaacaaag
     7861 ttcgcctcat agatggtcat gtttggaagg aataaaagaa aaagcatagt tggtaatttt
     7921 tagttacgta gggtgggctt cagcccaccg ttttttcatt tttgattgtt gattggtggg
     7981 ctaaagccca ccctacggtt actaaaatcc cgtcattacc tcgcaggcgg gaatccggtt
     8041 tgttcggttt cggtttttta gggacagtgc cgcaacatta aatttccaga ttcccacttt
     8101 cgcacaaata acagcgcaaa tagggcaaag ataaggatat gggttttttt ctttgaattt
     8161 actatacaat tcgcccgtcc ccgttataga aacccaagcc gatgaaaacc ctgctccaac
     8221 tccaaaccgc cgcacaaaac ttcgccgcct actacaaaga ccaaaccgac gaacgccgcg
     8281 agaaagacac cttctggaac gaatttttcg ccattttcgg catcgaccgc aaaaacgtcg
     8341 cccacttcga ataccccgtc aaagaccctg ccgacaacac ccaattcgtc gatatatttt
     8401 gggagggcat cttccttgcc gaacacaaat ccgccaacaa aaacctgacc aaggccaaag
     8461 agcaggcgga gcgttatttg caggaaatcg ggcgcaccaa gccctctgcg ctgcccgaat
     8521 attacgccgt cagcgatttt gcccatttcc acctttatcg ccgcgtacct gaagaaggcg
     8581 tggaaaacca atggcagttc cctttggaag cattgcccga atacatcacg cgcggcgttt
     8641 tcgacttcat gttcggcatc gaagccaaag tccgccaaat tcaagaagaa gccgatattc
     8701 aagcggcggc ggccatcggc aggctgcacg acgcgctcaa agaagaaggc atttacgaag
     8761 aacacgagct gcgcctcttc atcacgcgcc tgcttttcct cttttttgcc gacgacagcg
     8821 ccgttttcca gcgcaactat ctctttcaag actttttaga aagctgcaaa gaaaccgaca
     8881 cgctcggcga caagctcaac caactctttg aattcctcaa cacacccgac caaaagcgca
     8941 gcaagaccca aagcgaaaaa tttaaaggtt tcgaatacgt caacggcggc ctgtttaaag
     9001 aacgcctgcg caccttcgac ttcaccgcca agcagcaccg cgccttaatc gactgcggca
     9061 atttcgactg gcgcaac
//
LOCUS       DAXIEB010000082         8892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000082 DAXIEB010000000
VERSION     DAXIEB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.082"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            142..576
                     /locus_tag="ACFOA3_001539"
     CDS             142..576
                     /locus_tag="ACFOA3_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6898525.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(625..1968)
                     /locus_tag="ACFOA3_001540"
     CDS             complement(625..1968)
                     /locus_tag="ACFOA3_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6898526.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1973..2626)
                     /locus_tag="ACFOA3_001541"
     CDS             complement(1973..2626)
                     /locus_tag="ACFOA3_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6898527.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2777..3067
                     /gene="gatC"
                     /locus_tag="ACFOA3_001542"
     CDS             2777..3067
                     /gene="gatC"
                     /locus_tag="ACFOA3_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6898528.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3130..4575
                     /gene="gatA"
                     /locus_tag="ACFOA3_001543"
     CDS             3130..4575
                     /gene="gatA"
                     /locus_tag="ACFOA3_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6898529.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPIGV
                     QLIGNYFSEAKILGAAHQIQLNSDWHGKRPE"
     gene            4572..5504
                     /locus_tag="ACFOA3_001544"
     CDS             4572..5504
                     /locus_tag="ACFOA3_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6898530.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPLRRTA"
     gene            5547..6977
                     /gene="gatB"
                     /locus_tag="ACFOA3_001545"
     CDS             5547..6977
                     /gene="gatB"
                     /locus_tag="ACFOA3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6898531.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAVRFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7084..8094
                     /locus_tag="ACFOA3_001546"
     CDS             7084..8094
                     /locus_tag="ACFOA3_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6898532.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDNGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRVVHFYWGARHQDDLYALEEAQGFACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8224..8856)
                     /gene="pdxH"
                     /locus_tag="ACFOA3_001547"
     CDS             complement(8224..8856)
                     /gene="pdxH"
                     /locus_tag="ACFOA3_001547"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6898533.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
BASE COUNT         2290 a         2720 c         2166 g         1716 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacgg
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcgttatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc ttccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaactca aatgcgaaaa acaaggatga tttgattcgg
      541 aaatacaata acgatgtgaa caaatactgc cgttgagcag cctcatcgaa aaccccatgc
      601 cgtctgaaac ggcacggggt ttgtttattt ctgccagtag gttttgacgt tgacgaactc
      661 gtacagcccg aattcggaca attcgcgccc gtaaccggaa tctttgactc cgccgaaagg
      721 caggcgcaaa tcgctgctgg tatggcggtt gataaacacc gatcccgcct gtattttttc
      781 ggcaaaccgc caagcgcgtt cggtatcggc ggtataaatg caggcaccta gcccgaacgg
      841 ggaatcattg gcaagggcga tggcatgttc ttcgttttcg gcgcgcaaaa tcagggcggc
      901 cggcccgaat acttcttctc tccagacgcg gcaggcagga tttaccctgt ctaaaaccgt
      961 cgcgggataa aaccagcctc gcccttgtgg gatttttccg ccggtcaggc ataccgcgcc
     1021 gtttgaaacg gcatcttcaa cctgcccgtg aaccctgtcc cgcaaatctt cgcggtgcag
     1081 cggtgcaagc gtagtatcgg gatgtttggg gtcgcccatt ttcaatttgg cgcattcggc
     1141 aagaaacagc gtgataaaac gatcggctgc ggcttcggtt acgatgatgc gtttggcggc
     1201 gttgcacgat tgccccgcat cgcggaaacg ggaataacag gcttctgcgg cggcgcgttc
     1261 caaatcggca tcgggcatca cgataaaggc gttgctgccg ccgagttcca agacggtttt
     1321 cttgaggttt gcgcccgcgt gtgccgcaag gatgcgcccc gtatgcgtcg agccggtaaa
     1381 cgccattgca tcggtatctt caaccgcctt gagcgtgccc gcctcatcca gccacacgcc
     1441 tgccaaagga atgccgtctg aagccaaatc gaacagtgcc tgactgacgc gtgccacgct
     1501 gggcgcgggt ttgacggcgc acgcgttgcc cgcgcacata gcgggaacgg cgaaacgcaa
     1561 tacctgccag acgggatagt tccaaggcat gacggcaaac accacgccca aaggctcgaa
     1621 gcgcacctga ctcaaactcg cctgcgtcgc gatggttttg tgggcaagca gttcgggggc
     1681 aaggcgggcg taatagcgta tcagttcgat agacttgccg atttccgcac ggcattcgtg
     1741 caagcagcgt ccgacttcct cacacaccat ttccgcaaaa cgctctttct ccgcctccaa
     1801 acggtcggca aatttttgca ggcgcgcggc acgttcggtt acgcccagtt gcgcgaacgc
     1861 cccgccgcgc attttcaaat ccgccagccg ccgttcaaac tccgcataat cttgagctgg
     1921 acggcggtaa agcgtttcgc ccgtaaatac attgacactg tgaaacatcg aatcaacctg
     1981 ccagttgcgg gaatatcgtt ttcagtcccg acacaataat ctccaccgat accgccgcca
     2041 gcatcatacc cataatgcgg tttaaaatcg tcagccccgt cgcgcccagc aggcggctga
     2101 ccttcccggc aacgattaaa atggcataac aaatcgcact gaccaccaaa ccggccgcga
     2161 taatcaacgc gatgtcgccg tatgttttag ccgccgaagc gtaaataatc acggtcgaaa
     2221 taccgcccgg gccgatggtg atcggtatgg cgatgggcac gacggcaatc gctccggcat
     2281 tgcgggcggg gcgcgcctgc cccgtttccg gctgcgcgcc gagattctgc ttggcgggat
     2341 tgtcgttgcc gttcatcata gaaatggcaa tcagcaacac caaaatcccg ccgccgacct
     2401 gaaacgaacc gacgctgatg cccaaaacct tcagcagcgt accgccgatc agcgcaaata
     2461 ccgcaatcac ggcaaacacg gcaacggcgg ccgtccgcgc gaccttcctg cgctccttcg
     2521 tgctgtgccc gttggtcagg tcaaggtaaa gcgacaacgc gctaaacgga ttaatcagca
     2581 ccaaaaaagc cacaatcagc ttgccgattt ccatgcccaa tcccattatt tccccctcct
     2641 tcaaacccgt gcggcaggca tccgatgctg caaattgccg ccgcaacgga tttttccgtt
     2701 ataattaaaa attcaagcaa tacgccccat catacccgaa cgacggtatc tttaccatca
     2761 gacaaggatg cttttcatgg cactgacact tgccgacgta gacaaaatcg cccgactctc
     2821 ccgactgcac ctgactgcgg aagaaaaaga aaaatcgctt caagaattaa acgacatttt
     2881 cactatggtc gaacagatgc aaaccattaa cacagacggc atcgaaccga tggcgcaccc
     2941 gcacgaggcc gccctgcgcc tgcgcgaaga cgaagtaacc gaaaccgacc gcgccgccga
     3001 atatcaggcg gtcgcaccgg aagtacgcga ccgcctgtac atcgtaccgc aagttatcga
     3061 agaataatcc gaatacgctt cagacggcat cagcaatacc gcccgaagcc ctttaaggat
     3121 ggaagattta tgacccaata cacattgaaa caggcgggca gcctgttgca gtccaaacag
     3181 atttccgccg tcgaactggc aagcgcatac cttgccgcca tcgccgaaaa aaatcccgcc
     3241 ctcaacggct atatcaccat cgaccaagat aaaacccttg cagaagcccg tgccgccgac
     3301 gaacgtatcg cgcagggcaa cgcctccgcg cttaccggcg tacccgtcgc ctacaaggat
     3361 attttctgcc aaaccggctg gcgcagcgcg tgcgcttcca aaatgctcga caacttcatc
     3421 tccccctaca ccgccaccgt cgtccaaaac ctgctcgacg aaggtatggt aacgctcggc
     3481 cgcaccaata tggatgagtt cgctatgggt tcgaccaatg aaaactcatt ctacggcgcg
     3541 gccaaaaatc cgtggaatcc ggaacacgtc cccggcggtt cgtcaggcgg ttccgccgcc
     3601 gtcgttgccg ctcgccttgc ccctgccgcg ctcggttcgg ataccggcgg ctctatccgc
     3661 cagcctgcat cgcactgcgg cattaccggc atcaaaccca catacggcac ggtttcccgc
     3721 ttcggtatgg tcgcctacgc ctccagcttc gatcaagccg gcccgatggc gcaaactgcc
     3781 gaagactgcg cgattctgtt aaacgcgatg gcaggtttcg acccgaaaga ctccaccagc
     3841 ctcgagcgcg aaaaagaaga ctacacccgc gatttgaacc aaccgctcaa aggtttgaaa
     3901 atcggcctgc ccaaagaata tttcggcgaa ggcaacagcg ccgatgttct gacggcattg
     3961 caaaacacca ttgatttgct gaaagcccaa ggcgcggaat tgattgaagt ttccctgccg
     4021 caaaccaagc tgtccatccc cgcctactac gtcctcgcct ccgcagaagc cggcaccaac
     4081 ctttcacgtt acgacggcgt gcgctacgga caccgtgccg cccaattcgg cgatttggaa
     4141 gaaatgtacg gcaaaacccg cgccgaaggt ttcggcagcg aagtcaaacg ccgcatcatg
     4201 attggcactt atgtactgtc gcacggctac tacgatgcct actatctcaa agcccaaaaa
     4261 ctgcgccgcc tcgttgccga tgattttcag acggcatttg cacggtgcga cctcatcctc
     4321 gcgccgaccg caccctccgc cgcacccaaa atcggagcgg atgcttcgcc ggttgaaacc
     4381 tacttgagcg atatctacac catcgccgtc aacctcgccg gactgcccgc attgaccctg
     4441 cccgcaggtt tcagcggcgg cggactgccc atcggtgtac agcttatcgg caactatttc
     4501 tccgaagcca aaatcctcgg tgcggcgcat caaatccaac tcaacagcga ttggcacggc
     4561 aaacgacccg aatgaagcag aaccgcacct ttaccttccc cgattttcgc accgtttaca
     4621 gctatgcgcc tttatatcgg ctgcaacatt taaaatacac attgcgaaaa tttttcggaa
     4681 aaaaagaaat ttacgccttc gagcagtttg tcaacgcctc ccctatccgt caggggctgt
     4741 tcctccactg cccgcaaaat gcctatccgc tgctgcgcga atttgttgac aggcgtttta
     4801 actgcaaacg ccgtttagat gcgatgacgg cagattttct catggcggaa aaactgttcg
     4861 gcacagacat cctgcaccaa atggaagact accgcttcca tctggtcttg gcgcaccttt
     4921 cagacggcat cagcttgtgg ctcaaccgca acgacaactg cgtcgaagaa ggcgcgtggt
     4981 ctttatcttt gcgcgacgaa gcaggcaacc ggctgtatat ggcgactttc gcctttgtcg
     5041 gcacacacct gctgacagcc tccgtacaag ggccggcggg tgaagaagcc aaagacaccg
     5101 tccgccgcat aaccaaacaa ctccacggct tgcgtcccca acaactgatg gtaaccgccc
     5161 tgcaatattt cgccgccgta ctcggcttgg acggcgcaat gggcattgca caaaaacatc
     5221 aggtcaaact gcgctggaaa cttaaaaagc gtgtcaaaat gaattacgac gcattctggc
     5281 aggaatacgg cgcaagtttg gaacgggacg gctactggca tctcccccaa acccccgccc
     5341 gcaaagacct tgccgacatc gaaagcaaaa agcgttcgat gtaccgcaag cgttatgaaa
     5401 tgctggacaa tatggttgca gagatgaaag acagtctgaa aacagaagca cgcggcattt
     5461 cagacggcat ccaaacggaa aaaccgctcc gccggacagc ctgacgcgaa gactatcgaa
     5521 ttgatatttt agagaaagaa gctcttatga cctgggaaac cgtaatcggc ttggaaatcc
     5581 acgtccaatt gaacaccaaa tccaaaatct tcagcggcgc atcgaccgca ttcggcgcag
     5641 aacccaacgc gcacgccggc gtagtggaat gtgctttgcc gggcgtactg ccggtaatga
     5701 accgtgaagt cgttgaaaaa gccatcaaat taggcttggc tttggatgcg aaaatcaacc
     5761 ggaaaaacgt gttcgaccgc aaaaactact tctatcccga cttaccaaaa ggttatcaaa
     5821 tcagccagtt ggacttaccg attgtcgaac acggcaaatt ggaaatcgta gtcggcgacg
     5881 atgtgaaaac catcaacgta acccgtgcgc acatggaaga agacgcaggc aagtccgtgc
     5941 atgaaggctt gaacggcgcg accggtatcg acctgaaccg cgccggcacg ccgctgttgg
     6001 aagtcgtatc cgaacccgaa atgcgctccg ccgccgaagc cgttgcctac gccaaagcct
     6061 tgcacagctt ggtaacctgg ctgaacattt gcgacggcaa tatggcggaa ggttcgttcc
     6121 gcgtcgatgc caacgtatcc gttcgcccga aaggtcaagc ggaattcggc acgcgccgcg
     6181 agattaaaaa cctcaattcc ttccgtttct tggagcaggc gattaattac gaagcagaag
     6241 cgcaaatcga gattttggaa gacggcggca aagtacagca ggcaacgatg ctgtttgatc
     6301 ccgaaaaagg cgaaacccgc gtaatgcgcc tgaaagaaga cgcgcaagac taccgctact
     6361 tccccgaccc cgatttgctg cccgtcatca tttcagacgc tcaaatgcaa aaagccaaag
     6421 cagaaatgcc cgagctgccg aaagaaatgg cagtgcgttt cgtggcggac tacggcgtgt
     6481 ccgaatacga cgcgcgcctg ctcaccgcaa gccgcgcaca ggccgcctat tttgaagaag
     6541 ccgccaaaga gagcggacaa ggcaagccga ctgccaactg gatgaacggc gaactcgccg
     6601 ccgcactgaa caaagaaggc atggaacttg ccgacagccc gattaccgct ccgcgcctcg
     6661 ccgcgctggt aggcaaaatc gccgacggca cattaagcag caaattagcg aaaaaagcct
     6721 ttgaagccat gtgggcagaa cccgaagcca ctattgccga aatcatcgaa aaacacggtt
     6781 tgcaacagat gaccgatacc ggcgcggttg aagcgatggt ggacgaagtg ctagcaaaca
     6841 acgccaaagc cgtggaacag tttaaatccg gcaacgaaaa agccctgaat gcgattgtgg
     6901 gacaagtgat gaaggccagc aaaggcaaag cgaatcccgc gcaggtgcag gagttgatta
     6961 aagctaaact ggcttaatcc gttatcacac aggtcgtctg aaagcaaagt tccaacgaag
     7021 gtaaaacagg aaataagctt tcagacggcc ttttaactta atctgctcaa accataccaa
     7081 gacatgaacc acaccgttac cctgcccgac caaaccacct ttgccgccaa cgacggtgaa
     7141 accgttttgg ccgctgccgc ccgacaaaac ctcaacctgc cccattcctg caaaagcggt
     7201 gtctgcggac aatgcaaagc cgaactggtc agcggcgata ttcaaatgga cggacactcg
     7261 gaacaggctt tatccgaagc agaaaaagcg caaggcaaga ttttgatgtg ctgcaccact
     7321 gcgcaaagcg atatcagcat caacatcccc ggctacaaag ccgatgccct acccgtccgc
     7381 accctgcccg cacgcatcga aagtattatt ttcaaacacg atgtcgccct cctgaaactt
     7441 gccctgccca aagccccgcc gtttgccttc tacgctgggc aatacattga tttactgctg
     7501 ccaggcaacg tcagccgcag ctactccatc gccaattcgc ccgaccaaga aggcattttg
     7561 gaactacaca tccgcaggcg cgaaaacggt gtctgctcgg aaatgatttt cggcagcgaa
     7621 cccaaagtca aagaaaaagg catcgtccgc gttaaaggcc cgctcggttc gtttacctta
     7681 caggaagaca acggcaagcc cgtcatcctg ctggcaaccg gcacaggcta cgcccccatc
     7741 cgcagcatcc tgctcgacct tatccgccaa aacagcagcc gcgtcgtcca tttctactgg
     7801 ggcgcgcgtc atcaggatga tttgtatgcc ctcgaagaag cacaaggatt cgcatgccgt
     7861 ctgaaaaacg cctgcttcac ccccgtattg tcccgcccca gagagtgctg gcaaggaaga
     7921 aaaggccacg tacaagacat cgcggcacaa gaccaccccg acctgtcgga atacgaagta
     7981 tttgcctgcg gctctccggc catgaccgaa caagcaaaaa atctgtttgt gcaacagcat
     8041 aagctgccgg aaaacttatt tttctccgac gcattcacgc cgtccgcatc ataattcccc
     8101 ggtataaaaa agattcaggc tttccgttcg gaacacaaaa aacttcccgt ccgtattttc
     8161 cccgtgaaaa aatgccgtct gaaacccgat tcctgttttc agacggcata tgttttttcc
     8221 tgttcaaggc gacagccgct cgcgtatcca gccgccatcc agcaaacggt attggatgcg
     8281 gtcgtgcagc ctgctcggcc tgccctgcca aaactcaatc aaatcgggaa tcacaatata
     8341 gccgccccaa tgcggcggac gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc
     8401 ctttgccacc aataccgcct tgttcggaat cacctcgctc tgcgcacttg cccacgcacc
     8461 caaacggctc tgatacgggc ggctctcaaa atattcgtcc gacaacttct ccgccagcct
     8521 ttcaacacgc ccttccacgc gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc
     8581 cgcaaatgga tgagcatcca gcgaacgccc cttgcggctg tgataattcg taaagaaaac
     8641 aaaaccttca gaattaactt ccttcagcag caccatacgg ctgttaggcc tgccgcgtcc
     8701 gtcgaccgcc gccacattga ccgccgtcgg ctcgttgacc tgtgcgcgta ccgcctcgtc
     8761 caaccaccgc tcgaactgct cgatcggatt gtcggcgcaa tcgccttccg acaattcccg
     8821 tttgctgtaa tcttcccgaa tattgtgcaa atccatttac cgctcctctt aatagtggat
     8881 taacaaaaac ca
//
LOCUS       DAXIEB010000083         8880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000083 DAXIEB010000000
VERSION     DAXIEB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.083"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            314..787
                     /locus_tag="ACFOA3_001548"
     CDS             314..787
                     /locus_tag="ACFOA3_001548"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6898534.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKK
                     YIDEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDF
                     FEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(996..2381)
                     /gene="pntB"
                     /locus_tag="ACFOA3_001549"
     CDS             complement(996..2381)
                     /gene="pntB"
                     /locus_tag="ACFOA3_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6898535.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKSANSVIITPGYGMAVAQAQYPVAEITELLHKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(2447..2830)
                     /locus_tag="ACFOA3_001550"
     CDS             complement(2447..2830)
                     /locus_tag="ACFOA3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6898536.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKTGGFRFSDNHHPRDFLARTQ
                     GAAARAHAAQQNSFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2834..4375)
                     /locus_tag="ACFOA3_001551"
     CDS             complement(2834..4375)
                     /locus_tag="ACFOA3_001551"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6898537.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKATVWACPLIYKVNAPSEDELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSDDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFKKG"
     gene            4665..5498
                     /gene="serB"
                     /locus_tag="ACFOA3_001552"
     CDS             4665..5498
                     /gene="serB"
                     /locus_tag="ACFOA3_001552"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6898538.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMNFDELGLIVSDMDSTLITIECVDEIAEGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPSAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGVGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            5562..6785
                     /locus_tag="ACFOA3_001553"
     CDS             5562..6785
                     /locus_tag="ACFOA3_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6898539.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGVGWCLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGAAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFVLIGAIAVWFP
                     EILGNGKAGNQLTFSGLTGWQYALELAAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPAMSSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6946..8526
                     /gene="purH"
                     /locus_tag="ACFOA3_001554"
     CDS             6946..8526
                     /gene="purH"
                     /locus_tag="ACFOA3_001554"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6898540.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         2004 a         2377 c         2505 g         1994 t
ORIGIN      
        1 gcgggaatga caactcatta gttacctaaa acttaaaaaa cggaaacctt tacgccgtca
       61 ttcccacgaa agtgggaatc cgggaactta acgttacagt gatttatcgg aaacggctga
      121 aaccgaatga attggattcc cgcctgcgcg ggaatgacga atccatccgt acggaaacct
      181 gcaccacgtc attcctacga acctacattc cgtcattccc acgaaagtgg gaatccagga
      241 cgaaaaatct ccagaaaccg ttttacccga taagtttccg caccgacagg tctggattcc
      301 cgcctgcacg ggaatgacga tgcaggtatt tctgacgatt cccggctatg atgttgaggc
      361 agaaatcgaa aaattcgttt ggatggatgc tgtgatttgg cagatgccga gctggtggat
      421 gcacgagcct tggacagtga aaaaatacat agacgaagta ttaaccgctg gacacggcaa
      481 actctaccaa agcgacggca gacacagcgt caatccgact gagggctacg gcacaggcgg
      541 cttgttgcaa ggcaaaaaac atatgctttc actgacttgg aatgcgccga ttgaggcgtt
      601 tacccgcgaa ggcgatttct ttgaaggcaa aggcgttgat gttttgtata tgcacttcca
      661 caaagccaac gagtttttgg gtatgacccg cctgccgaca ttcttatgta acgatgtggt
      721 taaaaatccg caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt
      781 cggctaatta aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg
      841 tgtcagtttc agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg
      901 caaaaaaaat cactgcaaac cttcataaac ggggtttgca gtgatttttt caaatcaaac
      961 agattgaaaa cctgcgccga attgttcaga cggcattatt ttttcagttc ggacagaatg
     1021 tcatctacgg ttttcttcgc atctccgaaa cacattacgc tgttttcgtt gaagaacagc
     1081 gggttttgta cacctgcgta gccggtattc atcgagcgtt tgaagacgac gacttctttt
     1141 gccttccata cttccaacac gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg
     1201 gcaggattga cggtatcgtt cgcaccgatg accaagacca catcggtttc ggggaaatcg
     1261 tcgttgattt cgtccatttc caacacgatg tcgtagggga ctttggcttc ggcaagcagt
     1321 acgttcatat gaccgggcag gcggccggcg acggggtgga tgccgaagcg tacttctgtg
     1381 ccgtttttat gcaaaagctc ggtgatttcg gcaacggggt attgcgcctg tgcgactgcc
     1441 atgccgtagc ccggggtgat gatgacgctg tttgcgcttt tcagcatttc ggcaacatcg
     1501 gcagctttga cttctcggta ttcccctatc tcttggctgc cggaagatga tgtgccgctg
     1561 tcgctgccga aaccgccggc aattaccgag acaaacgagc ggttcatggc tttgcacata
     1621 atgtaggaca gaatcgcgcc gcttgagcca accagcgcgc cggtaacgat gagcaggtcg
     1681 ttggagagca taaagcctgc cgctgcggcc gcccagccgg agtaggagtt gagcatggac
     1741 acgaccacgg gcatatctgc gccgccgatg gaggcaacca agtgccagcc gaatgcgagg
     1801 gcaatcaggg tcataatcag caggatgaag ccgctgccgt caatgccgac gaatacgagc
     1861 agcaacacaa acgatacgcc aagtgccagt gcgttgagct tgtgtttggc gggcagttgc
     1921 agcgggctgc tgctgatttt gccgttgagc ttgccgaatg cgaccagcga gccggtaaag
     1981 gttaccgcgc cgatgaaaat gcccaaatag acttcgacca gatggatggt gtgcatatcg
     2041 tgcgaaacgt tgcccggcgc gatatagctg ttgaagccga ccaaaaccgc cgctaggccg
     2101 acgaagctgt gcagcagggc aatcagttcg ggcatttcgg tcatttccac ctttttggct
     2161 ttgtagatgc cgattgccgc gccgatgagc atggcgatga tgatccagcc cagtccgtgg
     2221 gtattgtcgg aaaaaacagt tacaaaaagg gcgaccgcca taccggcgat accggaatag
     2281 cagccctgtt tggcggtttc ctgtttggac agccccgcca gtgagaagat gaataaaatt
     2341 gcggcaacga tatacgccgc tgttacgagt cctgaagaca tggaaattct ccgattttcg
     2401 atgatttgtt tttaatgccg tctgaactgt tcagacggca tctgtttcaa gcagccgcga
     2461 caaacagccc gacggtgcag gcaaatccgc ccgcccacat cagcgagcgc aatgctgctt
     2521 tgtctgcgat gtagcaccag ataaaggcga ggcggaacag gatgaacagg ccggcaagcg
     2581 tgttgacggt tgcttgtccg gcattgccgg ttgcgtgtgc cgtcaaaacg gcggctgcaa
     2641 atggtgcaaa tgcttcaaaa ctgttttgct gcgcggcatg ggcacgggca gcggcacctt
     2701 gggtacgcgc cagaaaatca cggggatgat gattgtcgct gaagcggaat ccgccggttt
     2761 ttttggcata cgctgcacaa aaaagcggca ataagcaggc aatcaggatg caccaatagg
     2821 cgaaagtcat ggcttaccct ttcttaaaca tattcaacat gcggcgcgtt acgaagaagc
     2881 cgccgaagat attgatgctg gcaatcagga tggcgatgaa ggcaagcagg gtaacgaaac
     2941 cgttgccttg gctgatttgc agcagcgcgc cgacgacgat gatgccggag atggcgttgg
     3001 ttaccgacat cagcggtgtg tgcagcgagt ggctgacgtt ccaaacgacg tagtacccga
     3061 tgacgcaggc gaggacgaac acgataaagt ggttcaggaa tgctgcgggt gcgaccgcgc
     3121 cgacccacag caccaatacg gcggcgatga cggcgggcgc gagttttttc cacagggaaa
     3181 caggtttcgg ctcgggcttg gcggcaggcg cggctttttc agacggcgtt tgctgcggct
     3241 gggcggaaac ttgaatcggc ggaggcggga aggtgatttc gccgtcgtgg gtaacggtca
     3301 tgttgcggat aatcacgtct tcgaagtcca acgtgatttc gccgtctttg ttcgggctta
     3361 acagcttggt caggttgacc aagttggtgg cgtaaagctg ggaagactgt ccggcaaggc
     3421 ggtttgccat gtcggtgtag ccgatgattt tcacgccgtt gccggttacg gacaattcgc
     3481 ccgggcgggt gagttcgcag ttgccgcccg tcgccgccgc caaatcgacg atgacggagc
     3541 cggatttcat gctttccacc atttctttgg taatcagctt gggcgcgggt ttgcccggaa
     3601 tggcggcggt ggtgatgatg atgtccactt ctttcgcctg ctcggcaaag agcttcatct
     3661 cggctgcgat aaattcgtcg ctcatcactt tggcgtagcc gtcgtcgctg ccgcccgatt
     3721 cttgtgggaa gtcgagtttc aggaacttgc cgcccatcga ttcgatttgt tccgccactt
     3781 ccaagcgggt atcgaacgcg cgtaccactg cgccgagcga gtttgccgta ccgatcgccg
     3841 ccaaacctgc cacacctgca ccaatcacca aaacctgcgc gggcggcact ttgccggcgg
     3901 cggtaatttg accggtgaag aaacggccga aggcgttggc ggcttcaatt acggcgcggt
     3961 agccgctgat gtttgccatc gaagacaaag cgtccaaagc ctgcgcgcgc gaaatacggg
     4021 gcaccatgtc cattgccagc gcgttgactt tcttggcgcg caaggcttcg accaaagcct
     4081 cgttttggcg cggccacagg aagctgacga tggtctgtcc ttctttgagc agcggcagct
     4141 cgtcttcgga cggcgcgtta accttataaa tcaaagggca tgcccaaacc gttgctttgt
     4201 cggcaacggt cgcgcctgct gtttggtaag cggcatcgtc caaacttgcc gccaaacctg
     4261 caccgctttc gacaacggtt tcaaagccca gtttgcccag cagggcaacg gtggcgggcg
     4321 tgcaggcgac gcgggtttcg ccggataatg actcacgtgg gataccgatt ttcatctctg
     4381 aatccttttt cgggttgttt atatgtatcg tgggttaaat ttaaatcgtg gcggggcgga
     4441 gcaacgccgt accggtttaa aaccgattca cttcaaatgt taatatattt tagataatcc
     4501 ccttataacg aattttcatc aggctggcaa tagttgcggc attttcccgt gttgtccgac
     4561 acatactgcc gtttgcgttt gaagtcattg tccgattgca gtccctcccg ccgtaacatt
     4621 ctttgccgtt tcactatata atccgcattt tttgagccgc cgttatgccg cacgccctcg
     4681 tcctccaatt tccctccgcc gaagccctgc cttccgactt cccctcccgc ctgcccgaac
     4741 ctgattgcgc cgatgaaaag cgtatgcgtt ttatcgttga agaagggttt tctttaagcg
     4801 gggcggacgc ggcgttgctt ggcagccgtc aaatcgacca cgccgtgttg ccagatatga
     4861 attttgacga actcggttta attgtcagcg atatggattc gacactgatt accatcgaat
     4921 gcgtcgatga aattgcggaa ggcgtgggtt taaaaaacaa agtagcggaa attaccgagc
     4981 gttcgatgcg cggcgaactc gatttcgaac agtctttacg cagccgcgtc gcgctgttgg
     5041 cgggattgga cgaacagatt ttggcggacg tttatgaaaa cgttttgaag ctctcgccca
     5101 gtgcggaatt tttgttggac gaatgcaaaa ggcacaatgt gaaattcctg ctggtgtccg
     5161 gcgggttcac gttttttacc gaaaggctgc aacaacgcct cggcttcgaa taccaacacg
     5221 ccaatatttt ggaaattgaa aacggcaggc tgaccggacg tttgaaaggc agaattatcg
     5281 acgcgcaggc aaaggcggat ttattgcgcg aataccgcag ccgcctcgga ttgcagccgc
     5341 atcaggtgtt ggcgatgggc gacggtgcga acgatattcc gatgctcaaa gaagcgggcg
     5401 tgggcgtggc ttaccgcgcc aaaccgaaag cgcaggcagc cgccgatgcc tgtatcaatt
     5461 tcggcggttt ggagcgtgta cgcggcctgt tcaggtaggc ggataggaaa cggatgccgt
     5521 ctgaaaggtg ttcagacggc atttgaacgg caggaacgac agtgggacgc agaaaacttt
     5581 ggtttgccct ggcagcagcg ggcgttatcg gcggtttggt cggcattgtg ctgacggaac
     5641 tgatgcactt catacagcat acggcatacg gttatggcgc ggacggcgtg tacacttcgt
     5701 tccgcgaagg tgtggcacag gcttccggta tgcggcgcgt tgccgtgctg acgctgtgcg
     5761 gcgcggtcgc aggagtcggc tggtgcttgc tgaaagcctt gggcaagccg caaatcgaaa
     5821 tcaaagccgc cttgaagcag ccgttgcagg ggctgccgtt tctgacgacg gtttttcatg
     5881 ttctgctgca aatcataacg gtcggactcg gttcgccgct cggacgggaa gtcgcgccgc
     5941 gtgaaatgac tgccgcgttt gcttttgccg gcggcaggcg gttgggtttg gatgaagacg
     6001 aaatgcgcct cttgattgcc tgtgcgtccg gcgcgggttt ggcagccgtg tataatgtgc
     6061 cgctcgcctc cacacttttc atcctcgaag ccatgctggg cgtgtggacg cagcaggcgg
     6121 ttgccgccgc gttgctgact tccgtcattg ccaccgccgt ggcgcgtatc ggcttaggcg
     6181 acgtacagca gtatcatccc gccaaccttg ccgtaaatac ttcattgctt tggttttccg
     6241 ccgtcatcgg cccgatattg ggcgcagccg ccgtcttttt ccagcgtacc gcccaaaagt
     6301 tcccctttat caagcgcgac aacatcaaaa ttattccctt ggcagtctgc atgtttgtgc
     6361 tcatcggcgc gattgccgtg tggtttcccg aaattttggg caacggcaaa gcaggcaacc
     6421 aactgacttt tagtggtttg accggttggc aatacgcttt ggaacttgca gccgtcaaat
     6481 ggctggtcgt cttgatggcg ctggcggcag gcgcatacgg cggcctgatt acgccgtcta
     6541 tgatgctcgg cagcaccatt tcttttgcag ccgccgccgc gtggaacagc gttttccccg
     6601 caatgtcgtc tgaaagcgcg gcagtcgtcg gagcggcggt gtttctcggc gtgtcgctca
     6661 aaatgccgct gaccgccatt gtgttcgtat tggaactgac ctacgcgccg ctgtccctgc
     6721 tgctgccttt gtgcatcggc atggcggggg cattgggaac atcgggaaaa atgggcttca
     6781 aatgaatgcc gacaaatgcc gtctgaaagg ttttcagacg gcatttcttt gtaaattgtc
     6841 agcaatatct gcaaaaacct actgccaaaa aactagaaat agcggataat accgccccga
     6901 aaaagttccc ctttccccat ccaactgtca gaaaaggaag ccccaatgtc cgtcatcaaa
     6961 cgcgccctga tcagcctgtc cgacaaggca ggcgcggtcg aatttgccca aactctgcac
     7021 aagctcggtg tcgaaatcct ctccaccggc ggcacggcga aactgcttgc cgatgcgggc
     7081 gttcccgtga ttgaagttgc cgactatacc ggcttccccg aaatgctcga tggccgcgtg
     7141 aaaaccctgc atccgaaaat ccacggcggc atcctcggtc gtcgcgattt gggcgaacac
     7201 gtcgccaaga tggaagaaca cggtatcggc aacatcgacc tcgtgtgcgt caacctttac
     7261 cccttcgccg ccaccatcgc caaaccaaac tgcacactgg aagacgcgat tgaaaacatc
     7321 gacatcggcg gcccgactat ggtgcgttct gccgcgaaaa actggaaaca cgtcgccatc
     7381 gttaccgata ccgccgattt ccctgccatc gccgccgaac tcgaagccaa caacggcgca
     7441 ttgagcgaca aaacccgttt caacctctca cgcaaagcat tcagccatac cgcccaatac
     7501 gacggcatga tttccaacta cctgacctcg ctttcagacg acgtcttgag cggagagccc
     7561 caaatcggcg aattcccgag ccagttcaac caaagctgga ttaaagtgca agatatgcgc
     7621 tacggcgaga atccgcacca acgcgccgcg ttctaccgcg atgtgtaccc cgccgcaggc
     7681 agcctttccg cctacaaaca gctccaaggt aaagaattgt cgtacaacaa catcgccgat
     7741 gccgatgccg catgggaagc cgtcaaatcc tttgacgcgc ccgcctgcgt gattgtgaag
     7801 cacgccaatc cgtgcggcgt cgccgttgca gccgatacct tgaccgccta caaactcgcc
     7861 tacgccaccg acaccaccag cgcgttcggc ggcatcatcg ccttcaaccg cgaagtggac
     7921 ggcgaaaccg tcaaacagat tacagacaac cagtttatgg aagtcctgat ggcgccgaag
     7981 ttcaccgccg aagccctcga aatcgccgcc gccaagaaaa acgtgcgcgt gttggaagtg
     8041 cctttggaag caggcgcgaa ccgctttgaa ctcaaacgcg tcggcggcgg attgttggta
     8101 caaacgcccg acatccaccg catcagccgc gccgatttga aagtcgtctc caaacgccaa
     8161 ccgaccgaac aggaatggaa cgatttgctg ttcgtttgga acgtcgcaaa atacgtcaaa
     8221 tccaacgcca tcgtcttcgg caaaggcggt caaacctacg gcatcggcgc aggccaaatg
     8281 agccgcgtgg acagtacccg catcgccgcc cgcaaagcgc aggacgcagg cttggactta
     8341 aacggcgcat gtgccgcctc tgatgctttc ttccccttcc gcgacggcgt ggacgtgatt
     8401 gccgagcagg gcatcaaagc catcatccat ccggcaggtt cgatgcgcga tcaggaagtc
     8461 ttcgacgcag cggacgaaca cggcatcgcc atggtcgtaa ccgacgtgcg ccacttccgc
     8521 cattaacgca gatgaacaag gcaatgccgt ctgaagggct ttcagacggc attttgcgct
     8581 attttgtgaa ggtagggatg acggttcggg tattcctgac agggtggatt ttcaaggtgt
     8641 tgtatagggt gtaggaggat tcgtaaaagg tgggatgcag ggtgtgcttc agcccgctgc
     8701 atcaaaaatt tttggagaat cggcgggagt cggcggtttt ggtttcggcg gggacggtgg
     8761 aaatgggtaa cattgacgga atcgacggaa gcggtgggct tcagcccacc gcttgtatat
     8821 tggaatcacc gtatcatagc gccacccgcg cccacccaag gcacacaacc gttgcgtagc
//
LOCUS       DAXIEB010000084         8636 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000084 DAXIEB010000000
VERSION     DAXIEB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.084"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(175..867)
                     /locus_tag="ACFOA3_001555"
     CDS             complement(175..867)
                     /locus_tag="ACFOA3_001555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6898541.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="ACFOA3_001556"
     CDS             complement(975..2168)
                     /locus_tag="ACFOA3_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6898542.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFOA3_001557"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFOA3_001557"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6898543.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="ACFOA3_001558"
     CDS             complement(3021..3950)
                     /locus_tag="ACFOA3_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6898544.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFOA3_001559"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFOA3_001559"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6898545.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFOA3_001560"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFOA3_001560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6898546.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLIAGNHAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAQ
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGVRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYRTQKSDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7218..7730
                     /locus_tag="ACFOA3_001561"
     CDS             7218..7730
                     /locus_tag="ACFOA3_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6898547.1"
                     /translation="MSQVFKDFDLSSVWKTNSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIALIAVQNGGMFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGKNGEPKVVFVELAPDFETFIRSLR
                     HEDEFIDEEI"
     gene            complement(7920..8135)
                     /locus_tag="ACFOA3_001562"
     CDS             complement(7920..8135)
                     /locus_tag="ACFOA3_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898548.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTTG"
     gene            complement(8259..8618)
                     /locus_tag="ACFOA3_001563"
     CDS             complement(8259..8618)
                     /locus_tag="ACFOA3_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6898549.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2145 a         2413 c         2154 g         1924 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg tgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc agaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccagttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accagcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcacttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaagcggac aagccagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgttt gggcgcgatg attcccatca atgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctgatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaat gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg cgcccgatcc
     5281 gcacacgcgc cgcatgattg agtgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggttgat ttcgccgaac tgatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccaa cactaccaaa accgcttcaa ccacattctc gttgacgagt tccaagacac
     5461 caacaaactg caatacgctt ggctgaaact gattgccggc aaccacgccg ccgtctttgc
     5521 cgtcggcgac gacgaccaaa gcatctaccg gttccgtggc gcgaacgtcg gcaatatgac
     5581 cgcgctgatg gaagagttcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaat atccttgccg ccgccaacgc cgtgattgaa aacaacgacg aacgcctcgg
     5701 caaaaacctg cgtaccgacg ccgaagcagg cgacaaaatc cgctactact ccgcctttac
     5761 cgaccttgaa gaagcccaat tcatcgtgga cgaaaccaaa gcccttgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg cagcaacgcc caatcgcgcg tcatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggggtgcgct tttacgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct gcgcctcgcc gtcaatcccg atgacgacaa
     6001 cgccctcttg cgcgtcatca acttcccacc gcgcggcatc ggcgcgcgca ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat taccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgt cggcatcctc aaagacagtg gcctgaccga
     6241 acactaccga acccaaaaaa gcgacaacca agaccgcctc gacaacctag acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ctgaaaacat
     6361 ttcagacgac cccgccttcc ccatcctcgc tttcttgagc aacgccgccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgcag gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagtga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctgatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacattac gatggcgcaa caacgaatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagaaatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcgtc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggct tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagacaaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ctggaagaga tgtagaaggg agatggcaga gaccgtctga aaacgggaac
     7021 aacaattttt gcccaaacct ccgccgtcat tcccgcgcag gcgggaatcc ggacctttcg
     7081 gcacggaaac ttatcggata aaaggtttct ttagattcca cgtcctagat tcccgccgga
     7141 acataaatga cggacggtaa aagccgggta tgaataccca ccctctgtta tcactgagat
     7201 caataaggaa gaacattatg tcccaagttt ttaaagattt tgacttgtcc tccgtatgga
     7261 aaactaatag ttgggcagat gaaaactaca aagaagcccc gtttacccct gaaattttgg
     7321 ctgccgtaga aagtgagcta ggctataaat tgccgcaaag ttttattgca ttgatagcag
     7381 tacaaaacgg cgggatgttt gtcaaaaact gctttccgac cacacagaga aattcgtggg
     7441 cgaaagatca tgtgcaaatt tgcgaggtat cgggaatcgg ttttgaaaaa gaaggaagcc
     7501 tgtgcggcga aacagggcaa aaactttggc tggaagaatg ggagtatccg cccatcggcg
     7561 tgtattttgc caacgacccg tcaggcggtc atgccctgtt tgccttggac tatcgggagt
     7621 gcggcaaaaa cggagagccg aaagtggtgt ttgtcgaact cgcccccgat tttgaaacct
     7681 ttatccgcag cttgcggcat gaagatgagt ttattgacga agaaatataa aacggcggtt
     7741 gaaaaactga aatcattaag tgaaaacggg cgaaataacg ggtaaccgtt tgaatccgta
     7801 agggaaacag ttcggcggaa cgtgccatcc aagacctttg caaagaaaaa ctgtccccga
     7861 cagcattgac attattaaca gaacttatca aatttggagc tatcccaaat ataattcggt
     7921 tatcctgttg tatccattag attatatgct tcaattaaat gttgttctag ctcttgcact
     7981 aattgaggat tgcgaattcc tgatacaatc tcaaattctt ctgcatctat gcaaacacct
     8041 gcataaattt caataacaag ggaacgcaat aattgaagct cttctcttgt taaagaaata
     8101 ataatgtcat cacctgtgta attgattata ttcataataa ttttattttt gcttgtcaaa
     8161 gtaagtttta cctaaggttg gtctaaatgc agtcccacca tcttttgaat ttgggtctct
     8221 aattacaatt gctccagact tatcatccca aattgctctt atgtgtttgg attgtaatct
     8281 tcgaattccc aagaaaaaaa tcgtaataag tttgaaagtg tcaaatccca agtttctttt
     8341 gagcaatatt ctaatatttt atcaatttca cttttaataa tcttatgatc aaactgttct
     8401 aatattaatg cattagacca aaaaaaacct tctttattac aatgatggga aatccattta
     8461 ggagaacaaa tgcaaagtga aaaaatagat gagccttgtt ctccttcgat tccgatatcc
     8521 aaatctatcc atctatggaa attatctgga atttcggggg taaatttttc aaaatcaata
     8581 tcatataaat ttatgctttt taaatccaat ttaatcatta gggctgtcct agataa
//
LOCUS       DAXIEB010000085         8397 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000085 DAXIEB010000000
VERSION     DAXIEB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.085"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(174..1430)
                     /locus_tag="ACFOA3_001564"
     CDS             complement(174..1430)
                     /locus_tag="ACFOA3_001564"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6898550.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1465..2319)
                     /locus_tag="ACFOA3_001565"
     CDS             complement(1465..2319)
                     /locus_tag="ACFOA3_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6898551.1"
                     /translation="MNYALDALWWKLTDPTVRDLASLLTAPPLWQSGCELGVRELLGK
                     RGFRYLLALDADPAPLTEHLARHAPFGHRLGIYAEELSAFWFANAPHAELLAHNLTVS
                     GSDGSTQGAADFVARLNGKPCHIELTCKYYGGTGKPEDMRGLDPKDTLLGKAAKLTAQ
                     LGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFNAFEPPLNPYGWRGIYIQDW
                     AEYGFERREARYHLLDRMAYLAPARVAETETLNETEIRRIDNGLIAVLECRPDGFWHE
                     IERIMKAV"
     gene            complement(2390..3208)
                     /locus_tag="ACFOA3_001566"
     CDS             complement(2390..3208)
                     /locus_tag="ACFOA3_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6898552.1"
                     /translation="MTAPKPELPDYLGNIRIVLTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFNPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTLQQRLKDGQS"
     gene            3474..4259
                     /locus_tag="ACFOA3_001567"
     CDS             3474..4259
                     /locus_tag="ACFOA3_001567"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6898553.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            4451..5421
                     /locus_tag="ACFOA3_001568"
                     /pseudo
     CDS             4451..5421
                     /locus_tag="ACFOA3_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5426..6628
                     /locus_tag="ACFOA3_001569"
                     /pseudo
     CDS             5426..6628
                     /locus_tag="ACFOA3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            6752..8302
                     /locus_tag="ACFOA3_001570"
     CDS             6752..8302
                     /locus_tag="ACFOA3_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6898554.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEA
                     GMVSEPQQ"
BASE COUNT         2047 a         2086 c         2319 g         1945 t
ORIGIN      
        1 tagtctgtta acgaaatttg acgcataaaa atgcgccaaa aaattttcaa ttgcctaaaa
       61 ccttcctaat attgagcaaa aagtaggaaa aatcagaaaa gctttgcatt ttgaaaatga
      121 gattgagcat aaaattttag taacctatgt tattgcaaag gtctcaaacc ggtttattta
      181 cgttgcaata cggcggcgaa gaagccgtcg gtttggtgtc gggcggagtt gaggcgcagg
      241 tatttgccgg tatccaaatc gattttcaaa ccggcaagca gttcggcgca gttgacgggt
      301 tcaaattcgg gatgttcgga caggaaacgt tcgacttgca gctcgttttc ttcgggcagg
      361 atgctgcaag tggcgtacac caagcggcct tgcggtttta ccagtttgga ggcggcatcg
      421 aggatgctgt gttgctgttc caaaaggttg gccacggttt cggcggattg gcggtatttg
      481 agatcgggat tacggcgtaa agtacccaaa ccggagcagg gcgcatccac taaaacgcgg
      541 tcggctttgc ctgccagtcg ggcgatacgg ctgtcgtgtt cgctgctgat gcgttcaggg
      601 tggatgttgg tcagtccggc gcgggtcata cgcggtttga ggttggcaag gcgtttttcg
      661 gcgatatcga aggcgtagat tctgcctttg ttcgccattt gcgcaccgac agccaaggtt
      721 ttaccgccgg caccggcaca gaaatcgaca atgatttcgc cgcgttttgc gcccaccaat
      781 aaggcaagca gctggctgcc ttcgtcttga acttccagcg taccgtccaa aaacagctcg
      841 tgtttgttaa gcgcgatttt gtttttcagg cggatgcccc aaggcgaata aggcgttgcc
      901 tctgcatcgg cactttcggc ttgcaacaat ggcagcactt tatcgcgttt gcctttcaag
      961 gtattgacgc gtatatcgag cggcgcagct tggttgatgc tgcggccgaa ggcgaggatt
     1021 tcttcttcgc gccaatgctg ctgcagctgt tctaccagcc attgcggcaa ttctgcggcg
     1081 gtattcaggc cgtctgaaaa ctcggtttta cgggctttca aattgccgag aaactctatt
     1141 tcttcttcat caagcaggtc tttgatttgg ctgatgttgg tgcttctgcc gagaaccagt
     1201 gcggcgagtg cggctttgcg cggctgcgcg tgcggacggc gcagggcggt actgattttt
     1261 tgatagtggc gcagcgcggc aaaggcggtt tcggcgattt cgtggcggtc ttggcggccg
     1321 agttttttgt gttcgcggaa ataggcggag aggacggcat cggcaggctg tttgaaagtc
     1381 agcatttcag ccaaaacttt ggcggtatgg tcgagttgtg cggcgttcat cattaatggg
     1441 ttctcttaaa gttagtaaat ggtttcagac ggccttcata atgcgttcga tttcgtgcca
     1501 aaaaccgtcc ggccgacatt ccaaaacggc aatcaaacca ttgtcgatgc ggcggatttc
     1561 ggtttcgttc aatgtttcgg tttcggcaac acgcgcaggc gcgagatagg ccatgcggtc
     1621 gagcaggtga tagcgcgctt ctcggcgttc aaacccgtat tccgcccaat cttgaatata
     1681 gatgccgcgc cagccgtatg ggttaagcgg cggctcaaag gcgttgaaac cttgcggaaa
     1741 aaatccgatg ccgcgcacga tggaaacggg ttttacgtca agcggcagac cgtgttgccg
     1801 caaggttcgg atgccgtctg aagtgtgcgg cagaccgagt tgggcggtca gtttggcggc
     1861 ttttcccaac agcgtgtctt tggggtcgag tccgcgcatg tcttcgggtt tgcccgtgcc
     1921 gccgtaatat ttgcaggtca gttcgatatg gcagggcttg ccgttaagtc ttgccacaaa
     1981 atccgccgcg ccttgcgtgc tgccgtccga accggaaacc gtgaggttgt gcgcaagcag
     2041 ttcggcgtgc ggcgcgttgg caaaccaaaa tgccgacagt tcttcggcgt aaatgccgag
     2101 gcggtgtccg aatggggcgt gtcgggcgag gtgttccgtc agcggcgcgg gatcggcatc
     2161 caatgccaaa agatagcgga aaccgcgttt ccccaatagt tcgcgcacgc ccaattcgca
     2221 accgctttgc cacaaaggcg gcgcagtcag cagcgaggct aaatcccgca cggtagggtc
     2281 ggtcagtttc caccacaggg catcgagggc gtagttcatg gttttcctgt ttctgtattc
     2341 tgatttttcc tgaaatgccg tctgaaaaac ggattcagac ggcatgcgct taggattgcc
     2401 cgtccttcag acgctgttgc agcgtattga aaaaaccgcg caggatgtcg atgtcttcgg
     2461 tttgcgtgtt ggcgcgtccg aacaggctct gcatacggcg catcaggcgc tcgccgttgc
     2521 ggcggttgaa aaagccgatg tcgttcatca cgctttccat gtgggcgacc atgcctttga
     2581 tttgctcgtg ggtcgcagcg tggtcttctt gttgaagatg ggtcatgggc gaatcggttt
     2641 gactgaagat ttcgtagcac acgacctgca cggcttgggc gaggttgagc gagaaatagt
     2701 cgggattgcc gttgatggtc atcagtcggt tgcaggcttg gacttcttcg atgctcaagc
     2761 cgaaagtctc gttgccgaaa accagtgcca ctttctcgcc tcggttggcg gcctgcagta
     2821 attcgggcac caaatcgcgc ggggtttgca gcggcgcggt aatttcgcgg cggcggctgg
     2881 tcagggcgca ggcgatggtg gtgtcggcaa gggcttcgtc caaagaggcg gcaatggtgg
     2941 cattttccaa aacatctgcc gcaccggaag cgaggatgaa gctttcttcc ggtaatttaa
     3001 acgattgcgg atgctccgga ttgaacacgg gcggattctc cgtcatcggc gtagccatca
     3061 gattgggggc gacgatggtc agtttgtgca ggcccattgt tttcatcgcg cgcgcggcag
     3121 agccgatatt ggcgggatgg ctggtgcggg tcaggacgat gcggatgttg ccgagatagt
     3181 cgggcaattc gggtttaggg gcagtcatgt tcggttttcg gtcggaatgg aatagggtat
     3241 aatgcgccgt ctttttcgtt cggacggtca aatgccgtct gaaatgttct ttaacaacgt
     3301 ctgaaatgtt ctttaacaac gtcggaaatt taacgttgcg cctgaagcag acacgcgtca
     3361 ggcggtattt tacccgattt ggcaggattt accccaacca aataaactcc gattttgccg
     3421 ctccgaagga ctgtacgtcc gaaccggcgg tatgccaccc ttaaggaaat ctgatgaatc
     3481 cgtttttgaa tacagccttt aaagccgccc gccgtgccgg tcagatgatg atacgcgccg
     3541 cgggcaatct cgatgccgtc aaaaccgaca gcaaagcctt caacgatttt gtttccgatg
     3601 ttgaccgcaa ttccgaaatc atcctggttg aggctttgaa agaagcctat ccgcaccaca
     3661 aaatcacttg tgaagaaagc ggctcccacg gcaaagccgc agccgagtac gaatggatta
     3721 tcgatccgct cgacggcacg accaatttcc ttcacggtca tccccaatac gccatctcta
     3781 tggcgctgct gcacaaaggc gtgttgcaag aagctttggt ttacgccccc gaacgcaacg
     3841 acgtatatat ggcttcgcgt ggcaaaggcg cgttgctcaa cgaccgccgc atccgcgttt
     3901 ccaaccgcat cgaactgaac cgctgcctga ttggtaccgg cttccctgtt gtcgatcaaa
     3961 gcatgatgga caagtatctg gcgattttga aagatttctt ggccaaaacc gccggcggcc
     4021 gtcgtgaagg tgcggcttct ttggatttgt gcgccgtagc aacaggacgt tttgacggct
     4081 ttttcgagtt taacctcaaa ccgtgggaca ttgccgccgg tgcattgatt gttcaggaag
     4141 cgggcggtat cgtaaccgat atgtcgggtg aagacggctg gctggaaagc ggcgatgttg
     4201 tcgctgccaa tcccaaagtg ctggcgcaaa tgttgaaaat catttccgca cacgtttaaa
     4261 tttcagcccg ctcccgggaa tgcgggaaaa cgggcgggta tgccgtctga agtatcgcgc
     4321 cgatatttca gacggcattt ttactggcag cgggggcagg aaggtggaaa cggctaaatg
     4381 cccgaaactg ccgaagatgg aaacccatat ctgattaaac ggagaaccct gtacgatttg
     4441 tttatagtgg attaacaaaa accagtacgg tgttgcctcg ccttagctca aagagaacga
     4501 ttctctaagg tgctgaagca ccaagtgaat gggcaaagcc aacgccttta ccgcgccgat
     4561 taccgtcgtc aactacgacg aacaagccct caacaacacc gaggcgcgta ctttggtgga
     4621 tgccgtagcg aaaaaagacg cttccgtttg gcagttcggc ggcgaaagca acacgctgac
     4681 cggattgtat ttcagaggtt atcagcttga tgcgcgccaa ttcagcgtca acggtttgac
     4741 aggtatgtac ggcacgcaag gcacgaccag cgtgcaagtc ggctccgcac aactgattca
     4801 aggcgcgtcc accgccgtaa acggcatgaa ccctgatggc gcggtatccg gttccgtcaa
     4861 tatcgaaacc aaaaaagccg ctgatgaagg caaccgcaaa atcggtttgg cccggttcgg
     4921 caacaaccgc gcccaaggta cgttcgactt gggccaacgt ttcggcgaaa acaaagcatt
     4981 cggcgtgcgc gccaacggca aactgcgcca cggcgacacc ccgcgccacg gttacagcga
     5041 agacaacaaa gaatttgcgg taaatgccga ctatcgcggc gaaacactgc gcgtagcgtt
     5101 cgattccatc tacgcaaaac gcaaaaccaa cggcggtcgc gcgcgtatgc aggacattca
     5161 aaacgccggc ggacgcttgt cctgactttg ggcggacgtt tccaagcggt tgaatacacc
     5221 aataagaaaa aatcacgcgg aacggcagcc ggagctagca aggtagaagt gaaggcaaac
     5281 ggaaatattg agcatcaatt ggcatcgttt acagccgact atcccaattc ttgggagaac
     5341 tctgccaact tggatgccaa tatcaaagtc aacagcctgg ctttgtccga caccttgggc
     5401 ttcgtggata acaaataccg ttggcgcgcc cgatggtaaa accaacctgc tgccttcttg
     5461 gaactggcaa aataccgccg gccaaaccaa tatgcttacg tttgaatggg atgcgtttga
     5521 caatgcccaa attaccggcg gtatcggcta caacaaggcg cgttattacg gcacgctgat
     5581 ttccccgacc gtttgcggta caagcggtgc gagcgaccaa accgctacct gcacaagtgc
     5641 caagcaatac cacaccggca cggcgagcct gaccgaccaa tatttccgca cattgagtat
     5701 gaacctgacc gcacgcggcg aatttgaaac cggtccggta acacataact ggagcaccgc
     5761 gttcgaccgt gttatccgcc agcggaaaac cattcagagc ggcgatgcca aacgtttcag
     5821 cccgatgctg atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat
     5881 ggaagacttg gagcctgccg atatcaaaac cgatgattcc ggcgaaacga caatggcgaa
     5941 accgcgtgtc agccgtcagt ttgaagtggg cgtgcgtaaa aactggggca atttcgtaac
     6001 cacgttaaac gcgttccaaa tcaaacgccc gggctactgg cgcggtcata caactaccaa
     6061 gacaacaaaa ggggtaacaa caacgctgac ctatggcaac aattccgatt tcgcccgcta
     6121 taaggcgcaa ggcggcgtag ccggcgacga gcaaggtatg gaacgcaatc gcggtatcga
     6181 gttcaatgcc tatgctaacc tgttaaacaa aaccctgcgt ccgactttgg gcctgatgta
     6241 tctgcaatcg accgtgaaag actacccaaa ttcacgcgat atgctggtta acggcgtaca
     6301 agtcgccaat ccgcgcgtaa ttgccaaggc aggtttggaa tgggatacac cgtttgccaa
     6361 aggcttgact ttaaacagca atgtttcgta tttcggcaag tcttaccaag acacgcaaaa
     6421 acaatacgcc ttcccatcct ataccttggt tgatgtaggc gcgcgctaca aaaccaagct
     6481 cggcaaaaat acgttgaccg tcagcagctc ggtggaaaat ctgttcaaca aaaactactg
     6541 gcaggtacag cgcggccaat acgaccgcag cttcgccgtc gtcggtctgc cgcgtactta
     6601 ctggctgaag gcggaattgg atttctgatt tctccgcatc tggaaaatgc cgtccgaaca
     6661 ggtttcagac ggcatttctt ttaaagagat atggcgggat acgcttaaat aatgctattc
     6721 tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt cggttgcggc
     6781 agcattgctc ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag ccgaagaaag
     6841 cttgacgcag cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg aatcgcacca
     6901 atatgagcgc ggctggttta cctctacgga aacgacggtc atccgcttga aaccggagtt
     6961 gctgaataat gcccggaaat acctgccgga caacctgaaa acagtgttgg aacagccggt
     7021 tacgctggta aaccatatca cgcacggccc tttcgccggc ggattcggca cgcaggcgta
     7081 cattgaaacc gagttcaaat acgcgcctga aacggaaaaa gttctggaac gcttttttgg
     7141 gaaacaagct ccggtttccc ttgccaatac cgtttatttt aacggcagcg gtaaaatgga
     7201 agtcagtgtt cccgccttcg attatgaaga gctgtcgggc atcaggctgc actgggaagg
     7261 attgacggga aaaacggttt atcaaaaagg tttcaaaagc taccgcaaca gctatgatgc
     7321 gcccttgttc aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa aagcgcattt
     7381 cgattcggaa acttcagacg gcatcaatcc gcttgctttg ggcagcagca atctgacctt
     7441 ggaaaaattc tccctagaat ggaaagaggg tgtcgattac aacgtcaagt taaacgaact
     7501 ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc aatcccaacg gcagcatcgc
     7561 accttccaaa atcgaagtcg gcaaactggc tttttcaacc aagaccgggg aatcaggctc
     7621 atttatcaac agtgaagggc agttccgttt tgacacgctg gtgtacggca atgaaaaata
     7681 cggcccgctg gacatccata tcgccgccga acatctcgat gcttctgcct taaccgtatt
     7741 gaaacgtaag tttgcacaaa tttccgccaa aaaaatgacc gaggaacaaa tccgcaatga
     7801 tttgattgcc gccgtcaaag gcgaggcttc cggactgttc accaacaatc ccgtattgga
     7861 cattaaaact tttcgtttca cacagccgtc gggaaaaatc gatgtgggtg gaaaaatcat
     7921 gtttaaagac atgaagaagg aagatttgaa ccaattgggt ttgatgctga agaaaaccga
     7981 agccgacatc agaatgagta ttccccaaaa aatgctggaa gacttggcgg tcagtcaggc
     8041 aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa gtcttgacga
     8101 catcaacgag accttgcgcc tgatggtgga cagtacggtt cagagtatgg caagggaaaa
     8161 atatctgact ttgaacggcg accagattga tactgccatt tctctgaaaa acaatcagtt
     8221 gaaactgaac ggtaaaacgc tgcaaaacga accggagccg gattttgatg aagccggtat
     8281 ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag gtggcatttt
     8341 gttgtattgg aaactgccgt tttcaaccat tccttcagat aagccgtgtc ggattcg
//
LOCUS       DAXIEB010000086         8233 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000086 DAXIEB010000000
VERSION     DAXIEB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.086"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(281..1009)
                     /gene="rmpM"
                     /locus_tag="ACFOA3_001571"
     CDS             complement(281..1009)
                     /gene="rmpM"
                     /locus_tag="ACFOA3_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6898555.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1373..2323
                     /locus_tag="ACFOA3_001572"
     CDS             1373..2323
                     /locus_tag="ACFOA3_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6898556.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2436..3170)
                     /gene="fnr"
                     /locus_tag="ACFOA3_001573"
     CDS             complement(2436..3170)
                     /gene="fnr"
                     /locus_tag="ACFOA3_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6898557.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3375..4796
                     /gene="hemN"
                     /locus_tag="ACFOA3_001574"
     CDS             3375..4796
                     /gene="hemN"
                     /locus_tag="ACFOA3_001574"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6898558.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5089..6663)
                     /locus_tag="ACFOA3_001575"
     CDS             complement(5089..6663)
                     /locus_tag="ACFOA3_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6898559.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6988..8088
                     /locus_tag="ACFOA3_001576"
     CDS             6988..8088
                     /locus_tag="ACFOA3_001576"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6898560.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WMAACWVNRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2088 a         2308 c         2004 g         1833 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ctgaatctga atctgaatct gaatctgaat
       61 ctgaatctga atctgaatct gaatctgaat ccgaatccga atccgaacct gcaccccgat
      121 tctgacgata gccgtttcag aaaaccaccc aaaaaaacaa accccgcaaa agcgggggtt
      181 tgtttcacat catcgatgcc gatcgacgtt tcacgaatat tgcaacagta cccggtacaa
      241 aatccgctaa cctcatgcat cggcaagata ttgcctagcc ttagtgttgg tgatgattgt
      301 gtgccggcac aacctgacgg gttacgatgc tgcggatttt cacatccaca cggcggtcag
      361 gttcgataca tgcaatcaga gcctcacgtt ttttggcttt agagactttc gcacccagtt
      421 tggcaacttc ggcttcacaa acttgagtca tttgcgcttg agattcgccc aagccgacag
      481 cagaaattct agaaacaggt acgccgttgc tgaccaggtt gtttgccact acgtatgcgc
      541 ggcgttcgga cagggcctga ttgtatttgt cagaacccat aaagtcggta tggccttcga
      601 cgcggacaga ttggacattg gttcgactca ggcgttgcgc caatactttc aggttgtctt
      661 gagcttcggc gcgcaatgaa tccttatcga aaccgaacag ggttttggca gacagggaaa
      721 tggtttcatc aacatattgc ggagcctgct ccacaacgac gacaggcgcg ggtgcgggtt
      781 cgggttctgg ctcgggttcg ggggcagcaa ccgcatcgcc gcattctacg cgaccttggc
      841 ttgctttatc aaagtaggcg tttttccagc attcgccata gttgttgcgt acgatttcgt
      901 tcgactggcc gcttacggtg taaccctgaa cggacgcctc gcccgcaaca gcagtgccgg
      961 aagcgagcaa tgcaacgaat aatgcgctta atttcagctg tttggtcatt ttattccctc
     1021 attaaatttg tacagcagat acagaagcaa ctgctgaatt gccaacatac aggccgctgt
     1081 agaaacatgg cggcaaatcg gatatttcag tctccactat aaagaagcag ggggaggact
     1141 gtcaatactc aacgcctgaa aacaaccgta aaacagcctg tttcctgata gtctatcatg
     1201 ccgaaaaatc aattccgtgt cattacttta gggggatttt ttccatatcg cacgattgcc
     1261 gttgcacaaa aacaacaaac acaacagcaa agccccatac cgtaccggca agaaaatatg
     1321 ataaattata aacaatgttc agccacccga cacagaccca caccgacccg ccatgaaatt
     1381 acaacaattg aaatacgcct tagaagttta ccggcacaac ctgaacgttt ccgaagcggc
     1441 cgaagccttg tttacttcgc aacccggcat ctccaaacaa atcaaattgc tggaagaaga
     1501 aatcggcatt cagattttta tccgcagcgg caagcgcgtg gtttcggtct cgcagccggg
     1561 caaggtggtt ttggatattg cagaacgtat tttgcgcgat gttcagaaca ttaaaaatat
     1621 cggcagcgag tttaccggac aggacagcgg ttcgctgacg gttgccacga cgcatactca
     1681 ggcacgctat gccctaccct tgattgttgc cgattttgtg aaacgctatc cgaaagtcaa
     1741 cctgaccatc aaacagggga gccctgccgc catcgcccaa atggttactt cgggagagtc
     1801 tgatttggca attgttacgg aacgtataga cgaccacccc gaactgggaa gactttcctg
     1861 ctacgactgg acacacgcgg ttatcgtacc gaacgaccac cccttgctcg aatgcagaaa
     1921 ccccctccgt attgaagatt tggcgaggtt tccgctgatt acttatgaat ttgcattcaa
     1981 tgcgggcagc agcatcgcgc gggcattttc caaagcccgt ttggaacagc cggatgtcgc
     2041 attggcggcg gcggatacgg acgtattgaa gacttatgtg cgcttgggtt tgggcgtggg
     2101 actgatggcg aaaatggcgt acaacccgga tacggacggc gatttgcagc ttgtggatgc
     2161 ggcacacctg ttcgagccgt cgccgacgtg gattgctttg cgcagcgata cttatttgcg
     2221 cggatatgcc tacgacttta tccaagcgtt tgcgccgcac ctgacacgcg agaaggtgga
     2281 taggattctg tacacgccca tcagcgagga tttttcgatt taggcggctg ccggttttca
     2341 gacggcactt tgcggcagat acaacaaaca ggacagatgt tttcgtctgc cctgtgttta
     2401 ttgagaatgc cgtctgaaat gttcgtacgg gttaatcaaa tggcgtgcga gcagccggac
     2461 accatttttt tcaacacctg cagattgagg attttgatgt gcttatgctc gacggaaatc
     2521 aatccttcct gatgaaattt agataatgtg cggctgacgg tttcaagttt cagcccgaga
     2581 taactgccga tttcttcgcg ggacattctt aagatgaagt cgttggcagc aaaacctcgg
     2641 gaataaaggc gttgggaaag gttcagcagg aaggcggcaa tccgctcttc ggcgcgcata
     2701 ttgcccaaca gcagcataac accttggtcg cgcacgattt cacggctcat catgcggaag
     2761 aagtgcgtac gcaggctggg gatgttttgc cccagttctt cgatgtgggt aaacggcagt
     2821 tcgcacactt cgctgtcttc caaggcgacc gcgtcgcaac tgtgcacatg ggaacagatg
     2881 ccgtccatgc cgatgagttc gcccgacata aagaaacccg ttacctgatc gcggccgtcc
     2941 tgactggcga cggttgtttt gaagaagccc gaacggatgg caaagagcga ggtaaaggct
     3001 tcgccgacac agaacaggta ttcgcccttt ttcaggcggc ggctttgacg gatgacggca
     3061 tcgagttggc tgagctcgtt gggcagcagc ccgacaggca ggcagagttc ccgcaaagaa
     3121 caggaagaac acagcgtttt catctgatgt gtagtattat gcgaagccat accgtacctt
     3181 tttgtgcgct ttgccccatc atgattatag tggattaaat ttaaaccagt acggcgttgc
     3241 ctcgccttgt ccttatttaa atttaatcca ctatatgtgc ttattgacac atatcaagac
     3301 aggtttatca tactgtggca ttctaccaaa ctatccaaaa tttgactaac atcataaaac
     3361 cgcacacaca caccatgaaa atcattcaga tacagaacaa tcacaatgta aacgatgacc
     3421 gccccgagtt tgaccgcgcg ctgattgcca gcctgcccgc cagcggcccg cgctacactt
     3481 cctaccctac cgccgaccgt ttccatgacg gtttccgcga aggcgaatat atcaaagctt
     3541 tacatttgcg cggtatgggc gcgttaaaca aaccgctttc cctttacatt cacattccgt
     3601 tctgcaacac catctgctac tactgcggct gcaacaaaat catcaccaaa gacaaaagcc
     3661 gcgccgatgc ctacatcgaa tatcttgaaa aagaaatgga actgctcgct ccacatctga
     3721 acggacggca ccagcttgcc caactgcact tcggcggcgg cacgccgacc tttttgagcg
     3781 acgaacagat cgaacgtgtc ttccgcatga tacgcaaaca tttcgagtta atccccaccg
     3841 gcgaatactc catcgaaatc gacccgcgca aagtcagccg cgacaccgtc ctcatgctcg
     3901 gcaggctcgg cttcaaccgc atgagcatcg gcattcagga tttcgacccc aaagtgcagg
     3961 cggcggtcaa ccgcatccaa agttacgaag aaaccaaaga agtcatcgat gcggcgcgcg
     4021 aagcggggtt caaatccgtc agcgtcgatt tgatttacgg cctgccgcac cagacttcgg
     4081 aaagcatcaa aaccaccatc gataccgttt tgtcgctcga tcccgaccgc ctcgcccttt
     4141 atcactacgc ccacctgccg cacgtgttca aaccgcaacg ccgcatcgat accgtcgccg
     4201 ttcccgacag cgaagagaag ctcgatatgc tgcaatactg cgtccaaacc ctgacagagc
     4261 gcggctacgt cttcatcggc atggatcatt tcgccaaacc tgacgacgaa ctctccatcg
     4321 ccctcaaaga aggcttcctc cagcgcaact tccaaggcta ttcgacctac gcggattgcg
     4381 atttggtcgc catcggcgtg tcgtccatcg gcaaaatcgg cagcacctat tcccaaaacg
     4441 aacgcgacat cgatgcctac tatgccgcca tcgacgaagg cagactgccc atcatgcgcg
     4501 gttatcagct caatcaggac gacatcctgc gccgcaacat cattcaggat ttgatgtgcc
     4561 gtttcgcgct cgactatcgg atttacgaaa gtatgttcgg catcccgttc gaccgctact
     4621 tcaaagacga actggcggat ttggaaaaac tcgccggctt gggattggtg cgcctgaaca
     4681 gccacgggct gaccgttacc ccaaaaggac gcttcctcat ccgcaacatc gccatggtct
     4741 tcgattacca cctgcgccat aaagaaacca aggcgaaata ttcgcaaacg gtgtgattgc
     4801 gtctaacgta caaatgccgt cggcaggata tcataacgtt ccgcaccttt gtgtccgacc
     4861 gttccgaaac caagatatag tggattaaca aaaaccagta cagcgttgcc tcgccttgcc
     4921 gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcactatac
     4981 caaacggcat atcccgacag aacagattgt gcataaggca caagcccgca cattccatca
     5041 acaaaatgcc gtctgaacac gggttcagac ggcatcagta ttttacaatc agaatactgc
     5101 ctgtaaaacc aagtagcaga caaccgacaa tacggcggcg gcaggcaggg taatgaccca
     5161 cgccaaaccg atgggcttca tcagtttcca gttggcattg cggttgacca gaccgatgcc
     5221 gagtaccgcg ccgaccaaga tatgcgtact ggacacgggc agccccatca gcgacgcgcc
     5281 catcacgacg gatgcggcgg acagttcggc ggtaaaaccc gaggcggggt gcatttcggc
     5341 caaactcgta ccgacggttt taatcacctc tttaccgaca aaccacaaac cgacaatcag
     5401 cgcgatgccg aaagtcagca tcgcaatcgg ggggacgaca ttttgcgcgg caacgctgtt
     5461 ggtacgcaaa acatccataa tcgcggcaaa cggaccgatg gcgttggcga tatcgttcgc
     5521 accgtggctg aatgcgaagc cgcaggcggt aaagacctgc atccatgaaa acatctgaaa
     5581 ggtcgatttg cccaagtctt tacgcttgag gcttttggca aaaacaaacg tccccatcca
     5641 caccgccgcg cctatcataa agatggtcag gaagctgttg acgttgctca tccccaaatg
     5701 caggtttttc aagcccttga aaatcagcat agcggaaatc atcatcgcgc cgaacgaagc
     5761 gataaaggga atccaagaat gcagcgcttt gtaggaatcg acattgtttt tacggttgtc
     5821 gaacgcataa agaccgcggt aatactccga ttgcagctct tgcggatcga attcgggttc
     5881 gtcgtaaatt tgcgcgtcgt gcgccatttt ggtggcgtac tcgacttttt cggcttcgga
     5941 caaaccctcg aaaaacaggc ggtgccgttc tttataggcc tttttttcct gcttgatgcc
     6001 cttgagcgtg ccttccgccc aagcgttgta atctaagacg tttttcttga cgcgcgaaaa
     6061 cagaaaatag gacaccgcgc cgcccaacac gggcgacaat acccaagaaa caccaatacc
     6121 gcccagcttg ccccaacgta tcaaatcgcc cgatgcggca tcgttcatta ccgccataca
     6181 taccgcgctg ccgacaatgc cgccgataat ggaatgggtg gtagataccg gaagcccttt
     6241 tttcgaggca aacaacagcc acaacgccgc cgccaaaagc gcggacatca taataaacac
     6301 aaactgtatg ggttcgaaat caacaccctt caaatcgacg atgcctttgc gtatggtatt
     6361 ggttacctca ccgcccgcga tgaccgcgcc gctgacctca aataccgccg caatcagcaa
     6421 agcctgcggg atggtcagcg tacccgcacc gacgctggta ccgaaagaat tggcaacatc
     6481 gttgccgccg atgttgaacg ccataaacac gccgaacatc gtggcaataa tgaataaggc
     6541 ggcgtaattg taatgggtat aacccaagcc ccaataaata aaatagccga ctgtacctac
     6601 cagcatggcg gcaaagacgg cgttgatggt tttaacattt gcgctcattt ggatttgagc
     6661 catggcaatc ttcctttaat gtggtggaaa tcatgagtgt tgccgacact gtactgctgc
     6721 ctccggtcga actgccgttt cgggatatgg cagacgcatc cttccttctg aaccgcatcc
     6781 gaataccccc aaacctgacc gaatgcaaaa ctgctgccgg aaacggtttg tatgacactt
     6841 ctactactgg atgttgcggg cgcattataa tatttttcca tccgtcttga aacatttatt
     6901 tacactttat ttacactgcg gcggcaaatc ggtatacgag cgtcaataca cgttaaaatg
     6961 gcgttttgca ccagtttggg agtgatgatg gaaacacagc tttacatcgg catcatgtcg
     7021 ggaaccagta tggatggggc ggatgccgtg ctggtacgga tggacggcgg caaatggctg
     7081 ggcgcggaag ggcacgcctt taccccctat cccgacaggc tgcgccgcca attgctggat
     7141 ttgcaggaca caggcacaga cgaactgcac cgcagcagga ttttgtcgca agaactcagc
     7201 cgcctatatg cgcaaaccgc cgccgaactg ctgtgcagtc aaaacctcgc gccgtccgac
     7261 attaccgccc tcggctgcca cgggcaaacc gtccgacacg cgccggaaca cggttacagc
     7321 atacagcttg ccgatttgcc gctgctggca gaacggacgc ggatttttac cgtcggcgac
     7381 ttccgcagcc gcgaccttgc ggccggcgga caaggtgcgc cgctcgtccc cgcctttcac
     7441 aaagccctgt tccgcgacga cagggaaaca cgcgcggtac tgaacatcgg cgggattgcc
     7501 aacatcagcg tactcccccc cgacgcaccc gccttcggct tcgacacagg accgggcaat
     7561 atgctgatgg acgcgtggat gcaggcacac tggcagcttc cttacgacaa aaacggtgca
     7621 aaggcggcac aaggcaacat attgccgcaa ctgctcgaca ggctgctcgc ccacccgtat
     7681 ttcgcacaac cccaccctaa aagcacgggg cgcgaactgt ttgccctaaa ttggctcgaa
     7741 acctaccttg acggcggcga aaaccgatac gacgtattgc ggacgctttc ccgattcacc
     7801 gcgcaaaccg ttttcgacgc cgtctcacac gcagcggcag atgcccgtca aatgtacatt
     7861 tgcggcggcg gcatccgcaa tcctgtttta atggcggatt tggcagaatg tttcggcaca
     7921 cgcgtttccc tgcacagcac cgccgaactg aacctcgatc cgcaatgggt agaagccgcc
     7981 gcgttcgcat ggatggcggc gtgttgggtc aaccgcattc ccggtagtcc gcacaaagca
     8041 accggcgcat ccaaaccgtg tattctgggc gcgggatatt attattgagc acaaatgccg
     8101 tctgaaagac cgttccgccg ttcagacggc atttgtgcct ctttcattta caaaccctta
     8161 aaatcccttt atactcaaaa ctcgttcaaa aaaaaacaaa ccctgctatg aaaatcctgc
     8221 tcctcctcat ccc
//
LOCUS       DAXIEB010000087         8206 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000087 DAXIEB010000000
VERSION     DAXIEB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.087"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            140..1081
                     /gene="miaA"
                     /locus_tag="ACFOA3_001577"
     CDS             140..1081
                     /gene="miaA"
                     /locus_tag="ACFOA3_001577"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6898561.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYHLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1085..1804
                     /gene="tadA"
                     /locus_tag="ACFOA3_001578"
     CDS             1085..1804
                     /gene="tadA"
                     /locus_tag="ACFOA3_001578"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6898562.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPQQGEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIAAAHNTCIADCNVSRHAEINALAQASSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1809..2297
                     /locus_tag="ACFOA3_001579"
     CDS             1809..2297
                     /locus_tag="ACFOA3_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6898563.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2363..3115
                     /gene="rlmB"
                     /locus_tag="ACFOA3_001580"
     CDS             2363..3115
                     /gene="rlmB"
                     /locus_tag="ACFOA3_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6898564.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3177..4568)
                     /locus_tag="ACFOA3_001581"
     CDS             complement(3177..4568)
                     /locus_tag="ACFOA3_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6898565.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATVMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4583..4837)
                     /locus_tag="ACFOA3_001582"
                     /pseudo
     CDS             complement(4583..4837)
                     /locus_tag="ACFOA3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4875..5750
                     /gene="dapA"
                     /locus_tag="ACFOA3_001583"
     CDS             4875..5750
                     /gene="dapA"
                     /locus_tag="ACFOA3_001583"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6898566.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5759..6955
                     /gene="bamC"
                     /locus_tag="ACFOA3_001584"
     CDS             5759..6955
                     /gene="bamC"
                     /locus_tag="ACFOA3_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6898567.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(7012..7944)
                     /gene="pip"
                     /locus_tag="ACFOA3_001585"
     CDS             complement(7012..7944)
                     /gene="pip"
                     /locus_tag="ACFOA3_001585"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6898568.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT         2101 a         2699 c         1922 g         1484 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactatata aaggcatacg gtaaacacgc ccccttgccg
       61 tcggcgtgct tccgcaaacc aaaatataac gtcggcacgc tttccgctat aatccgtatt
      121 tttccaaacc tgaaacgtta tgccgacccc gaaagccttt gccctgctcg ggccgaccgc
      181 cggaggcaaa accgcgctcg ccctcaaaat tgccgaaacc ctgcctgtcg aaatcatcag
      241 cctcgattcc gcgctggttt accgcgatat ggacatcggc acggcgaaac cgtccgcctc
      301 cgaacgcgcc tttgtcccgc accaccttat cgacatcatc ccgcctaccg aatcatacag
      361 cgccgcccgc tttgtcgaag actgcacgcg cttggtcggg gaaatttcct cacgcggcag
      421 gtttgccctt attgtcggcg gcacgatgat gtatttccgc gccctgaccc aaggtttgaa
      481 cgatttgccc gaagccgacg cctgcctgcg tgccgacttg gacgaacaaa aacagatgta
      541 cggcttggat ttcctctacc gcaccctgca aaaagtcgac cctgaaaccg catgccgtct
      601 gaaaccgaac gacagccagc gtatcgaacg cgctttggaa gtttatcatc tgaccggcaa
      661 accgatgagc gaacacctcg gcagacaaag cccccatacg ctccccttcg atttgcatac
      721 cgccgccctg attcccgaaa accgcgcccg cctgcatgaa aatatcgccc tgcgtttcca
      781 cctgatgctt gaacagggct ttatcggcga agtagaaaac ctgcgccgcc tctatcccag
      841 cctgaccgcc gactcccctg ccatacgctg cgtcggctac cgtcaggcgt gggaacacct
      901 cgacggcgca accgacagac aaaccttcat cgaaaaaggc attgccgcca cgcgccaact
      961 tgccaaacgc caactgactt ggttgcgcaa aacaccttta gactgcgttg ccgacccgtt
     1021 ttcagacggc acttcatgca cacgcctgat tgaggcggca aaacggtttt tcggcgcata
     1081 aaccatgctg accacaccgc cgctcgcccc caaaaccgtc gccgccctgc acaggctcgg
     1141 tatccgtacg ctcgaagaac tgcggcaaaa cggttcggtc aaagcctttc tgctgctcaa
     1201 agcatcgggt ttgaccctca cgaaaagcac gctgtggcaa ctcgagtcgc tgctggacgg
     1261 cacgccgccg caagaaatgt cccaagcgca taaagctcgc ctgcttgccg aattgaaaaa
     1321 ccatccgccc gtcgccgcct ttccgcagca aggcgaaatg gaacacttca tgcgcgaagc
     1381 actgcgccaa gcagaacaat ccgccgccga cggcgaaata cccgtcgggg cagtcatcgt
     1441 ttcagacggc aaaatcatcg cggcggcaca caacacctgc attgccgact gcaatgtcag
     1501 ccgacacgcc gaaatcaacg ccttggcaca ggcaagcagc gaaatgcaaa actaccgcct
     1561 tgacggatgc gatatctaca tcaccctcga accctgcgcc atgtgcgcgt ccgcactgat
     1621 acaggcacga atcaggcgcg tgatatacgg tgcggcagaa cccaaaaccg gcgcggcagg
     1681 cagcatcgtc aacctgtttg ccgacaaacg cctcaatacg cacaccgcca tacggggcgg
     1741 aatcctgcaa gaagaatgcc gcgccgtatt aagccgtttt tttcagaaca aaagaaaagg
     1801 ttgaatccat gaccgcccta ctcgtcatcc tcgccctcgc cctgatagcc gtcggtacgg
     1861 cgggcatcgt ttaccccgcc ctgcccggat tggcattgat gtttgccgga acatggctgc
     1921 ttgcctactc cggcggctac caaatctacg gcgcgggcgt tttgtggacg gtcggactca
     1981 tcagccttgc cggcatactg gcggactatg tggcaggcat atgggggaca aaatataccg
     2041 gagcgggcaa gctcgccgtt cgcggcgcat tggccggcag catcatcggt atgtttttct
     2101 cccttcccgg actaatactc ggccccttta tcggcgcggc ggcaggcgaa ctgatcgatc
     2161 ggcgcaatat gcttcaggca ggtaaagcgg gcttgggtac gctgttgggg cttgtcgtcg
     2221 gtacggcatt caaaatcggc tgcgccgtat ccatcttgtt tatcctgttg gtgaaataca
     2281 tcgcatacct gttttaaccc cttcagacgg catcaggata aattgctata atgccgtctg
     2341 aaatttccga caaagcacca tcatggcaaa ccaaagacct atctacggct tccacgccgt
     2401 caacgcccga ttgtggcaaa accccaaatc catcgtcgaa ctctacatcc aagaaggcaa
     2461 atcagacgca cgcacgcgcg aagtgttgga aaaggcggca aacgaaaaca tccgcgtata
     2521 ttttgccgat gccgaccgcc tcaacgccat cagcaaaggc gcgcgccatc agggtgtggt
     2581 cggatttatc gatgcctcca aaaaccacgt ccacctcgaa gacgtattgg aaaacctcag
     2641 cgaaccgccg ctgttgctga tactcgacgg catcaccgat ccgcacaacc tcggcgcgtg
     2701 cctgcgtacc gccgacgcaa tgggcgtaca cgccgtcatc gcaccgaaag acaaaagcgc
     2761 ggggctgaac gccaccgtca gcaaagtcgc cagcggcgcg gcggaaaccg tcccctacat
     2821 caccgtaacc aacctcgccc gcaccctgcg cgagctgaaa gaatacggca tttggatcat
     2881 cggcaccgat atgggcggca acgccgacct ttaccattgc gatctgcccg acagcgcggc
     2941 atgggtgatg ggcaacgaag gcgacggtat gcgccgcctg acacgcgaac attgcgacat
     3001 gctggtgtcc atacccatgt tcggcacggt cgaaagcatg aacgtctccg tgtctgccgg
     3061 aatggtatta agcgaaaccc gccggcagcg cgtattaaaa aacgaaaaag cgtaagcctc
     3121 aaaaacaatg ccgtctgaaa cagggattct gtttcagacg gcattcgttt tcaaaatcag
     3181 tgttccaagt ggtcggtatc aaacttgacc gccgcctcag ttttatcttc gggcaagacc
     3241 aaattcagca agactgccgt aatgccgccg gcggaaatag agttttggaa caagacgggc
     3301 aggtttttaa acacttccgg ctcaaacgcg acacccaagc ccaaaccgac cgacgttgct
     3361 gcaattaccg cttcgcgcct gcggatgccg tggctgacca aaatccgcac gcccgcaatc
     3421 gcaatcaagc cgaacatcaa aaccatcgcg ccgcccaaca ccggactcgg aatcgtcgta
     3481 aacgcgcgac cgacaacggg gaacagaccc aacagcacca aaatcacggc aatatatttg
     3541 cccacatgac gcgaagccac gccggtcatc tgaatcacgc cgttgttttg cgcaaacgtc
     3601 gtcagcggca gcgaacccaa agccgtcgca atcaccgaca ccaagccgtc agccaacacg
     3661 ccgccgcgca aacgtttggt gtattcctcg ccttcaatcg gctggtcgga caccattacc
     3721 gttgcggtca aatcgccgac cgcctcaaac acgctcaaca ggaaaatcgc acccgccaca
     3781 ataaatgcgt gccagtcaaa agcaaaacca tatttaaacg gtacgggcag cgtaaccagc
     3841 ggcaggtttt gcagtgccga aaaatccacc ttgcccaaaa acagcgcgac gatatagccg
     3901 gcaatcagac cgaccgcaat gccgctcatc cgcagcagcg ggtttttcat gcagttgaac
     3961 accagcacaa tcagcagcac cagcgatgcc agccccaagt tttccatcga gccgaacgtg
     4021 ccgtccgcct ttgcgccgaa gccgccgccg aaatcggtaa taccgacgtg taccaaactc
     4081 aagccgatca gcatcaccac cacaccgctg accgtcggcg taatcacttt tttcaaatac
     4141 ggcagaagcc acgccgagaa acacaccaaa aacgcgccga caaacgatac gcccaagagc
     4201 gtcgaaatca tcgcatcctt agtcaaaccg ccctctttca tccccgcgcc cagcgcaatc
     4261 atgacggtaa cgaacgagaa attcaccgac tggatggaca gcatccccga accgaccggc
     4321 ccgaagcggt tgacctgcaa ataagtgccg acacccgacg caaccatcgc catcgacacg
     4381 agatacgccg tcatctccac cggcagttcc agcgcgccgc ccacaatcag cgcgggcgta
     4441 atcatcggca cgaaaatcgc caaaagatgg gtaaccgcgc tcaagagcgc attaccgaac
     4501 ggcggcctat cctccaaacc gtacaccaaa tcaggcgcat ccgccccttt tcccatatga
     4561 ccgctcataa tgtttccttg cttcaaatgt tgaaaaaacc aaagcgcgga ttgtagcgaa
     4621 tccgccatac cgcttcaatt tcgatttcgg acggcatacg cgcccgtccg gcaaacagat
     4681 tccgaaccgt cgccgccgga atatcgttta caaaacgcaa taacctgatt tcaaacacaa
     4741 aaatacaaac ccgacgcatc cgccgtcaag ccgccccgct catcccggac ccgccggatt
     4801 tcacacctgc ggtaaagtag gctacaatcg gaagcattac cgccccctac accacaactg
     4861 ataaaaggtt ctgtatgtta caaggtagcc tggttgccct gattaccccg atgaatcaag
     4921 acggcagcat ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca
     4981 cggacggcat cgtcgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac
     5041 acaccgccgt catcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg
     5101 gcacaggggc aaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg
     5161 gggcggacta caccctctcc gtcgtcccct attacaacaa accttcgcaa gaaggcatgt
     5221 accgccattt caaagccgtt gccgaagccg ccgcgattcc gatgatactc tacaacgttc
     5281 ccggcagaac cgtcgtcagc atgaacaacg aaaccatcct gcgccttgcc gaaatcccca
     5341 acatcgtcgg cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc
     5401 gcgcgcccga aggcttcgtc gtcctgtccg gcgacgacca caccgcccta ccgttcatgc
     5461 tctgcggcgg acacggcgtg attaccgtag ccgccaacgc cgcgcccaag ctctttgccg
     5521 atatgtgccg cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccggc
     5581 tgattccgat ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg
     5641 tatccgccct gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaag
     5701 gcggacaggc aaaagtccgc gccgccctga aagcctcagg acaactttaa atatagccat
     5761 gccgtccgaa ccgttcggac ggcataacgc aacaaacact ttaatatcca tcacacagga
     5821 tgacacgatg acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc
     5881 ctgctccggc agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct
     5941 gatcaaactc gaagtcccac ctgatttgaa caaccccgac caaggcaacc tctaccgcct
     6001 gcctgccggt tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt
     6061 ccaacagcct gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga
     6121 cggcagccaa cgctggctcg ttgtcgacgg caagtctcat gccgaaatct ggccgctcct
     6181 gaaagccttt tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca
     6241 aatggaaacc gagtgggcgg aaaaccgtgc caaaatcccc caagacagct tgcgccgcct
     6301 attcgacaca gtcggtttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt
     6361 ccgtatcgaa cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa
     6421 agaagtgtac ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc
     6481 caacctcgaa gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca
     6541 ggcggaaaac gcatcggcaa aaaaacctac ccttcccgcc gccaacgaaa tggcgcgtat
     6601 cgaaggcaaa agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgcgct
     6661 cgccctcgac cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgctttcct
     6721 ggttcaaaaa gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa
     6781 acgcctgctg ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta
     6841 tgccgagcct gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata
     6901 tgccggcaaa gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccgt
     6961 tcaaaacaaa agcagcttcc aaacagaagc tgcttttttg tgcggaactt tttacaacaa
     7021 ccgaggaaaa atatcctcaa ccgcctgaac caacgcatcc gccaaaggcg gatcgaacgc
     7081 acgatgccct gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca
     7141 cgcactctgc atcggcgtac acaaatcata ccgcccctgc acgataatgg tcgggatatg
     7201 ctgtattttg ccgatattgt tcaaaatcgc cctatcgccc tgcaaccagc cgccgttgac
     7261 aaaataatgg ttttccaaac gcgcgattgc cagcgaggca taagcatctt catccacttc
     7321 ctccggctcg aaacggatca gatagctttc ccaatccgcc caagcctttg cggcagacag
     7381 gcacacttct tcatcttgat gaaacagcaa cccgtgatac gcctcaatca gccggttccg
     7441 ccgattttca gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac
     7501 accgcccgcc tcgttcagcc acaccgtttc agacggtctg cacaaaaata tcccgcgcaa
     7561 caccaatccc tttacccgtt caggatgggt ttgggcataa gccagcgaca aagtgctgcc
     7621 ccacgaaccg ccgaacacca gccatttccc gatacccagc atttcacgga ctttttcaat
     7681 atccgccacc aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gcgaacgtcc
     7741 gcaaccgcgc tggtcgatga tgacgatgcg gaacacatcg ggattgaaaa aaccccgaca
     7801 ttcaggcgaa gcccccgcgc ccggcccgcc atgtaaaaaa atgaccggca caccgtcggg
     7861 attacccgac tcttcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg
     7921 aaaaggctgt tttatttcat acatcatctt ctcccatatt gcttctgaca atccgaacat
     7981 cccgacacac gacatcccgc acgaaacaca agccgccccg aaaggcaaat gccgaaaaat
     8041 cgcgatatag tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacggata
     8101 gtacggaacc gattcactcg tgcttaagca ccttagagaa tcattctctt tgagctaagg
     8161 cgaggcaacg ccgtaccggt ttttgttaat ccgctatatt ccgcca
//
LOCUS       DAXIEB010000088         8133 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000088 DAXIEB010000000
VERSION     DAXIEB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.088"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..215)
                     /locus_tag="ACFOA3_001586"
     CDS             complement(<1..215)
                     /locus_tag="ACFOA3_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898569.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLVVQSAEDKYADLKRHIHDIYQK
                     QLKDNGLVQSMSRKGNCLDNAAMESFFG"
     gene            complement(412..525)
                     /locus_tag="ACFOA3_001587"
     CDS             complement(412..525)
                     /locus_tag="ACFOA3_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6898570.1"
                     /translation="MSQKAAADQLNLPDCSILLQWLRLYHLNGINGLKPKP"
     gene            1598..2035
                     /locus_tag="ACFOA3_001588"
     CDS             1598..2035
                     /locus_tag="ACFOA3_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HFC6898571.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
     gene            complement(3571..3768)
                     /locus_tag="ACFOA3_001589"
     CDS             complement(3571..3768)
                     /locus_tag="ACFOA3_001589"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFC6898572.1"
                     /translation="MLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNM
                     VCEGFGRHYDTDENASSNIRK"
     gene            4353..5717
                     /locus_tag="ACFOA3_001590"
     CDS             4353..5717
                     /locus_tag="ACFOA3_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6898573.1"
                     /translation="MQHLTERMTQIAKLLNSSPNNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCSSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQY
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            6119..6583
                     /locus_tag="ACFOA3_001591"
     CDS             6119..6583
                     /locus_tag="ACFOA3_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898574.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWGNLQLWKRKNPIM"
     gene            6710..7999
                     /locus_tag="ACFOA3_001592"
     CDS             6710..7999
                     /locus_tag="ACFOA3_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFC6898575.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFAYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLCQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPVYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
BASE COUNT         2418 a         1670 c         1688 g         2357 t
ORIGIN      
        1 ccgaagaaac tttccattgc cgcattgtcc aagcagtttc ccttgcggga catactctga
       61 accagaccgt tgtctttcaa ctgcttttga taaatatcat ggatatgccg tttcaaatcg
      121 gcatatttgt cttctgccga ttggacaacc aattggtaat agaaggtgcc gcgtggcagt
      181 ccgacaatca ccaacagcag ttcaacggat ggcattgcct taaccctgcg acgagttgcg
      241 ttctttctac cgcacttctt tcccatagat taaggcatcg agctttttta gggcagccat
      301 ttccgcttta aggcaagcca attccgcaag cagttcttcc ttggttttca gatagtcggc
      361 tttttcgttt ccggcggatg ctgttttttc acgggctttc ttcctttggg tttagggttt
      421 gggctttaaa ccgttaatac cattcaaatg gtagaggcgc aaccattgca gcaagatgga
      481 gcagtcgggc aaattcagtt ggtctgcggc agctttttgg gacattccct accccgtcac
      541 caggcggatt gccccaagtt tgtattcgac cgaatatttt gtcgtatgct ttctgcgttt
      601 gatgccactc tctccgtgta atctgtattt tgtcacccat ctgcgtacca atgaatcgga
      661 aatagaaaga tggtctgctg ttccctgcca aatagtattg aacgacggca agtcggaatt
      721 catctgaata ttttgccata aaaaactgca ccccctaaag tcggtaaggt gtccaacttt
      781 tggggtgcag ttcagaagcg gtcttttttt gcctgccggt tttgaatcat cctccgtgta
      841 tattcccttg acgaaaaaaa tgatgatatt acggatacca aaactaaggt cgtatccgcc
      901 cccctactct ccctaagcaa agagatgaaa cagcgtatcg gctccctgcc ggttgaattt
      961 tccgaaaaaa cgcgacgtaa ccagcatcaa catatataag aacagcacaa atagcatcaa
     1021 tacatcaggc aacgaaaatg cagaataatg cacttaatgg tgtttggata tctgttgttt
     1081 tgtgctgtta gtaattcttc tttctgtgtt tacagtttag cagttgtaca gttttatagt
     1141 aatgtttaaa caatgactga tttattttaa atgcagatat tgtagaggat aaaaatggcc
     1201 aaagtccttt cagtaacatt tttgattttt tagcgagcct tctcatttcc ccggcgagat
     1261 cggcaatggc agcggtactt tggccgccga tatgcttaag ttcagtaacc ttacgccacc
     1321 aaaacccttg ctagctaagg gttaaacagc tcacttgaaa tctacttaag tctaatctaa
     1381 actatccaat atggatagat ttttaaacat agggcaagca gcaaaattat tgtagctgaa
     1441 agcacaatca ctcgctggtg gtctcaaaca cgtgccgact acctcgccga aaacactatc
     1501 agccgcgata aaccgtggga aaagctcgtt atcagccgcc gcacttggta ctatcgcggg
     1561 aaaccgatgc tgtctgaaac gcaacaggag aaaaataatg agccgttacc tgattacctt
     1621 tgatatggat accaactgcc tgaaagacaa ttaccacgga aataactata ccaatgccta
     1681 ctccgatatt aaaaccatct tggctagaca tggatttgag aacattcagg gcagtgttta
     1741 tctaggccgt gaaggcatca gtgaagcaca cggaacaata gccattcagg aactgaccgc
     1801 tcggtttgat tggttttact cctgtatttc aaacattaag ttttaccgcc ttgaaagtga
     1861 tttgaacgca caatttatcg ctgatggtgt gtatcaagcc aaacaggctt tccttcaacg
     1921 tgttgaacaa cttcgtatat ccctaacaga agctggattg tctgatgagc aaatcaatca
     1981 ggttctggaa aaacagaaat ttgaattgga aagtcctaac ctgaaattaa attaacctcc
     2041 tttactcacc aacatccgcc gcagctctgt cagtttttgg cgcgctgcgg cgatttctgt
     2101 gcgttttaga gcttcgggta gggtgtgaaa caactcactc gaaatttact taagtctaat
     2161 ctaaactatc caagcagtaa ttagtacaaa aaaggcaaac ttattttagg agcttaaaat
     2221 tgcagccgcg ataaaccgtg ggagagtctc ggcatttccc gcgccacttg gtacaaacgt
     2281 ggcaaaccga tgccgtctga aaccgtacaa caggagacaa aataatgctt gctcaaatcg
     2341 aactcacacc ctgccagcta atggtttacg taacttaagg ttactggatt tacgcactaa
     2401 ggttactgaa cttaagcata tcggcggcca aagtaccgct gccattgccg atctcgccgg
     2461 ggaaatgaga aggctcgcta aaaaatcaaa aatgttactg aaaggacttt ggccattttt
     2521 atcctctaca atatctgcat ttaaaataaa tcagtcattg tttaaacatt actgtaaaac
     2581 tgtacaactg ctaaactgta aacacagaaa gaagaattac taacagcaca aaacaacaga
     2641 tatccaaaca ccattaagtg cattattctg cattttcgtt gcctgatgta ttgatgctat
     2701 ttgtgctgtt cttatatatg ttgatgctgg ttacgtcgcg ttttttcgga aaattcaacc
     2761 ggcagggagt cgatacgctg tttcatctct ttgcttaggg agaatagggg ggggcggata
     2821 cgaccttagt tttggtatcc gtaatatcat catttttttc gtcaagggag tatatcgact
     2881 ctagaagaca ggtattagat actgcctttt cttacaagag tgatggtagg atggttctct
     2941 tcaagtcaat caaacaggaa agtatttctt ttctgtctga agatttgaaa aaggactgga
     3001 tgtttcacaa ggttaaaact gggaaaaaga tggatatggt tcagatgaaa tgctgagcgt
     3061 accccgtgtc tatttggaaa tgatgtcgcg gaaaacggga gtcccctact ccagtattct
     3121 ttaaattcta agcagaagac ttcttcgtcg gtcttttttt gttgtttggt ttgcatggag
     3181 taaaactgta caactgttga agcataaaac cagcaagaag gtaaaaaaga aagaagcagt
     3241 tctttggatt ttagatgtta ccgcaattag tttcctttcc taaaatttgt ttaaattatt
     3301 tgcaatatta atataaacga gatattaatg atgagaaatc aaaaaggcat aatgaatata
     3361 ttttgtacaa aatatttgca gtatttaaaa atgttgattt gtatatgaaa agttaaagat
     3421 gccaaaatgt acagttgcta aactgtaaaa ctgctaaagc aacaaaacat aaaaaggaat
     3481 gcagggatgc aatcactaca tctttttatt ccgtaagcat ttatgacttt acggtcaact
     3541 gctactctat gtttccagct tttcagctcc ctattttcga atattggacg aggcattttc
     3601 atcagtgtcg taatgccggc cgaaaccctc acaaaccata ttggttcttg tggcagcaac
     3661 acctatccgt ttgttcaagc gaccacaaga gtaacatgat tggctggtag cattggcttt
     3721 aatctcttcg atatgaactc catttttagc tgcaccttct ttcaacatat gcagcagtag
     3781 ggatgaggca actatcttct ggtggagttt gttgacttca atctgtatgc actgtaggtt
     3841 gagcagattc aattttttga tacagattat ccggctttgt tcggcaattc tgttggcgaa
     3901 cgtatagtag agctgtcgtc ttgcagcttt caatttgcgg taggtatttt accattcaat
     3961 gcgtagccgc tcggtacgtt tgagccaaat gttatcttcg ccatttgtac caattggttt
     4021 ttacattcgg ctgtgtttta gtaatttatt aatttcaatt atttgcaagg gaaaagacaa
     4081 ttgtttccgg ttaggaataa acctatccta ttgaatatat tgaagccaag tacgtctatc
     4141 aacactatat taaaacacag ccttttttaa tatagtagac acaatctttc cctatttatg
     4201 aaggtgatcg cttctttcag attcgtattt taatactttc tatttctaga aaatttgact
     4261 aaaataactc aattataaaa aattgcgcga ttttggtatt tatcatgaaa atttccagac
     4321 ctccggaatt taccctgttg caacaggaat atatgcagca tctcactgaa agaatgacgc
     4381 aaattgccaa gctgctgaat tcttccccaa acaatcctga tatagacatt cccgattttc
     4441 ttactgaaat caaagattat tcagaatttt ccgtgacaga tgaaaatgga acctacctgc
     4501 attgggacaa attccgccgg attcacacgg aagatacgcg gatgaaatgg cgcgccgtta
     4561 aggaaagccg caaaaaaatc caaaaaccaa ttgatttccc gtttgaacat cagttttggt
     4621 tctgcattcc cgactctttg caggcacggc ttcatttgat tgacaaaagc tgcagcagtt
     4681 ctatcggcac gtctagcttg ggtggtttcg gcagaagcga gcaaaacaga ttcttgctca
     4741 agtctctgat tatggaagaa gcgattacat ccgcccaact ggaaggtgcg gctaccacgc
     4801 gtaaagtggc caaggatatg ctcaaatcgc agcgtaaacc caaaacaaaa gacgaaatca
     4861 tgatagtgaa caactatcac ttgatgaaaa aagcggtaga attgaaaaat acgccgttaa
     4921 gtgttgaaat gattttggat ttgcaccgca ttgctaccag caacgctatt gaaaacaagg
     4981 ccgagcccgg acaattcagg cagtatgacg aaatctttat cgccgatatc aatggtaaca
     5041 gcctgtatca accaccgccg cacggacagg ttcatacgct gatggaagag gtgtgtgcgt
     5101 ttgccaataa tacctatgac ggcgtggaaa atccgtttat ccatccggtt gtccaagcta
     5161 ttatcctgca tttcctcatc ggctacatcc acccatttgg tgatggcaac gggcggacag
     5221 cgcgggcttt gttctattgg tttatgctca aaaacggcta ctggctattt gaatacatat
     5281 ccatcagccg tcttctgaaa aacgctcctg cccaatacgc caaatcctat ttgtatgcgg
     5341 aaactgacga tttagattta acctatttca tctattacca atgcgatatt atcaagcggg
     5401 cggttgctga tttggagcac tacatttctg acaaacaaaa acaccaacag gaattcaaag
     5461 cagcgattgc ccaatatact gaaaagatag gaaagttgaa ccaacggcaa attggtatcc
     5521 tgcaaaaagc agtggaagaa agcggaaaaa tctttactgc acaagaaatt gccaaccaat
     5581 acggcatctc cctgaatact gcccgtagcg atttgagcaa actgggagaa tatagattcc
     5641 tagtgccgtt caaatcagga aatgctttag agtatgttgc tcctcaggat ttattggaaa
     5701 ggttagaaaa aaaatagttt gctagcctaa aatgcagctt taaccgagtc aaaatcaata
     5761 cagtccgcac cttcaaaaag aagctgcgga ctgcttgctt tttgctctac aaatgatctt
     5821 tgtagctgat ttaaccaaga ttgtagcaat tttgctttcc gagcaagcag ggttagaaaa
     5881 ttcgatactt ttaattattg gctgtgtttt aatatgatgt tgataggcgt gtttggcttt
     5941 aaggtattca acaggatagg tttattccta accggaaaat aattgtcttt tcccttgcaa
     6001 gtaattgaaa tcaacagatt actaaaacac agccttacat tattggggtg actatcctgt
     6061 aaaatatgtc ctaaaacgtg gaaaccactt ttgctctgct aaattttaag gaatctttat
     6121 gttacatata cccccaacgg aacctgatcg atatgttagc agtattgtag ccttaaacgt
     6181 gcatagttct aacggtacag gcgattggca cagtgcagca gcccttaatg atgatgctta
     6241 cccccaagat ttctatattt atggcaaaga tcaaacacgt agcacgttac atttgcttgg
     6301 taacgttggt gtgattgatg gaacaaaccg cttcaatgag atgggctatt atcctgaaaa
     6361 catcccagtt tggctcgcag atcacccccg tgcttgcgtg gattatcttt acacagcagt
     6421 gttacaaact ggctcaatcg gtcgggtgat tttagatgat tggtttccaa gcgatgaaga
     6481 caagcaatca gtttatgact tacttaacca aatcgaaccg cacttgaata ctcaagaatg
     6541 ggggaattta cagctatgga aacgcaaaaa cccaataatg taatgctaac cgaacgagat
     6601 aaaaggcacg agaagcggta ttactgaatt tcatccgcaa tacgccatat acaattaaat
     6661 cagcagatgt gtctaaacgt cccgttgttc ccgtttgaaa gaatttttca tgatgaagtc
     6721 tatcctcacc gtatccggaa atcgtatgcg taaacccaga atcacctatt tggatgtttg
     6781 ggcaaacgat gaaagaatcg gtactttgga aaagggagcc atgtatcggt tcgcatacga
     6841 caatcccaat tcttcgttgc tgggcctgca ttatcaagac agaagcaagg tatatatcag
     6901 caacaacatg ccgcatatct ttgcacagta ttttccggaa ggctttttgg atgcacacat
     6961 cacaagcaaa tatgcttttc atgatgcgcc ttttgaagac aatgagatgc tgcgcttggc
     7021 aattctgggc agagagactt tgggtcggat acatgtgcgc tgtaatgacc cgctttttaa
     7081 tgaatggatc gacgggttgg agatgaaaaa tccaagaata ttgactgaac gggatttgct
     7141 gggcataaat gcccgacagg tttttcagca atatatggca gaaatcttcc atcacggccg
     7201 tttcgtcagt gtatccggga tacagcagaa gatgtcctta gatgccatca gcagaaatac
     7261 caagcaaact gcctcatata ttgccaaagg ttttgatgca tccgaatatc cttgcttggc
     7321 tgccaatgaa tttttatgca tgcaaaccat caaacaagcc ggcattgccg ttgcacagac
     7381 cagcctgtcg gaagattcat cagtcttatt ggtacgtcgg tttgatgtca gtgaacaggg
     7441 gtatttctta ggcatggaag actttaccag tctgtgtcag tattcagcag aagataagta
     7501 taaaggcagt tatgcggcta ttgcacagat tatccgacag atatccggca gaccagatga
     7561 agatttaatc catttcttta atcagcttgc tgccagttgc atattgaaaa acggcgatgc
     7621 acacctcaaa aatttttcag tactctatca tgacgaatac gatgttcgtc ttgcacctgt
     7681 ctatgatgta ttggatacat caatatacag ggttggaaca caaggaattt ttgatgctta
     7741 tgacgatacg ctggcattaa acctgactaa ccacggtaag aaaacatatc cttccaagaa
     7801 tacattgttg gattttgctg agaaatattg cgatttggga agagaagatg catcctttat
     7861 gatagataca atcgttcaag ctaaagaaca ggttcttgtt aaatactcgg atgtattgcg
     7921 tgagaatgaa tggttggcgc agaagtggca ttttatcccg gatgaaaatg aagaaggtct
     7981 accgtttaca ttccggtagc tgccgctgtc agagatggcc ggtcgacttt caccctgaaa
     8041 atcacttcat cttatggtgt ttgaaaccga gaaattagaa gaatcgtatt cggtaggaga
     8101 tatactggga agattggaaa actggtaaat cac
//
LOCUS       DAXIEB010000089         8091 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000089 DAXIEB010000000
VERSION     DAXIEB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.089"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            115..1284
                     /gene="prpF"
                     /locus_tag="ACFOA3_001593"
     CDS             115..1284
                     /gene="prpF"
                     /locus_tag="ACFOA3_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6898576.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1698..2897
                     /locus_tag="ACFOA3_001594"
     CDS             1698..2897
                     /locus_tag="ACFOA3_001594"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6898577.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAACILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSQAGLDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3005..3625
                     /locus_tag="ACFOA3_001595"
     CDS             3005..3625
                     /locus_tag="ACFOA3_001595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6898578.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3787..4230)
                     /locus_tag="ACFOA3_001596"
     CDS             complement(3787..4230)
                     /locus_tag="ACFOA3_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6898579.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4404..4772
                     /locus_tag="ACFOA3_001597"
     CDS             4404..4772
                     /locus_tag="ACFOA3_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6898580.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4817..5971
                     /locus_tag="ACFOA3_001598"
     CDS             4817..5971
                     /locus_tag="ACFOA3_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6898581.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6028..6900)
                     /locus_tag="ACFOA3_001599"
     CDS             complement(6028..6900)
                     /locus_tag="ACFOA3_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6898582.1"
                     /translation="MPILNHIALIGVGLIGGSFILDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6985..7797
                     /locus_tag="ACFOA3_001600"
     CDS             6985..7797
                     /locus_tag="ACFOA3_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6898583.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            complement(7826..>8091)
                     /locus_tag="ACFOA3_001601"
     CDS             complement(7826..>8091)
                     /locus_tag="ACFOA3_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6898584.1"
                     /translation="TETKIIFTTPTKGAKVGGTIPNKSMSKPPYHESNSISSLGLGVI
                     AGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT         1740 a         2205 c         2365 g         1781 t
ORIGIN      
        1 agattggggc gaaccccgac agtttgcgga atcaaacggc tttggtcgga gtggcagcct
       61 aatccactat aaaaatcgtg ggcagagccc acgctacata aggagaatct agaaatgccg
      121 caaattaaaa ttcccgccgt ttactaccgt ggcggtacat caaaaggcgt gtttttcaaa
      181 cgttccgacc tgcccgaggc ggcgcgggaa gcgggaagcg cacgcgacaa aatcctcttg
      241 cgcgtactcg gcagcccgga tccctacggc aagcagatag acggtttggg caacgccagc
      301 tcgtccacca gcaaggcggt gattttggac aagtccgaac gcgccgatca cgatgtcgat
      361 taccttttcg ggcaagtttc catcgacaaa ccttttgtcg attggagtgg caactgcggc
      421 aacctcaccg ccgccgtggg cgcatttgcc atcgagcaag gcttggtcga taaaggcaag
      481 attccttcag acggcatctg cacagtcaaa atctggcaga aaaacatcgg caaaaccatt
      541 attgcccatg taccgatgca aaacggcgca gttttggaaa caggcgattt tgagctcgac
      601 ggcgtaacgt tcccggcagc cgaagtacaa atcgaatttc ttgatccagc cgacggcgaa
      661 ggcagtatgt tcccaaccgg caatttggtc gatgaaattg atgtgccgaa tataggccgt
      721 ttgaaagcca cgctcatcaa cgcgggcatt ccgaccgttt tcctgaatgc cgccgacttg
      781 ggctacacgg gcaaagagtt gcaagacgac atcaacaacg atgccgcagc tttggaaaaa
      841 ttcgagaaaa tccgcgctta cggtgcgctg aaaatgggtc taatcagcga cgtatccgaa
      901 gctgccgccc gcgcgcacac gccgaaagtc gccttcgtcg cgcccgccgc cgattacacc
      961 gcctccagtg gcaaaaccgt gaatgccgcc gacatcgatt tgctggtacg cgccctgagc
     1021 atgggcaaat tgcaccacgc gatgatgggt accgcctctg ttgccattgc gaccgccgcc
     1081 gccgtgcccg gtacgctggt caaccttgcc gcaggcggcg gaacgcgtaa agaagtgcgc
     1141 ttcgggcatc cttccggcac attgcgcgtc ggtgcagccg ccgaatgtca ggacggacaa
     1201 tggacggcca ccaaagcggt tatgagccgc agcgcacgcg tgatgatgga aggttgggtc
     1261 agggtgccgg aagattgttt ttaaattgac gtagcatggg tttgcccgcg agccataaac
     1321 aggtcgtctg aaaaacaagt aaacatcaaa tcactgacca ttcctttccc ttgccctgtg
     1381 gcggaaggcg gcaaatcaca aggaagaaca cggaaacccc gataaaagac agcttcccgt
     1441 attaccgtca ttcccgcgca ggcgggaatc tagacctgtc aatatggagg attggtaggg
     1501 gaaaacaggt ttcgtgagtt ctacattctg gattcccgcc acagcctgtc ctcgcgtagg
     1561 cggggacgga ataacgatag aaaatgcggc atacgctttg cccaaagagg ccgtctgaaa
     1621 caccttgcgc ctgatgtctg cctttttcag acgaccccac accaaaaaaa caaccacaaa
     1681 ctacaaggag aaacatcatg tccgaccaac tcatcctcgt tctgaactgc ggcagttcat
     1741 cgctcaaagg cgccgttatc gaccgaaaaa gcggcagcgt cgtcctaagc tgcctcggcg
     1801 aacgcctgac cacgcccgaa gccgtcatta cgttcagcaa agacggcaac aaacgccaag
     1861 ttcccctgag cggccgaaat tgccacgccg gcgcggtggg tatgcttttg aacgaactgg
     1921 aaaaacacgg tctgcacgac cgcatcaaag ccatcggcca ccgcatcgcc cacggcggcg
     1981 aaaaatacag cgagtctgtt ttgatcgacc aggccgtaat ggacgaactc aatgcctgca
     2041 ttccgcttgc gccgctgcac aaccccgcca acatcagcgg catccttgcc gcacaggaac
     2101 atttccccgg tctgcccaat gtcggcgtga tggatacttc gttccaccaa accatgccgg
     2161 agcgtgccta cacttatgcc gtgccgcgcg agttgcgtaa aaaatacgct ttccgccgct
     2221 acggtttcca cggcaccagt atgcgttacg ttgcccctga agccgcatgc atcttgggca
     2281 aacctctgga agacatccgc atgattattg cccacttagg caacggcgca tccattaccg
     2341 ccatcaaaaa cggcaaatcc gtcgatacca gtatgggttt cacgccgatc gaaggtttgg
     2401 taatgggtac acgttgcggc gacatcgacc cgggtgtgta cagctatctg acttcccaag
     2461 ccggtttgga tgttgcacaa gttgatgaaa tgctgaacaa aaaatcaggt ttgctcggta
     2521 tttccgaact ttccaacgac tgccgcaccc tcgaaatcgc cgccgacgaa ggccacgaag
     2581 gcgcgcgcct cgccctcgaa gttatgacct accgcctcgc caaatacatc gcttcgatgg
     2641 ctgtggcctg cggcggcgtt gacgcactcg tgttcaccgg cggtatcggc gaaaactcgc
     2701 gtaatatccg tgccaaaacc gtttcctatc ttgatttctt gggtctgcac atcgacacca
     2761 aagccaatat ggaaaaacgc tacggcaatt cgggtattat cagcccgacc gattcttctc
     2821 cggctgtttt ggttgtcccg accaatgaag aactgatgat tgcctgcgac actgccgaac
     2881 ttgccggcat cttgtagcca aaaaagggac gagtccgcaa aaatgccgtc tgaaattttc
     2941 agacggcatt ttgtttggat tttgggcgta caatacggaa aacatgacga taaggacaca
     3001 aactatggca cagtttttcg ccgttcatcc cgacaacccc caagaacgcc tcatcaagca
     3061 ggcggtcgaa atcgtcaata aaggcggcgt ggtcgtttat ccgaccgatt cctgttatgc
     3121 cttgggttgc aaactcggcg ataaggcggc gatggaacgc atactctcca tccgaaaaat
     3181 cgatttgaaa caccacctga ccctgatgtg cgccgatttg agcgagttgg gcacatacgc
     3241 caaaatcgac aacgtacagt ttcgtcagct taaggccgcc acgcccgggc cttatacctt
     3301 tatcttgcag gcgacgaagg aagtgccggc gcgcacgctg cacccgaaac gcaaaaccat
     3361 cgggctgcgt attcccgata atgccattgc acaagccctg ctgggggaat tgggcgagcc
     3421 gcttttaagc tgcaccctga tgctgcccga agacggcgaa ccattgaccg atccttatga
     3481 aatccgcgag cgtttggaac acgccgtcga tttggtgatt gacggcggct ggtgcggaac
     3541 cgagccgacc accgtcgtcg atatgaccga cggcacggaa ttggtgcgcc aaggttgcgg
     3601 cgatacggcg gtgttcggtt tgtagggaaa ccgatgccgt ctgaagcatc ggctgttcag
     3661 acggcattgc gcgccttgcc ggcggcagtc cgaaatgccg gcacgtatcg cgctcggtcg
     3721 gaatatccgt ttgaaacggc aaacaaaatg ccgtccgaac gcgggttcgg acggcatttt
     3781 gatgcattac tgcaccgcaa tcggaattct cggttcgtag agcaggtcgt aggtcggctt
     3841 gttgagcagg tcttggagcg tgaaaccgtc cagatacgtg aaaaacgact tcattgcacc
     3901 gccgagtatg cctgtcagac ggcaggacgg tgtaatcagg cattcgttgt tcgaacccat
     3961 acattcgacc agctgcatcg gttcgaggtg gcggacgacc gagccgatgt tgatgcggtc
     4021 gggcggtgtg gcaagccgca gaccgccgcc ttttccgcgc acgctgtgga ggaagccgcc
     4081 tttgaccagc gcggtaacga ccttcatcag atggcttttg gaaatgccgt aggttacggc
     4141 gatggtactg atgttgacca gcgcatcgtc gttgatggca gtgtagataa ggacgcgcag
     4201 cccgtagtcc gtatgttgtg tcaaatacat gattttctcg gtatggattg ttattcttat
     4261 cggtacggtt taaggttcac ggacaatacc ttaatggttg aaaccctgtc cgtcggggcg
     4321 gtagaatgca gcctgtctgc ggcggtatgc cgtctgaaac atccgcgcta ccgtttgaga
     4381 atttgttatt gtaactcaaa atcatgaaac cgttgaaacg acatcccgcc cttatcgggc
     4441 tttcgcgtga ccaccaccat tcgctttccc tgtgcgtgcg tctgttgcgg acgccggaag
     4501 aaaggcatcg ggacgaactc gaaccgcatt tttccgaatt ggaaacccat tttcgcgaag
     4561 aagaaaccaa gtttgcccca atttggcaga atgtcgcccc cgaattgaaa caacgtttcg
     4621 agaaagacca cgcccgactg cggcagatga tggcaagccc cgaatacggt aacgcggcgt
     4681 ggaataccgc ttttgccaca accctgcgcg accacgcgcg ctttgaagaa cgcgagctgt
     4741 ttcccgccgc cgaaccgttt ttgccggcat gattccgttt tgcggcaaat atattaatga
     4801 taaacaagga acacacatga aatttaccaa gcaccccgtc tgggcaatgg cgttccgccc
     4861 gttttattcg ctggcggctc tgtacggcgc attgtccgta ttgctgtggg gtttcggcta
     4921 cacgggaacg cacgagctgt ccggtttcta ttggcacgcg catgagatga tttggggtta
     4981 tgccggactg gtcgtcgtcg ccttcctgct gaccgccgtc gccacttgga cggggcagcc
     5041 gcccacgcgg ggcggcgttc tggtcggctt gactatcttt tggctggctg cgcggattgc
     5101 cgcctttatc ccgggttggg gtgcgtcggc aagcggcata ctcggtacgc tgtttttctg
     5161 gtacggcgcg gtgtgcatgg ctttgcccgt tatccgttca cagaatcaac gcaactatgt
     5221 tgccgtgttc gcgctgttcg tcttgggcgg cacgcatgcg gcgttccacg tccagctgca
     5281 caacggcaac ctaggcggac tcttgagcgg attacagtcg ggcttggtga tggtgtcggg
     5341 ttttatcggt ctgattggta cgcggattat ttcgtttttt acgtccaaac ggttgaatgt
     5401 gccgcagatt cccagtccga aatgggtggc gcaggcttcg ctgtggctgc ccatgctgac
     5461 cgccatgctg atggcgcacg gcgtgatgcc ttggctgtcg gcggctttcg cgtttgcggc
     5521 aggcgtgatc tttaccgtgc aggtgtaccg ctggtggtat aaacccgtgt tgaaagagcc
     5581 gatgctgtgg attctgtttg ccggctatct gtttaccgga ttggggctga ttgcggtcgg
     5641 cgcgtcttat ttcaaacccg ctttcctcaa tctgggtgtg catctgatcg gggtcggcgg
     5701 tatcggcgtg ctgactttgg gcatgatggc gcgtaccgcg ctcggtcata cgggcaatcc
     5761 gatttatccg ccgcccaaag ccgttcccgt tgcgttttgg ctgatgatgg cggcaaccgc
     5821 cgtccgtatg gttgccgtat tttcttccgg cactgcctac acgcacagca tacgcacctc
     5881 ttcggttttg tttgcactcg cgcttttggt gtatgcgtgg aagtatattc cttggctgat
     5941 tcgtccgcgt tcggacggca ggcccggttg agacaaaccg ccgcagattt cgggtctggg
     6001 cggtttgctt ttcagacggc agggcggtca gttgccgtcc agccagcggt cgcgtgtggt
     6061 tttggcttct tcaaaatagc ggtacagggc ttcgcggtcg tcggtggtca ggatgtttgc
     6121 caaaacgtcc aactgtttgc ccaagccttg aaccagttgc agcaggctgt ctttgttggc
     6181 aaggcagatg tccgcccaca cggcgggatg accggaggcg atgcgggtga agtcccgaaa
     6241 gcccgtggcg gcgaatttca gatattcctg tccgtcggga tggtcgagaa tctggtggac
     6301 ataggcgaag gcggtcaggt ggggcatatg ggagacggcg gcgaaaaccg cgtcgtggcg
     6361 ttgcgcgtcc atcgtataaa tttccgcccc gaccgcgtgc cacaggtttt ctatcaaggc
     6421 aatgccgtct gaatgttcgc cgccgtgtgg cgtgatgatg agttttctgt ggcggaacag
     6481 cccgaactgc gcggcttgcg caccgcttct gtccgaaccg gcaatcgggt gggcggcgat
     6541 gcagcggtgc aggcggtcgg gcagacagcg gcggaaggct tcgatgaccg aagatttggt
     6601 gctgccgaca tcggaaatcc aagtgtgttc cggcaaaacg gggcgcagcg cggtcaaaat
     6661 ggcgggaacg gtggcgacgg gcgtggcaat cagtaccaag tctgcaccac cgatgctgtc
     6721 cgcgtcgatg gcaacggaag cccggtcaat cacgccgcgt tccaatgcac gttcgaggtt
     6781 gtcgcggtcg gtgtcgatac cggtaacggt gcggacgagt ccctgccttt tgaggtcgag
     6841 aatgaacgaa ccgccgatca gccctacacc gatgagggca atatggttca aaatgggcat
     6901 ttgtgtaaac ggttttcgca aagtaccgtc atggtagcct atcggcggaa tatgccgcaa
     6961 ggtcggcagg aaaaaggaga agaaatggac aaaatcagag ttgccgccgt gcagatggtg
     7021 tcgggcgtgt cgccggaaac caacgtcgcc gccatgaaac gcctggtcgc acgggcggcg
     7081 gagcagggtg cggattgggt gctgctgccc gaatattggg tgctgatggg cgcaaacgat
     7141 accgacaaac tcgcgcttgc cgaacctttg ggcggcggac gctttcagac ggcattgagt
     7201 gaaacggcga aagaatgcgg tgtggtgctg ttcggcggaa ccgtgccgct gcaaagcccc
     7261 gaggcgggca aggtcatgaa tactttattg gtgtacgggc gcgacggcaa acggacgggg
     7321 ctgtaccaca aaatgcacct cttcggcttt tccggtttgg gcgaacgcta tgccgaagcc
     7381 gataccatcc gcgcgggcgg ggatgtgccg cacttgtcgg cagaaggcgt gtcggtggcg
     7441 gcgggcattt gttacgatgt ccgctttccc gaatttttcc gccgccagct gccgtttgac
     7501 gtattgatgc tgcccgctgc gtttacgcac acgacgggca aggcgcactg ggagctgctt
     7561 ttgcgcgcgc gtgctgtcga aaaccaatgt tatgttgtgg cggcagcgca gggcgggctg
     7621 cacgaaaacg gcaggcgcac gttcggacac agtatgattg tcgatccgtg gggcgacgtg
     7681 ttggacgtat tgcccgaagg cgaaggcgtt gttacggcag acatcgatgc caaccgcctg
     7741 aacagcgtcc gcaaccgcct gcccgccttg aaataccggg ttttggatgc cgtctgaagg
     7801 ttcagacggc atcggtgtcg gggaatcaga agcggtagcg cacgcccaat gagacttcgt
     7861 gggttttgaa gcgggtgttt tccaagcgtc cccagttgtg gtagcggtat ccggtgtcta
     7921 aagtcagctt gggcgtgatg tcgaaaccga caccggcgat gacaccaaga cccaagctgc
     7981 tgatgctgtt gctttcgtga tagggaggtt tgctcatcga tttgtttggg atcgtgcctc
     8041 caactttagc gcctttcgtt ggtgtagtga aaataatctt ggtttctgtt t
//
LOCUS       DAXIEB010000090         8045 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000090 DAXIEB010000000
VERSION     DAXIEB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.090"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            596..1471
                     /gene="prpB"
                     /locus_tag="ACFOA3_001602"
     CDS             596..1471
                     /gene="prpB"
                     /locus_tag="ACFOA3_001602"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6898585.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIVRTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDTMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1556..2710
                     /gene="prpC"
                     /locus_tag="ACFOA3_001603"
     CDS             1556..2710
                     /gene="prpC"
                     /locus_tag="ACFOA3_001603"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6898586.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCIHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWAAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3036..3980
                     /gene="gntB"
                     /locus_tag="ACFOA3_001604"
     CDS             3036..3980
                     /gene="gntB"
                     /locus_tag="ACFOA3_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis L-arginine gamma (S)
                     hydroxylase"
                     /protein_id="HFC6898587.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGIHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            3967..4878
                     /locus_tag="ACFOA3_001605"
     CDS             3967..4878
                     /locus_tag="ACFOA3_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6898588.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            4969..7575
                     /gene="acnD"
                     /locus_tag="ACFOA3_001606"
     CDS             4969..7575
                     /gene="acnD"
                     /locus_tag="ACFOA3_001606"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6898589.1"
                     /translation="MAANQRYRKPLPGTDLEYYNARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDVQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANCFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVCQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
BASE COUNT         2136 a         1832 c         1962 g         2115 t
ORIGIN      
        1 cttgtcctga tttaaattca atccactata tatttaaatt taattaaata atataaaaat
       61 caaatagata acaaactaca tcggattgct taaaaataat acattgtttt tatgtataaa
      121 atattttata agttttcagg attttgatta tcaaaaattt ttcttgattt cctgacaatt
      181 ttattgaaac aaataattaa aaattaatct agtttaatca tggaattaaa ataaaatatt
      241 aaaattatgt aatgagtctc cttaaaaatg tttgacattt tcagtcttgt gttttagatt
      301 agcgaaaaat aaaactacac aatactacaa aggaacatta ctatgaaacc aattcagatg
      361 ttttcccctt ttctgaataa tccccttgtt ttcttcttgt ctgcggtttt gccgcataat
      421 tccgaacggt ctgctgtttt tctttgattc gttttaaata tcaataagat aatttttccc
      481 atatattttt aatgattgga ttgggatgcc cgacgcgtcg gatggctgtg ttttgccgtc
      541 cgaatgtgat ggaagcctgt ccatactgaa aaaaagtcta taaaggagaa atatgatgag
      601 tcaacactct gccggagcac gtttccgcca agccgtgaaa gaatcgaatc cgcttgccgt
      661 cgtcggttgc gtcaatgctt actttgcacg attggccacc caaagtggtt tcaaagcaat
      721 ctatctgtct ggcggcggcg tggcagcctg ctcttgcggt atccctgatt tgggcattac
      781 cacaatggaa gacgtgctga tcgacgcacg acgcattacg gacaacgtgg atacgccttt
      841 gctggtggac atcgatgtgg gttggggcgg tgcattcaat attgtccgta ccattcgcaa
      901 ctttgaacgc gccggtgttg cagcggttca catcgaagat caggtagcgc aaaaacgctg
      961 cggccaccgt ccaaacaaag ccatcgtttc taaagatgaa atggtcgacc gtatcaaagc
     1021 tgccgtagat gcgcgtgttg atgagaactt tgtgattatg gcgcgtaccg atgcgctggc
     1081 ggtggaaggt ttagatgccg ctatcgaacg cgcccaagct tgtgtcgaag cgggtgcaga
     1141 catgattttc cctgaagcca tgaccgattt gaaaatgtac cgccaatttg cagatgcagt
     1201 gaaagtgccc gtgttggcga acattaccga gtttggttct actccgcttt atacccaaag
     1261 cgaactggct gaaaacggcg tgtcgctggt gctgtatccg ctgtcatcgt tccgcgcagc
     1321 aagcaaagcc gctctgaatg tttatgaagc gattatgcgc gatggcactc aggccgcagt
     1381 ggttgacact atgcaaaccc gtgccgagtt gtacgagcat ctgaactatc atgccttcga
     1441 gcaaaaactg gataaattgt ttcaaaaata agtaaccgct ttcagacggc ctttcaacaa
     1501 atccgcatcg accgtctgaa aaataaaacc cataaaaata caaaggagaa ataccatgac
     1561 tgaaactact caaaccccga ctttcaaacc taaaaaatcc gttgcgcttt ctggcgttgc
     1621 ggctggtaat accgaattgt gtaccgttgg ccgtaccggc aacgatttga gctatcgcgg
     1681 ttacgacatc ttggatttgg cacaaaaatg cgagtttgaa gaagtcgccc acctgctgat
     1741 tcacggtcat ttgcccaata aattcgagct ggccgcttat aaagccaagc tcaaatccat
     1801 gcgcggcctg cctatccgtg tgatcaaagt tttggaaagc ctgcctgcac atacccatcc
     1861 gatggacgtg atgcgtaccg gcgtatccat gctgggctgc attcatcctg aacgtgaaag
     1921 ccatccggaa agcgaagcac gtgacatcgc cgacaaactg atcgccagcc tcggcagcat
     1981 cctcttgtac tggtatcaat attcgcacaa cggcaaacgc attgaggttg aaagcgacga
     2041 agagaccatc ggcggtcatt tcctgcacct gttacacggc aaacgcccaa gcgaatcaca
     2101 catcaaagcc atgcacgttt cactgattct gtatgcagaa cacgagttca acgcttctac
     2161 ctttaccgcc cgcgtgattg ccggtacagg ctctgatatg tactccagca ttaccggtgc
     2221 aatcggcgcg ttgaaaggtc cgaaacacgg tggcgcgaac gaagtggctt acgatattca
     2281 aaaacgctac cgcaatgccg acgaagccga agccgacatc cgcgaacgca tcggccgcaa
     2341 agaaatcgtg atcggtttcg gtcatccggt gtacaccatt tccgaccctc gcaacgttgt
     2401 cattaaagaa gtggcacgtg gtttgagcaa agagaccggc gatatgcgcc tctttgacat
     2461 tgccgaacgt ttggagagcg tgatgtggga agagaaaaaa atgttcccga atctggactg
     2521 gttctctgcc gtttcctatc aaaaattggg cgtaccgacc gctatgttca caccgctgtt
     2581 cgtaatttcc cgtacaaccg gctgggcggc acacgttctc gagcaacgca aagacggcaa
     2641 aatcatccgt ccgagcgcga actacacagg ccctgaagat ttggcgtttg tggagattga
     2701 agaacgctga gacggaacac aaccgtagga gaccaccaat cggatttcag acgacgtgta
     2761 gcgcgggctt tgcctgcgga aaactgaaac ccgcctacgg ttccgctttt cagacggcct
     2821 gttgaaagat gcgagagaga aggttgtctg aaaaaccagc cgcaacgtct gccgtcattc
     2881 ccacccccgc ctaagcgagg acaggtcgcg gcgggaatgg cggaggcagc gcggcaactt
     2941 ttttcacggt tctgccaaaa tatgctatga aagaatgttc gtcagcataa tggagatgaa
     3001 gccgatatta taactaatac ggagaattgt gggatatgta ccataagaaa tttgataaag
     3061 aaattctttt aaaaattaaa gaattatata gatacgataa ttggcatgct gtacttgcta
     3121 tattatatga ttatttaata ataatttttt ctatattttt atctgagcag tcatattggt
     3181 ttctcccttt aacgatttta ttaataggaa gcaggcaaag ggcgttggca acaattttac
     3241 atgaagcctc tcattctgcc ttaactaaaa ataagaagtt tggacaaatt ttaggtacat
     3301 atttttctgg atatttaatt tttcaaagtt ggaatagtta ttgtgtttct catgtgaaga
     3361 atcatcatcc aaagttaggg actgattcgg atcctgatta taagtattat aaagactcag
     3421 gtatttttca gacatattca agaattgaat ttttctggag attttgtgtc tctcaaattt
     3481 tatgtttgaa attatttagg aattttaaat atttaattaa aaatagatta ttttcggata
     3541 ttacaagaac agagttgttt aagctacttc tttctcaatt aattctattt gttatcggta
     3601 catattttgt agggtataaa ttttatgtaa tttattggtt attaccatat ttaactgcat
     3661 tccagatgat aacttggttt attgagttgt ccgagcatta tccaatgata caaactgcca
     3721 aaaataattt agaagcgtca agaaataggt ttagtcatgc aatagagcat tttttcacgg
     3781 ggatacatgg agaaaattat catctaatac atcatttatt tccagccatt ccattttgga
     3841 atttggaaaa agcacataag atattaatga aagattttga ttatcaagag gtaaataaag
     3901 attttggagg gattttcttt tcgaaaaaca ataatgcacc tatgtggaaa gtattatgga
     3961 gtaaatatga agctgcgtga tttactgatg ggaatattct tggcagtttc tgcggccctt
     4021 ttgaatgcaa ccatcggcat attcagcaag atattgatgg agcagggctt gtctgttcag
     4081 catattgcat ttttgaaaac tttgacaggt ttcgtgttta tcagcatttt gctttgccgt
     4141 accggtttta ccagacagat tgcggatatt tcaagaaaga aagaggcaat tttgccgttg
     4201 ctgttaaaag tagcaatttg tgcttttttc ggaatttata cgttgttttt ctttgaaacc
     4261 acagcttatc aatatggcaa tgctgcgaat gtagtagttg tattaatggc atcggctgcc
     4321 gtatctgcct tgatattgga cagcatactg ttagatgaac gtatttgcat ttcttcagtc
     4381 gtcggtgtgg gtttggcagt attggggatc gcaatgattt cttggactgg agaaggaagt
     4441 ttagggttga ttctgaatgc cgcactggcg ggctcgggct acggttgttt ttccgttttg
     4501 attaagaaat tcggcctaaa cggcggtatt tatttgacac ggatattgat gttttttgga
     4561 agtatttttt tgtttatccc ttcattggaa ggtattgagg atatacattg gcaatggtct
     4621 tttattccgc cactcttggc attgtcttta ttgccgacga ttttaggatt ttattgtaca
     4681 actaaagcat tggattattt gagtgctgcg aaggtacagg taactgaatt ggccgagcca
     4741 ttgtttgctg ccgtactggc ttggttgttt ttgaatgaaa taccggaagg acgcttcttt
     4801 gtcggcgcca ttctgattat tgccggtatt gtgtctatca atgggctgta tcgaccattg
     4861 ttgaagcgaa ttgaataatt tttatttccc aaaatttcag acgacctctt caacaaacgt
     4921 cgtctgaaac cccaaaacca aaaaaataat agatttagga gaaccgacat ggctgccaac
     4981 caacgttacc gcaaaccgct gcccggtacg gatttggaat actacaacgc gcgtgcggcg
     5041 tgtgaggaca tcaagcccgg ctcttacgac aagctgcctt acacgagccg cattttggcg
     5101 gagaatttgg tcaaccgcgc ggacaaagtc gatttgccga cgctgcaaag ctggctgggt
     5161 cagctgattg agggaaaaca ggaaatcgac tttccttggt atccggcgcg ggtggtgtgc
     5221 cacgatattc tggggcagac cgcgttggtg gatttggcag gtctgcgcga tgcgattgcc
     5281 gaaaaaggcg gcgatcctgc caaagtgaat ccggtggtgc aaacccagct catcgtcgac
     5341 cactctctgg ccgttgaatg cggcggctac gatcctgatg ccttccgcaa aaaccgcgaa
     5401 atcgaagaca gacgcaacga agaccgtttc cacttcatca actggacaaa aaccgcattt
     5461 gaaaatgtgg acgtgattcc ggcgggcaac ggcattatgc accaaatcaa tctggaaaaa
     5521 atgtcgcccg tcgtccaagt caaaaacggc gtggcgttcc ccgatacctg cgtcggtact
     5581 gactcacata cgccgcacgt cgattcattg ggagtgattt ccgtgggcgt gggcggattg
     5641 gaagcggaaa ccgtaatgct gggacgcgcg tccatgatgc gcctgcccga tattgtcggc
     5701 gttgagctga acggcaaacg gaaggcgggc attacggcga cggatattgt gttggcactg
     5761 accgagtttc tgcgcaaaga acgcgtggtc ggggcgtttg tcgaattctt cggcgagggc
     5821 gcgagaagcc tgtctatcgg cgaccgcgcg accatttcca acatgacgcc ggagttcggc
     5881 gcgactgccg cgatgttcgc tattgatgag caaaccattg attatttgaa actgaccgga
     5941 cgcgacgacg tgcaggtgaa attggtggaa acctacgcca aaaccgcagg cttgtgggca
     6001 gatgccttga aaaccgccgt ttatccgcgc gttttgaaat ttgatttgag cagcgtaacg
     6061 cgcaatatgg caggtccaag taacccgcat gcccgttttg ccaccgtcga tttggcggcg
     6121 aaagggctgg cgaagcctta cgaagagcct tcagacggac aaatgcccga cggcgcggtc
     6181 atcatcgccg cgattaccag ttgcaccaac acttccaacc cgcgcaacgt tgttgccgcc
     6241 gcgctcttgg cgcgcaacgc caactgcttc gggctgaaac gcaaaccgtg ggtcaaaacc
     6301 tcgtttgccc cgggttcaaa agtagccgaa atctatttga aagaagcagg cctgctgcct
     6361 gaaatggaaa aactcggctt cggtatcgtt gccttcgcat gtaccacctg taacggcatg
     6421 agcggcgcgc tcgacccgaa aatccagcag gaaatcatag accgcgattt gtacgccacc
     6481 gccgtattat caggcaaccg caacttcgac ggccgtgtcc atccgtatgc gaaacaggct
     6541 ttcctcgctt cgcctccgtt ggtcgttgcc tacgcgctgg caggcagtat ccgtttcgat
     6601 attgaaaacg acgtactcgg cgttgcagac ggcaaggaaa tccgcctgaa agacatttgg
     6661 cctgccgatg aagaaatcga tgccgtcgtt gccgaatatg tgaaaccgca gcagttccgc
     6721 gatgtgtatg taccgatgtt cgacaccggc acagcgcaaa aagcacccag tccgctgtac
     6781 gattggcgtc cgatgtccac ctacatccgc cgtccgcctt actgggaagg tgcgctggca
     6841 ggggaacgca cattaagagg tatgcgtccg ctggcgattt tgcccgacaa catcaccacc
     6901 gaccacctct cgccgtccaa tgcgattttg gccgtcagtg ccgcaggcga gtatttggcg
     6961 aaaatgggtt tgcctgaaga agacttcaac tcttacgcaa cccaccgcgg cgaccacttg
     7021 accgcccaac gcgctacctt cgccaatccg aaactgttta acgaaatggt gaaaaacgaa
     7081 gacggcagcg tgtgccaagg ctcgctcgcc cgcgtcgaac ccgaaggcga aaccatgcgc
     7141 atgtgggaag ccatcgaaac ctatatgaac cgcaaacagc cgctcatcat cattgccggt
     7201 gcggactatg gtcaaggctc aagccgcgac tgggctgcaa aaggcgtacg cctcgccggc
     7261 gtagaagcga ttgttgccga aggcttcgag cgtatccacc gcaccaacct tatcggcatg
     7321 ggcgtgttgc cgctgcagtt caaaccgggt accaaccgcc ataccctgca actggacggt
     7381 acggaaacct acgacgttat cggcgaacgt acaccgcgct gcgacctgac cctcgtgatt
     7441 caccgtaaaa acggagaaac cgtcgaagtt ccggttacct gccgcctcga tactgcagaa
     7501 gaagtattgg tatatgaagc cggcggcgtg ttgcaacggt ttgcacagga ttttttggaa
     7561 gggaacgcgg cttagaggtc gtctgaaaag caagacgtag cgtgggtcgg gttcaacatt
     7621 ttgctcattc acgtaattct cgatatggca ggcatctact gtaaatcgtc attcccgcgc
     7681 aggcgggaat ccagaaagtg gaattgagga aaccttattt atccgatgag tttctgtgcg
     7741 gacaaatttg gattcccgcc tgcgcgggaa tgacggggtt taataatctg ccgtatcaca
     7801 acacagtagc cgtagattgt ggcgaacccc gacagtttgc ggaatcaaac ggctttgtcg
     7861 gagtggcagc ctaatgtact tctggaaagt gggtgtagcg tgggctttgc ccgcgaaata
     7921 aaggctgaat tgacatggta tagaggatta acaaaaatcg ggacaaggcg gcgaagccgc
     7981 agacagtaca gatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
     8041 ctctt
//
LOCUS       DAXIEB010000091         7904 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000091 DAXIEB010000000
VERSION     DAXIEB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.091"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..318
                     /locus_tag="ACFOA3_001607"
     CDS             <1..318
                     /locus_tag="ACFOA3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6898590.1"
                     /translation="DTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFYGENHR
                     VDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANTQSVQQPLLVT
                     PSA"
     gene            complement(660..3263)
                     /gene="pepN"
                     /locus_tag="ACFOA3_001608"
     CDS             complement(660..3263)
                     /gene="pepN"
                     /locus_tag="ACFOA3_001608"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6898591.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNIFELTVKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFQFEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAHEALLDTLAAHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(3475..4269)
                     /locus_tag="ACFOA3_001609"
     CDS             complement(3475..4269)
                     /locus_tag="ACFOA3_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6898592.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFVQAWK"
     gene            complement(5019..5888)
                     /locus_tag="ACFOA3_001610"
     CDS             complement(5019..5888)
                     /locus_tag="ACFOA3_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6898593.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGSLAYFLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKMLDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5939..6475)
                     /gene="ruvC"
                     /locus_tag="ACFOA3_001611"
     CDS             complement(5939..6475)
                     /gene="ruvC"
                     /locus_tag="ACFOA3_001611"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6898594.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(6478..6717)
                     /locus_tag="ACFOA3_001612"
     CDS             complement(6478..6717)
                     /locus_tag="ACFOA3_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6898595.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6748..7758)
                     /gene="dusB"
                     /locus_tag="ACFOA3_001613"
     CDS             complement(6748..7758)
                     /gene="dusB"
                     /locus_tag="ACFOA3_001613"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6898596.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1805 a         2048 c         2036 g         2015 t
ORIGIN      
        1 gacactatgc actttaaagg atttaaagca gcagatgttc attttatccg ttccggaagt
       61 gatttggtgc ttagcgcttc tgaacaagac aacgtacgta tttccggatt cttctatggt
      121 gaaaaccatc gtgtagatac atttgtcttt gatgatgcag ctatcagtaa tccagatttt
      181 gccaagtata ttaatgctgg caataatttg gtacagtcta tgtctgtgtt cggttctaat
      241 actgctgcga caggaggaaa tgtggatgcc aatacacaat ccgtacagca gccgttattg
      301 gtaacgccat ctgcataagg agcctaatca cattcatggc ttaaactgaa aaacagcaat
      361 caagtttatt ttgattgctg tttttcttaa tattgggata agggtcgtat tttaattaac
      421 cttaatcggt gcacttctag caatatagtg gattaacaaa aaccagtaca gcgttgcctc
      481 gccttaccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
      541 ccactataat taatatgact ttgcggccgt tttgccattg cgtaataaaa cgatggggaa
      601 gtgatgataa aacgtgtgtg taactatatc agacggcatt gtttttctgt ttgacggcct
      661 caatccaaaa ttttgccgac gatttcgccc acgtctttcg acaatccttc ctgcgcccga
      721 atgcgctgca attcctgttt caccaagttt ttgcggtgcg gctcgagctt gttgcagagg
      781 ttgaacgctt gcaccaaacg ggcggcgacc tgcgggttaa agcggtcgat ttcgatgact
      841 ttgtcggcga tgaagcggta gccgctgcca tcttctgcgt ggaaatgcgg gacgttgcgg
      901 ctgaagctgc cgatgagcga acgggctttg ttggggtttt cgaggctgaa tttcggatgc
      961 tgcaaggcgg tttggacctg ttgcagggtg tcgctgcggc ggcttgagcc gacgagggcg
     1021 aaatatttgt ccatcaccag cgcatcatcc gaaaacttgt cggcaaactg cgccagcagg
     1081 cggttgcgcg tatcgctttc gttgccgttg acggcggaca ggatgcccca ttcgtgggtc
     1141 atgttttgcg ccatttcgcc gtatttctcg gcaacggttt cgatgtgcgc ggggtcggcg
     1201 cgcaggacga aggcgcggca gacgttgcgc agcgtgcgcc agccggcggc ttcggggctg
     1261 tattcgtaac tttggttttc ctgcttcgcc gcctgacggt tcaattcgtg ccatttcggc
     1321 aggaagtggg cggcaagcgt gtccaacaag gcttcgtgcg cctgatggta gcgcaacggg
     1381 tcgatgtttt ccgtgccgtc ccacagctcg gcttcagacg gcacgcccaa aagcagggct
     1441 ttgaaggcgt tgtccaagag gtcgtctgaa atgacttttt cgacggcggc aagcagtttt
     1501 tcgtgtttcg gcaacccgat gccgtctgaa agcgcggcaa ggttggcggc gacggcgcgg
     1561 cggtagagcg tttgggcggc ttcccagcgc gtgaaggcgt cgctgtcgtg ggcgagcagg
     1621 agcagcaggt cgtcgtcgct gtacggatag ttcaggtgca ccggcgcgct gaagccgcgc
     1681 agcagcgagg gaacgacggc ttcggtcacg ccttcgaact ggaaggtttg ttcggcttcg
     1741 gtcagcagca atacggcttc ggtcgcgcgt ttgccctgat agtcgaatgc cgccgcttcg
     1801 ccgttgcggt tcagcagccc gattttgacg ggaatcatca tcggctgctt gtccgccata
     1861 tcgggcgtag gcggcacggt ttgtttgacg gttaactcga aaatattgtt tttcagacgg
     1921 ccttcggctt ccaaaacggg cgtgcccgcc tggctgtacc acaaggcgaa ctggtcgaga
     1981 ttgatgccgt tcgcgtccgc catcgccgcg cggaaatcgt cgcaggtcac ggcctgcccg
     2041 tcgtggcgtt ggaaatagag cttcatacct ttttggaagc cctcttcgcc gagcaaggtg
     2101 tgatacatcc gcaccacttc cgcgcctttt tcataaacgg tcatggtgta gaaattgttc
     2161 atctcctcat atcgggcggg gcgcaccgga tgtgcggtcg gacctgcgtc ttcggggaac
     2221 tggtgctggc gcaaaaggcg gatgttttcg atgcggcgca cggcgcggct ggcgcggtcg
     2281 ccggaaaatt cttggtcgcg gaacacggtc agcccttcct tcagcgaaag ctggaaccag
     2341 tctcggcagg ttacgcggtt gcccgtccag ttgtggaaat attcgtgtcc gaccacggat
     2401 tcgatgcctt caaaatcggt atcggtggcg gtgcggctgt cggcaaggac gaacttggtg
     2461 ttaaagatgt tcaaaccctt gttttccatc gcacccatat tgaaatcgcc cacggcgacg
     2521 accatgaaaa tatccaagtc gtattccaag ccgaagcgcg tttcgtccca cttcatcgcg
     2581 tttttcaagg attccacggc aaagccgacc ttgggcttgt ccgcttcggt ggtgtaaaac
     2641 tcgattttga cgtttctgcc gctcatggtg gtgaaatagt cttccgttac cgccaaatcg
     2701 cccgcaacca aagcaaacag atagctcggt ttggcaaacg ggtcttccca tttcacccaa
     2761 tggcggccgt ctgaaaactc gccgccgtcg attttgttgc cgttagagag caaaacggga
     2821 tagcgttttt tgtccgcgac gatggtggtc gtgaacttgg acatgacatc cggacggtcg
     2881 atatagaacg tgattttgcg gaagccctcc ggctcgcact gggtaaacag gttaccggcg
     2941 gacgcataca gccccatcag cgatttgttt tccgccggca ggatttcggt ttccacttcg
     3001 acggtgaagc gttcggacgg cacgtctgca atcgtcagcg tctcgccttc caacatataa
     3061 tccgccgccg cgccgttgat tttgacggac aagagtttcg ccgaaccgtc caacaccagc
     3121 ggctccccta ccctctgcgg ctcaaccgtc aaacgcgact tcacgacggt ttgcggttcg
     3181 gcaatatcga aatgcaggtc ggtttcgaga atgcggtagg caggcgtttg gtaatctttc
     3241 agataacgga cggttttgct catttttttc tttcaatatt gtttggtttg actggaaaag
     3301 gcttcagaca gcacggatac agcttttaaa gcggattaac aaaaatcagg acaaggcgac
     3361 gaagccacag acagtacaga tagtacgggc tgtaccggtt tgaatttaat ccaccatatg
     3421 ccgtctgaaa ccgaaacggc ggcacaagcg taccgccttc ggacagcagg ttttctattt
     3481 ccacgcttgg acaaattctg accaatgcgg tttatcttct gtttgggcgg aaagatagtt
     3541 tctgatccgc ttgatttcca tttcgtattc gtccgcgtcg aatctgccgc tgaccagaca
     3601 gaaacgcaga tacatcaaat acgtattcac ggcatcggtt tcgcaataat tgcggatttc
     3661 cttcaattta ccggcatgga atgcctccca gaccttgctg ccgtccatac ccaatttacc
     3721 cggaaaaccg cacagtttcg ccatatcgtc cagcggcaca ttcgccctcg gctggtaaag
     3781 cgcgagcaaa tccatcaaat cgcagtggcg ttggtggtaa cggctgatgt agttgttcca
     3841 cttgaaatcg cggctgtcgc cgaaatctcc gtcgcccata tcccaatagc gcgcggcgtt
     3901 gatgccatat atcagggagc ggtaatgcag aaccggcaga tcgaaaccgc cgccgttcca
     3961 actgaccagt tgcggcgtat gtttttcaat caactcgaaa aatttagcaa tgaccacttc
     4021 ctcgccgtca tccatctcgc cgatggtgcc gacatgtact ttatcctgcc cccaacgcat
     4081 acagcacgaa atcgccacaa cctgatgaag atgatgctgc ataaaatctc cgcccgtctg
     4141 agcacggcgt ttttgctggg caaacagcac cacttcgtcg tcgggcagcg aggacggcag
     4201 ctcgtacaat gttcggatac cctggacatc aggtacggtt tcaatatcga aagccaaaat
     4261 cgtattcatg gcagtacctt gcattcaaaa acagacttgc gcctattgtg tcattaaaag
     4321 gcggataaaa aaagaggcaa ccccccacag gattgcccca atacctcaaa tcagagattt
     4381 acgcttcaca aacaatacag gctttcgcct gcggctttac ccgcgtagct caactctacg
     4441 ccggcaaact ttcgtttcac cgtttccaat gaaaccccga ccaatcgcaa gactgaccgg
     4501 aaaatccttt catacggcat ttcctgcctg tcgtgtaatt ccatgtagcg aaatgtacgc
     4561 cattttctac gcttcgccaa gcatttttta caatataaat gtcaaaacat taattttata
     4621 aaattgctga aaatattaaa tatatggatt tttatttttt atatttcaat aaatataaat
     4681 ttaattttga tttatattta aatttaagca taaaatttca aatattaaag gtaatatgaa
     4741 aggcgtatat taaatattta tttataacgc tatgttttta aagaaaatta attttaatat
     4801 attaactaga ttgtctgcat atattcatgg gtttgcggta tttttttcac aacctgcttc
     4861 gaattttccg accaagtctt aaaaatattg tttttgaaat acttaaatag cagcgattat
     4921 aaaatgaaat ctgttcatat aatctgccat ttcgcattta aaaaaacaat caggagtttc
     4981 gactcgaaac gcctgatatg ttttgtaatt ttacgtagtc agtaaaaatc ggggctgcct
     5041 tccggtcggg ttttgaaacg cttgtgcagc caaaaatact gctcgggatg ttcgcgcacg
     5101 cgttcctcga taaaacggtt catgcgctgc gcgtcggctt gcgcatcttc actcggaaag
     5161 gatttccaag cagggtagaa atgcaatgta accgtattgt ctgcctcgcg gacgggaatg
     5221 gcgggtatca cttttgcatt tgcaagcgcg gcaatgcggc tcaatccggt aatcgttgcc
     5281 gtctgaatac cgaagaaatc cacaaaaacc gaatcgttgc gtccgaaatc ctgatcgggc
     5341 agatacagaa acggcgcgct gcttttgcgg aactgtttga cgagggcgcg cagcccttcg
     5401 gtgcgcccga taaggaagac gttgtgatag cggttgcggc ctttcaaaat ctgttcgtcc
     5461 aacatcttgt ttttttgatg agaatacata ctgatcagcg ggacatcctg attaagcgcg
     5521 tacaccgcca tctcgaacgc ggtgaagtgc ggatacagaa tgatgacttt ttcccccgcc
     5581 gccagcgcgt cgtccaaata atgcttattg cggtagcgca ccagcgattt caaacgtccg
     5641 gcaggcgcgt accaatataa accgtattcc aacatcagtt tcgccatgtg tttgaaatgc
     5701 tgtttcaaca cggttttacg cttttcctca ctccattcgg aaaaacattt tgccaaattg
     5761 atttcgccga tacggcggcg cggtttgacc agaaagtagg caagcgaacc cgtcaggtcg
     5821 gcaatcttgt gcagcagcgc aaacggcaga aactgcaaaa catacagtac aaaaaatata
     5881 aatttcatct cgatacacat tttcttttca gacggcaaaa tacaaatgcc gtctgaaact
     5941 attgaaacct gccgcgcttg acctgcatcc ccgaaggatt gagtttggcg gcaagcccgt
     6001 ggttgcgtaa ggcgtgggtc agcgcgacgg caagaccgtc cgccgcatcc gcctgcggcg
     6061 ttcccgaaag ccccagcatc tgcaccacca tatgctgcac ctgttctttc gccgccttgc
     6121 ctttgccgac taccgcctgt ttgacctgca aggccgtgta ttccgaaacg ggcagcttat
     6181 ggctgaccaa tgccgccaat gccgcgcccc tagcctgacc cagcatcagc gtcgatgcag
     6241 gattgacgtt gacgaacacc tgttccactg ccgcctgttg aggcttgtaa acggcaacga
     6301 cttcgccgat atgccgcaca atcacggcaa tcctgtctgc cagagacgca tcggcaggcg
     6361 ttttgatgca gccggaggca acataaaaat gatcccgccc cctgacatcg atgatgccga
     6421 aacccgttac gcggctgccc gggtcaatgc ccaagatacg gacggttgcc gacatattca
     6481 caacaaaccg tgttgaatca gttttttacg caaggtattg cggttcagcc ccagcatcac
     6541 ggaggctttg gactggttgc cgccgcattg ctccatcacg cacaccagca gcggtttttc
     6601 cacctgatgc aagaccatat cgtacacgcc gcaaggttcg gtaccgttca ggtctttgaa
     6661 atattgttcc aaattttgtc tgatacattg ggaaatatcg ggaagggtat ggggcataat
     6721 cgcgctttca aaaggataat cgggtgttca gacggcattt tggcggtagg cgcacgccca
     6781 acggtcggtt tttccggcaa gtctttcaag ataacctgca agcatgtcgt attgcgccgc
     6841 cgcactgtcc aagcggttga tttcacgacg tgtctgttcg ccgtcgggca tttcgtcgat
     6901 gtaccagcct atgtgtttgc gtgcaatgcg cacaccggcg gtctcaccat aaaacgcgtg
     6961 catggcgcgg acgtggttca aaatggcggc actgcattct gccaaactca aggcaggcgg
     7021 taaaacaccg tgttcggcgt aatgtttcaa atcgcggaag aaccacggtc tgccttgcgc
     7081 gccgcgccct atcatgatgc cgtctgcggc ggtttgttta aggacggctt gggctttttg
     7141 cggcgaagta atgtcgccgt tgacccaaac cgggatgttc agacggcatt tggtttcggc
     7201 aatcaggtcg taagccgctt cgcctttgta catttgcgtg cgcgtgcgtc cgtggacggc
     7261 aagggcggca atgccgcaat cttcggcgat tttggcgatg acgggcaggt ttttgtggtc
     7321 gtcgtgccaa cccaaacggg ttttgagggt aacgggtacg cccgccgcct tgaccaccgc
     7381 ttccaaaatg gcggcaacca gcggctcgtt ctgcatcagc gcgctaccgg cttggacatt
     7441 gcagactttt ttagcgggac agcccatgtt gatgtcgata agctgcgccc caaggctgac
     7501 gttgtaacgc gcggcatccg ccatctgctg cggatcgctt ccggcaatct gcacggcaac
     7561 aatgccgcct tcatcggcaa aatcgctgcg gtgcaaggtt tttctagtat ttctgagcgt
     7621 cgggtcgctg gtcagcattt cgcacaccgc ccaacctgcg ccaaaatctc ggcaaagtcg
     7681 gcggaacggt ttgtcggtaa tgcccgccat cggcgcaagt gcgatggggt tgtcgataaa
     7741 atagccgccg atgtgcataa tgggtccgcg tttcaaaaaa gtgcgacatt gtacattttt
     7801 taagcaggat ttccaatctc cggacgcgcc cgcgattggg gcgggcaccg ttttatggca
     7861 taatccgcac acagatttcc tgccccgcca ctcacaggcg ggca
//
LOCUS       DAXIEB010000092         7893 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000092 DAXIEB010000000
VERSION     DAXIEB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.092"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(49..2091)
                     /gene="recG"
                     /locus_tag="ACFOA3_001614"
     CDS             complement(49..2091)
                     /gene="recG"
                     /locus_tag="ACFOA3_001614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6898597.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAIGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPLLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2177..2620)
                     /gene="secB"
                     /locus_tag="ACFOA3_001615"
     CDS             complement(2177..2620)
                     /gene="secB"
                     /locus_tag="ACFOA3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6898598.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2641..2898)
                     /gene="grxC"
                     /locus_tag="ACFOA3_001616"
     CDS             complement(2641..2898)
                     /gene="grxC"
                     /locus_tag="ACFOA3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6898599.1"
                     /translation="MQTVTMYTGPFCPYCTMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3112..4605
                     /gene="rng"
                     /locus_tag="ACFOA3_001617"
     CDS             3112..4605
                     /gene="rng"
                     /locus_tag="ACFOA3_001617"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6898600.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4640..5890)
                     /locus_tag="ACFOA3_001618"
     CDS             complement(4640..5890)
                     /locus_tag="ACFOA3_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6898601.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(5903..6060)
                     /locus_tag="ACFOA3_001619"
                     /pseudo
     CDS             complement(5903..6060)
                     /locus_tag="ACFOA3_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6283..7596)
                     /locus_tag="ACFOA3_001620"
     CDS             complement(6283..7596)
                     /locus_tag="ACFOA3_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6898602.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGVDVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            complement(7701..>7893)
                     /locus_tag="ACFOA3_001621"
     CDS             complement(7701..>7893)
                     /locus_tag="ACFOA3_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898603.1"
                     /translation="IPRVRHAPRSAMIGAVSFESAHRDECPRAVPIPICRLFRIGCRR
                     EMTDGCRNGGRREWEEMTD"
BASE COUNT         1857 a         2337 c         2052 g         1647 t
ORIGIN      
        1 atcagatttc cgttacactt ttttctaata tttcagacgg cattttgctc acacgcccaa
       61 atacccttcc ctgccggaaa gccaccttgc caaatgcgct tcgacgattt cggggttttg
      121 ctcaatcagc aaaggggcgg tttcgcgcgc ctgttccaaa aggtgcaggt cttcttcgag
      181 tttggcgaaa cgcagcatag gcacgccgct ttggcgcgcg ccgagaaact cgcccgggcc
      241 gcggatattg aggtcttggc gggcgatttc gaagccgtcg gtgtgttcgt agatgacttt
      301 cagccgcgct ttggcgagtt cgcccaaggg ttcggcaaac aggaggacgc acacgctttc
      361 cgccgcgccg cgccctaccc gtccgcgtaa ttggtgaagc tgcgccaagc ccatgcgctc
      421 ggcgtgttcg atgaccatca gggcggcatt gggcacatct acgccgactt cgatgacggt
      481 ggtggcgacc aagacgttca gcccccccga agaaaaccgc gccatcactt cggccttttc
      541 ggcggccttc atgcgcccgt gtaccagtcc gatattgagt tcgggcaatg ccgtctgaag
      601 ccgggcgagg gtttcggcgg cggtttgcag ttgcagggtt tcgctttctt caatcaatgg
      661 gcagacccaa tacgcctgcc gcccttttcg gcaagtgccg aggacgaagc cttcgacttc
      721 ggcgcggcgg acgttgttga cgaggcgcgt tttaatcggt gtgcgcccgg gcggcaattc
      781 gtcgatgacg gacacgtcca aatcggcgaa aaaactcatc gcaagcgtgc gcgggatggg
      841 cgtggcggac atcatcagct gatggacttc gcgccctttg tttttgaggg cgaggcgttg
      901 ggcaacgccg aaacggtgct gttcgtccac aatggtcaag cccaaattgt gaaacgccac
      961 gccgtctgaa aacagggcgt gcgtgccgac ggcgattttg acgctgccgt cagccagttt
     1021 ggctttggct tcgtctttgg cttttttacg caaactgcca aaaaggcgga caacttcaat
     1081 gcccaaaggt tcgagccatt gtttaaattt aataaaatgc tgttcggcaa ggatttcagt
     1141 gggcgccatt acagccacct gcgcaccgga ttcgatagcc gtcaaagcag acaaagcagc
     1201 cacaatggtt ttgccgctgc cgacatcgcc ctgcagcagg cggtgcatcg ggtaggtttg
     1261 cgccatatcg cggcagattt cggaaacaac tttttcttgc gcatcggtca gggcaaacgg
     1321 cagggcttgg cgcagggctt gggtcaatgt gccgtcgccg cccaatgccg ccgccgtgcc
     1381 gccgatacgc ttctgtcgcg ccaagcgcat cgaaagctgt tgcgccaaaa gttcgtcgaa
     1441 tttgagccgt tgccatgcag gcagcgtgcc gtctgaaagc tgatgaatcg tgaaactcgg
     1501 cggcggcgaa tgcaaaagac gcaggctttc ggcgaggtgt ggcagcttca gacggcacag
     1561 cagggcatcg ggcagcgtgt cgtgcagcgg cgtaacgtcc aacgccgtct gaataatacg
     1621 gcgcaaagtg ggctggttca aaccgtttac ggtcgggtaa accggcgtga ggctttccgc
     1681 caaaccgccg ccctcggcat cgcggatttt gggatgaatc atctcgtcgc cgtaaaagcc
     1741 gtgtttgatt tcgcccacgg cgcggatgcg tttgccgatc gccgtctgtt tctgatggct
     1801 ggcgtaaaag tggatgaagc gcagaaaaag gacgctgccg gagccgtcgg cgatttggac
     1861 aatcagctgc ttgcgcggtt tgaacgttac ttcctgatgg ataacctccc cctcgacctg
     1921 acacggcacg ccaatcggcg cgtccttaat cggcataatg tgcgtctcgt cctcgtaacg
     1981 cagcggcagg tgcaacacca aatcccacgc ggtatggagg ttgagtttgt cgagcttctt
     2041 ggcggaaaca tcggtgattt tgagctgttt tcgggtttcg ggcgacatca taggcagatt
     2101 cctttggacg cgcctatttt atccgaaaac aaaaatgccg tctgaaacgg attcagacgg
     2161 catcgacagg caggaatcaa gctccggcgg cttcggcttc ctgctgttgt tggtaaatcg
     2221 cctcaaaatt aatcggcgcg agcaggacgg gcgggaagcc ggcgcgcgtt accgtaccgg
     2281 aaacggcttc gcgcgcgtaa gggaagagga tgttcggaca cgccacgccg agcagcaggt
     2341 cggcatcttc ttcggggatg ttttccaaac ggaaaatacc gctttgggtt acttcgttca
     2401 aaaacatcgt gcgctcgtta tccaatttgg cggttacggt aacggttaca tccacgttgt
     2461 agtagccgtc ttccagcttt tggctgccgg tggaaacgcg catatccact tcgggctcgc
     2521 cctgttccaa aaagatttgc ggcgcgtgcg gcacttccaa agacaagtct ttgacataca
     2581 gtcgctcgat gctgaatacg ggttgcagtt cttcgctcat tttgttttcc tagttggggg
     2641 ttaagggttc agcagtccgt ccagcccgcc ttcctgctgg aggcggtaga ggtcggtaaa
     2701 tccgccgacg tgcgtttcgc cgatgaaaat ctgcggcacg ctgcgctgtc cggaaagctg
     2761 ctgcatttcg gcaaaggctt cgggacttgc atcgacacgg atttcgtcga tatgtccgac
     2821 acctgccgcg tgcagcagcc ttttcgccat cgtgcagtag gggcaaaacg gacctgtgta
     2881 catggtaacg gtctgcatat tgggtttccg aaagttttgc aatgataatc aatatagggg
     2941 catttccccc gtttggcaag tgcggaacag atgcacgttc aaacggcatg tgcggaatgt
     3001 gtcaaagttt cttttttaaa gtatgataga cattgtgaaa aatatttttg cacccgcgct
     3061 gcgcggcgga acggatgcaa aaatattttt attacatttt caggaaaaac catgttgtca
     3121 ggactcccca tccccaaaga catcgcgcgc ccgcccgaaa cgatattggt caacatcacg
     3181 ccgcaggaaa cgcgcgtagc ggtgttggag gaaaacaata tctgcgagct gcacatcgag
     3241 cgcaacagcg aacacagcct agtcggcaat atctatttgg gcgtggtgcg ccgcgtgctg
     3301 cctgggatgc agagcgcgtt tatcgacatc ggcttggaac gcgcggcgtt tttacacatc
     3361 gtcgatgtcc tcgaacaacg ccgcaacccc gaagaaaccc agcgcatcga acatatgctg
     3421 tttgaagggc agtctgtttt ggtgcaggtc atcaaagacc cgatcaacac caaaggcgcg
     3481 cggctttcca cccaaatctc gctggcgggg cgtttcctcg tccatcttcc gcaagaagac
     3541 cacatcggcg tgtcccaacg catcgaagac gatgccgaac gcagcagcct gcgcgaacgc
     3601 ctcgacaagc tcctgccgga aaatgcctgc cggggctaca tcatccgcac caacgccgaa
     3661 aacgccaccg acgaacagct ccagtccgac atcgactacc tgaccaaagt gtgggaacac
     3721 atccaagaac aggcgaaaat ccggccgccg gaaaccctgc tttatcagga tttgcccttg
     3781 agtttgcgcg tgttgcgcga tatggtcggc tgcgacacgc aaaaaatcct cgtcgattcc
     3841 accgtaaacc acggccgcat gacgcgtttt gccgaacaat acgtccacgg cgcattgggc
     3901 aggatagaat tgttcaaagg cgaacgcccg ctatttgaaa cccacaacgt cgaacaggaa
     3961 atcagccgcg ccctgcaacc gcgcgtcaac ctcaacttcg gcagctacct gattatcgaa
     4021 tccaccgaag cgatgaccac gatagacgtg aacacaggcg gcttcgtcgg cgcacgcaat
     4081 ttcgatgaaa cgattttccg caccaacctc gaagcctgcc acaccatcgc ccgcgaattg
     4141 aggctgcgca acctcggcgg catcatcatc atcgacttca tcgatatggc gcaggaaagc
     4201 caccgcgaag ccgtgttgca ggagcttgcc aaagccctcg ccttcgaccg tacccgcgtt
     4261 accctgcacg gttttaccag cctgggactg gtcgagctga cgcgcaaacg ctcgcgcgaa
     4321 aacttaaacc aagtcctctg cgaaccctgc ccttcctgcc aaggcagagg ccgtctgaaa
     4381 acgccgcaaa ccgtgtgcta cgaaatccag cgcgaaatcg tccgcgaagc gcgccgttac
     4441 gatgccgaaa gtttccgcat cctcgccgcc cccaacgtca tcgatttgtt tttggacgaa
     4501 gaatcgcaat ccttggcaat gctgatagat ttcatcggca aaccgatttc tctggcggtc
     4561 gaaaccgctt acacgcagga acaatacgat atagtgctga tgtaaaaaat gccgtctgaa
     4621 gccttcagac ggcatctgtc tatttcaggg tttccttgtc caacaacgcc cgtatcaaca
     4681 gaccgtgtcc gaaacgtcgg ctgtcggtca gttccaaatg tccgccgtat tttttggcaa
     4741 gcgtgtcggc gatggacaga cccagccccg tcccctgctg ctccgttccc aaaatacggt
     4801 aaaacggatc gaggacgcgg gcgcgttcgg attcgggaat gccgttcccg ttatcttcca
     4861 cccacacggc aagatatttc ccttcgtctg tgaaacccaa atctatcctg ccttcggacg
     4921 gggtataacg tattgcgtta tcaacaaacg ttttaaccaa ggtatagatt tcagtttcat
     4981 cggcaaatat ttcgacatca cctccgaccg ccacaccaat gtcctgacgt ttttccaagg
     5041 caagcggcat cagttcctgc aacacttggc ggaaacggct ttgcagaccg aatgtcgttt
     5101 tcgccaaagg ggtttcgtcc gactgcgaac gcgccagtgc caaaagctgc tcgagcaggt
     5161 gtttgttgcg ccgtatgctt tgctgcaaaa cggcagactg ccgcgccgca tcgggtggca
     5221 gagacatatt gttgagccgt tccgcctgaa gggaaagggc tgtcatcggc gtacgcaatt
     5281 cgtgtgccgc gtcggcgaca aaccgctgac ggtggcggat gtcttcatcc gcacgtttca
     5341 aaagcaagtt gatggcggtt acgaaccctc tgatttcact gggaatattg tccacactca
     5401 aggcagacag gtcattgatt cggcgttgtt cgagactttg cgacaatttg cggacggggc
     5461 gcatggcttt gtgcgtaatc cacacggtca gcagtatcat cagcggcagt gccgccagca
     5521 ggggcaacac gctttgccgt gccgcatccg ccgccaaatc ttcccggtat tcgctttcct
     5581 gcatcacggc aatccgcccc cgctccgtcg tgcggatata gacgcggtaa taatcgtcgt
     5641 ccccgtccgc ctgaagcgtg tgcagaccgt ccgccagatg cgcaggcagg ctgacaacag
     5701 ggtcttcctg ctgcggcatc tgtaccaaaa tacgcgtatc gccgtcgccc tcgggcaaag
     5761 tttcgggttt ggaatcgggg gcgacgtaca atgccgcctg acggagcagg tcgtcctgca
     5821 acgcttccgt ttcgtggaag gtttcgtagt aggaaaacat acctgcaagc attgccagcg
     5881 gaacaaacat ccaaaccgtc cgccccggca aacccaaatc cagcagcatc aagtcataag
     5941 gctgggcagc ggcagccgcg cgccgttttt gacccaatcc accgcatagc cgccgtcttt
     6001 caaacttgcc gacaccgcct ccgcaatcat cgcatcgtct tccaccagca aaacacgcat
     6061 caactttccc tttcagacaa accgtgccta ttctaacacc ccaaaattag ccgcaattta
     6121 gcggccttta cgcttgccgg tatttttcaa aactgcagca caaaaaaacc gcgccggcaa
     6181 ctgccttcag acggcattgg ggcgcgattg caacacacgg gcaggcaggc agagcctgcg
     6241 acagaccaca ggaacgattc gggcttcaga cggcttcgcc gtttacggca gaggcacgat
     6301 tcctgccgct atcgaactgg ccaatatcgc cagcgacaaa ccccacgccc aaaaaaacga
     6361 ataacggatg tgtttgccca tcgacaattt cgccaaaccc aagcccatcc acaaagccgg
     6421 cgaaagcggc gtaacaaaag tgccgacgat actgccgatc aacatcgcat aacctgctgc
     6481 ttcgggcgcc acgcccgcct gcgaggtaat ctgctccaca atcggaaaca gtccgaaata
     6541 ataagcgtcc gtactcaaaa ccaactcaag cggaatgccc aacacaccga tggcaatatg
     6601 cagataaggc agcagcgcgt ccggcaggat atgcacaatg tctttggaaa tcgcgtccaa
     6661 catccccgca cccttcaaaa tccccaaaaa cgtacctgcc gccaaaataa tggacgccat
     6721 catcaccgcg ccgccggcgt gggcataaat ccgctccatc tgttcctgcg ggctgcggta
     6781 attcaaaagc aacgccgccg ttgcagccag cataaataca taacccggcg ggaagatgcc
     6841 cgaaaaaagc aggctcatcg ccgccaaaaa cagcaggaca ttccaccaaa acagtttcgg
     6901 acgcgccaat ttttgttctt cttccgacaa aggcaccggc tttatcaaat ccgccacggc
     6961 gggcaacgcg cccaactccc ggacaatccg ccttttttca cgcacaccca aaagcaggga
     7021 cagcgcaagg ataaacacca caccgataat ttgcaccgtc aacaaaggct tatacaattc
     7081 gcccacatct acgcccaaca cgcttgcaac ccgcccggtc ggtccgcccc acggcagaag
     7141 gttgattagc cccgcactgg aagtcagcag caaaaacagc aggtaaggat tcatatgcag
     7201 acgcttgtaa agcggcaaaa gggcggggac gaccaataaa aacgtcgtcg cacccgcccc
     7261 gtccaactgc gccaccaccg acaccaagac cgtccccaca ctcactgcca cgatattacc
     7321 ccgagtcagc ttaatcaaac cgcctatcat cggacggaac agccccacat cgttcatgat
     7381 tccaaaaaac aaaatggaaa acataaacat aatcacaatc tgcatcaccg atttggtgcc
     7441 gcccgaataa aattctttta attgggatac atcaaacccc gccagcaacg ccccaaacag
     7501 cggcaccaag attaatgcga tgatgggcga cactttttcc gtcagcagca accatacgat
     7561 gaccccgata atcagcagtc cgataaacgt cagcatcatt tctcctttat tttattttaa
     7621 acagaaaacc gaccgtgcag gcaaaaccgc ccacagacgc gggataagcc ctgcattcta
     7681 cttttttatt ttgaaacaag tcaatcggtc atttcctccc attcacgcct gccgccattc
     7741 ctgcatccgt ccgtcatttc acggcggcaa ccgatacgga acaaccggca aatcggtatc
     7801 gggacggcgc gggggcattc atcccggtgc gccgattcaa acgaaaccgc ccctatcatt
     7861 gcggagcgcg gggcgtgccg tacacgtggg att
//
LOCUS       DAXIEB010000093         7596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000093 DAXIEB010000000
VERSION     DAXIEB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.093"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(235..1647)
                     /gene="trkA"
                     /locus_tag="ACFOA3_001622"
     CDS             complement(235..1647)
                     /gene="trkA"
                     /locus_tag="ACFOA3_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6898604.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGEVIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1745..3268)
                     /locus_tag="ACFOA3_001623"
     CDS             complement(1745..3268)
                     /locus_tag="ACFOA3_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6898605.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTSPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGAAACEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(3575..4381)
                     /locus_tag="ACFOA3_001624"
     CDS             complement(3575..4381)
                     /locus_tag="ACFOA3_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6898606.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4433..4684)
                     /locus_tag="ACFOA3_001625"
     CDS             complement(4433..4684)
                     /locus_tag="ACFOA3_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898607.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4784..5545
                     /locus_tag="ACFOA3_001626"
     CDS             4784..5545
                     /locus_tag="ACFOA3_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898608.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5843..7012
                     /gene="metZ"
                     /locus_tag="ACFOA3_001627"
     CDS             5843..7012
                     /gene="metZ"
                     /locus_tag="ACFOA3_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6898609.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLVGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7107..7313
                     /locus_tag="ACFOA3_001628"
     CDS             7107..7313
                     /locus_tag="ACFOA3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6898610.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         1631 a         1871 c         2202 g         1892 t
ORIGIN      
        1 tttgaatatt gccgttgccc gacgttctgg attcccgcaa cttttcgtca ttcccgcgaa
       61 agcgggaatc tagaacctct aaattttcag ataacctttg aatattgccg ctatcccaag
      121 gtctggattc ccgctacgcg ggaatgacgg ggagggcgga tgccgtctga agcccaccta
      181 ccattatttc cgcttccata ccgcttggcg gcaatatgtc cggaatgggg cggtttatcc
      241 gaaaaagccc attttgactt ggatgagttt ttccagttcg ttcaggatgc gccggcgcga
      301 gacgaaaaag atgatgtggt cgccgtcttg gatgacggtt tcggtatggt gtcccataat
      361 gacttcgccg gttccggcgc ggacgacggc ggcaatgtgg cagccttcgg gccatttgat
      421 gccgctgatg cgcctgccga tgatggcgga agtttttttg tcgccgtgtg cgacgacttc
      481 gatggcttcc gccgtgccgc gccggatggg gtggacggca acgatgtcgc cgcgccggat
      541 gtgggcgagt atcgagccga tggtgatgag gtggggggag acgacgatgt cgattttgtt
      601 gccttcgagc aaatcgacgt agcttgagcg gttgacgatg ccgatgacgc gcttcgcgcc
      661 gaggtttttc gccaaaaggg cggacataat gttgctttcg tcgtcgttgg tcagggcgca
      721 gaatacgtcg atttcgtcga tgtattcgtt gtcgagcagg gtttcgtcgg ttgccgaacc
      781 ttgcaggacg agggtgttgt cgaggttttc ggctatccat tcggcacggc gcggccggca
      841 ttcgatgatt ttgacgttgt atgcgtgttc gagctgcttg gcgagacggt agccgatgtt
      901 gccgccgccg gcaatcatga tgcggcgggt gctggtttct ttggggcgca attcgggtat
      961 gaccgcgccg atgttttcgg cggcggcggc aaacaggatt tcgtcgcctt cgatgatgac
     1021 ggtttgcggc gcggggacga tgaggcggtt gttgcggtaa acggcgcaga tttggcagtc
     1081 ggccccgtcg ggcaaatctt gggcgatgtc ggcaatgctg cgtccgacaa gcagtccgcc
     1141 gcgccgcgcc tgtatgatga ccatccgcac gcggtcgtct gcaaaacgta aaacctgcaa
     1201 tgcgcccggg cagtctatca ggccggcaag ctgttcggta acgagctgtt cggggctgat
     1261 ggtttcggtt atgccgaata tggaaaggct gccgttttcg ttgttttcga gcttggggct
     1321 gaggtattcg aggtattcgc tggaacggac gcgcgcgatg cggccgggga tgttgaacag
     1381 gtcggcggca actttgcagg cgacgatgtt ggtttcgtcg ctgcgggaga gcgcgagcag
     1441 caagtcggca tcttccgcgc cggcgcgttc taatgtgaag ggggatgcgc cgttgccgaa
     1501 aacggtttgg acatcgaggc ggctgcctgt ttcctgcaat gctttttcgt cgatgtcgat
     1561 aacggttacg tcgttgttgg gtatggcggc aaggttttgt gcgacggtag aacctacctg
     1621 tccgttgcct aaaatgagga ttttcacggt gtgggtcgcc gagtaatgaa atggtggatt
     1681 gtaccgcaac cgggggcgga aatgccgtct gaaggcagat tttgtgttca gacggcatgg
     1741 tgcctcaaga ttcgacgggg atgatgccga ttttcgcctg ccatttgcgc ggggcggtgg
     1801 cgtggatgga ttcgcctttg ctatctacgg cgacggttac gggcatgtct ttgacttcaa
     1861 attcgtaaat ggcttccatg cccaattcgg ggaacgccaa gactttggaa gatttgatgg
     1921 cttttgccac gagatacgcc gcgccgccga ctgccatgag gtacacggct ttgttgtcgg
     1981 cgatggcttc gcacgccgcc gcgccgcgct cggatttgcc gatcatgccc aagaggtcgg
     2041 tttgttcgag catttggcgg gtgaatttgt ccatgcgggt ggctgtggtc ggacctgccg
     2101 gaccgacgac ttcatcgccg accggatcga cgggaccgac gtagtaaatc aggcggttgg
     2161 tgaaatcgac gggcaattct tcgcctttgt tgagcatatc gacgaggcgt ttgtgtgcgg
     2221 catcgcggcc ggtgaggatt ttgccgttca gcagcaatac gtcgccggtt ttccagcttg
     2281 ccacttcttc tttggtcagc ttatcgacat cgacgcgttt gccgttgtcg gggctgtaag
     2341 tcaaatcggg ccagtcttcg acgcgcggcg atgtgagttc gacagggcct gagccgtcca
     2401 attcgaactc gacgtggcgg gtggcggcgc agttcggaat catggcaatc ggtttggagg
     2461 cggcatgggt cggataatcg aggattttca cgtccaacac ggtggtcagt ccgcccaagc
     2521 cttgtgcgcc gatgcccagc gcgttgactt tttcaaagag ttcgaggcgc agggcttcgg
     2581 tggtggacaa ttctgcgccg gacgcggctt tttcctgcaa ttcttggatg tcgatgtggc
     2641 tcatcaggga ctcttttgcc atcaacacgg ctttttcagg cgtaccgccg atgccgatgc
     2701 ccaagatgcc gggaggacac cagcccgcgc ccatggtcgg aatggttttc aatacccaat
     2761 cgacgatgtt gtcggaaggg ttgagcatgg cgagtttgga tttgttttca gaaccgccgc
     2821 cttttgccgc gcaggttact tcgactttgt cgcccggcac gatgctcata tgaatgacgg
     2881 cgggggtgtt gtctttggtg ttttgacgtt tgccggccgg atcggcgagg acagaggcgc
     2941 gcagggtgtt gccttcccaa gtgtaggcgc ggcgcacgcc ttcgttaacc atttcttcca
     3001 cgctcatgtc cgcatcccac tgcacatcca taccgacttt caaaaacacg gttgcgatac
     3061 ctgtgtcttg gcagatgggg cggttgtttt cggcacacat gcggctgttg accaaaatct
     3121 gcgtcatcgc gtctttggcg gcggggtttt cttccttctg ccacgcttta tagagcgcgt
     3181 cgatgtagtc tttgggatgg tagtagctga tgaattggaa ggcatcgcaa atgctttgga
     3241 taaagtcttc ctgtttgatg acggtcatga ttgtgttcct tttcaaggtt gtggcggtaa
     3301 gtgtctgctg ctgttttaaa caaccttttt gaaggctgtt ttttatcagg ttgaaatagt
     3361 gccagcgttt gtacggtaaa gacaatacat aattctaatc tcttgattat caatttgttt
     3421 taataaaata ctgttttact cggattttga tatttgtaat ctttgcctgt tgctttgcgg
     3481 tagggagaaa tcagcaggca gttcattgta ggccgattga tgaacaatgc cgtctgaaag
     3541 ggcttcagac ggcattgtgt tcggacgggc gggcttattt tgcggcctgt ccgtcttctt
     3601 ttgcaaaata tttggcgtaa atcttgtcgt attcgccgct ttcgcgtact ttttccaacg
     3661 catcgttcag cattttgacg gttgcttcgt cgcctttgcg taccgcgatg ccgtagtgtt
     3721 cggtggtgaa gtcgggcagg gtaacgaagt ccatcccttt ggtcggattg tttttcacat
     3781 aattggcgat gaccgcgctg tcgctgacta cggaatctaa gccgccgttt tccagttctt
     3841 tgataatcag gggaacgttt tcaaagcgcg cgatttttgg gttgtcgttg cccaagagtt
     3901 tggatacgga gaaatcgccc gtgtagccgg ttaccacgcc gactttgttc atgtttttca
     3961 aatcgtcgga agaagatatt tttttgcctt tcggaacgag gacgacttgg gtgatttcaa
     4021 aatacgggtc gctgaagtcc atagactgtt tgcggtcgtc ggtaatggtt acgcccgaca
     4081 tcacaacgtc cgcatcgccg ttgttcaagg cggggaaaag gctgtcccac ggctggtgtt
     4141 tgaattcgat tttaaaattg cccgccttcg ccatcgcgtt catcaaatcc acatcgaaac
     4201 cttcgacatt gcctttcgag tctaaagatt caaagggggc aaactcggcg ttggaagcca
     4261 cgcggtacac tttgtcgggg tttgcggcag gtgcggcggc atctttgccc tgaccgccgc
     4321 aggcagacag cgcgagcgcg gaacaggcaa gggcggcggc aatccatttt ttcatattca
     4381 tcaaaattcc tttcggtaat caaaaagcct aaaaaatcag tttaacataa aattaacgtt
     4441 ccttttcacg gaaaaacgca taagtaacgt cgtggaacaa atccgtatgc gggctgtcct
     4501 catagctcaa ttccgccagt gcgtcttcaa aagcaagctg aatcgactcg accgcgactt
     4561 cggcggtttc ggcgggaagg gcgcggatta gggctttgaa ggcggtggac aggacgtggt
     4621 tctgcatacg cagcacgtcg ttcgcctctt ctagatattc caaacgctgt tcgatgctgc
     4681 ccatgatgta tcctcgcgaa tctgttaaaa tgcgccatta tatcaaaccg cgctttgggc
     4741 gcgggtttgt ctgcctgcaa agattaagaa ccggaagccg aaaatggatg cgaataaaga
     4801 gccgaattgg gaaaaacgtt tggaaaacct gcgtcagacg cgccgtatga aggcagtgcc
     4861 gacttacgcg ccgctgtccc gcccctctga aaccggaacg gacgcgcctg tgccgaaact
     4921 ggacgatgag agccgagccc gtgtgttgaa ggcgtatctg aaaaaatggc aggaggaaca
     4981 caatgccgcc gttgccgatt gcggcgagga agtcgaaacc gacccgatga ttgtccttca
     5041 ggaaaactgg ctcaacgcgc aggcttccct gcaaatgtcg gtttcggaaa aacacatcga
     5101 atcgcggcgg agaatcctgt tcgatgccaa aaaagacagt gccgaatttg aaaccggaca
     5161 gattgaaggc ggggaaaacg ccgatgcggc agaatcgggt acggcggcag acggggttgc
     5221 ggaaaacggc gaaggcggtg cggaagaagc gttgatgccc gtccaaatca atattctgaa
     5281 cccgaaagcc gtcaaccggc gcgaagtgtt ctgcctgtcc gaacaggagc tgaccgaacg
     5341 gctgatcaaa cgcctgcgcc cgcatctgac cgataccgtc aacggcatta tccgtacggc
     5401 ggtgcaaaag cagatggcgg ttttcaccta tcagctccag cagatgctgc acgagcaggc
     5461 gggggcggta gtcgaagacg tgttggaaca cgatgtccgc aaaatcctca acgacatcaa
     5521 gtacgagtta aaatacaaac gctgattgcc atgccgtctg aaagccgttt cagacggcat
     5581 ttgcattctc atccaacttg aaagcctcat agaacaggcg caaaatatta atatagtgga
     5641 ttaacaaaaa tcaggacaag gcgacgaagc tgaagacagt acaaatagta cggaaccgat
     5701 tcacttggtg cttcagcacc ttagagaatc gttctctttg agttaaggcg aggcaacgct
     5761 gtactggttt ttgttaatcc actataaatt aaacaacggg cgcacgctcc gttgccgaac
     5821 ggataaaacc gaggaatcac cgatgagcaa aaaactccat ccgcaaacgc tcgccatacg
     5881 cggcggcaaa gaacaaaccg gataccgcga acacaatcag gcattgttcc tgaccagcag
     5941 tttcatgtgg gacaacgccc aacacgctgc cgatttgttt tcaaaaaaaa tcaaaggctt
     6001 cacttatacc cgtaccgcca acccgaccac cgccgccttt gaaaaacgca tcgccgcttt
     6061 ggaaggtgcg gaacgcgcgg tcgccacttc gaccggcatg tccgcgattc aggcggcgtt
     6121 ttttaccttt ttgcaggcgg gcgaccatgt gatttccagc cgcagcctgt tcggcacgac
     6181 cgtcggcttt atcaataaca tcgttaccaa attcggcatc gaagtgagcc acgtgtcgcc
     6241 gactgatata aacgaatgga aagccgccgt caaagccaac accaaactgc tgtttttgga
     6301 aacgccgtcc aaccccttgg gtgaagtggc cgacttggaa gccttggcgg aattggcgca
     6361 cggcatcggc gcgattttgg tggtggacaa cagcctgctg tcgcccatcg gctctcagcc
     6421 tttgaagcac ggcgcagata tttccgtttc ttccgcaacc aaagccatag acggacacgg
     6481 acgcgtgatg ggcggcgtgt tggtgggttc ggaagaactg atggcgcagg ccgccgttta
     6541 ctgcaactct tgcgggctgg cgatgtcgcc gtttaacgcg tggcagttgt tgagcggcgt
     6601 ggaaaccctg tcgctgcgta tggaaaaaca gttcgacaac gccctgaaaa tcgcgcaatg
     6661 gctgcaagcg cagccgcagg ttcaagccgt gtattacacc ggattgcccg accatccgca
     6721 ggcggcgctt atccgcaaac agcaaaacgg cggtggcatc gtcatcggct ttgaagtagc
     6781 cgaccaagaa gccgcgtgga aagttgtgga cggcgtggaa ctcttttccc gaaccgccaa
     6841 cctcggcgac gtgcgctcga ccatcaccca tccgtggaca accacgcacg gcaggatgca
     6901 gcccgaagaa aaactcgccg ccaacatccg ccccggattg gtgcgcctgt cggtcggttt
     6961 ggaatatgtt ggcgatttga ttgacgattt gaaacaggca ctcgcgcgct gattgcgtgt
     7021 tcagacggca tcggcggaaa aacaccgcaa atgccgtctg aaacccttat cttatatggc
     7081 atacacgtca acaataggaa aaaaatatgg atgaagcggt ttttgccgat tgggcgttga
     7141 aaatctgcct gaccggtttg attatttttt tgggttttat cgtttggaat ttgggcaagg
     7201 agtctaaagc gggcaaattc ggcatcgcca ttttgttttt ggttttgggc ttgggcgtgt
     7261 tcggttttgt gttcaaagag ttattgatta agtttttggt attgccgaaa taggtttgcc
     7321 gtctgtcaaa atgctgtctg aaatcataaa aagtaatctg caccctaaaa gtttgattta
     7381 accgccaact gattaaggtg cggattttta tgacaaaata aaaatcatct ttccgcagtc
     7441 attcccacgg acgcgaaagt ctgtcttttt gaatcccgga taattttaca tcgtcattcc
     7501 cgcgcaggcg ggaatctagt ccgttcggtt tcagtcattt acgataaatt cctgttgctt
     7561 ttcatttctg gattcccact tccgtgggaa tgacgg
//
LOCUS       DAXIEB010000094         7492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000094 DAXIEB010000000
VERSION     DAXIEB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.094"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="ACFOA3_001629"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="ACFOA3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6898611.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(575..1384)
                     /gene="proC"
                     /locus_tag="ACFOA3_001630"
     CDS             complement(575..1384)
                     /gene="proC"
                     /locus_tag="ACFOA3_001630"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6898612.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLGKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTIWLEDE
                     AQMHGITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAINEGVCACVRRSQEMERQYQ"
     gene            complement(1415..1816)
                     /locus_tag="ACFOA3_001631"
     CDS             complement(1415..1816)
                     /locus_tag="ACFOA3_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6898613.1"
                     /translation="MRAIKHLAMALLLMFTVAGCQLAGWYKCSSLSGWCKPRKPAAID
                     FWDIVGESPPSLEDYEIPLSDGNRSVRVNEYESAQQSYFYRKIGKFEACGLDWRRRDG
                     KPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1821..2507)
                     /locus_tag="ACFOA3_001632"
     CDS             complement(1821..2507)
                     /locus_tag="ACFOA3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6898614.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRG"
     gene            2627..3670
                     /gene="pilT"
                     /locus_tag="ACFOA3_001633"
     CDS             2627..3670
                     /gene="pilT"
                     /locus_tag="ACFOA3_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6898615.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3833..5059
                     /locus_tag="ACFOA3_001634"
     CDS             3833..5059
                     /locus_tag="ACFOA3_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6898616.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRINDLNLVS"
     gene            5177..7327
                     /gene="yccS"
                     /locus_tag="ACFOA3_001635"
     CDS             5177..7327
                     /gene="yccS"
                     /locus_tag="ACFOA3_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6898617.1"
                     /translation="MKALPIKSLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETADDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALNTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1857 a         2504 c         1622 g         1509 t
ORIGIN      
        1 ttgtttgccg ccatcagtcg gcaaactgtt ttttcatcct ctctcggcgt tcttgggact
       61 caacagataa agtggctgtc gggcgtgcca gcagccgctt cagaccgata ggctctcccg
      121 tatcggcaca gaatccataa tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt tcgttcccga tcgcgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gtcgtagcgg
      301 aagcattttc gatgagttca tcttgcattt taaccagcaa ttcacgaaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc cattccaatt caaaatatct tgttctgtca
      421 gctttgccat aatgtatgtc ctctcagttc accgaaaaaa aagcaccaca aagtggcttt
      481 attcctttga tttttataag gcttattgcc taaggcgttt ttgaaaacgc gcatcataac
      541 acgttttgga ttggtttttt ttattttctc tacattattg atattgccgt tccatttcct
      601 gcgaacggcg cacacaggca caaacgcctt catttatggc ttcggcgaca cgatgccgtc
      661 tgaaggcttc cacggcttcg tgagttgtcc cgccttttga cgttacattt ttttgaagct
      721 tctcgaaatc ttcaccagtc tgctcggcaa gggcaaccgc tcctttaaat gttgccagac
      781 tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat ggcggcattt tgcaatgcgt
      841 ccagcagata aaacacataa gccggcccgc tgccgctgat gccggtaatg ccgtgcattt
      901 gcgcttcatc ttccaaccaa atggtcaaac cgacagattt catgattcga tcggcaatct
      961 tgcagtctgt ttccgatact tccgcttcgg catacatacc ggatacgccc agcccgattt
     1021 ttccgggtgt attcggcata acccggacaa tgcggcgtgt tcccccgagg taacggctga
     1081 gcgtatcaac cgacaatcca gctgcgacag aaagcaccaa tgcaccattg gtgcggatat
     1141 ttttgcacgc ggcttccata tcctgcggct tgacggcaag gattaaaaca tcgtcggaat
     1201 gaagctccgg cagggttgcc gaagtttcga cccccaactc ttttcccaaa cgttcgcgtt
     1261 tttccgcacc ccgattggca atatggatgc ggtaaccgcc ttgtttgacc aatccgcccg
     1321 caacggcagc cgccatattg ccgccgccga gaaaataaac attcattatt tctaccctat
     1381 tcaaaattac ctgactaaat tcattcccaa ttttttacca tcggacgcgc tcggacagac
     1441 cgttgcgccg caacccctgc tttttcaaac aatcaaaacc ttcctgtttg aacgtctcaa
     1501 tcaaaggttt gccgtcacgc ctacgccaat ccaacccgca ggcttcaaac ttccctattt
     1561 tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt taccctgacg gaacgattgc
     1621 cgtctgaaag cggtatctcg tagtcctcta aagacggcgg actctcgccg acaatatccc
     1681 aaaaatcgat ggcggcaggt tttctcggct tacaccagcc ggacagggac gaacacttat
     1741 accaacctgc caactgacag ccggctaccg taaacataag caataacgcc attgcaagat
     1801 gttttattgc gcgcattcca tcagcccctt ttcccgaaaa tcgcgctgcc gatacggaca
     1861 tgtgttgcac cgcacgcaat ggcggcaggc atatcgtccg acatccccat agacagcacg
     1921 tccgccttaa cgccagccgc attgaggtcg gcaagcagtt tccgcatcgt ttgaaattgc
     1981 accttcaact ccgtttcact gctgttggct ttggcaacac acatcaagcc gcgtacgacg
     2041 atattcggca gcttcgccac ttcgaccgca agcacgaccg cttcttcggg cgcgacaccg
     2101 tgttttgccg cctcgcccgc aatgttcacc tcgatacaca cctgcaaagg cggcattgag
     2161 gaaggacgtt gcccgctcag ccggacggcg gttttcagac ggcatacggt atgcacccaa
     2221 tgcgcgcgtt cggcgacaaa tttggttttg ttggactgca catcgccgat gacgtgccac
     2281 acgatgtcgg tcaaatccgc caactcttcc gttttgccgt accactcctg aatatagttc
     2341 tcgccgaaat cacgctgtcc ggcggcgtaa acttcgcgga tgccgtctga agggaaagtc
     2401 ttactgacgg caatcaggct gacggaatgc ggctccctgc ccgcctgcag aaccaatttt
     2461 ccgatacggt cggacacctc acaataacgt tcttgcaaca ccgtcataga ttatccccta
     2521 attaaaaatg attaaacagt tgaaacccct ccagtcaggg cggtacaatc aaggttgtta
     2581 gaaccattcc aaccaatcga aacattatac taaacagagc cgcattatgc agattaccga
     2641 cttactcgcc ttcggcgcca aaaacaaagc atccgacctt cacctgagtt cgggcatatc
     2701 ccctatgatt cgggttcacg gcgatatgcg gcgcatcaac cttcccgaaa tgagcgcgga
     2761 agaggtcggt aatatggtaa cttcggtgat gaacgaccac cagcggaaaa tctaccagca
     2821 aaacttggaa gtcgacttct cgtttgaact gcccaacgtc gcccgattcc gcgtcaacgc
     2881 cttcaacatc ggccgcggtc ccgccgccgt attccgcacc attcccagca ccgtcttatc
     2941 gctggaagaa ttgaaagccc cgagcatttt ccaaaaaatc gcagaatcgc cgcgcggcat
     3001 ggttttggtt accggcccta ccggttcggg caaatcgacc acgcttgccg caatgatcaa
     3061 ctacatcaac gaaacccagc cggcacacat cctgaccatc gaagacccga tcgaattcgt
     3121 ccaccaaagc aaaaaatccc tgattaacca gcgcgagctg caccagcaca ccctcagctt
     3181 cgccaacgcg ctgcgttccg cattgcgcga agaccccgac gttatccttg tcggcgagat
     3241 gcgcgaccca gaaaccatcg gcttggcgct gaccgccgcc gaaaccggac acttggtttt
     3301 cggcacgctg cacacgaccg gcgcagcaaa aaccgtcgac cgtattgtgg acgtattccc
     3361 ggcgggagaa aaagaaatgg tgcgctctat gctgtccgaa tcgctgaccg ccgtcatctc
     3421 ccaaaacctg ctgaaaacgc acgacggcaa cggccgtgtc gcctcgcacg aaatcctgat
     3481 tgccaacccc gccgtccgca acctcatccg cgaaaacaaa atcacgcaga ttaactccgt
     3541 cctgcaaacc gggcaggcga gcggtatgca gacaatggac caatcgctgc aatcgctggt
     3601 gcgccaaggg ctgatcgcac cggaagtcgc acgcagacgc gcgcaaaaca gcgaaagtat
     3661 gagtttctga cacacaaccg ccttccggcc ataccggcgg gaaaacaagg cgcaaacacg
     3721 cgaggcggga cgcagcatcc cgcccggcta cctttccgaa caagaagcgt ccgccttcct
     3781 gttgaaacct gccgccgcaa actgcaaggc ttaaaccgaa aagaagctaa cgatgaatac
     3841 cgataacctg cacgacatct tggacgaaat ggttcaagtg tattctcaaa aaaaacaaag
     3901 ccgatccgaa accccggccg aaatcggcgc acacttccac ccgctgctcg accgcctgtg
     3961 cgaaaccgca gaagcacaaa acgcgtccga catccttatc agcaaaggat tcccgccctc
     4021 gttgaaaatc aacagcgcat taaccccgca gccgcaaaag gcgctgacag gcgaggaaac
     4081 cgccgccatc gccgcatcga cgatgaacgc cgaacaatcg gaaatattcc ggcgcgacgg
     4141 cgaaatcaac tactccgtcc agtcgcgcag cggcacgcgc taccgcgcca acgcctacca
     4201 cagccaaggc agcgcaggtt tggttttgcg gcgcatcaac cacgtcatcc cgcaaatgca
     4261 ggaattaggc ctgcccgaaa aactcaaaga cctcgccgtc gcaccgcgcg ggctgctgat
     4321 tatcgtcggg cctaccggtt cgggcaaatc caccacgatg gcgactatgc tcgaacaccg
     4381 caacaaaacc ctgcccagcc atatcgttac catcgaagac ccgattgagt ttatctacaa
     4441 accgcgccgc tgcatcttta cccagcgcga aatcggcgtc gacaccataa actggcagac
     4501 ggcggtacaa aacgctatgc gccaatcccc cgacgtggtc tgcatcggcg aagtccgcag
     4561 cagggaaagt atggaatacg cgatgcagct cgcccaaacc ggccacctgt gcatttttac
     4621 gctccacgcc aacaccgcgc cgcagtcgct cgaacgcata ctcaacttct accccaaaga
     4681 acagcacaac caaatactga tcgacatcgc cctcaacctg accggcatca tctgccaacg
     4741 cctcgccctc aaacaagaca aaacgggcag gacggcggtt gtcgacttgc tcatcaacac
     4801 gcccgccatc caagacttca tcctgaaggg cgacctgatg aacatcagta aaatcatgga
     4861 aaccgccaaa accgacggaa tgcagacgat ggatcaaaac cttttcgaac tgtaccgtca
     4921 cggcatcatc agttacgaag aagccctgcg ccagtccgtt tccgccaaca acctgcgatt
     4981 gcacatccaa ctgcacaaag aaggcaaaac gcccgaactc ctttacgaca ggattaacga
     5041 cctcaacctc gtttcctaat tgaagaaaca cccgatgccg tctgaaaacc cctgccgttt
     5101 tcagacggca tcgcttcatc atccgcatcc gtgtgctaca cttcatttac ctcataacaa
     5161 taaatccgac aaacccatga aagcacttcc gatcaaatca ctcctcatca cctcgcttcc
     5221 cgttttcgcc agtgttttta ccgccgcctc catcgtctgg cagctaggcg aacccaagct
     5281 cgccatgccc ttcgtactcg gcatcatcgc cggcggcctt gtcgatttgg acaaccgcct
     5341 gaccggacgg ctgaaaaaca tcatcgccac cgtcgccctg tttaccctct cctcgctcac
     5401 ggcacaaagt accctcggca cagggctgcc cttcatcctc gccatgaccc tgatgacctt
     5461 cggcttcacc attttaggcg cggtcgggct gaaataccgc accttcgcct tcggcgcact
     5521 cgccgtcgcc acctacacca cacttaccta cacccccgaa acctactggc tgaccaaccc
     5581 cttcatgatt ttatgcggca ccgtactgta cagcaccgcc atcatcctgt tccaaatcat
     5641 cctgccccac cgccccgtcc aagaaagcgt cgccaacgcc tacgacgcac tcggcggcta
     5701 cctcgaagct aaagccgact ttttcgatcc cgacgaagcc gaatggatag gcaaccgcca
     5761 catcgacctc gccatgagca acaccggcgt catcaccgcc ttcaaccaat gccgttccgc
     5821 cctgttttac cgccttcgcg gcaaacaccg ccacccgcgc accgccaaaa tgctgcgtta
     5881 ctactttgcc gcccaagaca tacacgaacg catcagctcc gcccacgtcg attatcagga
     5941 aatgtccgaa aaattcaaaa acaccgacat catcttccgc atccaccgcc tgctcgaaat
     6001 gcagggacaa gcctgccgca acaccgccca agccctgcgc gcaagcaaag actacgttta
     6061 cagcaaacgc ctcggccgcg ccatcgaagg ctgccgccaa tcgctgcgcc tcctttcaga
     6121 cagcaacgac agtcccgaca tccgccacct gcgccgcctt ctcgacaacc tcggcagcgt
     6181 cgaccagcag ttccgccaac tccagcacaa cggcctgcag gcagaaaacg accgcatggg
     6241 cgacacccgc atcgccgccc tcgaaaccgg cagcctcaaa aacacctggc aggcaatccg
     6301 tcctcagcta aacctcgaat caggcgtatt ccgccatgcc gtccgcctgt ccctcgtcgt
     6361 tgccgccgcc tgcaccatcg tcgaagccct caacctcaac ctcggctact ggatactact
     6421 gaccgccctt ttcgtctgcc aacccaacta caccgccacc aaaagccgcg tccgccagcg
     6481 catcgccggc accgtactcg gcgtaatcgt cggctcgctc gtcccctact ttaccccctc
     6541 cgtcgaaacc aagctttgga tcgtcatcgc cagtaccacc ctctttttca tgacccgcac
     6601 ctacaaatac agcttctcga catttttcat caccattcaa gccctgacca gcctctccct
     6661 cgcagggttg gacgtatacg ccgccatgcc cgtacgcatc atcgacacca ttatcggcgc
     6721 atcccttgcc tgggcggcag tcagctacct atggccagac tggaaatacc tcacgctcga
     6781 acgcaccgcc gcccttgccg tatgcagcaa cggcgcctat ctcgaaaaaa tcgccgaacg
     6841 cctcaaaagc ggcgaaacgg ccgacgacgt cgaataccgc gccacccgcc gccgcgccca
     6901 cgaacacacc gccgccctca gcagcaccct ttccgacatg agcagcgaac ccgcaaaatt
     6961 cgccgacagc ctgcaacccg gctttaccct gctcaaaacc ggctacgccc tgaccggcta
     7021 catctccgcc ctcggcgcat accgcagcga aatgcacgaa gaatgcagcc ccgactttac
     7081 cgcacagttc cacctcgccg ccgaacacac cgcccacatc ttccaacacc tgcccgaaac
     7141 cgaacccgac gactttcaga cagcactgaa tacactgcgc ggcgaactcg acaccctccg
     7201 cacccacagc agcggaacac aaagccacat cctcctccaa cagctccaac tcatcgcccg
     7261 gcagctcgaa ccctactacc gcgcctaccg acaaattccg cacaggcagc cccaaaacgc
     7321 agcctgaaaa agtttcggca ttttgtaaga gagagcagat tgtcagacag gttgcaagat
     7381 agtggataaa gctttgcgca gggtaaatgc gtagcaactg aaccgtcatt cccacgaacc
     7441 tacatcccgt cattcccacg aaaacagaaa accaaaaaca gaaacctaaa at
//
LOCUS       DAXIEB010000095         7355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000095 DAXIEB010000000
VERSION     DAXIEB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.095"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            627..1154
                     /locus_tag="ACFOA3_001636"
                     /pseudo
     CDS             627..1154
                     /locus_tag="ACFOA3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation factor family protein"
     gene            1228..1530
                     /locus_tag="ACFOA3_001637"
     CDS             1228..1530
                     /locus_tag="ACFOA3_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898618.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            1877..3508
                     /locus_tag="ACFOA3_001638"
     CDS             1877..3508
                     /locus_tag="ACFOA3_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6898619.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNSCTFDWNGGDCSVNKGDDFRNGANFSLIRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTS
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDI
                     LACDRLPESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            4453..4737
                     /locus_tag="ACFOA3_001639"
     CDS             4453..4737
                     /locus_tag="ACFOA3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6898620.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            4738..5926
                     /locus_tag="ACFOA3_001640"
                     /pseudo
     CDS             4738..5926
                     /locus_tag="ACFOA3_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
     gene            6141..7097
                     /locus_tag="ACFOA3_001641"
     CDS             6141..7097
                     /locus_tag="ACFOA3_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6898621.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2214 a         1528 c         1777 g         1836 t
ORIGIN      
        1 ctgttgttct aaggtctaga ttcccgcctg cgcgggaatg acgggatttg aggttgcagc
       61 atttatcggg agcaacagaa tccgctctgc cgtcattccc acgaaagtgg gaatctagtt
      121 ttttgagttt cagtcatttc cgataaattg ccttagcatt gaatgtctag attcccgcct
      181 tatatgatgc gctctatcaa aggggcgcat taattttctt aacattcccc tttgacagcc
      241 aagtgaaagg ggctttttta tgtcagcagt aaatgtaata ttttcctgtt cttattggag
      301 aatatttaaa aaatcagatt cttgtgtttt gtgtttttat cagttcagac atggcgaacc
      361 gcataaactc attaatcaag agaatttttc aaagctttat caggcgttcg attatataga
      421 ttcggttggt tcgaattttc cagtgattat cacaacggat ggttgtggtc ttttttgttg
      481 atctttaaaa gtttgtcagg atttggcttt cggtcgttga ccgtcgtacg cgctttagcg
      541 cggaaaactg gaaacggctg aaagcccccc ccttgactaa caggggggga gcgaaattaa
      601 aaaccaattc caagagtagt gaacgaatga gtgaagttga atatttctca cactttatat
      661 cggacggaaa agggaagctt ttagaaattc cgcagcgaag aggtaagcaa gacggggttt
      721 ttgttgattg gatttcattc acattccatg aagatacttt actgaaagtt tccggttgcc
      781 ctttattttc tgatgctgaa tacatgtatg tattaagcag aaagctggaa gaaattctag
      841 gttttggcat aacgcgcaaa tgcaaatcaa ggggcaacaa attctatgaa tccatgtata
      901 ggttaggttc ggatgatgtt gattatggag aggtgcattt cggaggtcag cgcaatactg
      961 ttttagttga gttgaaaggt actggttgca gcgttgcaag tccgggttgg gagttgaggc
     1021 taaagcagtt tctcgatgat tcggtaagga caagaataac gcgaattgac ctagcacttg
     1081 atttttttga tggagagtac acgccggatc aggcgttgtt agatcacgat aatggttttt
     1141 ttgataacag caatcaaagg cttcaagatg gcttggagca tgatcaaatc tgtcaaataa
     1201 acagagtgaa gaaaaagggg cgcataaatg ggctatcgtg tcggcataaa ttgttttgat
     1261 acaagattgc aggcagacga ctatttattg tcgtcccttc ctcctactgt tacccaggac
     1321 ggaaaaatca tcaggccgga aagggtgggc gataaatgga ttttgaacgg aaagccggtt
     1381 acgttgtctt atccggaatg ttccaatttt gagcagataa agcaaggttc ttatgtcggt
     1441 tcgacggttc taattctgtt tgtagtcatt tacggtttca ggcttctgat taatttttta
     1501 aaagacatag gcaaggttgg gactgattga tgattataga tttctggttt cttctcggtt
     1561 tcttcttggc tttgtctgtt gcttggctgt tttggtaacg gttggtagaa tcggcttttt
     1621 agagtgtttt aaaaggtccg aattatgttt atttctgaat atcatttagt taaatttcaa
     1681 actgattcac atatttatag agatttacca caagcgttaa tttattatag ggaattgatt
     1741 agaaaagggg tttttaaaac ttcgttttca tttgatattt ttaggaattt ctttcatcgt
     1801 tatgatagag attttataga aattcaattc cctgattctt ctacattatt aattaaatta
     1861 gatgaagcaa aatgttatgt ttattatcct agggcgaaat tttttaaaga ttatcctatg
     1921 ctttagtttt tttgtatcta aatttgcatt ggcatcagta aatgctccgg gtaaatttga
     1981 tagggttgaa gtttatgatg atggcagata tttaggtatt cgaggttcag atgacaaaag
     2041 aagaagaatt tggaaaggtg tatttgatag agaatcggga agatatttaa cttcagaagc
     2101 tcaagattta aaagttaggc atgtatctac tggagcatca agtacgggta aagttagttc
     2161 ggttgtatct tcatcagttt cccgcgctgg cgtattggcg ggggtcggca aacttgcccg
     2221 cttaggcgcg aaattaagca caagggcggt tccctatgtc ggaacagccc ttttagccca
     2281 tgacgtatac gaaactttca aagaagacat acaggcacga ggctaccaat acgacaccga
     2341 aaccgacaaa tttgtaaaag gctacgaata tagtaattgc ctttggtacg aagacgaaag
     2401 acgtattaat agaacctatg gctgctacgg cgttgacagt tcgattatgc gccttatgtc
     2461 cgattacagc agattccccg aagttaaaga attgatggaa agccaaatgt ataggctggc
     2521 acgtccgttt tggaattggc ataaagaaga actgaataaa ttaagttctt tggattggaa
     2581 taattttgtt ttaaatagtt gcacatttga ttggaacggc ggagattgtt cggtcaataa
     2641 aggtgatgat ttcagaaatg gggctaattt ttcccttatc cgcaatccga aatacaaaga
     2701 agaaatggat gccaaaaagc tggaagagat tttatcgttg aaagtcgatg ccaatcccga
     2761 caaatacata aaggcaaccg gttatcccgg ttattccgaa aaagtagaag tcgcacccgg
     2821 aacaaaagtg aatatgggtc ccgtcacgga caggaacggg aatcccgttc aggttgtcgc
     2881 aacattcggc agggattcgc aaggcaacac cacggtggat gttcaagtaa tcccgcgtcc
     2941 cgacttgacc cccggaagcg cggaagcacc gaacgcacag ccgctgcccg aagtatcgcc
     3001 cgccgaaaac cccgcaaaca acccgaaccc caatgagaac cccggcacga gccccaatcc
     3061 cgaacccgac cccgatttga atcccgatgc aaatcccgat acggacggac agcccggcac
     3121 aagacccgat tcccccgccg ttccgggacg cacaaacggc agggacggca aagacggaaa
     3181 ggacggcaaa gatggcggcc ttttgtgcaa attcttcccc gacattctcg cttgcgacag
     3241 gctgcccgag tccaatccgg cagaagattt aaatctgccg tctgaaaccg tcaatgtaga
     3301 gtttcagaaa tcaggaatct ttcaagattc cgcacagtgt cccgcacctg tcactttcac
     3361 agtgactgtg cttgattcaa gcaggcagtt cgcgttcagc tttgagaacg catgtaccat
     3421 agccgaacgg ctaaggtaca tgcttctcgc ccttgcttgg gcggttgccg cctttttttg
     3481 tatccgcaca gtatctcgtg aagtctagca ggcgcagcac cgccgggctt cagtaacttg
     3541 taccaaggca gggggaggac gtccagaaag atttgtaaag acggctttat cgtctttata
     3601 aatctttttg gatacccctt gccgccccgc caaaagaaca cattctgccg caagggcagg
     3661 tggtaaggcg cgcgcctttt gcgccgttcc ccctgccccc gcggcgtcgc aagtgagact
     3721 gggggtgcgg gggctagtcc ccgcaaagcc tttcagcttc ggaagccacg gccgaaaggc
     3781 aggcgcagca ctgccggtct gagcggaagc caggctacag gcaggcgcag caccgccgag
     3841 ctaggcggaa gccaggctac aggcaggcga agcaccgccg gttgggcgga agccacggcc
     3901 gaaaggcagg gcgaagcacc gccaggctta ggcggaagcc acggccgaaa ggcaggcgaa
     3961 gtaccgccgg tctgggcgga agccatggta aaaggcaggc gaagcaccgc cgggcttcag
     4021 taacctttgt tcaggcaggg ggaggatgtc cgtaaagaat cgtaaaggcg gggttttttc
     4081 gcctttatga ttctttatgg ataccccttg ccgctcggac aaagaaatac actctgccgc
     4141 aagggcaggt ggtaaggcgc gcgccttttg cgccgtcccc ctgcccccgc ggcgtcgcaa
     4201 gtgagactag ggggtgtggg ggactagtcc cccgcaaagc gttcagcttc ggaaactttg
     4261 gccgaaaggc aggcgaagca gcgcactttg cgacgaatgt cgcaaatagc cgagaagcgc
     4321 ggggggattg gcgataagcg cgaggggggt gtccccacag cgccgccgcg ccgcgaatgc
     4381 ggcgcaaaat ctttcagatt aagaaacatt tgtttaatga ggcaaccgtg ccttttaaga
     4441 aagggatagc aaatgaaatt gttggccgca ttgattccgc ttttgatgag cgtggcaggc
     4501 cgtatattga ctgcattagg cttgatggcg gtaacctatt caggggtgga tagattggta
     4561 gcccattttc agcaggcgat aaccaatagc ataacgggcg cgcctcaagc gatgttgcag
     4621 cttttttata taagcggcgg tggaaccgtt cttaatatcc tgtttggtgc gatcgccttt
     4681 attctgtcat ttaaacaaat gacaaaacta gcaacctcaa tcgggaagaa aaaataaatg
     4741 gcagagatct gtttgataac aggcacgcct ggttcaggga aaacattaaa aatggtttcc
     4801 atgatggcag gcgatgaaat gtttaagcct gatgaaaacg gcatacgccg taaagtattt
     4861 acgaacatca aaggcttgaa gataccgcac acctacatag aaacggacgc gaaaaagttg
     4921 ccgaaatcga cagatgagca gctttcggcg catgatatgt acgaatggat aaagaagccc
     4981 gaaaatatcg ggtctattgt cattgtagat gaagctcaag acgtatggcc ggcacgctcg
     5041 gcaggttcaa aaatccctga aaatgtccaa tggctgaata cgcacagaca tcagggtatt
     5101 gatatatttg ttttgactca aggccctaag cttctagatc aaaatcttag aacgcttgta
     5161 cggaaacatt accacatcgc ttcaaacaag atgggtatgc gtacgctttt agaatggaaa
     5221 atatgcgcgg acgatcccgt aaaaatggca tcaagcgcat tctccagtat ctatacactg
     5281 gataaaaaag tttatgactt gtacgaatca gcggaagttc ataccgtaaa taaggtcaag
     5341 cggtcaaagt ggttttacac tctgccagta atagtattgc tgattcccgt gtttgtcggc
     5401 ctgtcctata aaatgttgag cagttacgga aaaaaaacag gaagaacccg cagcacaaga
     5461 atcggcggca acagaacagc aggcagtact tccggataaa acagaaggcg agccggtaaa
     5521 taacggcaac cttaccgcag atatgtttgt cccgacattg acggaaaaac ccgaaagcaa
     5581 gccgatttat aacggtgtaa ggcaggtaaa aacctttgaa tatatagcag gttgtgtaca
     5641 aggcggaaga accggatgca cctgctattc gcatcaaggg acagcattga aagaagtgac
     5701 ggagttgatg tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga
     5761 aagccaaggg caggaagttc ggcaaagcgc acagcaacat tcggacaggg cgcaagttgc
     5821 cacgttgggc ggaaagccgc tgcaaaatct tatgtatgat aattggcagg agcgaggaaa
     5881 accgtttgaa ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaaga
     5941 gagaaaaaag acccataaac cgtttgaata tagacggctt acgggtcttt gtttcgcgca
     6001 aagcaaaggc taaggcagtc aggcagcaaa tcccgcaatg tatcaaaaca gacgcgtaga
     6061 aatgccggct gcctttatcc atcctcaaaa ttgaatatca tcctagccgt atcaaggctg
     6121 tataaataag gaaaatacca atgaatataa tcgggctgga catctcaaag gacaccatag
     6181 acgcaacatt gcataaaaca aacggaagta tccattacat taaatttaag aataatgatg
     6241 atggattaaa acagtttaga ttgtggataa agggaaacag aatcagaaaa gcctatatcg
     6301 gcatggaggc aacaggcatc tattacgaaa aggcagcaga tatgctttct tcctactata
     6361 ctgtttacgt tattaatccc ttaaaaatca aggactacgg aaaaagcagg tttaaccgta
     6421 ccaaaaccga caaagcagat tcaaacctga tagcagacta cataaaaagg catcaagata
     6481 cattgatacc gtatcagata cccaaaaaca aagcactgca aaaactgatt aaccttaaaa
     6541 atcaattaca tcaacatcag aagcaaatta aaaaccgtct tcatagcact gaagaagact
     6601 tcataaggaa catacatcaa gacttgatag ataccataca ggacaagatg gaacaggtaa
     6661 aaatagccat atccgaacaa atcaaaaaac aaacggacaa taaccattac cgcaatcttc
     6721 aaaccatccc gagcataggc aaagacaccg catcagttct ttatgcgcaa ctgacagaaa
     6781 aacattttaa aaccgcaaac cagtttgtat cctatgccgg attaagtccc gccatcatac
     6841 aatcagggac aagcgtaaga ggtcggggca gattgagccg atacggaaac agacgattaa
     6901 aaagtacgct gtatatgccc gccctttgtg cttaccgttt taacgcattt ccgaaattaa
     6961 taaataatct gaaaaaagcg ggtaagccaa agatggtaat catcgttgcc atcatgcgca
     7021 aactggcgaa gctcgcctat tacattgtta aaaccggcca gccttacgat gcggaaagac
     7081 accgattgaa tcaataaaat tcaacaaaat taaacggtta cgcgaatata tttgtgtaac
     7141 cgtgcatttg catatcgtaa ataaacgtaa ataaaaataa caatataaat cagtatattg
     7201 caactttgtt ttttattttg tgttgacggg caacatatca tctgcgcggg aatgacgaat
     7261 ccatccatac ggaaacctgc accacgtcat tcccacgaaa gtgggaatct aggacgcaaa
     7321 atctcaagaa accgttttac ccgataagtt tccgt
//
LOCUS       DAXIEB010000096         7156 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000096 DAXIEB010000000
VERSION     DAXIEB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.096"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(52..807)
                     /locus_tag="ACFOA3_001642"
     CDS             complement(52..807)
                     /locus_tag="ACFOA3_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6898622.1"
                     /translation="MKYVRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(932..2077)
                     /gene="nirK"
                     /locus_tag="ACFOA3_001643"
     CDS             complement(932..2077)
                     /gene="nirK"
                     /locus_tag="ACFOA3_001643"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6898623.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAASAEAASSAAQAAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAKSDY"
     gene            2448..4703
                     /locus_tag="ACFOA3_001644"
     CDS             2448..4703
                     /locus_tag="ACFOA3_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6898624.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4882..5415
                     /locus_tag="ACFOA3_001645"
     CDS             4882..5415
                     /locus_tag="ACFOA3_001645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6898625.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5478..6665)
                     /locus_tag="ACFOA3_001646"
     CDS             complement(5478..6665)
                     /locus_tag="ACFOA3_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6898626.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVYPKPPRHSNSADNLLRIVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVAD
                     ISE"
     gene            complement(6705..7109)
                     /locus_tag="ACFOA3_001647"
     CDS             complement(6705..7109)
                     /locus_tag="ACFOA3_001647"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6898627.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1596 a         1734 c         1998 g         1828 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct
       61 tgtgcaacgg aagcccaagt tgtgcaagac atatttggat tgcaggctgg tacggatgcc
      121 gtagcggagg aaggcggcat agttggacga gtcgctcgac ccgatagacg cgccgctgca
      181 aaacatttgc gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca
      241 ttcccaaatc agcccgtgca tatcataaac gccccagtag ttcgggcggc ctttgccgac
      301 atcgtgcagg cctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc
      361 gggttcgttt gagccgtttt tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc
      421 aatggtcggc aggcgtttgc cttgtgcggc gcaataggcg ttggcggcaa accaggaaac
      481 attggttacc ggttgtttta attcgcccgc cttcggcgca tagctgcggc tgccgttttt
      541 catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca
      601 ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag
      661 tttgaacggt ttgactttaa tcaggccggt atcttttttc agataaagcg ggcggtagct
      721 gccgccttcg atttgaacca tttcggcagc cgccgcttga gtgccggcga gtgccgcaca
      781 gagaaagagt aaccggacat acttcataaa gcctcctgac aggcggttaa aatcaatctt
      841 ccgaaaggaa agattggttg ttaaaaaacc actgccgtgc gtaatgaagt acggcggcag
      901 tggttcgttt gcctaaaaat gggtgttcaa tttagtaatc gctttttgca ggagcagaag
      961 ctgcgggagc ggaagcagca ggagctgcac cgttaccggc gtaagcggta tcactcaatt
     1021 tttgagtcat gatttcaggg ttttctgcac cttctacttt caattgaccc agtgcgcctt
     1081 tgttgaatgc gcggaagata gagtggtcaa ccaaagtgta gctgcccggg atgtcgactt
     1141 tgaattcgac gatggcagag ccgccggcag gaacgatggt actttgtacg ttttcgttaa
     1201 tcagtttgcc gccttcaaca taaactttgt cgaagatttc accgattacg tggaaggaag
     1261 acaccaagtt cgggccgccg ttaccgacgt acatacgcac ggtttcgcct gctttggctt
     1321 tcagcgcgtt atcgccggcg atagaaccta catgaccgtt gaatacgacg tattcaggct
     1381 gttcggcaac ggctttgtcc atatcgaacg gttgcagacc ttgcgcgcct tttttgcctt
     1441 tggtgtagaa gtcgccttgg acgatgtaga actctttatc cactttcggc agaccttctt
     1501 taggctcgac caaaatcaga ccgtacatac cgttggcgat gtgcataccg accggcgcga
     1561 cggcgcagtg gtagatgtac aggccaggtt gcagggcttt gaagctgaat gtggaagtac
     1621 ggcccggagc ggtaaaggtt gcggccgcgc cgccaccttg gccggtagct gcatggaagt
     1681 caacgttatg gggaacggta gaagaaggat tgttagagaa ttctacttct acggtatcac
     1741 cttcgcgtac gcgaatcata cggcccggaa cgtcgccgtc aaatgtccag tagcggtatt
     1801 ccaccccgtc gtccattttc atggtttttt cgacggtttc catttttacg cgtactttgg
     1861 caggatagtc gcggtcgatt gcgggaggta cttcaggagc gtgggtggta actgcatcga
     1921 tgacgggcag ttcgcctgca ggcgtttcgg cggcagcttg tgcggcggaa cttgcggctt
     1981 ctgcggaagc ggcaggggct tgcgcggcag gttcgccgcc gcaggcggct aatgcgaata
     2041 aggaagcaat cattgcagct aaggcttggc gtttcataat gttttccttt tgtaagaaaa
     2101 gtagaggaat tggttaagaa cttggtgtga ttatgcgttt gccccattgg gggcattttg
     2161 atttaagtca aataattcat aaaatatgaa cttaaaatga tacttctaat actttatagc
     2221 tactaaaaat tacaataaat aggctgattt gcttaagggc ttcggcaaat caggcaaagg
     2281 cagggtagtt gccgactaaa gaataatgat agttattatc atatatgtat ttgttttata
     2341 tgtgagtttc tttgaacaaa tgtttatttg cgcggtaaac cgtgctacaa tctttaacat
     2401 tcatattttg tgaattttaa tccactatat taaaaggagc tctcaaaatg ggacagtaca
     2461 agaagctgtg gtacttgctg tttgccgttc tggcggtatg ctttaccatt cttggctata
     2521 tgggcagcga ggtttataag aaagccccgc cttaccccga acaggtcgtt tccgcatcag
     2581 gcaaagtgct gatgacgaaa gacgatattt tggcaggtca gtctgcgtgg cagagtaccg
     2641 gcggtatgga agtcggttcg attctgggtc acggcgcata tcaggctccg gactggacgg
     2701 cggactggct gcatcgtgag ttgtccgctt ggttggattt gaccgcgcaa cagacttacg
     2761 gcaaaaaatt cgatgaagtt tcccctgaag aacaagccgt tctgaaaacc cgactggctg
     2821 acgaataccg caaccaaagc cgtattaaag aagacggcag cgtcgtcatc agcgatacgc
     2881 gcgtgaaagc catcgaaagc atcctgcctt attaccacgg tgtttacggc gatgatcccg
     2941 cgctgcaaac cacccgcgaa cactttgcaa tgaaaaacaa cacattgcca agccaagaag
     3001 cgcgtgaaaa actgttcgac ttcttcttct ggacttcttg gtctgcttcg accaaccgtc
     3061 ctgacgagac gttcacttat accaacaact ggccgcacga gcctttgatc aacaacgtac
     3121 cgaccactga aaactatatg tggtcattca ccagcgttgt tcttctgctg atgggcatcg
     3181 gtttgctgat gtggggttat tccttcttga ccaaacacga ggaagtggaa gtgccgtctg
     3241 aagacccgat ttccaaaatc caactgaccc cttcgcaaaa agcattgggc aaatacgtct
     3301 tcctgacggt cgccctgttt gtggtacaag tattgctggg cggactgacc gcgcactaca
     3361 ccgtcgaagg ccagggtttc tacggcatcg acgaagcgtt gggctttgaa atgtccgact
     3421 ggttccctta tgccctgacc cgtacttggc acatccaatc agccatcttc tggattgcaa
     3481 ccggcttttt gacggcaggt ttgttcctcg cgccgattgt caacggcggt aaagatccca
     3541 agttccaacg tgccggcgta aacttcctct atatcgccct cttcatcgtg gtcggcggtt
     3601 cttacgcggg caacttcttc gcgttgacgc acatccttcc cccggaattt aacttctggt
     3661 tcggacacca aggttacgaa tacctcgatt tgggacgttt ctggcaactc ctgctgatgg
     3721 tcggcctgct gttgtggctg ttcctgatgt tgcgttgcac ggtttccgcc ttcaaagaaa
     3781 aaggcgtgga caaaaacctg ctggcaatct ttgtcgcttc tatggtcggt gtgggcgtat
     3841 tctacgcacc gggactgttc tatggcgaga aatccccgat tgccgtaatg gaatactggc
     3901 gttggtgggt ggttcacctg tgggtggaag gcttctttga agtatttgcc actgccgcct
     3961 tcgcatttgt cttctacaat atgggctttg tccgccgcag taccgccaca gcttctactc
     4021 tggccgctgc cgccatcttt atgttgggcg gcgttccggg tacgctgcac cacctgtact
     4081 tctccggctc tacctccgcc tctatggcaa tcggtgcctg cttctccgct ttggaagtcg
     4141 tgccgctggt gttgctgggc cgtgaagcat acgagcattg gtcttaccaa cacctgtccg
     4201 actgggcgaa acgcctgcgt tggccgctga tgtgcttcgt ggcagtcgcc ttttggaata
     4261 tgatcggtgc cggtgtattc ggtttcctga ttaacccgcc gatttccctg ttctatatcc
     4321 aaggtttgaa tacttctgct gttcatgcgc acgccgcctt gttcggtgtg tacggtttct
     4381 tggcattggg cttcgtattg ctggttgccc gttatttgaa accgaacgcg cagtttgacg
     4441 acaaactgat gacttggggc ttctggctgc tcaacggcgg cttggtcggt atgattgcca
     4501 tcagcctgtt gccggttggc gtgattcagg catacgcctc catcacgcac ggcctgtggt
     4561 atgcacgcag cgaagagttc ctgcaaatgg aaatcctcga caccttgcgt tgggtacgta
     4621 cggcagctga cttggtcttt atcggcggtg caatctgcgt tgccatccaa gccactaaaa
     4681 tcgtattcgg ccgcgacaaa taagttttgc ggtatgaaat gagcaactgc acccgttttc
     4741 gggtgcagtt ttttattgcg gattgggacg ggaatggaaa aggatgtgga ttttttattt
     4801 taatcggcta tacaattgct gctttcatgc cgtctgaagc acgtttcaga tggcatcggt
     4861 ttacatcaga taaggataaa aatgggtatt tacgattttc aaatgaaaga tgcggaaggc
     4921 aatgcggttg atttgtcagg ctatcgcggc aaggttctgc tgattgtcaa cacggcaacg
     4981 cgttgcggtc tgaccccgca atacgaggct ttgcagaagc tgtatgcaca atataccgca
     5041 gaaggcttgg agattttgga ttttccgtgc aaccagttcc gcgaacaggc tcccgaaagc
     5101 agcggagaaa ttgcccaagt gtgtatgatg aagtttggca cgaagtttaa gattttcgac
     5161 aaaatcgaag tcaatggagc aaataccgcg cccttgtatg cctacctgaa atccgtcaaa
     5221 ccgcaagata aaggcaatca tctgtttaaa gatttcgtgt tgaagctcgc agctttggga
     5281 gagaagcgcg acgaaggcga catcaaatgg aactttacca aattcctcgt caaccgcgac
     5341 ggcgaagtgg tcgagcgttt tgcgcctagt gttacgcccg aagaaatcga agccgatatt
     5401 cgggcattgc tgtaatctgc cgccgtcttg ggaaatgccg tctgaaggct tcagacggca
     5461 tttgtccgtt tgcgggttta ttccgaaata tcggcaacac cgctttcggg gaagatgggc
     5521 aaatccggcg gtgggagcag ggtatccaaa gtcagggcgg acgcatcgag tgtcgggtag
     5581 ccttcaaacg cgaccgaata accgctgttt ccgttcatac ggattcgggc gtgcgcgcct
     5641 agagggaaag tgattttgtc ggcaatgtgt ccgaacggga agcccgtcag cacggggatt
     5701 ttcgccgtgc gcgaaacatg gttggcaacg gcggaaaaat cataagacgg atcatagaca
     5761 tctcgaattt tttccatacg gaaattgccg aacacgatgg cgcgctgttt cttcaaaata
     5821 cccgaaagat acagcgtatt gagcatacgt tcgatgcggt agggctgttc gccgacatct
     5881 tcgaggaaca aaatgccgcc gtcgatgtcg ggcatataag gcgtgccggc gagcgaggcg
     5941 aggacgctta agttgccgcc ccacaatatg ccttcggttt cgacatcggc gcgttggata
     6001 taaggaacat caacggtcag gcggttttgg gttgcaccct tgataaaggc atccatcgta
     6061 aacgcaccgg gggcgggttt gccaaaatcg ctataagcca tcgggccggc aaaactcatc
     6121 atattgcctt ttgccaacaa tgccagctgg acggcgcata cgtcgctgaa tccgaaaaac
     6181 agcgtgccgt gttcgcgcat ccttgcgccg agcgaagcaa aatcgatatg cggcagaatc
     6241 cgcgccgcac cgtaaccgcc gcgcaagccc atcagcactt taggcgtggc gacgcggccg
     6301 gaagcgacct cttggaaatc ggcggcacgt tgtgcgtccg taccggcaaa ccgttggaaa
     6361 cggcggctgc ccgcctgttg gttggttacg gtaaaaccgg cattgtaaag gcgggttaag
     6421 gctgtgttga cgcggttggt gtcttcggca aatcctgacg aagcgacaat acgcagaaga
     6481 ttgtcggcag agttggaatg gcgtggtggt ttggggtaca cggttcgggc tttcgcaaca
     6541 ggatgagagg gcgcggatgg ggcattgttt gaagcaggcg cagtaccgca agcctgaagc
     6601 agccccgcgc cggcggcggc ggtgcaggtt ttcaggaaac ggcggcggga ggtcggttcg
     6661 gtcataattt ccctttcata cggtttcaga cggcatcggc gggcttaaag cagcttttta
     6721 agctgttccg tccaatcttg cggcagggtc ataccgtgtt tccggacggg gtttgcccaa
     6781 atcggcgcgg ggaaagacgc atcgttttca aagcgggcga taatatgcca atgcaggtgc
     6841 ggcacgacat tgcccaagct ggcgaggttg atttttgccg gccggaacac ttggcgcata
     6901 gcggcttcga ctttgtacac catttccatc aattcgccgc gttccgctgc cgaaaggtcg
     6961 gtcatttcgg cgatgtgttc atgccagata acacggcaga atgcaggcga accgctgtcg
     7021 ttatggacgg cgatgacgcg cagcttgggg gtttgcagca aaacgtcttc attttgcgcc
     7081 gtgcagatgg ggcagggttg ggcggtcatt tttccttatt cctgaaagtg tttaaaaaac
     7141 ggtaggataa tttctg
//
LOCUS       DAXIEB010000097         7126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000097 DAXIEB010000000
VERSION     DAXIEB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.097"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..354
                     /locus_tag="ACFOA3_001648"
     CDS             <1..354
                     /locus_tag="ACFOA3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898628.1"
                     /translation="HLLPLLEGIAEGTTVYADKGYDSKENRQHLKEHQLLDGIMRKAC
                     RNRPLSETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCL
                     NLLKAANRLSAPAAA"
     gene            472..846
                     /locus_tag="ACFOA3_001649"
     CDS             472..846
                     /locus_tag="ACFOA3_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898629.1"
                     /translation="MSAVGRERNFAKVSYRELVERQLAVRHADLELGLSRAREQEPFV
                     IHVSDLLDKAGIEYAVRMDKDFQTTFNLEYPNTNYDTFKRVVRQMISAYYCVCNDGDE
                     LEISSNRPDGYAVRIVFGDVPV"
     gene            858..1124
                     /locus_tag="ACFOA3_001650"
     CDS             858..1124
                     /locus_tag="ACFOA3_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898630.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQADRKNIAASLERIENKLEKVNEKLDRKADKS"
     gene            1121..1318
                     /locus_tag="ACFOA3_001651"
     CDS             1121..1318
                     /locus_tag="ACFOA3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898631.1"
                     /translation="MKDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTALTF
                     GALGGGVITVGWELLKAKMGL"
     gene            1321..1827
                     /locus_tag="ACFOA3_001652"
     CDS             1321..1827
                     /locus_tag="ACFOA3_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6898632.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQATARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(1824..2336)
                     /locus_tag="ACFOA3_001653"
     CDS             complement(1824..2336)
                     /locus_tag="ACFOA3_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898633.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELALGSFVYRLKAEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLTDGY"
     gene            2348..3970
                     /gene="terL"
                     /locus_tag="ACFOA3_001654"
     CDS             2348..3970
                     /gene="terL"
                     /locus_tag="ACFOA3_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6898634.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAITARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIVIGGYQRSMG
                     RLYVVEAQIKKRLPDLIIEDVICLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            3970..5538
                     /locus_tag="ACFOA3_001655"
     CDS             3970..5538
                     /locus_tag="ACFOA3_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6898635.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5525..6508
                     /locus_tag="ACFOA3_001656"
     CDS             5525..6508
                     /locus_tag="ACFOA3_001656"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFC6898636.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLETFAKFPKIP"
     gene            6539..>7126
                     /locus_tag="ACFOA3_001657"
     CDS             6539..>7126
                     /locus_tag="ACFOA3_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898637.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWHPIEHYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINCQLTEKGLKVEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHT"
BASE COUNT         1872 a         1796 c         1915 g         1543 t
ORIGIN      
        1 cacctgttgc ctttgctgga aggtatagcc gaaggcacga ccgtctatgc cgacaaaggc
       61 tacgacagta aggaaaaccg gcagcatctg aaagaacatc agttactgga cggcattatg
      121 cgcaaagcct gccgcaaccg tccgctgtcg gaaacgcaaa ccaaacgcaa ccggtatttg
      181 tcgaagaccc gttatgtggt tgaacagagc ttcggtacgc tgcaccgtaa attccgctat
      241 gcgcgggcag cctatttcgg gctgctcaaa gtgagtgcgc aaagccacct gaaggcgatg
      301 tgtttgaacc tgttgaaagc cgccaacagg ctaagtgcgc ccgctgccgc ctaaaaggcg
      361 gtccggatgc ctgattatcg ggtatccggg caggattaag ggggtatttg ggtaaaatta
      421 ggcggtattt ggggcgaaaa cagctgaaaa cagccgaaaa cctgtgtttg gatttcggct
      481 gtcgggaggg aaaggaattt tgcaaaggtc tcctaccgtg aattagttga acgtcagttg
      541 gctgtgcgcc atgccgattt ggaattgggc ttaagccgcg cacgcgaaca agagccgttt
      601 gtcattcatg tttccgacct gttggataag gcaggcattg agtacgcggt acgcatggat
      661 aaggattttc agacgacctt taaccttgaa tatccaaata caaactacga cacctttaag
      721 cgtgtagtta ggcagatgat ttcggcgtat tactgcgttt gtaatgatgg agatgaactc
      781 gaaatttcca gcaatcgccc tgacggttac gccgtccgta tcgtattcgg cgatgtgccg
      841 gtttaaaggg gttttaaatg gactttgaat ttggtttcag aaccctgtgg ccgattgcga
      901 cggcggcatt ttggttttgg gtcaacggca tttcaggccg tctgaaagag gcggacaagc
      961 gtatcgatga ccttaaagag gagctgcacg cggtcaagct ctcgtaccac accaagcagg
     1021 atgcccaagc cgaccgcaaa aatatcgcag cgtctttgga gcgcatcgaa aacaagttgg
     1081 aaaaagtaaa cgaaaaactg gacaggaaag cggacaaatc atgaaagacc cgattttgga
     1141 agccttggcg cgtattgaaa acaaaactga tcagacgctg aaaaatcaga aggaaatgca
     1201 ggcggaaatt gcacaaatcc gccaagacac gaaacgcacg gcccttacat tcggcgcact
     1261 gggcggcggc gtgattacgg tcggctggga attgcttaaa gcgaaaatgg gactgtaatt
     1321 atggctcacc cgcaagaaat ccgtgaaaag ttacgtcggc tctatgtgag cggcgagcaa
     1381 actttggaaa cggcggcctt gatgtgcgaa atcccgcagg ccactgcgcg tgcgtggaaa
     1441 cgtgcggata aggaaaaagg cgacgactgg gataagatgc gcgccgccta cactttggcc
     1501 ggcggcggta ttgaggattt gagccgcgcg atgttggccg gttttatggt gcagtacaac
     1561 agcacgatga cgatgctgca ggattcgagc accgaagatt tgccgccgtc cgaccgcgcc
     1621 aagctgttgg ccagcctggc cgatgcgttt accaaaaccg tatcagccaa tgcgcgtgtg
     1681 atgccggaaa cgtcaaaact ggcgacggct ttggaattga ttgagttctt gatggcattt
     1741 gtgcaagaaa aacaccccaa acatttgcct gcctttgtcg aggtattgga gccgtttggg
     1801 gcggaagtgg agaagaagtt tggttaatag ccgtctgtca atctcccata acgtgatcct
     1861 cctactgaaa atacttcaac atctgacttt aaacgctctc ccaatattat ggtttggcaa
     1921 cgggaacagg aatatttatg aaaagcacct tcatacccat tgaattgaag aatcgatttg
     1981 acaccctgct cgtaacattg gggacaaata tcatgaattg gctcgctgtt cgccatctcc
     2041 gctttcaatc gatagacgaa agaaccaaga gcaagttcat acaattggta gcgttgcttc
     2101 tctgtagacc aattttttac tgccaccagt tctttttcca actcatctat acggctaatt
     2161 gccgccgtat aagaagattg ggcttcaaat aaatcagttt gaataccagc aatgatttga
     2221 ttaagttcga tggttttttg cgctacctct gcctctgttt tgagctggtt aaaccctttt
     2281 aaaagtgatc ctgcttcttt taggcctata aatgcgttag aaatgaatcc agtcattata
     2341 aatactcatg aaaacaaaag aattcctcaa atcccttgcc gaactggccg ccagcctgcg
     2401 ccaagtcatc gaagcggaag tggacggctt cgatgcgtcg ccaaaggcta ttactgcacg
     2461 ccgtgccaag gtatttgacc cggtaggcgg ttacgaatat ttcgtgaata cctacttccc
     2521 ccattatatc cgctcgcctg aaaaatccga actgcatgcg tttttgttca gccgtctgcc
     2581 ggagattatc cgctccccca aaggggaaaa tgaggcggta ggtgcgccgc gcggcgaagg
     2641 taaatcgacg caggttaccc agttgtttac gctgtggtgt attgtgaccg gccaaaaaca
     2701 ttatgcggtc atcgtgatgg acagtatcga ccaggcgtat ccaatgctcg aagccattaa
     2761 ggcagaactt gagtttaacc cgcgcctgaa aaccgacttt ccggaagtat gcgggcaagg
     2821 ccgtgtatgg caggccggta cgattgtgac ggccaatgac gttaaagttc aagtggccgg
     2881 tagcggtaaa aagctgcgcg gtttgcgtca cggcccttac cgtcctgact taactgtttt
     2941 ggacgatatt gagaatgacg aacaagtccg caaccccgaa cagcgcgaca agctcaatgc
     3001 gtggctgact aagactgtat tgcctttggg cggtgtcggt cagaaatacg atgtgattta
     3061 tatcggcacg attttgcatt acgacagcgt acttaaccgc actttgaata acccgttttg
     3121 gcggggtgtt aagtttaagg cgatgaaacg ctggcccgac cgcatggact tgtgggacag
     3181 atgggaggaa cttttccgaa acgacggcga gacggtggcc gaggcgtttt atcaggcaaa
     3241 caaagacgag atggagcgcg gcgcagtcac ttcttgggcg gcgcgtggcg tactcgcgct
     3301 gatgaaaatc cgcgcccgtg acggtcatgc gacatttgac agcgaatatc aaaacgaccc
     3361 agtcagtggc gaagatgcgc cgtttgccaa gtcgatgaag ttttggaacg acctgccgtc
     3421 cgatttggtg tatttcggtg cgctcgaccc gtcgctgggt aaagcggggg cgagccgtga
     3481 cccgtcggca atcgtcatcg gcggctatca gcggtcaatg ggcaggcttt atgtcgtgga
     3541 ggcgcaaatc aaaaaacgtc tgcccgacct gattatcgaa gacgttatct gcctgcaccg
     3601 tcaatatcgt tgcaaactgt ggtttgttga gacggtgcag tttcaggagt ttttaaaaga
     3661 cgagctggtc aagcgcagcg cggcgcgtgg aatacctgtc ccggcgcggg cggtcaagcc
     3721 ggtatcggac aagctcttgc ggattgagac tttgcagcct cacatggcga acggtttgat
     3781 tctgttgaat gagagccaac aaacgctgat acagcagttc cgccattttc caaaggctga
     3841 tcatgatgat ggtcctgatg ccgtgcatat gctctggtcg ggggcggtgg cgaattgtgt
     3901 gccgatagaa tggcaaagcc ctaccgataa cgattttgat gacgagataa aaagtaaatg
     3961 gagccgataa tggcaaaaaa ggacaataaa actaaaatcc aaaagcccga ggctgcattg
     4021 cagacggatg cggctcaaat tacggcgacc ggtcgggtta tcgccgagca tccgtccaat
     4081 tttattacgc cgcaaaagat gcgggccctc ttcgaggacg cagaaagcgg cgacatccgc
     4141 gcccaacacg agcttttcgc ggacattgag gagcgcgaca gcgacatcgc ggcaaatatg
     4201 ggtacgcgca aacgcgcgct gctgacgctc aactggcgcg tcgccccgcc gcgaaatgcg
     4261 acgcccgaag aagaaaagct gtccgaccaa gcctacgaaa tgatggacag cctgcctacc
     4321 ctcgaagacc tgattatgga tttgatggac gcggtagggc acggattttc tgcgttggag
     4381 gtcgagtggg tattttcaga cggcctttac ctaccccgaa actttatcca ccgcccgcaa
     4441 agctggttca aatgggacaa agacaacggg ctgctgctgc gtacccgcga aaatccggaa
     4501 ggcgaagcgt tgtggccgct gggctgggtc gttcataccc aaaaatcgcg cagcgtccag
     4561 caggcgcgca acgggctttt ccgcacgctt tcctggctgt atatgttcaa acactacgcc
     4621 gtccacgatt ttgccgagtt tttggagctg tacggcatgc ccatccgtat cggcaaatac
     4681 ggcgcgggcg caaccaaaga ggaaaaaaac accctgcttc gagcggtggc ggaaatcggt
     4741 cacaacgcgg caggcatcat gccagaaggt atggaaatcg agctgcacaa cgctgcaagc
     4801 ggtacgatgg caaccagcaa tccgtttttg cagatggccg actggtgcga aaaatcggcg
     4861 gcgcggctga ttttggggca aacgctaacc agcggtgcgg acggaaaatc cagcaccaac
     4921 gcgctgggca atatccacaa cgaggtacgc cgcgatttgc tggtgtcgga cgcaaaacag
     4981 gtggcgcaaa ccatcacaag ccaaatcatc ggaccgttcc tgcaaatcaa ctatcccaat
     5041 gccgacccaa accgcgtgcc gaaatttgaa tttgacacgc gcgagccgaa agacatcgcg
     5101 gtctttgccg acgctatccc gaaactggtg gatgtcggcg tacaaatccc cgaaagctgg
     5161 gtgcgcgaca aactggtcat tccagatgtg caggagggtg aggctgtgtt ggtgcggcag
     5221 gtaccggaca atccgataaa cagaactgca ttggcggctt tatccgccca caccgtacca
     5281 tctaaggcta cgggcaggca tcaggaaata ttggacggcg cgttggatga cgcgctggtt
     5341 gagcccgatt tcaattctca gctcaacccg atggtgcgtc aggcggttgc cgcacttaat
     5401 gcttgcaaca gctacgagga ggcagatgcc gcactgaatg cgctttatcc gaatttggac
     5461 aacgcgaaac tgcgtaccta tatgcagcag gccttgttta tcagcgatat tttgggacaa
     5521 gaccatgccc gcgcctgatt tgggatttgc cttaagtctg ccgccaaaaa aggcaatcga
     5581 gtggctggaa agtaaaaagg ttacggcgga gagctaccgc aatctgacag cctccgaaat
     5641 tgccaaagtc tatacgattg cccgcatgac cgacttggat atgctcaacg acatcaaaac
     5701 ttcgatggtt gaatcggcaa aaagtggaca gtcgtttgac gattggcgaa aaggtatctt
     5761 gaatctgctc agcaacaagg gctggctgca tccgaacggg cataacggta aggatatcat
     5821 cgacccagcc accggcgagg tattcggttc gccgcggagg ttggagacga tttaccgtac
     5881 caacatgcaa actgcctaca acgccggtca atatcaagga tatatggcaa atattgatgc
     5941 acgaccttat tggatgtatg acgcggtagg cgacagccgc acccgtccgg cgcattcggc
     6001 aatagacggg ctggtgtacc gctacgacga cccgttttgg gcaacgtttt acccgcccaa
     6061 cggctacaac tgccgctgct cggtcatcgc tctgtcggag cgggatgtgg aacgccaggg
     6121 gcggattgtc gggcaaagca cggcggacaa tctggtcgag acccataaaa tctacaacaa
     6181 aaaaggcgat acttatctga cccttgccta taaagcaccg gatggcagtc tgtacacgac
     6241 cgatcgagga tttgattaca acgccggacg aatgaactac cgccccgatt tagacaagta
     6301 cgaccgtgcg ttggcgcatc aatttgccaa agcggaaatg ggtggtgcgg attttaaaac
     6361 cagctttaaa cagcttgaaa aagagtttta tgaagtcaag caacgtttga atattgatgg
     6421 caagcccgat aaagagcaga aaatcaaaat ccgaaacgcg ctatcaagac agcttgagac
     6481 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccatg acatttagga gatttctcat
     6541 gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg accgcttccc
     6601 actattgaag ttggaccagg tgattgattg gcacccgatc gaacactacc tgaaccgtca
     6661 aagaacccgt taccttagag accaccgcgg ccgtcccgcc tacccgctgt tgtccatgtt
     6721 caaagccgtc ctgctcggac aatggcacag cctctccgat cccgaactcg aacacagcct
     6781 catcacccgc atcgacttca acctgttttg ccgttttgac gaactgagca tccccgatta
     6841 cagcacctta tgccgctacc gcaactggct ggcgcaagac gacaccctgt ccgaactgtt
     6901 ggaactgatt aactgccaac tgaccgaaaa aggcttaaaa gtagagaaag catccgccgc
     6961 cgtcattgac gccaccatta ttcagaccgc cggcagcaaa cagcgtcagg ctatagaagt
     7021 cgatgaggaa ggacaaatca gcggtcaaac cacaccgagt aaggacagcg atgcccgttg
     7081 gataaagaaa aacggcctct acaaactcgg ttacaaacaa catacc
//
LOCUS       DAXIEB010000098         7114 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000098 DAXIEB010000000
VERSION     DAXIEB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.098"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(49..987)
                     /locus_tag="ACFOA3_001658"
     CDS             complement(49..987)
                     /locus_tag="ACFOA3_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898638.1"
                     /translation="MDAYPETAANPQSIVELVPVLIAITNGSLRVLTVAKGELLPNGL
                     LAPLRHSLQAGVRMWVAKQTSQPMGYVEQLYTFVDTSRQNEQGLPVLYVSYLGLVREA
                     ADNILHPDAKWQDCYCYFPWEDLRSAGRQHETIVSRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TSVSGSKGRPAQLYRFRDDVLPDQLISDIGLPLGSR"
     gene            1143..2255
                     /gene="nadA"
                     /locus_tag="ACFOA3_001659"
     CDS             1143..2255
                     /gene="nadA"
                     /locus_tag="ACFOA3_001659"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6898639.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            2414..3922
                     /gene="nadB"
                     /locus_tag="ACFOA3_001660"
     CDS             2414..3922
                     /gene="nadB"
                     /locus_tag="ACFOA3_001660"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6898640.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIVQIRRRPNIRVYERQMALDIQTES
                     GAAYGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRADCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(4098..4763)
                     /gene="pgmB"
                     /locus_tag="ACFOA3_001661"
     CDS             complement(4098..4763)
                     /gene="pgmB"
                     /locus_tag="ACFOA3_001661"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6898641.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(4775..7033)
                     /locus_tag="ACFOA3_001662"
     CDS             complement(4775..7033)
                     /locus_tag="ACFOA3_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6898642.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNMDEKRRNFDFYEPMTVHESSLSPC
                     IHAILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLELQVYGKDI
                     TLNGSHTVTLEK"
BASE COUNT         1522 a         1963 c         2039 g         1590 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aggtcgtctg aaaacgggct aacggctgcc
       61 cagtggcagt ccgatatcag aaatcagctg gtcgggcaga acgtcgtcgc ggaagcggta
      121 aagctgcgca ggacggcctt tactgcccga tacgctggta tccgacggct cgatgaggtt
      181 ttgctgctga atctggcggc ggaagttttg tttgtgcagc aatctgccgc tgatggcttc
      241 gacgctgttt tgcagttgca gcagcgtgaa ttcgggcggc atcaattcaa aaatcacggg
      301 gcggtatttg attttggcgc gcaggcggga cagggcggtt gccagcacgc ggcggtggtc
      361 gtggcgcata ggctgccccg tgagcgcgaa gtcgaagttt gcctgcggct cggcggcttc
      421 cgctatcaga ccgctttcat acagcatttc atagcgttgc aaaacgtatt cttccgacca
      481 gttttcaggt tctacccccc agcacaaatg aatgcgcttg agccgctttt ggcgtacttc
      541 ctccgtatcc gcagagtttg cccaaatgcg caggcggctg acgatggtct catgttgcct
      601 accggcgcta cgtaaatctt cccacggaaa atagcagtag cagtcctgcc atttcgcatc
      661 gggatgcagg atgttgtcgg ccgcctcgcg taccagtcct aaatagctga cgtataagac
      721 aggcaggcct tgttcatttt ggcggctcgt atctacaaac gtgtaaagct gttctacata
      781 gcccataggc tgtgatgtct gtttggcaac ccacatcctg acccctgctt gcagagagtg
      841 gcgaagcggt gcaagcaggc cattgggtaa tagctctcct ttggcaactg tcaaaaccct
      901 caagctgccg tttgtaatgg caatcagtac aggaacaagc tcaacgatac tttgcggatt
      961 ggctgctgtt tcaggatagg catccatgcg tggctttcat tcaagagaaa accttattgt
     1021 aaaccaaagc ggttttcaga tgaccttgca tattatactc aatttgagca taatataaaa
     1081 ctatttcaaa atataaacgg caaaccttca tgccgtctga aaaacagcta taaggaaaca
     1141 tcatgcaaac cgccgcccgc cgttcgttcg attacgatat gcccctcatc cagacgccga
     1201 cttccgcctg ccaaatccgt caggcgtggg cgaaggttgc cgacacgccc gaccgagaaa
     1261 cggcaggtcg tctgaaagac gaaatcaagg ctttgctaaa ggagaaaaac gcggtcttgg
     1321 tggcgcatta ttacgtcgat ccgctgattc aggatttggc tttggaaacg ggcggatgtg
     1381 tgggcgattc gctggaaatg gcacgcttcg gtgcggaaca tgaagccggt acattggtgg
     1441 tagcgggtgt gcgcttcatg ggcgagagcg cgaaaatcct ctgccctgaa aaaacggtgc
     1501 tgatgcctga tttggaagcg gaatgttctt tggatttggg ttgcccggaa gaggcgtttt
     1561 cggcgttttg cgaccaacac cccgaccgca cggtggtggt gtacgccaac acttccgccg
     1621 ccgtgaaagc gcgtgccgac tgggtggtaa cgtcttcagt ggcgttggaa atcgtgtcgt
     1681 atctgaaatc gcgcggcgag aagctgattt ggggacctga ccgccacctc ggcgactaca
     1741 tccgccgcga aacgggcgcg gatatgctgt tgtggcaggg ttcgtgcatc gttcacaacg
     1801 aattcaaagg gcaggaactg gcggcgttga aggcggaaca ccccgacgcg gtggtgctgg
     1861 tacatccaga atcgccgcaa agcgtcatcg aactgggcga cgtggtcggc tcgaccagca
     1921 aactgctgaa agccgccgta tcgcgccctg aaaaaaaatt catcgtggcg accgatttgg
     1981 gcatcctgca cgaaatgcaa aagcaggcac ccgacaaaga gtttatcgcc gcaccgacgg
     2041 cgggcaacgg cggaagctgc aaaagctgcg cgttctgccc gtggatggcg atgaattcgc
     2101 tgggcggcat caaacacgcc ctgacaagcg gacacaacga aatcctgttg gacaggaagc
     2161 tgggcgaagc cgccaaacta cctttgcagc gtatgctcga cttcgcggca ggactcaaac
     2221 gcggagatgt gttcaacggc atgggccctg cctgatttgc cgccaatatt atagtggatt
     2281 aaatttaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga
     2341 ggcaacgccg tactggttta aatttaatcc actataccgt ttcagaccgc ctctcaaaca
     2401 aggacaacac catatgcaaa ccgattgcga cgtattgatt gccggaaacg ggctggcggc
     2461 actgacgctc gccctgtcgc tgcctgaatc gttccgcatc gtcattttgt gtaaaaaccg
     2521 gctggacgac accgccagcc gtcatgcgca aggcgggatt gcggcggcgt ggtcgggaga
     2581 ggacgacatc gaaaaacacg tcgccgatac tttggaagcg ggcgcgggtt tgtgcgacga
     2641 agccgccgtc cgcaccatcc tgtcgcaggg caaaccggca atcgaatggc tgctggcgca
     2701 gggtgtggcg ttcgaccaaa ataataacga cctgcacctg acacgcgaag gcgggcacac
     2761 ctgccgccga atcgcccacg tcgccgacta cacgggcgaa gccgtcatgc agagcctgat
     2821 tgtccaaata cgccgtcgcc cgaacatccg cgtttacgag cggcagatgg cgttggacat
     2881 ccaaaccgaa tcaggcgcag cgtacggact gaccgtcctc gactgccgaa cgcaagaaac
     2941 ctaccgcatc cgcgcccgcc ataccgtact cgcaggcggt ggcttgggac agatttacgc
     3001 cgccaccacc acgccgcccg aatgcacggg cgacgccatc gccatggcga tacgcgcaga
     3061 ctgcgcagtc gaaaatctcg aatttatcca attccacccc acaggcttgg caaggccgtc
     3121 tgaaaacggc cgcaccttcc tgatttccga agccgtgcgc ggcgaaggcg gcatcctgac
     3181 caaccaagcg ggcgaacggt ttatgccgca ttacgaccgc cgcgccgaac tcgcgccgcg
     3241 cgacatcgtt gcccgcgcca tcgccgccga aatcgccaaa caaactccag acttcgtctc
     3301 gctcgacatc agccgccaac ccgaagcgtt cgtccgccgg cattttccgt ccatccatcg
     3361 gcactgttta tcccaatgcg gcctggacat cacgcgccaa gccatccccg tccgccccgt
     3421 gcaacactac acctgcggcg gcatccaaac cgacccctgc ggcagaacct ccctgccgca
     3481 gctctacgcc ttaggcgaaa ccgcctgcac cggcttgcac ggagccaacc gcctcgccag
     3541 caactccctg ctcgaatgtg tcgttaccgc cagacttgcc gcccaaacca tcgcggacgg
     3601 acaagcattc caagccaaaa cgtctccaag gccgtctgaa aacccctccg ccgaagcagg
     3661 catctttgca gacgaccttc aaaacacatt cagccgcccc gtcctgcaaa cgttcaacca
     3721 acgccatctc ggcatcctac gcagcgatac cggcctgcgc cgcgccatcg cccaactgcg
     3781 gctttggaag caaaaccaag ccgaaccgca caccgcgtcc gaatacgaaa accgcaacct
     3841 gctcgaatgc agcctcgccg tcgcccaagc cgcatacagg cggagacaga acatcggcgc
     3901 gcattttaat agtgattgtt aaggttggct ggttagaaaa aatcgttcaa atgaatttta
     3961 atagcatggt taagagattg gcattcagtt tttagggttg cgtaagttgg gttatcaatc
     4021 tgttctaaaa gcagaaaggc cgtctgaaac actgttcaga cggcctttta cttccttgac
     4081 actttatctg acgcgtttta cctgcccgac tgtgcccaca cgttttgcag gtaggcgtag
     4141 gtcagctcgg cggtatcgga caccagcgcg atgtcgctgc ccaagtcttc ggctttgccc
     4201 acgccgatag gcaaggcgcc ggcggctttg atggcggcga cacctgcggc agcgtcttca
     4261 atgccgatgc agcggcggat gtccgcgccc acgccctcgg cggcggcgag gaagatgtcg
     4321 ggggcgggtt tggaatgcgc gacggcggca gggtcggcga cggtgtcgaa gaagtgggtc
     4381 agccccatgc gttcgagcag gaaggggccg tttttactgg cggacgcaag ggcgattttt
     4441 ttgccgtttg cttttaatgc ttccagcagc ggcaaaatgc cgagatacac gtcttcgggt
     4501 ttgactgcct gaatcatctc gacgtagttg tcgtttttgc ggcgggtcag ttcggcgaac
     4561 tcggcttcgc cgacggtttt gccgccgtgt gcgaggatgc gtttgagcga atcgtcgcgc
     4621 gacacgcctt tgagctgctc gttaaacttg cggtctatgc cgatgcccag ttcttcggcg
     4681 agctttttcc atgcgcggta gtggtattcg gcggtgtcgg tgatgacgcc gtcgagatcg
     4741 aagagcactg cggtaaacgt cattttgcgc ccccttattt ttccaacgtg acggtatggc
     4801 tgccgttgag cgtgatgtct ttgccgtaca cctgcaattc gagcggttcg cctttgagca
     4861 gggtgaagac gacgttttct ttgccgacgg cgactttaat cagacggcct cggtagttga
     4921 tgtggaaggc gtagcctgtc cacgcactcg gcaggaacgg tgcgaagctg agtgtgccgc
     4981 cccaagtttt catttgggcg aaaccttgga cgatggcgag ccacgagccg gtcatggagg
     5041 taatgtgcag gccgtcttcg gtgtcgttat tgtagttgtc caagtccaga cgggcggtgc
     5101 gctggtacat ttccacggct ttttcttctt tgcccagttc ggcggcaagg atggcatgaa
     5161 tacacggcga caacgagctt tcatgcacgg tcatcggttc gtagaagtcg aagttgcgac
     5221 gtttttcgtc catattgaaa cggtcgccga agaagtagat gccttgcaag acgtccgcct
     5281 gtttgatgaa gggcgaacgc aggattttgt cccacgacca tttctggttg agcggcaaat
     5341 cgtcgggcga aagcgcggac actgggcgga tgtctttatc aaggaagccg tcgtgttgga
     5401 cgaatacgcc gagttcttcg tcatgaggac ggtacatatt cgcgctgatg tccgcccatt
     5461 tttccaactc ggcggcacgc acgttcaaat ccggacgcgg gtatttcgcc aaggcttcgc
     5521 gggtgtagtc caatacccaa gcggcaaggg tgttggtgta ccagttgttg ttgatgttgt
     5581 tttcgtattc gttcgggccg gttacgccgt gaatcatgta tttacccctg cgtttggaga
     5641 agtggacgcg gtccgcccag aagcgggaca cttcgaccaa gacttccaag ccttctttgg
     5701 caagatagct ttcgtcgccg gtgtagttgg tgtagttgta gatggcgtaa ggaatcgcgc
     5761 cgttgcggtg gatttcctcg aaggtgattt cccattcgtt gtggcactcg atgcccgtaa
     5821 acgttaccat cggatagagt gcgcccgcca agccctgttc gcgcgcgttg tgctgcgcct
     5881 gcggcagttg gttgcggcgg tattgcagca ggttgcgggt aacttcgggt tccgccagtg
     5941 cgaggtaaag cggcacggcg taggcttcgg tgtcccaata ggtcgcgccg ccgtattttt
     6001 cgccggtaaa gcctttcggg ccgatgttca ggcgcgcgtc ttcgccgtag taggtggaga
     6061 acagttggaa caggttgaag cggatgccct gctgcgcttc gtcgctgcct tcgatgacca
     6121 cgtcggcgat ttcccaacgg tgcagccagc ctgctttgtg cgcgtccagc aaggtttcaa
     6181 acgcaacgcc tgcgattttt tccgacaagg cgcggcctgc ggctttcact gcttccaagc
     6241 cctgataatc gcggctggtg gtaacaatca cgcgtttttc aaaggtttcg ggcgtgccac
     6301 cgacttcggc ttcaaaagaa ttggagacct gccagtcggt ttgtctgccg ccgagggctt
     6361 taaagctgcc ggcgaaggtt tgctcggcgt tgacgataaa ttgttccacg ccgaaagggt
     6421 tggcaacggt ttgggcggca atgtaggaga gactgtctga aacgcctttg tccaatacct
     6481 gccagaattt ttcttcgtag ttggagtctt cgtttttcac gtcggcatcg atgatggaat
     6541 cgatgcggac ttggtgggtt ttgccgtcaa cggatacggc ttcccagcgg atgagagcca
     6601 gctctttttg tgcgacggac aggaatttgc acacatcgaa acgcacgccg aatacggtga
     6661 acgagcggcg caacacgccg tgctgcatat cgagttcgac ggagaagccg gcaacatcgt
     6721 ttttcgccaa gtccacctct tgaccatcga caaagatttt gactttgctg aaattgagcg
     6781 cgttgatggc tttgccgaaa tatttggggt agccgttttt ccaccagccg acgcgggttt
     6841 tgtcgggaaa ccacacaccg gcgatgtagg tacccaagtg gctgtcggcg gaataggttt
     6901 cctcaaagct gccgcgcata cccatatagc cgttgcccaa actggtcagg ctctcttgca
     6961 gccgtttgtg ttctttttcc agctttgccg aacgcagcgt ccaagggctg atttccataa
     7021 ttcttgtgta catttatgaa gctcctgttt ggattgattt gagggaatgg tgaaatctta
     7081 tagtggatta acaaaaatca ggacaaggcg acga
//
LOCUS       DAXIEB010000099         7087 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000099 DAXIEB010000000
VERSION     DAXIEB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.099"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..810
                     /locus_tag="ACFOA3_001663"
     CDS             <1..810
                     /locus_tag="ACFOA3_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6898643.1"
                     /translation="GQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNW
                     LAQDDTLSELLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQAIEVDEEGQVS
                     SQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLPPLLEG
                     LPKGTTVYVDKGYDSAENRQHLEEHQLQDGIMRKACRNRPLTEVQTKRNRYLSKTHYV
                     VEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(1061..1273)
                     /locus_tag="ACFOA3_001664"
     CDS             complement(1061..1273)
                     /locus_tag="ACFOA3_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6898644.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(1285..1566)
                     /locus_tag="ACFOA3_001665"
     CDS             complement(1285..1566)
                     /locus_tag="ACFOA3_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898645.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGRHYRHSENGITLYDFIFFDGKPADEARFAAILTETCRRAVKKICETK"
     gene            complement(1604..1807)
                     /locus_tag="ACFOA3_001666"
     CDS             complement(1604..1807)
                     /locus_tag="ACFOA3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898646.1"
                     /translation="MNIIEQETKRAELRKIDAEIAKIIADAHKINAESVKIAQESRWY
                     PMIAATGLVTAIAAVLALIFKFA"
     gene            complement(1887..2459)
                     /locus_tag="ACFOA3_001667"
     CDS             complement(1887..2459)
                     /locus_tag="ACFOA3_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6898647.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(2434..2778)
                     /locus_tag="ACFOA3_001668"
                     /pseudo
     CDS             complement(2434..2778)
                     /locus_tag="ACFOA3_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2771..3646)
                     /locus_tag="ACFOA3_001669"
     CDS             complement(2771..3646)
                     /locus_tag="ACFOA3_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6898648.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3691..3981)
                     /locus_tag="ACFOA3_001670"
     CDS             complement(3691..3981)
                     /locus_tag="ACFOA3_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6898649.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3993..4196)
                     /locus_tag="ACFOA3_001671"
     CDS             complement(3993..4196)
                     /locus_tag="ACFOA3_001671"
                     /inference="COORDINATES: protein motif:HMM:TIGR04111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6898650.1"
                     /translation="MLSQWERAIYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYL
                     VVGGQRKGNYGKGHEIAKKLGLK"
     gene            4286..4663
                     /locus_tag="ACFOA3_001672"
     CDS             4286..4663
                     /locus_tag="ACFOA3_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6898651.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            4692..4937
                     /locus_tag="ACFOA3_001673"
     CDS             4692..4937
                     /locus_tag="ACFOA3_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898652.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            5171..5320
                     /locus_tag="ACFOA3_001674"
     CDS             5171..5320
                     /locus_tag="ACFOA3_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898653.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLT"
     gene            5336..5842
                     /locus_tag="ACFOA3_001675"
     CDS             5336..5842
                     /locus_tag="ACFOA3_001675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898654.1"
                     /translation="MDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQ
                     APPSGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNI
                     EFKAVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSE
                     LSDEELEA"
     gene            5915..6139
                     /locus_tag="ACFOA3_001676"
     CDS             5915..6139
                     /locus_tag="ACFOA3_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898655.1"
                     /translation="MQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRY
                     DEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            6136..6618
                     /locus_tag="ACFOA3_001677"
     CDS             6136..6618
                     /locus_tag="ACFOA3_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898656.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            6708..6878
                     /locus_tag="ACFOA3_001678"
                     /pseudo
     CDS             6708..6878
                     /locus_tag="ACFOA3_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
BASE COUNT         1748 a         1681 c         1739 g         1919 t
ORIGIN      
        1 ggacaatggc acagtctctc cgatcccgaa ctcgaacaca gcctcattac ccgcatcgat
       61 ttcaacctgt tttgccgttt tgacgaactg agcatccccg attacagcac cttatgccgc
      121 taccgcaact ggctggcgca agacgacacc ctgtccgaat tgctcaaact gattaaccga
      181 caactgaccg aaaaaggctt aaaagtagag aaagcatccg ccgccgtcgt tgacgccacc
      241 attattcaga ccgccggcag caaacagcgt caggccatag aagtcgatga agaaggacaa
      301 gtcagcagcc aaaccacacc gagtaaggac agcgatgccc gttggatcaa gaaaaacggc
      361 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cagaaggcta tatcgagaaa
      421 ctgcacatta cccccgccaa tgcccatgag tgcaaacacc tgccgccgtt gttggaagga
      481 ctgcccaaag gtacgaccgt ctatgtcgac aaaggctacg acagtgcgga aaaccggcaa
      541 catctggaag aacatcagtt gcaggacggc attatgcgca aagcctgccg caaccgtccg
      601 ctgacggaag tgcaaaccaa acgcaaccga tatttatcga agacccatta tgtggtcgaa
      661 caaagcttcg gtacgctgca ccgtaaattc cgctacgctc gggcagccta tttcggactg
      721 attaaagtga gtgcgcaaag ccacctgaag gcgatgtgtt tgaacctttt gaaagcggcc
      781 aacaggctaa gtgcgcccgc tgccgcctaa aaggcggccc ggatgcctga ttatcgggta
      841 tccggggagg attaaggggg tatttgggta gaattaggag gtatttgggg cgaaaacagc
      901 tgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcag
      961 agtgagttta ttttggggcg gcggcaggtc tggggcaagc ggtgtggggc ttggttgtgg
     1021 tttttaggtt tttgggggta aaaaatgccg tctgaacttt tcagacggcg tttgtttttt
     1081 ctatccaatc gaggaactgc cgccattttt ccagcggcat atcggcccga cgggtttcgg
     1141 tatcgggttc ggcttcccag cggcctacct gtatgtagtg cttcaccccg acgatttgcg
     1201 ccaactcggc ctgtgtcagt ttgcagcggt tgcgaagggt gcggaggttg taaggcgtgt
     1261 agccgagttc catgtcgttg cggtttattt tgtttcgcat atttttttga ctgcccggcg
     1321 gcaggtttcg gtaaggatgg cggcaaatcg ggcttcgtct gccggtttgc cgtcgaaaaa
     1381 aataaaatcg taaagggtga tgccgttttc ggaatggcgg taatgcctgc ccgggcattc
     1441 gccgtctgtc tgcctgtgga ttttggcaat caggcgggga tagcggcaat gggtaatgaa
     1501 acggcttgcg ccgtcttgcc cgataatcca ttcggggtag cggttgaata gtgcggctct
     1561 gttcattttg ttcgtgggat aaagcccctc gcggggcttg tggtcaggca aatttgaata
     1621 tcagtgccaa cacggcggcg atggcggtaa ccagcccggt ggccgctatc atgggatacc
     1681 agcgggactc ttgggctatt tttacggatt cggcgttgat tttgtgcgcg tcggcaatga
     1741 ttttggcgat ttcggcatct atttttctta actcggcgcg tttcgtttct tgttcgatta
     1801 tgttcattgg atttcctttc gtttttggcg gttgccgccg cttgtcggat ggcaggatgt
     1861 ctgccatgtg tatatattga taccttttag gttttattgc aagtgttttg ggcggcggct
     1921 tcgtatgctt ggcggtggcg gcggctgtac cattcggcga gttcgcgccg ctcttggagg
     1981 cggtagctgt cggcggtcag gtagtggtgc aggcttgaga agccggcgcg ttggagggcg
     2041 gcgcggctga tgtggccgag ggcgaggtct gttgtgaggg tgtcttttcc ggcggtcagg
     2101 ctgatgttgg tgaagaggtg gcggaatccg tgcattgtgt gtttggattt gccgggggtg
     2161 ctgccgtcat agcccagtcg tcggatggcg ttgtgggcga atttgatgct gatgtggtcg
     2221 ggatgggggg cgggtttgcg ccgtgggcgg atgccgggga aaaggtggat gttgtcgccg
     2281 gtctgtgtgt gcagctctcg gagtatttct accgcccagt ccgacagtgg gacggtaaag
     2341 gggtgtttgg tcttcatgtc ggcggcgggg atgtgccata accgggcggt gaggtcgatg
     2401 tcttgccagc gggcggaaag cagtgcggag ggacgggatg acttccccgg tttccccatc
     2461 aatccgcgcc gcctcgattt ttccgttggc gcgcagcatt tccaccgcct gccgcaccga
     2521 catcaaccgc ttgccgttgc gcctcatcgc ctccaccaaa accgccgaaa tcaatttggc
     2581 ttcttccggt ttaagctccg tcttgcccgc atcgctgcgc cgtttgcgcg tcggcttgac
     2641 gctgaccgcc tccagcttgc ggtataacgt ggcaaggctg atgcccaatt cctgcgcctg
     2701 ctgcttaaga tatgcagagc gtgcgccgcg ttccattgct tccgcctgat tctcgactgc
     2761 cttaagacgc tcaatcattg ccggattcat cgccttctcc cgtttcaccg cccaaccatt
     2821 ccggcacatt gtctgtcggt gcttcagtcg gcagggcata gctttcgcgc agttgctcgc
     2881 agtccaaaat aatttgattg agcgtgccga ccatctttgc ctgatggctg atcccgtgtg
     2941 cctcactgtg cgcattaagt tggtcgaaca aatctttcag acggctcact tgactgcgga
     3001 taccgacctc aaggcttgtt aactgcatcg ccaactcgct gcctacgtct tccgccttcg
     3061 gctctctgac aacggtttgc ttcttagcca gcttctcggc cagctcatca attttggctg
     3121 ttttggtttt catcacttcg tctttggcgg cgaggttttc gcggctttcg cgcagggcga
     3181 cgcgcagctc gcgcaccgtc attcggtcca catcgtcaaa ggtcatgccg ttgacttctt
     3241 ccccttcggc caaacccacc agcgtaacgt cttcttcgac cagcagctca agcagcttcg
     3301 acttgcccaa atccatcagc ttcggcgcgg ctttctgcat ttgcggggtc gcaaagcggc
     3361 gagtggctga catcagacgt gatgtttctg cgatacccag cccaaattgg cttttcacaa
     3421 tttccataaa ccgtccgtgt tccgtatgct cttttaaaat aatcagcgca cgtcccagtt
     3481 cgaacatgcc ttccatcgtc tgccgtactg cttgacgacc gcgttcaacc caacgctctt
     3541 cgctataagt ctcgccgtta ccccactgct ccatcaccag cacactgcgc atcgctgcct
     3601 gattgcttac tttgtcggtt gcgataattt caatttctgt attcattttt tatctccaaa
     3661 gtttccgacg tcggaaactt tcaaaatccg ttaatccaca tcgacccgct tgccgatttc
     3721 ggcaatcttg ctttgcagcc gttcatgctg ctgcctgaac cgctctgcga tttgcagggt
     3781 tttgatgccg taggcgtagt tgccgttttc aagtttgatg accaatcccg aggcaaccaa
     3841 atcatcaata tccctgctga cttgcgatgg cgtcagcccc agtccgaccg ataaatcctt
     3901 attgctcaga ccgataatcg gatgctcgtc aagcgcgata aagaccctca atagccgttg
     3961 taccctttta ctttctgcca tccgcatcct ccttatttca gtcccagctt cttggcaatt
     4021 tcatgcccct tgccgtaatt gcctttgcgc tgtccgccga ccaccagata cacatcgcgc
     4081 ggcttaaagc cgttctctct tgcccactgc gccagcgttt ggccgttttg ggcaaaattt
     4141 tcttttagtt tttcaacagt atagccatag atagcccttt cccattgtga taaaatagtg
     4201 aactatttat gcactagaaa ttaattagtg cattaccaat gcgtgaatta tgagatttta
     4261 aaatctcttt tacaaggatt attttgtgat ttctaaatct ctttttggta atcgtttgaa
     4321 agaaaaaaga aagtttttag ggcttaccca agcacaagct gctgaaaaag caggtattga
     4381 gcgcgaaaca tggggcaaat acgaacgtgg cgtatttatg cctagtggag atgtattgct
     4441 ctcttttttg aatatgggaa tagatgtgag cagcttattt gctgctgaac agggaattag
     4501 gccgtctgaa aattctgaaa ttagtaagga agagtcagag ttattagggt attaccgcca
     4561 agccagcgat aacggcaaat ttgtcattct cagtgtggca aaaggcgcag agaaaaaagc
     4621 agccgaaaat actcaagttt cagccacgca taaaacagcg tgatttttaa atccaaaatg
     4681 gagacagtct tatgtttgac tttgaacaac aaatcaaatg gggtgagcga gcggaagaaa
     4741 tcgttaaaga ggcagccacc cagaacaata tcgaaattcc cgaacctttg gcttcggcat
     4801 tagctaaggc ggtaaaagtg cattatctaa gccaagctgg tgtgttttct ttggtagaag
     4861 cctatgcaga cacggtaaat ccaactgaaa aagaagtaga ttaccaagca atcggcaaag
     4921 agctatttga aaaataaaaa atgccgtctg aaattttcag acggcattta ggacactccg
     4981 acagtcgggc tgctccggtg ttctgaaacg gatattaagc aattgaagcg gccgtgcatt
     5041 ttaaaacgca ttaaaaacta ccccgacata caggtcatcg ggcaggtggt tcaggtgttg
     5101 aaagatttga attgatgccg tctgaattta ttttttcgac acagatatta aaagagaaag
     5161 gactgtacaa atggcaaaaa aaactactaa aactattcat tatttacgtg cgcagcaaaa
     5221 ccatagcttg ttcgatttag aggtagcatt aagaagggtt ttgtcagctg ctcctactgt
     5281 tcaagataca caaattgaac aatacaatca gcttacctaa atcatgcata gaactttgga
     5341 tcctaaagat gtaaaaggca attcaattgg tggattactg atacacattg gcagcggtac
     5401 aaaagatgaa catatccgca ctatgagcaa tagacctgta cagcaagatg atcatggtgg
     5461 aacacaagct cctccaagtg gatattcctt tttgcgtaaa gaggcttttt tgtatattgt
     5521 tggacaccat gtaatttttt gcggacatgg ttttttgtca gcatcaacgg ttgcttctta
     5581 tttaagttta cttagcaata aattgagaga gaacaatccg gatattgttc aatttaatat
     5641 tgaattcaaa gcagtaggca attgcgacaa gttgtcattg atacaacaac atggggtaaa
     5701 aagtattctt ttagatgctt cggcatatca attatccaga aacagactgt atcaagatag
     5761 ccgttcaacc attgctaaag cattagggaa agttggtagt gtatttacaa gcgaattgag
     5821 tgatgaggaa ttggaagcat aatccgaaat tcatatcaat ttagaagtct tgctaaacgg
     5881 taattcgaga gccagtattg aagcgcaatc gctgatgcag gagcaggcag aggaaattat
     5941 tgatgatgaa acggttaatc aaggattttc cattaccacg caacaaggtg aagtgattca
     6001 gccctctgat gtaaaactgt ctaaatctgt tagaatagga cgatacgatg aagccaactc
     6061 gttactccct gactcggcat ttacagcaat cagtgaatat ttcctagaac tccaaagcag
     6121 aaacttaact gaacaatgaa aaaatatctg cccttactac gttttttgtt gattgcttgt
     6181 atatcagcat ttttagcatg gaaagggcaa ccttttgttc atgggaacga gaaggccgtt
     6241 gatttaatta ttaacgtatt tgcaatctta gccggttttt taattgccat tatgacattg
     6301 tttagtgata tgaggtttga tgaagatgca aattggcgac aaattcaaat tcgtgaaggc
     6361 gtacaagaac aacggtatat aaaacattcc ttactttttt acacatatct tgccgtatta
     6421 gtttgtgttt ttattgtcat cttgttggct cataaagaag aatacaaaaa tggaccagcc
     6481 attttttggc ttgaacggag ctatttattt ttagcctgta tttccatttt ttattctgta
     6541 ttcttacctg ggaatttaat taaaagccga aaagaagagt ttaaaaaatt aatggaaaag
     6601 aagaaaccca aaatctagaa cgagttttaa ccccctttaa aatcccattc agacgacctt
     6661 ttctaaaatc cctgtattga tttttaattc aatacaggga tttttccatg tcagacaagt
     6721 tcaaccaatt catcaaccgc gtcctctctc acgagggtgg ttacgccaac catcccaaag
     6781 accccggcgg cgaaaccaat tggggcatca ctaagcgcac cgcacaggca aacggctaca
     6841 acggctccat gcgtgccatg acgcgtgaac aggcaatcag agacctttgc aaaattcctt
     6901 tccctcccga cagccgaaac cccaacacag gttttcggct gttttcgccc caaataccgc
     6961 ctaattctac ccaaatcccc ccttaatcct ccccggatac ccgataatca ggcatccggg
     7021 ccgcctttta ggcggcaaca ggcacactta gcctgttggc cgctttcaac aggttcaaac
     7081 acatcgc
//
LOCUS       DAXIEB010000100         7015 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000100 DAXIEB010000000
VERSION     DAXIEB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.100"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFOA3_001679"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFOA3_001679"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6898657.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1553..1708
                     /locus_tag="ACFOA3_001680"
     CDS             1553..1708
                     /locus_tag="ACFOA3_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898658.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1705..2136
                     /locus_tag="ACFOA3_001681"
     CDS             1705..2136
                     /locus_tag="ACFOA3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6898659.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2270..6598
                     /gene="app"
                     /locus_tag="ACFOA3_001682"
     CDS             2270..6598
                     /gene="app"
                     /locus_tag="ACFOA3_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6898660.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGIFPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNVGYRDVDFGAEGRNPDQHRFTYKIVKRNNYKKDETHPYEKDYHN
                     PRLHKFVTEAAPIDMTSDMDGNKYTDRTKYPERVRIGSGWQFWRNDQDKGDQIAGAYH
                     YLTAGNTHNQGGAGGGWSSLSGDVRQAGNYGPIPIAGSSGDSGSPMFIYDAEKQKWLI
                     NGVLRTGNPWAGKENTFQLVRKSFFDEILEKDLRTSFYSPSGNGAYTITDKGDGNGIV
                     KQQTGRPSEVRIGLKDDKLPAEGKDDVYQYQGPNIYLPRLNNGGNLYFGDQKNGTVTL
                     STNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRLSKIGKG
                     TLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNP
                     DKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNN
                     NSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGK
                     LFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKAENFLIKGGQAVVSRN
                     VAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDEKVIASLTNTD
                     ISGNVSLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQNGNLSLVGNAQATFNQA
                     TLNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAFNGNVSLADKAVFHFENS
                     RLTGQISGGKDTALHLKDSEWMLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSAT
                     DAPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKL
                     AESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQL
                     IRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRDAVEKTESVAE
                     PARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARRDLPQP
                     QPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDT
                     KHYRSQDFRAYSQQTNLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGA
                     VFGQYGIGRFDIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGIEPH
                     IGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTD
                     AASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGI
                     KLGYRW"
BASE COUNT         1843 a         2057 c         1733 g         1382 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg
       61 atgctttcga tccgtttatt ttccgatgca gaatctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccagcc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc tttcgccctg
      301 ccaaccggcc tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacagcgcg atgacggttt ctgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatgcgttcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcaccgg aacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccagcgc
      721 gttcaacagg ctggatttgc cgacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccaatttg ccgcgcgcat ccgctgcttc gagaaaatca atgtcttcct cgggaaaatc
      901 cagcgtcgct tcgaccagca tccgcaaggt aatcaaatct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cctccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc
     1081 acgcttcgta aactcgcccg gctcggcaag gcgcgcgccc agttccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcgcccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgcta tcgattgcct gtccgtccgc
     1261 gtccgtaaaa tcagcatagg ttgcggtacg cggtttaggc gttttcccgc acaaagcttg
     1321 cgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgtccggg
     1381 cgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca
     1501 taaaaccgca aaaatgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa
     1561 acgaaacaca tacgccttcc tgctcggtat aggttcgctg ttgggcctgt tccatcccgc
     1621 aaaaaccgcc atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca
     1681 acgcgccata gagaaagcgc gcaaatgacc gaaaacgcac aggacaaggc gcggcaggct
     1741 gtcgaaaccg tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg
     1801 caaataatga tagctcggcg tttccattcg ggaccgttgc cgccgccgtc cgacttggcg
     1861 caatacaacg acattatcag caacggggca gaccgcatta tggcaatggc ggaaaaagaa
     1921 caagccgtcc ggcacgaaac catacggcaa gaccaaacct tcaacaggcg cgggcaactg
     1981 tacggcttca tcagcgtcat cctgatactg ctttttgccg tcttcctcgt atggagcggc
     2041 taccccgcaa ccgccgcctc ccttgccggc ggcacagtgg ttgccttggc gggtgctttc
     2101 gtgattggaa gaagccgaga ccaaggcaaa aattaatgac aaatcctagg gcgtgcttca
     2161 tatccgcccg aacgccgaac cgcacatata ggcacatccc gcgcgccgcc cggaagcgga
     2221 agccgcgccc tcccaaacaa acccgaatcc cgtcagataa ggaaaaataa tgaaaacaac
     2281 cgacaaacgg acaaccgaaa cacaccgcaa agccccgaaa accggccgca tccgcttctc
     2341 gcctgcttac ttagccatat gcctgtcgtt cggcattttt ccccaagcct gggcgggaca
     2401 cacttatttc ggcatcaact accaatacta tcgcgacttt gccgaaaata aaggcaagtt
     2461 tgcagtcggg gcgaaagata ttgaggttta caacaaaaaa ggggagttgg tcggcaaatc
     2521 aatgacaaaa gccccgatga ttgatttttc tgtggtatcg cgtaacggcg tggcggcatt
     2581 ggtgggcgat caatatattg tgagcgtggc acataatgta ggttatcgcg atgttgattt
     2641 tggtgcagag gggcgaaatc ccgatcaaca tcgttttact tataaaattg tgaaacggaa
     2701 taattataaa aaggatgaaa cgcatcctta tgagaaagac taccacaacc cccgtttgca
     2761 taaatttgtt acggaagccg ccccgatcga tatgacttct gatatggacg gcaacaaata
     2821 tacagatagg acgaaatatc ccgaacgcgt ccgtatcggc tccgggtggc agttttggcg
     2881 aaacgatcaa gacaaaggag accaaattgc cggcgcatat cattacctga cagcaggcaa
     2941 tacacacaat caaggcggag cagggggcgg ctggtcaagc ctgagcggcg atgtgcgcca
     3001 ggcaggcaat tacggtccca ttcctattgc gggctcaagc ggcgacagcg gttcgcccat
     3061 gtttatttat gatgcggaaa aacaaaaatg gttgattaac ggcgttttga ggacaggtaa
     3121 cccttgggcg gggaaagaaa atacattcca actggtacgc aagtcttttt ttgatgaaat
     3181 ccttgaaaaa gatttacgta catcgtttta tagcccatcg ggcaatggtg catacaccat
     3241 tacggacaaa ggcgacggca acggcattgt caaacaacaa acaggaagac catctgaagt
     3301 ccgaatcggc ttgaaagacg acaaattgcc tgccgaaggt aaagacgatg tttaccaata
     3361 ccaaggtcca aatatatacc tgcctcgttt gaataacggt ggaaacctgt atttcggaga
     3421 ccaaaaaaac ggcactgtta ccttgtccac caacatcaac caaggcgcgg gcggtttgta
     3481 ttttgaaggt gactttacgg tctcgcctga aaataacgaa acgtggcaag gcgcgggcgt
     3541 tcatatcagt gaagacagta ccgttacttg gaaagtcaat ggcgtggcaa acgaccgcct
     3601 gtccaaaatc ggcaaaggca cgctgcacgt tcaagccaaa ggggaaaacc aaggctcgat
     3661 cagcgtgggc gacggtaaag ttattttaga tcaacaagca gatgaaaata ataaaaaaca
     3721 agcctttagt gaaatcggct tggtcagcgg caggggtacg gtgcaactga atgccgataa
     3781 tcagttcaac cccgacaaac tctatttcgg ctttcgcggc ggacgtttgg atttgaacgg
     3841 gcattcgctt tcgttccacc gtattcaaaa taccgatgaa ggggcgatga ttgtcaacca
     3901 caatcaagac aaagaatcca ccgttaccat tacaggcaat aaagatattg ctacaaccgg
     3961 caataacaac agcttggata gcaaaaaaga aattgcctac aacggttggt ttggcgagaa
     4021 agatacgacc aaaacgaacg ggcggctcaa ccttgtttac cagcccgccg cagaagaccg
     4081 caccctgctg ctttccggcg gaacaaattt aaacggcaac atcacccaaa caaacggcaa
     4141 actgtttttc agcggcagac cgacaccgca cgcctacaat catttaaacg accattggtc
     4201 gcaaaaagag ggcattcctc gcggggaaat cgtgtgggac aacgactgga tcaaccgcac
     4261 atttaaagcg gaaaatttcc tgattaaagg cggacaggcg gtggtttccc gcaatgttgc
     4321 caaagtagaa ggcgattggc atttgagcaa tcacgcccaa gcagttttcg gtgtcgcacc
     4381 gcatcaaagc cacacaatct gtacacgttc ggactggacg ggtctgacaa attgtgtcga
     4441 aaaaaccatt accgacgaaa aagtgattgc ttcattgacc aataccgaca tcagcggcaa
     4501 tgtcagcctt gccaatcacg ctcatttaaa tctcacaggg cttgccacac tcaacggcaa
     4561 tctgaccgca ggcggagaca cgcactatga ggttacgcac aacgccaccc aaaacggcaa
     4621 ccttagcctc gtgggcaatg cccaagcaac atttaatcaa gccacattaa acggcaacac
     4681 atcggcttcg ggcaatgctt catttaatct aagcgaccac gccgcacaaa acggcgatct
     4741 gacgctttcc ggcaacgcta aggcaaacgt aagccattcc gcattcaacg gcaatgtctc
     4801 cctagccgat aaggcagtat tccattttga aaacagccgc cttaccggac aaatcagcgg
     4861 cggcaaggat acggcattac acttaaaaga cagcgaatgg atgctgccgt caggcacgga
     4921 attaggcaat ttaaaccttg acaacgccac cattacactc aattccgcct atcgccacga
     4981 tgcggcaggg gcgcaaaccg gcagtgcgac agatgcgccg cgccgccgtt cgcgccgttc
     5041 gcgccgttcc ctattatccg ttacaccgcc aacttcggca gaatcccgtt tcaacacgct
     5101 gacggtaaac ggcaaattga acggtcaggg aacattccgc tttatgtcgg aactcttcgg
     5161 ctaccgcagc gacaaattga agctggcgga aagttccgaa ggcacttaca ccttggcggt
     5221 caacaatacc ggcaacgaac ccgtaagcct cgatcaattg acggtagtgg aagggaaaga
     5281 caacaaaccg ctgtccgaaa accttaattt caccctgcaa aacgaacacg tcgatgccgg
     5341 cgcgtggcgt taccaactca tccgcaaaga cggcgagttc cgcctgcata atccggtcaa
     5401 agaacaagag ctttccgaca aacttggcaa ggcagaagcc aaaaaacagg cgggaaaaga
     5461 caacgcgcaa agccttgacg cgctgattgc ggccgggcgc gatgccgtcg aaaagacaga
     5521 aagcgttgcc gaaccggccc ggcaggcagg cggggaaaat gtcggcatta tgcaggcgga
     5581 ggaagagaaa aaacgggtgc aggcggataa agacagcgcc ttggcgaaac agcgcgaagc
     5641 ggaaacccgg ccggctacca ccgccttccc ccgcgcccgc cgcgcccgcc gggatttgcc
     5701 gcaaccccaa ccgcaacccc aaccccaacc gcagcgcgac ctgatcagcc gttataccaa
     5761 tagcggtttg agtgaatttt ctgccacgct caacagcgtt ttcgccgtac aggacgaatt
     5821 ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg acaagcggca tccgggacac
     5881 caaacactac cgttcgcaag atttccgcgc ctacagccaa caaaccaacc tgcgccaaat
     5941 cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc ctgttttcgc acaaccggac
     6001 cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg cttgcccacg gcgccgtttt
     6061 cgggcaatac ggcatcggca ggttcgacat cggcatcagc gcgggcgcgg gttttagcag
     6121 cggcagcctt tcagacggca tcggaagcaa aatccgccgc cgcgtgctgc attacggcat
     6181 tcaggcacga taccgcgccg gtttcggcgg attcggcatc gaaccgcaca tcggcgcaac
     6241 gcgctatttc gtccaaaaag cggattaccg ctacgaaaac gtcaatatcg ccacccccgg
     6301 ccttgcgttc aaccgctacc gcgcgggcat taaggcagat tattcattca aaccggcgca
     6361 acacatttcc atcacgcctt atttgagcct gtcctatacc gatgccgctt cgggcaaagt
     6421 ccgaacgcgc gtcaataccg ccgtattggc tcaggatttc ggcaaaaccc gcagtgcgga
     6481 atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc ctccacgctg ccgccgccaa
     6541 aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa ttaggctacc gctggtaaca
     6601 ccggatatgc cgaaaggggt ttgacgatgc caccgtgcgc tgtcaaaccc tttttctgcc
     6661 gccgccttgt gcctgctcca ttgtctgata accgtcaaat tgatttttag cccatgtttg
     6721 gtgggtcggg gaaatctata tcttcgtccg tgccgaaata gtctgagacc tttgcaaaat
     6781 tcctttccct cccgacagcc gaaaccccaa cacaggtttt cagctgtttt cagctgtttt
     6841 cgccccaaat acctcctaat tctacccaaa taccccctta atcctccacg gacacccgat
     6901 aatcaggcat ccgggctgct ttttaggcgg cagcgggcgc acttagcctg ttggccgctt
     6961 tcaaaaggtt caaacacatc gccttcagat ggctttgtgc actcactttg ctcag
//
LOCUS       DAXIEB010000101         6902 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000101 DAXIEB010000000
VERSION     DAXIEB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.101"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            127..1032
                     /gene="glyQ"
                     /locus_tag="ACFOA3_001683"
     CDS             127..1032
                     /gene="glyQ"
                     /locus_tag="ACFOA3_001683"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6898661.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1109..1520
                     /locus_tag="ACFOA3_001684"
                     /pseudo
     CDS             1109..1520
                     /locus_tag="ACFOA3_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
     gene            1541..3604
                     /gene="glyS"
                     /locus_tag="ACFOA3_001685"
     CDS             1541..3604
                     /gene="glyS"
                     /locus_tag="ACFOA3_001685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6898662.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSDGEITIENADSYAEQMREQGKVVA
                     SFAERKAAIQTALEGQARRLNASVAADETLLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDNLPNGKVA
                     TAVALADKLEILVGIWGIGLIPTGDKDPYALRRASLGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3621..4667
                     /locus_tag="ACFOA3_001686"
     CDS             3621..4667
                     /locus_tag="ACFOA3_001686"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6898663.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLSEEDFERVRRFLYQCFKRTDTPPAGAWLDFAADGKMRRLFTM
                     RQYFGILHRLLKNR"
     gene            4709..5536
                     /locus_tag="ACFOA3_001687"
     CDS             4709..5536
                     /locus_tag="ACFOA3_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6898664.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGKDAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAIRFFLQHFVMLQPEQIKPIDLMMFSDFFDKEGMPVYQVSPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5548..5643
                     /locus_tag="ACFOA3_001688"
                     /pseudo
     CDS             5548..5643
                     /locus_tag="ACFOA3_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5685..6527
                     /locus_tag="ACFOA3_001689"
     CDS             5685..6527
                     /locus_tag="ACFOA3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6898665.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1808 a         1829 c         1772 g         1493 t
ORIGIN      
        1 ggtttaaagt taatccacta tattatccgt ccgaccgggt ttcaaatcag ccgccttcga
       61 gataaatcta ttcaagcggc tatggaaaca gtaaaataaa acctttattt tatcgaacaa
      121 taagccatgc ttaccttcca acaaatcatc ttcaagctgc aaacattctg ggcagacaaa
      181 ggctgcaccg tcatccaacc cttcgacatg gaagtcggtg caggtacttc ccaccccgcc
      241 acctgcctgc gcgcactcgg gcccgagcct tggtttgccg cctacgtcca acccagccgc
      301 cgccccaaag acggccgcta cggcgacaac cccaaccgcc tgcaacacta ttaccaattc
      361 caagtcgccc tcaaacccgc ccccgccaat atccaagacc tctatctcga ctccctgcgc
      421 gaattgggca tcgatcccaa agtccacgac atccgctttg tcgaagacga ctgggaaaac
      481 cccactctcg gcgcgtgggg tttgggctgg gaagtctggc tcaacggcat ggaagtaacc
      541 cagtttacct atttccaaca agtcggcggc atcgactgca cgcccgtact cggcgaaatc
      601 acctacggca tcgaacgctt ggcgatgtac ttgcaaggtg tagaaaacgt ctacgacctc
      661 gtttgggcaa aaacgctcga cggcaacacc gtcacctacg gcgacgtgta ccaccaaaac
      721 gaagtcgagc aatccaccta caacttcgaa tacagcgatg ccgactggct gctgcgccag
      781 ttcaacgact acgaagcgca agccaaacgc ctgcttgccg aagaaaacgc cgccctcgcc
      841 ctgcctgcct acgagcttgt cctcaaagcg gggcatacgt tcaacctttt agacgcacgc
      901 ggcgcgattt ccgtaaccga gcgggcaact tatattggac gtattcgtgc attgagccgc
      961 gccgtggcgc aaaaatatgt tgaaagccgc gaaaaactgg gcttcccttt aatgaaggca
     1021 aacgccgcct aagggggcga cgatttttta cgttcgggcg gcacgataag gcagccgtcc
     1081 ggaagccatt atatttttta ggaaaatcat gcctgtctat ttccgcaccc caagcaggaa
     1141 acgcctgcac aataatgaac cgccgccagc ataaaaagct gcatttgggc gagtttcaaa
     1201 cattgatttt cggcgttcaa ggcagtttgc accaagctga ggacgatgcc gaagcgttcg
     1261 accgctttat ggacgagctg attgcgtatc ttgcggcaaa cggcttggaa atggcgggcg
     1321 ggggcgattg cggcgatttt tcgtgcctgt ttcaaaaatc cggctcccga ggcttgggcg
     1381 aaaccgagaa aaaagccatt atctcttggc tggcaggtcg cgaagatgtc gccactttgc
     1441 acgccacccc gctggttgac ggtttttacg gggatgacgg ctgggaaaat tacagcgaaa
     1501 cctataaaca ggcagattaa ccaaattcaa agaaagccga atgatgaccc aaaccctttt
     1561 aatcgaactc cttaccgaag aactcccgcc caaagccctg aataatctgg gcaaccattt
     1621 tgccgccgcc gttgccgaag gcttggaaaa agcgcaactg gttgacggtg cagccgaatt
     1681 tacggcttat gcctcgccgc gccgtttggc ggttcaagtc aaaaacgtca aagccgtgca
     1741 agccgatcag aaaatcgtga aaaaaggccc tgccgtggcg aatgccgtaa aagacggtgc
     1801 gccgaccaag gctttggaag gtttcgcacg cggtgcaggg gcgaaaatcg aagacttgac
     1861 catcgtccac gacggcaagc aggacgtgta cgcctacgaa tacgtccaaa ccggcaaacc
     1921 gttgggcgaa cttttggaag acattatcaa tgccgcagtg aaaaaactgc cgattccaaa
     1981 agtaatgcgt tggggcagca gcacatttac cttcgtgcgc cccgtccacg ggctgattgt
     2041 gctgcacggc ggcgacatcg taaacgtcag cgttttgggt ctgcaaagca gcaatcaaac
     2101 cttgggacac cgcttcctct ccgacggaga aatcaccatt gaaaacgccg acagctacgc
     2161 cgaacaaatg cgcgagcaag gcaaagtcgt cgcttcgttt gccgagcgca aagccgcgat
     2221 tcagacggca ttggaagggc aggcacgccg tctgaacgcc tccgttgccg ccgatgagac
     2281 cctgttggac gaagtaaccg cgctggtcga atggcctgtt gttttggaag ccggttttga
     2341 ggaacatttc ctcgccgtgc cgcaggaatg cttgatttta acgatgcagc aaaaccaaaa
     2401 atactttccg ctgctcgacc aaaacggcaa gctgatgaac cgcttcctgc tggtttccaa
     2461 cctgcaaacc gaagacccgt cgcacatcat ccgaggcaac gaacgcgttt tgcgcgcgcg
     2521 cctgtctgat gccgagttct tctacaaaca agaccaaaaa gcgactttgg aaagccgcct
     2581 gccgaaattg tccagcgtgg tgtaccacaa caaaatcggc tcgcaagccg aacgcatcga
     2641 acgcctgcaa agcattgccg cccacatcgc caaagcattg ggcgcggatg ccgccgctgc
     2701 cgaacgcgcc gcgcgtcttg ccaaagccga cttggtaacc gaaatggtcg gcgagtttcc
     2761 cgaactgcaa ggcacgatgg gcaaatacta tgcccgcttg gacggcgaaa ctgaagaaat
     2821 cgccgaagcc gtcgagcaac attaccaacc gcgttttgcc ggtgataact tgccgaacgg
     2881 caaagtcgcc actgccgttg cacttgccga taaactggaa atcttggtcg gcatttgggg
     2941 catcggtctg attccgaccg gcgacaaaga cccctacgcc ctgcgccgcg cctcattggg
     3001 tattttgcgg atgctgatgc agtacggttt ggacgtgaac gaactgattc agacggcatt
     3061 cgacagcttc cccaaaggtt tgctcaacga aaaaacgccg tctgaaaccg ccgacttcat
     3121 gcaggcgcgc cttgccgtgt tgctgcaaaa cgattatccg caagacatcg ttgccgccgt
     3181 actcgccaaa cagccgcgcc gtttggacga tttgaccgcc aaactgcagg ctgtcgccgc
     3241 gttcaaacaa ctgcccgaag ccgccgcgct cgccgccgcc aacaaacgcg tgcaaaacct
     3301 gctgaaaaaa gccgatgccg agttgggcgc agttaacgaa agcctgttgc aacaggacga
     3361 agaaaaagcc ctctttgctg ctgcgcaagg cttgcagccg aaaatcgccg ccgccgtcgc
     3421 cgaaggcaat ttccaaaccg ccttgtccga actggcttcc gtcaagccgc aagtcgatgc
     3481 cttctttgac ggcgtgatgg taatggctga agatgccgcc gtaaaacaaa accgcctgaa
     3541 cctgctgaac cgcttggcag agcagatgaa cgcggtagcc gacatcgcgc ttttgggcga
     3601 gtaaccgttg tacagtccaa atgccgtctg aagccttcag acggcatcgt gcctatcggg
     3661 agaataaatt gcagccttta gtcagcgtat tgatttgcgc ctacaacgta gaaaaatatt
     3721 tcgcccaatc attagccgcc gtcgtgaatc aaacttggcg caacttggag attttgattg
     3781 tcgatgacgg ctcgacagac ggtacgcttg ccattgccaa ggattttcaa aagcgggaca
     3841 gccgtatcaa aatccttgca caagctcaaa attccggcct gattccctct ttaaacatcg
     3901 ggctggacga attggcaaag tcaggaatgg gggaatatat tgcacgcacc gatgccgacg
     3961 atattgccgc ccccgactgg attgagaaaa tcgtgggcga gatggaaaaa gaccgcagca
     4021 tcatcgcgat gggcgcgtgg ctggaagttt tgtcggaaga aaaggacggc aaccggctgg
     4081 cgcggcatca caggcacggc aaaatttgga aaaagccgac ccgacacgaa gatattgccg
     4141 actttttccc tttcggcaac cccatacaca acaacacgat gattatgagg cgcagcgtca
     4201 ttgacggcgg tttgcgttac aacaccgagc gggattgggc ggaagattac caattttggt
     4261 acgatgtcag caaattgggc aggctggctt attatcccga agccttggtc aaataccgcc
     4321 ttcacgccaa tcaggtttca tccaaataca gcatccgcca acacgaaatc gcgcaaggca
     4381 tccaaaaaac cgccagaaac gattttttgc agtctatggg ttttaaaacc cggttcgaca
     4441 gccttgaata ccgccaaata aaagcagtag cgtatgaatt gctggagaaa catttgtcgg
     4501 aagaagattt tgagcgcgtc cgccggtttt tgtatcaatg tttcaaacgg acggacacgc
     4561 cgcccgccgg tgcgtggctg gatttcgcgg cagacggcaa gatgaggcgg ctgtttacca
     4621 tgaggcaata cttcggcatt ttgcaccgat tgctgaaaaa ccgttgaaaa acgccgcttt
     4681 atccaacaga caaaaaacag gataaattat gcaaaaccac gttatcagct tggcttccgc
     4741 tgcagaacgc agggcgcaca ttgccgatac cttcggcagg cacgacattc cgtttcagtt
     4801 tttcgacgca ctgatgccgt ctgaagcact ggagcaggca atggcggaac tcgtacccgg
     4861 tttgtcggcg cacccctatt tgagcggagt ggaaaaagcc tgctttatga gccacgccgt
     4921 attgtggaaa caggcattgg acgaaggtct gccgtatatc gccgtatttg aggatgatgt
     4981 cctgcttggc aaagacgcgg agaaattcct tgccgaagac gcttggctaa aagaacgctt
     5041 tgaccccgat tccgccttta tcgtccgctt ggaaacgatg tttatgcacg tcctgacctc
     5101 gccctccggt gtggcggact actgcgggcg tgcctttccg ctgttggaaa gcgaacactg
     5161 ggggacggcg ggctatatca tttccaaaaa agcgatacgg tttttcctgc aacatttcgt
     5221 aatgttgcag ccggaacaga taaaacctat cgacttaatg atgttcagtg attttttcga
     5281 taaggagggg atgcctgttt atcaggtcag tccggcctta tgcgcccaag agctgcatta
     5341 tgccaagttt cacgaccaaa acagcgcatt gggcagcctg atcgaacacg accgcctcct
     5401 gaaccgcaaa cagcaaaggc gcgattcccc cgccaacaca ttcaaacgcc gcctgatccg
     5461 cgccttgacc aaaatcagca gggaaaggga aaaacgccgg caaaggcgcg aacagttcat
     5521 tgtgcctttc caataaaagg aggagagatg gacatcgtat ttgcagcaga cggcaggatg
     5581 cggcggctgt ttaccttgag gcaatacttc ggcattttgc gccgattgct gaaaaaccgt
     5641 tgaaaaacgc cgctttatcc aacagacaaa aaacaggata aattatgcaa aaccacgtta
     5701 tcagcttagc ttccgccgca gaacgcaggg cgcacattgc cgataccttc ggcaggcacg
     5761 acattccgtt tcagtttttc gacgcactga tgccgtctga agaactgaat cgggtgatgg
     5821 cggaattggt gccgggattg gcaaaacagc acctgttgag cgaagtggaa aaagcctgct
     5881 ttatgagcca cgccgtattg tggaaacagg cattggatga aggtctgccc tatgttgccg
     5941 tatttgagga tgatgtcctg cttggcaaag acgcagaaaa gttccttgcc gaagatactt
     6001 ggttggaaga gcgttttgat aaggattctg cctttatcgt tcgtttggaa acgatgtttg
     6061 cgaaagttat tgtcagaccg gataaagtcc tgaattatga gaaccggtca tttcctttgc
     6121 tggagagcga acattggggg acggctggct atatcatttc gcgtgaggcg atgcggtttt
     6181 tcttggaaag gtttgccgtt ttgccggcag agtggattaa agcggtagat tggatgatgt
     6241 ttacttattt ctttgataag gaggggatgc ctgtttatca ggttaatccc gccttatgta
     6301 cccaagaatt gcattatgcc aagttcctca gtaaaaacag tatgttgggt agcgatttgg
     6361 aaaaagatag ggaacaagaa agaagacacc gccgttcgtt gaaggtgatg tttgacttga
     6421 agcgtgcttt gggtaaattc ggtagagaaa agaagaaaag aatggagcgt caaaggcagg
     6481 cggagcttga gaaagcttac ggcaggcggg tcatatcgtt caaatagttt gtgtaaaata
     6541 tagcggatta aaattagaaa tggatacact gtcattcccg cgaaagtggg aatccagtcc
     6601 gttgagtttc agtcatttcc gataaatcac cgcaacgtta aatttctaga ttcccgcttt
     6661 tgcgggaatg acggcggggg atttctgttt ttccgacaaa ttcctaaaac ttaaaattcc
     6721 gttattcccg cgaaaacaga aaaccaaaaa cagaaaccta aaatctcgtc attcccgcaa
     6781 aaacagaaaa ccaaaaaaca gcaacctaaa attcgtcatt cccgcgcagg cgggaatcta
     6841 gtccgttcgg tttcgcttgt tttaagtttc gggcaacttc cactttgtca ttcccgcgca
     6901 gg
//
LOCUS       DAXIEB010000102         6820 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000102 DAXIEB010000000
VERSION     DAXIEB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.102"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFOA3_001690"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFOA3_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6898666.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDNIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNRHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFOA3_001691"
     CDS             complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFOA3_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6898667.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1827..2060)
                     /gene="rpmB"
                     /locus_tag="ACFOA3_001692"
     CDS             complement(1827..2060)
                     /gene="rpmB"
                     /locus_tag="ACFOA3_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6898668.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2310..3836)
                     /gene="farB"
                     /locus_tag="ACFOA3_001693"
     CDS             complement(2310..3836)
                     /gene="farB"
                     /locus_tag="ACFOA3_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6898669.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3860..5014)
                     /gene="farA"
                     /locus_tag="ACFOA3_001694"
     CDS             complement(3860..5014)
                     /gene="farA"
                     /locus_tag="ACFOA3_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6898670.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARTQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      5408..5452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5456..5929
                     /gene="queF"
                     /locus_tag="ACFOA3_001695"
     CDS             5456..5929
                     /gene="queF"
                     /locus_tag="ACFOA3_001695"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6898671.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPAEYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            6006..6692
                     /locus_tag="ACFOA3_001696"
     CDS             6006..6692
                     /locus_tag="ACFOA3_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6898672.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1711 a         1943 c         1607 g         1559 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caacattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 tgaagcgcgc ggcgaacctg ccgactgttt gggctatctg atttccaacc acaatatccg
      541 ccgcgccgcc tatgaagtcg tatcacaact cgacaatgtc agcattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgtttc tcacaaaccc gccgacaact
      721 cggcatttct tcagatatgc acgactacag ccgaaccatg ttcgtctgcc gcatgaaaca
      781 tactctttcc aatcggcata ccgcttacga atgcttccat tacgggcgca ccatcgcact
      841 gctgccactg gaagaacacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aatctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggg
      961 caggctcggc gatatggaac tggtcagcag catccaccat tacccacttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 gctgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc acttggtgct
     1501 tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag attcgcatac
     1561 ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc gaacagtcgg
     1621 aggctttgct ttcaaactga aattatttca gtttggtttc tttgtacact acgtgtttgc
     1681 gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt ttatttttgg
     1741 tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta tcgcgcattg
     1801 cagtaatcct taactaaggg tttaaattaa gcttcaccgc gagcacgcaa atcagccaat
     1861 acgacatcaa tgcctacttt gtcgatggta cgcaatgcag cgttagaaac gcgcaggcga
     1921 acccagcggt tttcactttc tacccaaaaa cgacgtgatt gcaagttggg caaaaaacgg
     1981 cgtttggttt tgttgttggc gtgcgatacg ttgttgccgg acatcgggcg tttgccggtc
     2041 actttgcaaa ctcgtgccat aatcagtctc caaacttcta aaatagtaaa acgcgattta
     2101 taccacgaaa aacagtgtta gttcaagtgt tttagagaaa aacggctgtt ttacgcggat
     2161 aatccttccg attgtcgcat ctgcggaacg tttgcaggtg cgaaacagat ggggcgcgat
     2221 tatagcggat ttcgggaact gctgcattca aaatgccgtc cgaacatatt tccagtattt
     2281 tcagacggca tttcaagttt tcaaatccct caatgtccac cgccgccgcc gttgtggaac
     2341 ggcggttttg ccagccatat gacgggtatc atgataatga ataacaagct gcccgccata
     2401 aagatttcgt tcgagccgat aataaaaccc tgctgggtaa tggtattgtt gatgatgcct
     2461 agggtttgaa tgtcggaaac gccgtgctgg gacaaatgag cggccgtttc gtgcaatgtt
     2521 gcggaatagg gcgtgatgtg ttcggcaaag cgtgtatggt gcaacgcttc acgccgttcc
     2581 cacagggtac tgacgacgga tacgccgaca ccgcccatca gcacgcgcag gaaattcgac
     2641 aggctgccgg cggcggcaat ctgtccgccc ttcatgtgcg acaaggtgat ggtggtcagc
     2701 ggcaggaaga acatggcaac gcctactccc tgccaaaact gcggccagat gacgttgccg
     2761 atatccatgt cggcataaaa atcggtgcgc caatagaaag tgaaggcaaa ggtcaggaag
     2821 ctggcggtta cgagcaggcg catatcgatt ttattgccga acctgccgat taacggagac
     2881 aggaagacgg gcaggatgcc gacgggcgcg gcggcaagcc ccgcccacgt ggaggtatag
     2941 cccaggttgg tctgcaacac taacggcagc agggtcagcg tccccatata caccataaaa
     3001 cccaacgagg tggcaatgac gccgacggta aaattccgat ctttaaacag cgataaatcg
     3061 acaatcggat atttttctcc caattcccaa acaataaaat acgacaagca caccagtgcg
     3121 actacgccca aggtaatgat ttctccagag gcgaaccagt cgagttcctt acccctgtcc
     3181 aacatcatct gtaacgcgcc gataccgact accatcaatg taagcccgac atagtcggtc
     3241 ggcatcttaa cggtttccgt ttcccgatat ttcaaatgtt tccaggttat ccatgccgat
     3301 atgataccga tagggatatt aatgaagaaa atccaacccc aatgccagtt tccggaaatc
     3361 cagccgccga gtatcggccc gagaacaggg gcaacaacga cggtcattgc ccacaatgcc
     3421 agtgccagcg tccgttttgc gggcggatag gatgccatta acaggctttg cgacaaggga
     3481 atcagcggcc cggcgataaa gccctgcaag atgcggaaaa caaccagcga ctgaaggttg
     3541 ggggcaatac cgcacagcca cgatgtgatg acgaaaccga cagcggcggc ggtaaacaat
     3601 ttgacctcgc cgatgcgttt tgccaaaaag cccgtcagcg gcacggaaac ggcgtttgcc
     3661 acagaaaagg aagtgatgac ccacgtcccc tgagtggttg ccgcaccgag gttgccggcg
     3721 atgacgggga cggcgacgtt ggcgatagtc gtatctaaaa cttccataaa tacggcaagc
     3781 cccaaagaca gcgtaaccca cgccaatgcc gcacccttaa gcggtggata atccattgtg
     3841 tcctccgttt cagacggcat cagggcgcgg attgcccgag gatttcatcg accgtccgat
     3901 cgacttccga ccagtcggta ctttccattt ccggcaatgc cgcacccggc gtttttgaaa
     3961 caggcgcgcc tgcggcggaa gtatccactt taaccgtcat cgacaaaccg atacgcaacg
     4021 gatgcctgtc cacatcttcg cggttcagca cgatacggac ggggacgcgc tgcaccactt
     4081 taatccagtt gcccgttgcg ttttgcgccg gaatcagcga aaacgcgctg cccgtacctg
     4141 ccgaaaaacc tgccaccctg ccgcgataaa caatttgttt gccgtacaaa tcggacacca
     4201 gctcggcagg ctgtccgatt ttcatatgcc gcaactgcgt ctctttaaaa ttagcatcca
     4261 cccacacatc cgacagcggc accaccgcca tcagcggcgc gcctgccgcc acctgctgcc
     4321 cgacctgcac cgaacgcttc gccacctgac cgtccgccgg cgcgcggatt tgcgtccgct
     4381 gaaggttcaa ccacgcatct ttcaacctgc cgattgccgt ctgaaccgcc ggctgttcgc
     4441 gcaaagaaac ctgaccgccc aaagccgcgc gtgccgaaga ttcttccgcc aaagccgctt
     4501 tgaccgccgc ctgcgcctga gacactgccg cacgggcgtg tgccagctct tcggcggaca
     4561 ccgcgcccga ttccgccaaa gcagaccggc ggcgcaaatc atcctgcgtg cgtgccaaat
     4621 ccgcccggcg caaggcaacc tgcgcccccg cctgcgaagt ggcggcattt tgccggcggt
     4681 tttgccgcac cgcctgaacc agctcgtttt ttgcccgctc gtaagccagc acatcattat
     4741 cgtcgtccaa taccgccagc acgtcgcctt ttttcacggc atccgtatcg tcgtgcaaaa
     4801 ccttccgtac cgtaccgccc ttttgcggcg taacctgaac cacgcgtccg gcaacataag
     4861 cgtcttccgt ttcctcttcg tgctgccacc ataaaaaaaa cgccgacccg gcggctgcgg
     4921 cggcaagcgc gaacagcagc gtcaatgccg tcaggcggcg tttgcgtttg gcttgcgtgt
     4981 tttgaagttt tgtttcgtcc gcgtgcgtat ccataagatt gggttccgaa tttcctgatt
     5041 tcatataaac aatgttcttt caataggaag cagattctat atccgcgcaa taacgcaatc
     5101 aagtcagtat tttagatgca atattcaaaa aacaacgctt caggcggcaa aactgaaaat
     5161 atatttcgtt tatatagtgg attaacaaaa atcaggacaa ggcggcgaag ccgcagacag
     5221 tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcgtt
     5281 gagctaaggc gaggcaacgc cgtactggtt tttgttcatc cactatatat gacacggaaa
     5341 acgccgccgt ccaaaccatg ccgtctgaag aaaactacac agataccgcc gcttatatta
     5401 caatcgccgc cccgtggttc gaaaacctcc cacactaaaa aactaaggaa accctatgtc
     5461 ccgcaatacc gaagagctgc aaggcatctc gcttttgggt aatcaaaaaa cccaatatcc
     5521 ggccgaatac gcgcccgaaa ttttggaagc gttcgacaac aaacatcccg acaacgacta
     5581 tttcgtcaaa ttcgtctgcc cagagttcac cagcctctgc cccatgaccg ggcagcccga
     5641 cttcgccacc atcgtcatcc gctacattcc gcacatcaaa atggtggaaa gcaaatccct
     5701 gaaactctac ctcttcagct tccgcaacca cggcgatttt catgaagact gcgtcaacat
     5761 catcatgaaa gacctcattg ccctgatgga tccgaaatac atcgaagtat tcggcgagtt
     5821 cacaccgcgc ggcggcatcg ccattcatcc tttcgccaat tacggcaaag caggcaccga
     5881 gtttgaagca ttggcgcgta aacgcctgtt tgagcacgac gcacaataaa ccatccttcc
     5941 catatttcag acggcattcc ttcgatacga tatgccgtct gaacgccttt ccgaaaggtt
     6001 tttttatgta cgcatttacc gacgcacagc aacagaaggc actcttctgg ctggtgcttt
     6061 ttcatatcct catcatcgcc gccagcaact atctggtgca gttccccttc caaattttcg
     6121 gcatccacac cacttggggc gcattttcct ttcccttcat cttccttgcc accgacctga
     6181 ccgtccgcat tttcggttcg cacttggcac ggcggattat cttttgggtg atgttccccg
     6241 ctcttttgct ttcctacgtc ttttccgttt tgttccacaa cggcagttgg acaggcttgg
     6301 gcgcgctgtc cgaattcaac acctttgtcg gacgcatcgc cttagccagc tttgccgcct
     6361 atgcgctcgg acaaatcctt gatatttttg tattcaacaa attacgccgt ctgaaagcgt
     6421 ggtggattgc cccaaccgca tcaaccgtca tcggcaacgc cttggatacg ttggtatttt
     6481 tcgccgttgc cttctatgca agcagcgatg aatttatggc ggcaaactgg cagggcatcg
     6541 cttttgtcga ttacctgttc aaactcaccg tctgcggtct gtttttcctg cccgcctacg
     6601 gcgtgattct gaatctgctg acgaaaaaac tgacgaccct gcaaaccaaa caggcgcaag
     6661 accgccccgc gccctcgctg caaaatccgt aaaacagccc tgccgggaat cgggacggtt
     6721 gggttttctt ttatttgcaa acactgtcgg aacggataaa tccggattcc cgcccacttc
     6781 gtcattcccg cgaaagcggg aatccggtac gttgggtttc
//
LOCUS       DAXIEB010000103         6738 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000103 DAXIEB010000000
VERSION     DAXIEB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.103"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(231..869)
                     /locus_tag="ACFOA3_001697"
     CDS             complement(231..869)
                     /locus_tag="ACFOA3_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6898673.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(866..1237)
                     /locus_tag="ACFOA3_001698"
     CDS             complement(866..1237)
                     /locus_tag="ACFOA3_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6898674.1"
                     /translation="MKLLFIPLVLFVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(1506..1928)
                     /gene="queD"
                     /locus_tag="ACFOA3_001699"
     CDS             complement(1506..1928)
                     /gene="queD"
                     /locus_tag="ACFOA3_001699"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6898675.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(1935..2453)
                     /locus_tag="ACFOA3_001700"
     CDS             complement(1935..2453)
                     /locus_tag="ACFOA3_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6898676.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(2481..3140)
                     /gene="queC"
                     /locus_tag="ACFOA3_001701"
     CDS             complement(2481..3140)
                     /gene="queC"
                     /locus_tag="ACFOA3_001701"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6898677.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            complement(3344..3530)
                     /locus_tag="ACFOA3_001702"
                     /pseudo
     CDS             complement(3344..3530)
                     /locus_tag="ACFOA3_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            3685..4911
                     /locus_tag="ACFOA3_001703"
     CDS             3685..4911
                     /locus_tag="ACFOA3_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6898678.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(4932..5213)
                     /locus_tag="ACFOA3_001704"
     CDS             complement(4932..5213)
                     /locus_tag="ACFOA3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6898679.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(5292..5426)
                     /locus_tag="ACFOA3_001705"
                     /pseudo
     CDS             complement(5292..5426)
                     /locus_tag="ACFOA3_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5425..5883)
                     /locus_tag="ACFOA3_001706"
     CDS             complement(5425..5883)
                     /locus_tag="ACFOA3_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898680.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(6446..6649)
                     /locus_tag="ACFOA3_001707"
     CDS             complement(6446..6649)
                     /locus_tag="ACFOA3_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898681.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKESLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT         1584 a         1593 c         1701 g         1860 t
ORIGIN      
        1 tccccaaccc tgccatttgc gtgcgccgac aaagggtatc ggcaaggttc aaaagaccat
       61 cggaatttag gaagccgagc ttgtacccaa cggcagcatc ttattcagta aggctccgtc
      121 ctgattgggc ggtggaaatg ccggcatttc cacgcagaag cggcacactt tccccgtatg
      181 tcaaacatcg gtaaaaccgt acgccgtccc tttgagttac actgcttaaa ctactctatt
      241 cccgcccatt tgtgcgtctg cacgctcaac tgccaatgca ccggcgcgtc ggggcggctg
      301 ttcagaatac cgatttggcg gatggtgtcg tagatgttca ttgcaccgtt ttgttcgcag
      361 ggcgaaaggt agtaatgatg cgcacggatt ttgcgttcca tattttcaca gaacgcaacg
      421 acatcgccgt cggcaacaat ccgcacttcg tcggctgttt cgatacagct tttttcgtat
      481 ttggcggcat agcaggcttt ggggctggtg gcgacatagt cgatttgcgg cggcgcggga
      541 ttgagtccgt tggtttcgat acagaggaaa taaccttccg ccttgagcat gtccagcagc
      601 atatcgagat gcggctgtat ggtcggttcg ccgccggtga tgatgatgtt gcgggcggca
      661 taggttttca gacagcctaa gatgtcggac aggctcatca tgccgaatgt caaataatcg
      721 gtatcacacc agccgcaggc gaggttgcat ttgcccaagc ggacgaaaac ggcgggtatg
      781 cccgtgttcc agccttcgcc ttgcaggctt tcaaagattt cgacgatgcg gtattgggga
      841 ttttcggggg caacattgat ttttttcata ccgccaacac cgccgccgct gccaaaaatg
      901 cgggcaaacc ttgtttgacg agtatgcctt tgttgccgga agagaacgcg ccccacgcgg
      961 cggcaatcag gacgaagccg agaaacagta ccgtcgcgcc gtacaccgcg ttatccggcg
     1021 cggcaaaccg cgaccacacc agcccgatgc cgagaaagcc gttatacagc ccttggttgc
     1081 cgaacagggt ctgcacgcga ttttgttcca taaattcata aggcagcttg aacgtttccg
     1141 ccgctttttc gctgggaatc tgcgtcattt caagccaggc gatgtagaaa tgttcggcgg
     1201 cgacgaagag gactaggggg atgaagagga gtttcatggg tattcctttt aatgggtgta
     1261 tttgatatgg aaggtcgtct gaaaaaacgg ggaatccgta caggagaaaa aaccgtttta
     1321 cggtaaattc aaataaggac gctctaaacc gtcctttccg cgcaggcggg aatccagaga
     1381 gttggaattg ctgcaatttt taaatattcc tgataaatca aggtctggat tcccgcctgc
     1441 gcgggaatga cgcggttggg catgtcttat tggaatttac tatatcgata cgttcaagat
     1501 attccctact ccccttcata ctccgcacac gatgtcggcg tttcccacaa tttgacgcgg
     1561 cacacgttca gccccgcgtt tttcagacgg tcgtacattt cgaccgccat attttcggca
     1621 gtggtgcggc agggcaggcg cagggttttc atgttccagc cttccaagag tgcggcgatt
     1681 tgagattcgc gcccgttgcc gccgtggtag atgaaggcgt ggtcgaaggg gtcggtaatg
     1741 tgttgtttga caatggcttt caagtcggta aagtccatca ccataccgtc tttcggcccg
     1801 cctctgacgg ggtcgtctga aacggtgatt tcgagtttgt aggtatgccc gtgcaggttt
     1861 tggcatttgc cgtcatgccc gtcgagcata tgcgaggagt cgaaggtgaa gattttggtg
     1921 attttcatgg gttctcaaat gggcaaatag ccctgaaagc cgatttcggc aggcttgccg
     1981 tgcggattcg gggtcaggcg gatatggaaa ttgccgatgc ggtcgagcgc aagcaggttg
     2041 tccacgtctt ttaagttgtc tttatgttgc tgaacatagc tgtcggtcaa cagggtttgc
     2101 agggcttttt gtttggcttc ggcaggcgtg gcggcggcga acagcccaaa agcgtgcgct
     2161 tcggcgagct gccctgcgcg atagccgccg acattggcaa aatacaagcg cggggcgttt
     2221 tcagacggca ttttcgtttt gggcgtttcg gatacggcga tgtcgtaacc gtccgcccat
     2281 tcgacaacct gccagccgtc gatgtggatt ttgtccgcat cgccgaacca cgcggctttg
     2341 agtgcgggga cggcttcacg gtagtcgtcg cacacagcaa attggatgtc gtgcacttcg
     2401 atattcgacc tgccggcatt gccgccgagg taaaacatat ggagcttggg catatcgtat
     2461 tccttatggt ttgcgcgtgt tcagacggcc tttttacttt ccagatattc cgccagcccg
     2521 cgttcgcgca agatacagct cgggcattcg cggcagccgc cgacgatgcc gttatagcag
     2581 gtgtgggttt gctcgcggat atagtccagc acgcccattt cgtccgccaa cgcccaagtt
     2641 tgcgccttgg tcagatacat cagcggcgtg tggatttgaa aatcatagtc catcgccaaa
     2701 ttaagggtaa cgttcatcga tttgacaaac acgtcgcggc agtcgggata gccggagaag
     2761 tcggtttcgc acacgcccgc gatgatgtgc cgtatcccct gccctttggc gtaaatcgcg
     2821 gcatagagca ggaaaagcgc gttgcggccg tctacaaagg tattcggaac gccgttttcg
     2881 gcagtttcga tggcggcggt gtcgtccatc agggcattgt gcgtaatctg ccgcatcagg
     2941 ctcaagtcga gtacggtttg tttgacgccc aaatcctgcg caatccagcg ggcacgttcc
     3001 agctcgacgg catggcgttg cccgtattgg aaagtaatgg cttggacgtt ttcgcgcccg
     3061 taggtttgga ttgcctgaat caggcaggtg gtcgaatcct gaccgcccga aaagatgacc
     3121 aaggcttttt ggtttgacat atcgaatcct agttttggtg acgcgggagg ttttcgaacc
     3181 gcgaagttgg atagtaaaga cgcggattat acaggcaaac gcgttcagac ggcatctgaa
     3241 cgccatgccg tctgaacacg cggcttatgc cgacttgatg gcgagttccg ccaagcgttt
     3301 gctttgtgcg aaggcgatat tgttttcttc ggcggtcagg acgggagccg ctgtcgggaa
     3361 tatctttttt gacggcttcg cagacggcgg agactttggg gacggtgcgc aatacggctt
     3421 cgcaaccttc aacgctgtcg atgccgcgag cgattcggcg ggcgggattg cgggtgctgc
     3481 cgttttggaa atagtagagg acgaggattt tgagggggat ttgggttcat gggcggattc
     3541 cgttaaaatg gttcggtctt cagacggcat aacagttgtt ccgatgccgt ctgaaacagc
     3601 cgcagttcag gcatttgttc ctgacggcaa aattggctat aacatcgggc atcaatgccc
     3661 aacttactga ccgggcaaga aattatgacc tttttacaac gtttgcaagg tttggcagac
     3721 aataaaatct gtgcgtttgc atggttcgtc gtccgccgct ttgatgaaga acgcgtaccg
     3781 caggcggcgg caagcatgac gtttacgacg ctgctggcac tcgtccccgt gctgaccgtg
     3841 atggtggcgg tcgcttcgat tttccccgtg ttcgaccgct ggtcggattc gttcgtctcc
     3901 ttcgtcaacc aaaccattgt gccgcagggc gcggacatgg tgttcgacta tatcaatgcg
     3961 ttccgcgagc aggcgaaccg gctgacggca atcggcagcg tgatgctggt cgttacctcg
     4021 ctgatgctga ttcggacgat agacaatacg ttcaaccgca tctggcgggt caattcccag
     4081 cgtccgtgga tgatgcagtt tctcgtctat tgggctttac tgacgttcgg gccgctgtct
     4141 ttgggcgtgg gcatttcctt tatggtcggc tcggtacagg atgccgcgct tgcctcaggt
     4201 gcgccgcagt ggtcgggcgc gttgcgaacg gcggcgacgc tgaccttcat gacgcttttg
     4261 ctgtgggggc tgtaccgctt cgtgccaaac cgcttcgttc ccgcgcggca ggcgtttgtc
     4321 ggggctttgg caacagcgtt ttgtctggaa accgcgcgct ccctcttcac ttggtatatg
     4381 ggcaatttcg acggctaccg ctcgatttac ggcgcgtttg ccgccgtgcc gttttttctg
     4441 ttgtggctga acctgttgtg gacgctggtc ttgggcggcg cggtgctgac ttcttcactc
     4501 tcctactggc agggagaagc gttccgcagg ggcttcgact cgcgcggacg gtttgacgac
     4561 gtgttgaaaa tcctgctgct tctggatgcg gcgcaaaaag aaggcaaagc cttgcctgtt
     4621 caggagttca gacggcatat caatatgggc tacgacgagt tgggcgagct tttggaaaag
     4681 ctggcgcggc acggctacat ctattccggc agacagggtt gggtgttgaa aacgggggcg
     4741 gattcgattg agttgaacga actcttcaag ctcttcgttt accgtccgtt gcctgtggaa
     4801 agggatcatg tgaaccaagc tgtcgatgca gtaatgatgc cgtgtttgca gactttgaac
     4861 atgacgctgg cagagtttga cgctcaggcg aaaaaacagc agcaatcttg agtatttttg
     4921 aaactgtatt tttatcctaa agacattttc ctatctgctg attccaccgt ttcctttctg
     4981 tgtgccgcca taatttttat gatagacaag ccattcaagt ttgcattgac tgctttttca
     5041 ttggcaatat ccaaatggct ggtcgctgca tctaaaaata ggattttcgg ttcgcaatat
     5101 aaggcccgcg ccaatacgat gcgttgtttt tgtccgcctg acagtgcgct gcccatatcg
     5161 ccgatcaagg tttcatagcc cataggcatg gcggagattt cttcgtgaat catagcaagg
     5221 caggcgcaat gttcgatttt tcctctatcc ggttccgtat cgaaaaaaca aggggctgta
     5281 ctagattagc cctaaatccc acaccaatcc cgcaggattt taagctgttg agacggtgtg
     5341 ccgaagttaa atcgaaattc gcattctttc aagaacagcg ggaaagattt acgatcgatt
     5401 ccgttgtatt ttcgcaagac gcgtttagtc tagagtctgt atattacatt tatttttagg
     5461 gtctgctagc caatttcttg ttcccttcat tattttatct tctgaaagaa aattattttt
     5521 ttccatgcta ttaatattaa tgatatgatt tttatttaaa ataatttttc catataaaat
     5581 ttccggtttg taatagcaag agtctgaagc aaatccttgc ccgtcaatat aagaaaaata
     5641 aatgttatca tcctccttat aatattctaa aaccaatccg gatattattt tatcaaaatt
     5701 cccttcattt ccatctttca tgactaaaat ataattagca ggccaacctt tatcgtaaac
     5761 atattcaaac tgatagttcc cgaaactctc gatatccgaa ctaaaaaaga agaaaagcaa
     5821 attaatagcc aatacaaaaa caaataagaa caaaaatagc aaaattttca acttagttaa
     5881 caatatttac ctctccttta aattcaatcc tgaaaggcac cccttaccct gggctaccaa
     5941 ttatagtttc catatttcta aatattgttt ttacattact tttttctccc cccaaaggaa
     6001 tgcattttaa aatcatgctt ttcaggtgct aatcgatact taccattaac atctttaatc
     6061 acagatatat ttccatgtat agcccaacgt gaaaaatctg agtattatat acagttatac
     6121 cttactgaaa caaagctagc tgtacatatc tgtaccatat ttttactaaa aacaaaaaat
     6181 atttataatt attaaggata agataaaaac cccaacgcca gattaattct caataatttc
     6241 aaataaaaaa tatttgagat tttttctttt ccaaataata tattgaaata tttaaagtta
     6301 aggtagcttg atttaattag aaaatcaaaa aaaccattaa ttaatttttc ttctttaaat
     6361 attttatatg atattataaa attaaataac attgcgaaaa catatgaaat catcaaaata
     6421 ccaaatatat agtgggaatg cattattatt tagctccttc ttttggctct gacaaattga
     6481 cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc cctgttttcc
     6541 ctgttagtat caatgattct tttcttgcaa gatgtgcacc tgcacaacca cctaatacta
     6601 aaccaccacc tattgtaaat ccttcctgtt gtatctctaa tgataacatt ttcttcttgt
     6661 gccgccctta atacagattg tggaattgga ttgacctgag caggaacttc taaaataagt
     6721 tttccttcaa gctgtctt
//
LOCUS       DAXIEB010000104         6397 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000104 DAXIEB010000000
VERSION     DAXIEB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.104"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            311..2485
                     /locus_tag="ACFOA3_001708"
     CDS             311..2485
                     /locus_tag="ACFOA3_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6898682.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRNLHPTAMPKENEVKLNESDWEATGLPGDPKNL
                     PERQKSVIEKVKTDDGSNIYSSPYLTQSNHQNGSAGNGANQPKNEVEDYKDFKYVYSG
                     WFYKHAAKEIDSDKKKFKLGDDGYIFYHGKEPSRQLPASGKVTYKGVWHFATDVKKSQ
                     KFRDIIQSSKGQGDRYSGFSGDEGEEYSNKNEATLKSGHEGYGFTSNLEVDFGNKKLT
                     GKLIRNNASLNNNTNNDKHTTQYYSLEAQVTGNRFSGTATATDKKENEAQHPFVSDSS
                     SLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKAANGNTAAASGGTDAAASNGAA
                     GTSSENSKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPETSESGSNQA
                     DKGKKGKNGKNGGTDFTYKTTYTPKSDEKDTQAQTGAGGAQAASGTESVNGGQAGTKT
                     YEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKE
                     IPNEQNVVYRGSWYGHIANNTSTSWSGNASNATSGNRAEFTVNFGDKKITGTLTANDR
                     TQPTFTITANIKDNGFEGTAKTADLGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEE
                     LGGWFAYPGDKQTEKATDASGNGNSASSATVVFGAKRQKPVQ"
     gene            2571..5315
                     /gene="tbpA"
                     /locus_tag="ACFOA3_001709"
     CDS             2571..5315
                     /gene="tbpA"
                     /locus_tag="ACFOA3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6898683.1"
                     /translation="MQQQHLFRLNILCLSLMTALPAYAENVQAGQSQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSSKIPTQNGNKKISPNGRTNNPYWVSIGRGNVVTEQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINILGK
                     IDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1844 a         1568 c         1609 g         1376 t
ORIGIN      
        1 tctgtcggtg cggaaactta tcgggtaaaa cggtttcttg agattttgcg tcttggattc
       61 ccacttttgt gggaatgacg cgattagagt ttcaaaattt attctaaata gctgaaactc
      121 aacgcactgg attcccgcct gcgtgggaat gacgggatat aggtttccgt gcggacgcgt
      181 tcggattcac gactgcgcgg gaatgacggg attttggtgt attccctaaa aaaataaaaa
      241 agtatttgca aatttgttaa aaataaataa aataataatc cttatcattc tttaattgaa
      301 ttgggtttat atgaacaatc cattggtgaa tcaggctgct atggtgctgc ctgtgttttt
      361 gttgagtgct tgtttgggcg gaggcggcag tttcgatctt gattctgtcg ataccgaagc
      421 cccgcgtccc gcgccaaagt atcaagatgt ttcttccgaa aagccgaaag cccaaaaaga
      481 ccaaggcgga tacggttttg caatgcgctt caagcggaga aatttgcatc ctacggcaat
      541 gcctaaagaa aatgaggtta aactgaacga gagtgattgg gaggcgacag gattgccagg
      601 cgatcccaaa aacttacccg agcgacagaa atcggttatt gaaaaagtaa aaacagacga
      661 cggcagcaat atttattctt ccccctatct cacgcaatca aaccatcaaa acggcagcgc
      721 cggcaacggt gcaaaccaac caaaaaatga agtagaagat tataaagatt tcaaatatgt
      781 ttattccggc tggttttata aacatgcagc gaaagaaata gattccgata aaaaaaaatt
      841 taagttaggc gacgacggtt atatctttta tcacggcaaa gaaccttccc gacaacttcc
      901 cgcttctgga aaagttacct ataaaggtgt gtggcatttt gcgaccgatg tcaaaaaatc
      961 ccaaaaattt cgcgatatta tccagtcttc aaaaggacaa ggcgacaggt atagcggatt
     1021 ttcgggcgat gaaggcgaag aatattccaa taaaaatgaa gcgactttaa aaagcggtca
     1081 tgaaggttat ggttttacct cgaatttaga agtggatttc ggcaataaga aattgacggg
     1141 taaattaata cgcaataatg cgagcctaaa taataatact aataatgaca agcacaccac
     1201 ccaatactac agccttgagg ctcaagtaac aggcaaccgc ttcagcggca cggcaacggc
     1261 aactgacaaa aaagagaatg aagcccaaca tccttttgtt tccgactcgt cttctttgag
     1321 cggcggcttt ttcggcccgc agggtgagga attgggtttc cgctttttga gcaacgatca
     1381 aaaagttgcc gttgtcggca gcgcgaaaac ccaagacaaa gccgcaaatg gcaatactgc
     1441 ggcggcttca ggcggcacag atgcggcagc atcaaacggt gcggcaggca cgtcgtctga
     1501 aaacagtaag ctgaccacgg ttttggatgc ggttgaattg acactaaacg acaagaaaat
     1561 caaaaatctc gacaacttca gcaatgccgc ccaactggtt gtcgacggca ttatgattcc
     1621 gctcctgccc gagacttccg aaagtgggag caatcaggca gataaaggta aaaaaggtaa
     1681 aaacggtaaa aacggcggaa cagactttac ctacaaaaca acctacacgc cgaaaagcga
     1741 tgaaaaagac acccaagccc aaacaggcgc gggcggcgcg caagctgctt cgggtactga
     1801 gagcgttaac ggcgggcagg caggaacgaa aacctatgaa gttgaagtct gctgttccaa
     1861 cctcaattat ctgaaatacg gaatgttgac gcgtaaaaac agcaagtccg cgatgcaggc
     1921 aggaggaaac agtagtcaag ctgatgctaa aacggaacaa gttgaacaaa gtatgttcct
     1981 ccaaggcgag cgcaccgatg aaaaagagat tccaaacgag caaaacgtcg tttatcgggg
     2041 gtcttggtac gggcatattg ccaacaacac aagcacaagc tggagcggca atgcttccaa
     2101 tgcaacgagt ggcaacaggg cggaatttac tgtgaatttt ggcgataaaa aaattaccgg
     2161 cacgttaacc gctaatgata ggactcagcc aacctttact atcacggcta atattaagga
     2221 caacggcttt gaaggtacgg caaaaactgc tgacttaggt tttgatctcg atcaaagcaa
     2281 taccaccggc acgcctaagg catatatcac agatgccaag gtgcagggcg gtttttacgg
     2341 gcccaaagcc gaagagttgg gcggatggtt tgcctatccg ggcgataaac aaacggaaaa
     2401 ggcaacagat gcatccggca atggaaattc agcaagcagt gcaactgtcg tattcggtgc
     2461 gaaacgccaa aagcctgtgc aataagcacg gttgccgaac aatcaagaat aaggcttcga
     2521 cggcatcgct ccttccgatg ccgtctgaaa gcgaagatta gggaaacact atgcaacagc
     2581 aacatttgtt ccgattaaat attttatgcc tgtctttaat gaccgcgctg cctgcttatg
     2641 cagaaaatgt gcaagccgga caatcacagg aaaaacagtt ggataccata caggtaaaag
     2701 ccaaaaaaca gaaaacccgc cgcgataacg aagtaaccgg tttgggcaaa ttggtcaaaa
     2761 ccgccgacac cctcagcaag gaacaggtac tcgatatccg cgacctgacg cgttacgacc
     2821 ccggcatcgc cgtggtcgaa caggggcgcg gcgcaagctc tggctactcg atacgcggta
     2881 tggacaaaaa ccgcgtttcc ttgacggtgg acggcttggc gcaaatacag tcctacaccg
     2941 cgcaggcggc attgggcggg acgaggacgg cgggcagcag cggcgcaatc aatgaaatcg
     3001 agtatgaaaa cgtcaaagct gtcgaaatca gcaaaggctc aaactcggtc gaacaaggca
     3061 gcggcgcatt ggcgggctcg gtcgcatttc aaaccaaaac cgccgacgat gttatcgggg
     3121 aaggcaggca gtggggcatt cagagtaaaa ccgcctattc cggcaaaaac cgggggctta
     3181 cccaatccat cgcgctggcg gggcgcatcg gcggtgcgga ggctttgctg atccacaccg
     3241 ggcggcgcgc gggggaaatc cgcgcacacg aagatgccgg acgcggcgtt cagagcttta
     3301 acaggctggt gccggttgag gataccaatc ggtacgcaaa tttcatcgtt gaggaagaat
     3361 gcgaaaacag aaattacgaa acgtgtaaag cgaatccgaa aaaagaggtt gtcggcaaag
     3421 acgaacggca aacggtttcc acccgagact acacgggtcc caaccgcttc ctcgccgatc
     3481 cgctttcata cgaaagccga tcgtggttgt tccgcccggg ttttcgtttt gaaaacaaac
     3541 ggcactacat cggcggcata ctcgaacaca cgcaacaaac tttcgacacg cgcgatatga
     3601 cggttccggc attcctgacc aaggcggttt ttgatgaaaa tagaaaatac ggaccgataa
     3661 gaggcaacgg caaatatgcg ggcaaccaca aatacggcgg actgtttacc aacggcgaaa
     3721 acggtgcgct ggtgggcgcg gaatacggta caggcgtgtt ttacgacgag acgcacacca
     3781 aaagccgcta cggtttggaa tatgtctata ccaatgccga taaagacact tgggcggatt
     3841 atgcccgcct ctcttatgac cggcagggta tcggtttgga caatcatttt cagcagacgc
     3901 actgttctgc cgacggttcg gacaaaaatt gccgtccgag tgccgacaag ccgttttcct
     3961 attacaaatc cgaccgcgtg atttacgggg aaagccacag gctcttgcag gcggcattca
     4021 aaaaatcctt cgataccgcc aaaatccgcc acaacctgag cgtgaatctc gggtttgacc
     4081 gctttggctc taatctccgc catcaggatt attattttca aaacgccaaa cgcgcctatt
     4141 cgtcgaaaat acccactcaa aacggcaata aaaaaatcag ccccaacggc agaacaaaca
     4201 atccctattg ggtcagcata ggcaggggaa atgtggttac ggagcaaatc tgccgcttgg
     4261 gcaacaatac ttatacggac tgcacgccgc gcagcatcaa cggcaaaagc tattacgcgg
     4321 cagttcggga caatgtccgt ttgggcaggt gggcggatgt cggcgcgggg ttgcgctacg
     4381 actaccgcag cacgcattcg gacgacggca gcgtttccac cggcacgcac cgcaccctgt
     4441 cctggaacgc aggcatcgtc ctcaaacctg ccgactggct ggatttgact taccgcactt
     4501 caaccggctt ccgcctgccc tcgtttgcgg aaatgtacgg ctggcgggcg ggtgttcaaa
     4561 gcaaggcggt caaaatcgat ccggaaaaat cgttcaacaa agaagccggc atcgtgttta
     4621 aaggcgattt cggcaacttg gaggcaagtt ggttcaacaa tgcctaccgc gatttgattg
     4681 tccggggtta tgaagcgcaa attcaaaacg gcaaagaaac agccaaaggc gacccggctt
     4741 acctcaatgc ccaaagcgcg cggattaccg gcatcaatat tttgggcaaa atcgattgga
     4801 acggcgtatg ggataaattg cccgaaggtt ggtattctac atttgcctat aatcgtgtcc
     4861 gtgtccgcga catcaaaaaa cgcgcagacc gcaccgatat tcaatcacac ctgtttgatg
     4921 ccatccaacc ctcgcgctat gtcgtcggct tgggctatga ccaaccggaa ggcaaatggg
     4981 gtgtgaacgg tatgctgact tattccaaag ccaaggaaat cacagagttg ttgggcagcc
     5041 gggctttgct caacggcaac agccgcaata caaaagccac cgcgcgccgt acccgccctt
     5101 ggtatattgt ggatgtgtcc ggttattaca cgattaaaaa acacttcacc ctccgtgcgg
     5161 gcgtgtacaa ccttctcaac taccgctatg ttacttggga aaatgtgcgg caaactgccg
     5221 gcggcgcagt caaccaacac aaaaatgtcg gcgtttacaa ccgatatgcc gcccccggcc
     5281 gaaactacac atttagcttg gaaatgaagt tctaaacgtc caaacgccgc aaatgccgtc
     5341 tgaaaggctt cagacggcat tttttacaca attcccacca ttttccatca tccccgatac
     5401 accgtaatct cgaatcccgt cattcccgcg caggcgggaa tccagtccgt tcggtttcgg
     5461 tttttttgag gtttcgggta acttctaaac cgtcattccc gcgaaggcgg aaaatcaaaa
     5521 acagaaacct caaatcccgt cattcccgcg caggcgggaa tctagggcgt aaaatctaaa
     5581 gaaaccgttt tatccgataa gtttccgcac cgacagacta gattcccgcc tgcgcgggaa
     5641 tgacgttata tttttcgcat ttgataaaaa agaccgtttg aaattttttc agcggacgca
     5701 aagtattgcg taaaatgctg cttataagaa acgcaaggcg ggcgtagaat gtgcagcgtg
     5761 gacatagggc tggctgtagg gtgggcttca gcccaccaat cccaccgttt ccacctattc
     5821 ccccaactcc gtcaatgtta tccattccgc ccattcccac cgaaaaccga aaccgccgta
     5881 ttcccaaaaa cctttgatgc ggtgaaattg gtgggctgaa gcccacccta cagcccaccc
     5941 tacggctcgc tgaaatttcg tcattcccgc gcaggcggga atccaggtct gtcggtgcgg
     6001 aaacttatcg ggtaaaacgg tttcttgaga ttttacgtcc tagattccca cttccgtggg
     6061 aatgacggga tgcaggtttt cgtgcggacg cgttcggatt cacgactgcg cgggaatgac
     6121 gggatttcag tttgcggatt gatttgaagt tgcaaaatcc caacggatcg gattaccgct
     6181 ttcgcgtttc aaagttacgg cgttatcgga aaaacagaaa atcaaagctg taagaattta
     6241 tttaaaacaa ccgaatttca acggatcgga ttctcgcctg tagggaatga cggcggaagg
     6301 ttttttgtct tttctgacag atgtccgcaa tctgaaatcc tgaccgtggg aacgacggta
     6361 tagtggatta acaaaaacca gtacggcgtt gcctcgc
//
LOCUS       DAXIEB010000105         6335 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000105 DAXIEB010000000
VERSION     DAXIEB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.105"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            520..1629
                     /gene="fic"
                     /locus_tag="ACFOA3_001710"
     CDS             520..1629
                     /gene="fic"
                     /locus_tag="ACFOA3_001710"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6898684.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2643..3455)
                     /gene="murI"
                     /locus_tag="ACFOA3_001711"
     CDS             complement(2643..3455)
                     /gene="murI"
                     /locus_tag="ACFOA3_001711"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6898685.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRSNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3642..4103
                     /gene="tsaE"
                     /locus_tag="ACFOA3_001712"
     CDS             3642..4103
                     /gene="tsaE"
                     /locus_tag="ACFOA3_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6898686.1"
                     /translation="MFDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLKRFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            4100..5350
                     /locus_tag="ACFOA3_001713"
     CDS             4100..5350
                     /locus_tag="ACFOA3_001713"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6898687.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5430..6248
                     /locus_tag="ACFOA3_001714"
     CDS             5430..6248
                     /locus_tag="ACFOA3_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6898688.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
BASE COUNT         1639 a         1714 c         1484 g         1498 t
ORIGIN      
        1 agttttttga gtttcagtca ttcccgataa attgccttag cattgaatgt ctagattccc
       61 gccttcgcgg gaatgacggg atttgaggtt tctgtttttg attttctgtt ttcgtaggaa
      121 tgacggttta aaagttaccc gaaacctcaa aaaaaaggct gtgttttaat atagtgttga
      181 tagacgtact tggctgccat gtattcaatc gtggaaatct atatcttcgt cctcgccgaa
      241 atagtctatg cccgatatac aattttgata cacaaacttg gaaatatggg tatcgtcgcc
      301 ggagcgatag aatgcggaca gttatatata tatacggttt ttttagggaa gcgggcagat
      361 gaagggcgga ttttgaatta accccatgca aattgacttt tgcgggcggt tttacctccc
      421 atatatttac aaaagccaaa tttttaaaca tatatccttg atatatacac ggcgtaaaca
      481 tatactggaa acatctttaa attttttgaa attttaaata tgagcaactg gaaacccgat
      541 attccctata acgatttacc acccctgccg ccaaaacagg atattgaaag caaaaccatc
      601 ctgaaacgtt gtatagccgc ccgtgcatcc cttgcccgtt taaagcaggc ggcagaattg
      661 ataccgaatc aagccatgct gattaacacc cttcctgtta tggaagcccg tgcaagttcg
      721 gaaattgaaa acatcgtaac cacaacggac aagctgtttc aatccctgca aatggatacg
      781 gaacggcaag accctgccac gaaagaagcc ctgcaatacc gcaccgccct gtttgcaggc
      841 tatgaatcac tgacgagccg ccctttatgc acacaaaccg ccatcatggt ctgcaacgcc
      901 atcaagcacc cctacgaaat ggccatccgc aaaacaggcg gcacagccct aaaaggaggc
      961 aacagcggaa atgttgtcta taccccgccc gaaggagaag aaaccatacg cggcaagctg
     1021 gcaaattggg agcggtttat tcacgaaagc ggcgatttag acccgcttat catcatggcg
     1081 gcggcacatt accaatttga agccatccat ccgtttacgg acggcaacgg gcggacgggg
     1141 cgcatattga acagcctgct attgattgaa aaagggcttt tggatttgcc tattttgtat
     1201 ttgagccgct acatcatcga aaacagggcg gactattacc gcctgctttt aggcgtaacc
     1261 gaacggcagg actgggaaag ctggataatc tacatcttag acggcgtagc tgacaccgcc
     1321 gattggacgg tatcgaaaat agatgcgata cgccgcctgt tcgagcagac acggcaacac
     1381 atacggacac acgcacaagg aatctacacg cacgaactgg taaatcttct gtttgagcag
     1441 ccatatacac gcattgccaa cctagaagcg gcagggatag ccaaacggca gacggcctct
     1501 aagtacctga aagagctttc agacataggt gtgctgcaag aaatcgtcat cggcagggac
     1561 aaactattca ttcatccgcg cctaatggaa ctattgcggg gagagggcaa cagctttact
     1621 tcattctaac cccctcttcc cccccccaca tgactaacac gaaacaggga ttttgacacc
     1681 cgaaccgaga ccccttgtat ttcccccgcg aaaagccggc atccgcccgc gtatcatggg
     1741 agcaacaaaa cccctgccta aaattttgac ttgtgcaaat tgggggtata tttgggggta
     1801 tattgaaaaa tggctaaaat aaaatgttta ataaacaaaa tgttgaaact taatttcgat
     1861 agagcatctg catatcgtat tgaggcgttc atggaatttg agaaagctat ttttaaataa
     1921 gaaaaggtaa ctattaaata gctacctttc taaaattaaa tatcaacaca tcgttaaaac
     1981 acagccttta tttttaacaa agttgcaaat gtttttttat ttttttaggg aatacaccaa
     2041 aatcccgtca tttccgcgca gtcgtgaatc cgaacgcgtc cgcatggaaa cctatatccc
     2101 gtcattccca cgaaagtggg aatctagttt tttgagtttc agtcattccc gataaattgc
     2161 cttagcattg aatgtctaga tcccgtctgc tcgggaataa cgggatttga ggtttctgtt
     2221 tttgattttc tgttttcgcg ggaatgacgg tttagaagtt acccgaaacc tcaaaaaaac
     2281 cgaaaccgaa cggactggat tcccgcctgc gcgggaatga cgggtttcga gattacggtg
     2341 ttgttgggaa tgatggaaaa tggtggggat tgtgtaaaaa atgccatctg aagcctttca
     2401 gacggcattt gcggcattcg gacatttaaa aatcccatca ttcccgcgca ggagggaatc
     2461 cgaatacatc tgcacagaaa cctgcatccc gtcattcccg cgaaagcggg aatctagaac
     2521 gtaaaatcta aagaaaccgt tttatccgat aagtttccgc accgacaggt ctagattccc
     2581 actttcgtgg gaatgacggg attttaggtt tctgtaatct cgggcagctt tccgtcatcg
     2641 ttttaaccca aagacaccat ttcaatctgc tccatcgtcc tgcccagaaa acgctcgccg
     2701 atggttctga atttcaaagg aatatcgctg acgtaaaaac ggtagtcggg attgttgttg
     2761 tcggtattga gcaatccttc ctgcgcaagg acgcgtgcgg tttcttcggc agtcgtaatc
     2821 gcggaatcga ccagcgcgac attgcccgcc tccctgccga ttaagggttt gagcaaggga
     2881 aagtgcgtgc agcccaacac cagcgtatcg atgccgtctg caagcaatgg tttgaggtat
     2941 tcgcatacgg tcaggcgggt aacttcgtgt tccagccagc cctcttccac caaagggaca
     3001 agcagcggcg cagcctgcgt gcggacgagc gtatcagggt tgctcctgtg gatggcgcgc
     3061 gcataggcat tgctgttgac tgtcgtattg gtggcgataa tgccgatttt attgttgcgc
     3121 gtcgttgcca gcgcggcttt cgcgccggcg gaaatcacgt ccaacacagg catattgccc
     3181 gtcttttgac ggattttctg ccccgccacc gctgcaatcg tgttgcacgc gataaccatc
     3241 gccttaacat cgtgttccaa taaaaaatcg acaatctgca tcgagaaatt ttcgatggtc
     3301 gccttagatt tcgtcccgta aggcacgcgc gccgtgtcgc cgaaataaat gatgttctcc
     3361 atcggcagcc gctccatcag cgcgcgcaca ttggtcaaac cgccgattcc cgagtcaaaa
     3421 acgccgatgg gtcgctgcct gccgatattt tccatccttt ttccaatccg tcctaaacat
     3481 acaatctgcg tattgtacac gtgccgcttt ttcttgacag ccgttgccgt ctgaaagcag
     3541 gatgcggatt tggctgttat aatgtacggc aatgccgccg ggcgcacggt tttccggccg
     3601 cctgcccctc cgatccgcca cataaaagtt gtaaagcccc tatgtttgat tttccccccg
     3661 tttcccgatt ccttgccgac gaagccgcca cactcgattt gggcgcggcg tggtcttccc
     3721 gtttaaacgc accgctggtc atttacctcg aaggggattt gggtgcgggg aaaaccacgc
     3781 tgacacgcgg catcctgcgc ggattgggtc atcagggcgc agtcaaaagc ccgacttacg
     3841 ccatcgtcga atcttatccg ctgaaacgct tcaccttgca ccatttcgac ctctaccgct
     3901 tctcgttccc cgaagaatgg gaagatgcgg ggcttgacga actgtttgcc gcaaacagcg
     3961 tctgcctgat cgaatggccg caacagggcg gggaatttac gccgcccgcc gacatcaccg
     4021 caacattgac acacgacggc ggcggcagaa aatgcctgct gaccgcccat accgaacgag
     4081 gacgcgaaag cctgccgcta tgaccaaact gacacgaaga caaatcatcc gccgcaccgc
     4141 cggcacactg ttcgccctaa gccccatcgc atccgccgtt gccaaaacgg tacgcgcccc
     4201 gcagtttacc gccgtacgga tatggccgtc gcacacctac acccgcctga cgctggaaag
     4261 cacaaccgcg ctcaaatacc aacacttcac gctcgacaac ccgggcaggt tggtcgtcga
     4321 catacaaaac gcgaacatca ataccgtatt gcacggactg tctcagaagg ttatggcgga
     4381 cgaccctttt atccgcagca tacgcgcggg tcagaacacg ccgaccaccg tccgcctcgt
     4441 catcgatttg aaacagcctt cctacgcgca agttttttcc ctcccccccg tcggcggctt
     4501 taaaaaccgc ctcgtcgtcg atctctatcc gcaagggatg gatcccgacg atccgatgat
     4561 ggcgctgctt aacggcagcc tgaacaaaac ccgtcgcggc tcccctgaag ccgacctcgc
     4621 tcaaaacact cccgcacaaa ccgaacgcgg caaaaacggg cgcagacccg tcattatgat
     4681 cgaccccgga cacggcggcg aagaccccgg cgccgtcagc ccgggcggtt tgcaggaaaa
     4741 acacgtcgtc ctctccatcg cccgcgaaac caaaaaacaa ttggaagcct tgggttacaa
     4801 cgtctttatg acgcgcaacg aagacgtgtt catcccattg ggcgtgcgtg tcgccaaagg
     4861 gcgagcacgg cgggcggacg tattcgtctc catccacgcc gacgctttca ccagcccctc
     4921 cgcgcgcggc acaggggttt atatgctgaa caccaaaggc gcgaccagct ccgccgccaa
     4981 attcttggaa caaacccaaa ataatgccga cgcggtcggc ggcgtaccga ccagcggcaa
     5041 ccgcaatgtc gataccgccc tgctcgatat gacccaaacc gccacgctgc gcgacagccg
     5101 caaactcggc aaactggtgc ttgaagaatt gggcaggctc aaccatctgc acaaaggcag
     5161 ggtggacgaa gccaatttcg ccgttttgcg cgcgcccgat atgccgtcta tcctggtcga
     5221 aaccgccttc ctgtccaacc ctgccgaaga gaagctgctg ggcagcgaat ccttccgtcg
     5281 gcagtgcgcc caatccattg cctcgggtgt ccaacgctac atcaatacat ccgtattgaa
     5341 gcggggttga ttgaaaaggc ggtgtttcaa tatagttgtc ggatgaaggc aggcataaac
     5401 ctacttccgc cccaaaccgc gtttttatga tgaaaggtat ggataaattg agataccaac
     5461 aggatttttt aaatatccgc cccattttta cagccggcga acaagaatac ctgaccgaat
     5521 tgtccgatcg gcttccttta tctgttttaa cggactccgt gcgtaatatt gaagaaatcg
     5581 gtatcgattt tgtgtacagt tctgccaaat tggaaggaaa cacttataat caatacgata
     5641 cgcaggcatt gcttaaactc gggcaaaccg caggcgggaa gttatattcg gatgccgtaa
     5701 tgttaatcaa cctgagggaa agttaccggc acctgctgtc cggtttggac agccccgagc
     5761 cgtttgactg gttggatttc ttaaaaacca cccacagcct gatttccgaa aacttactgg
     5821 aaaaaggctc cggcggcgta gtccgccgcg acagcgttac cataagcggg acagactaca
     5881 cccctttatc taacccgcaa agtttggata cggaattaaa atggttgctt caagaagctc
     5941 ccaaaataga aaatcctttc gaccgcgccg tttatctcca taataatttg gcgtatcttc
     6001 aatattttaa agattgcaat aaacgtactg ccagaaactg catgaccttg tcgctgatgc
     6061 gctccggttt ttttccttgc gtattctccc ccgacagtta tcccgcctat gccgaggctg
     6121 tcgtagccta ttacgaaacc ggcgattacg gtttattcaa gaaatatttt atttcggcat
     6181 acgaaaatac cgtaaataag tacggcccac agcctgatat ggatatttcc cgcaacttct
     6241 ccctttaatt ccaaggggtt gttatccgta tcttccgcac gcggcaaatg cggcggcaca
     6301 gtggacacac ccctccctta ccttcggtta tagtg
//
LOCUS       DAXIEB010000106         6321 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000106 DAXIEB010000000
VERSION     DAXIEB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.106"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..96)
                     /locus_tag="ACFOA3_001715"
     CDS             complement(<1..96)
                     /locus_tag="ACFOA3_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6898689.1"
                     /translation="MRKSRLSRYKQNKLVELFVAGVTARTAAELVG"
     gene            163..465
                     /locus_tag="ACFOA3_001716"
     CDS             163..465
                     /locus_tag="ACFOA3_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898690.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(547..2634)
                     /locus_tag="ACFOA3_001717"
     CDS             complement(547..2634)
                     /locus_tag="ACFOA3_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6898691.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2637..3902)
                     /locus_tag="ACFOA3_001718"
     CDS             complement(2637..3902)
                     /locus_tag="ACFOA3_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6898692.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVENGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLVEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(5124..5360)
                     /locus_tag="ACFOA3_001719"
     CDS             complement(5124..5360)
                     /locus_tag="ACFOA3_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6898693.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT         2021 a         1123 c         1091 g         2086 t
ORIGIN      
        1 gcctactaac tctgctgccg ttcttgcagt tacacctgcg acaaatagct caacgagttt
       61 attttgttta taccggctta gacgactttt tctcataggg ataattctaa cttaatttga
      121 atttccctag ttatctagga cagcccctta aaaaatatcc ctttgaaaga ttttgatgaa
      181 gtttgtgaaa agtgggagaa atcctttaaa gaaaatggaa aagaaattga atataatcaa
      241 aaatattcaa gaatttatgg tgaagttcgt ggagctggct ttaatggcga tatcggagaa
      301 aagctacatc gttgtgccgt gatggaatgg gctgaaaatg agaccgtgaa attagcccaa
      361 aaatgggaac aagagcagaa aaaacaacaa attcaacaga aaaaggaaac tgaaaaatcg
      421 ccaaaacaca aagccagtcg tgatgattgg gaaatggaac gttaaaccgc tattttcatg
      481 tattctatta aattatttga aaaaagactt cactttttgt taaagaaaaa tgaagtcttg
      541 ttctgttcaa cctaaattca gaattttatc tgccgattga attgtttctt ggcggtgtgc
      601 aaccattatt ttcataatac caagactttt taggttatgg ttaatttttt gttcattttc
      661 tacatctaaa tggctacttg cttcgtctaa aaatagaatt ttgggtcgtt tatacaatgc
      721 acgagccaag ataactctct gcttttgtcc acctgataag atatttccca tatcgccaat
      781 caaggtctca tagcccattg gcattttaag tatatcgtca tgtatttgtg ccatttttgc
      841 acattgttca atgagctcca tatttgggct ttcatcaaaa aatgaaatat tttccccaat
      901 agaacctgca aaaaggacat catcttggcg aacaatcccg ctcaatcccc taataaagga
      961 tggagaaact tgatatatat catgcccatt aatactaact gtaccagttt caggttttag
     1021 gctacctgtt aaaatgttta acaaagtgga cttcccccga ccagattgtc ctgttaaaac
     1081 aactgcttca ttatctttaa attccaaatt aatgttttca aaaagatatg gctcattatc
     1141 agcatatctg aatgagacgt tttcaacttt aagaaccagt tgttcattat ctaatttagg
     1201 tatatgatta tacttaataa tttcagtttc tgtttcattt aaagtaatgt cagccaaacg
     1261 ttcagcatga agccctaaca ttttgatttg gatgtattgg tcaacgagag aagctgttct
     1321 gctttcaaat tgccctttat aagccaaaaa agccatcaga acaccgactg taaatgaacc
     1381 atctaaaatt gcgcttgcac caagataaat tatgataata ttttccatgc taaacaacag
     1441 tttatttgaa aattcaaata aagcagagag tttatctgtt gtcagcttgg tattgactgt
     1501 attcacaaat aggctcatcc aagtgccatg tctttgataa tgtttatcaa ataatttaac
     1561 tgattggata ccacgaatgg tttccatgaa atatgagttt tgtttggctt catgaacaat
     1621 attttcttct gttgcatttc ttaatgggta atatgcaagc caacgaatta gtatgtacaa
     1681 aacaagtgtt aaaagaacaa tcagcgataa ttgagtgctg taaattgtca ttaacacgaa
     1741 agtaaaaaca gccattaagc tatttaaaac taaaacaaaa aaagtagaag ttagtgtttc
     1801 ttggatatga tctattgaac caaatcttga aatcacatct cctaaatgtc gtttactgaa
     1861 atagtcatta ggtaagtcaa gtaacctttt aaatatattg gctttccatt gtaaattaag
     1921 agttgtagat aggtgcatac ctacccatgc ttgtaacagg ctaattaact gttgcaggat
     1981 agccagtaaa ccaaatccca aagtaagggt cagtaataaa tttttatcag cagttacaat
     2041 gacatggtct attacccatt gcataaagaa tggactaacc aatgcaaaga cttccaaaga
     2101 aatagctaat ataagcattt gaattaaaga acgttttaag cctgaaatcc cccttaatag
     2161 agataatatt ttgattttct ttgtttcttt tttctcttca aaatgggtat tggggaataa
     2221 ttctagggca atccctgtga atttttgtga aacttcgtcc attttgattt ttcgcatacc
     2281 gacagcaggg tccataatga cgatactgtc tttggaaatg gaacaaagta caacaaaatg
     2341 gtttaagttc caatggagaa tgcagggtag ttgtaaattt gacagctcat ctaactctaa
     2401 acgcaaagct cgtggcgtta aattcatttc attgccaaat ctcatgatgt ctgcaagatt
     2461 tgcgcccttt aatgacaggg tgtatttttg gcgtaacgtt cttaaatcag tgtgaaagcc
     2521 ataataggac aagatggatg tcaggcatgc taaaccacat tcagcaactt ctgtttgcag
     2581 aatgacaggt agctttttgt taaatccaaa ggacagtctt gataaataat ccatttttaa
     2641 ttgatttttc ctgaaatgct gtaaagtggg tcaagtaccc attcgtacaa tttttttcgt
     2701 tcatggagaa tatctgcttc taaaatcatg ccaatttgaa gcggcttgtt ttctccgtat
     2761 gctttaatcg tttgtttttc caatttaact ttcacaagat aggcaggttc atttaataag
     2821 gttgggttag tgaaaatgat acctaaacct gatagctctt gtttaccgag agcagttctg
     2881 gcaactgaaa taatttctcc tgtggcatgt ccaaattttt ggtaagggta cgcttggtaa
     2941 cgtaaaacaa ctttatcttt cggtttaata aaaccaacag ctttactggg tatgtaaaga
     3001 ttggcgacca attcagtttg ttcagggaca atgcttaaca gcaatttaga cggttcaact
     3061 tgttgcccta tatcaacatt aattgttgaa atataacctg atttactagc tcgtatggtt
     3121 tgttcggatt tcaaatcaaa atccaaaatt tcttggttca tttccgtaat cgcacggttg
     3181 agttggctca attcggtttt atggcgttca ggcaggctgc ttaatgtgat tttctgttca
     3241 tcaagttccc tgattgcatt attttgttca cgttttaggc tctccaaacg tgagcgttgt
     3301 tccaataaat ggctttcggc ggtcatctta tcttgttggg atactgcgcc ttgactggct
     3361 aaaaacttgt tcttgttaag ggttttttcc actaaacgaa tttgacgatt ttgccctgta
     3421 atttgctgtt taatattctc taattgattg tttaaacgat gaatgttgtt atgaacattt
     3481 ttttgctcat tttgatgaat gcgctttaaa cgttccaatt cttgtaatgc caaagttttt
     3541 ttaaggttgg cttctgctgc caatttggct tgtacgtttc ctttttcgcc aaaacgcgat
     3601 gtggaaagtt tgaacaatgg ttcgccagct ttgacaaagt taccattttc aacaaattta
     3661 tgcgtaatcg tgccgatatc ggaagagtaa acacgaacca cccccatagt tggaagtaat
     3721 tgaccttcaa cggttgtttt attggtatag ctaccaaaaa tcaaaaagat aatgatacac
     3781 agagcaatga gaaaagcgca aaaagtcaga aataaaaaag agaatggacg ggtcaagata
     3841 acctgacctg tccacttatt ttgttgggct acaaagactt cttttcggaa taagtgggac
     3901 atttattatt atttcttaaa aaaaatggat agaaaaacac caataattaa atacttatgc
     3961 ctaataacat gaatttgcta ttgattgatg tttctatttt aattacatta aatttaacca
     4021 ataatccaga aatggttgca gctaaaatgc aaaagacagt ggcgtaaatg gtttcagtat
     4081 tcataatttt tctctttcaa aattgttcaa tctaaataga aaaaggtaac ttcaatacat
     4141 attgctgtaa ctgaaattac cttttcctta atgatgtaat tatcttatgg cttcatcatg
     4201 ccacgaccga tattgtatcc gccagcagca gagaaaatac cagctccaat tgcgccaacc
     4261 cagcctactg gtcctgtcca cttgttccgt tgagctacaa agacttctgg tctgaaaaat
     4321 gagtgattgt tagacatgtg tacttattta tgtttctttg attgggataa atttgtaaaa
     4381 acaaaaaaaa gcaagttgtt attatagaag aacttaaaat gcttaacata ctcattgagg
     4441 tttgtaccac ataatgagat acgagataat ttgttgggag taaaatcaaa aaaaagatta
     4501 aatatttaaa ataatcacta tattttttca ttatttgttc ctttttgtat ttaagttctt
     4561 atgaaaaact agggtatttt gaatagaaaa tatcaaaata ccctagtttt attaaggaat
     4621 attaccacca acaagtgctt ccatattgta cagcaccagt gattgcacca ccaatagctc
     4681 cgaatttcgc acctggaata gcaccaatac cacctgcaaa tgaaccaaca attgccccac
     4741 cagctgctcc acctaatgca ctaccaatgg tattttttga gaaatcacgc cagttacaag
     4801 cagcaccaga aacttgttaa atttcattca aagtcaaaac ttgcataata ttcacctttg
     4861 ttaagtgtgg ttgagaaaaa agtaaatttt catcgttact cattatttgc aatcatgcat
     4921 atactgtaac caagcactat attcagttgg atcatagttt ttcctactgg gttgtctgat
     4981 atgactattg gcactattgg cacaattggg attagcaact ttattgtgag tacctaatgg
     5041 attgatattt cctgtaaagt ggctattgca tgcgcttaat gagcatagat ttattaatgt
     5101 cagaaaggat aaaacaaaaa atcttaccat tttttactaa taccaaaagt aggtttcgaa
     5161 aactgtacac caagaccttg tacatatcca aattgcttgc taacagaaaa ctctactcca
     5221 ctattgttag gggaatagga agcaccaatt ttaccaacag caaccatatc ttttttacct
     5281 aggttgccgc caccctgtgc agagatatgg aatttgccac ctgacacttc aactaattca
     5341 gaggtgtgta actctttcat gattttctcc tgtaaagaag tatggttgga aatgtaaatc
     5401 agaattaacc tttcttaagt ttaatttaat actttttttg caagtatttt attttaaatt
     5461 aaatcaatat tttaaaaata tttacttgta tactgaaaaa gaaactccta atggtggagt
     5521 ggggcttttg tttaagtaag cattgaaaaa atttaaattt cccattatga ttaacttaat
     5581 gcaaaagctt tttcaggacg tacaaaccag cgaacgggcg ttctgatgtg ggcaggttgg
     5641 ttgtcgctaa ccaatttcag tagctcaatc gtcagattgg tatccgaccc tatcaagcta
     5701 aggagaggct gcggctacta cacaaagcac ctgaattatt gggtgctttt gtctttttat
     5761 ggcattgtac caatttgtgt accacgaggg cttgttcgcc caaacgcggt aacaactgaa
     5821 tacacacttt ccccctcaac ttctttgcgt tctaaaacga ctgtcccaat ccgagaatag
     5881 acaatcatag gacgatgatt gccccttaca tcactaaatt cactaaaaat ttttgcgcct
     5941 gattgaaaaa tcatttccac ataaaagggt tacacctgtt tcatcgtggc ttaatccaaa
     6001 ccgcgttaaa tctgctttgt gttctgccaa aatgtgaatc acaccaaagc cgtgattaac
     6061 ctttttgtgt tcgccgtgtt ttaaaacaat ttgacctgcc gggaagtctg ctctattgtt
     6121 cggacaatag ggggaatata ccaattactt taactacatc attttttgac atttttcttg
     6181 cctttcaatt taaaatccaa aatcatactg ccataattta gcaatccaaa aaaatttagg
     6241 cagcagtaat ggtttcgtat tttagaaaac gaaacttggt ttggtgtttt gaagcatttc
     6301 agtacacaaa catggaattt t
//
LOCUS       DAXIEB010000107         6146 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000107 DAXIEB010000000
VERSION     DAXIEB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.107"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(275..709)
                     /locus_tag="ACFOA3_001720"
     CDS             complement(275..709)
                     /locus_tag="ACFOA3_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6898694.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(712..1374)
                     /gene="exbB"
                     /locus_tag="ACFOA3_001721"
     CDS             complement(712..1374)
                     /gene="exbB"
                     /locus_tag="ACFOA3_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6898695.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1440..2282)
                     /locus_tag="ACFOA3_001722"
     CDS             complement(1440..2282)
                     /locus_tag="ACFOA3_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6898696.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKSEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2537..3175
                     /locus_tag="ACFOA3_001723"
     CDS             2537..3175
                     /locus_tag="ACFOA3_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6898697.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3627..4427)
                     /locus_tag="ACFOA3_001724"
     CDS             complement(3627..4427)
                     /locus_tag="ACFOA3_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6898698.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4563..5069)
                     /locus_tag="ACFOA3_001725"
     CDS             complement(4563..5069)
                     /locus_tag="ACFOA3_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898699.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            complement(5406..6047)
                     /locus_tag="ACFOA3_001726"
     CDS             complement(5406..6047)
                     /locus_tag="ACFOA3_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6898700.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1643 a         1540 c         1268 g         1695 t
ORIGIN      
        1 cggcttcgtt gccttgtcct gatttttgtt aatccactat aaaaattaga aatgcacatt
       61 ttcattattc tcgcgcaggc aggactccag acttacccat ttcagtaatg tttgaaaata
      121 aaagaaaaat cagatgtttg tattcccgcc tgcgcagaaa tggagacggc gctctgtcgt
      181 ctcatttttg ttttaatcaa ctatatatag ctgattaaac ataagaaatg ccgtctgaaa
      241 gactttcaga cggcattcgt tcaagcgtcg aactttattg cgccttggtt tcggttacaa
      301 aaccgatttt ggtgattcct gcctgacggg cggcttctaa agctttgttt acataatcgt
      361 attccaccgc cttgtctgcc gcaatcgcca caatcacgtt ttcattctgc tccttggcgg
      421 ctttcagacg gctttccact tccccgattt ccactttgct tgcagaatcc ccgccgacat
      481 aatagccgcc gttcgcatca atcgtcaggc gcagggggtc tttaggctgt ttgtcctgct
      541 tgtttgtctg ctcggacgcg gtcggcagtt ccaaagggat ggaatgcgtc agcaccggca
      601 tagtaatcat aaacacaatc agcaacacca gcatcacgtc caccaacggc gtaacgttga
      661 tgtcggacat cggagaatcg tcgccggaat tcatcgaacc aaatgccata atcagctatc
      721 cttttgatta agcaggcgga cgtgcaaatc gtgcgccatc gcatccaaat cctgggtcag
      781 tatttttgtg ccgcgattga ggaagttgta tgccaacacc gccggaatcg ccacgaacaa
      841 acccgccgcc gtcgccacca gtgcctcgcc aatcgggccg gcaaccgccg caatactcat
      901 ctgcccgctt tgcccgatat tgatcagggc gtggtaaatc ccccaaaccg tgccgaacag
      961 cccgataaac ggcgcggtcg cgccgatgga ggcaagcgcg gtcatcccgt aatcaaaccg
     1021 gcgcataatc tgcgccatac tgttgcggat ttgaatgacc aaatactcgt tcaacggcaa
     1081 agcctgcgcc agttcggacg cttcgtttcg gcggtagttg cggtaagact gcaatgcctc
     1141 ttgcgccagt ttggacaaag gcgcatcgac ggcgcgcact ttttcgaccg cgtcgttcag
     1201 cgacaaagta tcgcgcatat gccgtttgac ggcggcattc cctttgcgcg cccgatacag
     1261 cttgatgcag cgcaagacaa ccaaacacca cgttacgata ctcatcaaca gcatcaacac
     1321 aaacacaccg atcaggacgg gatcgcccga ttcaaatact aatttcaaat tcataatgat
     1381 tccaacactg aaaaaaccaa tcaaacatcc aagctgccgc aaaccgctgc ggcaaccgcc
     1441 taattcaatt caaacttgac ggggacttta aactccgtcc aggcattggc ttgaaaatgc
     1501 ccgttttgcg ccgccttgcg tgccgcattg tccaaccgag aaaaaccact gcttttcacg
     1561 attttaacgg actcgacatg accgcccgga gaaaccaaaa cgctcaaaac aaccgtaccc
     1621 tgctcgtcat tctccataga aagcgtggga taagccgggc gcggaatgct gccgttggcg
     1681 cgtaaaggat tgcctttgct gctgccggct ccttccccgt gttcgccttt gacaccgccg
     1741 ctacctttac cgctgccttc tccgcgcccc gttccgtctc ctttggtacc ggttccctta
     1801 tcttccccat taccctgctc gctgtctgct ttggcagaag cattgctggg atgttcggca
     1861 ggtttttcag acggcttctc gaccggtttt tccgccggtt tcgggacagg cttcgcttcc
     1921 ggcttaggct ctgatttcgg tttttcttcg ggtttcggct tttcttcagg tttcggctct
     1981 tccttaggct gctgaatatc cgcatccgcc tttttcgtaa ccaccggctt caaaaccggc
     2041 ttgggcggct cgacaggttt gggcggctca ggctcaggtt gcggctcagg cgcagcaggc
     2101 gcacctgcgc cttcgggtgc accgtcccct ccgccaaaat cacccaaatc gacaaattcg
     2161 ataacatttc ctgactctat cacaggcagc ttgtgcgcct gccagagcaa tgccaccatt
     2221 gccaaatgca gcagtgcgac ggaaaacacg actgcggggg ttaaaattcg ttctttatcc
     2281 ataattcggg cataataata gcaacaattc ctatttgcaa cctattttta caatttttgg
     2341 tcatatgaat gtctgttccg ttcacaggca aaccgtgttt aaacgctgta ttacagcaaa
     2401 tcatcagata acgggccggc agaaaaaatg attccgtctg atttcttatt ccaataaaat
     2461 caggttagat gatatattgc cgcttctgtc tgtcagccgt ttcgggctgc acaccacatc
     2521 tgttcaaagg aaaaccatgt ttcaaaattt tgatttgggc gtgtttctgc ttgccgtcct
     2581 gcccgtgctg ctcgccatta ccgtcaggga ggtggcgcgc ggctatacgg cgcgctactg
     2641 gggagacaac actgccgaac aatacggcag gctgacactg aaccccctac cccatatcga
     2701 tttggtcggc acaatcatcg taccgctgct tactttgatg ttcacgccct tcctgttcgg
     2761 ctgggcgcgt ccgattccta tcgattcgcg caacttccgc aacccgcgcc ttgcctggcg
     2821 ttgcgttgcc gcgtccggcc cgctgtcgaa tctggcgatg gctgttctgt ggggcgtggt
     2881 tttggtgctg actccgtatg tcggcggggc gtatcagatg ccgttggctc aaatggcaaa
     2941 ctacggtatt ctgatcaatg cgattctgtt cgcgctcaac atcatcccca tcctgccttg
     3001 ggacggcggc attttcatcg acaccttcct gtcggcgaaa tattcgcaag cgttccgcaa
     3061 aatcgaacct tatgggacgt ggattatcct actgctgatg ctgaccgggg ttttgggtgc
     3121 gtttattgca ccgattgtgc ggctggtgat tgcgtttgtg cagatgttcg tctgactggc
     3181 tttcagacgg cataaacgct ccagaaaacg cggcaggaca tattgccctg ccgcgttttc
     3241 ctgtagtgta atcttatttt tttcatcatt attagaacca ggttgcatga taataccttt
     3301 cattaactga aacactgatt aagaaactcc agtctgtcta atgatgaggt tttcacatcg
     3361 ccaaaacttg ccaatcaaat gctggattta ttgccgtctg agatttggtc aaatccaaag
     3421 gcgacattct tagaccctgt gtgtaaatca ggggtatttt tgcgtgaaat cgtcaaacgc
     3481 ttggatgaag gcttgaccaa tcaaatacca gataaacaaa ctcgcattaa ccacatttta
     3541 aaaaatcaag tttttggaag tactgccacg tatgtaggta gctttgaccg atatttgcat
     3601 aaaaactcct ttgctggtga aaggaattat tttgccaatt ttaaaatatt tctggcacca
     3661 aatagtacaa tgacaaagac aatcatgcca atgattaaat caggatagct agaatgagtc
     3721 aataacgtca atgctcccgc cgctatcaca ccgatattga tgataatgtc attggatgta
     3781 aaaatcatgc tggctttgat atggatttct ttattttgat ttttgctcag tagatataag
     3841 cacagccagt ttgcaatcaa tgccaaaaat gccgtgccaa tcatcagttg ataattgggc
     3901 agctgctcag caccgataaa acgcctaatc acttctatca ccccaaataa cgccaatatt
     3961 atctgcgtta tccccgccaa aaatgccaca cgttttttat acgccagcgt cataccaatg
     4021 gctgatagcg ccaatatata gacaaagctg tccgccagca tatctagact atcagcaatc
     4081 agccccatag aattagcaaa aataccaacc gaacactcta tgataaaaaa cacaaagtta
     4141 atcatgagca cttgatataa taatcttttt tctaagtgct catcaggctt gttaaacact
     4201 atcttatcaa caatcacttc ggtggaaatg atatgactat caaaattaag cggttcaagt
     4261 acttgtaaaa tcgttgtatc ttgattatcg tgatagacgg ttaagcaccg cccagcaata
     4321 tcaaactgta attcataaat atcagacaca tcttttaaac gcatgcgaat gagctgttct
     4381 tcggacgggc agtccatttt ggtaatgtta aaaatggtct ttttcatcta tttagttcct
     4441 tgttttgatc aggttggctc aaataaatct gtgtttatat tgctgcttgg taatttttgg
     4501 atggtttgag taaattgatt aggttaaaat ttacctttgg aagtaccgcc acgcataata
     4561 gtttagatat gtttataatc tctggataaa aaaacgtaat aagtgtttgc tggataacaa
     4621 agtccaaacc aatagcagat aaaataaggc atccaccccc tttcttcatt aaggataaga
     4681 gatatcgaga aacaaatcat aactaaacag aaaaaatttt ggagataaag ccatttcatt
     4741 cccctattca agaatctggc caagataggt attttgtata ctctagaaaa gaaaggcatt
     4801 tccaagggaa acatatcaga taaaaaattt tgtttatttt ttactataga tagaacccta
     4861 tttctcaaga taaatccaaa tgcaatgcca attatcatta ttaataaaca aaggatagta
     4921 taactatgga taaccttcat taaacaaaaa gaagaaatgg cagataaatt acatacgata
     4981 tagtggaata taaaatattt acggtctaaa ccttgttcag ttgcaatttt tttatactta
     5041 ccttgcgcaa aaaaatcaaa gaaatccatt aaactatctt tcacattaga aatttaaaag
     5101 ctaaataata cgacaaacaa tgtgaagtac tattcatggt ttatttaaaa aataatacta
     5161 ttctgaacat tatttagata cagaaattaa caaattagaa ctaaacaagc ttttaaatac
     5221 tttaatttta ttggaaagct ataaaaggaa ctataacttt acacactagt cacttctttt
     5281 taagaggcaa aagggattgg gaaggtcgtc ttggagataa gcactggtat ttcggccaat
     5341 ggtaaataga gtttacctca aatagggtag aacctccttc atctgtcagt taataacagc
     5401 cacttttaca atgccctgtc gaaataaagt ggcacgcccg atttttcact cataccgatt
     5461 aaataatcca tcgtcttccg agggaacgcg atgccgcgca ccacggtcag attcctcaaa
     5521 acggggaaaa ccaaaatatc ctccataccg attccgccgt tgatgccgtc tgaagcaccg
     5581 tccatcaaat tttccaactc ttgcaaatct gcgtttatcc gttcgaggta ttgggcggtt
     5641 ttattcaaat tggcggaaaa gctgccgatg cttttctctt ttttgtctgt aaaatatttc
     5701 accgcttccg gcgttgcaaa ttcaggcagc ccgattttga tcacgcgcgg ctgcaccagt
     5761 ttgtcgttgt atccgcccac cttgtccagc cacgcccgta tctcggggcg gacttcgtct
     5821 ttcagacggt cttcgcggtc gaaatgccgc acaatgtcca aactctcgcc cataaacgaa
     5881 ccgtcttctt tttgcaggac gggcacttgt ttcgcaccga tcataccgat cggcgttgcc
     5941 tcgtcgtcgt ttgccagcac ggcttcttca acgtccgcgc caaacagccc ggcagccatc
     6001 cgcgcacgca cgcaaaacgg gcaatggtcg taaatataca gtttcatcaa aatattcctc
     6061 gtcaacctgt cggtaccggc taccttaaca ccccgcgcca cccgaaacaa gtttatcttc
     6121 ccgcctatgc accgtaaata aataag
//
LOCUS       DAXIEB010000108         5992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000108 DAXIEB010000000
VERSION     DAXIEB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.108"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            59..505
                     /gene="yciA"
                     /locus_tag="ACFOA3_001727"
     CDS             59..505
                     /gene="yciA"
                     /locus_tag="ACFOA3_001727"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6898701.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(556..1377)
                     /locus_tag="ACFOA3_001728"
     CDS             complement(556..1377)
                     /locus_tag="ACFOA3_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6898702.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQTLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1496..1954
                     /locus_tag="ACFOA3_001729"
     CDS             1496..1954
                     /locus_tag="ACFOA3_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6898703.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAASFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2098..3234)
                     /gene="lst"
                     /locus_tag="ACFOA3_001730"
     CDS             complement(2098..3234)
                     /gene="lst"
                     /locus_tag="ACFOA3_001730"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6898704.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKNQSHSKSK"
     gene            complement(3436..3615)
                     /locus_tag="ACFOA3_001731"
     CDS             complement(3436..3615)
                     /locus_tag="ACFOA3_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898705.1"
                     /translation="MCVAIMAYFGGFVRALFDLGVFERGGVKGRGEMVFPLLAAVSEV
                     LCLMCGGIFSVKLIL"
     gene            3669..5894
                     /locus_tag="ACFOA3_001732"
     CDS             3669..5894
                     /locus_tag="ACFOA3_001732"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6898706.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELRAPVALK
                     AGEITDATVMSKKALLAFLAEQVQDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTSMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1600 a         1759 c         1335 g         1298 t
ORIGIN      
        1 aacgccgtac tggtttaaat ttaatccact atatatttca ataaaacaag gaaactttat
       61 gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa ctgctcctgc gtaccgtcgc
      121 tatgccgcgc gataccaatc ccaaccaaga cattttcggc ggctggatta tgtcgcagat
      181 ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg ggacgcatcg ttaccgtcgc
      241 cgttcaagaa atgaacttca tccgcccggt caaggtcggc aacgtcgtct gctgctacgg
      301 gcattgcgtc cgcgtgggca atacttccct ccagcttaaa atcgaagtct gggtgaaaac
      361 tttgatgaac gattgcgtta ccgaagaccg ctacctcgta accgaggcgg tgttcactta
      421 tgttgccatc gatgcggaag gcaatccgcg cccgattccg aaagaaggca accccaaatt
      481 gtcgggctta ttgcctactc cgtaaaaata cccgtaaaaa tgccgtctga aactgtcttc
      541 agacggcatt ccgtatcatt tgaaccaaga cctgatgcgg gaaaacagcg acatactctg
      601 tttttcaaag tcttcccgca cttcgggcac ggcttttttt atcactttca ttcctgcaaa
      661 agtctgcgcc acgggcataa tttcaatcgt gttcacattc atatgcgccg gccggcggta
      721 cagccacaat gcggtttccg caatatcttc ggggcggata aattccacac cctcatacac
      781 gcccgccgcc ctctcgtcat cgcctttgaa gcgcacgttg gaaaactccg tattgccgca
      841 caaacccggc tcgatattgg taacgcggat gttcttatcc gccaactccg cgcgcaaatt
      901 caggctgaac tggcgcacaa acgccttggt cgccccgtaa acgttgctgc cggcataagc
      961 ataattgcct gcaatcgaac ccaaattcat cacataaccg ccgccgcgtt ccaccatttg
     1021 cggcaaaatc ttgcgcgtca ggaacgtcaa acccaaaaca ttggtttgaa tcattgtttc
     1081 ccaatcttca aaatccgcct tgtccgccgt gtccaaaccc aaagccagcc cggcattgtt
     1141 gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg tttaaggcgt tttccaccga
     1201 ctcgcgtcgc gacacgtcca tttccaaagg gtaaaacaaa gcacccaatt catccgccaa
     1261 ggtctgaagc ctgtccgcac ggcgcgccgc accgataacg cggtatcccg ccccgacaaa
     1321 cgcgcggcac atcgcttcgc cgaatcctgc cgaagcaccg gtaattaaaa cagccattgt
     1381 ttttcctttc tttctcgttt cccaaacctg tttcaggtat cataacgctc ttcagacggc
     1441 atcgcgccgg ctgaaaatga caacaaccat accaccacga caggacacat tcaatatgaa
     1501 aacccaaatc agccttgctg ctgccgccat cacactgctc ctttccgcct gcggaggcag
     1561 cggaatacct tcccaaccca aaggtgaaat ttccgaaaac cgcaccgccg cgttcaaatc
     1621 tatgatgccc gacttctcgc gtatgggcaa aatggtcaaa ggcgaagagc cttacgatgt
     1681 cgaaaaattc aaacaggcgg cagcgtcgtt tgccgacagc agcaagaaac cgttcacact
     1741 ttttgagtcc gacccgcaag gtaacggccg cgccctgcca gccgtttggt cggatggtgc
     1801 aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc gaaaaactca acgccgcagc
     1861 acaaaccggc aaactggatg aaatcaaagc cgcctacggc gaaaccggcg caagctgcaa
     1921 atcctgccac gacagtttcc gcgcgccgga ataacatccg aaacagaacg atgccgtctg
     1981 aagccttcag acggcatttt ctttgtcatt tcagcacacg caaagcaatc agaaattatc
     2041 gtcaattcaa atgaaaaaac gctatcctta tttttacccc acgcactccg ccattctcta
     2101 ttttgatttg ctatgcgatt gatttttatc gtcaaatgtc aaaatcggga tgccggattg
     2161 ggtaaacagg gcatacaccg gcttgagcca ataatcttcc ggaagggaag ccggtttcaa
     2221 tgcgtaaacg tgcacattgg gaaaatcctt catcgtcaac gccgcgccgc tgaaaaaggt
     2281 ataaatttca tacctcgtat gcgggttttt cttaatctcg cgcaaaatat agtcttcgat
     2341 gacatagggc gaatttaatg tggttacgcc ggaaagcccg taggtttggc gggggtgcgg
     2401 tgcgacatat tgtatgttga agttttttgc cgccttttcc gaaatttcct tcatctcctt
     2461 gtcgggcgaa cccaaaagta tccgcaccgt gccgcccgtt tcgtcccccg ccttcagttc
     2521 ggacgcatcg aacagcggca ggtaagtcat cttgcggcgg ccgtcgtcca taatgttttt
     2581 caaacccttg aatatcgtgt aatgctcgtc ggaagcatta cgggttttgg cgatgctcca
     2641 atctccgatc atcatccggg caaaattccg cttgatcgtc ccgtttacag aaaactcatc
     2701 gcccaaatag ctgctgcttt gaattaaatt gcctgtcccg tcgtcaaagg ttttgatttc
     2761 cgcatccggg taagtgctca aaaaggcggc aatgctgact ttttccaaac ttgccaaata
     2821 aatccgcttg accttcggca gcagcattgc cttcaccttc agctccgcca tcgtcggaat
     2881 gaaattaaac gatttgttca aaccgtaggg caggtggaaa aaatacgccc gctccgcctt
     2941 atcctttatc tgattgaaat aataatcgta tttttcattc ctgttttcag acatcagcac
     3001 cacataaaac cgctcccccg gatgctgcgc cataatcctt tccgccacct tcatctgcaa
     3061 tatggtatag cagaaaatca gattgaccgg ttccccctct tcattgaaga gtttgtcctt
     3121 cagcagggaa accgcattcc tttccccctg atttacccgg tcaaatgtat aaaatatccc
     3181 gaaacaaaaa acaatcagac acaacacggt caaacaagcc tttttcaagc ccatatccct
     3241 aaaactccat tccgacaaat taaacatacg ccccgagtgt cccgggcgga aaaatgcgga
     3301 attttagcag atattccgac agttaccgaa tattatagtg atttaaattt aaatcaggac
     3361 aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac gccgtactgg
     3421 tttaaattta atccactata aaatcaattt caccgaaaat atgccgccgc acatcaggca
     3481 cagcacctcc gacaccgccg ccaaaagcgg gaaaaccatt tccccccttc ctttcacgcc
     3541 gccgcgttca aacacgccaa gatcgaacaa agcgcgaaca aagccgccaa aatatgccat
     3601 aatagccacg cacattcccc gcgcaatgtg ttccaaaaga aaaaaccaac ctacaagaga
     3661 ataatcctat gactcaaaaa tccaccattg tttataccca taccgacgaa gcccccgcgc
     3721 tggcgaccca atcgctgctg ccgattgtgc aggcttttgc ccgccacgcc gatattgatg
     3781 tcaaaactgc cgacatttcc ctgtccggcc gtattttggc ggcattcccc gaatatctga
     3841 ccgaggcgca gcgcgtaccc gacgcgcttg ccgaattggg cgaactggtg aaacagcccg
     3901 atgcaaacgt aatcaaactg ccgaacatca gcgcgtccgt acctcaactg accgccgcga
     3961 ttaaagaatt gcagtctaaa ggctttgccg ttcccgacta tcctgccgac ccgcaaaccg
     4021 atgaagaaaa agccgtacgc gaacgctacg accgcatcaa aggcagcgcg gtcaaccctg
     4081 tcctgcgtga aggcaactcc gaccgccgcg cccccaaagc ggtgaaaaac tttgccaaaa
     4141 aaaatccgca cagcatgggc gcgtggacaa aagactccaa aacccacgtt gccaccatgc
     4201 aaagcggcga ctttttccat aacgaacaat ccgttaccgt acccgatgca acttccgtat
     4261 ccatcgtgtt taccgacaaa caaggcaaca aaaaagagct gcgcgcgccc gtcgccctga
     4321 aagcgggcga aatcaccgac gcgaccgtga tgagcaaaaa agccctgctc gccttccttg
     4381 ccgaacaagt gcaagacgcg aaagcaaaag gtgtattgtt ctcgctgcac atgaaagcca
     4441 ccatgatgaa agtgtccgac ccgattatct tcggacatgc cgtcaaagta ttcttcgcgc
     4501 ctgtatttga aaaattcggc gacaaactgg ctgccgcagg tgtcaacgtc aacaacggct
     4561 tcggcaacct gcttgccaat ttggacaaac tggatgcgga cacccgcgct gccgttgaag
     4621 ctgaaatcgc tgccgtttac gccgccaacc ctgatttggc tatggttgat tccgacaaag
     4681 gcattaccaa cctgcacgtt cccagcgatg tcatcgtcga tgcttctatg cctgcgatga
     4741 ttcgtaactc cggccgcatg tgggacaaaa acggcaaagc gcaagacacc aaagccgtga
     4801 ttcccgacag cagctacgct ggcgtttacc aagcaaccat cgatttctgc cgcgaacacg
     4861 gcgcgtttga cccgacatcc atgggtaccg ttcctaacgt cggtctgatg gcgcaggcag
     4921 ccgaagaata cggttcgcac aataaaactt tcgaaatcga agccgacggt caggttcaag
     4981 tcattgatgc ggaaggaaaa gtcctgatgc agcacgacgt tgaagcaggc gacatctggc
     5041 gtatgtgcca aaccaaagac gctccggtca aagactgggt gcaactcgcc gtcaaccgcg
     5101 cccgcctgag caacacgcct gccgtgttct ggctcgatga aaaccgtccg cacgacaaga
     5161 gcctgctcgc caaggttaaa gcctaccttg ccgaactgga taccaatggc ctcgacatcc
     5221 gcgtcctcgc tcctgaagaa gccgccaagt tcagcttggg tcgtctgaaa aacggcgaag
     5281 acaccatctc cgtaaccggt aatgtcttgc gcgactacct gaccgacttg ttcccaattt
     5341 tggaactcgg cacaagcgcg aaaatgctgt ctatcgttcc attgatgaac ggcggcggta
     5401 tgtttgaaac cggcgcgggc ggttctgcac cgaaacatgt tcaacaattc ctcgaagaaa
     5461 accacctgcg ttgggactct ttgggcgaat ttctcgcact cgccgtatcg tttgaacatc
     5521 tggcgcaaaa aaccggcaac accaaagccc aagtcctcgc cgacactttg gatgcagcca
     5581 ccgaaaaact gctgttgaac gacaaatcgc ctaaacgtaa agccggcgaa ctcgacaacc
     5641 gcggcagcca tttctacctc accctctact gggcgcaaga attggcggcg caagacaaag
     5701 atgccgaact gaaagccgca tttacgccat tggcagccgc tttgaccgcc gacgaagcga
     5761 aaatcgttgc cgagctttcc gccgtacaag gcaaagcggc cgacatcggc ggctactacg
     5821 ccgccaatcc tgaaaaagcc gcacaagtga tgcgtccgag cgtaaccttt aatcaggcac
     5881 taaacgcctt ataagcacaa aggtaaaaat gccgtctgaa cattcgtcgt tcagacggca
     5941 ttagttatcc ctatctgcct gattatgatt aaacgccgca cccattggat at
//
LOCUS       DAXIEB010000109         5955 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000109 DAXIEB010000000
VERSION     DAXIEB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.109"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..410)
                     /locus_tag="ACFOA3_001733"
     CDS             complement(<1..410)
                     /locus_tag="ACFOA3_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6898707.1"
                     /translation="MLCPEKMSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVG"
     gene            complement(446..559)
                     /gene="rrf"
                     /locus_tag="ACFOA3_001734"
     rRNA            complement(446..559)
                     /gene="rrf"
                     /locus_tag="ACFOA3_001734"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(656..3547)
                     /locus_tag="ACFOA3_001735"
     rRNA            complement(656..3547)
                     /locus_tag="ACFOA3_001735"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3953..4028)
                     /locus_tag="ACFOA3_001736"
     tRNA            complement(3953..4028)
                     /locus_tag="ACFOA3_001736"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3993..3995),aa:Ala,seq:tgc)
     gene            complement(4034..4110)
                     /locus_tag="ACFOA3_001737"
     tRNA            complement(4034..4110)
                     /locus_tag="ACFOA3_001737"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4074..4076),aa:Ile,seq:gat)
     gene            complement(4212..5752)
                     /locus_tag="ACFOA3_001738"
     rRNA            complement(4212..5752)
                     /locus_tag="ACFOA3_001738"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1297 a         1749 c         1277 g         1632 t
ORIGIN      
        1 ccgaccgaag cctgatcctt cagcttcgcc agcaccgcag ggccgatgcc ctttaccttg
       61 gtcaaatcgt ccacagactt gaacgcaccg ttttgcgcac ggtattccgc aatggccttc
      121 gccttcgccg ggcctatgcc tggcagcgcc tccaactcct gcagcgaagc cgcattgatg
      181 tttaccgccg caagggagaa ggcgcaggag aacagcatac agaacagcac gaacattttc
      241 ttcatggttt ttcctttaag ggttgcaaac aataaaccgc atcttgcgac gataaaacga
      301 gtcattctaa aatgaatatc ccaaagtttc aagccgttcc tccgcaaacc cgaccggaca
      361 ccgtacggat gccgtcccgc catcaccgac attttttccg ggcaaagcaa aaacccccga
      421 ataatcgggg gttttctgaa tgggtgtttg gcagtgacct actttcgcat ggaagaacca
      481 cactatcatc ggcgctgagt cgtttcacgg tcctgttcgg gatgggaagg cgtgggacca
      541 actcgctatg gccgccaaac ttaaactgtt acaaatcggt aaagccttaa tcaatatatt
      601 cggtaatgac tgaatcagtc agtaagcttt tatctcttga agttcttcaa atgatagagt
      661 caagcctcac gagcaattag tatgggttag cttcacgcgt taccgcgctt ccacacccca
      721 cctatcaacg tcctggtctc gaacgactct ttagtgcggt taaaccgcaa gggaagtctc
      781 atcttcaggc gagtttcgcg cttagatgct ttcagcgctt atctcttccg aacttagcta
      841 cccggctatg caactggcgt tacaaccggt acaccagagg ttcgtccact ccggtcctct
      901 cgtactagga gcagcccccg tcaaacttcc aacgcccact gcagataggg accaaactgt
      961 ctcacgacgt tttaaaccca gctcacgtac cactttaaat ggcgaacagc catacccttg
     1021 ggaccgacta cagccccagg atgtgatgag ccgacatcga ggtgccaaac tccgccgtcg
     1081 atatgaactc ttgggcggaa tcagcctgtt atccccggag taccttttat ccgttgagcg
     1141 atggcccttc catacagaac caccggatca ctatgtcctg ctttcgcacc tgctcgactt
     1201 gtcggtctcg cagttaagct accttttgcc attgcactat cagtccgatt tccgaccgga
     1261 cctaggtaac cttcgaactc ctccgttacg ctttgggagg agaccgcccc agtcaaactg
     1321 cctaccatgc acggtccccg acccggatga cgggtctggg ttagaacctc aaagacacca
     1381 gggtggtatt tcaaggacgg ctccacagag actggcgtct ctgcttctaa gcctcccacc
     1441 tatcctacac aagtgacttc aaagtccaat gcaaagctac agtaaaggtt cacggggtct
     1501 ttccgtctag cagcgggtag attgcatctt cacaaccact tcaacttcgc tgagtctcag
     1561 gaggagacag tgtggccatc gttacgccat tcgtgcgggt cggaacttac ccgacaagga
     1621 atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct tcgatccgat
     1681 gctctcacat cttcaattaa ccttccagca ccgggcaggc gtcacaccct atacgtccac
     1741 tttcgtgtta gcagagtgct gtgtttttaa taaacagtcg cagccaccta ttctctgcga
     1801 ccctccgggg cttacggagc aagtccttaa ccttagaggg cataccttct cccgaagtta
     1861 cggtatcaat ttgccgagtt ccttctcctg agttctctca agcgccttag aattctcatc
     1921 ctgcccacct gtgtcggttt gcggtacggt tcgattcaaa ctgaagctta gtggcttttc
     1981 ctggaagcgt ggtatcggtt gcttcgtgtc cgtagacact cgtcatcact tctcggtgtt
     2041 aagaagaccc ggatttgcct aagtcttcca cctaccggct taaacaagct attccaacag
     2101 cttgccaacc taaccttctc cgtccccaca tcgcatttga atcaagtaca ggaatattaa
     2161 cctgtttccc atcgactacg catttctgcc tcgccttagg ggccgactca ccctacgccg
     2221 atgaacgttg cgcaggaaac cttgggcttt cggcgagcgg gcttttcacc cgctttatcg
     2281 ctactcatgt caacattcgc acttctgata cctccagcac actttacaat gcaccttcat
     2341 cggcctacag aacgctcccc taccatgccg gtaaaccggc atccgcagct tcggttatag
     2401 atttgagccc cgttacatct tccgcgcagg acgactcgac cagtgagcta ttacgctttc
     2461 tttaaatgat ggctgcttct aagccaacat cctggctgtc tgggccttcc cacttcgttt
     2521 accacttaat ctatcattgg ggaccttagc tggcggtctg ggttgtttcc ctcttgacaa
     2581 cggacgttag cacccgctgt ctgtctcccg aggaaccact tgatggtatt ctgagtttgc
     2641 catgggttgg taagttgcaa taacccccta gccataacag tgctttaccc ccatcagtgt
     2701 cttgctcgag gcactaccta aatagttttc ggggagaacc agctatctcc gagtttgttt
     2761 agcctttcac ccctatccac agctcatccc cgcattttgc aacatgcgtg ggttcggtcc
     2821 tccagtacct gttacggcac cttcaacctg gccatggata gatcactcgg tttcgggtct
     2881 acacccagca actcatcgcc ctattaagac tcggtttccc tacgcctccc ctattcggtt
     2941 aagctcgcta ctgaatgtaa gtcgttgacc cattatacaa aaggtacgca gtcacaccac
     3001 tagggtgctc ccactgtttg tatgcatcag gtttcaggtt ctgtttcact cccctcccgg
     3061 ggttcttttc gcctttccct cacggtactg gttcactatc ggtcgatgat gagtatttag
     3121 ccttggagga tggtcccccc atattcagac aggatttcac gtgtcccgcc ctacttttcg
     3181 tacgcttagt accgctgttg agatttcgaa tacgggactg tcacccacta tggtcaagct
     3241 tcccagcttg ttcttctatc tcgacagtta tcacgtacag gctcctccgc gttcgctcgc
     3301 cactacttgc ggaatctcgg ttgatttctt ttcctccgga tacttagatg gttcagttct
     3361 ccgggttcgc ttctctaagt ctatgtattc aacttaggat actgcacaga atgcagtggg
     3421 tttccccatt cggacatcgc gggatcatag ctttattgcc ggctcccccg cgcttttcgc
     3481 aggcttacac gtccttcgtc gcctatcatc gccaaggcat ccacctgatg cacttattca
     3541 cttgactcta tcatttcaag aacttccttg acttcgttta cctacccgtt gactaagtaa
     3601 gcaaacttga aattcctact ttgataaagc ttactgcttt gttgtgtctt aatcctgcct
     3661 tttgtgtttc aggattaagt cgatacaatc atcacccaaa tactgtgttt gttttctttt
     3721 ctctcgcgag agatttttat cctttgcaaa gaataaaaaa tcaaaacaaa cgctttgtct
     3781 ttgtttgttg atttcggctt tccaatttgt taaagatcga tgcgttcgat attactatct
     3841 actgtgcaag tcaaaacgag ctgattatta tatcagcatt ttgttcttgg tcaagtgtga
     3901 cgtcgccctg aatggattct gttccattct tccgttttga tttgtacagt attggtggag
     3961 gcaaacggga tcgaaccgat gaccccctgc ttgcaaagca ggtgctctac caactgagct
     4021 atgcccccgt tcttggtggg tctgggagga cttgaacctc cgaccccacg cttatcaagc
     4081 gtgtgctcta accagctgag ctacaaaccc ggattctctt cttaagcgaa tcttgccttc
     4141 actcaagctt cttccgcatc tttttcagtt taccgataag tgtgaatgcc taaagcctct
     4201 tctttctcta gaaaggaggt gatccagccg caggttcccc tacggctacc ttgttacgac
     4261 ttcaccccag tcatgaagca taccgtggta agcgggctcc ttgcggttac cctacctact
     4321 tctggtatcc cccactccca tggtgtgacg ggcggtgtgt acaagacccg ggaacgtatt
     4381 caccgcagta tgctgacctg cgattactag cgattccgac ttcatgcact cgagttgcag
     4441 agtgcaatcc ggactacgat cggttttgtg agattggctc cgcctcgcgg cttggctacc
     4501 ctctgtaccg accattgtat gacgtgtgaa gccctggtca taagggccat gaggacttga
     4561 cgtcatcccc accttcctcc ggcttgtcac cggcagtctc attagagtac ccaactgaat
     4621 gatggcaact aatgacaagg gttgcgctcg ttgcgggact taacccaaca tctcacgaca
     4681 cgagctgacg acagccatgc agcacctgtg ttacggctcc cgaaggcact cctccgtctc
     4741 cagaggattc cgtacatgtc aagaccaggt aaggttcttc gcgttgcatc gaattaatcc
     4801 acatcatcca ccgcttgtgc gggtccccgt caattccttt gagttttaat cttgcgaccg
     4861 tactccccag gcggtcaatt tcacgcgtta gctacgctac taagcaatca agttgcccaa
     4921 cagctaattg acatcgttta gggcgtggac taccagggta tctaatcctg tttgctaccc
     4981 acgctttcgg gcatgaacgt cagtgttgtc ccaggaggct gccttcgcca tcggtattcc
     5041 tccacatctc tacgcatttc actgctacac gtggaattct acctccctct gacacactct
     5101 agtcacccag ttcagaacgc agttcccggg ttgagcccgg ggatttcaca tcctgcttaa
     5161 gtaaccgtct gcgcccgctt tacgcccagt aattccgatt aacgctcgca ccctacgtat
     5221 taccgcggct gctggcacgt agttagccgg tgcttattct tcaggtaccg tcatcggccg
     5281 ctggtattag caacagcctt ttcttccctg acaaaagtcc tttacaaccc gaaggccttc
     5341 ttcagacacg cggcatggct ggatcaggct tgcgcccatt gtccaaaatt ccccactgct
     5401 gcctcccgta ggagtctggg ccgtgtctca gtcccagtgt ggcggatcat cctctcagac
     5461 ccgctactga tcgtcgcctt ggtaggcctt taccccacca accagctaat cagatatcgg
     5521 ccgctcgaat agcgcaaggc ccaaaggtcc cctgctttct ctctcaagac gtatgcggta
     5581 ttagctgatc tttcgatcag ttatccccca ctactcggta cgttccgata tgttactcac
     5641 ccgttcgcca ctcgccaccc aagaagcaag cttctctgtg ctgccgtccg acttgcatgt
     5701 gtaaagcatg ccgccagcgt tcaatctgag ccaggatcaa actcttatgt tcaatctcta
     5761 actttttaac ttctggtctg cttcaaagaa accaacagga caatgttcaa aacattatct
     5821 tgtctgtctt tcaaacagtg tgaggctcaa ggcactcaca cttatcggta atctgttttg
     5881 ttaaagagcg tttgaattat aaagtattcc ttccgcctgt caagatatct ctcgatatcc
     5941 ccaacattct gtgct
//
LOCUS       DAXIEB010000110         5939 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000110 DAXIEB010000000
VERSION     DAXIEB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.110"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..357
                     /locus_tag="ACFOA3_001739"
     CDS             <1..357
                     /locus_tag="ACFOA3_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6898708.1"
                     /translation="TTMGERLLTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIK
                     KGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQ
                     QVEQLVQSFVNNSSKK"
     gene            374..648
                     /locus_tag="ACFOA3_001740"
                     /pseudo
     CDS             374..648
                     /locus_tag="ACFOA3_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(735..1868)
                     /locus_tag="ACFOA3_001741"
     CDS             complement(735..1868)
                     /locus_tag="ACFOA3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898709.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGKGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYYIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     IYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQIYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVRRYAEAAARRSGGRRDLSY"
     gene            complement(1988..2482)
                     /locus_tag="ACFOA3_001742"
     CDS             complement(1988..2482)
                     /locus_tag="ACFOA3_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898710.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEVENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQLVKNLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMETFKISVLEDWERLGRPLDWKKWKDQYQSEAFELVKKLNR
                     SKQE"
     gene            2504..2611
                     /locus_tag="ACFOA3_001743"
                     /pseudo
     CDS             2504..2611
                     /locus_tag="ACFOA3_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2736..3071)
                     /locus_tag="ACFOA3_001744"
     CDS             complement(2736..3071)
                     /locus_tag="ACFOA3_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898711.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            complement(3083..3442)
                     /locus_tag="ACFOA3_001745"
     CDS             complement(3083..3442)
                     /locus_tag="ACFOA3_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898712.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKNGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNVNLIQTTN"
     gene            complement(3661..4116)
                     /locus_tag="ACFOA3_001746"
     CDS             complement(3661..4116)
                     /locus_tag="ACFOA3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898713.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIITGGKDNYSTEPYYDE"
     gene            complement(4097..4726)
                     /locus_tag="ACFOA3_001747"
     CDS             complement(4097..4726)
                     /locus_tag="ACFOA3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6898714.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGVTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMHKNERLKIERTIRNIAKNANRKAKGEEHGRRGR"
     gene            complement(4871..5101)
                     /locus_tag="ACFOA3_001748"
     CDS             complement(4871..5101)
                     /locus_tag="ACFOA3_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898715.1"
                     /translation="MEKITQQYAYSELLRLFNQNASDEKIANLAFDFLYAWSKDNSPE
                     SRNIIYDLALIGEPGMELTRNDIKELIDSLVE"
     gene            complement(5089..>5939)
                     /locus_tag="ACFOA3_001749"
     CDS             complement(5089..>5939)
                     /locus_tag="ACFOA3_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272554.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6898716.1"
                     /translation="SDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGIAA
                     GALNPFISAGEALGIGDILYGTGYAIDKAAMRNIAPLPAEGKFAVIGGFGSVAGFEKN
                     TREAVDRWIQENPNAAETVEALANILPFAKAEKLAKLGKAAKPGRAAKLGKAKVSEDF
                     AVATQAKYKQYGDAHSANAQSALAKKLSGLEDAQNSASRTKTLPDGRIRYYEAERFAR
                     TEGRTRGNSFVTEYNPKTGSTRQWTENYDHSGNVTMVHPKSINGQSVTSQHYPPTKQE
                     LDSWKK"
BASE COUNT         1586 a         1348 c         1150 g         1855 t
ORIGIN      
        1 acgactatgg gagaaagatt gcttacttac ggggttaaaa aaatgccatc tgcctatcct
       61 gaatatgaag cttatgaaga taaaagacat attcctgaaa atccatattt tcatgaattt
      121 tactatatta aaaaaggaga aaatccggcg attattactc attggaataa tcgagtaaac
      181 caggctgaag aagataatta tagcactagc gtaggttcct gtattaacgg tttcacggta
      241 cagtattacc cgtttattcg ggaaaagcag cagctcacac agcaggagtt ggtaggttat
      301 caccaacaag tagagcaatt ggtacagagt tttgtaaaca attcaagtaa aaaataattt
      361 aaaggatctt attatgaatg agggtgaagt tgttttaaca ccagaacaaa tccaaacctt
      421 gcgtggttat gcttcccgtg gcgataccta tggcggttgg cgttatttgg ctaatttggg
      481 tgaccgttat gcggataatg ctgctgcaat tgtcggtaag gatgcaaact taaatggttt
      541 gaatttatgg atgaaaaaag ggtggaaaac ctatgggatg atacggtcgg taaaaagatc
      601 cgtttaatgt gtatttccgt tttttggatt gtggttttca atttatagcg aatcggattc
      661 ggcatatacg gcattgcaaa aagcgtctga ctctccaatg ccgtctgaaa accggtttca
      721 gacggcattt gcgttcagta agaaaggtcg cgcctgccgc ccgaacgtct cgccgcagcc
      781 tctgcataac ggcgcacctc tttttccaag tcttccaagt tcaaaggaaa atcaggcagt
      841 ctgtctccct gtttctcttc gcggataatc cgcccaccat ccaaatacca cgtctgttgc
      901 gcatgataga tctgcatatc cgccgttacg ccatccgctt tcaatgctac cgtcgaagat
      961 tgtgcaataa aaagatttcc gtttttcaaa taatattcga aactctggcg ttttttccca
     1021 ttgtcgaaac tccaatagat tttctgcggc agaccgtccg catcatagcc gaccacaaga
     1081 ctgttcgcct tcatcccccg gggcatcaat tcccgcatat tctgataaaa cacataatcg
     1141 cgcgagtccg acgcaatccg gttgctctct tcgcggaagt cccaaacctt ctgctcatca
     1201 ttcgcgacat cccggtattt cgccaaatat acctgggcca tctgataaca cccgaggcaa
     1261 tgctcataaa catcttcccc gattttcccg cgccccgacg catcaaatac cgaaccgtcc
     1321 ggttgccaaa caacccgata ttctcctgtc gtttcataat tttctccgtg agccgttccg
     1381 ccgtacacat ttacagaaaa cggacgatcg tttcgataca gatattcggc attaacaaac
     1441 gcttccggcg agcgttgcga aagcgaaacc gcaaccaaac caccctcgcc gatatagtaa
     1501 tccagccaaa cctctttccc atgttcctgc tccgttacgt gaaaccattt cgccttttct
     1561 ttcaaacgac tgagccggat agcgagcgcg agataatcct tctccgactg caacggaccg
     1621 tcatccacag tttcggcaag actttcctcc gtcctcatcg attccttcac gatgacgacc
     1681 gccctgtcgg catttcggaa caggcgggca agtttcgcca caaaagcatt cggattttca
     1741 ggtatttcag ttgccgtatc gctcaaaaac caacgcggat taatctcata ggcaataccc
     1801 ttccccagcc aaaaggcaaa tacaagtgca aaaaatgaca acaataccgg tttgaatttt
     1861 ttaaacatat ttattttccg tttaacagga tatatcgatt atatcagacg agctttaatt
     1921 gtttgatttt taatctaatc tacggttgct accgtacttt aaaaacccct atgcaggctg
     1981 cggcttgtta ctcttgtttg cttcggttca attttttgac taattcaaat gcttcagatt
     2041 gatattgatc tttccatttt ttccaatcta acggccttcc caagcgttcc caatcttcta
     2101 aaaccgatat tttgaacgtt tccatttccc accaatattc ggcatataaa cgtgattgat
     2161 tgggaatgtc ggcttctgcc caatcggcaa aatgcaaaaa atgagaaata tcgcaaacta
     2221 tattattttc aggaacttct tcggcaaata gattcttgac caactgtaac gcttcggtct
     2281 gatatttttc tgtccatttc tcccattttt caggctttcc cgatatttcc caatcttcca
     2341 atgcggaggc atttaagatt tccaattccc accagcagtc gtaataacgg gaggattggt
     2401 tgggaatttt attttctact tcggaaacaa attgggtaaa actaaaaata tccttaacga
     2461 ttttttcaaa tctattttcc ataaattgct gcctcttcca aattatagtg gattaaattt
     2521 aaatcagaac aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac
     2581 gctgtactgg tttaaattta atccactata cgagccaaca acttcggtaa aatcaagccg
     2641 gcaagcccct gcatcctgtg ctaatcggag aaaatgccgt ctgaaagccg aataccgctt
     2701 cagacggcat ttgccagatt tgaccttgcc ggtgcttacg gacgcttttt aaatataaag
     2761 aaggaaatat cgtctaacgc atcttcggca aaaccttgaa aatccggatt gccttgtttt
     2821 gagcgcaata aagcaatcac tttatcgcaa tcgccgatat tttcatgcat cctggcgaga
     2881 tgtccgaaac aggttaatga caatctggca atatccaagt ctttatgctt cgacaactcc
     2941 gttaatttct cagaaatcca agggatttca tcctcctctg aactgaaggt cataccgagc
     3001 ataccgtcaa taacactctg tttattctca gaataaatca tcttttctac ttgttcctta
     3061 tcagccatca ttttttgtcc atttaattag tcgtttgtat taaattgaca ttatttattt
     3121 tccaatttac tttataacta tcttcataat aatctaattc aaaaaaacct gatatttcaa
     3181 tatccaattc cattattgtt ttaatgcatt tttcaaaata aataatgaaa taagatttca
     3241 cgcatgcgcc aaaaaaaata tagctgctcc aattaaaact atttgtcggg aaaacccacc
     3301 catttttata tatttttgca gattctttct cttcgatatt gaagggacaa ttattccaaa
     3361 aattattaat gttttcttgg atgattttta tatcatcgtc agagcattca atccatcctt
     3421 ttatggaaac atatgatgcc atttaatctc ctaaacctgt tttaacaatg ccgccttttg
     3481 attcaatata tgacttaact tgtgaatgaa caccgtgcct gttccgttca gcaagcacag
     3541 gttggattaa gattcgacaa tctgtttgga ttgacagaaa ggtcgtctga aaaatcgatt
     3601 acggtttcag acgacctttt gttgttgagt tctcaaccca acctatgcct gccattaatg
     3661 ttattcatcg tagtaaggtt ctgttgaata attgtctttg cccccggtaa tgatagtaac
     3721 aattttccct tttgcttccc aagcttgtac tcctatttca tcaaactcat agacatatgt
     3781 cggataagat tcatttgata aataatattt atcaacaccg tatgatttag ggtgatggaa
     3841 aagctgttta aaatcttcaa aattcagacc tattatatta acgcccataa aatatagctc
     3901 ctgataacaa aatatcgaaa taattttgtt tttcttgacg gcaattagta aatttgagtc
     3961 gggagattca taatattctg ttctaaactc atcaggaggt tcatacataa aagtttccag
     4021 tatgtttttg tactgtgtta tatccgcacc aaaacggaat attcctacag aagtaaaagg
     4081 taaaaattcg ggagttttaa cgaccgcgtc gaccatgctc ttctcctttt gcttttcgat
     4141 tggcattttt tgcaatattt ctgattgttc gctcaatctt taatcgttca tttttgtgca
     4201 ttccgggaat aattttattt tttaattcag caattcgtga ttctgctgat atttgacttc
     4261 gaccgccatc tccatgtttt tcattcttgg agcttcctgt tcttttaggc ggacaagaat
     4321 tatgaaccca aaccccttcc gtctccgcct gattgccctt gacgaagtaa gtatgccagt
     4381 cggcaacggt cagattgtag gctttgagcg gctgctgttt gagggtaatg ttttgaactg
     4441 tctgttttgc accgctttcg gaaagcaggg tgtcgccttt tttcagacga cctgcctgta
     4501 tccactttcc ttgactgtaa aacggatgga tgcggttgga aatcagggtt tggctgttgc
     4561 cgatgccgtc tgaaatttca atgtaaacgg tttcttgata cggattgccg tatcgggcgg
     4621 taacaggttt gtatcccgtt accccgcttg cctcgtcctt ggaaaggacg cggtctccgg
     4681 cttgaatatg ggcaatagct ttgtagccgt ctgccgtttt gaccaaggtg ctgtcgtgga
     4741 aggagcaggt gtaggatttt tgaagaactt agttctcaat atcctgtttc aaatgagggg
     4801 aaggtcacct gaaaatcaaa aacggtttca gacaaccttt tgattgtggg attttcagcc
     4861 caacctatgc ttattcaact aaactgtcaa tcaattcttt tatgtcatta cgggtcagtt
     4921 ccatacccgg ttcaccgata agtgctaaat catatattat attacgcgat tctggagaat
     4981 tatcttttga ccaagcatac agaaaatcaa aagcaagatt cgcaattttt tcatccgaag
     5041 cgttttgatt gaacaatctg agcagttcag aatatgcgta ttgttgagtt attttttcca
     5101 tgaatctaat tcctgttttg ttggtggata atgctgagag gttacacttt gtccgttaat
     5161 tgatttagga tgaaccatag ttacgttccc gctatggtca taattttctg tccattgtcg
     5221 ggtactacct gttttaggat tatattctgt aacaaagcta ttacccctcg ttcttccctc
     5281 agttctagca aatcgctcag cttcataata cctgattctc ccatcaggta aagttttcgt
     5341 cctagacgca ctattttgag catcttccaa acctgacaat ttcttagcta atgccgattg
     5401 ggcattggca gaatgcgcat ccccatattg cttgtatttg gcttgtgtgg caacagcaaa
     5461 atcctcgcta acctttgcct tccctagttt tgccgccctc cccggttttg ccgccttccc
     5521 cagtttcgcc aacttctcag ctttggcaaa cggcaggatg ttggccaggg cttcgacggt
     5581 ttcggcggca ttgggatttt cctgtatcca ccggtcaacg gcttcgcgcg tattcttttc
     5641 aaagcccgcc acgctgccaa agccgccgat gacggcgaat ttgccctcgg cgggtaaggg
     5701 ggcgatgttg cgcattgcgg ctttgtctat ggcataaccc gttccgtaca gtatgtcgcc
     5761 tatgcccaag gcttcgcccg cgctgataaa ggggttgagt gctccggcgg caatgccgtt
     5821 gataaactcc atgctgttgc cccagcggtc gagcttggca ttgtgctcga acatttttct
     5881 gttggcttca tcggcgcggt cggagaaatt gctgccgaga ttgttgtagt tgtcggaca
//
LOCUS       DAXIEB010000111         5917 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000111 DAXIEB010000000
VERSION     DAXIEB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.111"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..285
                     /locus_tag="ACFOA3_001750"
     CDS             <1..285
                     /locus_tag="ACFOA3_001750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898717.1"
                     /translation="RRLVLIFVNPLYFHAPAEGRRTVQKIRHSRMPLFCRAYDILSAP
                     VLMPRCGISEFSGYGGGFSFYWERFLQVRRNFLKSIKIYASNCKILISAA"
     gene            425..1648
                     /locus_tag="ACFOA3_001751"
     CDS             425..1648
                     /locus_tag="ACFOA3_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6898718.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            1939..3318
                     /gene="nhaC"
                     /locus_tag="ACFOA3_001752"
     CDS             1939..3318
                     /gene="nhaC"
                     /locus_tag="ACFOA3_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6898719.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(3448..4272)
                     /locus_tag="ACFOA3_001753"
     CDS             complement(3448..4272)
                     /locus_tag="ACFOA3_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6898720.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIKDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(4274..4789)
                     /gene="ybeY"
                     /locus_tag="ACFOA3_001754"
     CDS             complement(4274..4789)
                     /gene="ybeY"
                     /locus_tag="ACFOA3_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6898721.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAVGIPAERDFYRWAWSAL
                     KNKYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            4908..5843
                     /gene="hemC"
                     /locus_tag="ACFOA3_001755"
     CDS             4908..5843
                     /gene="hemC"
                     /locus_tag="ACFOA3_001755"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6898722.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYVRLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESESLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSIVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
BASE COUNT         1232 a         1440 c         1610 g         1635 t
ORIGIN      
        1 cgtcgccttg tcctgatttt tgttaatcca ctatattttc acgcccccgc cgaagggcgg
       61 aggacggtgc aaaaaatacg gcacagccgt atgccccttt tttgtcgggc atacgacatt
      121 ctttccgctc cggttttgat gccacgatgc ggcatttccg aattttccgg atacggcggc
      181 ggattttcat tttattggga acggtttttg caagtccgcc ggaatttttt aaaatctatt
      241 aaaatctatg caagcaactg taaaatatta atttctgctg cttgaatttc agatcggcgc
      301 attgcctgca tccgataaag tttgcaaaat gttcaaatat cagtatgatt tgcattgccg
      361 ttaagaaatg tcaatttcta ttttcttgaa acgggtaata ttccgacacc acgaaaggca
      421 aatcatgtct gcgcaatcac aaaacaatca tacgtcccca ttggtcgtct tgaccacgct
      481 gttcttcatg atgggtttta ttacctgcat gaacgacatc cttatccctc atttgaaaga
      541 aattttcgac ctgtcttacg ttcaggcgat gctgatccaa ttctgtttct ttactgccta
      601 tgcggtgatg tccatcccga tgggggcttt tgtcggcaaa gtcggctaca aaaacggcgt
      661 tatcggcggc tttctgctga cggcggtcgg atgcctgctg ttttatcctg ctgcgggcag
      721 ccattcttac gcggtatttt tgggcgcgtt gtttattttg gcttccggcg taacgctgct
      781 tcaggtcgcc ggtaatcctt atgttaccct gctggcgaaa cccggcaagg aatcggcaac
      841 actgacgctg gttcaggcgt ttaacgcttt gggtacgacc attgcgccgc aaatcggcgc
      901 gttcctgatt ttggcggacg caacccaaac cgtcagcaag gcggaacaga tttcttccgt
      961 acagattccc tatttgggac tggcggggct gctgattatc cttgccgttt tcgtgaaaat
     1021 gatccggctg cccgacgcgc gcaaaattgc cgccgaggaa agcgcgcaca accacgacgg
     1081 caaaaccggc gtatggcaat acaaacatct cgtgttcggt acggcaggca ttttctgcta
     1141 tgtcggcgcg gaggtgtcta tcggttcgtt gatggtcaac gtattgggtt atctgaaagg
     1201 gctggatcat gcttctgccg cgcattacct gtcgttctat tggggcggcg cgatggtcgg
     1261 acgtttcctc ggttcggcgg tgatggcgaa attcgcgccc aaccgttatt tggcgtttaa
     1321 cgcatcggct gcggtcgtac tgcttgccgt cgcgatggcg acgggtagcg gcaatgcgga
     1381 tgtggcgatg tggtcgctgc ttgccatcgg ttttttcaac tcgattatgt tcccgacgat
     1441 tttctctttg gcaaccaaag gattgggaaa atttaccaac gcggcttccg gtgtactgtg
     1501 taccgcgatt gtcggcggtg cggtcgttcc tgtcgtgcag ggctgggtgg cagatactta
     1561 caccctgatg tcttcgtttg tcgtttccgt catctgttat ctgtatatcg tgttttttgc
     1621 ggtgtacgga tatagggcgg acaaataatc tttttcttga gaaatgtcgt ctgaacatct
     1681 ttcagacggc atttttgcgt accggtgttt gcggcgtgtg tgccgaggtt ttaatacttc
     1741 aatccataaa agtcttatat gtcaacaaac aaaaaaataa aaaattatat ttcaaaaaaa
     1801 ttaatttaaa ttgagaaaat tgccgttttg tttctgtccg gcttttgtaa aacgctaaaa
     1861 tgccgtctga aaacgtcggg cggattcggt atggtgtgtt agaatccgtt aactttatat
     1921 caaatcgggc aaagaatcat gttcgctttc aaatccttac tcgatatgcc gcgcggtgag
     1981 gcacttgccg tcgtcgtcgc tctgattgcc gcgatgggct ataccatcat ttcattggag
     2041 tggctgccgc atatgtccat tattgccgcc atcgtcgtgc tgattttgta cggcttggcg
     2101 cgcggtttga aatacaacga tatgcagcag ggcatgatag gcgcgttgaa tcagggtatg
     2161 ggcgcgattt acctgttttt cttcatcggg ctgatggtca gcgcgctgat gatgagcggc
     2221 gcgattccga cgctgatgta ttacggtttc ggactgattt ccccgactta tttttatttt
     2281 tccgccttcg cgctgtgttc cgtcatcggc gtgtccatcg gcagcagcct gaccacctgc
     2341 gccactgtcg gcgttgcctt tatggggatg gcggcggcgt ttcatgccga tatggcgatg
     2401 acggcgggcg cgattgtttc cggtgcgttt ttcggcgaca aaatgtcccc gctttccgac
     2461 accacgggca tttccgcgtc catcgtcggc atcgacttgt ttgagcacat caaaaatatg
     2521 atgtacacca ccatccccgc gtggctcatt agtgcggcac tgatgctttg gcttttgccg
     2581 aatgtcgccg cgcaggattt gaacagcgtc gaatccttcc gcagccagct tgaagccacg
     2641 ggattggtgc acggctattc gctgattccg tttgcactgt tggtcgtttt ggcattgatg
     2701 cgcgtcaatg ccgtggtcgc catgctcttt accgttattg ccgccgttgc cgtaacgtat
     2761 ctgcacagta cgcccgatct gcgtcagctc ggcgcgtggt tttacggcgg ttacaaactc
     2821 gaaggcgaag cgtttaaaga tgttgtcaaa ctgatttcgc gcggcggttt ggaaagtatg
     2881 tttttcacgc aaaccatcgt gattctcggg atgagtttgg gcgggctgct gtttgcgctg
     2941 ggcgcgattc cttccctgct ggatgccgtc cgcagctttt tgacgaatgc cggacgcgcg
     3001 acgttcagcg ttgccatgac ttcggttgga gtgaacttcc tgattggcga acaatatttg
     3061 agcatcctgc tttcgggaga aacgttcaaa cccgtttacg acaggctcgg actacattcc
     3121 cgcaacctct cgcgtacgct cgaagacgcg ggtacggtca tcaacccgct tgttccgtgg
     3181 agcgtgtgcg gcgtatttat cagccatgcg ctgggtgtgc cggtctggga atatctgccc
     3241 tatgcctttt tctgctattt gagtttggct ttaaccctgt tattcggctg gacggggctg
     3301 actttgagca aaaaataagc ggataagcga aatgccgtct gaacctgttt tccggtttca
     3361 gacggcattt ttatgtttgg cggatggggc ggattgaaac agaaaacgcc cgtaccgtca
     3421 tcctaaactg tgcagaaacg gcggtgctta cttcacgcgg gtcgccatca gcgtatgcag
     3481 gcggcggttg tcggcgcgtg cgacggtgaa ctgcaaaccg ccgataagga ctttttcgcc
     3541 gcgcacgggc agatgtccca actcttgaat gaccaggccg ccaatggtgt cggcttcttc
     3601 gctgctgtat tccgtaccga agaaggtgtt gatgtctttg atttcggtag ccgcgtggat
     3661 gcgccagcgt tctgaagaaa cggcgtggat attgtcggcg ctatcgtctt cgtcaaattc
     3721 gtcttcgatt tcgccgacga tttgctcgat gatgtcttca aaggtgacca aaccggatgt
     3781 gccgccgtat tcgtcgatga caatcgccat gtggttgcgc tgttcgcgga actcttttaa
     3841 aagtgcggtc aaagatttgc cttcgggcac gaaaacggca gggcgcaaga cggatttcag
     3901 gtggaactgc tcggggttga acatatattt gagcaggtct ttggcgtgca aaatgcccaa
     3961 aacttcgtct ttgtcttcgc cgatgacggg gaagcgcgaa tgggcggtat cgatgacgta
     4021 ggcggtgatg cgctcgatgc tgtcgttttc ttttaaaacg ttcatacggc tgcgcgtaat
     4081 catggcatcg cgcacttcca aatcggaaaa atcgaggact ttttccaatc ttaaaagcgt
     4141 atccgcatca aaaacttcct gctcgtgcgc ctgccgaagc aggtttaata cgtcttcggc
     4201 ggaatcgggt tcgcgggcga gtcgggcaat caggcgttca aaaaaatttg ttttcggttg
     4261 tgcgccgtcc attttaatat tcgtcctctt ggtaggggtt ggggaagcct gccgccagca
     4321 tcaggcggat ttcttcggct tccattattt cggcttcatc gtcttcgata tggtcgtagc
     4381 ccatcaggtg taaagtaccg tgtatggtca ggtgggcaaa atgctgttcg ggtgttttgc
     4441 cttgttcggc ggcttctttc aaaaccactt gcgggcagat aatcaaatcg ccgtacagtt
     4501 tttccgaaac ttggcagggc aggatttcgc cttcgttgag cgcgaaactc aatacattgg
     4561 tggcgtaatc tttgccgcgg tagtcgcggt tgtaggcgcg ggcttcttct tcgtccaaaa
     4621 gaatcaggct gatgtcggcg cggcggtatt tgtttttcaa ggcagaccac gcccagcggt
     4681 aaaaatcgcg ttcggctggg atgccgacgg cggaagaggc gttttcaaag ttcaaatgga
     4741 aacgttgccg ctgcaacgtt aagaaagggt attttttggt gcgtttcatt gtggcgggtt
     4801 tcgtgttttg tgggtgtaaa tataacatag acctgacggt gccgtctgaa gaaacgttca
     4861 aaatatgata gacttcacgc cgtttccatt ctttgaacgc attgaacatg aacccgaaaa
     4921 aactcgtcat cgccagccgc gaaagcctgc ttgctatgtg gcaggcaaag catatccaag
     4981 gccgtctgaa agctctgtat cccgattgcg aagtcgagat tttgggcatg accacgcgcg
     5041 gcgatcagat tttggacaaa actttgtcaa aagtcggcgg taaaggcttg tttgtcaaag
     5101 agctggaaca ggctttatat gacggccggg ccgatttggc agtgcattcg attaaagacg
     5161 taccgatgga tttgcccgaa ggcttcgcgc ttgcggccat cggcgaacgc gccaatccgt
     5221 ttgatgcgtt tgtgtccaac caatacgtgc gtttggaaga aatgcccgaa ggcgcggttg
     5281 tcggtacatc cagcctgcgc cgcgaagccc agttgcgtgc gcgctatccg catttggtga
     5341 tcaaacccct gcgcggcaat gtgcaaaccc gtttgtccaa actcgataac ggcgaatacg
     5401 acgcaattat ccttgccgcc gccggtttgc agcgtctgga attggacgga cgcatccgta
     5461 tgattttgtc ggaatccgag agcctgcctg ccgccggaca aggcgcgttg ggtatcgaaa
     5521 ttgccgcgca ccgcgaagat ttgtacgaag tcttaaagcc gttgaaccac gataccacac
     5581 acgcctgcgt taccgccgaa cgcgccctcg cacgcgcttt gggcggaagc tgccaagtgc
     5641 cgctggccgc atattgcaca gaagaaaacg gcttgctgac cttgcgcgga ctggtcggac
     5701 accccgacgg ttcgattgtg ttgcgggcgg acgcgcaagc ccctgccgaa tatgccgacg
     5761 cgctcggacg cgctgtcgct aagaaattgg cggacgacgg tgcgcgggaa ttgattggag
     5821 cagtattgaa tacggaaaat tgattttatc gaaaatttaa ataaaataat ataaattatt
     5881 gtttttaatc aatttgtttc atcagtttta ctcgcct
//
LOCUS       DAXIEB010000112         5884 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000112 DAXIEB010000000
VERSION     DAXIEB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.112"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..574)
                     /locus_tag="ACFOA3_001756"
     CDS             complement(<1..574)
                     /locus_tag="ACFOA3_001756"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6898723.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="ACFOA3_001757"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="ACFOA3_001757"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6898724.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2452..3120
                     /locus_tag="ACFOA3_001758"
     CDS             2452..3120
                     /locus_tag="ACFOA3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6898725.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     IYDGDVTDVLLRSVVLNDDINDKRINYAFNHIAFGQTQPATDRVVWTFNQNGRFGYTK
                     DQHLTNQSSFSYSDGYIQIVLTDARAVSGADKKFRSAVVLIDSSGRVEVCPRGDRRTM
                     CQYK"
     gene            3144..3767
                     /gene="pilV"
                     /locus_tag="ACFOA3_001759"
     CDS             3144..3767
                     /gene="pilV"
                     /locus_tag="ACFOA3_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6898726.1"
                     /translation="MKNNDCFHLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLSHTLSAVDGDFQ
                     VDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGVAPTLSAGSTFSSNCD
                     ASANGDTLIKVLWVNDSAGDSDIDRTDLETNGNNIVYTYQARVGGRE"
     gene            3764..4753
                     /locus_tag="ACFOA3_001760"
     CDS             3764..4753
                     /locus_tag="ACFOA3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6898727.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHTKGDIIVDPSKQIQP
                     ASAKPGAKQENPLFSLEWANTNTNNNKAKLIPIAESTDIKYPGFTQSGKALVFQYGID
                     DFDASAETVVVSSCSKIAKPGKKIPTLAEAKSALKITDDKQNGNITRQKHEVNAYAVG
                     KISDVEGLFRFQLKDGQWGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDK
                     FNSAQDAVTPAGVEVLLSSGTDTKITASSDNHIYAYRIDATIRGGNICANRTL"
     gene            4732..5331
                     /locus_tag="ACFOA3_001761"
     CDS             4732..5331
                     /locus_tag="ACFOA3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6898728.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNHGNEEAFDNIVVQGNPTVEAVKRSCTAKSTGLCIDNKGMEYKKGTQSVSKPPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5336..5833
                     /locus_tag="ACFOA3_001762"
     CDS             5336..5833
                     /locus_tag="ACFOA3_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6898729.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVSINNAFKQIILKNPGKSNQDLEAELRKFASDYRMNPKIAEKYSVSGEFVDAPKSRA
                     SRALKSRAYRLVGVPKAGTGYTLSVWVNSVGDGYKCRDAASAQAHLETLSADAGCEAF
                     SNRKK"
BASE COUNT         1585 a         1300 c         1526 g         1473 t
ORIGIN      
        1 cgaagcgttt ggcgacttcg tcccagttga cgatttccca gaaacctttc aggtagttgg
       61 ggcggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggcgtagcgg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc catgcccaac cggagccgaa agtaccggca gcgcaggcat
      241 tgaacgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccggaagg tttgccttga cctttggacg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgcgc cgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag ctcggcagcg cacatatttt acctgttttg
      661 agggatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacac agctatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg ctgcctccac attcgatgga ggcggaacaa tccgttttgg gcggtttgat
      841 gctggaaaat ccggcttggg acaggattgc cgatgtggtt tccggtgaag acttctaccg
      901 ccacgaacac cgcctgattt tccgatccat tgctaaatta atcaatgaga gccgtcctgc
      961 cgatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 atttgaatat ctgattacgc tggcgcaaaa taccccgtct gccgccaata tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccattat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcctacaatc cgcaaggcag ggacgcgggg cagcttttgg acgaggcgga
     1201 aaacaaagtg tttcaaatcg ccgaaagcac ggccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacagggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcaacccggc gacctgatta ttgttgccgg tcgtccatct atgggtaaga ccgccttttc
     1441 tatcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggtgcgcaac tggtcatgcg tatgctcggc tcggtcggac ggttggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgcctga acgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtttga ctgcgctcga
     1681 actgcgcgcc cgcgcccgcc gtctcgcccg tcaatttaac aataagctgg gattgatcgt
     1741 catcgactac ctgcaactga tggcgggatc cggccgttcc gacaaccgag cttcagagct
     1801 gggcgaaatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ctgagccgca cggtcgaatc gcgtaccgac aaacgcccca tgatgtccga
     1921 ccttcgcgag tccggcgcaa tcgagcagga tgccgacctg attatgttca tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaagat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaaat atagtggatt
     2281 aacaaaaatc aggacaaggc aacgaagccg cagaccgtac aaatagtacg gaaccgattc
     2341 acttggtgct tgagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgccgt
     2401 actggtttga atttaatcca ctatacatta ccagttaacg ttctattgct tatgtgtaca
     2461 cgaaaacaac aaggtttcac gctaacagag ctgctcatcg tgatggtcat tgcagccatt
     2521 atggcgatga tagccctccc caatatgagc caatggattg catcccgccg cattgccagt
     2581 cacgcggagc ggattgccaa ccttttgcgt ttctccaggg gcgaagccgt ccggctcaat
     2641 ctccctgtct atatctgtcc tgttcaagtt aaaaaagacg gtacgcccaa caataaatgt
     2701 gactccggca agaaggggca gggaatgttg gctttcggcg acaaaaacgg caataagata
     2761 tatgacggtg atgtgacgga tgttcttctc cgcagtgtgg tattgaatga tgatatcaat
     2821 gataagcgga ttaattatgc cttcaaccat atcgcttttg gtcagactca gccggccacc
     2881 gaccgtgtag tttggacatt caatcaaaac gggaggttcg gttatacgaa agaccagcat
     2941 cttacaaacc aatccagctt ttcttattcc gacggttata tccaaatcgt gctgacagat
     3001 gcgagagcag tttcaggtgc cgataagaaa ttccgttcgg cggtggtttt gattgacagc
     3061 agcggcaggg tcgaagtttg ccctaggggt gatcggcgta ctatgtgcca gtataaataa
     3121 cagtttcagt tttaaaaatg aatatgaaga ataatgattg cttccacctg aaaaatcccc
     3181 agtccggtat ggcactgata gaagtcttgg ttgctatgct cgttctgacc atcggtattt
     3241 tggcactatt gtccgtacag ttgcggacag tcgcttccgt cagggaggcg gagacacaaa
     3301 ccatcgtcag ccaaatcacg caaaacctga tggaaggaat gttgatgaat ccgaccattg
     3361 attcggacag caacaagaaa aactataatc tttacatggg aaaccataca ctatcgcata
     3421 cactatcagc tgtggatggc gattttcagg ttgatgccat aaaaactaag acgcagttgg
     3481 cagaggcaca attgaagaga tttagttatg agctgaaaaa tgccttgccg gatgcggcag
     3541 ccatccatta cgccgtctgc aaggattcgt cgggtgttgc gccgacattg tccgccggca
     3601 gtactttttc ttcaaattgc gatgctagtg caaatgggga tactttgatt aaagtattgt
     3661 gggtaaatga ttcggcaggg gattcggata tcgaccgtac ggatcttgag acgaacggca
     3721 acaatatcgt atatacctat caggcaaggg tcggaggtcg ggaatgagac gtaaaatgct
     3781 aaacgtacca aaaggcagtt atgatggtat gaagggtttt accattattg aatttttggt
     3841 tgcgggcatg ctcagtatga ttgtcctgat ggcggtcgga tcgagttact tcacatcccg
     3901 gaaattaaat gatgcggcaa acgagcgtct ttccgcgcaa caggatttgc ggaatgcggc
     3961 aacattgatt gtccgcgatg cgagaatggc gggcggcttc ggttgtttca atatgtccga
     4021 gcatactaaa ggggatatta ttgttgatcc aagtaagcaa attcaacctg cctctgcaaa
     4081 acccggtgcc aaacaagaaa atcccctttt ttctttagag tgggctaata ctaatactaa
     4141 taataataaa gctaaattga ttcctattgc tgaatccaca gatattaaat atccgggttt
     4201 tacccaatcc ggaaaggcat tggttttcca atacggtatt gatgattttg atgcgagtgc
     4261 tgagactgtt gtagtcagca gctgttccaa aatagcaaaa ccgggtaaga aaatacctac
     4321 cttggcagaa gcaaagagtg cattaaagat tacggatgat aaacaaaatg gaaatatcac
     4381 ccgtcagaaa catgaggtca acgcctatgc ggttggcaag atttctgatg tggagggttt
     4441 attccgtttt caattgaagg atgggcagtg gggtaatcct cagttgttgg ttaaaaagat
     4501 taataaaatg gatatacggt atatttatgt ttccggctgt cctgaagatg acgatgccgg
     4561 caaagaggaa acattcagat atacggataa attcaacagc gcccaagatg ctgttacgcc
     4621 cgccggggtg gaggttttat tgagtagcgg tactgatacc aagattaccg cttcttcaga
     4681 caatcatatt tatgcttacc gtatcgatgc gacaatacgc gggggaaata tatgcgcaaa
     4741 cagaacgctt tgacgggaat cccgacttct gacggacaga gggggttcgc actgtttatc
     4801 gtgctgatgg tgatgatcgt cgtggcattt ttggttgtaa ctgccgcgca gtcttacaat
     4861 accgagcaga ggatcagtgc caacgaatca gacaggaaat tggctttgtc tttggccgag
     4921 gcggctttgc gggaaggcga atttcaggtt ttggatttgg aatatgatac ggacagtaag
     4981 gttacattta gcgaaaactg tgaaaaaggt ctgtgtaccg cagtgaatgt gcggacaaat
     5041 acaaatcatg gtaatgaaga ggcttttgac aatatcgtgg tgcaaggcaa tcccaccgtt
     5101 gaggcggtga agcgttcttg cactgcaaaa tctacaggcc tgtgcattga caataaaggg
     5161 atggaatata agaaaggcac gcaaagtgtc agcaaaccgc cgcgctatat tatcgaatat
     5221 ttgggcgtga agaacggaga aaatgtttat cgggttactg ccaaggcttg gggtaagaat
     5281 gccaataccg tggtcgtcct tcaatcttat gtaagcaata atgatgagta ataaaatgga
     5341 acaaaaaggg tttacattga ttgagatgat gatagtcgtc gcgatactcg gcatcatcag
     5401 cgtcattgcc ataccttctt atcagagtta tattgaaaaa ggctatcagt cccagcttta
     5461 tacggagatg gtcagtatca acaatgcttt caaacagatt attttgaaaa atcccgggaa
     5521 aagcaatcag gatcttgaag cggaactgag gaagtttgcg tcggattatc ggatgaatcc
     5581 taaaattgcc gaaaaatata gtgtttcggg ggaatttgtc gatgcgccaa aatcaagggc
     5641 aagcagggcg ttaaaatcaa gggcatacag gttggtcggc gttccgaagg cggggacggg
     5701 ttataccttg tcggtatggg tgaacagcgt gggcgacgga tacaaatgcc gtgatgccgc
     5761 ttctgcccaa gcccatttgg agaccttgtc cgcagatgcc ggctgtgaag ccttctctaa
     5821 tcgtaaaaaa taaggttgtt ttgccgatgc cgtctgaaaa tcaatgttca gacggtattt
     5881 ttat
//
LOCUS       DAXIEB010000113         5841 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000113 DAXIEB010000000
VERSION     DAXIEB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.113"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            44..457
                     /locus_tag="ACFOA3_001763"
     CDS             44..457
                     /locus_tag="ACFOA3_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6898730.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            503..1132
                     /gene="nth"
                     /locus_tag="ACFOA3_001764"
     CDS             503..1132
                     /gene="nth"
                     /locus_tag="ACFOA3_001764"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6898731.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDDVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            1270..2769
                     /locus_tag="ACFOA3_001765"
     CDS             1270..2769
                     /locus_tag="ACFOA3_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6898732.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAENDSDPIADNDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEDLPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTSGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALITKILPSSP
                     AERAGLQAGDIIRSLNGEEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2970..4490
                     /locus_tag="ACFOA3_001766"
     CDS             2970..4490
                     /locus_tag="ACFOA3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6898733.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRHGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAAAGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRTNA"
     gene            4547..5632
                     /gene="nagZ"
                     /locus_tag="ACFOA3_001767"
     CDS             4547..5632
                     /gene="nagZ"
                     /locus_tag="ACFOA3_001767"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6898734.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDSESASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVTRLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELETADLIPFRGMSREGMAA
                     VMPAHVVYPQVDAKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     LSFEAGCDIILVCNRPDLVDELRDGFTIPDNPDLAGRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5713..5789
                     /locus_tag="ACFOA3_001768"
     tRNA            5713..5789
                     /locus_tag="ACFOA3_001768"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5747..5749,aa:Pro,seq:cgg)
BASE COUNT         1363 a         1761 c         1450 g         1267 t
ORIGIN      
        1 gtacaatccg tcgccgattt ttgcctgttt gagactgttt gcaatgatac cctcctacac
       61 ctacgccgcc ctcgcctttt ccgccttcac ttccgccacc ctcctgcccg gcacatccga
      121 agccgccttt gccctgttcg tccaccgctt tcccgaacac gcatacggcg cgttgctgtg
      181 cgccggcctt gccaacggat tgggcagtat ggtttcctat tggatggggc gtttgctgcc
      241 ctcccgaaaa atgccgtctg aaaaaacact gaatctgatg cggcgtttcg gtatttggct
      301 gcttgcgttt gcctggctgc ccgtcgtcgg cgacgcactg ccgcttgccg ccggctggct
      361 gcggttgaat ccgtggacaa gcgggctgat gctggttatc ggcaaaacgg cgcgttacgc
      421 cttcattctg tggggaatgc aatattacgc cgcctgaacc gtgcgttata atgccgaccg
      481 cacaaaccga ctgcacaaaa aaatgaacag acaaatccgc caagaaatct tcgagcgttt
      541 ccgcgccgcc aacccccatc cgaccaccga gctgaatttc aactcccctt tcgagctttt
      601 aatcgccgtt ttactgtcgg cgcaggcgac cgatgtcggc gtaaacaagg cgacggcgaa
      661 gctgtttccg attgccgata cgccgcaagc gatgctggat ttgggcttgg acgacgtgat
      721 ggaatacacg aaaaccatcg ggttgtataa aaccaagtcc aaacacatca tgcagacctg
      781 ccgcatcctg ctggaaaaat acaacggcga agtgccggaa gaccgcgagg ctttggaatc
      841 attgcctggc gtggggcgca aaacggcaaa cgtggtattg aacacagcgt tcggacaccc
      901 cgttatggcg gtcgatacgc atattttccg cgtgtccaac cgaaccaaaa tcgctcccgg
      961 gaaagatgtg cgcgaagtcg aagacaaact gatgcgcttc attcctaaag agttcctgat
     1021 ggacgcgcac cactggctga ttttgcacgg acgctacacc tgcaaggcac tcaaaccgca
     1081 atgccaaacc tgcatcatca acgatttgtg cgaatatccc gccaaatcct gaccgccgcc
     1141 gcccttcaaa atgaaatgct tgaaaattcg gacggacgtt gtaaaatttc actccatgcc
     1201 gtctgaaacc gacaacgtgc gtttcagacg gcattgaagt caatatcggt acgaaacaaa
     1261 ggacgaaacg tgttcaaaaa ataccaatac ctcgctttgg cagcactgtg tgccgcctcg
     1321 ctggcaggct gcgacaaggc aggcagcttt ttcggtgcgg acaaaaaaga agcatccttc
     1381 gtagaacgca tcgaacacac caaagacgac ggcagcgtca gtatgctgct gcccgacttt
     1441 gcccaactgg ttcaaagtga aggtccggca gtcgtcaata ttcaggcagc ccccgccccg
     1501 cgcacccaaa acggcagcgg caatgccgaa aacgattccg acccgattgc cgacaacgac
     1561 ccattctacg aatttttcaa acgcctcgtc ccgaatatgc ccgaaatccc ccaagaagaa
     1621 gcagatgacg gtggattgaa cttcggctcc ggcttcatca tcagcaaaga cggctacatc
     1681 ctgaccaata cgcacgtcgt taccggcatg ggcagtatca aagtcctgct caacgacaag
     1741 cgcgaatata ccgccaaact catcggctcg gatgtccaat ccgacgtggc gcttttaaaa
     1801 atagacgcaa cggaagacct ccctgtcgtc aaaatcggca atcccaaaga tttgaaaccc
     1861 ggcgaatggg tagccgccat cggcgcaccc ttcggcttcg acaacagcgt tacctccggc
     1921 attgtgtccg ccaaaggcag aagcctgccc aacgaaagct acaccccctt catccaaacc
     1981 gatgtcgcca tcaatccggg caactccggc ggtccgctgt tcaacttaaa aggacaggtc
     2041 gtcggcatca actcgcaaat atacagccgc agcggcggat ttatgggcat ttcctttgcc
     2101 atcccgattg acgttgccat gaatgttgcc gaacagctga aaaacaccgg caaagtccaa
     2161 cgcgggcaac tgggcgtgat tattcaagaa gtatcctacg gtttggcaca gtccttcggt
     2221 ctggataaag ccagcggcgc attgattacc aaaatccttc ccagcagccc cgcagaacgt
     2281 gcaggcttgc aggcaggcga catcatacgc agtctcaacg gagaagaaat acgttcttcc
     2341 ggagaccttc ccgttatggt cggtgccatt acaccaggaa aagaagtcag cctcggcgta
     2401 tggcgcaaag gcgaagaaat tacaatcaaa gtcaagctgg gcaacgctgc cgagcatacc
     2461 ggcgcatcat ccaaaacaga tgaagccccc tacaccgaac agcaatccgg tacgttctcg
     2521 gttgaatccg caggcattac ccttcagaca cataccgaca gcagcggcgg acacctcgtc
     2581 gtcgtacggg tttccgacgc ggcagaacgc gcaggcttga ggcgcggcga cgaaattctt
     2641 gccgtcgggc aagtccccgt caatgacgaa gacggtttcc gcaaagctat ggacaaggcg
     2701 ggcaaaaacg tccccctgct ggtcatgcgc cgtggcaaca cgctgtttat cgcattaaac
     2761 ctgcaataac acacataatt taaccaaaat attttttaaa tgataaaatg cccatcaata
     2821 tcgattaatg ggcattttaa aaaatcggtt gtttgatttt aaacaaatat tttttacgac
     2881 aaaaatattt ccgcccattt tgccaacaaa acccaaaccg gaggcggaca gataccctcc
     2941 catccggacg cttaacaccc ccataaacca tgcagctgat cgactattca cattcatttt
     3001 tctcggttgt gccacccttt ttggcactgg cacttgccgt cattactcgc cgcgtactgc
     3061 tgtctttagg catcggtatt ctggtcggcg ttgccttttt ggtcggcggc aaccccgtcg
     3121 acggtctgac acacctgaaa gacatggtcg tcggcttggc ttgggcagac ggcgattggt
     3181 cgctgggcaa accaaaaatc ttggttttcc tgatactttt gggtattttt acttccctgc
     3241 tgacctactc cggcagcaat caggcgtttg ccgactgggc aaaacggcac attaaaaacc
     3301 ggcacggcgc gaaaatgctg accgcctgcc tcgtgtttgt aacctttatc gacgactatt
     3361 tccacagtct cgccgtcggt gcgattgccc gccccgttac cgacaagttt aaagtttccc
     3421 gcgccaaact cgcctacatc ctcgactcca ctgccgcgcc catgtgcgtg ctgatgcccg
     3481 tttcaagctg gggcgcgtcg attatcgcca cgcttgccgg actgctcgtt acctacaaaa
     3541 tcaccgaata cacgccgatg gggacgtttg tcgccatgag cctgatgaac tattacgcac
     3601 tgtttgccct gattatggtg ttcgtcgtcg catggttctc cttcgacatc ggctcgatgg
     3661 cacgtttcga acaagccgcg ttgaacgaag cccacgatga aactgccgtt tcagacgcta
     3721 ccaaaggtcg tgtttacgca ctgattattc ccgttttggc cttaatcgcc tcaacggttt
     3781 ccgccatgat ctacaccggc gcgcaggcaa gcgaaacctt cagcattttg ggggcatttg
     3841 aaaacacgga cgtaaacact tcgctggtat tcggcggcac ttgcggcgtc cttgccgtcg
     3901 ttctctgcac gctcggcacg attaaaaccg ccgactatcc caaagccgtt tggcagggtg
     3961 cgaaatctat gttcggcgca atcgccattt taatcctcgc ttggctcatc agtacggttg
     4021 tcggcgaaat gcacaccggc gattacctct ccacactggt tgcgggcaac atccatcccg
     4081 gcttcctgcc cgtcatcctc ttcctgctcg ccagcgtgat ggcgtttgcc acaggcacaa
     4141 gctgggggac gttcggcatt atgctgccga ttgccgccgc catggcggtc aaagtcgaac
     4201 ccgcgctgat tatcccgtgt atgtccgcag taatggcggg ggcggtatgc ggcgaccact
     4261 gctcgcccat ttccgacacg accatcctgt cgtccacagg cgcgcgctgc aaccacatcg
     4321 accacgttac ctcgcaactg ccttatgccc tgacggttgc agccgcagcc gcagccggtt
     4381 acctcgcatt gggtctgaca aaatccgcgc tgttgggctt tggcacgacc ggcattgtat
     4441 tggcggtgct gatttttctg ttgaaagaca aaaaacgcac caacgcctga cccctttccc
     4501 tgtgcagacg gcatatgccg tctgaagctt tttggagcaa accgaaatga ctgttcccca
     4561 tattccgcgc ggccccgtca tggcagacat tgccgccttc cgcctgaccg gagaagaaaa
     4621 acaacgcctg ctcgaccccg caatcggcgg catcatcctc ttccgccgca acttccaaaa
     4681 catcgaacag ctcaaaacac tcaccgccga aatcaaagcc ctccgcacac ccgaactcat
     4741 catcgccgtc gatcacgaag gcggcagggt tcaacggttt atcgaaggat ttacccgctt
     4801 gcccgcaatg aacgtcttag gacaaatttg ggacagcgaa agcgcatctg ccgccgaaac
     4861 cgcagcccgg cagataggct gggttttggc aaccgagctt tccgcctgcg gcatcgactt
     4921 gtccttcact cccgtcttgg atttggactg gggaaactgc cccgtcatcg gcaaccgcag
     4981 tttccaccgt aaccccgaag ccgtaacccg ccttgccctc gcccttcaaa aaggtttgga
     5041 aaaaggcggc atgaaatcat gcggcaaaca ttttcccgga cacggatttg tcgaaggaga
     5101 cagccatctg gtcttgccgg aagacaggcg cagcctgtcc gaactcgaaa ccgccgacct
     5161 tatccccttc cgcggcatga gccgggaagg tatggcagcg gtcatgcctg cccacgtcgt
     5221 ttatccgcaa gtggatgcca agcccgcagg gttttccgaa atctggctca aacaaatttt
     5281 gcgccgcgat atcggattca aaggcgtaat tttctctgac gatttgacca tggaaggcgc
     5341 gtgcggggct ggcggtatca aagaacgcgc ccgactgtcg tttgaagcag gttgcgacat
     5401 catattggtc tgcaaccgtc ccgacttggt cgatgaattg cgcgacggtt tcaccattcc
     5461 cgacaacccg gatttggccg gacgttggca atacatggcc aacacgctcg gcagtgccgc
     5521 cgcgcaagcc gtgatgcaga caacggattt ccaagcggcg caagcctttg ttgccggtct
     5581 ggcctcgccg caagacacgg cgggcggcgt gaaagtcgga gaggcctttt aaatcctccc
     5641 gacacgcggc aaaaaggcaa aacggtatag gcatgccgct ctttttacgt tataatcccg
     5701 ttccaattca ttcggaatgt agcgcagccc ggtagcgcac ttcgttcggg acgaaggggt
     5761 cggaggttcg aatcctctca ttccgaccaa atccaaaccg cctcggtatc cattgccggg
     5821 gcggtttttc cttttggcgg c
//
LOCUS       DAXIEB010000114         5642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000114 DAXIEB010000000
VERSION     DAXIEB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.114"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(254..2050)
                     /locus_tag="ACFOA3_001769"
     CDS             complement(254..2050)
                     /locus_tag="ACFOA3_001769"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6898735.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2106..2447
                     /locus_tag="ACFOA3_001770"
     CDS             2106..2447
                     /locus_tag="ACFOA3_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898736.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFIPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2508..3761
                     /locus_tag="ACFOA3_001771"
     CDS             2508..3761
                     /locus_tag="ACFOA3_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6898737.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHKLTAGMNF
                     LAIVGLLYLTGFAVLFLLNLSGLLA"
     gene            3916..5427
                     /gene="lysS"
                     /locus_tag="ACFOA3_001772"
     CDS             3916..5427
                     /gene="lysS"
                     /locus_tag="ACFOA3_001772"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6898738.1"
                     /translation="MSEQNHPQIEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQAVPVKVAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASCTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5428..>5642)
                     /locus_tag="ACFOA3_001773"
     CDS             complement(5428..>5642)
                     /locus_tag="ACFOA3_001773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898739.1"
                     /translation="LGGIWGENSRKPVLGFRLSGGKGILQRSLFVLSAARRLVLIFVN
                     SLSILPYKMPSESNRVQTALPFQLFL"
BASE COUNT         1232 a         1446 c         1622 g         1342 t
ORIGIN      
        1 cggaatatag cggattaaca aaaaccagta cggcgttgtc gcgccttagc tcaaagagaa
       61 cgattctctc aggtgctgaa gcaccgagtg aatcggttcc gtactattcg tactgtctgc
      121 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttgcttgcg gcaagatgaa
      181 aacggtgcgg gatgttttgg gaaaccaatg ccgtctgaag ggctttcaga cggcattttt
      241 tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt gatcagccac gcttttgccg
      301 cgccttcggt cagcggctcg aggcggcggc ggacttcggc gtggtagcgg ttgacccagt
      361 cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg gggcagaggg
      421 tcagggtttc aaaacagagg aagctgccga attcggtttc ttgaggggcg gcgacggctt
      481 ggttggcggc aaggttttca atgcggatgc cccattttcc cgggcggtag agtccgggtt
      541 cgatggaggt aaccatgcct tttttcatgg cggtttcggg cgtggcgggg gcggcgaagg
      601 cgatgcgctg cgggccttcg tggacgttga ggaaatagcc tacgccgtgt ccggtgccgt
      661 ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca aatcgcatca atcagcggcg
      721 aggggatgtt ttcggggaac acggcttcgg caagcgcgat atgggctttg agaacgaggg
      781 tgttgtcgcg tttttgttcg gcggtgggtg tgccgacagg gacgacgcgg gtgatgtcgg
      841 tcgtgccgcc tttgtattgc gcgccggagt cgatgagcaa aagcccgttg ccgctgatgg
      901 tgctgtggct ttcgggcgtt gcgctgtaat gcggcagtgc gccgttggcg ttgaagcctg
      961 cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac gctgcggtgg cgataaagca
     1021 tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct tcaaactcgg
     1081 cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg cgggcgatgt
     1141 cggcttcgga tttgcaggat ttgaacagcg tggacgggtt gattccctcg ataaggcgca
     1201 cgctttcggg caggcgcaca agcgtgctga cggcggtttt gttcggttcg ataagcaacg
     1261 cgccgccgat ttgcgcgagt ttgtcggcaa cttgggcgta aggctcgacc gtgatgcctg
     1321 cggtttgcag cgcggcggcg gcttcggcat tcagacggca tcggtcggta aacaggacgg
     1381 cgttgtcttt gccaatcaat agataggaca cgaaaacggg attgaaaggc acgtcgctgc
     1441 cgcgcaggtt ggtcagccag gcgatgtcgt cgagcgagga aaccaagtgg taatccgcgc
     1501 ctttttccgc catcacggcg cgcacgcggg cgagtttttc ggcggcggtt tcggagacgt
     1561 aggcggggtc gtggacgaac acggtttcgg cggggatagc gggacggctt gtccacactt
     1621 ggtcaagcag atcgtccggg tgttcgatgc ggatgttttt ggcggtcagg gattgcgcca
     1681 aagtgcgttt gccggtgagc gagaccatat cggaagggat gccgacggcg gcgttttcgg
     1741 gcaggtttgc cgcgagccat tcgttatacg gcggcacttg cccgcttttt tgcagttcga
     1801 tgccgctgcc cgcaagctgc ttggcggctt gttcccaata gcggctgtcc acccatacgc
     1861 ccgcttcatc ggtggtcagg acaaacgtgc cgaccgagcc ggtaaagccc gacaattcgc
     1921 gccgcgcctg ccaatgttcg ggcaggtatt cggacaggtg ggggtcggcg gaagggatga
     1981 cgagtgcatc caagccttgc gccttcatgg cttcgcgcaa tgcggacagg taattcgata
     2041 cggtattcat aacagttcct ccaatcgggt tttgcggctt cagacggcat ggacggaaat
     2101 ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc tgcaaaacgg
     2161 acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt gtttgttgta
     2221 aaattttagg cataaaatgt cgttttattc ctttttttgg aagtaatatg gaaaatatag
     2281 tcaagtttga gcatgaagtt ctaatagtgt gtgttggcag gtatttggat tacgttacaa
     2341 tacgtgtttt catcaaaatt tccctaccgg gcggtatcct gtttttgaag acggtaggat
     2401 ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa tagatagggc tgtgtccgaa
     2461 tatgcggctc tgtgtgttga aacacagtta aaaatacgga agactttatg tccgaacaac
     2521 atatttcgac ttggaaaagt aaaatcaacg cattgggtcc ggggatcatg atggcttcgg
     2581 cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt tacggctggc
     2641 agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc cgcttcagcg
     2701 cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag aaaagccgtg
     2761 tttatttgtg ggttttcctg attttgtgca tcatttccgc cacgattaac gctggcgcgg
     2821 tcgccatcgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg tttgatgccg
     2881 gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc ggacgttacc
     2941 gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc acgcttgccg
     3001 ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc cgattttatc gagccgacac
     3061 cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg cccgcgccga
     3121 ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc aatccttccg
     3181 aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg gttttggctt
     3241 tggttttcct tgcactgggc gcgtttgtgc aatacggcaa cggcgaagca gtgcagatgg
     3301 cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc ggcggctggt
     3361 cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta cggcacgacg attaccgtcg
     3421 tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga aaagacaaaa
     3481 cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt ttggcggtga
     3541 ttttttggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt gccgcctttg
     3601 tgtccgcccc cgtgtttgcc tggctcaact accgcctggt caaaggagac aaacggcaca
     3661 agcttaccgc cggtatgaat tttcttgcca ttgtcggctt gctctacctg accggatttg
     3721 ccgttttgtt cctgttgaac cttagcggac ttttggcata ggtttcaaaa tctgaatgcc
     3781 gtctgtaata ttttcagacg gcattgccct ttcctagaaa aaaacaccga catcgcgtaa
     3841 aatatgcaca aattttgtga ttcaatcagg tcgtctgaaa cagattgcgc ctgatttatt
     3901 ttttcggaaa ataccatgag cgaacaaaac catccgcaaa tcgagccgca gttggacgaa
     3961 aaccaaatta tcgccctgcg ccgtgaaaaa ctgcacaaca tccgccaaca gcgcaacgcc
     4021 taccctaacg atttcaaacg cgacagcttt gccgccgatt tgcacgccca atacggcgaa
     4081 atcggcaaag aagaactcga cccgcaggcc gttcccgtca aagtggccgg ccgcatgatg
     4141 ctgaagcgtc aaatgggcaa agccagcttc gccaccattc aagacgttac cggtcaaatc
     4201 cagctttatc tgaacaacaa aggcgtgagt caggaagttt tggacgattt caaccattgg
     4261 gatttgggcg atatcgtcgg cgcggaaggt actttgttca aaaccaacca cggcgaattg
     4321 accgtgcgcg tgtccgacat ccgcctgttg tccaaatcct tgcgtccgct gccggacaaa
     4381 cacaaaggct tgagcgatca ggaaaccaaa taccgccaac gctatgtcga tttgattgcc
     4441 aacgaagaat cgcgcaatac ctttatcaaa cgcagccaaa tcatccaatc cgtgcgtaat
     4501 tttatggtgg gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc gattcccggc
     4561 ggcgcgacgg caaaaccctt cgttactcat cacaatgcct tagatattcc gctttacctg
     4621 cgtatcgcgc ctgagctgta tttgaaacgc ctggttgtcg gcggcttgga acgcgtgttt
     4681 gaaatcaacc gcagcttccg caacgaaggc atgtccgtgc gccacaaccc cgaatttacg
     4741 atgattgaat tttacgaagc cttctccgac tacgaacgta tgatgcagat ggcagaagac
     4801 atcatcagaa acgcatcgtg cacagtaaac ggtacggcaa acatcactta caacggcaaa
     4861 gaagtcgatt tggaaagccc gtttgagcgt ctgaccatcc tcgaagccat caaaaaatac
     4921 aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa agaaatcgtc
     4981 aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact cgcgctgttt
     5041 gaaggttgcg ccgagggcaa gctgtggaac ccgactttca tcatcgatta cccggtcgaa
     5101 gtttccccat tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg tttcgaattg
     5161 ttcgttgtcg gccgcgaact ggcaaacggc tattccgagt tgaacgaccc cgaagaccaa
     5221 gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga agcgatgcac
     5281 tacgatgccg actacatccg cgcgatggag ttcggtctgc cgccgacagg cggttgcggc
     5341 atcggtatcg accgcttggt aatgctgctg accgattcgc aaaccatccg cgatgtgatt
     5401 ctgtttccgc agatgcgccc cgagtaatca taaaaacagt tgaaatggca atgccgtctg
     5461 aacccgattg gattcagacg gcattttgta tggcaggatt gatagtgaat taacaaaaat
     5521 caggacaagg cggcgagccg cagacagtac aaatagagac ctttgcaaaa ttcctttccc
     5581 tcccgacagc cgaaacccca acacaggttt tcggctgttt tcgccccaaa taccgcctaa
     5641 tt
//
LOCUS       DAXIEB010000115         5543 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000115 DAXIEB010000000
VERSION     DAXIEB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.115"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>5543
                     /locus_tag="ACFOA3_001774"
     CDS             <1..>5543
                     /locus_tag="ACFOA3_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6898740.1"
                     /translation="SAGNVAVNAEGKLVNTGMIAATGENHAVSLHARNVHNSGTVASQ
                     DDANIHSQTLDNSGTVLSSGQLTVRNLGRLKNHNNGTIQAARLDMSTGGLDNTGNITQ
                     TGSQALDLVSAGKFDNSGKIGVSDVPQTGLNPNPSVIPQIPSTATGSGSSTVSASKPG
                     SNNPVSPTAPAKNYAGGRIQTTGAFDNAGSINAGGQIDIAAQNGLGNSGSLNAAKLRV
                     SGDSFNNTVKGKLQAHDLAVNTQTAKNSGHLLTLTGKIDNRELHNAGEIAANNLTLIH
                     SGRLSNDKKGNIRAAHLQLDTAGLHNAGNILADSGTVTAKNNLRNTGKVSVARLNTEG
                     QTLDNTHGHIEAETVNIQSQQLTNQSGHITATEQLTINSRNVDNQNGKLLSANQAQLA
                     VSDGLYNQHGEIATNRQLSIHDKNQNTLALNNADGTIQSAGNVSLQAKSLANNGTLTA
                     GNKLDIALTDDFVVERDLTAGKQLNLSIKGRLKNTHTLQAGHTLKLNAGNIDNQVTGK
                     IIGGEQTDITSEQHVDNRGLINSDGLTHIGAGQTLTNTGTGKIYGNHIALDAQILLNR
                     EETTEGSTKAGAIAARKHLDIGAKEIHNQEGALLSSEGIFAVGNRLDEQHHAAGMADT
                     FVNGSASLEVQGDALMSVRNMQNINNHFKTETYLAKAEKQVRDYTVLGQNTYYQAVKD
                     GLFDNSHGKKDQTTATFHLKNGSRIEANQWHVRDYHIETYKERIIENRPAHITVGGNL
                     TASGQNWLNKDSRIVVGGRIITDDLNQKEITNQSTTGKGRTDAVGTQWDSVTKKGWYS
                     GRKRQRRTERNHTPYHDTQLFTHDFDTPVSVIQQNAASLSFQPAASAIKLIDGVSTAA
                     VNGQRIHTGNVVSLNNATVTLPNSSLYTTHPDNKGWLVETDPQFADYRRWLGSDYMLQ
                     QLKLDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRRLDGYRSDEEQFKALMDNGLTAAK
                     TFALTPGIALSAEQVARLTSDIVWMENQTVTLSDGSTQTVLVPKVYALARKGDLNTSG
                     GLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQARNINSNGNIQADQIGLKAEKSINID
                     GGQVQAGRLLTAQAQNINLNGTTQTSGNERNGNTAIDRMAGINVVGSHTEQVDNRTSD
                     GILSLHAGNDINLNAATVSNQVKDGTTQITAGNNLNLGTIRTEHREAYGTLDDENHRH
                     VRQSTEVGSSIRTQNGALLRAGNDLKIRQGELEAEEGKTVLAAGRDVTISEGRQITEL
                     DTSVSGKSKGILSSTKTHDRYRFSHDEAVGSNIGGGKMIVAAGQDINVRGSNLISDKG
                     IVLKAGHDIDISTAHNRYTGNEYHESKKSGVMGTGGLGFTIGNRKTTDDTDRTNIVHT
                     GSIIGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTVTAKSIDVEFANNRYATDYAHTQE
                     QKGLTVALNVPVVQAAQNFIQAAQNVGKSKNKRVNAMAAANAAWQSYQAAQQMQQFAP
                     SSSAGQGQNNNQSPSISVSITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSGE
                     QSNINITGSDVIGHAGTALIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGIR
                     FGITAGGNIGKGKEQGGNTTHRHTHVGSTTGKTTIRSGGDTTLKGAQLIGKGIQADTR
                     NLHIESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQSKVKADHASVTEQSGIYAGE
                     DGYQIKVRDNTDLKGGIITSTRSAESKGKNRFQTATLTHRDIQNHSRYEGKSFGIGGS
                     FDLNGGWDGTVTDKQGRPTDRISLAAGYGSDSDSQSSITKSGINTRNIHITDEAGQLA
                     RTGRTA"
BASE COUNT         1787 a         1441 c         1252 g         1063 t
ORIGIN      
        1 tcagccggca atgtggcagt gaatgctgag ggtaaactgg tcaacacagg catgattgca
       61 gcgacgggag aaaatcatgc ggtttcactt catgcccgca atgttcataa tagcggtacg
      121 gttgcctcac aggatgatgc caatattcac agccagacgc tggacaattc aggtacggtc
      181 ttatcctcag gtcaattaac tgtccgtaat ttaggccgtc tgaaaaacca taacaacggt
      241 acgatccagg ctgcacgctt agatatgtca acaggtggtt tggataacac aggtaatatt
      301 actcaaacag gttcacaagc attggatttg gtatctgccg gcaaattcga taacagtggc
      361 aagattggcg tgagcgacgt tccacagacc ggtttgaatc ccaatccgtc agtcatacca
      421 cagattccga gtactgcaac aggttcaggc agcagcactg tctcggcatc taagcctggt
      481 tcaaacaatc ccgtttcacc tacagcacct gcaaaaaact acgccggagg acgcattcaa
      541 acaacaggag catttgacaa tgcaggatca atcaatgcag gtgggcaaat tgacattgcc
      601 gcccaaaacg gtttgggaaa ttcgggtagt ctgaatgcgg ctaaactacg agtatcaggc
      661 gattcattta acaatacggt aaaaggcaaa ctccaggcac acgatctggc tgttaacact
      721 caaactgcta aaaacagcgg tcacttatta actctaaccg gcaagattga caaccgtgaa
      781 ctgcataatg ccggagaaat tgccgccaac aatctgacac tcattcattc gggccgcttg
      841 agcaatgata aaaaaggcaa tattcgagct gcacatttac agcttgatac cgccggttta
      901 cataatgcag gtaacattct tgccgatagt ggaaccgtta ccgccaagaa taatcttcgc
      961 aatacaggaa aagtttctgt tgcacgactg aataccgaag gtcagactct agataatacg
     1021 cacggacata tagaggctga aacggttaac atccaaagtc agcaactgac taaccaaagc
     1081 ggccatatta ctgctaccga acaactgact atcaatagtc gaaatgtaga caaccaaaac
     1141 ggcaaactcc tatctgcaaa ccaagcacaa ttagctgttt cagacggcct atacaaccaa
     1201 catggtgaaa ttgccaccaa ccggcagttg tctattcacg ataaaaatca aaacactttg
     1261 gcgttaaaca atgcggatgg cacgattcaa tctgccggta atgtatcgct acaagccaaa
     1321 tcactcgcca acaatggcac attaacagcc ggtaacaaac tggatattgc tttgacggac
     1381 gatttcgtcg tagagcgcga cctcactgca ggcaaacaat taaatctaag cataaaaggc
     1441 cgtctgaaaa atacccatac cctacaagca ggccatacgc tcaaactcaa tgccggcaat
     1501 atagataacc aagttacagg taaaattatt ggcggagaac aaacggacat cacatccgaa
     1561 cagcatgttg acaacagggg cttgatcaac agcgacggtt tgacccacat cggtgcaggt
     1621 caaaccctga ccaacaccgg gacaggcaaa atctatggca accatattgc cctggacgcg
     1681 caaatactgc ttaaccgaga agaaacgacg gaaggcagta ccaaagcggg ggcaatagct
     1741 gcaaggaaac atttggatat tggagcgaaa gagattcata accaagaagg tgccctacta
     1801 tccagcgaag gtatttttgc cgtaggtaat cgactggatg aacaacatca tgcggcaggc
     1861 atggctgata cctttgttaa tggcagtgcc agtttggaag tacaaggtga tgcattgatg
     1921 tccgttagga atatgcagaa tatcaataat cattttaaaa cagagacata cttagccaaa
     1981 gcggaaaagc aagtccgcga ctacaccgta ttggggcaaa atacctatta tcaggcagta
     2041 aaagacggcc tattcgacaa ctcgcacgga aaaaaagacc aaacaactgc taccttccat
     2101 ttaaaaaatg gttctcgtat tgaggccaac caatggcatg tccgagacta ccacatcgag
     2161 acttataaag aacgcatcat cgaaaaccgg ccggcacaca ttactgtggg cggtaatttg
     2221 actgcctcag gtcaaaattg gctgaacaaa gacagccgga ttgtagtagg cgggcgtatt
     2281 atcactgatg atttaaacca gaaagaaatt accaatcaaa gtacaacagg caaaggtcgc
     2341 acagatgctg tcggcacaca gtgggattca gttacaaaaa aaggatggta cagcggtaga
     2401 aaaagacaac gccgtactga aagaaaccat actccttacc atgataccca actatttacc
     2461 cacgacttcg acacgcctgt atccgtcatc caacagaatg ccgcctccct ttcctttcaa
     2521 cccgctgcat ctgcaatcaa actgattgac ggagtatcca cagccgccgt caatggtcag
     2581 cgcatccata ccggtaatgt ggtctcgtta aataacgcta ctgttactct gcctaacagc
     2641 agcctctata ccacccatcc tgacaataaa ggctggttgg ttgaaaccga tcctcaattt
     2701 gcagactacc gccgctggtt gggcagcgac tacatgttgc aacaactgaa attggacacc
     2761 aatcatctac acaaacggct tggcgacggc tactacgaac aaaaacttgt taatgaacaa
     2821 atccatcagt taacaggcta ccgccgactc gacggctaca ggagtgatga agaacaattc
     2881 aaagctctga tggacaacgg ccttactgct gccaaaacat tcgctctcac cccaggtatc
     2941 gccttgagtg cagagcaagt tgcccgctta acttcagata tcgtttggat ggaaaatcaa
     3001 accgtcaccc tgtctgacgg ttcgactcaa accgtactgg ttcctaaagt ctatgccctg
     3061 gcgcgcaaag gtgatctcaa tacctccggt ggcctgatta gtgccgaaca agtcttactt
     3121 aaactgcaaa acggcaacct gactaacagc ggtaccattg cggggcgaca ggccgtactc
     3181 atccaggcac ggaatattaa cagcaacggc aacattcaag ccgaccaaat cggcttaaaa
     3241 gctgaaaaaa gtatcaatat cgacggcggg caggtacaag caggcagact gctgactgcc
     3301 caagcgcaaa atatcaacct taacggtaca acccaaactt ccggtaatga acgtaacggc
     3361 aataccgcca tcgatcgtat ggccggcatt aacgtggtcg gaagccatac tgaacaagta
     3421 gataacagaa cttcagacgg catcctatcc ctgcatgccg gcaacgatat caacctcaat
     3481 gcggccaccg tctctaacca agttaaagac ggcactaccc aaattaccgc cggcaataat
     3541 ctcaacctcg gcaccatccg taccgaacat cgcgaagcct atggtacatt agatgacgag
     3601 aaccatcgcc atgtccgcca aagtaccgaa gtcggcagca gtatccgcac gcaaaacggc
     3661 gcactgctta gagccggtaa cgacttaaaa atccgccaag gcgaactaga ggccgaagaa
     3721 ggcaaaaccg tccttgccgc gggacgtgat gtcactatca gcgaaggacg ccaaataacc
     3781 gaactggata cctcggtaag cggaaaaagc aaaggcatcc tttccagtac caaaacacac
     3841 gaccgctacc gcttcagtca tgatgaagca gtcggcagca acatcggcgg cggcaaaatg
     3901 attgttgcag ccgggcagga tatcaatgta cgcggcagca accttatttc tgataagggc
     3961 attgttttaa aagcaggaca cgacatcgat atttctactg cccataatcg ctataccggc
     4021 aatgaatacc acgagagcaa aaaatcaggc gtcatgggta ctggcggatt gggctttact
     4081 atcggtaacc ggaaaactac cgatgacact gatcgtacca atattgtcca tacaggcagc
     4141 attataggca gcctgaatgg agacaccgtt acagttgcag gaaaccgcta ccgacaaacc
     4201 ggcagtaccg tctccagccc cgaggggcgc aataccgtca cagccaaaag catagatgta
     4261 gagttcgcaa acaaccggta tgccactgac tacgcccata cccaggaaca aaaaggcctt
     4321 accgtcgccc tcaatgtccc ggttgtccaa gctgcacaaa acttcataca agcagcccaa
     4381 aatgtgggca aaagtaaaaa taaacgcgtt aatgccatgg ctgcagccaa tgctgcatgg
     4441 cagagttatc aagcagccca acaaatgcaa caatttgctc caagcagcag tgcgggacaa
     4501 ggtcaaaaca acaatcaaag ccccagtatc agtgtgtcca ttacctacgg cgaacagaaa
     4561 agtcgtaacg agcaaaaaag acattacacc gaagcggcag caagtcaaat tatcggcaaa
     4621 gggcaaacca cacttgcggc aacaggaagt ggggagcagt ccaatatcaa tattacaggt
     4681 tccgatgtca tcggccatgc aggtactgcc ctcattgccg acaaccatat cagactccaa
     4741 tctgccaaac aggacggcag cgagcaaagc aaaaacaaaa gcagtggttg gaatgcaggc
     4801 gtagccgtca aaataggcaa cggcatcagg tttggaatta ccgccggagg aaatatcggt
     4861 aaaggtaaag agcaaggggg aaatactacc caccgccaca cccatgtcgg cagcacaacc
     4921 ggtaaaacta ccattcgaag cggcggggat accaccctca aaggtgcgca gctcatcggc
     4981 aaaggcatac aggcagatac gcgcaacctg catatagaaa gtgttcaaga tactgaaacc
     5041 tatcaaagca aacaacaaaa cggcaatgtc caagttactg tcggttacgg attcagtgca
     5101 agcggcagtt accgccaaag caaagtcaaa gcagaccatg cttcggtaac cgagcaaagc
     5161 ggtatttatg ccggagaaga cggctatcaa atcaaagtca gagacaacac agacctcaag
     5221 ggcggtatca tcacctccac ccgaagtgcc gaaagcaaag ggaaaaaccg ctttcagacg
     5281 gccaccctca cccatcgcga cattcaaaac cacagccgct acgaaggcaa aagcttcggc
     5341 ataggcggca gtttcgacct gaacggtggc tgggacggca cggttaccga caaacaaggc
     5401 aggcctaccg acaggataag cctggcagcc ggctacggca gcgacagcga cagtcaaagc
     5461 agcatcacga aaagcggcat caacacccgc aacatacaca tcaccgacga agcgggacaa
     5521 cttgcccgaa caggcaggac tgc
//
LOCUS       DAXIEB010000116         5517 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000116 DAXIEB010000000
VERSION     DAXIEB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.116"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            255..647
                     /locus_tag="ACFOA3_001775"
                     /pseudo
     CDS             255..647
                     /locus_tag="ACFOA3_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            714..1076
                     /locus_tag="ACFOA3_001776"
                     /pseudo
     CDS             714..1076
                     /locus_tag="ACFOA3_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1160..1522
                     /locus_tag="ACFOA3_001777"
                     /pseudo
     CDS             1160..1522
                     /locus_tag="ACFOA3_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1739..2059
                     /locus_tag="ACFOA3_001778"
                     /pseudo
     CDS             1739..2059
                     /locus_tag="ACFOA3_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            2196..2618
                     /locus_tag="ACFOA3_001779"
                     /pseudo
     CDS             2196..2618
                     /locus_tag="ACFOA3_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            2752..3169
                     /locus_tag="ACFOA3_001780"
                     /pseudo
     CDS             2752..3169
                     /locus_tag="ACFOA3_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247143.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            3279..3676
                     /locus_tag="ACFOA3_001781"
                     /pseudo
     CDS             3279..3676
                     /locus_tag="ACFOA3_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            3906..4337
                     /locus_tag="ACFOA3_001782"
     CDS             3906..4337
                     /locus_tag="ACFOA3_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6898741.1"
                     /translation="MPTRLDSRLRGNDEAILLAEGQKSAVTEYYLNHGIWPGGNSDAG
                     VASSSTIKGKYVQKVEVKNGVVTATMLSSGVNKEIKGKKLSLWAKRQNGSVKWFCGLP
                     VERTAPTTTNDDVKADTAANGKQIDTKHLPSTASTRKSTPN"
     gene            5296..>5517
                     /gene="pilE"
                     /locus_tag="ACFOA3_001783"
     CDS             5296..>5517
                     /gene="pilE"
                     /locus_tag="ACFOA3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HFC6898742.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGIWPANNTSAGVA"
BASE COUNT         1696 a         1573 c         1187 g         1061 t
ORIGIN      
        1 tttccgataa attcctaaaa cttaaaattt catcattccc acaaggacaa aaaaccaaaa
       61 acagaaacct aaaattcgtc attcccgcaa aagtgggaat ctagttcgtt gagtttcagt
      121 tatttagaat aaattttgaa acttaaatcc cgtcattccc acgaaagtgg gaatccagaa
      181 cgcaaaatct caagaaaccg ctttcccgat aagtttccgt accgacaagg ctggattccc
      241 gcctgcgcgg gaataacgaa tccattcttt tggctgaaag tcaaaaatca gccgttaccg
      301 agtattacct gaatcacggc atatggcccg gcgacaacag ttctgccggc gtggcaacct
      361 cctccgacat caaaggcaaa tatgttgaga aagttgaagt cgcaaaaggc gtcattaccg
      421 ccacaatgct ttcaaccggc gtaaacaacg aaatcaaagg caaaaaactc tccctgtggg
      481 ccaagcgtca aaacggttcg gtaaaatggt tctgcggaca gccggctcag cgcgccggca
      541 ccgacagcga caccgacgtc aaagccgaca ccgccgccaa cggcaagcag atcgacacca
      601 agcacctgcc gtcaacagca ccgacaagaa aatcgacacc aaactagaga aaccttttta
      661 cccgataagt ttccgtaccg acaaggctgg attcccgcct gcgcgggaat gacgaagcta
      721 tccttttggc cgaaggtcaa aaatcagccg tcaccgagta ttacctgaat cacggcatat
      781 ggcccgccaa caacaattct gccggcgtgg caacctccgc caacatcaaa ggcaaatatg
      841 ttgagaaagt tgaagtcaaa aacggcgtcg ttaccgccac aatgctttca accggcgtaa
      901 acaaagaaat caaaggcaaa aaactctccc tgtgggccaa gcgtcaagac ggttcggtaa
      961 aatggttctg cggacagccg gttacgcgcg acgccgtcgc caaagacgac gccgttaccg
     1021 ccgccgccag cgacaagcag atcgacacca aactaaagaa accgttttcc cgataagttt
     1081 ccgtaccgac aaggctggat tcccgcctgc gcgggaatga cggcggccgg gcaccgattt
     1141 caaatacttt aatacttcca aaaaatcagc cgtcaccgag tattacctga atcacggcga
     1201 atggcccggc aacaacactt ctgccggcgt ggcaacctcc gccgacatca aaggcaaata
     1261 tgttgagaaa gttgaagtca aaaacggcgt cgttaccgcc acaatgcttt caaccggcgt
     1321 aaacaaagaa atccaaggca aaaaactctc cctgtgggcc aagcgtcaag ccagttcggt
     1381 aaaatggttc tgcggacagc cggttcagcg cgacgccgtc gccaccgacg ccgccatcac
     1441 cgccgccagc gacgccagcg acgccaccgc caacatcgac accaagcacc tgccgtcaac
     1501 ctgccgcgac gcagcatctg ccgtttgcac aaaaacacca cgcgccgatt tcaaacactt
     1561 tcaaaaaatc agccgttacc gagtattacc tgaatcacgg caaatggcag aaaaacttcg
     1621 tcattcccgc gcaggcggga atctaggttt gtcggtgcgg aaacttatcg ggtaaaaagg
     1681 tttctccagt cctgaatcct agattcccac tttcgtggga atgacgggat ttaatgatgc
     1741 cgccggcatg gcatcctcct ccgacatcaa aggcaaatat gttgagaaag ttgaagtcgc
     1801 aaaaggcgtc attaccgcca caatgctttc aagcggcgta aacaacgaaa tcaaaggcaa
     1861 aaaactctcc ctgtgggcca agcgtcaaga cggttcggta aaatggttct gcggacagcc
     1921 ggttgagcgc aacaccgccg ccgacgacga cgtcgccgac gacagcggca acgacaaaat
     1981 cgacaccaaa ctaaagaaac cgttttaccc gataagtttc cgtaccgaca gacctggatt
     2041 cccgcctgcg cgggaatgac gaatccatcc atacggaaat ctacatcccg tcattcccac
     2101 gaaagtggga atctaggacg taaaatctaa agaaaccgtt ttcccgataa atttccgtac
     2161 cgacagacct agattcccgc ctgcgcggga atgacgaagc tatccttttg gctgaaggtc
     2221 aaaaatcagc cgttaccgag tattacctga atcacggcga atggcccggc aacaacagtt
     2281 ctgccggcgt ggcatcttct tcaacaatca aaggcaaata tgttaaggaa gttgaagtca
     2341 aaaacggcgt cgttaccgcc acaatggctt caagcaacgt aaacaacgaa atcaaaggca
     2401 aaaaactctc cctgtgggcc aagcgtcaag acggttcggt aaaatggttc tgcggacagc
     2461 cggttgagcg cgacgccaac gccaaagacg acgttgccgc cgccaccgcc aacggcaacg
     2521 gcaagatcga caccaagcac ctgccgtcaa cctgccgcga cgcttcatct gccgtttgca
     2581 tagaaacacc acctacggct ttctataaaa atacctaaac cgtcattccc acgaacctac
     2641 atcccgtcat tcccacgaaa gtggggatcc agaacgcaaa atctaaagaa accgttttcc
     2701 cgataagttt ccgtgccgac gaggctggat tcccgcctgc gcgggaatga cgaatccatc
     2761 ctttttggcc gaaggtcaaa aatcagccgt cacagagtat tacctgaatc acggcgaatg
     2821 gcccggcaac aacagttctg ccggcgtggc aacctccgcc tccgacatca aaggcaaata
     2881 tgttcaaagc gttgaagtcg caaaaggcgt cattaccgcc acaatgcttt caaccggcgt
     2941 aaacaaagaa atccaaggca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt
     3001 aaaatggttc tgcggacagc cggttacgcg cgacgccacc aacgccgacg acgttaccgc
     3061 cgacggcaac gacaaaatcg acaccaagca cctgccgtca acctgccgcg atgattcatc
     3121 tgtcgtttgc atagaaacac cacctacggc tttctataaa aatacctaaa ccgtcattcc
     3181 cacgaaagtg ggaatccaga acgcaaaatc tcaagaaacc gttttcccga taagtttccg
     3241 taccgacggg gctggattcc cgcctgcgag ggaatgacga agcgatcctt ttggccgaag
     3301 gtcaaaatca gccgttaccg agtattacct gaatcacggc gaatggcccg gcaacaacac
     3361 ttctgccggc gtggcaacct ccaccgacat taaaggcaaa tatgttcaaa gcgttgaagt
     3421 caaaaacggc gtcgttaccg ccacaatggc ttcaagcaac gtaaacaacg aaatcaaagg
     3481 caaaaaactc tccctgtggg ccaagcgtca aaacggttcg gtaaaatggt tctgcggact
     3541 gccggttacg cgcaacaacg ccaaagccaa cgccgccaac gccgacgacg tcgccaagac
     3601 cggtaccgac agcgaaaaaa tcaacaccaa gcacctgccg tcaacagcac cgacaagaaa
     3661 atcgacacca aactagagaa acctttttac ccgataagtt tccgtaccga cgaggctgga
     3721 ttcccgcctg cgcgggaatg acggcggagc ggtttctgtt gctcccgata aattcctaaa
     3781 acttaaaatt tcatcattcc cacaaggaca gaaaaccaaa aacagcaacc taaaattcgt
     3841 cattcccgca aaagcgggaa tccagaacgc aaaatctcaa gaaaccgttt tcccgataag
     3901 tttccgtgcc gacgaggctg gattcccgcc tgcgcgggaa tgacgaagct atccttttgg
     3961 ctgaaggtca aaaatcagcc gtcaccgagt attacctgaa tcacggcata tggcccggcg
     4021 gcaacagtga tgccggcgtg gcatcttctt caacaatcaa aggcaaatat gttcagaaag
     4081 ttgaagtcaa aaacggcgtc gttaccgcca caatgctttc aagcggcgta aacaaagaaa
     4141 tcaaaggcaa aaaactctcc ctgtgggcca agcgtcaaaa cggttcggta aaatggttct
     4201 gcggactgcc ggttgagcgc accgccccca ccaccaccaa cgacgacgtc aaagccgaca
     4261 ccgccgccaa cggcaagcag atcgacacca aacacctgcc gtcaacagca tcgacaagaa
     4321 aatcgacacc aaactagaga aaccttttta cccgataagt ttccgtaccg acaaggctgg
     4381 attcccgcct gcgcgggaat gacggcggag cggtttctgt tgctcccgat aaattcctaa
     4441 aacttaaatt ttcatcattc ctacaagaac agaaaaccaa aaacagaaac ctaaaattcg
     4501 tcattcccgc aaaagcggga atctagttcg ttgagtttca gttatttaga ataaattttg
     4561 aaactttaat cccgtcattc ccgcgaaagt gggaatccag aacgcaaaat ctaaagaaac
     4621 cgttttcccg ataaatttcc gtaccgacgg ggctggattc ccgcctgcgc gggaatgacg
     4681 aagctatcca tacggaaatc tacatcccgt cattcccacg aaagtgggaa tccagaacgc
     4741 aaaatctaaa gaaaccgctt tcccgataag tttccgtgcc gacgaggctg gattcccgcc
     4801 tgcgcgggaa tgacggcgga gcggtttctg ttcggacaca ggaacaccac gcgccgattt
     4861 caaacacttt ccaagaaaac ggaaattttt aaaaattaaa tttaaaattt cccacccaac
     4921 ccaccctatg ctaccgcgca aattcaaaaa tctcaaattc cgacccaatc aacacacccg
     4981 atgccccatg ccaatagaaa taccccacac caataaaaaa gtgccgaaaa tcggcactaa
     5041 aactgacaat tttcaacact gctgacaaaa aacggcaacc ccctaattac tcaaaccacg
     5101 ccaaacgaaa gaaaatgtaa aataaaaaca atcatataaa gataaacgca taaattttca
     5161 cctcaaaata caaacttggt accaatcttg ctttataata cacaggtatc gcaacaaaat
     5221 accgatggtc aaatacattg cataatgccg atggcgtaag cttgaagcat ttcctttcca
     5281 attaggagta attttatgaa cacccttcaa aaaggtttta cccttatcga gctgatgatt
     5341 gtgattgcca tcgtcggcat tttggcggca gtcgcccttc ctgcttatca agactacaca
     5401 gcccgcgcgc aagtttccga agccatcctt ttggccgaag gtcaaaaatc agccgtcacc
     5461 gagtattacc tgaatcacgg catatggccc gccaacaaca cttctgccgg cgtggca
//
LOCUS       DAXIEB010000117         5458 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000117 DAXIEB010000000
VERSION     DAXIEB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.117"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(183..3014)
                     /gene="lbpA"
                     /locus_tag="ACFOA3_001784"
                     /gene_synonym="tbpA"
     CDS             complement(183..3014)
                     /gene="lbpA"
                     /locus_tag="ACFOA3_001784"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6898743.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNKPLSAEEEAMVREAQARHENLSAQSYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDNNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3011..5233)
                     /locus_tag="ACFOA3_001785"
     CDS             complement(3011..5233)
                     /locus_tag="ACFOA3_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6898744.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGSSVETTPVNRPAVGAAMRLPRRNIAAFDKNGNEIPNSKQAEEYLPLKEE
                     DILFLDGTPQEQADKLKKKINERYPNVKIYTSDSKDAEYEYVRAGYVYTRFGTDEIEQ
                     NSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDAKRHRAGKAVGID
                     NLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFGNKTLTGELIKNQYVKPSEKQK
                     PLTIYNITADLNGNRFTGSAKVNPDLAKNLAGNERLFFHADADQRLEGGFFGDKGEEL
                     AGRFISNDKSVFGVFAGKQKTEAANAADTKPALTSGKHTKILDSLKISVDEASGENPR
                     PFAISTMPDFGHPDKLLVEGREISLLKDTQTIDLADGRKMTVRACCDFLTYVKLGRMQ
                     TERPAAKPKAQDEEDSDIDNGEEGEDEIVDEDEVSEEDEEEIEEAEEEEAEPEEAEEA
                     EEESPTEEGNGGLGSIPPTQEASKGRDIDLFLKGIRTAEADIPQTGTAHYTGTWEARI
                     SKPIQWDNQADKEAAKAEFDVDFGRKSISGKLTEKNGVEPAFHIENGKIEGNVFYATA
                     HTRESGINLSGNGSTNPQRFKASNLRVEGGFYGPKAAELGGTIFNKDGKFLDITKDTE
                     NEVEADVEVEADVEVEADVEVEADVEVEADVSEQLKPEVKPQFGVVFGAKKDMQEVEK
                     "
BASE COUNT         1178 a         1477 c         1267 g         1536 t
ORIGIN      
        1 agctttttga gtttcagtca tttccgataa attgccttag cattgaatgt ctagattccc
       61 gcctgcgcgg gaatgacggc ggaaatcttg tttatatcga atcaaaaaaa cctgcacctt
      121 aatcagttgg cggtttagtc cgacttttgg ggtgcagatc aagctttcag acggtatttc
      181 ctttaaaact tcatttcgag cgcgaggctg aagttcctgc ccggtgcggc ataccttcca
      241 tagttgctgt cgccgccgtg ccggtttgcc gtgctttccg cagtctggcg caaggattcc
      301 caagtaacgt agcggtagtt gccgatattg tagatagccg ccctcaaggt cagccgtttt
      361 ttcagattca gataggcgga aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc
      421 gaatatcgtt tttgatcgcc tgccagataa gcaagctcgt cagggttttt ccctttggaa
      481 taggtcagca taatgtttgc gccccatttc ccctctggct ggtcgtatcc gaaccccaaa
      541 acataacgcg acggctgtac cgcatccaaa gcatagctgc ggagggacag tcccggccgg
      601 ttggataccg atttcggttt gatacggttg tacgccaatg tggtgtacaa accttcgggc
      661 agtttgccgt acacaccgtt ccagtcgatt tttcccaata tattaacgcc ttggagcgac
      721 atattttggg cattgtaata atcgcgtata tcaatctctg tcaattgtcc tgcctgattc
      781 ggcaatttgg ttttgtgatc ggcaacggca atcatatcgg tatagcggtt gcggaagctg
      841 ctgatttcca aaaagccgaa atcgcccttc cactgcaaac cgatttcgcg gttggctgcc
      901 ttttccgatt tcagggcggg acgctgccag cctttcggat aatcgtgata aatgtctatc
      961 ccgaaaagtt cttggaatga gggcgttctg aagccgctgg aggcgcggta agacacggaa
     1021 aaatgccggt tcggtttgaa cacgatgccg ctgttccacg aacggtcaac ataccgcccg
     1081 ctgcggacga gttcttccga cgtggtgaag tttttccggt catacctgcc gcccaagctg
     1141 aaatcgaaat atttgccgat tgaaaaacgg tcgttcaaag aaatatggat attgctgccg
     1201 ttgatttttc ttggcacgca tttgcgggaa cgcagggttt cgatgtagcc gcagaccgac
     1261 ccttcgacga cttcgggctt acccaaacgg tacttatctt gattgttatc atcgaatccc
     1321 gtggattccg aaatccttgc cgcattgtgg gaaagctgtt cggggcggga gatcgctttg
     1381 gaagcatcgt aaccgaagcc caaagtcaga tggtgtttcg tccatttgtt tttcagcgat
     1441 ttctcaaacg aggcattcaa aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg
     1501 tcgtaggaat acggtttgtc cgccgacgcg cggcaggatt tgtccacagc aggatacacg
     1561 gcgcaattca gcttcagcgt gttgttgtcg gttgccacgc cctgtttgtc aaacgacaac
     1621 accgccttgt ccgcccaatt gtcagaatac gcttcgtttt cataacggta cagcaaaccc
     1681 atacggcggc ggcggtgatg ttcgtcaata aatttggtgc gggaatattt caaacctatg
     1741 cccttgacca aatttgtatc gcccttccac tcttctatat tcggcacaaa atacaagccg
     1801 tcgcggaaat cgtcgccgtc gtacaccccg ctcttgtctc taaacttttc cgcctcgtcc
     1861 gtaccgtaat actgtttttc cgtcatatcg cggatgtcgt aacgctgttt ggtatcctca
     1921 aacacgccgc cgacataatg ccttccgccg aagcggtagc ccagcttggc aagccaagag
     1981 ccgctgcggt aatccatcgg atcgggcaat atcctgccgc cgcccgtgta cgattgggcg
     2041 gacaggtttt cgtggcgcgc ctgcgcctcc cgcaccatcg cttcttcttc agcacttaaa
     2101 ggcttatttt gttcaatacg ttcttttacc cagcggttaa gctggcggtt caaatatatc
     2161 ccgtagcccg ccaattttgc cacgggcttg agtgtattct cgccctctac taagaaaaat
     2221 tgccctgttt tattgctttg aatatcgtgt gtctggcgga acgcgtccaa acggtttatg
     2281 ccgtatgcca ccccgtctgc aatatcgccg tgcgggcgcg tttcccgccc ttgacgttcg
     2341 gttcggatta gcagcccttc ccaaccgtct ttgctgaacc ccgcgccgag cgacttcata
     2401 aattggcggt ttttactgcc gtaggcggtt tttgcctgta ttccccaact cttgccgtct
     2461 gaaatcaggt ctgccgcctc tttggttcgg aaggcgaccg cgccgccgag tgcgccgctg
     2521 ccgtgatcgg acgaaccggc acctttgtcg atttccaccg tgctgatgtt ttcatattcg
     2581 atttcgttga ttgcaccgct gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg
     2641 gtaaacgcct gtatttgggc aacgccgtca accgaaaccg ccacacggtt tttatccacg
     2701 ccgcgtatcg agtagccgcc gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga
     2761 tcgtagcgcg tcaggtcgcg gataccgagt acctgttcct tgttcaacgt ttccgacgtt
     2821 ttgacgattt tgcccaaacc ggtcgcctct ttcgatcgcc gtcccacttt ggcggcacgg
     2881 acggtaacct ctttcaagga ttgggtctgc gccgcatctg gtgccgccgt ttcagggttt
     2941 gccgcatatg acgggaaggc ggcggcaaca gccaaggcag tcagggtaag ctgaaaaccg
     3001 tgtttcttat tcatttttcc acctcctgca tatctttctt cgcaccgaat accacgccga
     3061 attggggttt aacttcaggt tttaactgtt cgctaacatc agcttcaact tcaacatcag
     3121 cttcaacttc aacatcagct tcaacttcaa catcagcttc aacttcaaca tcagcttcaa
     3181 cttcattttc agtatcttta gttatatcaa gaaatttccc atccttattg aaaatagtac
     3241 cgcccaattc cgccgccttc ggaccgtaaa atcctccttc tacacgaaga ttactggctt
     3301 tgaaacgttg aggattagtc gaaccatttc ccgaaagatt gatgccgctc tcccgagtgt
     3361 gtgctgtcgc gtagaaaacg ttgccctcaa tcttgccgtt ttcaatatgg aaagcaggtt
     3421 ctacaccgtt tttctccgtc agctttccgg aaatcgattt cctgccgaaa tcaacgtcaa
     3481 attctgcttt tgccgcttct ttatccgcct gattgtccca ttgaatgggt ttgctgatac
     3541 gcgcttccca agtgccggta taatgcgccg ttccggtttg cggaatgtcg gcttccgccg
     3601 tgcggatacc tttcaggaaa aggtcgatgt ccctgccttt agaggcttcc tgagtgggcg
     3661 ggatgctgcc taaaccgccg ttgccttctt ctgtcggcga ttcttcttcg gcttcttcgg
     3721 cttcttcggg ttcagcttct tcttcttcag cttcttcgat ttcttcttca tcttcttcgg
     3781 aaacttcgtc ttcatcgacg atttcgtctt cgccttcttc gccattatca atgtccgaat
     3841 cctcttcatc ctgcgccttc ggtttggcgg cgggacgttc ggtttgcatc cgtccgagtt
     3901 tcacataggt caaaaagtcg caacaagcac ggacggtcat tttcctgccg tcggcaagat
     3961 caatggtttg agtatctttg agcaaggaga tttcacgccc ttcgacaaga agtttgtcgg
     4021 gatgaccaaa atcgggcata gtggaaatgg caaacggtcg gggattttca ccacttgcct
     4081 catcaacgga aattttcaga gaatccaaga ttttggtgtg ttttccagac gtcagggcag
     4141 gttttgtatc tgctgcgttt gctgcctctg ttttttgttt gcctgcgaat acgccgaata
     4201 cgcttttgtc gttgctgata aaccgtccgg caagctcttc ccccttatcg ccgaaaaaac
     4261 cgccctcaag ccgctgatcg gcatcggcat ggaaaaacaa acgctcatta ccggcaaggt
     4321 ttttcgctaa atcaggattg accttggcac tgccggtaaa gcggttgccg tttaaatcgg
     4381 cagtgatgtt gtaaatggtc agcggttttt gcttctcact gggtttgaca tattggtttt
     4441 taatcagctc gcccgtcagg gttttgttac cgaaatctac cgtatattta gcaggatgtt
     4501 tttccctttc gtcgacatcc ttagccgcat aagaagttgc accaacatcg ttaccataaa
     4561 atgtgatata acccaaattg tcaatgccaa ccgccttacc tgctcgatga cgtttggcat
     4621 cggtcatata ttgccagtta ccagaatatt ccaccgttcc cgcactcggt aaagattggg
     4681 aaggacgttc tccggaataa tatacaaaac cgtcataacc taagcggtgg gtaacctgct
     4741 taccacctga gttctgttcg atttcatctg ttccaaatct agtataaaca tatccggccc
     4801 ggacatattc atactcagca tcttttgaat cggacgtgta gatttttaca ttaggatacc
     4861 gttcgttgat tttcttttta agtttgtcgg cctgttcttg cggcgtaccg tctaaaaaca
     4921 ggatatcctc ctctttgagc ggcagatact cctctgcctg cttactattg ggaatttcat
     4981 taccattttt atcaaaagct gcgatattcc gccttggcag ccgcattgcc gcaccgacgg
     5041 cgggccggtt gaccggcgtg gtttctaccg aagacccggc agggggcgaa gtgggaacgt
     5101 ccttagactt gaaagtgacg gggtacgcgg tcggcgttga ttcgacaaca ggctgcacgc
     5161 cgaaattgcc gccgatacaa gatgccaaaa gtaagggcaa caagacaatg ccgccataat
     5221 tcggtttaca catccctact tttcctccta tttgattaat aataattatc attatattaa
     5281 tatgtacaga taatatcaac ccgtttttat agtgaattaa caaaaatcag gacaaggcga
     5341 cgagccgcag acagtacaga tacatcccgt cattcccacg aacctacatc ccgtcattcc
     5401 cacgaacctg caccacgtca ttcccacgaa cctgcaccac gtcattccca cgaacctg
//
LOCUS       DAXIEB010000118         5373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000118 DAXIEB010000000
VERSION     DAXIEB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.118"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            74..547
                     /locus_tag="ACFOA3_001786"
     CDS             74..547
                     /locus_tag="ACFOA3_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6898745.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            673..1776
                     /gene="mnmA"
                     /locus_tag="ACFOA3_001787"
     CDS             673..1776
                     /gene="mnmA"
                     /locus_tag="ACFOA3_001787"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6898746.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            1847..3517
                     /locus_tag="ACFOA3_001788"
     CDS             1847..3517
                     /locus_tag="ACFOA3_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6898747.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3629..5263
                     /locus_tag="ACFOA3_001789"
     CDS             3629..5263
                     /locus_tag="ACFOA3_001789"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6898748.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1419 a         1480 c         1353 g         1121 t
ORIGIN      
        1 gtggtattta ttattcactt aatccactat aagattaagc acattccccg tctgatattc
       61 aaggagcaga aagatgaaaa aattattggc agccgtgatg atggcaggtt tggcaggcgc
      121 ggtttccgcg gccggagtcc acgtcgagga cggctgggcg cgcaccaccg tcgaaggtat
      181 gaaaataggc ggcgcgttca tgaaaatcca caacgacgaa gccaaacaag actttttgct
      241 cggcggaagc agccccgttg ccgaccgcgt cgaagtgcat acccacatca acgacaacgg
      301 cgtgatgcgg atgcgcgaag tcaaaggcgg cgtgcctttg gaggcgaaat ccgttaccga
      361 actcaagccc ggcagctatc acgtgatgtt tatgggtttg aaaaaacaat taaaagaggg
      421 cgacaagatt cccgttaccc tgaaatttaa aaacgccaaa gcgcaaaccg tccaactgga
      481 agtcaaaacc gcgccgatgc cggcaatgaa ccacggtcat caccacggcg aagcgcatca
      541 gcactaatct gctgaaaata tttgaaatgc cgtctgaaaa agcccgggct ttcagacggc
      601 atttttatga ccgcctttaa aatgtgttaa aatctgcctt taaaaaccgc cgtttccaac
      661 catattctac gcatgaatac gacagcaaac ccttccaata tcatcgtcgg gctctccggc
      721 ggtgtcgatt cttccgtaac cgccgccctg ctcaagcagc agggttatca agtgcgcggt
      781 gtgttcatgc agaactggga agacgacgac aacgacgaat attgcagcat caaacaggat
      841 tcgtttgatg ccatcgccgt tgccgatatt gtcggcatcg acatcgacat cgttaatttc
      901 gccgcgcaat ataaagacaa agtctttgct tattttcttc aggaatacag tgcggggcgc
      961 acgccgaatc ccgatgtgtt gtgcaacgcc gaaatcaaat tcaaatgctt tttggattac
     1021 gccgtagggc agggcgcgga taccatcgcc accggacact atgcgcgcaa agaagtccgc
     1081 aacggcgtgc attacctgct caaaggtttg gatcgaaaca aagaccaaag ctatttcctc
     1141 taccgcctca agcctttcca actcgagcgc gcgatttttc cgttgggcgg tttggaaaaa
     1201 cccgaagtgc gccgccttgc cgccgagttc aaactgccga ccgccgccaa aaaagacagc
     1261 acgggcatct gcttcatcgg tgaacgcccg tttcgcgagt ttctgcaaaa atacctgccg
     1321 accgacaacg gtaaaatggt tacgcccgaa gggaaaaccg tcggcgaaca cgtcgggctg
     1381 atgttctaca cgctcggaca gcgcaaaggt ttgggcatcg gcggcgcggg cgaaccgtgg
     1441 tttgttgcgg ctaaagattt gacgaaaaac gaactgattg tcgtgcaagg acacgaccat
     1501 ccgctgctct atacccgcag ccttgtgatg aacgatttga gtttcacgct tcccgaacgc
     1561 ccgaaggcag gacgctatac ctgcaaaacg cgttaccgta tggcggacgc gccttgtgag
     1621 ttgcgctatt tggatgatga aaccgccgag ctggtgtttg acgaaccgca atgggcggtt
     1681 acgccgggcc agtccgccgt gctgtacgac ggcgacatct gtttgggcgg cggcatcatc
     1741 caaacgaccg acaagcctgt catcatcaca cgataaaact tatgccgtct gaaacagttt
     1801 tcagacggca ttgttccgct caattccact ttaaagaccg atacctatga aaaaaatttg
     1861 gttagacagc tacgagaagg gcgtcagtgc cgaaatcgac atcacgcaat acaattccat
     1921 cagcgacgta ttccgccaaa gcgtggaaaa atttgcccgt ctgcccgctt ttcaaaatat
     1981 gggcaaaacg ctgacttatg ccgaaaccgg caaactggcg actgatttcg cctcttatct
     2041 gcaaaacgtc ctcaagctgc cgcgcggcga acgtgttgcc attatgatgc cgaacgtatt
     2101 gcaatacccg atagcccttt tcggcatttt gcaggcaggt ttggtggcgg tgaacaccaa
     2161 tccgctctat acgccgcgcg agctggagca tcagctgaaa gacagcggcg cgaccgccat
     2221 catcgttttg gaaaatttcg ccaacacgct ggagctggtg ctgccgcgca cgcagatcaa
     2281 acacgtcatc gtcgcctccg tcggcgaaat gttcgggctg cttaaaggtt cgctgatcaa
     2341 tttcatcatc cggaaaatca agaaaatggt gcccgaatac cgtattcggg aaaccgtttc
     2401 ctttcagacg gcattgaaag agggagcgaa gcacgttttc caacctgtcg cattaaaccg
     2461 cgaagatacc gcgctgctgc aatacacggg cggcacgaca ggtgttgcca aaggggcggt
     2521 gctgagtcac ggcaacatct gcgccaatat gcttcaggca aaagaatgga ttaaaaacca
     2581 gttgcgtgag ggaaaagaaa ccgttatcgc cgccttgccg ctgtaccata tttttgcctt
     2641 aaccgtgaat ctgatgattt ttgccaatgc cggctcgaaa atcatcctga ttaccaaccc
     2701 gcgcgatatg aaaggcttta tcggacaact gaaaaaacag cgggttaatg tatgtatcgg
     2761 cgtgaacaca ctgtttaacg caatggttaa ccgtcctgat tttgccgaag tcgatttttc
     2821 aggattgcgg ctgactttgg gcggcggtat ggcgacccaa aaagccgttg ccgaaaaatg
     2881 gaaaaaaatc accggcacgc ccatcgtcga agcctacggt ctgaccgaag ccagccccgg
     2941 cgtgtgctgc aaccccttaa acatcgaatc atacagcggc agcatcggtt tgcccgtctc
     3001 gtccaccgaa gtcgaactgc gcgacgcaaa cggcaaagaa gtccccgtcg ggcagccggg
     3061 cgaattgtgg gtgcgcggcc cgcaagtcat gaaaggctat tggaaccgtc ccgaagaaac
     3121 cgctaaagcc atagacgcgc gcggtttctt ggaaaccggc gatattgccg tcatggacga
     3181 aaaaggctgg ttgaagctgg ttgaccgtaa gaaagacctc attgttgttt ctggttttaa
     3241 tgtttatccg aacgaaatcg aggaagtcat cgcgcatcac ggcaaagtca tggaagtcgc
     3301 ctgtatcggc gttcccgacg aaaaaaccgg cgaggcactc aaagtattcg tcgtcaaaaa
     3361 agacccgtca ttgaccaaag aagaacttat cgccttttgc cgcaccgaat taaccgcata
     3421 taaagtaccg aaaaatatcg aattccgcga cgagttgccc aagtccaacg tcggcaaaat
     3481 cctgcgccgc gagttgcggc aaagtaccgg gaaataaaga aaagatgccg tctgaaaaca
     3541 gccgtccacc gttcagacgg catccgtccg tttgccagaa ccgcgcgctt cacgttaaaa
     3601 tcacgcattc caacatgggt attccatcat gaccaaattc attttcgtaa ccggcggcgt
     3661 tgtctcctca ctgggtaaag gtatcgccgc cgcttctatt gccgccatcc tcgaatcgcg
     3721 cggcttgaac gttaccatgc tcaagctcga tccttatatc aacgtcgatc ccggcacgat
     3781 gagcccgttt caacacggcg aagtgttcgt aaccgacgac ggcgcggaaa ccgacctcga
     3841 cttgggacac tacgaacgtt tcatcgattc cacgatgacc cgccgcaaca gcttcagcac
     3901 ggggcaggtg tacgaaaacg tcatcgccaa agaacgacgg ggcgactacc tcggcggtac
     3961 ggttcaagtc atcccgcaca ttaccgacga aatcaaacgc cgcatccacg aaggcgcggc
     4021 aggttacgat gtggcgattg tcgaaatcgg cggtacagtc ggcgatatcg aatctttgcc
     4081 attcttggaa gccatccgcc aaatgcgcag ccagctcggc cgcaacaata cattgtttgc
     4141 ccacctgagc tatgttcctt acatcgccgc tgcaggcgaa atcaaaacca agccgaccca
     4201 gcacaccgtt aaagaaatgt tgagcatcgg tttgcaaccc gacatcctga tttgccgtat
     4261 ggacaggaca atgcctgcgg acgaacgccg caaaatcgcc ttgttctgca acgtggaaga
     4321 acgcgcgatt gtcggcagct acgatgtgga cagcatctac gaatgccccg aaatgctgca
     4381 cgaccaaggc atcgataaca tcattaccga gcagttgcag cttaatgtgc agcaggcgga
     4441 tttgaccgcg tggaaaaaaa tcgtccacgc catccaaaac ccgaaacaca ccgtcaaaat
     4501 cgccatggtc ggcaaatacg tcgatttgac cgagtcctac aaatcgctga tcgaagcctt
     4561 gaaacacgca ggcattcaca ccgaaaccga cgtacaaatt acctttgttg acagcgaaag
     4621 catcgagaaa aacaacggcg acgtttctat gctcaaagat atggacgcca tcctcgttcc
     4681 cggcggtttc ggttcgcgcg gcgtggaagg caaaatcgcc gctgtgcgct atgcccgcga
     4741 aaacaacgtg ccatacttgg gcatctgcct cggtatgcag attgcgctga tcgaatacgc
     4801 ccgcgacgtg gcaggtttga aaggtgcgaa ttccaccgag ttcgacctca aatgcgccgc
     4861 ccccgtcgtt gccctgattg acgaatggca aactgccgac ggcagcgtcg aaacccgcga
     4921 cgaatccgcc gatttgggcg gcaccatgcg tttgggcgcg caagaagtcg aattgaaagc
     4981 aggcagcctc gccgccaaaa tctacggcag cgaacacatc cgcgaacgcc accgccaccg
     5041 ctacgaagtc aacaacaact acgttcctac gctggaacag gcaggtttgg tcatcggcgg
     5101 cgtatccgcc ggacgcgaac gcttggtcga aaccatcgaa ctgccgaacc atccttggtt
     5161 cttcgcctgc cagttccacc ccgagtttac ctccaatccg cgcaaagggc atcctttgtt
     5221 caccgcgttt gtcaaagctg cgttgaacaa taaaaaagcc tgataaagcg ttacttgatg
     5281 atcaaaatgc cgtctgaaag cctgaatgaa gactttcaga cggcattttt gcaaatcggg
     5341 gatcataaaa gccatcaatt taatttttat ggt
//
LOCUS       DAXIEB010000119         5165 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000119 DAXIEB010000000
VERSION     DAXIEB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.119"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..388)
                     /locus_tag="ACFOA3_001790"
     CDS             complement(<1..388)
                     /locus_tag="ACFOA3_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898749.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEI"
     gene            complement(404..3085)
                     /locus_tag="ACFOA3_001791"
     CDS             complement(404..3085)
                     /locus_tag="ACFOA3_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6898750.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTGAGAA
                     IIALAVTVVTAGAGAGAALGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKEL
                     GRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNG
                     GSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKIAHAVAGCAAAAANKGKCQD
                     GAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVNAAANAA
                     EVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTD
                     ISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNT
                     AAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISF
                     DTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEI
                     PTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTK
                     SISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
     gene            complement(3335..3718)
                     /locus_tag="ACFOA3_001792"
     CDS             complement(3335..3718)
                     /locus_tag="ACFOA3_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898751.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(3723..>5165)
                     /locus_tag="ACFOA3_001793"
     CDS             complement(3723..>5165)
                     /locus_tag="ACFOA3_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
                     /protein_id="HFC6898752.1"
                     /translation="INGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYITHKIA
                     HAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
BASE COUNT         1259 a         1104 c         1061 g         1741 t
ORIGIN      
        1 gtatttcttt acactttttt tgatattcag ggtgagctat taagaaatca gatattgcaa
       61 tattaaagta atgatatgct aaattaatag gtataattaa ttcttcagga ttgaaatcat
      121 ctccaactga aaaataaata gaaggagctt tatcaatata ctcaaatcca tttacctctt
      181 cataatctcc aatccatctg gtagcatcta ttttttcata ttgagcacct attactccaa
      241 aatgtccggt agttgtaata tttttaaaac actctaaaag cgtatcttta gtttcaaatt
      301 ggtaaattgt ttcaaaaaaa atcgaaataa caaaatggtt gttatttata gattttttat
      361 ttaagtttag aaaaatatta tttttcataa tttttccttt aaattattct gggtgaatgt
      421 ttgtaattct tcctgtattt acatcaaaat atgatctaaa tttgattccg tcaaaggttt
      481 ctgagaattg aatgacattt tttctttccg atattgattt agttctttca ttttgagcaa
      541 ttttagaggc ttttgaatat ccttgtgaag cagcattttg agccatttga agtattttat
      601 catcagaaaa ttttttagga ttataaacag tttttatact tgaaatttcc ttaaatccac
      661 catcaggttt acctgtcctg tctagtgtag gaatctcata tttaattcgg gtaatgcctt
      721 caatatcagt ttgggtttca gattttacgc gtcctcctcg tgaatttagt tctgccataa
      781 aattggtgcg gttatgggct cctttaatgc cctgtttttg actaaaacca tcagcatttg
      841 ccagatgtct ttttaggttt gtatcgaaac tgatgccttc cgggtatttg acattagtaa
      901 taggtgtatt ttgttttaca aaccctcttg gtattggaat gaatctagga ttaactcctg
      961 aaataagtgt ataaccttgg tcggacatcc attcggataa aggctttgta ttgcccgatt
     1021 gaagccaaga tttagcagca gtattcaaat gataagactg taaagccaaa tctgcttggg
     1081 tgtaagattt gctgaataat ttataccatt catcatcttt accaattaga cctgcttccc
     1141 aagatgaatg aaggcttcta ctatcgatca aatgttgttt tctgattgtt ctacattcag
     1201 tactacggga tatatccgta catattgcaa tcgaagcagc aagtctttta tcagcaacat
     1261 tttgatactt ttttacagta ttttttctgc acagttgagg attattctgt ttggcgcatg
     1321 cagtcatttc gttatcaaat tctctaccct ctttgtcgct aagctgatta tttttcaccg
     1381 ctacctcagc cgcattcgcc gccgcattta catcgccgcc gaccacaccg cttaccgtac
     1441 cggcaaccag tttgctgtat gccaaaatct gttcgcgttc tttagctgtc aaagtgtcag
     1501 gatttttgcc gtttgtcaaa gcctccccga ctatctcgcc cacagccgca cctatcgcac
     1561 catcctgaca cttgccctta ttcgccgccg ctgccgcaca gcccgctacg gcatgggcaa
     1621 tcttatgggc aatgtagtgc tgatccaatt gtttgatttt acttgctgcc tctccatgcg
     1681 cagtattcac caaagccgca aggatattcg cttccagatt gtctttcagg ctgccgccgt
     1741 tgacagcggt attaatcagt gcggcactgc ccgcattggc caggttgacg gtcaggttgt
     1801 tgatccactg cttatcgctg acattgttca gtgccgaagc accgattttg tcggctacgc
     1861 ctgcggtagc ggcggcaacc accagatttt tcaccgtgct gcttctgccc agctctttca
     1921 gggttttgcc gacatcgcct ttgttgttga tgaacgatac ggaagcctgg ctggccaaag
     1981 aggcaaatgc tgcatcggtt gcggcggcgg ccgcgccgtt taagcccagt gcggctccgg
     2041 ctcccgcgcc cgcagtaacc acggtaacag ccagcgcaat aatcgctgct ccggctccgg
     2101 ttaagccttc ctgtttatag tcccatttgt cgtacgccag ttgtacctgg ttccagttga
     2161 tgtttttcgc tacttgaagt tgtttcaaat aagcatactc gggctgcttg gcgagttttt
     2221 cgatttcggt tttcagattg cctttcggaa tgtcgacgat gtagccgccg ggagcagaga
     2281 gtacgggcgc aacggggccg gtgaaacttg gcagttgcag ggtttcgatg ttgctgccgc
     2341 gtcctgcctg tttttgccag agggcggatt tgctgctgct tacggtttct gtgtggatgc
     2401 tgcttttgat cccttcgagg ataatcttgg catcggcccg tgcctggtcg cctacgcctg
     2461 cacggatgac cgcgccgccc agcgtggttt caaactgcgt accttgtaac ttggtatccc
     2521 aacctgattg cagattagct gattcagcca ctacacggct tggtaaggca gtttgcatct
     2581 gttgggtaga ggtatcatgt accttatcat aagtaatgcc gagaaatttg cgtttcttat
     2641 gactgtctag tttgtcgtag ttgagctctt ctacggcata gagtgtcaat ttccgcccgg
     2701 cttcgatgtt aatgctgcct ttgggggcat cgaatacggt ggcgtaggcg tcgatgctgc
     2761 caccagattt gatgtcgata ccttgggatg cgctgaggtt tactgcgtcg ggcttggcgt
     2821 ttttgtgttc tttgacttcg gtaatgtgtt tgcggttgta ccatttgccg gttttgtagc
     2881 tgcggcgttc taaggtatag agttcgccct gtccggcgta ataggattgg tcgccgtagg
     2941 attgcagttt gattttgccg ttttctgcgg ctatatcggc ggcactcaaa atgatgcggc
     3001 tgttctcatt ggcatacggt gcgctaatgc tcacaccggt tttacccgaa agttctgcag
     3061 caagcatagg gggtgtcttc agcacgcacg catctgggca gtatataggg aatctgaata
     3121 tttacttgca taacaaatgc cgtctgaaaa attgtgagct tttcagacgg cattgagccg
     3181 taaatcatgg aacgcgtgcg tgctggagca cacaccttac gcatggattt taggtttcat
     3241 gcaggctaca gcttgctgcg gcggcggtat aaaagtattt ttagtgattt accgtttttg
     3301 gtaattttgc ccgacggggt taaaaataca gttgctactc ttgctgcatg atattggcaa
     3361 gatttaattt aaatgtttgt tgaatcttgt ttacatcctt agcaaaaata tgatagtcac
     3421 tcgaagaata gatagatttt attctatcca aattagtttg ttgataacat tttttcattt
     3481 ccgcgatgaa aaaattatct tttcctcttt cattgagaca gttattttct aacagataga
     3541 ttgcccatgc tgtaaaaata aatgtactat cttccggata ttcagataaa taaatttcga
     3601 attcaattat agaatattca gctgcttcaa ttaatgttat atctaaatct ctgacaaatt
     3661 tttccgttat atttttaaat aatttcttga attctatata attttttatt ttttccatga
     3721 ttctattttc ctgtaaaaat atttaattga atatttggat agcgatttct aaactctaaa
     3781 ataacattgc tgcaagattg acaggccttt aattctgtaa atagatcaat tctacctgat
     3841 acattacgat tatttccgag ttgctgtgct atagtttcaa gtaatttata ttctccatct
     3901 atatttctta atgttcctct aggaggagag gcaatattat gatatttatc aacagatata
     3961 ggtttaaaaa tttttgtttc aggtaacgaa ataaatccat gttccccttt ttggaaagaa
     4021 ctaaatgctt ccatcctttg cggtaatcct ttaacatcaa tttttgctat cgcaagattc
     4081 cctatttgtc gatactcttc cggcagtttc atgcggattc tctggctttc agcagttaaa
     4141 gcttgtctta aagcaggagc actgtcattg ctcagataac gggtaacagg tgcaggcttg
     4201 gccgatattg cagcctgtgc agatacagat cctgcaacca tcaatgtgcc attaacaacc
     4261 tgaactgtac catgcagata ttctccatta gagattgctt tcgctccctg tccaatgtta
     4321 tatcctcctg ctgccacagt agctgctttg acactgaagc caagcggtcc gtttaataag
     4381 taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa aagaatcata acgactcaat
     4441 gtttgatagg ctttcgataa ggtgttatta atatctttgg aaggaatagt gagattaccc
     4501 attgactggg gcgttttcag tatcgaagct gcccaagctg tgttacctcc agaggtttgg
     4561 ttaataaaat atgcaagaat atttttttct tgattattta aactacttgg atttttttga
     4621 tatcggtcaa gcaattgatt agaatatcga tctttctcaa tcaaatagat aaactgcctt
     4681 gcctcttgtt cggtcaggct gccccaatct aaagataata ggcctttccc tttggctttt
     4741 ttgatcaatt tttctctaag agcatactct tcttgagata ataggttatt ttcaatcgcg
     4801 actttagccg cattcgccgc tgtattcaca tcgccgccga ccacaccgct taccgtaccg
     4861 gcaaccagtt tgctgtatgc caaaatctgt tcgcgttctt tagctgtcaa agtggcagga
     4921 tttttgccgt ttgtcaaagc ctccccgact atctcgccca cagccgcacc gatcgcacca
     4981 tcctgacact tacccttatt cgccgccgct gccgcacagc ccgctatggc atgggcaatc
     5041 ttgtgggtaa tgtagtcttc gctgagatta aatttgattt tgctcgctac ttctccgtgt
     5101 acggtactga ctatcgcacc cagtgcggca tcgcccaagt tgtctttgag gctgccgccg
     5161 ttgat
//
LOCUS       DAXIEB010000120         5132 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000120 DAXIEB010000000
VERSION     DAXIEB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.120"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(99..1454)
                     /locus_tag="ACFOA3_001794"
     CDS             complement(99..1454)
                     /locus_tag="ACFOA3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6898753.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASGSVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1469..1741)
                     /locus_tag="ACFOA3_001795"
     CDS             complement(1469..1741)
                     /locus_tag="ACFOA3_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6898754.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1866..2603)
                     /locus_tag="ACFOA3_001796"
     CDS             complement(1866..2603)
                     /locus_tag="ACFOA3_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6898755.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2884..3783)
                     /gene="prmB"
                     /locus_tag="ACFOA3_001797"
     CDS             complement(2884..3783)
                     /gene="prmB"
                     /locus_tag="ACFOA3_001797"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6898756.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAHIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFDGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPKLALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3928..4521
                     /locus_tag="ACFOA3_001798"
     CDS             3928..4521
                     /locus_tag="ACFOA3_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898757.1"
                     /translation="MADGIGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDKKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4633..>5132
                     /locus_tag="ACFOA3_001799"
     CDS             4633..>5132
                     /locus_tag="ACFOA3_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6898758.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASIEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVSTCFKADKSAEAVNNDTEHNAT
                     YFSHFPS"
BASE COUNT         1408 a         1321 c         1135 g         1268 t
ORIGIN      
        1 cagccccata ttcttttcta aagacatttt acccgatgcc tataaacaaa aatgccgtct
       61 gaagcctttc agacggcatt tccccatcaa aaccgcaatc agtttttcat cgattgaacc
      121 ggagccggga ttctgccgcc tcggttaaca aacacttcgc acgaaccttc tttaaccggc
      181 atcacaggcg cgtagcccaa caggccgccg aactcgacgc tgtcgccgac ggtttttccg
      241 gttaccggaa taatgcgcac ggcagtggtt ttgctgttga tcatgccgat ggcggcttcg
      301 tcggcaatga tgccggaaat ggtgtgcgcg ggcgtgtcgc cgggaacggc aatcatatcc
      361 aagccgaccg aacaaacggc ggtcatcgct tcgagtttgt ccagcgtcag cacaccggct
      421 tcagcagccg caatcatgcc ttcgtcttca gaaacgggga taaacgcgcc gctcaaaccg
      481 ccgaccgagc cggaagccat cataccgcct tttttcacgg catcgttcag caatgccaaa
      541 gctgctgttg tgccgtgcgt accgcagacg ctcaagccca tttcttcaag aatgcgtgcc
      601 actgagtcgc cgacggcggg ggtcggcgcc agcgacaagt cgagaatacc aaacgggata
      661 ttcagcattt ttgaggcttc gcggccgatg agttcgccca cgcgggtaat tttgaaagcg
      721 gttttcttca ctacttccgc aacttcggtc aatgtcgttg catctgaatt ttccaacgcg
      781 gctttgacca cgcccggccc cgatacgccg acattaatca cagcatccgc ttcgcctgag
      841 ccgtgaaacg cgcccgccat aaacggattg tcttccactg cattgcaaaa cacgacgatt
      901 ttcgcgcaac cgaaaccttc gggcgtgatt tcagccgtgc gtttgatggt ttcgcctgcc
      961 agcttgaccg catccatatt gataccggca cgcgtgctgc cgatattgat ggagctgcac
     1021 acgatatcag tagttttcat cgcttcggga atggaacgga ttaacacctc atctgaaggc
     1081 gacatgcctt tttgcaccag cgcggaaaag ccgccgataa aggacacgcc gatggctttg
     1141 gctgccttgt ccaaagtctg cgccacgctg acgtaagaat cggctttgct cgccgccgcg
     1201 atttgggcaa tcggcgtaac ggaaatgcgc tgattcacaa tcggcacgcc gtatttggca
     1261 gacagatatt ttgccgtcgc caccaagtct ttgccgaccg tggtgatttt gttgtaaatg
     1321 ttttggttta acacgtcgat gtcggtgctg atgcagtcgt gcaaatcaat gccgatggta
     1381 atggtgcgca cgtcaaaatt ctggtcggcc accattttga cggtttctaa aatctcgccg
     1441 gattggatac tcatcacatt cctccaactc aaatgcggtg catcgcttgg aagatttctt
     1501 cgttttgcat acggatatca agcgcgagtt ttttgctctc ttccgcaaac aaatccaaaa
     1561 cctcttgacg cgatttgctg cattttgaag tgtccaccaa gataatcata gtaaaaaaat
     1621 cgtccatcag ctgttggctg atgttgagaa tattgatttg gttttccgcc aaaattttgg
     1681 aaacatcgta cacgatgccg acgcggtctt taccgatgac ggtgatgact gaattgttca
     1741 caggcttact ccttgcagat atccgttaaa gtccgaaatt ataccaccgt tggattttga
     1801 agaaatattg tcaacaatat atacatacaa aatgccggct gaaactattt cagacagcat
     1861 caaatttagg gttcgattaa ataaccatcc ttatcccact gggttttcct aaccaactta
     1921 tcatcctgat aaacagcctc gctcttttta gaaccatctt cataccactc caaaaccacc
     1981 ccgttgcgtt gatggtggcg gatagacagt tccgagagta atcggccgct ttcatcccaa
     2041 gtcagaattt tggcaggctc atcgttgacc ataaccattt ccgtcttgat gctgccgtcg
     2101 gcataccatt gcttccatac gccgtttgcc ttattttgct taaactggat ttcgctttcc
     2161 ttgccgccgt tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     2221 ataacggcag attttttacc gttcggatac cagttgaccc actccccgtc cggcttaccc
     2281 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     2341 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcagaa
     2401 tatttcttca tcgacggata ataaaaatcc tgcgcgtgcg caatacccgc caccacacta
     2461 tattgcctga tataagcggc agaagacatc gtcgccgtca gctttccgtt ctgattaaaa
     2521 taaacagaat aggtctgcgc cggcaaagcg gccgaaaaac ccaacaggac agttgaaaat
     2581 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     2641 atctgttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     2701 ctattctatt gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     2761 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     2821 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     2881 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     2941 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     3001 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     3061 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt tcggttcgtg
     3121 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     3181 aatcaaatca taagtgcctt ccaatccgtc aaacaaatcc gtatgaataa gccggatgcg
     3241 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     3301 atcaaccgca tcaatgtgcg catcgggata gtgatgcgcc atctgaatgg caaggcaacc
     3361 gcttccggtg caaagatcca aagcattatg caccagctca tcgtattcta tccaaggacg
     3421 aagtccgtta cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     3481 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     3541 gacagcacga cgctcaataa ccgccagcac ttcctctttt tcagcttcca agagttttgc
     3601 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     3661 cgcttcatca tgcgcattat ctgttccatg accaaaaaag agccctgcct cattaaaacg
     3721 gctgactgca aaacgtaaaa catcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     3781 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     3841 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     3901 aaaaccatct cccgaacgga aacggcgatg gcggacggta taggcaatct gattggctgg
     3961 gaaaaaacgg ggcttgttgt cggtaagcag tggataaccg caaaagacga caaggtgtcc
     4021 gatgtctgca atgccaacgg cgagatgggc gtaatcgggc tttacgagcc tttctcacac
     4081 ggcgcattga cgatacctgg tcatccgaac tgccgatgcg aggttgtttc cgtatcgggt
     4141 ggcgaattgg gggaatttgc cgaaaaaaag gagcttcgta aagcggctat gcagtatgcg
     4201 cgggataact ttatcggcaa aagctatgtc aatgaaaaca gcgggcatga actgaaggta
     4261 acttggcaag gtgtgaaaca cgctgcgtca aaggcaaatc aggcggaatt atccatcata
     4321 acaaaacttg atgacttatt gcgctacgca aaatatgagg gttcttattc ggataagaaa
     4381 ggtcatcctg atattattgc agcacataag tatcgtgccg ttgccaaggt tgggaatgag
     4441 tctttaaata tcggtgtgat tgtaagggaa tttccagacg gccataaaca ttacgaccat
     4501 ttcatcttga aggatgagta aagccctttt gcagtgtcgt tctggagcgg atagcgttaa
     4561 ggcaagtaca cttccagcct tacaaaaggg ctttaaattc agcatgccat ttatacaggc
     4621 aggagtaaac ccatgacaaa gttatacgca gaaatcgcca agagggaggc gcaggacgac
     4681 ggcacggtca aggttttggg ttatgcctca atcgaggcgg tcgattcgga cggtgaagtc
     4741 atcgcgacgg cagctatgaa ggcggcgatt cccgattata tgaagtttga cgcggggcgc
     4801 gagatgcacg gctcaaacgc tgcgggaacg gcaattgaaa tcaacgttga agacgacggc
     4861 ataacctttt tcggtgctca tatcatcgac cccgttgtcg tgtcgaaagt caaaacaggc
     4921 gtttacaaag gcttttccat cggcggcagc gttaccgccc gcgataattt gaacaagtcg
     4981 caaatcacgg gcttgaagct gacggaaatc agccttgtcg agcgccctgc caatcccgat
     5041 gcggtgtcta cctgctttaa ggcggacaaa agtgcggaag cggtaaacaa cgatacagaa
     5101 cataatgcta catattttag ccatttccct tc
//
LOCUS       DAXIEB010000121         4784 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000121 DAXIEB010000000
VERSION     DAXIEB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.121"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(6..1532)
                     /gene="ilvA"
                     /locus_tag="ACFOA3_001800"
     CDS             complement(6..1532)
                     /gene="ilvA"
                     /locus_tag="ACFOA3_001800"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6898759.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            1680..2849
                     /locus_tag="ACFOA3_001801"
     CDS             1680..2849
                     /locus_tag="ACFOA3_001801"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6898760.1"
                     /translation="MTAHKILPVLLSIILGISHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKTTEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(2972..3544)
                     /locus_tag="ACFOA3_001802"
     CDS             complement(2972..3544)
                     /locus_tag="ACFOA3_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6898761.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(3611..4594)
                     /locus_tag="ACFOA3_001803"
     CDS             complement(3611..4594)
                     /locus_tag="ACFOA3_001803"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6898762.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
BASE COUNT         1299 a         1299 c         1108 g         1078 t
ORIGIN      
        1 aagcgtcagg caagaaacag cttgtacgcg gcattttgcg tttcctcgtg atagctgtat
       61 cccagacttt ccaagaaacc gtcaaatgcg gcggcatcgt gcggcggcac atcgataccg
      121 accagaatcc gcccgtaatc cgcaccgtgg ttgcggtaat ggaagagcgt aatattccac
      181 cctccctgca tatggttcaa aaagcgcgcc aacgcccccg gacgctccgg aaactcaaaa
      241 ctgaccaaac gctcgttttc tactttgtcc gtccgccctc caaccatata acggatatgg
      301 attttggcaa tctcatcatc agtcaggtcg acattgggca atcccgcctc atccaaccgg
      361 ctaccgataa ccgccaaatc ctgcgggcct gccgcttgaa gtccgacaaa gatatgcgct
      421 ttttcatcgt ctccgtagcg gtagttgaac tcggtaatat tcctatttcc caatatattg
      481 acaaacttaa ggaagctgcc gcgttcttcg gggatggtaa cggcaaaaat accttcgttg
      541 ccctcgccca attcactacg ctccgacaca tggcgcaaac ggtgaaaatt catattcgca
      601 ccgctggtaa cggcaatcag ggtttggttt tccgcgcctt ctcgggcgat ataggctttc
      661 agacccgcca acgccaacgc gcccgccggc tcggtaatgc tgcgcgtgtc atcgaaaata
      721 tccttgaccg cgccgcaaac cgcatcggta tcgactgtaa tgatttcatc caaaagttct
      781 ttgcagagac ggaaggtttc gtttccgacg actttgaccg ccgtgccgtc tgaaaacagc
      841 ccgacatctt tcaaatggac gatttcgccc gcttcaaccg attgcttcat acagcaggaa
      901 tcgttggtct gaacgccgat aactttgatt tcggggcgga cctgcttgat aaatgccgcc
      961 acgcccgccg ccaaaccgcc gccgcctatc ggtacgaata cggcgcggat tggatcggga
     1021 tgctggctga caatttccat ccccaccgtc ccctgtcccg caatcacatc aggatcatca
     1081 aacggcgcga tataggttaa cccttctttt tccgccaact ccatcgcata atcgtaggca
     1141 tcgttgtatg aaacgccccg caaaaccacc tcgccgccac ggcttttgac cgcatccact
     1201 ttgattttcg gcgtagtctc cggcatgacg ataacggcac ggcaacccaa acgctgtgcg
     1261 gacaatgcca cgccttgtgc gtgattgccc gcgcttgccg caatcacgcc gcaagcgagc
     1321 gcatctttcg gcaacttgga cattttgttg tacgcgccgc gtattttgaa cgaaaaaacc
     1381 ggctgcaaat cttcgcgttt caaaaggatg ttgtttttca aacgtacaga aaggctgcgt
     1441 gccggttcca aaggcgtttc gaccgcgaca tcatagacag atgctgtcag gatgcggatg
     1501 aggtaattgg aataaggaag gggcgtgttc ataattcaat atgggataat cggtttatta
     1561 aaatcgcaaa acccaaaacc atacgcccaa gacggcgcga aagcaagaaa aatccgcccg
     1621 atcagacacc ctaagcgtat aatcggcagg ctgaaacact cacacaatta gaatatttca
     1681 tgacagcaca taaaatcctg cccgtcctgc tttccatcat cttaggcatt tctcacgcaa
     1741 cggccgcatc gcccgcgccc aacaaaccgg cggtacacgc cgcccccacg ttccaaacac
     1801 ccgaaaccct cacagcggca cacatcgtta tcgaccttca aagcaaacag attttatccg
     1861 ccaaaaacat caatacccct gtcgaaccgg cggcactaac ccaactgatg accgcatatc
     1921 tggttttcaa aaacatgaaa tcgggaaata tccgatctga agaaaactta aaaatacccg
     1981 aatccgcatg ggcttcagaa ggaagcagaa tgtttgtacg tcccggcgat acggtcagca
     2041 ccgacaaact cttaaaaggc atgattgcac tatccgcaaa cgatgccgcc ctaacccttg
     2101 ccggccgact gggcaacggc tcgattgaaa attttgtgca acaaatgaac aaagaagccc
     2161 gacgcttggg catgaagaac actgtattca aaaacccgac aggcttgagt agagaaggac
     2221 aggtttccac cgccaaagac cttgccctgc tgtctgaagc attgatgcgc gactttccgg
     2281 aatattaccc gctgttttcc atcaaatctt tcaaattcaa aaatatagaa caaaacaacc
     2341 gcaatatcct tttatatagg gacaacaatg taaacggtct gaaagccgga cacacagaaa
     2401 gcggcggcta caaccttgcc gtgtcatact ccggcaacgg caggcacatc cttgtcatca
     2461 cattgggttc ggaatcggcg gaaacacgcg catcagacaa cagcaagctg ctgaactggg
     2521 cattgcaagc cttcgatacg cccaaaatat atccgaaagg caaaaccgtt gcccaaatcc
     2581 aaatttccgg aggcagcaaa aaaaccgtcc gcgcaggctt cctcaaagaa gcctacatca
     2641 ctctgccaca taaggaagcg aaaacaaccg aacaaatttt ggaaaccata cagccgattc
     2701 ccgccccagt aaaaaaaggg caaattttag gaaaaatcaa aatcagacaa aacggataca
     2761 ccattgccga aaaagaaatc gtcgcactgg aaaatgtaaa aaaaagaagc cggtggcaaa
     2821 ggctttgggc gtgtctgaca gggcagtaat ctgccgtttc aaatatcccg tttttccaac
     2881 aaataaagaa atgccgtctg aaacacggtt cagacggcat aaaacaacag ggcggtacgc
     2941 atcatatacg taccgcctgc ttttcaatca attagcgttt tttaccggta acagtggcaa
     3001 cagccagatt ttcgttacgt ttcagggaaa cgctttctac accttcaggc agtttgatgt
     3061 ctgacaagtg cagaatgtcg ccggcaacca cttcggcaca atccaaatcc aagaaagcag
     3121 ggatgttggc aggcaaagca actacttcaa cagaagtgtt taacagagat acgcggccgc
     3181 cttgcagttt gaccgcttgg gaattttcag cgttaacgat gtgcagggga acacggatac
     3241 gtacaagttg atcggctttc acagcttgga agtcgatgtg ttggacttcg cggcggaacg
     3301 ggtgcatttg gaaatcgcgg acgataacgt ctttggtttc gccgttcaga gacaatttaa
     3361 tcaacgcagt atggaaagat tctttttcca atgcgtagaa tacagttttg tgatccacag
     3421 cgattgcaac gggctcttgg ccttcaccgt acaaaatgcc ggggatttgg ccttcgcgac
     3481 gcaggcggcg gctcgcacca gtgccttgtg cttcacgaac agaggcttga atttcataag
     3541 tcatgttaaa tactccaatt taaataaaac taccgtcatc ggccgcgacc agcttaagac
     3601 ggcttcgggc ttatggcagc aacatactgc ctgtcatcac ttcttcatta aagagatatg
     3661 aaacggattc ttcattgctg atgcggcgga cggtttcggc caacagaccg gcaatcgtta
     3721 cctgacggat acggtcgcag tttttagccg cttcagacaa aggaatggta tcggttacga
     3781 ccacctggtc gatttcggat gaggcgatac ggctgaccgc ctctccggag aatacggcgt
     3841 ggctggcata tgccaagaca cgttccgccc cccgctcttt cagagcgacg gcggctttgc
     3901 acagcgtatt tgcagtgtca atcatatcgt ccacaatcag acaggttctg ccttgaatat
     3961 cgccgatgat gttcatgact tccgccacat tggctttcgg gcggcgtttg tcgatgattg
     4021 ccaagtcggc attcagggat tttgccacgg cgcgggcgcg gacgacaccg ccgatgtccg
     4081 ggctgacgac ggtcagattt tcaatccgct gttgtttgat gtcgttcaac agaatcgggg
     4141 tggcataaat attgtccact ggaatatcga agaaaccttg aatctggtcg gcatgcaaat
     4201 cgacagtcaa aacacggtcg atccctgccg aatacagcat atttgccacc agtttggcag
     4261 aaatcggaac gcggacggaa cgcggacggc ggtcttggcg cgcatagccg aaatacggaa
     4321 tggctgtggt aatacgacct gccgaagcac gcttcagtgc atccgccatc gtcaggattt
     4381 ccatcaggtt gtcattggtc ggcgcacagg tcggctgaag gatgaaaaca tcgcgcccgc
     4441 gtacgttttc caacagttcg acggcaactt cgccgtctga aaacttggat acggaagcat
     4501 tgcccaaaga aatgtccaaa tgcctgacaa cacgttgtgc caattcggga ttggcattgc
     4561 ctgtaaatac catcaaactg tcgtacgcag ccatattctc acctgatttt atgtttaact
     4621 tccgctcaga aaacacaatg cttccctctg cttgtaccaa gcccgacatt atacctgcaa
     4681 catccgacac aaacaaaggc atttcaatat ttttatttct atgaaataaa agcgtgtaag
     4741 caggcttaca cgcttttatc tggctgggga ggaaggattc gaac
//
LOCUS       DAXIEB010000122         4782 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000122 DAXIEB010000000
VERSION     DAXIEB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.122"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(653..1699)
                     /gene="recA"
                     /locus_tag="ACFOA3_001804"
     CDS             complement(653..1699)
                     /gene="recA"
                     /locus_tag="ACFOA3_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6898763.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGVDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1953..2717
                     /gene="aroD"
                     /locus_tag="ACFOA3_001805"
     CDS             1953..2717
                     /gene="aroD"
                     /locus_tag="ACFOA3_001805"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6898764.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2737..4752
                     /gene="rep"
                     /locus_tag="ACFOA3_001806"
     CDS             2737..4752
                     /gene="rep"
                     /locus_tag="ACFOA3_001806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6898765.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRTKAETGEAGEFINSLLEEIDYENHLLASEEGKTGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         1175 a         1304 c         1281 g         1022 t
ORIGIN      
        1 cggcaagaat agcgggaatc ggcaggctga agcccaccct accattattt acacatccgt
       61 accgcttaaa tgccgtctga aacttcgtca ttcccgtgaa aacgggaatc caaccccgtg
      121 ggagcagaaa cttacacccc gtcattcccg cgaacgcggg aatccagtaa ccgaaaaacc
      181 acaggaatct atcggaaaaa cagaaaccct cgaccgtcat tcccgcgaac gcgggaatcc
      241 agtaaccgaa aaaccacggg aatctatcgg aaaaaacaga aaccctcgac cgtcattccc
      301 gcgaacgcgg gaatctagaa cgtagaatct gagaaaccgt tttactcgat aaatttccat
      361 gccaacaggt ctggattccc gccttcgcgg gaatgacggc atattttttg catttgatat
      421 aaagggtcgt ttgaattttg ttcagcaagt gcaaagtgtt gcacataaaa gggcgcaggc
      481 ttcatccgta aagtcggcgc aggatagagg caaagcaggc gtaggtcggg ctgtagcaac
      541 tgtatttttc accccgtcgg gcaaaaatac caaaactcaa atcaagccgt tcggaggcgg
      601 ctcaaaaaac ggtacttcgc agcagaagta ccgtttatcg ggatttcagg ttttattctt
      661 cggggcgttc gccgtcggtt tcgtcctgcg tcccttcggt gatgtgcatt tctacgccgt
      721 tgagggcgcg gatttttgcg tcgatttcat cagcaatttc gggattttcc ttcagccaga
      781 cgcggacgtt gtctttgccc tgaccgattt ttgcgccgtt gtagctgtac cacgcgccgg
      841 atttgttgat gatgtcgttt tttacgccga tgtcgatcaa ttcgccttcc caactgatgc
      901 cttctccgta gaggatgtca aactctgcct gacggaacgg gggggcgact ttgtttttga
      961 tgactttgac gcgggtttcg ttgcccaata cttcttcgcc ttttttgatg gatccggtgc
     1021 ggcggatgtc gaggcggacg gaagaataga atttcagcgc gttgccgccg gtggtggttt
     1081 cggggctgcc gaacattacg ccgatcttca tccggatttg gttgatgaac acaaccagcg
     1141 tgttggtttt tttgatgtgt ccggtcagtt tacgcaaagc ctggctcatc aggcgcgcct
     1201 gcagtccgac gtggctgtcc cccatatcgc cttcgatttc ggctttgggg acaagtgcgg
     1261 ctacggaatc gacgactacc atatctacgc cgccggagcg cacgagcgta tcgcagattt
     1321 ccaaagcctg ttcgccggta tcgggctggg agaggtaaag ctcttcgact tttacgccga
     1381 gtttgcgggc gtaaacggga tcgaaggcgt gttcggcatc gacaaaggcg cacacgccgc
     1441 cgtttttctg gcattgtgcg acggcttcca agcataatgt ggttttgccg gaagattcgg
     1501 gaccgaagat ttcgacgatg cgcccgcgcg gcagaccgcc gactccgagg gcaaggtcta
     1561 atccgagcga tccggtggaa atgacttcga ggttttcttc ctgctggctg ccgtccattt
     1621 tcatgatggc gcctttgccg aaactttttt cgatttgcgc cagtgcggcg gcaagggctt
     1681 tgcttttgtc gtctgacatt ggggttactc cggaacaaat gcggtatgtg ggatgcggcg
     1741 caacacgggc tgcggcgcgg gatttgtatc gttttcccga tgtgcgggct atcggtaatg
     1801 ctgcttcacg gggttgccat tatcgcatat ttccttgctt gccgatatgc ggcaggacgc
     1861 ggcggcttgt gccggaatgg aatctggatg ccgtctaaaa ggcggccggc tttgttataa
     1921 tggcggctgt tttttctgtg tgtgcctgtt ttatgtgttc ctgccttgtt gtcaaaaata
     1981 ccgttatcgg aagcggacgc accaaaatcg ccgtgccgct tgtcgcccgc gatgccgccg
     2041 tactttccgc cgtacttgac caaatcaaaa acctgccctt cgatatcgtc gagttccgcg
     2101 ccgacttttt ggaatgcgcg ggcagcatcg gcgaagtatt gcgccacacg cagaccgtcc
     2161 gcgacgcgct gcccgacaaa ccgctgctgt ttacgttcag acggcatggc gaaggcggct
     2221 cattcccgtg ttcggacgat tattattttg aactgctcga cgcgctgatc gaaagccgcc
     2281 tgcccgacat tatcgatatt gagctgtttt ccggcgaaac cgccgtccgg tgcgccgtgg
     2341 caaatgctca aaaaaacggc atcgccgccc tgctctgcaa tcatgagttt caccgcacgc
     2401 cgccgcaaga agaaatcgta tgccgtctga aacagatgga ggactgcggc gcggacatct
     2461 gcaaaattgc ggtgatgccg caaagcgcgg aagatgtgct gactttgctt tccgccacgc
     2521 tcaaagcgaa agagcttgcc gccaaaccga ttgttacgat gtcgatgggg cagacggggg
     2581 cggtcagccg gcttgccgga caggtgttcg gctcaagcat cacgttcggt tcgggaacgc
     2641 aaaactccgc gccggggcaa atcggcgtat ccgccctccg tgcgacactc gactgcctcg
     2701 aaaacggcgc agactgattt cagacagcat caaaacatga tgaaactcaa cccccaacag
     2761 ctcgaagccg tccgctacct cggcggccca ctgctcgtcc ttgccggtgc aggcagcggc
     2821 aaaaccggcg tgattactca aaaaattaag catttgattg tcaatgtcgg ctacctgccg
     2881 cataccgttg ccgcaattac ctttaccaac aaagccgccg cggaaatgca ggagcgcgtc
     2941 gccaaaatgc tgcccaagtc gcaaacgcgc gggctgacaa tttgcacgtt ccattctttg
     3001 ggcatgaaga ttttgcgcga agaggcgaac catatcggtt acaaaaaaaa cttctccatt
     3061 ctcgattcta ccgacagcgc gaaaatcatc ggcgaactct taggcggtac gggcaaagaa
     3121 gccgtattca aggcgcagca ccagatttcc ttgtggaaaa acgatttaaa aacgcctgaa
     3181 gatgtcgttc agacggcatc gaacatttgg gaacaacaaa ccgcacgcgt gtatgcgagc
     3241 tatcaggaaa ccttacaaag ctatcaggca gtggacttcg acgacttgat ccgcctgcct
     3301 gccgtgctgt tgcagcaaaa cagcgaagtg cgcaacaaat ggcagcggcg gctgcgttat
     3361 ctgctggttg acgaatgcca agatacgaat acctgccaat ttacgttgat gaaacttctg
     3421 accggcgcgg aaggtatgtt taccgccgtc ggcgacgacg accagtccat ctacgcatgg
     3481 cgcggcgcga acatggaaaa cctgcgcaaa atgcaggaaa actatccgca gatgaaggtc
     3541 atcaaactgg agcaaaacta ccgctccacc gcgcggattc tcaaaatcgc caacaaagtc
     3601 atcgagaaca accccaagct gttcaccaaa aaactttggt cgaaattggg cgaaggcgag
     3661 cccgtcaaag tcgttgcctg ccaaaacgag cagcacgaag ccgactgggt cgtcagccaa
     3721 atcgtcaaac aaaaactcat cggcggcgac aaaacccaat atgccgattt cgccgtgttg
     3781 taccggggaa agcatcaggc gaggattttc gaggaagcat tacgcggcgc gcgcatcccc
     3841 taccgtctct ccggcggaca aagctttttc gacaaagccg aaatcaaaga cgtgttgtct
     3901 tatgtgcggc tgcttgccaa ccccaacgac gatcccgcct ttctgcgtgc cgttaccacg
     3961 cccaaacgcg gcatcggcga tgtaacgctg ggcaagctca acacttacgc gcacgaacac
     4021 gaatgcagcc tgtatgaagc cgcgcaaaac gaagaagccc tcgccacgct gaacaatacc
     4081 aaccgccaac acctgcaagc ctttatggat atgttcggca gctaccgcac caaagccgaa
     4141 accggcgaag cgggcgagtt catcaacagc ctgctcgaag aaatcgacta tgaaaaccac
     4201 ctgcttgcca gtgaagaagg caaaactggc gaaatcaaat ggcgcaacgt cggcgatttg
     4261 gtatcatggt ttgcgcgaaa aggcggggaa gacggcaaaa acatcatcga actcgcccaa
     4321 accgtcgcct tgatgacgct tttggaagga aaagacgaag aagaaaccga tgccgtctcg
     4381 ctatccacgc tacacgccgc caaaggtttg gagtatccct atgttttcct tgtcggttgc
     4441 gaagaaggtg ttttgccgca caacgacagc atcgaagagg gcaacgtcga agaagaacgc
     4501 cgcctgatgt acgtcggcat cacccgcgcc aaacgccagc ttacgctgac ccactgcgtc
     4561 aaacgcaaaa aacaaggcat atggcagttc cccgaaccca gccgattcat agacgaaatg
     4621 ccgcaggaag atttgaaaat cctggggcgc aaaggcggcg aaccgattgt cagcaaagaa
     4681 gaaggcagac gcaaccttgc cgatataatc ggaaggctcg acagcatgaa aaaaggcggt
     4741 ccggaggatt aaaccaagat agggaaacga agccggagac aa
//
LOCUS       DAXIEB010000123         4776 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000123 DAXIEB010000000
VERSION     DAXIEB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.123"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            336..1256
                     /gene="ribF"
                     /locus_tag="ACFOA3_001807"
     CDS             336..1256
                     /gene="ribF"
                     /locus_tag="ACFOA3_001807"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6898766.1"
                     /translation="MKIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLGFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPDHR
                     YALGGVFVVDADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            1398..4187
                     /gene="ileS"
                     /locus_tag="ACFOA3_001808"
     CDS             1398..4187
                     /gene="ileS"
                     /locus_tag="ACFOA3_001808"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6898767.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYSVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1150 a         1310 c         1276 g         1040 t
ORIGIN      
        1 tagaatctcg gggtttgagc aactgttttt atccgataag tttctgtgcg gacaggtccg
       61 gattcccgcc tgcgcgggaa tgacggcgga gggttgcttg tctcggttta cctggttaaa
      121 aaagaacgat tttcactgat gttgcatcag gtttggggcg atgtttcaac acataggatc
      181 gcgcctgatg cacgctttat gcgtttggcg cgttcggcgg cgggaaattt gcctgctttt
      241 ccagcgtcgg gcgggcgtaa cgggcggcac actgtctata aaccgcaata ccgtttacaa
      301 tgaccgcctg tttcaccaca tacccgaacg caacaatgaa aatcaggctg gggcggcaca
      361 acgcgcccga ctttccacag ggtgccgccg taaccatagg caatttcgac ggcgtacacc
      421 tcggacacaa acacatcctc caaaaactcc gcctcgaagc cgacgcgcgc ggactgcccg
      481 tcgttgccgt catcttcgaa cctcaaccca aagaattttt tgccgccctc gcaggcaaaa
      541 ccccaccctg ccgcatcagc cccctgcgca ccaagctcga attattggaa ggcacaggct
      601 gtgtcgatgc cgtctgggtt ttgggtttcg atcaaaattt ttccgaaata tccgcgcaag
      661 ggtttatcga ccgcctgctg cgtcaaacct tgaatacgcg ttatttgctc gtcggcgacg
      721 atttccgttt cggtgcgggg cgggaaggct gttttgaact tttggcacaa cagcccgata
      781 tgcagaccga acgcacgcct tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg
      841 tccgccaagc cctttcagac ggcaacctcg cttatgcgaa aaaacttttg ggacacgact
      901 acgttttggg gggcagggtg gtgcacggca gaaaactcgg acgcacccta aacgccccga
      961 ctgccaacat ccgcctgccc gaccaccgtt atgccctcgg cggcgtgttc gtcgtcgatg
     1021 cggacggcgc attcggcacg cggcgcggcg tggcgagctt cggcttcaat cccaccgttg
     1081 atagcggctg ttctcaaaag cttgaagtcc acctgttcga ctttcaaggc gacctgtacg
     1141 gacaacggct gaacgtccgc ttcctgcaca aactgcgcga tgaggaaaag tttgacggta
     1201 tggaagaact gaaaaggcag atagcagccg atatggaagc cgcaaagtgt tggtagaaaa
     1261 accttataca aaccatccga ttgggctaca attaatcttt taacttttca gacggcatag
     1321 gggtttcccg ttgtgaaata ctgtttgagg cgcaatgccg tctgaaaccg aaatattgta
     1381 acaatagaga ttaaaaaatg accgattaca gtaaaaccgt aaacctgctc gagagcccgt
     1441 ttccgatgcg cggcaacctt gccaagcgcg agcctgcatg gctgaaaagc tggtacgagc
     1501 aaaaacgcta ccaaaaactg cgcgaaatcg ccaaaggtcg tccgaagttc atcctgcacg
     1561 acggcccgcc gtatgccaac ggcgacatcc acatcggtca tgccgtcaat aaaattctca
     1621 aagacatcat tatccgcagc aaaacccaag ccggttttga cgcgccttat gtaccgggct
     1681 gggactgcca cggcctgcct atcgaagtga tggtggaaaa actgcacggc aaagacatgc
     1741 ctaaagcccg tttccgcgaa ttgtgccgcg aatacgccgc cgaacagatt gcccgtcaga
     1801 aaaaagactt tatccgcttg ggcgtgttgg gtgactggga ccatccttac ctgaccatgg
     1861 atttcaaaac cgaagccgat accgtgcgta tgctcggcga aatctacaaa tccggctatc
     1921 tctaccgggg tgcgaaaccg gttcaattct gcttggattg cggctcttca ttggccgaag
     1981 cggaagtgga atataaagac aaaatctcgc ccgcgattga cgttgcctat ccgtttaaag
     2041 acaccgccgc gcttgccgcc gcattcggtt tggcaggtat cgaaggcaaa gcgtttgccg
     2101 ttatttggac aaccacgcct tggactttgc ctgcgagcca agctgtttca gcgggtgcag
     2161 acgtggtgta tcaattaatc gatacgccca aaggcaaatt ggtattggcc aaagatttgg
     2221 cggaagacgc tttgaaacgc tacggctttt cagacggcat ttctattctt gccgaaacca
     2281 ccggcgacaa gctggaaaac ctgcacatga accatccgtt cctcgaacgc gatattccca
     2341 tgcttaacgg cgaacacgtt accaccgatg ccggtaccgg cttggtgcac accgcccccg
     2401 cgcacggttt ggaagactac gccgtctgca acaaatacgg catcgagctt tacaatcctg
     2461 tcaacgccga aggcaaatac atcggcgaaa cgccgcgcgt tgccggtatg cgcgtttggg
     2521 aggcgaaccc cgtcatcctg caatggttgg aagaaaccgg caaccttttg gcaagcagta
     2581 aaatcgaaca cagctacgcc cactgctggc ggcacaaaac gccgctgatt taccgcgcga
     2641 ccggtcaatg gtttgtcggt atggacaaag ccggtgccga cggcaaaacc ctgcgcgaca
     2701 aagccatcaa ggccgtggac gacaccgaat tcttcccgtc ttggggtcgc gcgcgtttgg
     2761 aagccatgat tgaaggtcgt cctgactggg tggtttcacg ccaacgctat tggggcacgc
     2821 cgatgacttt ctttgttcac aaagaaacgg gcgagctgca tccgaactct gccgaacttt
     2881 tggaaaaagt tgccctgaaa atcgaagaaa aaggcatcga ggcttggttc tccctcgata
     2941 aaagcgaatt attaagcgcg gaagattgcg aacattacga caaactcccc gacacgatgg
     3001 acgtatggtt cgactccggt tcgacccatt attccgttgt gaaacagcgc gaagaattgg
     3061 actggcctgc tgacttgtac ctcgaaggca gcgatcaaca ccggggctgg tttcagtcgt
     3121 ccatgctgac cggctgcgcc tcatcaatgg gtcgcgcgcc gtacaaacag ctgctgaccc
     3181 atggtttcgt ggtggatcaa aacgggcgca aaatgtcgaa atccatcggc aacgtcgtcg
     3241 cgccgcaaga agtgtataac gagttcggcg cggacatcct gcgcctgtgg gcggcatcca
     3301 ccgattacag cggcgaattg gcgatttcca aagaaatcct caagcgcgta accgaaagct
     3361 accgccgtat ccgcaatacc ttgagctttt tgtttgccaa cttgagcgac ttcaatccta
     3421 ttgaagatgc cgtgcaacag gcggatatgg tggaaatcga ccgctactcc gtggtattgg
     3481 cacgtcagct gcaagagcgt ctggcaggcg attactatcc gcgttatgcc ttccactttg
     3541 ccgtaaaaga cattgtttct ttctgctcgg aagacttggg tgcgttctac ctcgacatcc
     3601 tgaaagaccg cctctacacc accaaagccg acagccatgc acgccgcagc gcacaaactg
     3661 ccctgtatca catcacacgc agtttggttc tcttgattgc accgattttg tgcttcaccg
     3721 gcgaagaagc gtgggacatc atcggcggcg gcgaagaaga cagcgtcctc ttccatactt
     3781 ggcacgagtt cccgaccatc aacgaaaaaa ccgaagccga actggtgaaa aaatggacgg
     3841 caatccgcga agcccgcgaa gcggtaaccg ccgccatcga gcctttgcgc gccgacaaaa
     3901 ccgtcggttc gtccttgcaa gccgaagccg aaatcaccgc gccggaagaa atggccggct
     3961 acctgaacgc cttgggcgaa gaattgcgct ttgccatgct ggtgtctaaa gcagaagtga
     4021 aagtaggcag cgaacttgcc gttgccgcta aagccagtga tggtgaaaaa tgcgaacgct
     4081 gctggcacta cacccgcgat gtgggcgcgg ttgcaggcta tgaaaccgtc tgcaaacgct
     4141 gtgcagagaa tgtcggcgga gaaggcgaaa cgcgccatta cgcctgataa agtttgagca
     4201 aatgccgtct gaaaccgccg aacagcattt cagacggcat tttttgtgcc gcgatttgtc
     4261 tttataacgg cggaggggtt tcaagattgc ggtgttgtcg gaatgcaact gaaccgtcat
     4321 tcccacgaaa gtgggaatct agaatctcga agtttcagtc atttccgata gattcccgcc
     4381 gcgtcgtggg tctggattcc cgcctgtgcg ggaatgacgg atttcgagat tgcggtgttg
     4441 tcggaacgca actgaaccgt cattcccacg aaagtgggaa tctagaatct cggggtttca
     4501 gtcatttccg atagattccc gccgcgtcag gggtctggat tcccgcctgt gtgggaatga
     4561 cggatttcga gattgcggtg ttgttggaac gcaactgaac cgtcattccc acgaaagtgg
     4621 gaatctagaa tctcggggtt tcagtcattt ccgatagatt cccgccgcgt caggggtctg
     4681 gattcccgcc tgcgcgggaa tgacggattt caagattacg gtgttgtcgg aatgtaactg
     4741 aaccgtcatt cccacggaag tgggaatcta tagtgg
//
LOCUS       DAXIEB010000124         4750 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000124 DAXIEB010000000
VERSION     DAXIEB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.124"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            133..528
                     /locus_tag="ACFOA3_001809"
                     /pseudo
     CDS             133..528
                     /locus_tag="ACFOA3_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            734..967
                     /locus_tag="ACFOA3_001810"
                     /pseudo
     CDS             734..967
                     /locus_tag="ACFOA3_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(895..1548)
                     /locus_tag="ACFOA3_001811"
     CDS             complement(895..1548)
                     /locus_tag="ACFOA3_001811"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC6898768.1"
                     /translation="MASIILNYLEKDGRPVIHGMGYISEWKINNGKVFYNISDTILNE
                     LLLKSGRSMFLLMDWSVFNSLPGKYCGILYKLCKDYAGIGKTPYFTVQEFRHYLGLND
                     KEYEEFRALKAKIIKPAIEHINNSENVDIKIIPQLKRENRRVVGLHFDIEYKTNIEKN
                     NQNMADEDIETAFSESKIEPLATLIKFLETKTPRSSSGLERLCHCPSRTALNMDNSG"
     gene            complement(1983..2705)
                     /locus_tag="ACFOA3_001812"
     CDS             complement(1983..2705)
                     /locus_tag="ACFOA3_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6898769.1"
                     /translation="MVTDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASVSLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNLVKEIEVARVLQNIGVQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGADEDIDFPDSPNIG"
     gene            complement(2779..3363)
                     /locus_tag="ACFOA3_001813"
     CDS             complement(2779..3363)
                     /locus_tag="ACFOA3_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC6898770.1"
                     /translation="MSPTHEISNVERLIDEINRIHAGYHERVSKLYGGNLPNLSKTLR
                     HLDIDFVLQYRLNLHESINDYLIRSDIGDLPLAYYYRVKTTESVFDKIRWFSERESGY
                     PANKVLNDIFGVRIIADSALMEEIHAALDEWVSTKGLKNWNVQEKNGYIGTHVYFNQK
                     NNLFFPWELQIWDKADALKILRAMNGTSDFLFNL"
     gene            complement(3676..3948)
                     /locus_tag="ACFOA3_001814"
     CDS             complement(3676..3948)
                     /locus_tag="ACFOA3_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898771.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(4136..4624)
                     /locus_tag="ACFOA3_001815"
     CDS             complement(4136..4624)
                     /locus_tag="ACFOA3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898772.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVDFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYPQKDKVILFPKFGEYDLVLNPD
                     II"
BASE COUNT         1382 a         1014 c          831 g         1523 t
ORIGIN      
        1 agagttatta gaaaaaattg gatagtctga ccattctaga tcaaggattt tggcgagtca
       61 attaccgcca ttttactgcc atttgtttat taaattaggg actttactag ataacggtta
      121 aaaatcccat tcgaacgaaa tggcaaggtt tataccgtcg ttgtccctaa tgcgcaatca
      181 gcaacattat tgccgattat ccgagagaaa gttaagtctg atggcattgt gtatacggat
      241 acctttcgta gttatgatgt acttgatgtc agtgaattta gccatttacg caagttttcc
      301 agtatttgac tggcaattta aaacagtcgg attttgtccc atttgttggc caagtcttta
      361 cttgcttggc cgtttgaatt taaaaagcag tctttctact ttccgacctt ttttctgttg
      421 taaggtctat gatccaatag cattcccaaa gagcattttg gacggtattt aaaggaatgt
      481 gaatagcgtt ttaacaacag tgaaatagaa tctaaaattc agatttgaag acaattagtt
      541 aatgggaatt tggtctggtt atctagtaca gcctctaaaa ttttagtgac cgaacaaact
      601 cgaaaaatcc acatcgccga tgcgttccag ccggtcggca aagctgtcaa gattcacaga
      661 ttcagcgttt gagacctttg caaaattccc caaaatcccc taaattccca ccaagacatt
      721 taggagattt ctcatgagca ccttcttcca gcaaaccgca caagccatga ttgccaaaca
      781 catcgaccgt ttcccgctat tgaagttgga ccaggtgatt gattggcagc cgatcgaaca
      841 atacctgaat cgtcaaagaa cccgttacct tagagaccac cgcggccgtc ccgcctaccc
      901 gctgttgtcc atgttcaaag ccgttctgct cggacaatgg cacagcctct ccaatcccga
      961 actcgaacgt ggtgttttgg tttccagaaa tttaatcagt gtggctagcg gctctatttt
     1021 cgattcagaa aaggctgttt ctatatcctc gtctgccatg ttctggttgt ttttttctat
     1081 atttgttttg tattcaatat caaaatggag tcccacaacc cgcctatttt cccgttttaa
     1141 ttgcggtata attttgatgt ccacattttc actgttgttg atatgctcta ttgccggttt
     1201 aattattttg gccttcaacg ctctaaattc ttcgtattct ttgtcattca atcccaaata
     1261 gtgtctaaat tcttgaacgg taaaataggg ggttttgccg atgcctgcgt aatccttgca
     1321 caatttgtat aggattccgc aatattttcc cggtaagcta ttgaacacac tccaatccat
     1381 cagcaaaaac atgcttcttc cgcttttcaa tagtaactca ttaagaatgg tgtctgatat
     1441 attgtagaaa actttgccat tgttgatctt ccactctgaa atatagccca taccgtgtat
     1501 gacgggacgg ccgtctttct ccaaataatt caaaatgatt gatgccattc gtagagcatc
     1561 caagtctttt ttagttgttt tctttgaagt tgatatccaa tacaacgttc cagatctaag
     1621 atatctatcg caacaaaacc gttgtcatca taagtaccac cttattcaaa atgtgtttta
     1681 atcttctgca aaaaaagata ccaatacttg tattgcattt cagtcaagga atggcccatg
     1741 tggattaagg attgcggtgt tttttgttta tcaatatggt ttgatgtcat tttgatttcc
     1801 atttcatgtg gctactatat ctctctatta tgcactaata cacagagttt cttgatgtta
     1861 gaacgaatac ggcgtccgtg tcggcgtgaa attctgattt tccttatacg aaaccgcaaa
     1921 cctttattac atctcctgaa taatttagga tactttttag gtattgaaaa acacctgggt
     1981 ttttagccta tgttcggtga gtcggggaaa tctatatctt cgtccgcgcc gaaatagtct
     2041 atgcccgata tacaattttg atacacaaac ttggaaatat cggtatcgtc gccagagcga
     2101 tagaatgcgg acagtttttc attgaattgc ggcatctgca tttcggagat ttccaagatg
     2161 ccacggcccc tctccatcat cagaccattg gcaaataagg ttgccgttcg tttgttgccg
     2221 tcccaaaaaa cctgctgccg catacaataa agcataaaac ggacggctgc ctccgtcgtc
     2281 gaaccgcttt gcactccgat attttgtaac acgcgggcca cttcaatttc cttcaccaga
     2341 tttggggcat gacgggaacc gtccaatagc gttacgccga ccgaaccggt acggaaatca
     2401 ccgggtgcca aagaatcgtc cttggcaaca atgttgttga tttttttaag gagtgaaata
     2461 ttgaccggtt cgccatttga aatatgcgaa atcacatatt gataggcacg tttcaggttc
     2521 aagatggttt ggatgtcttc aagtgacacg gaggctacat ttttgccatg gataatttgc
     2581 tcagtctgca ataaggtcgt ctgacaattc tcaaaacggc tgaggttgtg aatttgggca
     2641 accaatactt tctttgccag aaaaatattt tcttccaaac tcaacttgta tttgtccgta
     2701 accacgagag ttcctttaat aattaaatct gtacacccaa ggtatgcacg gtaaacggtg
     2761 ctatattaaa aagacccatt aaaggttaaa caaaaagtcg cttgtcccgt tcatggctct
     2821 caatattttc aaggcatccg ccttgtccca tatctgaagt tcccacggaa aaaacagatt
     2881 atttttttga ttaaaataca catgcgttcc gatatagccg tttttttcct gaacgttcca
     2941 gttttttaag cccttcgtcg aaacccattc atctaaagca gcatgtattt cttccattag
     3001 tgcggaatct gcgatgattc tcaccccaaa aatatcattt aacaccttat tcgctggata
     3061 cccggactcg cgttccgaaa accagcggat tttgtcgaaa acggactccg tggttttaac
     3121 gcggtagtag tatgccaaag gcaagtcccc gatgtcagat cgaatcagat agtcgttgat
     3181 tgactcatgc aaattaagtc ggtattgcaa cacaaaatcg atgtccagat gccggagcgt
     3241 ctttgacaaa ttgggaaggt ttccgccata cagtttggaa accctttcgt gatatccggc
     3301 gtgtatgcgg ttaatttcgt caatcagccg ttctacatta ctgatttcat gagtggggga
     3361 cattccttgt tactctttca ttccaattat atgaaaataa catactaatt gctttcaact
     3421 atatattata caaaacaaat ttcaaaactt ccttttaatt tttcattacc gttaatacgc
     3481 taaaatagga ggaattgcaa atccaaaaaa catacacggt aacagattac aggtcatcaa
     3541 tgaatccaat ccatcccatg aagacaaagg agcgactact cggcctccaa gcagcctccc
     3601 gtactgaaaa cgacattgca ttaaagacag cgccgtttga tggctaatcg tacctacgca
     3661 ggcttaaaat ttccatcaat ccatatcatt tgagttttca tcttcataat atggatgatc
     3721 aaacaaatct ccttcccaaa tacattcact cgttatacct tgggattcca tgaaaggaac
     3781 ataagaataa aaatcttttt catattctcc ataaaatata ttcatacctt tgagccatac
     3841 tgcatcgcat tcatcacaca gaataatttc atcatgaaga tatttaggtt tcgaatggta
     3901 gagatagcct tgttgacaag caggacacat tttcattctc ttattcatga ttactccata
     3961 taatcgattt atttttgtgg gtaagcagta gtgatttgat tgctgccttt agtctgtaca
     4021 acaatacgta tatagcaacc tgataatttc ctggttttta tcagctttat cctcagattc
     4081 caaacccaat aataaaagcc gtctgaaatt tttttcagac ggccttaaaa cttaattaaa
     4141 taatgtccgg atttaacacc aaatcatatt caccaaactt tggaaataga attaccttat
     4201 ctttttgggg atataattct gttttttcat atcgctcatt attaataaaa tcctctttca
     4261 aaacatagca atcatccgta ttttcattga agaaataagt atatccttct gccaaatcat
     4321 gagattccaa agttatataa tctctccata cagccataga aagaaaataa taattcgaat
     4381 tggaatattt ttgaaattga tgggttaaat aatcccttcc aaatttatcg aaaaaatcta
     4441 ccgaaaaaaa acctacttcc tctcgcaagg taattacata ttcaggttgt gtcgccgaac
     4501 ctattacggc agtatataac tcccctttaa gatgtttttc ctcagcttgt ttctcagaaa
     4561 aaggcttatg aagtctctta taaccataag accactgctc acaataaaaa atatctttct
     4621 tcatttgtaa atccttcctg ttgtatctct aatgataaca ttttcttctt gtgccgccct
     4681 taatacagat tgtggaattg gattgacctg agcaggaact tctaaaataa gttttccttc
     4741 aagctctctt
//
LOCUS       DAXIEB010000125         4716 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000125 DAXIEB010000000
VERSION     DAXIEB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.125"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..263)
                     /locus_tag="ACFOA3_001816"
     CDS             complement(<1..263)
                     /locus_tag="ACFOA3_001816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898773.1"
                     /translation="MQFGLRQGSGCLIPSVTSGIAEFVGNGRCGLGGVLPNACVTSLC
                     RSISLATASLSKLFFRRPFIKYYRQRLNANFKPAPISSGLLSN"
     gene            complement(348..728)
                     /locus_tag="ACFOA3_001817"
     CDS             complement(348..728)
                     /locus_tag="ACFOA3_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6898774.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1205..3286
                     /locus_tag="ACFOA3_001818"
     CDS             1205..3286
                     /locus_tag="ACFOA3_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6898775.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIAKYVMQLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTLLFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3276..3470
                     /locus_tag="ACFOA3_001819"
     CDS             3276..3470
                     /locus_tag="ACFOA3_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6898776.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(3666..3857)
                     /locus_tag="ACFOA3_001820"
     CDS             complement(3666..3857)
                     /locus_tag="ACFOA3_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898777.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            3986..4546
                     /locus_tag="ACFOA3_001821"
     CDS             3986..4546
                     /locus_tag="ACFOA3_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC6898778.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1130 a         1354 c         1149 g         1083 t
ORIGIN      
        1 tcattggata acagccctga agaaatcgga gcaggtttaa agttggcatt gagccgctgc
       61 cgataatatt tgataaaagg tcgtctgaaa aacagctttg acaaagacgc ggttgccaaa
      121 gagatcgacc tacaaaggga agtaacgcag gcgttcggca aaacgccgcc caagccgcag
      181 cggccgttgc cgacgaactc ggcaataccc gaagttacgg acggtatcag gcagcccgaa
      241 ccctgccgga ggccgaactg caaaacaaat ccgacacaaa aaaccgtccg tccgacacaa
      301 aaaaccgccc gaaaaatccg gacggcggtt caaacaggct gccccgttta acgggcgcgg
      361 caggaagttt cgaccgaatt gccgtaggca tcggtaaagc cgaaaaaggc ttcgccgcct
      421 ttctggtgcc actcggttgc gtttccgaac aaaccgtgtt cggcggtata gcgttcgccg
      481 gatgcggcaa cgtcggaaga gaggacggca cgcctgccgt ccagccgcaa cgcgactttg
      541 ccgctgtcca aatggcggac gcgcacagac aaaccgttct cgcaggaaaa cgcccgaaaa
      601 tcgtccgtgc cggcttggtt ttgaacaggc ggcatatgcc cgcgtccgcc gccatcatac
      661 gcttccggca cggcacaggc cgccaaagac aaaaccggta cggtcagcgc gaaaaacctg
      721 atattcataa aagctcccca ataaaaataa gatatgaaac aaccgccctg attccaagct
      781 gcggcaacgc catactataa acggacgcgc aaacacacaa gcccgataac cggaatttac
      841 ccgcgatgaa tcaataatcc ggattgcgcg ccccttcttt acccgtcttc cggcaccgcc
      901 ttttgcctga cgatgccgtc caaacccgcc tgtccgcccg aaggaatgta aattttttcc
      961 aaattccaag taaaaaccgc tatcagtgtg ctaatttgcg ttaaaatcct attcggcgtt
     1021 taacgttttg tgcgcccgca tccctgcact gtttgatgcg ggcataaggc acaaatcccg
     1081 acaagcgcac tgtttcatac ttcgtcaatc attcagactc cggtttgtgc ccgtgccggc
     1141 agatggttcg gccgtttccc gccgttcagg catattccga cagtgtgaga taaggattta
     1201 ttcgatgaaa tcactcaaaa ccttcctcat ttggggcata gtggtactgg tcggcttagc
     1261 atcctttacc actctggccc tcagccgagg cgaacaggtc agcgcggtat ggatggttac
     1321 cgccgccata tccgtttatt gcatcgccta ccgtttttac agtctctaca tcgccaaata
     1381 tgtgatgcag cttgatccga accgcctgac tccggcagaa cgccacaacg acggcttgga
     1441 ctacgttccg acgcacaagg gcgtattgtt cggacaccac tttgccgcga ttgccggcgc
     1501 gggccctttg gtcggcccgg ttttggcggc gcaaatgggt tacctgcccg gtactttgtg
     1561 gattatcttc ggcgtggtgt ttgccggcgc ggtacaggat atgatggtct tgttcgtctc
     1621 tatgcgccgc gacggtaagt ctttgggcga tattgtgaaa caggaactcg gcactgtccc
     1681 cggcgtgatt gcctccatcg gtattttgat gattatggtc atcattatgg cggtgttggc
     1741 gttgattgtc gtaaaagcac tggttcacag cccttggggt acgttcacca ttgccgccac
     1801 tatgccgatt gccctgttta tgggtattta cacgcgctat atccgtccgg gcaaaatcgg
     1861 cgagatttcc atcgtcggct ttattttgct gatgctggcg gtaatttacg gcgaagatgt
     1921 ggcgaaaagt tccatcggac attggttcga ccttgacggc atccagctca cttgggcgat
     1981 tatgatttac ggctttgtcg cctccgtatt gcccgtatgg ttgctgctca ctccgcgcga
     2041 ctatctctcc accttcctga aaatcggtac gattgcggcc ttggctttgg gcatcgtcat
     2101 cgtcaatccc actttgcaaa tgcctgccgt aacccacttt atcgacggtt cgggtccggt
     2161 attctcaggc gcattgttcc cgttcctctt catcaccatc gcctgcggtg cggtttcagg
     2221 cttccacgcg ctgatttctt ccggcaccac gccgaaaatg ctggaaaacg aaacccacgt
     2281 ccgtatgatt ggttacggcg gtatgttgat ggaaagtttc gtagccatta tggcgcttgc
     2341 cgccgccgca tcgcttgatc ccggcgtgta cttcgccatg aacagccctg ccgccctgat
     2401 cggtacggat gccaacaccg ccgccgaagt gattaccacc aagctgcaat tccctgtcga
     2461 tgccgcaacg ctgttgcaca ccgctaaaga agttggagaa aacaccatcc tgtcccgtgc
     2521 cggcggtgcg cccaccctcg cagtcggtat ggcgcacatt atgagccgcc tgattccggg
     2581 cgaggcgatg atggcgttct ggtatcactt cgccctgttg tttgaagcct tgttcatcct
     2641 gaccgccgtc gatgccggta cgcgcgtcgc acgttttatg attcaagact tgggcagcat
     2701 cttctacaaa cctttcggca acaccgactc catcccagcc aacctgattg cgaccttctt
     2761 cgccgtggca ttgtggggct actttctcta caccggcgta accgacccgc tgggcggcat
     2821 caactcgctc tggcctttgt tcggcatcgc caaccaaatg ctggcgggcg tggccttgat
     2881 tatgtgtgcc gtggtgctga ttaagatgaa acgcgaccgt tatgtttggg tggtactcgt
     2941 tcccgccgtc ggcgtactgt tcgtaacctg ctacgccggc ctgcaaaaac tgttccacag
     3001 cgatccgcgc atcagcttcc ttgcccacgc cggcaaatac agcgacgcat tggctaaaaa
     3061 cgaagtcctt gcgcctgcca aagacatcgg cgaaatggcg caaatcatct tcaacgacaa
     3121 gattaatgcc ggtctgaccc tcctcttctt gtcggttgtc gtgattgtcg ccgcgtacgg
     3181 tttgcgtacc gccctcaaag cacgcaaagt cggctggccg accgccaaag aaatcccggc
     3241 ggtgtaccgc gacggcaaac agccggaggc acaaagtgaa gcataagctc gcgtcttggt
     3301 ggaaaaccat caagctgacg gcaaacttga tggcaggcgt gcccgattat gaaaactacg
     3361 ttgcacagca gcgcaaacat aatcccaacg cccccgtgat gaccaagctg cagtttcaag
     3421 actattgccg caaacgccgc tgcggcgcaa acggcggacg ctgctgttag ccccgcctga
     3481 aacaaatgcc gtctgaacgc cgcttcagac ggaattttta taatatagtg gattaacaaa
     3541 aatcaggaca aggcgacgaa gccacagaca gtacaaatag tacgaaaccg attcacttgg
     3601 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg acgtaccggt
     3661 ttttgttaat ccgctatacc acgatgaatc cttcgcaata tctgtttatc gacctcaatt
     3721 ttgacaaaat accggatacg cgcctttgtt gcttttccat cttccaacca actgtaaatc
     3781 tcaaacagcc ggtacacgcc atgcttcagt ttcttttcct gtcggcggat tgtttcgaca
     3841 aagaattgaa aatccatttc atgcacctta aaatttaatc tgcattcaaa ccttttcact
     3901 ttggaagcac catttatcgg atgtcccttc gcaataaaca aattttcccg ataccgccgc
     3961 ccatttcaac ccaaacccaa aacctatgaa aaacctcatc gccttcaaca aaccctatgg
     4021 cgttatctgc caattttcac cgcacgaaaa acacaaaagc ctcaaagact ttatcaatct
     4081 tcccggcttc taccccgccg gacggctcga caccgacagc gaggggctgc tgctgctgac
     4141 cgacgacggc aggcttcagg cacaaattac cgaccccaaa ttcaaacacc ctaaaaccta
     4201 ctgggcgcaa ctggagggcg tacccgacga aagccgattg gaaagcctaa gaaaagggat
     4261 agacttaggc ggtttcgtta cccgtccggc aagcatccgc atcttgaaac acggagaagc
     4321 agattcgtta tgggagcgca tcccgccgat acgcgtccgc aaaaccgttc ccgatttttg
     4381 gattgaaatt accatttctg agggcaaaaa ccgccaagtc aggcgaatga ccgccaaggc
     4441 gggctatccc tgcctgcgtc tgatcagagt ggcaagcggc agactgaaac tgtttgattt
     4501 ggatttaaaa cccggggaat gggcatacgc cccgtttaaa ccataatcac gtttatctca
     4561 tcatttccac aaaagtggga atccggaatt ttatagtgga ttaacaaaaa tcaggacaag
     4621 gcgacgaagc cgcagacagt acaaatagta cgaaaccgat tcactcggtg cttcagcacc
     4681 ttagacaatc gttctctttg agctaaggcg aggcaa
//
LOCUS       DAXIEB010000126         4712 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000126 DAXIEB010000000
VERSION     DAXIEB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.126"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(27..167)
                     /locus_tag="ACFOA3_001822"
     CDS             complement(27..167)
                     /locus_tag="ACFOA3_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898779.1"
                     /translation="MPSENLSDGIFYQSSHIFYQGCKIYPKQQKPPCRHSRKSGNLEH
                     KI"
     gene            complement(185..1123)
                     /locus_tag="ACFOA3_001823"
     CDS             complement(185..1123)
                     /locus_tag="ACFOA3_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6898780.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1132..2007)
                     /locus_tag="ACFOA3_001824"
     CDS             complement(1132..2007)
                     /locus_tag="ACFOA3_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6898781.1"
                     /translation="MPTVHFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGRRLQHLARGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            complement(2203..2279)
                     /locus_tag="ACFOA3_001825"
     tRNA            complement(2203..2279)
                     /locus_tag="ACFOA3_001825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2243..2245),aa:Met,seq:cat)
     regulatory      2429..2528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2619..4520
                     /gene="thiC"
                     /locus_tag="ACFOA3_001826"
     CDS             2619..4520
                     /gene="thiC"
                     /locus_tag="ACFOA3_001826"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6898782.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKHGLPHIRTAWLDE
                     RGDTEILPKLSSEYGTERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1148 a         1348 c         1242 g          974 t
ORIGIN      
        1 gaaacttgtc gggaaaaacg gtttctttag attttatgtt ctagattccc gcttttgcgg
       61 gaatgacggc agggtggttt ctgttgtttc ggataaattt tgcagccctg ataaaaaata
      121 tggctgcttt ggtaaaaaat gccgtctgaa aggttttcag acggcatttt gtttttaaga
      181 agcatcagcg gaagcggacg atttccctgt cttcgatatg gatgcgtacc gtatcgtttc
      241 ccgatatttc accggcgtgc ggcatatcga ggttcagcca caggatgccg tgttccggat
      301 ggaggacgga caggctgaac gattcgggca aacaggtacg ggataatacg cggcactcca
      361 tgccgtcttg gtcgaaacgc accgcatttt gcggaatatg gcggtcatcg tcggtattgg
      421 gcaaacccat cagtcgggcg acctgcacgc cggcaggcgt ttgaaccaag gtttcgggcg
      481 taccgcattg aaggattttc ccctcgtgca tgacggcgat ttcgtccgcc gccgtgcagg
      541 cctcttcggg cgaatgcgtt accaaaacgg cagggatgcc gccgtttcgg atacgttcgg
      601 cagtcatgcg gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac gattcgtcca
      661 gcaacagcaa ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt tgcttctcgc
      721 ctccggaaag tttttcaggc ttgcggtgcg cctcattttc cagtccgact tcggcaagtg
      781 ccgccatggc aaggcgttcg gcttcggctt tcggcatttt ttgcattttc aaaccgaatg
      841 ccgcattttc cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaaa
      901 tacggcgttt ttcgggcggc atacgggtaa tgttttcccc gttcagccga atttcgccgc
      961 cgtccggacg gataatcccc gcaatcatat tcagcagtgt tgattttccg cagcccgacc
     1021 gccccaaaac ggcgagtatt ttgccgcgcc cgacagtcag gcagatatcg tcggcaaccg
     1081 ttttgccgcc gaagcgtttg cagagtccgt tcagttcaag catggcgcat cctataaacg
     1141 tatgccgtcc agccactcgg acagcggatg ggcgacatca atcagaccgt aacggcaaaa
     1201 cgcgtccagc gtaaccagtt gcgcgtcgtg catcatgttt tccgacaaca tggcggcaac
     1261 caggctgccg atgtccattt tctcaaaaca cgcaacctcg ccatcctgat tttcaggcag
     1321 gaaggtttcg ggcaggacgg catcgaacac atacaggatt tcattgtgca caccccggct
     1381 gacggggcgc aggctgtgca gccgcgatac cgggcggatg agcggaaaca gcgttttatc
     1441 caaaccggct tcttcgctgc tttcgcggca cacggcttca gacggcattt cgccgccgga
     1501 aacaccgccg gcggcaatat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg
     1561 cttgcctatc cagaaatgcc atcggccgtc cgattcggtc agtccgttga gatggacggc
     1621 gcggctgagc agtccgaacg gacggaaagc ggcgcgttcg agcgtgaaca aggggtttcc
     1681 gccgccgtcg gtcaggtcga aacactcgtt gcgccagccg tccaacagtc ccgcacagtg
     1741 ccaaccgcga gcgaggtgct gcaagcgtct gcccatatct ggccagccgt ccgcattcag
     1801 gaaaatgccg tctgaagact ccgagcagcc tgcctcccag tcttttttga tgcgctccgc
     1861 ccattccggc gacaggttac ccaaaggcag accgttcaga tacagcgttt tccagcaact
     1921 ttctgcaccg taacttgctt ttgcccactc gaacagggca tcaaggtctt gtttgctgac
     1981 ggattcggta aaatggacgg tcggcatagt cggctttctt gaacacaatg cggaagattg
     2041 tagccaaacc gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc
     2101 ggcaggaggg aaaatccggc aaaaaacgga ggcggcagga aacaggaaac aggaaacagg
     2161 caaaacaaaa cccctcggca ttgaaccgag gggtttgata tttggttgcg ggagcaggat
     2221 tcgaacctac gaccttcggg ttatgagccc gacgagctac catgctgctc catcccgcgt
     2281 cagaagatga aactatacgg cagatttttt atgttgtcaa acattatttc cgcgaaaaat
     2341 cataaaattt tgccgtccgg cggtttatgc ctgatttgaa atattatttt ctttacaaaa
     2401 gttcatgttc gtgatttaat tttggttaac attgaaacag gggtgctgcc tgatgtttag
     2461 gcggctgaga aatacccttt acacccgatc gggataatac ctgcgtgggg agttttcacg
     2521 gattctgctt ttcagacggc attggttttc aaatgccgtc tgaaaacgca aaacgctcct
     2581 gtttctttat tctaaacgag aaaacaggag cattttttat gactacgcca aaaaaaaccg
     2641 ccaaaacttc cggcaacgaa gcgcgcgagc ttgccgactt gagcgaagac atcggcatcc
     2701 gctttaaata tccgaactcg gagcgcgtgt atctgcaagg cagccgcgac gacatccgcg
     2761 tgcctttgcg cgaaatccgt caggacgaca cctacacggc gcaaggcacg gaagccaacc
     2821 cgccgattcc cgtctatgac accagcggcg tgtacggcga tccggcggcg cacatcgacc
     2881 tgaaacacgg tttgccgcac atccgcacgg cgtggctgga cgaacgcggc gataccgaaa
     2941 tcctgcccaa gctctccagc gaatacggca ccgaacgcgc acacgatccg aaaaccgccc
     3001 atctgcgctt caaccaaatc acccgcccgc gccgcgcgaa aagcggcagc aacgtaaccc
     3061 agcttcacta cgcgcgccaa ggcattatca cgcccgaaat ggagtttgtc gccatacgcg
     3121 aacgtttaaa attagacgaa ttgtcccaaa aaccggaata cgccaagctc ttgaaacaac
     3181 acgcagggca aagtttcggc gcgaatatcc cgacccatcc cgaccaaatc acgcctgaat
     3241 tcgtgcgccg agaaatcgcc gccggacgcg cgattattcc cgccaacatc aaccaccccg
     3301 aactcgaacc gatgattatc ggccgcaact tccgcgtcaa aatcaacggc aacttgggca
     3361 actccgccgt aacctccagc ctgaccgaag aagtcgaaaa aatggtgtgg tcgctgcgtt
     3421 ggggtgcgga cacgattatg gatttgtcca ccggcgcgca catccacgaa acacgcgaat
     3481 ggattatccg caacgcgccc gtccccatcg gcaccgtgcc gatttaccaa gcgttggaaa
     3541 aaaccggcgg catcgccgaa gatttgactt gggatttgtt ccgcgacacc ctcatcgaac
     3601 aggcggagca aggcgtggac tatttcacca tacacgcggg cgtgttgctg cgttatgtgc
     3661 cgatgaccgc caaccgcctc accggcatcg tctcgcgcgg cggttcgatt atggcgaaat
     3721 ggtgtttggc acatcatcgg gaaaatttcc tctacacgca tttcgacgaa atctgcgaaa
     3781 ttatgaaagc gtatgacgta tcattcagcc tcggcgacgg cctgcgcccc ggctgcattg
     3841 ccgatgccaa cgacgaatcc cagttcgccg aattgcacac cttgggcgaa ttgaccgata
     3901 aagcgtggaa acacgacgta caagtcatga tcgaaggccc cgggcatgta ccgctgcaac
     3961 gcgtcaaaga aaacatgacc gaagagctgc aacactgctt tgaagcacct ttttacacgc
     4021 tcggtccgct cgttaccgac atcgcacccg gctacgacca catcacctcg ggcataggcg
     4081 cggccaatat cggctggtac ggcaccgcca tgctctgcta cgtgaccccg aaagagcatc
     4141 tcggcctgcc cgacaaagaa gacgtgcgca ccggcatcat tacctacaaa ctcgccgctc
     4201 acgccgccga cctcgccaaa ggctggccgg gtgcgcaatt acgcgacaac gccctgagca
     4261 aggcgcgttt cgaattccgc tggcgcgacc aattccgctt aagcctcgac cccgaacgtg
     4321 ccgagagctt ccacgacgaa accctgcctg ccgaaggcgc gaaaatcgcc cacttctgct
     4381 cgatgtgcgg ccccaaattc tgctcgatga aaatcacgca ggaagtgcgc gactacgccg
     4441 acaagcaaaa agcccaacgg cagggaatgg aggaaaaagc ggtcgagttc gtcaaaaaag
     4501 gcgcgaagat ttacagttga aacgtcaagc aaaaaatgcc gtctgaaacc cggaaaaaag
     4561 gcttcagacg gcattctttc gcttgtgaaa atatagtgga ttaacaaaaa tcaggacaag
     4621 gcgacgaagc cgcagacagt acagatagta cggaaccgat tcacttggtg cttcagcacc
     4681 ttagagaatc gttctctttg agctaaggcg ag
//
LOCUS       DAXIEB010000127         4703 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000127 DAXIEB010000000
VERSION     DAXIEB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.127"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(420..956)
                     /locus_tag="ACFOA3_001827"
     CDS             complement(420..956)
                     /locus_tag="ACFOA3_001827"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6898783.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(990..1346)
                     /gene="folB"
                     /locus_tag="ACFOA3_001828"
     CDS             complement(990..1346)
                     /gene="folB"
                     /locus_tag="ACFOA3_001828"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6898784.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1402..2004
                     /gene="plsY"
                     /locus_tag="ACFOA3_001829"
     CDS             1402..2004
                     /gene="plsY"
                     /locus_tag="ACFOA3_001829"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6898785.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLIAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            complement(2023..2148)
                     /locus_tag="ACFOA3_001830"
     CDS             complement(2023..2148)
                     /locus_tag="ACFOA3_001830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898786.1"
                     /translation="MFEHIGVVAGVECVSVTEHGMKAVCAEMPSERGNMVSDGMT"
     gene            2092..2937
                     /locus_tag="ACFOA3_001831"
     CDS             2092..2937
                     /locus_tag="ACFOA3_001831"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6898787.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNKKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3183..4157
                     /locus_tag="ACFOA3_001832"
     CDS             3183..4157
                     /locus_tag="ACFOA3_001832"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6898788.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLSAGYVLYGPQTQ
                     LVFTLGHGVYVFTLNTENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAHLDRLHAK"
     gene            complement(4300..4518)
                     /locus_tag="ACFOA3_001833"
     CDS             complement(4300..4518)
                     /locus_tag="ACFOA3_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6898789.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1208 a         1406 c          985 g         1104 t
ORIGIN      
        1 cgttctcaaa ttcaaattgg gcttttaggt ctataacaaa gaaaaagcct tggtaggata
       61 aacctaccaa ggctttttct taaaaaaacg ctctattacc gtgcctaaaa atgcaaaaaa
      121 gtaaccgtca taaccctttt gccaaaatca atatcatcat ccgatgaatg aattgacggt
      181 atataagaaa gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagatattc
      241 attattaacc attttaaaaa ttatcatgtt cgtttgggta attattatac ccttttccct
      301 cttattacta aagcacgaat gactttaaag gacgcgccca gagacaagcc gaaaaggcat
      361 atctcatccg ccaaacaaaa tgcccaccga cttactccgg caggcagttc aacaccctgt
      421 caatccttca tcagccagta ttgcaaaccg attaatgtct tgccgtcttt aatttcatcg
      481 tttgctaatg cctgacggac ttcttctttc gacatcaata cggtttccgt aatctcgtct
      541 tcgtcattgg caagcgtact gcccaaacgc acgccttccg cttcgaacag atacattttt
      601 tcgttgcaaa aaccgaccgc cgtataaaaa ctgtaaagca ggcgcacgct gtcggcaaca
      661 taaggcgttt cttcagccag ttctctcagc gcacacgctg ccatatcctc gcccgccaca
      721 tccagcttgc ccgcaggaag ttccaatgtc gcctgatttg ccgcataccg ccactgccgt
      781 accaaaacga ccttttcatc atccgttacc gccaacacgc acgccgctcc cggatgacgg
      841 atgacgatac gctgcccttc attgccgttg ggcaacctga ccttatccct gctgatgctg
      901 acaaaaccgc cctcgtaaat ggtttcgccg cctaatttta cttccctcaa atccacttca
      961 tatctccttt ccgtttccta ttctgccgtt caatcttcac gcttgccgcg ctcgatttcc
     1021 acgccaacct cgcgcacgcc ttccaaaata cccggcttga cgattttcac gcgtacccac
     1081 accgcgccga aatatcccaa aaccaaatcg gcaatatatt ccgccaacgc ttccaaaagc
     1141 aggaaatcct gttctttcag atgtcggcgc agtgtttcgc atacctcggc ataatgtacc
     1201 gtattggcaa tatcgtcgtc cgaacccgct ttctcgggaa cgccgatgtc caaatcgaca
     1261 atcagggtct gcaaccgttc gcgttcccag ccgtacacgc cgataagcgt atccgccttg
     1321 atgccgtgca aaaagatttt atccataatc cgccccgttt tttgctaaaa taaccgcacc
     1381 attctaagta aagtaccaca aatgttcaac ataccggctg ttgccgtttc ctatctgatc
     1441 ggctcacttt ctttcgccgt catcgtttcc aagtattacg ggatggacga cccgcgcacc
     1501 tacggatcgg gcaatcccgg cgcgaccaat gttttacgca gcggcaaaaa aaaggcagcc
     1561 gcgctgacgc tcttgggcga tgccgccaaa ggtttgattg ccgttttgct tgcacgcgtg
     1621 cttcaagaac cgctcggttt atccgacagc gcaatcgctg ccgtcgcact cgccgcgctg
     1681 gtcgggcata tgtggccggt gtttttcgga tttaagggcg gcaaaggcgt ggcaacggca
     1741 ttgggcgtgc ttttggcact ctctcccgca actgccttgg tctgcgcgtt gatttggctt
     1801 gttatggcat tcggcttcaa ggtgtcctcc cttgccgcat taaccgccac aatcgccgcc
     1861 ccccttgctg cgctgttttt tatgccccac ctttcttgga tttgggcgac gctccttatc
     1921 gccctgctgg tgttgttccg ccacaaaagc aatatcagca acctgcttaa aggcaaagaa
     1981 ggcaaaatcg gcgaaaaacg ctgattcggc atttgttttc cattacgtca tgccgtctga
     2041 aaccatattt ccgcgttcag acggcatttc cgcacaaacg gctttcatac catgctcagt
     2101 tacagacacg cattccacgc cggcaaccac gccgatatgc tcaaacattt caccttgttt
     2161 ttagtgctgc aatatttcaa caaaaaagac aagccctact ggtacatcga cacgcacggc
     2221 ggcgcgggtt tgtacaatct cgaaggcagc gaggcacaaa aagtcggcga ataccggcaa
     2281 ggcatcgccc tgctccgaca ggcgcaaaac ctgcctgccg aactctctga ctttgccgcg
     2341 cacatacaaa aaatcctgcc ctcgcccgaa ctttactgcg gttctccgtg gctggcgcaa
     2401 tcgctgaccc gcgtcggcga caaattacgc ctgtttgagc tgcaccccac cgactttgtc
     2461 catctgcaaa acaatatggg cgaagcagga ttgggcaaac gcggacaagt gttgcgcgaa
     2521 gacggttaca aagagctgat ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc
     2581 atcgacccgc cctatgagga aaaacaggac taccggcgtg taacggaaac gctgaaagcc
     2641 gctttaaaac ggtttgagtc cggctgttat ctcatttggt atccctgcct cagccgcgaa
     2701 gaaagccgca agctgcccga agaattgaaa aaactcctgc ccgacaacta ccttcacgcc
     2761 gaacttcacg tccacgcgcc gaaagccgac ggtttcggta tgcacggcag cggaatgttc
     2821 gtcatcaatc cgccctatct gttggcggaa caattggcgg caaacctccc cgccctgacg
     2881 cggctgctgg cgcaagacga aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc
     2941 cgatgccgtc tgaaaccctt cagacaacat ctcccgtcaa ataaacaaaa ccggacgata
     3001 taccggcagt cggcactttt aagaataaat atcccctcat ccgcctgccg cgttccgact
     3061 tgccccatgc cgttttttta cactacaatc ccctagacta tcctgcaaaa cgcaccaaag
     3121 gaatacgcca caataacctg ccgcctttca gacggcacaa acctttccga aacgagcaaa
     3181 ctatggacac actgacccga ttcctccccg aacacctgca acaaaaccag ctgcccgaag
     3241 cactcggcgg cgttttactg tccgttgttt ccgcctgcac cgaaatcaac gccaaagtcc
     3301 gcctcggcgc actggccggc gtattgggta tggcgggcac gggcaatatt cagggcgaag
     3361 accaaaaaaa actggatgtt atcgccaaca acatcatgat tgacacactc aaagccaacc
     3421 ctgccgttgc cggtttggca agcgaggaag aagactcttt cgtaagtgcc ggtgaaaacg
     3481 gacgctatct cgtcctattc gaccctttag acggatcgtc caatattgat gtcaacattt
     3541 ccgtcggtac gattttctcc atccttgaaa aacccgaagg cgcattggca accgaatcat
     3601 tccttcaaac gggcagacag cagctttccg caggctatgt cctctacggg cctcaaaccc
     3661 agctcgtatt cacactcgga cacggcgtgt acgtgtttac actcaatacc gaaaacgaat
     3721 ttgtcctgac caaagaaaac ccgaaagtac ccgaaagtac caaagaattc gccatcaata
     3781 tgtccaaccg ccgccactgg ctgccccccg tccaacaata catagacgag ctcttggcgg
     3841 gcgaaactgg cacgcgcggc aaaaactaca atatgcgctg ggtagccagc atggttgccg
     3901 aaatccaccg catcctgatg cgcggcggcg tgttcatgta tccgcaagac aaacgcgacc
     3961 ccgccaagcc cggcaaactg cgcctgatgt acgaagccaa ccctatgagc ctgatactcg
     4021 aacaggcggg cgcatcggca agcaacgcat atcaagccat gctcgacata cagcccgaaa
     4081 acctgcacca acgcgtcgcc gtcttcatgg gcagcagcga agaagtcgcc catctcgacc
     4141 gcctgcacgc aaaatagaca gacagccgtt cacacaagta cacggcggcg gcaatccgat
     4201 accgccgccg ttatttcaaa cagcacaagc cttccggtca aatgacaaaa atgccgtctg
     4261 aaaacatttc agacggcatt tcaaaaactc aaacccgaat tattttcccg ctgctttttg
     4321 caggatggcg tacaactcgt ctttcagttt caatttggct tttttcagct catcgatggt
     4381 ttccgcaccg ctggtaaccg gattgttgac cagaccggta attttatcgt ccaactcgtt
     4441 gtgttcgtcg aacagacggg cgaagcggga attttcctgt ttcaatttgg aaatcaaatc
     4501 acggtattct gggaacatat cgctttcctc tcttggttta cggttaataa agtcaaactg
     4561 aatctttaca ttaattataa caaagataat atttttatcc agtattttat cggatacggc
     4621 aaaattttct tttaacaccg ccgtccggtt gataaaaacg ttgacaatat cacgctttac
     4681 gcataaattt tcagcctttc cta
//
LOCUS       DAXIEB010000128         4683 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000128 DAXIEB010000000
VERSION     DAXIEB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.128"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(269..682)
                     /locus_tag="ACFOA3_001834"
     CDS             complement(269..682)
                     /locus_tag="ACFOA3_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898790.1"
                     /translation="MKKLDDKEQAMYIAKKFQKRMLIPKIFFISVACISGAATLQEIV
                     TNNNFLKKDIFFIIFTIYWVVGLLFCIIYIPIASICPYCHSFQKFNGKSFEIDANNLT
                     YSRGISPFNKDYCTECKAPLSPKAVEYMYSKIADE"
     gene            complement(666..2057)
                     /locus_tag="ACFOA3_001835"
     CDS             complement(666..2057)
                     /locus_tag="ACFOA3_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6898791.1"
                     /translation="MKDEIIGNLDNYKYKQAIACMNSKGRLKAQNDGSYDAYAKLNET
                     ERQKILINYSETYRKADAEAQNWGVGGKHSRALNAGITLATGLLGGQSGLQSAVNAAA
                     PYASEAIGRTFGHGENKNETAQAVGHFLLGAAIARVNGGNFAAGGSAAVAAEKAAEHL
                     AQRYNDGKTAIDPQTGEFNANLLPEHIKEEIKSKSGVIASLTGAAVGGTPVDAQTGGA
                     VGQNAVENNHLLAGQRIQKNAEFRACKADWRCEFEVDKKWEKIGLNNMRKLHAACDKG
                     INTAACQNLRNQIDRSTYQGKRDYNYPTGLELKGELSGALGVGVQANAKVTVTLGNRG
                     SSVQAEGGVGLGIGAALSGGLSKQTRHVGIDENKTLNVSTEIVLGEKQFGTQADNNNA
                     TLSTKVEAELKLGPLNHSAAIQGGRQYPDNQVSSLYRGGEVKSVVKPQLGIGGMLKWD
                     IWNGRSKRYEKIR"
     gene            complement(2020..2646)
                     /locus_tag="ACFOA3_001836"
     CDS             complement(2020..2646)
                     /locus_tag="ACFOA3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898792.1"
                     /translation="MEKIEKFKSELLNDIFQYIQCISIFVHKKKIYYLIDYKENFELN
                     AKISFDIYLREGIITKEQYNYDYKNYRNGIWQLTKDNFESYLQSDSVIVLKKDELKEL
                     MFQGFTSAEAVGLYSVVENKLSYNDPISDSGQQNDFLKINQISSRLPLFYVNFDTEVY
                     LHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(2777..3271)
                     /locus_tag="ACFOA3_001837"
     CDS             complement(2777..3271)
                     /locus_tag="ACFOA3_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HFC6898793.1"
                     /translation="MTFKQEQDYWAGYKANERALIIQTWSGFGRYAPDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSSEDQDFFDTEKFRQRYEDWVAKLCGNLGYKTRR
                     ALFKNMMSGAIWLHNGRLKISPSRHVKLEAWDAIDADDVILSLDNSPEEIGAGLKLAL
                     SRCR"
     gene            complement(3272..>4683)
                     /locus_tag="ACFOA3_001838"
     CDS             complement(3272..>4683)
                     /locus_tag="ACFOA3_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6898794.1"
                     /translation="AEKAAIRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGL
                     TTGSLGGILAGGGTSLAAPYLDKAAENLGPAGKAAVNALGGAAIGYAAGGNVGTAAVG
                     ANVDWNNRQLHPKETQILNKLSKGKSAEEQYRLKAAACALTQCAEGVPDFDPLYKGLK
                     NLQDASKQFVAEQNVLMRTGAFKYGTWNSLNDIRSSYDRAATKIKGAGNMGLGATTFV
                     GSGAVGGGLCSTGIGCAAGGLIATAGMTGGYTQASEGSRQLFGTYQSDFGRKVVLSLG
                     TPIEYESPLVSDAKNLALWGLQTLITRELGNLATGVKTSLTPKTADVQRNILSQSEVG
                     IKWGKGIEGQGMPWEDYVGKGLSANARLPKNFKTFDYFDRGTGTAISAKTLDTQTTAR
                     LSKPEQLYSTMKGYIDKTVNFKSYELSGVPLRADMIKQREIHLAIPAQTNKEQRLQLQ
                     RVVEYGKNLNITVKITEIE"
BASE COUNT         1127 a         1151 c          928 g         1477 t
ORIGIN      
        1 ggcggtttcg tttttgtttt caccgtggcc gaatgttctg cctattgctt cggaggcata
       61 aggcgcagcg gcattggctg cgctttgcaa cctcgctggc tcggtgacgt taaacaaaag
      121 ccgtctgaaa cggtttttct gttttcagac ggcctcttag ctcaaacctt gagaaccgca
      181 tacgtgtgtt ccatgcacat cctacttgtt gagtttagat ttcatatggt ctatggcttg
      241 ctaattgcac aggctacgcc tgttatactt actcatcagc tattttggaa tacatatact
      301 ctacggcttt aggtgatagt ggtgctttac actcggtgca ataatcttta ttaaatggag
      361 aaattcctct tgaataagtt aaattattag catcaatctc aaatgatttg ccattaaatt
      421 tttgaaaaga atggcaatat ggacatatgc tggcaatcgg aatataaata atacaaaaaa
      481 gtaatccaac aacccaatat atagtaaata taataaaaaa aatatctttc tttaagaaat
      541 tattgtttgt aacaatttct tggagggttg ccgcaccaga tatacaggct acggaaataa
      601 aaaaaatctt aggaattaac attctttttt gaaatttttt cgcaatatac attgcttgtt
      661 ctttgtcatc taattttttc atatctttta cttcttccgt tccaaatatc ccatttcaac
      721 ataccaccta ttcccaattg aggtttaaca acagatttaa cttcccctcc tctatacaat
      781 gaagatactt gattatctgg atactgtcta cctccttgta tggcagcaga atgattcaac
      841 ggccctaact taagttcagc ttcaacttta gttgacagtg tcgcattgtt attgtcggct
      901 tgcgtaccaa attgcttttc tcctaaaact atttctgttg aaacatttaa agtcttattc
      961 tcatcaatac ctacatgtct agtttgtttt gataatcctc ctgataatgc tgctccaata
     1021 cctaaaccca caccgccttc agcttgtacg gacgaacctc tgttccctaa agttacggtt
     1081 acttttgcgt tggcctgtac acctaccccc agcgcgcctg acagctctcc tttcaattcc
     1141 aagcccgtgg ggtaattata gtcacgttta ccctgatatg tagatctatc aatctgattc
     1201 ctcaaatttt ggcatgcggc agtattgatc cctttatcac atgccgcatg aagttttctc
     1261 atattattaa gcccaatctt ctcccatttt ttatcaactt caaattcgca acgccaatcg
     1321 gctttgcatg ctcggaattc cgcattcttt tggattcgct gccctgcgag cagatggttg
     1381 ttttccaccg cattctgccc gaccgcccct ccggtttgcg catctaccgg cgtgccgccc
     1441 acggccgctc ccgtcagcga tgcaatcacc ccgctcttcg atttgatttc ttctttgata
     1501 tgctccggca gcaggttggc attgaactcg cctgtttgcg gatcgattgc ggttttacca
     1561 tcgttatacc gttgggcaag atgttccgct gctttttcag ctgcaactgc tgccgagccg
     1621 ccggcagcaa aattaccacc gttgacgcgg gcaatagctg ctcctaaaag gaaatgtccg
     1681 acggcttggg cggtttcatt tttgttttca ccgtgaccga atgtcctgcc tattgcttcg
     1741 gaggcataag gcgcagcggc attgactgcg ctttgcaatc cgctttgacc accaagtaga
     1801 ccggtcgcga gggtgatgcc ggcattaagg gcacggctgt gtttacctcc caccccccaa
     1861 ttttgggctt cggcatcggc tttgcgatag gtttctgagt agttgattag aattttttgc
     1921 cgttcggttt cgttcagttt ggcgtaggca tcatagctac cgtcattttg ggctttcaga
     1981 cggccttttg aattcataca agctatggct tgcttatatt tataattgtc taaatttcca
     2041 ataatctcgt ccttcaattt tccaataaca aaattcgtct ggtattaaat acccaaaatc
     2101 catcgccttt gaaaaccagc cgtcataaac ataatcttca tgacatctgt cccaatccat
     2161 atgtaagtac acttcagtgt caaaattgac atagaataaa ggcagcctcg aggaaatctg
     2221 attgattttt aagaaatcat tttgctggcc ggagtctgag ataggatcat tgtacgacag
     2281 cttgttctca acaacggaat ataaccctac cgcttcagca gaagtaaacc cctgaaacat
     2341 cagttccttc aattcatcct tttttaatac aatgactgaa tctgattgaa gatagctttc
     2401 aaaattgtcc ttagttaact gccagatacc attacggtaa tttttataat cataattata
     2461 ttgttctttt gttataattc cttctcttag ataaatatca aaagatattt ttgcgtttaa
     2521 ctcaaaattt tccttataat caatcaaata atatatcttt ttcttgtgga caaaaataga
     2581 tatgcattga atatactgaa agatgtcatt taatagttca gatttaaatt tctcaatctt
     2641 ttccatattt gccaaattcc ttaataactt tatcttcagc aagccgtagc ccgcattaga
     2701 cctatggcgg gctttgagtg tgataaaaaa gatcgtctga aacaagtttc cggctttcag
     2761 acgacctttt gtaatattat cggcagcggc tcaatgccaa ctttaaacct gctccgattt
     2821 cttcagggct gttatccaat gataaaatga catcgtctgc atcaatggca tcccacgctt
     2881 ccagcttgac atggcggctc gggctgattt tcaagcggcc gttgtgcagc caaatagccc
     2941 cgctcatcat gtttttaaat agggcgcgtc tggttttata gcccaagttc ccgcatagct
     3001 tggcaaccca atcctcatag cgttgccgaa atttttcggt atcaaaaaaa tcttggtctt
     3061 ccgaactgtc ataaacgaaa gtcctgctgt ttgccaacgc ttgcaagacc gttgtgccta
     3121 aagtttcatt gtcggtatcc aatggcagga tatggggggg atataggtgg tctggagcat
     3181 atctcccaaa tcctgaccat gtttgaataa tcaaggctct ttcatttgcc ttatagccag
     3241 cccaataatc ttgttcttgt ttgaaagtca tttattcgat ctccgtaatt ttgactgtaa
     3301 tgtttagatt tttgccatac tctaccacgc gttgcaactg caatctttgc tccttattag
     3361 tttgtgcggg tatggccaga tggatttcgc gctgtttgat catgtcggcc cttaacggta
     3421 ctcctgataa ttcataactt ttgaaattta ccgtcttatc gatgtaccct ttcatggtgc
     3481 tgtaaagctg ttcgggtttg gacaggcgtg ccgtagtttg cgtatccaga gttttggcac
     3541 tgattgccgt gcctgtacca cgatcaaaat aatcaaatgt tttaaaattt ttaggtaacc
     3601 ttgcattggc agacaagccc ttaccgacat aatcctccca aggcattccc tgtccttcaa
     3661 tccccttgcc ccacttgata ccgacttcgg attgggacag gatatttcgc tgtacgtcag
     3721 cagttttcgg agtcaaggaa gttttcaccc ccgttgccaa gtttcccaat tcgcgcgtaa
     3781 tcagcgtttg caatccccat aaggctagat ttttcgcatc agatactaac ggtgattcgt
     3841 attctattgg tgtacccaaa gataggacaa cttttctacc gaaatcggac tggtaagtac
     3901 caaacaattg ccggcttcct tctgaggcct gtgtataacc accggtcata cctgccgttg
     3961 caataagtcc accggccgca cagccaatcc cggtactgca cagacctccg cccacagcac
     4021 ccgaaccgac aaaagtcgtt gcacccaatc ccatattgcc cgccccctta attttggtgg
     4081 cagctcggtc gtaactgctg cgtatatcat tcaggctgtt ccatgtccca tatttaaatg
     4141 cacccgtccg catcaatacg ttttgttccg ctacaaactg tttactggca tcttggaggt
     4201 ttttcagtcc tttataaaga ggatcgaagt caggtacgcc ttcagcgcac tgggttaatg
     4261 cacatgcagc ggcttttagg cggtactgtt cttcagccga tttgcctttt gacagtttgt
     4321 taaggatttg tgtttctttc ggatgcagct gcctattgtt ccaatcgaca ttcgccccta
     4381 ctgccgccgt gccgacattc ccgcccgccg catagccgat tgccgcccca cccagtgcgt
     4441 tgaccgccgc tttgcccgcc ggaccgaggt tttccgccgc tttgtccaaa tacggtgcgg
     4501 caagggaagt gccgccgccg gccagtatgc cgccgaggct gccggtcgtc agtccgcctg
     4561 ccgccccgtg cagtatgctc ctgcctatgc cgccttcttt ccaggtgtcg tagcggcttt
     4621 ggttttcggc aaggtaggcg tttacttggc cgagggatgc gcggatggcg gctttttcgg
     4681 ctt
//
LOCUS       DAXIEB010000129         4521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000129 DAXIEB010000000
VERSION     DAXIEB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.129"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(25..246)
                     /locus_tag="ACFOA3_001839"
                     /pseudo
     CDS             complement(25..246)
                     /locus_tag="ACFOA3_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(245..352)
                     /locus_tag="ACFOA3_001840"
                     /pseudo
     CDS             complement(245..352)
                     /locus_tag="ACFOA3_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFOA3_001841"
     CDS             complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFOA3_001841"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6898795.1"
                     /translation="MLLGVNIDHIATVRNARGTIYPSPVEAALIAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1179..1922)
                     /gene="recO"
                     /locus_tag="ACFOA3_001842"
     CDS             complement(1179..1922)
                     /gene="recO"
                     /locus_tag="ACFOA3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6898796.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(1956..3083)
                     /gene="pheA"
                     /locus_tag="ACFOA3_001843"
     CDS             complement(1956..3083)
                     /gene="pheA"
                     /locus_tag="ACFOA3_001843"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6898797.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3122..4354)
                     /locus_tag="ACFOA3_001844"
     CDS             complement(3122..4354)
                     /locus_tag="ACFOA3_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6898798.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1018 a         1294 c         1231 g          978 t
ORIGIN      
        1 ctatattaca taatcatcgc ctcaatattt aatatcccaa aatatagatt taaaaccatc
       61 gcctttgtgg tttgtcagca ttcctaatta agatagaaag tcaaataatt tcttgtttct
      121 ccggctgcca tcaagatggt ttagattaag acctcgaaca atacaatacc caatactctg
      181 tactgtattg tgaatttctt ctatcaaact attattgttt gcagataaaa tatacttttg
      241 attcatatag tgaattaaat ttaaaccagt acggcgttgg cttgccttgc cgtactattt
      301 gtactgtctg cggcttcgcc gccttgtcct gatttaaatt taatccacta taacatatcc
      361 ctgtcgtgaa atgctgtctg aaagggtttg cctgccctta cggcagcagc cttgccctga
      421 acatcgcctc cttcatttgg cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa
      481 tcagcgaatg cccgatgttc agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt
      541 tgtgtatggt cagtccgtgt ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg
      601 cgccgttttg cagacgctcg aattgcttga gttgttcggc gtggctgcgc gcgtcggcat
      661 acgcgcctgt gtgcagctcg acaacgggcg caccgacatc acgggcggct tggatttgcc
      721 tgtcgtcggc atcgataaac aaggacacgc gtatgcccgc atcggtcagg attttggtga
      781 actcggcaat tttgtcttgt tgcgccaata cgtccaaacc gccttcggtc gtgatttcct
      841 gccgtttttc aggcacgatg cacacgtctt ccggcatcac tttaagcgcg ttttcgagca
      901 tttcttccgt caacgccatt tcaaggttca ggcgcgtgcg gatggcgttt ttaacggcaa
      961 acacgtccgc atctttaata tggcggcggt cttcacgcaa gtgcatggta atcaaatctg
     1021 cgccgtgggt ttccgcaatc agcgccgctt cgacagggct gggataaatc gtaccgcgtg
     1081 cattgcggac ggtggcaata tggtcgatgt tgacacctaa aagcatgatg tttcctttcc
     1141 tcttcgtttt tctgtttggc ggcatggtgc gggcgcagct acgcggtatt gcggtcaaac
     1201 tgccttatct gttccaacac ctgccgcgat ttcagccctt cgggcaacag gtggcggata
     1261 aaaagccgtg taattttcaa tgcctgttgc aggctttcgg cggtgcggaa actgccttcg
     1321 cgcaaatcga tcaggctttg cccttcgaca attgcgccgg cttcgtgcga aagcgcgtct
     1381 gcgtcctcgc atacgggcat gaccgcctct tcgggcatca gccggtaggt tttgtccgcc
     1441 aaaatatcgc cgcccgtgcc gtcggcgtgc aaatcggggg caacgcccaa ggcattgagc
     1501 agcttccact cgaagcggcg caaatcggca atatggttgg cttcacggca caccgcctcc
     1561 atcacttttg ccaacgcgtc gtacagttcg ggcatcggat cttcgcgcgc ggtcagtttc
     1621 aacaccagtt cgttcacata cagaccgctg aacaaagccc taccctgcgg ctgcggccac
     1681 ccgcctatcc attcggcgcg atgtagggtc ttcaactctt gcgaaccata ccaagacgca
     1741 ctcaccggca caaacggcac caacacgccg cgcaattcgc tctgccgctt gcgcgcgctt
     1801 cttgccaaaa gtgccacacg cccgtaacgg cggctgaagg cttcgacccg caggctgctt
     1861 tcgcgccatg gcgaggaagc caacagaaag acgggttcgt ggttgactcg gtgttcggac
     1921 atcatgcctg aaatgccgtc tgaacgctgc tgccgctaca aaacggcggt cgggtacgaa
     1981 ccgatgactt tgacgaacga agcgcgttcg cccaagcgtt ccaatgccgt ctgaatctgc
     2041 gcgtcccggc ggtgtccctc gatatcgata aagaacaggt attcccacaa aacggatttg
     2101 ctcggacggc tctcaaactt ggtcatggaa atacccgact cggtcagcgg ttgcagcagc
     2161 gaggcaaccg caccggcgcg gttgggcgcg gaaacggcca gcgaagtctt gtcgctgccg
     2221 cttgcaccgg tttcgtgatg tcccatcacc aagaagcgcg tggtgttgtt cggttcgtct
     2281 tcgatgcact cggcaaccat atcgagtccg tagatttccg ccgccgtgcg tccggcgatg
     2341 gcggcaaccg taccgtcgtc cgattcggca accagccttg cggcttcggc attgctggac
     2401 acggcaatcc gttcggcgtt gggcaggtgt ctgcccaacc agtcgttgca ctgcgccaac
     2461 gcctgcgcgt gggaaaagac tttggcaatg ccttcggtgc tgccgttgtt tttacgcaaa
     2521 aggttgtggt ggatgcgcaa aacgacttcg ccgcacgcct gcaacgcggt aacggcaagc
     2581 aaatccaacg tgcgcccgac cgagccttcg gtcgagtttt ccacgggcgc gaccagataa
     2641 tccgcctgac gggtttcaac ctgcttgaag cagttgtcta tggtcggaca cgccatggtg
     2701 tgcgcggcgt gtccgaaatg cttgatcgcc gcctgctggg taaacgtgcc ctgcggcccc
     2761 agataggcga tggtcagcgg gcgttctacg gcgaggcact cgctcatcac ttcccgaaac
     2821 aggcgtgcta ccgattcgtc gggcagcggg cctttgttca aatcctgaat gcggcgcaac
     2881 acggcgactt cgcgttcggg acggtacact gcgcccgtgc ctttcagttc gccgatggcg
     2941 tgtgcgtgtt gcgcgcgttc gttgagcagg cgcaggattt ccgcatcaat ggtgtcgatg
     3001 gcgttgcggt ggggaaggag gagttcgtcg atagtttggg acataatttc gcctgaacag
     3061 taatttatcg tgcattctaa catcaaatgc cgtctgaaac ggtttcagac ggcattttcc
     3121 gttaaaggta ttcgctttgc ccgttttctt tccacgcacg atgcgagcag agccacagaa
     3181 gcgcaatgcc gcaagaggtg gacgcggtca tcgttgccgc catcacggtt gccgaaccgt
     3241 cgtgcaagaa ggtcgccgcc atacccaccc ccgcgccgat taaggactgg aacacgccca
     3301 acacggcgtt cgcgctgccg ccctcttctt tgaaataaga cataaagcat gcctgcgtgt
     3361 tcgcgccgac caagccctgc gtgccgacgg aaaacatcac gcacgcaacc aagagccaga
     3421 acggaggcaa cccgaaaaac agcacggcgg cgagttggga caggttggcg gcaaattgga
     3481 cgacaatccc ccacagcagg atgctttgcg gatgcgcgcc ggttttgagc cgccacgcgg
     3541 taatgcggtt gaaaaacatc atcgtgatga tgttgagtgc aaacgcccaa gcgtattgat
     3601 gaggcgtaac acggtaaagc tgctggtaca cgaaggaaga ttcggtcaga aaggcgaaca
     3661 tcgaaccgaa gctgaatgcc tgaaaaaaca gataacccat cgcggcacgg gttttcaata
     3721 cgcgtttgaa ccgcccagcc accagcccga acacatcccg tccgattttg ccgccgacgg
     3781 caggcttggg caggaaatac tgcaccaaac cgagcagcac cagcgaatac gccgccagaa
     3841 aaacaaaaat cgcctgccag ccacccaagc cctgcaacag tgcgccgacc atgggtgcga
     3901 ccagcggcac aaccatcaaa atgatgccga taagggcaaa catctgggcg gctttgcgtc
     3961 cggaataata atcgcgcacc attgcgccga cgatgaccac agtcatgccc gcaccgaatg
     4021 cctgcacgac gcgcaggttg aggagctgtt cggcactcga agcaaatacg atggcggcaa
     4081 cggcaaggca gtagacggcc agtccggtca gcgcgacggg tttgcgccct ttgatgtcgg
     4141 acaccgaacc gccgaccacc tgtccgaacg ccgtgccgaa cataaacaaa ctcaggctct
     4201 gttcgatgcg gtggacatcc gcgttcagcg actgcgccat ttcgggaatc gcgggcaggt
     4261 aggcatcgat ggaaaacggc atcagcgtaa ccagcatcgc catcaaaacc gccatcagtt
     4321 tttcgctcat ttcaggataa tgggcagaag gcataaattc tcgtccgatt cgcaaacaaa
     4381 ccgcccggcg gcggaaaaca caatacgcgg aaacggaaaa ttttaacaca ccggcacaaa
     4441 caggcggcaa caaaagcccc ggcaaaaccg gacgaaccgg aacgatatag tggattaaca
     4501 aaaaccagta cagcgttgcc t
//
LOCUS       DAXIEB010000130         4468 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000130 DAXIEB010000000
VERSION     DAXIEB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.130"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            408..483
                     /locus_tag="ACFOA3_001845"
     tRNA            408..483
                     /locus_tag="ACFOA3_001845"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:442..444,aa:Glu,seq:ttc)
     gene            1250..2056
                     /gene="thiD"
                     /locus_tag="ACFOA3_001846"
     CDS             1250..2056
                     /gene="thiD"
                     /locus_tag="ACFOA3_001846"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6898799.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2106..2975)
                     /gene="tehB"
                     /locus_tag="ACFOA3_001847"
     CDS             complement(2106..2975)
                     /gene="tehB"
                     /locus_tag="ACFOA3_001847"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6898800.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            3089..3526
                     /gene="rnhA"
                     /locus_tag="ACFOA3_001848"
     CDS             3089..3526
                     /gene="rnhA"
                     /locus_tag="ACFOA3_001848"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6898801.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1128 a         1278 c         1084 g          978 t
ORIGIN      
        1 atttaaaagt tctagattcc cgctttcgcg ggaatgacga agtttcagac ggcatcgccc
       61 gcctgttttg atatggcggc accccccccc cgacaaaaaa aacaatccgg aacgcatctg
      121 accgttccgg cttattttca ggcgaatccg ccgcatcaga acatactgcg cacgcccata
      181 ttgacctgcc aagtctggcg catcgtgtgc atcgaagacc tttgcgcctc aaaataaagc
      241 tgccttccgt tgtcggcatt accacgcaaa aaaatgaatt gcttgatatt ccaatgtttt
      301 tatatgtttt tatatggtga tgcgatcaga caaacgcccc cctgacattt gtttagacgg
      361 catcgtattg ctaaatttct ataagtatgt ataatgtccg tttccacgcg cccatcgtct
      421 agaggcctag gacactgccc tttcacggcg gcaaccgggg ttcgaatccc cgtgggcgtg
      481 ccaattcaaa aacctgcttg tttcaagcag gtttttttat tatgaatcgt cattcccgca
      541 cccttcgtca ttcccgcgaa agcgggaatc tggagcgtag ggttgaagaa accgttttat
      601 ccgataagtt tccgtgccga caggtctgga ttcccacttt cgtgggaatg acgagtcttt
      661 tataaccttt aaggctgctc tgcccaatgg tttggattcc cgcttatgcg ggaatgacgg
      721 ggcggggcga tgccgtctga aacttcgtca ttcccacaac ccttcgtcat tcccatgaaa
      781 acgggaatct agaacttctg aattttcaga cggctttata aatattgccg ctgccccaag
      841 ggctagattc ccgcctgcgc gggaatgatg atttggaaat cacccgaaac ccaaaaacaa
      901 ctgaaaccga acacactgga ttcccgcttt cgtaggaatg acggaatttt aggtttctgt
      961 ttttgtggga atgacgaata aagcgtgccg gtttatgctc accgcaacac gcggttcaga
     1021 cggcattgct ctcttttttc attatcagtg ggtgtagcaa ctgtattttt caccccgtcg
     1081 ggcaaaaata cagttgctac gatgcacccc gccgccctgc cctgtgcctt gtcctgcaat
     1141 acggcatata atgcaccaca aaccccgcgc tgcggttttc agacggcatc gccgtgcttt
     1201 tttacaggca ttagcccttt ttatcggaca caatattaag gaggaataaa tgaaaagctc
     1261 ttttgtgcaa acgcttacca tcgccggttc ggattcgggc ggcggtgcgg gcattcaggc
     1321 ggatttgaaa acgtttcaga tgcgcggcgt gttcggaacg tgcgtcatca ccgccgttac
     1381 cgcgcaaaat accttgggcg tgtcggcggt tcatctcgtc ccgaccgaaa ccatcaccgc
     1441 acaaatccaa gcaatcaggg aagactttga catccgcgcc tacaaaatag gaatgctcgg
     1501 cacggcggaa atcatcgaat gcgttgccga caagctgaaa cactgcgact tcggcaggcg
     1561 cgtactcgac cctgtgatga ttgccaaagg cggtgcgccg ctgttgcagg attccgccgt
     1621 tgcggcactg acgcgcctgc tgcttcccga tacggatgta ttgaccccca acctgcccga
     1681 ggcggaagcc ctgaccggcg tgcatatcga aaaccgtaaa gatgcggaac gtgcggcaaa
     1741 aatcctgctt gattacggtg tcaaaaatgt cgttatcaaa ggcggacatt tgaacggcag
     1801 cacaagcgga cgctgcacgg attggctgtt tacgcaaaat gaaacgctgg aattggacag
     1861 cccgcgcttt ccaaccgccc acacgcacgg cacgggctgc acgttttccg cctgcatcac
     1921 cgccgagttg gcaaaaggct cggacgtttg caaagccgta cggactgcca agtcctacat
     1981 tacggcggca atctcaaacc ctttggaaat cggcgcggga cacggcccgg tcaatcattg
     2041 ggcgtatcgg gactagacgt aaaaatgccg tctgaaacaa aatgttcaga cggcattttt
     2101 gaggattatt caggcttttt cgccagcatg gttacaaatt taaaccgtat cggattgccg
     2161 ttttcgtctt tggcgtgcat cgcgcccaat tcttctttat attcgaccag ctcccaatcc
     2221 cgataataat ccttcagctc tccctcttta aatttaaaag ggaacggcat cggacaaggg
     2281 aaatccgccg tatccattgc cgacacaatc aaattgtacc cgcccgccgc cgtatgcgcc
     2341 tgcatatcgg caatcacgtc gggcacgcgc tgcggcatca ggaacatcag caccactgtt
     2401 gccacaatat aatcaaactc accctgcaag gcggcggcat tcaagtcata ttccagcgtg
     2461 cggacgttca agccctccgc ctctgccagc tccgccacgt ttgccaaggc ggccggattg
     2521 tgatcgactg cagtaacttc aaatcccttc aaaccgagaa acagtgcgtt gcgcccctgt
     2581 ccgcagccca tatccaacgc cctgcccgcc ggtacggtat cccgtgccgc cgcgaccgca
     2641 gaatgcgtgg cactcatccc gtattttttg tgaaaatagt ctgccgccgc gcaatacagc
     2701 gacaaacgga tttcggcatc gtccgttttc agtttgaccg aaaacacctg ctgcggcgca
     2761 aacacgcaat cgccgctttc cgccgaccaa acctctgccg ccccgtcctg cgtgcggact
     2821 tcgacatcgc cctgcaacac attcaggcag acccattccc ctccttcgga cgaataaccc
     2881 gacaacaaaa tttccggcag gttttccgcc ttccatacgg gcatttgtcc gaaacaaaac
     2941 aactcgccgc tttgacccac tatccgctcc ttcatattca aaaataaagt tgcacattat
     3001 atgcctattt taatccgccg caatctttca gacggcacgg cgcacaaacc gcttataatc
     3061 acgccggaca ccacacaaag gcacaataat ggaccaaacc gtttaccttt acaccgacgg
     3121 cgcgtgcaaa ggcaatcccg gcgcgggcgg ctggggcgtg ttgatgcgct acggcagcca
     3181 cgaaaaagaa cttttcggcg gcgaagcgca aaccaccaac aaccgcatgg agctgaccgc
     3241 cgtcatcgaa ggactgaaat cgctcaaacg ccgctgcacc gtcatcatct gcaccgactc
     3301 gcaatacgtc aaaaacggca tggaaaactg gatacacggt tggaagcgca acggctggaa
     3361 aaccgccgcc aaacagcccg tcaaaaacga cgacttgtgg aaagaactcg acgttctggt
     3421 tgggcagcac caagtcagct ggacttgggt taaaggacac gcgggacacg cggaaaacga
     3481 acgcgccgac gatttggcaa accgtggcgc agcgcagttt tcctgactgc cgctccggca
     3541 aaaatgccgt ctgaaaccgc taatgggctt tagacggcat cgtcctccac cgtcattccc
     3601 gcgcaggcgg gaatccaaac cgtcgggcaa cggcaatatt caaaagccgt ctgaaaattt
     3661 agaggctcta gattcccgct tttgcgggaa tgacgaaaag ttgcggacag cagaggaatg
     3721 acgaggtttc agacggcatt gggggctttg tcgggcttca ggcggcatcc gaccgccccg
     3781 tcattcccgc gaaagcggga atctaaaaac ccaacgctgc aaaaatttat ttagaaacaa
     3841 ctgaaaccgc tccaccgtca ttcccgcgca ggcgggaatc tagaccgtcg ggcaacggca
     3901 atattcaaag attatctgaa agtttgaagt tctagattcc cgttttcacg ggaatgacga
     3961 aaagttgcaa gaatgacgga gtttcaggcg gcatccgacc gccccgtcat tcccgcgaaa
     4021 gcgggaatct aaaaacccaa cgctgcaaga tttatcagaa acaactgaaa ccgaacggac
     4081 tggattcccg cctgcgcggg aatgacggga ttttagtaac cgtagcaacc gcctgcgcga
     4141 cggctaaggg gcttcagcaa ccgtagcaac tgcctgtgtg ggaatgacgg acaatgggct
     4201 tcagacggca tcagacggca tctcttgcct gccgctaaaa cagtttgccg cacaactgtt
     4261 caaacgcgtc cgatatattt caacacacag gacgacacat aaagcacctc cctatgtgtc
     4321 gtcctgattt ggaaggggtt acaccccctc ccaaataaag tctgatcctg ccgccccgaa
     4381 ggacagatgt ccgagtggcg gggtttcaac cgaaaaggaa atacgatgaa gtggtacaat
     4441 tagcggcaat gcggacagac aaattaaa
//
LOCUS       DAXIEB010000131         4446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000131 DAXIEB010000000
VERSION     DAXIEB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.131"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(63..1205)
                     /locus_tag="ACFOA3_001849"
     CDS             complement(63..1205)
                     /locus_tag="ACFOA3_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6898802.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="ACFOA3_001850"
     CDS             complement(1254..1676)
                     /locus_tag="ACFOA3_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898803.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="ACFOA3_001851"
     CDS             complement(1669..2478)
                     /locus_tag="ACFOA3_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6898804.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFOA3_001852"
     CDS             complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFOA3_001852"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6898805.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="ACFOA3_001853"
     CDS             complement(3137..3904)
                     /locus_tag="ACFOA3_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6898806.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4446)
                     /locus_tag="ACFOA3_001854"
     CDS             complement(3914..>4446)
                     /locus_tag="ACFOA3_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6898807.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1096 a         1118 c         1080 g         1152 t
ORIGIN      
        1 atcggccgga tattaaaaaa gccgtctgaa catttttcag acggcttttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcagtc gaggttgccg
      121 ttataaagcg gaattttccg cttgggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca taccgacctg ccagccggca tagtgttttt tcaaccacgt ccccaactgc
      241 ggatacagtg cctgcaaagc gcggacttcc tcaaggcgca caccgtaggg cggattggac
      301 accataatgc cgcccacgcc gttcggccgg acggactgcg catcggcaac gctgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcccgaaat cggtgcaaaa gcaggtttga cctgcgcttc ggcacggcgg
      481 cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt tctcaaaacc gaagcggcgc
      541 atcatacccg gcgcgcggcg ggcggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc aataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgtaaag taaaccaatt aaaccagttg
      961 atatttttgg cgagtttgta gatgtcgcgc tcattgcggt atgtcccttt ggtcaggcgc
     1021 agcaggatac ggctggcagt acgcgaatgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacact tgtacatcgg tacagccgag gctttcgagt
     1141 tcttgagata aaacggtctc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccgactg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccggcggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccacg accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcaac caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcagggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 gaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatcat
//
LOCUS       DAXIEB010000132         4382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000132 DAXIEB010000000
VERSION     DAXIEB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.132"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(191..2119)
                     /gene="dnaK"
                     /locus_tag="ACFOA3_001855"
     CDS             complement(191..2119)
                     /gene="dnaK"
                     /locus_tag="ACFOA3_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6898808.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2327..2599)
                     /locus_tag="ACFOA3_001856"
     CDS             complement(2327..2599)
                     /locus_tag="ACFOA3_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898809.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2785..3471
                     /locus_tag="ACFOA3_001857"
     CDS             2785..3471
                     /locus_tag="ACFOA3_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6898810.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3603..3941
                     /gene="erpA"
                     /locus_tag="ACFOA3_001858"
     CDS             3603..3941
                     /gene="erpA"
                     /locus_tag="ACFOA3_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6898811.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3958..4104
                     /locus_tag="ACFOA3_001859"
     CDS             3958..4104
                     /locus_tag="ACFOA3_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898812.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4101..>4382
                     /locus_tag="ACFOA3_001860"
     CDS             4101..>4382
                     /locus_tag="ACFOA3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6898813.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYSGLTKTSTAL"
BASE COUNT         1017 a          879 c         1147 g         1339 t
ORIGIN      
        1 aggcaacgct gtactggttt aaatttaatc cactatataa aaaatttcca gagaaccgat
       61 acaacagttg gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat
      121 gctatggtct tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag
      181 acggcatcaa ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat
      241 cgtctttctt tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg
      301 cttgagcgta aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg
      361 catcgatggc ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt
      421 cgattttttc tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca
      481 cggagtgaat cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat
      541 cttcctcagc attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac
      601 ctgaagaacc ttggatggta atgttggccg ctttgccagt gcctttgtct ttggcggaaa
      661 cgtgcaagat accattggcg tcgatgtcga aagtaacttc aatttgcggc ataccgcgtg
      721 gtgcaggtgc gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt
      781 cgcgttcgcc ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga
      841 acacttgcga cgctttagtc ggaatagttg tgttcttttg aatcagcttg gtcatcacgc
      901 cgcccatggt ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc
      961 ggccgccgct taacacttcg ccttgaatcg ctgcgcctac ggcaacggct tcgtcggggt
     1021 tcacgtcttt gcgaggctct ttaccgaaga aggctttaac ggcttcttgt actttcggca
     1081 tacggctttg accaccgacc aagattacgt cgtcgatgtc gccggtgctc agaccggcat
     1141 ctttcaatgc ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt
     1201 cgaatttggc gcgggtaatt ttcatcgcca agtgtttcgg accggttgcg tccatggtaa
     1261 tgtacggcag gttgatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg
     1321 cagcttcttt cagacgttgc agagccatta cgtcttgttt caaatcaatg ccttgttctt
     1381 ttttaaactc gtcaatgatg tagtcgatca agcgttggtc gaagtcttca ccgcccaaga
     1441 aagtatcgcc gttggtagcc aatacttcaa attgtttgtc gccgtcgagg ttggcgattt
     1501 cgatgatgga aatatcgaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt
     1561 tgttgtcgcc tttgtccata ccgaatgcca aagcggctgc ggtcggctcg ttgatgatgc
     1621 gtttcacgtc caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt
     1681 tgaagtaggc agggacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg
     1741 cggcttcttt cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc
     1801 cttgtgcttt tacccatgcg tcgccgttgt tggctttgat gatttcgaaa ggcatagatt
     1861 cgatgtcgcg ttggacttct ttgtcttcaa atttgtggcc gatcaggcgt ttggcggcgt
     1921 aaatggtgtt tttagcgttg gttacggctt ggcgtttggc aggcgcaccg acgaggattt
     1981 cgccgccgtc caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga
     2041 tcactttggt ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga
     2101 taccgattac ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg
     2161 ttggaacatt ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac
     2221 agcatggcta taagtatata actttatgaa tatattggtt ttatatttga tttaatacat
     2281 ttggctccaa tgcatttaag cataatattt caaatggcag gcaggtttat tcatagacga
     2341 tgccggcgag catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag
     2401 actcgatgat gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt
     2461 tcagtatgtt gaatttttcg cacagggatg cggataaagt gatgtcaggc tgtttggctt
     2521 cagaacggtt ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat
     2581 atttgccggt tttcttcatt ttctatcctt tttctgtcaa ctcaggatta aacctatgga
     2641 aaatcttaaa aattatgtat tgagtaagaa aaatcataat ttaaatttag tttatcatag
     2701 ttgttccgtt tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga
     2761 atgaagggga gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg
     2821 aaaagcgaag cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc
     2881 agcaggatat ggaatgaagg cggtctgccg aagtctgaaa cattaaaaaa aatcaagcag
     2941 ttgaaggggt gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa
     3001 gccccaaaaa aatcccttgc ttacgatacc ttgggcaatg aagttgatac ggacgagttt
     3061 gtctttgtgc cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat
     3121 gaggaaccgg tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc
     3181 gatacgaaaa acctgtctgt aatttccgtc aagggggatt ctatggaggg ggttttgaat
     3241 gacggcgatt cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg
     3301 ttgcggatta atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc
     3361 aatgtgattt ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc
     3421 gatgatgtgg agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc
     3481 ttgaaattgc aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc
     3541 tgcttgcctt taaagggggt ttgagagaga atgcagaaaa tattatatta aggaatcaca
     3601 ccatgtcgga tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt
     3661 tgattgccga agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct
     3721 gttcgggttt ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa
     3781 ttgagaaaaa cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg
     3841 cggaaatcga ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg
     3901 cggaaacaac ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg
     3961 ccgtctgaac gttcagacgg catttttact tttggaaaat atattatcgg gatgaattca
     4021 catataatcc gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta
     4081 tcaggcgtat ttgttccctt atgattgaga tgagtaaaga ttaccgaaat gatttgtacg
     4141 atgtatatgt ttcctacccg ccccaagtgg atcgcgggct tatccgggag tgcctcaggg
     4201 agaatctcgg cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag
     4261 tgctggttga ggaaaaatgc acttgggcga aacgggaaga gttgcacgat tatttcagct
     4321 atttgggttt ggatattatt acccggagat atagtggatt aacaaaaacc agtacggcgt
     4381 tg
//
LOCUS       DAXIEB010000133         4356 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000133 DAXIEB010000000
VERSION     DAXIEB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.133"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            1..605
                     /locus_tag="ACFOA3_001861"
                     /pseudo
     CDS             1..605
                     /locus_tag="ACFOA3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(741..2144)
                     /locus_tag="ACFOA3_001862"
     CDS             complement(741..2144)
                     /locus_tag="ACFOA3_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6898814.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2213..3640)
                     /locus_tag="ACFOA3_001863"
     CDS             complement(2213..3640)
                     /locus_tag="ACFOA3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6898815.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLYDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3982..4218
                     /locus_tag="ACFOA3_001864"
     CDS             3982..4218
                     /locus_tag="ACFOA3_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898816.1"
                     /translation="MPTKNLGKILSGSLTAAAILMLFVWIIPKQFGPSKEEIQAQEEW
                     DRKYKEAEAVFNEQCKTAGERFTRRRTMWKGLCC"
BASE COUNT         1037 a         1148 c         1066 g         1105 t
ORIGIN      
        1 tttgctatgc actcaaacag atggggataa cgcgcaaaaa aagaccacca cttacaaaga
       61 acaagacccg gccaaagtaa cgcattattt gacacagccg gccgaatttt ccgactacca
      121 acgtgtttat ttggatgaaa caggatttga ccgctacctg ttccgtccct atacccacag
      181 cctgaaaggg caaatagtaa aagcgcagat aagtggaaaa agataccaac gcttatcttt
      241 ggtgtccgca caagtcggca accggctgat tgctccgatg gtttatcaaa atacgatgat
      301 cggagtcttt tttgaagcgt ggtttcagca atgcctactg cccgcattga ctcaaaaatc
      361 ggtgattatt ttagataatg cgcgatttca ccgtatgggt gtcttacggg aaatggcgga
      421 aaaattggga cataaggtat tgcctcttgc accttattta cctgagctca acccgattga
      481 gaaagtgtgg gcgaatatta agcggtatct gagaaccgtt ttgtctgatt acgcccgatt
      541 tgacgatgca ctactgtcct attttgattt taattgaata tacatttgaa ttaattcgca
      601 cttaatttaa atgtgttttt aactgtgctt tatttaaagg caatgagaat gtgaaagtat
      661 cggatcaatc ccaaagcagc ctgcactttc gaaacggggt gcaggctgct ttgggaattt
      721 cataaccgtt tcagcctgct ttattccgca aataccgttt ccaaccctaa cccgctctct
      781 ttcaccaagc gcaaataagc cagcatgaat ttataccgtg cttgagccag tttctgttct
      841 gcttgggcga cttcctgccg cgcccgtatt acttccagcc ggttgcggat gccgtattgt
      901 tggccggttt cggtcgattt cagtttcaaa cggctgcttt ccaaaacccg ttcttgcgcc
      961 atgatttggt aacgcgccgc accgctttcg gtataagcct ggcgtacggc gagtttgatg
     1021 tgccgctcgg ttgcggtcag ctgtgcttcg gcggccccgt attgcgcttc ggcttcatgg
     1081 attttgcccg acaattctcc gccggtataa agcggcaaat tcaactgtac gccgacgctc
     1141 atccctttgc cccgatagtg gtagtcatta ttctgcgcag atgaagtgta gaggttattc
     1201 tgatagccga catgggcaga aacggtggga tagcggctgt tctgtgctgc ccgaagcgcc
     1261 tgtccgctgc tttgcagggc aagctgctgc atccggtatt catgattgtt ggataaggca
     1321 atgcgctgcc attcatccag actgtaacgt tccagcttgg gcagatagcg tgccaacagg
     1381 ttggcggtat ctatggcctc gatttgtttg ctgtccaggc cggtgtagtc gttcaactgg
     1441 ttttcatagg tttgtttctc agccaatacg gcgatttctt gggccagggc attgtcgtaa
     1501 ccggctttgg cttcgtgaat atccagcgcg gtggcagcac ctttattgaa taaagcctgc
     1561 gcctgcctta cctgctgggc ataagcctct ttttccgccg catgggcggc aacggtgtct
     1621 cggctgagta aaacgttgaa ataactttcg gcaactttca acagcaattc ttcgcgtgcc
     1681 gcatcgaaac gctgttctgc agcctgcgta tcgaacctgc tttggcggta ttgtgcaaat
     1741 ttggcagcgt caaataaggt ttgtcccacc tgcacgctcc atccctgtgt ttcgcgggtg
     1801 gaagaaatcg atggcggctg gcgctggtag ctggcattgg cggatacatg gggaaggaat
     1861 gcggccttgg cttgttgttg ccgtgcgcgc actgcatcac gctggtaatg ggacgcttga
     1921 aaatcagccg aatgttgctg cgccgcccgc catgcttcag gcagcgtaaa agccgaaacg
     1981 gatggggaaa gggatagtgg caaggtaaaa agtgaaacgg gtaggatata tttggaaaaa
     2041 taggatttca tagccgaaaa tagttcatgt tgcaaatagg gcgtcagtgt caggcaaacg
     2101 gaaataccgt aatcttgcat tatcattaga ttgagcaatg tcatccgggc aatggtttca
     2161 ggcagtctgc atgtccgaac cggcggataa caaatgccca gtatggatcc gcctatcgct
     2221 ccctaaagct ttcgtccaat ttggtttgca gcgggcttaa cagataatcc agcacccgcc
     2281 gtttacccgt tttaatctcc gccgtgacat tcatgcccgc cgtcagattc actgctttgc
     2341 cgtcaatatt caaggtatgt ttgtccagcg acaccaccgc cgtataaacc aagcccaact
     2401 gttcgtggct taccgcatca tggctgacac ttttcacctt gcccgtcaga taaccgtagc
     2461 gcgtataggg aaaactctca atcttcacca ccgcatcctg tccctgttcc acaaaaccga
     2521 tgtctttgtt caataccaaa acttccacgt ccattttgtc gtcatcgggc gcaaccacca
     2581 tcattttttg ggcagcctgc accacaccgc ccaccgtata ggtggccaat tcctgcaccg
     2641 tgccgtccgc aggcgactgt attgtcatca gctgctgccg ctgctttgcc ttatccgttt
     2701 ggccgcggta ttggtcaatc tgttcgtttg cctggcgcag cgcatccagc gtatcgcgtt
     2761 tcaggttctg cgtattcagc acccgactct gctccgcctg tgcaatggcc gcctgaatct
     2821 gcctcatctg accgcgcgta ctttccaaat cgttccaatt gctgaccgat ttgctctgct
     2881 gctccaaaaa cgcatgttcc gaaataaaat tgtcggcccg caaacggcgg tagtctgctg
     2941 ttttctgctg ctcgatcgcc cccaccgaaa ccagcttctg ctcctgcgcc ttggccgact
     3001 gcaattccgc ctgatggccg cgcaaagccg actgcaattg cgcatcctgc gccgcccatg
     3061 cctgatactg gtgctgcgcc aacacctgcg ccgattgcac atcggcatcg tagagaccta
     3121 aagaccgtgc ttgcgccata tcgatatgcg gcacggtacg gctttccaat gccgccaata
     3181 ctgcttcata acgcagtttg gacaattggg cagcctgcaa agcctgctcc gactgcacca
     3241 catcgctgtc tgttcccaca gcctccagtt ccgccagcgt ttctccctgt ttcacatgct
     3301 gcccgtcgcg cacatgtacc gccttaacca ccgccgtttc cagcggctgg atggttttgc
     3361 tgcgcccgcc cgacaccgtt ttgcccgaag ccgccgccac aatatcgatt ttgccgaacc
     3421 aggaccacaa caaagccaaa agcgcaaacg ccataataaa acgcgccgcc catttcggag
     3481 cggcagagac cggcgtatcg gtcagttcca aatgcgcggg caaaaacgcc tgttcttccg
     3541 ccgtgcgttt gggcggttcc aactggtcgc gcaccgccca aacattgcgc catacagtaa
     3601 tgtagcgaga aagaaaggat ttcagggcgg agaaaaacat aacgggtata accttggcaa
     3661 tatagaaaca ggaaacaata taaatatgta aaggaatttt aacggaaagc gcggcagctg
     3721 ttaagggaaa ggcgggaata ttgacaaaaa atacccaagt cgttacaaat attcattatt
     3781 ttactgcgta acgcaacgct gaagcgcagg ctgcttttga gatgcggcaa ggttcggcaa
     3841 aaagcagcct gcacatttaa ccacaggaac tacccatgtt taccacaaac gatttacgcc
     3901 atttcctaga aggtttggtc atcctattcg caatcggcta ttgggacacc atgctgctgt
     3961 tgctttggtt tctcgtccgc tttgcctaca aaaaacctcg gcaaaatcct ttcaggcagc
     4021 ctgaccgccg ccgctatcct gatgctgttt gtttggatta ttcccaaaca attcggcccg
     4081 agcaaagaag aaatacaggc acaagaagag tgggacagaa aatacaaaga agccgaagcc
     4141 gtgtttaacg aacaatgcaa aacggcgggg gaaagattta ccagacggcg gacaatgtgg
     4201 aagggattat gctgttgaag gtagtacctg agcgtaccgt ttcggcagat gcaaaaacca
     4261 gagacccgat gtgggacaat gcggctttac agaccagcga aggcgtaaat tttattgctc
     4321 gtttcctagg attttttagc gatggggaat accgct
//
LOCUS       DAXIEB010000134         4256 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000134 DAXIEB010000000
VERSION     DAXIEB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.134"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..593)
                     /locus_tag="ACFOA3_001865"
     CDS             complement(<1..593)
                     /locus_tag="ACFOA3_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6898817.1"
                     /translation="MDRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPLADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFAAHPDAETPPPQN
                     SVDEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQG"
     gene            complement(604..1398)
                     /locus_tag="ACFOA3_001866"
     CDS             complement(604..1398)
                     /locus_tag="ACFOA3_001866"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6898818.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQTAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1782..3116
                     /gene="gdhA"
                     /locus_tag="ACFOA3_001867"
     CDS             1782..3116
                     /gene="gdhA"
                     /locus_tag="ACFOA3_001867"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6898819.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            complement(3405..4184)
                     /locus_tag="ACFOA3_001868"
     CDS             complement(3405..4184)
                     /locus_tag="ACFOA3_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6898820.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWAV"
BASE COUNT         1102 a         1232 c          985 g          937 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcatccac gctgttttga
      121 ggcggcggcg tttccgcatc gggatgagcg gcaaaataat cggcatacag tttccacaaa
      181 acctgaaccg tcggatcgaa tgccccttcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cagcggcgaa gtcagataac cgtcacggtt cagacggctg
      301 atcaggctgt cttcacggta aaggctgaac attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcacgcctg tcgtccacac cgaacagacg gagctccgca
      421 ccggaaccca gtgcgacacc tttccagaaa aacacatttt cattgcgttt ttcatccccg
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaaccgt cagaaaacgc ctgcgcccga aatgcctgtc catatacccc
      601 tcctcagacc gacactgccg ccttgatgtg cggatggggg tcgtagcctt ccaactcgaa
      661 atcttcaaac ttgaaggaaa acaaatcttt gacttcagga ttgattttca tcactggcaa
      721 ggcgcgcggt tcgcgttcca actgcaatgc ggtctgctcg aaatggttgc ggtacaaatg
      781 cgcgtcgcca aacgtatgga caaactcgcc cgcctccagc ccgcacactt gcgccatcat
      841 catggtcaac aatgcgtagc tggcaatatt aaacggcaca ccgaggaaaa tatccgcgct
      901 gcgctggtaa agctggcagg acagtttgcc gtcggcaacg taaaactgaa acagcgcgtg
      961 gcagggcggc aaggccattt catcgaccaa agccggattc cacgccgata caatcaggcg
     1021 gcgcgagtcg gggtttttct taatttgttc caccacattg gcgatttggt cgatatgcct
     1081 gccgtcgggc gcgggccagt tacgccactg gtagccgtaa accgggccta agtcgccgtt
     1141 ttcgtccgcc cactcgtccc aaatggaaac attgttgtcc tttaggtatt tgatattggt
     1201 atcgcctttg agaaaccaaa gcagctcgtg gataatcgag cgcaaatgca gctttttggt
     1261 cgtcagcagc ggaaaacctt tgcccaagtc aaaacgcatc tgataaccga atacggagcg
     1321 cgtgcccgta ccggtgcggt ctgatttgtc cgtaccgttg tcgaggacgt ggcgcatcaa
     1381 gtccaaatag gctttcatag cagtctttca tcaaattaaa cggcgcatat tgtaacattt
     1441 ccggataatg cccaaaacac ggatacaggc aggcaggatt gttggcaatt tcagtccttt
     1501 tccacagtaa aacccagtgg gaaaacaaaa ttaccttgat tggaatcaaa aaatctagtt
     1561 taattactta gaataaaatt tcaataatat ttttatttac gaaattaatt tatgattatt
     1621 gattaattat cggcaacaaa catcaaacat cgaaaacatg gaaaaaataa tgtcaacaat
     1681 ttttgccaaa tcgggcttgg catcagaaaa aaataggttt atattcccac ctacaaattt
     1741 gttttcccat tagtacacta tcaaccaaaa ggagtatccg aatgactgac ctgaacaccc
     1801 tttttgccaa cctcaaacaa cgcaatccca atcaggagcc gttccatcag gcggttgaag
     1861 aagtcttcat gagtctcgat ccgtttttgg caaaaaatcc gaaatacacc cagcaaagcc
     1921 tgctggaacg catcgtcgaa cccgaacgcg tcgtgatgtt ccgcgtaacc tggcaggacg
     1981 ataaagggca agtccaagtc aaccggggct accgcgtgca aatgagttcc gccatcggtc
     2041 cttacaaagg cggcttgcgc ttccatccga ccgtcgattt gggcgtattg aaattcctcg
     2101 cttttgaaca agtgttcaaa aacgccttga ccaccctgcc tatgggcggc ggcaaaggcg
     2161 gttccgactt cgaccccaaa ggcaaatccg atgccgaagt aatgcgcttc tgccaagcct
     2221 ttatgaccga actctaccgc cacatcggcg cggacaccga tgttccggcc ggcgacatcg
     2281 gtgtaggcgg gcgcgaaatc ggctacctgt tcggacaata caaaaaaatc cgcaacgagt
     2341 tttcttccgt cctgaccggc aaaggtttgg aatggggcgg cagcctcatc cgtcccgaag
     2401 cgaccggcta cggctgcgtc tatttcgccc aagcgatgct gcaaacccgc aacgatagtt
     2461 ttgaaggcaa acgcgtcctg atttccggtt ccggcaacgt ggcgcaatac gccgccgaaa
     2521 aagccatcca actgggcgcg aaagtgctga ccgtttccga ctccaacggc ttcgtcctct
     2581 tccccgacag cggtatgacc gaagcgcaac tcgccgcctt gatcgaattg aaagaagtcc
     2641 gccgcgaacg cgttgccacc tacgccaaag agcaaggtct gcaatacttt gaaaaccaaa
     2701 aaccgtgggg cgtcgccgcc gaaatcgccc tgccctgcgc gacccagaac gaattggacg
     2761 aagaagccgc caaaaccctg ttggcaaacg gctgctacgt cgttgccgaa ggtgcgaata
     2821 tgccgtcgac tttgggcgcg gtcgagcaat ttatcaaagc cggcatcctc tacgccccgg
     2881 gaaaagcctc caatgccggc ggcgtagcaa cttcaggttt ggaaatgagc caaaacgcca
     2941 tccgcctgtc ttggactcgt gaagaagtcg accaacgcct gttcggcatc atgcaaagca
     3001 tccacgaatc ctgtctgaaa tacggcaaag tcggcgacac agtaaactac gtcaatggtg
     3061 cgaacattgc cggcttcgtc aaagttgccg atgcgatgct ggcgcaaggc ttctaagcaa
     3121 acgccgccgc tccgcaaaca aaatgccgtc cgaaccgcaa atgctgttca gacggcattt
     3181 cattatccgc ccgttcgaat cgggtgatac ttatagtgaa ttaacaaaaa tcaggacaag
     3241 gcggcgagcc gcagacagta caaatagtac ggaaccgatt cacttggtgc ttcagcacct
     3301 tagagaatcg ttttctttga gctaaggcga gccaacgccg tactggttta aatttagttc
     3361 actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac cgcccaatcc
     3421 tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg gctctgatat
     3481 tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc cgcctccgaa
     3541 gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg cgccatcagt
     3601 ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt ctgccggtac
     3661 agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc cgcatccgca
     3721 atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc gctttcgcac
     3781 gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc cgtccggcgt
     3841 tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa aacatcctgt
     3901 tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc ctgctgcggc
     3961 ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg ggcgaccaaa
     4021 acattcaatg ccgacctgac cgacgggcgc gaaacgccga actcttccgc caaaacgcgt
     4081 tcgggcggaa tcttgccccc ttccgcgtaa accccttcca cgatgcggcc ttccaatacc
     4141 gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc tcctttataa
     4201 agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg acaagg
//
LOCUS       DAXIEB010000135         4177 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000135 DAXIEB010000000
VERSION     DAXIEB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.135"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            50..2107
                     /gene="metG"
                     /locus_tag="ACFOA3_001869"
     CDS             50..2107
                     /gene="metG"
                     /locus_tag="ACFOA3_001869"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6898821.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHSINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(2182..3135)
                     /locus_tag="ACFOA3_001870"
     CDS             complement(2182..3135)
                     /locus_tag="ACFOA3_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6898822.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVHD
                     AHVVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            3258..3485
                     /locus_tag="ACFOA3_001871"
     CDS             3258..3485
                     /locus_tag="ACFOA3_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6898823.1"
                     /translation="MLKTLTFAALHFTTALSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTKTSTALPRLAVLFVLSAASSPCPDFC"
     gene            3656..3985
                     /locus_tag="ACFOA3_001872"
     CDS             3656..3985
                     /locus_tag="ACFOA3_001872"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6898824.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            4014..4177
                     /locus_tag="ACFOA3_001873"
                     /pseudo
     CDS             4014..4177
                     /locus_tag="ACFOA3_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
BASE COUNT         1108 a         1255 c          963 g          851 t
ORIGIN      
        1 tttgatttgc ggtataatcc gcctttaccc tttgtttgca aagcacaata tgacacgcaa
       61 aatcctcgtt acctccgccc tgccctatgc caacggcagc atccacctcg gccacatggt
      121 cgaacacatc caaaccgacg tttgggtgcg ctttcaaaaa ctgcgcggcc acgagtgcca
      181 ctactgctgc gccgacgaca cccacggcac gcccgtgatg cttgccgcgc aaaaacaagg
      241 cattgcgccc gaagacatga ttgccaaagt gcgcgaagaa cacctcgccg actttaccgg
      301 ttttttcatc ggctacgaca attattacag cacccattcc cctgaaaaca aacagttttc
      361 ccaagacatt taccgcgcac tgaaagccaa cggcaaaatc gaaagccgcg tcatcgagca
      421 gcttttcgac cccgaaaaac aaatgttcct gcccgaccgc ttcgtcaaag gcgaatgccc
      481 gaaatgccac gcccaagacc aatacggcga caactgcgaa gtctgcggca cgacctattc
      541 cccgaccgaa ctgatcaacc cgtattccgc cgtatccgga gccaaacccg aattgcgcga
      601 atccgaacac ttcttcttca aactgggaga atgcgccgac ttcctcaaag catggacttc
      661 cggcaacaac ccgcacgacg gcaagcccca tctgcaagcc gaagccctca acaaaatgaa
      721 agaatggctg ggcgaaggtg aagaaaccac cctgtccgac tgggatattt cccgcgacgc
      781 gccgtatttc ggttttgaaa tccccgacgc gccgggcaaa tacttctacg tctggctgga
      841 cgcgcccgtc ggctacatgg cgtcgtttaa aaacctgtgc gaccgcatcg gcgtcgattt
      901 tgacgaatac ttcaaagccg acagccaaac cgagatgtac cacttcatcg gcaaagacat
      961 cctctatttc cacgccctgt tctggcctgc catgctgcat ttctccggcc accgcgcccc
     1021 gaccggcgtg tacgcacacg gctttttgac cgtagacgga caaaaaatgt ccaaatcgcg
     1081 cggcacgttt atcaccgcca aatcctatct ggaacaaggt ttaaaccccg agtggatgcg
     1141 ctactacatc gccgccaaac tcaacagcaa aatcgaagac atcgatttga acctgcaaga
     1201 ctttatcagc cgcgtcaaca gcgacctcgt cggcaaatac gtcaacatcg ccgcccgcgc
     1261 ctcaggtttc atcgccaaac gctttgaagg ctgtctgaaa gacgttgccg acagcgcatt
     1321 gctggcaaaa ctgaccgcac aaagcgaagc cattgccgaa tgctacgaaa gccgcgaata
     1381 cgccaaagcc ctgcgcgaca tcatggcctt ggcagacatt gtcaacgaat acgttgatgc
     1441 caacaaacct tgggaactcg ccaaacaaga aggccaagac gaacgcctgc acgaagtatg
     1501 cagcgaactc atcaacgcct ttaccatgct gaccgcctac ctcgcccccg tgttgccgaa
     1561 agtggccgaa aatgccgcca aattcctgaa tttggaagcc atcacttggg caaatacacg
     1621 cgaaaccttg ggcaaacact ccatcaacaa atacgaacat ttaatgcaac gagtggagca
     1681 aaaacaagtg gacgatttaa tcgaagccaa caaacaaagc attcagacga cccccgcgcc
     1741 tgccgccgaa gagagcaaat acgaaaaagt cgccgaacag gcgagcttcg acgactttat
     1801 gaaaatcgat atgcgcgtcg ccaaagtatt gaactgcgaa gccgtcgaag gcagcaccaa
     1861 actcttaaaa ttcgacctcg atttcggttt tgaaaaacgc atcatcttct ccggcatcgc
     1921 cgcgtcttac ccaaatcctg ccgaattgaa cggccgcatg gtcatcgccg tcgccaactt
     1981 cgccccgcgc aaaatggcaa aattcggcgt atccgaaggc atgattctct ccgctgccac
     2041 ggcagacggg aaactgaagc tcttggatgt ggatgcaggc gcgcaaccgg gcgacaaagt
     2101 cggttaagta gaaatctcaa aaaatgccgt ctgaaccctt tgggcgttca gacggcattg
     2161 tttttcgtta tcccgacaag ctcaaactac ccggttttgc gcctcgcctt tcacaaaggc
     2221 gtgaatgttc gccaacaata tatcgaacag cctgtccaaa gcctcgcggc tcgcccacgc
     2281 ggtatgcggc gtaacaatca gattgggaag tcgtgcattc agcaggggat tgccgcccct
     2341 tggcggctcc tccgtcaaaa catcgacacc agccccgccg atctgcccgt atttgagtgc
     2401 ggcaagcagc gcgttttcat ccaccagccc gccgcgccca caattgatta aaaccgcgcc
     2461 aggcttcatc tgccgcaatt cgttttcgcc tatcatattt tcagtttggg cgtttagcgg
     2521 acagtgcagc gacaacacat cagcagcccg taccgcatct tcaaaggaaa catagccttc
     2581 gcgcacagcg gacgcgtgtt tgtgttcggc aaacaccacc cccataccga atgcctgcgc
     2641 gtatccggca agcgtccgtc cgatattgcc gcgtccgaaa accgccagcg ttttgccgtt
     2701 caaatcccga atcggcgcgc cgtaatggca gaaaaacggc gacttttccc acaaaccggc
     2761 cgcaacatca cgctgatagg caggcagatt ccgcattaac gcaatcatca gcataaaggc
     2821 gtgttccgca accgattcgt ttccgtaggc gcggacattg cataccgcaa cgcccgccgc
     2881 ctccgccgcc ccaatatcga cattgttcac accggtcgca ctgacggcaa tcagctccaa
     2941 ctgcggatta gccgcaataa tgtcggcgga aatcattacc ttattggtaa tgacaacatg
     3001 tgcgtcgtgc acccgttctg ccgtttcatc cgcacctgtc gtaccgtaaa ctgcgagctc
     3061 gtgcggaaaa tcaaaatgaa aaaccctgcc gggcaaagta tccgcatcca gaacgactat
     3121 ctttttgtga ttcatcattt ctatccttac ttaaaaattg atttatttac actaacagta
     3181 aatagcaaat ttaaaccggc acggcctgat gataatcttt atcatctctc cgtaaatttt
     3241 caattcggga gaggatgatg cttaaaaccc tgacttttgc cgcactgcat ttcaccaccg
     3301 ccctcagtgt aacctatgtg ctgacgggca gtataggcgt atctggcgcg gtcgcgctgg
     3361 tcgaacccct aatcaacacc gtcgttttct atagtggatt aacaaaaacc agtacggcgt
     3421 tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgc cctgattttt
     3481 gttaatccac tatatttcca tgaaaaagca tggaaccgtt acgaaaaaaa caaaacgggc
     3541 aaacaacaac tgctgttccc gctgcaccgg tgcggctgac gattggcggt atttgacttc
     3601 gccggacggt atgttaccct tgccgttttg accaacaagc aacgaaagga cagatatggg
     3661 cagcctgatt attgaagatt tgcaggaagg cttcggaaaa gaagcagtta aaggcaaaga
     3721 gattaccgtg cattacacag gttggctgga aaacggcacc aaattcgact ccagcctcga
     3781 ccgccgccag ccgctgacca ttacgctcgg tgtcggacaa gtcatcaaag gctgggacga
     3841 aggcttcggc ggcatgaagg aaggcggcaa acgcaagctg accatccctt cggaaatggg
     3901 ctacggcgca cacggcgcgg gcggcgtgat tcctccgcac gccactttga tatttgaagt
     3961 cgagctgctg aaagtgtacg aataaagctg cccgggcaat accgtctgac ataatggctt
     4021 caagcaacgt aaacaacgaa atcaaagaca aaaaactctc cctgtgggcc aagcgtcaag
     4081 acggttcggt aaaatggttc tgcggacagc cggttacgcg caccggcacc ggcaccaact
     4141 ccgacgacgt taccgccgac agcgacaaga agatcga
//
LOCUS       DAXIEB010000136         4136 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000136 DAXIEB010000000
VERSION     DAXIEB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.136"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..890
                     /locus_tag="ACFOA3_001874"
     CDS             <1..890
                     /locus_tag="ACFOA3_001874"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6898825.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
     gene            complement(2115..3995)
                     /locus_tag="ACFOA3_001875"
     CDS             complement(2115..3995)
                     /locus_tag="ACFOA3_001875"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6898826.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFTE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQLLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTSFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSE
                     FNTGEPTCKIEAVSIAENLRIQTIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFAIAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     SAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGKTAGN
                     AV"
BASE COUNT          952 a         1051 c         1171 g          962 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaattccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctagcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctggaaacg aaaagcaaca
     1021 ggaatttatc gggaatggct ggagtttaaa ggactggatt cccgctttcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cctctatgcc gtcattcccg cgaaagcggg aatctaggac gtagaatcta
     1321 aagaaaccgt tttatccgat aagtttctgt accgaagaat ctggattccc gcctgcgcgg
     1381 gaatgacggc gtataagttc ccgtgcggac agacctatat ttccacctgc gcgggaatga
     1441 cgattcagaa gttgcccgaa acctaaaaaa ctgaaaccga acgagccgga tttccgcttt
     1501 cgcgggaatg acggcgtata agttcccgtg cggacagacc tatattccca cctgcgcggg
     1561 aatgacgatt cagaagttac acgaaactca aaaaaaacga aaccgaacga accggattcc
     1621 cgcttttacg ggaatgacgg gattttgggt tgtggtaatt tatcgggaaa acggaaaccc
     1681 ctatgccgtc attcccgcgc aggcgggaat ctaggacgta gaatctaaag aaaccgtttt
     1741 atccgataag tttctgtacc gaagaatctg gatttccgct ttcgcgggaa tgacggcgta
     1801 taagttcccg tgcggacaga cctatattcc cacctgcgcg ggaatgacga ttcagaagtt
     1861 gcccgaaacc aaaaaactga agccgaacgg tctggattcc cgctttcgcg ggaatgacgg
     1921 catatcagtt tctgtgcgga taaacctaga ttcccatctg cgtgggaatg acgggatttt
     1981 agattgcggg tatttatcgg gaatggcggt ttggaagttc actgaagcgc aaaaacaacg
     2041 gaaacccaaa aaaccggatt cccgattgtg ggaatgacgg gattttgggt cgtgggaatt
     2101 tgccgcacac ggcttcagac ggcattgcct gccgttttgc ctgcgggtgc gactgccttg
     2161 atgtagttga gcgagacaaa ctgcttctcg gcatctaatt cggtgatttt gaacaatgcc
     2221 tgtgatttgg gcagcgcgtc aaacggaata ccggttgcgc gtgttactag cggcagacct
     2281 tcgatgcgga cgaggtcttc tttcagaatc gtcgccgtca gttcgctgat gccttgctgt
     2341 tgcagataga cgagactcca gtaggcttcc atttggcgtt ggaagtcggc gtaggcggta
     2401 taggcggcat caaagtcgcg cagcgcggcg aagagttcgg catcgctgtt ctgatacagc
     2461 ggctcggcgg agtcgtcgat caggctgatc agctgctttt ggttgatgta gtcggcggcg
     2521 cggcgcagcg gcgaagtgaa ccagccgtaa tgctgcacgc ccatgccgat atgcggctcg
     2581 gatttggtgc tcatgcgcac ttttccggtg ggttggacgc ggaagaggcc gggcaggtcg
     2641 ttgtcatgga gcatttgtgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc
     2701 gtatcgatgg gcgaaccgcg ttcgcggcgg acgacggata ccttgccttc ctcatccaat
     2761 tcgatgctgt aatcgtattg cggcgcgcgg tcgggttcgt atttgccgcg tgctttttgc
     2821 agggcgatgg cgaattgata gaaccaaatc aagtcttgat ggtgggcgaa catcatttcg
     2881 cccgcttcgt ccaagccggt ttcggcgttg aaatgcggct cgatggtttg gatgcgtagg
     2941 ttttcggcga tgctgacggc ttcgattttg caggtcggtt cgccggtgtt gaactcgctg
     3001 tccacatcaa aataaatgct gacggcggga cggtacgcgc ccgcatcaag gctgaacgcg
     3061 gctatccagt tttcgggcag catggtgatt ttgccgccgg ggaaatagac cgtactcagg
     3121 cgttccatga tgtttttttc cattttgtcg cccggtttaa cggcaagtga cggcgcggcg
     3181 atgtggatgc cgacgcgttt cgtgccgttg cccaagtcgg tcaggctcaa ggcatcgtcc
     3241 acttcggtgg tcgattcgtc gtcaatggaa aaggcggtaa cgtcggcctt gggcaagtcg
     3301 ggcatttcag gaagcggaag gtcggggaag cttgttcctt tggggaagta tttgatttca
     3361 aaaccgtctt gcaggtattg gggaatggac gtaatgccgc ctgttttttt cgccaattcg
     3421 taggcggaag ttttcagcgc gtcggcagct ttggtaaagg ctttgtaggt cagcaactgc
     3481 ttgtcgggtg cgtgtaagat ggttttcaaa tccgccgcga tttcagacgg catctcgccg
     3541 cgttttaagg cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg
     3601 gcggcaagcg cttgttttaa ggtttcttcg ggcgcggctt tgaacacgcc tttggctttt
     3661 ttgtagaaat acatcggcgc ggcgtaaagt gcaatcaaag tcgccgccag ctcggttttg
     3721 gtcggcgcgt gtccgtaata ttcttcggcg atggcttcgg cggtaaattc ttcttcgccg
     3781 catacctccc acaataaatc ggtgtcgatg tccgccgcct gcgcctgcgc gttctccaaa
     3841 aacgccgcca tatcgccgtc gaactcggta aagacgttgt tcgccttcac tttggtgcgt
     3901 ttgccgtgtg gggtatcgac ttggtaggtg gcatcgtttt tttggatgat ggcggcgatt
     3961 ttgaattggc cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc
     4021 aagcgggcga ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt
     4081 ggcgcgtctt tgcggtgctt gcgttatagt ggattaacaa aaaccagtac agcgtt
//
LOCUS       DAXIEB010000137         4129 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000137 DAXIEB010000000
VERSION     DAXIEB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.137"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(179..1429)
                     /gene="glyA"
                     /locus_tag="ACFOA3_001876"
     CDS             complement(179..1429)
                     /gene="glyA"
                     /locus_tag="ACFOA3_001876"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6898827.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVSAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1495..1707)
                     /locus_tag="ACFOA3_001877"
     CDS             complement(1495..1707)
                     /locus_tag="ACFOA3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898828.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGDETLKTS
                     MLNKLPMLNKLPDNAKENLRIMGYLP"
     gene            complement(1928..3703)
                     /gene="ggt"
                     /locus_tag="ACFOA3_001878"
     CDS             complement(1928..3703)
                     /gene="ggt"
                     /locus_tag="ACFOA3_001878"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6898829.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKVLHAGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3871..4129)
                     /locus_tag="ACFOA3_001879"
                     /pseudo
     CDS             complement(3871..4129)
                     /locus_tag="ACFOA3_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT         1037 a          968 c          971 g         1153 t
ORIGIN      
        1 gatatgtttg gcaatcatgg cttgcgcggt ttgccggaag aaggtgctca tggaaaatcc
       61 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tttccgcctg
      121 aaacattatg agatttcagg cggcattgga ttgcttagcg gaatattttt aaaaaggctt
      181 acgcgccgta aacggggtat ttattgcaca aagcagttac ttgttcgcgg actttggcga
      241 ggttggcttc gtcttcgggg ttagacaata cgtcggcaac caagttcgcc aatacgcgcg
      301 cgtcggcttc gttaaaaccg cgtgtggtca tggcggcgga accgatgcgg atgccggagg
      361 taacgaaggg tttttccgga tcattcggga tggcgttttt gttgacagtg atgtgcgctt
      421 tgcccaaagc ggcttcggcg gctttgccgg taattttcat cggttgcagg tcgacgagga
      481 aaacgtggct ttcggtgcgg ccggaaacga tgcgcaaacc gcgtttgacc agttcttccg
      541 ccatggcggc agcattgatt ttcacttgtt ttgcgtattg tttgaactcg ggttgcaacg
      601 cttctttaaa cgctacggct ttggcggcga tgacgtgcat cagcggaccg ccttgcaggc
      661 tcgggaagat ggaagagttc agtgcttttt cgtgtgtgtt gtcgcggcac agaatcacgc
      721 cgccgcgagg gccgcgtagg gttttgtggg tggtggtggt cacgaagtcg cagaatggca
      781 cggggttggg atattcgcca ccggcaatca gtccggcata gtgcgccata tcgacaaaga
      841 ggtatgcgct gactttatcg gcgatttcgc ggaattttgc ccagtcgatt tgcaacgcgt
      901 aggcagacgc acccgccaca atcattttgg gtttgtgttc gagcgcgagg cgttcgactt
      961 cggcataatc gagcacttcg ttttcatcca aaccataagt aacagcgttg tagagtttgc
     1021 ctgagatatt aacgctcgcg ccgtgggtca ggtggccgcc gtgcgccaga gacataccca
     1081 aaatggtgtc gcctggtttt aaaacggaag cgtacacggc ttggttggct tgcgagccgg
     1141 agtgcggttg gacgttggcg tattgcgcgc cgaacagttt tttgacgcgg tcaatcgcca
     1201 attgttcgac aatatcgacg tattcgcagc cgccgtagta gcgtttgcca ggatagcctt
     1261 cggcgtattt gttggtcaat tgcgaacctt gcgcgtccat tacggcgcag ctaacgtagt
     1321 tttcggaagc aatcagctcg acgtggtctt gctggcgttg gtcttcttgg gcaatggctg
     1381 ctgccaaatc ggggtcgtat tgtgcgaggg taacgctttt tgaaaacatg ttctcggctc
     1441 ctttgtgtaa tcagggtatc atgagtgttt tttgtataaa aaatacttca atacctaagg
     1501 tagatagccc ataatgcgta aattttcttt ggcattatca ggtaatttat ttaacatggg
     1561 taatttattt aacatgctgg tttttagcgt ctcatcacct ttatttagta caagactaat
     1621 caaaagcaat acattaaatg gtaaattttc ggcagtttgt gcaaaatcaa tcaaataacc
     1681 agcaccaacc acatctttat cagccataac ccgctgatac accacttgct aagacaactc
     1741 tcctgcccca ccatgtctta aaatcagatg aatggcttgt aatgctgtgt ttggtgtctc
     1801 acaaattgcc aaaatctgat cagaagcttg cttgatggct tgtatttttg tttgattact
     1861 cacaatttcc ccctatttta ataattaact taaatgcggt caaattcaca aaatacaagc
     1921 ttcacctcta atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc
     1981 tgcttggctc gataataatg gcttgaacac ctgaatttag ctcacgcaca tcagtcttat
     2041 agcccaaatc atttaatgct tgttgccact ggacggcggt tgtacccgtt tctagttcat
     2101 agttaccaaa gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc
     2161 agtcactatg tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg
     2221 agccgattgc catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc
     2281 gtggtctctt gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa
     2341 aactaaagtc tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa
     2401 acgcattttc aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata
     2461 tatgactggt agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc
     2521 ctgcagatac gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta
     2581 ggtagtcttt agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat
     2641 atacatcacg atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag
     2701 catcataccc cacctgattg ggtgaaaatt catttaaaat ccccaaaatc tgacccacag
     2761 caatcccacc tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac
     2821 aaacaggcgg gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg
     2881 ggttatcctt agcattttgg acaactgaaa cgatattttg ggcatattta ccagcatgca
     2941 gaacttttgc accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca
     3001 gcaagctgcc tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat
     3061 agcgtgccaa atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa
     3121 agccttgttt tgccaagcgg atcggcgtat caaataattt tccccaaggc aatacaccgt
     3181 atcgctgatg tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac
     3241 cgaccaccgc ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg
     3301 tcgcacgcat cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat
     3361 cccaatacaa cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta
     3421 gtgtcgtctg caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat
     3481 agccagcttg tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct
     3541 gcttttgttc ggtcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtaatga
     3601 cagcagaatg agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg
     3661 tcatagacat tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg
     3721 agtttgattg atgattcata caaggcatgc tgattattgt atttaatatg actaaataat
     3781 tcaatccaaa ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt
     3841 ttttgatatt tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag
     3901 tggttttagc tcacctgatt ggtacatttg tgataatatc actaccaccg attaactcac
     3961 cattaaccca aagctgtgga aaggttggcc gattggcgat gagtggtaga gtactgcgaa
     4021 tttctgggtt ttctaggata ttaacaaaag caaagggtct gccaattggg tcagcacctc
     4081 tactgcacgc gctgaaaatc cacattgggg aaactggggc gtgcctttc
//
LOCUS       DAXIEB010000138         4120 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000138 DAXIEB010000000
VERSION     DAXIEB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.138"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(233..1426)
                     /locus_tag="ACFOA3_001880"
     CDS             complement(233..1426)
                     /locus_tag="ACFOA3_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6898830.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLSQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1423..2406)
                     /gene="patB"
                     /locus_tag="ACFOA3_001881"
     CDS             complement(1423..2406)
                     /gene="patB"
                     /locus_tag="ACFOA3_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6898831.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETLPTAQDSGLEDMPSEAAASEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     HTDSVNVNGKSVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2417..3853)
                     /locus_tag="ACFOA3_001882"
     CDS             complement(2417..3853)
                     /locus_tag="ACFOA3_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6898832.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFAAVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(3983..>4120)
                     /locus_tag="ACFOA3_001883"
     CDS             complement(3983..>4120)
                     /locus_tag="ACFOA3_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6898833.1"
                     /translation="NVGIGGGALLGHWVTQYVGISRIGIAGMLAAAAGLCLNLNRHIQ
                     T"
BASE COUNT          841 a         1047 c         1346 g          886 t
ORIGIN      
        1 cgtctgaaaa cggaaggcgg cagtttatca tctttgcctt cagacggcat atcaaacgcc
       61 gcctttgcgc cgtatgccgg aggttcgccc ccgtccttcc gatgcttgag gcgaatgccg
      121 gttgcgtccg gcaaaccgtg cgcggggtgg ctatgccgtc tgaagcaata tgaaggtatg
      181 cgtttcagac ggcatccccc tgttgcggaa ataccgtccg cctcctgtcc gattattgcc
      241 tgattgcagc ggcgcggacg agttcttcga ggctgtcggc gagcatttcc gccgcgcgcc
      301 ggtagccttt ggcggagaag tgtacgccgt ctttggcggc ccatccttgg ttgagccagt
      361 ttttcatgct gcatatgccg cccatggcgt tttgccaaga ccagaacatg gtttgtccct
      421 gccgggcgac gcgccgctgc atctgttgga cttcggtcag gcggacgggg cgtgtgccgc
      481 atacgccgag cgtgtttttc agggattcgg gtgcgccgat gatgaggatg ccggcggcag
      541 gcaggctgtc gtggatttgg cggacggtat ccagccattt ttgttcggta tcggcaatgt
      601 cgatgttgtt gttgaaggct tcgttggtgc cgtaggaaag gataaccaaa tcggcgccgg
      661 tttgggcaag gtcgttcata cggtcggcac gccatttcga ccattgggac aattgtgcgc
      721 cgttgatgcc catcgcggaa acggtaatgc cgccggcggg gttttcgatg ttgatgaagc
      781 cgatgtccca cggcatttcg gtgtgtatgg tcaggggcag tgccgcgccc gtatccagta
      841 cctgccagcc gccgccgttg gcggagacgg tgttgccgtt gacggtcagg gtttgttcgg
      901 cgagcagggg tttggcaaac agggaaatgc gctgtttgcc ggtcttgccg tccgatgcgg
      961 tcagggtcat gctgccgccg ctgccggtgt gggcgaggat gccgccgagc gggaagtctc
     1021 cgctattgtt cctgctggtg aggctttgcc agttaccgtt gtgccggacg gccgccatgc
     1081 gctgcccttt gacgttggcg gggtaaaccc agcctatgcc gccgtcgccc caagtttttt
     1141 gcaggcgttt gcgcaggctg tcggtaaaga agtcgccggc ggtatgcgag tcgccgattt
     1201 gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga tcggagtttt ttcatccaag
     1261 gcgaggcggc ggcgttgccg tagtcggtca agagcccgtt tgtatcggtg tagggggcgg
     1321 acggggaaac ggtaacggtg tcgaggctga cggaggcgac gggcagggct tctgcctgcg
     1381 tggcggcgaa cagggcggaa aatgcgatga ggtgtttggg gttcatggct gtgtacttga
     1441 tggttgcgta cttggttcaa aaacgatttt ttccattatt tttgccgcca gcagtttttg
     1501 tccttcggcg gtaaagtgta tgccgtcctt gctgcggtag cggacggatt tgccgttgac
     1561 gttgacggaa tcggtgtggc ggtcttcccc gccgctcagg gtgtgcgcgg tgggaatcag
     1621 gatgattttg ccttccaagt gttccgaaag cagtttgtcg aggtagcgca tctgcccgtc
     1681 gagcttggct tttttcatgt aggggatgcc gagccagacg acttggacgt ggtgcgcgcg
     1741 ggcggcttcg aggatgcggt cgacgcgttt caggtattct tgcgcccatt cgtcggaagc
     1801 gaatttgagg tagcgtttgc cgacgggaaa atcccacggg tcgttcggac cgaggaagac
     1861 ggcgagcacg ctgatttcgg ggtgtttttg cagggtttct tcaatcgttt tcggccagtc
     1921 gaagaatgag gggtaggaca gccccgtgct ttgtttgctg aggttgacgg attcgatgcc
     1981 gtattgctgt ttcaggcttt tttgcacgaa gggtgcaacg ccctgcatca gcgaatcgcc
     2041 ggcgaaaaag actttgtcgc cgctgcggac ggcggcggtg tcggtgtctt gtttccattc
     2101 tgtttcaccg gtttgaggga cggcttcgga tgcggcggct tcagacggca tatcttccaa
     2161 accgctgtct tgggccgtcg gcagtgtttc gccggacagg aaggctttga tgccgtctga
     2221 aagggcgtag gcgttttctt gcaacgccac accgctccgc caccatccgt aggcggcaag
     2281 cggttcgagc ggactgttgc ggtggtaggt ctgctgccag taggcgttga tggggttttg
     2341 gctgaaccac acggcaatca gggcagacat cagtatggag gcgaaaaggg aaagaaagtt
     2401 tttcatgtcc aaacccttaa aaattggcat aaataaagcc gggtatcccc gagggggcca
     2461 atacgataat cagcagcagg acggcggaaa cggggataaa ccacagccac atcgggattt
     2521 tttccaaacc tttgaccgcg ccgtcgaaag cgcgttgcag gtaagggtag agcagcatca
     2581 cggctgcaaa cgaggcaagc agcagcatat ccgcctgttg cggcgcattc cagccgttgg
     2641 cattggcaaa gagggcattg aaaaccgcgc cggcatcgtc ggggtttgcg gtattgaaga
     2701 cgacgaaggt caggcagacg aaatggaagg taacaaacca cgagagcggc gcaaggtatt
     2761 tcagacggca tagtgcgttg cgtccgaaat agcggtcgcc cgtgttgagc agcaccagcg
     2821 ccgtgccgtg tagtgcaccc caaatgagga agttccagcc gtagccgtgc cagatgcctg
     2881 agagcaccat tgccgccatc aggttgaact gtgtccgcaa aaagcctttt ttgctgccgc
     2941 ccaaggggat gtagatgtag tcgcgtatcc aggtggaaag gctgatgtgc catttgtccc
     3001 aaaatgcgcg gatattcgcg gcacgaagcg gtgcggagaa gtttttgggc agccggaagc
     3061 ccagcagcat tgccattccg ataaccaagt cggaatatcc ggaaaagtct aaaaagagtt
     3121 ggaaggtata gccgtacacg cccgccaata cgccccagcc gtcgaattgg gcgggatttt
     3181 caaatacggg cgatacccag ttttctgcga gcatccccgc cagccaccat tttttggcaa
     3241 tacccagcag aatcagggaa acggcgagtg cggggcggac gggtgagcgt ggttggtggg
     3301 tacggatttg cgccaatgcg cccgcctgct cgccgtccgc gcttttgaat gcggcggcgc
     3361 ggataatcgg gccggaggta acggtgggga aaaaactcag gtgcagcagc agttcgtgcc
     3421 actcgaaacg cgcggcgtgc ggcgcacgga agcagtaaac caaataggcg attgactgga
     3481 aggtgtaata cgaaagcccc agcggcatca ggatgtcgat tgcgccgcct tgtccggcat
     3541 attgtgcaat cagcgggcgg aaaaaatcga aatatttgaa aaagcccaag acggtcagcg
     3601 agacggcaat gccgcacccc agccaaaagc ggcgcgtatt ttcgcgatcg gaacggagca
     3661 gttcgcccaa aaggtacacg caggaggaat aaaggacgac gattgccgca aatacggggc
     3721 cgatatggta gagccagccc ataccggcag ccaaaagcag caggttttgg acggacgggt
     3781 atttcgccaa gccccagtaa atcggcagga aggcgagaaa gaacagtgcg aactcgacag
     3841 acagcagcgg cataaaccat ccttggaata agaaaatagg cgttaaaaca cggcaaagcc
     3901 cgttatttta agcaggggcg gatatttctt caatcaaaat gccgtctgaa gggcgggagg
     3961 ggcttcagac ggcatcgcgc ggtcaggttt ggatatggcg gttcaagttc aggcacagcc
     4021 cggccgccgc cgcaagcata ccggcgatgc cgatacggga aatgccgaca tattgcgtaa
     4081 cccaatgccc cagcagcgcg ccgccgccga taccgacgtt
//
LOCUS       DAXIEB010000139         3948 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000139 DAXIEB010000000
VERSION     DAXIEB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.139"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..513
                     /gene="mafB"
                     /locus_tag="ACFOA3_001884"
     CDS             <1..513
                     /gene="mafB"
                     /locus_tag="ACFOA3_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6898834.1"
                     /translation="NSARQWADAHPNITATAQTALSAAEAAGTVWRGKKVELNPTKWD
                     WVKNTGYKKPAARPMQTVDGEMAGGNKPLESKNTVTTNNFFENTDYTEKVLRQASNGD
                     YHGFPQSVDAFSENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRL
                     FVPNQQLPEK"
     gene            529..831
                     /gene="imm40"
                     /locus_tag="ACFOA3_001885"
     CDS             529..831
                     /gene="imm40"
                     /locus_tag="ACFOA3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC6898835.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            915..1253
                     /locus_tag="ACFOA3_001886"
                     /pseudo
     CDS             915..1253
                     /locus_tag="ACFOA3_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1233..1673
                     /locus_tag="ACFOA3_001887"
     CDS             1233..1673
                     /locus_tag="ACFOA3_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB"
                     /protein_id="HFC6898836.1"
                     /translation="MASGNKPPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQD
                     RIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHR
                     RVRYDINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            1639..1845
                     /locus_tag="ACFOA3_001888"
     CDS             1639..1845
                     /locus_tag="ACFOA3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6898837.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            1866..2084
                     /locus_tag="ACFOA3_001889"
     CDS             1866..2084
                     /locus_tag="ACFOA3_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898838.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            2081..2440
                     /locus_tag="ACFOA3_001890"
     CDS             2081..2440
                     /locus_tag="ACFOA3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6898839.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            2516..2728
                     /locus_tag="ACFOA3_001891"
     CDS             2516..2728
                     /locus_tag="ACFOA3_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898840.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            2815..2931
                     /locus_tag="ACFOA3_001892"
                     /pseudo
     CDS             2815..2931
                     /locus_tag="ACFOA3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            2993..3349
                     /locus_tag="ACFOA3_001893"
     CDS             2993..3349
                     /locus_tag="ACFOA3_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6898841.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            3397..3525
                     /locus_tag="ACFOA3_001894"
     CDS             3397..3525
                     /locus_tag="ACFOA3_001894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898842.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            3522..3896
                     /locus_tag="ACFOA3_001895"
     CDS             3522..3896
                     /locus_tag="ACFOA3_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6898843.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT         1435 a          596 c          757 g         1160 t
ORIGIN      
        1 aattccgcca gacaatgggc tgatgcccat ccgaacatta cagctactgc ccaaactgcc
       61 ctttccgcag cagaggccgc aggtacggtt tggcgcggta aaaaagtaga acttaacccg
      121 accaaatggg attgggttaa aaataccggc tataaaaaac ctgctgcccg ccctatgcag
      181 actgtagacg gggaaatggc tggtgggaat aaaccgctgg aatcaaaaaa tactgtaaca
      241 acgaataatt tttttgaaaa taccgattat acagagaaag tattaaggca agcatcgaat
      301 ggtgattatc atggttttcc gcaatcagta gatgcttttt ctgaaaatgg aacagtaata
      361 caaatagttg gcggagataa tattgtaagg cataaacttt atatccctgg atcttataaa
      421 ggaaaagatg gaaattttga atatatcaga gaagcagatg gaaaaattaa tcatagatta
      481 tttgtcccta accagcaact tcctgaaaaa tagtagatag agattaacat gttaaatgaa
      541 atttttgaaa tttattcgag acaaggggaa tctttgatag gaattggaat tagagaagcc
      601 gcattacccg tccctattgc aatagatata ttaaatttat ttatcaatga gagaatactt
      661 gtattggggg gagatattta tatcaagaaa gataattatt tttatcaaac atatgataat
      721 tggtattacg agggaagtaa tttatttaac agtatcgaca aagcaatgca ttatttatct
      781 caaataaaat tagagaatgc atacgtatct tttgtgttga aatttatcta acaaaggaag
      841 cacaagaata gatttatagt aaaacatcaa gatgttgaaa atgctgggtt ttaatccaac
      901 ctacactgac cggctcagat acagccgctc agcagactct acaaggtatt aatgatttag
      961 gaaatttaag tccggaagca caacttgctg ccgcgagcct attacaggac agtgcttttg
     1021 cggtaaaaga cggtatcaat tccgccagac aatgggctga tgcccatccg aacattacag
     1081 ctactgccca aactgccctt gccgtagcag aggccgcagg tacggtttgg agaggtaaaa
     1141 aagtagaact taacccgact aaatgggatt gggttaaaaa taccggctat aaaaaacctg
     1201 ctgcccgcca tatgcagact ttagatgggg agatggcaag tgggaataag ccaccgaaac
     1261 caagtacgca gcaacaccct acacactctg ataacaatat cggcttacct gcctcatatg
     1321 ttaaacctga tacatctatt tctccgacag gaacaattca agaccgcatc agatggacaa
     1381 agtccaagtt tcctactgag aaatctttaa atggacattt caaagctcat ggaaaagaat
     1441 ttggcgatat aaccattgaa gactaccaaa aaatggcgtc tgatttgtta tcaaaacaga
     1501 catcggacaa gatattaggt tatcagacgg aacatagacg agtgcgctat gatatcaata
     1561 acaatatcta tgttttggcc aatccaaaaa cattcaaaat caaaacaatg tttaaaccaa
     1621 acttaggaaa gaagtattat gatggagaat tcaaaaaaga catgggaaat tgacggagaa
     1681 atatggctac attgtcctgt ttgcggaact gaagttatgg actatgatat ctgtgacgtt
     1741 tgtcagtggc aaaatacagg agaaactaat atagatggtg gccctaatga aatgacactt
     1801 gcggaggcga aagaagctta cgcaaaaggc ttaccaatca gataaataag cacctagaga
     1861 aatcaatgat gacggaatcc catggttacc tatcaaataa tttacctgtt aaaatcataa
     1921 atgatattat ttatgcaacc cagttagtcg aagatttggt tttaggaaaa ataaaaattg
     1981 ttgatttttt aaaatcatat aacaattttt attgttggct tggttttgat gagttgcctc
     2041 aatctgagaa aataaaattc ctaagctatc tcaatatatt atgagattaa aattaaaaaa
     2101 ttatgattgt tctgactttg atttggaaga tttcccacaa gacaagttag aaaatttttg
     2161 tatacttttg acattatcaa taggttttga tgaaagtaat ggtgctgatt atttttatgt
     2221 ttatatatat agcaccgaat ggttactttc aaatatacat agaccaatgt cattaaaaaa
     2281 ctcaattgtt acaaaccgtt ttaatattga acacatatta aaattgataa atgatatatt
     2341 ggaaatatgt aattcaacgt ctgaagataa gtctatatca aatttggcaa aatattttga
     2401 ctgggaattt gacgattata atctaaatat ccaagactaa agatggtgga cagcatttag
     2461 accaaaaaca ggcaaacggt attttgatga cgaattaaaa taaaaggaat tattcatgaa
     2521 attaattaat tttgaagaga gttcagttaa aattagtctc gataaagatg agttgtatat
     2581 tattcaagca attgtaggag aaatttattc aggcgtatgc gtggattgta gagattttga
     2641 gataattcat ggtgttgaaa aaataaagtt ctttctttgg ataaagaact taaaaaaata
     2701 tatgatactt gggataaatg ttagctaggt aaggtgggca cttgttgccc accttttgcc
     2761 gtatattaaa aatccttttc aggctggaac tgggtgggca tcaatgccaa ccctaattgt
     2821 gctgatgaag caggaaaact gatatgggaa aacgacccca ataaaaattg gtgggctaac
     2881 cgtatggatg atattcgcgg catcatccaa ggtgcggtta atcctttaat ttacaaggta
     2941 agcaagcaaa agattattta caacaacaaa ctcatatcag gaacttagac aaatgaatga
     3001 acacaacctg ttaattttct gtttaaaaga caatgtttca attagtgaat atactgaaat
     3061 gattgattgg gcttataaaa acattcaatc tgaaacagtt gtagaaatta cggaaaatca
     3121 aattattgaa tatcaaaatc gtggattatg gagacttgtt tctgaaatta ccgataattg
     3181 gttatttgga ccaagtgagg gggattggct aatagataag gaaagtattt tggctgtaaa
     3241 agaaaaatta caaaattcag atttttctac agagccctta gtgaaaaata ttattcatgt
     3301 acttgaatat gctataaaaa atgaaaaaac agtaattttt catttttgag actaatccaa
     3361 tttttagtaa tattgatgca gagcaagcag cattagatgc cgcaaacatg gggagaagct
     3421 attcaattta gaattaaaaa acaaattgaa aatgaactag caccaccaaa ttggtctacc
     3481 cagtttccta atggtagtat ttatgatcct aaggtaacga aatgattatt caaaatgaat
     3541 ttaatttata tcctagtaat atgcttcctg aaaggttttg ttatcctgaa aagtatgttc
     3601 gtatctctaa cgatacatct ttaatacctt atattcagcc acataatttt cactggtggt
     3661 ttgagaatta tggaacagaa ggggcagaag tagcttatat atttagaaat tctatcctgc
     3721 ctgatttaaa tcttatccca ttcgctagta atggagaatg ggaagcttat tttgatggta
     3781 atgatgtaac aggaaatcct agggttattg tcattaattt agataatata gaaaaccatg
     3841 aattttttaa tagttttgaa gagtggcttg aattagcaat taaggatact tggtaagcag
     3901 ctatctataa aagagatggg accatcctgg ataactagga taaactcg
//
LOCUS       DAXIEB010000140         3872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000140 DAXIEB010000000
VERSION     DAXIEB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.140"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFOA3_001896"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFOA3_001896"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6898844.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFOA3_001897"
     CDS             1439..1924
                     /locus_tag="ACFOA3_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6898845.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFOA3_001898"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFOA3_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6898846.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGYGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDGLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACESDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          796 a          944 c         1075 g         1057 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccat ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacgg ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggcg tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgctttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgcga
     2161 aatgaccatc acgctgccgg caaattccaa caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcaagg tcgttagacg gttcatccag cagcaacaca ttgccgccgc ccaacaaggt
     2281 ttttgccaag tgcagacgac cgcgttcgcc gccagacaat tgacctgcaa ttttgctttg
     2341 gtcgctgcct ttgaagttga agcgtcccaa atattggcgg gcgggaattt caaactgacc
     2401 aacctgcaaa atatcgcggc cttcggcgat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaggctcat tttcacggtt tgtccgattt tcacctcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagt gtcgatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgcccgc aggtactttg aagctcaaac cgtcaatcag
     2641 cactttatcg ccaaacgatt tggaaacatt cacaaattca atcacttcat tacccaaacg
     2701 ctcggcaaca gggataaaga tttcctgcgt ttcattgcgt ttttggtatt catagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatagccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccattccac
     3061 cgattccgca tccaagtggt tggttggctc gtccagcagc agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacagattgc cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcctgtggac gaacccgccg caataattgc ttccaaacgg ccctgttctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcttccaagc gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcacgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacaccag ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaggaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 agttttctcg tttcggtcgg acaaatttca gacggcacat tttaaccgat aatttgattt
     3721 aagccagttt atccgcgaac cggtattgcc aaaatcgggc aggattcata aaatccgtct
     3781 atccctttga aattatacaa acaaaaaaaa taataatgat aggtgatcgc cgcctcggca
     3841 accattttgg attttccaaa gcaaatatag tg
//
LOCUS       DAXIEB010000141         3794 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000141 DAXIEB010000000
VERSION     DAXIEB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.141"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(148..804)
                     /locus_tag="ACFOA3_001899"
     CDS             complement(148..804)
                     /locus_tag="ACFOA3_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6898847.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            868..1758
                     /locus_tag="ACFOA3_001900"
     CDS             868..1758
                     /locus_tag="ACFOA3_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6898848.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKLVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADIIKESAGIAAGL
                     LAGEIAPAARWLRNRITEST"
     gene            1876..3687
                     /gene="typA"
                     /locus_tag="ACFOA3_001901"
     CDS             1876..3687
                     /gene="typA"
                     /locus_tag="ACFOA3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6898849.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          900 a         1009 c         1121 g          764 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggtttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggctttgccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacagcag cgatgtggac tgcggaaaaa acggcgagca gcttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc cgtatgttcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc ccggtgccga cttggaacga
      481 cacggcggcc agaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggctggat
      541 gtgttcggga ataccgtttt tcagatagcc gcgtatgcct gcccagcctt ggacgaaacg
      601 ggaaaaacgg gcggtatcgc tgccccaaat gccccagcag aggcgaaata cgagcaggaa
      661 aaggacgaac agcccgacgc gcgtgtgcca ttgcagcata tcgccgccgg ctttcgcgct
      721 ataccacata aagggcaggg acgcggcaag cagccagtgg aaaaggcggg tggggaggtc
      781 ccagactttg gttttgtttt tcataatcgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttatttt aaccgattgg aggggcaatg tttcccgttt ttcatctttc aggcgagagc
      901 cgccgccgga tgcttcagac ggcattgcgt tttccccatg ttttcaaagc ccgtgcggaa
      961 gattcgcaca aagggacttt cggcacgctc gccgtagtcg gcggatcggc agggatgagc
     1021 ggcgcgcccg tattggcggc atcggcggca atgtatctcg gctgcggcaa agtgtgggcg
     1081 ggtttcaatc aggatacgct accttttgcc gttattgccg gttttcccga gattatgctg
     1141 gatacggcgg acagtttggc caaacgtcaa gatataaacg cctgggttgt cggttgtgga
     1201 ttgggtacag gtagggcggc ggtcggaacg cttgccggaa ttttgacgga acacacggac
     1261 aagctcgtcc ttttggatgc ggacgcgctg aacatattat caaccgatgc cgaaacccga
     1321 aatctggcac gcgggtgtaa aaacctgatt ttaacgccac accccgccga agccgcgcgc
     1381 ctgcttggaa cgacggttgc acaggttcag gcggatcgga cggcggcagt gaggaagata
     1441 ggggcaattt tcggcgcaac cgtggtttta aaggggcaca aaacattggt tgccgcgtcc
     1501 gatacggaaa tctatgtcaa cgaaagtggc aacgcgggat tggcaacggc gggcagtggc
     1561 gacgtattgg gcggcatcat cggcagtctg ctcgcacagg gcgtgccggt ttttgaagcc
     1621 gcctgcgcgg gcgcgtggct gcacggcgcg gcggcggata tcataaaaga atcggcaggc
     1681 attgcggcag ggctgttggc aggggaaatc gccccggcgg caaggtggct gcgtaaccgg
     1741 ataactgaaa gtacgtaatg aagatgccgt ctgaaaggca agggcttcag acggcatctt
     1801 catttcccaa atactgtccg gtaaagcgtg gtataatgcg cagttatctc ttacagtttt
     1861 tgaaaaacat taattatgaa acaaatccgc aacatcgcca tcatcgccca cgtcgaccac
     1921 ggcaaaacca cattggtcga ccaactgctg cgccaatccg gcacattccg cgccaaccag
     1981 caggttgacg agcgcgtgat ggacagcaac gacctcgaaa aagaacgcgg catcaccatc
     2041 ctcgccaaaa acaccgccat cgattacgaa ggctaccaca tcaatatcgt cgatacgccg
     2101 ggacacgccg acttcggcgg cgaagtagag cgcgttttgg ggatggtgga ctgcgtcgtc
     2161 ttgttggtgg acgcgcagga aggcccgatg ccgcaaaccc gtttcgtgac caaaaaagcc
     2221 ttggctttgg ggctgaaacc gattgtcgtc atcaataaaa tcgacaagcc gtctgcacgt
     2281 ccgagctggg ttatcgacca aactttcgag ctgttcgaca acttgggcgc gaccgacgag
     2341 cagttggatt tcccgattgt ttacgcttca ggtttgagcg gctttgccaa gctggaagaa
     2401 accgacgaga gcagcgatat gcgcccgctg ttcgacacca tcttaaaaca cacgcccgca
     2461 ccgagcggca gcgcggacga gccgctgcaa ctgcaaattt cccaactcga ctacgacaac
     2521 tacaccggcc gcctcggcat cggtcgtatc ttgaacggac gcatcaaacc cggccaaacc
     2581 gttgccgtca tgaaccacga gcagcaaatc gcccaaggcc gcatcaacca gcttttgggt
     2641 ttcaaaggct tggaacgcgt accgcttgaa gaagccgaag ccggcgacat cgtgattatt
     2701 tccggtatcg aagacatcgg catcggcgta accatcaccg acaaagacaa ccccaaaggc
     2761 ctgccgatgt tgagcgtgga cgaaccgacg ctgacgatgg actttatggt caacaccagc
     2821 ccgctggcgg gtacggaagg caaattcgta accagccgcc aaatccgcga ccgcctgcaa
     2881 aaagaattgc tgaccaacgt cgccctgcgc gtggaagaca ccgccgatgc cgacgtgttc
     2941 cgcgtatccg gacgcggcga gctgcacctg accattttgt tggaaaatat gcgccgcgaa
     3001 ggctacgaac tcgccgtcgg caaaccgcgc gtcgtgtacc gcgacatcga cggtcaaaaa
     3061 tgcgaacctt atgaaaacct gaccgtggac gtacccgacg acaaccaagg cgcggtaatg
     3121 gaagaactcg gccgccgccg tggcgaactg actaatatgg aaagcgacgg caacggacgc
     3181 acccgcctcg aataccatat tccagcgcgc ggcttgatcg gtttccaagg cgaattcatg
     3241 accttgacgc gcggtgtcgg actgatgagc cacgtgttcg acgattacgc gcccgtcaaa
     3301 cccgatatgc ccggccgcca caacggcgtg ctggtgtccc aagagcaggg cgaggcggtc
     3361 gcttacgcct tgtggaatct ggaagaccgc ggccgtatgt tcgtatcgcc caacgacaaa
     3421 atctacgaag gtatgattat cggcatccac agtcgcgaca acgatttggt ggtcaacccg
     3481 ctcaaaggca aaaaacttac caacatccgt gccagcggta ccgacgaagc cgttcgcctg
     3541 accacgccga ttaagctgac gctggaaggt gcggttgagt ttatcgacga tgacgaactc
     3601 gttgaaatca cgccgcaatc catccgtctg cgcaagcgtt acttgagcga attggaacgc
     3661 cgccgccact ttaaaaagct ggattgatgt ttactggatt aatgtttaaa tgatacgcga
     3721 tgccgtctga aaaatttcag acggcatttt ttattcggac gggctttgcg gcttcttgaa
     3781 gctgtttcag acgg
//
LOCUS       DAXIEB010000142         3750 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000142 DAXIEB010000000
VERSION     DAXIEB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.142"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..1770
                     /locus_tag="ACFOA3_001902"
     CDS             <1..1770
                     /locus_tag="ACFOA3_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6898850.1"
                     /translation="IKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVL
                     SAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEG
                     MTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATT
                     GVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAA
                     VTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNL
                     EANILAALVNTAHGEAASKIKGLDQHYVAHKIAHAVAGCAAAAANKGKCQDGAIGADV
                     GEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNA
                     VENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLD
                     WNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFG
                     KQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFV
                     LNMDGSLNQMKTGAAKGRKLNLK"
     gene            1779..2156
                     /locus_tag="ACFOA3_001903"
     CDS             1779..2156
                     /locus_tag="ACFOA3_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898851.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            2434..2808
                     /locus_tag="ACFOA3_001904"
     CDS             2434..2808
                     /locus_tag="ACFOA3_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898852.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2995..3750
                     /locus_tag="ACFOA3_001905"
                     /pseudo
     CDS             2995..3750
                     /locus_tag="ACFOA3_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
BASE COUNT         1193 a          793 c          905 g          859 t
ORIGIN      
        1 atcaaaagca gcatccacac agaaaccgtg agcagcagca aatctactct atggcaaaaa
       61 caggcaggac ggggcagtaa catcgaaacc ttgcaattgc cgagtttcac cggtcccgtt
      121 gcgcccgtac tgtccgcacc cggcggttac attgtcgata ttcccaaagg caatctgaaa
      181 acccaaatcg aaaccctcac caaacagccc gagtatgctt atttaaaaca gcttcaagtt
      241 gcgaaaaaca tcaactggaa tcaggtgcag cttgcttacg ataaatggga ctacaaacag
      301 gagggcatga cacccgcagc agcagctgtc gtcgttatcg tcgtaaccgt attgacctac
      361 ggcgcactgt ccgccccggc agccgccgga acggcgggcg cggcaggcgc aggagcggga
      421 ggagccgcag caggaacggc agccggaact ggagtagcag caggaacggc agccacaacc
      481 ggagtagcag caggcacatc agctgcagct atcaccacag ccgcaggcaa agccgcactg
      541 gccagtctcg ccagccaagc cgcagtttcc ctcatcaaca acaaaggaga cataaaccat
      601 accctgaaag aactgggcaa aagcagcacc gtcagacagg ccgccaccgc cgccgtaacc
      661 gcaggcgtac tgcagggcat aagcgggctg aacacccaag cagccgaagc cgtcagcaaa
      721 cattttcaca gtcccgcagc aggcaaactg accgctaacc tgatcaacag caccgctgcc
      781 gcaagtgtcc ataccgccat caacggcggc agcctgaaag acaatctgga ggcgaatatc
      841 cttgcggctt tggtaaatac cgcgcatggg gaggcggcga gtaagatcaa aggactggat
      901 cagcactatg tcgcccacaa aatcgctcat gccgtagcgg gctgtgcggc tgcagcggcg
      961 aataagggca aatgtcagga cggcgcgatc ggtgcggatg tgggtgagat tgtcggggag
     1021 gctttggtta aaaataccga ttttagcgat atgaccccgg aacaattaga tctggaagtt
     1081 aagaaaatta ccgcctatgc caaacttgcg gcaggtacag ttgcaggcgt aacgggagga
     1141 gatgtcaata ctgctgcaca aaccgcacaa aacgcggtag aaaataatgc ggttaaagct
     1201 gttgtaactg ctgcaaaagt ggtttataag gtagccagaa aaggattaaa aaacgggaaa
     1261 atcaacgtta gagatttaaa acagacgttg aaagacgaag gttataattt agccgacaac
     1321 ctgaccacct tattcgacga aacattggat tggaacgatg ccaaagccgt tattgatatt
     1381 gtcgtcggaa cagagctgaa tcgcgctaat aaaggggaag cggcacaaaa ggtcaaggaa
     1441 gttttagaaa aaaatcgtcc ttatatccct aataaaggtg ctgtaccgaa tatgagtaca
     1501 tacatgaaaa ataatccttt tggaaaacag ctggctcaaa tttcagaaaa gacaacgctt
     1561 ccgacgcagc aagggcagtc tgtcttcttg gtaaaaagaa accaagggtt attaaaaacc
     1621 ggtgataggt tttatttaga tggccaacat aaaaatcatt tagaggtttt tgataaaaat
     1681 gggaacttta agtttgttct aaatatggat ggttcgctta accaaatgaa aactggggca
     1741 gcaaaaggtc gtaaattaaa cttaaaatag gaaattttat ggaaacattg aatgatataa
     1801 aaaaaatctt gattaatgtg gggctttatc aagggtttga tttgacagat ccaaaagtat
     1861 cagaagaagt taatcatgaa acagctaata tgaaatggat taaagattat acttcagacg
     1921 ggaattggga taatgaattt aaggaggatt taaaaaactt tttagattat atggaagtat
     1981 gccaattagc cctaaacgat aaaaatttca aaattgccag taattcttta tttatggcta
     2041 tgatttacgc aggtaatcta tctcttatat ttgattcaat aaaaactgat atatcaacat
     2101 tattgagtgc tgagtataaa aagaatagtt tttcatggcc atctcttgat gaatagaaag
     2161 caagttgtag cctgcatgaa atctaaaacc catgcataag gtgtgggctt cagtatacgc
     2221 gttccatgat ttacggccat atgccgtctg aaaagctcaa ttttttcaga cggcatttgt
     2281 tatgaaagta aatatttaga ttccctgtat actgtttaga ctcgtgtgtg ctgagtaagc
     2341 tgtagtctgc atgaaaccta aaactcgctc aaaattaagc taagacatta gcagggcaag
     2401 ggcgaaaatt gaatcttaaa taaggtgatt cagatgaaaa attttaatgt agtaaaagaa
     2461 agtttaagag agttaggaat taaacaagga tttgatcttt atgagaaagc cacaactgaa
     2521 aaattgaata gtgaagatcc tcttgactta caatggcttt ctaactattc atctgattgg
     2581 aatgatgaat tagaagaaga ctttgattct ttttttcagc atatgaagga atatcaatat
     2641 gctattgaca atgaagacat taaatctgca tgtagttcac tatgtgaagc tatgctctat
     2701 gttggtaata ttaaaaattt ttttgagttt ctcaaaagcg atatgattag actgttgaga
     2761 ggtgaaagta aaacaacaga ctttcaatgg ccgcaatttg atgaatagca gcaagctgta
     2821 gcctgcatga aacctaaaat ccatgcgtaa ggtgtgtgct tcagcacgca cgcgttccat
     2881 gatttacggc tcaatgccgt ctgaaaagct cacaattttt cagacggcat ttgttatgca
     2941 agtaaatatt cagattccct atatactgcc cagatgcgtg ctgaagacac cccctacgct
     3001 tgctatttga aacagctcca agtcaccaaa gacgtcaact ggaaccaggt acaactggcg
     3061 tacgacaaat gggactataa acaggaaggc ttaaccggag ccggagcagc gattatcgca
     3121 ctggccgtta ccgtggtcac ctcaggcgca ggaaccggag ccgtattggg attaaacggt
     3181 gcggccgccg ccgcaaccga tgcagcattt gcctctttgg ccagccaggc ttccgtatcg
     3241 ttcatcaaca acaaaggcga tgtcggcaaa accctgaaag agctgggcag aagcagcacg
     3301 gtgaaaaatc tggtggttgc cgccgctacc gcaggcgtag ccgacaaaat cggcgcttcg
     3361 gcattgaaca atgtcagcga taagcagtgg atcaacaacc tgaccgtcaa cctggccaat
     3421 gcgggcagtg ccgcactgat taataccgct gtcaacggcg gcagcctgaa agacaatctg
     3481 gaagcgaata tccttgcggc tttggtgaat actgcgcatg gagaggcagc cagtaaaatc
     3541 aaacagttgg atcagcacta cattacccac aagatagccc atgccatagc gggctgtgcg
     3601 gcagcggcgg cgaataaggg caagtgtcag gatggtgcga taggcgctgc agtcggtgag
     3661 attgttggtg aggctttggt taagaatacc gatttcagcg gtatgactgc ttctgaaatt
     3721 gaaaaagcta aagcgaatat tactgcgtat
//
LOCUS       DAXIEB010000143         3742 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000143 DAXIEB010000000
VERSION     DAXIEB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.143"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            332..3581
                     /gene="nalP"
                     /locus_tag="ACFOA3_001906"
                     /pseudo
     CDS             332..3581
                     /gene="nalP"
                     /locus_tag="ACFOA3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
BASE COUNT         1012 a         1014 c         1001 g          715 t
ORIGIN      
        1 gttaaagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttcatc
       61 cactataact agggaaattc aaattaagtt agaattatcc ctatgagaaa aagccgtcta
      121 agccggtata aacaaaataa actcattgag ctatttgtcg aaagttcaaa tttccatttt
      181 aaaacaatta gtaaaatcga gtttatccta gttgtccaag acaaccccta taataatata
      241 attcaaaata taaaaatggg ttacatctaa acattacgga atttttattc cctcgcctga
      301 attctattgt cagattcaag gagacctcat catgcgaacg accccaacct tccctacaaa
      361 aactttcaaa ccggctgcca tggcgttagc tgttgcaaca acactttctg cctgcttagg
      421 cggcggcggc ggcggcactt ctgcgcccga cttcaatgca ggcggcaccg gtatcggcag
      481 caacagcaga gcaacaacag cgaaatcagc agcagtatct tacgccggta tcaagaacga
      541 aatgtgcaaa gacagaagca tgctctgtgc cggtcgggat gacgttgcgg ttacagacag
      601 ggatgccaaa atcaatgccc ccccccccga atctgcatac cggagacttt acaaacccaa
      661 atgacgcata caagaatttg atcaacctca aacctgcaat tgaagcaggc tatacaggac
      721 gcggggtaga ggtaggtatc gtcgacacag gcgaatccgt cggcagcata tcctttcccg
      781 aactgtatgg cagaaaagaa cacggctata acgaaaatta caaaaactat acggcgtata
      841 tgcggaagga agcgcctgaa gacggaggcg gtaaagacat tgaagcttct ttcgacgatg
      901 aggccgttat agagactgaa gcaaagccga cggatatccg ccacgtaaaa gaaatcggac
      961 acatcgattt ggtctcccat attattggcg ggcgttccgt ggacggcaga cctgcaggcg
     1021 gtattgcgcc cgatgcgacg ctacacataa tgaatacgca tgatggaacc aagaacgaaa
     1081 tgatggttgc agccatccgc aatgcatggg tcaagctggg cgaacgtggc gtgcgcatcg
     1141 tcaataacag ttttggaaca acatcgaggg caggcactgc cgaccttttc caaatagcca
     1201 attcggagga gcagtaccgc caagcgttgc tcgactattc cggcggtgat aaaacagacg
     1261 agggtatccg cctgatgcaa cagagcgatt acggcaacct gtcctaccac atccgtaata
     1321 aaaacatgct tttcatcttt tcgacaggca atgacgcaca agctcagccc aacacatatg
     1381 ccctattgcc attttatgaa aaagacgctc aaaaaggcat tatcacagtc gcaggcgtag
     1441 accgcagtgg agaaaagttc aaacgggaaa tgtatggaga accgggtaca gaaccgcttg
     1501 agtatggctc caaccattgc ggaattactg ccatgtggtg cctgtcggca ccctatgaag
     1561 caagcgtccg tttcacccgt acaaacccga ttcaaattgc cggaacatcc ttttccgcac
     1621 ccatcgtaac cggcacggcg gctctgctgc tgcagaaata cccgtggatg agcaacgaca
     1681 acctgcgtac cacgctgctg acaacggctc aggacatcgg tgcagtcggc gtggacagca
     1741 agttcggctg gggactgctg gatgcgggta aggccatgaa cggacccgcg tcctttccgt
     1801 tcggcgactt taccgccgat acgaaaggta catccgatat tgcctactcc ttccgtaacg
     1861 acatttcagg cacgggcggc ctgatcaaaa aaggcggcag ccaactgcaa ctgcacggca
     1921 acaacaccta tacgggcaaa accattatcg aaggcggttc gctggtgttg tacggcaaca
     1981 acaaatcgga tatgcgcgtc gaaaccaaag gtgcgctgat ttataacggg gcggcatccg
     2041 gcggtagcct gaacagcgac ggcattgtct atctggcaga taccgaccga tctggcgcaa
     2101 acgaaaccgt gcacatcaaa ggcgatctgc agctgggcgg cgaaggtacg ctgtacacac
     2161 gtttgggcaa actgctgaaa gtggacggta cggcgatgac cggcggcaag ctgtacatgt
     2221 cggcacgcgg caaaggggca ggctatctca accgtaccgg acaacgtgtt cccttcctga
     2281 gtgccgccaa aatcgggcgg gattattctt tcttcacaaa catcgaaacc gacggcggtc
     2341 tgctggcttc cctcgacagc gtcgaaaaaa cagcgggcag tgaaggcgac acgctgtcct
     2401 attatgtccg tcgcggcaat gcggcacgga ctgcttcggc agcggcacat tccgcgcccg
     2461 ccggtctgaa acacgccgta gaacagggcg gcagcaatct agaaaacctg atggtcgaac
     2521 tggatgcctc cgaatcatcc gcaacacccg agacggttga aactgcagcc gccgaccgca
     2581 cagatatgcc gggcatccgc ccctacggcg caactttccg cgcagcggca gccgtacagc
     2641 atgcgaatgc cgccgacggt gtacgcatct tcaacagtct cgccgctacc gtctatgccg
     2701 acagtaccgc cgcccatgcc gatatgcagg gacgccggct gaaagccgta tcggacgggt
     2761 tggaccacaa cgctacgggt ctgcgcgtca tcgcgcaaac ccaacaggac ggtggaacgt
     2821 gggaacaggg cggtgttgaa ggcaaaatgc gcggcagtac ccaaaccgtc ggcattgccg
     2881 cgaaaaccgg cgaaaatacg acagcagccg ccacactggg catgggacgc agcacatgga
     2941 gcgaaaacag tgcaaatgca aaaaccgaca gcattagtct gtttgcaggc atacggcacg
     3001 atgcgggcga tatcggctat ctcaaaggcc tgttctccta cggacgctac aaaaacagca
     3061 tcagccgcag caccggtgcg gacgaacatg cggaaggcag cgtcaacggc acgctgatgc
     3121 agctgggcgc actgggcggt gtcaacgttc cgtttgccgc aacgggagat ttgacggtcg
     3181 aaggcggtct gcgctacgac ctgctcaaac aggatgcatt cgccgaaaaa ggcagtgctt
     3241 tgggctggag cggcaacagc ctcactgaag gcacactggt cggactcgcg ggtctgaagc
     3301 tgtcgcaacc cttgagcgat aaagccgtcc tgtttgcaac ggcgggcgtg gaacgcgacc
     3361 tgaacggacg cgactacacg gtaacgggcg gctttaccgg cgcgactgca gcaaccggca
     3421 agacgggggc acgcaatatg ccgcacaccc gcctggttgc cggtctgggc gcggatgtcg
     3481 aattcggcaa cggctggaac ggcttggcac gttacagcta cgccggttcc aaacagtacg
     3541 gcaaccacag cggacgagtc ggcgtaggct accggttctg acggacagga agcagacagc
     3601 cgcaaagatc acggtctttg cggctgtttc ttatgaaaag aaaaccctat tccaattgcc
     3661 tgcttctatt gtttcaagac ttcttccaaa gattcggcat taatcagatg tatagaggat
     3721 taacaaaaat caggacaagg cg
//
LOCUS       DAXIEB010000144         3579 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000144 DAXIEB010000000
VERSION     DAXIEB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.144"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..335
                     /locus_tag="ACFOA3_001907"
     CDS             <1..335
                     /locus_tag="ACFOA3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6898853.1"
                     /translation="SIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAA
                     GVPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLI
                     HASADNTD"
     gene            349..522
                     /locus_tag="ACFOA3_001908"
     CDS             349..522
                     /locus_tag="ACFOA3_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898854.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            447..779
                     /locus_tag="ACFOA3_001909"
                     /pseudo
     CDS             447..779
                     /locus_tag="ACFOA3_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            776..1186
                     /locus_tag="ACFOA3_001910"
     CDS             776..1186
                     /locus_tag="ACFOA3_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6898855.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKTQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEMRQKERNSSQG"
     gene            1191..1739
                     /locus_tag="ACFOA3_001911"
                     /pseudo
     CDS             1191..1739
                     /locus_tag="ACFOA3_001911"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1755..1940
                     /locus_tag="ACFOA3_001912"
                     /pseudo
     CDS             1755..1940
                     /locus_tag="ACFOA3_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2259..2621)
                     /gene="ssrA"
                     /locus_tag="ACFOA3_001913"
     tmRNA           complement(2259..2621)
                     /gene="ssrA"
                     /locus_tag="ACFOA3_001913"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2684..3289)
                     /locus_tag="ACFOA3_001914"
     CDS             complement(2684..3289)
                     /locus_tag="ACFOA3_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6898856.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT          895 a          816 c          948 g          920 t
ORIGIN      
        1 tcagcattta ccgtaaagcg ttttgggagc gttaccgcgc cgaccaaatg ccggaagcgg
       61 tcgcgttcca attttttgat gcctgcgtca accacggtta cggcaatgcc gcccgtatgc
      121 tgcaacgcgc cgcaggcgta ccggacgacg gcgttatcgg agcagtcagc ctcaaagcca
      181 tcaattccct tcccgaaaac gaccttttat tgcggttcaa cgccgagcgt ctggtctttt
      241 ataccaagct cggtacgttc acctctttcg gcaagggctg ggtacgccgt gtggcgcaaa
      301 acctgattca cgcgtctgca gataacactg attaaaggga gataaaccat gtcaaaaaag
      361 tcactcatcg ccctaatgac cgcagccatg cagcccgatt tcagccacag cgacctaggc
      421 attcgctacg ccatgccgac tcagggatgt tggacgcaag cccaccgcaa gagcggggta
      481 gccgccgcga aacgcgcagc caaaaaaaag cgtcacaaat aaccgccttt ttccgatggc
      541 tgggcggctt ggtctctaat ccggccacag gaaaaatcag ccataccaaa ctatgggcaa
      601 acgtcgccgc agccgccatg acttggaagt tcgtgcaggc ggcggacgcg cccgaatggc
      661 tctagtgggc ttatggcgca ttggtcggcg ggtatgcatt aatcaaacgc ggcatcgcgg
      721 cgattccgca gttggcagaa atcaaaaaat ccgcaaatca ggaagggggg cggcaatgat
      781 tgaatttgtc cgagccaaaa aacggctgct ttgggcattt gtgcttttgc ttgtgtggac
      841 gtgcggttac cgatacgccg ccgacaaggc cgaagcggaa caaaccgccc tgattgccac
      901 ctatcggcat tcttctatgg ttgcggcgga acaatatgcc ttgcagctta aaaaaacgca
      961 ggacgaaagg cagcggtggt acgacttttc ccaaaaacaa ggaagaaagc ccgtgaaaaa
     1021 acagtatccg ccgcaaacga aaaaagccgg ctatctgaaa accaaggaag aactgcttgc
     1081 ggaattggct tgccttaaag cggaaatggt tgccctaaaa aagcccgatg ccttaatcca
     1141 tgggaaagaa atgcggcaga aagaacgcaa ctcgtcgcag ggttaaggca atgccatccg
     1201 ttgaactgct gttggagatt gtcggactgc cacgcggcac cttttattac caattggccg
     1261 tccaatcggc agaagacaaa tatgccgatt tgaaacagca tatccatgat atttatcgac
     1321 gacataaggg aagatacggc taccggagga ttgcagcagc catccttcat gcaggaacac
     1381 cggtcaatca caagaaagtc agccgtctga tggcgaagac ggggctgaag gcagtgatac
     1441 ggcggcgcaa ataccgctcg ttcaaaggag aagtcggcaa aattgcgccg aatatcctgc
     1501 gacgctgttt ccatgcagaa aagccgaatg agaaatgggt aacggacgtt accgagttca
     1561 atgtaggcgg agaaaagata tacctttctc cgattatgga tttgtttaac ggggaaatcg
     1621 tcagttaccg tattcaaacc cgcccgactt tcgatttggc cgacgagata ctgaaaggtg
     1681 cgccggagaa accgggatcg tctgaaaagc cgatactgca ttcggatcaa ggttggcaat
     1741 atcagatgtt tttttatagt ggattaaatt taaatcagga caaggcgatg aagccgcaga
     1801 cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat
     1861 acaacaaaga tagaatcaag ttgaaattaa aagggctgag ccttgttcag tacagaattc
     1921 agtccctgaa agccgcttga ttaaactgtc cgactttttg gggtcagttc ggcttcggca
     1981 tttttttatc cgtttggggg taacttgttt ggaaagctgc aagcttataa ataaaggatt
     2041 acatttaagt tttgggtaac ctttttaaaa aaatgcgtga tgacttttgc atttttaagg
     2101 cgttttttgg ggtaattcgt gaaaagttac cccaaaagtt accccataaa tggcgaaaac
     2161 tcaagcatac gccagcatat tcaaggcaac aaaaaagcct tgaaactgtt gaagttcaag
     2221 gcttttttgt gttgcaggat gctgctgaaa atagggtatg gtggaggcgg ggggaatcga
     2281 acccccgtcc gaaagtcctc tacaaagcgt tctacatact tagttgtgtc tatttgaaaa
     2341 tcttatttcc atcatgccga ccaacaggcc ttttggaaac cagttacctt aaatctcatt
     2401 tcctgccaag taacccggta ggaaaccagt caatgtaaga tgacgttgcg gtggctttcg
     2461 ccacacagcc cattgaccga ctgctgcaac ggctagcctt aagcggctaa agcgtaagtt
     2521 tcgtcgtttg cgactatttg aattcagtgt tttacgggaa tctgagaccc cggtatgccc
     2581 gcatctgctt cgcaaccctc gtcgaaacca aggtcgcccc cagaaatggt ttgcaaatta
     2641 tacggatatt gtgcggtgct gccaagtctg tcggagaaat ttgtcagtct tgctgcctta
     2701 atttgcgttt gagcaggatg cggacgcagc cgtcgttgcc ttcccggggt tcggcgtagg
     2761 cgagtacgtc ggggtgttgc atcagccagt ttcgggtcat atttttcaga acgggtttgt
     2821 agcctttgga acctaatccg ctgccgtgga tgatttcgcc gcatacgccg cgtttttggg
     2881 tgaatgcgat gaattcgttg aggacttttt gggcttcttc ctgtgtgtag ccgtgcaggt
     2941 cgacatcggt aacgacggga tagtatccgt ttttcaggcg ttggatgtcg ttttttccct
     3001 gtccgttttt gctgaagctg gcgggcgggt cgttgtatgt gctgccgatg tagaagtagt
     3061 tttcttcgtc ggcgcggttg tctttgggac ggactttgat gggggttttg tcgggcggcg
     3121 cataatattg ctgccggttt tttaatgggg agacttgtcc gactgcttgt gaaaaatcga
     3181 aatcctgttc ttgtttttgc ttgttttttt cggctgcctg tttttctgcc gcttcttttt
     3241 gggcttgttt gcccagttgt ttgaggatgt tttggaagtc ggtattcata ttttttcctg
     3301 ttatttgtcc gatggctgtt tcgggcgggg ttttaatttg ccggaatgtt taccaatcgg
     3361 gggaggatga ttttgttgcc tgcgtatgtt ttttgaaagt gtgattgtat atcaaaaaga
     3421 aatgcggcaa ccgtcggcag tgttgattgc cggaaatgcg gaccggtcga accgatatgc
     3481 ccgaacgcct gataaagttt taaaaacctg ccttgcgaag caggctgacg tgttttgcca
     3541 atcttgaatt gccggaaacg cgaaacacgg aaatctgat
//
LOCUS       DAXIEB010000145         3553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000145 DAXIEB010000000
VERSION     DAXIEB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.145"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFOA3_001915"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFOA3_001915"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6898857.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="ACFOA3_001916"
     CDS             complement(1846..2133)
                     /locus_tag="ACFOA3_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC6898858.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2235..2468
                     /locus_tag="ACFOA3_001917"
     CDS             2235..2468
                     /locus_tag="ACFOA3_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898859.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFOA3_001918"
     CDS             complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFOA3_001918"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6898860.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAILVKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
BASE COUNT          864 a          858 c          893 g          938 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgccccta ccctgcctca
     2341 ccctgttgcc gaacggaatc atccgcattg ttcaaaagat gccttacaac cgccgcacgt
     2401 cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa tacctgcctg aaacgctgtt
     2461 ttttctaaag catcacgacc ttcacgaaaa atgccgtctg aagaatcttt cagacggcat
     2521 ttcctccttg aggcaataac cggattacgg ttaggcggtt ccttgtctga catatcagac
     2581 aactgccgac accctaaaac tcatgccaag aaatccaaac ctatgtccaa cgagcggctg
     2641 ctgtgggtca gatagccgag ggcgatgccg tccacgccgg tttgcgccac gcgcttcagg
     2701 cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg tgtgggggtg ggcggtttgc
     2761 gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt ccatgttgtc cagcaaaatc
     2821 cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca acgtgtccac ttcgatttct
     2881 acacaagtca acggtccgac cgcctgtttt gcctgccgca cggcttgggc gatgctgccg
     2941 caataggcga ggtggttgtc tttgacaagg atggcatcgt ccaaacccat gcggtggttc
     3001 acgccgccgc ccgccctgac ggcgtatttt tgcaggacgc gcagcagggg gatggttttg
     3061 cggctgcaca cgatgtctgt accgtatttg gcgacttcgg caacggcacg cgcggtggcg
     3121 gtggcgatgc cgcttaagtg cgtgaggtag ttgagcgcgg tgcgttcggc ggcgagcagc
     3181 gcgcaggcgt tgccttcgac ggcggcaagc gtctgacctg cgcggacgac ttgcccgtct
     3241 cggatttcgg cttggaagcg gacggacgga tccatcgtct gaaaggcgag gcgcgccaag
     3301 tccatgccgg cgataacgcc gtcttcgcgg ctgacaagga agagtttggc ggttttgtcg
     3361 ggggcgatga cggcggcgga cgtaatatcg ccgcgcctgc ccaaatcttc gctcaaggct
     3421 tgttcgacta tggggcgcaa cagggtgtcg ggtagggaaa agagggtgtt ttcagacggc
     3481 atggtgtttc ctttgttgaa aaagattgat attgcccgat ggggcaaaac ttcattttat
     3541 agtggattaa ctt
//
LOCUS       DAXIEB010000146         3539 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000146 DAXIEB010000000
VERSION     DAXIEB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.146"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(100..573)
                     /gene="nuoE"
                     /locus_tag="ACFOA3_001919"
     CDS             complement(100..573)
                     /gene="nuoE"
                     /locus_tag="ACFOA3_001919"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6898861.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYNLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVILVNNHSMCGFMTEEAIEKKLAELE"
     gene            complement(573..1829)
                     /gene="nuoD"
                     /locus_tag="ACFOA3_001920"
     CDS             complement(573..1829)
                     /gene="nuoD"
                     /locus_tag="ACFOA3_001920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6898862.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCVDWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(1819..2412)
                     /locus_tag="ACFOA3_001921"
     CDS             complement(1819..2412)
                     /locus_tag="ACFOA3_001921"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6898863.1"
                     /translation="MASIQNLYETVIGVLGDQASKVISALGEITVECLPEHYVSIMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(2425..2907)
                     /locus_tag="ACFOA3_001922"
     CDS             complement(2425..2907)
                     /locus_tag="ACFOA3_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6898864.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(2898..3254)
                     /locus_tag="ACFOA3_001923"
     CDS             complement(2898..3254)
                     /locus_tag="ACFOA3_001923"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6898865.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT          849 a          895 c          944 g          851 t
ORIGIN      
        1 tttcagtagg gcggattctt aaatccgaca ttttgccttt ttacccactc tgtcggatac
       61 aagtatccga cctacattta aatcgtcgtt tcagacgacc tactccaact ccgccagttt
      121 cttctcaatc gcctcttcag tcataaagcc gcacatgctg tggttgttga ccagtatcac
      181 cggcgcgtcg ccgcacgcgc ccatacattc gccttcgaca agggtaaatt tgccgtcggg
      241 cgtggtttcg ccgtagccga taccgagttt ttgtttgagg tattcgccgg tagccatacc
      301 gccgcgcagg gcgcagggca ggttggtgca gacggtcagt ttgtatttgc cgacaggctc
      361 aaggttgtac atattgtaga aagtagcgac ttcgtaggct tgtgcaggtg agatgccgat
      421 atagtctgca acaaaggcga tggtttcggg ggcgagccag cctttttcgg tttgtgcaat
      481 acgcaatgcg cccataatgg cagagcggcg ttggtcggca ggatattttg ccaactcaat
      541 atcaatttgt tttaaggatt ctgcggataa cattatcggt caacctcccc gaatacgatg
      601 tcctgcgtac cgatgatggc aacgacgtcg gcgagcatgt ggccttttgc catttcgtcc
      661 atgccttgca ggtgggcgaa gccgggtgcg cggattttca ggcggtaggg tttgttcgcg
      721 ccgtctgaaa tgatgtaaac gccgaactcg cctttcggat gttcgacagc ggtgtaagtc
      781 tcgccctcgg gaacgtgcat accctcggta aagagtttga aatggtgaat caggtcttcc
      841 atacctgttt tcatttcggt acgtttgggc ggggcgaatt tgtggttggt ggtaatgacc
      901 ggaccgggat tgacgcgcag ccagtcaacg cattgtttga tgatgcgtac ggattggcgc
      961 atttcttcca tgcggcagag gtagcggtcg tagcagtcgc cgttcacacc gacgggaatg
     1021 tcgaaatcca ttttgtcgta cacttcgtaa ggctgtgtct tacgcacatc ccattccacg
     1081 ccggaaccgc gcaacatcac gccggtaaag cctttttgca tggcacgctc gggagtgaca
     1141 acgccgatgc cgacggtacg ctgtttccaa atacggttgt cggtcaggag ggtttcgagt
     1201 gtatcgatgt tttttgggaa gcgttcgcag aaggcgtcga taaagtcgag catagtgcct
     1261 tcacgggatt cgttgagctg cttcaatact ttggcgttgc ggaatttgct gctctcgtat
     1321 ttgggcatga agtcgggcag gtcgcggtaa acgccgccgg gacggaagta ggctgcatgc
     1381 atacgtgcgc cggacacggc ttcgtacaag tccatcagct cttcgcggtc gcggaaggcg
     1441 tagaggatgg cggtcatcgc gccgatgtcg aatgcgtgcg aaccgatgcc catcaggtga
     1501 ttgaggatgc gcgttacttc ggcgaacatc acgcgaatat actgggcgcg gataggtaca
     1561 tcgataccgg caagtttttc tactgccaaa caatacgcct gctcattgac catcatggaa
     1621 acgtagtcca agcgatccat atagggcagg gcttgcagat aggttttggt ttcagccagt
     1681 ttttcagtac cgcggtgcag gaggccgata tgagggtcgg cacggacgat ggtttcgccc
     1741 tccaactcca aaatcatacg caatacgccg tgcgccgcag ggtgttgcgg gccgaagttg
     1801 atggtgtagt ttcttaattt attggccacc gtagttctcc tcacggacga tacgcggcgt
     1861 gatctcgcgc ggctcaatgg taacaggttg gtaaatcacg cgtttttgct cttcgtcgta
     1921 acgcatttcc acatagccgg aaatcgggaa gtctttgcgg aacggatgtc cgacaaagcc
     1981 gtaatcggtc aggatgcggc gcaagtccgg gtggttgttg aacatgatgc cgtacatatc
     2041 gaaggcttcg cgttcgtacc aatccgcgct gttgtaaata tcgactacgg attcaactac
     2101 agggaagtcg tcgtctgaaa cccagacgcg tacgcggatg cgttgattgt ttttaacgga
     2161 aagcagctga ctgacgacgg caaagcgttt gccctgccat gcttcgtttt tgtaagtgct
     2221 gtaatcgaca ccgcacaagt caaccagaag ctcgaaatgc aactcttcat ggtcgcgcag
     2281 cgcggtcatg attgaaacat agtgttcggg cagacactcg acggtaatct cgcccaaagc
     2341 tgaaatgact ttgcttgcct gatcgccaag cacgccgatg acggtctcgt ataagttttg
     2401 aatgcttgcc atatcttcct ccccttactc gtcacgcgcg atggtggaag tgcgcttgat
     2461 tttttgttgg agctgaatca ggccgtaaat cagggcttcc gcagtcggcg gacaacccgg
     2521 cacataaaca tctaccggca cgacgcggtc ggcaccgcgc acaacggaat aagaatagtg
     2581 ataatagccg ccgccgttgg cacatgagcc catagacaat acccagcgcg gctcggcgag
     2641 ctggtcgtac actcggcgca gggcgggcgc cattttattg gtgagcgtac ccgccacaat
     2701 catcaggtcg gcctgacggg gggacggacg gaaaataatg ccgaaacggt caaggtcgta
     2761 acgcgccata cccgcgtgca tcatttccac ggcgcagcag gccaagccga aagtaaccgg
     2821 ccacaacgaa ccggtacgca tatagttcaa caccgtatcc gcgctggtgg tgatgaaacc
     2881 ttttttcaaa acgccttcta ttcccattcc agcgcacctt ttttccattc gtaaacaaag
     2941 cctaccgtca gaacgacgat aaacaccagc atagaccaga agccgtatgc gcccaaatct
     3001 ttgaacacca ccgcccacgg cagcataaac gcaacctcca aatcaaacag gatgaagagg
     3061 atggcgacga ggtaatagcg cacatcaaac ttcatccttg cgttttcaaa ggcttcaaaa
     3121 ccgcattcgt aaggcgcgtc tttttcggcg tagtggcgtt tcgggcctaa aatcgtgccg
     3181 agcaggataa acagcacgcc ggccgcgagg ccgatgagga taaagacaaa gacgggaaag
     3241 taagcggaca acatggttac acccaaatcc gttaacaaaa tttctacaat aatttcgtat
     3301 tttagcgaat ttcaaaaacc attaaaaggt aaatatcggc aaaacgccca aaaaaaccca
     3361 ataaatacaa tcatgttatg ataacgattc ttatttgatt ttataaggtc atataaattt
     3421 ttacaccgta attcgtggta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt
     3481 agctcaaaga gaacgattct ctaaggtgct caagcaccaa gtgaatcggt tccgtacta
//
LOCUS       DAXIEB010000147         3472 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000147 DAXIEB010000000
VERSION     DAXIEB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.147"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            217..1689
                     /gene="pyk"
                     /locus_tag="ACFOA3_001924"
     CDS             217..1689
                     /gene="pyk"
                     /locus_tag="ACFOA3_001924"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6898866.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVKLLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVATGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1999..3399
                     /locus_tag="ACFOA3_001925"
     CDS             1999..3399
                     /locus_tag="ACFOA3_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6898867.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKADILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          893 a         1054 c          883 g          642 t
ORIGIN      
        1 acagccccaa aataaattta gcctaagtca taaaaccgtg taattttgcg ccaaaaaagg
       61 cggtttgcag gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc
      121 cgcgttcatc aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac
      181 cgcaaaacac ccgattgaga ccgaaaggac tttcatatga accagacaag ccgcgatctg
      241 acccgcatca gccacaacac taaaatcgtc gccacccttg ggccgggcag caacaacgtc
      301 aaactgttgg aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc
      361 cacggcacgc ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc
      421 gccggacagg aaatcgccat cattgccgac ctgcaagggc cgaaaatccg cgtgggcaaa
      481 atcgccggcg gcggcattga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc
      541 gaaggcgaag gcacgcgcga ggcggtcggt ttggactacc gcgatctgcc cgacgacgtt
      601 gccacaggcg atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc
      661 gaaggcagca ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaaaggc
      721 atcaacaaac gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac
      781 ctgaaaaccg cgattgccat cggttgcgac tacctcgcca tcagctttgt gaaatccgcc
      841 gaagatttgc acatcgcgcg cgccaaagtc gaagaagaaa tgaagggcag cacggccgtg
      901 cgccccggtt tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc
      961 atcctcgccg gcgacggcat tatggttgcg cgcggcgact tggcggtcga agtcggacac
     1021 gccgccgtcc ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc
     1081 agcattacgg cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg
     1141 gaagtcagcg atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc
     1201 gaaaccgccg tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg
     1261 gctgcggaaa aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa
     1321 gcggtcagca ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac
     1381 gccaaagcca tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac
     1441 aacatcaccc tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg
     1501 atgtaccggg gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta
     1561 aacgaagtcg aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc
     1621 attactagcg gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc
     1681 gtcaaataat ccgccctgag tggaaaatgc cgtctgaagc cgatgcccga ggcttcagac
     1741 ggcatttttt gcggcggcgc ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg
     1801 caacatcgga taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt
     1861 ttttatagtg gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt
     1921 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc
     1981 ctttttacgg aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc
     2041 actgcctttg ccgccgcatc cgcacaagcc tccggctacc acttcggcac acagtcggtc
     2101 aacgcgcaaa gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc
     2161 tacaaccctg ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc
     2221 gtgctgccca gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc
     2281 caaggttcga aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca
     2341 tacaaagtca acgacaatct gaccgtgggc ttgggcgtgt acgtcccctt cggctccgcc
     2401 accgaatacg aaaaagattc tgtgttgcgc cacaacatca acaaactcgg tctgaccagc
     2461 atcgccgtcg aacctgtcgc cgcgtggaaa ctcaacgacc gccattcctt cggcgcaggc
     2521 atcatcgccc aacatacttc cgccgaactg cgcaaatatg ccgactgggg gattaagagt
     2581 aaagcagata tattgacggc aaaaccgccc aaacctaacg gtgtagccga agctgcaaaa
     2641 attcaggccg acggacacgc cgatgtcaaa ggcagcgatt ggggcttcgg ctaccaactg
     2701 gcgtggatgt gggacatcaa cgaccgcgcg cgcgtgggcg tgaactaccg ttccaaagtc
     2761 tcgcacacgc tcaaaggcga tgccgaatgg gcggcagacg gcgcggcggc gaaagcaatg
     2821 tggagtacga tgcttgcagc aaacggctac acggcgaatg aaaaagcccg cgttaaaatc
     2881 gttacgcctg agtctttgtc cgtacacggc atgtacaaag tgtccgataa agccgacctg
     2941 ttcggcgacg taacttggac gcgccacagc cgcttcgata aggcggaact ggtttttgaa
     3001 aaagaaaaaa ccgtcgtcaa aggcaaatcc gaccgcacca ccatcacccc caactggcgc
     3061 aacacctaca aagtcggctt cggcggttct tatcaaatca gcgaaccgct gcaactgcgc
     3121 gccggcatcg cttttgacaa atcgcccgtc cgcaacgccg actaccgcat gaacagcctg
     3181 cccgacggca accgcatctg gttctccgcc ggtatgaaat accatatcgg taaaaaccac
     3241 gtcgtcgatg ccgcctacac ccacatccac atcaacgaca ccagctaccg cacggcgaag
     3301 gcaagcggca acgatgtgga cagcaaaggc gcgtcttccg cacgtttcaa aaaccacgcc
     3361 gacatcatcg gtctgcaata cacctacaaa ttcaaataaa cggcccgccg tttgaatgta
     3421 acaatgccgt ctgaaacaga tttccgtttc agacggcatt gttttaggct cg
//
LOCUS       DAXIEB010000148         3470 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000148 DAXIEB010000000
VERSION     DAXIEB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.148"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(231..1295)
                     /locus_tag="ACFOA3_001926"
     CDS             complement(231..1295)
                     /locus_tag="ACFOA3_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6898868.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1296..2054)
                     /locus_tag="ACFOA3_001927"
     CDS             complement(1296..2054)
                     /locus_tag="ACFOA3_001927"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6898869.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDYSTDNTAEI
                     AESLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2129..2204)
                     /locus_tag="ACFOA3_001928"
     tRNA            complement(2129..2204)
                     /locus_tag="ACFOA3_001928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2169..2171),aa:Ile,seq:cat)
     gene            complement(2258..3367)
                     /locus_tag="ACFOA3_001929"
     CDS             complement(2258..3367)
                     /locus_tag="ACFOA3_001929"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6898870.1"
                     /translation="MEAQLGAPIENYAPPKHWTRKQLRSIERVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVRMMPHTAAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLWDGQNDPDLP
                     AINLTGSGSRWETEKRIAASSSFAFGGSNCVLLIG"
     gene            complement(3371..3470)
                     /locus_tag="ACFOA3_001930"
                     /pseudo
     CDS             complement(3371..3470)
                     /locus_tag="ACFOA3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581578.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          776 a          846 c          924 g          924 t
ORIGIN      
        1 gtactggttt ttgttagtct actatacatt gtaacaaagc acttgatgcg gcagcacaag
       61 ccggggaaga cgctgcaaaa ctttgccaat aggctgcgta atatcggggg atattgggat
      121 tttcttattt ttaaggatgg attcggggtg tttctttcca atgaacgaat ataatttggc
      181 ggtttgttgc atcaaaggct ttcagacggc atatgccgtc tgaaagcagt ttatattgca
      241 tccaataatt tgtcggcggt atggttgatg gtaaaacggt tggcgacgca ttgatgtcct
      301 gccgttgcca gtgcgttttt cgtttcaggg ttcaagacga ggcgttcgat ttcgtccgcc
      361 aaagattcag gtgtcagcct gtccagcaga atccccgatt gatgatgttc gataatttcc
      421 ttttgcgccc ccaaagtatt ggaaatcacc gccgttcggc agtacatcgc ctcgcataat
      481 gacaaaccga atgcctccgg gacgaggctg ggcaaaacca cgacatcgct ttggcggtaa
      541 aacgaagcaa gtttttcggt aaacccttca aaagaaacaa atggttctgc tcctgaagca
      601 gatacgtccc gcttcaggcg gcacatataa tccggatgtc cgtcccctgc caatttgagc
      661 ctgatttgag gatatttccg atgcagtatc acacaggctt caatcaggtt ttccaatcct
      721 ttttctggac tgatcctgcc ggcgtaggcg acggtaaaaa aacggctgtc gggtttttct
      781 tgagagggag ggaaacggcc ggtatcgata ccgttatgaa caatccggta tttttcttta
      841 aagggattgt cggcggtttg cacatcgtaa accagacggg aaacgcaaat aaagcggtcg
      901 gtgtttttct gtatcaggcg gtgataaaaa tcggttttgt tggcgacgac attatgtttg
      961 acaaatatca ggcgcactcc ggtcagtttt ttcaaaagta tggataaggc ggcaattttg
     1021 ccggtgtgta tcatcaggtg ggaaatgcgg tttttccgga taaagcgggt aagtgcgtag
     1081 agcgaaaaca gcccgttgag cgtgtgaagg cgtgtcgtga aaacggaaga aacttcggaa
     1141 aagcgtctgt gcatcaactc attattttta ttgacggcgg taaacatcgt gcagccccgc
     1201 agtcccaatg cttttgaaac atcatagaca tattgttcgc ccccgcccca aatcttggcc
     1261 gatacgatat ttaatatcct gaattctttt tccatttaaa attttccgcc ggatttgtac
     1321 agataatata gtttgacata tttaatcatc gtgtaatagc tgtggttgac ggacataatc
     1381 caacccattt tcccatcgag aaaccctttg ttcaggatat aaattttgaa aaaaccccaa
     1441 atcggacgga ggataatgtc cctaacgaaa cgcacgggct ttccctgctc tcggtatttc
     1501 tcggctgaga tggaagtgta tttgttgaat ttgttgaaat attgttccca gttgtcgtat
     1561 gtgtaatggt acataaaatg cttcagacgg cgtttggggt agggggtttg tacggtttcg
     1621 tgtactttgc cttgcaccga actgtctttt ttcggcatca aacggcatac gctgtcggga
     1681 cgcatcgcgc cgtgtgtggc gggatggttg gggaaaaggt tgcggcgttc gacaaaatag
     1741 gcggcataat cgccggtttg gacaattttt gagatttcat cagatagttc cggcgtgcag
     1801 cgttcgtctg catcaatcag gaaaacccat tccccgcctg cctgttcgat ggcaaatgtt
     1861 ttttgcgctc cgaaatcccc gttcaaatgc cgtctgaaga cttttgcgcc caaactctcg
     1921 gcaatttcgg cagtattgtc ggtgctgtag tcgtcgataa cgatgacttc tttatcgaaa
     1981 cggcaacttt caatacattc ccgaatgtgg tttgcttcat ttctagcaac aatcaatacg
     2041 ctgacttttt tcatcatcaa ggtttctcaa agcattttgg tttcacaggg cggcaaaaaa
     2101 gcccaaacat acgtccgggc ttgggaattg gtgggtccgg tggggttcga acccacgacc
     2161 aagggattat gagtcccctg ctctaacccc tgagctacag acccgtcaag agtaggcgcg
     2221 tattgtatag catagcggtg ggattgacaa acttatttca tccgatgagt aaaacgcagt
     2281 tgcttccccc aaaggcaaac gacgagctgg cggcaatgcg tttttcggtt tcccagcggc
     2341 tgccgctgcc ggtcaggttg atggcgggca ggtcggggtc gttctgcccg tcccaaagct
     2401 ggggcggaag tttcccttcg ggattgcttt gccggtcggc aatgccccac gcgaacgcgg
     2461 cttcgattgc gcccgccgcg cccagcgtgt gtccggtttg cggcttggtg gacgtgcagg
     2521 gcgtattgtt gccgaaaacc gctgcaacgg cgcggctttc catactgtcg ttgtggtgcg
     2581 tcccggtgcc gtgcaggtta atccagccga tgtcttcggg cgcaagacct gcgtgttgca
     2641 atgccgtctg aaaggcgagg attgcgcctt gagcgtcggg gcgcggcgtg gaaatatggt
     2701 aggcatcgct gttggctccg tagccgacga gttcggcgta aattatcgca ccgcgccgtt
     2761 tggcgtgttc caattcttcc agcacgaaaa tccccgcgcc ttcgccgatg accagcccgt
     2821 cgcggttcgc gtcgtatggg cgcggggttt tttccggttc gccgttgcgg cggctggcgg
     2881 cataaagcga gtcgaaaaca tacacttcgg acgggaaaaa ttcttcgcct ccgcccgcca
     2941 gcatcatatc ggtcagaccg tatttgatgg cttcgtaggc ataacctatg ccttggctgc
     3001 cggacgaaca cgcgctcgat gtcgggatga tgcgtccttt cagcccgaaa aagatgccga
     3061 tattggcggc ggcggtgtgc ggcatcatac gcacataggt gttggcactg aagttgcgcg
     3121 acatgccggt cagcaacaat tcgcctacat cgccgatgtc tttggtgctg ccgctggaag
     3181 agccgcaggc aacgcccatc cgtccgtcgg taatgctttc gtccccgagc aaaccggcat
     3241 ccgtcaaagc ctgctccgcc gcatcgacgc acaaatacga cacgcgctct atacttctga
     3301 gctgtttgcg cgtccaatgt ttcggcggcg cgtaattttc aatcggcgca cccagttgcg
     3361 cttccaattc ggggaaacgt tcgcgccaat ccactatatt ttcgcaagac gcgttttgcc
     3421 tgattccaaa aattctcaat gccgttaatg tggttctgac ggtctgcaaa
//
LOCUS       DAXIEB010000149         3191 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000149 DAXIEB010000000
VERSION     DAXIEB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.149"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..296)
                     /locus_tag="ACFOA3_001931"
     CDS             complement(<1..296)
                     /locus_tag="ACFOA3_001931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898871.1"
                     /translation="MIASNAKNRAQNPSAAYRPALFGQTLILPPSRTLVQTGTGNTAR
                     AFPIFTGYPSSGLCSSTISASRFSGGSRRKYSRLNLNQYSVASPCRTDLNLIHY"
     gene            351..974
                     /locus_tag="ACFOA3_001932"
     CDS             351..974
                     /locus_tag="ACFOA3_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6898872.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGTLLLSPLAQAGAQREETLADDV
                     ASVMRSSVGSINPPRLVFDNPKEGERWLSDMSARLARFVPDEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1085..2467
                     /locus_tag="ACFOA3_001933"
     CDS             1085..2467
                     /locus_tag="ACFOA3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6898873.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2471..2995
                     /gene="ssb"
                     /locus_tag="ACFOA3_001934"
     CDS             2471..2995
                     /gene="ssb"
                     /locus_tag="ACFOA3_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6898874.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          663 a          856 c          947 g          725 t
ORIGIN      
        1 atatagtgga ttaaatttaa atcagtacgg caaggcgagg caacgctgta ctggtttaaa
       61 tttaatctac tatatttccg cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg
      121 ctgcataatc cggacgacgg ataaccggtg aaaatgggaa atgcccgggc ggtgttgcct
      181 gtgcctgttt ggacaagcgt tcttgagggc ggtaaaatca aggtttgccc gaaaagggca
      241 gggcgatatg ccgcgcttgg attttgtgcg cggtttttcg cgttggaagc aatcatgtct
      301 gtttgaatat ttaatgccgt ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac
      361 cgaccgatac cctacccgtt aatctgcaac gccgccgcct gttgtgtgct gccggcacgc
      421 tgttgctcag cccgctggca caagccggcg cgcaacgtga agaaacgctt gccgacgatg
      481 tggcttccgt gatgaggagt tccgtcggca gcataaatcc gccgaggctg gtgtttgaca
      541 atccgaaaga gggcgaacgt tggttgtccg acatgtcggc acgtttggca aggtttgtcc
      601 ccgatgagga ggagcggcgc aggctgctgg tcaatatcca gtatgaaagc agccgggccg
      661 gtttggatac gcagattgtg ttgggactga ttgaggtgga aagcgcgttc cgccagtatg
      721 caatcagcgg cgtcggtgcg cgcggtctga tgcaggttat gccgttttgg aaaaactaca
      781 tcggcaaacc ggcgcacaac ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca
      841 tcctgcgcca ttaccggaat cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta
      901 acggcagctt gggcagcaat aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct
      961 ggcagtggcg ttgattttga acccgcgccg caaccgaaat acggcgaatc ctgtataatc
     1021 cgaaaatctg ttcactggaa gttcagacgg cattgcaact gttgatgccg tctgaaaaaa
     1081 tatgatggca agagacaacc gcatccaaat gtttccgcac gaatggcgcg ccagtacgac
     1141 gctttccggc gtgtacgcgc tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc
     1201 ggtgtatgcc gcctcgctgc ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat
     1261 gggcatttac gggctgacac aggctctgct gcaactgcct ttgggcatcg cttccgacaa
     1321 gttcgggcgc aagaaaacca tttatgtcgg gctgattgtg tttgcggcgg gcagctttct
     1381 tgccgccgcc gccgatacgc tgcccatgct ggtcgccgca cgcgccatac agggtgcagg
     1441 ggcggtcagt gcggcggtta ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac
     1501 ccgcgcaatg gcgatgatcg gtttgagtat cggtttgacg ttttcggtca gcctcgtcgt
     1561 tgcccccgtg attgccgacg cggtcggcgt tcgcggactg tttatgctga ccggcattct
     1621 gaccgtcatc agcatcggcg tggtggcgtg gatgactccc gatcccgaag tttccaagct
     1681 gcacgaagat acgcaggcgc agccttcgcg cataggcgaa gttttgaaaa accgtaggct
     1741 gctgacgctc gatttcggca ttttcgccct gcacgccgcg caaatggcat tgtttaccgc
     1801 gctgcctttc gcgatgaccc agctcggttt ggaaaaaatc cagcactgga aagtctatct
     1861 gccctcgacc attacgggat tggtggtaat ggttccgctg attatcgtcg gtgagacgcg
     1921 caacaagctc aaacaggttt ttatcttggg tatcgcctgt attgcggcgg cgcagctcgg
     1981 tttgctgtcc ggtatgcgct cggtaggctt gattaccgct tatttggttg tttactttat
     2041 cggttttaat gtgttggaag cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga
     2101 cctgaagggt acggcgatgg gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg
     2161 cggcgcggca ggcggtttgc tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg
     2221 cagcatattg atgctgctgt ggctggtgat tgccgtttta tcgcctgcgc ccaagcccgt
     2281 caaaaacctc agttaccctg tcggcggcgt gtggcaggtc aatcaggaag ggttataccg
     2341 cgccttgtcg gagcttgagg gcgtggaaga catcggtttc agtttcgacg ggcagaccgt
     2401 ctatctcaaa gtgttgcaga agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg
     2461 agtttaaaaa atgtcattga acaaagtcat ccttatcggc cgcctcggac gcgatcccga
     2521 agtgcgctat atgcccaacg gcgaggcggt gtgtaatttc agcgtcgcca ccagcgaaac
     2581 atggaacgac cgcaacggcc aacgtgtaga gcgcaccgag tggcacaaca tcacgatgta
     2641 ccgcaaactg gcggaaattg ccgggcaata cctcaaaaaa ggcgggctgg tttatttgga
     2701 aggcagaatc caaagccgca aataccaagg taaagacggc atcgagcgca ccgcttacga
     2761 tattgtcgcc aacgaaatga aaatgttggg cgggcgcaat gaaaacagcg gcggcgcacc
     2821 ttacgaggaa ggttacggtc agagtcagga ggcttaccaa cgccccgcgc agcaaagccg
     2881 gcagcctgcc cccgacgcgc cgtcccatcc tcaagaagca ccagccgcgc cgcgccgcca
     2941 acccgtgccg gccgccgccc cggtcgagga tattgacgac gacatcccgt tctgaatttt
     3001 acggcagcac atcctcgcgg agggaaatca taaaggacgg agaaacctta aaccttacgg
     3061 ggcagggttt ctcttttttg catttgtgcc ggcttgttgc tgccccctgc gttgggcgga
     3121 acgttgcgat tgcgccgttc tggcgttttg aggcagtgat gcctgccgca cgtccccgct
     3181 gttttcaggc g
//
LOCUS       DAXIEB010000150         3174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000150 DAXIEB010000000
VERSION     DAXIEB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.150"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            113..2881
                     /gene="tdfH"
                     /locus_tag="ACFOA3_001935"
     CDS             113..2881
                     /gene="tdfH"
                     /locus_tag="ACFOA3_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6898875.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRTGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQTGGLKFNSDSGKWER
                     DLQRQFWKTRYYKQYEDPQKLRKYIEGQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LITMSYKF"
     gene            2911..3153
                     /locus_tag="ACFOA3_001936"
     CDS             2911..3153
                     /locus_tag="ACFOA3_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898876.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFYSHAGKP
                     VFDFGYFWLFRVIYESSFPRRRESRHSMLRQFIGND"
BASE COUNT          901 a          786 c          765 g          722 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta ccttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcacgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 ccccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 aacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agcggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atctggaacg gcgcaagcag cgatattttg tacaaacagg
      901 cgggttgaaa ttcaattccg acagcggaaa gtgggaacgc gatttgcaaa ggcaattctg
      961 gaaaaccagg tactataaac aatacgaaga cccccagaaa ctgagaaaat acatcgaagg
     1021 tcaagataaa gactggcggg aaaacctggc gccgcaatac gacattaccc ccatcgatcc
     1081 gtccagcctg aagcagcagt cggcaggcaa tctgtttaaa ttggagtacg acggcgtatt
     1141 caataaatac acggcgcaat ttcgcgattt aaacaccaaa atcggcagcc gcaaaatcat
     1201 caaccgcaat tatcaattca attacggttt atctttaaac tcatatgcca acctcaatct
     1261 gaccgcagcc tacaattcgg gcaggcagaa atatccgaaa gggtcgaagt ttacaggctg
     1321 ggggctttta aaagattttg aaacctacaa caacgcaaaa atcctcgacc tcaacaacac
     1381 cgccaccttc cggctgcccc gcgaaaccga gttgcaaacc actttgggct tcaattattt
     1441 ccacaacgaa tacggcaaaa accgctttcc tgaagaattg gggctgtttt tcgacggtcc
     1501 ggatcaggac aacgggcttt attcctattt ggggcggttt aagggcgata aagggctgct
     1561 gccccaaaaa tcaaccattg tccaaccggc cggcagccaa tatttcaaca cgttctactt
     1621 cgatgccgcg ctcaaaaaag acatttaccg cttaaactac agcaccaata ccgtcggcta
     1681 ccgtttcggc ggcgaatata cgggctatta cggctcggat gacgaattta agcgggcatt
     1741 cggagaaaac tcgccgacat acaagaaaca ttgcaaccag agctgcggaa tttatgaacc
     1801 cgtattgaaa aaatacggca aaaagcgcgc caacaaccat tcggtcagca ttagtgcgga
     1861 cttcggcgat tatttcatgc cgttcgccag ctattcgcgc acacaccgta tgcccaacat
     1921 ccaagaaatg tatttttccc aaatcggcga ctccggcgtt cacaccgcct taaaaccaga
     1981 gcgcgcaaac acttggcaat ttggcttcaa tacctataaa aaaggattgt taaaacaaga
     2041 tgatatatta ggattaaaac tggtcggcta ccgcagccgc atcgacaact acatccacaa
     2101 cgtttacggg aaatggtggg atttgaacgg gaatattccg agctgggtca gcagcaccgg
     2161 gcttgcctac accatccaac accgcaattt caaagacaaa gtgcacaaac acggttttga
     2221 gttggagctg aattacgatt atgggcgttt tttcaccaac ctttcttacg cctatcaaaa
     2281 aagcacgcaa ccgaccaact tcagcgatgc gagcgaatcg cccaacaatg cgtccaaaga
     2341 agaccaactc aaacaaggtt atgggttgag cagggtttcc gccctgccgc gagattacgg
     2401 acgtttggaa gtcggtacgc gctggttggg caacaaactg actttgggcg gcgcgatgcg
     2461 ctatttcggc aagagcatcc gcgcgacggc tgaagaacgc tatatcgacg gcaccaatgg
     2521 gggaaatacc agcaatttcc ggcaactggg caagcgttcc atcaaacaaa ccgaaaccct
     2581 tgcccgccag cctttgattt ttgattttta cgccgcttac gagccgaaga aaaaccttat
     2641 tttccgcgcc gaagtcaaaa atctgttcga caggcgttat atcgatccgc tcgatgcggg
     2701 caatgatgcg gcaacgcagc gttattacag ctcgttcgac ccgaaagaca aggacgaaga
     2761 agtaacgtgt aatgctgata aaacgttgtg caacggcaaa tacggcggca caagcaaaag
     2821 cgtattgacc aattttgcac gcggacgcac ctttttgata acgatgagct acaagtttta
     2881 aaggcagccc gcgttttgtg gaaaaccgca atgccgtctg aaagcccttc agacggcatt
     2941 tgtttcccca aacgcatcat cctgccgcaa gcctatgcca atccgtttta tcgcatcggc
     3001 aactcaaaga aaaatccatt ttattcccac gcagggaagc cggtttttga tttcggttat
     3061 ttttggttgt ttcgggtaat ttatgagtcg tcattcccgc gcaggcggga atctagacat
     3121 tcaatgctaa ggcaatttat cgggaatgac tgaaactcaa aaaactggat tccc
//
LOCUS       DAXIEB010000151         3147 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000151 DAXIEB010000000
VERSION     DAXIEB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.151"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFOA3_001937"
     CDS             complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFOA3_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6898877.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2117..2967
                     /locus_tag="ACFOA3_001938"
                     /pseudo
     CDS             2117..2967
                     /locus_tag="ACFOA3_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT          696 a          817 c          884 g          750 t
ORIGIN      
        1 ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac ggcggaaaag
       61 tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc ccactgaaac
      121 ggcgggggcg ggatggggaa atgccgtctg aaacggtcaa acgacgtttc agacggcatt
      181 tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc aatttccatc
      241 cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt cgcgcgggcg
      301 gcttgtgcgt cttcgacagt caatccgagg gcttttgcca cgccttcacc gtatgccgga
      361 tcgcaacggt agcagttgcg gatatggcgg tatttgatga agtcgggcgc gtcgcccatt
      421 gcggcggcgg tgttgccgaa caatgcctgt ttctgcgcgt cgttcatcag gttgaacagg
      481 gcgcgcggtt ggctgaagta gtcgtcatca tcttggcggt agtcccagtg tgccgcgtcg
      541 ccgttgattt tcaaaggcgg ttcagcgaag tcgggctgct gctgccattg tccgaagctg
      601 ttgggttcgt agtgcggcag gctgccgtag ttgccgtcgg ctctgccttg tccgtcgcgc
      661 tggttgctgt gaacagggca acgcggacgg ttgacaggga tttggcggaa gttcacgccc
      721 aagcggtagc gttgcgcgtc ggcgtaattg aacaaacgcg cctgcaacat tttatctggg
      781 ctggcgccga caccgggaac gaggttgctc ggtgcgaagg cggattgttc cacatcggcg
      841 aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag cggatagtct
      901 tttttcggcc aaactttggt caggtcaaac ggatgataag gtactttttc ggcatcggct
      961 tcgggcatca cttggatgta catcgtccat ttcggaaact cgccgcgttc gatggcttcg
     1021 tataagtcgc gctgatggct ttcgcggtcg tcggcgatga ttttggcggc ttcttcgttg
     1081 gtcaggtttt taatgccttg ttgggtgcgg aaatggaatt tcacccaaaa acgctcgcct
     1141 gcttcgttcc agaagctgta ggtatgcgaa ccgaagccgt gcatatggcg gtagccggcg
     1201 gggatgccgc ggtcgctcat cacgatggta acttggtgca gtgcttcggg cagcagcgtc
     1261 cagaagtccc agttgtttgt ggcagagcgc atattggtgc gcgggtcgcg tttgacggct
     1321 ttgttcaggt cggggaactt acgcgggtcg cgcaggaaga acacgggcgt gttgttgccg
     1381 accacatccc agttgccttc ttcggtatag aacttcaacg caaaaccgcg gatgtcgcgt
     1441 tctgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa catctcggtt
     1501 tttttgccga cttcgctgaa gattttggcg cgggtgtatt tggtgatgtc gtgcgttacg
     1561 gtaaacgtac cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg gatgacttcg
     1621 cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag agggccgcga
     1681 ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt catggtcaga
     1741 tgggttacag ggcatttgga ggtagtcatc gctcttgttc cttttctcag gttggtcaaa
     1801 tgggggtaaa cggcttacag tacgatttgg cggaaagcgt attcgtaacc ggtttcttga
     1861 ttgcaataaa tttcttgaat cgacatttta tttccctttt gtaaaaacta tggatgcgac
     1921 tatacgccaa gattttcgct attaaaacta tgaaatcgat ttaatattat tataagcaat
     1981 cggttcttga ttttcgtttg ttttttgtta tcgaacggaa tccgaacccg ctcattaaaa
     2041 ccatttataa tgcaatgacg ctttgcggca ttttttgcgc cgacaggctg aaaataacaa
     2101 ttttccccac attatcatga ccttactcgg atataagctc aaacagaccc agcagctcaa
     2161 ccagcggctg caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac ttgaacgcga
     2221 ggtcgaaaac tggctgtcgg acaatcccct gctcgaacgc aaagacacgg atgaattttc
     2281 cgatgccggg tttcccatta caccgcgccc gcgcgtcaaa tcggcggaga cgaaggcgaa
     2341 gatatgctgt ccaacatcgt cggcgaagaa gatttcaagc aatacctgca cgcgcaagtg
     2401 tgcgaacacc cccttcccga ccaagaagcc gcctgtgtcc acatccttat cgatttcctt
     2461 gacgagcagg gttatctgac cgacagcatc gaagacatcc tcgaccatac gcccttagag
     2521 tggatgttgg atgaagcaat gctgcaacac gcgctgaccg cattgaaaaa attcgacccg
     2581 gcaggcgtgg ccgccgccga tttgaacgaa tcgctgatac tgcagataga aagattgggc
     2641 gaatgtgctg ccaaacccgc cgccctgcat atcgtccgaa acgccctcga cagcattgac
     2701 ggcaaccgca gccaaaccct cgcacgaata aaaaaacacc tgccccaaac cgacagcggc
     2761 acactcgaag ccgcactcga cctcattgct tcgctcaatc cctttcccgc cgccggtttt
     2821 gcctcgtcca cacccacgcc gtattctgac gaggcgctcg ccaacctgct ggctttccgc
     2881 ggcatggagg tttctcgccg caccattgcc aaatacagag aatcctttga gattccggca
     2941 gcacacaaac gcaaaaccgc agaataattg ccgaataatc ttataaagac aacaaaccaa
     3001 aagccggcat ttctgcgaaa gcgggaatgc cgaatccgtc cgcgcggaaa cctgcatccc
     3061 gtcattcccg cgaaagaggg aatctagaaa cgcaaagttg caagaattta tcggaaatga
     3121 ccgaaactca acgaacctgg attcccg
//
LOCUS       DAXIEB010000152         3131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000152 DAXIEB010000000
VERSION     DAXIEB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.152"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..1986
                     /locus_tag="ACFOA3_001939"
     CDS             <1..1986
                     /locus_tag="ACFOA3_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898878.1"
                     /translation="IQSVQDSETYQSKQQNASAQVTVGYGFSAGGDYSQSKINADYAS
                     VNEQSGIFAGENGYQINVQNHTDLKGGLITSTQKSEDDGKNTFSTGTLTFEDLANHAE
                     YKGEAIGLGVSGSVAGDNPNQNSRIYTVNQSGMSSTMGYGKDSDKQYATTHSGINTAN
                     ITIRDNEKQQQITGISAEEAKRAVKADVTTDNYAEHSGSLKNNFDKDKVQSEIDLQVK
                     VTQEFDSNRKALRAEINKAADEKRQKAEDIRRENYIDGKNGYNTAESLALDKSADKLE
                     KVAFYTDLALGGLYGIGDSTALTYVTGSAVANPAIRAGTRPRQFWFEKCDGDSLYCSN
                     RNYDDTADRKIYDKNGQEIDTKDPNYTGMVEIGDKRQISDLNEIEPNKVTGSIVTTND
                     GILNPRNDALKNGIKQQKWEVDKEGILLIYNPPTGNSISELLYAAYDKTNDLLGGRLP
                     LTSAQKANLELNQYAESKGYKIDSNNHSRGGLTRSVSDKYSVRHNLPLPYNNVQFFGS
                     AANVDDYTNYMNEYGRKDFNISATTHYTDFVGRTPLVALRSKHIVGGNEPTGGVEDKW
                     FLYSHSSYFAEKPTPYLVNEHGFYMDKNGDLIKDNSGNPIKMTEENKLEYSTKVPNPY
                     LDDFEQKWIKGQTHNKVSGDNPSLPVPVRTKKQQGER"
     gene            1986..2417
                     /locus_tag="ACFOA3_001940"
     CDS             1986..2417
                     /locus_tag="ACFOA3_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898879.1"
                     /translation="MKKYFFSVCLLLTGCGVLFDDPLTRFWNGGYFLFPDKEIASDGI
                     YKTSDTDLHYYESLSLEQRKRYLKFKPIKDKCEHKYSDRTKYDYATQMANIVRCFNEQ
                     GYQSETAFILNGHTITQKGSICTKDEPKQCDNVMRSYIVLF"
     gene            2459..2710
                     /locus_tag="ACFOA3_001941"
     CDS             2459..2710
                     /locus_tag="ACFOA3_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898880.1"
                     /translation="MKPIYFIIFSFLLTACVVNNDDYFAEPPTPYLINEHGFYMNKKG
                     DLIRDSHGNAIKMTEENKHNFPKESANPYYEKWIKQKRK"
     gene            2919..>3131
                     /locus_tag="ACFOA3_001942"
     CDS             2919..>3131
                     /locus_tag="ACFOA3_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898881.1"
                     /translation="MKYLISGCLKNKMMWCASNRVGNKLPTLRLLDRIAGIYITGTEK
                     GVLAAQAGKDINIIAGQISNQSEQGQT"
BASE COUNT         1148 a          553 c          629 g          801 t
ORIGIN      
        1 attcaaagcg tacaagatag cgaaacctat caaagcaaac aacaaaacgc cagtgcacaa
       61 gttaccgtag gttatggctt cagtgccggt ggcgattaca gccaaagcaa aatcaacgcc
      121 gattatgcgt ctgtgaatga acaaagtggc atatttgcgg gtgaaaacgg ttatcaaatc
      181 aatgtgcaga accacaccga cttgaaaggc ggtttgatta cttccaccca aaaatcggaa
      241 gacgatggca aaaacacatt ctcaacgggt acgctcacct ttgaagacct agcgaatcac
      301 gccgaatata agggtgaagc gattggtttg ggtgtttcag gcagcgttgc gggcgataat
      361 ccaaatcaaa acagccgtat ttataccgtt aatcaaagcg gtatgagtag cacaatgggc
      421 tatggcaaag acagcgacaa acaatacgcc acaacccatt caggcattaa cactgccaat
      481 atcaccattc gtgataacga aaaacaacag caaatcacag gaatatcggc ggaagaagcg
      541 aaacgggctg ttaaagccga tgtaacaacc gacaactacg ccgaacattc aggcagcctg
      601 aaaaacaatt tcgataaaga caaagtgcaa tcagagattg atttgcaggt taaggttaca
      661 caggagtttg atagtaatcg caaagccttg cgtgcagaaa tcaacaaagc agcagatgaa
      721 aaaagacaaa aagccgaaga tataagaaga gaaaattata ttgacggcaa aaacggttat
      781 aacaccgcag aatctttggc tttggataag tctgccgaca aattggaaaa agtcgctttt
      841 tacactgatt tggcattagg tggattgtat ggaattggcg atagcacggc tttaacttat
      901 gtaacaggta gtgcagtggc taatccagcc attcgagcgg gtacaagacc aagacagttt
      961 tggtttgaga aatgtgacgg cgatagtttg tattgttcta atagaaatta tgacgatacg
     1021 gcggatagaa aaatttatga taaaaacggg caagaaattg acactaaaga tccaaattac
     1081 actggaatgg ttgagattgg tgataaacgg caaatctctg atttaaatga gattgaacct
     1141 aacaaagtaa ctggaagcat cgttactaca aatgacggta tattgaaccc acgaaatgac
     1201 gctctcaaaa atgggattaa gcaacaaaaa tgggaagtag ataaggaagg tattttgctt
     1261 atctacaatc cgccaacagg caattctatt tctgaacttt tgtatgccgc ttatgataaa
     1321 actaacgatt tgttaggcgg tcgtttgcct ttaacttctg cacaaaaagc gaaccttgaa
     1381 ttaaatcaat atgcagaaag taaaggttat aagattgata gtaacaatca tagtcgtggc
     1441 ggtttaactc gaagtgtttc tgataaatat tcagtcagac ataatctgcc tttaccgtat
     1501 aacaatgttc aattcttcgg tagtgcggca aatgtagatg attacacaaa ctacatgaac
     1561 gaatatggta ggaaagattt taatatttct gccacaacac attacacaga ttttgttggt
     1621 agaactccat tagttgcatt gcgtagtaag catattgttg gtggaaatga accaacaggc
     1681 ggagttgaag ataaatggtt cttatattcg cacagcagtt attttgcaga aaaaccaacc
     1741 ccataccttg taaatgaaca cggtttttat atggataaaa atggcgacct gataaaagat
     1801 aattcaggaa atcctataaa aatgacggaa gaaaataaat tggaatattc aacaaaagtt
     1861 ccaaatccat atttagatga ttttgaacag aaatggataa aaggacaaac tcataacaag
     1921 gtgtcaggtg ataatccgtc tcttccagtt ccagttagaa caaaaaaaca acagggagag
     1981 cgataatgaa aaaatacttc ttttcagtct gcttgttatt aacaggctgt ggggttttgt
     2041 ttgatgaccc actaacaaga ttttggaatg gcggttattt tttgtttcca gacaaagaaa
     2101 tagcaagtga tggaatttat aaaacttcgg atactgactt gcattattac gaaagtttgt
     2161 cgcttgaaca aagaaaacgc tatctaaaat ttaagcctat taaggataaa tgcgaacata
     2221 agtattcaga tagaacaaaa tatgattacg ctacacaaat ggcaaatatc gttaggtgtt
     2281 ttaatgaaca aggctatcaa tctgaaactg cttttatatt gaatggacat acaataactc
     2341 aaaaaggtag tatttgcaca aaagatgaac ccaaacaatg cgataatgtt atgcgtagtt
     2401 atatcgtttt attttaattg aaatgcaact tgaaaattga caatacagga gcaacaaaat
     2461 gaaaccgatt tattttatta ttttttcttt tttattaact gcttgcgtgg ttaataatga
     2521 tgattatttt gctgaaccgc ctacaccata cttaattaac gaacacggtt tttatatgaa
     2581 taaaaaaggt gatttaatta gagatagtca cggtaacgca attaaaatga cagaagaaaa
     2641 taaacacaat ttccccaaag aatccgctaa tccttattac gaaaaatgga taaaacaaaa
     2701 acgaaaataa ataacaaagc aaacatagtg taggcaactt gttgcccacg cgttaatgat
     2761 aactttcagg cagcctgaaa gcctaaaacc acaaggaaag ctccaaaaat gaacaaccgc
     2821 ttacacaaag tcatcttcaa ccgcaacaca gcaagcgtag ggtgggcaac ttgttgccca
     2881 cgcagttcaa tcagatacac ataaaatgat gttttaatgt gaaatatttg atttcaggct
     2941 gtctgaaaaa taaaatgatg tggtgtgcat caaaccgcgt gggcaacaag ttgcccaccc
     3001 tacgtttgct agaccgaata gcaggtattt acatcacagg cacagaaaaa ggtgttttag
     3061 cagcacaagc tggtaaggac atcaacatca tcgccggtca aatcagcaat caatcagagc
     3121 aagggcaaac c
//
LOCUS       DAXIEB010000153         3096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000153 DAXIEB010000000
VERSION     DAXIEB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.153"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>3096
                     /locus_tag="ACFOA3_001943"
     CDS             <1..>3096
                     /locus_tag="ACFOA3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fhaB protein"
                     /protein_id="HFC6898882.1"
                     /translation="SSGNVAIDANGRLVNSGTMAAANVKDTDNTAEHKVNIRSQGVEN
                     SGTAVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLN
                     NQGKLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASSHSSTTT
                     PTTATGTGTATVSISNITAPTFADGIIRTHGALDNSGSIIANGQTGVSAQQGLNNAGQ
                     IDIHQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETGQLDNAHGK
                     LLSAEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSDRDVAIQAK
                     SLSNNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKA
                     NNLDNAAQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAWQVNNIGTGRIYGDNIAI
                     AATRLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATG
                     KAQRIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLRE
                     GTQHELGWFVYNNESDHLRTPDGVAHENWHKYDYEKVTQETQVTGTAPAKIIAGSDLI
                     IDSKAVFNSDSRIIAGGNLLVQTEKDGLHNEQTFGEKKVFSENGKLHNYWREKHKGRD
                     STGHSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNS
                     FTPLPSSSLYIINPANKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGY
                     YEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQ
                     LTSDIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLK
                     NSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSAEQTLLLNAGNN
                     INSQSTAASSQNTQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSEQG
                     QT"
BASE COUNT         1027 a          709 c          687 g          673 t
ORIGIN      
        1 tcttccggta atgtggcgat tgatgcaaat ggccgtttag tcaatagtgg cacgatggct
       61 gccgccaatg tgaaagatac ggataataca gcggaacaca aagtcaatat ccgcagtcag
      121 ggcgttgaaa acagcggtac ggcggtatcg caacaaggca ctcaaattca cagtcagtcg
      181 attcaaaaca ctggcacatt attgtcctca ggcgaaatat tgattcacaa ttcgggcagc
      241 ctgaaaaatg aaacatcagg caccattgaa gccgctcgtt tggctattga taccgacaca
      301 cttaataatc aaggcaaact ctctcaaaca ggttcacaaa aactccatat tgatgcacaa
      361 ggcaaaatgg ataaccgtgg ccgcatgggt ttacaagata ccgcaccaac cgcgtcaaat
      421 ggttcaagca atcaaaccgg caatagttac aatgcatctt cccattcatc cactaccaca
      481 ccaacaacgg caacaggtac gggtactgca accgtttcta tatcaaacat aactgcgcct
      541 acctttgctg atgggataat tcgcactcat ggtgcactgg ataattcagg cagtattatt
      601 gccaatggtc aaacaggtgt tagtgcgcaa caaggtttaa ataatgcagg acaaatagac
      661 attcatcagt taaatgcaaa aggttcggcg tttgacaatc acaatggaac aattatcagt
      721 gatgcggtcc acattcaagc cggcagcctg aataatcaaa acggcaacat cacaacacgc
      781 caacagttag agattgaaac cggtcaactg gataacgctc atggcaagtt attatcagca
      841 gaaatagcgg atttagccgt ttcaggcagc ctgaacaatc aaaatggcga aatagcgacc
      901 aatcaacaac tgattattca cgatggtcag caatctaccg ctgtcattga taatacgaat
      961 ggcacgatac aatcagaccg tgatgttgct attcaggcaa aatcgttatc caacaacggc
     1021 acacttgccg ctgataataa actggatatt gcgttacaag atgattttta tgtagaacgc
     1081 aatatcgtgg cgggcaatga attgtcgctc agtacacgag gcagcctgaa aaattcacat
     1141 accttgcaag caggaaaacg cattcggatt aaagcaaata accttgataa tgcagcacaa
     1201 ggcaacattc aatccggcgg tacgacagac attggcacgc agcacaattt aaccaataga
     1261 ggcttgattg acggacaaca aaccaaaatc caagcctggc aagtgaataa tatcggtaca
     1321 ggtcggattt atggtgacaa tatcgctatt gcggctaccc gcttagacaa tcaagatgaa
     1381 aacggtacag gtgccgccat tgcggcacgc gaaaacctga atttaggcat tggacaatta
     1441 aataaccgtg aaaacagtct gatttacagc ggtaacgata tggcggttgg cggcgcatta
     1501 gataccaatg gccaagccac aggcaaagcc caaaggatac acaatgccgg cgcaaccatt
     1561 gaagctgcag gcaaaatgcg tttaggtgta gaaaagctgc acaataccaa tgagcatttg
     1621 aaaacgcagt tggtagaaac agggcgcgag catattgttg attacgaagc atttggacga
     1681 cacgaattat tgcgagaagg cacgcaacat gaattaggct ggtttgtcta taacaatgaa
     1741 tcagaccact tacgcacccc tgatggagtg gcgcatgaaa attggcataa atacgattat
     1801 gaaaaagtaa cgcaagaaac tcaagtaacc ggaactgcgc ctgctaaaat cattgcaggt
     1861 agcgatttga ttattgatag caaagcagtc ttcaacagcg acagccgaat catcgccggc
     1921 ggcaatttgc ttgtgcaaac agaaaaagac ggtttgcata acgagcaaac ctttggcgag
     1981 aagaaagtct tcagcgaaaa tggcaagttg cacaactatt ggcgtgagaa acataaagga
     2041 cgagactcaa cgggacatag cgaacaaaat tacactttgc cggaggaaat cacacgcaac
     2101 atttcactgg gttcatttgc ctatgaatcg catcgcaaag cattaagcca tcatgcgccc
     2161 agccaaggca ctgagttgcc gcaaagcaac ggtatttcgc taccctatac gtccaattct
     2221 tttaccccat tacccagcag cagcttatac attatcaatc ctgccaataa aggctatctt
     2281 gttgaaaccg atccacgctt tgccaactac cgtcaatggt tgggtagtga ctatatgctg
     2341 gacagcctca aactagaccc aaacaattta cataaacgtt tgggtgatgg ttattacgag
     2401 caacgtttaa tcaatgaaca gatcgcagag ctgacagggc atcgtcgttt agacggttat
     2461 caaaacgacg aagaacaatt taaagcctta atggataatg gcgcgactgc ggcacgctcc
     2521 atgaatctca gcgttggcat tgcattaagt gccgagcaag tagcgcaact gaccagcgat
     2581 attgtttggt tggtacaaaa agaagttaaa cttcctgatg gcggcacaca aaccgtattg
     2641 gtgccacagg tttatgtacg cgttaaaaat ggcgacatag acggtaaagg tgcattgttg
     2701 tcaggcagca atacacaaat caatgtttca ggcagcctga aaaactcagg cacgattgca
     2761 gggcgcaatg cgcttattat caataccgat acgctagaca atatcggtgg gcgtattcat
     2821 gcgcaaaaat cagcggttac ggccacacaa gacatcaata atattggcgg cattctttct
     2881 gccgaacaga cattattact caacgcaggc aacaacatca acagccaaag caccgccgct
     2941 agcagtcaaa atacacaagg cagcagcacc tacctagacc gaatggcagg tatttatatc
     3001 acaggcaaag aaaaaggtgt tttagcagcg caggcaggca aagacatcaa catcatcgcc
     3061 ggtcaaatca gcaatcaatc agagcaaggg caaacc
//
LOCUS       DAXIEB010000154         2912 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000154 DAXIEB010000000
VERSION     DAXIEB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.154"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..1256
                     /locus_tag="ACFOA3_001944"
     CDS             <1..1256
                     /locus_tag="ACFOA3_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898883.1"
                     /translation="AEKAAIRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGL
                     TTGSLGGILAGGGTSLAAPYLDKAAENLGPAGKAAVNVLGGAAIGYAAGGNVGTAAVG
                     ANVDWNNRQLHPSEYKLAEQYADIVASRLNISKEEALGRIIRHMQRTVDYATAKADNF
                     RTDDAIISILGANNLPKQDQHYTNPNYNKQYIQNYVRDFNLANKYNYFWKTPTQSRDY
                     KVGVVTDIGKAALNSGIGIFESAANLATGEIDPNSWAYVHMGRIGYTHPEYGNALENT
                     MTLGVAAVTAGRAGTQVRARNTQQTKLVGELTANGIKFNPNNVVAITKTTDGKIVWLE
                     TGNTRAGLNHIIERHGQEFLKKGISESEIPNYLMTAVKNGQIVGMQRTRPIYEFTYNG
                     TKHRVAIDIGNNGFIVGANPKRVVK"
     gene            1253..1624
                     /locus_tag="ACFOA3_001945"
     CDS             1253..1624
                     /locus_tag="ACFOA3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898884.1"
                     /translation="MKKIRLLLEFHCFPVWFYDENDEIIENDWCEELLPQEILSDNHL
                     KELCIWLADEFDKLYCDEMGNFDYEKLQDEFSFQTKENEQEFIDKLTEFRTKFTQKCF
                     GFCALQDNLPKIENGKIVSWQ"
     gene            1869..2270
                     /locus_tag="ACFOA3_001946"
     CDS             1869..2270
                     /locus_tag="ACFOA3_001946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898885.1"
                     /translation="MTLGVAAVTAERTGTQAGKIGTVKPSSFANESLLNDHFIKHGTE
                     FNVKNASEYLKIGQDTVNKGIPVQYDYNGSKRTGYVQFLGTDSKGQSKFIFVGTNENG
                     SITTIHTKSGKDFWRTLNNNPKNKTIYPKTR"
     gene            2272..2661
                     /locus_tag="ACFOA3_001947"
     CDS             2272..2661
                     /locus_tag="ACFOA3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898886.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            2701..>2912
                     /locus_tag="ACFOA3_001948"
     CDS             2701..>2912
                     /locus_tag="ACFOA3_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6898887.1"
                     /translation="MPQAAMLGQGAKNKPQNKHLTSVADKNGASSSVGYGSDQDSQSS
                     ITKSGINTRNIHITDEAGQLARTGRTA"
BASE COUNT         1035 a          507 c          605 g          765 t
ORIGIN      
        1 aagccgaaaa agccgccatc cgcgcatccc tcggccaagt aaacgcctac cttgccgaaa
       61 accaaagccg ctacgacacc tggaaagaag gcggcatagg caggagcata ctgcacggag
      121 cggcaggcgg actgacgacg ggcagcctcg gcggcatact ggccggcggc ggcacttccc
      181 ttgccgcacc gtatttggac aaagcggcgg aaaacctcgg tccggcgggc aaagcggcgg
      241 tcaacgtact gggcggggcg gcaatcggct atgcggcggg cgggaatgtc ggtacggcgg
      301 cagtaggggc gaatgtcgat tggaacaata ggcagctgca tccgagtgaa tataaattag
      361 cagaacaata tgcagatata gtggcttctc gtctaaacat ttcaaaagaa gaagcacttg
      421 ggcgaattat tcggcatatg caacgaaccg tagattacgc aacggctaaa gcggataatt
      481 tccgtactga tgacgccatt atttcaattt taggagcaaa caatttaccc aagcaagatc
      541 aacattacac taatccgaac tacaacaaac aatacattca aaattatgtt cgtgatttca
      601 atttagccaa taaatacaat tatttttgga aaactcctac tcaatcaagg gattataaag
      661 ttggagttgt tacagatatt ggtaaggctg cattaaatag cggtataggt atatttgaat
      721 cggctgcgaa tttagcgaca ggggagatag atccgaactc atgggcttat gtacacatgg
      781 ggcgtatagg ttatacacat cctgaatatg gaaatgctct agaaaataca atgacacttg
      841 gtgtcgctgc agttaccgct gggcgtgctg gaacacaagt aagagcgaga aatacacaac
      901 aaactaaact tgttggagag ttaacagcta atggtatcaa gtttaatccg aataatgtag
      961 ttgctattac taaaacaaca gatggaaaaa ttgtttggtt agaaacagga aatacacgag
     1021 caggactaaa ccatattata gaaaggcacg gacaggaatt tttgaaaaag ggaatatctg
     1081 aatctgaaat tcctaattat ttgatgactg cggttaaaaa tggacaaatt gtaggaatgc
     1141 aacgcacaag acctatatat gaattcacct ataacggcac aaaacaccgt gtggctattg
     1201 atataggcaa taatgggttt attgttggtg ccaaccccaa aagagtagtt aaatgaaaaa
     1261 aataagattg ttacttgaat ttcactgttt tccagtatgg ttttatgatg aaaatgatga
     1321 aattattgaa aacgattggt gtgaagaatt attaccacag gaaattttat cagacaatca
     1381 tctgaaagaa ttgtgcattt ggctagcaga tgaatttgat aaattatatt gtgatgaaat
     1441 gggtaatttc gattatgaaa agttacaaga tgaattttcg tttcaaacaa aggaaaatga
     1501 acaagagttc attgataaat taacagaatt caggacgaaa tttacacaaa aatgttttgg
     1561 tttttgtgcg ttacaagata atttaccaaa aattgaaaac ggtaaaattg tttcttggca
     1621 gtgagcgtaa cgtacgcaac ttgttgccca cgctacacaa ccacaaccct taacctcttt
     1681 tgcaggctgc tcatcaatat ctttcctatt tttgatataa acataatttt gtatcaatga
     1741 cccaatgatt aaaatgatct cacaggaagc caataaatac tgtatattca gtcttttaat
     1801 gcctatccaa gtggactcta aggttaggtt gtacacatcc tgaatatggg aatgctctag
     1861 aaaatacaat gacacttggt gtcgctgcag ttaccgctga gcgtactgga acacaagcag
     1921 gaaaaatagg aacagttaaa ccgagtagtt ttgctaatga aagtttgtta aatgaccatt
     1981 tcattaaaca cggtactgaa tttaatgtaa aaaatgcaag cgaatactta aaaattggtc
     2041 aagatactgt aaataaaggt attcctgttc aatatgatta taatggttca aaacgaactg
     2101 gatatgttca gtttttgggt actgattcca aaggtcaatc taaatttatt tttgttggaa
     2161 ccaatgaaaa tggtagtatt accacgatac atacaaaatc aggaaaagac ttttggagaa
     2221 ctttgaataa caatccaaaa aacaaaacaa tttatccaaa gacaagataa aatgttatat
     2281 aaatgtaaaa tcaaaaagtt agataacacc attgaagagg cagtattgat tgaaataaat
     2341 ggttatgaac tacattgttt tatatcatac tgcccattca aaatatttga aaatcaattt
     2401 tatgaagtgg aattatccta tgaaatcttt aatgattaca tactggataa atcaacttca
     2461 aatattggat ttagaaaaat agatgatagt ttcaagtatg aaataacggc agaattaaaa
     2521 aataatcatc taaccctaca aaatatacaa tttgaagaag atgagtattt aataaactac
     2581 tcttatcttg ataattgttt ggttacatta acggttgata ggataagtgt ttcatttatt
     2641 agagaaattc aggcagcctg aaacctgttg tggataccgt tggcagttta gcgattggtt
     2701 atgccacagg cggcaatgct gggacagggc gcaaaaaata aacctcaaaa caaacacctg
     2761 acaagcgtag ccgataaaaa tggcgcaagt tcatcagtgg gttatggcag cgaccaagac
     2821 agtcaaagca gcatcacgaa aagcggcatc aacacccgca acatacacat caccgacgaa
     2881 gcgggacaac ttgcccgaac aggcaggact gc
//
LOCUS       DAXIEB010000155         2909 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000155 DAXIEB010000000
VERSION     DAXIEB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.155"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            266..589
                     /locus_tag="ACFOA3_001949"
     CDS             266..589
                     /locus_tag="ACFOA3_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6898888.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            703..2880
                     /gene="tdfF"
                     /locus_tag="ACFOA3_001950"
     CDS             703..2880
                     /gene="tdfF"
                     /locus_tag="ACFOA3_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6898889.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTATTDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQGETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          706 a         1031 c          740 g          432 t
ORIGIN      
        1 ggaacaccac aggcacacgc atcgaacggc tgaatcaaag attcagaccg atggcagtcc
       61 gcacccgagt ttatgcggca aacagcgagg ctacggcaac ccgccccctc tccctgtggg
      121 agagggttag ggagagggcg gtaagccgca ggcttacatc aaagccgata acgctttcgt
      181 tacaactccg cccactgaaa gcagcctgca acgaagccaa aacgacaaac cgcatcgtaa
      241 accacccaac ccataggaga accccatgca aaacgaaacc atcaacctga aacagcacct
      301 tgccgccatc aaagaatact ggcagcccga aatcatcaac cgccacgggt tccaattccg
      361 tttggtcaaa cttttgggcg attacggctg gcatacgcac ggatacagcg acaaagtgct
      421 gtttgccgtg gagggcgaca tggcggtgga cttcgccgac ggcggcagca tgacgatacg
      481 cgagggcgag atggcggtcg tgccgaagtc ggtgtcgcac cgcccgcgtt cggaaaacgg
      541 ctgctcggtg gtgctgattg agttgtccga cccgtccgag gccgtctgaa aacgaagttt
      601 ccgaaggaag ctgagtttct gcgaagctaa aagcagcctg caccttcaat caatatgccg
      661 aaaatacaac cccaccgcac accaacacac aaaggaaatc ccatgacacg cttcaaatat
      721 tccctgctgt ttgccgccct gttgcccgtg tacgcgcagg ccgatgtttc tgtttcagac
      781 gaccccaaac cgcaggaaag cactgaattg ccgaccatca ccgttaccgc cgaccgcacc
      841 gcgagttcca acgacggcta cactgtttcc ggcacgcaca ccccgctcgg gctgcccatg
      901 accctgcgcg aaatcccgca gagcgtcagc gtcatcacat cgcaacaaat gcgcgaccaa
      961 aacatcaaag cgctcgaccg cgccctgttg caggcgaccg gcaccagccg ccagatttac
     1021 ggctccgacc gcgcgggcta caactacctg ttcgcgcgcg gcagccgcat cgccaactac
     1081 caaatcaacg gcatccccgt tgccgacgcg ctggccgata cgggcaatgc caacaccgcc
     1141 gcctatgagc gcgtagaagt cgtgcgcggc gtggcggggc tgctggacgg cacgggcgag
     1201 ccttccgcca ccgtcaatct ggtgcgcaaa cgcccgaccc gcaagccatt gtttgaagtc
     1261 cgcgccgaag cgggcaaccg caaacatttc gggctgggcg cggacgtatc gggcagcctg
     1321 aacaccgaag gcacgctgcg cggccgcctg gtttccacct tcggacgcgg cgactcgtgg
     1381 cggcagcgcg aacgcagccg cgatgccgaa ctctacggca ttttggaata cgacatcgca
     1441 ccgcaaaccc gcgtccacgc aggcatggac taccagcagg cgaaagaaac cgccgacgcg
     1501 ccgctcagct acgccgtgta cgacagccaa ggttatgcca ccgccttcgg cccgaaagac
     1561 aaccccgcca caaattgggc gaacagccgc caccgtgcgc tcaacctgtt cgccggcatc
     1621 gaacaccgct tcaaccaaga ctggaaactc aaagccgaat acgactacac ccgcagccgc
     1681 ttccgccagc cctacggcgt agcaggcgtg ctttccatcg accacaacac cgccaccacc
     1741 gacctgattc ccggttattg gcacgccgac ccgcgcaccc acagcgccag cgtgtcattg
     1801 atcggcaaat accgcctgtt cggccgcgaa cacgatttaa tcgcgggtat caacggttac
     1861 aaatacgcca gcaacaaata cggcgaacgc agcatcatcc ccaacgccat tcccaacgcc
     1921 tacgaatttt cccgcacggg tgcctacccg cagcctgcat cgtttgccca aaccatcccg
     1981 caatacggca ccaggcggca aatcggcggc tatctcgcca cccgtttccg cgccgccgac
     2041 aacctttcgc tgatactcgg cggcagatac agccgttacc gcaccggcag ctacgacagc
     2101 cgcacacaag gcatgaccta tgtgtccgcc aaccgtttca ccccctacac aggcatcgtg
     2161 ttcgacctga ccggcaacct gtctctttac ggctcgtaca gcagcctgtt cgtcccgcaa
     2221 tcgcaaaaag acgaacacgg cagctacctg aaacccgtaa ccggcaacaa tctggaagcc
     2281 ggcatcaaag gcgaatggct tgaaggccgt ctgaacgcat ccgccgccgt gtaccgcgcc
     2341 cgtaaaaaca acctcgccac cgcagcagga cgcgacctga gcggcaacac ctactaccgc
     2401 gccgccaacc aagccaaaac ccacggctgg gaaatcgaag tcggcggccg catcacgccc
     2461 gaatggcaga tacaggcagg ttacagccaa agcaaaaccc gcgaccaaga cggcagccgc
     2521 ctgaaccccg acagcgtacc cgaacgcagc ttcaaactct tcactgccta ccactttgcc
     2581 cccgaagccc ccagcggctg gaccatcggc gcaggcgtgc gctggcaggg cgaaacccac
     2641 accgaccctg ccacgctccg catccccaac cccgccgcca aagcccgcgc cgccgacaac
     2701 agccgccaaa aagcctacgc cgtcgccgac atcatggcgc gttaccgctt caatccgcgc
     2761 gccgaactgt cgctgaacgt ggacaatctg ttcaacaaac actaccgcac ccagcccgac
     2821 cgccacagct acggcgcact gcggacagtg aacgcggcgt ttacctatcg gtttaaataa
     2881 gatcgtctga aaacggagtt tctgcgaag
//
LOCUS       DAXIEB010000156         2898 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000156 DAXIEB010000000
VERSION     DAXIEB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.156"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..291)
                     /locus_tag="ACFOA3_001951"
     CDS             complement(<1..291)
                     /locus_tag="ACFOA3_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898890.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELS"
     gene            complement(361..579)
                     /locus_tag="ACFOA3_001952"
     CDS             complement(361..579)
                     /locus_tag="ACFOA3_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898891.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            592..762
                     /locus_tag="ACFOA3_001953"
     CDS             592..762
                     /locus_tag="ACFOA3_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6898892.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(807..1112)
                     /locus_tag="ACFOA3_001954"
     CDS             complement(807..1112)
                     /locus_tag="ACFOA3_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898893.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVSELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1009..1428)
                     /locus_tag="ACFOA3_001955"
     CDS             complement(1009..1428)
                     /locus_tag="ACFOA3_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898894.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYNRALGYGN"
     gene            complement(1400..1636)
                     /locus_tag="ACFOA3_001956"
     CDS             complement(1400..1636)
                     /locus_tag="ACFOA3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898895.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            complement(1639..1803)
                     /locus_tag="ACFOA3_001957"
     CDS             complement(1639..1803)
                     /locus_tag="ACFOA3_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898896.1"
                     /translation="MGQVAFYEKMIELWSSKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(1806..2012)
                     /locus_tag="ACFOA3_001958"
     CDS             complement(1806..2012)
                     /locus_tag="ACFOA3_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898897.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRH"
     gene            complement(2009..2554)
                     /locus_tag="ACFOA3_001959"
     CDS             complement(2009..2554)
                     /locus_tag="ACFOA3_001959"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6898898.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(2710..>2898)
                     /locus_tag="ACFOA3_001960"
     CDS             complement(2710..>2898)
                     /locus_tag="ACFOA3_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HFC6898899.1"
                     /translation="KALIQLRNHRFYQEFVKLRDIDKESGLMAMTKLCPKYGISSRTG
                     YTIINEMSRPAAQQAALF"
BASE COUNT          663 a          789 c          683 g          763 t
ORIGIN      
        1 gctcagttcg tcgaaacggc aaaacaggtt gaaatcgatg cgggtaatga ggctgtgttc
       61 gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga acatggacaa
      121 caggggatag gcgggacggc cgcggtggtc tctaaggtaa cgggtttttt gacggttcag
      181 gtattgttcg atcggctgcc aatcaatcac ctggtccaac ttcaatagtg ggaagcggtc
      241 gatgtgttta gcaatcatgg cttgtgcggt ttgccggaag aaggtgctca tgagaaatct
      301 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tctcggtagg
      361 tcattctttg atacccatta aatattttgt ccgcttgtac aatttcttaa cccacgaaat
      421 atttacaaat gtataaacct ttgctacaac ttcgccgtca tagtgcgcat tttctcgtgc
      481 agcccgaaat ttcgccctgg cttcttcagg gttgtccgcc caaatgctca atgaccagga
      541 cttgccgtta aagcggtaag aaaacgtgta ctcattcata ggagaaacct tatgtatttt
      601 gaaatctata aagacgcaaa aggcgaatac cgttggcgtt tgaaagcagc caaccatgaa
      661 atcatcgctc agggcgaagg ctacaccagc aagcaaaact gtcagcacgc agtcgatttg
      721 ctgaaaagca ctaccgccgc tacccctgta aaagaggtat aaaatccgct ttcaccctca
      781 gcccgcgccc tacgcgggct tttttgtcag tttccgactt tgcgggagtg attgcccgcc
      841 cagtcgcgcc aagcctgatt ttggttctcc agttccgaaa catagccgcc aaactcagcg
      901 gcgtgttcca acagcgttac tgtcttgccg tctttcggcg gattcgggcg taccggcgca
      961 accatcaatg cggcaggcgg tgtcggcatg accgcctttt cgacaacctt aatttccgta
     1021 gccgagggcg cggttgtaga gctgcaagcc gtgatggcca aagccgtcaa tacaaccgcc
     1081 gcctgcattt ttacggtctt gagtaaggac attttcgatt tcctttttat tttccgtttt
     1141 cagacggctg acttccgcct gttttttcgc caaagccatg ccgacagcgt gcgccttgac
     1201 ttcatatttt ttagcttccg cacgcgcctg ttccagctcg cgggcgtagt tttgagccga
     1261 caacagcagg gcttgcgctt tgtctttttc catcttgtca atgaccgcct gctgcttcgc
     1321 aaaagccgac ttgtagcctt gatggtgcga caccgccaag agagtgccga caagcgcgat
     1381 gatggcaatc ggttgccagt tattcgccag cagtttcacg agattcattc tcgacctcct
     1441 gacgtttgac gctgaccaac gaacgcgcca ccgcatagcc gccgacaatg cccaaataca
     1501 ccgcccaaat ctccgccgac gggtcgggca gcatcacaaa cttaaccgtc cccgccgcgc
     1561 aggcaacgtt tgcccacagt ttcgagtgcg acacattgcc tgtcgccggg tttttaaaaa
     1621 tatccaaaat acgcattgct attccacact tttggtttgc aggtgccgtt tcagcatttc
     1681 ctgataattg gccagttcgc cctccgcaaa ttcgaacgca gccaagtctg cctgttcgct
     1741 tgcctcgcgg cttttgctcg accatagctc aatcatcttt tcgtaaaacg caacctgtcc
     1801 catgattaat gacgattctt gcgtttgcgc gccgcacgtt tcgcggctgc cacgcctgag
     1861 tttcttaaat ttgggcgtgg ataggtgtgt atcggacgaa catttggcat ctgtataagt
     1921 gcgcggttta cctgctcatt actaggattt tctccaatca attttgtaag aaaataggaa
     1981 gcagcttctt tcaatttacc aataatattc atactcttgc cgcccccaat tccatcgcaa
     2041 tcgcgtccgc aatcgcgcgg cagatgcccc atttggtcgt cttaaacaag gccaaatcag
     2101 tgtcgttgct gataaaaaac ggctcaaaca caatgccgcc ggcttgcgca taagccaggc
     2161 gcgaatgccg acccgcattg tcgggtttaa agccgtcttc gccgcgcagt ttccagccgg
     2221 ttttcttggc aacggctttg ctcaacacct gacaccagcg tttgttttta acggtactca
     2281 aggcttcgat gcctgtcgcc gctttgctga cggcagcgtt ggtgtgaaac tcaatcgcca
     2341 catccgagcc gcgaatcagt tttacggctt cacgcagcgg catattgcct ttgcctgtgc
     2401 cgtcggtttt aacagtcaaa ccgtagtcat cgcgcaaaat agcagccacg atattgcgca
     2461 tatcctgcgc caagtccgcc tcacggtcgc tgccgttgac cgcacccggg tcggtgttgc
     2521 tgtgtccagc ggttaaggtt acggttttgc ccattcatca tctccaaaaa aggttgattg
     2581 cataacattt gaaaacccca ttaaaccgtc tttaacccat cgtcccgaac ggtagatgtt
     2641 tcatcccctg caaaccaaaa aaaagaccgt ctgaatacag acggccaaag cctggtcaca
     2701 catcactgcc taaaataaag ctgcctgctg tgccgcaggt cggctcattt cattgataat
     2761 cgtatatccc gttcgtgaag agatgccgta tttagggcat agcttcgtca tcgccataag
     2821 cccgctctcc ttatcaatat cgcgcaattt gacaaactcc tgataaaacc tatggtttct
     2881 caactgtatt aacgcctt
//
LOCUS       DAXIEB010000157         2854 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000157 DAXIEB010000000
VERSION     DAXIEB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.157"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            102..1316
                     /gene="gltS"
                     /locus_tag="ACFOA3_001961"
     CDS             102..1316
                     /gene="gltS"
                     /locus_tag="ACFOA3_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6898900.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVKKIKLLRDFNIPEPVAGGL
                     IAAIILFALHEVYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            1415..2431
                     /locus_tag="ACFOA3_001962"
     CDS             1415..2431
                     /locus_tag="ACFOA3_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6898901.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            2442..2639
                     /locus_tag="ACFOA3_001963"
     CDS             2442..2639
                     /locus_tag="ACFOA3_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898902.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            2657..2806
                     /locus_tag="ACFOA3_001964"
     CDS             2657..2806
                     /locus_tag="ACFOA3_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898903.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT          648 a          720 c          825 g          661 t
ORIGIN      
        1 acggcgattc tttgacaagc tttaactatt cccctaatat cttcgcgcct gcgtcccctt
       61 cgggcggaca tgaagaaaaa cattttagga ggtcaaacga tatggaatgg gagtttaaca
      121 gctattacac gctgattgct gctaccctgg tcttgttggt gggtaaattt ttagttaaaa
      181 aaatcaagtt gttgagggat tttaatattc ctgagcctgt ggcaggcggc ctgattgccg
      241 cgattattct gtttgcgctg cacgaagtgt acggcgtgag cttcaaattt gaaaaaccgc
      301 tgcaaaatgc gtttatgctg attttcttca cgtccatcgg cttgagcgcg gatttttccc
      361 gtttgaaggc gggcggtttg ccgctggtgg tttttaccgc gattgtgggc ggatttatct
      421 tggtgcaaaa ctttgtcggg gtcggactgg ctacggcttt gggtttggac ccgctcatcg
      481 gtctgattac cggttcggtg tcgctgacgg gcggacacgg tacgtcaggt gcgtggggac
      541 ctaattttga aacgcaatac ggcttggtcg gcgcaaccgg tttgggtatt gcatcggcta
      601 ctttcgggct ggtgttcggc ggcctgatcg gcgggccggt tgcgcgccgc ctgatcaaca
      661 aaatgggccg caaaccggtt gaaaacaaaa aacaggatca ggacgacaac gcggacgacg
      721 tgttcgagca ggcaaaacgc acccgcctga ttacggcgga atctgccgtt gaaacgcttg
      781 ccatgtttgc cgcgtgtttg gcgtttgccg agattatgga cggcttcgac aaagaatacc
      841 tgttcgacct gcccaaattc gtgtggtgtc tgtttggcgg cgtggtcatc cgcaacatcc
      901 tcactgccgc attcaaggtc aatatgttcg accgcgccat cgatgtgttc ggcaatgctt
      961 cgctttcgct tttcttggca atggcgttgc tgaatttgaa actgtgggag ctgaccggtt
     1021 tggcggggcc tgtaaccgtg attcttgccg tacaaaccgt ggtgatggtt ttgtacgcga
     1081 cttttgttac ctatgtcttt atggggcgcg attatgatgc ggcagtattg gctgccggcc
     1141 attgcggttt cggcttgggt gcaacgccga cggcggtggc aaatatgcag tccgtcacgc
     1201 atactttcgg cgcgtcgcat aaggcgtttt tgattgtgcc tatggtcggc gcgttcttcg
     1261 tcgatttgat taatgccgcg attctcaccg gttttgtgaa tttctttaaa ggctgatttt
     1321 ccgcctttcc gccaaagcac ctgcaaggtt taccgcctgc aggtgctttt gctatgatag
     1381 ccgctatcgg tctgcaccgt ttggaaggaa catcatgtat cggaaactca ttgcgctgcc
     1441 gtttgccctg ctgcttgccg cttgcggcag ggaagaaccg cccaaggcat tggaatgcgc
     1501 caaccccgcc gtgttgcaag gcatacgcgg caatattcag gaaacgctca cgcaggaagc
     1561 gcgttctttc gcgcgcgaag acggcaggca gtttgtcgat gccgacaaaa ttatcgccgc
     1621 cgcctacggt ttggcgtttt ctttggaaca cgcttcggaa acgcaggaag gcgggcgcac
     1681 gttctgtatc gccgatttga acattaccgt gccgtctgaa acgcttgccg atgccaaggc
     1741 aaacagcccc ctgctgtacg gggaaaccgc tttgtcggat attgtgcggc agaagacggg
     1801 cggcaatgtc gagtttaaag acggcgtatt gacggcagcc gtccgcttcc tgcccgtcaa
     1861 agacggtcag acggcatttg tcgacaacac ggtcggtatg gcggcgcaaa cgctgtctgc
     1921 cgcgctgctg ccttacggcg tgaagagcat cgtgatgata gacggcaagg cggtgaaaaa
     1981 agaagacgcg gtcaggattt tgagcggaaa agcccgtgaa gaagaaccgt ccaaacccac
     2041 gcccgaagac attttggaac acaatgccgc cggcggcgat gcgggcgtac cccaagccgc
     2101 agaaggcgcg cccgaaccgg aaatcctgca tcctgacgac ggcgagcgtg ccgataccgt
     2161 taccgtatca cggggcgaag tggaagaggc gcgcgtacaa aaccagcgtg cggaatccga
     2221 aattaccaaa ctttggggag gactcgatac cgacgtgcaa aaagagttgg tcggcgaaca
     2281 acgcaagtgg gcgcaggaaa aaatcagcaa ctgccgacaa gccgccgcgc aggcagaccg
     2341 gcaggaatac gccgaatacc tcaagctgca atgcgacacg cggatgacgc gcgaacggat
     2401 acagtatctt cgcggctatt ccatcgatta ggggcaaacc gatgactacc gtccccaaaa
     2461 gcaggattcc cgtcaaaccg ctgcccgaaa aaaccacaga cgaagccaaa gtcgaaaaat
     2521 ggcggcagct cggtgcggaa cacggtttgt cgggcgaatg ggcagttgcc gccagattgg
     2581 gcgagaacgg ttttaccgaa gaacagatgg aaaatatcgc caacctgttc ggcagataaa
     2641 gggaaaattg acggaaatgc cgtctgaaac cctgttatcg gtttcagacg gcattttgac
     2701 taatacggta cgcaggcgca aaacagccgg ctttttatgt gtttcctatg ctgatgttcc
     2761 aacacacagg acgatacaaa aagcgtcgcc ctatgtgccg tcctgattcg gaaagggtta
     2821 cgctcctccc aaatatagtg gattaacaaa aacc
//
LOCUS       DAXIEB010000158         2831 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000158 DAXIEB010000000
VERSION     DAXIEB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.158"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..238
                     /locus_tag="ACFOA3_001965"
     CDS             <1..238
                     /locus_tag="ACFOA3_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6898904.1"
                     /translation="MRKACRNRPLSETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARA
                     AYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            342..899
                     /locus_tag="ACFOA3_001966"
     CDS             342..899
                     /locus_tag="ACFOA3_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6898905.1"
                     /translation="MGFGCREGREFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMSLFPQALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGKLGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLGAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(986..1954)
                     /gene="ispH"
                     /locus_tag="ACFOA3_001967"
                     /gene_synonym="lytB"
     CDS             complement(986..1954)
                     /gene="ispH"
                     /locus_tag="ACFOA3_001967"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6898906.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1983..2510)
                     /gene="lspA"
                     /locus_tag="ACFOA3_001968"
     CDS             complement(1983..2510)
                     /gene="lspA"
                     /locus_tag="ACFOA3_001968"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6898907.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVTVSAYLVRAILRDEF
                     ATLGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKY"
BASE COUNT          655 a          831 c          708 g          637 t
ORIGIN      
        1 tatgcgcaaa gcctgccgca accgtccgct gtcggaaacg caaaccaaac gcaaccggta
       61 tttatcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc gtaaattccg
      121 ctatgcgcgg gcggcctatt tcggactgat taaagtgagt gcgcaaagcc acctgaaggc
      181 gatgtgtttg aaccttttga aagccgccaa caggctaagt gcgcccgctg ccgcctaaaa
      241 ggcagcccgg atgcctgatt atcgggtatc cggggaggat taagggggcg tttgggtaga
      301 attaggaggt atttggggcg aaaacagccg aaaatctgtg tttgggtttc ggctgtcggg
      361 agggaaggga attttgcaaa ggtctcagcc tgcccgaaat catccgcgac ctgctcgaaa
      421 tgccgtctga aaccgccatt gccgacatca cacgcactta ttccgcacat tacctcaatc
      481 ccaacaaccg caatatgtcc ttatttcctc aggcactgcc ctgtctggac aagctcaaag
      541 cacaaggata ctggcttgcc gtcgccacgg gcaaagggcg ggcgggtttg gacaacgcca
      601 tcagccaaac cgccaccggc ggctattggc tcgccaccgc ctgcgcgggg gaatatccct
      661 ccaaaccctc gcccgaaatg gtattcggaa tctgcggcaa actgggactc gatccgaaag
      721 aggcattggt cgtcggcgat acggcgcacg acctgcatat ggcggcgaac gcgggtgcgg
      781 cggcagtcgg cgttgccacc ggcgcacatt cgcgcgaaca gctccttggc gcaccgcatc
      841 tcgccgtatt ggacgacttg tccgaactgc ccggttttct tgcacagcat tacgcctgat
      901 tggtttccgc atccggcacg cggcaaaaat gccgtctgaa gcctgttcag acggcatttg
      961 tgttgcccaa acattcaacg cttgctcaac gcttgcacaa atcgggtttg gtttcgccct
     1021 cgcggcgcaa ctctttgggc aggacgaaca cgatgctttc ttccgcaccc tcgccttcgc
     1081 gcacggtttc atgcccccac ccgcgtatgg ttgccaatac ttcctgcacc aacacttcgg
     1141 gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc ctcaaaccat gcgcgttgca
     1201 ggtagcctgc attatccacc atatacgcat cgattccgcg cgatgccgcc acttcgcgca
     1261 agcggttgct gttagacgaa ttgggcgaac cgaccacaat cacgatgtcg cactgttctg
     1321 ccaactcttt gacggcggtt tgccggttgg tcgtcgcata gcagatgtct tccttgtgcg
     1381 gattgcggat attggggaaa cgcgcgttca gcgcggcgat gatgtctttg gtttcatcga
     1441 ccgagagcgt ggtttggctg acataggcga gtttgtcggg gtttctgact tcgagttttg
     1501 ccacatctcc taccgtttcg accaaaagca ttttgcccgg cgcaagctgc cccatcgttc
     1561 cttcgacctc gacatgaccc ttatgcccga tcatgatgat ttcacagtct tgggcatcca
     1621 gtcgggcgac ttccttatgc actttcgtca ccagcgggca agtcgcatca aatacccgga
     1681 aaccacgttc cgccgcttct tgctgcaccg ccttcgatac gccgtgtgcc gaataaacca
     1741 gcgtcgcgcc cggcggcact tccgccaagt cttcgataaa caccgcgcct ttttcacgca
     1801 ggttatcgac gacgaatttg ttgtggacga cttcgtggcg cacataaatc ggcgcgccga
     1861 attcttccaa agcacgttcg acaatactga ttgcccgatc cacaccagcg cagaagccgc
     1921 gcggattggc aaggatgatg gttttctcgt tcataagccc ggtatttcgt tttcagacgg
     1981 catcaatatt tttcttcttg ggttttacgg tggacgatgt tgtccaacac tgccaacacc
     2041 gcaccgacgc agataaagct gtcggcaata ttaaaggcgg gataaaacca attttgccaa
     2101 taaaacaata agaaatcgac gacatgaccg tgtatcaggc ggtcgatgac attgcctaac
     2161 gcaccgccga taatcattgc cgcacccgtt ttgccgaggg ttgcaaactc atcgcgcaag
     2221 atggcgcgta ccaaatacgc gctcaccgtc accgccagca ccaaaaaaaa gtatttttgc
     2281 cagccgccct gatcggcaag gaagctgaac gccgcacccg ggttgtacac cagcgtcaaa
     2341 tcgaaaaagg aaggaatgac attgacgcgt tcccgatact gaaacgacga cagcaccgcc
     2401 cacttcgacc actggtccag cacgatggcg gcaagtgcca atacccaata gcgcgtttta
     2461 cttgaaacag atgaagacat atttttcaac agccggtaaa agagtaccat tttacccgaa
     2521 aacccccttt cctgtacccg aaacggcaaa tgccgtaatc ttaaaacccg tcattcccga
     2581 caacaccgta atctcgaaac ccgtcattcc cgcgcaggcg ggaatccaga cctgtccgca
     2641 cagaaactta tcggataaaa acagttgccc aaaccccgcg ttctatagtg gattaaattc
     2701 aaaccagtac ggcattgcct cgccttagct caaagagaac gattctctaa ggtgctgaag
     2761 caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgttgc cttgtcctga
     2821 tttaaattta a
//
LOCUS       DAXIEB010000159         2796 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000159 DAXIEB010000000
VERSION     DAXIEB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.159"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            36..473
                     /locus_tag="ACFOA3_001969"
     CDS             36..473
                     /locus_tag="ACFOA3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6898908.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            542..811
                     /locus_tag="ACFOA3_001970"
     CDS             542..811
                     /locus_tag="ACFOA3_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6898909.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            811..2586
                     /gene="ptsP"
                     /locus_tag="ACFOA3_001971"
     CDS             811..2586
                     /gene="ptsP"
                     /locus_tag="ACFOA3_001971"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6898910.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     IVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
BASE COUNT          713 a          807 c          759 g          517 t
ORIGIN      
        1 acgccggtag gacaacgagg aacaggacgc ataatatgat agggctttta atcatcacac
       61 acgaaaccat aggcgaagcc taccgcaagc tggcacatca tttttttccg ggcggactgc
      121 ctgaaaacgt ccgcatactc ggcgtgcaac cgacggaaga ccaagacgac atcatcaaca
      181 acgccattgc cgcgcttcag gagtttcccg acaacgacgg cgtgctgatt atgaccgata
      241 ttttcggcgc gaccccctgc aatgccgccc gccgcctcgt gcgcgaaaac aaatcggcga
      301 ttttgaccgg gctgaacgcg ccgatgatgg ttaaggccgt ccaatattcg ccggcggcgg
      361 aagaccttgc cgcctttacc gaatgcgtca gggaggcggc ggtaaaaggc attttcgcca
      421 tcacgtccgc gcccgaagat ttggtgtgcc ggcgcagcgg cgatgccgtc tgaagaagcg
      481 gcagggcagg aaaacatttt aaacggcgtc tgctgccgat atacataaca cgggaatcga
      541 aatgctcaaa caatccatcg aaatcatcaa caaactcgga cttcacgccc gtgcctccag
      601 caaattcacc caaaccgcgt ctcagtttca aagcgaagtc tgggttacga aaaacgacag
      661 ccgcgtcaac ggcaaaagca ttatggggct gatgatgctc gccgccgcca agggtacggt
      721 catcgaactg gagacggacg gcgcggacga ggcggaagcg atgcgcgccc tgaccgactt
      781 aatcaacggc tacttcggcg agggcgaata atgagtatcg tgctgcacgg cgtggcggcg
      841 ggcaaaggca ttgccgtcgg ttgcgcccac ctgattgcgc gcggtacgga ggaagtgccg
      901 cagtatgatg ttgcggaggc ggacaccgat gccgaagccg aacgtttcga tgccgccgtc
      961 aaagccacgc gcaaagagtt ggaacagctc cgcagcgcga ttcccgaaaa cgccccgacc
     1021 gagttgggcg cgttcatctc gctacacctg atgctcttaa ccgatgttac cttgtcgcgc
     1081 gaacccgtcg atattttaag ggaacaaaaa atcaacgccg agtgggcatt gaagcagcag
     1141 agcgacaaac tcgccgccca attcgacaat atggacgatg cctatttgcg cgaacgcaaa
     1201 caggatatgc tgcaagtcgt ccgccgcatc cacaacaacc tgatcgggca gggcaacgag
     1261 ttggaagttg ccgacaacct gtttgacgaa atcgtgctga ttgcaaacga cctttcgccc
     1321 gccgacacgg ttttgtttaa agagcagcgc attgccgcct tcgttaccga tgccggcggc
     1381 cccaccgggc atacggcgat tttgggcagg agcttggaca tcccgtccgt cgtcgggctg
     1441 cacaacgcgc gcaaactgat taccgagggc gaaacggtca ttgtggacgg tatcaacggc
     1501 gtgttgatta tcgcgccgga tgagtcggtg ttgaacgaat accgccgccg tgcccgcgaa
     1561 taccgcagcc acaaacgcga tttgaacaag ctcaaaaaaa ccgccgccgc caccgctgac
     1621 ggggtctgca tcgagcttgt gggcaatata gaatccgccg aagacgtgaa accgctgcac
     1681 aatctcggcg cagacggcat cgggctgttc cgcagcgagt ttctttacct gaaccgcgat
     1741 acgatgccgt ctgaagacga gcagtacgaa gtgtacagcg cgattgtcaa aaaaatgaag
     1801 ggcaaaagcg taacgatacg gacagtcgat ttgggtgtgg acaaaaaccc gcgctggttc
     1861 gggaaaaaca gcacgcccaa cggcagcctc aaccccgcgc tgggcatgac cggcatccgc
     1921 ctgtgccttg ccgaaccggt catgttccgc acccagatgc gcgccatcct ccgtgcggcg
     1981 gtacacggtc ccgtccgcat gatgtggccg atgattacct ccgtatccga agtgcgccag
     2041 tgcctcatcc acctcgacac cgcgcaacgc cagcttgccg aacgcggcga tgccttcggt
     2101 aaagtcggca tcggctgtat gattgaaatt ccgtctgccg cgctgaccgt cggcagtatt
     2161 ttgaaactgg tcgatttcat ctccgtcggt accaacgacc tgattcaata catcttgtcc
     2221 gtcgatcgcg gcgacgacag cgtcagccac ctctaccagc ccggccatcc cgccgtgctg
     2281 aaaatgctgc aacacgtcat ccgtaccgcc aaccgcatgg acaaagacgt atccgtatgc
     2341 ggcgagatgg cgggcgatac cgcgtttacc cgcgttttat tgggtatggg gctgcgccgt
     2401 ttttccatga accccaacaa catcctgccc gtcaaaaaca tcattctgca cagcaatgtc
     2461 gggcagctcg aaagcgatat tgtgaaagtc atccgctgcg aagacgaaga gaagtcggaa
     2521 aagctgatca aacagatcaa cagcgtgtct gtcgaggaag aagccgactt caaagggcgg
     2581 aaataaatac ggcaggtgaa aaatagaaat acttaacaat gcccgcaatc tgaaattttg
     2641 ccattcttgc aaaatagaaa accgaaacag aaacccaaaa tcggccattc cctcaaaaac
     2701 agaaaaccaa aatcagaaac ctaaaatccg tcattcccgc gcaggcggga atctaggtct
     2761 gtcggcacgg aaacttgtcg ggtaaaacgg tttctt
//
LOCUS       DAXIEB010000160         2788 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000160 DAXIEB010000000
VERSION     DAXIEB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.160"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            30..1247
                     /locus_tag="ACFOA3_001972"
     CDS             30..1247
                     /locus_tag="ACFOA3_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6898911.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1244..1375
                     /locus_tag="ACFOA3_001973"
     CDS             1244..1375
                     /locus_tag="ACFOA3_001973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898912.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1450..1590)
                     /locus_tag="ACFOA3_001974"
     CDS             complement(1450..1590)
                     /locus_tag="ACFOA3_001974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898913.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPAQAGAASDGI
                     LS"
     gene            1603..2400
                     /locus_tag="ACFOA3_001975"
     CDS             1603..2400
                     /locus_tag="ACFOA3_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6898914.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLTTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGAIWNGLPPAILSVSPVL"
     gene            2352..2729
                     /locus_tag="ACFOA3_001976"
     CDS             2352..2729
                     /locus_tag="ACFOA3_001976"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6898915.1"
                     /translation="MERLAARDIIRFPGFVNNVSEVIKAHHIFVLPSYREGVPRSTQE
                     AMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNAS
                     YAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          659 a          834 c          662 g          633 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac catcaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggcatatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgcagtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcacaggc tcaggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgat tctgcccgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcctcga catccatcgc catcatcgga ctggcaaaca
      841 tcgccggctg cattttttcc ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgacgatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttc gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcctgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg ataatgaatt ggatattatc
     1741 agggaaatgg gggtaacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatattt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgccgcaaa actggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcagccggaa
     1981 ggcataccgt taacaacaaa aatcataaag gggattttga ttgccttgta ccacattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggattcgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa attcctcttt
     2221 atcggcagat ttctgaaaga aaaggggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aaggacaaat accccgatac ggtttttacc gctttgggcg caatcgacaa atcacgcggg
     2341 gggggggcga tttggaacgg cttgccgccc gcgatattat ccgtttcccc ggttttgtga
     2401 acaatgtttc cgaagtgata aaggcgcatc atatattcgt attgccgtct tatagggaag
     2461 gcgttccccg aagcactcag gaggcaatgg ccgtcggcag ggcagtgatt acgacggatg
     2521 tccccggatg cagggaaacg gtcgccgaca aggtcaacgg cttcctgatc gagccttgga
     2581 atccccgcat cttagccgaa aaaatgattt attttatcga aaacagggaa gccgtccgcc
     2641 tgatggggaa tgcaagttat gcgattgcca aagataaatt cgatgccgaa aaagtcgatt
     2701 tgaaattgct cgatattttg aaggcgtaaa caaggctgcc cgcttttgag tttcggtcat
     2761 ttctgataaa cccccgtcat tcccgcca
//
LOCUS       DAXIEB010000161         2721 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000161 DAXIEB010000000
VERSION     DAXIEB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.161"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            412..924
                     /locus_tag="ACFOA3_001977"
     CDS             412..924
                     /locus_tag="ACFOA3_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6898916.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPEILLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            1004..2083
                     /gene="aroB"
                     /locus_tag="ACFOA3_001978"
     CDS             1004..2083
                     /gene="aroB"
                     /locus_tag="ACFOA3_001978"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6898917.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLHTLQTALDAAGVSHFSIILPDGEAYKNWQTLNLIFDGLMQHRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          673 a          809 c          635 g          604 t
ORIGIN      
        1 cagacagtac aaatagtacg gaaccgattc acttggtgtt tcagcacctt agagaatcgt
       61 tctctttgag ccaaggcgag gcaacgctgt actggttttt gttaatccac tatagtattg
      121 ataaacatat tatcttcaat atattcaatt ggataattgt ttacctaagc aaagataatt
      181 gccttttcct gacaaataag tgaaatcaac ggattgtcaa aacacagcct gaaataaaaa
      241 acctccctga tttcttttat ttgtccttaa aatcagaaag gttcgggatg gtcgggttat
      301 ttttccaaac gtaccgccgc cctgccgatt tcgtataaaa ttccgccgta acccgacaag
      361 cccgaaccct gtcgccccga aaggcggggt gtcaaacatt aaggaattgt gatgaaaaac
      421 tttaacggca aactcatcct catcggactg atgggcgcgg gcaaaaccac gctgggccgg
      481 caaatggcgc agcggctgga ttaccgtttt tacgacagcg atcacgaaat cgccgcagcc
      541 gccggcgttc ccatccccac catatttgaa atggaaggcg aacagggatt ccgttcgcgc
      601 gaaaccgcca tactcaaaaa gctggttatc ctgccccata tcgtcctgtc caccggcggc
      661 ggcgcggtgt taaaagaaga aaaccgcgcc cttatccgca aaagcggcac ggtcgtctat
      721 ctgcacgccc cgcccgaaat cctgctcgaa cgcacgcgct gcgacaacag ccgtcctttg
      781 ctgcaagttg ccgatccttt ggcgaaatta cgtgaactct acgccgcacg cgaccccgtt
      841 taccgccaaa ccgccgactt taccgtagaa tcggcaaact gccgggaaac cgtgcaaacc
      901 ctgctcaaac gcttatcccg ataaaccggc atatgcgccg cgcccagaaa accaaaccgc
      961 gccccgcccg gcgggccggc ggttcaaact ttaaggaaca acaatgaaaa cactgaccgt
     1021 acacaccccg tcccacagct accccatctt tatcggcaac gggctgctgc cgcaggcagg
     1081 cagcctgctc aaaccgcatt tgggcaaacg cgccgccatc atcaccaacg aaaccgtcgc
     1141 cccgctttat ctccacacgc ttcagacggc attggacgcg gcaggcgtgt cccatttcag
     1201 catcatcctg cccgacggcg aggcgtacaa aaactggcag acgctcaacc tcatctttga
     1261 cggtctgatg caacaccgcg ccgaacgcaa aaccacatta atcgcgctgg gcggcggcgt
     1321 tatcggcgac atggtcggct ttgccgctgc cacctaccag cgtggggctc cgttcatcca
     1381 aataccgacc acactgttga gtcaggtcga ttcctccgtc ggcggcaaaa ccgccatcaa
     1441 ccacccgctc ggcaaaaata tgatcggcgc gttctaccag ccgcaggcgg tgcttgccga
     1501 cttggacacg ctgcacaccc tgcccgtccg cgaattgtcc gccggtatgg cggaagtcat
     1561 caaatacggc gcgctcggcg acatcggttt tttcgaatgg ctcgaacaaa acatatccgg
     1621 actgatgacg ctcgatcggg aaaaactcgc ccaagccgtg taccgctgct gccaaatgaa
     1681 ggcagacatc gtcgcccaag acgaaaccga acagggcata cgcgcatggc tcaacctcgg
     1741 acacaccttc ggacacgcca ttgaaaccga gatgggttac ggcacttggc tgcatggaga
     1801 agccatcgcc gccggctgcg tgttggcggc gcgtttgtcc gaacaactgg gcaaaacctc
     1861 cgccgcagat accgcgcggc tcgccgccct gctcgaagcc gctggactgc cgtccgcacc
     1921 gcccgtgttt gcctttgaaa aatggctcga acatatgagc cacgataaaa aagtcagcgg
     1981 cggcatcatg cgctttatcg gtctgaaccg gctgggcgaa gccaacatca ccgaaattac
     2041 cgacacggac atcctccgcc gcaccctgca accgtatctc tgatttcctc tgccgatgtg
     2101 ctgccgcgcg ggtttgacgc acgatgatga ttttccatca tctttctccg taaaagcggg
     2161 aatccggtcc gttcggtttc ggtcgtttcc gataaattcc tgctgctttt catttctaga
     2221 ttcccacttt cgtgggaatg acggtggaga ggtttttgtt gtttcggaga agtttctgca
     2281 accctagaat ctcgttattt ccacaaaaaa cagaaaccca aaacagcaac ttaaaacctc
     2341 gtcattcccg caaaagcggg aatccagtcc gttcggtttc ggtcgtttcc gataaattcc
     2401 cgttgctttt catttctaga ttcccacttt cgtgggaatg acggcggaga ggtttttgtt
     2461 gtttcggaga agtttctgca accctagaat ctcgttattt ccacaaaaaa cagaaaccca
     2521 aaacagcaac ttaaaacctc gtcattcccg caaaagcggg aatccggtcc attcagtttc
     2581 ggtcgtttcc gataaattcc tgctgctttt catttctaga ttcccacttt cgtgggaatg
     2641 acggcggaga ggtttttgtt gtttcggaga agtttctgca accctagaat ctcgttattt
     2701 ccacaaaaaa cagaaaccca a
//
LOCUS       DAXIEB010000162         2655 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000162 DAXIEB010000000
VERSION     DAXIEB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.162"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..143)
                     /locus_tag="ACFOA3_001979"
     CDS             complement(<1..143)
                     /locus_tag="ACFOA3_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6898918.1"
                     /translation="MSTFFQQTSQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQRTR
                     YLR"
     gene            complement(367..2499)
                     /locus_tag="ACFOA3_001980"
     CDS             complement(367..2499)
                     /locus_tag="ACFOA3_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6898919.1"
                     /translation="MTAVPTPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          732 a          770 c          605 g          548 t
ORIGIN      
        1 tctctaaggt aacgggttct ttgacgattc aggtactgtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaatcat ggcttgggag
      121 gtttgctgga agaaggtgct catggaaaat cccctaaatg gcttggtggg aatttagggg
      181 attttggggg attttgcaaa ggtctctaga tgagtgaaaa agaagtgcag gctgcctaaa
      241 aagacagaaa aagtctttcc ggcagcctgc actttggttt catttcagtc agtaaaccca
      301 gtaaacgacg gtctgaaaac gcagaacgtt acgaaaaaag cagcctacac gcccatcccc
      361 cgccttctac ccgttctgta aatcatacag atagcggtaa tatccgttcg gcttcgccag
      421 caattcctgc tgtgttcccg cttccacaat cctgccttta tccatggcaa tgattcggtg
      481 tgccgtttta acggtggaca gacggtgggc gataatcagc accgtccggt tggcgcaaat
      541 ggcctgcatg ttctgcataa tcgctcgttc actttcataa tccagcgcgc tggtggcttc
      601 atcaaaaatc agaatgcgcg gattggtgat taacgcgcgg gcaatcgcaa tacgctgccg
      661 ctgtccgccc gacaagccgg ccccttgttc gcccaccacg gtgccgtagc cttccggcag
      721 ctccataata aactcgtgtg cgcccgccag tttggctgct tcgataatgc gttccagcgg
      781 catacccgta tccgtcagcg cgatattgtc gcgtatgctg cggttgagca gcacattctc
      841 ctgcaagacc acgccgacct gccgccgcag ccaggcagga gcggccaaag ccaaatcgtt
      901 gccgtccacc aacacccgtc cctgctccgg tacatacaga cgctgcacca atttggtgag
      961 tgtggatttg cccgaccccg aacgtcccac aatccccagc acttcccccg cccgaatccg
     1021 caggttcaaa tcctgcaaaa tcagcctgcc gtccgcctta tagcggaaat cgacatgttc
     1081 gaacgtaatc tccccccgga tatcgggcaa agccaaatgc gaagacgcat tctcggtcgg
     1141 cgcattcaga atatccccca aacgcgccac cgaaatcccc acctgctgga aatcctgcca
     1201 caactgcgcc aaacggataa caggcgccgc cacctgtccc gagagcatat taaacgcaat
     1261 cagctgcccc accgtcagct tgccctcaat taccagccgt gcgccaatcc acaacgtcgc
     1321 caccgtcacc agcttctgaa tcagctgcac cccctgctgg ccgaccaccg ccaacttcgt
     1381 tacccgaaat cccgaagcca cataagccgc caactgattg tcccaacgct gcgtcatctg
     1441 cggctccacc gccatcgcct ttaccgtacc caccgcagtg atgctttcta ctaaaaacga
     1501 ctggttgtct gcattgcgcg cgaacttatc gttcagacgc gtccgcagta tcggactgat
     1561 aaatgccgac caaaacgcat aggcaggcaa cgaagccaat accacccaag tcagagtgga
     1621 gctgtaatac cacatcaccg ccagaaagat aaacgaaaac gccaaatcca acaccgaagt
     1681 cagcgcctga ccggtcaaga aattgcgaat ctgctccaat tcccgcaccc gagccaccgt
     1741 atcacccact cgtctgtgct cgaaatagga taaaggcagg gaaagcagat gccggaacaa
     1801 acgcgcgccc aattccacat caatacgtga agtcgtatgt gcaaacagat acgtccgcaa
     1861 accgcccaac acaatctcaa acagcgacac caccaacaaa gccaccgaca ccacatccaa
     1921 agtagagaat ccccgatgta ccagcacctt gtccatcacc acttggaaaa acagaggcgt
     1981 aatcagcgca aacagctgca acaccaccga caccaccaat acttcaaaaa acaaccggcg
     2041 gtatttgatt accgccggaa taaaccaggt aaagtcaaac tttgccaaac tgcccaatac
     2101 cgaagcgcgg gaagcaacca atatcagttt gcccgaatat ctgttagaaa attcggcaaa
     2161 agacaatacc gcagacttat tcgtaaccaa atcctgtatc aaaaattggg catgctcacc
     2221 ctcaccgtct gttttggcca aaatgaaatg gttgccgtca tcacaccata ccaatgcggg
     2281 taaagtcgcc atagccaaac gtttaatagg ctggcggact acctttgcct tcaatcccaa
     2341 agatttggcg gctaacagcc attgcgtttc atttaaatcg ctctgtgcgg aagtacaaaa
     2401 ttcatgctgt atatcggcag gattggcggc aatgccgtgg taatgggcga ggatgatgag
     2461 ggcggaaagg gcggggagag gtgtgggtac ggcggtcatg atattgagat tgaataacgg
     2521 gtaacaaaaa atataccaaa aatagcaaaa caaattgtaa ggatacgtat aggctttgta
     2581 aaggtaaatt gtgaaaaaag cagtttttta aacgaatgaa acggcttcgg gctgaaatat
     2641 atgctgatgc cctgt
//
LOCUS       DAXIEB010000163         2620 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000163 DAXIEB010000000
VERSION     DAXIEB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.163"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            77..640
                     /gene="gmhB"
                     /locus_tag="ACFOA3_001981"
     CDS             77..640
                     /gene="gmhB"
                     /locus_tag="ACFOA3_001981"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6898920.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            670..1413
                     /locus_tag="ACFOA3_001982"
     CDS             670..1413
                     /locus_tag="ACFOA3_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6898921.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETV"
     gene            1410..2099
                     /locus_tag="ACFOA3_001983"
     CDS             1410..2099
                     /locus_tag="ACFOA3_001983"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6898922.1"
                     /translation="MTSLIHTLSDGMELTIEIKRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPQTAENRLPESILFHGRQLALTAHQDTQILLMPS
                     EIRVPEGAPEKQLALLQDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWL
                     KIHGRELFALG"
BASE COUNT          630 a          876 c          628 g          486 t
ORIGIN      
        1 acgcggcaca ggcgcatcac tgccgatatt tcagtataat gccacccccg acctgcccca
       61 atccaaagga aacgcgatga aactcatcat tctcgaccgc gacggcgtca tcaatcagga
      121 ccgcgatgac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca gcatggatgc
      181 ggtggcattt ctgacgcagg caggctacac cgtcgccgtt gccaccaacc aatccggcat
      241 cgggcgcaaa tattttaccg ttcaaaacct taccgaaatg cacgccaaaa tgcaccgcct
      301 cgtccgtcag gcaggcggcg aaatcaacgg catctggttc tgcccgcaca ccgatgccga
      361 caactgcaac tgccgcaagc ccaaaccggg tatgattgaa gacatcatcg gacgcttcaa
      421 cgcccaagcc tcggaaacct ggctggtcgg cgacagcctg cgcgatttgc aggcaatcga
      481 tgccgtcggc ggcaaacccg cgctggttct gaccggaaaa ggcaaaaaaa cgctctccca
      541 acacggacac gaattacccg aacacacaca ggttttcgat accttgctcg atttctcaca
      601 atacatcatg caggaaaacg ccgcaccgca agccgactga acataccgca tttccgacaa
      661 ggcaaaacca tgctcatcat ccgcaacctg atttactggc tgatactctg ttccaccctg
      721 attttcctct ttccctttat gctgctcgcc tcgcctttcc gggacggggc gcacaagatg
      781 gcgcgggtct gggtcaaaat cctcaacctc tcgctcaaac acatcgtcgg gctcaaatac
      841 cgcatcatcg gcgcggaaaa catccccgac cgccccgccg tcatctgcgc caaacaccaa
      901 agcggctggg aaacgcttgc ccttcaggac atttttccgc cgcaggttta cgttgccaaa
      961 cgcgagctgt tcaaaatccc ctttttcggc tggggtttaa aactggtcaa aaccataggc
     1021 atagaccgca acaaccgccg cgaagccaac gaacagctca taaaacaggg gttggcgcgc
     1081 aaaaacgaag gctattggat taccattttc cccgaaggca cgcgccttgc gcccggaaaa
     1141 cgcggcaaat acaaactcgg cggcgcgcgc atggcgaaaa tgtttgagat ggacatcgtc
     1201 cccgtcgccc tcaacagcgg cgaattttgg ccgaaaaact cctttctgaa atatccgggg
     1261 gaaatcaccg tcgtcatctg tccgaccatc ccgcacgcaa gcggcagcga agccgaattg
     1321 atggaaaaat gcgaacacct catcgaaacg cagcagccgc tcatttccgg cgcaggcccg
     1381 tttgccgcca aaatgccgtc tgaaaccgta tgacctccct gatccacact ctttctgacg
     1441 gcatggaact gactatagaa atcaagcgcc gtgccaagaa aaacctgatt atccgcccta
     1501 tcggcacaca taccgtccgc atcagcgtcc caccctgctt ctccgtctcc gctctaaacc
     1561 gctggctgta tgaaaacgaa gccgtcctgc ggcgaacact ggcgaaaaca ccgccgcaaa
     1621 ctgccgaaaa ccggctgccc gaatccatcc tcttccacgg cagacaactt gccctcaccg
     1681 cccatcaaga cacgcaaatc ctgctgatgc cgtctgaaat ccgcgttccc gaaggcgcac
     1741 ccgaaaaaca gcttgcgctg ctgcaggact ttttggaacg gcaggcgcac agttacctga
     1801 ttccccgcct cgaacgccac gcccgcacca cacaactgtt ccccgcctcc tcctcgctga
     1861 cctctgccaa aaccttctgg ggcgtgtgcc gcaaaaccac aggcatacgc ctcaaccggc
     1921 ggctggtcgg cgcaccggaa tacgttgccg attatgtctg catacacgaa ctctgccacc
     1981 tcgcccatcc cgaccacagc cccgcctttt gggaactgac ccgccgcttc gccccctaca
     2041 cgcccgaagc gaaacagtgg ctcaaaatcc acggcaggga acttttcgcc ttaggctgac
     2101 gcggtcccgg gccggcatcc gccaattcag acctcctccc aaacggacat ccggaaagca
     2161 gcatcacagc cggcgacaca taccgctgcc gccgctttca gacggcatcc gtgccggaac
     2221 aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg ccgccggttc ggggcaggat
     2281 ggcggcacac gcgccgtctg ccgcgtttca tttcacaccg ctcttccgaa acccgaaacc
     2341 cgcccggtcc gacgtgcggt atgaaacgct taagctgacg cgaagtcttt tactgatttg
     2401 cccgcgaaaa tgccgtctga aaggttttcg gacggcattt ttttgcgttt cccaggatgg
     2461 cggcggattc gtaaaaggcg gtcagggtgg attgtaggat gggttgagac ctgccgaatc
     2521 cgccgcatct gccaaatcta ccgccgtcat tcctacgaaa gtgggaatct agaacgcggg
     2581 gttaagaaaa cctgcatccc gtcattccca cgaaagtggg
//
LOCUS       DAXIEB010000164         2581 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000164 DAXIEB010000000
VERSION     DAXIEB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.164"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(196..267)
                     /locus_tag="ACFOA3_001984"
     tRNA            complement(196..267)
                     /locus_tag="ACFOA3_001984"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Arg,seq:cct)
     gene            complement(287..733)
                     /locus_tag="ACFOA3_001985"
     CDS             complement(287..733)
                     /locus_tag="ACFOA3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6898923.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFOA3_001986"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFOA3_001986"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6898924.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="ACFOA3_001987"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="ACFOA3_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6898925.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFOA3_001988"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFOA3_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6898926.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          755 a          697 c          575 g          554 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgga gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgcaaatcg tcaaacctcg ccaacaggaa tcgaacctgt attttacgct taggaggcat
      241 acgttctatc cgttgaacta tggcgagccg aaatgaaaag gagattttaa ccctttccga
      301 cgacaaaaga caagatgccg gccgcaatca gcggcccgac agggataccg cccataaatg
      361 ccaccccgat aaccgtcccg attaacagcc ctgtaattaa aacaggctgc tgtcccatca
      421 aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg gcggatatca
      481 ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa accaacggac
      541 tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccgtgcttc tcgaccaacg
      601 ggacaaactg taccaatgcc gtctgctgca tcagcagcaa tatggttgcc gagatggtaa
      661 tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac aggggaacaa
      721 aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaacgcgg caacggcaca atcgactgct
      841 ttgcgcgcct cgcctatcga atatgccaac gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtgccgac gtacataccg tagtctttca acgcctgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggataa cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagggtg gaagtgtgga taaactcgac catcgccgcc
     1501 agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcca
     1801 tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgaactgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
     2581 c
//
LOCUS       DAXIEB010000165         2527 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000165 DAXIEB010000000
VERSION     DAXIEB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.165"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            45..2258
                     /locus_tag="ACFOA3_001989"
     CDS             45..2258
                     /locus_tag="ACFOA3_001989"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6898927.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          671 a          795 c          604 g          457 t
ORIGIN      
        1 gctgttacaa taaactcgtt tttatcggaa cggaagaccc catcatgacc gccatcagcc
       61 cgattcaaga cacgcaaagc gcgactctgc aagaattgcg cgaatggttc gacagctact
      121 gcgccgcgct gccgaacaac gataaaaaac ttgtcttagc cgcccgttcg ctggcggaag
      181 cacattaccc cgccgatgcc gccacgccgt atggcgagcc gctgcccgac cacttcctcg
      241 gcgcggcgca aatggttcat gaactcgacc tgctccccga tgccgtcgcc gccaccctgc
      301 ttgccgacat cggacgctac gtccccgatt ggaacctatt ggtttccgaa cgctgcaaca
      361 gtaccgtcgc cgagctggtc aaaggtgtgg acgaagtgca gaaactcacc cacttcgccc
      421 gggtggacag cctcgccacg ccagaagaac gcgcccagca agcggaaacc atgcggaaaa
      481 tgctgctggc gatggttacc gacatccgcg tcgtgttaat caaactggcg atgcgtacgc
      541 gcaccctgca atttttaagc aacgcccccg acagccccga aaaacgcgcc gtcgccaaag
      601 aaaccctcga catcttcgcc ccgctcgcca accgtttggg cgtgtggcag ctcaaatggc
      661 agctcgaaga tttgggcttc cgccatcaag agcccgaaaa ataccgcgaa atcgcgctgc
      721 ttttggacga aaaacgcacc gaacgcctcg aatacatcga aaacttcctc aacatcctgc
      781 gcggtgaact caagaaatac aatgtccatt tcgaagtcgc cggccgcccg aaacacatct
      841 actccattta caaaaaaatg gtgaagaaaa aactcagctt cgacggcctc tttgacatcc
      901 gcgccgtgcg gattctggtt gataccgtcc ccgagtgtta caccacgctg ggcattgtcc
      961 acagcctttg gcagcctatc cccggcgagt tcgacgacta catcgccaac ccgaaaggca
     1021 acggctataa aagtttgcac accgtcatcg tcggtcccga agacaaaggt gtggaagtgc
     1081 aaatccgcac cttcgatatg caccaattca acgaattcgg tgtcgccgcc cactggcgtt
     1141 acaaagaagg cggcaaaggc gattccgcct acgagcaaaa aatcgcctgg ttgcgccaac
     1201 ttttggactg gcgcgaaaat atggcggaaa gcggcaagga agacctcgcc gccgccttca
     1261 aaaccgagct gttcaacgac acgatttacg ttttaacccc gcacggcaaa gtcctttccc
     1321 tgcctacggg cgcgacaccc atcgacttcg cctacgccct gcacagcagc atcggcgacc
     1381 gctgccgggg cgcgaaagtc gaagggcaaa tcgtgccgct gtccaccccg cttgaaaacg
     1441 gacagcgcgt cgaaatcatt accgccaaag aagggcatcc ttccgtcaac tggctgtacg
     1501 aaggctgggt caaatccggc aaggcaatca gtaaaatccg cgcctacatc cgccagcaaa
     1561 acgccgacac cgtgcgcgaa gaaggccgcg tccaaatcga caagcagctt gccaaactca
     1621 cgcccaagcc caacctgcaa gagcttgccg aaaacctcgg cttcaaaaaa ctcgacgacc
     1681 tctacactgc cgtcggacaa ggcgaaattt ccaaccgcgc catccaaaaa gcctgcggca
     1741 cgctgaacga acctccgccc gtacccgtca gcgaaaccac catcgtcaaa cagtccaaaa
     1801 tcaaaaaagg cggcaaaaac ggtgtgctca ttgatggcga agacggtttg atgaccacgc
     1861 ttgccaaatg ctgcaaaccc gcgccgcccg acgacattgt cggcttcgtt acccgcgatc
     1921 gcggcatttc ggtacaccgc aaaacctgcc cctctttccg acacctcgcc gaacaagcac
     1981 ctgaaaaagt gctggacgca agctgggcgg cgttgcagga aggacaagtg tttgccgtcg
     2041 atatcgaaat ccgcgcccaa gaccgctctg ggcttttgcg cgacgtatcc gacgcgctcg
     2101 cccgccacaa actcaacgtt accgccgtgc aaacccagtc ccgcgacttg gaagccagca
     2161 tgaggttcac gctcgaagtc aaacaagtta ccgacctccc acgcgtcctc gccagcctcg
     2221 gcgacgtcaa aggcgtattg agcgttaccc ggctttaaat acaaaaatgc cgtctgaaag
     2281 ccgtataacg cttcagacgg cattttgatt gccggggttt gctatttttt gttgcataat
     2341 caattaaaaa caaaatagta caatattcaa ctttgaaggt ctaaccatgg catactctgc
     2401 ggacttaaga aacaaagctt taaactatag tggattaaca aaaatcagga caaggcgacg
     2461 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa caccgtactg gtttaaattt
     2521 aatccac
//
LOCUS       DAXIEB010000166         2513 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000166 DAXIEB010000000
VERSION     DAXIEB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.166"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            242..862
                     /locus_tag="ACFOA3_001990"
     CDS             242..862
                     /locus_tag="ACFOA3_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6898928.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            885..2336
                     /locus_tag="ACFOA3_001991"
     CDS             885..2336
                     /locus_tag="ACFOA3_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6898929.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT          559 a          583 c          594 g          777 t
ORIGIN      
        1 aatttccatt ttaaaacaat tagtaaaatc gagtttatct tagttgtcca ggacagcccc
       61 attattttta taacaccgtg aagccgcaca gcagtttgaa cagtgatacg ccgtttgcgg
      121 gcttacgagt ttattttccc ggcctgcagt ttgagcaata cggtgatttc ctacggttaa
      181 tacaaatgtt tacacattga tacatttcat ttatagttcc gcctatttga aaatagaaaa
      241 tatgaattcg accgcaagta aaaccctgaa aggattgtcg ctggtgtttt tcgcctctgg
      301 attctgcgcc ctgatttacc aggtcagctg gcagaggctt ctattcagtc acataggtat
      361 cgatttgagt tcgattactg tcattatttc tgtatttatg gtcggcttgg gtgtaggtgc
      421 gtatttcggt ggacgcattg ctgaccgttt tccttcaagt atcatccccc tgttttgcat
      481 cgctgaagta tccatcggtc tgttcggttt ggtaagcagg ggtctgattt ccggcttggg
      541 gcatctttta gttgaggctg atttgcccat catcgctgct gccaatttcc tcttattgct
      601 gcttcctacc tttatgatgg gcgcgacctt gcccttgctg acctgttttt ttaaccggaa
      661 aatacataat gttggcgagt ctatcggtac cttatatttt ttcaacactt tgggtgcggc
      721 actcggatcg cttgccgccg ccgaattttt ctacgtcttt tttaccctct cccaaaccat
      781 tgcgctgaca gcctgcttta accttctgat tgctgcttca gtatggctgc gttacagaaa
      841 ggatggatat agtgaacact aaaccgaata ctagtttgat ttatatgctt tctttcctta
      901 gcggcttatt gagcttgggt atagaagtct tgtgggtaag gatgttttcg ttcgcagcac
      961 agtccgtgcc tcaggcattt tcatttactc ttgcctgttt tctgaccggt atcgccgtcg
     1021 gcgcgtattt tggcaaacgg atttgccgca gccgctttgt tgatattccc tttatcgggc
     1081 agtgcttctt gtgggcgggt attgccgact ttttgatttt gggtgctgcg tggttgttga
     1141 cgggtttttc cggcttcgtc caccacgccg gtatcttcat taccctgtct gccgtcgtca
     1201 gagggttgat tttcccgctc gtacaccatg tgggtacgga tggcaacaaa tccggacgac
     1261 aggtttccaa tgtttatttc gccaacgttg ccggcagtgc attgggtccg gtccttatcg
     1321 gctttgtgat acttgatttc ttgtccaccc aacagattta cctgctcatc tgtttgattt
     1381 ctgctgctgt ccctttgttt tgtacactgt tccaaaaaag tctccgactg aatgcagtgt
     1441 cggtagcagt ttccctaatg ttcggcatcc tcatgttcct actgccggat tctgtctttc
     1501 aaaatattgc tgaccgtccg gataggctga ttgaaaacaa acacggcatt gttgcggttt
     1561 accatagaga tggtgataag gttgtttatg gggcgaatgt atacgacggc gcatacaata
     1621 ccgatgtatt caatagtgtc aacggcatcg aacgtgccta tctgctaccc tccctgaagt
     1681 ctggcatacg ccgcattttc gtcgttggat tgagtacagg ttcgtgggcg cgcgtcttgt
     1741 ctgccattcc ggaaatgcag tcgatgatcg ttgcggaaat caatccggca taccgtagcc
     1801 ttatcgcgga cgagccgcaa atcgccccgc ttttgcagga caaacgtgtt gaaattgtat
     1861 tggatgacgg taggaaatgg ctgcgtcgcc atcctgatga aaaattcgac ctgattttga
     1921 tgaatacgac ttggtactgg cgtgcctatt ccaccaacct gttgagtgcg gaatttttaa
     1981 aacaggtgca aagccacctt accccggatg gtattgtaat gtttaatacc acgcacagcc
     2041 cgcatgcttt tgctaccgcc gtacacagta ttccctatgc ataccgctat gggcatatgg
     2101 tagtcggctc ggcaaccccg gtagttttcc ctaataaaga actgctcaag caacgtctct
     2161 cccggttgat ttggccggaa agcggcaggc acgtatttga cagcagcacc gtggatgctg
     2221 cagcacaaaa ggttgtctct cgtatgctga ttcagatgac ggaaccttcg gctggggcgg
     2281 aagtcattac cgacgataat atgattgtag aatacaaata cggcagaggg atttaaccgt
     2341 cttaaagggt ttcaggcaac gcaggtttta ggtaacgtcc tgctagttca aaaaaaaccg
     2401 catcacagca gtcgggacaa aatggtttaa acattttgtc ccgaattctt attcctatat
     2461 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aga
//
LOCUS       DAXIEB010000167         2500 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000167 DAXIEB010000000
VERSION     DAXIEB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.167"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..805
                     /locus_tag="ACFOA3_001992"
     CDS             <1..805
                     /locus_tag="ACFOA3_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6898930.1"
                     /translation="NIQITDEAAQIRLTGKTAAQTKADIGTNVTTDTAERHSGSLKNT
                     FNREAVQNELDLQREVTQEFDKIRQGVKQELYAVVDSKRAQATQERLKNGGYDNDKSR
                     ALNKEANELDEKIRWLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHK
                     KIIDEKTGKVSFDTRQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAA
                     KQNRNSTNGSGVIAVMNPPTGKYKSDSNNKMKDFLWLGSSLVSELMYVG"
     gene            824..1462
                     /locus_tag="ACFOA3_001993"
     CDS             824..1462
                     /locus_tag="ACFOA3_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6898931.1"
                     /translation="MFQGYLPKTNSEKLNQDIYREVQKMGNGWSVDTSNHSRGGITAS
                     VSLKDWVNNQKQNGIAPIRKARFYGTATNVQNDYADVLQKNGYTYTGADGKIYNSGAY
                     SIVHDKDFVGNKWIPFLLGNNETTKGACKGFCYSHSSYFAEVPEQYQRDRNGEYILDK
                     NNNKIETEDWKDYKGKWGVPKMGDDGKYINHAIPKLVYPNKLNGEQYEENPF"
     gene            1443..1760
                     /locus_tag="ACFOA3_001994"
     CDS             1443..1760
                     /locus_tag="ACFOA3_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898932.1"
                     /translation="MKKTRSNMVLILFCSTILTGCFGSCGDWNCSGNPRWNEKVNDCY
                     SRNLEVVKQQLGYKKYGGDIVRFHSEVQDSEIGQHISTQCIYYSGKYDIHKDKQYGHL
                     FVK"
     gene            1926..2177
                     /locus_tag="ACFOA3_001995"
     CDS             1926..2177
                     /locus_tag="ACFOA3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898933.1"
                     /translation="MFDGSCADWNCSKPTQRWAKAVDTCETENLTIVLQVLGEDLGYK
                     SLVEVQVSEIGQHISGQCTYYDGRYDVRKDKQYGHLFVQ"
     gene            2343..>2500
                     /locus_tag="ACFOA3_001996"
     CDS             2343..>2500
                     /locus_tag="ACFOA3_001996"
                     /inference="COORDINATES: protein motif:HMM:NF014813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6898934.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILP"
BASE COUNT          877 a          425 c          537 g          661 t
ORIGIN      
        1 caacattcaa atcaccgacg aagccgcaca aatcaggctg acaggcaaaa cagcggcaca
       61 aaccaaagcc gatattggta caaacgtaac cacagacacc gccgaacgac attcgggcag
      121 cttgaagaac accttcaaca gagaagcggt gcaaaacgag ctggatttgc aaagggaagt
      181 aacccaagag tttgataaaa ttagacaagg agtgaaacaa gaactttatg cagtagtaga
      241 tagcaaacga gcgcaagcca cacaagagag actcaaaaat ggtggatatg acaatgataa
      301 atctagagca ttaaataagg aagctaatga attagatgaa aaaattcgtt ggcttgatgc
      361 aggattagga ctcgtttggg gcgcagggag ttctgatatg gcttggagta tgtttgccac
      421 aacccaagcc gatagagcgg taaggtctgc aactgcacct aaagaaatgt ggttccataa
      481 gaagataata gatgaaaaaa caggtaaagt atcctttgat accagacaaa tttggtcatt
      541 gaatgattta agcaaggaag aactggcaag cattcaagac acaaatggca aagttattac
      601 tgtgtctaat cctggtattt tcaataatcg agaagattca ttaagcaacg cagcaaaaca
      661 aaatcgtaat agtacaaacg gtagtggtgt tattgcagtt atgaatcctc caacagggaa
      721 atataaatct gattctaata acaaaatgaa agatttttta tggctcggtt caagtcttgt
      781 ttctgaactg atgtatgtcg gttaagacca attaaataat aaagtgttcc aaggctattt
      841 acccaaaacc aattcagaaa aactgaatca agatatttat cgagaggttc aaaaaatggg
      901 taatggttgg tcggttgata ccagtaatca cagtcgtggg ggaattacag caagcgtttc
      961 cttaaaagat tgggtaaaca atcaaaaaca aaatggcatt gccccaatca gaaaagcacg
     1021 tttctatggt acagccacaa atgtgcagaa tgattacgcc gatgttttac agaaaaacgg
     1081 ctatacctat acgggtgcag acggcaaaat ttataacagc ggagcctatt cgattgtgca
     1141 tgataaagat tttgtgggga acaaatggat acctttcttg ctaggaaata atgagacaac
     1201 aaaaggagct tgtaagggat tttgctattc gcatagcagt tattttgcag aagtgcctga
     1261 gcagtatcaa agggatagga atggtgaata tatccttgat aagaacaaca acaaaataga
     1321 aacagaagat tggaaagact ataaggggaa atggggtgtt cctaaaatgg gtgatgacgg
     1381 caaatatatc aatcatgcta ttccgaaatt agtgtatccg aataaactca atggtgagca
     1441 atatgaagaa aacccgttct aatatggtat tgatactgtt ttgctcaaca atattaactg
     1501 gctgttttgg tagttgcggg gactggaatt gttcaggtaa tccgcgatgg aacgaaaaag
     1561 ttaatgattg ttatagtaga aaccttgaag ttgttaagca acaattagga tataaaaaat
     1621 acggtggtga tattgttcgt ttccatagcg aagtgcaaga tagcgaaatt ggacaacata
     1681 tctctacgca atgtatttac tatagcggta aatatgatat tcataaagat aagcagtatg
     1741 gtcatttgtt tgtgaaatag ctagtaatga tagcaaatac ataaatcacg ctattccaaa
     1801 attagtcaat cccaataatc cgaatggaga aaaatatgaa acaaatcctt tttaaatatt
     1861 ttatgctaat tacttgtgtt tttggattaa gcagttgtgg ttatttatta tcatttgggc
     1921 agttaatgtt tgatggcagt tgcgcagatt ggaattgctc caagcctact caaagatggg
     1981 caaaagccgt tgacacatgt gaaactgaaa atctcacaat agtgttacaa gtattagggg
     2041 aagatttagg atacaaatct ctcgttgagg tacaagtcag tgaaattggg caacatattt
     2101 ctggacaatg tacttactat gatggcagat acgatgttcg caaagataag cagtatggtc
     2161 atttgtttgt gcaataacca gcgtagcctg tgcaattaat acgaactgtt acatggtagg
     2221 gtgggcaact cgttgcccac gcagttcagt cccatgtgct aaaactatgt tttaacgtga
     2281 aatatttcat ttcaggctga aaccattttt tgttttaacc aacacgaacc ctaacttaaa
     2341 atatgcccat cactccaccc ttaaacatca tctcccctaa actctacccc aatgaacaat
     2401 ggaacgaaag cgaagcactc ggtgccatca cttggctatg gtatcagtcg cctacgcatc
     2461 gccaagtacc tattgtggag atgatgacgt atatattgcc
//
LOCUS       DAXIEB010000168         2483 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000168 DAXIEB010000000
VERSION     DAXIEB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.168"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            714..1166
                     /gene="dut"
                     /locus_tag="ACFOA3_001997"
     CDS             714..1166
                     /gene="dut"
                     /locus_tag="ACFOA3_001997"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6898935.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1243..2430
                     /gene="dapC"
                     /locus_tag="ACFOA3_001998"
     CDS             1243..2430
                     /gene="dapC"
                     /locus_tag="ACFOA3_001998"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6898936.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
BASE COUNT          601 a          599 c          634 g          649 t
ORIGIN      
        1 aggtctagat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa cctgcaccac
       61 gtcattccca cgaacctgca tcccgtcatt cccacgaaag tgggaatcca gttttttgag
      121 tttcagtcat ttccgataaa ttgccttagc attgaatgtc tagattcccg cctgcgcggg
      181 aatgacgcat ccatccgtac ggaaacctgc accacgtcat tcccacgaaa gtgggaatcc
      241 agttttttga gtttcagtca ttttcaataa attgccttag tattgaatgt ctagattccc
      301 gcctgcgcgg gaatgacgga ttttaggttg gggtcattta ttgggaaaag cagaaaccgc
      361 tccgccgtca ttcccacgaa agtgggaatc tagaaattta atgttgcggc actagccaaa
      421 aaaaccgaaa ccgaacggac tagattcccg cctgtgcggg aatgacgaat ccatccatac
      481 ggaaacctgc accacgtcat tcccacgaaa gtgggaatcc agttttttga gtttcagtca
      541 ttcccgataa attgccttag tgtgtgcata atgttcgtct tcataacggg aagggcgggc
      601 attatacact ggcaacggat ttcaaaacaa aaccgatttg ccgtgtttca gcgtaaacac
      661 ggcttgtgta taatctccca tctttgaaac cggccgtatg caggagcaag acgatgaata
      721 ttgaagtaga aatgaaagta ttggacgaac ggatggcgga tgttgtccct gtctatgcaa
      781 cggagggttc tgcaggttta gatttgcgcg cctgtttgga tgaggaagtc gtcttgcagc
      841 cgggtgaaac gtttcttgta ccgacaggtt tggcaattta tttggcgaat cccgcatatg
      901 ccgccgtttt gctgccccgt tccggcttgg ggcataaaca cggcattgtt ttgggcaatt
      961 tggtcggctt gattgactcc gattatcaag gggaattgaa ggtttcgtta tggaacagaa
     1021 gcagcgagcc gtttactgtc aaaccgtttg agcgtatcgc gcagatggtt atcgtgccaa
     1081 tcgtgcaggc gcgtttcaaa cgtgtcgagg aatttgtcgg aagcagccgg ggtgagggcg
     1141 gcttcggcag tacgggtttg cattgaatat aaaatgccgt ctgatgggtg ccgtcaggtt
     1201 cagacggcat atcttccgat ataccgataa ccagagaaaa tcatgaatac cttactcaag
     1261 cagctcaaac cctatccctt tgcccgactg cacgaggcga tgcagggcat ttccgcgcct
     1321 gaaggcatgg aagccgtccc actgcatatc ggcgaaccga aacatccgac accgaaagtc
     1381 attacggatg cgctgaccgc ctcattgcac gagttggaaa aatatccgct gacggccggt
     1441 ttgcctgaac tgcgtcaggc gtgtgcgaac tggttaaaac gccgttatga tgggttgacg
     1501 ctgaattccg acaccgaaat tctgccggtt ttaggcagta gggaggcgtt gttttctttt
     1561 gtccaaaccg tgttgaaccc tgtttcagac ggcctcaaac ccgcaattgt cagcccgaat
     1621 cccttttatc agatttacga aggtgcgaca cttttgggcg gcggtgaaat ccattttgcc
     1681 aattgccccg cgccgtcttt caaccccgat tggcgtagta tttccgaaga ggtttggaaa
     1741 cgcaccaaac tggtgttcgt ctgctcgccc aacaacccca gcggcagcgt gctggatttg
     1801 gatggatgga aagaagtttt tgatttacag gataaatatg gtttcattat tgcctcggat
     1861 gaatgctatt ccgaaatcta tttcgacggc aacaaacctt taggctgcct gcaggccgct
     1921 gcacagttgg ggcgaagcag gcaaaaactg cttatgttca ccagtttgtc caagcgttcc
     1981 aacgttccgg gcctgcgttc cggttttgtc gccggcgatg ccgaactgct taaaaacttt
     2041 ctgctttaca gaacctatca cggcagtgca atgagcattc ccgtgcagcg cgcaagcatt
     2101 gccgcttggg atgacgaaca gcacgttatc gacaaccgcc gcctgtatca ggaaaaattt
     2161 gagcgcgtta ttcccatttt gcaacaggta tttgacgtta aattaccgga tgcctcgttt
     2221 tacatctggt tgaaagtccc cgatggcgac gatttggcat ttgcacgcaa tttatggcaa
     2281 aaagccgcta tccaagtatt gcccggacgt tttttggcac gggataccga acagggcaat
     2341 cccggggaag gttatgtccg tatcgctttg gttgccgatg tcgcaacttg tgtcaaagct
     2401 gcggaagaca tagtttcact gtatcggtaa ggaatcggaa atgccgtctg aacttttgtt
     2461 cagacggcat ttttcaatat tct
//
LOCUS       DAXIEB010000169         2468 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000169 DAXIEB010000000
VERSION     DAXIEB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.169"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(13..819)
                     /locus_tag="ACFOA3_001999"
     CDS             complement(13..819)
                     /locus_tag="ACFOA3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6898937.1"
                     /translation="MPTVFIFLMAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLSGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="ACFOA3_002000"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="ACFOA3_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6898938.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          678 a          627 c          642 g          521 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacagct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcaaca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagtgat gtgccgatat gcagcataaa gggaaagtcg gtaatttcat atcccgagaa
      481 ttttccggga aaaaccaaac ctgacagcag catcagtgcg gcgcagggat acagcagcaa
      541 ctgcactccg ctcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 catcagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccgaccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccatcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtatgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccac
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggccggtt tgcagggcgc gggcaagacg
     1381 acgaccgccg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtcttga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggt gtcgtattga ccaagatgga cggcgattcg
     1801 cgcggcggcg cagctttgtc cgtgcgccac gtaaccggca agccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgtcc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctacaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtgg aagccatcat caactcgatg actccaaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctg
//
LOCUS       DAXIEB010000170         2450 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000170 DAXIEB010000000
VERSION     DAXIEB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.170"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            40..1395
                     /locus_tag="ACFOA3_002001"
     CDS             40..1395
                     /locus_tag="ACFOA3_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6898939.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIHNQYALILSYTLIGIAWAGIITYPLTIVTNALSGEHMGT
                     YLGLFNGSICMPQIVASLLSFILFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1399..2418
                     /locus_tag="ACFOA3_002002"
     CDS             1399..2418
                     /locus_tag="ACFOA3_002002"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6898940.1"
                     /translation="MSDTPTTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDIREATIAYG
                     IESLS"
BASE COUNT          453 a          670 c          729 g          598 t
ORIGIN      
        1 atctactata cttttcaaat caaagaaagg atttacctta tgtcggaata tacgcctcaa
       61 acagcaaaac aaggtttgcc cgcgctggca aaaagcacga tttggatgct cagtttcggc
      121 tttctcggcg ttcagacggc ctttaccctg caaagctcgc aaatgagccg catttttcaa
      181 acgctaggcg cagacccgca cagcctcggc tggttcttta tcctgccgcc gctggcgggg
      241 atgctggtgc agccgattgt cggccattac tccgaccgca cttggaagcc gcgtttgggc
      301 ggccgccgtc tgccgtatct gctttatggc acgttgattg cggttattgt gatgattttg
      361 atgccgaact cgggcagctt cggtttcggc tatgcgtcgc tggcggcttt gtcgttcggc
      421 gcgctgatga ttgcgctgtt agacgtgtcg tcaaatatgg cgatgcagcc gtttaagatg
      481 atggtcggcg acatggtcaa cgaggagcag aaaggctacg cctacgggat tcaaagtttc
      541 ttagcgaata cgggcgcggt cgtggcggcg attctgccgt ttgtgtttgc gtatatcggt
      601 ttggcgaaca ccgccgagaa aggcgttgtg ccgcagaccg tggtcgtggc gttttatgtg
      661 ggtgcggcgt tgctggtgat taccagcgcg ttcacgattt tcaaagtgaa ggaatacgat
      721 ccggaaacct acgcccgtta ccacggcatc gatgtcgccg cgaatcagga aaaagccaac
      781 tggatcgaac tcttgaaaac cgcgcctaag gcgttttgga cggttacttt ggtgcaattc
      841 ttctgctggt tcgccttcca atatatgtgg acttactcgg caggcgcgat tgcggaaaac
      901 gtctggcaca ccaccgatgc gtcttccgta ggttatcagg aggcgggtaa ctggtacggc
      961 gttttggcgg cggtgcagtc ggttgcggcg gtgatttgtt cgtttgtatt ggcgaaagtg
     1021 ccgaataaat accataaggc gggttatttc ggctgtttgg ctttgggcgc gctcggcttt
     1081 ttctccgttt tcttcatcca caatcaatac gcattaatcc tgtcttatac cttaatcggc
     1141 atcgcgtggg caggcattat tacctatcct ttgacgattg taaccaatgc cctctccggc
     1201 gaacacatgg gtacttattt gggcctgttt aacggctcta tctgtatgcc gcaaatcgtc
     1261 gcttcgctgt tgagcttcat attgttcccg atgctgggcg gcttgcaggc cactatgttc
     1321 ttggtagggg gcgtcgtcct gctgctgggc gcgttttccg tgttcctgat taaagaaaca
     1381 cacggcgggg tttgagcgat gagcgatacc cccactaccc ccgctacccg cgatttcggc
     1441 ctgatcgacg ggcgtgccgt aaccggctat gtgctgtcca atcggcgtgg tacgcgcgtc
     1501 tgcgtgctgg acttgggcgg gattgtgcag gaattttccg ttttggcaga cggcgtgcgc
     1561 gaaaacctcg tggtgtcgtt cgacgatgcg gcttcctatg cggacaatcc gtttcagatt
     1621 aacaaacaga tagggcgcgt ggccggacgc atccgcggtg cggcgttcga catcaacggc
     1681 aggacttacc gcgtggaggc caacgaaggc aggaacgcgc tgcacggcgg ttcgcacggg
     1741 ctggccgtta cccgtttcaa cgcggtggcg gcagacggcc gttcggtggt gctgcgcagc
     1801 cgcctgcaac agtcggccga cggttatccc aacgatttgg atttggatat ttcctaccgc
     1861 ttggacgagg acgaccggct taccgttagc tatcgcgcca ccgcgctcgg cgacacggtg
     1921 ttcgacccga cgctgcacat ttactggcgg ctggacgcgg gcctgcacga tgcggttctg
     1981 catattccgc agggcggaca tattccggcc gatgccgaaa aactgcccgt ctcaacggtt
     2041 tcagacgacc tcgaagtatt tgatttcagc cggcccaagc cgctggatgc cgccgttgcc
     2101 gccctgcgcc gcgaaacggg tcgggccggt tttgacgacg cttaccgcgt gccgtccgat
     2161 ataggccgtc ccgccgctgt gttgcaagcc ggacgccgcc gtcgtatcag catatacagc
     2221 gaccgcaacg gcttggtcat ctttaccgcc gccccgcagg atttcgcgcg gcacgatgcg
     2281 ggcgtttacg acgcgctggc gaccgaggcg cagacgctgc ccgacagcct gcattggccc
     2341 gagttcggca atattcgtct gaacaagggt gatatcaggg aggcgacgat tgcttacggc
     2401 atcgaatccc tttcttagga gcttcctaac accggttgca gacgaccttt
//
LOCUS       DAXIEB010000171         2450 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000171 DAXIEB010000000
VERSION     DAXIEB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.171"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..336
                     /locus_tag="ACFOA3_002003"
     CDS             <1..336
                     /locus_tag="ACFOA3_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6898941.1"
                     /translation="LYIIATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDH
                     AQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKAC
                     CGKYLKPVA"
     gene            complement(553..1347)
                     /locus_tag="ACFOA3_002004"
     CDS             complement(553..1347)
                     /locus_tag="ACFOA3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6898942.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1556..2281
                     /locus_tag="ACFOA3_002005"
     CDS             1556..2281
                     /locus_tag="ACFOA3_002005"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6898943.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFDAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          659 a          768 c          547 g          476 t
ORIGIN      
        1 ctatacatca tcgctactac cgttccggcg caacaaacat tcctcgatgc cgccgaactg
       61 atgcaatgga gtatagaaac cgaagggctg ggcttgaacg tcatctcgca caagatactc
      121 ggcaaagacc acgcccaagt cgaatttgaa gcctacttcc gagacggaca acaccgatcc
      181 gcgcatcacg aactgtccgg cttcgtcaac atcggcggac aatggtattt tatcgatcct
      241 accgttccgc atcctgcgat gaaacaaccc tgcatttgcg gatcgggcaa aaaattcaaa
      301 gcctgctgcg gcaaatatct gaaacctgtc gcataaaaat gccgtctgaa cgttcagacg
      361 gcattttcaa cacgcaaaaa aaccattcat accaagggta agtatgaatg gtcaatacat
      421 tgcgggaaaa cgtcttactt gctgcactgc cgaaaaggga gaaacggcag cggtaatcag
      481 cggaaaggat tgtacccgaa ttaatgttaa gaaacgttaa tcgtgaaaat atattaacaa
      541 acctgttgaa acctattggt tttcccgtat ccactcgacc cagcgttcaa acagcttcgg
      601 ttcgagcgcg gcgacgaccg agcgtttgaa cacgtgttca ccactccaaa acccgtcgcc
      661 ttccaaagtc gtcagcctgc cgcccgcctc ctcgaaaatc aacgcgccgg cggcataatc
      721 ccacagcttc tgcccgccgt ggacataaac atcataacgc ccgcacgcca gataacacca
      781 gtccaacgta ctgctgccca tactccgtat cgttccaaaa ggcgcgagcg tactcatgcg
      841 gctggacagt ttgcccgaac gcaaatattt gatttccacg cccgcaatcg cctcattgag
      901 ttttttatcc acgagacgca aaggcaggcg ggttccatta agaaacgcac cctgcccccg
      961 ttcggcataa aaacattcgc cgctgaccgg gttgtagatt acgcccaatt ccgcacgccc
     1021 gttgcggaca aacgccaccg acaccgcaaa atgcggcagc ccgttgacaa aattgttcgt
     1081 cccgtcgatc ggatcgacaa tccacagccc cttttccccc gaatattgtt cccacaaagc
     1141 cgactgttcc tgccgcgaca tttcctcgcc caacatcggg caatcgatca gaagcggcaa
     1201 cgcggcggca aatgccgtct gcgcggcaat gtccgcctcg ctcaacatcg aaccgtcttc
     1261 cttgcggcaa gacggcgtat tcaaaaaacg cggcataatt tcggtttgcg cgatatggcg
     1321 cacgactttc tgcaaacggt gtaacacttc ctactgtcct catattttga acttgcggcg
     1381 cgcgaacgta tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
     1441 acaaatagta cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactataatg
     1501 tccgtttcca tcacgccgct gcgacagatt ataaccgtcc gaaccgccaa aaactatgcc
     1561 ccgattccac ctgcccgaaa acctttccgt cggacaaacc gtcgccttgc ccgacaacat
     1621 cgtccgccac ctcaacgtcc tgcgcgtccg ccccaacgaa aacatcaccc tcttcgacgg
     1681 caaaggcaag gcacacgccg cacggctgac cgttttggaa aaacgccgcg ccgaagccga
     1741 aatcctgcac gaagacacaa ccgacaacga gtccccgctc aacatcacac tgatacaatc
     1801 catctcctcc ggcgaacgta tggactttac cctgcaaaaa agcgtcgaac tcggcgtaac
     1861 cgccatacag cccgtcatca gcgaacgctg catcgtccgc ctcgacgggg aacgcgccgc
     1921 caaacgcctc gcacgctggc aggaaatcgt catctccgcg tgcgaacaaa gcggcaggaa
     1981 caccgttccc cccgtactgc ccatcatcgg ctaccgtgaa gcactcgaca aaatgccgtc
     2041 tgaaagcacc aagctgatta tgagcatcaa ccgcgcccgc aaactcggcg acatccgcca
     2101 accgtccggc gcaatcgtct ttatggtcgg gcctgaaggc ggctggacag aacaggaaga
     2161 acaacaggca tttgatgccg gctttcaggc ggttacactc ggcaaacgga ttttacgcac
     2221 agaaaccgcc ccactcgccg ccctcgccgc catgcagacg ctttggggcg atttcgcata
     2281 aacagaaatg ccgtctgaaa cccgttcaga cggcattttg cagccgatta agatagtagg
     2341 ttcaaataag atttcccgtg tcgtcattcc cgcgaaagcg ggaatctaga aacgaaaaac
     2401 tacagggatt tatccgaaac aacaaccctc tccgccgtca ttcccgcgca
//
LOCUS       DAXIEB010000172         2423 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000172 DAXIEB010000000
VERSION     DAXIEB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.172"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(56..565)
                     /locus_tag="ACFOA3_002006"
     CDS             complement(56..565)
                     /locus_tag="ACFOA3_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6898944.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(896..1228)
                     /locus_tag="ACFOA3_002007"
     CDS             complement(896..1228)
                     /locus_tag="ACFOA3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898945.1"
                     /translation="MQILSFQPDIAERMLEGTESESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            1567..2403
                     /gene="cysT"
                     /locus_tag="ACFOA3_002008"
     CDS             1567..2403
                     /gene="cysT"
                     /locus_tag="ACFOA3_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6898946.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT          588 a          585 c          588 g          662 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccgctat aattgatgaa acgggttaaa
       61 aaagtgttgc catcgcctgt tcttcttgtc ggatacgctt aaataagacc agcaaataaa
      121 tgggcagacc aatcaataaa acataccacg cttgacataa taaggcgatg ccaatcagtt
      181 cgggtatgat gtttaaaaaa taattggggt ggcggaatgt tttaaacaac cacgaacgat
      241 taatttgatg atttggtaaa atatagattt taaccgtcca aatctccccc aactgcttaa
      301 taatcaatga caatatcaca aatgaagcca tcaccgtcag cgtaccaatc aaggatatgc
      361 cattaaaagc agtgtcagaa agccataccc aaacaaaaca cgccaaataa taaagcgtat
      421 gaactgccgc aagcagcgtg gaattggttt ttccgtattg tttcgcccct ttggcaatca
      481 aggctttttc atgtttaata gagacggcta aaaataacag tctgatgata aaaaacaggc
      541 ttaaaatgct taaaatcatt gtcattgatg tttttccatt gaaattgaaa taaatataaa
      601 tcggattaat ggtattttta attaatgatg tttcagacca tcatgctcta taaacaattc
      661 ccattaagtc cgcgccgcaa cctgctataa taagtctgca atcggcgcaa atcaatgctt
      721 tgcgtttatt gccatcccaa aataattgat gatgccttaa ttataatacc aagataagtt
      781 ttttattcaa taaaatacaa agggaagcgt tcagcccatt gcaaacagat gcaatccacc
      841 gattatttaa aaaacggcaa agccttgccc ctcttgcggc aagcctgcaa tgcctttaat
      901 gtccgcaggc gcaagcgtcg ccgtggtgcc cgcaaccctg tgccgtttcg agccattcgt
      961 cgtcatcgcc gtcaaactct tccacttcgt caaactcgcc gcgttccacc atgacgcaca
     1021 actcttcaag gctttccgcg ccttccaccc aaaaacaggg aatgcccggc ggcgtatcgg
     1081 gcgcaagcag tgccgctacg ccttcatgcc acgcaatcca atagccgttg tccgtacgga
     1141 cgaattgtgc gttttcgttg accgactcgc tttccgtacc ttccagcata cgttccgcaa
     1201 tgtccggctg aaaagataaa atctgcataa gtgttccttt atatgatggt tttccgtcaa
     1261 aacaaggcgt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     1321 gagaacgatt ctctaaggtg ctaaagcacc aagtgaatca gttccgtact atttgtactg
     1381 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatttta aaacatcgcg
     1441 cccgcttgag aaactgccaa ccgctttata acaaattcgt ctttgcacca aactttccat
     1501 tctttccgtt tttcggacgg catcgttaaa gtagtccttc cttttcctta ttttcagcat
     1561 tgttttatgt taccgctcaa aacgcccggc gtactgcccg gcttcaaact cagccttggt
     1621 ctgaccgtat tgtgcctgtc gctgcttgtg gtcttgccgt ttgcgatgat ggcggcgaag
     1681 gcggcggaaa tcggctgggg cggcttttgg aacacgattg ccgagccgaa cgtgttggcg
     1741 gcggtgtggc tgagtttgcg gatgtcgttt tatgcgatgc tgaccaatgt cgtgttcggc
     1801 acgctggtgg cgtgggtatt ggtgcgttat gaattcccgg gcaagggtct ggcgaacgcg
     1861 ctggtcgatt tgccgtttgc gctgccgacg gcggttacgg gtatcgcgtt ggcaaccctg
     1921 tatgcgccca acggttggat aggccgtttt ttcgagcctt tgggcatcaa aatcgcgttt
     1981 acgcccgtcg gcatttggat tgcgctggtc gtcgtcagcc tgccctttat cgtccgcgcc
     2041 gtgcagccgg tgttggagga attatcgggc gaatatgagg aagcggcggc gactttgggc
     2101 gcaaaccgct ttacgacgtt ccgccgcgtg ctgctgccgg aaatcacgcc ggcactcttg
     2161 accggcgcgg gaatgatgtt cgcgcgggca acgggggaat acggctcggt gatttttatc
     2221 gcgggcaaca ttccgatggt ttccgaaatc ctaccgctga ttattacggg caagctggaa
     2281 cagttcgacg tgcagggcgc gtcggcggtg gcgttgttta tgctgctggt ttcgtttgtc
     2341 atcctgtttg cgctgaacgt gatgcagtgg gcgttgggca ggcgttcggg cgcgaagggt
     2401 tgaggtcgtc tgaaatacct gtt
//
LOCUS       DAXIEB010000173         2365 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000173 DAXIEB010000000
VERSION     DAXIEB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.173"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..2161
                     /gene="pilC"
                     /locus_tag="ACFOA3_002009"
     CDS             <1..2161
                     /gene="pilC"
                     /locus_tag="ACFOA3_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6898947.1"
                     /translation="KGSVSVKLKLPEVKAGRCINKDNPNPKSKAPSPALTAPALWFGP
                     VQNGKVQMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPGRHSLEPLNDTQIKSKEPSF
                     TGRQTIIRLDGGVQQIKLQRNEVANFNSDNGTFGIVKEGSVVPESNEWKKVLLPWTVR
                     GVNDDQFKTFNQQSDKYSQRYRIRDNNKGERNLGDIVNSPIVAVGEYLATSANDGMVH
                     IFKKNGGGDDRNYSLKLSYIPGTMPRKDIESKDSTLAKELRAFAEKGYVGDRYGVDGG
                     FVLRQVELSGQKHVFMFGAMGFGGRGAYALDLTKADGNDPTKASLFDVKDNGNNGNNG
                     NNRVELGYTVGTPQIGKTHNGKYAAFLASGYATKTIDDQTNKTALYVYDLESSGTLIK
                     KIEVPNSKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYRFDLSGNNPNSWTVRTIFEG
                     NKPITSAPAISQLKDKRVVIFGTGSDLSEEDVDSKEIQHVYGIFDNDTDTGTAQDGQG
                     NGLLEQVLKKDGNTLFLSDYKRSDGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIHK
                     YTGNDKCGAETAILGINTADGGKLTKKSARPIVPDTNTKVAQYSGHKKTSSGKSIPIG
                     CMEKDNGIACPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRAGKNNR
                     CFSQKGVRTLLMNDLDSLDITGPMCGMKRISWREVFF"
BASE COUNT          679 a          607 c          614 g          465 t
ORIGIN      
        1 gaaaggatca gtcagcgtca aactcaaatt gccggaagtc aaagcaggcc gctgcatcaa
       61 caaagataac cccaatccca aatccaaagc cccttcgccc gcactgactg cccccgcgct
      121 gtggttcgga cctgtgcaaa atggcaaggt gcagatgtat tccgcttcgg tttccaccta
      181 ccccgacagt tcgagcagcc gcatcttcct tcaaaatctg aaaagaaaaa acgaccccaa
      241 caaacccggt cgccattccc tcgaaccctt gaatgatacg cagattaaaa gtaaagagcc
      301 gagtttcacg gggcggcaaa caatcatccg attggatggc ggcgtacagc agattaaact
      361 tcaaagaaat gaggtcgcca attttaatag cgacaacgga actttcggca ttgttaagga
      421 agggagcgtc gttcctgaaa gcaacgagtg gaaaaaagtg ctgctgcctt ggacggttcg
      481 gggtgtcaat gacgatcaat ttaaaacatt caaccaacaa tcagacaaat acagccaaag
      541 ataccgcatc cgcgacaaca acaagggcga acgcaatttg ggcgacatcg tcaacagccc
      601 catcgtggcg gtcggcgaat atctagctac ttccgccaac gacgggatgg tgcatatctt
      661 caaaaaaaac ggcggcgggg atgaccgcaa ctatagtctg aagctcagct acatccccgg
      721 cacgatgccg cgcaaggata ttgaaagcaa agactccacc cttgccaaag agctgcgcgc
      781 ctttgccgaa aaaggctatg tgggcgaccg ctacggcgtg gacggcggct ttgtcttgcg
      841 ccaagtcgaa ctgagcgggc aaaaacacgt gtttatgttc ggcgcgatgg gctttggcgg
      901 cagaggtgcg tatgccttgg atttgaccaa agccgacggc aatgacccga caaaagcctc
      961 tttgtttgat gtaaaagata acggcaataa tggcaataac ggcaataatc gcgtggaatt
     1021 aggctacacc gtcggcacgc cgcaaatcgg caaaacccac aacggcaaat acgccgcttt
     1081 cctcgcctct ggttatgcga ctaaaacaat tgacgaccaa acaaataaaa ccgcgctgta
     1141 tgtgtatgat ttggaaagca gcggcacgct gattaaaaaa atcgaagtac ccaacagcaa
     1201 gggcgggctt tcgtccccca cgctggtgga taaagatttg gacggcacgg tcgatatcgc
     1261 ctatgccggc gatcgcggcg ggaatatgta ccgctttgat ttaagcggca acaacccgaa
     1321 cagttggact gtacgcacta tttttgaagg caacaaaccg attacttccg cgcccgccat
     1381 ttcccaactg aaagacaaac gcgtggttat cttcggcacg ggcagtgatt tgagtgaaga
     1441 ggatgtggac agcaaagaaa tccaacacgt ttacggtatt tttgacaatg acacagacac
     1501 gggtacggcg caagacgggc agggcaacgg gctgctcgag caagtgctga aaaaagacgg
     1561 caatacgctg ttcttaagcg actacaaaag gtctgacggc tcgggcgaca agggctgggt
     1621 agtgaaattg gaagccggac agcgcgttac cgtcaaaccg accgtggtat tgcgtaccgc
     1681 ctttgtaacc atccataaat atacgggtaa tgacaaatgc ggcgcggaaa ccgccatttt
     1741 gggcatcaat accgccgacg gcggcaagct gaccaagaaa agcgcgcgcc cgattgtgcc
     1801 ggacaccaat acgaaggtcg cgcaatattc cggccataag aaaacttcca gcggcaaatc
     1861 catccctata ggttgtatgg aaaaagacaa tggaatcgcc tgcccgaacg gatatgttta
     1921 cgacaaaccg gttaatgtgc gttatctgga tgaaaagaaa acagacggat tttcaacaac
     1981 ggcagacggt gatgcgggcg gcagcggtat agaccccgac ggcaagcgtg ccggcaaaaa
     2041 caaccgctgc ttctcccaaa aaggggtgcg caccctgctg atgaacgatt tggacagctt
     2101 ggacattacc ggcccgatgt gcggtatgaa acgaatcagc tggcgtgaag tcttcttctg
     2161 atttgcacgc gaaaatgccg tccgaaaggt tttcggacgg cattttttgc gtttttcggg
     2221 aggggcgggt tcgtaaaagg cgggctatag ggtaggcttc atctcgccaa tctcactgaa
     2281 tccatcaatt tccacaattc aattaaatac cgtcaaaccg atgccgtcat tcccgcgcag
     2341 gcgggaatct agaccttaga acaac
//
LOCUS       DAXIEB010000174         2334 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000174 DAXIEB010000000
VERSION     DAXIEB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.174"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..302
                     /locus_tag="ACFOA3_002010"
     CDS             <1..302
                     /locus_tag="ACFOA3_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6898948.1"
                     /translation="VLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNS
                     DWNCGDNIWLIQWFAPFGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            357..2144
                     /locus_tag="ACFOA3_002011"
     CDS             357..2144
                     /locus_tag="ACFOA3_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6898949.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPGYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTLLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
BASE COUNT          655 a          515 c          559 g          605 t
ORIGIN      
        1 ctgtgttaaa aaacgggcag ttcgctttgt tttgcaaggg tacccaacca atcggttata
       61 tctcatgggc ttattttgat gaagtggcgc aggcgcatta tttagaatct gaccgccatt
      121 tgcgtgacaa cagcgattgg aactgtggcg acaatatttg gctgattcaa tggtttgcgc
      181 catttggaca cagtcatcaa atgcgctcag ctgtgcgcca gttatttcct agtacgacag
      241 tacgcgcctt gtatcataaa gggagcgata agggtttgag aattttaact tttaaaactt
      301 gatgcaatcg tgtattgaat gcccattgat gattttattg attttgcgac catgccatga
      361 aacttccttt atcctatttg cctaatattc gctttttgtc ttggtgctgc ttattggcag
      421 gtatcattgc tcctgctact ttgttggcct cccccaaccc tgccgaaatc cgtatgcagc
      481 aagatattca gcaacgccaa cgcgaagagc agttgcgcca aaccatgcag cctgaaagcg
      541 atgtgcgttt gcatcaaaaa aacacgggag aaacggttaa tcagttgatg ggcgatgaca
      601 gcagccaacc gtgttttgcc attaacgaag tggtgttgga aggcgaacac catgctcggt
      661 ttcagtttgc cctaaaacgt gccttgcgcg aaacgggttt tcaggctggc aagtgtctgc
      721 atgcgggcaa cattaatcaa atcatgtcct tagcacaaaa tgctttgatc ggcaggggat
      781 ataccacgac ccgtatcttg gctgcgccac aggatttgaa tagtggcaag cttcaattaa
      841 ccctgatacc gggctatctg cgctccatac gaatcgatcg gtctaacgat gatcaaaccc
      901 atgcaggacg tattgcagca ttccagaaca aatttcccac ccgctcgaac gatctgttga
      961 atctgcgtga tttggaacaa ggactggaaa atctcaaacg tctcccgact gcggaagccg
     1021 atctccaaat cgttcccgta gagggagaac caaaccaaag tgatgtcgtg gtgcaatggc
     1081 ggcaacgtct gctgccctac cgtgtgagtg tggggatgga taattcgggt agtgaggcga
     1141 caggaaaata ccaaggaaat atcactttct ctgccgacaa tcctttggga ctgagtgata
     1201 tgttctatgt aaattatgga cgttcaattg gcggtacgcc cgatgaggaa agttttgacg
     1261 gccatcgcaa agaaggcgga tcaaacaatt acgccgtaca ttattcagcc cctttcggta
     1321 aatggacatg ggcattcaat cacaatggct accgttacca tcaggcagtt tccggattat
     1381 cggaagtcta tgactataat ggaaaaagtt acaatactga tttcggcttc aaccgcctgt
     1441 tgtatcgtga tgccaaacgc aaaacctatc tcggtgtaaa actgtggatg agggaaacaa
     1501 aaagttacat tgatgatgcc gaactgactg tacaacggcg taaaactgcg ggttggttgg
     1561 cagaactttc ccacaaagaa tatatcggtc gcagtacggc agattttaag ttgaaatata
     1621 aacgcggcac cggcatgaaa gatgctctgc gcgcgcctga agaagccttt ggcgaaggca
     1681 cgtcacgtat gaaaatttgg acggcatcgg ctgatgtaaa tactcctttt caaatcggta
     1741 aacagctatt tgcctatgac acatccgttc atgcacaatg gaacaaaacc ctgctaacat
     1801 cgcaagacaa actggctatc ggcggacacc acaccgtacg tggcttcgac ggtgaaatga
     1861 gtttgtctgc cgagcgggga tggtattggc gcaacgattt gagctggcaa tttaaaccag
     1921 gccatcagct ttatcttggg gctgatgtag gacatgtttc aggacaatcc gccaaatggt
     1981 tatcgggcca aactctagtc ggcacagcaa ttgggatacg cgggcagata aagcttggcg
     2041 gcaacctgca ttacgatata tttaccggcc gcgcattgaa aaagcccgaa tttttccaat
     2101 caaggaaatg ggcaagcggt tttcaggtag gctatacgtt ttaaaacggc atagtcaaat
     2161 caacggtaac tatagatata gcttttcata ataaaacacc tatcatgatt atggtaagag
     2221 atggctgtgt tttgatatta gattgacaca ggaatttaag aaggaatcat gaaatacatg
     2281 attccttctc gtttgcctac attaaacata taggccgcca actcaatcaa taga
//
LOCUS       DAXIEB010000175         2198 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000175 DAXIEB010000000
VERSION     DAXIEB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.175"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            31..516
                     /locus_tag="ACFOA3_002012"
     CDS             31..516
                     /locus_tag="ACFOA3_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898950.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            613..1236
                     /gene="rsmG"
                     /locus_tag="ACFOA3_002013"
     CDS             613..1236
                     /gene="rsmG"
                     /locus_tag="ACFOA3_002013"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6898951.1"
                     /translation="MERKERLQSGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1335..2108
                     /locus_tag="ACFOA3_002014"
     CDS             1335..2108
                     /locus_tag="ACFOA3_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6898952.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          424 a          503 c          755 g          516 t
ORIGIN      
        1 tgcggtctgt ttcggaagag ggggacggcg atgtttgtga acgagaaata tccttatgcg
       61 gctctgtttg cggggctggt gtttttgacg ttgccgtttg cgttggcggt gcatgatgcc
      121 tttgcgcttg cgttcggacg ggcagggctg ctggtgtcgg tgtcggccgg cggattcggc
      181 cggcgtggtg gttgggacgg cactgtttgg tttgtgttcg gtgtgtttgc gtttttgaac
      241 ctggtcgtgt cggcaggcct gacgaagctg gcgtacaaaa agatgatgcg gcggcattcg
      301 cgttacgcgc tgtttctgtc gggcgtggcg gcttgcgcgg cggcggcggt ggcttggatt
      361 ttcgagctgt tgttgggcag tgccgccttg ggcgggctgc gcggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgctt gacgcgcgcg
      481 ccagtgcagc cggtagtgtt tcacaggaaa aaataggttg gaacgtgaaa tgccgtctga
      541 aacgccgata cgcggtttca gacggcatgt ttttccgtta acattacgcc tgaacatgga
      601 caggataggg atatggaacg caaagaacgc ctgcaatcgg gcatcgccgc attgggtttg
      661 gatatttccg cagcggcaca ggacaggctt ttggcctatg tggatttgtt gaaaaagtgg
      721 aacaaaacct acaatctgac cgccctgcgc gacgaggaaa aaatgattgt ccatcatctt
      781 ttggacagcc tgacgctgct gccccatatc gagggtgtgc aaacgatgct ggatgtcggt
      841 tcgggcggcg gtcagcccgg cattccggcg gcggtgtgcc gtccggatgt gcaaataacc
      901 cttttggatg cgaatacgaa gaaaacggct tttttacagc aggcggttat cgagttgggg
      961 ttggacaatg tgcgcgtggt atccggacgc gtggaggcgg tttcggacgt gcgtgccgat
     1021 gtggttacca gccgtgcgtt tgcagaactg gcggattttg tgtcgtggac ggtgcatctg
     1081 ttgaaagacg gcggctactg ggcggcgatg aagggcgtgt atccgcagga agaaatcggc
     1141 cgcctgccgc aggatgtgtg cgttgaaaaa gtccaaaggc tcgacgtgcc gggcttggat
     1201 gcggaacgcc atatcgtcat cctgagcaag cgttgagcgt acttcagacg gcatgaatac
     1261 cttttttgtg cggataaagg taaaattccg cactgttttt cttttttcaa catcagacgg
     1321 gacacgggcg ggacatgagt gcgaacatcc ttgccatcgc caatcagaag ggcggtgtgg
     1381 gcaaaacgac gacgacggta aatttggcgg cttcgctggc atcgcgcggc aaacgcgtgc
     1441 tggtggtcga tttggatccg cagggcaatg cgacgacagg cagcggcatc gacaaggcgg
     1501 gtttgcagtc cggcgtttat caggtcttat tgggcgatgc ggacgtgaaa tcggcggcgg
     1561 tacgcagcaa agagggcgga tacgctgtgt tgggtgcgaa ccgcgcgctg gccggcgcgg
     1621 aaatcgagct ggtgcaggaa atcgcccggg aagtgcgttt gaaaaacgcg ctcaaggcag
     1681 tggcggaaga ttacgacttt atcctgatcg actgtccgcc ttcgctgacg ctgttgacgc
     1741 ttaacggctt ggtggcggcg ggcggcgtga ttgtgccgat gttgtgcgaa tattacgcgc
     1801 tggaagggat ttccgatttg attgcgaccg tgcgcaaaat ccgtcaggcg gtcaatcccg
     1861 atttggacat cacgggcatc gtgcgcacga tgtacgacag ccgcagcagg ctggttgccg
     1921 aagtcagcga acagttgcgc agccatttcg gggatttgct ttttgaaacc gccatcccgc
     1981 gcaatatccg ccttgcagaa gcgccgagcc acggtatgcc ggttatggct tatgatgcgc
     2041 aggcaaaggg tgccaaggcg tatcttgcct tggcggacga gctggcggcg agggtgtcgg
     2101 ggaaataggt caatccaaat cgggctgccc gtgcctttat gctgtttggc cgggtgcgtt
     2161 aatatggcgg attaaaataa aaatacttat atcgtcat
//
LOCUS       DAXIEB010000176         2172 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000176 DAXIEB010000000
VERSION     DAXIEB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.176"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFOA3_002015"
     CDS             complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFOA3_002015"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6898953.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGHIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLDAQKQLREQGILNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1600..2166
                     /locus_tag="ACFOA3_002016"
     CDS             1600..2166
                     /locus_tag="ACFOA3_002016"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6898954.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          500 a          533 c          619 g          520 t
ORIGIN      
        1 atttaatcca ctatattttc ctgtttcagg ctcggcgttc tttgcacatc agtttcgcat
       61 ccagccagcc atcgccgccg gagatgatgt tgttggcaac gaagttgtcc aagtcgggca
      121 gcagggaaac ggcgcgtccg ctgttgatga tgacgaccac cgccatcggt ttgtcgccca
      181 gccaataacc tgcaagggcg cggacattgt tgagcgtgcc ggtttttaag cgcaacagcc
      241 cgccgctttg tttgaagcgg ttgcgtaaag ttccgtctgt gccggcgatg ggcagcgtgt
      301 cgatgaaatc ttgtgcaaac gggctgaaat aagccgtttc caacatttgc gccatcattc
      361 tcgccgttac cctttctttt ctggacagac ctgaaccgtt ttccaaaacc aaatccgcaa
      421 tatcgatgcc cgacacggca agttcgcgcc ggacggcaga cgccgcctgt tcggaaacgg
      481 cgggcagttt gccgtcgccg ccgagtttga ggaagacgga acgcgcaatt agattgtccg
      541 aacgcttgtt catgtccgtc aaaatttcct tcatcggttt ggcgtgtgca acggcaagcg
      601 tctgcgcgcc ttccggcgtg tcggctatgc cgataccgtc tgaaatccgt ccgccgccga
      661 gcagccagtg gttggtaaaa ctttgccgga tcagttcgtc aagcgcgaac atccggacac
      721 cgacaggctt gcccaaacag ctctcgggaa tattgccgcg caatttcagc gtattgtccg
      781 aaaaagatgc acgcatcagt tttttgatcg aagggcaggc agcttgggag gcggtaattt
      841 tcaagttgtt ttgggcgaaa atatgcggca aaggcggatc ggtgaggatg tcggtactgc
      901 cggcggcatt gcgttcggcg cgcaccataa ccataccggc agacagcata gttggattgg
      961 ggggcgtcat aaacggcgaa ccgctgtcgg cttcgaaatc gtcggggctg ccgacttcgc
     1021 cccacaggct gtggtcgagc atcaggtgtc ccgtgatatt gagtatgcct tgttcgcgca
     1081 actgtttttg agcatcaagc aggttttcct gattgaaaac ggggtcgccg ctgcccgccc
     1141 aatacaggtt tccgtcaagc gtgccgtcgt ttaccgtacc gttgctttta aactcggtcg
     1201 cccagcggta attgctgccg aaggttttga aggcggcaaa cgcggtaacg agtttcattg
     1261 tggaggcggg gttgacgggg acatccgagc ggtggtcaat gatgactttt ccgctgtcaa
     1321 gctcttggac atatacggcg atttcgtttt gcggaatgtg acccgtatcg agcgcgtgtg
     1381 cggcgaggga ggcgagaagc agcagcaggg aggccgctgt tttggggaaa ttcataggta
     1441 aatctgttac ataaaaaaag caattataag gcaaagccgg ataagtggga acgctgtggc
     1501 ggcgggccgg cttgtttgca ggggaaatca catatataat aatcgctatc attatgaaat
     1561 gattgagacg cacaaactta atattcagga ggaatgatta tggctaagaa aatcagtatt
     1621 ttggtgggca gcctgcgccg agcttctttt gcgcgcaaag tggcattgaa tgcggcggag
     1681 atgttccccg aaggctggca ggcggaaatc gtcgaaatcg gacatctgcc gctttacaat
     1741 ttcgattatg acgaccctgc ggtggaagat gtgccgctac ccgaaagcta cacggctttc
     1801 cgcgaaacga ttaaggcttc ggacggcatt ttgttcgtta cgtccgaaaa taaccgcacc
     1861 attcccgcct gtttgaaaaa tgcggtggac atcggctcga aaccgaatgc cgacgtggcg
     1921 tggaaaaaca aaccggccgg catcatcagc cattccgtcg gcaagatggg cggttacagt
     1981 tcgcaaaaaa acctgcgcct tgcgctgtct tatttcgata tgcccgtaac cggacagccg
     2041 gaagtgtttt tgggcaattc gccgacgctg tttgatgaaa acggcaaatt gatcgactcg
     2101 gcaagggatt ttgttcagtc atatattaat cagtttgtcg gtttgattga aagaaatgcc
     2161 aaataaacaa ac
//
LOCUS       DAXIEB010000177         2058 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000177 DAXIEB010000000
VERSION     DAXIEB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.177"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            124..984
                     /gene="cysW"
                     /locus_tag="ACFOA3_002017"
     CDS             124..984
                     /gene="cysW"
                     /locus_tag="ACFOA3_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6898955.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            981..2054
                     /locus_tag="ACFOA3_002018"
     CDS             981..2054
                     /locus_tag="ACFOA3_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6898956.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
BASE COUNT          511 a          610 c          494 g          443 t
ORIGIN      
        1 gctagacgtt tttattccct taatcaataa aaggttgtct gaaaacgaat ccgccccaca
       61 aaaaacggtt tttcagacgg catccgaaca ccttaaaaca accaaccggg gaacaccacc
      121 gccatgaaac cctattccgc caatcccaac ctgaccgaac cgcgccggct gcgcgtgttg
      181 ctgattgccg ccgcgctggg ctttctgctg ctgatgctgg tcgtgccgct cgtcgccgtg
      241 ttttacgaag ccttaaaagg cggttgggat ttgtacctga aatccttaaa cgatcccgaa
      301 gcgtggtctg ccatcaaatt gacgctgatt actgcgctga ttgtcgtccc cgtcaatgcc
      361 gtcttgggcg tggcgatggc gtggctgctg acccgttttg acttccgagg caagcagttg
      421 ctgaccaccc tgctcgattt gccgttttcc gtatcgccag tggtagccgg tttgatgttc
      481 gtcttattgt tcggcgcgca cacggcattg ggtggccggc tcgaagcgca aggcatacag
      541 attatctttg ccattccggg cattattttg gcgaccttgt tcgttacttt cccctttgtc
      601 gcgcgtgaaa tcattccttt aatgcaggca cagggcgaca gcgaagaaca ggcggcattg
      661 atactcggcg caagcggctg gcagatgttt tggcgcgtta ccctgcccaa catcaaatgg
      721 gcattactct acggcatcat cctcaccaac gcccgcgcga tgggcgagtt cggcgcggtc
      781 agcgtggtat cgggacacat acgcggcgaa accaacaccg tcccgctttt ggtcgaaatc
      841 ttctacaacg aatacaactt caccggcgca ttcgccctct ccggcgtatt ggcacttttg
      901 gcactggcga cgctggcggt gcagaacatc atcaccaaat tacaagacaa aaaactcgcc
      961 gccgccgaaa ggaacgcagt atgagtatca ccatccaaaa cttaaacaaa cacttcggca
     1021 attttcacgc gctgaaaaac atcaacctca acgtccccac cggcaaactc gtttccctgc
     1081 tcggcccgtc cggctgcggc aaaaccacac ttttacgcat tatcgccgga ctggaaaacg
     1141 ccgacggcgg caatatcctg tttgacgggc aagacgtaac cgccaaacat gtgcgcgagc
     1201 gcaaagtcgg cttcgtgttc caacactacg cccttttccg ccatatgaac gtgtttgaca
     1261 acgtcgcttt cggtttgacc gtattgccca agtccgaacg cccgtccaaa gaacaaatct
     1321 gcactaaagt cgaagaattg ctgaaactcg tacaactctc gcatttggca aaatcctatc
     1381 cgcaccaact ctccggcggg caacgccagc gcatcgccct cgcccgtgcg cttgcggtcg
     1441 aaccgaaact cctgcttttg gacgaaccct tcggcgcgtt ggacgccaaa gtacgcaaag
     1501 aattacgcac ctggctgcgc gacatccatc acaacctggg tgtaaccagc attctggtta
     1561 cgcacgacca agaagaagcc ctcgaagttt ccgacgaaat cgtcgtgatg aaccacggca
     1621 aaatcgaaca aaccggcagc gccgaagcta tttaccgcaa acccgaaaat gccttcgtta
     1681 ccgagttcct cggcgaaacc gacgcatttg aaggacgcat cgaaaaaggc ttctggcatt
     1741 ataacggctt cgcgtggaaa ttggacgcac aatacaaatg gcaggaacaa accgccaccg
     1801 gctatatccg cccgcacgaa tggcagatcg ccgccgaaca cgaaacaccg atgatttgtg
     1861 ccgaaatcga aaaagtccac gccgtcgggg cattgacgca tattttggtg aaacacggca
     1921 aacaagacgt gcacatcacg ctggctggca gcgatgccgc gcgttaccca atcgccgaag
     1981 gcaaagaatt gaagctgatt ccgaaacagg tttatgtctt ctctcaaaac gaactgattg
     2041 aatattcgat ttaaccat
//
LOCUS       DAXIEB010000178         2029 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000178 DAXIEB010000000
VERSION     DAXIEB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.178"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..433
                     /locus_tag="ACFOA3_002019"
     CDS             <1..433
                     /locus_tag="ACFOA3_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6898957.1"
                     /translation="HLQQTNRFNVLNRTNLNALKQESGISGKAHNLKGADYVVTGDVT
                     EFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVNTSEIVYSAQGAGEYALSNREII
                     GFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGAWQPNR"
     gene            443..811
                     /locus_tag="ACFOA3_002020"
     CDS             443..811
                     /locus_tag="ACFOA3_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6898958.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            808..1455
                     /locus_tag="ACFOA3_002021"
     CDS             808..1455
                     /locus_tag="ACFOA3_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6898959.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT          525 a          540 c          506 g          458 t
ORIGIN      
        1 gcacctgcaa cagaccaacc gcttcaacgt actgaaccgc accaatttga acgcattaaa
       61 acaggaatcc ggcatttccg gcaaagcgca taacctgaaa ggcgcagatt atgtcgttac
      121 cggcgatgta accgaattcg gacgcagaga tgtcggcgat catcagctct tcggcatttt
      181 gggtcgcggc aaatcgcaaa tcgcctatgc aaaagtggct ctgaatatcg tcaacgtcaa
      241 tacttccgaa atcgtctatt ccgcacaggg cgcgggcgaa tacgcacttt ccaaccgtga
      301 aatcatcggt ttcggcggca cttccggcta cgatgcgact ttgaacggca aagttttaga
      361 cttggcaatc cgcgaagccg tcaacagcct ggttcaggct gttgacaacg gcgcatggca
      421 acccaaccgt taaggagaaa tgatgaatcc gaaaaccttg agccgtttgt cgctgtgtgc
      481 ggcggtcttg gctctgaccg cctgcggcgg caacgggcaa aaatccctgt attattacgg
      541 cggctatccc gataccgtct atgaaggttt gaaaaacgac gacacttcgt tgggcaagca
      601 gaccgaaaag atggaaaaat actttgtgga agccggcaac aaaaaaatga atgccgcccc
      661 gggtgcgcac gcccatctgg gactgctgct ttcccgttcg ggagacaaag agggcgcgtt
      721 ccgccagttt gaagaagaga aaaggctgtt tcccgaatcg ggcgtattta tggacttcct
      781 gatgaaaacc ggtaaaggag gcaagcgatg aaacctttga ttttagggct tgccgccgtg
      841 ttggcgctgt ctgcctgcca agttcaaaaa gcgcccgatt tcgactacac gtcattcaag
      901 gaaagcaaac cggcttcaat tttggtggtt ccgccgctga acgaatcgcc cgatgtcaac
      961 ggaacatggg gtgtactggc ttcgaccgcc gctccgcttt ccgaagccgg ctattacgtc
     1021 ttccccgccg cagtcgtgga ggaaaccttc aaacaaaacg gcttgaccaa tgccgccgat
     1081 attcacgccg tccggccgga aaaactgcat cagattttcg gcaatgatgc ggttttgtac
     1141 attacggtta ccgaatacgg cacttcatat caaattttag acagcgtgac gaccgtatcc
     1201 gccaaagcac ggctggtcga ttcccgcaac ggaaaagagt tgtggtcggg ttcggccagc
     1261 atccgcgaag gcagcaacaa cagcaacagc ggcctgttgg gggctttggt cagcgcagtg
     1321 gtcaatcaga ttgccaacag cctgaccgac cgcggttatc aggtttccaa aaccgccgca
     1381 tacaacctgc tgtcgcccta ttctcacaac ggcatcttga aaggtccgag attcgttgaa
     1441 gagcagccca aataagcata tgccgtctga acctctccga gcttcaggcg gcatatgcga
     1501 atcttgatac ttgcaagccg caccgcccaa aatcgggcag ccggtttcaa tatccgaaca
     1561 aggttcgggt tctgtatttt gcagaacccg aaccttttat tcaaatttaa acttaattcc
     1621 ggcgtggttg tatggtatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     1681 cagtacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
     1741 tttgagctaa ggcgaggcaa cgctgtactg gtttttgtta atccactata aattaacacg
     1801 caatgtaccc tgaccggcaa aacttcccca aacttcatca aaaaatcaac tgtacccgtc
     1861 tttttcagac ggcattgata ccgtaagaaa tttcccatgc agaaatggca aaccgagctt
     1921 tattccacgc cgtcttggct gttgcagacc ttattgacgg tcgccgccgc ttcagcggtg
     1981 attctgtttg tcgtgcgcaa aacgcgcttc gggcgcgcat tcagccata
//
LOCUS       DAXIEB010000179         2020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000179 DAXIEB010000000
VERSION     DAXIEB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.179"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(1..1120)
                     /locus_tag="ACFOA3_002022"
                     /pseudo
     CDS             complement(1..1120)
                     /locus_tag="ACFOA3_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(1208..1570)
                     /locus_tag="ACFOA3_002023"
     CDS             complement(1208..1570)
                     /locus_tag="ACFOA3_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898960.1"
                     /translation="MDVLQPNHSDIIRYSGKDFPINQTLNHIHPARYAVTFENNVDSK
                     LRRHWVAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKERLTSS
                     EPLSDFVLSVLKPYILRP"
     gene            complement(1725..>2020)
                     /locus_tag="ACFOA3_002024"
     CDS             complement(1725..>2020)
                     /locus_tag="ACFOA3_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898961.1"
                     /translation="YDSAENRQHLKEHQLQDGIMRKACRNRPLTEVQTKRNRYLSKTR
                     YVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          481 a          515 c          452 g          572 t
ORIGIN      
        1 cttgtgatga ctcggctcaa tcagttcaaa cagtttggct ttgtccaatt cgggaaaaat
       61 gaatttcaga ccgtttgccg cacgtccgaa ctgttttttt acccattcaa ggtaacggtc
      121 ggctgaaatg accttatctt ccttaaccgc gtgtatgcgc gttgcctttt gggcgaatcg
      181 ttcgcaaatc ggatatgcgc cgccgaaata ttcgcccgga ttctgcaaaa cttcgaaagg
      241 aataacgata tcttttgctt tgaattcaat ttcaaagcgc gtccatgtgc ttgttttatc
      301 gcccaactgc ttgccttttt catagacgcg gacatatttg gacgattcac gggagccgat
      361 accataggtc ttgcctttgg tcattttggc ttcatcgtct tcttcccaat ctgaccccaa
      421 acattcgcct tttggtttga cgtgatgaca ggtaaacata cctttatttc ggtcttcacg
      481 ggcttggttc gggctgtatt cgccgttgaa aaagtctttt gcgatgtcaa cgcgtgtgat
      541 ttttgggcgg attgcattag tcaggaatgc gaaaagtcgt gattcccagc cttcttttgc
      601 gacgccgcaa ccggtgccgg tcagttcgaa aaggatggta ttgttttgtc cgccaaaatg
      661 gacgcgaccg tatagggcgt cttccgaacc catcaaccaa cagcgctcat aaaaacgacc
      721 gcccgaacct ttggactctt tgtagatacc gaaaccgaaa acttcttcgg cgagcatgga
      781 cgctgcgcga ataaaatctt cgtcttccaa aagacttaca cgaacgccgt atttatcgaa
      841 aaaggttttt tcatgaaatg aaaagctaat ttgatcaatg aaagccgaat ctgatacacc
      901 gcgcctaaga ggaacgccta acaggtttcc tttaccgtcc gttatgtacg tttcgtaaca
      961 ttcgaagact tcttgaaccc tgcccgccgt ttcggtttct gtaccccccc ctgttagata
     1021 aggggggaag atttgaagcg gttgccagct tcctgccgtc cgtcggcgcg tccgtcatca
     1081 cgccggcaac cgcctttgtc atcccttgct tatcttccat ggtgcgaatc ctcaaaaacg
     1141 ggcaaaaaaa aaaagccctg ttacttgtag aaagtaaagg gcgttaattt ttgttaatcg
     1201 tcccttctta gggacgcaat atataaggtt ttaaaacgct aagaacaaaa tccgataacg
     1261 gctctgaaga agtaagtctt tctttattgc acaagatagc aaacttccac ggcattctcg
     1321 ccccacccgt gccgtccaag cctttttcaa agacatagat ggttttcttg gcaatcactt
     1381 cgtcagtttg gcggtcgata atccgtatgg tcgcacctgc cacccagtgc ctgcgcagct
     1441 tggagtcgac attgttttcg aacgttaccg cataacgggc ggggtgtata tgattaagtg
     1501 tttgatttat cggaaaatct ttacctgaat accgaataat atcggaatgg ttgggttgca
     1561 ggacatccac atagcggaga cctttgcaaa attcctttcc ctcccgacag ccgaaaccca
     1621 aacacaggtt ttcggctgtt ttcgtttcaa atatccacta attttaccca aatatcccct
     1681 taatcctgcc cggatacccg ataatcaggc atccgggctg ccttttaggc agcagcgggc
     1741 gcacttagcc tgttggccgc tttcaacagg ttcaaacaca tcgccttcag atggctttgc
     1801 gcactcactt taatcagtcc gaaataggcc gcccgcgcat agcggaattt acggtgcagc
     1861 gtaccgaagc tttgttcgac cacataacgg gtcttcgaca aatatcggtt gcgtttggtt
     1921 tgcacttccg tcagcggacg gttgcggcag gctttgcgca taatgccgtc ctgcaactga
     1981 tgttctttca gatgttgccg gttttccgca ctgtcgtagc
//
LOCUS       DAXIEB010000180         2000 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000180 DAXIEB010000000
VERSION     DAXIEB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.180"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..186
                     /locus_tag="ACFOA3_002025"
     CDS             <1..186
                     /locus_tag="ACFOA3_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898962.1"
                     /translation="HVPDSRLRGNDEILSWGNLSENPQPPKTGRMPHHPPPNPDLTIQ
                     TNQKKKQNEQNIPHHLE"
     gene            155..1933
                     /gene="nhhA"
                     /locus_tag="ACFOA3_002026"
     CDS             155..1933
                     /gene="nhhA"
                     /locus_tag="ACFOA3_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HFC6898963.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQASANNEEQEEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKGKDENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          625 a          471 c          508 g          396 t
ORIGIN      
        1 cacgtcccag attcccgcct tcgcgggaat gacgagattt taagttgggg gaatttatca
       61 gaaaaccccc aacccccaaa aaccgggcgg atgccgcacc atccgccccc aaaccccgat
      121 ttaaccattc aaacaaacca aaagaaaaaa caaaatgaac aaaatatacc gcatcatttg
      181 gaatagtgcc ctcaatgcct gggtcgtcgt atccgagctc acacgcaacc acaccaaacg
      241 cgcctccgca accgtggaga ccgccgtatt ggcgacactg ttgtttgcaa cggttcaggc
      301 aagtgctaac aatgaagagc aagaagaaga tttatattta gaccccgtac aacgcactgt
      361 tgccgtgttg atagtcaatt ccgataaaga aggcacggga gaaaaagaaa aagtagaaga
      421 aaattcagat tgggcagtat atttcaacga gaaaggagta ctaacagcca gagaaatcac
      481 cctcaaagcc ggcgacaacc tgaaaatcaa acaaaacggc acaaacttca cctactcgct
      541 gaaaaaagac ctcacagatc tgaccagtgt tggaactgaa aaattatcgt ttagcgcaaa
      601 cggcaataaa gtcaacatca caagcgacac caaaggcttg aattttgcga aagaaacggc
      661 tgggacgaac ggcgacacca cggttcatct gaacggtatc ggttcgactt tgaccgatac
      721 gctgctgaat accggagcga ccacaaacgt aaccaacgac aacgttaccg atgacgagaa
      781 aaaacgtgcg gcaagcgtta aagacgtatt aaacgcaggc tggaatatta aaggcgttaa
      841 acccggtaca acagcttccg ataacgttga tttcgtccgc acttacgaca cagtcgagtt
      901 cttgagcgca gatacgaaaa caacgactgt taatgtggaa agcaaagaca acggcaagaa
      961 aaccgaagtt aaaatcggtg cgaagacttc tgttattaaa gaaaaagacg gtaagttggt
     1021 tactggtaaa ggcaaagacg agaatggttc ttctacagac gaaggcgaag gcttagtgac
     1081 tgcaaaagaa gtgattgatg cagtaaacaa ggctggttgg agaatgaaaa caacaaccgc
     1141 taatggtcaa acaggtcaag ctgacaagtt tgaaaccgtt acatcaggca caaatgtaac
     1201 ctttgctagt ggtaaaggta caactgcgac tgtaagtaaa gatgatcaag gcaacatcac
     1261 tgttaagtat gatgtaaatg tcggcgatgc cctaaacgtc aatcagctgc aaaacagcgg
     1321 ttggaatttg gattccaaag cggttgcagg ttcttcgggc aaagtcatca gcggcaatgt
     1381 ttcgccgagc aagggaaaga tggatgaaac cgtcaacatt aatgccggca acaacattga
     1441 gattacccgc aacggtaaaa atatcgacat cgccacttcg atgaccccgc agttttccag
     1501 cgtttcgctc ggtgcggggg cggatgcgcc cactttaagc gtggatgacg agggcgcgtt
     1561 gaatgtcggc agcaaggatg ccaacaaacc cgtccgcatt accaatgtcg ccccgggcgt
     1621 taaagagggg gatgttacaa acgtcgcaca acttaaaggt gtggcgcaaa acttgaacaa
     1681 ccgcatcgac aatgtggacg gcaacgcgcg tgcgggcatc gcccaagcga ttgcaaccgc
     1741 aggtctggtt caggcgtatc tgcccggcaa gagtatgatg gcgatcggcg gcggcactta
     1801 tcgcggcgaa gccggttatg ccatcggcta ctcaagcatt tccgacggcg gaaattggat
     1861 tatcaaaggc acggcttccg gcaattcgcg cggccatttc ggtgcttccg catctgtcgg
     1921 ttatcagtgg taagggcttt tcgcctgtct gctgtttggg ataggcggaa ggtttgaagg
     1981 gaagggtggc gatttgccgc
//
LOCUS       DAXIEB010000181         1962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000181 DAXIEB010000000
VERSION     DAXIEB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.181"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            174..1475
                     /gene="nuoF"
                     /locus_tag="ACFOA3_002027"
     CDS             174..1475
                     /gene="nuoF"
                     /locus_tag="ACFOA3_002027"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6898964.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            1475..1912
                     /locus_tag="ACFOA3_002028"
     CDS             1475..1912
                     /locus_tag="ACFOA3_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898965.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
BASE COUNT          533 a          424 c          490 g          515 t
ORIGIN      
        1 cagacaagca agtggtcgag atccaacaaa aacgattaaa ggtcgtctga aaatatcgat
       61 ttgataaact agattttatt tcagacgacg ttacaagccg atacacaaag acatcttaag
      121 gtcgtctgaa acagcggccg caaccgatac gaaaacaaac aggcacacca aaaatggcta
      181 tttaccaatc aggcgtgatt tttgaccaag tggataccgc caatcccgat tgctggacat
      241 tggacgaata cgtcaaacgc ggcggctata ccgccctgcg taaaattctg tccgaaaaca
      301 tctcgcaaac cgatgtgatt gacgaagtca aaacctccgg cttgcgcggt cgtggcggtg
      361 cgggcttccc gaccggtttg aaatggagct ttatgccccg ttctttcccg ggcgaaaaat
      421 atgtggtttg caacaccgac gaaggcgaac caggtacgtt taaagaccgc gacatcatca
      481 tgttcaatcc gcatgccctg attgaaggta tgattattgc cggttacgcg atgggcgcga
      541 aagcgggcta caactacatt cacggcgaaa tttttgaagg ctaccaacgc tttgaggccg
      601 ctttggagca ggcgcgtgcc gcaggctttt tgggtaaaaa tattttgggt tcggattttg
      661 aattcgaact cttcgcccac cacggctacg gcgcatacat ctgcggcgaa gaaaccgcat
      721 tgctcgaatc gttggaaggc aaaaaaggcc agccgcgctt taagccgcca ttccctgctt
      781 cgttcggcct gtacggcaaa ccgactacca tcaacaatac tgaaacgttc tcctccgttc
      841 cattcattat ccgtgacggt ggacaggcat ttgccgataa aggtattccg aatgcaggcg
      901 gtaccaaatt attctgtatt tccggccatg tcgagcgtcc gggcaactat gaagtgccat
      961 tgggcacacc gtttgccgaa gtcttgaaaa tggcaggcgg tatgcgcggc ggcaaaaaac
     1021 tcaaggccgt tattcccggc ggttcgtccg cgcctgtatt gcctgccgat atcatgatgc
     1081 agaccaatat ggactatgac tcgatttcta aagcaggctc gatgttgggt tccggcgcga
     1141 ttatcgtcat ggacgaagac gtgtgcatgg tgaaagccct tgagcgtctg agctacttct
     1201 actatgacga gtcttgcggt caatgtacgc cttgtcgaga aggtacgggc tggctctacc
     1261 gcatcgtcca ccgcatcgta gaaggcaaag gcaaaatgga agatttggat ttgctggatt
     1321 ccgtcggcaa tcaaatggca ggccgcacca tctgcgccct tgccgatgcc gccgttttcc
     1381 ccgtccgcag ctttaccaag catttccgtg atgagtttgt gcattacatc gaacacggcg
     1441 gaccgatgaa agagcataag tggggagggt ggtaatggtg gaagctaaaa tttttattct
     1501 atacggtgca gccaacaaag gtaagagtac gacactcaat acgcttttta atcagatttg
     1561 tcggaaattt tctaaatttc tagtcttttt tgaaagacat ggaaacggct tagattttgt
     1621 tgcagtattt gatcatgaag gtcagaggat tggtttttat tcatctggtg ataatgaata
     1681 cgaggttagg agaaatttat acaaacttta ttcgcataat tgtgatttta tttttggcac
     1741 gtcaaggaca cggggtggta gttgcgatgc agtaggatgt tatgcagagt tattgcatgg
     1801 cgatgtaaat ataattaatt ggtgtgaaaa gtttgagcct acagatgaag acaatgagcg
     1861 tgctgttaaa gagttattta agtcatttaa aaatataata aatgagttat agttttagtt
     1921 ggttttatat tggttaaaag caaaatgcta aaaatttaac tt
//
LOCUS       DAXIEB010000182         1910 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000182 DAXIEB010000000
VERSION     DAXIEB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.182"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(195..1367)
                     /gene="porA"
                     /locus_tag="ACFOA3_002029"
     CDS             complement(195..1367)
                     /gene="porA"
                     /locus_tag="ACFOA3_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA"
                     /protein_id="HFC6898966.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNFQLQLTEPP
                     SKSQLQVVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGAT
                     QWGNRESFVGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPV
                     SVRYDSPEFSGFSGSVQFVPAQNSKSAYKPAYWTTVNTGSATTTTFVPAVVGKPGSDV
                     YYAGLNYKNGGFAGNYAFKYARHANVGRDAFELFLLGSGSDQAKGTDPLKNHQVHRLT
                     GGYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIER
                     GKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          493 a          490 c          443 g          484 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcgccattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttg atcactcccg
      601 ctgccgagca agaacaactc aaaagcatca cgtccgacat tggcgtgtct cgcatattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gatccgggct tgccgacaac agccggaacg aaagtagtag tagtagcact accggtattc
      781 acagtagtcc aataagccgg cttataggcg gacttgctgt tttgagccgg aacgaattgg
      841 acgctgccgc tgaaaccgga aaattccgga gaatcgtagc gtacggaaac cggcatatcg
      901 tcgtggcgtt tgaaaatacc caattgcgaa gccacatcat tgttgctgtc ccaaggatca
      961 atggcttggc tggcatcgtc aaactgattc gcaacgcgac cggcgcgcag cgtaccgaat
     1021 tcgcctgcca agccgacaaa ggattccctg ttgccccact gggtcgcgcc gccgccggca
     1081 acggatacgt cttgctcaag ctgccaaaca gccttcagcc cttcgcccaa atcctcactc
     1141 cccttaaagc cgataaacga gccgaaatca ctgattttcg tcctgatgcg gcttttggcc
     1201 ttagtaactt ttactacctg aagttgactc tttgagggcg gttcggtcaa ctgcagctgg
     1261 aagttcctgc cttccacgcc ggctttgatt tcgccgtaca ggctgacatc ggcaacggcc
     1321 gcaagcggca gtgcggacaa tacgagggcg gtaagttttt ttcgcatatc ggcttccttt
     1381 tgtaaatttg ataaaaacct aaaaacatcg ggcaaacacc cgatacgtct tcaattatac
     1441 cccccccccc gcaaaaaacc atttttcaga acaaatatct gataaatgcc gcaaccttta
     1501 ttttaaaaat gattatattt tgatataaaa caatagctta ttttttcaaa aacgttgtgt
     1561 ttctacaaca caattcaagc gcagacctcg tgcgggccga tgcgctgctg cccggatgca
     1621 gtcccggctt tttaaaacgc cataaaaaaa cacacgcggc actttatagt ggattaacaa
     1681 aaaccagtac ggcgttgtct cgccttagct caaagagaac gattctctaa ggtgctgaag
     1741 caccaagtga atcggttccg tgctatttgt actgtcttcg gcttcgccgc cttgtcctga
     1801 tttttgttaa tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg
     1861 ggaatctaga ccttagaaca acagcaatat tcaaagatta tctgaaagtc
//
LOCUS       DAXIEB010000183         1874 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000183 DAXIEB010000000
VERSION     DAXIEB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.183"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..216
                     /locus_tag="ACFOA3_002030"
     CDS             <1..216
                     /locus_tag="ACFOA3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6898967.1"
                     /translation="MKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKW
                     RGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            243..662
                     /gene="mafI"
                     /locus_tag="ACFOA3_002031"
     CDS             243..662
                     /gene="mafI"
                     /locus_tag="ACFOA3_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6898968.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            752..823
                     /locus_tag="ACFOA3_002032"
                     /pseudo
     CDS             752..823
                     /locus_tag="ACFOA3_002032"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            820..1062
                     /gene="imm31"
                     /locus_tag="ACFOA3_002033"
     CDS             820..1062
                     /gene="imm31"
                     /locus_tag="ACFOA3_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6898969.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            1140..1385
                     /locus_tag="ACFOA3_002034"
     CDS             1140..1385
                     /locus_tag="ACFOA3_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6898970.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            1388..1777
                     /locus_tag="ACFOA3_002035"
     CDS             1388..1777
                     /locus_tag="ACFOA3_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898971.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADLDEEIDNIYNIIGFVSSYSLSKLRRNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
BASE COUNT          662 a          260 c          377 g          575 t
ORIGIN      
        1 atgaaaaaag gaaggggcgg aaaacctatg acaaatcatt ctatgttccc aaaagattgg
       61 gatgaggcta gaattagggc tgaagttact tcggcttggg agaaaagaaa ggaattagaa
      121 ggtcaaaaat ggagaggtac aagtaaatct ggtgttgaaa tagaaggatt tacggaacct
      181 aatcgaacag cttatccact ttataatagt aaatgaagta ttgattaaaa aggtgttttt
      241 aaatgtataa aataaaatgt ttttatgatc agtttagttc tggtgaatta tttaactact
      301 gccagttttt agataattct tctaatcaaa agataaatac acgaggaacg gtgtatcaat
      361 atattattta tgttttaaca ggagaccttt atctacaaaa agacatagat gaaaatttag
      421 aatttatcca tcaagcagaa aataatccca atgaagttta ttcaggaggt ggacaaggtt
      481 tttgctggga tatttctgct gaaaaagtag ttttctatca caacgaattt gatgaagaag
      541 acggctggcc tgatttaagc tgttcgctgc atacttttaa aactgcttta attgcttgga
      601 atgctttttt gcaattgcct aaaagtattc attcggtggt ggagactgtg attgaggaat
      661 aagcataatt agcttaatga atagaatcag cgatatagat tggactgcaa atccacgctt
      721 atacgctgtg ccatgattaa caggaagaga gcaacttgga tgggctagta gggatggtaa
      781 gcatttaaat atatcaattg atggaaagat tacacacaaa tgactaattt aaaattagat
      841 ttctactctg aagttattat aaaagattct tgccctaatg atttgttaga aaatggtgag
      901 actattaaag gaaaaaaagg agtggtatta ggtataagcg aagaagatgg tataatctat
      961 ggctatacta ttttactttt tgatataaaa tattgtatat atatagataa gaaatatatt
     1021 ataccgactg gaaaaaaatt ctctcgcgat gatttttatt agctaattta agtaatttaa
     1081 gcaagttttt tagatactga aactggtaat tatgtttatg taagcataag gagttcagat
     1141 tgaaggattc tacagggcaa atgaggctgg caaccaagga tttggcggaa gccattaaac
     1201 gaggagaagt acgtagttct gcttttacaa caaagcaact aaaggcaatc gaaaaaggag
     1261 aatctaaaat accggattac acttggcatc atcatcaaga cactggaagg atgcaattga
     1321 ttaatgaaga cttgcatcat gataccggcc atatcggttg gagagcgatg agtaaaggaa
     1381 agtaactatg tggaaaatca taaaagagga tagtgatgat ttaggatttg caattaaatg
     1441 cttattctct caatctattg atttaaatga attcaaatta tggattgaac aagtaatacg
     1501 cgatatgccc attgaggaca tcccttttta tatttttgat ttggcggatt tggatgaaga
     1561 aattgataat atttacaata tcataggttt tgtttcgagt tacagcttat caaaattgag
     1621 aagaaatgcc ttaacaggca ttgccttctt aagggggata gatgtctatg accctcccgt
     1681 ttcaaaagaa aaagcattaa aagccttaga gaaatatcct gaaatttatc agaaatttca
     1741 gcatttcttt ccgtttgtag agctgccgcc gctttaaaca gtcaaaatgc cgtctgaaag
     1801 ccatttccgc cgctcagacg gcattttcgc cccttttgtt tacaaaccct taaaatccct
     1861 ttacactcaa aatc
//
LOCUS       DAXIEB010000184         1838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000184 DAXIEB010000000
VERSION     DAXIEB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.184"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..215
                     /locus_tag="ACFOA3_002036"
     CDS             <1..215
                     /locus_tag="ACFOA3_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6898972.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            250..1761
                     /gene="ubiB"
                     /locus_tag="ACFOA3_002037"
     CDS             250..1761
                     /gene="ubiB"
                     /locus_tag="ACFOA3_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6898973.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFADGRRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
BASE COUNT          409 a          495 c          505 g          429 t
ORIGIN      
        1 atatggagtt agaaacggtc gtgccgccgg aggaaggtga gggcgaagga gagggcgcgg
       61 atggggaaat gcccgaatat cttgaacttc atggcgggcg ggaagatgat atttccgcac
      121 cttcgcaacc cgaaccgccg acccgcaata tcaaaccgct gcttttcggg ctgctgattg
      181 cctttttggg ctatctgctc ggtaagattt tttgattgtc cgataaatgc tgtattcggg
      241 attttatata tgaaatggtt gaaacgcctg acggtcattg tcgggacttt ttaccgctat
      301 cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt ggatatgcgc tctgctgaaa
      361 atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg ccgtccgtct gcgccttgcc
      421 ttggaaagcc tggggccgat tttcatcaag ttcgggcagg ttttgtccac acgccccgat
      481 ttgattccgc acgattacgc cgtcgaactg gcaaagttgc aagacaaagt gccgcctttt
      541 gacgcgcggc tttcgcgtga acaaatcgaa aaatcgctgg gccagcccat cgaaaagctg
      601 tatgcggaat ttgaaaccga gcccgtcgcc agcgcgtcca tcgcccaagt acacaaagcc
      661 cgcctgcatt cgggcgaacg agtggcggtc aaagttttgc gccccaatct tttgcccctt
      721 atcgaacagg atttgtcgct gatgcgtttc ggcgcggctt gggtcgagcg tctgtttgcc
      781 gacggcaggc gtctgaagcc gcgcgaagtg gtggcggagt tcgacaaata tctgcacgac
      841 gagttggatt tgatgcgcga agccgccaat gccggacaac ttggacgcaa tttccaaaac
      901 agcgatatgc tgattgtgcc gaaggtgttt tacgactact gcaccagcga cgtgctgacc
      961 atcgaatgga tggacggcac gccggtttcc gacatcgcca aactcaaagc agacggcatc
     1021 gatttgcaca aactcgccga ttacggcgtg gaaatctttt tcacgcaggt cttccgcgac
     1081 ggctttttcc acgcggatat gcaccccggc aatattttgg ttgccgccga caaccgctac
     1141 atcgccctcg atttcggcat cgtcggcacg ctgaccgatt acgacaaacg ctacctcgcc
     1201 atcaacttcc tcgccttctt caaccgcgat taccggcgcg tcgccaccgc ccacatcgaa
     1261 tcgggctggg tgcctgccga cacgcgtgcg gaagaattgg aagccgccgt ccgcgccgtg
     1321 tgcgaaccgg tgttcaacaa accgatttcg cagatttcct tcggcttggt gctgatgcgc
     1381 ctgtttgaag tcagccgccg cttcaatgtc gaaatccagc cgcagctggt attgctgcaa
     1441 aaaacgctgc tcaacatcga aggcttggga cggcagcttg atcccgattt ggacttgtgg
     1501 aaaaccgcca aaccgttttt ggtgaaatgg atgaacgggc aggtcggccc taaagccctt
     1561 tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa tcattccttc attgccgcgc
     1621 aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa tgcgtgatgc ctatatccat
     1681 ttggtcaaag tgcagcagcg gcaaagcctg tggctggcgg cgatagcggt tgttttgctg
     1741 ctgattttgc ttttgaaata ggttttgtcc gaatcattgc ccgactccgc ccgtttataa
     1801 ggaaaccggt tatagtggat taacaaaaac cagtacag
//
LOCUS       DAXIEB010000185         1786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000185 DAXIEB010000000
VERSION     DAXIEB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.185"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(100..423)
                     /locus_tag="ACFOA3_002038"
     CDS             complement(100..423)
                     /locus_tag="ACFOA3_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6898974.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(411..650)
                     /locus_tag="ACFOA3_002039"
     CDS             complement(411..650)
                     /locus_tag="ACFOA3_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6898975.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(773..1570)
                     /gene="truA"
                     /locus_tag="ACFOA3_002040"
     CDS             complement(773..1570)
                     /gene="truA"
                     /locus_tag="ACFOA3_002040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6898976.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          406 a          429 c          510 g          441 t
ORIGIN      
        1 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttaaagtt
       61 aatccactat aatgaacaca atccattcag actattcaat caggcaaaca tctcctgcaa
      121 tactgcaaac agtttttcag ccgtactgtt gtctaaattt ccaagatgtt tgaccaatcc
      181 ggctttatcc acagccctaa tctgttcggg caaaagcaaa ccgtctttat cctgaaagcg
      241 gacattgacg cggaacgggg caggacggct tccgctcgtc atgggaacga tcagcacagt
      301 cttgagatag ttgtgtattt caggaggaga gactacgaca caaggacgtg tctttttgat
      361 ttcgcttcct acggtcgggt ctaaggagac cagatagatt ccgccgcgta ctaccatatc
      421 cattctttat ccgcttcgtt ttcaatttcg gaaaaaaaat gctcctgctc ggtttcgaca
      481 agcattgcgg cagcttctgc ccatcccctg cgaacagtag gacagcttaa aataatattg
      541 cccttttcaa ctgtaacagc caagctgtcc actgccccta tttgacccaa taatgatttg
      601 ggcagaatca caccttgcga gtttcccatt ttgcgtatgt tgagaatcat atacgtacca
      661 tatttacctg tttatgtaat aacaatgtta gtaccttgat gaggtagtgt caacatggaa
      721 aaagattgcc tgacagtttg tccgatttca aaatctccgc gacaagaatg ttttaaagcc
      781 attcggggat ttgggggcgg atgatgccgt atgcctcggg atagtcgacg ccggtcaggt
      841 aaagcccgtc gggcatgaag gtcggcgggg ctttgaggcg gctgcgttct tgaatcagtg
      901 cggcgaagcc ttcgacgctg agtctgccgc tgccgacata gacgagcgca cccatgatgt
      961 tgcgtaccat gtggtgcaaa aaggcgttgc cgtgcaaatc gaggcggacg agtcctgagc
     1021 tttgggtaag gtcggcgcgg tagatggttt tgacggggga ttttgcctgg cattcggcgg
     1081 cgcggaagct ggagaagtct tgttcgccga tcaataaggc ggcagcctgc cgcatctgcc
     1141 cgatgtcgag tttgaggtgt gtccagcctg ccctgttttt gagcaggggg gaacggacgg
     1201 gggcggattc gagcaggtag cggtaatgcc gtccgtatgc gtcgaagcgg gcgtgaaatt
     1261 cgggggcgac ctgtcgggcg tgcaaaacgg caatgccttc gggcaggtgg gcatttacgc
     1321 cgcgcaccca tgcctgaacc ggacggacgg cggcagtatc aaaatgaacg acttgggcag
     1381 tcgcatgaac gccggtatcg gtcctgccgg caacggtggt ggaaaccgct tcccctgcta
     1441 tttgggcgag cgcggtttcc aatgccgcct gaacggtcgg tacgccgtca gcctgtttct
     1501 gccagccgta aaagcggctg ccgtcatagg atagggttat tgcccagcgt tgtttttgtg
     1561 cggtatccat cggatttggg attcggataa atgttcagac tgcattgtat cgcagatttt
     1621 gcagggaaac ggcaaacgcc cagggcgagc ggcgttgttt ggggagttgt tggggggggg
     1681 gatgcagttg ctacgaatcg ctatcctgtg aatttaccct gtcaggagtg cccgaatcgt
     1741 cattcccgcg caggcgggaa tctaggacgt aaaatctaaa gaaacc
//
LOCUS       DAXIEB010000186         1731 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000186 DAXIEB010000000
VERSION     DAXIEB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.186"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..555
                     /gene="mafB"
                     /locus_tag="ACFOA3_002041"
     CDS             <1..555
                     /gene="mafB"
                     /locus_tag="ACFOA3_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6898977.1"
                     /translation="NSARQWADAHPNITATAQTALAVAEAAGTVWRGKKVELNPTKWD
                     WVKNTGYKTPAVRTMHTLDGEMAGGNRPPKSITSNSKADASTQPSLQAQLIGEQISSG
                     HAYNKHVIRQQEFTDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWDDKTGTIVI
                     RDKNSDDGGTAFRPTSGKKYYDDL"
     gene            565..771
                     /locus_tag="ACFOA3_002042"
     CDS             565..771
                     /locus_tag="ACFOA3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898978.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            799..1154
                     /locus_tag="ACFOA3_002043"
                     /pseudo
     CDS             799..1154
                     /locus_tag="ACFOA3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1151..1522
                     /locus_tag="ACFOA3_002044"
     CDS             1151..1522
                     /locus_tag="ACFOA3_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6898979.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            1608..1731
                     /locus_tag="ACFOA3_002045"
                     /pseudo
     CDS             1608..1731
                     /locus_tag="ACFOA3_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
BASE COUNT          614 a          299 c          361 g          457 t
ORIGIN      
        1 aattccgcca gacaatgggc tgatgcccat ccgaatataa ctgcaacagc ccaaactgcc
       61 cttgccgtag cagaggccgc aggtacggtt tggcgcggta aaaaagtaga acttaacccg
      121 actaaatggg attgggttaa aaataccggc tataaaacac ctgctgttcg caccatgcat
      181 actttggatg gggaaatggc cggtgggaat agaccgccta aatctataac gtccaacagc
      241 aaagcagatg cttccacaca accgtcttta caagcgcaac taattggaga acaaattagt
      301 agtgggcatg cttataacaa gcatgtcata agacaacaag aatttacgga tttaaatatc
      361 aattcaccag cagattttgc tcggcatatt gaaaatattg ttagccatcc aacaaatatg
      421 aaagagttac ctcgcggtag aactgcgtat tgggatgata aaacagggac aatagttatc
      481 cgagataaaa attctgacga tggaggtaca gcatttagac caacatcagg taaaaaatat
      541 tatgatgatt tataggaaaa agccatgaat atactatcca taaataatca aaactcaact
      601 atttcactaa ctcaagatga agtttttgtt ttacgagcta tcttgaatga gatatatgcg
      661 ggcgtatgtg tagattcaag agaatttgaa aatgtatctg gtgttagaaa acatgaagta
      721 gataatttac aacaacagtt tgctggaatt tataaaaaaa tgacaactta acaacccaaa
      781 tttttgtcag agcctagtgc aaattacaac tatgattcta ttgtagccga aatgaaagaa
      841 aaaaatcatg ggttggcgac agggttgatg ttgttaatat gcctgatgga gcacctacta
      901 gtatggataa cacgcgtatt atggcagcac gtgaagcagg ggtaaaagtg gaagcgaatg
      961 ttcataattt taatgaccga ttatcatcaa aagagagaat caggtttaag catgatggta
     1021 ttgagcctca aacttgggga gaagctatcc agctacgaat tagaaagcaa gaaacacaaa
     1081 aaggagttcc agaagggtgg agcaaaagat ttcctaacgg aagtatttat gatgtaaagg
     1141 tacttaggaa atgataaaac aaaatagttt tgttccgtat cctgaagcaa tgcttcctaa
     1201 aggatttaaa tatccgcaaa gttatttaaa attagctcaa tccactcatg ccattaacta
     1261 cgatgaacaa tattcttttc cttggtggtt tgaaaatgca gaaagcaata tatcagaagt
     1321 aattgacatt tattttgaaa taactggcat tccaaaccta ttaccttttg ctagaaacca
     1381 agagtgggct gcctgttttg atatttcaga taaatcaggt aatcctaaaa ttatagtagt
     1441 taatttagat aatacaaaat attacgagac ttttgaaaat tttgatactt ggctaaaaga
     1501 agctgaaaat gatggttggt agcagccgta ggtcggattc tcgaatccga cgtttttcaa
     1561 cagcggcatt tcggaaacga tagacgcgtc aaatattttt gtcggataca agtatccgac
     1621 catacggcaa tcatacgggt aactccgccc catccgtaga gactgataac agtcatgagg
     1681 ggtatggata cagcgatgaa gtagtgcgac aacatagaca agggcaacct t
//
LOCUS       DAXIEB010000187         1720 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000187 DAXIEB010000000
VERSION     DAXIEB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.187"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            342..1646
                     /locus_tag="ACFOA3_002046"
     CDS             342..1646
                     /locus_tag="ACFOA3_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6898980.1"
                     /translation="MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          431 c          507 g          428 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcg ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttaatcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccgaca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctgccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatccgca cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acgggttcgt tatggcggca tacagcgggc tgatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggataaa
//
LOCUS       DAXIEB010000188         1702 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000188 DAXIEB010000000
VERSION     DAXIEB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.188"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            62..1435
                     /locus_tag="ACFOA3_002047"
     CDS             62..1435
                     /locus_tag="ACFOA3_002047"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6898981.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1615..>1702
                     /locus_tag="ACFOA3_002048"
     CDS             1615..>1702
                     /locus_tag="ACFOA3_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6898982.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYS"
BASE COUNT          437 a          557 c          409 g          299 t
ORIGIN      
        1 ttccccatcc tatcggtttc ccgcttcaga cggcataagg tctgaaagaa agactacaat
       61 tatgagtaat ccattttcct ctttaggttt gggtacggaa ctcgtttccg cactgaccgc
      121 gcaaggttac gaaaacccga cgcccatcca agccgccgcc attcccaaag cactcgccgg
      181 tcatgattta ctagccgccg cgcaaaccgg cacgggcaaa accgccgcct ttatgctgcc
      241 ctcgctcgaa cgcctcaaac gttacgccac cgccagcacc tcgcccgcga tgcaccccgt
      301 gcgtatgctc gtgttgaccc ccacgcgcga actcgccgac caaatcgacc aaaacgtgca
      361 gtcctacatc aaaaacctgc cgctgcgcca caccgtcttg ttcggcggta tgaatatgga
      421 caaacagacc gccgatttgc gtgccggctg cgaaatcgtc gtcgccacag tcggacggct
      481 gctcgaccac gtgaaacaaa aaaacatcag tttgaacaaa gtcgaaatcg tcgttctgga
      541 cgaagcagac cgcatgttgg atatggggtt tatcgacgac atccgcaaaa tcatgcagat
      601 gctgcccaaa caacgccaaa ccctgctctt ttccgctacc ttctccgcac cgatacgcaa
      661 actggcgcaa gacttcatga acgcgcccga aaccgtcgaa gtcgccgcgc aaaacaccac
      721 caacgccaac gtcgaacaac acatcatcgc cgtcgatacc attcagaagc gcaacctgct
      781 cgaacggctg attgtcgatt tgcatatgaa ccaggtcatc gtgttctgca aaaccaaaca
      841 aagcgtcgac cgcgtaacgc gcgaactggt gcgccgcaac ctgtccgcac aggcgataca
      901 cggcgaccgt tcccaacaaa gccggctcga aacactcaac gccttcaaag acggcagcct
      961 gcgcgtcctc gtcgccaccg acatcgccgc gcgcgggctg gacattgccg aactgccctt
     1021 cgtcatcaat tacgaaatgc ccgcccagcc cgaagactac gtccaccgca tcgggcgcac
     1081 ggggcgcgcg ggcgcggacg gcgtggcgat ttccctgatg gacgaatccg aacagaaaat
     1141 gtttgaatcc attaaagagc tgaccggcaa caagctgctc atcgagcgca tcgagggctt
     1201 cgagccgcaa tggtgggaac agggcggcgc aaaaccggaa aaaaccgaaa tgcgcgaacc
     1261 gagacaacgc aaccgctacg aatccgccag ggctcagcgc gaaaaaaata cccgaccgga
     1321 aaccacggca aacgatgcgg gcgcggcttg cggaaaaatt gccggacgca gccgccgaag
     1381 ccgtcgggaa caccggacgt gcgccctgct ccaaccgcgt tacggcgtaa aataaccctg
     1441 aaaatcaaat gccgtctgaa catttcccgt ttcagacggc atttttcaaa ccggactgac
     1501 gcatcgggag caaccgcccg caccggataa atttcttccg caaacagttt cagacggcat
     1561 ttgctgcctg tacaatatca tttccttttc cactcaaacc atcaggattt cccaatgtcc
     1621 acccaattcg atgtcgccgt aatcggcgca ggtccggcag gctcggtcgc atccgccctg
     1681 ctccgcaaaa aaggttatag tg
//
LOCUS       DAXIEB010000189         1687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000189 DAXIEB010000000
VERSION     DAXIEB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.189"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(477..1400)
                     /gene="lpxC"
                     /locus_tag="ACFOA3_002049"
     CDS             complement(477..1400)
                     /gene="lpxC"
                     /locus_tag="ACFOA3_002049"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6898983.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            1392..>1687
                     /locus_tag="ACFOA3_002050"
     CDS             1392..>1687
                     /locus_tag="ACFOA3_002050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898984.1"
                     /translation="MQHKPLPYQWGKFWNNTIKPEKQAMFFHSICQCIRFQYVSRIKM
                     YSGLTKIKTRRRTRLPPEKRKKCRPKTFRTAFSREPSFSQGHKTKTGNQKQQPK"
BASE COUNT          462 a          417 c          396 g          412 t
ORIGIN      
        1 cggagcggtt tctgttgctc ccgataaatt cctaaaactt aaaatttcat cattcccaca
       61 aggacaaaaa accaaaacag aaacctaaaa ttcgtcattc ccgcgcaggc gggaatccag
      121 cctcgtcggt gcggatgctt atcggataaa acggtttctt tagattttac gttctagatt
      181 cctgcctgcg cgggaatgac gaatccatcc atacggaaac ctacaccacg tcattcctac
      241 gaacctacat cccgtcattc ccacaaaaac agaaacctca aatcccgtca ttcccgcgca
      301 ggcgggaatc tagaccttag aacaacagta atattcaaag attatctgaa agttcgggat
      361 tctggattcc cacttccgtg ggaatgacga attttaggtt tctgttttgg ttttttgtcc
      421 ttgtaggaat gatgaaattt taagttttag gaatttaccg gaaaaaacag aaagcgttat
      481 ccacaagttc tgatgttcag ctcgtgaaat gcgtcgggca aatcatcgct gtcggcaaat
      541 tccacccggt cgtaagccgt ttcgtctgcc aaaaccgcgc gcaagagtgc gttgttgatg
      601 gcgtgtcccg atttgtagcc ttcaaacgcg ccgacaatcg gatgtccgac gatatacaaa
      661 tcaccgatgg catcaaggat tttgtggcgc acaaactcat cgggatagcg caagccttca
      721 ggattcagga catccgtgtc gtcaatcacg atggcgttgt tcaaattgcc gcccaaaccc
      781 agattgtggg cgcgcatcat ttccacttcg tgcataaagc cgaaagtgcg cgcgcgcgcg
      841 atttcgtcga tgtaggattt gccggcgaaa tcaatttcaa aagtgggcga gctgcggttg
      901 aaaaccggat ggtcgaattc gatggtcagc gttaccttaa agccgtcata cggcgtaaag
      961 cgcacccatt tgcccgcttc tttgatttcg acaggcttga ggattttcaa aaaacgcttt
     1021 tgcgcctttt gatcgaccac gcccgcatct tgcaaaaggt aaataaacgg caggctggag
     1081 ccgtccataa tcgggatttc gggcgcgttc agctcaatca gcgcgttgtc gataccgtag
     1141 gcggacagcg cggacataat gtgttcgatt gtgccgacgc gcacgccttt gtcggtaacg
     1201 atggtggagg aaaggcgggt atcgttgatc aaataagggg tcagtttgat ttgttcgccc
     1261 atctcgccgt ccaaatcggt gcgtcggaag gaaatcccgc tgttttcagg cgcggggtgc
     1321 agggtcagcg cgacgcgttc gcctgaatgc aacccgactc cggtaacgct gatggatttc
     1381 gccaaagttc tttgcagcat aaaccgctcc cttatcaatg gggtaagttt tggaataata
     1441 cgataaaacc ggaaaaacaa gctatgtttt tccatagtat ttgccaatgt atccgttttc
     1501 aatacgtaag ccgcataaaa atgtatagtg gattaacaaa aatcaagaca aggcgacgaa
     1561 cccgcctccc tcctgaaaaa cgcaaaaaat gccgtccgaa aacctttcgg acggcatttt
     1621 cgcgtgaacc gtcattctca caaggacata aaaccaaaac aggaaaccaa aaacagcaac
     1681 ctaaaat
//
LOCUS       DAXIEB010000190         1542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000190 DAXIEB010000000
VERSION     DAXIEB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.190"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..504)
                     /locus_tag="ACFOA3_002051"
     CDS             complement(<1..504)
                     /locus_tag="ACFOA3_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6898985.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQQIEQYLNRQRTR
                     YLRDHRGRPACPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINCQLTEKGLKVEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGQVS"
     gene            857..>1542
                     /locus_tag="ACFOA3_002052"
     CDS             857..>1542
                     /locus_tag="ACFOA3_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6898986.1"
                     /translation="MGGTPAEAQTGGAVGQNAVENNLYLTSEALKKDKQTARKIYSVI
                     KEQVKHECSSTGRITECRQNIGRIIEFTQDQRFDSRFKDLKKESLYYLNKHPDLVASY
                     LKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFGLGCAAALVG
                     VTSSYDHVITGTKNFGKKASEQRPTITVQTLKQLGLSEQAAEYVQFSIDLFSVGKSGG
                     GIPKAKPVSDA"
BASE COUNT          386 a          341 c          451 g          364 t
ORIGIN      
        1 gctgacttgt ccttcttcgt caacttctat ggcctgacgc tgtttgctgc cggcggtctg
       61 aataatggtg gcgtcaatga cggcggcgga tgctttctct acttttaagc ctttttcggt
      121 cagttggcag ttaatcagtt ccaacagttc ggacagggtg tcgtcttgcg ccagccaatt
      181 gcggtagcgg cataaggtgc tgtaatcggg gatgcttagt tcgtcaaaac ggcaaaacag
      241 gttgaaatcg atgcgggtga tgaggctgtg ttcgagttcg ggatcggaga gactgtgcca
      301 ttgtccgagc aggacggctt tgaacatgga caacagggga caggcgggac gaccgcggtg
      361 gtctcggagg taacgggttc tttgacgatt caggtattgt tcgatctgct gccaatcaat
      421 cacttgatcc aacttcaata gcgggaaacg gtcgatgtgt ttggcaatca tggcttgggc
      481 ggtttgctgg aagaaggtgc tcatgagaaa tctcctaaat gtcttggtgg gaatttaggg
      541 gattttgggg aattttgcaa aggtctccgc ccaagccgtc ggacatttcc ttttaggagc
      601 agctatcgcc cgcgtcaacg gtggtaattt tgctgccggc ggctcggcag cagttgcagc
      661 tgaaaaggcg gcggaacatc ttgcccaacg gtataacgac ggtaaaaccg caatcgatcc
      721 gcaaacaggc gagttcaatg ccaacctgct gccggagcat atcaaagaag aaatcaaatc
      781 gaagagcggg gtgattgcat cgctgacggg agcggccgtg ggcggcacgc cggcagaggc
      841 gcaaaccgga gcggccgtgg gcggcacgcc ggcagaggcg caaaccggag gggctgtcgg
      901 gcagaatgcg gtggaaaaca acctctatct gacatcggaa gccttaaaga aggacaagca
      961 gacagctcgt aaaatttatt ccgtcataaa agagcaagtc aagcatgaat gcagttccac
     1021 aggaagaatt accgaatgtc gtcaaaatat aggacgcatt atcgaattta cccaagacca
     1081 acgctttgac agccgattta aggacttgaa aaaagaatcc ttatattacc taaataaaca
     1141 tcctgattta gtagcctctt atttgaaggc tgaatacgaa aagctggata gggaagacaa
     1201 aagtatcctg caccgctaca tctcacccgg ggctgaaatc gtttcgggca gtttgggtgt
     1261 tgttctttca ggcgtagccg gaggcggatc ttgtgccgag actttcggct taggctgtgc
     1321 cgccgctttg gttggtgtaa cgtcttccta cgatcatgtc attactggga caaaaaactt
     1381 cggaaaaaaa gccagcgaac aacgaccgac gattacggtc cagaccttga agcagttggg
     1441 gttgtcggag caggctgcag aatatgttca gttctctata gatttgttca gtgtgggtaa
     1501 atcggggggc ggtataccta aggctaagcc tgtgtctgat gc
//
LOCUS       DAXIEB010000191         1537 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000191 DAXIEB010000000
VERSION     DAXIEB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.191"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(178..1167)
                     /gene="porB"
                     /locus_tag="ACFOA3_002053"
     CDS             complement(178..1167)
                     /gene="porB"
                     /locus_tag="ACFOA3_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6898987.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVGNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          405 a          348 c          390 g          394 t
ORIGIN      
        1 gaataaaaaa gactaaaaac agaaaaaagc caaacagaaa aaagccaaac agaaaaaagc
       61 caaacagaaa aaagccaaac agaaaaaagc caaacagaaa aaagccaaac agaaaaaagc
      121 caaacagaaa aaagcctgtc tggcgacagg ctttttgttg ataccaatct ttgcagatta
      181 gaatttgtgg cgcagaccga caccgccggc agtcgatacg aatttgcttt cgcctttgcc
      241 ttcttgcaac caaccggcag aaaccaaggc agaagtgcgt ttggagaagt cgtattccgc
      301 accgacaacc acttggtcgt aatcgttggt gtagtctgca tcatcaaacg agcctttgaa
      361 gccgtgggcg taagaaacgc ggggcgttac gttgccgaag cggtatgcca aggtagcggc
      421 aacttcggtt tgagagttgt gcgaattgtc ttcaaccagt ttcgcgtctt gttgctgtac
      481 ggctacggaa gcgtataggg catcattgtc gtaaccgctg accaaacggt gaatctggta
      541 tttctcaatc ttcacgttac ccacatcctg atgtctttta taggcaccgc catattgcac
      601 gaagaagccg ccgtttttgt agttgaagcc ggcatggtaa gattcgctgt tatgtctgcc
      661 tgcattgtcg ttaagcgcgt attgtacgct gccgctgagg ccggcaaatt cgggagaatc
      721 gtagcgtacg gaaatgaggc gtgcttcggg ttcggcaatt ttgtttacac ccaaatagtc
      781 gcttttgcta tcccaaggat tgatgtcgcc ggtgtctttc aggacgctgt tcaaacggcc
      841 gacgcgcaat ttaccgaagc cgcctttcaa accgatgaag gattggcggt tgccccaacc
      901 ggagtcagta ccggcgatag atgccttttg ctcaacctgc caaatggctt tcaggccgtt
      961 gccgaggtct tcttggcctt tgaagccgat tttcgaaccc aaatcaacga tgccggtacc
     1021 ggtttcaacg ctagccgcct gagctccatt gtgagctacg gagcgggaag tttctacgcc
     1081 ggctttgatg gtgccgtaca gggtaacgtc agccattgct gcaacaggaa gggctgccaa
     1141 agtcagggca atcagggatt ttttcattgc tgtattcctt ttttggttaa gaaatttaag
     1201 caggccgggc tttccaagcc gcttagcttt gcatttaccg ccgacgtttt cggcgattcc
     1261 atgtctgaaa ctatagatgt tcctgtctga aaaaacaaac ttctgcctgt ttttgtgtgg
     1321 aattcgatgt gtattttgaa gggcggatag agatattgtg ggtatttttt gttttataac
     1381 atatggttat ttaaattttt taagatttgc atttttacaa cacttactcg ggagggtatt
     1441 ggagggcatt gcaaaccggg ggttataaag acggcaaaaa acgcgcacgc ggtttctttc
     1501 ggaacgggcg gatgcaaaac ccgcccctgc ggcagga
//
LOCUS       DAXIEB010000192         1445 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000192 DAXIEB010000000
VERSION     DAXIEB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.192"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            27..1364
                     /locus_tag="ACFOA3_002054"
     CDS             27..1364
                     /locus_tag="ACFOA3_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6898988.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          265 a          345 c          392 g          443 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggcg ggttcggcca tcggtttggg cgcgatttgg aaattccctt
      121 atacggcggg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttactatct
      181 tggtcgccct gcccgttcag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
      361 attatgtcgt ccacagtttt acaggggcaa tccatgcggg agcggacttt gaagccctgt
      421 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcgggattt cagacggcat cgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
      721 ttacctacgc ttcatatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggccattttc attgtcggca
     1081 tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAXIEB010000193         1440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000193 DAXIEB010000000
VERSION     DAXIEB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.193"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..894
                     /gene="mafA"
                     /locus_tag="ACFOA3_002055"
     CDS             <1..894
                     /gene="mafA"
                     /locus_tag="ACFOA3_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6898989.1"
                     /translation="TLTGIPAHGGGKRFAVEQELVAASSRAAVKEMDLSALKGRKAAL
                     YVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPAYDTTATTKSDALSSVT
                     TSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVF
                     YLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRK
                     LLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFSDITPYGDTTAQNRPDF
                     KQNNGKNPDVGNEVIRRRKGG"
     gene            898..>1440
                     /gene="mafB"
                     /locus_tag="ACFOA3_002056"
     CDS             898..>1440
                     /gene="mafB"
                     /locus_tag="ACFOA3_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6898990.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTD"
BASE COUNT          382 a          543 c          312 g          203 t
ORIGIN      
        1 acactgaccg gcatacccgc ccacggcggc ggcaaacgct ttgccgtcga acaagaactc
       61 gtcgccgcat cgtcccgcgc cgccgtcaaa gaaatggact tgtccgccct gaaaggacgc
      121 aaagccgccc tttacgtctc cgttatgggc gaccaaggtt cgggcaacat aagcggcgga
      181 cgctactcta tcgacgcact gatacgcggc ggctaccaca acaaccccga aagtgccacc
      241 caatacagct accccgccta cgacactacc gccaccacca aatccgacgc gctctccagc
      301 gtaaccactt ccacatcgct tttgaacgcc cccgccgccg ccctgacgaa aaacagcgga
      361 cgcaaaggcg aacgctccgc cggactgtcc gtcaacggca cgggcgacta ccgcaacgaa
      421 accctgctcg ccaacccccg cgacgtttcc ttcctgacca acctcatcca aaccgtcttc
      481 tacctgcgcg gcatcgaagt cgtaccgccc gaatacgccg acaccgacgt attcgtaacc
      541 gtcgacgtat tcggcaccgt ccgcagccgt accgaactac acctctacaa cgccgaaacc
      601 cttaaagccc aaaccaagct cgaatatttc gccgtcgacc gcgacagccg gaaactgctg
      661 attaccccta aaaccgccgc ctacgaatcc caataccaag aacaatacgc cctttggacc
      721 ggcccttaca aagtcagcaa aaccgtcaaa gcctcagacc gcctgatggt cgatttctcc
      781 gacattaccc cctacggcga cacaaccgcc caaaaccgtc ccgacttcaa acaaaacaac
      841 ggtaaaaacc ccgatgtcgg caacgaagtc atccgccgcc gcaaaggagg ataaaccgtg
      901 aaaccgctgc gcagactgac aaacctcctt gccgcctgcg ccgtagcggt cgccgccttc
      961 ggacagcccg ccctcgcagc ggacttggcg caagacccgt tcattaccga taacacccaa
     1021 cggcagcact acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt
     1081 tccgaccgca ccggtcaaat caacgtcatc caagactata cccaccagat gggcaatctg
     1141 ctcatccagc aggcaaatat caacggcaca atcggctacc acacccgctt ttccggacac
     1201 ggacacgaag aacacgcccc cttcgacaac cacgccgccg acagcgcaag caaagaacaa
     1261 ggcaacgttg acgaaggctt taccgtatac cggctcaact gggaaggaca cgaacatcat
     1321 cccgccgatg cctacgacgg cccgaagggc ggcaattacc ccaaacctac gggcgcacgc
     1381 gacgaataca cctatcacgt caacggcaca gcccgcagca tcaaactcaa tccgaccgac
//
LOCUS       DAXIEB010000194         1419 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000194 DAXIEB010000000
VERSION     DAXIEB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.194"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            27..572
                     /locus_tag="ACFOA3_002057"
     CDS             27..572
                     /locus_tag="ACFOA3_002057"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6898991.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGTGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            569..742
                     /locus_tag="ACFOA3_002058"
     CDS             569..742
                     /locus_tag="ACFOA3_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898992.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            745..909
                     /locus_tag="ACFOA3_002059"
     CDS             745..909
                     /locus_tag="ACFOA3_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898993.1"
                     /translation="MGQVEFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            912..1148
                     /locus_tag="ACFOA3_002060"
     CDS             912..1148
                     /locus_tag="ACFOA3_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898994.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            1120..>1419
                     /locus_tag="ACFOA3_002061"
     CDS             1120..>1419
                     /locus_tag="ACFOA3_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898995.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     EKMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTE"
BASE COUNT          362 a          325 c          430 g          302 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggcgcg gtcaacggaa gcgaccgtga ggcggatttg gcgcaggata
      121 tgcgcaacat tgtggcttca atcctgcgta acgattacgg cttaaccgta cgcaccgacg
      181 gcacgggcaa aggcaatatg cccttgcgcg atgcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagctgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctggctta tgcacaagcc ggcggcattg tgtttgagcc gttttttatc agcaacgaca
      481 ctgatttggc cttgtttaag acgaccaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggagcg gcgaaggtat gaaaaagtct ttgattgctt tggctctgtc
      601 tgtcttgaaa ccgcagatgc ctgaatttga gattaagcct gccagtattg gctatttgaa
      661 acaacatccg tctatgcgcc tgggcaagtc gggcgtagcg gctgcgaaac gcgcggcgcg
      721 caaacgcaag aatcgtcgtt aatcatggga caggttgagt tttacgaaaa gatgattggg
      781 ctgtggtcgg ccaaaagccg tgaggcaagt gaacaggcag acttggcggc gtttgaattt
      841 gcggagggcg aactggccaa ttatcaggaa atgctgaaac ggcacctgca aaccaaaagt
      901 gtggaatagc aatgcgtatt ttggatattt ttaaaaaccc ggcgacaggc aatgtgtcgc
      961 actcgaaact gtgggcaaac gttgcctgcg cggcggggac gtttaagttt gtgatgctgc
     1021 ccgatccgtc ggcggagatt tgggcggtgt atttgggcat tgtcggcggc tatgcggtgg
     1081 cgcgctcgtt tgtcagcgtc aaacgtcagg aggtcgagaa tgaatctcgt gaaactgctg
     1141 gcgaataact ggcaaccaat tgccatcatc gcacttatcg gcacgggttt ggcggtgtcg
     1201 caccatcaag gctattataa gtcggcgttt gcgaagcagc aggcggtcat tgagaaaatg
     1261 gaaaaagaca aagcgcaagc cctgctgttg tcggctcaaa actatgcacg cgagctggaa
     1321 caggcgcgtg cggaagctaa aaaatatgaa gtcaaggcac acgctgtcgg catggctttg
     1381 gcgaaaaaac aggcggaagt cagccgtctg aaaacggaa
//
LOCUS       DAXIEB010000195         1381 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000195 DAXIEB010000000
VERSION     DAXIEB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.195"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..594
                     /locus_tag="ACFOA3_002062"
     CDS             <1..594
                     /locus_tag="ACFOA3_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6898996.1"
                     /translation="GKAAISTCMANPSGCTVMVTQAAEAGAGIVTGAVTVGNAWEAPV
                     GALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVS
                     AGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVG
                     KVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            601..1110
                     /locus_tag="ACFOA3_002063"
     CDS             601..1110
                     /locus_tag="ACFOA3_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898997.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1194..>1381
                     /locus_tag="ACFOA3_002064"
     CDS             1194..>1381
                     /locus_tag="ACFOA3_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6898998.1"
                     /translation="MPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAPRLLPS
                     FTDPVVPKLSAPGGYIVD"
BASE COUNT          446 a          261 c          270 g          404 t
ORIGIN      
        1 ggtaaagcag ctatcagcac ctgtatggct aatccttctg gttgtactgt catggttacg
       61 caggctgctg aagcgggcgc gggaatcgtc acgggtgcgg taacggtagg caacgcttgg
      121 gaagctccag taggagcgtt gtcgaaagcg aaggcggcta agcaggctat accaacccag
      181 acagttaaag aacttgatgg cttactacaa gaatcaaaaa atataggtgc tgtaaataca
      241 cgaattaata tagcgaatag tactactcga tatacaccaa tgagacaaac gggacaaccg
      301 gtatctgctg gctttgagca tgttcttgag gggcacttcc ataggcctat tgcgaataac
      361 cgttcagttt ttaccatctc cccaaatgaa ttgaaggtta tacttcaaag taataaagta
      421 gtttcttctc ccgtatcgat gactcctgat ggccaatata tgcggactgt cgatgtagga
      481 aaagttattg gtactacttc tattaaagaa ggtggacaac ccacaactac aattaaagta
      541 tttacagata agtcaggaaa tttgattact acatacccag taaaaggaaa ctaactaaat
      601 atgagtaact ttgaaaaaaa atatatttta gaattaaatg atgctttaag ccatttaaat
      661 cataactcta cctcatttga tttattgaaa gttttgattt catggttatc aaacgatatt
      721 gtcattgata aatttaaaat tttaggttat gactttagta aatatatcga aatgaatccc
      781 gatgactatc cggttgaaaa atctatattg aatagagagg aaattattta tctcaaaaac
      841 aatatttatc gtaaaatatc atcaggaaat tttaaatttc aatactttgt acaatatatt
      901 agagatattt tagaatattt atttattgaa catattgaaa gagtctgtcc ttactgcgaa
      961 tggggtgaaa tgcaaaaatt agaagaacaa aatacgcatg aaacggtgta tctctgtact
     1021 caatgtggat gtgcttttta taacgataat tcacaatttt tattaaaaac ccctttaacc
     1081 attccaatga aacgtgatga atttaaataa acaagccgta gcctgcatga accctaaaat
     1141 ccacgtgtag cgtgtgtgcg ccagcacgca tgcgttccat gatttacggc tcaatgccgt
     1201 ctgaaaagct cacaattttt cagacggcat ttgttatgca agtaaatatt cagattccct
     1261 gtatgctgta cagacgcggg agtgttaagc cccccttgtt tgaagctccg cggctcctgc
     1321 cgagcttcac cgaccccgtt gtgcccaagc tctctgctcc cggcggctac attgtcgaca
     1381 t
//
LOCUS       DAXIEB010000196         1329 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000196 DAXIEB010000000
VERSION     DAXIEB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.196"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..925)
                     /gene="pilC"
                     /locus_tag="ACFOA3_002065"
     CDS             complement(<1..925)
                     /gene="pilC"
                     /locus_tag="ACFOA3_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6898999.1"
                     /translation="MNKTLKRRVFRHTALYTAILMFSHTGGGGQAQAQTQTHKYAIVM
                     NAQNLPEVKWGNQYQLLTHKSNEREVIHTSGFGFAKKHISFSFNNTDEVVAEKKDAVV
                     FGAATYLPPYGKVSGFDTTKLTERKNAVDQIGTTHPGLVGYSYEGSTCSSGGCPTVAY
                     RTQFTFGNSSLAKKTNGGGLDIYEDKSRDNSPIYKLKDHPWLGVSFNLGGESSFKPKR
                     QGSLVYSFSEDVTQQNGADSQHKGKNLVYTTDDYKSQNNKNHQDKHHAVAFYLNAKLH
                     LLDKKHITNIAQVGTVDLGTLKTRIEPTEAWK"
BASE COUNT          295 a          342 c          315 g          377 t
ORIGIN      
        1 ttttccatgc ttccgtcggc tcgatgcgtg ttttcaaggt acccaaatca actgttccga
       61 cttgcgcgat atttgtgatg tgttttttat ccagcaggtg cagcttggcg ttcagataaa
      121 aggcgacggc gtggtgtttg tcctgatggt ttttattatt ctgactcttg taatcgtctg
      181 tcgtataaac gaggtttttg cctttgtgtt ggctgtccgc accattttgc tgcgtcacgt
      241 cctcgctaaa agaatatacc aaagaacctt gtctctttgg tttgaaggag ctctctccgc
      301 ccaaattgaa agacacgccc aaccaaggat gatccttcaa tttgtaaatg ggcgaattgt
      361 cgcggctttt gtcttcgtat atatccagcc cgccgccgtt tgtctttttt gccaaactgg
      421 aattgccgaa ggtaaattgg gttctatagg caactgtagg acaacctccg ctggagcatg
      481 tgctaccttc gtagctgtag cctactaacc cagggtgggt cgtaccaatc tgatcaacgg
      541 catttttgcg ctcggtcagc ttagtggtat caaaaccgga aaccttgccg tagggcggca
      601 gataagtcgc cgcgccgaaa acgacagcat cttttttttc agcaacaact tcatcggtat
      661 tattgaatga gaaactaatg tgcttttttg caaaaccaaa accactcgta tggataactt
      721 cgcgttcatt gcttttgtgc gtcaataact gatattgatt cccccacttt acctcgggca
      781 gattttgcgc gttcattaca atagcgtatt tatgcgtttg cgtttgcgct tgcgcctgcc
      841 cccccccgcc ggtatgggaa aacatcaata tggcggtata aagcgcggta tggcggaaaa
      901 cccgcctttt taaagtttta ttcatcgtat ttccttttcg gttgaaaccc cgccgctcgg
      961 acatccgtcc ttcgggacgg tggaatcgga ctttatccgg gagggatgca gccccttccg
     1021 aatcagggcg gcgccttatg tgccgccctg cgtgttggaa catattttat cccttaaatc
     1081 ttgcttcgcg ggataccgtc ggcggcaggc gcgccgcaaa cggcgggatt cggcaaaccg
     1141 cccgattccg cgcccgccga attgctggtt gaaaagctta cctccccatt ttaactttgc
     1201 acactgatca cggtaaagcg ttttttagca aaccgctgca gatgcccgac agtctggatt
     1261 cccgcctacg cgggaatgac ggtttagaag ttgcccgaaa cctcaaaaaa aaccgaaacc
     1321 gaacaagcc
//
LOCUS       DAXIEB010000197         1316 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000197 DAXIEB010000000
VERSION     DAXIEB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.197"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            152..931
                     /gene="yaaA"
                     /locus_tag="ACFOA3_002066"
     CDS             152..931
                     /gene="yaaA"
                     /locus_tag="ACFOA3_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6899000.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            1014..1104
                     /locus_tag="ACFOA3_002067"
     tRNA            1014..1104
                     /locus_tag="ACFOA3_002067"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1048..1050,aa:Ser,seq:tga)
     gene            1182..1271
                     /locus_tag="ACFOA3_002068"
     tRNA            1182..1271
                     /locus_tag="ACFOA3_002068"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Ser,seq:cga)
BASE COUNT          381 a          312 c          294 g          329 t
ORIGIN      
        1 gacgaagccg aagacagtac agatagtacg gcaaggcgag gcaacgctgt actggtttaa
       61 atttaatcca ctatacttca cttttaatcg gcttgcccgc aaacacgttt aaacttaaaa
      121 tccccgtgtt tgacacaata ccgagcagat tatgtttttt gtcctttccc ctgcgaagaa
      181 ccttaatgaa aaagaccctg cccctgtcag cgagtttacc caacccgacc tgctggcaga
      241 gtccgacatt ctaatgcagc agttgcgcga gcttgcgccg caacagattg ccgaactgat
      301 gcacgtttcc gacaaaattg ccctcttaaa cgcgcagcgc aatgcagaat ggcacacgcc
      361 gtttacgccg gaaaacgcca aacaggcggt ctttatgttc aacggcgatg tttacgaagg
      421 tatggatgca aacacattgg atattggaca gatacgctat ctgcaaaacc atgtccgcct
      481 gctgtccggt ctgtacggtc ttcttcgccc gttagacctg atacagccct accgtttgga
      541 aatggggacg gcatttgcca atttgcgcgg caagaatttg tatgagtttt ggggcgacat
      601 cattaccaac cttttaaatg acacgcttgc ccaagcaggc agcaatacgc ttgtcaacct
      661 tgcctcacag gaatatttca agtccgtcaa cacgaaaaaa cttcgggcgc ggctgattac
      721 cccgatattt aaagacgaaa aaaacggtaa atataaaatc atcagtttct atgccaagcg
      781 cgcgcgcgga ttaatggtgc gctatgcggt agaacatcat attaccgacc ctgaaatgct
      841 gaaaaatttt aattacgaag gctacgcatt caatgacgcg gcttcaaatg aaagcgaatg
      901 ggtttttatg cgttcggaac aaataaagtg aaaacaataa attaagtatt ttccgaaaaa
      961 agtgcttggc aaaatgtata aatttcatta ttattcctaa tcttcaagaa gacggaagcg
     1021 tggcagagcg gtttaatgca acggtcttga aaaccgtcga gggttgatag ccctccgtga
     1081 gttcgaatct caccgcttcc gccaatcctt gaggtcaaaa accaaataaa aatgaagtag
     1141 cataaagcat cggcattttt atttggtttt acttcattat cggaaacgtg gcagagaggc
     1201 tgaatgcagc ggactcgaaa tccgctgagg gtgcaaatcc tccgtgggtt cgaatcccac
     1261 cgtttccgcc agaaaacata accgccctga ttcggggcgg ttcttttttg ttcaag
//
LOCUS       DAXIEB010000198         1284 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000198 DAXIEB010000000
VERSION     DAXIEB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.198"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          308 a          367 c          327 g          282 t
ORIGIN      
        1 aatctcgaaa tccgtcattc ccgcacaggc gggaatccag acccccgacg cggcgggaat
       61 ctatcggaaa tgactgaaac cccgagattc tagattccca cttccgtggg aatgacggtt
      121 cagttgcgtt ccgacaacac cgcaatcttg aaattcgtca ttcccgcgca ggcgggaatc
      181 cagacccctg acgcggcggg aatctatcgg aaatgactga aaccccgaga ttctagattc
      241 ccacttccgt gggaatgacg attcagttgc gttccgacaa caccgcaatc tcgaaacccg
      301 tcattcccgc gcaggcggga atccagaccc ctgacgcggc gggaatctat cggaaatgac
      361 tgaaaccccg agattctaga ttcccacttc cgtgggaatg acgtggtgca ggttccgtat
      421 ggatggattc gtctcccgca caggcgggaa tccagacctg tccgcacaga aacttatcgg
      481 ataaaaacag ttgcccaaac cccgcgttct agattcccac ttccgtggga atgacggttc
      541 agttgcgttc cgacaacacc gtaatcttaa aacccgtcat tcccgcgcag gcgggaatct
      601 agacctccga cgcggcggga atctatcgga aatgactgaa cccccgagat tctagattcc
      661 cactgtcgtg ggaatgacgg ttcagttgcg ttccgacaac accgtaatct taaaacccgt
      721 catttccgac aacgccgcaa tcttgaaatt cgtcattccc gcacaggcgg gaatccagac
      781 ccccgacgcg gcgggaatct atcggaaatg actgaaaccc cgagattcta gattcccact
      841 tccgtgggaa tgacgtggtg caggtttccg tatggatgga tccgtcattc ccgcgcaggc
      901 gggaatctat cggaaatgac tgaaaccccg agattctaga ttcccacttt cgtgggaatg
      961 acggttcagt tgcgttccga caacaccgta atcttgaaac ccgtcatttc cgacaacgcc
     1021 gcaatcttga aattcgtcat tcccgcgcag gcgggaatct aggtctgtcg gtgcggaaac
     1081 gtatcggata aaacggtttc ttcagatttt acgttctgga ttcccacttt cgcgggaatg
     1141 acgggatgta ggttcgtagg aatgacgtgg tgcaggtttc cgtgcggatg gattcgtcat
     1201 tcccgcgcag gcgggaatcc agccccctga cgcggcggga atctatcgga aatgactgaa
     1261 accccgcgtt ctagattccc actt
//
LOCUS       DAXIEB010000199         1281 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000199 DAXIEB010000000
VERSION     DAXIEB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.199"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..645
                     /locus_tag="ACFOA3_002069"
     CDS             <1..645
                     /locus_tag="ACFOA3_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6899001.1"
                     /translation="GKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPV
                     GALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAG
                     LLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDG
                     RLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            664..1038
                     /locus_tag="ACFOA3_002070"
     CDS             664..1038
                     /locus_tag="ACFOA3_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6899002.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          411 a          244 c          286 g          340 t
ORIGIN      
        1 ggtaaattag ctatcagcac ctgcatggct aatccttctg gttgtactgt catggtcact
       61 caggctgccg aagcgggcgc gggaatcgcc acgggtgcgg taacggtagg caacgcttgg
      121 gaagcgcctg tgggggcgtt gtcgaaagcg aaggcggcta agcaagctgc tcctaaagaa
      181 acaataaaca atttggcaaa tttagccaaa gcagaacagc agattttatt ccgtattgcc
      241 caacgcgata cgcaactgga tgcatggaag acgggattta acaatagagt aaggaaagga
      301 gcaggcttgc ttgatgcaag taatattccg ataaccatta acggaaaaac catcaaacct
      361 gtacaagcca taagcttaaa gggagcaccc gtttacagcg gcgtaagcga acaggagatt
      421 tttgcgcttt atcggcagat gactggccag aatccgaatt ttagagtttt gcctgacgga
      481 agattagcaa atggcattat cagtactgga gaatgggcag gaacaaaaat tgcattaaga
      541 aatttttcaa aaacagagaa ttcaactcaa gcacgatgga cattagattt gcagaatcct
      601 ccatcattta ttaaaggtac taaattggag cttaaattcc aataatttac aaaggatttt
      661 accgtggatg agaaacaaaa aattaagatt cttgattttc aaatcgattt atcctcaatt
      721 tttaactctt ataaaaatca aatgggtatt aatattcaag ataaaaactt aaaaaacaat
      781 ttctgttctt ttatggaaga actgttaaat gacggttcaa tccgtttaca tgattatacc
      841 gacggtatcg gaattcctct aactggaact tcaaaagaac aagtgcagaa attgaaagac
      901 atatggccta ctttggaaga tgcccaagca atatggcctg aagacccttg gtattactta
      961 gaatggcttt ggtgggatat tgcgtgtcca atagatttgg ccgatttgcc gaatattgat
     1021 atttatgagc aagcgtaggt atggttagcc gcctttagcg gcgtaaccgt acgcatatca
     1081 gcaaacttta taaaataaca aggccgtctg aaatctgttt ttcaactttt tcagacggcc
     1141 ttgcaacttg gcatttcatt cgtacggtta cgccgctaaa ggcggctaac cgtacctacg
     1201 agctctgata aaaatgattt atggaagcaa gctgtagcct gcatgaaacc taaaatccat
     1261 gcgtaaggtg tgtgcttcag c
//
LOCUS       DAXIEB010000200         1227 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000200 DAXIEB010000000
VERSION     DAXIEB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.200"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            275..>1227
                     /locus_tag="ACFOA3_002071"
     CDS             275..>1227
                     /locus_tag="ACFOA3_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899003.1"
                     /translation="MEIFEVNVYTKYNPKDPKQNIIGTVKASYVSDGAGGYLKDKNGN
                     PYIAPVGYNPHEAWAYGAENPLNLLNYGTGKEKDLQRSFHGKSYGGFVPAFQPIASFD
                     VGVACAGAIGVSKDICIAGGGAVNLRNSLSNSNINTEGKYYNNPPNVPNIENGYDWGK
                     NLGTFNDSLENSLKETLNNIRNKFKEIQNSFHNLTDRNISFDSESIGSLLKEKFEDWS
                     HRVYDWLPDDWNLRKELYRNRSGKYHVYDPLALDLDGDGIETVATKGFAGALFDHRNQ
                     GIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADG"
BASE COUNT          389 a          212 c          231 g          395 t
ORIGIN      
        1 tacaaaatct atctatatgg caattattta tcctatttta tattttttat cgtaaaaaaa
       61 tattatcctt actctaggaa agtgataatt ctattatcat tatcattatc tatatatttt
      121 acttttatgg acttttactt tttttccata tattcagata accttagcta tgaaacagaa
      181 cctttacatt tatacatccc tattattatt aactttttct cacttttagt ttctaatttt
      241 attttatctt ttatcaacaa gtaaaggctc agttatggaa atttttgagg taaatgtata
      301 tacaaaatat aatccaaaag atcctaagca aaatattatt ggtacagtta aagcttcgta
      361 tgtttcagat ggagctggtg gttatctaaa agataagaat ggtaatcctt atattgctcc
      421 cgtcggatac aaccctcatg aagcatgggc ttatggagcg gagaatccac ttaatctact
      481 aaactatggt acaggtaaag aaaaagattt gcagcgttca ttccatggaa aatcgtatgg
      541 gggatttgtg ccagcgtttc agcccattgc atcttttgat gttggagtag cttgtgctgg
      601 tgcaattggt gtttctaagg atatttgcat tgctggaggt ggagcggtaa atttaaggaa
      661 ttcgttatct aatagtaata tcaatactga ggggaaatac tataacaacc cgcctaatgt
      721 tccaaatata gaaaatggat atgattgggg aaaaaacctt ggtacattta atgattcttt
      781 agaaaattca ttaaaagaaa cattaaataa tatcaggaat aaatttaagg agattcagaa
      841 ttcatttcat aatttaacag atagaaatat ttcttttgat tctgaaagca ttggcagtct
      901 cctgaaggag aaatttgaag actggtctca tagggtatat gactggctac ctgatgattg
      961 gaatctacgg aaagaattat acagaaatcg ttcaggcaaa taccacgttt acgaccccct
     1021 tgccctagat ttggacggag acggcataga aaccgttgcc accaaaggct ttgcaggtgc
     1081 attgttcgac caccgcaatc aaggcatccg caccgccacc ggttgggttt ctgccgatga
     1141 cggtttactc gtccgcgatt tgaacggcaa cggcatcatc gacaacggtg cggaactctt
     1201 cggcgacaac accaaactgg cagacgg
//
LOCUS       DAXIEB010000201         1177 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000201 DAXIEB010000000
VERSION     DAXIEB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.201"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>1177
                     /locus_tag="ACFOA3_002072"
     CDS             <1..>1177
                     /locus_tag="ACFOA3_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6899004.1"
                     /translation="NLDTVQTGKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSG
                     NNLNAKAAEVSSANGTLAVSAKNDINISAGINTTHVDDASKHTGRSGGGNKLIITDKA
                     QSHNETAQSSTFEGKQVVLQAGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSE
                     TYHQTQKSGLMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQI
                     GSTVSSPEGNNIIHAQSIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAV
                     AQSSKQVGQSKNGRVNAMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQN
                     RQTTQIQASQAQASQIQAGGKTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITL
                     "
BASE COUNT          420 a          319 c          239 g          199 t
ORIGIN      
        1 ttaacctaga tacggtacaa accggcaaac atcaagcaac ccattttgat gccgataacc
       61 atgttattcg cggttcaacg aacgaagtcg gcagcagcat tcaaacaaaa ggcgatgtta
      121 ccctattgtc agggaataac ctcaatgcca aagctgccga agtcagcagc gcaaacggta
      181 cactcgctgt gtctgccaaa aatgacatca acatcagcgc aggcatcaac acgacccatg
      241 ttgatgatgc gtccaaacac acaggcagaa gcggtggtgg caataaattg attattaccg
      301 acaaagccca gagccataac gaaaccgctc aaagcagcac ctttgaaggc aagcaagttg
      361 tattgcaggc aggaaacgat gccaacatcc ttggcagcaa tgttatttcc gataatggca
      421 cccggattca agcaggcaat catgttcgca ttggtacaac ccaaactcaa agccaaagcg
      481 aaacctatca tcaaacccag aaatcaggat tgatgagcgc aggtatcggc ttcactattg
      541 gcagcaagac aaacacacaa gaaaaccaat cccaaagcaa cgaacataca ggcagtaccg
      601 taggcagctt gaaaggcgat accaccattg ttgcaggcaa acactacgaa caaatcggca
      661 gtaccgtttc cagcccagaa ggcaacaata tcatccatgc ccaaagcata gacattcaag
      721 cggcacacaa caaattaaac agtaatacca cccaaaccta tgaacaaaaa ggcttaacgg
      781 tggcattcag ttcgcccgtt accgatttgg cacaacaagc gattgccgtc gcacaaagca
      841 gcaaacaagt cggacaaagc aaaaatggtc gtgtcaatgc catggcagct gccaatgcag
      901 gctggcaagc ctatcaaaca ggtaagagtg cacaaaactt agccaatggt acaaccaatg
      961 ccaaacaagt cagcatctcc ataacctacg gcgaacagca aaaccgacaa accacccaaa
     1021 ttcaagcaag ccaagcccaa gcgagtcaaa ttcaagcagg tggtaaaacc acattaatcg
     1081 ccacaggcgc agcagaacaa tccaatatca acatcgcagg ctcagatatt gccggcaaag
     1141 caggcacaat cctgattgcc gataacgaca tcacact
//
LOCUS       DAXIEB010000202         1174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000202 DAXIEB010000000
VERSION     DAXIEB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.202"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>1174
                     /locus_tag="ACFOA3_002073"
     CDS             <1..>1174
                     /locus_tag="ACFOA3_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6899005.1"
                     /translation="KQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIV
                     VIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQA
                     AVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSS
                     TGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASK
                     IKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSD
                     AEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKP
                     DKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWT"
BASE COUNT          345 a          282 c          312 g          235 t
ORIGIN      
        1 ccaaacagcc cgaatacgcc tacctgaaac agcttcagac ggccaagaac gtcgattgga
       61 aacaggtgca gctggcctac gacaaatggg actataaaca ggaaggcttg accggagccg
      121 gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga gcggctgcag
      181 cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg acagcggcag
      241 caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc tccttgtata
      301 gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca ttaaaagatc
      361 tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg ggtgcattaa
      421 accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa ctgttcagca
      481 gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac ctcagcaatg
      541 caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa gacaacttag
      601 gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc agcaaaatca
      661 aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg gcgggttgtg
      721 ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca gttggggaaa
      781 tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat gcagaaaagc
      841 ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc aacggcggcg
      901 atgtgaatac tgcggcgaat gcggctgagg tagctgtggt gaataatgct ttgaattttg
      961 acagcacccc taccaatgcg aaaaagcatc aaccgcagaa gcccgacaaa accgcactgg
     1021 aaaaaatcat ccaaagtatt atgcctgcac atgcggcagg tgcgatgact aatccgcagg
     1081 ataaggatgc tgccatttgg ataagcaata tccgtaatgg catcacaggc ccgattgtga
     1141 ttaccagcta tggagtttat gcagcaggtt ggac
//
LOCUS       DAXIEB010000203         1171 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000203 DAXIEB010000000
VERSION     DAXIEB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.203"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            51..1109
                     /gene="dinB"
                     /locus_tag="ACFOA3_002074"
     CDS             51..1109
                     /gene="dinB"
                     /locus_tag="ACFOA3_002074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6899006.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          261 a          339 c          324 g          247 t
ORIGIN      
        1 atttgcccga tgccggcagt agaatatccc ttttatcctc agaagagccg atgtcttcac
       61 gcaaaattat ccacatcgac atggacgcat tctacgcatc ggtagagctg cgcgaacagc
      121 cgcatttgaa agggcggccg gtggtcgtcg cgtgggaggg cgcgcgttcg gtgatttgcg
      181 ccgcatcgta tgaggcacgg cagttcgggc tgcattccgc gatgtcggtg gcaacggcga
      241 aaaggctgtg tccgcaagcg gtgtatgtgc cgccgcattt cgatttgtac cgtcaggttt
      301 ccgcgcagat tcacgccgta ttcaggcgtt ataccgattt aatcgaaccc ttgtcgctgg
      361 acgaagccta tcttgacgtt acccgtaatt tcaaaaacat cccttacgcc agtgaagttg
      421 ccaaagaaat ccgtgccgcc atttttgcgg aaacaggttt gactgcatcc gcaggcatcg
      481 cgccgaacaa atttctggcg aaaatcgcgt cggactggcg caagccgaac gggcagtttg
      541 tgttgccgcc gcacaaagtc atggcatttt tggaaaccct gcctttgggc aaaatccccg
      601 gcgtgggcaa ggtaacgctg aaaaaaatgc agtcgctggg tatgcggacg gcgggcgact
      661 tgcgccgttt cgagcgcggc gaactcttaa accatttcgg acgctacgga taccgcctct
      721 atgatttggt gcgcggtacg gacgaacgcc ccgtcaaagc cgaacgcgaa cgcctccaaa
      781 tctccacaga aattaccctg cccgaagacc tgccgctcga gcaggctgcc ggacacctcc
      841 cccatcttgc cgaagacttg tggcggcaaa tcacgcgcaa aaacgtcgaa gcccaaagcg
      901 taacgctcaa gctgaagacc tacgatttcc gcatcatcac gcgcacactg acttattcct
      961 ccgtattgcc cgactgcgca gctctgctgc aggctgcgca aatgttgatg gcgcgcgtcc
     1021 cgccgcagac ggaagacgcg ttccgcctta tcggtatcgg cgtggggcat cttgtgccga
     1081 aaaaccagca gcaggatttg tgggcgtaaa ccgctttacg cgcgccgtcc aaaaaatatg
     1141 ccgtctgaac ggttttcaga cggcatggct g
//
LOCUS       DAXIEB010000204         1149 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000204 DAXIEB010000000
VERSION     DAXIEB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.204"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..202
                     /locus_tag="ACFOA3_002075"
     CDS             <1..202
                     /locus_tag="ACFOA3_002075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899007.1"
                     /translation="GGNLGDFGEFCKGLKRMKSTDIQTQSFLAHFQRIVNAENFVNRF
                     FAQVSASFRGMGNRIRKRTHAT"
     gene            265..936
                     /locus_tag="ACFOA3_002076"
     CDS             265..936
                     /locus_tag="ACFOA3_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6899008.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            998..>1149
                     /locus_tag="ACFOA3_002077"
     CDS             998..>1149
                     /locus_tag="ACFOA3_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6899009.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWHPIEHYLNRQKTR
                     YLRDHRG"
BASE COUNT          330 a          267 c          255 g          297 t
ORIGIN      
        1 tggtgggaat ttaggggatt ttggggagtt ttgcaaaggt ctcaaacgga tgaaatcaac
       61 cgatattcaa acacagtcat ttttagcgca ttttcagcgt atcgttaatg cggaaaattt
      121 cgtgaacagg ttttttgcac aggtctccgc ctcgtttcgc gggatgggaa accgtattag
      181 aaaacggacg cacgcaacat aaaccccgaa agtgatgata agatgatgat ttaacgtact
      241 gctttaatta tttaaggaat tatcgtgttt cccgacaaat acaagttgag tttggaagaa
      301 aatatttttc tggcaaagaa agtattggtt gcccaaattc acaacctcag ccgttttgag
      361 aattgtcaga cgaccttgtt gcagaccgaa caaattatcc atggcaaaaa tgtagcctcc
      421 gcgtcactgg aagacatcca aaccatcttg aacctgaaac gtgcctatca atatgtgatt
      481 tcgcatattt caaatggcga accggtcaat atttcactcc ttaaaaaaat caacaacatt
      541 gttgccaagg acgattcttt ggcacccggt gatttccgta ccggttcggt cggcgtaacg
      601 ctattggacg gttcccgtca tgccccgaat ccagtgaagg aaattgaagt ggcccgcgtg
      661 ttacaaaata tcggactgca aagcggttcg acgacggagg cagccgtccg ttttatgctt
      721 tattgtatgc ggcagcaggt tttttgggac ggcaacaaac gaacggcaac cttatttgcc
      781 aacggtctga tgatggcggg gggctgcggc atcttggaaa tctccgaaat gcagatgccg
      841 caattcaatg aaaaactgtc cgcattctat cgctccggcg acgataccga tatttccaag
      901 tttgtgtatc aaaattgtat atcgggcata gactgagacc tttgcaaaat cccccaaaat
      961 cccctaaatt cccaccaaga catttaggag atttctcatg agcaccttct tccaacaaac
     1021 cgcgcaagcc atgattgcta aacacatcga ccgcttccca ctattgaagt tggaccaggt
     1081 gattgattgg cacccgatcg aacactacct gaaccgtcaa aaaacccgtt accttagaga
     1141 ccaccgcgg
//
LOCUS       DAXIEB010000205         1135 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000205 DAXIEB010000000
VERSION     DAXIEB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.205"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..306
                     /locus_tag="ACFOA3_002078"
     CDS             <1..306
                     /locus_tag="ACFOA3_002078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899010.1"
                     /translation="FILTGGGGIFAKLIIVFICEVLFFVDRFCRKCKKRRKYSGLTNA
                     GMTKSNLSASFPRKRESRTPNVAGIYRKKPKPNRLDSRLRGNDEKQPFRVLPAKAGI"
BASE COUNT          341 a          259 c          276 g          259 t
ORIGIN      
        1 tttatattaa cagggggggg ggggattttt gcaaagctga ttatcgtttt tatttgcgaa
       61 gtgttgtttt ttgttgacag gttttgtcgg aaatgtaaaa aacggcggaa atatagtgga
      121 ttaacaaatg cgggaatgac gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa
      181 tctagaaccc ctaacgtggc aggaatctat cggaaaaaac cgaaaccgaa cagactggat
      241 tcccgcctgc gcgggaatga cgaaaagcaa cctttccgcg tccttcccgc gaaagcggga
      301 atctagaacc cctaacgcgg caggaatcta tcggaaatga ctgaaaccga acgaacctag
      361 attcccgcct gcgcgggaat gacgaaaagc aacctttccg cgtccttccc gcaaaagcgg
      421 gaatctagaa cctctaacgt ggcaggaatc tatcggaaat gactgaaacc gaacagactg
      481 gattcccgcc tgtgcgggaa tgacgaagcc tgcgtgggaa tgacgaaaag caacctttcc
      541 gtgtcattcc cgcaaaagcg ggaatctagg aactcaaagc tgcaagaatt tatcagaaac
      601 aactggaact caaaaaaact agattcccgc ctgcgcggga atgacgaagg gttaaagaaa
      661 tgatgagaaa cggcggtaat ttatcgggaa taaccgaaaa cgaacggaaa tcaggacaag
      721 gcggcgaagc cgcagacagt acaaatagga cggaaccgat tcacttggtg cttgagcacc
      781 ttagagaatc gttctctttg agctaaggcg aggcaacgac gtactggttt ttgttcatcc
      841 actataacag caaccctgtc gccgtcattc ccgcgaaagc gggaatccag tccgttcagt
      901 ttcggtcatt tccgataaat tcctgctgct tttcatttct agattcccac ttttgcggga
      961 atgacggcgg aagggttttg gttttttccg ataaattctt gaggcattga aattctagat
     1021 tcccgcctgc gcgggaatga cggcggagcg gtttctgttt tttccgataa attcctaaaa
     1081 cttaaaattt catcattccc tcaaaaacag aaaaccaaaa acagaaacct aaaat
//
LOCUS       DAXIEB010000206         1127 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000206 DAXIEB010000000
VERSION     DAXIEB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.206"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(230..1084)
                     /locus_tag="ACFOA3_002079"
     CDS             complement(230..1084)
                     /locus_tag="ACFOA3_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6899011.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          309 a          338 c          297 g          183 t
ORIGIN      
        1 cccgtcattc ccgcgcaggc gggaatccgg acatccaacg ctgcagcaat ttgtcggaaa
       61 taaccgaaac tcaaaaaacc ggatgcccgc ctgcgcggga atgacgatgc aaagatggtc
      121 gttatttcgg ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga
      181 tggaaagatt tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat
      241 gcgacagccg gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca
      301 cgggcgacag gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga
      361 gcgaaatcaa cagtcccgac acgttggcag gaacacggct catccccttg ttccacagcc
      421 aataggcgta ccagccgcac cccacgccca aatacagcag cgacaatacc attccgacgc
      481 tccagtccac ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg
      541 cggcggcaat ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg
      601 tcggacgcat agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc
      661 agccgacttc gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg
      721 ccgccgcgcc gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga
      781 caaacaccat cagcagtggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt
      841 atttcaaccc gacaaactga agcagcaggg tcagcacata gttgacgaac gacacaatca
      901 gcaacggctt ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg
      961 caggcagcgc ggcaattagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat
     1021 agacatattt ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa
     1081 acatagtgag tgataaagtc agatggtcat acaaaagtat agcggat
//
LOCUS       DAXIEB010000207         1122 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000207 DAXIEB010000000
VERSION     DAXIEB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.207"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..763
                     /locus_tag="ACFOA3_002080"
     CDS             <1..763
                     /locus_tag="ACFOA3_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6899012.1"
                     /translation="RAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQK
                     EDWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFD
                     RLIDELDKQIDNHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLV
                     GIAPHPRESGETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFHQRPLSEGKPYK
                     VAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
     gene            920..>1122
                     /locus_tag="ACFOA3_002081"
     CDS             920..>1122
                     /locus_tag="ACFOA3_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6899013.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKP"
BASE COUNT          293 a          270 c          304 g          255 t
ORIGIN      
        1 ccgagcaggc atagccgtga ttatcgccaa cccgcgtcag acgcatcagt ttgcccaatc
       61 gcagccgctg accaaaaccg acgccaaaga tgccaaaatg cccgccttct tcacacagat
      121 gatgacgcag aaagaagatt ggcaaaccat gccctaccaa ccgcccaccg aagcggaaga
      181 agtgttggaa gccttggtta accgccgcaa ccaatcggcg gatatgcgga ctgccgagaa
      241 aaaccgtctg catcaggttc atgaaacgca agtcggaagc gtcaaacaac tgattgccca
      301 ttttgaccgg ctgattgacg aattggacaa acaaatcgac aaccacaccc acacgcattt
      361 tgacggcaaa gcccaagtgg cagagcaaat caaaggcatc ggttcgataa cgacggctac
      421 gctgatggcg atgttgcctg agttggggcg gctgtcgcac aaacggatag cgagtctggt
      481 cggcattgcc ccacacccga gggagagcgg ggaaaccaaa ttcaaaagcc gctgctttgg
      541 cggaaggtct gcggtgctta aggcgctgta tatggctacc gtggcagcga cacgttttga
      601 accgcttatt cgggatttcc accaacgccc gctgtccgag ggtaagccgt ataaggttgc
      661 cgttacggca tgtatgcgca aactgctgac gatatcgaat gcccggatgc gtgattattt
      721 tgccgaaaac gataccgccg aaaacggtat ccggacggct tgatttgagt tttggtattt
      781 ttgcccgacg gggtgaaaaa tacagttgct tttttatgtc tgtccgtttc gcaaaaaaca
      841 tcggcttaat actatatatt gtattttatt ggtataatat gcgttaatta gtcaatatat
      901 tgtgttttat gggtttgaga tgcgccgggc gttgattgcg aaaattaaga ttgctcaaaa
      961 ggagctgggc ttggacgacg gtacctatcg cgcggtgttg gagcgtgtga cgggcaagcg
     1021 gtcgtgtgcg gatatggatg tttccgaact tgagtctgtt gtcgctgata tgcggtcgca
     1081 cggatttaag cctaaagcaa aaggtaaccc acacggcaaa cc
//
LOCUS       DAXIEB010000208         1080 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000208 DAXIEB010000000
VERSION     DAXIEB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.208"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            46..726
                     /locus_tag="ACFOA3_002082"
     CDS             46..726
                     /locus_tag="ACFOA3_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6899014.1"
                     /translation="MQADLAYAAERITHNYPEPTGANKDKISTVSDYFRNIRAHSIHP
                     RVSVGYDFGDWRIAADYASYRKWNNNKYSVNTKVLQENNGNREDLKTENQENGTFHAS
                     SSLGLSAIYDFKLNDKFKPYIGARVAYGHVRHSIDSTKKTTDVVTVATNTPDGAPTIY
                     NVPKTQNAYHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEV
                     SLGMRYRF"
BASE COUNT          305 a          325 c          238 g          212 t
ORIGIN      
        1 gcagcgcagg cggcaagtga agacggcagc cgcagcccgt attatgtgca ggcggattta
       61 gcctacgccg ccgaacgcat tacccacaat tatccggaac caaccggtgc aaacaaagac
      121 aaaataagca cagtaagcga ttatttcaga aacatccgtg cgcattccat ccaccctcgt
      181 gtgtcggtcg gctacgactt cggcgactgg aggatagcgg cagattatgc cagttacaga
      241 aaatggaaca acaataaata ttccgtcaac acaaaagtgt tgcaagaaaa caatggcaac
      301 agggaagacc tgaagacgga aaatcaggaa aacggtacgt tccacgcctc ttcttctctc
      361 ggcttatccg ccatttacga tttcaaactc aacgataaat tcaaacccta tatcggtgcg
      421 cgcgtcgcct acggacacgt cagacacagc atcgattcga ccaaaaaaac aacagatgtt
      481 gttaccgtcg ccaccaatac tcctgacgga gcacctacga tttataatgt gccaaagacg
      541 caaaacgcct atcacgaaag ccacagcatc cgccgcttag gtcttggtgt cgtcgccggt
      601 gtcggtttcg acatcacgcc caagctgact ttagacaccg gataccgcta ccacaactgg
      661 ggacgcttgg aaaacacccg cttcaaaacc cacgaagtct cattgggcat gcgctaccgc
      721 ttctgattcc ccgataccga tgccgtctga accttcagac ggcatttttg attcacctgc
      781 cgtttacaga cgcggggcgg gcgtggaaat acccaaaccg tcattcccga caacaccgca
      841 atcttgaaac ccgccattcc cgataacagc gcaatcttga aacccgtcat tcccgcgcag
      901 gcgggaatcc agccccctga cgcggcggga atctatcgga aatgactgaa accccgagat
      961 tctagattcc cacttccgtg ggaatgacgg ttcagttgcg ttccgacaac accgtaatct
     1021 cgaaacccgt cattcccgat aacagcgcaa tcttgaaacc cgtcattccc gacaacaccg
//
LOCUS       DAXIEB010000209         1002 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000209 DAXIEB010000000
VERSION     DAXIEB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.209"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..1001)
                     /locus_tag="ACFOA3_002083"
     CDS             complement(<1..1001)
                     /locus_tag="ACFOA3_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFC6899015.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PLGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSSSNTQTQLGGWIQGNPWLARGEA
                     RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQY
                     QAGDLSGFKIRQGNVAITGQGLDARDTDFTRILSYHSKIDAPVWGQDVRVVAGQNDVV
                     ATGNAHSPILNNAAANTSNNTANNGTHIPLFAIDTGKLGGMYANKITLISTAEQAGIR
                     "
BASE COUNT          263 a          238 c          227 g          274 t
ORIGIN      
        1 cgaatgcctg cttgctcggc cgtactgatc aaggtgattt tgttggcata catacctcct
       61 aatttgcctg tatcaatcgc aaataaaggg atatgtgtgc cgttgttggc tgtattgttt
      121 gacgtattgg cagcagcatt attgagaata ggcgaatgtg cattacctgt tgcgaccaca
      181 tcgttttgtc ccgcgacgac acgaacatct tgtccccata cgggtgcatc aattttggaa
      241 tgataactga gaatacgtgt gaaatcggta tcacgggcat ccaaaccttg tcccgtgatc
      301 gcaacattgc cttgccttat cttaaagccg ctaaggtctc ctgcttgata ttgcggttgg
      361 cctgtcgtca aagtggcacg ggaagcattg ataaaaccac caccattgac tgcaatccct
      421 gccggattgg caataacgac ttctgcacgt cgtccgccca cttcaatata gccattcatt
      481 tgtgaagaat ggctgctgtt gatttggttt acaaccacac gtgcttcgcc ccttgccaac
      541 caaggattac cttgaatcca accgcctagc tgtgtttggg tgttgctgct gctgttgttt
      601 aaaatcgccc cgcgattacc cacatcaaac tgggcgtatt gattaacaga aacccctgcc
      661 gaagtagggg tttgaatatt gacttgcggt atgccgttac ctgtttgcag aatcgtggct
      721 tgttgggttt taggagcagc tttatcagca ataatgccat cagcaaaagc aatattggcc
      781 gtacctacag ccaaacataa agaaaagccc aataaagaaa aagaaaagat atttgaacga
      841 caaacaggtg catgagtagt acccaaagga acagatttca catgagcgct gcctgaatca
      901 ctatcggcac agcttttacc ttcgcgcttg gtagtttcag caacggctac cacagcccca
      961 cgtttgcggt tgaaaattac acgatagaga gttttattca tg
//
LOCUS       DAXIEB010000210          960 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000210 DAXIEB010000000
VERSION     DAXIEB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.210"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>960
                     /locus_tag="ACFOA3_002084"
     CDS             <1..>960
                     /locus_tag="ACFOA3_002084"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6899016.1"
                     /translation="VCVLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLK
                     NGAAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTA
                     FDNSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTH
                     IDDNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLK
                     KFVYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSS
                     GVTIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFR"
BASE COUNT          224 a          288 c          250 g          198 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
//
LOCUS       DAXIEB010000211          899 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000211 DAXIEB010000000
VERSION     DAXIEB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.211"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..265)
                     /locus_tag="ACFOA3_002085"
     CDS             complement(<1..265)
                     /locus_tag="ACFOA3_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899017.1"
                     /translation="MKEGAHGKSPKCLGGNLGDFGEFCKGLNLQIGFKTIRPNSSASK
                     NGMPIYSNVSRREIFDLYEQYSGQRPDFRNIPNKGQLSSTTITS"
     gene            248..899
                     /locus_tag="ACFOA3_002086"
                     /pseudo
     CDS             248..899
                     /locus_tag="ACFOA3_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          262 a          253 c          175 g          209 t
ORIGIN      
        1 ctgatgtaat agtcgtagaa gataattgcc ctttattcgg aatattccta aaatcaggcc
       61 tctgccctga atattgttca tacaaatcaa atatttctct tcgacttacg ttagaatata
      121 tcggcattcc atttttgctg gcagatgaat ttggtctgat cgttttaaaa ccaatttgta
      181 agttgagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttaggag
      241 attttccatg agcaccttct ttcaacaaac cgcccaagcc atgattgcta aacacatcga
      301 ccgtttcccg ctattgaagt tggaccaggt gattgattgg cagccgatcg aacaatacct
      361 gaatcgtcaa agaacccgtt accttagaga tcaccgcggc cgtcccgcct acccgctgtt
      421 gtccatgttc aaagccgtcc tgctcggaca atgacacagt ctctccgatc ccgaactcga
      481 acacagcctc attacccgca tcgacttcaa cctgttttgc cgctttgacg aactgagcat
      541 ccccgattac agtactttat gccgttaccg caattggctg gcgcaagacg acaccctgtc
      601 cgaattgctg gaactgatta actgccaact gaccgaaaaa ggcctaaaag tagagaaagc
      661 atccgccgcc gtcgttgacg ccaccattat tcagaccgcc ggcagcaaac agcaccaggc
      721 tatagaagtc gataacaaag gacaagtcag cggccaaacc acaccgagta aagacagcga
      781 tgcccgttgg acaaagaaaa acggcctcta cagactcggt tacaaacaac atacccgtac
      841 cgatggggaa ggctatatcg agaaactgca catcaccccc gccaatgccc atgagtgca
//
LOCUS       DAXIEB010000212          896 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000212 DAXIEB010000000
VERSION     DAXIEB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.212"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..295
                     /locus_tag="ACFOA3_002087"
     CDS             <1..295
                     /locus_tag="ACFOA3_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6899018.1"
                     /translation="ARMAAKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRD
                     LIVYDPLPADEYQDIFDFMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            292..525
                     /locus_tag="ACFOA3_002088"
     CDS             292..525
                     /locus_tag="ACFOA3_002088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899019.1"
                     /translation="MSRKTLSSCFIDYNFRMNEDWIRQVITNLPDNHPTKNILREAVR
                     SGKIKTAVTGVDRQTGKAVILPVKVPSKANIRR"
     gene            528..>896
                     /locus_tag="ACFOA3_002089"
     CDS             528..>896
                     /locus_tag="ACFOA3_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6899020.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMEHIFMDVRAAASHAYLFEHDLNKFKQYAYVAGKLGVLLSVNAEDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREF"
BASE COUNT          268 a          187 c          189 g          252 t
ORIGIN      
        1 cgcgcgtatg gctgccaaag aaacattgtc ctatttcgat ttctacctgc agccgcaaat
       61 cgttacctat gccaaaatcg catccatgca cggtttcgat ttgggcatag accacgaaat
      121 cgcaccgagg gatttgattg tttacgatcc gctgccagca gacgaatatc aagacatctt
      181 cgattttatg aaacagtatg acttgtctta cccgtatgaa tatctgcagg attggataga
      241 ttactatacg ttcaaaaccg ataagctggt atttggtaac gcgaagcgag agtgagccgt
      301 aaaactctga gctcctgttt tatagattac aactttagga tgaatgagga ttggattaga
      361 caagttatta ctaacttacc agataatcat cccactaaaa acatccttcg agaagcagta
      421 cgttcgggta aaatcaaaac tgcggttact ggagtagatc gtcaaacagg taaggccgtt
      481 attcttcctg ttaaagttcc ttctaaagcc aatataagga gataacaatg gggcacaata
      541 tgatgaccac ccaaaaatgg tatgagcata ttactaatgt aatcataggc aatactgcta
      601 atttcaatag cggttgcctt gactctatag attatgtaga tgaaagaaaa ggcgttccgc
      661 ttgcagctat ggaacatatt ttcatggacg ttagagctgc agcttcccat gcctatctat
      721 ttgaacatga tcttaataaa ttcaagcaat atgcttatgt tgcaggaaag ctgggggttt
      781 tgctgagtgt aaatgccgaa gaccccgaac ccttcttctt tccctgtgac atgctcaaca
      841 ttcaaaatcc gatgtttctg atgctgatga gcgacagccc gcagctgcgc gagttt
//
LOCUS       DAXIEB010000213          853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000213 DAXIEB010000000
VERSION     DAXIEB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.213"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(194..613)
                     /locus_tag="ACFOA3_002090"
     CDS             complement(194..613)
                     /locus_tag="ACFOA3_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899021.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSAIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(644..>853)
                     /locus_tag="ACFOA3_002091"
     CDS             complement(644..>853)
                     /locus_tag="ACFOA3_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6899022.1"
                     /translation="VKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKW
                     QGTSKSGIKIEGFTEPNRTAYPIYE"
BASE COUNT          273 a          172 c          105 g          303 t
ORIGIN      
        1 agagttatta aaaaaataga taatcttaat tattctaatt caaggatttt atctaaatca
       61 aatgcttact gccattcgtt tatgagaact tgtattcaat acaagttcta acatcttaat
      121 catggcacag cgtataagcg tggatttgca gtccaatcta tatcgctgat tctattcatt
      181 aagctaatta tgcttattcc tcaatcacag tctccaccac cgaatgaata cttttaggca
      241 attgcaaaaa agcattccaa gcaattaaag cagttttaaa agtatgcagc gaacagctta
      301 atactggata accatcttct tcatcaaatt cattgtggta aaaaatgacc ttatcatgat
      361 gaaattctag gcaaaattgt tgaccaccgc catcatcata aatttcattt ggattatttt
      421 ctattaattc tataatcttg aaatgctcat caatcgcgga tcgatagtaa atatcgctca
      481 ttaaggtaga cgtaatgtaa tcatatattc ctacataata atcaacccta ttatcatagt
      541 catccagaat gatacacatt ctaaatgtat ctcctgatgg aaatttatca caaaagcact
      601 taattctttt catcattaat ctcctaattt ttcataaata ttactattca taaatgggat
      661 atgctgttct attaggttcg gtaaatcctt ctattttaat acccgattta cttgtaccct
      721 gccatttatt atccttaagc attattctac tttcccaagc cgaagtaact tcagccctaa
      781 ttctagcctc atcccaatct tttgggaaca tggtgtgctt ggtcatcact ttcccacctt
      841 ttttcgtttt cac
//
LOCUS       DAXIEB010000214          832 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000214 DAXIEB010000000
VERSION     DAXIEB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.214"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..751
                     /locus_tag="ACFOA3_002092"
     CDS             <1..751
                     /locus_tag="ACFOA3_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6899023.1"
                     /translation="NIQITDEAAQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNV
                     FDKDRVQSELDLQRTVSQDFSKNVQQANTEINQHLDKLKADKEAAETAAAEALANGDM
                     ETAKHKAHEAQDAAAKADNWQQGKVILNMLASGLAAPTQSGAGIAAATASPAVSYAIG
                     QHFKDLAGQNANGKLTASQETAHVLAHAVLGAAVAAAGGNNAPAGALSAGGSEAAAPY
                     ISKWLYGKEKGSDLTAEEKETETFAKFPKIP"
BASE COUNT          284 a          215 c          195 g          138 t
ORIGIN      
        1 aaacattcaa atcaccgacg aagccgcaca aatccggctg acaggaaaaa cagcggcaca
       61 aaccaaagcc gatattgata caaacgtaac cacagacacc gccgaacgac attcgggcag
      121 cctgaaaaac gtatttgaca aagatagagt gcaaagtgaa ctggatttac aaagaaccgt
      181 cagccaagat tttagtaaaa atgttcaaca agccaatacc gagattaacc aacatttaga
      241 caaactcaaa gcagacaaag aagcagccga aacagcagca gccgaggcat tagccaatgg
      301 cgatatggaa actgccaaac acaaagccca tgaagctcaa gatgcggcag caaaagcaga
      361 taattggcaa caaggcaaag tcattctcaa catgttagcc tcaggtttag ctgcgccgac
      421 ccaaagcgga gcgggcatcg ctgcggctac cgcatcgcca gccgtatcgt atgcgattgg
      481 acagcacttt aaagatttag ccggtcaaaa cgcgaatggc aaactaaccg ccagtcaaga
      541 aaccgctcac gttcttgccc acgcggtatt aggagcagcg gttgcagcag caggaggcaa
      601 caatgccccg gcgggagcat tgagtgcggg cgggtcggaa gcggctgcgc cttacatcag
      661 caaatggtta tatggcaaag aaaaaggaag tgacttaacg gcggaagaga aagagactga
      721 gacctttgca aaattcccca aaatccccta aattcccacc atgacattta ggggattttc
      781 catgagcacc ttcttccagc aaaccgccca agccatgatt gccagacaca tc
//
LOCUS       DAXIEB010000215          821 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000215 DAXIEB010000000
VERSION     DAXIEB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.215"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(637..>821)
                     /locus_tag="ACFOA3_002093"
     CDS             complement(637..>821)
                     /locus_tag="ACFOA3_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899024.1"
                     /translation="PCRRVWIPAFAGMTAVGFRFFPIKSCRVVFLDSRLRGNDAVGVS
                     VFADRFLWFFYGLNHS"
BASE COUNT          251 a          232 c          174 g          164 t
ORIGIN      
        1 caacgatgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttccg ttgtcagaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accacccccc cgaatttgcg taaaataaag tgtaatccct ttgattataa
      181 gggaatgttg acaattagat cgattctcct aggctgactt tcgcaataaa tggggttgtc
      241 aataattgtg tatggtcgtt tgccgtccgg cggccgtcat tcccgcgcag gcgggaatcc
      301 agaaacgcaa cgcagcagga ctttatcgga aaaaaccgaa atcccaccgc cgtcattccc
      361 gcgcaggcgg gaatccagca acacaacgca gcaggacttt atcggaaaaa accgaaaccc
      421 caccgaccgt cattcccgca aaagcgggaa tccagcaacc gaaaaaccac aggaatctat
      481 cagcaaaaac agaaacccca ccgccgtcat tcccgcgaaa gcgggaatcc agacccgtcg
      541 gcacggaaac ttaccggata aaacagtttc cttagattcc acgtcctaga ttcccgcctt
      601 cgcgggaatg acgagatttt agattatggg aatttatcag gaatgattca atccatagaa
      661 aaaccacagg aatctatcag caaaaacaga aacccccacc gcgtcattcc cgcgcaggcg
      721 ggaatccaga aacacaacgc ggcaggactt tatcggaaaa aaccgaaacc ccaccgccgt
      781 cattcccgca aaagcgggaa tccagacccg tcggcacgga a
//
LOCUS       DAXIEB010000216          809 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000216 DAXIEB010000000
VERSION     DAXIEB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.216"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            420..626
                     /locus_tag="ACFOA3_002094"
                     /pseudo
     CDS             420..626
                     /locus_tag="ACFOA3_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            626..809
                     /locus_tag="ACFOA3_002095"
                     /pseudo
     CDS             626..809
                     /locus_tag="ACFOA3_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          222 a          222 c          173 g          192 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacagg aaaaaactac
      121 accaaactac aatttttgtt tatgcaaata tttgttttga caggatttaa acgaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atatagtgga ttaacaaaaa tcaggacaag
      241 gcgacgaagc cgcagacagt acagatagta cggtaaggcg aggcaacgcc gtactggttt
      301 aaatttaatc cactctattg acctgaatat taaagtttcg caaagtaatg caacgttgct
      361 ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcagaga tgttcaatta
      421 tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg actgccgccg
      481 ccgcactctt acccatcgtc tttttctttg ccgcgctgac cgtcctgaag ctgaaaggct
      541 atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgtattc ggcttcggga
      601 tgccggcggg tatggcggtt tcttccctgc cgccgcagcc gcattgaccc aacgccccta
      661 cgccacgctg gatttgaccg cgcattacaa aatcggaaaa tccacacgca tcggtttgga
      721 ctttgaaaac gtgttcaaca aacgctaccg caccatgccc gacattcacg tttacggcac
      781 gccgcgcagc ctgaccgcaa ccgtcaaac
//
LOCUS       DAXIEB010000217          805 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000217 DAXIEB010000000
VERSION     DAXIEB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.217"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..743)
                     /locus_tag="ACFOA3_002096"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="ACFOA3_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC6899025.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAXIEB010000218          699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000218 DAXIEB010000000
VERSION     DAXIEB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.218"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>699
                     /locus_tag="ACFOA3_002097"
     CDS             <1..>699
                     /locus_tag="ACFOA3_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6899026.1"
                     /translation="YLNRQRTRYLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHS
                     LITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKA
                     SAAVVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHT
                     RTDAEGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGYDSAENRQHLEEHQLL
                     DGIMRKACRNRPLTE"
BASE COUNT          201 a          220 c          159 g          119 t
ORIGIN      
        1 tacctgaacc gtcaaagaac ccgttacctc agagaccacc gcggccgtcc cgcctatccc
       61 ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctctc cgatcccgaa
      121 ctcgaacaca gcctcatcac ccgcatcgac ttcaacctgt tttgtcgttt tgacgaactg
      181 agcatccccg attacagcac cttatgccgc taccgcaact ggctggcgca agacgacacc
      241 ctgtccgaat tgctcaaact gatcaaccga caactgaccg aaaaaggcct aaaagtagag
      301 aaagcatccg ccgccgtcgt tgacgccacc attattcaga ccgccggcag caaacagcgt
      361 caggccatag aagtcgatga agaaggacaa atcagcggcc aaaccacacc gagtaaggac
      421 agcgatgccc gttggataaa gaaaaacggc ctctacaaac tcggttacaa acaacatacc
      481 cgcaccgatg cggaaggcta tatcgagaaa ctgcacatca cccccgccaa tgcccatgag
      541 tgcaaacacc tgtcgccgtt gttagaaggg atagctgaag gtacgaccgt ctatgccgac
      601 aaaggctatg acagtgcgga aaaccggcaa catctggaag aacatcagtt gctggacggc
      661 attatgcgca aagcctgccg caaccgtccg ctgacggaa
//
LOCUS       DAXIEB010000219          680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000219 DAXIEB010000000
VERSION     DAXIEB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.219"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..255)
                     /locus_tag="ACFOA3_002098"
     CDS             complement(<1..255)
                     /locus_tag="ACFOA3_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899027.1"
                     /translation="MGIQLFEFQSFPINCLSIECLDSRLRGNDGGAVSVFSSKYPQAE
                     IPLFSQKQKTKNSNLKFVIPAQAGIRFIELQLFRINFETLI"
BASE COUNT          196 a          137 c          165 g          182 t
ORIGIN      
        1 gattagagtt tcaaaattta ttctaaatag ctgaagctca atgaaccgga ttcccgcctg
       61 cgcgggaatg acgaatttca ggttgctgtt tttggttttc tgtttttgtg aaaataacgg
      121 gatttcagct tgtgggtatt tactggaaaa aacagaaacc gctccgccgt cattcccgcg
      181 caggcgggaa tccagacatt caatgctaag gcaatttatc gggaatgact gaaactcaaa
      241 aagctggatt cccactttcg tgggaatgac gaagtggaag ttacccgaaa cttaaaacaa
      301 gcgaaaccga acgaaccgga ttcccacttt cgtgggaatg acgggatgca ggtttccgta
      361 tggatggatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcagtgc ggaaacttat
      421 caggtaaaac ggtttcttga gattttgcgt cctggattcc cactttcgtg ggaatgacgc
      481 gattagagtt tcaaaattta ttctaaatag ctgaaattca atgaaccgga ttcccgcctg
      541 cgcgggaatg acgaagtgga agttacccga aacttaaaac aagcgaaacc gaacgagccg
      601 gattcccgct tgcgcgggaa tgacgggatt aagttttcaa aattcatcag aaattactga
      661 tttaatagca taaatttttt
//
LOCUS       DAXIEB010000220          656 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000220 DAXIEB010000000
VERSION     DAXIEB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.220"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(1..99)
                     /locus_tag="ACFOA3_002099"
                     /pseudo
     CDS             complement(1..99)
                     /locus_tag="ACFOA3_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            120..578
                     /locus_tag="ACFOA3_002100"
     CDS             120..578
                     /locus_tag="ACFOA3_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899028.1"
                     /translation="MPSEHVFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSGGLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          188 a          112 c          176 g          180 t
ORIGIN      
        1 ttgtttggaa gggaaatggc taaaatatgt agcattatgt tctgtatcgt tgtttaccgt
       61 ttccgcacct ttgtccgcct taaagcaggt agacaccgca atgaatcgac gcaaagaaaa
      121 tgccgtctga acatgtgttc gggcggcgtt ttgttggggg gtatcggagc ggaacgtctg
      181 aaaaagggtt tcaggcggtc tttgggcgtg tggtgacagt cgaaaatgtg ataaggctac
      241 ctgaaaagtt tgggagattt tcaggtggcc tttggtattg ggcgcaacag acgcaggtac
      301 agattaggcg gtgtgccgta atcgtacgaa tgccgattca acctaagcag acatcagtat
      361 ttaggaagtg gatgtttgat ggagcaaagg ttgtacgaag gatggaaggc aacctgtggg
      421 tgtttggtat ggtcgcgctt gaaaaaacgt gttttaaggg acaaatgccg tctgaaaatc
      481 ggtttcagac ggcattttct gtttatttaa agcaaacagg aaaaggcagc aatattctgc
      541 agtcttccta ttcacacaag cgttttatag ttaattaaaa acaaaatagt acaatactca
      601 actttgaagg tctaaccatg gcatactctg cggacttaag aaacaaagct ttaaac
//
LOCUS       DAXIEB010000221          654 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000221 DAXIEB010000000
VERSION     DAXIEB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.221"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(52..537)
                     /locus_tag="ACFOA3_002101"
     CDS             complement(52..537)
                     /locus_tag="ACFOA3_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6899029.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRFRNAIEHKKD
                     E"
BASE COUNT          192 a          210 c          121 g          131 t
ORIGIN      
        1 ttcaaaccgg aaaaatgccg tctgaacctt cagacggcat tccattcaca tttattcgtc
       61 ttttttgtgt tcgatggcgt ttcggaaacg attgccgccg taaggctctt caaccgtata
      121 ttccccctcg ataatatcgt catcgcggga aaagccctct tttctgcccg attggttcat
      181 gttgaaaaaa ttttccgcac ctcctgcctg caacactgca cctcccttaa acggcagcag
      241 cagcaatacc gccaacaccg aggatacgaa tcccggactc atcagacaca ccgccgccac
      301 cgtataacgg ataggccaca acatctgata aacggatacc ctcccgccgc ttctcattgc
      361 cgcgcccgcc aataaaagac cggacagccc cgtatgcctg agcatcagca cgccggcggc
      421 aaaggttgcc gccattagaa acagcgtcca accgccgccc aaccaatcgg caacccacac
      481 aatcgacata atctccaaaa acagcagcac caaaaaaccg ataccgaaaa atctcattga
      541 ccgtcatcct tatatttaag taaacagcaa accgcccgaa caggactcca agcgagctgc
      601 ctgtaaatga ttacaaaacc atgtgcttca agccgaaaca atgtgaaatc acgc
//
LOCUS       DAXIEB010000222          649 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000222 DAXIEB010000000
VERSION     DAXIEB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.222"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            144..530
                     /locus_tag="ACFOA3_002102"
     CDS             144..530
                     /locus_tag="ACFOA3_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6899030.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
BASE COUNT          242 a          100 c          126 g          181 t
ORIGIN      
        1 ccttggaaag gaacgaccat cattttaagg aatttttcaa ctagccgaga acaaactgga
       61 gcaaaatgga cgattgagtt tcaaaatcat ccattagtag gtaggaaaaa aatggaatta
      121 aaatttagat aaggaattga ataatgaaag gtttagataa gtatatccta tttaaaaatt
      181 gtgaaacatt aggagaattg tttcgaatat atcaagacta tctatcatta aatattgatg
      241 acccaaattt aaaaattaaa ttttttgacc ttttggaaga acttttatca gatggtgtta
      301 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aaccggttcg ccaaaaacac
      361 aggtagatga attaagaaaa atttggccgg atatggaaga gatgttgcta tattttccag
      421 ataacccttg gcattatgtt gaatggtttt ggtggggagc aacttgccct attgaactga
      481 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaagcttaa agccagcgta
      541 accagcctgt atgacaaggt gcaggtgaac aatccaatat ccacattacc ggctcagatg
      601 ttgccggcaa agaaggaacc ggcctgattg cagataagca aatccatct
//
LOCUS       DAXIEB010000223          627 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000223 DAXIEB010000000
VERSION     DAXIEB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.223"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..241
                     /locus_tag="ACFOA3_002103"
     CDS             <1..241
                     /locus_tag="ACFOA3_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6899031.1"
                     /translation="KLDVSGFIYYRINHSKEFADRQNHINGIENFWNQAKRVLRKYNG
                     IDRKSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT          147 a          137 c          164 g          179 t
ORIGIN      
        1 caagttggac gtgagcggtt tcatttatta ccgcatcaac cattccaagg aatttgcaga
       61 ccgtcaaaac cacattaacg gcattgagaa cttttggaat caggcaaaac gtgtcttgcg
      121 aaaatacaat ggaatcgatc gcaaatcttt cccgctgttc ttgaaagaat gcgaatttcg
      181 atttaacttc ggcacaccgt ctcaacagct taaaatcctg tgggatttag ggctaatcta
      241 gtacagcccc ttagatttaa ttcactatac ctgcgtaatg gtatcgggta ctcatcatca
      301 acctgccttg cattcggttg tgttgtccgg caactgtccg gttgtgtcga tgggttgccc
      361 tgccgctccc tgtgctgcgc aacggttgtg tgccttgggt gggcgcgggt ggcggccggg
      421 cggtttgttg ctatgatacg ggagttccga tatacaaggg tgggcttcag cccaccaatc
      481 aacgaccgaa aaagaaaaaa cgttgggctg aagcccactc tacgcaatta cgcaactgtt
      541 atcgtgggga tgacgggatt ttaggttttt gtttttctgt ttttgcggga aggatgggtt
      601 ttaggttgcg gtcatttatc ggaaaag
//
LOCUS       DAXIEB010000224          607 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000224 DAXIEB010000000
VERSION     DAXIEB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.224"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            42..260
                     /locus_tag="ACFOA3_002104"
     CDS             42..260
                     /locus_tag="ACFOA3_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6899032.1"
                     /translation="MKFINTCRKYGAKLAVVTAVPLALATQANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            267..545
                     /locus_tag="ACFOA3_002105"
     CDS             267..545
                     /locus_tag="ACFOA3_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899033.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGVMASVYFVSLLKKVLK"
BASE COUNT          165 a          126 c          155 g          161 t
ORIGIN      
        1 ctccatccat taccgccaaa cactttttga aggaaaatat catgaaattt attaacacct
       61 gccgcaaata cggcgcaaaa ctggctgttg taacagctgt cccgctggct ttggcgacac
      121 aggcaaatgc aacgttgccc gatacggcaa aaaacgcttt ggaagccgca aaagcggacg
      181 gtatggaagc cggttggatt gtagtgggcg ttttcgccgc gctttttgtc ttttccatcg
      241 ttaagagagt gatgaagtaa gacggcatgt actaccaagt cggaaataaa tgtcttgaga
      301 aacaccaggc tgaaaacctt tatttcagct tggtagtacc aagaatcaaa gaaaacggac
      361 agattgtcag gccggaatat aacggcagcc tgtggaagat gtcggacggt cagccgctaa
      421 ggcttttatt ggcggaatgc agtccgaaag acaacctgca aagcggtctt gaaacaggct
      481 ggatagtatt cggcgtgatg gcgtccgttt actttgtttc cctgctgaaa aaggttttga
      541 aatgatggat ttttattttt atctcggcgt ttccgtaccc gtattaatcg gggcggtttt
      601 gtttaag
//
LOCUS       DAXIEB010000225          607 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000225 DAXIEB010000000
VERSION     DAXIEB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.225"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            42..260
                     /locus_tag="ACFOA3_002106"
     CDS             42..260
                     /locus_tag="ACFOA3_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6899034.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            267..545
                     /locus_tag="ACFOA3_002107"
     CDS             267..545
                     /locus_tag="ACFOA3_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899035.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          164 a          130 c          150 g          163 t
ORIGIN      
        1 ctccatccat taccgccaaa cactttttga aggaaaatat catgaaattt attaacacct
       61 gccgtaaata cggtgcaaaa ctggctgttg taacagctgc tcccctggct ttggccgcac
      121 atgcaaatgc aacgttgccc gatacggcaa aaaacgcttt ggaagccgca aaagccgacg
      181 gtatggaagc cggttggatt gtagtgggcg ttttcgccgc gctttttgta ttttccatcg
      241 ttaagagagt gatgaagtaa gacggcatgt actaccaagt cggaaataaa tgtcttgaga
      301 agcaccaggc tgaaaacctt tatttcagct tggtagtacc aagaatcaaa gaaaacggac
      361 agattgtcag gccggaatat aacggcagcc tgtggaagat gtcggacggt cagccgctaa
      421 ggcttttatt ggcggaatgc agtccgaaag acaacctgca aagcggtctt gaaacaggct
      481 ggatagtatt cggcatcctc gcgtccgttt actttgtttc cctgctgaaa aaggttttga
      541 aatgatggat ttttattttt atctcggcgt ttccgtaccc gtattaatcg gggcggttct
      601 gtttaag
//
LOCUS       DAXIEB010000226          589 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000226 DAXIEB010000000
VERSION     DAXIEB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.226"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          137 a          187 c          138 g          127 t
ORIGIN      
        1 aatctcgaaa ttcgtcattc ccgcgcaggc gggaatccag ccccctgacg cggcgggaat
       61 ctatcggaaa tgactgaacc cccgagattc tagattccca cttccgtggg aatgacgtgg
      121 tgcaggtttc cgtatggatg gattcgtcat tcccgacaac accgcaatct cgaaacccgt
      181 cattcccgcg caggcgggaa tccagccccc tgacgcggcg ggaatctatc ggaaatgact
      241 gaaacccccg agattctaga ttcccacttt cgtgggaatg acggttcagt tgcgctccga
      301 caacaccgta atctcaaaac ccgtccgaca acaccgcaat cttgaaattc gtcattcccg
      361 cacaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac agttgcccaa
      421 accccgcgtt ctagattccc actttcgtgg gaatggcggt tcagttgcta cggccccgcc
      481 ctacaatacg ccttgcgaat ctgtcccacg cccgcccttg tatgcctcaa atgggttaaa
      541 ctacgcactt cccgacttcc cgtttcgggc aggggtacag gtccgattt
//
LOCUS       DAXIEB010000227          579 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000227 DAXIEB010000000
VERSION     DAXIEB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.227"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..268
                     /locus_tag="ACFOA3_002108"
     CDS             <1..268
                     /locus_tag="ACFOA3_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6899036.1"
                     /translation="ETKFKSRCFGGKSAVRKALYMATVAATRFEPLIRDFYQRLLSEG
                     KPYKVAVTACMRKLLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          136 a          132 c          160 g          151 t
ORIGIN      
        1 ggaaaccaaa ttcaaaagcc gctgctttgg cggaaagtct gcggtacgta aggcgctgta
       61 tatggctacc gtggcagcga cacgttttga accgcttatt cgggatttct accaacgcct
      121 gctgtccgag ggtaagccgt ataaggttgc cgttacggca tgtatgcgca aactgctgac
      181 gatatcgaat gcccggatgc gtgattattt tgccgaaaac gataccgccg aaaacgatac
      241 cgccgaaaac ggtatccgga cggcttgatt tgagttttgg tatttttgcc cgacggggtg
      301 aaaaatacag ttgctacggc tcgatgaatc gtcagaaata cccgaaccgt cattcccgcg
      361 caggcgggaa tctagaccgg tcggtgcgga aacttatcgg gaaaaaaggt ttctccgtcc
      421 ctgaatcctg gattcccact ttcgtgggaa tgacagaatg taggttcgtg ggaatgacgt
      481 ggtgcaggtt tccgtgcgga tgaattcatc attcccgcgc aggcgggaat ctagtctgtt
      541 cggtttcagt tattttcgat aaatgcttgt tgcttttca
//
LOCUS       DAXIEB010000228          560 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000228 DAXIEB010000000
VERSION     DAXIEB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.228"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..>560)
                     /gene="mafB"
                     /locus_tag="ACFOA3_002109"
     CDS             complement(<1..>560)
                     /gene="mafB"
                     /locus_tag="ACFOA3_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6899037.1"
                     /translation="ETHFSGHGHEVHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGS
                     EIHPEDGYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGA
                     ASGFFSRADEAGKLIWESDPNKNWWANRMDDIRGIIQGAANPFLTGFQGLGVGAITDS
                     AVNPVTYAAARKTLQGIHNLGNLSPEA"
BASE COUNT          130 a          142 c          123 g          165 t
ORIGIN      
        1 gcttccggac ttaaatttcc taaattgtga ataccctgta aagttttccg tgctgccgca
       61 taggttaccg ggtttaccgc actgtctgta attgccccaa ctcccaatcc ctgaaaaccc
      121 gttagaaaag gattggctgc accttggatg atgccgcgaa tatcatccat acggttagcc
      181 caccaatttt tattggggtc gctttcccat atcagttttc ctgcttcatc cgcacggctg
      241 aaaaaaccag aggcggcacc ggcattttct tttagccagc ggtctgaaaa tggggcttga
      301 gggacaatat tagtctttgt ttttgttgaa gttcctttga cataatagct gtatatatcc
      361 cttgctcctc cggggggcgg ataatcgctg ccttgcggtc cgtcatatcc atcctccgga
      421 tgaatttccg accctgttcg atgaagttgg taaacagtaa aaccgccgtc tacaccgccg
      481 ctgaaatcag aagtgcttct tgaatcatga ttatcgaacg gactgtgcac ttcatgtcca
      541 tgtcctgaaa aatgggtttc
//
LOCUS       DAXIEB010000229          555 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000229 DAXIEB010000000
VERSION     DAXIEB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.229"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..431
                     /locus_tag="ACFOA3_002110"
     CDS             <1..431
                     /locus_tag="ACFOA3_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6899038.1"
                     /translation="RQKILTDDAVRLALRQAVMAVRERYPFEILAWVLMPDHLHTIWR
                     LPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEYTIRDEADFAWHFDYLHFNPVK
                     HGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSIGYD"
BASE COUNT          139 a          121 c          151 g          144 t
ORIGIN      
        1 aacggcagaa gattttgacc gatgatgcgg tgcgtttggc tttacggcag gcggtaatgg
       61 cggtgcgcga acggtatccg tttgaaattt tggcatgggt gttgatgccc gatcatctgc
      121 ataccatatg gcggctgccg gacaatgatt ctgcttattc ggaacgctgg cggcaaatca
      181 agcggcacag ccaatattta atcggcggca atctcaggct ttggcaaaaa cgcttttggg
      241 aatatactat ccgcgatgag gccgattttg cctggcattt tgattatctg catttcaatc
      301 cggtcaaaca tggctatgta ggacaaattt ccgattgggg gttttctacg tttcaccgtt
      361 atgtcaaaca gggtatttat ccgcataatt ggggtggcgg caatgcggac ttttctattg
      421 gatacgattg aagtaatgtc ggattcgaga atccgaccta cggaaaactg aaagagcatc
      481 ggctgcagga cggcattatg cgcaaagcct gccgcaaccg tccgctgacg gaaaatcaaa
      541 ccaagcgtaa ccgat
//
LOCUS       DAXIEB010000230          553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000230 DAXIEB010000000
VERSION     DAXIEB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.230"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..>553)
                     /locus_tag="ACFOA3_002111"
     CDS             complement(<1..>553)
                     /locus_tag="ACFOA3_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899039.1"
                     /translation="RGDTYGGWRYLANLGDRYADDAAAIVGKDTNLNGLNLWMKKGVE
                     NLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTENGVS
                     SSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNGSEWDNRKQLISALDK
                     GFDGSFKEKPFTFLQSVIMDVTKL"
BASE COUNT          167 a          136 c           76 g          174 t
ORIGIN      
        1 taactttgtt acatccatta tcacagattg taaaaaagta aaaggcttct ctttaaaaga
       61 tccatcaaat cctttatcta aagcagatat taactgcttt ctattatccc attcgctacc
      121 gttcggatta tttactgctt gctcattgtg gatacgttcg gcttctatcc ccaaacctaa
      181 tgcccaataa ccatccgcca tatccggaag tgaacgatta ataactaaat caatagctgc
      241 actagaagaa acaccatttt cggtaacggc tttatagtaa cttctctcaa tttcactagt
      301 gttaggtaat ctaccattat tttcattaat tagatctaca tattggctga aatgttgcag
      361 tgcaacccga tcaaatttct ctaaacgggt ctttttaccg accgtatcat cccataggtt
      421 ttccacacct tttttcatcc ataaattcaa accatttaag tttgtatcct taccgacaat
      481 tgcggcagca tcatccgcat aacggtcacc caaattagcc aaataacgcc aaccgccata
      541 ggtatcgcca cgg
//
LOCUS       DAXIEB010000231          553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000231 DAXIEB010000000
VERSION     DAXIEB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.231"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..>553)
                     /locus_tag="ACFOA3_002112"
     CDS             complement(<1..>553)
                     /locus_tag="ACFOA3_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899040.1"
                     /translation="RGDTYGGWRYLANLGDRYADNAAAIVGKDANLNGLNLWMKKGVE
                     NLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTYHGVS
                     SSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNGSESDNRKQLISALDK
                     GFDGSFKEKPFTFLQSVIMDVTKL"
BASE COUNT          169 a          135 c           77 g          172 t
ORIGIN      
        1 taactttgtt acatccatta tcacagattg taaaaaagta aaaggcttct ctttaaaaga
       61 tccatcaaat cctttatcta aagcagatat taactgcttt ctattatccg attcgctacc
      121 gttcggatta tttactgctt gctcattgtg gatacgttcg gcttctatcc ccaaacctaa
      181 tgcccaataa ccatccgcca tatccggaag tgaacgatta ataactaaat caatagctgc
      241 actagaagaa acgccatgat aggtaacggc tttatagtaa cttctctcaa tttcactagt
      301 gttaggtaat ctaccattat tttcattaat tagatctaca tattggctga aatgttgcaa
      361 tgcaacccga tcaaatttct ctaaacgggt ctttttaccg accgtatcat cccataggtt
      421 ttccacccct tttttcatcc ataaattcaa accatttaag tttgcatcct taccgacaat
      481 tgcggcagca ttatccgcat aacggtcacc caaattagcc aaataacgcc aaccgccata
      541 ggtatcgcca cga
//
LOCUS       DAXIEB010000232          545 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000232 DAXIEB010000000
VERSION     DAXIEB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.232"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            1..262
                     /locus_tag="ACFOA3_002113"
                     /pseudo
     CDS             1..262
                     /locus_tag="ACFOA3_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            337..>545
                     /locus_tag="ACFOA3_002114"
     CDS             337..>545
                     /locus_tag="ACFOA3_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HFC6899041.1"
                     /translation="MGRKQPKTCVGVSAVGRERNFAKVSSYAGCCRPLHLRQILPPTA
                     EALMRSRYSAYVLHLIDYSGLTKTST"
BASE COUNT          136 a          138 c          154 g          117 t
ORIGIN      
        1 aagaacatca gttgttggac ggcattatgc gcaaagcctg ccgcaaccgt ccgctgacgg
       61 aaacgcaaac caaacgcaac cggtatttat cgaagacccg ttatgtggtc gaacaaagct
      121 tcggtacgct gcaccgtaaa ttccgctacg ctcgggcagc ctatttcgga ctgattaagt
      181 gagtgcgcaa agccatctga aggcgatgtg tttgaacctg ttgaaagccg ccaacaggct
      241 aagtgcgcct gttgccgcct aaaaggcagc acggatgcct gattatcggg tatccgggga
      301 ggattaaggg ggcgtttggg tagaattagg agatatttgg ggcgaaaaca gccgaaaacc
      361 tgtgttgggg tttcggctgt cgggagggaa aggaattttg caaaggtctc aagctatgcc
      421 ggctgctgcc gcccacttca tctgcgccaa atcctgccgc ccaccgccga agccctcatg
      481 cgttcgcggt acagcgcgta tgtgctgcac cttatcgact atagtggatt aacaaaaacc
      541 agtac
//
LOCUS       DAXIEB010000233          536 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000233 DAXIEB010000000
VERSION     DAXIEB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.233"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..260
                     /locus_tag="ACFOA3_002115"
     CDS             <1..260
                     /locus_tag="ACFOA3_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6899042.1"
                     /translation="KETTTVFSKSQGSTAPGKIPGSVTKPPYHESNSIGSLGLGVISG
                     VGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYHF"
BASE COUNT          134 a          163 c          126 g          113 t
ORIGIN      
        1 gaaaagaaac cacgactgtt ttcagtaaat cacaaggctc tacagcgcca ggcaagatcc
       61 caggttcggt taccaaacct ccctatcacg aaagcaacag catcggcagc ttggggcttg
      121 gtgtcatctc cggtgtcggt ttcgacatca cacccaagct gaccttggac accggatacc
      181 gttaccacaa ctggggacgc ttgaaaaaca cccgcttcaa aacccacgaa gtctcattgg
      241 gcatgcgcta ccacttctga ttccccgaca ccgatgccgt ctgaaccttc agacggcatt
      301 tttgatgcac ctaccgttta cagacgcggg gcgggcgtgg aaatacccga accgtcattc
      361 ccgacaacac cgtaatctcg aaatttgtca ttcccgcaca ggcgggaatc cagccccccg
      421 acgcggcggg aatctatcgg aaatgactga aatcccgcgt tctagattcc cacttccgtg
      481 ggaatgacgt ggtgcaggtt tccgtatgga tggattcgtc attcccgaca ataccg
//
LOCUS       DAXIEB010000234          524 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000234 DAXIEB010000000
VERSION     DAXIEB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.234"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..237)
                     /locus_tag="ACFOA3_002116"
     CDS             complement(<1..237)
                     /locus_tag="ACFOA3_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC6899043.1"
                     /translation="MFRILIISFLFLLSAKSFSADLEIKNGKLMYALSEKYNDNGFKA
                     YKVLGEGGGIHTEYNYKFDKSLNLNVLESSTGARS"
     gene            complement(191..481)
                     /locus_tag="ACFOA3_002117"
     CDS             complement(191..481)
                     /locus_tag="ACFOA3_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899044.1"
                     /translation="MNKNERDFFYISNSDLDKLSESYPDRPLSYVFYCYLKETGLLKN
                     FSMDKCHNFFNRINFNESCFEIKFKDDSFFIIGNGKIDVSDSNNFFSVSFEC"
BASE COUNT          199 a           88 c           56 g          181 t
ORIGIN      
        1 agagcgtgcg cctgttgaac tttctaatac attcaaattc aaacttttat caaatttgta
       61 attatattct gtatgaattc ctcctccctc acctaaaact ttgtatgcct taaatccatt
      121 atcgttatat ttttccgaaa gtgcatacat caatttccca tttttaattt ctaaatctgc
      181 tgaaaaagat ttagcactca aaagaaacag aaaagaaatt attagaatcc gaaacatcaa
      241 tttttccatt gccaataatg aaaaatgaat catccttgaa tttaatttca aagcaagatt
      301 cattaaaatt aattctatta aaaaaattat gacatttatc cattgagaaa tttttcaata
      361 gaccagtttc tttcaaataa caataaaaca cataagaaag aggcctatca ggataagatt
      421 ctgacaattt atctaaatca gaatttgata tatagaaaaa gtcacgttca tttttattca
      481 tggtttcaag cctcaaatgt ctaattaaaa gcctgatttt gaca
//
LOCUS       DAXIEB010000235          481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000235 DAXIEB010000000
VERSION     DAXIEB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.235"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(279..>481)
                     /locus_tag="ACFOA3_002118"
     CDS             complement(279..>481)
                     /locus_tag="ACFOA3_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6899045.1"
                     /translation="NELRTLEELGIQSLDFAYKDVNKNLGKESKPQSAVLAIHTPFVC
                     FLGNLKGIICTRHNPILYFTKN"
BASE COUNT          174 a           73 c           77 g          157 t
ORIGIN      
        1 aaaataatgt aataaagtat attttttggc tatgaaataa aattgtacat aattgaacga
       61 gcagatcaaa aaatgaacta catataacaa taaataataa cgtatttacc atactaaatt
      121 taataggtct cattatcata tttaataacc acttcatagt aaatgcagag tgggtggaaa
      181 cactcacttt atggtttgct acgctctgct caattagcaa cccgataacc caatatagat
      241 aatagggcaa ttaatccaat ctaatttgtt aaaatacgtt aattttttgt aaaataaagt
      301 attggattat gtcgggtgca aatgatgcct tttagattgc ctaaaaaaca aacaaacggt
      361 gtgtgtatag caagtactgc actttgcggc ttactttctt taccgagatt tttatttaca
      421 tctttatagg cgaaatccaa agattgaata cccaattctt caagggtgcg gagttcgttg
      481 g
//
LOCUS       DAXIEB010000236          476 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000236 DAXIEB010000000
VERSION     DAXIEB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.236"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          144 a          112 c          107 g          113 t
ORIGIN      
        1 atggatggat tcgtcattcc cgcgcaggcg ggaatccaga cattcaatgc taaggcaatt
       61 tatcgggaat gactgaaact caaaaaactg gattcccact ttcgtgggaa tgacgcgaac
      121 agaaacctca aatcccgtca ttcccgcgca ggcgggaatc tagacattca atgctgaggc
      181 aatttatcgg aaatgactga aactcaaaaa actggattcc cactttcgtg ggaatgacgg
      241 cagagcggct tctgttgctc ccgataaatg ccgcaatctc aaatcccgtc attcccgcgc
      301 aggcgggaat ctagacattc aatgctaagg caatttatcg gaaatgactg aaactcaaaa
      361 aaactagatt cccacttttg tgggaatgac gcgattagag tttcaaaatt tattctaaat
      421 agctgaaact caacgcactg gattcccgcc tgcgcgggaa tgacgaattt caggtt
//
LOCUS       DAXIEB010000237          471 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000237 DAXIEB010000000
VERSION     DAXIEB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.237"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..203
                     /locus_tag="ACFOA3_002119"
     CDS             <1..203
                     /locus_tag="ACFOA3_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899046.1"
                     /translation="AYDCAFLKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHV
                     NNEESYNNFIYDKFARLPISWA"
     gene            248..>471
                     /locus_tag="ACFOA3_002120"
     CDS             248..>471
                     /locus_tag="ACFOA3_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899047.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNV"
BASE COUNT          162 a          124 c           86 g           99 t
ORIGIN      
        1 aagcctacga ctgcgccttt ttgaaagctc aagccattca tgaacagccc gcattcaaac
       61 cgtacaaaga cccttactat atgtacgaat attacgagaa tcttgaaaaa cagcttgaac
      121 agcaaaaaca cgtcaacaat gaagaaagct ataacaactt catttacgac aaattcgcaa
      181 gactaccgat ttcatgggct taaagtgtct gcccgaaaga cgtttaatca cacaaggaaa
      241 ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc gttaaaaaat tcaacggaca
      301 aatcgaaggc aagagcttcg actattgccg cctgattgtc gccacaccct tagacagctc
      361 ccaaggcaac gcattgggca gctctactac tgaatacgat ttcggcggct ctgccaattt
      421 cgagcagttc cgaaacgccc aatttccgat cgaagcaaac ctgaacgtag a
//
LOCUS       DAXIEB010000238          470 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000238 DAXIEB010000000
VERSION     DAXIEB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.238"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>470
                     /locus_tag="ACFOA3_002121"
     CDS             <1..>470
                     /locus_tag="ACFOA3_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6899048.1"
                     /translation="IQSVQDSETYQSKQQNASAQVTVGYGFSASGDYSQSKIRANHAS
                     VTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTQSAEDKGKNRFSTGTLAGSDIQNYSQ
                     YEGKSFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQ"
BASE COUNT          170 a          118 c          113 g           69 t
ORIGIN      
        1 attcaaagcg tacaagatag cgaaacctat caaagcaaac aacaaaacgc cagtgcacaa
       61 gtgaccgtcg gttatggctt cagtgccagt ggcgattaca gccaaagcaa aatccgagcc
      121 aatcatgctt cggtaaccga gcaaagcggt atttatgccg gagaagacgg ctatcaaatc
      181 aaggtcggaa accatacaga cctcaaaggc ggcatcatca ccagtaccca aagcgcagaa
      241 gacaagggta aaaaccgatt cagcacaggc acactcgccg gcagtgatat tcaaaattac
      301 agccaatacg aaggaaaaag ttttggattg ggcgcaagtg cgtccataag cggcaaaaca
      361 ctgggacagg gcgcacaaaa taagcctcaa aacaaacacc tgacaagcgt ggccgataaa
      421 aacggcgcaa gttcatcagt aggctacggc agcgacagcg acagccaaag
//
LOCUS       DAXIEB010000239          461 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000239 DAXIEB010000000
VERSION     DAXIEB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.239"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          138 a          105 c          119 g           99 t
ORIGIN      
        1 ctgaaggcga tgtgtttgaa cctgttgaaa gccgccaaca agctaagtgc gcccgctgcc
       61 gcctaaaagg agaccggatg cctgattatc gggtatccgg ggagggttaa gggggtattt
      121 gggtaaaatt aggaggtatt tggggcgaaa atagacgaaa acctgtgttt gggtttcggc
      181 tgtcgggagg gaaaggaatt ttgcaaagat ctcatcctgt tattttcaca aaaacagaaa
      241 accaaaaaca gcaacctgaa attcgtcatt cccgcgcagg cgggaatcca gacccccaac
      301 gcggcaggaa tctatcggaa ataaccgaaa ccggacgaac ctagattccc gctttcgcgg
      361 gaatgacggc agagtggttt cagttgctcc cgataaatgc cgccatctca agtctcgtca
      421 ttcccttaaa acagaaaacc gaaatcagaa acctaaaatt t
//
LOCUS       DAXIEB010000240          452 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000240 DAXIEB010000000
VERSION     DAXIEB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.240"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>452
                     /locus_tag="ACFOA3_002122"
     CDS             <1..>452
                     /locus_tag="ACFOA3_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899049.1"
                     /translation="GQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFR
                     VLSGGKYGGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQ
                     VLDQLPDGSPAKAAVFKANKNGKLKTAIAGVDRQTGKAVILSVKVPSK"
BASE COUNT          142 a           79 c          119 g          112 t
ORIGIN      
        1 tcggtcaaac tgaattaagg gttactgcag caactgacaa acaattgctg aaagctattg
       61 gcgaaggaag ggacacgaca ggtaaaatga ccgagcagtt atttgactct ttagctaaac
      121 aaaatggctt cagagtgctt tcgggcggca aatacggcgg aaataacggt tttgatcatg
      181 tatggcaggc tgccgatggt agtgtcgttt tgattgtaga aagtaagcag attaggaacg
      241 gtacggtaca gctgaatccg aatggtgcgg gtggatatac gcagatgagt cgtgaatgga
      301 ttaaacaagt tttagatcaa ttacccgatg gtagtcccgc taaggcagct gtcttcaaag
      361 caaataagaa cggcaaatta aaaacggcaa tagcaggcgt tgatcgtcaa acaggtaagg
      421 ccgttattct ttctgtcaaa gttccttcta aa
//
LOCUS       DAXIEB010000241          437 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000241 DAXIEB010000000
VERSION     DAXIEB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.241"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            24..407
                     /locus_tag="ACFOA3_002123"
     CDS             24..407
                     /locus_tag="ACFOA3_002123"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6899050.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
BASE COUNT           93 a          116 c          119 g          109 t
ORIGIN      
        1 gagaacattt tgaaaggata ccgatggaac cttcctccta cgcggcagaa aaaaaaggca
       61 aaggcggcat caggcgcgtc attaacgcat tcggctattc gatagacggc atcgccgccg
      121 cctaccgtta cgaagcggca ttccgtcagg ttttgtggct gaacgtactg ctggtgtgcg
      181 cggcattttt ttgggtttcc gaaaccgccg tccgcctgcc gttgattatc gcgtcttttg
      241 tgtcggtcat tgtcgaactg ttcaacaccg ccgtcgaagc tgccgtcgat catacttcga
      301 ccgaaaaaca cgagctggcc aaacgcgcca aagacgcagg ttctgccgca caattgttcg
      361 cgatgctgat gttggcggcg gtttggctgt ctgccctgtt cgggtaaaac gcttgcagca
      421 ggatttgtaa tccttta
//
LOCUS       DAXIEB010000242          431 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000242 DAXIEB010000000
VERSION     DAXIEB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.242"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..>431)
                     /locus_tag="ACFOA3_002124"
     CDS             complement(<1..>431)
                     /locus_tag="ACFOA3_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6899051.1"
                     /translation="LIFVNPLYITDKVVALLKANGVPAALVDMGEIRGFDTNGKRTWN
                     VGIRNLDDEEGVLANITMKDKAFATSGGYGTVMDKAGRFTHLFDPRTGAGTPRYKSMS
                     VMADDAAVADALSTAFSIMDLPLIRSVAESRRLKVWLVMPD"
BASE COUNT          104 a          139 c           94 g           94 t
ORIGIN      
        1 gtccggcatg accagccaaa ccttaagccg cctgctttcg gcaaccgacc gtatcaaggg
       61 caaatccata atggaaaacg ccgtcgacaa ggcatcggca accgccgcat catccgccat
      121 cacgctcata cttttataac gcggcgtacc cgcgccggtc cgcggatcga acaaatgggt
      181 aaacctgccc gccttgtcca tcaccgtccc atagccgccc gaagtggcaa acgccttgtc
      241 tttcatcgtg atatttgcca acacgccttc ttcgtcgtcc agattgcgga tgccgacatt
      301 ccaagtccgt ttaccgtttg tatcaaatcc cctgatttcg cccatatcga ccagcgcggc
      361 aggcacgccg tttgctttaa gcagcgcaac aaccttgtcc gtgatatata gtggattaac
      421 aaaaatcagg a
//
LOCUS       DAXIEB010000243          426 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000243 DAXIEB010000000
VERSION     DAXIEB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.243"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(221..>426)
                     /locus_tag="ACFOA3_002125"
     CDS             complement(221..>426)
                     /locus_tag="ACFOA3_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6899052.1"
                     /translation="TASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVYETF
                     KEDIQARGYQYDPETDKFAKVSG"
BASE COUNT          102 a          128 c           90 g          106 t
ORIGIN      
        1 tatttccctc aagcgatgcg gcgacaccaa cacctgatta aaacacaacc ggagaccttt
       61 gcaaaattcc tttccctccc gacagccgaa acccaaacac aggttttcgt ctatttccgc
      121 taccaatcac tccctaattc tacccaaata cccccttaat cctccccgga tacccgataa
      181 tcaggcatcc gggttacctt ttaggcggca acaggcgcac ttagcctgag acctttgcaa
      241 atttgtcggt ttcggggtcg tattggtagc ctcgtgcctg tatgtcttct ttgaaagttt
      301 cgtatacgtc atgggctaaa agggctgttc cgacataagg aactgccctt gtgctgaatt
      361 tcgcgcctaa gcgggcaagt ttgccaaccc ccgccaatac gccggcgcgg gaaactgatg
      421 cggtaa
//
LOCUS       DAXIEB010000244          424 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000244 DAXIEB010000000
VERSION     DAXIEB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.244"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          154 a           42 c           82 g          146 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccagtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 agactccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatc actttcctag agtaaggata
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgta
//
LOCUS       DAXIEB010000245          424 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000245 DAXIEB010000000
VERSION     DAXIEB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.245"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(12..224)
                     /locus_tag="ACFOA3_002126"
     CDS             complement(12..224)
                     /locus_tag="ACFOA3_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6899053.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT           87 a          116 c          114 g          107 t
ORIGIN      
        1 tttttgccgc tttatttcaa cgcggcatca tagccgccgt cttcaacggc ttcaatcaac
       61 gcccctgcat cggtttgcgc ggggtcgtat ccgacggtcg cgcttttgtt ttcaaggctg
      121 acttcgacgc ttgccacgcc tttgacgctt tccagtatcc gggtaacgct tttgacgcag
      181 ccgccgcagc tcatgccgcc gatgtcgagg ataagggttt ccatgatttt cctttcgttg
      241 gaactgcaat ctgacgagcg ttattgtaag tcggggcgtg aacttgggca aacgcggaaa
      301 cggtgcggcg gtttggaaaa agacggacgc ttgcgcataa tggcggcaat tcccatcagg
      361 acgacaacaa tgaacgcttc acaaaaaccc tggttgagca tcatcgcctt ggcaatcggc
      421 gcat
//
LOCUS       DAXIEB010000246          408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000246 DAXIEB010000000
VERSION     DAXIEB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.246"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..210
                     /locus_tag="ACFOA3_002127"
     CDS             <1..210
                     /locus_tag="ACFOA3_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6899054.1"
                     /translation="AAMLDKVEALLTVGGKHWNYAHAMARRMFGKDKVEYLDDTQLHK
                     LVAALQIAENRKTEKAGGDDGVRKS"
     gene            191..>408
                     /locus_tag="ACFOA3_002128"
     CDS             191..>408
                     /locus_tag="ACFOA3_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HFC6899055.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGE"
BASE COUNT          110 a           73 c          138 g           87 t
ORIGIN      
        1 gcggcaatgt tggacaaagt cgaagccctg ctgaccgtcg gcggcaaaca ttggaactat
       61 gcacacgcaa tggcgcggcg gatgtttggt aaggataagg tcgaatattt agacgatacg
      121 cagctacata aactggttgc tgcgttgcag attgcggaaa acaggaaaac ggaaaaagcg
      181 ggtggggatg atggcgttcg aaaaagttga acatttattg ccggataccg tgttggacat
      241 tgtggatgtc atcggactgg cagcgacgga acagctggtc aaggcgattg gcggggcgcg
      301 gtttaaattt ggtaagggca aggtggacac cgagcgtttg gcaattttgg tcgaagccat
      361 cggcgaagtg aaaacacatg agctgttgca ggtatatggt ggcgagga
//
LOCUS       DAXIEB010000247          402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000247 DAXIEB010000000
VERSION     DAXIEB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.247"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..202
                     /locus_tag="ACFOA3_002129"
     CDS             <1..202
                     /locus_tag="ACFOA3_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6899056.1"
                     /translation="YFAERQNHINGIENFWNRAKRHLRKFDGIPKAHFELYLKGYERR
                     FNNSEIKVQISILKQLVKSSLS"
     gene            282..402
                     /locus_tag="ACFOA3_002130"
                     /pseudo
     CDS             282..402
                     /locus_tag="ACFOA3_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          147 a           75 c           77 g          103 t
ORIGIN      
        1 ttattttgct gaacgacaaa accatatcaa tggaattgag aacttttgga accgggcaaa
       61 acgtcattta cgcaagtttg acggcattcc caaagcgcat tttgagctgt atttaaaggg
      121 gtacgaacgg cgttttaaca acagtgagat aaaagttcaa atttccattt taaaacaatt
      181 agtaaaatcg agtttatcct agttatctag gacagcccca aaaacaaaat agtacaatat
      241 tcaactttga aggtctaacc atggcatact ctgcggactt aagaaacaaa gctttaaact
      301 atagtggatt aaatttaaat caggacaagg cgacgaagcc gcagacagta caaatagtac
      361 ggcaaggcga ggcaacaccg tactggttta aatttaatcc ac
//
LOCUS       DAXIEB010000248          387 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000248 DAXIEB010000000
VERSION     DAXIEB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.248"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          116 a           94 c           78 g           99 t
ORIGIN      
        1 tcccgtcatt cccacgaaag tgggaatcta gaaatttaat gttgcggcac tagccaaaaa
       61 aaccgaaacc gaacggacta gattcccgcc tgcgcgggaa tgacggcgga gcggtttctg
      121 ttttttccgg taaataccca caagctaaaa tcccattatt ttcacaaaaa cagaaaacca
      181 aaaacagcaa cctgaaattc gtcattcccg ctcaggcggg aatccagtgc gttgagtttc
      241 agctatttag aataaatttt gaaactctaa tcgcgtcatt cccacaaaag tgggaatcca
      301 gttttttgag tttcagtcat ttccgataaa ttgccttagc gttgaatgtc tagattcccg
      361 cctgcgcggg aatgacgaat ccatcca
//
LOCUS       DAXIEB010000249          374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000249 DAXIEB010000000
VERSION     DAXIEB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.249"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           95 a           77 c          113 g           89 t
ORIGIN      
        1 gattttaggt tcgtgggaat gacggaatgt aggttcgcgg gaatgacgcg gtgcaggttt
       61 ccgtatggat gggttcgtca ttcccgcgca ggcgggaatc tagaacgtaa aatctaaaga
      121 aaccgtgttg taacggcaga ccgatgccgt cattcccgcg caggcgggaa tccagaccat
      181 tggacagcag caatattcaa agattatctg aaagtccggg attctagatt cccactttcg
      241 tgggaatgac gggatgtagg ttcgtgggaa tgacgcggtg caggtttccg tacggatgga
      301 ttcgtcattc ccgcgcaggc gggaatccag accttagaac aacagtaata ttcaaaggtt
      361 agctgaagct ttag
//
LOCUS       DAXIEB010000250          372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000250 DAXIEB010000000
VERSION     DAXIEB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.250"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>372
                     /locus_tag="ACFOA3_002131"
     CDS             <1..>372
                     /locus_tag="ACFOA3_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6899057.1"
                     /translation="QPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHQITLHHSTIY
                     RYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSRIGD
                     WEADTIVGKGQKSALLTLVERV"
BASE COUNT          113 a          122 c           77 g           60 t
ORIGIN      
        1 acaaccctat aagctcgatt cgcagctgat tcagcacatc gacaccctta tccgccgcaa
       61 actcagtccc gaacaagtat gcgcctacct gtgcaaacac caccagatca cgctccacca
      121 cagcaccatt taccgctacc ttcgccaaga caaaagcaac ggcagcacgt tgtggcaaca
      181 tctcagaata tgcagcaaac cctaccgcaa acgctacggc agcacatgga ccagaggcaa
      241 agtacccaac cgtgtcggca tagaaaaccg acccgctatc gtcgaccaga aatcccgtat
      301 cggcgattgg gaagccgaca ccattgtcgg caaaggacag aaaagcgcat tattgacctt
      361 ggtcgaacgc gt
//
LOCUS       DAXIEB010000251          372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000251 DAXIEB010000000
VERSION     DAXIEB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.251"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>372
                     /locus_tag="ACFOA3_002132"
     CDS             <1..>372
                     /locus_tag="ACFOA3_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6899058.1"
                     /translation="VVGGDVNTAANAAQIAVENNTLYPRCVGAKCDEFQKEQQKWIRE
                     NPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADHLFALLGVVPGIGESIQAYKVAKAA
                     KNLQGMKKALDKAATVATAQGY"
BASE COUNT          115 a           66 c          100 g           91 t
ORIGIN      
        1 gttgtaggcg gggatgtgaa tacagcggcg aatgcggcac agatagcggt ggagaataat
       61 actttgtatc ctagatgcgt tggtgcaaag tgtgatgaat ttcaaaagga acaacaaaaa
      121 tggatacgtg aaaatcctga agaatatcga gaagttttgc ttcttcagac aggatttatt
      181 ccaattatcg gtgatataca gagttttgta caagcacaga ccgctgccga tcacctgttt
      241 gctttgctgg gtgtggttcc gggtatcggt gaatcgatac aggcctataa agtagcgaaa
      301 gcggcaaaaa atttacaagg catgaaaaaa gccttggaca aggcagcaac cgttgccact
      361 gcacagggct at
//
LOCUS       DAXIEB010000252          370 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000252 DAXIEB010000000
VERSION     DAXIEB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.252"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          128 a           50 c           67 g          125 t
ORIGIN      
        1 tgtcaaagtg caggctgctt tgaaatcggt attgccatct atgaaccacc actttgcttt
       61 atttcagcgg gcttgagatg tgtataagaa tattgttttg aataaattta aagaaaatga
      121 taatcgttat tgacgatttt taaaggaaag cgtagagtgc caattctatg aagcaatacg
      181 gtaagtaaca atgaaaatat ctactgcttg ggtatagagc atatttcaca acccgtaact
      241 attcttgcgg aaacagagaa aaaagtttct cttctatctt ggataaatat atttaccctc
      301 agtttagtta agtattggaa tttataccta agtagtaaaa gttagtaaat tatttttaac
      361 taaagagtta
//
LOCUS       DAXIEB010000253          366 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000253 DAXIEB010000000
VERSION     DAXIEB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.253"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           97 a           86 c           95 g           88 t
ORIGIN      
        1 attgtttgcg ttttcgggat gggggcaaat gaaacgccga caaaccgata ccgtcattcc
       61 cgcgcaggcg ggaatctaga cattcaatgc taaggcaatt tatcagaaat gactgaaact
      121 caaaaaactg gattcccact ttcgtgggaa tgacgactcg gacattcctt aaactacccg
      181 tgtatcgctg taaatcttag agatggagga ataaagaccg ttgggcatct gcagccgtca
      241 ttcccgcgca ggcgggaatc taggatgcgg aatctcaaga aaccgttata cccgataagt
      301 ttctgtgccg acaggtctgg attcccgcct gcgcgggaat gacgattcgg gtatttctga
      361 cggttc
//
LOCUS       DAXIEB010000254          354 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000254 DAXIEB010000000
VERSION     DAXIEB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.254"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT          103 a           83 c           84 g           84 t
ORIGIN      
        1 cgggaactta tcggaaacga ccgaaaccga acggactgga ttcccgcttt tgcgggaatg
       61 acggcgacag ggttgctgtt atagtggatt aacaaaaacc ggtacggcgt tgtctcgcct
      121 tagctcaaag agaacgattc tctaaggtgc tgaagcacga gtgaatcggt tccgtaccat
      181 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atagaatttc
      241 aatgcctcaa gaatttatcg gaaaaaacca aaacccttcc gccgtcattc ccacgaaagt
      301 gggaatctag aaatgaaaag cagcaggaat ttatcggaaa cgaccgaaac cgaa
//
LOCUS       DAXIEB010000255          352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000255 DAXIEB010000000
VERSION     DAXIEB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.255"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           91 a           81 c           91 g           89 t
ORIGIN      
        1 ctgaagcacc aagtgaatcg gttccgtact atccgtactg tctgcggctc gccgccttgt
       61 cctgattttt gttaatctgc tataaagacc gtcgggcatc tgcagccgtc attcccgcgc
      121 aggcgggaat ctggaatttc aatgcctcaa gaatttatcg gaaaaaaaca aaacccttcc
      181 gccgtcattc ccacgaaagt gggaatctag aaatgaaaag cagcaggaat ttatcggaaa
      241 cgaccgaaac tgaacggact ggattcccgc ctgcgcggga atgacgggat tttaggtttc
      301 tgattttggt tttctgtcct tgtgggaatg acgaaaagtg gtgggaatga cg
//
LOCUS       DAXIEB010000256          350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000256 DAXIEB010000000
VERSION     DAXIEB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.256"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>350
                     /locus_tag="ACFOA3_002133"
     CDS             <1..>350
                     /locus_tag="ACFOA3_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899059.1"
                     /translation="YLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEML
                     AYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNV
                     GFGHNKNVSLYGNDG"
BASE COUNT          113 a           51 c           83 g          103 t
ORIGIN      
        1 tatttgaatc aaatcagttt caaaatggaa aatgatacgt tcactttgga ttttagtggt
       61 cttgttcaag catttaacca tgtcaaagaa actaatccgc aaaaagcttt tgtggatttg
      121 gccgagatgc ttgcatatgg cgaacttcgt tcttggtatg aaggccgaag actaatggcc
      181 gattatgtgg aggaggcaaa aaaagcaggt aaatttgaag attaccagaa agtgttgggt
      241 caggagaccg ttgcattatt agctaaaaca tcgggtacgc aagcagatga tatcctgcaa
      301 aatgtaggct ttggtcataa taaaaatgtt tctttatatg gtaatgacgg
//
LOCUS       DAXIEB010000257          337 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000257 DAXIEB010000000
VERSION     DAXIEB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.257"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           85 a           86 c           80 g           86 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aagaccgtcg ggcatctgca gccgtcattc
       61 ccgcgcaggc gggaatccag tccgcttgtt cggtaaatga gagggcggat tgcgcgcctg
      121 tcagataaac cccgtgttta aacgggcggc aatgaggtac gcgcagagcc ttgaagcgcg
      181 atcgatatat tatttccacc aaaacggacg cccccgcctg ccttgcaaac ctttaaaagg
      241 aagccacccg gattaatccg agtggccgtg gaaaatcact taccgcttga tttatttaaa
      301 atttatggta taatttacct tagctggcac tatgtaa
//
LOCUS       DAXIEB010000258          316 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000258 DAXIEB010000000
VERSION     DAXIEB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.258"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..215
                     /locus_tag="ACFOA3_002134"
     CDS             <1..215
                     /locus_tag="ACFOA3_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6899060.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
BASE COUNT          100 a           51 c           76 g           89 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaatt
//
LOCUS       DAXIEB010000259          305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000259 DAXIEB010000000
VERSION     DAXIEB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.259"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..206
                     /locus_tag="ACFOA3_002135"
     CDS             <1..206
                     /locus_tag="ACFOA3_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6899061.1"
                     /translation="TASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQYDPETEKFAKVSG"
BASE COUNT           83 a           72 c           83 g           67 t
ORIGIN      
        1 taactgcatc agtttcccgc gccgccgtct tgtcaggagt cggcaaactt gcccgcttag
       61 gcgcgaaatt aagcacaagg gcagttcctt atgtcggaac agccctttta gcccatgacg
      121 tatacgaaac tttcaaagaa gacatacagg cacaaggcta ccaatacgac cccgaaaccg
      181 agaaatttgc aaaggtctca ggctaagtgc gcctgttgcc gcctaaaagg taacccggat
      241 gcctgattat cgggtatccg gggaggatta aggggggatt tgggtaaaat taggaggtgt
      301 tatgg
//
LOCUS       DAXIEB010000260          302 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000260 DAXIEB010000000
VERSION     DAXIEB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.260"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           90 a           42 c           95 g           75 t
ORIGIN      
        1 tcagttgcta cggttattgt caggtttcgg ttatgttgga atttcgggaa acttatgaat
       61 tgtcattccc gcgcaggcgg gaatctagac tttagaacaa cagcaatatt caaagattat
      121 ctgaaagttt gagattctag attcccactt tcgtgggaat gacgggatgt aggttcgtgg
      181 gaatgacgaa aagtggcggg aatgacgaaa agttgcggga atgacgaaaa gttgcgggaa
      241 tgacgaaaag ttgcgggaat gacgaaaagt tgcgggaatg acggaaagtg gcgggaatga
      301 cg
//
LOCUS       DAXIEB010000261          293 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000261 DAXIEB010000000
VERSION     DAXIEB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.261"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           92 a          100 c           57 g           44 t
ORIGIN      
        1 cggcaggctg aagcccaccc taccattatt tacacatccg taccgcttaa atgccgtctg
       61 aaatttcgtc attcccatga aaacgggaat ccaaccccgt cggagcagaa acttacaccc
      121 cgtcattccc gcgaacgcgg gaatccagta accgaaaagc cacaggaatc tatcggaaaa
      181 aaacagaacc ccccgccgcc gtcattcccg cgaacgcggg aatccagtaa ccgaaaaacc
      241 acaggaatct atcggaaaaa acagaaaccc ccgccgccgt cattcccgcg aac
//
LOCUS       DAXIEB010000262          282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000262 DAXIEB010000000
VERSION     DAXIEB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.262"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
BASE COUNT           74 a           76 c           66 g           66 t
ORIGIN      
        1 tcccgtcatt cccacgaaag tgggaatcta gaacctctga attttcagac aacctttgaa
       61 tattgccgcc gtcccgcgtt ctagattccc gcctgcgcgg gaatgacgat tcatcagttt
      121 cccgaaacgc ccgaaaaacc gaaaccgagc aaaccggatt cccgcctgcg cgggaatgac
      181 gatttataag tttcccgaaa cgcccaaaaa aaccgaaatc cgacaggcgg gatttttgct
      241 ttcgtgggga tgacgggatt ttaagttacg gtcatttatc gg
//
LOCUS       DAXIEB010000263          276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000263 DAXIEB010000000
VERSION     DAXIEB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.263"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            1..276
                     /locus_tag="ACFOA3_002136"
                     /pseudo
     CDS             1..276
                     /locus_tag="ACFOA3_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           76 a           94 c           53 g           53 t
ORIGIN      
        1 tgtccatgtt caaagccgtt ctgctcggac aatggcacag cctctccgat cccgaactcg
       61 aacacagcct catcacccgc atcgacttca acctgttttg acgttttgac gaactgagca
      121 tccccgatta cagcacctta tgccgctacc gcaaccggcg acgcaagacg acaccctgtc
      181 cgaattgccg gaactgatca accgccaact gaccgaaaaa aacctaaaaa tagagaaagc
      241 atccgccgcc gtcgttgacg ccaccattat tcagac
//
LOCUS       DAXIEB010000264          252 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000264 DAXIEB010000000
VERSION     DAXIEB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.264"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            20..>252
                     /locus_tag="ACFOA3_002137"
     CDS             20..>252
                     /locus_tag="ACFOA3_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6899062.1"
                     /translation="MNLPIQKFMMLFAAAISLLQFPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILP"
BASE COUNT           67 a           61 c           61 g           63 t
ORIGIN      
        1 tgctaccaag gaaaacaaaa tgaatttgcc tattcaaaaa ttcatgatgc tgtttgcagc
       61 ggcaatatcg ttgctgcaat tccccattag tcatgcgaac ggtttggatg cccgtttgcg
      121 cgatgatatg caggcaaaac actacgaacc gggtggcaaa taccatctgt tcggcgatgg
      181 tcgcggcagt gttaaaaatc gggtttacgc cgtccaaaca tttgatgcaa ctgcggtcgg
      241 ccccatactg cc
//
LOCUS       DAXIEB010000265          236 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000265 DAXIEB010000000
VERSION     DAXIEB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.265"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>236
                     /locus_tag="ACFOA3_002138"
     CDS             <1..>236
                     /locus_tag="ACFOA3_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6899063.1"
                     /translation="TERSQNKSAGWNAGAAVSFGQGGWSLGVTAGGNIGKGHGNGDSI
                     THRHSHIGDKGSQTLIQSGGDTTIKGAQVRGKGV"
BASE COUNT           70 a           69 c           66 g           31 t
ORIGIN      
        1 accgaacgca gccaaaacaa atcggcaggc tggaacgcag gtgctgccgt atcattcgga
       61 caaggaggct ggtcattagg cgttaccgca ggcggcaata tcggcaaagg ccacggcaat
      121 ggcgacagca tcacccaccg ccatagccat atcggcgaca aaggcagcca aacccttatc
      181 caaagtggtg gcgatactac catcaaaggc gcgcaagtac gcggcaaagg cgtaca
//
LOCUS       DAXIEB010000266          236 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000266 DAXIEB010000000
VERSION     DAXIEB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.266"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>236
                     /locus_tag="ACFOA3_002139"
     CDS             <1..>236
                     /locus_tag="ACFOA3_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6899064.1"
                     /translation="EKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHFIGGDINKKG
                     TVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEI"
BASE COUNT           89 a           34 c           63 g           50 t
ORIGIN      
        1 gagaaaaatg ttcaggaaac gagaagaagg agtcagagta gtcagtttaa agcccatgcg
       61 caacgagaat gggaaaataa aacagggtta gattttaatc attttatagg tggtgatatc
      121 aataagaaag gcacagtaac aggagggcat agtctaaccc gtggtgatgt acgggtgata
      181 caacaaacct cggcacctga taaacatggg gtttatcaag cgacagtgga aattaa
//
LOCUS       DAXIEB010000267          228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000267 DAXIEB010000000
VERSION     DAXIEB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.267"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            complement(<1..>228)
                     /locus_tag="ACFOA3_002140"
     CDS             complement(<1..>228)
                     /locus_tag="ACFOA3_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6899065.1"
                     /translation="MKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDE
                     DPEPFFFPCDMLNIQDPMFLMLMSDSPQLREF"
BASE COUNT           68 a           49 c           50 g           61 t
ORIGIN      
        1 aaactcgcgc agctgcgggc tgtcgctcat cagcatcaga aacatcggat cttgaatgtt
       61 gagcatgtcg cagggaaaga agaagggttc ggggtcttca tcatctacac tctgcaaaat
      121 acccaacttt cctgcaacat aagcatattg cttgaatttc ttaagatcat gttcaaatag
      181 ataggcatgg gaagccgcag cttcagtgta cattaaaatg tatttcat
//
LOCUS       DAXIEB010000268          228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000268 DAXIEB010000000
VERSION     DAXIEB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.268"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>228
                     /locus_tag="ACFOA3_002141"
     CDS             <1..>228
                     /locus_tag="ACFOA3_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6899066.1"
                     /translation="ANDTEAFVNRYDLNRHMIYNTLLMVEGKQLDRLKQRSEKVLAHP
                     TPSKWLQKRLYDYRFFLAFAEQDAEAMKAAL"
BASE COUNT           60 a           61 c           57 g           50 t
ORIGIN      
        1 tcgccaacga tacagaagcc ttcgtaaacc gatacgacct caaccgtcat atgatttaca
       61 atactctgct gatggtggag ggtaagcagc ttgatcggtt gaaacaacgt agcgagaaag
      121 tcttggcgca tcccacccct agcaaatggc tgcaaaagcg gttgtacgat taccgcttct
      181 tcctcgcttt cgccgaacag gatgccgagg cgatgaaggc cgccttag
//
LOCUS       DAXIEB010000269          208 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000269 DAXIEB010000000
VERSION     DAXIEB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.269"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>208
                     /locus_tag="ACFOA3_002142"
     CDS             <1..>208
                     /locus_tag="ACFOA3_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFC6899067.1"
                     /translation="GWYDFRCQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAKSE
                     QRLSVLSEWVGQLETEYSELNLNRY"
BASE COUNT           51 a           54 c           55 g           48 t
ORIGIN      
        1 cggctggtac gatttccgct gccaaaacgt cgtgtacgcg cccgaccgca gcccggaaat
       61 cagccgtatg ctttcttcga ttttggcagg ctacgcgtgg gataccgaaa acccgttcgt
      121 ggcgaaatcc gaacaacgcc tgtcggtctt atcagaatgg gtcggtcagt tggaaaccga
      181 atatagtgaa ttaaatttaa accggtac
//
LOCUS       DAXIEB010000270          208 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000270 DAXIEB010000000
VERSION     DAXIEB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.270"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>208
                     /locus_tag="ACFOA3_002143"
     CDS             <1..>208
                     /locus_tag="ACFOA3_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6899068.1"
                     /translation="WQKIGGWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPN
                     PEGWIDDGHQCLAPWVKETKKRNG"
BASE COUNT           76 a           26 c           51 g           55 t
ORIGIN      
        1 gttggcaaaa aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg
       61 caaaaagaga gtggactgga atatttgaga tcgttaataa taacatcaag caatttagag
      121 atctgttccc aaatccggaa ggctggatcg atgatggtca ccaatgttta gctccttggg
      181 ttaaagaaac taaaaaacgc aatggcaa
//
LOCUS       DAXIEB010000271          208 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate 22ME000015
            SAMEA111315868-rid22928863.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAXIEB010000271 DAXIEB010000000
VERSION     DAXIEB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111315868
            Sequence Read Archive: ERR10149991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330809.1
            Genome Coverage       :: 351x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,144
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,980
            CDSs (with protein)               :: 1,980
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 81 of 114
            Pseudo Genes (internal stop)      :: 17 of 114
            Pseudo Genes (multiple problems)  :: 28 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111315868-rid22928863.denovo.271"
                     /isolate="22ME000015"
                     /isolation_source="eye secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Norway"
                     /collection_date="2022"
     gene            <1..>208
                     /locus_tag="ACFOA3_002144"
     CDS             <1..>208
                     /locus_tag="ACFOA3_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6899069.1"
                     /translation="YTGIDTETADQHTGRLKNSFDKDAVAKEINLQREVTKEFGRNAA
                     QAVAAVADKLGNTQSYERYQEARTL"
BASE COUNT           68 a           63 c           53 g           24 t
ORIGIN      
        1 tacaccggca tcgacaccga aactgcggat caacacacag gccgtctgaa aaacagcttc
       61 gacaaagacg cggtcgccaa agagatcaac ctgcaaaggg aagtaacgaa ggagttcggc
      121 agaaacgccg cccaagccgt agcggccgtt gccgacaaac tcggcaatac ccaaagttac
      181 gaacggtatc aggaagcccg aaccctgc
//
